title: Proliferation of mouse fibroblast-like and osteoblast-like cells on pure titanium films manufactured by electron beam melting.
abstract: The physical characteristics and biological compatibility of surfaces produced by electron beam melting (EBM) are not well known. In particular, there are not many reports on biocompatibility qualities. In this study, pure Ti films were manufactured using EBM. While it is reported that moderately hydrophilic biomaterial surfaces display improved cell growth and biocompatibility, contact angle measurements on the EBM-produced pure Ti films showed slight hydrophobicity. Nonetheless, we found the cell count of both fibroblast-like cells (L929) and osteoblast-like cells (MC3T3-E1) increased on pure Ti films, especially the MC3T3-E1, which increased more than that of the control. In addition, the morphology of L929 and MC3T3-E1 was polygonal and spindle-shaped and the cytoskeleton was well developed in the pure Ti surface groups. Upon staining with Alizarin red S, a slight calcium deposition was observed and this level gradually rose to a remarkable level. These results indicate that pure Ti films manufactured by EBM have good biocompatibility and could be widely applied as biomedical materials in the near future.
authors.affiliation: Department of Dental Materials Science, Aichi Gakuin University School of Dentistry, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 4648650, Japan.
authors.forename: Mayu
authors.initials: M
authors.lastname: Kawase
authors.affiliation: Department of Dental Materials Science, Aichi Gakuin University School of Dentistry, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 4648650, Japan. thayashi@dpc.agu.ac.jp.
authors.forename: Tatsuhide
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Department of Dental Materials Science, Aichi Gakuin University School of Dentistry, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 4648650, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Asakura
authors.affiliation: Department of Dental Materials Science, Aichi Gakuin University School of Dentistry, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 4648650, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Tomino
authors.affiliation: Department of Dental Materials Science, Aichi Gakuin University School of Dentistry, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 4648650, Japan.
authors.forename: Akimichi
authors.initials: A
authors.lastname: Mieki
authors.affiliation: Department of Dental Materials Science, Aichi Gakuin University School of Dentistry, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 4648650, Japan.
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Kawai
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004583:Electrons; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005615:Freezing; D008420:Manufactured Materials; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D014025:Titanium
keywords: additive manufacturing; biocompatibility; biomedical material; cell proliferation; electron beam melting; pure titanium film