title: Data for amino acid alignment of Japanese stingray melanocortin receptors with other gnathostome melanocortin receptor sequences, and the ligand selectivity of Japanese stingray melanocortin receptors.
abstract: This article contains structure and pharmacological characteristics of melanocortin receptors (MCRs) related to research published in "Characterization of melanocortin receptors from stingray Dasyatis akajei, a cartilaginous fish" (Takahashi et al., 2016) [1]. The amino acid sequences of the stingray, D. akajei, MC1R, MC2R, MC3R, MC4R, and MC5R were aligned with the corresponding melanocortin receptor sequences from the elephant shark, Callorhinchus milii, the dogfish, Squalus acanthias, the goldfish, Carassius auratus, and the mouse, Mus musculus. These alignments provide the basis for phylogenetic analysis of these gnathostome melanocortin receptor sequences. In addition, the Japanese stingray melanocortin receptors were separately expressed in Chinese Hamster Ovary cells, and stimulated with stingray ACTH, α-MSH, β-MSH, γ-MSH, δ-MSH, and β-endorphin. The dose response curves reveal the order of ligand selectivity for each stingray MCR.
authors.affiliation: School of Marine Biosciences, Kitasato Universtiy, Sagaminara 252-0373, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Takahashi
authors.affiliation: Department of Biological Sciences, University of Denver, Denver, CO 80210, USA.
authors.forename: Perry
authors.initials: P
authors.lastname: Davis
authors.affiliation: Department of Biological Sciences, University of Denver, Denver, CO 80210, USA.
authors.forename: Christina
authors.initials: C
authors.lastname: Reinick
authors.affiliation: School of Marine Biosciences, Kitasato Universtiy, Sagaminara 252-0373, Japan.
authors.forename: Kanta
authors.initials: K
authors.lastname: Mizusawa
authors.affiliation: Ushimado Marine Institute, Faculty of Sciences, Okayama University, Ushimado, Setouchi 701-4303, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sakamoto
authors.affiliation: Department of Biological Sciences, University of Denver, Denver, CO 80210, USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Dores
keywords: Adrenocorticotropic hormone (ACTH); Dasyatis akajei; Melanocortin receptor (MCR); Melanocyte-stimulating hormone (MSH); Stingray