title: The effect of leptin on the respiratory burst of human neutrophils cultured in synovial fluid.
abstract: OBJECTIVE
  Leptin is a hormone responsible for nutritional status and immune competence coordination. In rheumatoid arthritis (RA) increased leptin levels were observed in both serum and synovial fluid. Its influence on development of the disease still remains unclear. So far, research on leptin's influence on the emission of reactive oxygen intermediates (ROI) measured with chemiluminescence (CL) has provided unclear and contradictory results. In this study, we evaluated the influence of leptin on oxidative activity of neutrophils isolated from blood of healthy volunteers and cultured in different amounts of synovial fluid (SF) from patients with RA.
  
  
  METHODS
  Neutrophils' oxidative metabolism was measured by two types of CL. The first one, luminol-dependent CL (CL-lum), allows one to determine phagocytic activity and the level of ROI generated in a myeloperoxidase-dependent manner. The second method used was lucigenin-dependent CL (CL-luc), which monitors ROI production dependent on the NADPH oxidase enzyme complex located in the cell membranes of neutrophils and enables one to determine the scope of extracellular ROI emission.
  
  
  RESULTS
  Neutrophils stimulated by opsonized zymosan show a decrease in the level of CL-lum, proportional to the increasing concentration of both SF and serum collected from healthy donors. The observed effect of decreased CL-lum may, therefore, be dependent on the physical conditions (viscosity of fluids used). None of these experiments showed any effect of leptin on the level of CL-lum.
  
  
  CONCLUSIONS
  The present study showed that leptin does not affect the level of any of the CL types in inactive neutrophils incubated in normal serum, and it does not affect the level of oxidative activity in resting neutrophils incubated with SF. However, leptin influences extracellular ROI emission (measured by CL-luc). Leptin reduces extracellular emission of ROI, and this effect is dependent on concentration and duration of exposure to leptin.
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Rheumatology, Warsaw, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Gajewski
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Rheumatology, Warsaw, Poland.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Rzodkiewicz
authors.affiliation: Screening Test Department, Institute of Mother and Child, Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Gajewska
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Rheumatology, Warsaw, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Wojtecka-Łukasik
keywords: leptin; neutrophils; respiratory burst