title: Molecular cloning and functional characterization of porcine E74-like factor 4 (ELF4).
abstract: E74-like factor 4 (ELF4) is a novel transcription factor that initiates transcription of type I interferon (IFN) genes to control diverse pathogens. Here, porcine ELF4 (poELF4) was cloned and its role in type I IFN signaling was investigated in different porcine cell lines. Full-length cDNA of poELF4 encodes 663 amino acid residues and ectopic expression of poELF4 significantly induced IFN-β production. Interestingly, difference from the human ELF4 (huELF4), poELF4 mutants lacking the serine/threonine rich domain, which has been demonstrated to be responsible for the phosphorylation of huELF4, were still capable of activating IFN-β promoter. Using pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV) as the models of DNA virus and RNA virus, respectively, we found that the replication of both PRV and PRRSV was reduced with poELF4 overexpression and enhanced with poELF4 knockdown. Taken together, these results suggested that poELF4 is an important antiviral host restriction factor.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. Electronic address: wangdang511@126.com.
authors.forename: Dang
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China.
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. Electronic address: fanglr@mail.hzau.edu.cn.
authors.forename: Liurong
authors.initials: L
authors.lastname: Fang
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate; D007370:Interferon Type I / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D000072417:Protein Domains / Q000235:genetics; D011557:Pseudorabies / Q000276:immunology*; D015398:Signal Transduction; D013552:Swine / Q000276:immunology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C491779:Elf4 protein, mouse; D007370:Interferon Type I; D014157:Transcription Factors
keywords: E74-like factor 4 (ELF4); Interferon; Porcine; Signaling pathway