title: The Effect of Resveratrol and Quercetin Treatment on PPAR Mediated Uncoupling Protein (UCP-) 1, 2, and 3 Expression in Visceral White Adipose Tissue from Metabolic Syndrome Rats.
abstract: Uncoupling proteins (UCPs) are members of the mitochondrial anion carrier superfamily involved in the control of body temperature and energy balance regulation. They are currently proposed as therapeutic targets for treating obesity and metabolic syndrome (MetS). We studied the gene expression regulation of UCP1, -2, and -3 in abdominal white adipose tissue (WAT) from control and MetS rats treated with two doses of a commercial mixture of resveratrol (RSV) and quercetin (QRC). We found that UCP2 was the predominantly expressed isoform, UCP3 was present at very low levels, and UCP1 was undetectable. The treatment with RSV + QRC did not modify UCP3 levels; however, it significantly increased UCP2 mRNA in control and MetS rats in association with an increase in oleic and linoleic fatty acids. WAT from MetS rats showed a significantly increased expression of peroxisome proliferator-activated receptor (PPAR)-α and PPAR-γ when compared to the control group. Furthermore, PPAR-α protein levels were increased by the highest dose of RSV + QRC in the control and MetS groups. PPAR-γ expression was only increased in the control group. We conclude that the RSV + QRC treatment leads to overexpression of UCP2, which is associated with an increase in MUFA and PUFA, which might increase PPAR-α expression.
authors.affiliation: Department of Physiology, Instituto Nacional de Cardiolog&#237;a "Ignacio Ch&#225;vez", Juan Badiano 1, Tlalpan, Mexico City 14080, Mexico. vcastrejn@yahoo.com.mx.
authors.forename: Vicente
authors.initials: V
authors.lastname: Castrejón-Tellez
authors.affiliation: Department of Molecular Biology, Instituto Nacional de Cardiolog&#237;a "Ignacio Ch&#225;vez", Juan Badiano 1, Tlalpan, Mexico City 14080, Mexico. josemanuel_rodriguezperez@yahoo.com.mx.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Rodríguez-Pérez
authors.affiliation: Department of Pathology, Instituto Nacional de Cardiolog&#237;a "Ignacio Ch&#225;vez", Juan Badiano 1, Tlalpan, Mexico City 14080, Mexico. pertorisr@yahoo.com.mx.
authors.forename: Israel
authors.initials: I
authors.lastname: Pérez-Torres
authors.affiliation: Department of Molecular Biology, Instituto Nacional de Cardiolog&#237;a "Ignacio Ch&#225;vez", Juan Badiano 1, Tlalpan, Mexico City 14080, Mexico. unicanona@yahoo.com.mx.
authors.forename: Nonanzit
authors.initials: N
authors.lastname: Pérez-Hernández
authors.affiliation: Department of Immunology Research, Instituto Nacional de Enfermedades Respiratorias "Ismael Cos&#237;o Villegas", Calzada de Tlalpan 4502, Tlalpan, Mexico City 14080, Mexico. alfredoc1@gmail.com.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Cruz-Lagunas
authors.affiliation: Department of Physiology, Instituto Nacional de Cardiolog&#237;a "Ignacio Ch&#225;vez", Juan Badiano 1, Tlalpan, Mexico City 14080, Mexico. gualanv@yahoo.com.
authors.forename: Verónica
authors.initials: V
authors.lastname: Guarner-Lans
authors.affiliation: Department of Molecular Biology, Instituto Nacional de Cardiolog&#237;a "Ignacio Ch&#225;vez", Juan Badiano 1, Tlalpan, Mexico City 14080, Mexico. gvargas63@yahoo.com.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Vargas-Alarcón
authors.affiliation: Department of Physiology, Instituto Nacional de Cardiolog&#237;a "Ignacio Ch&#225;vez", Juan Badiano 1, Tlalpan, Mexico City 14080, Mexico. esther_rubio_ruiz@yahoo.com.
authors.forename: María Esther
authors.initials: ME
authors.lastname: Rubio-Ruiz
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects*; D007328:Insulin / Q000097:blood; D019787:Linoleic Acid / Q000378:metabolism; D008297:Male; D024821:Metabolic Syndrome / Q000188:drug therapy* / Q000473:pathology / Q000662:veterinary; D000071245:Mitochondrial Uncoupling Proteins / Q000235:genetics* / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism*; D011794:Quercetin / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use*; D000071256:Uncoupling Protein 1 / Q000235:genetics / Q000378:metabolism; D000071246:Uncoupling Protein 2 / Q000235:genetics / Q000378:metabolism; D000071247:Uncoupling Protein 3 / Q000235:genetics / Q000378:metabolism
chemical_list: D007328:Insulin; D000071245:Mitochondrial Uncoupling Proteins; D047492:Peroxisome Proliferator-Activated Receptors; D012333:RNA, Messenger; D013267:Stilbenes; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3; D019301:Oleic Acid; D011794:Quercetin; D019787:Linoleic Acid; D000077185:Resveratrol
keywords: metabolic syndrome; obesity; quercetin; resveratrol; uncoupling proteins