title: In vivo ROS and redox potential fluorescent detection in plants: Present approaches and future perspectives.
abstract: Reactive oxygen species (ROS) are metabolic by-products in aerobic organisms including plants. Endogenously produced ROS act as cellular messengers and redox regulators involved in several plant biological processes, but excessive accumulation of ROS cause oxidative stress and cell damage. Understanding ROS signalling and stress responses requires precise imaging and quantification of local, subcellular and global ROS dynamics with high selectivity, sensitivity, and spatiotemporal resolution. Several fluorescent vital dyes have been tested so far, which helped to provide relevant spatially resolved information of oxidative stress dynamics in plants subjected to harmful environmental conditions. However, certain plant characteristics, such as high background fluorescence of plant tissues in vivo and antioxidant mechanisms, can interfere with ROS detection. The development of improved small-molecule fluorescent dyes and protein-based ROS sensors targeted to subcellular compartments will enable in vivo monitoring of ROS and redox changes in photosynthetic organisms.
authors.affiliation: Fisiología Vegetal (Plant Physiology Laboratory), Dpto. Biología (Biology Department), Universidad Autónoma de Madrid, 28049 Madrid, Spain. Electronic address: cristina.ortega@uam.es.
authors.forename: Cristina
authors.initials: C
authors.lastname: Ortega-Villasante
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Pozuelo de Alarcón, Madrid 28223, Spain.
authors.forename: Stefan
authors.initials: S
authors.lastname: Burén
authors.affiliation: Fisiología Vegetal (Plant Physiology Laboratory), Dpto. Biología (Biology Department), Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Barón-Sola
authors.affiliation: Fisiología Vegetal (Plant Physiology Laboratory), Dpto. Biología (Biology Department), Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Flor
authors.initials: F
authors.lastname: Martínez
authors.affiliation: Fisiología Vegetal (Plant Physiology Laboratory), Dpto. Biología (Biology Department), Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Luis E
authors.initials: LE
authors.lastname: Hernández
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010944:Plants / Q000737:chemistry / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species
keywords: Biosensors; Fluorescent probes; In vivo detection; Plant; Reactive oxygen species (ROS); Redox