title: Dataset of integrin-linked kinase protein: Protein interactions in cardiomyocytes identified by mass spectrometry.
abstract: Using hearts from mice overexpressing integrin linked kinase (ILK) behind the cardiac specific promoter αMHC, we have performed immunoprecipitation and mass spectrometry to identify novel ILK protein:protein interactions that regulate cardiomyocyte activity and calcium flux. Integrin linked kinase complexes were captured from mouse heart lysates using a commercial antibody, with subsequent liquid chromatography tandem mass spectral analysis. Interacting partners were identified using the MASCOT server, and important interactions verified using reverse immunoprecipitation and mass spectrometry. All ILK interacting proteins were identified in a non-biased manner, and are stored in the ProteomeXchange Consortium via the PRIDE partner repository (reference ID PRIDE: PXD001053). The functional role of identified ILK interactions in cardiomyocyte function and arrhythmia were subsequently confirmed in human iPSC-cardiomyocytes.
authors.affiliation: Division of Cardiovascular Surgery, Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Traister
authors.affiliation: Division of Cardiovascular Surgery, Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Lu
authors.affiliation: Division of Cardiovascular Surgery, Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8.
authors.forename: John G
authors.initials: JG
authors.lastname: Coles
authors.affiliation: Department of Anesthesia and Pain Medicine, Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8; Departments of Anesthesia and Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 2J7.
authors.forename: Jason T
authors.initials: JT
authors.lastname: Maynes
keywords: Calcium regulation; Cardiology; Mass spectrometry; Protein:protein interactions