title: Altered expression of neuronal CCR6 during pilocarpine induced status epilepticus in mice.
abstract: Chemokine and receptor systems play important roles in different animal models of status epileptics and epileptogenesis. Here, we identified protein and gene expression of chemokine receptor 6 in the hippocampus of Swiss mice with immunohistochemistry and RT-PCR respectively. Immunohistochemical study showed that CCR6 immunopositive product was localized in different subtypes of hippocampal interneurons, in apical dendrites of pyramidal neurons of CA1 area and other laminas of the hippocampus. Strongly stained CCR6 immunopositive product was found in calbindin, calretinin, parvalbumin immunopositive interneurons in the stratum oriens of CA1 area. During pilocarpine induced status epilepticus, a transient down-regulation of neuronal CCR6 in the stratum oriens of CA1 was demonstrated at 2h during status epilepticus. The present study provides evidence that CCR6 may be involved in normal neuronal activity in the hippocampus and play an important role in maintenance of the status epilepticus.
authors.affiliation: Institute of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an 710061, China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Liu
authors.affiliation: The Second Affiliated Hospital of Kunming Medical University, 374 Dianmian Road, Kunming 650101, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Cao
authors.affiliation: Institute of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an 710061, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Radiobiology Research Laboratory, Singapore Nuclear Research and Safety Initiative, National University of Singapore, Singapore. Electronic address: tangfr@gmail.com.
authors.forename: Feng-Ru
authors.initials: FR
authors.lastname: Tang
mesh_terms: D000818:Animals; D064032:Calbindin 2 / Q000378:metabolism; D064026:Calbindins / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D059330:GABAergic Neurons / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D007395:Interneurons / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010320:Parvalbumins / Q000378:metabolism; D010862:Pilocarpine; D016133:Polymerase Chain Reaction; D017966:Pyramidal Cells / Q000378:metabolism* / Q000473:pathology; D054399:Receptors, CCR6 / Q000378:metabolism*; D013226:Status Epilepticus / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
chemical_list: C498126:CCR6 protein, mouse; C576511:Calb2 protein, mouse; D064032:Calbindin 2; D064026:Calbindins; D010320:Parvalbumins; D054399:Receptors, CCR6; D010862:Pilocarpine
keywords: Chemokine receptor; Epilepsy; Hippocampus; Status epilepticus