title: Treadmill exercise induces age and protocol-dependent epigenetic changes in prefrontal cortex of Wistar rats.
abstract: Some studies have linked age-related beneficial effects of exercise and epigenetic mechanisms. Although, the impact of treadmill exercise on histone acetylation, histone and DNA methylation marks in aged cortices yet remains poorly understood. Considering the role of frontal cortex on brain functions, we investigated the potential of different exercise protocols, single session and daily exercise, to modulate epigenetic marks, namely global H4 acetylation, histone methyltransferase activity (HMT H3K27) and levels of DNA methytransferase (DNMT1 and DNMT3b) in prefrontal cortices from 3 and 21-months aged Wistar rats. The animals were submitted to two treadmill exercise protocols, single session (20min) or daily moderate (20min/day during 14days). The daily exercise protocol induced an increased in histone H4 acetylation levels in prefrontal cortices of 21-months-old rats, without any effects in young adult group. DNMT3b levels were increased in aged cortices of animals submitted to single session of exercise. These results indicate that prefrontal cortex is susceptible to epigenetic changes in a protocol dependent-manner and that H4 acetylation levels and DNMT3b content changes might be linked at least in part to exercise-induced effects on brain functions.
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Laura Reck
authors.initials: LR
authors.lastname: Cechinel
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Carla Giovana
authors.initials: CG
authors.lastname: Basso
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Karine
authors.initials: K
authors.lastname: Bertoldi
authors.affiliation: Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Bruna
authors.initials: B
authors.lastname: Schallenberger
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Louisiana Carolina Ferreira
authors.initials: LCF
authors.lastname: de Meireles
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Brazil; Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Brazil. Electronic address: ionara@ufrgs.br.
authors.forename: Ionara Rodrigues
authors.initials: IR
authors.lastname: Siqueira
mesh_terms: D000107:Acetylation; D000818:Animals; D000076123:DNA (Cytosine-5-)-Methyltransferase 1 / Q000378:metabolism; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism; D044127:Epigenesis, Genetic; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D008297:Male; D009043:Motor Activity; D017397:Prefrontal Cortex / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000097584:DNA Methyltransferase 3B
chemical_list: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; D006657:Histones; D000097584:DNA Methyltransferase 3B; C000619382:Dnmt1 protein, rat
keywords: Aging; Cortex; DNA methyltransferase; Histone acetylation; Treadmill exercise