title: L-proline: a highly effective cryoprotectant for mouse oocyte vitrification.
abstract: Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied to mammalian oocyte vitrification. In this study we evaluated the efficiency and safety of the new cryoprotectant in oocyte vitrification. The results indicated that L-proline improves the survival rate of vitrified oocytes, protects mitochondrial functions and could be applied as a new cryoprotectant in mouse oocyte vitrification.
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, No. 49 North Hua Yuan Road, Hai Dian District, Beijing 100191, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing 100191, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Key Lab of Cryo-Biomedical Engineering and Key Lab of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Xue
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, No. 49 North Hua Yuan Road, Hai Dian District, Beijing 100191, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing 100191, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yan
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, No. 49 North Hua Yuan Road, Hai Dian District, Beijing 100191, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Yan
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, No. 49 North Hua Yuan Road, Hai Dian District, Beijing 100191, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing 100191, China.
authors.forename: Xiao-Hu
authors.initials: XH
authors.lastname: Jin
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, No. 49 North Hua Yuan Road, Hai Dian District, Beijing 100191, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing 100191, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Zhu
authors.affiliation: Beijing Key Lab of Cryo-Biomedical Engineering and Key Lab of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhi-Zhu
authors.initials: ZZ
authors.lastname: He
authors.affiliation: Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing 100191, China.|Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, No. 49 North Hua Yuan Road, Hai Dian District, Beijing 100191, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, No. 49 North Hua Yuan Road, Hai Dian District, Beijing 100191, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D047108:Embryonic Development / Q000187:drug effects; D019855:Ethylene Glycol / Q000008:administration & dosage; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D011392:Proline / Q000008:administration & dosage / Q000494:pharmacology*; D020554:Sperm Injections, Intracytoplasmic; D008941:Spindle Apparatus / Q000187:drug effects / Q000648:ultrastructure; D058989:Vitrification / Q000187:drug effects*
chemical_list: D003451:Cryoprotective Agents; D011392:Proline; D019855:Ethylene Glycol; D004121:Dimethyl Sulfoxide