title: Mutation of the key residue for extraribosomal function of ribosomal protein S19 cause increased grooming behaviors in mice.
abstract: Ribosomal protein S19 (RP S19) possesses ribosomal function as RP S19 monomer and extraribosomal function as cross-linked RP S19 oligomers which function as a ligand of the complement 5a (C5a) receptor (CD88). We have generated a Gln137Glu-RP S19 knock-in (KI) mouse, which is shown to possess the weakened extraribosomal function of RP S19. Because whether the extraribosomal function of RP S19 has a role in brain function had been unclear, we performed behavioral analysis on these mice and demonstrated that KI mice displayed an increased grooming behavior during open-field test and elevated plus maze test and an enhanced freezing behavior in contextual fear conditioning test. These results suggest an involvement of RP S19 oligomers in some anxiety-like behavior, especially grooming behavior.
authors.affiliation: Department of Molecular Pathology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Japan. Electronic address: kaitsuka@kumamoto-u.ac.jp.
authors.forename: Taku
authors.initials: T
authors.lastname: Kaitsuka
authors.affiliation: Department of Molecular Pathology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Fujino
authors.affiliation: Division of Developmental Genetics, Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
authors.forename: Kimi
authors.initials: K
authors.lastname: Araki
authors.affiliation: Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Tomizawa
authors.affiliation: Department of Molecular Pathology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D001007:Anxiety / Q000235:genetics* / Q000503:physiopathology*; D002633:Chemotaxis; D003214:Conditioning, Classical / Q000502:physiology; D005239:Fear / Q000502:physiology; D055879:Gene Knock-In Techniques; D006120:Grooming / Q000502:physiology*; D008297:Male; D008568:Memory / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009000:Monocytes / Q000502:physiology; D009154:Mutation; D012269:Ribosomal Proteins / Q000235:genetics* / Q000502:physiology*
chemical_list: D012269:Ribosomal Proteins; C040343:ribosomal protein S19
keywords: Anxiety-like behavior; Complement system; Extraribosomal function; Fear memory; Grooming behavior; Ribosomal protein S19