title: Direct evidence of specific localization of sesquiterpenes and marchantin A in oil body cells of Marchantia polymorpha L.
abstract: Liverworts are a rich source of a diverse array of specialized metabolites, such as terpenoids and benzenoids, which are potentially useful for pharmaceutical or agrochemical applications, and also provide clues to elucidate the strategy by which liverworts adapt to the terrestrial environment. Liverworts, belonging to orders Marchantiales and Jungermanniales, possess oil bodies. In Marchantia polymorpha L., oil bodies are confined to scattered idioblastic oil body cells. It has been assumed that the specialized metabolites in M. polymorpha specifically accumulate in the oil bodies in oil body cells; however, no direct evidence was previously available for this specific accumulation. In this study, direct evidence was obtained using micromanipulation techniques coupled with MS analysis that demonstrated the specific accumulation of sesquiterpenoids and marchantin A in the oil body cells of M. polymorpha thalli. It was also observed that the number of oil body cells increased in thalli grown in low-mineral conditions. The amounts of sesquiterpenoids and marchantin A detected in crude extract prepared from the whole thallus were roughly proportional to the number of oil body cells found in a given volume of thallus, suggesting that oil body cell differentiation and sesquiterpenoid and marchantin A biosynthetic pathways are coordinated with each other.
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture and Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: mytnk.a424@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan. Electronic address: tsuyoshi-esaki@nibiohn.go.jp.
authors.forename: T
authors.initials: T
authors.lastname: Esaki
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan. Electronic address: kenmoku@ph.bunri-u.ac.jp.
authors.forename: H
authors.initials: H
authors.lastname: Kenmoku
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture and Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: takaori@yamaguchi-u.ac.jp.
authors.forename: T
authors.initials: T
authors.lastname: Koeduka
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture and Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: s018ge@yamaguchi-u.ac.jp.
authors.forename: Y
authors.initials: Y
authors.lastname: Kiyoyama
authors.affiliation: Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan. Electronic address: masujima@riken.jp.
authors.forename: T
authors.initials: T
authors.lastname: Masujima
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan. Electronic address: asakawa@ph.bunri-u.ac.jp.
authors.forename: Y
authors.initials: Y
authors.lastname: Asakawa
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture and Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: matsui@yamaguchi-u.ac.jp.
authors.forename: K
authors.initials: K
authors.lastname: Matsui
mesh_terms: D001632:Bibenzyls / Q000737:chemistry / Q000302:isolation & purification*; D004988:Ethers, Cyclic / Q000737:chemistry / Q000302:isolation & purification*; D066292:Lipid Droplets; D047008:Marchantia / Q000737:chemistry*; D015394:Molecular Structure; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D001632:Bibenzyls; D004988:Ethers, Cyclic; D012717:Sesquiterpenes; C096123:marchantin A
keywords: Common liverwort; Marchantia polymorpha; Marchantiaceae; Marchantin A (PubChem CID:442710); Marchantins; Oil body; Oil body cell; Sesquiterpenoids; Thujopsene (CID: 97829); β-Chamigrene (CID: 442353)