title: A conveniently prepared and hypersensitized small molecular fluorescent probe: Rapidly detecting free zinc ion in HepG2 cells and Arabidopsis.
abstract: In this paper, we reported a conveniently prepared fluorescent probe for zinc ions detection, which constructed by the condensation reaction between p-(benzothiazolyl)aniline with 4, 4- diethylaminesalicylaldehyde. The sensing ability of the probe toward zinc ions in vitro was tested by a series of UV-Vis and fluorescence spectroscopy studies, which showed that the probe possessed high sensitivity with a detection limit of 5.8nM and a rapid response time of 10s. We also carried out fluorescent bio-imaging of the probe for zinc ions in human liver hepatocellular carcinoma cells (HepG2), which showed that the probe could be utilized to detect the intracellular endogenous zinc ions visually without introducing external zinc sources. Meanwhile, co-staining experiment with organelle selective trackers was performed to illustrate that the probe could locate at endoplasmic reticulum. Finally, we successfully used it as a zinc ion developer in plant tissue, which clearly demonstrated the distribution of zinc ions in the growth stage of plant tissue.
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China; Department of Applied Chemistry, School of Science, Anhui Agricultural University, Hefei 230036, PR China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Gan
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Sun
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, PR China.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Tian
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, PR China.
authors.forename: Baowei
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China.
authors.forename: Jieying
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Tian
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China. Electronic address: zhpzhp@263.net.
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhou
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D056945:Hep G2 Cells; D006801:Humans; D007477:Ions / Q000032:analysis; D057054:Molecular Imaging / Q000379:methods*; D008970:Molecular Weight; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D015032:Zinc / Q000032:analysis / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D007477:Ions; D015032:Zinc
keywords: Bio-imaging application; Fluorescence probes; High sensitivity; Rapid-response ability; Zinc ion