title: Immobilization of Lipase by Adsorption Onto Magnetic Nanoparticles in Organic Solvents.
abstract: In order to improve the performance of lipase in organic solvents, a simple immobilization method was developed by adsorption of lipase onto Fe₃O₄@ SiO₂magnetic nanoparticles in organic solvent. Among the solvents tested, toluene was found to be the most effective solvent for the immobilization. A maximum immobilization yield of 97% and relative activity of 124% were achieved in toluene at 30 °C. The optimal temperature, enzyme loading and water activity were 30 °C, 1.25 mg/mg support and 0.48 aw, respectively. The residual activity of immobilized lipase was 67% after 10 cycles of use. The advantages of the immobilized lipase including easy recovery, high stability, and enhanced activity of immobilized lipase in organic solvents show potential industrial applications in anhydrous solvents.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Qing-Lan
authors.initials: QL
authors.lastname: Tao
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Jiang
authors.forename: Cai-Hong
authors.initials: CH
authors.lastname: Liu
authors.forename: Sha
authors.initials: S
authors.lastname: Zeng
authors.forename: Ye-Wang
authors.initials: YW
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D001234:Aspergillus niger / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D014050:Toluene / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D005656:Fungal Proteins; D058185:Magnetite Nanoparticles; D012997:Solvents; C000499:ferric oxide; D014050:Toluene; D012822:Silicon Dioxide; D008049:Lipase