title: Eupatorium lindleyanum DC. flavonoids fraction attenuates lipopolysaccharide-induced acute lung injury in mice.
abstract: Eupatorium lindleyanum DC., "Ye-Ma-Zhui" called by local residents in China, showed anti-inflammatory activity and is used to treat tracheitis. We had isolated and identified the flavonoids, diterpenoids and sesquiterpenes compounds from the herb. In the present study, we evaluated the protective effects of the flavonoids fraction of E. lindleyanum (EUP-FLA) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice and the possible underlying mechanisms of action. EUP-FLA could significantly decrease lung wet-to-dry weight (W/D) ratio, nitric oxide (NO) and protein concentration in BALF, lower myeloperoxidase (MPO) activity, increase superoxide dismutase (SOD) activity and down-regulate the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β). Additionally, EUP-FLA attenuated lung histopathological changes and significantly reduced complement deposition with decreasing the levels of Complement 3 (C3) and Complement 3c (C3c) in serum. These results demonstrated that EUP-FLA may attenuate LPS-induced ALI via reducing productions of pro-inflammatory mediators, decreasing the level of complement and affecting the NO, SOD and MPO activity.
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China.
authors.forename: Chunjun
authors.initials: C
authors.lastname: Chu
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China.
authors.forename: Shi
authors.initials: S
authors.lastname: Yao
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China.
authors.forename: Jinglei
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Wei
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Xia
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, PR China.
authors.forename: Daofeng
authors.initials: D
authors.lastname: Chen
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China. Electronic address: jianzhang@suda.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy*; D000818:Animals; D015932:Complement C3c / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D036602:Eupatorium / Q000276:immunology*; D005419:Flavonoids / Q000627:therapeutic use*; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016207:Cytokines; D005419:Flavonoids; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide; D015932:Complement C3c; D009195:Peroxidase; D013482:Superoxide Dismutase
keywords: Acute lung injury; Complement; Eupatorium lindleyanum DC.; Flavonoids fraction; Lipopolysaccharide; Pro-inflammatory mediators