title: Involvement of Acylated Homoserine Lactones (AHLs) of Aeromonas sobria in Spoilage of Refrigerated Turbot (Scophthalmus maximus L.).
abstract: One quorum sensing strain was isolated from spoiled turbot. The species was determined by 16S rRNA gene analysis and classical tests, named Aeromonas sobria AS7. Quorum-sensing (QS) signals (N-acyl homoserine lactones (AHLs)) were detected by report strains and their structures were further determined by GC-MS. The activity changes of AHLs on strain growth stage as well as the influence of different culture conditions on secretion activity of AHLs were studied by the punch method. The result indicated that strain AS7 could induce report strains to produce typical phenotypic response. N-butanoyl-dl-homoserine lactone (C₄-HSL), N-hexanoyl-dl-homoserine lactone (C₆-HSL), N-octanoyl-dl-homoserine lactone (C₈-HSL), N-decanoyl-dl-homoserine lactone (C10-HSL), N-dodecanoyl-dl-homoserine lactone (C12-HSL) could be detected. The activities of AHLs were density-dependent and the max secretion level was at pH 8, sucrose culture, 1% NaCl and 32 h, respectively. The production of siderophore in strain AS7 was regulated by exogenous C₈-HSL, rather than C₆-HSL. Exogenous C₄-HSL and C₈-HSL accelerated the growth rate and population density of AS7 in turbot samples under refrigerated storage. However, according to the total viable counts and total volatile basic nitrogen (TVB-N) values of the fish samples, exogenous C₆-HSL did not cause spoilage of the turbot fillets. In conclusion, our results suggested that QS was involved in the spoilage of refrigerated turbot.
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, China. jwltt@dlnu.edu.cn.|College of Life Science, Dalian Nationalities University, Dalian 116029, China. jwltt@dlnu.edu.cn.
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Food Science and Technology, Bohai University, Jinzhou 121013, China. cfc1031@163.com.
authors.forename: Fangchao
authors.initials: F
authors.lastname: Cui
authors.affiliation: College of Food Science and Technology, Bohai University, Jinzhou 121013, China. myan9102@163.com.
authors.forename: Fengling
authors.initials: F
authors.lastname: Bai
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, China. dotanicepl@163.com.
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhao
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, China. 13804242235@163.com.|College of Food Science and Technology, Bohai University, Jinzhou 121013, China. 13804242235@163.com.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Li
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis; D054742:Acyl-Butyrolactones / Q000032:analysis; D000333:Aeromonas / Q000737:chemistry*; D000818:Animals; D005412:Flatfishes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006714:Homoserine / Q000031:analogs & derivatives / Q000032:analysis; D007783:Lactones / Q000032:analysis; D053038:Quorum Sensing; D012336:RNA, Ribosomal, 16S
chemical_list: D054742:Acyl-Butyrolactones; D007783:Lactones; C092312:N-butyrylhomoserine lactone; C516096:N-dodecanoyl-DL-homoserine lactone; D012336:RNA, Ribosomal, 16S; C088386:homoserine lactone; D006714:Homoserine; D015107:4-Butyrolactone
keywords: Aeromonas sobria; quorum sensing; siderophore; spoilage; turbot