title: Human embryonic mesenchymal stem cells alleviate pathologic changes of MRL/Lpr mice by regulating Th7 cell differentiation.
abstract: Recent evidence indicates that mesenchymal stem cells (MSC) derived from early embryonic tissues have better therapeutic ability as compared with adult tissue-derived stem cells. In the present study, we transplanted human early embryonic MSC (hMSC) into MRL/Lpr mice via tail vein injection to observe the therapeutic efficacy of hMSC and their impact on T helper 17 (Th17) cell differentiation in MRL/Lpr mice. Animals in hMSC treatment group received hMSC (1 × 106/200 μL) via the tail vein at the age of 16 and 19 weeks. We found that hMSC treatment prolonged the survival of MRL/Lpr mice without inducing tumorigenesis, reduced urine protein, and alleviated the renal pathologic changes. In addition, it reduced the proportion of Th17 cells in the spleen of MRL/Lpr mice and the serum interleukin 17 (IL-17) concentration. Our in vitro experiment also demonstrated that hMSC could secrete Th17 differentiation-related cytokines of PGE2, IL-10 and TGF-β, and IFN-γ stimulation up-regulated the secretion of these immune regulating factors. The results of the present study suggest that hMSC therapy could alleviate systemic and local renal lesions in MRL/Lpr mice, probably by secreting immune regulating factors and regulating Th17 cell differentiation in MRL/Lpr mice.
authors.affiliation: a Division of Nephrology , Affiliated Hospital of Nantong University , Nantong , Jiangsu Province , China.
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.affiliation: a Division of Nephrology , Affiliated Hospital of Nantong University , Nantong , Jiangsu Province , China.
authors.forename: Zhuo-Tao
authors.initials: ZT
authors.lastname: Xiao
authors.affiliation: a Division of Nephrology , Affiliated Hospital of Nantong University , Nantong , Jiangsu Province , China.
authors.forename: Xin-Zhong
authors.initials: XZ
authors.lastname: Huang
authors.affiliation: b Research Center of Developmental Biology and Department of Histology and Embryology , Second Military Medical University , Shanghai , China.
authors.forename: Min-Juan
authors.initials: MJ
authors.lastname: Wu
authors.affiliation: a Division of Nephrology , Affiliated Hospital of Nantong University , Nantong , Jiangsu Province , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.affiliation: c Renal Group, Basic Medical Research Centre, Medical College of Nantong University , Nantong , Jiangsu Province , China.
authors.forename: Ai-Fen
authors.initials: AF
authors.lastname: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D018450:Disease Progression; D053595:Embryonic Stem Cells / Q000166:cytology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D007111:Immunity, Cellular; D020381:Interleukin-17 / Q000378:metabolism; D007668:Kidney / Q000473:pathology*; D008180:Lupus Erythematosus, Systemic / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D019463:Mice, Inbred MRL lpr; D058504:Th17 Cells / Q000276:immunology* / Q000473:pathology
chemical_list: C098680:Il17a protein, mouse; D020381:Interleukin-17
keywords: Embryo; T helper 17 cell; interleukin 17; mesenchymal stem cells; systemic lupus erythematosus