title: Induction of Erythropoiesis by MHC-Mediated Cognate Interactions between B- and T-Cells.
abstract: We have previously shown that the expression of membrane burst-promoting activity (mBPA), an erythropoietic cytokine, by B-lymphocytes is augmented by the addition of allogeneic effector cells to the B-cells. Here, we have examined immune mechanisms involved in the induction/promotion of erythropoiesis as assessed by the capacity of autologous and allogeneic peripheral blood lymphocytes to augment burst-forming unit-erythroid (BFU-E) in normal human bone marrow cells in vitro. Preincubation of mBPA-expressing human B-cells with monoclonal antibodies to major histocompatibility complex (MHC) antigens, abrogated erythropoietic activity of both autologous and allogeneic lymphocytes, suggesting that MHC antigens play a role in regulating the expression of the erythroid growth factor. Inhibition of BFU-E proliferation was also evident when antibodies to MHC class-I or class-II antigens were added directly to marrow culture. Furthermore, addition of anti-CD4 antibody to the cultures of PBL and autologous target BM cells markedly reduced erythroid proliferation induced by PBL. By contrast, anti-CD8 and control (UPC-10) monoclonal antibodies had no effect. These results provide evidence that MHC-mediated cognate interactions between T- and B-lymphocytes may participate in the control of erythropoiesis, either directly or by modulating mBPA function.
authors.affiliation: a Departments of Medicine and Pathology , University of Connecticut Health Center , Farmington , CT and Department of Medicine , Bridgeport Hospital , Bridgeport , CT.
authors.forename: A
authors.initials: A
authors.lastname: Guha
authors.affiliation: a Departments of Medicine and Pathology , University of Connecticut Health Center , Farmington , CT and Department of Medicine , Bridgeport Hospital , Bridgeport , CT.
authors.forename: D P
authors.initials: DP
authors.lastname: Tuck
authors.affiliation: a Departments of Medicine and Pathology , University of Connecticut Health Center , Farmington , CT and Department of Medicine , Bridgeport Hospital , Bridgeport , CT.
authors.forename: R E
authors.initials: RE
authors.lastname: Cone
authors.affiliation: a Departments of Medicine and Pathology , University of Connecticut Health Center , Farmington , CT and Department of Medicine , Bridgeport Hospital , Bridgeport , CT.
authors.forename: N
authors.initials: N
authors.lastname: Dainiak
keywords: burst-promoting unit erythroid (BFU-E); erythropoiesis; major histocompatibility antigen (MHC)