title: miRNA expression profiling divides follicular dendritic cell sarcomas into two groups, related to fibroblasts and myopericytomas or Castleman's disease.
abstract: Follicular dendritic cell (FDC) sarcomas are rare mesenchymal tumours, which are fatal in 20% of the patients and usually occur in secondary lymphoid organs or extranodal localizations. Due to the rareness of these tumours, only few studies have been conducted on molecular level. In the present study, we performed microRNA (miRNA) profiling of 31 FDC sarcomas and identified two subgroups, one with high miRNA expression and the other group with low miRNA expression levels. The first group showed a strong similarity to fibroblasts and myopericytomas, whereas the second group was more closely related to FDCs from Castleman's disease. Both groups showed important differences compared with myeloid-derived dendritic cells, confirming mesenchymal origin of FDCs and their derived sarcomas. The two FDC sarcoma groups did not differ on morphological grounds, mitotic activity or BRAF mutation status. However, patients of group I presented a tendency to a shorter overall survival and more frequent podoplanin expression by immunohistochemistry. The importance of these newly recognized FDC sarcoma subgroups in terms of clinical behaviour and therapeutic implications should be assessed in a larger cohort in future studies.
authors.affiliation: Dr. Senckenberg Institute of Pathology, Goethe University, Frankfurt am Main, Germany. Electronic address: s.hartmann@em.uni-frankfurt.de.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Hartmann
authors.affiliation: Dr. Senckenberg Institute of Pathology, Goethe University, Frankfurt am Main, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Döring
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, Haematopathology Section, S. Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Agostinelli
authors.affiliation: Dr. Senckenberg Institute of Pathology, Goethe University, Frankfurt am Main, Germany.
authors.forename: Soo-Jeong
authors.initials: SJ
authors.lastname: Portscher-Kim
authors.affiliation: Pathology Unit, Department of Molecular and Translational Medicine, University of Brescia, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Lonardi
authors.affiliation: Pathology Unit, Department of Molecular and Translational Medicine, University of Brescia, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Lorenzi
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, Haematopathology Section, S. Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Fuligni
authors.affiliation: Hematopathology Section, Hospital Clinic, IDIBAPS, University of Barcelona, Barcelona, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Martinez
authors.affiliation: Histopathology, SRL Diagnostics, Mumbai 400013, India.
authors.forename: Jay
authors.initials: J
authors.lastname: Mehta
authors.affiliation: Histopathology, SRL Diagnostics, Mumbai 400013, India.
authors.forename: Anita
authors.initials: A
authors.lastname: Borges
authors.affiliation: Immunology, Gießen University Hospital, Gießen, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Hackstein
authors.affiliation: Department of Dermatology, Goethe University, Frankfurt am Main, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kippenberger
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, Haematopathology Section, S. Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy.
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Piccaluga
authors.affiliation: Institute of Pathology, Medical University of Vienna, Vienna, Austria.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Simonitsch-Klupp
authors.affiliation: Department of Pathology, Portuguese Institute of Oncology, Lisbon, Portugal.
authors.forename: José
authors.initials: J
authors.lastname: Cabeçadas
authors.affiliation: Hematopathology Section, Hospital Clinic, IDIBAPS, University of Barcelona, Barcelona, Spain.
authors.forename: Elias
authors.initials: E
authors.lastname: Campo
authors.affiliation: Pathology Unit, Department of Molecular and Translational Medicine, University of Brescia, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Facchetti
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, Haematopathology Section, S. Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy; Unit of Diagnostic Haematopathology, European Institute of Oncology, Milan, Italy.
authors.forename: Stefano A
authors.initials: SA
authors.lastname: Pileri
authors.affiliation: Dr. Senckenberg Institute of Pathology, Goethe University, Frankfurt am Main, Germany.
authors.forename: Martin-Leo
authors.initials: ML
authors.lastname: Hansmann
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D005871:Castleman Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D054740:Dendritic Cell Sarcoma, Follicular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020566:Dendritic Cells, Follicular / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D046228:Microarray Analysis; D008875:Middle Aged; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: BRAF; Cell of origin; Follicular dendritic cell sarcoma; Follicular dendritic cells; Podoplanin; miRNA profiling