title: Expression of aquaporins 1 and 5 in a model of ventilator-induced lung injury and its relation to tidal volume.
abstract: What is the central question of this study? Although different studies have attempted to find factors that influence the expression of aquaporins (AQPs) in the lung in different situations, to date no research group has explored the expression of AQP1 and AQP5 jointly in rats mechanically ventilated with different tidal volumes in a model of ventilator-induced lung injury. What is the main finding? Mechanical ventilation with a high tidal volume causes lung injury and oedema, increasing lung permeability. In rats ventilated with a high tidal volume, the pulmonary expression of AQP1 decreases. We analysed the expression of aquaporins 1 and 5 and its relation with tidal volume in a model of ventilator-induced lung injury. Forty-two rats were used. Six non-ventilated animals were killed (control group). The remaining rats were ventilated for 2 h with different tidal volumes (group 7ML with 7 ml kg-1and group 20ML with 20 ml kg-1) and a respiratory rate of 90 breaths min-1. Lung oedema was measured, and the expression of AQP1 and AQP5 was determined by Western immunoblotting and measurement of mRNA. Lung oedema and alveolar-capillary membrane permeability were significantly increased in the animals of group 20ML compared with the control group. Expression of AQP1 was decreased in groups 7ML and 20ML compared with the control group. In conclusion, mechanical ventilation with a high tidal volume causes lung injury and oedema, increasing lung permeability. In rats ventilated with a high tidal volume, the pulmonary expression of AQP1 decreases.
authors.affiliation: Hospital General Universitario, Valencia, Spain. gfabregat@gmail.com.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Fabregat
authors.affiliation: Hospital Clinico Universitario, Valencia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: García-de-la-Asunción
authors.affiliation: School of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Benjamín
authors.initials: B
authors.lastname: Sarriá
authors.affiliation: School of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Mata
authors.affiliation: School of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Julio
authors.initials: J
authors.lastname: Cortijo
authors.affiliation: Hospital General Universitario, Valencia, Spain.|School of Medicine, University of Valencia, Valencia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: de Andrés
authors.affiliation: Hospital Universitario Miguel Servet, Zaragoza, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Gallego
authors.affiliation: Hospital Clinico Universitario, Valencia, Spain.|School of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Belda
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism*; D051402:Aquaporin 5 / Q000378:metabolism*; D002199:Capillary Permeability / Q000502:physiology; D008168:Lung / Q000378:metabolism; D011654:Pulmonary Edema / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012121:Respiration, Artificial / Q000379:methods; D013990:Tidal Volume / Q000502:physiology*; D055397:Ventilator-Induced Lung Injury / Q000378:metabolism*
chemical_list: C496022:Aqp1 protein, rat; C496040:Aqp5 protein, rat; D051402:Aquaporin 5; D012333:RNA, Messenger; D051398:Aquaporin 1
keywords: aquaporins; protein expression; ventilator induced lung injury