title: Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates.
abstract: A series of fluorogenic enzymatic substrates that incorporate a self-immolative spacer were synthesised for the purpose of identifying l-alanylaminopeptidase activity in microorganisms in agar media. These substrates resulted in the generation of fluorescent microorganism colonies with Gram-negative microorganisms.
authors.affiliation: Research & Development Microbiology, bioMérieux SA, 3 route de Port Michaud, 38 390 La-Balme-les-Grottes, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Cellier
authors.affiliation: Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Arthur L
authors.initials: AL
authors.lastname: James
authors.affiliation: Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Lowe
authors.affiliation: Research & Development Microbiology, bioMérieux SA, 3 route de Port Michaud, 38 390 La-Balme-les-Grottes, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Orenga
authors.affiliation: Department of Microbiology, Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK.
authors.forename: John D
authors.initials: JD
authors.lastname: Perry
authors.affiliation: Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Ari K
authors.initials: AK
authors.lastname: Rasul
authors.affiliation: Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Stanforth
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D018826:CD13 Antigens / Q000032:analysis / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D013379:Substrate Specificity; D015003:Yeasts / Q000201:enzymology*
chemical_list: D005456:Fluorescent Dyes; D018826:CD13 Antigens
keywords: Fluorogenic enzyme substrates; Microorganisms; l-Alanylaminopeptidase detection