title: Long-Term Tracking of the Osteogenic Differentiation of Mouse BMSCs by Aggregation-Induced Emission Nanoparticles.
abstract: Bone marrow-derived mesenchymal stem cells (BMSCs) have shown great potential for bone repair due to their strong proliferation ability and osteogenic capacity. To evaluate and improve the stem cell-based therapy, long-term tracking of stem cell differentiation into bone-forming osteoblasts is required. However, conventional fluorescent trackers such as fluorescent proteins, quantum dots, and fluorophores with aggregation-caused quenching (ACQ) characteristics have intrinsic limitations of possible interference with stem cell differentiation, heavy metal cytotoxicity, and self-quenching at a high labeling intensity. Herein, we developed aggregation-induced emission nanoparticles decorated with the Tat peptide (AIE-Tat NPs) for long-term tracking of the osteogenic differentiation of mouse BMSCs without interference of cell viability and differentiation ability. Compared with the ability of the commercial Qtracker 655 for tracking of only 6 passages of mouse BMSCs, AIE-Tat NPs have shown a much superior performance in long-term tracking for over 12 passages. Moreover, long-term tracking of the osteogenic differentiation process of mouse BMSCs was successfully conducted on the biocompatible hydroxyapatite scaffold, which is widely used in bone tissue engineering. Thus, AIE-Tat NPs have promising applications in tracking stem cell fate for bone repair.
authors.affiliation: State Key Laboratory of Luminescent Materials and Devices, South China University of Technology , Guangzhou 510640, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Gao
authors.affiliation: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology , Guangzhou 510640, China.
authors.forename: Junjian
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Luminescent Materials and Devices, South China University of Technology , Guangzhou 510640, China.
authors.forename: Gengwei
authors.initials: G
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Luminescent Materials and Devices, South China University of Technology , Guangzhou 510640, China.
authors.forename: Shiwu
authors.initials: S
authors.lastname: Li
authors.affiliation: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology , Guangzhou 510640, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Luminescent Materials and Devices, South China University of Technology , Guangzhou 510640, China.
authors.forename: Anjun
authors.initials: A
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Luminescent Materials and Devices, South China University of Technology , Guangzhou 510640, China.
authors.forename: Zujin
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology , Guangzhou 510640, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.affiliation: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology , Guangzhou 510640, China.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Luminescent Materials and Devices, South China University of Technology , Guangzhou 510640, China.|Department of Chemistry, The Hong Kong University of Science & Technology , Clear Water Bay, Kowloon, Hong Kong, China.|Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction , Hong Kong, China.
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D053758:Nanoparticles / Q000032:analysis* / Q000737:chemistry; D010012:Osteogenesis / Q000502:physiology*; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D023822:Tissue Engineering; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000032:analysis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D010446:Peptide Fragments; D054322:tat Gene Products, Human Immunodeficiency Virus
keywords: aggregation-induced emission; bone repair; long-term cell tracking; osteogenic differentiation; stem cell