title: Cell cycle-regulated PLEIADE/AtMAP65-3 links membrane and microtubule dynamics during plant cytokinesis.
abstract: Cytokinesis, the partitioning of the cytoplasm following nuclear division, requires extensive coordination between cell cycle cues, membrane trafficking and microtubule dynamics. Plant cytokinesis occurs within a transient membrane compartment known as the cell plate, to which vesicles are delivered by a plant-specific microtubule array, the phragmoplast. While membrane proteins required for cytokinesis are known, how these are coordinated with microtubule dynamics and regulated by cell cycle cues remains unclear. Here, we document physical and genetic interactions between Transport Protein Particle II (TRAPPII) tethering factors and microtubule-associated proteins of the PLEIADE/AtMAP65 family. These interactions do not specifically affect the recruitment of either TRAPPII or MAP65 proteins to the cell plate or midzone. Rather, and based on single versus double mutant phenotypes, it appears that they are required to coordinate cytokinesis with the nuclear division cycle. As MAP65 family members are known to be targets of cell cycle-regulated kinases, our results provide a conceptual framework for how membrane and microtubule dynamics may be coordinated with each other and with the nuclear cycle during plant cytokinesis.
authors.affiliation: Botany, Technische Universität München, Freising, 85354, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Steiner
authors.affiliation: Botany, Technische Universität München, Freising, 85354, Germany.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Rybak
authors.affiliation: Plant Systems Biology, Technische Universität München, Freising, 85354, Germany.
authors.forename: Melina
authors.initials: M
authors.lastname: Altmann
authors.affiliation: School of Biosciences, University of Melbourne, Parkville, 3010, Victoria, Australia.|Max Planck Institute for Molecular Plant Physiology, Postdam, 14476, Germany.
authors.forename: Heather E
authors.identifier: 0000-0001-5569-5151
authors.initials: HE
authors.lastname: McFarlane
authors.affiliation: Chair of Proteomics and Bioanalytics, Technische Universität München, Freising, 85354, Germany.
authors.forename: Susan
authors.initials: S
authors.lastname: Klaeger
authors.affiliation: Botany, Technische Universität München, Freising, 85354, Germany.
authors.forename: Ngoc
authors.initials: N
authors.lastname: Nguyen
authors.affiliation: Department Biologie I, Ludwig-Maximillians Universität, Planegg-Martinsried, 82152, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Facher
authors.affiliation: School of Biosciences, University of Melbourne, Parkville, 3010, Victoria, Australia.|Max Planck Institute for Molecular Plant Physiology, Postdam, 14476, Germany.
authors.forename: Alexander
authors.identifier: 0000-0003-4091-1135
authors.initials: A
authors.lastname: Ivakov
authors.affiliation: Department Biologie I, Ludwig-Maximillians Universität, Planegg-Martinsried, 82152, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Wanner
authors.affiliation: Chair of Proteomics and Bioanalytics, Technische Universität München, Freising, 85354, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Kuster
authors.affiliation: School of Biosciences, University of Melbourne, Parkville, 3010, Victoria, Australia.|Max Planck Institute for Molecular Plant Physiology, Postdam, 14476, Germany.|School of Biosciences, ARC Centre of Excellence in Plant Cell Walls, University of Melbourne, Parkville, 3010, Victoria, Australia.
authors.forename: Staffan
authors.initials: S
authors.lastname: Persson
authors.affiliation: Plant Systems Biology, Technische Universität München, Freising, 85354, Germany.
authors.forename: Pascal
authors.initials: P
authors.lastname: Braun
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, 1190, Austria.
authors.forename: Marie-Theres
authors.initials: MT
authors.lastname: Hauser
authors.affiliation: Botany, Technische Universität München, Freising, 85354, Germany.
authors.forename: Farhah F
authors.initials: FF
authors.lastname: Assaad
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D048749:Cytokinesis / Q000235:genetics / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; C576219:MAP65-3 protein, Arabidopsis; D008869:Microtubule-Associated Proteins; C586781:TRS120 protein, Arabidopsis
keywords: 
  Arabidopsis thaliana
  ; 
  TRAPPII
  ; AtTRS120; CLUB/TRS130; MAP65-3/PLEAIDE; cell plate; cytokinesis; phragmoplast microtubules