title: Association of the microsatellite (GT)n repeat polymorphisms of the HO-1 gene promoter and corresponding serum levels with the risk of laryngeal squamous cell carcinoma.
abstract: CONCLUSIONS
  Long (GT)n repeat polymorphisms in the heme oxygenase-1 (HO-1) gene promoter and decreased serum HO-1 levels are associated with a higher susceptibility to laryngeal squamous cell carcinoma (LSCC).
  
  
  OBJECTIVE
  In this case-control study, the association of HO-1 microsatellite (GT)n repeat polymorphisms and serum levels with the risk of LSCC was investigated.
  
  
  METHODS
  A total of 142 LSCC patients, 54 vocal leukoplakia patients and 98 healthy controls, were examined for (GT)n polymorphisms by sequencing, and the serum HO-1 levels were detected in a sub-set from participants above by ELISA.
  
  
  RESULTS
  Compared with the controls, the LSCC group had significantly higher frequencies of L-allele (> 29 repeats) and L-allele carriers (p < 0.001, OR = 2.037 and p = 0.005, OR = 2.152, respectively). The frequencies of lymph node metastasis and of moderate or poor differentiation were significantly higher in L-allele carriers compared to non-L-allele carriers (p < 0.05). Significantly lower serum HO-1 levels were detected in LSCC patients (p < 0.001), and patients with lower serum HO-1 levels had more advanced cancer stage and a higher lymph node metastasis rate (p < 0.05). Furthermore, the L-allele carriers had lower serum HO-1 concentrations compared with the non-L-allele carriers (p = 0.019).
authors.affiliation: a Department of Otolaryngology, Shanghai Key Clinical Disciplines of otorhinolaryngology , Eye Ear Nose & Throat Hospital, Fudan University , Shanghai , PR China ;
authors.forename: Di
authors.initials: D
authors.lastname: Tang
authors.affiliation: a Department of Otolaryngology, Shanghai Key Clinical Disciplines of otorhinolaryngology , Eye Ear Nose & Throat Hospital, Fudan University , Shanghai , PR China ;
authors.forename: Wei-Jing
authors.initials: WJ
authors.lastname: Tang
authors.affiliation: a Department of Otolaryngology, Shanghai Key Clinical Disciplines of otorhinolaryngology , Eye Ear Nose & Throat Hospital, Fudan University , Shanghai , PR China ;
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Shi
authors.affiliation: b Department of Otolaryngology , Pudong Hospital, Fudan University , PR China ;
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Li
authors.affiliation: b Department of Otolaryngology , Pudong Hospital, Fudan University , PR China ;
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: c Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine , Shanghai , PR China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Lu
authors.affiliation: a Department of Otolaryngology, Shanghai Key Clinical Disciplines of otorhinolaryngology , Eye Ear Nose & Throat Hospital, Fudan University , Shanghai , PR China ;|b Department of Otolaryngology , Pudong Hospital, Fudan University , PR China ;
authors.forename: Lei
authors.initials: L
authors.lastname: Tao
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005931:Glottis / Q000473:pathology; D051547:Heme Oxygenase-1 / Q000097:blood / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D007971:Leukoplakia / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1
keywords: Heme oxygenase-1; laryngeal squamous cell carcinoma; polymorphism; serum; vocal cord leukoplakia