title: Data on expression of lipoxygenases-5 and -12 in the normal and acetaminophen-damaged liver.
abstract: Here we present additional data on the expression of lipoxygenases -5 and -12 in the normal and acetaminophen-damaged liver, which are associated with our manuscript recently published in Chemico-Biological Interactions on lipid metabolism and eicosanoid signaling pathways involved in acetaminophen-induced liver damage in a mouse model (http://dx.doi.org/10.1016/j.cbi.2015.10.019 [1]). It has been demonstrated that the expression of lipoxygenase-5 and leukotriene formation are increased in the livers of rats with carbon tetrachloride (CCl4)-induced cirrhosis (http://dx.doi.org/10.1053/gast.2000.17831 [2]). In addition, the lipoxygenase-12 is known to be expressed in the resident macrophage population of the liver (http://dx.doi.org/10.1016/S0014-5793(99)00396-8 [3]). Mice were injected with acetaminophen, and at 48 h their livers were processed for immunohistochemistry with anti-mouse lipoxygenase-5 and -12 antibodies. At the same time point, the RNA was also extracted from the liver to assess the expression of lipoxygenase-5 and -12 genes via qPCR analysis. Our results show that lipoxygenase-5 expression, but not that of lipoxygenase-12, changes significantly in the acetominophen-damaged liver.
authors.affiliation: Electron Microscopy Integrated Laboratory, National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath St., 400293 Cluj-Napoca, Romania; Molecular Biology and Biotechnology Department, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj 5-7 Clinicilor St., 400006 Napoca, Romania.
authors.forename: Maria
authors.initials: M
authors.lastname: Suciu
authors.affiliation: Regional Center for Transplant Immunology, County Clinical Emergency Hospital Timisoara, 10 Iosif Bulbuca Blvd., 300736 Timisoara, Romania.
authors.forename: Alexandra T
authors.initials: AT
authors.lastname: Gruia
authors.affiliation: Regional Center for Transplant Immunology, County Clinical Emergency Hospital Timisoara, 10 Iosif Bulbuca Blvd., 300736 Timisoara, Romania; Faculty of Animal Sciences and Biotechnologies, Banat׳s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania", Timisoara, Romania.
authors.forename: Dragos V
authors.initials: DV
authors.lastname: Nica
authors.affiliation: Department of Functional Sciences, University of Medicine and Pharmacy "Victor Babes", 2 Eftimie Murgu Sq., 300041 Timisoara, Romania.
authors.forename: Seyed M R
authors.initials: SM
authors.lastname: Azghadi
authors.affiliation: Department of Functional Sciences, University of Medicine and Pharmacy "Victor Babes", 2 Eftimie Murgu Sq., 300041 Timisoara, Romania.
authors.forename: Ani A
authors.initials: AA
authors.lastname: Mic
authors.affiliation: Department of Functional Sciences, University of Medicine and Pharmacy "Victor Babes", 2 Eftimie Murgu Sq., 300041 Timisoara, Romania.
authors.forename: Felix A
authors.initials: FA
authors.lastname: Mic