title: [Detection of Helicobacter pylori in children and adolescents using the monoclonal coproantigen immunoassay and its association with gastrointestinal diseases].
abstract: BACKGROUND
  Infection by Helicobacter pylori (H. pilory) affects 50% of the world population. Simple methods for its detection are now available.
  
  
  OBJECTIVE
  To identify H. pylori by using a monoclonal coproantigen technique in paediatric patients, and to determine its association with gastrointestinal diseases.
  
  
  METHODS
  The study included a total of 110 subjects aged 1 to 18 years. The study variables included: Family history of gastrointestinal disease, age, gender, gastrointestinal symptoms, as well as apparently healthy (asymptomatic) subjects. The monoclonal coproantigen test was performed on stool samples. Two groups, I symptomatic (n=29), and II asymptomatic (n=81) were compared using parametric and non-parametric statistics.
  
  
  RESULTS
  Of the 110 patients, 59 (54%) were male. The relationship between a family history of gastritis and a positive for H. pylori, was significant for mothers (p<0.0005), fathers (p<0.0001), and paternal grandfathers (p<0.0001). It was significant for gastric cancer in maternal grandparents (p<0.0178) and paternal grandparents (p<0.0092). The monoclonal coproantigen test was positive in 31 (28.2%) of the subjects. All were positive in group I, and only 2 in group II. A significant positive association was observed between H. pylori and various signs and symptoms, such as epigastric pain (p<0.001), recurrent peri-umbilical pain (p<0.001), bloating (p=0.016), heartburn (p=0.0007), nausea (P=0.0061), diarrhoea (p=0.0389), and constipation (p=0.0019).
  
  
  CONCLUSIONS
  H. pylori detection, was positive in 28% of both groups, and showed significant relationships with family gastrointestinal diseases and gastrointestinal symptoms.
authors.affiliation: Consulta Externa, Hospital Infantil del Estado de Sonora, Hermosillo, Sonora, México.
authors.forename: Verónica
authors.initials: V
authors.lastname: Castillo-Montoya
authors.affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, México.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Ruiz-Bustos
authors.affiliation: Departamento de Medicina y Ciencias de la Salud, Universidad de Sonora, Hermosillo, Sonora, México.
authors.forename: Mauro Eduardo
authors.initials: ME
authors.lastname: Valencia-Juillerat
authors.affiliation: Departamento de Medicina y Ciencias de la Salud, Universidad de Sonora, Hermosillo, Sonora, México.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Álvarez-Hernández
authors.affiliation: Departamento de Medicina y Ciencias de la Salud, Universidad de Sonora, Hermosillo, Sonora, México. Electronic address: norberto.sotelo@unison.mx.
authors.forename: Norberto
authors.initials: N
authors.lastname: Sotelo-Cruz
mesh_terms: D000293:Adolescent; D000942:Antigens, Bacterial / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005192:Family Health; D005243:Feces / Q000737:chemistry*; D005260:Female; D005756:Gastritis / Q000453:epidemiology / Q000382:microbiology; D005767:Gastrointestinal Diseases / Q000209:etiology*; D016481:Helicobacter Infections / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007223:Infant; D008297:Male; D010290:Parents; D013274:Stomach Neoplasms / Q000209:etiology; D063189:Symptom Assessment
chemical_list: D000942:Antigens, Bacterial
keywords: Coproantígeno monoclonal; Diagnosis; Diagnóstico; Helicobacter pylori; Infección en niños; Infection in children; Monoclonal coproantigen