title: Cationic lioposomes with folic acid as targeting ligand for gene delivery.
abstract: In our previous Letter, we have carried out the synthesis of a novel DDCTMA cationic lipid which was formulated with DOPE for gene delivery. Herein, we used folic acid (FA) as targeting ligand and cholesterol (Chol) as helper lipid instead of DOPE for enhancing the stability of the liposomes. These liposomes were characterized by dynamic laser scattering (DLS), transmission electron microscopy (TEM) and agarose gel electrophoresis assays of pDNA binding affinity. The lipoplexes were prepared by using different weight ratios of DDCTMA/Chol (1:1, 2:1, 3:1, 4:1) liposomes and different concentrations of FA (50-200μg/mL) combining with pDNA. The transfection efficiencies of the lipoplexes were evaluated using pGFP-N2 and pGL3 plasmid DNA against NCI-H460 cells in vitro. Among them, the optimum gene transfection efficiency with DDCTMA/Chol (3:1)/FA (100μg/mL) was obtained. The results showed that FA could improve the gene transfection efficiencies of DDCTMA/Chol cationic liposome. Our results also convincingly demonstrated FA (100μg/mL)-coated DDCTMA/Chol (3:1) cationic liposome could serve as a promising candidate for the gene delivery.
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bio-resources Utilization, College of Life Science, Dalian Nationalities University, Dalian, Liaoning 116600, China.
authors.forename: Shao-Hui
authors.initials: SH
authors.lastname: Cui
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bio-resources Utilization, College of Life Science, Dalian Nationalities University, Dalian, Liaoning 116600, China.
authors.forename: De-Fu
authors.initials: DF
authors.lastname: Zhi
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bio-resources Utilization, College of Life Science, Dalian Nationalities University, Dalian, Liaoning 116600, China.
authors.forename: Yi-Nan
authors.initials: YN
authors.lastname: Zhao
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bio-resources Utilization, College of Life Science, Dalian Nationalities University, Dalian, Liaoning 116600, China.
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Chen
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bio-resources Utilization, College of Life Science, Dalian Nationalities University, Dalian, Liaoning 116600, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Meng
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bio-resources Utilization, College of Life Science, Dalian Nationalities University, Dalian, Liaoning 116600, China.
authors.forename: Chuan-Min
authors.initials: CM
authors.lastname: Zhang
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bio-resources Utilization, College of Life Science, Dalian Nationalities University, Dalian, Liaoning 116600, China. Electronic address: zsb@dlnu.edu.cn.
authors.forename: Shu-Biao
authors.initials: SB
authors.lastname: Zhang
mesh_terms: D002412:Cations / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000737:chemistry; D000067493:Dynamic Light Scattering; D005492:Folic Acid / Q000737:chemistry*; D017930:Genes, Reporter; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D014162:Transfection
chemical_list: D002412:Cations; D008081:Liposomes; D005492:Folic Acid; D002784:Cholesterol
keywords: Cationic liposome; Folate receptor; Folic acid; Gene delivery; Transfection efficiency