title: Theileria annulata seroprevalence among different cattle breeds in Rajshahi Division, Bangladesh.
abstract: An epidemiological survey of Theileria annulata infection was undertaken in a cattle population in Rajshahi Division, Bangladesh. The local cattle breeds from the area (North Bengal Gray and Deshi) and crosses between the local breeds and Holstein cattle were predominantly screened. In total, 192 cattle serum samples were collected in two areas of Rajshahi Division, the Rajshahi District (n=147) and Natore District (n=45). The samples were screened with an enzyme-linked immunosorbent assay using T. annulata surface protein (TaSP) as the antigen. The seroprevalence was 80.0% (36/45) in Natore and 20.4% (30/147) in Rajshahi. A logistic regression analysis showed that the sampling location was significantly associated with seropositivity, whereas age, sex and breed were not. Although the logistic regression analysis did not show a linear dependence on age, we considered age-specific seroprevalence separately in the two districts. Seroprevalence did not differ significantly among age categories in the Natore District. In contrast, all the cattle <1 year old in the Rajshahi District were seronegative (11/11). Seroprevalence in the 1- and 2-year-old cattle was significantly lower in the Rajshahi District than in the Natore District. In the older age categories (3, 4 and >5 years), seroprevalence did not differ significantly between the Natore and Rajshahi Districts. These results suggest that the cattle in the Rajshahi District were sporadically exposed to T. annulata, whereas most cattle in the Natore District became infected during an early phase of life.
authors.affiliation: Department of Animal Husbandry and Veterinary Science, Faculty of Agriculture, University of Rajshahi, Rajshahi-6205, Bangladesh.
authors.forename: Md Wajed
authors.initials: MW
authors.lastname: Ali
authors.forename: Md
authors.initials: M
authors.lastname: Alauddin
authors.forename: Md Thoufic Anam
authors.initials: MT
authors.lastname: Azad
authors.forename: Md Ariful
authors.initials: MA
authors.lastname: Hasan
authors.forename: Cornelia
authors.initials: C
authors.lastname: Appiah-Kwarteng
authors.forename: Masaki
authors.initials: M
authors.lastname: Takasu
authors.forename: Minami
authors.initials: M
authors.lastname: Baba
authors.forename: Katsuya
authors.initials: K
authors.lastname: Kitoh
authors.forename: Moizur
authors.initials: M
authors.lastname: Rahman
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takashima
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001459:Bangladesh / Q000453:epidemiology; D002417:Cattle; D005260:Female; D008297:Male; D016036:Seroepidemiologic Studies; D016796:Theileria annulata / Q000276:immunology; D013801:Theileriasis / Q000453:epidemiology*
chemical_list: D000913:Antibodies, Protozoan