title: Derivatization Strategy for the Comprehensive Characterization of Endogenous Fatty Aldehydes Using HPLC-Multiple Reaction Monitoring.
abstract: Fatty aldehydes are crucial substances that mediate a wide range of vital physiological functions, particularly lipid peroxidation. Fatty aldehydes such as acrolein and 4-hydroxynonenal (4-HNE) are considered potential biomarkers of myocardial ischemia and dementia, but analytical techniques for fatty aldehydes are lacking. In the present study, a comprehensive characterization strategy with high sensitivity and facility for fatty aldehydes based on derivatization and high-performance liquid chromatography-multiple reaction monitoring (HPLC-MRM) was developed. The fatty aldehydes of a biosample were derivatized using 2,4-bis(diethylamino)-6-hydrazino-1,3,5-triazine under mild and efficient reaction conditions at 37 °C for 15 min. The limit of detection (LOD) of the fatty aldehydes varied from 0.1 to 1 pg/mL, depending on the structures of these molecules. General MRM parameters were forged for the analysis of endogenous fatty aldehydes. "Heavy" derivatization reagents with 20 deuterium atoms were synthesized for both the discovery and comprehensive characterization of fatty aldehydes. More than 80 fatty aldehydes were detected in the biosamples. The new strategy was successfully implemented in global fatty aldehyde profiling of plasma and brain tissue of the bilateral common carotid artery (2VO) dementia rat model. Dozens of fatty aldehydes were significantly changed between the control and model groups. These findings further highlight the importance of endogenous fatty aldehydes.
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, PR China.
authors.forename: Cai
authors.initials: C
authors.lastname: Tie
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, PR China.
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, PR China.
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Zhang
mesh_terms: D000171:Acrolein / Q000032:analysis / Q000737:chemistry; D000447:Aldehydes / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D001921:Brain / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003704:Dementia / Q000473:pathology; D003903:Deuterium / Q000737:chemistry; D016002:Discriminant Analysis; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000032:analysis* / Q000097:blood; D057230:Limit of Detection; D008297:Male; D025341:Principal Component Analysis; D051381:Rats; D017208:Rats, Wistar; D014227:Triazines / Q000737:chemistry
chemical_list: D000447:Aldehydes; D015415:Biomarkers; D005227:Fatty Acids; D014227:Triazines; C001634:fatty aldehyde; D000171:Acrolein; D003903:Deuterium; C027576:4-hydroxy-2-nonenal