title: [Preparation and Identification of High Immunogenic A/PR/8/34 Maternal Strain HA Protein for Influenza Virus Classical Reassortment].
abstract: Preparation of maternal strain A/PR/8/34 HA antiserum for influenza virus classical reassortment. A/PR/8/34 virus was digested by bromelain after inactivation and purification. 5%-20% sucrose continuous density gradient centrifugation method was used to purify HA protein. SIRD method was used to select the target protein. SDS-PAGE method was used to identified HA protein. High Immunogenic A/PR/8/34 HA protein was successfully prepared and HI titer reached 10240. High purity HA antiserum was identified by SIRD method. The key reagent in the classical reassortment of influenza virus was prepared, and the complete set of technical methods were explored, which laid the foundation for the independent research and development of seasonal influenza vaccine strains of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Xin
authors.forename: Junfeng
authors.initials: J
authors.lastname: Guo
authors.forename: Wenfei
authors.initials: W
authors.lastname: Zhu
authors.forename: Heyuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Shaohui
authors.initials: S
authors.lastname: Lang
authors.forename: Dayan
authors.initials: D
authors.lastname: Wang
authors.forename: Yuelong
authors.initials: Y
authors.lastname: Shu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000032:analysis* / Q000276:immunology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology / Q000821:virology*; D011817:Rabbits; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus