title: Regulation of Intestinal Epithelial Calcium Transport Proteins by Stanniocalcin-1 in Caco2 Cells.
abstract: Stanniocalcin-1 (STC1) is a calcium and phosphate regulatory hormone. However, the exact molecular mechanisms underlying how STC1 affects Ca(2+) uptake remain unclear. Here, the expression levels of the calcium transport proteins involved in transcellular transport in Caco2 cells were examined following over-expression or inhibition of STC1. These proteins include the transient receptor potential vanilloid members (TRPV) 5 and 6, the plasma membrane calcium ATPase 1b (PMCA1b), the sodium/calcium exchanger (NCX1), and the vitamin D receptor (VDR). Both gene and protein expressions of TRPV5 and TRPV6 were attenuated in response to over-expression of STC1, and the opposite trend was observed in cells treated with siRNASTC1. To further investigate the ability of STC1 to influence TRPV6 expression, cells were treated with 100 ng/mL of recombinant human STC1 (rhSTC1) for 4 h following pre-transfection with siRNASTC1 for 48 h. Intriguingly, the increase in the expression of TRPV6 resulting from siRNASTC1 was reversed by rhSTC1. No significant effect of STC1 on the expression of PMCA1b, NCX1 or VDR was observed in this study. In conclusion, the effect of STC1 on calcium transport in intestinal epithelia is due to, at least in part, its negative regulation of the epithelial channels TRPV5/6 that mediate calcium influx.
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China. hbswkjxjm@163.com.|Department of Animal Science, Hubei Vocational College Of Bio-Technology, Wuhan 430070, Hubei, China. hbswkjxjm@163.com.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Hubei Key Laboratory of Embryo and Molecular Breeding, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei, China. hbnkyguorui@163.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China. wanchunyun16@163.com.|College of Animal Science, Yangtze University, Jingzhou 434023, Hubei, China. wanchunyun16@163.com.
authors.forename: Chunyun
authors.initials: C
authors.lastname: Wan
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China. hnzdwlm@163.com.
authors.forename: Liming
authors.initials: L
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China. hznydxysj@163.com.
authors.forename: Shijin
authors.initials: S
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China. hlgdz@163.com.
authors.forename: Dingzong
authors.initials: D
authors.lastname: Guo
mesh_terms: D018938:Caco-2 Cells; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006023:Glycoproteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D019831:Sodium-Calcium Exchanger / Q000235:genetics / Q000378:metabolism; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism
chemical_list: D015220:Calcium Channels; D006023:Glycoproteins; D034741:RNA, Small Interfering; D018167:Receptors, Calcitriol; D011994:Recombinant Proteins; D019831:Sodium-Calcium Exchanger; D050916:TRPV Cation Channels; C492239:TRPV5 protein, human; C422827:TRPV6 protein, human; C110374:sodium-calcium exchanger 1; C051795:teleocalcin; D002118:Calcium
keywords: TRPV5; TRPV6; calcium; intestinal epithelium; stanniocalcin-1