title: Analyses of Compact Trichinella Kinomes Reveal a MOS-Like Protein Kinase with a Unique N-Terminal Domain.
abstract: Parasitic worms of the genus Trichinella (phylum Nematoda; class Enoplea) represent a complex of at least twelve taxa that infect a range of different host animals, including humans, around the world. They are foodborne, intracellular nematodes, and their life cycles differ substantially from those of other nematodes. The recent characterization of the genomes and transcriptomes of all twelve recognized taxa of Trichinella now allows, for the first time, detailed studies of their molecular biology. In the present study, we defined, curated, and compared the protein kinase complements (kinomes) of Trichinella spiralis and T. pseudospiralis using an integrated bioinformatic workflow employing transcriptomic and genomic data sets. We examined how variation in the kinome might link to unique aspects of Trichinella morphology, biology, and evolution. Furthermore, we utilized in silico structural modeling to discover and characterize a novel, MOS-like kinase with an unusual, previously undescribed N-terminal domain. Taken together, the present findings provide a basis for comparative investigations of nematode kinomes, and might facilitate the identification of Enoplea-specific intervention and diagnostic targets. Importantly, the in silico modeling approach assessed here provides an exciting prospect of being able to identify and classify currently unknown (orphan) kinases, as a foundation for their subsequent structural and functional investigation.
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Andreas J
authors.initials: AJ
authors.lastname: Stroehlein
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Neil D
authors.initials: ND
authors.lastname: Young
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Pasi K
authors.initials: PK
authors.lastname: Korhonen
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia Yourgene Bioscience, Shu-Lin District, New Taipei City 23863, Taiwan.
authors.forename: Bill C H
authors.initials: BC
authors.lastname: Chang
authors.affiliation: Howard Hughes Medical Institute, Chevy Chase, MD 20815-6789 Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91125.
authors.forename: Paul W
authors.initials: PW
authors.lastname: Sternberg
authors.affiliation: Istituto Superiore di Sanità, 00161 Rome, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: La Rosa
authors.affiliation: Istituto Superiore di Sanità, 00161 Rome, Italy.
authors.forename: Edoardo
authors.initials: E
authors.lastname: Pozio
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia robinbg@unimelb.edu.au.
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
mesh_terms: D000818:Animals; D019295:Computational Biology; D016678:Genome; D023281:Genomics; D006801:Humans; D011487:Protein Conformation; D011494:Protein Kinases / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D013045:Species Specificity; D059467:Transcriptome / Q000235:genetics; D014234:Trichinella / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D017160:Trichinella spiralis / Q000235:genetics / Q000472:pathogenicity; D014235:Trichinellosis / Q000201:enzymology / Q000235:genetics* / Q000469:parasitology
chemical_list: D011494:Protein Kinases
keywords: Trichinella; kinome; parasitic worms; protein annotation; protein kinases