title: Lactobacillus fermentum CRL1446 Ameliorates Oxidative and Metabolic Parameters by Increasing Intestinal Feruloyl Esterase Activity and Modulating Microbiota in Caloric-Restricted Mice.
abstract: The purpose of this study was to determine whether the administration of the feruloyl esterase (FE)-producing strain Lactobacillus fermentum CRL1446 enhances metabolic and oxidative parameters in caloric-restricted (CR) mice. Balb/c male mice were divided into ad libitum fed Group (ALF Group), CR diet Group (CR Group) and CR diet plus L. fermentum Group (CR-Lf Group). CR diet was administered during 45 days and CRL1446 strain was given in the dose of 10⁸ cells/mL/day/mouse. FE activity was determined in intestinal mucosa and content at Day 1, 20 and 45. Triglyceride, total cholesterol, glucose, thiobarbituric acid reactive substances (TBARS) levels and glutathione reductase activity were determined in plasma. Gut microbiota was evaluated by high-throughput sequencing of 16S rRNA gene amplicons. At Day 45, total intestinal FE activity in CR-Lf Group was higher (p = 0.020) than in CR and ALF groups and an improvement in both metabolic (reductions in triglyceride (p = 0.0025), total cholesterol (p = 0.005) and glucose (p < 0.0001) levels) and oxidative (decrease of TBARS levels and increase of plasmatic glutathione reductase activity (p = 0.006)) parameters was observed, compared to ALF Group. CR diet increased abundance of Bacteroidetes and CRL1446 administration increased abundance of Bifidobacterium and Lactobacillus genus. L. fermentun CRL1446 exerted a bifidogenic effect under CR conditions.
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, San Miguel de Tucum&#225;n T4000ILC, Argentina. mrusso@cerela.org.ar.
authors.forename: Matias
authors.initials: M
authors.lastname: Russo
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, San Miguel de Tucum&#225;n T4000ILC, Argentina. faber@cerela.org.ar.
authors.forename: Emanuel
authors.initials: E
authors.lastname: Fabersani
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, San Miguel de Tucum&#225;n T4000ILC, Argentina. cabeijon@cerela.org.ar.|Facultad de Ciencias de la Salud, Universidad del Norte Santo Tom&#225;s de Aquino, San Miguel de Tucum&#225;n T4000IHC, Argentina. cabeijon@cerela.org.ar.
authors.forename: María C
authors.initials: MC
authors.lastname: Abeijón-Mukdsi
authors.affiliation: Facultad de Ciencias de la Salud, Universidad del Norte Santo Tom&#225;s de Aquino, San Miguel de Tucum&#225;n T4000IHC, Argentina. gross@fbqf.unt.edu.ar.|Universidad Nacional de Tucum&#225;n, Facultad de Bioqu&#237;mica, Qu&#237;mica y Farmacia, Ayacucho 471, San Miguel de Tucum&#225;n T4000INI, Argentina. gross@fbqf.unt.edu.ar.
authors.forename: Romina
authors.initials: R
authors.lastname: Ross
authors.affiliation: Instituto Nacional de Tecnolog&#237;a Agropecuaria INTA-EEA, Ruta Provincial 301 Km 32, Famaill&#225; 4132, Argentina. fontana.cecilia@inta.gob.ar.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Fontana
authors.affiliation: Microbial Ecology, Nutrition &amp; Health Laboratory, Agrochemistry and Food Technology Institute (IATA-CSIC), Paterna-Valencia 46980, Spain. abenitez@iata.csic.es.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Benítez-Páez
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, San Miguel de Tucum&#225;n T4000ILC, Argentina. pgauffin@cerela.org.ar.|Facultad de Ciencias de la Salud, Universidad del Norte Santo Tom&#225;s de Aquino, San Miguel de Tucum&#225;n T4000IHC, Argentina. pgauffin@cerela.org.ar.
authors.forename: Paola
authors.initials: P
authors.lastname: Gauffin-Cano
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, San Miguel de Tucum&#225;n T4000ILC, Argentina. rmedina@cerela.org.ar.|Universidad Nacional de Tucum&#225;n, Facultad de Bioqu&#237;mica, Qu&#237;mica y Farmacia, Ayacucho 471, San Miguel de Tucum&#225;n T4000INI, Argentina. rmedina@cerela.org.ar.
authors.forename: Roxana B
authors.initials: RB
authors.lastname: Medina
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D031204:Caloric Restriction; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D000069196:Gastrointestinal Microbiome; D007422:Intestines / Q000201:enzymology* / Q000382:microbiology*; D052197:Limosilactobacillus fermentum / Q000201:enzymology*; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood; D008297:Male; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D018384:Oxidative Stress; D019936:Probiotics; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D015415:Biomarkers; D001786:Blood Glucose; D008055:Lipids; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase
keywords: Lactobacillus fermentum; caloric restriction diet; intestinal feruloyl esterase