title: A comparative proteomics study of a synovial cell line stimulated with TNF-α.
abstract: To elucidate the pathogenesis of rheumatoid arthritis (RA), we used proteomic analysis to determine the protein profile in a synovial cell line, MH7A, established from patients with RA. Proteins were extracted from MH7A cells that were or were not stimulated with tumor necrosis factor-α (TNF-α), and then analyzed on a liquid chromatography/mass spectrometry system equipped with a unique long monolithic silica capillary. On the basis of the results of this proteomic analysis, we identified 2650 proteins from untreated MH7A cells and 2688 proteins from MH7A cells stimulated with TNF-α. Next, we selected 269 differentially produced proteins that were detected only under TNF-α stimulation, and classified these proteins by performing gene ontology analysis by using DAVID as a functional annotation tool. In TNF-α-stimulated MH7A cells, we observed substantial production of plasminogen-activator inhibitor 2 and apoptosis-regulating proteins such as BH3-interacting domain death agonist, autophagy protein 5, apolipoprotein E, and caspase-3. These results indicate that the upregulation of plasminogen-activator inhibitor 2 and apoptosis-regulating proteins in synovial cells in response to TNF-α stimulation might represent a predominant factor that contributes to the pathogenesis of RA.
authors.affiliation: General Education Center Hyogo University of Health Sciences Kobe Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Shibasaki
authors.affiliation: General Education Center Hyogo University of Health Sciences Kobe Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Karasaki
authors.affiliation: Division of Applied Life Science Graduate School of Agriculture Kyoto University Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Aburaya
authors.affiliation: Division of Applied Life Science Graduate School of Agriculture Kyoto University Japan.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Morisaka
authors.affiliation: Division of Applied Life Science Graduate School of Agriculture Kyoto University Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Takeda
authors.affiliation: Division of Applied Life Science Graduate School of Agriculture Kyoto University Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Aoki
authors.affiliation: Division of Rheumatology Department of Internal Medicine Hyogo College of Medicine Nishinomiya Japan.
authors.forename: Sachie
authors.initials: S
authors.lastname: Kitano
authors.affiliation: Division of Rheumatology Department of Internal Medicine Hyogo College of Medicine Nishinomiya Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kitano
authors.affiliation: Division of Applied Life Science Graduate School of Agriculture Kyoto University Japan.
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
authors.affiliation: Division of Rheumatology Department of Internal Medicine Hyogo College of Medicine Nishinomiya Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Sano
authors.affiliation: Division of Pharmacotherapy Department of Pharmacy School of Pharmacy Hyogo University of Health Sciences Kobe Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Iwasaki
keywords: TNF‐α; apoptosis; comparative proteomics; gene ontology analysis; rheumatoid arthritis; synovial cell line