title: An aptamer cocktail-functionalized photocatalyst with enhanced antibacterial efficiency towards target bacteria.
abstract: We developed TiO2 particles conjugated with an Escherichia coli surface-specific ssDNA aptamer cocktail (composed of three different aptamers isolated from E. coli) for targeted and enhanced disinfection of E. coli. We examined the target-specific and enhanced inactivation of this composite (TiO2-Apc), which were compared to those of TiO2 conjugated with a single aptamer (one of the three different aptamers, TiO2-Aps) and non-modified TiO2. We found that TiO2-Apc enhanced the inactivation of targeted E. coli under UV irradiation compared to both the non-modified TiO2 and TiO2-Aps. A higher number of TiO2-Apc than TiO2-Aps particles was observed on the surface of E. coli. The amount of TiO2-Apc required to inactivate ∼99.9% of E. coli (10(6) CFU/ml) was 10 times lower than that of non-modified TiO2. The close proximity of functionalized particles with E. coli resulting from the interaction between the target surface and the aptamer induced the efficient and fast transfer of reactive oxygen species to the cells. In a mixed culture of different bacteria (E. coli and Staphylococcus epidermidis), TiO2-Apc enhanced the inactivation of only E. coli. Taken together, these results support the use of aptamer cocktail-conjugated TiO2 for improvement of the target-specific inactivation of bacteria.
authors.affiliation: Center for Environment, Health and Welfare Research, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea.
authors.forename: Min Young
authors.initials: MY
authors.lastname: Song
authors.affiliation: Center for Environment, Health and Welfare Research, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea; Department of Energy and Environmental Engineering, University of Science and Technology (UST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea.
authors.forename: Jongsoo
authors.initials: J
authors.lastname: Jurng
authors.affiliation: School of Environmental Engineering, University of Seoul, Seoulsiripdae-ro 163, Dongdaemun-gu, Seoul 02504, Republic of Korea.
authors.forename: Young-Kwon
authors.initials: YK
authors.lastname: Park
authors.affiliation: Center for Environment, Health and Welfare Research, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea; Department of Energy and Environmental Engineering, University of Science and Technology (UST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea. Electronic address: bchankim@kist.re.kr.
authors.forename: Byoung Chan
authors.initials: BC
authors.lastname: Kim
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002384:Catalysis; D004926:Escherichia coli / Q000737:chemistry / Q000187:drug effects; D053758:Nanoparticles; D010316:Particle Size; D055668:Photochemical Processes; D017382:Reactive Oxygen Species; D013212:Staphylococcus epidermidis / Q000187:drug effects; D014025:Titanium; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D017382:Reactive Oxygen Species; C009495:titanium dioxide; D014025:Titanium
keywords: Antibacterial activity; DNA aptamer cocktail; Photocatalyst; TiO(2)