title: Lab-on-a-disc for simultaneous determination of total phenolic content and antioxidant activity of beverage samples.
abstract: In this paper, we present a fully integrated and automated lab-on-a-disc for the rapid determination of the total phenolic content (TPC) and antioxidant activity (AA) of beverage samples. The simultaneous determinations of TPC and AA on a spinning disc were achieved by integrating three independent analytical techniques: the Folin-Ciocalteu method that is used to measure TPC, the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) method and the ferric reducing antioxidant power method that are used to measure AA. The TPC and AA of 8 different beverage samples, including various fruit juices, tea, wine and beer, were analyzed. Unlike conventional labor-intensive processes for measuring TPC and AA, our fully automated platform offers one-step operation and rapid analysis.
authors.affiliation: Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok 10400, Thailand. rattikan.cha@mahidol.ac.th.
authors.forename: Apichai
authors.initials: A
authors.lastname: Phonchai
authors.affiliation: Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok 10400, Thailand. rattikan.cha@mahidol.ac.th.
authors.forename: Rattikan
authors.initials: R
authors.lastname: Chantiwas
authors.affiliation: Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea and Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea. ykcho@unist.ac.kr.
authors.forename: Yoon-Kyoung
authors.initials: YK
authors.lastname: Cho
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D057205:Automation, Laboratory / Q000295:instrumentation; D001628:Beverages / Q000032:analysis*; D001713:Biphenyl Compounds / Q000737:chemistry; D004867:Equipment Design; D005290:Ferric Compounds / Q000737:chemistry; D005504:Food Analysis / Q000295:instrumentation*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D008982:Molybdenum / Q000737:chemistry; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis*; D010851:Picrates / Q000737:chemistry; D015203:Reproducibility of Results; D017973:Tungsten Compounds / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005290:Ferric Compounds; C029556:Folin's phenol reagent; D007202:Indicators and Reagents; D010636:Phenols; D010851:Picrates; D017973:Tungsten Compounds; D008982:Molybdenum; C004931:1,1-diphenyl-2-picrylhydrazyl