title: [Induction of rat hepatic CYP2E1 expression by arecoline in vivo].
abstract: The regulation mechanism of arecoline on rat hepatic CYP2E1 was studied in vivo. After oral administration of arecoline hydrobromide (AH; 4, 20 and 100 mg x kg(-1) x d(-1)) to rats for one week, the hepatic CYP2E1 mRNA level remained unchanged, but the hepatic CYP2E1 protein content was dose-dependently increased. Additionally, although the hepatic CYP2E1 activity was induced by AH treatment, the induction was attenuated with the increase in dosage. The results indicate that the effect of arecoline on rat hepaticdoes not involve transcriptional activation of the gene, but largely involves the stabilization of CYP2E1 protein against degradation or increased efficiency of CYP2E1 mRNA translation, and additionally involve the post- ranslational modification of CYP2E1 protein. Furthermore, the CYP2E1 response is fairly equal among the different species, the induction of rat hepatic CYP2E1 by arecoline suggests that there is a risk of metabolic interaction among the substrate drugs of CYP2E1 in betel-quid use human.
authors.forename: Xiang-tao
authors.initials: XT
authors.lastname: Huang
authors.forename: Run-mei
authors.initials: RM
authors.lastname: Xiao
authors.forename: Ming-feng
authors.initials: MF
authors.lastname: Wang
authors.forename: Jun-jun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D001115:Arecoline / Q000494:pharmacology*; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D065700:Cytochrome P-450 CYP2E1 Inducers / Q000494:pharmacology*; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger; D051381:Rats
chemical_list: D065700:Cytochrome P-450 CYP2E1 Inducers; D012333:RNA, Messenger; D001115:Arecoline; D019392:Cytochrome P-450 CYP2E1