title: Altered expression of the bZIP transcription factor DRINK ME affects growth and reproductive development in Arabidopsis thaliana.
abstract: Here we describe an uncharacterized gene that negatively influences Arabidopsis growth and reproductive development. DRINK ME (DKM; bZIP30) is a member of the bZIP transcription factor family, and is expressed in meristematic tissues such as the inflorescence meristem (IM), floral meristem (FM), and carpel margin meristem (CMM). Altered DKM expression affects meristematic tissues and reproductive organ development, including the gynoecium, which is the female reproductive structure and is determinant for fertility and sexual reproduction. A microarray analysis indicates that DKM overexpression affects the expression of cell cycle, cell wall, organ initiation, cell elongation, hormone homeostasis, and meristem activity genes. Furthermore, DKM can interact in yeast and in planta with proteins involved in shoot apical meristem maintenance such as WUSCHEL, KNAT1/BP, KNAT2 and JAIBA, and with proteins involved in medial tissue development in the gynoecium such as HECATE, BELL1 and NGATHA1. Taken together, our results highlight the relevance of DKM as a negative modulator of Arabidopsis growth and reproductive development.
authors.affiliation: Unidad de Genómica Avanzada (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, Gto., México.
authors.forename: Paulina
authors.initials: P
authors.lastname: Lozano-Sotomayor
authors.affiliation: Unidad de Genómica Avanzada (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, Gto., México.
authors.forename: Ricardo A
authors.identifier: 0000-0001-6132-8956
authors.initials: RA
authors.lastname: Chávez Montes
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Campus de la Universidad Politécnica de Valencia, 46022, Valencia, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Silvestre-Vañó
authors.affiliation: Unidad de Genómica Avanzada (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, Gto., México.
authors.forename: Humberto
authors.initials: H
authors.lastname: Herrera-Ubaldo
authors.affiliation: Rice Genomics Group, Parco Tecnologico Padano, Lodi, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Greco
authors.affiliation: Unidad de Genómica Avanzada (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, Gto., México.
authors.forename: Jeanneth
authors.initials: J
authors.lastname: Pablo-Villa
authors.affiliation: Dipartimento di Bioscienze, Università degli Studi di Milano, Milan, Italy.
authors.forename: Bianca M
authors.initials: BM
authors.lastname: Galliani
authors.affiliation: Departamento de Biotecnología y Bioquímica, CINVESTAV-IPN, Irapuato, Gto., México.
authors.forename: David
authors.initials: D
authors.lastname: Diaz-Ramirez
authors.affiliation: Wageningen University and Research Centre, Bioscience, P.O. Box 619, 6700 AP, Wageningen, The Netherlands.
authors.forename: Mieke
authors.initials: M
authors.lastname: Weemen
authors.affiliation: Wageningen University and Research Centre, Bioscience, P.O. Box 619, 6700 AP, Wageningen, The Netherlands.
authors.forename: Kim
authors.initials: K
authors.lastname: Boutilier
authors.affiliation: Crop, Soil and Environmental Sciences, University of Arkansas, 115 Plant Science Building, Fayetteville, AR, 72701, USA.
authors.forename: Andy
authors.initials: A
authors.lastname: Pereira
authors.affiliation: Dipartimento di Bioscienze, Università degli Studi di Milano, Milan, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Colombo
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Campus de la Universidad Politécnica de Valencia, 46022, Valencia, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Madueño
authors.affiliation: Departamento de Biotecnología y Bioquímica, CINVESTAV-IPN, Irapuato, Gto., México.
authors.forename: Nayelli
authors.initials: N
authors.lastname: Marsch-Martínez
authors.affiliation: Unidad de Genómica Avanzada (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, Gto., México.
authors.forename: Stefan
authors.initials: S
authors.lastname: de Folter
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D018519:Meristem / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D014157:Transcription Factors
keywords: 
  Arabidopsis thaliana
  ; DRINK ME; bZIP transcription factor; bZIP30; growth; meristem activity; reproductive development