title: Isolation and Colony Formation of Murine Bone and Bone Marrow Cells.
abstract: Adult homeostasis is dependent on normal Wt1 expression. Loss of Wt1 expression in adult mice causes rapid loss of the mesenchymal tissues, fat and bone, amongst other phenotypes. Bone and bone marrow mesenchymal stromal cells can be studied by cell isolation and expansion. The stemness of these cells can then be characterized by carrying out a colony-forming unit-fibroblast assay and observing clonogenic capabilities.
authors.affiliation: MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, EH4 2XU, UK.
authors.forename: Sophie
authors.initials: S
authors.lastname: McHaffie
authors.affiliation: MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, EH4 2XU, UK. You-Ying.Chau@ed.ac.uk.|British Heart Foundation Centrefor Cardiovascular Science, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK. You-Ying.Chau@ed.ac.uk.
authors.forename: You-Ying
authors.initials: YY
authors.lastname: Chau
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D001842:Bone and Bones / Q000166:cytology*; D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay / Q000379:methods*; D005347:Fibroblasts; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D013234:Stem Cells / Q000166:cytology
keywords: Bone; Bone marrow; Colony-forming unit fibroblast; Cre; Mesenchymal; Stem cell culture