title: Screening of Diatom Strains and Characterization of Cyclotella cryptica as A Potential Fucoxanthin Producer.
abstract: Fucoxanthin has been receiving ever-increasing interest due to its broad health beneficial effects. Currently, seaweeds are the predominant source of natural fucoxanthin. However, the disappointingly low fucoxanthin content has impeded their use, driving the exploration of alternative fucoxanthin producers. In the present study, thirteen diatom strains were evaluated with respect to growth and fucoxanthin production potential. Cyclotella cryptica (CCMP 333), which grew well for fucoxanthin production under both photoautotrophic and heterotrophic growth conditions, was selected for further investigation. The supply of nitrate and light individually or in combination were all found to promote growth and fucoxanthin accumulation. When transferring heterotrophic cultures to light, fucoxanthin responded differentially to light intensities and was impaired by higher light intensity with a concomitant increase in diadinoxanthin and diatoxanthin, indicative of the modulation of Diadinoxanthin Cycle to cope with the light stress. Taken together, we, for the first time, performed the screening of diatom strains for fucoxanthin production potential and investigated in detail the effect of nutritional and environmental factors on C. cryptica growth and fucoxanthin accumulation. These results provide valuable implications into future engineering of C. cryptica culture parameters for improved fucoxanthin production and C. cryptica may emerge as a promising microalgal source of fucoxanthin.
authors.affiliation: Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China. guobb@pku.edu.cn.
authors.forename: Bingbing
authors.initials: B
authors.lastname: Guo
authors.affiliation: Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China. caisanrenju@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China. ly_mikeyang@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China. zixinsp@163.com.
authors.forename: Peipei
authors.initials: P
authors.lastname: Sun
authors.affiliation: Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China. gracexuelu@pku.edu.cn.
authors.forename: Xue
authors.initials: X
authors.lastname: Lu
authors.affiliation: Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China. gjinliu@pku.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China. sfchencoe@pku.edu.cn.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou 510006, China. sfchencoe@pku.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
mesh_terms: D017377:Diatoms / Q000378:metabolism*; D008027:Light; D024341:Xanthophylls / Q000378:metabolism*
chemical_list: D024341:Xanthophylls; C025164:fucoxanthin; C033808:diadinoxanthin; C103105:diatoxanthin
keywords: Cyclotella cryptica; diatom; fucoxanthin; heterotrophic cultivation