title: A novel phosphoserine motif in the LCMV matrix protein Z regulates the release of infectious virus and defective interfering particles.
abstract: We report that the lymphocytic choriomeningitis virus (LCMV) matrix protein, which drives viral budding, is phosphorylated at serine 41 (S41). A recombinant (r)LCMV bearing a phosphomimetic mutation (S41D) was impaired in infectious and defective interfering (DI) particle release, while a non-phosphorylatable mutant (S41A) was not. The S41D mutant was disproportionately impaired in its ability to release DI particles relative to infectious particles. Thus, DI particle production by LCMV may be dynamically regulated via phosphorylation of S41.
authors.affiliation: Department of Medicine, Division of Immunobiology, University of Vermont, Burlington, VT 05405, USA.|Cellular, Molecular and Biomedical Sciences Graduate Program, University of Vermont, Burlington, VT 05405, USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Ziegler
authors.affiliation: Department of Medicine, Division of Immunobiology, University of Vermont, Burlington, VT 05405, USA.
authors.forename: Philip
authors.initials: P
authors.lastname: Eisenhauer
authors.affiliation: Department of Medicine, Division of Immunobiology, University of Vermont, Burlington, VT 05405, USA.
authors.forename: Emily A
authors.initials: EA
authors.lastname: Bruce
authors.affiliation: Department of Medicine, Division of Immunobiology, University of Vermont, Burlington, VT 05405, USA.
authors.forename: Vedran
authors.initials: V
authors.lastname: Beganovic
authors.affiliation: Department of Medicine, Division of Immunobiology, University of Vermont, Burlington, VT 05405, USA.|Cellular, Molecular and Biomedical Sciences Graduate Program, University of Vermont, Burlington, VT 05405, USA.
authors.forename: Benjamin R
authors.initials: BR
authors.lastname: King
authors.affiliation: Department of Biology, University of Vermont, Burlington, VT 05405, USA.
authors.forename: Marion E
authors.initials: ME
authors.lastname: Weir
authors.affiliation: Department of Biology, University of Vermont, Burlington, VT 05405, USA.
authors.forename: Bryan A
authors.initials: BA
authors.lastname: Ballif
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA.|Department of Medicine, Division of Immunobiology, University of Vermont, Burlington, VT 05405, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Botten
mesh_terms: D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D003673:Defective Viruses / Q000378:metabolism*; D008217:Lymphocytic choriomeningitis virus / Q000502:physiology*; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D010768:Phosphoserine / Q000032:analysis*; D014763:Viral Matrix Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000378:metabolism*
chemical_list: D050505:Mutant Proteins; D014763:Viral Matrix Proteins; D010768:Phosphoserine