title: The Neuroprotective Effects of Resveratrol Preconditioning in Transient Global Cerebral Ischemia-Reperfusion in Mice.
abstract: OBJECTIVE
  This study was designed to elucidate the neuroprotective effect of resveratrol in a mouse model of bilateral common carotid artery occlusion (BCCAO).
  
  
  METHODS
  Sixty male C57BL/6 mice, weighing 20-24 g, were used in our experiments. The mice were randomly assigned into three groups: control group, BCCAO group and BCCAO+Resveratrol group. Neurological score was assessed 24h, 48h, 72h after BCCAO, respectively. Hematoxylin and eosin (H&E) staining, NeuN and TUNEL were performed to detect the neuronal death and survival. The expression of Bcl-2, Bax, caspase-3, and cleaved caspase-3 were also detected to assess the anti-apoptotic effect of resveratrol by Western Blot.
  
  
  RESULTS
  Resveratrol significantly improved neurological score in BCCAO mice. Besides, it attenuates neuronal apoptosis via increasing the expression of Bcl-2 and decreasing the expression of Bax, caspase-3, and cleaved caspase-3. Resveratrol promotes neuronal survival in mice subjected to BCCAO.
  
  
  CONCLUSIONS
  Resveratrol is beneficial in the model of BCCAO, which is associated with its anti-apoptotic effect.
authors.affiliation: Jinan Central Hospital Affiliated to Shandong University, Department of Neurology, Jinan, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.forename: Jinbo
authors.initials: J
authors.lastname: Sun
authors.forename: Yanshuang
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D002546:Ischemic Attack, Transient / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D018696:Neuroprotective Agents; D013267:Stilbenes; D053148:Caspase 3; D000077185:Resveratrol