title: Novel polymorphism in exon 1 of the melatonin receptor gene unassociated with reproductive characteristics of buffaloes in the Amazon Region.
abstract: The objective of this study was to sequence part of the exon 1 in the melatonin receptor 1A gene (MTRN1A) in buffaloes to detect a novel polymorphism with which to associate reproductive characteristics, such as age at first birth and the interval between births, in buffaloes from the northeastern region of the State of Pará (Brazil). Buffalo hair samples (77) were collected from the Terra Firme region of Pará. DNA was extracted and polymerase chain reactions (PCRs) were carried out with a primer that was designed using the GenBank accession No. AY524665 reference sequence. PCR products were purified and sequenced. After editing and analysis of the sequences, a mutation was observed at the 62nd position in exon 1 of MTRN1A (T↔C), which corresponded with a change in the 21st amino acid from leucine to proline. All possible genotypes were observed, with the most common being genotype CC (0.481). The allele frequencies were T = 0.377 and C = 0.623. Statistical analysis of FIS showed inbreeding within the sample group (FIS = 0.397) and deviations from the Hardy- Weinberg equilibrium were observed (P < 0.05). Associations between genotypes and reproductive characteristics were not significant (P > 0.05). Although the related SNP was not synonymous, there were no observable effects on the reproductive characteristics under investigation. As such, it would be ideal to detect other SNPs in exon 1 of the MTRN1A gene that can be associated with reproductive characteristics in Amazonian buffaloes.
authors.affiliation: Programa de Pós-Graduação em Ciência Animal, Núcleo de Ciência Animal, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: E M
authors.initials: EM
authors.lastname: Barbosa
authors.affiliation: Laboratório de Reprodução Animal, Instituto de Saúde e Produção Animal, Universidade Federal Rural da Amazônia, Belém, PA, Brasil.
authors.forename: B B
authors.initials: BB
authors.lastname: Souza
authors.affiliation: Laboratório de Reprodução Animal, Instituto de Saúde e Produção Animal, Universidade Federal Rural da Amazônia, Belém, PA, Brasil.
authors.forename: R C
authors.initials: RC
authors.lastname: Guimarães
authors.affiliation: Laboratório de Biologia Geral, Universidade Federal Rural da Amazônia, Campus de Capanema, Capanema, PA, Brasil.
authors.forename: J S N
authors.initials: JS
authors.lastname: Azevedo
authors.affiliation: Laboratório de Tecnologia Biomolecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: E C
authors.initials: EC
authors.lastname: Gonçalves
authors.affiliation: Laboratório de Reprodução Animal, Instituto de Saúde e Produção Animal, Universidade Federal Rural da Amazônia, Belém, PA, Brasil.
authors.forename: H F L
authors.initials: HF
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Reprodução Animal, Instituto de Saúde e Produção Animal, Universidade Federal Rural da Amazônia, Belém, PA, Brasil.
authors.forename: S T
authors.initials: ST
authors.lastname: Rolim Filho
authors.affiliation: Laboratório de Genética Animal, Instituto de Ciências Agrárias, Universidade Federal Rural da Amazônia, Belém, PA, Brasil.
authors.forename: E
authors.initials: E
authors.lastname: Silva Filho
mesh_terms: D000818:Animals; D001938:Brazil; D001947:Breeding; D002020:Buffaloes / Q000235:genetics* / Q000378:metabolism; D005091:Exons; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D008550:Melatonin / Q000378:metabolism; D010298:Parity; D020641:Polymorphism, Single Nucleotide; D044094:Receptors, Melatonin / Q000235:genetics* / Q000378:metabolism; D012098:Reproduction / Q000235:genetics
chemical_list: D044094:Receptors, Melatonin; D008550:Melatonin