title: The Effect of Adipose-Derived Stem Cells on Full-Thickness Skin Grafts.
abstract: Background. The purpose of this study was to evaluate the effects of ASCs on full-thickness skin grafts. Specifically, we investigated the anti-inflammatory effects of ASCs that are mediated via regulation of the phenotypes of activated macrophages. Methods. ASCs were isolated, cultured, and injected under full-thickness skin grafts in 15 rats (ASC group). An additional 15 rats served as controls (PBS group). Skin graft survival assessment and vascularization detection were assessed with H&E staining and laser Doppler blood flowmetry (LDF). The effects of ASCs on angiogenesis, anti-inflammation, collagen accumulation-promoting, and antiscarring were assessed. Results. We found that the skin graft survival rate was significantly increased in the ASC group. The neovascularization, collagen deposition, collagen type I to type III ratio, and levels of VEGF and TGF-β3 in the ASC group were markedly higher than those in the PBS group at day 14. Additionally, in the ASC group, the levels of iNOS, IL-1β, and TNF-α were remarkably decreased, whereas the levels of IL-10 and Arg-1 were substantially increased. Conclusions. Our results confirm that ASCs transplantation can effectively improve full-thickness skin graft survival. Additionally, the anti-inflammatory role of ASCs may indirectly contribute to skin graft survival via its effect on macrophage polarization.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Chinese PLA General Hospital, Beijing 100853, China; Medicine School of Chinese PLA, Beijing 100853, China.
authors.forename: Juan
authors.identifier: 0000-0001-9220-3582
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Haojie
authors.initials: H
authors.lastname: Hao
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China; School of Medicine, Nankai University, Tianjin 300071, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Deyun
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Plastic and Reconstructive Surgery, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Han
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D000818:Animals; D002454:Cell Differentiation; D016207:Cytokines / Q000276:immunology*; D006085:Graft Survival / Q000276:immunology*; D007155:Immunologic Factors / Q000276:immunology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000276:immunology* / Q000473:pathology; D016038:Skin Transplantation / Q000379:methods*; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000276:immunology; D016896:Treatment Outcome
chemical_list: D016207:Cytokines; D007155:Immunologic Factors