title: Influence of oxidized lipids on palmitoyl-oleoyl-phosphatidylcholine organization, contribution of Langmuir monolayers and Langmuir-Blodgett films.
abstract: In this work, we studied the interaction of two oxidized lipids, PoxnoPC and PazePC, with POPC phospholipid. Mean molecular areas obtained from (π-A) isotherms of mixed PoxnoPC-POPC and PazePC-POPC monolayers revealed different behaviors of these two oxidized lipids: the presence of PoxnoPC in the monolayers induces their expansion, mean molecular areas being higher than those expected in the case of ideal mixtures. PazePC-POPC behave on the whole ideally. This difference can be explained by a different conformation of oxidized lipids. Moreover the carboxylic function of PazePC is protonated under our experimental conditions, as shown by (π-A) isotherms of PazePC at different pH values. Both oxidized lipids induce also an increase of the monolayer elasticity, PoxnoPC being slightly more efficient than PazePC. These monolayers were transferred from the air-water interface onto mica supports for a study by AFM. AFM images are on the whole homogenous, suggesting the presence of only one lipid phase in both cases. However, in the case of PazePC-POPC monolayers, AFM images show also the presence of areas thicker of 7nm to 10nm than the surrounding lipid phase, probably due to the local formation of multilayer systems induced by compression.
authors.affiliation: Laboratoire Ondes et Matière d'Aquitaine (LOMA), UMR CNRS 5798, Université de Bordeaux, 351 cours de la libération, 33405 Talence Cedex, France. Electronic address: christine.grauby-heywang@u-bordeaux.fr.
authors.forename: Christine
authors.initials: C
authors.lastname: Grauby-Heywang
authors.affiliation: Laboratoire Ondes et Matière d'Aquitaine (LOMA), UMR CNRS 5798, Université de Bordeaux, 351 cours de la libération, 33405 Talence Cedex, France. Electronic address: fabien.morote@u-bordeaux.fr.
authors.forename: Fabien
authors.initials: F
authors.lastname: Moroté
authors.affiliation: Laboratoire Ondes et Matière d'Aquitaine (LOMA), UMR CNRS 5798, Université de Bordeaux, 351 cours de la libération, 33405 Talence Cedex, France. Electronic address: marion.mathelie-guinlet@u-bordeaux.fr.
authors.forename: Marion
authors.initials: M
authors.lastname: Mathelié-Guinlet
authors.affiliation: Cellule de transfert NanoPhyNov, Université de Bordeaux, 351 cours de la libération, Talence Cedex 33405, France. Electronic address: ibtissem.gammoudi@u-bordeaux.fr.
authors.forename: Ibtissem
authors.initials: I
authors.lastname: Gammoudi
authors.affiliation: Laboratoire Ondes et Matière d'Aquitaine (LOMA), UMR CNRS 5798, Université de Bordeaux, 351 cours de la libération, 33405 Talence Cedex, France. Electronic address: rokhaya.faye@ihu-liryc.fr.
authors.forename: Ndeye Rokhaya
authors.initials: NR
authors.lastname: Faye
authors.affiliation: Laboratoire Ondes et Matière d'Aquitaine (LOMA), UMR CNRS 5798, Université de Bordeaux, 351 cours de la libération, 33405 Talence Cedex, France. Electronic address: touria.cohen-bouhacina@u-bordeaux.fr.
authors.forename: Touria
authors.initials: T
authors.lastname: Cohen-Bouhacina
mesh_terms: D018625:Microscopy, Atomic Force; D008968:Molecular Conformation; D010084:Oxidation-Reduction; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D010713:Phosphatidylcholines; C028694:1-palmitoyl-2-oleoylphosphatidylcholine
keywords: Atomic force microscopy; Langmuir monolayer; Langmuir–Blodgett film; Oxidized lipid; Surface pressure measurements