title: Development of a bench-top extra-cleanroom for DNA amplification.
abstract: Prevention of airborne contamination has become an important factor in biotechnology; however, conventional laminar-airflow cabinets (LAF-cabinets) are no longer sufficient as a countermeasure against nano-sized airborne contaminants in the laboratory. Here we present a bench-top extra-cleanroom classified as ISO-1 that can prevent contamination from airborne nanoparticles. This bench-top extra-cleanroom consists of a novel clean-zone-creating system that is equipped with nanofibrous, nonwoven filters. In addition, the cleanroom is also equipped with an ionizer to prevent plasticware from collecting dust by electrostatic charge attraction. This combination of features allows the cleanroom to prevent DNA contamination derived from airborne nanoparticles. Our extra-cleanroom with ionizer could be useful in various areas of biotechnology that are easily affected by airborne contaminants.
authors.affiliation: Graduate School of Advanced Sciences of Matter, Hiroshima University, Hiroshima, Japan.|CREST, Japan Science and Technology Agency, Hiroshima, Japan.|NanoBiotetcnology Laboratory, Food Engineering Division, National Food Research Institute, National Agriculture and Food Research Organization, Ibaraki, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Environment Engineering Division, Koken Ltd, Tokyo, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Satoh
authors.affiliation: NanoBiotetcnology Laboratory, Food Engineering Division, National Food Research Institute, National Agriculture and Food Research Organization, Ibaraki, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kanahara
authors.affiliation: Hanno Laboratory, Koken Ltd, Saitama, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kubota
authors.affiliation: NanoBiotetcnology Laboratory, Food Engineering Division, National Food Research Institute, National Agriculture and Food Research Organization, Ibaraki, Japan.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Hirose
authors.affiliation: Isehara Research Laboratory, Technology & Development Division, Kanto Chemical Co., Inc, Isehara, Kanagawa, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamazaki
authors.affiliation: Insect Genome Laboratory, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Graduate School of Advanced Sciences of Matter, Hiroshima University, Hiroshima, Japan.|CREST, Japan Science and Technology Agency, Hiroshima, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Okamura
authors.affiliation: Environment Engineering Division, Koken Ltd, Tokyo, Japan.
authors.forename: Taketo
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: NanoBiotetcnology Laboratory, Food Engineering Division, National Food Research Institute, National Agriculture and Food Research Organization, Ibaraki, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Kobori
mesh_terms: D004247:DNA / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D004340:Drug Contamination / Q000517:prevention & control; D004587:Electrophoresis, Agar Gel; D004780:Environment, Controlled; D004866:Equipment Contamination / Q000517:prevention & control; D004867:Equipment Design; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation* / Q000379:methods*
chemical_list: D004247:DNA
keywords: airborne nanoparticle contamination; bench-top extra-cleanroom; random RNA primers; whole-genome amplification; ø29 DNA polymerase