title: Analysis on transglutaminase 1 and its substrates using specific substrate peptide in cultured keratinocytes.
abstract: Transglutaminase (TGase) catalyzes protein cross-linking reactions essential for several biological processes. In differentiating keratinocytes, TG1 (keratinocyte-type) is crucial for the cross-linking of substrate proteins required for the complete formation of the cornified envelop, a proteinaceous supermolecule located in the outermost layer of the epidermis. TG1 expressions and its substrate were induced in cultured keratinocytes at differentiation-stage specific manner. In the cultured keratinocytes, we used the TG1-specific substrate peptide, which enables the specific detection of enzymatic activity to investigate its induction patterns. As a further application of the substrate peptide, several substrate candidates of TG1 that may be essential for cornified envelope formation were identified and characterized.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa, Furoh-cho, Nagoya, 464-8601, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Yamane
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa, Furoh-cho, Nagoya, 464-8601, Japan.
authors.forename: Kayoko
authors.initials: K
authors.lastname: Sugimura
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa, Furoh-cho, Nagoya, 464-8601, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kawasaki
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa, Furoh-cho, Nagoya, 464-8601, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Tatsukawa
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa, Furoh-cho, Nagoya, 464-8601, Japan. Electronic address: hitomi@ps.nagoya-u.ac.jp.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Hitomi
mesh_terms: D002478:Cells, Cultured; D004789:Enzyme Activation; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology*; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D013379:Substrate Specificity; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D010455:Peptides; D011503:Transglutaminases; C105804:transglutaminase 1
keywords: Epidermis; Keratinocyte; Transglutaminase