title: Tools and Techniques for Wt1-Based Lineage Tracing.
abstract: The spatiotemporal expression pattern of Wt1 has been extensively studied in a number of animal models to establish its function and the developmental fate of the cells expressing this gene. In this chapter, we review the available animal models for Wt1-expressing cell lineage analysis, including direct Wt1 expression reporters and systems for permanent Wt1 lineage tracing. We describe the presently used constitutive or inducible genetic lineage tracing approaches based on the Cre/loxP system utilizing Cre recombinase expression under control of a Wt1 promoter.To make these systems accessible, we provide laboratory protocols that include dissection and processing of the tissues for immunofluorescence and histopathological analysis of the lineage-labeled Wt1-derived cells within the embryo/tissue context.
authors.affiliation: Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, UK. b.wilm@liv.ac.uk.
authors.forename: Bettina
authors.initials: B
authors.lastname: Wilm
authors.affiliation: Department of Animal Biology, Faculty of Science, University of Malaga, Malaga, Spain. chapuli@uma.es.
authors.forename: Ramon
authors.initials: R
authors.lastname: Muñoz-Chapuli
mesh_terms: D000818:Animals; D019070:Cell Lineage; D058948:Cell Tracking / Q000379:methods*; D017930:Genes, Reporter; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D013629:Tamoxifen / Q000494:pharmacology; D025721:WT1 Proteins
chemical_list: D012097:Repressor Proteins; D025721:WT1 Proteins; C000602896:WT1 protein, mouse; D013629:Tamoxifen; C045073:Cre recombinase; D019426:Integrases
keywords: Cell lineage tracing; Cre-ERT2; Cre/loxP; Inducible Cre recombinase; Rosa26 reporter mice; Tamoxifen; Wt1