title: Di(2-ethylhexyl) phthalate induces apoptosis through mitochondrial pathway in GC-2spd cells.
abstract: Di(2-ethylhexyl) phthalate (DEHP), a plasticizer of synthetic polymers, is a well-known endocrine disrupting chemical (EDC) and reproductive toxicant. Addressing the unclear mechanism of DEHP-induced reproductive dysfunction, this study used GC-2spd cells to investigate the molecular mechanism involved in the DEHP-induced toxicity in the male reproductive system. The results indicated that the apoptotic cell death was significantly induced by DEHP exposure over 100 μM. Furthermore, DEHP treatment could induce oxidative stress in GC-2spd cells involving in the decrease of superoxide dismutase (SOD) activity (200 μM) and glutathione peroxidase (GSH-Px) activity (50 and 100 μM). In addition, DEHP induction also caused the elevated ratios of Bax/Bcl-2, release of cytochrome c and decomposition of procaspase-3 and procaspase-9 in GC-2spd cells. Taken together, our work provided the evidence that DEHP exposure might induce apoptosis of GC-2spd cells via mitochondria pathway mediated by oxidative stress. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1055-1064, 2017.
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Medical College, Wuhan University of Science and Technology, 947 Heping Avenue, Wuhan, 430081, People's Republic of China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Fu
authors.affiliation: Department of Non-communicable chronic disease prevention and control, Wuhan Centers for Disease Prevention and Control, 24 Jianghan N.Road, Wuhan, 430015, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Dai
authors.affiliation: Lancaster Environment Centre, Lancaster University, Lancaster, LA1 4YQ, United Kingdom.
authors.forename: Dayi
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Medical College, Wuhan University of Science and Technology, 947 Heping Avenue, Wuhan, 430081, People's Republic of China.
authors.forename: Lishan
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Medical College, Wuhan University of Science and Technology, 947 Heping Avenue, Wuhan, 430081, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Medical College, Wuhan University of Science and Technology, 947 Heping Avenue, Wuhan, 430081, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Medical College, Wuhan University of Science and Technology, 947 Heping Avenue, Wuhan, 430081, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Huazhong University of Science and Technology, Tongji Medical College, 13 Hangkong Road, Wuhan, Hubei, 430030, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Duan
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Huazhong University of Science and Technology, Tongji Medical College, 13 Hangkong Road, Wuhan, Hubei, 430030, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Quan
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Medical College, Wuhan University of Science and Technology, 947 Heping Avenue, Wuhan, 430081, People's Republic of China.|Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, Virginia, 23298.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Medical College, Wuhan University of Science and Technology, 947 Heping Avenue, Wuhan, 430081, People's Republic of China.
authors.forename: Shizhen
authors.initials: S
authors.lastname: Song
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Medical College, Wuhan University of Science and Technology, 947 Heping Avenue, Wuhan, 430081, People's Republic of China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002478:Cells, Cultured; D004051:Diethylhexyl Phthalate / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity*; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010968:Plasticizers / Q000633:toxicity; D015398:Signal Transduction / Q000187:drug effects; D013090:Spermatocytes / Q000187:drug effects* / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D010968:Plasticizers; D004051:Diethylhexyl Phthalate; D053148:Caspase 3; D053453:Caspase 9
keywords: Di(2-ethylhexyl) phthalate (DEHP); GC-2spd cells; apoptosis; mitochondrial pathway; oxidative stress