title: Profiling the Membrane and Glycosaminoglycan-Binding Proteomes of Moraxella catarrhalis.
abstract: Moraxella catarrhalis, a Gram-negative bacterium, is an important respiratory pathogen causing acute otitis media and exacerbations of chronic obstructive pulmonary disease. Adhesion of the pathogen to human epithelial cells is mediated via bacterial membrane adhesin proteins. To identify the surface proteome of Moraxella catarrhalis, we applied different membrane protein extraction methods in combination with different proteomic technologies. Proteins from preparations of outer membrane vesicles and from carbonate extractions were analyzed using either a gel-based nano-HPLC-MS/MS technique or 2D-LC-MS/MS. Furthermore, because glycosaminoglycans (GAGs) play an important role for microbial entry into human cells, the GAG-binding membranome of Moraxella catarrhalis was investigated using a glycan-based pull-down approach. By these means, potential vaccine protein candidates that were previously selected by the ANTIGENome technology were confirmed, but importantly also novel proteins were identified as candidates.
authors.affiliation: Institute of Pharmaceutical Sciences, University of Graz , Universitaetsplatz 1, A-8010 Graz, Austria.
authors.forename: Anita
authors.initials: A
authors.lastname: Karner
authors.affiliation: Institute of Pharmaceutical Sciences, University of Graz , Universitaetsplatz 1, A-8010 Graz, Austria.
authors.forename: Bernd
authors.initials: B
authors.lastname: Gesslbauer
authors.affiliation: Institute of Pharmaceutical Sciences, University of Graz , Universitaetsplatz 1, A-8010 Graz, Austria.
authors.forename: Anton
authors.initials: A
authors.lastname: Spreitzer
authors.affiliation: Institute of Pharmaceutical Sciences, University of Graz , Universitaetsplatz 1, A-8010 Graz, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Almer
authors.affiliation: Valneva Austria GmbH , A-1030 Vienna, Austria.
authors.forename: Margarita
authors.initials: M
authors.lastname: Smidt
authors.affiliation: Valneva Austria GmbH , A-1030 Vienna, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schüler
authors.affiliation: Agowa GmbH , Berlin D-12459, Germany.
authors.forename: Berthold
authors.initials: B
authors.lastname: Fartmann
authors.affiliation: Agowa GmbH , Berlin D-12459, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Zimmermann
authors.affiliation: Valneva Austria GmbH , A-1030 Vienna, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Meinke
authors.affiliation: Institute of Pharmaceutical Sciences, University of Graz , Universitaetsplatz 1, A-8010 Graz, Austria.
authors.forename: Andreas J
authors.initials: AJ
authors.lastname: Kungl
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D001936:Moraxella catarrhalis / Q000737:chemistry*; D045828:Moraxellaceae Infections / Q000517:prevention & control / Q000628:therapy; D011485:Protein Binding; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D006025:Glycosaminoglycans; D020543:Proteome
keywords: GAG pull-down; Moraxella catarrhalis; bacterial adherence; glycosaminoglycans; otitis media; proteoglycans