title: Correlations of platelet-leukocyte aggregates with P-selectin S290N and P-selectin glycoprotein ligand-1 M62I genetic polymorphisms in patients with acute ischemic stroke.
abstract: BACKGROUND
  Platelet-leukocyte aggregations (PLA) play key roles in acute ischemic stroke (AIS) process and they are formed by the combination of P-selectin (SELP) expressed on the surface of the platelet membranes with P-selectin glycoprotein ligand-1(PSGL-1) expressed on the surface of the leukocytes. There are genetic polymorphisms in SELP and PSGL-1. We tested the differences in all kinds of PLA among subtypes of AIS and the association of SELP S290N and PSGL-1 M62I polymorphism with AIS to assess the correlations of PLA with SELP S290N and PSGL-1 M62I genetic polymorphisms.
  
  
  METHODS
  One hundred and forty-eight patients with acute ischemic stroke, including thirty patients with large artery atherosclerosis stroke (LAA), twenty-four patients with cardioembolism (CE) and ninety-four patients with small artery occlusion stroke (SAO), and eighty-eight control subjects were evaluated. The lab parameters and levels of PLA were measured within 3h after the ischemic event in the case subjects and within empty stomach in the control subjects. In all subjects, SELP S290N and PSGL-1 M62I polymorphisms were also measured.
  
  
  RESULTS
  Platelet-monocyte aggregates (PMA), platelet-lymphocyte aggregates (PLyA) and platelet-neutrophil aggregates (PNA) in LAA group, CE group and SAO group were all higher than that in control group (P=0.000 for all comparisons). Compared with LAA group, PMA and PLyA in CE group were significantly higher (P=0.018 and P=0.003, respectively). Compared with SAO group, PMA and PLyA in CE group were also significantly higher (P=0.041 and P=0.019, respectively). Compared with control group, there were significantly differences of I allele frequencies of PSGL-1 M62I in LAA group and SAO group (OR=2.249, 95%CI: 1.175-4.304, P=0.021 and OR=2.055, 95%CI: 1.269-3.328, P=0.004, respectively). In SAO group, there were significantly differences of PNA among MM, MI and II genotype of PSGL-1 M62I (P=0.008).
  
  
  CONCLUSIONS
  PLA increased in AIS patients rapidly within 3h. The I allele of PSGL-1 M62I was associated with risk of developing AIS, especially LAA and SAO. SAO patients with the II genotype of PSGL-1 M62I have the higher level of PNA.
authors.affiliation: Department of Laboratory Medicine, Jingzhou Central Hospital, the Second Clinical Medical College, Yangtze University, Jingzhou, Hubei Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tao
authors.affiliation: Department of Laboratory Medicine, Jingzhou Central Hospital, the Second Clinical Medical College, Yangtze University, Jingzhou, Hubei Province, China. Electronic address: wangchangfu16888@hotmail.com.
authors.forename: Wang
authors.initials: W
authors.lastname: Changfu
authors.affiliation: Department of Laboratory Medicine, Jingzhou Central Hospital, the Second Clinical Medical College, Yangtze University, Jingzhou, Hubei Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Linyun
authors.affiliation: Department of Laboratory Medicine, Jingzhou Central Hospital, the Second Clinical Medical College, Yangtze University, Jingzhou, Hubei Province, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Bing
authors.affiliation: Department of Neurology, Jingzhou Central Hospital, the Second Clinical Medical College, Yangtze University, Jingzhou, Hubei Province, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Xiaohui
mesh_terms: D000368:Aged; D002545:Brain Ischemia / Q000097:blood* / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D005787:Gene Frequency; D060005:Genotyping Techniques; D006801:Humans; D002537:Intracranial Arteriosclerosis / Q000097:blood / Q000235:genetics; D007962:Leukocytes / Q000502:physiology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D019007:P-Selectin / Q000235:genetics*; D010974:Platelet Aggregation / Q000235:genetics / Q000502:physiology; D011110:Polymorphism, Genetic; D020521:Stroke / Q000097:blood* / Q000235:genetics*
chemical_list: D008562:Membrane Glycoproteins; D019007:P-Selectin; C084882:P-selectin ligand protein; C546937:SELP protein, human
keywords: Acute ischemic stroke; Gene polymorphism; P-selectin; P-selectin glycoprotein ligand-1; Platelet-monocyte aggregates; Platelet-neutrophil aggregates; Subtype