title: Association of Promoter Polymorphisms of Interleukin-10 and Interferon-Gamma Genes with Tuberculosis in Azeri Population of Iran.
abstract: Promoter polymorphism of cytokine genes may lead to inter-individual differences in cytokine levels, therefore, polymorphisms may associate with susceptibility to infectious diseases. In this study, we investigated a possible association between interleukin-10 (IL-10) -1082A⁄G (rs1800896) and interferon (IFN)-gamma +874T/A (rs2430561) promoter polymorphisms and tuberculosis (TB) in the Azeri population of Iran. IL-10 -1082G/A and IFN-gamma +874T/A single nucleotide polymorphisms (SNPs) were genotyped by amplification refractory mutation system (ARMS)-PCR in 200 healthy controls and 124 tuberculosis patients. IL-10 -1082 A allele was more frequent in the control group than in the patient group (p=0.001, odds ratio [OR]=2.183). On the other hand, the AA genotype was significantly more frequent in the control group (p=0.0001). The frequency of IFN-gamma +874 T allele was significantly higher in the controls (p=0.013, OR=1.56). There was no significant association between IFN-gamma +874 T/A genotypes and susceptibility to tuberculosis (p=0.078), but TT genotype was more frequent in the control group. Our findings suggest that interleukin-10 -1082G/A polymorphism may play an important role in susceptibility to tuberculosis in our population. On the other hand, the +874T allele, which has been suggested to be associated with high IFN-gamma levels, was significantly higher in the controls and TT genotype was also more frequent in the control group. Thus, +874 T allele may be associated with resistance to tuberculosis in this Azeri population of Iran.
authors.affiliation: Department of Laboratory Sciences, Faculty of Paramedical, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Asgharzadeh
authors.affiliation: Biotechnology Research Center, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Sajjad
authors.initials: S
authors.lastname: Ghorghanlu
authors.affiliation: Department of Laboratory Sciences, Faculty of Paramedical, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Jalil
authors.initials: J
authors.lastname: Rashedi
authors.affiliation: Department of Laboratory Sciences, Faculty of Paramedical, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Behroz
authors.initials: B
authors.lastname: Mahdavi Poor
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Khaki-Khatibi
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Seyyed Reza
authors.initials: SR
authors.lastname: Moaddab
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Samadi-Kafil
authors.affiliation: Infectious Disease and Tropical Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mahya
authors.initials: M
authors.lastname: Pourostadi
mesh_terms: D000328:Adult; D000483:Alleles; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D007492:Iran / Q000453:epidemiology / Q000208:ethnology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D014376:Tuberculosis / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics* / Q000276:immunology
chemical_list: C573415:IFNG protein, human; C508609:IL10 protein, human; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: Association; Interferon-gamma; Interleukin-10; Polymorphism; Susceptibility; Tuberculosis