title: Composition and Biological Activity of Volatile Oil from Salviajudaica and S. multicaulis from Jordan.
abstract: The aim of this work was to determine the composition of the hydro-distilled essential oil of Salvia judaica Boiss. and S. multicaulis Vahl. (Lamiaceae) from Jordan by GC and GC-MS and to report the actual composition of their fresh leaves and flowers using SPME (Solid Phase Micro-Extraction).Their dual alpha-amylase/alpha glucosidase and pancreatic lipase inhibitory activities as well as their anti-proliferative potential were screened. The aroma profile of the leaves, flowers, and flowers at pre-flowering stages of S. judaica, obtained through SPME was composed of sesquiterpene hydrocarbons (87.7 %, 71.8 %, and 86.2 %, respectively) while the hydro-distilled oil of the dry leaves was rich in oxygenated sesquiterpenes (50.8%). Fresh leaves of S. multicaulis were rich in oxygenated monoterpenes (58.1%), while monoterpene hydrocarbons dominated the blooming flowers (57.2%) and the flowers at the pre-flowering stage (64.7%). The hydro-distilled oil of the dry leaves was rich in oxygenated monoterpenes (77.6%). With doxorubicin as a positive control, no anti-proliferative activity was observed against colorectal cancer cell lines HT29, HCT116, and SW620 using SRB assay for either Salvia spp. In vitro enzymatic starch digestion was evaluated with Acarbose (IC50: 0.2 ± 0.0 µg /mL) as the reference drug. The respective IC50 (mg/mL) values of S. judaica and S. multicaulis aqueous extracts were 4.9 ± 0.4 and 10.3 ± 0.9. Modulation of pancreatic lipase activity (PL) was determined by colorimetry and compared with Orlistat (IC50 : 0.11 ± 0.0 µg/mL). PL-IC50 values (µg/mL) obtained for S. judaica and S. multicaulis were 108.5±6.4 and 31.8 ± 0.8, respectively.
authors.forename: Fatma U
authors.initials: FU
authors.lastname: Afifi
authors.forename: Violet
authors.initials: V
authors.lastname: Kasabri
authors.forename: Hala I
authors.initials: HI
authors.lastname: Al-Jaber
authors.forename: Barakat E
authors.initials: BE
authors.lastname: Abu-Irmaileh
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Al-Qudah
authors.forename: Ismail F
authors.initials: IF
authors.lastname: Abazaa
mesh_terms: D004354:Drug Screening Assays, Antitumor; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D007597:Jordan; D008049:Lipase / Q000037:antagonists & inhibitors; D009822:Oils, Volatile / Q000737:chemistry*; D027544:Salvia / Q000737:chemistry*
chemical_list: D009822:Oils, Volatile; D008049:Lipase