title: Uniform and stable hydrogel-filled liposome-analogous vesicles with a thin elastomer shell layer.
abstract: This study introduces a new type of uniform liposome-analogous vesicle with a highly stable shell structure in which water-in-oil-in-water double emulsion drops fabricated in a capillary-based microfluidic device are used as templates. The vesicles developed in this work consist of a poly(ethylene glycol) hydrogel core surrounded by a polyurethane (PU) film between 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) layers. Subjecting the double emulsion templates to UV irradiation leads to the formation of a PU elastomer film between the DPPC layers. The presence of a thin PU film sandwiched between the DPPC layers is confirmed by confocal laser microscopy. The thicknesses of the PU films are measured to be approximately ∼4μm. Further study reveals the incorporation of the PU film between the DPPC layers remarkably improves the shell impermeability. Our vesicle system is expected to be useful for regulating the permeation of small molecules through lipid-based vesicular films.
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 15588, Republic of Korea.
authors.forename: Mintae
authors.initials: M
authors.lastname: Seo
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 15588, Republic of Korea.
authors.forename: Aram
authors.initials: A
authors.lastname: Byun
authors.affiliation: AMOREPACIFIC Co., R&D Center, Yongin 17074, Republic of Korea.
authors.forename: Jongwon
authors.initials: J
authors.lastname: Shim
authors.affiliation: Shinsegae International Co., Ltd., Seoul 06015, Republic of Korea.
authors.forename: Hong Sung
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Department of Applied Chemistry, Hanyang University, Ansan 15588, Republic of Korea. Electronic address: yblee@hanyang.ac.kr.
authors.forename: Youngbok
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 15588, Republic of Korea; Department of Applied Chemistry, Hanyang University, Ansan 15588, Republic of Korea. Electronic address: kjwoong@hanyang.ac.kr.
authors.forename: Jin Woong
authors.initials: JW
authors.lastname: Kim
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D020724:Elastomers / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D044085:Microfluidics / Q000379:methods; D011140:Polyurethanes / Q000737:chemistry
chemical_list: D020724:Elastomers; D008081:Liposomes; D011140:Polyurethanes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D015060:1,2-Dipalmitoylphosphatidylcholine; C081581:colfosceril palmitate
keywords: Elastomer film; Impermeability; Microfluidics; Vesicle