title: Rabies virus inactivates cofilin to facilitate viral budding and release.
abstract: Cytoplasmic actin and actin-associated proteins have been identified in RABV particles. Although actin is involved in RABV entry into cells, the specific role of actin in RABV budding and release remains unknown. Our study found that RABV M protein-mediated virion budding depends on intact actin filaments. Confocal microscopy demonstrated a block to virions budding, with a number of M protein-mediated budding vesicles detained in the cell cytoplasm. Furthermore, RABV infection resulted in inactivation of cofilin and upregulation of phosphorylated cofilin. Knockdown of cofilin reduced RABV release. These results for the first time indicate that RABV infection resulted in upregulation of phosphorylated cofilin to facililtate actin polymerization for virus budding.
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zan
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, PR China.
authors.forename: Shu-Ting
authors.initials: ST
authors.lastname: An
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, PR China.
authors.forename: Kai-Kun
authors.initials: KK
authors.lastname: Mo
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, PR China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, PR China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, PR China. Electronic address: liaomin4545@zju.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Liao
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, PR China; Institute of Immunology, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Ji-Yong
authors.initials: JY
authors.lastname: Zhou
mesh_terms: D051339:Actin Depolymerizing Factors / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D015536:Down-Regulation / Q000502:physiology; D051379:Mice; D009474:Neurons / Q000821:virology*; D011820:Rabies virus / Q000502:physiology*; D014763:Viral Matrix Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D057074:Virus Release / Q000502:physiology*
chemical_list: D051339:Actin Depolymerizing Factors; D014763:Viral Matrix Proteins; D014764:Viral Proteins
keywords: Actin filaments; Cofilin; Cytochalasin D; Rabies virus; Viral budding