title: Directing Biosynthesis: Practical Supply of Natural and Unnatural Cyanobactins.
abstract: The increasingly rapid accumulation of genomic information is revolutionizing natural products discovery. However, the translation of sequence data to chemical products remains a challenge. Here, we detail methods used to circumvent the supply problem of cyanobactin natural products, both by engineered synthesis in Escherichia coli and by using purified enzymes in vitro. Such methodologies exploit nature's strategies of combinatorial chemistry in the cyanobactin class of RiPP natural products. As a result, it is possible to synthesize a wide variety of natural and unnatural compounds.
authors.affiliation: University of Utah, Salt Lake City, UT, United States.
authors.forename: D
authors.initials: D
authors.lastname: Sardar
authors.affiliation: University of Utah, Salt Lake City, UT, United States.
authors.forename: M D
authors.initials: MD
authors.lastname: Tianero
authors.affiliation: University of Utah, Salt Lake City, UT, United States. Electronic address: ews1@utah.edu.
authors.forename: E W
authors.initials: EW
authors.lastname: Schmidt
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism*; D053898:Biosynthetic Pathways; D000458:Cyanobacteria / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D060847:Metabolic Engineering / Q000379:methods*; D005810:Multigene Family; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001688:Biological Products; D010456:Peptides, Cyclic
keywords: Diversity-generating metabolism; Marine natural products; Metabolic engineering; Ribosomally synthesized and posttranslationally modified peptide; Secondary metabolism