title: Nucleic Acid Templated Chemical Reaction in a Live Vertebrate.
abstract: Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living tissues of a vertebrate.
authors.affiliation: School of Chemistry and Biochemistry, NCCR Chemical Biology, University of Geneva , 30 quai Ernest Ansermet, Geneva, Switzerland.
authors.forename: Laurent
authors.initials: L
authors.lastname: Holtzer
authors.affiliation: School of Chemistry and Biochemistry, NCCR Chemical Biology, University of Geneva , 30 quai Ernest Ansermet, Geneva, Switzerland.
authors.forename: Igor
authors.initials: I
authors.lastname: Oleinich
authors.affiliation: School of Chemistry and Biochemistry, NCCR Chemical Biology, University of Geneva , 30 quai Ernest Ansermet, Geneva, Switzerland.
authors.forename: Marcello
authors.initials: M
authors.lastname: Anzola
authors.affiliation: School of Chemistry and Biochemistry, NCCR Chemical Biology, University of Geneva , 30 quai Ernest Ansermet, Geneva, Switzerland.
authors.forename: Eric
authors.initials: E
authors.lastname: Lindberg
authors.affiliation: School of Chemistry and Biochemistry, NCCR Chemical Biology, University of Geneva , 30 quai Ernest Ansermet, Geneva, Switzerland.
authors.forename: Kalyan K
authors.initials: KK
authors.lastname: Sadhu
authors.affiliation: School of Chemistry and Biochemistry, NCCR Chemical Biology, University of Geneva , 30 quai Ernest Ansermet, Geneva, Switzerland.
authors.forename: Marcos
authors.initials: M
authors.lastname: Gonzalez-Gaitan
authors.affiliation: School of Chemistry and Biochemistry, NCCR Chemical Biology, University of Geneva , 30 quai Ernest Ansermet, Geneva, Switzerland.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Winssinger