title: Lack of TMEM27 expression is associated with postoperative progression of clinically localized conventional renal cell carcinoma.
abstract: OBJECTIVE
  In spite of early detection of conventional renal cell carcinoma (RCC) by widespread use of abdominal imaging, approximately 10-15 % of patients will die due to disease. The aim of this study was to identify new biomarkers predicting the postoperative progression of conventional RCC.
  
  
  METHODS
  Tissue multiarrays (TMA) of conventional RCC from a cohort of 486 patients were analysed by immunohistochemistry for expression of the transmembrane protein TMEM27, which was identified as a candidate biomarker by Affymetrix U133 Plus 2.0 array. Univariate and multivariate Cox regression models were addressed to assess cancer-specific survival in association with clinicopathological variables and TMEM27 expression. Cancer-specific survival time was estimated with Kaplan-Meier analysis, and the comparison of survival curves was made with the log-rank test.
  
  
  RESULTS
  The Kaplan-Meier survival analysis indicated a poor disease-specific survival rates for tumours without TMEM27 staining. Univariate analysis revealed an association of patient survival with T stadium, grade, stage and size of tumour and TMEM27 expression in all cases as well as in the cohort of patients with postoperative tumour progression. In multivariate analysis, only T stadium and TMEM27 staining showed a significant association with postoperative cancer-specific death (p < 0.001).
  
  
  CONCLUSIONS
  Lack of expression of the TMEM27 in conventional RCC defines a group of patients at high risk for cancer-related death.
authors.affiliation: Department of Urology, Medical School, University of Pecs, Munkacsy M. u. 2, Pecs, 7621, Hungary.
authors.forename: Andras
authors.initials: A
authors.lastname: Javorhazy
authors.affiliation: Institute of Bioanalysis, Medical School, University of Pecs, Pecs, Hungary.
authors.forename: Nelli
authors.initials: N
authors.lastname: Farkas
authors.affiliation: Department of Urology, Medical School, University of Pecs, Munkacsy M. u. 2, Pecs, 7621, Hungary.
authors.forename: Tamas
authors.initials: T
authors.lastname: Beothe
authors.affiliation: Department of Urology, Medical School, University of Pecs, Munkacsy M. u. 2, Pecs, 7621, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Pusztai
authors.affiliation: Department of Urology, Medical School, University of Pecs, Munkacsy M. u. 2, Pecs, 7621, Hungary.
authors.forename: Arpad
authors.initials: A
authors.lastname: Szanto
authors.affiliation: Department of Urology, Medical School, University of Pecs, Munkacsy M. u. 2, Pecs, 7621, Hungary. G.Kovacs@gmx.de.
authors.forename: Gyula
authors.initials: G
authors.lastname: Kovacs
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D053208:Kaplan-Meier Estimate; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009392:Nephrectomy; D020411:Oligonucleotide Array Sequence Analysis; D011184:Postoperative Period; D011379:Prognosis; D016019:Survival Analysis; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; C429534:CLTRN protein, human; D008562:Membrane Glycoproteins
keywords: Immunohistochemistry; Renal cell carcinoma; Survival; TMEM27