title: Gender-Specific Toxicological Effects of Chronic Exposure to Pure Microcystin-LR or Complex Microcystis aeruginosa Extracts on Adult Medaka Fish.
abstract: Cyanobacterial blooms often occur in freshwater lakes and constitute a potential health risk to human populations, as well as to other organisms. However, their overall and specific implications for the health of aquatic organisms that are chronically and environmentally exposed to cyanobacteria producing hepatotoxins, such as microcystins (MCs), together with other bioactive compounds have still not been clearly established and remain difficult to assess. The medaka fish was chosen as the experimental aquatic model for studying the cellular and molecular toxicological effects on the liver after chronic exposures (28 days) to environmentally relevant concentrations of pure MC-LR, complex extracts of MC producing or nonproducing cyanobacterial biomasses, and of a Microcystis aeruginosa natural bloom. Our results showed a higher susceptibility of females to the different treatments compared to males at both the cellular and the molecular levels. Although hepatocyte lysis increased with MC-containing treatments, lysis always appeared more severe in the liver of females compare to males, and the glycogen cellular reserves also appeared to decrease more in the liver of females compared to those in the males. Proteomic investigations reveal divergent responses between males and females exposed to all treatments, especially for proteins involved in metabolic and homeostasis processes. Our observations also highlighted the dysregulation of proteins involved in oogenesis in female livers. These results suggest that fish populations exposed to cyanobacteria blooms may potentially face several ecotoxicological issues.
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de Communication et Adaptation des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle , CP 39, 12 Rue Buffon, 75005 Paris, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Le Manach
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de Communication et Adaptation des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle , CP 39, 12 Rue Buffon, 75005 Paris, France.
authors.forename: Nour
authors.initials: N
authors.lastname: Khenfech
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de Communication et Adaptation des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle , CP 39, 12 Rue Buffon, 75005 Paris, France.|Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est , BioPôle Alfort, 94700 Maison-Alfort, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Huet
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de Communication et Adaptation des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle , CP 39, 12 Rue Buffon, 75005 Paris, France.
authors.forename: Qin
authors.initials: Q
authors.lastname: Qiao
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de Communication et Adaptation des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle , CP 39, 12 Rue Buffon, 75005 Paris, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Duval
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de Communication et Adaptation des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle , CP 39, 12 Rue Buffon, 75005 Paris, France.
authors.forename: Arul
authors.initials: A
authors.lastname: Marie
authors.affiliation: Institut de Biologie Paris Seine/FR 3631, Plateforme Spectrométrie de masse et Protéomique, Sorbonne Universités, Université Pierre et Marie Curie , 75005 Paris, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Bolbach
authors.affiliation: Institut de Biologie Paris Seine/FR 3631, Plateforme Spectrométrie de masse et Protéomique, Sorbonne Universités, Université Pierre et Marie Curie , 75005 Paris, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Clodic
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de Communication et Adaptation des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle , CP 39, 12 Rue Buffon, 75005 Paris, France.
authors.forename: Chakib
authors.initials: C
authors.lastname: Djediat
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de Communication et Adaptation des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle , CP 39, 12 Rue Buffon, 75005 Paris, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Bernard
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de Communication et Adaptation des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle , CP 39, 12 Rue Buffon, 75005 Paris, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Edery
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de Communication et Adaptation des Micro-organismes, Sorbonne Universités, Muséum National d'Histoire Naturelle , CP 39, 12 Rue Buffon, 75005 Paris, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Marie
mesh_terms: D000818:Animals; D000458:Cyanobacteria / Q000378:metabolism; D060106:Lakes; D052998:Microcystins / Q000378:metabolism; D046931:Microcystis / Q000378:metabolism*; D009990:Oryzias / Q000378:metabolism*; D040901:Proteomics
chemical_list: D052998:Microcystins