title: Qingchang Wenzhong Decoction Ameliorates Dextran Sulphate Sodium-Induced Ulcerative Colitis in Rats by Downregulating the IP10/CXCR3 Axis-Mediated Inflammatory Response.
abstract: Qingchang Wenzhong Decoction (QCWZD) is an effective traditional Chinese medicine prescription. Our previous studies have shown that QCWZD has significant efficacy in patients with mild-to-moderate ulcerative colitis (UC) and in colonic mucosa repair in UC rat models. However, the exact underlying mechanism remains unknown. Thus, this study was conducted to determine QCWZD's efficacy and mechanism in dextran sulphate sodium- (DSS-) induced UC rat models, which were established by 7-day administration of 4.5% DSS solution. QCWZD was administered daily for 7 days, after which the rats were euthanized. Disease activity index (DAI), histological score (HS), and myeloperoxidase (MPO) level were determined to evaluate UC severity. Serum interferon gamma-induced protein 10 (IP10) levels were determined using ELISA kits. Western blotting and real-time polymerase chain reaction were, respectively, used to determine colonic protein and gene expression of IP10, chemokine (cys-x-cys motif) receptor (CXCR)3, and nuclear factor- (NF-) κB p65. Intragastric QCWZD administration ameliorated DSS-induced UC, as evidenced by decreased DAI, HS, and MPO levels. Furthermore, QCWZD decreased the protein and gene expression of IP10, CXCR3, and NF-κB p65. Overall, these results suggest that QCWZD ameliorates DSS-induced UC in rats by downregulating the IP10/CXCR3 axis-mediated inflammatory response and may be a novel UC therapy.
authors.affiliation: Beijing University of Chinese Medicine, No. 11, North Third Ring East Road, Beijing 100029, China; Gastroenterology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6, 1st Section, Fangxingyuan, Fangzhuang, Beijing 100078, China.
authors.forename: Tang-You
authors.initials: TY
authors.lastname: Mao
authors.affiliation: Gastroenterology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6, 1st Section, Fangxingyuan, Fangzhuang, Beijing 100078, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.affiliation: Beijing University of Chinese Medicine, No. 11, North Third Ring East Road, Beijing 100029, China; Gastroenterology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6, 1st Section, Fangxingyuan, Fangzhuang, Beijing 100078, China.
authors.forename: Wei-Han
authors.initials: WH
authors.lastname: Zhao
authors.affiliation: Beijing University of Chinese Medicine, No. 11, North Third Ring East Road, Beijing 100029, China; Gastroenterology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6, 1st Section, Fangxingyuan, Fangzhuang, Beijing 100078, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Beijing University of Chinese Medicine, No. 11, North Third Ring East Road, Beijing 100029, China; Gastroenterology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6, 1st Section, Fangxingyuan, Fangzhuang, Beijing 100078, China.
authors.forename: Kang-Li
authors.initials: KL
authors.lastname: Gao
authors.affiliation: Beijing University of Chinese Medicine, No. 11, North Third Ring East Road, Beijing 100029, China; Gastroenterology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6, 1st Section, Fangxingyuan, Fangzhuang, Beijing 100078, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Beijing University of Chinese Medicine, No. 11, North Third Ring East Road, Beijing 100029, China; Gastroenterology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6, 1st Section, Fangxingyuan, Fangzhuang, Beijing 100078, China.
authors.forename: Tian-Hong
authors.initials: TH
authors.lastname: Xie
authors.affiliation: Gastroenterology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6, 1st Section, Fangxingyuan, Fangzhuang, Beijing 100078, China.
authors.forename: Jun-Xiang
authors.initials: JX
authors.lastname: Li