title: The Effect and Mechanisms of Proliferative Inhibition of Crocin on Human Leukaemia Jurkat Cells.
abstract: Targeted therapy is a potentially useful approach for the treatment of T-lineage acute lymphoblastic leukaemia. This study aimed to find a highly effective, low toxic anti-tumour drug and further investigate its mechanisms. Jurkat cells were used as the object and were stimulated by different concentrations of crocin. By cell count, growth curve, MTT method for the detection of cell proliferation, annexin V/propidium iodide (PI) method for the apoptosis rates, and reverse transcription-polymerase chain reaction (RT-PCR) for bcl-2 and bax gene expression, the effect and mechanisms of proliferative inhibition of crocin on Jurkat cells were further explored. Crocin promoted Jurkat cell apoptosis and inhibited cell growth, in a dose-time-dependent manner. The mechanism might be related to the inhibition of bcl-2 gene expression and the promotion of bax gene expression. These results suggest that crocin can be used as a suitable clinical agent for the treatment of T-lineage acute lymphoblastic leukaemia.
authors.affiliation: Department of Paediatric Haematology, the Affiliated Hospital of Qingdao University, Qingdao 266003, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, the Affiliated Hospital of Qingdao University, Qingdao 266003, PR China.
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Paediatrics, Yantai Yu Huang Ding Hospital Affiliated to Qingdao University, Yantai 264000, PR China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Paediatric Haematology, the Affiliated Hospital of Qingdao University, Qingdao 266003, PR China.
authors.forename: L-Z
authors.initials: LZ
authors.lastname: Wang
authors.affiliation: Department of Paediatrics, Wendeng Central Hospital of Weihai, Weihai 264400, PR China.
authors.forename: C
authors.initials: C
authors.lastname: Zang
authors.affiliation: Department of Paediatric Haematology, the Affiliated Hospital of Qingdao University, Qingdao 266003, PR China. E-mail: sunlr@vip.sina.com.
authors.forename: L-R
authors.initials: LR
authors.lastname: Sun