title: Synthesis and evaluation of a maltose-bonded silica gel stationary phase for hydrophilic interaction chromatography and its application in Ginkgo Biloba extract separation in two-dimensional systems.
abstract: Maltose covalently bonded to silica was prepared by using carbonyl diimidazole as a cross-linker and employed as a stationary phase for hydrophilic interaction liquid chromatography. The column efficiency and the effect of water content, buffer concentration, and pH value influenced on retention were investigated. The separation or enrichment selectivity was also studied with nucleosides, saccharides, amino acids, peptides, and glycopeptides. The results indicated that the stationary phase processed good separation efficiency and separation selectivity in hydrophilic interaction liquid chromatography mode. Moreover, a two-dimensional hydrophilic interaction liquid chromatography× reversed-phase liquid chromatography method with high orthogonality was developed to analyze the Ginkgo Biloba extract fractions. The development of this two-dimensional chromatographic system would be an effective tool for the separation of complex samples of different polarities and contents.
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, China.
authors.forename: Qianying
authors.initials: Q
authors.lastname: Sheng
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, China.
authors.forename: Kaiya
authors.initials: K
authors.lastname: Yang
authors.affiliation: Engineering Research Center of Pharmaceutical Process Chemistry, East China University of Science and Technology, Shanghai, China.
authors.forename: Yanxiong
authors.initials: Y
authors.lastname: Ke
authors.affiliation: Engineering Research Center of Pharmaceutical Process Chemistry, East China University of Science and Technology, Shanghai, China.
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, China. minbolan@ecust.edu.cn.
authors.forename: Minbo
authors.initials: M
authors.lastname: Lan
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D020441:Ginkgo biloba / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008320:Maltose / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D058428:Silica Gel / Q000737:chemistry; D000096983:Ginkgo Extract
chemical_list: D008320:Maltose; D010936:Plant Extracts; D058428:Silica Gel; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract
keywords: Ginkgo Biloba extract; Hydrophilic interaction liquid chromatography; Saccharide-modified stationary phases; Separation selectivity; Two-dimensional chromatography