title: Visible-Light Optical Coherence Tomography Angiography for Monitoring Laser-Induced Choroidal Neovascularization in Mice.
abstract: This study sought to determine the earliest time-point at which evidence of choroidal neovascularization (CNV) could be detected with visible-light optical coherence tomography angiography (vis-OCTA) in a mouse model of laser-induced CNV.
  
  
  
  Visible light-OCTA was used to study laser-induced CNV at different time-points after laser injury to monitor CNV development and measure CNV lesion size. Measurements obtained from vis-OCTA angiograms were compared with histopathologic measurements from isolectin-stained choroidal flatmounts.
  
  
  
  Choroidal neovascularization area measurements between the vis-OCTA system and isolectin-stained choroidal flatmounts were significantly different in area for days 2 to 4 postlaser injury, and were not significantly different in area for days 5, 7, and 14. Choroidal neovascularization area measurements taken from the stained flatmounts were larger than their vis-OCTA counterparts for all time-points. Both modalities showed a similar trend of CNV size increasing from the day of laser injury until a peak of day 7 postlaser injury and subsequently decreasing by day 14.
  
  
  
  The earliest vis-OCTA can detect the presence of aberrant vessels in a mouse laser-induced CNV model is 5 days after laser injury. Visible light-OCTA was able to visualize the maximum of the CNV network 7 days postlaser injury, in accordance with choroidal flatmount immunostaining. Visible light-OCTA is a reliable tool in both detecting the presence of CNV development, as well as accurately determining the size of the lesion in a mouse laser-induced CNV model.
authors.affiliation: Department of Ophthalmology Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States.
authors.forename: Ronil S
authors.initials: RS
authors.lastname: Shah
authors.affiliation: Department of Ophthalmology Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States 2Functional Optical Imaging Laboratory, Department of Biomedical Engineering, Northwestern University, Chicago, Illinois, United States 3Med.
authors.forename: Brian T
authors.initials: BT
authors.lastname: Soetikno
authors.affiliation: Functional Optical Imaging Laboratory, Department of Biomedical Engineering, Northwestern University, Chicago, Illinois, United States.
authors.forename: Ji
authors.initials: J
authors.lastname: Yi
authors.affiliation: Functional Optical Imaging Laboratory, Department of Biomedical Engineering, Northwestern University, Chicago, Illinois, United States.
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Skondra
authors.affiliation: Department of Ophthalmology Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States 2Functional Optical Imaging Laboratory, Department of Biomedical Engineering, Northwestern University, Chicago, Illinois, United States.
authors.forename: Hao F
authors.initials: HF
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States.
authors.forename: Amani A
authors.initials: AA
authors.lastname: Fawzi
mesh_terms: D000818:Animals; D002829:Choroid / Q000098:blood supply* / Q000473:pathology; D020256:Choroidal Neovascularization / Q000175:diagnosis*; D004195:Disease Models, Animal; D005451:Fluorescein Angiography / Q000379:methods*; D005654:Fundus Oculi; D017075:Laser Coagulation / Q000009:adverse effects; D008027:Light; D051379:Mice; D008810:Mice, Inbred C57BL; D041623:Tomography, Optical Coherence / Q000379:methods*