title: Multiphoton Microscopy for Visualizing Lipids in Tissue.
abstract: Visualizing the appearance of fat droplets and adipocytes in tissue can be realized using a label-free imaging method known as coherent anti-Stokes Raman spectroscopy (CARS). CARS is a nonlinear optical technique that allows label-free imaging of a material with contrast based on the same vibrational signatures of molecules found in Raman spectroscopy. CARS can be combined with other single and multiphoton imaging modes such as second harmonic generation and two-photon fluorescence to image a broad variety of biological structures.Here we describe the construction of a multiphoton microscope that will enable the study of both fluorescently labeled and unlabeled tissue. This has been used to monitor the contribution of Wt1 expressing cells towards the visceral fat depots during gestation.
authors.affiliation: Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, EH4 2XU, Scotland, UK. martin.lee@ed.ac.uk.
authors.forename: Martin
authors.initials: M
authors.lastname: Lee
authors.affiliation: Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, EH4 2XU, Scotland, UK.
authors.forename: Alan
authors.initials: A
authors.lastname: Serrels
mesh_terms: D000818:Animals; D005260:Female; D050152:Intra-Abdominal Fat / Q000378:metabolism / Q000648:ultrastructure; D008055:Lipids / Q000032:analysis*; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D011247:Pregnancy; D012097:Repressor Proteins / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods; D025721:WT1 Proteins
chemical_list: D008055:Lipids; D012097:Repressor Proteins; D025721:WT1 Proteins; C000602896:WT1 protein, mouse
keywords: CARS; Lipids; Microscopy; Multiphoton; Raman; SHG