title: Downregulation of miR-154 in human glioma and its clinicopathological and prognostic significance.
abstract: Objective MicroRNA-154 (miR-154) was previously reported to be downregulated in several types of human cancers and may act as a tumour suppressor. This study aimed to measure miR-154 levels and determine its clinical significance in human glioma. Methods This retrospective study analysed fresh human glioma specimens and non-neoplastic brain tissues using real-time quantitative reverse transcription-polymerase chain reaction to determine the relative levels of miR-154. The association between miR-154 levels and various clinicopathological characteristics and survival was analysed. Results A total of 115 patients with gliomas and 115 non-neoplastic brain tissues were examined. MiR-154 levels were significantly downregulated in gliomas compared with non-neoplastic brain tissues. Low levels of miR-154 were associated with high World Health Organization grade, large tumour size (≥ 5 cm), a low Karnofsky performance status score (< 80), and a shorter overall survival. Multivariate analyses using the Cox proportional hazards regression model confirmed that decreased miR-154 level was an independent predictor of a poor prognosis. Conclusions These results suggest that miR-154 downregulation may be involved in glioma formation and progression, and that miR-154 might serve as a potential prognostic biomarker for patients with this disease.
authors.affiliation: 1 Department of Neurology, First Hospital of Jilin University, Changchun, Jilin Province, China.|2 Department of Neurology, Affiliated Hospital of Beihua University, Jilin City, Jilin Province, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Wang
authors.affiliation: 3 Department of Thoracic Surgery, Jilin Central Hospital, Jilin City, Jilin Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.affiliation: 1 Department of Neurology, First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D017567:Karnofsky Performance Status; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies
chemical_list: D014408:Biomarkers, Tumor; C561122:MIRN154 microRNA, human; D035683:MicroRNAs
keywords: MicroRNA-154; glioma; prognosis; real-time quantitative reverse transcription–polymerase chain reaction