title: Green synthesis of nano zinc oxide and evaluation of its impact on germination and metabolic activity of Solanum lycopersicum.
abstract: In the present study, zinc oxide nanoparticles (ZnO NPs) were rapidly synthesized at room temperature by treating zinc acetate dihydrate with the flower extract of Elaeagnus angustifolia (Russian olive). The formation of ZnO NPs was primarily confirmed by UV-visible absorption spectrum in the range of 250-700nm. XRD analysis and DLS particle size analyzer revealed the size of ZnO NPs. The FTIR spectrum revealed the presence of phytochemicals in the flower extract mediated ZnO NPs. Moreover, the morphology of the ZnO NPs was determined using SEM. Seeds of Solanum lycopersicum (tomato) were separately treated with different concentrations of synthesized ZnO NPs and zinc sulphate (ZnSO4) salt suspensions (common zinc supplement). The effect of these treatments on seed germination, seedling vigor, chlorophyll, protein and sugar contents as well as on the activities of lipid peroxidation and antioxidant enzyme were studied. Leaves of plants treated with 6.1mM concentration of ZnO NPs recorded maximum reflectance while it was minimum in plants treated with 1.2mM concentration of NPs. The effect of synthesized ZnO NPs on seedling vigor, pigment, protein and sugar content was found affirmative at lower concentrations contrary to control and ZnSO4 salt. The inhibitory effect at higher concentration of NPs indicated importance in the precise application of NPs, in Zn deficient system, where plant response varies with concentration. To the best of our knowledge this is the first report on Elaeagnus angustifolia mediated synthesis of ZnO NPs and their effects on germination and physiological activity of tomato.
authors.affiliation: Plant Physiology Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, U.P., India.
authors.forename: Ajey
authors.initials: A
authors.lastname: Singh
authors.affiliation: Plant Physiology Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, U.P., India. Electronic address: singhnb166@gmail.com.
authors.forename: N B
authors.initials: NB
authors.lastname: Singh
authors.affiliation: Plant Physiology Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, U.P., India.
authors.forename: Imtiyaz
authors.initials: I
authors.lastname: Hussain
authors.affiliation: Plant Physiology Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, U.P., India.
authors.forename: Himani
authors.initials: H
authors.lastname: Singh
authors.affiliation: Plant Physiology Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, U.P., India.
authors.forename: Vijaya
authors.initials: V
authors.lastname: Yadav
authors.affiliation: Laser Spectroscopy Laboratory, Department of Physics, University of Allahabad, Allahabad 211002, U.P., India.
authors.forename: S C
authors.initials: SC
authors.lastname: Singh
mesh_terms: D031263:Elaeagnaceae / Q000737:chemistry; D035264:Flowers / Q000737:chemistry; D018525:Germination / Q000187:drug effects*; D055772:Green Chemistry Technology; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D012639:Seeds / Q000187:drug effects; D015034:Zinc Oxide / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D015034:Zinc Oxide
keywords: Elaeagnus angustifolia; Flower extract; Nanoparticles; Precise application; Reflectance; Solanum lycopersicum