title: Characterization of morphology and resistance to Blumeria graminis of winter triticale monosomic addition lines with chromosome 2D of Aegilops tauschii.
abstract: Allocation of the chromosome 2D of Ae. tauschii in triticale background resulted in changes of its organization, what is related to varied expression of genes determining agronomically important traits. Monosomic alien addition lines (MAALs) are crucial for transfer of genes from wild relatives into cultivated varieties. This kind of genetic stocks is used for physical mapping of specific chromosomes and analyzing alien genes expression. The main aim of our study is to improve hexaploid triticale by transferring D-genome chromatin from Aegilops tauschii × Secale cereale (2n = 4x = 28, DDRR). In this paper, we demonstrate the molecular cytogenetics analysis and SSR markers screening combined with phenotype analysis and evaluation of powdery mildew infection of triticale monosomic addition lines carrying chromosome 2D of Ae. tauschii. We confirmed the inheritance of chromosome 2D from the BC2F4 to the BC2F6 generation of triticale hybrids. Moreover, we unveiled a high variable region on the short arm of chromosome 2D, where chromosome rearrangements were mapped. These events had direct influence on plant height of hybrids what might be connected with changes at Rht8 loci. We obtained 20 semi-dwarf plants of BC2F6 generation carrying 2D chromosome with the powdery mildew resistance, without changes in spike morphology, which can be used in the triticale breeding programs.
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland. mmaj@igr.poznan.pl.
authors.forename: M
authors.initials: M
authors.lastname: Majka
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kwiatek
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Belter
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland.
authors.forename: H
authors.initials: H
authors.lastname: Wiśniewska
mesh_terms: D001203:Ascomycota / Q000502:physiology*; D002843:Chromatin / Q000378:metabolism; D032461:Chromosomes, Plant / Q000235:genetics*; D003433:Crosses, Genetic; D060467:Disease Resistance / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005819:Genetic Markers; D018745:Genome, Plant; D006824:Hybridization, Genetic; D007178:Inbreeding; D007621:Karyotyping; D018895:Microsatellite Repeats / Q000235:genetics; D008938:Mitosis / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology*; D006109:Poaceae / Q000235:genetics*; D000066889:Triticale / Q000033:anatomy & histology* / Q000382:microbiology*
chemical_list: D002843:Chromatin; D005819:Genetic Markers
keywords: Aegilops; In situ hybridization; Monosomic alien addition lines; Plant height; Powdery mildew; Triticale