title: Preparation and Evaluation of Oxaliplatin Thermosensitive Liposomes with Rapid Release and High Stability.
abstract: Oxaliplatin (OXP) was reported to show low anti-tumor activity when used alone and to display side effects; this low activity was attributed to high partitioning to erythrocytes and low accumulation in tumors. Thermosensitive liposomes (TSL) were considered able to specifically deliver drugs to heated tumors and to resolve the OXP distribution problem. Regretfully, TSL encapsulating doxorubicin did not demonstrate significant improvement in progression-free survival. Drug release below 41°C and significant leakage were considered major reasons for the failure. The purpose of this study was to acquire OXP TSL with rapid release at the triggered temperature and high stability at body temperature and at storage temperatures. A small quantity of poloxamer 188 was introduced into the TSL formulation to stabilize the encapsulated drug. It was shown that the addition of poloxamer 188 had no influence on the TSL characteristics. More than 90% of OXP was released within 10 min at 42°C, and less than 15% was released within 60 min at temperatures below 39°C. TSL were stable at 37°C for 96 h and at 4°C for 6 months. The anti-tumor activity of TSL at the dose of 2.5 mg/kg was certified to be equal to those of OXP injection and non-thermosensitive liposomes (NTSL) at the dose of 5 mg/kg, and significant improvement of tumor inhibition was observed in TSL compared with injection and NTSL at the same dose. It was also shown from the histological transmutation of tumors that TSL had stronger anti-tumor activity. Therefore, it could be concluded that TSL composed of a proper amount of poloxamer had rapid release and high stability, and OXP TSL would be anticipated to exert prominent anti-tumor activity in the clinic.
authors.affiliation: Pharmacy Department, Fu Xing Hospital Capital Medical University, Beijing, China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Zeng
authors.affiliation: Pharmaceutical Department, Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Fanglin
authors.initials: F
authors.lastname: Yu
authors.affiliation: Pharmaceutical Department, Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Pharmaceutical Department, Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Pharmaceutical Department, Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Pharmaceutical Department, Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Pharmaceutical Department, Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Shiqing
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Pharmacy Department, No. 261 Hospital of PLA, Beijing, China.
authors.forename: Zhenbo
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Pharmaceutical Department, Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Li
authors.affiliation: Pharmaceutical Department, Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Li
authors.affiliation: Pharmaceutical Department, Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Mei
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage; D016503:Drug Delivery Systems / Q000379:methods*; D065546:Drug Liberation; D008081:Liposomes; D008175:Lung Neoplasms / Q000188:drug therapy; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009944:Organoplatinum Compounds / Q000008:administration & dosage*; D000077150:Oxaliplatin
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D004317:Doxorubicin