title: Immunofluorescence Staining of Wt1 on Sections of Zebrafish Embryos and Larvae.
abstract: Immunohistochemistry is one of the most powerful tools for direct visualization of distribution and localization of gene products. The presented protocol provides an opportunity to determine the localization patterns of Wt1 in zebrafish via antibody staining.
authors.affiliation: Leibniz Institute for Age-Fritz Lipmann Institute, Beutenbergstrasse 11, 07745, Jena, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Perner
authors.affiliation: Leibniz Institute for Age-Fritz Lipmann Institute, Beutenbergstrasse 11, 07745, Jena, Germany. cenglert@fli-leibniz.de.|Friedrich Schiller University, Fürstengraben 1, 07743, Jena, Germany. cenglert@fli-leibniz.de.
authors.forename: Christoph
authors.initials: C
authors.lastname: Englert
mesh_terms: D000818:Animals; D016616:Cryoultramicrotomy; D005455:Fluorescent Antibody Technique / Q000379:methods*; D007668:Kidney / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D016610:Tissue Embedding; D025721:WT1 Proteins / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000378:metabolism
chemical_list: D025721:WT1 Proteins; D029961:Zebrafish Proteins
keywords: Cryosectioning; Immunohistochemistry; Kidney; Pronephros; Wt1