title: Comparative proteomic analysis in Aphis glycines Mutsumura under lambda-cyhalothrin insecticide stress.
abstract: Lambda-cyhalothrin is now widely used in China to control the soybean aphid Aphis glycines. To dissect the resistance mechanism, a laboratory-selected resistant soybean aphid strain (CRR) was established with a 43.42-fold resistance ratio to λ-cyhalothrin than the susceptible strain (CSS) in adult aphids. In this study, a comparative proteomic analysis between the CRR and CSS strains revealed important differences between the susceptible and resistant strains of soybean aphids for λ-cyhalothrin. Approximately 493 protein spots were detected in two-dimensional polyacrylamide gel electrophoresis (2-DE). Thirty-six protein spots displayed differential expression of >2-fold in the CRR strain compared to the CSS strain. Out of these 36 protein spots, 21 had elevated and 15 had decreased expression. Twenty-four differentially expressed proteins were identified by MALDI TOF MS/MS and categorized into the functional groups cytoskeleton-related protein, carbohydrate and energy metabolism, protein folding, antioxidant system, and nucleotide and amino acid metabolism. Function analysis showed that cytoskeleton-related proteins and energy metabolism proteins have been associated with the λ-cyhalothrin resistance of A. glycines. The differential expression of λ-cyhalothrin responsive proteins reflected the overall change in cellular structure and metabolism after insecticide treatment in aphids. In summary, our studies improve understanding of the molecular mechanism resistance of soybean aphid to lambda-cyhalothrin, which will facilitate the development of rational approaches to improve the management of this pest and to improve the yield of soybean.
authors.affiliation: College of Plant Science, Jilin University, ChangChun 130062, PR China; College of Agronomy, Jilin Agricultural University, ChangChun 130118, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Bi
authors.affiliation: College of Plant Science, Jilin University, ChangChun 130062, PR China.
authors.forename: Yiou
authors.initials: Y
authors.lastname: Pan
authors.affiliation: College of Plant Science, Jilin University, ChangChun 130062, PR China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Shang
authors.affiliation: College of Plant Science, Jilin University, ChangChun 130062, PR China.
authors.forename: Tianfei
authors.initials: T
authors.lastname: Peng
authors.affiliation: College of Plant Science, Jilin University, ChangChun 130062, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yang
authors.affiliation: College of Plant Science, Jilin University, ChangChun 130062, PR China.
authors.forename: Shang
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Plant Science, Jilin University, ChangChun 130062, PR China.
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Xin
authors.affiliation: College of Plant Science, Jilin University, ChangChun 130062, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Plant Science, Jilin University, ChangChun 130062, PR China. Electronic address: jhxi1965@jlu.edu.cn.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Xi
mesh_terms: D000818:Animals; D001042:Aphids / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D007306:Insecticides / Q000494:pharmacology; D009570:Nitriles / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D011722:Pyrethrins / Q000494:pharmacology*; D013025:Glycine max / Q000187:drug effects / Q000378:metabolism* / Q000469:parasitology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry
chemical_list: D007306:Insecticides; D009570:Nitriles; D020543:Proteome; D011722:Pyrethrins; C037304:cyhalothrin
keywords: Aphis glycines Matsumura; Insecticide resistance; Lambda-cyhalothrin; Proteomics