title: [Correlation between the genetic polymorphisms of apolipoprotein M with the susceptibility to rheumatic diseases of Chinese Han populastion in Lanzhou].
abstract: Objective To investigate the relationship between the genetic polymorphisms of apolipoprotein M (ApoM) and the susceptibility to rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and ankylosing spondylitis (AS) among Chinese Han population in Lanzhou. Methods Primers for the two single nucleotide polymorphism (SNP) sites (rs805296 and rs805297) in ApoM gene were designed and their genotyping methods of polymerase chain reaction-high resolution melting (PCR-HRM) assay were established. Case-control studies were performed among the 599 cases of RA, 194 cases of SLE, 179 cases of AS and 273 matched healthy controls to analyze the correlations between the two SNPs and the susceptibility to rheumatic diseases. Results The genotype frequencies of rs805296 were AA 87.0%, AG 12.7%, GG 0.3% in RA cases, AA 84.5%, AG 15.0%, GG 0.5% in SLE cases, AA 91.6%, AG 7.3%, GG 1.1% in AS cases, AA 85.0%, AG 15.0%, GG 0% in healthy controls. The ones of rs805297 were GG 38.2%, GT 51.8%, TT 10.0% in RA cases, GG 44.3%, GT 45.4%, TT 10.3% in SLE cases, GG 37.4%, GT 47.5%, TT 15.1% in AS cases, GG 40.7%, GT 46.1%, TT 13.2% in healthy controls. Statistical analyses showed that only the genotype distribution of rs805296 was significantly different between the AS cases and the healthy controls. Under the dominant model, the G allele carriers of rs805296 (AG heterozygote and GG homozygote) were found to significantly decrease the risk for AS development. Conclusion The established PCR-HRM genotyping assays in the present study can successfully achieve the molecular diagnosis of the two SNPs sites (rs805296 and rs805297) from clinical samples, and the study found a significant association between the SNP of rs805296 and the susceptibility to AS among Chinese Han population in Lanzhou.
authors.affiliation: Center for Laboratory Medicine, Lanzhou University Second Hospital, Lanzhou 730030, China.
authors.forename: Meiyong
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Xinling
authors.initials: X
authors.lastname: Guo
authors.affiliation: Center for Laboratory Medicine, Lanzhou University Second Hospital, Lanzhou 730030, China.
authors.forename: Qiannan
authors.initials: Q
authors.lastname: Li
authors.affiliation: Center for Laboratory Medicine, Lanzhou University Second Hospital, Lanzhou 730030; Gansu Provincial Key Laboratory of Digestive System Tumors, Lanzhou 730030, China. *Corresponding author, E-mail: youchg@lzu.edu.cn.
authors.forename: Chongge
authors.initials: C
authors.lastname: You
mesh_terms: D000328:Adult; D000483:Alleles; D001053:Apolipoproteins / Q000235:genetics*; D000075243:Apolipoproteins M; D001172:Arthritis, Rheumatoid / Q000208:ethnology / Q000235:genetics; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics*; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D054834:Lipocalins / Q000235:genetics*; D008180:Lupus Erythematosus, Systemic / Q000208:ethnology / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012216:Rheumatic Diseases / Q000208:ethnology / Q000235:genetics*; D013167:Spondylitis, Ankylosing / Q000208:ethnology / Q000235:genetics
chemical_list: C400523:APOM protein, human; D001053:Apolipoproteins; D000075243:Apolipoproteins M; D054834:Lipocalins