title: Amyloid-β peptide absence in short term effects on kinase activity of energy metabolism in mice hippocampus and cerebral cortex.
abstract: Considering that Alzheimer's disease is a prevalent neurodegenerative disease worldwide, we investigated the activities of three key kinases: creatine kinase, pyruvate kinase and adenylate kinase in the hippocampus and cerebral cortex in Alzheimer's disease model. Male adult Swiss mice received amyloid-β or saline. One day after, mice were treated with blank nanocapsules (17 ml/kg) or meloxicam-loaded nanocapsules (5 mg/kg) or free meloxicam (5 mg/kg). Treatments were performed on alternating days, until the end of the experimental protocol. In the fourteenth day, kinases activities were performed. Amyloid-β did not change the kinases activity in the hippocampus and cerebral cortex of mice. However, free meloxicam decrease the creatine kinase activity in mitochondrial-rich fraction in the group induced by amyloid-β, but for the cytosolic fraction, it has raised in the activity of pyruvate kinase activity in cerebral cortex. Further, meloxicam-loaded nanocapsules administration reduced adenylate kinase activity in the hippocampus of mice injected by amyloid-β. In conclusion we observed absence in short-term effects in kinases activities of energy metabolism in mice hippocampus and cerebral cortex using amyloid-β peptide model. These findings established the foundation to further study the kinases in phosphoryltransfer network changes observed in the brains of patients post-mortem with Alzheimer's disease.
authors.affiliation: Programa de Pós-Graduação em Nanociências, Centro de Ciências Tecnológicas, Centro Universitário Franciscano, Rua dos Andradas, 1614, Conjunto I, 97010-032 Santa Maria, RS, Brasil.
authors.forename: Francine R
authors.initials: FR
authors.lastname: Ianiski
authors.affiliation: Programa de Pós-Graduação em Nanociências, Centro de Ciências Tecnológicas, Centro Universitário Franciscano, Rua dos Andradas, 1614, Conjunto I, 97010-032 Santa Maria, RS, Brasil.
authors.forename: Virginia C
authors.initials: VC
authors.lastname: Rech
authors.affiliation: Programa de Pós-Graduação em Nanociências, Centro de Ciências Tecnológicas, Centro Universitário Franciscano, Rua dos Andradas, 1614, Conjunto I, 97010-032 Santa Maria, RS, Brasil.
authors.forename: Vivian S K
authors.initials: VS
authors.lastname: Nishihira
authors.affiliation: Programa de Pós-Graduação em Nanociências, Centro de Ciências Tecnológicas, Centro Universitário Franciscano, Rua dos Andradas, 1614, Conjunto I, 97010-032 Santa Maria, RS, Brasil.
authors.forename: Catiane B
authors.initials: CB
authors.lastname: Alves
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Av. Roraima, 1000, Cidade Universitária, Bairro Camobi, 97105-900 Santa Maria, RS, Brasil.
authors.forename: Matheus D
authors.initials: MD
authors.lastname: Baldissera
authors.affiliation: Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário, s/n, 96160-000 Capão do Leão, RS, Brasil.
authors.forename: Ethel A
authors.initials: EA
authors.lastname: Wilhelm
authors.affiliation: Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário, s/n, 96160-000 Capão do Leão, RS, Brasil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: Luchese
mesh_terms: D000263:Adenylate Kinase / Q000378:metabolism*; D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides; D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology*; D003402:Creatine Kinase / Q000378:metabolism*; D004734:Energy Metabolism; D006624:Hippocampus / Q000201:enzymology*; D006801:Humans; D008297:Male; D051379:Mice; D011770:Pyruvate Kinase / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D011770:Pyruvate Kinase; D003402:Creatine Kinase; D000263:Adenylate Kinase