title: Enhanced attached growth of microalgae Scenedesmus. LX1 through ambient bacterial pre-coating of cotton fiber carriers.
abstract: The role of bacteria/extracellular polymeric substances (EPS) coated carriers on attached microalgae growth in suspended-solid phase photobioreactor (sspBR) was assessed in this study. The results showed that pre-coating cotton with ambient bacteria and their EPS improved the attached microalgal growth by as much as 230% in terms of attached microalgae density. Additionally, the single cell dry weight, chemical composition and oxygen evolving activity of attached microalgae were significantly affected by the presence of bacteria/EPS coating on the cotton carriers. The protein content of microalgae cells cultivated in the ssPBRs with carriers coated by bacteria and sterilized bacteria were on average 26% and 15% more than uncoated carriers, respectively. Through absorbing and immobilizing nutrients from the bulk medium, the bacteria/EPS coating provided the attached microalgae with nitrogen/phosphorus for protein synthesis, especially during the late stages of batch cultivation.
authors.affiliation: Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing 100084, PR China; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Lin-Lan
authors.initials: LL
authors.lastname: Zhuang
authors.affiliation: Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Yaldah
authors.initials: Y
authors.lastname: Azimi
authors.affiliation: Department of Materials Science and Engineering, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Dawei
authors.initials: D
authors.lastname: Yu
authors.affiliation: Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing 100084, PR China.
authors.forename: Wen-Long
authors.initials: WL
authors.lastname: Wang
authors.affiliation: Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing 100084, PR China.
authors.forename: Yin-Hu
authors.initials: YH
authors.lastname: Wu
authors.affiliation: Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing 100084, PR China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Dao
authors.affiliation: Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing 100084, PR China; State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China. Electronic address: hyhu@tsinghua.edu.cn.
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Hu
mesh_terms: D001419:Bacteria; D032561:Cotton Fiber; D058086:Microalgae / Q000254:growth & development* / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D058433:Photobioreactors / Q000382:microbiology*; D044444:Scenedesmus / Q000254:growth & development* / Q000378:metabolism
chemical_list: D010758:Phosphorus; D009584:Nitrogen; D010100:Oxygen
keywords: Algal biofilm; Attached microalgae cultivation; Bacteria and extracellular polymeric substances (EPS) coated carrier; Harvesting microalgae; Suspended-solid phase photobioreactor (ssPBR)