title: How we do: optimizing bone marrow biopsy logistics for sign-out within 2 days.
abstract: Since the introduction of fast diagnostic tracks in many areas of oncology, the traditional processing of bone marrow biopsies (BMB), requiring either resin embedding or lengthy fixation and decalcification, is due to an upgrade. Thanks to a growing number of new commercially available tissue processors, microwave-enhanced processing is becoming a standard tool in the pathology laboratory, allowing rapid fixation and decalcification of BMB with preserved morphology and antigens. In this short report, we describe the use of a commercially available EDTA-based decalcification fluid (USEDECALC, Medite, Orlando, USA) in combination with the LOGOS J (Milestone, Bergamo, Italy), a closed microwave-enhanced tissue processor, for overnight fixation, decalcification, and paraffin impregnation of the BMB. This allows next-day reporting without impaired morphology or immunohistochemistry, and even improved DNA quality of the BMB.
authors.affiliation: Department of Pathology 824, Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: I
authors.initials: I
authors.lastname: de Laak-de Vries
authors.affiliation: Department of Pathology 824, Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: A G
authors.identifier: 0000-0002-6085-0678
authors.initials: AG
authors.lastname: Siebers
authors.affiliation: Department of Pathology 824, Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Burgers
authors.affiliation: Department of Pathology 824, Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Diepenbroek
authors.affiliation: Department of Pathology 824, Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Link
authors.affiliation: Department of Pathology 824, Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Groenen
authors.affiliation: Department of Pathology 824, Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: J H J M
authors.initials: JH
authors.lastname: van Krieken
authors.affiliation: Department of Pathology 824, Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: K M
authors.initials: KM
authors.lastname: Hebeda
keywords: Bone marrow examination; Decalcification technique