title: Bacteria-Templated NiO Nanoparticles/Microstructure for an Enzymeless Glucose Sensor.
abstract: The bacterial-induced hollow cylinder NiO (HCNiO) nanomaterial was utilized for the enzymeless (without GOx) detection of glucose in basic conditions. The determination of glucose in 0.05 M NaOH solution with high sensitivity was performed using cyclic voltammetry (CV) and amperometry (i-t). The fundamental electrochemical parameters were analyzed and the obtained values of diffusion coefficient (D), heterogeneous rate constant (ks), electroactive surface coverage (Г), and transfer coefficient (alpha-α) are 1.75 × 10(-6) cm²/s, 57.65 M(-1)·s(-1), 1.45 × 10(-10) mol/cm², and 0.52 respectively. The peak current of the i-t method shows two dynamic linear ranges of calibration curves 0.2 to 3.5 µM and 0.5 to 250 µM for the glucose electro-oxidation. The Ni(2+)/Ni(3+) couple with the HCNiO electrode and the electrocatalytic properties were found to be sensitive to the glucose oxidation. The green chemistry of NiO preparation from bacteria and the high catalytic ability of the oxyhydroxide (NiOOH) is the good choice for the development of a glucose sensor. The best obtained sensitivity and limit of detection (LOD) for this sensor were 3978.9 µA mM(-1)·cm(-2) and 0.9 µM, respectively.
authors.affiliation: Department of Chemistry and Biochemistry, National Chung Cheng University, 168 University Road, Min-Hsiung, Chia-Yi 62102, Taiwan. svaidynathan@gmail.com.
authors.forename: Settu
authors.initials: S
authors.lastname: Vaidyanathan
authors.affiliation: Department of Chemistry and Biochemistry, National Chung Cheng University, 168 University Road, Min-Hsiung, Chia-Yi 62102, Taiwan. chejyc@ccu.edu.tw.
authors.forename: Jong-Yuh
authors.initials: JY
authors.lastname: Cherng
authors.affiliation: Department of Chemical Engineering &amp; Materials Science, Yuan Ze University. No. 135 Yuandong Road, Zhongli District, Taoyuan City 320, Taiwan. acsun@saturn.yzu.edu.tw.
authors.forename: An-Cheng
authors.initials: AC
authors.lastname: Sun
authors.affiliation: Department of Earth and Environmental Sciences, National Chung Cheng University, 168 University Road, Min-Hsiung, Chia-Yi 62102, Taiwan. chien-yen.chen@oriel.oxon.org.
authors.forename: Chien-Yen
authors.initials: CY
authors.lastname: Chen
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods* / Q000592:standards; D002138:Calibration; D002384:Catalysis; D055664:Electrochemical Techniques / Q000592:standards; D004566:Electrodes; D005947:Glucose / Q000032:analysis* / Q000592:standards; D007700:Kinetics; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D009532:Nickel; C028007:nickel monoxide; D005947:Glucose
keywords: amperometric sensors; electrocatalysis; electrochemical sensing; glassy carbon electrode (GCE); hollow cylinder NiO (HCNiO) nanostructure; non-enzymatic glucose sensor