title: Anticancer Effect of Fucoidan on DU-145 Prostate Cancer Cells through Inhibition of PI3K/Akt and MAPK Pathway Expression.
abstract: In this study, we showed that PI3K/Akt signaling mediates fucoidan's anticancer effects on prostate cancer cells, including suppression of proliferation. Fucoidan significantly decreased viability of DU-145 cancer cells in a concentration-dependent manner as shown by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The drug also significantly increased chromatin condensation, which indicates apoptosis, in a concentration-dependent manner as shown by DAPI (4',6-diamidino-2-phenylindole) staining. Fucoidan increased expression of Bax, cleaved poly-ADP ribose polymerase and cleaved caspase-9, and decreased of the Bcl-2, p-Akt, p-PI3K, p-P38, and p-ERK in a concentration-dependent manner. In vivo, fucoidan (at 5 and 10 mg/kg) significantly decreased tumor volume, and increased apoptosis as assessed by the TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay, confirming the tumor inhibitory effect. The drug also increased expression of p-Akt and p-ERK as shown by immunohistochemistry staining. Therefore, fucoidan may be a promising cancer preventive medicine due to its growth inhibitory effects and induction of apoptosis in human prostate cancer cells.
authors.affiliation: Department of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, Korea. chu_0602@naver.com.
authors.forename: Gang-Sik
authors.initials: GS
authors.lastname: Choo
authors.affiliation: Department of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, Korea. lhn2726@naver.com.
authors.forename: Hae-Nim
authors.initials: HN
authors.lastname: Lee
authors.affiliation: Department of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, Korea. shinsaya@naver.com.
authors.forename: Seong-Ah
authors.initials: SA
authors.lastname: Shin
authors.affiliation: Department of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, Korea. tigershout@kongju.ac.kr.
authors.forename: Hyeong-Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, Korea. wangza@kongju.ac.kr.
authors.forename: Ji-Youn
authors.initials: JY
authors.lastname: Jung
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D020287:In Situ Nick-End Labeling / Q000379:methods; D007211:Indoles / Q000494:pharmacology; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D007211:Indoles; D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C007293:DAPI; C007789:fucoidan; D011065:Poly(ADP-ribose) Polymerases; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D053453:Caspase 9
keywords: Akt; apoptosis; fucoidan; human prostate cancer; mitogen-activated protein kinases; phosphoinositide 3-kinase