title: Detergent assisted lipid extraction from wet yeast biomass for biodiesel: A response surface methodology approach.
abstract: The lipid extraction from the microbial biomass is a tedious and high cost dependent process. In the present study, detergent assisted lipids extraction from the culture of the yeast Yarrowia lipolytica SKY-7 was carried out. Response surface methodology (RSM) was used to investigate the effect of three principle parameters (N-LS concentration, time and temperature) on microbial lipid extraction efficiency % (w/w). The results obtained by statistical analysis showed that the quadratic model fits in all cases. Maximum lipid recovery of 95.3±0.3% w/w was obtained at the optimum level of process variables [N-LS concentration 24.42mg (equal to 48mgN-LS/g dry biomass), treatment time 8.8min and reaction temperature 30.2°C]. Whereas the conventional chloroform and methanol extraction to achieve total lipid recovery required 12h at 60°C. The study confirmed that oleaginous yeast biomass treatment with N-lauroyl sarcosine would be a promising approach for industrial scale microbial lipid recovery.
authors.affiliation: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors.forename: Sravan Kumar
authors.initials: SK
authors.lastname: Yellapu
authors.affiliation: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors.forename: Jyothi
authors.initials: J
authors.lastname: Bezawada
authors.affiliation: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors.forename: Rajwinder
authors.initials: R
authors.lastname: Kaur
authors.affiliation: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors.forename: Mathiazhakan
authors.initials: M
authors.lastname: Kuttiraja
authors.affiliation: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada. Electronic address: rd.tyagi@ete.inrs.ca.
authors.forename: Rajeshwar D
authors.initials: RD
authors.lastname: Tyagi
mesh_terms: D056804:Biofuels; D018533:Biomass; D003902:Detergents / Q000737:chemistry*; D008055:Lipids / Q000302:isolation & purification*; D000432:Methanol; D015233:Models, Statistical; D012521:Sarcosine / Q000031:analogs & derivatives; D013696:Temperature; D025062:Yarrowia / Q000737:chemistry*
chemical_list: D056804:Biofuels; D003902:Detergents; D008055:Lipids; C025231:sarkosyl; D000432:Methanol; D012521:Sarcosine
keywords: Biodiesel; Box-Behnken design; Lipid extraction; N-lauroyl sarcosine