title: Glycerol-fed microbial fuel cell with a co-culture of Shewanella oneidensis MR-1 and Klebsiella pneumonae J2B.
abstract: Glycerol is an attractive feedstock for bioenergy and bioconversion processes but its use in microbial fuel cells (MFCs) for electrical energy recovery has not been investigated extensively. This study compared the glycerol uptake and electricity generation of a co-culture of Shewanella oneidensis MR-1 and Klebsiella pneumonia J2B in a MFC with that of a single species inoculated counterpart. Glycerol was metabolized successfully in the co-culture MFC (MFC-J&M) with simultaneous electricity production but it was not utilized in the MR-1 only MFC (MFC-M). A current density of 10 mA/m(2) was obtained while acidic byproducts (lactate and acetate) were consumed in the co-culture MFC, whereas they are accumulated in the J2B-only MFC (MFC-J). MR-1 was distributed mainly on the electrode in MFC-J&M, whereas most of the J2B was observed in the suspension in the MFC-J reactor, indicating that the co-culture of both strains provides an ecological driving force for glycerol utilization using the electrode as an electron acceptor. This suggests that a co-culture MFC can be applied to electrical energy recovery from glycerol, which was previously known as a refractory substrate in a bioelectrochemical system.
authors.affiliation: School of Chemical and Biomolecular Engineering, Pusan National University, Busan, 609-735, Republic of Korea.
authors.forename: Changman
authors.initials: C
authors.lastname: Kim
authors.affiliation: School of Chemical and Biomolecular Engineering, Pusan National University, Busan, 609-735, Republic of Korea.
authors.forename: Young Eun
authors.initials: YE
authors.lastname: Song
authors.affiliation: School of Chemical and Biomolecular Engineering, Pusan National University, Busan, 609-735, Republic of Korea.
authors.forename: Cho Rong
authors.initials: CR
authors.lastname: Lee
authors.affiliation: Department of Natural Resources and Environmental Engineering, Hanyang University, Seoul, 133-791, Republic of Korea.
authors.forename: Byong-Hun
authors.initials: BH
authors.lastname: Jeon
authors.affiliation: School of Chemical and Biomolecular Engineering, Pusan National University, Busan, 609-735, Republic of Korea. j.kim@pusan.ac.kr.
authors.forename: Jung Rae
authors.identifier: 0000-0003-0103-7457
authors.initials: JR
authors.lastname: Kim
mesh_terms: D001674:Bioelectric Energy Sources; D018920:Coculture Techniques; D004566:Electrodes; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000502:physiology*; D020592:Shewanella / Q000378:metabolism / Q000502:physiology*
chemical_list: D005990:Glycerol
keywords: Co-culture; Glycerol; Klebsiella pneumoniae J2B; Microbial fuel cell; Shewanella oneidensis MR-1