title: Diagnostic potential of tumor DNA from ovarian cyst fluid.
abstract: We determined whether the mutations found in ovarian cancers could be identified in the patients' ovarian cyst fluids. Tumor-specific mutations were detectable in the cyst fluids of 19 of 23 (83%) borderline tumors, 10 of 13 (77%) type I cancers, and 18 of 18 (100%) type II cancers. In contrast, no mutations were found in the cyst fluids of 18 patients with benign tumors or non-neoplastic cysts. Though large, prospective studies are needed to demonstrate the safety and clinical utility of this approach, our results suggest that the genetic evaluation of cyst fluids might be able to inform the management of the large number of women with these lesions.
authors.affiliation: Ludwig Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Sidney Kimmel Comprehensive Cancer Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.
authors.forename: Yuxuan
authors.identifier: 0000-0002-2932-6042
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Clinical Sciences, Sahlgrenska Cancer Center, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Sundfeldt
authors.affiliation: Department of Pathology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Constantina
authors.initials: C
authors.lastname: Mateoiu
authors.affiliation: Department of Pathology, The Johns Hopkins Medical Institutes, Baltimore, United States.|Department of Gynecology and Obstetrics, The Johns Hopkins Medical Institutions, Baltimore, United States.
authors.forename: Ie-Ming
authors.initials: IeM
authors.lastname: Shih
authors.affiliation: Department of Pathology, The Johns Hopkins Medical Institutes, Baltimore, United States.|Department of Gynecology and Obstetrics, The Johns Hopkins Medical Institutions, Baltimore, United States.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Kurman
authors.affiliation: Ludwig Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Sidney Kimmel Comprehensive Cancer Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.
authors.forename: Joy
authors.initials: J
authors.lastname: Schaefer
authors.affiliation: Ludwig Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Sidney Kimmel Comprehensive Cancer Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.
authors.forename: Natalie
authors.initials: N
authors.lastname: Silliman
authors.affiliation: Ludwig Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Sidney Kimmel Comprehensive Cancer Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.
authors.forename: Isaac
authors.initials: I
authors.lastname: Kinde
authors.affiliation: Ludwig Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Sidney Kimmel Comprehensive Cancer Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.
authors.forename: Simeon
authors.initials: S
authors.lastname: Springer
authors.affiliation: Ludwig Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Sidney Kimmel Comprehensive Cancer Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Swim Across America Laboratory, Baltimore, United States.
authors.forename: Michael
authors.initials: M
authors.lastname: Foote
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Clinical Sciences, Sahlgrenska Cancer Center, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Björg
authors.initials: B
authors.lastname: Kristjansdottir
authors.affiliation: Ludwig Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Sidney Kimmel Comprehensive Cancer Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.
authors.forename: Nathan
authors.initials: N
authors.lastname: James
authors.affiliation: Ludwig Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Sidney Kimmel Comprehensive Cancer Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Kinzler
authors.affiliation: Ludwig Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Sidney Kimmel Comprehensive Cancer Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.
authors.forename: Nickolas
authors.initials: N
authors.lastname: Papadopoulos
authors.affiliation: Ludwig Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Sidney Kimmel Comprehensive Cancer Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Swim Across America Laboratory, Baltimore, United States.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Diaz
authors.affiliation: Ludwig Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.|Sidney Kimmel Comprehensive Cancer Center, Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, United States.
authors.forename: Bert
authors.identifier: 0000-0003-0766-3854
authors.initials: B
authors.lastname: Vogelstein
mesh_terms: D020420:Cyst Fluid / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D009154:Mutation; D010048:Ovarian Cysts / Q000175:diagnosis* / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D011446:Prospective Studies
chemical_list: D004247:DNA
keywords: biomarker; cancer biology; human biology; medicine; none; ovarian cancer; tumor DNA