title: Characterization of neurons in the cortical white matter in human temporal lobe epilepsy.
abstract: The aim of the present work was to characterize neurons in the archi- and neocortical white matter, and to investigate their distribution in mesial temporal sclerosis. Immunohistochemistry and quantification of neurons were performed on surgically resected tissue sections of patients with therapy-resistant temporal lobe epilepsy. Temporal lobe tissues of patients with tumor but without epilepsy and that from autopsy were used as controls. Neurons were identified with immunohistochemistry using antibodies against NeuN, calcium-binding proteins, transcription factor Tbr1 and neurofilaments. We found significantly higher density of neurons in the archi- and neocortical white matter of patients with temporal lobe epilepsy than in that of controls. Based on their morphology and neurochemical content, both excitatory and inhibitory cells were present among these neurons. A subset of neurons in the white matter was Tbr-1-immunoreactive and these neurons coexpressed NeuN and neurofilament marker SMI311R. No colocalization of Tbr1 was observed with the inhibitory neuronal markers, calcium-binding proteins. We suggest that a large population of white matter neurons comprises remnants of the subplate. Furthermore, we propose that a subset of white matter neurons was arrested during migration, highlighting the role of cortical maldevelopment in epilepsy associated with mesial temporal sclerosis.
authors.affiliation: Central Electron Microscopic Laboratory, University of Pécs Medical School, Pécs, Hungary.
authors.forename: Zsófia
authors.initials: Z
authors.lastname: Richter
authors.affiliation: Department of Neurology, University of Pécs Medical School, Pécs, Hungary; MTA-PTE Clinical Neuroscience MR Research Group, University of Pécs, Pécs, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Janszky
authors.affiliation: Department of Medical Biology, University of Pécs Medical School, Pécs, Hungary; Signal Transduction Research Group, János Szentágothai Research Centre, University of Pécs, Pécs, Hungary.
authors.forename: György
authors.initials: G
authors.lastname: Sétáló
authors.affiliation: Department of Neurology, University of Pécs Medical School, Pécs, Hungary; MTA-PTE Clinical Neuroscience MR Research Group, University of Pécs, Pécs, Hungary.
authors.forename: Réka
authors.initials: R
authors.lastname: Horváth
authors.affiliation: Department of Neurosurgery, University of Pécs Medical School, Pécs, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Horváth
authors.affiliation: Department of Neurosurgery, University of Pécs Medical School, Pécs, Hungary; MTA-PTE Clinical Neuroscience MR Research Group, University of Pécs, Pécs, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Dóczi
authors.affiliation: Central Electron Microscopic Laboratory, University of Pécs Medical School, Pécs, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Seress
authors.affiliation: Central Electron Microscopic Laboratory, University of Pécs Medical School, Pécs, Hungary. Electronic address: hajnalka.abraham@aok.pte.hu.
authors.forename: Hajnalka
authors.initials: H
authors.lastname: Ábrahám
mesh_terms: D000328:Adult; D000368:Aged; D034961:Antigens, Nuclear / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000378:metabolism; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D012598:Sclerosis / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D020825:T-Box Domain Proteins / Q000378:metabolism; D013702:Temporal Lobe / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D066127:White Matter / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D055815:Young Adult
chemical_list: D034961:Antigens, Nuclear; D002135:Calcium-Binding Proteins; D009419:Nerve Tissue Proteins; D020825:T-Box Domain Proteins; C574705:TBR1 protein, human; C506808:neuronal nuclear antigen NeuN, human
keywords: epileptic seizures; excitatory neurons; inhibitory neurons; neuronal network; subplate