title: Melanin-Like Nanoquencher on Graphitic Carbon Nitride Nanosheets for Tyrosinase Activity and Inhibitor Assay.
abstract: Graphitic C3N4 (g-C3N4) nanosheets are a type of emerging graphene-like carbon-based nanomaterials with high fluorescence and large specific surface areas that hold great potential for biosensor applications. However, current g-C3N4 based biosensors have prevailingly been limited to coordination with metal ions, and it is of great significance to develop new designs for g-C3N4 nanosheets based biosensors toward biomarkers of general interest. We report the development of a novel g-C3N4 nanosheet-based nanosensor strategy for highly sensitive, single-step and label-free detection of tyrosinase (TYR) activity and its inhibitor. This strategy relies on the catalytic oxidation of tyrosine by TYR into melanin-like polymers, which form a nanoassembly on the g-C3N4 nanosheets and quench their fluorescence. This strategy was demonstrated to provide excellent selectivity and superior sensitivity and to enable rapid screening for TYR inhibitors. Therefore, the developed approach might create a useful platform for diagnostics and drugs screening for TYR-based diseases including melanoma cancer.
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082 P. R. China.
authors.forename: Jin-Wen
authors.initials: JW
authors.lastname: Liu
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082 P. R. China.
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Wang
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082 P. R. China.
authors.forename: Liu
authors.initials: L
authors.lastname: Xu
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082 P. R. China.
authors.forename: Lu-Ying
authors.initials: LY
authors.lastname: Duan
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082 P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082 P. R. China.
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082 P. R. China.
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Jiang
mesh_terms: D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D008543:Melanins / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D009570:Nitriles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D004791:Enzyme Inhibitors; D008543:Melanins; D009570:Nitriles; D011108:Polymers; C011206:cyanogen; D014442:Monophenol Monooxygenase