title: Integrative volumetric bar-chart chip for rapid and quantitative point-of-care detection of myocardial infarction biomarkers.
abstract: Here we developed an integrated volumetric bar-chart chip (IV-Chip) technology by integration of our previous V-Chip with a fluid handling design to generate an instrument-free POC device and greatly reduce the detection time and effort. The IV-Chip test requires only 1 μL of serum separated from finger-prick blood. The serum sample and ELISA reagents are directly loaded into the device using a pipette, and a shift of the two layers of the device generates homogeneous liquid segments in the microfluidic channel. Under vacuum pressure generated by a regular syringe, the segments flow into the ELISA wells in sequence and a sandwich ELISA reaction takes place. As a result of the automated washing and reacting strategy, the IV-Chip allows rapid tests for myocardial infarction biomarkers, and turnaround time is greatly reduced to 15 min. The specificity and accuracy of quantitative multiplex detection of MI biomarkers CK-MB, troponin I and myoglobin, are 87.5% and 95.8%, respectively.
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Ave, Houston, TX 77030, USA. Lqin@houstonmethodist.org.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Song
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Wang
authors.forename: Wenjin
authors.initials: W
authors.lastname: Qi
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Xuan
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Lidong
authors.initials: L
authors.lastname: Qin
mesh_terms: D055910:Antibodies, Immobilized / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000378:metabolism; D057205:Automation, Laboratory; D015415:Biomarkers / Q000097:blood; D017076:Computer-Aided Design; D052279:Creatine Kinase, MB Form / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D004867:Equipment Design; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis; D009211:Myoglobin / Q000097:blood*; D010984:Platinum / Q000737:chemistry; D000067716:Point-of-Care Testing; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties; D019210:Troponin I / Q000097:blood*
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D009211:Myoglobin; C581522:TNNI1 protein, human; D019210:Troponin I; D010984:Platinum; D052279:Creatine Kinase, MB Form