title: Bioluminescent Ligand-Receptor Binding Assays for Protein or Peptide Hormones.
abstract: Bioluminescence has been widely used in biomedical research due to its high sensitivity, low background, and broad linear range. In recent studies, we applied bioluminescence to ligand-receptor binding assays for some protein or peptide hormones based on a newly developed small monomeric Nanoluciferase (NanoLuc) reporter that has the so far brightest bioluminescence. The conventional ligand-receptor binding assays rely on radioligands that have drawbacks, such as radioactive hazards and short shelf lives. In contrast, the novel bioluminescent binding assays use the NanoLuc-based protein or peptide tracers that are safe, stable, and ultrasensitive. Thus, the novel bioluminescent ligand-receptor binding assay would be applied to more and more protein or peptide hormones for ligand-receptor interaction studies in future. In the present article, we provided detailed protocols for setting up the novel bioluminescent ligand-receptor binding assays using two representative protein hormones as examples.
authors.affiliation: Research Center for Translational Medicine at East Hospital, College of Life Sciences and Technology, Tongji University, Shanghai, China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Research Center for Translational Medicine at East Hospital, College of Life Sciences and Technology, Tongji University, Shanghai, China. zhan-yun.guo@tongji.edu.cn.
authors.forename: Zhan-Yun
authors.initials: ZY
authors.lastname: Guo
mesh_terms: D001667:Binding, Competitive; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D049408:Luminescent Agents; D008163:Luminescent Measurements / Q000379:methods*; D036361:Peptide Hormones / Q000378:metabolism*; D011485:Protein Binding; D018000:Receptors, Peptide / Q000378:metabolism*
chemical_list: D008024:Ligands; D049408:Luminescent Agents; D036361:Peptide Hormones; D018000:Receptors, Peptide; D008156:Luciferases
keywords: Binding; Bioluminescence; Ligand; Peptide; Protein; Receptor; Tracer