title: Vitamin A Deficiency Decreases the Expression of RARβ and RXRβ/γ in Adult Mouse Brain: Effect of RA Administration.
abstract: Recent studies have revealed a novel and unexpected role of vitamin A, via its nuclear receptors, in higher cognitive functions. We examined, in mouse brain, the impact of vitamin A status on the level of retinoic acid nuclear receptor (RAR and RXR) expression and on one of their responsive genes, that of the tissue transglutaminase (tTG). Weanling male C57B1/6 mice fed a vitamin A deficient diet developed a vitamin A deficiency which was characterized, after 26 weeks, by the depletion of serum retinol, liver retinol and retinyl palmitate, and by the decreased activity of liver tTG. After 27 weeks of the diet, the vitamin A depleted mice exhibited a significantly lower amount of brain RAR β and RXR β/γ mRNA relative to control mice. Vitamin A deficiency also resulted in a reduced expression of tTG. When 35-week depleted mice were subsequently administrated RA for 28 days, the expression of RA nuclear receptors and tTG was significantly induced. The vitamin A deficiency mouse model and repletion provides a physiological system for monitoring the effects of vitamin A status on gene expression and on neurobiological processes in the adult brain.
authors.affiliation: a Laboratoire de Nutrition et Signalisation Cellulaire , ISTAB , Avenue des Facultés , Université Bordeaux I , 33405 Talence, France.
authors.forename: V
authors.initials: V
authors.lastname: Enderlin
authors.affiliation: b Laboratoire de Biochimie , Hopital Pellegrin , 33076 Bordeaux , France.
authors.forename: D
authors.initials: D
authors.lastname: Higueret
authors.affiliation: a Laboratoire de Nutrition et Signalisation Cellulaire , ISTAB , Avenue des Facultés , Université Bordeaux I , 33405 Talence, France.
authors.forename: S
authors.initials: S
authors.lastname: Alfos
authors.affiliation: a Laboratoire de Nutrition et Signalisation Cellulaire , ISTAB , Avenue des Facultés , Université Bordeaux I , 33405 Talence, France.
authors.forename: M
authors.initials: M
authors.lastname: Husson
authors.affiliation: c Laboratoire de Neurosciences Cognitives (UMR CNRS 5807) , Université de Bordeaux I , 33405 Talence, France.
authors.forename: R
authors.initials: R
authors.lastname: Jaffard
authors.affiliation: a Laboratoire de Nutrition et Signalisation Cellulaire , ISTAB , Avenue des Facultés , Université Bordeaux I , 33405 Talence, France.
authors.forename: P
authors.initials: P
authors.lastname: Higueret
authors.affiliation: a Laboratoire de Nutrition et Signalisation Cellulaire , ISTAB , Avenue des Facultés , Université Bordeaux I , 33405 Talence, France.
authors.forename: V
authors.initials: V
authors.lastname: Pallet
keywords: (RAR, RXR); Adult brain; Retinoic acid receptors; Tissue transglutaminase; Vitamin A deficiency