title: Fluorogenic Probes for Multicolor Imaging in Living Cells.
abstract: Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-permeable, and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.
authors.affiliation: Institute of Bioengineering, NCCR in Chemical Biology, Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL) , 1015 Lausanne, Switzerland.|Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry , Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Gražvydas
authors.initials: G
authors.lastname: Lukinavičius
authors.affiliation: Institute of Bioengineering, NCCR in Chemical Biology, Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL) , 1015 Lausanne, Switzerland.
authors.forename: Luc
authors.initials: L
authors.lastname: Reymond
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo , 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8654, Japan.
authors.forename: Keitaro
authors.initials: K
authors.lastname: Umezawa
authors.affiliation: Institute of Bioengineering, NCCR in Chemical Biology, Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL) , 1015 Lausanne, Switzerland.
authors.forename: Olivier
authors.initials: O
authors.lastname: Sallin
authors.affiliation: Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry , Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Elisa
authors.initials: E
authors.lastname: D'Este
authors.affiliation: Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry , Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Göttfert
authors.affiliation: Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry , Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Haisen
authors.initials: H
authors.lastname: Ta
authors.affiliation: Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry , Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo , 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8654, Japan.
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
authors.affiliation: Institute of Bioengineering, NCCR in Chemical Biology, Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL) , 1015 Lausanne, Switzerland.
authors.forename: Kai
authors.initials: K
authors.lastname: Johnsson
mesh_terms: D002470:Cell Survival; D003116:Color; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods*; D012235:Rhodamines / Q000737:chemistry; D012825:Silicon / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D012825:Silicon