title: Protective effect of vitamin E on sperm motility and oxidative stress in valproic acid treated rats.
abstract: Long-term administration of valproic acid (VPA) is known to promote reproductive impairment mediated by increase in testicular oxidative stress. Vitamin E (VitE) is a lipophilic antioxidant known to be essential for mammalian spermatogenesis. However, the capacity of this vitamin to abrogate the VPA-mediated oxidative stress has not yet been assessed. In the current study, we evaluated the protective effect of VitE on functional abnormalities related to VPA-induced oxidative stress in the male reproductive system. VPA (400 mg kg(-1)) was administered by gavage and VitE (50 mg kg(-1)) intraperitoneally to male Wistar rats for 28 days. Analysis of spermatozoa from the cauda epididymides was performed. The testes and epididymides were collected for measurement of oxidative stress biomarkers. Treatment with VPA induced a decrease in sperm motility accompanied by an increase in oxidative damage to lipids and proteins, depletion of reduced glutathione and a decrease in total reactive antioxidant potential on testes and epididymides. Co-administration of VitE restored the antioxidant potential and prevented oxidative damage on testes and epididymides, restoring sperm motility. Thus, VitE protects the reproductive system from the VPA-induced damage, suggesting that it may be a useful compound to minimize the reproductive impairment in patients requiring long-term treatment with VPA.
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.
authors.forename: Giovana M
authors.initials: GM
authors.lastname: Ourique
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.
authors.forename: Etiane M H
authors.initials: EM
authors.lastname: Saccol
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.
authors.forename: Tanise S
authors.initials: TS
authors.lastname: Pês
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.
authors.forename: Werner G
authors.initials: WG
authors.lastname: Glanzner
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Schiefelbein
authors.affiliation: Department of Morphology, Federal University of Santa Catarina (UFSC), Florianópolis, SC 88040-900, Brazil.
authors.forename: Viviane M
authors.initials: VM
authors.lastname: Woehl
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.
authors.forename: Bernardo
authors.initials: B
authors.lastname: Baldisserotto
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Pavanato
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.
authors.forename: Paulo B D
authors.initials: PB
authors.lastname: Gonçalves
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil. Electronic address: barreto.kp@gmail.com.
authors.forename: Kátia P
authors.initials: KP
authors.lastname: Barreto
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology*; D001681:Biological Assay; D004822:Epididymis / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects*; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000187:drug effects; D013739:Testosterone / Q000032:analysis; D014635:Valproic Acid / Q000633:toxicity*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000927:Anticonvulsants; D000975:Antioxidants; D020011:Protective Agents; D014810:Vitamin E; D013739:Testosterone; D014635:Valproic Acid; D005978:Glutathione
keywords: Alpha-tocopherol; Antioxidants; Oxidative damage; Reproductive toxicity; Sodium valproate