title: Oxidative and nitrosative stress in serum of patients with Parkinson's disease.
abstract: Parkinson's disease (PD) is one of the common neurodegenerative disorders. Oxidative stress is considered as a contributing factor to the development of PD. The present study aims to investigate serum oxidative stress status in patients with PD. Oxidative stress was assessed by measuring serum nitric oxide levels, lipid hydroperoxide concentrations, and nitric oxide synthase activity. In addition, total serum antioxidant capacity (TAC) was evaluated using the serum 2,2-Diphenyl-1-picryl-hydrazyl (DPPH) free-radical scavenging method in 32 patient with Parkinson's disease and 32 control subjects. Our results indicated that serum nitric oxide and lipid hydroperoxide levels were significantly lower in patients with PD than controls. Moreover, nitric oxide levels were found to be negatively correlated with Unified Parkinson's Disease Rating Scale (UPDRS). However, no statistical difference was observed in total serum antioxidant capacities and nitric oxide synthase activities between patients and controls. The present study indicates that although antioxidant capacity was not changed, lipid hydroperoxide (LPO) level was found decreased. This might show pre-oxidative process in these patients. In addition, decreased nitric oxide (NO) level and negative correlation observed between NO level and disease rating scale implicated a role for NO in the disease process.
authors.affiliation: Department of Medical Biochemistry, Ankara University Faculty of Medicine, Morphology Building, Sıhhiye, 06100, Ankara, Turkey. hikmetcancubukcu@gmail.com.
authors.forename: Hikmet Can
authors.initials: HC
authors.lastname: Çubukçu
authors.affiliation: Department of Neurology, Ankara Training and Research Hospital, Ankara, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Yurtdaş
authors.affiliation: The Laboratory of Turkish Public Health Institution, Ankara, Turkey.
authors.forename: Zahide Esra
authors.initials: ZE
authors.lastname: Durak
authors.affiliation: Directorate of Health Services, Turkish Ministry of Health, Ankara, Turkey.
authors.forename: Bilal
authors.initials: B
authors.lastname: Aytaç
authors.affiliation: Department of Neurology, Ankara Training and Research Hospital, Ankara, Turkey.
authors.forename: Hafize Nalan
authors.initials: HN
authors.lastname: Güneş
authors.affiliation: Department of Neurology, Ankara Training and Research Hospital, Ankara, Turkey.
authors.forename: Burcu Gökçe
authors.initials: BG
authors.lastname: Çokal
authors.affiliation: Department of Neurology, Ankara Training and Research Hospital, Ankara, Turkey.
authors.forename: Tahir Kurtuluş
authors.initials: TK
authors.lastname: Yoldaş
authors.affiliation: Department of Medical Biochemistry, Ankara University Faculty of Medicine, Morphology Building, Sıhhiye, 06100, Ankara, Turkey.
authors.forename: İlker
authors.initials: İ
authors.lastname: Durak
mesh_terms: D000368:Aged; D005260:Female; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood*; D018384:Oxidative Stress / Q000502:physiology*; D010300:Parkinson Disease / Q000097:blood*; D014421:Turkey
chemical_list: D008054:Lipid Peroxides; D009569:Nitric Oxide
keywords: Nitric oxide; Oxidative stress; Parkinson’s disease