title: Flow Preconditioning of Endothelial Cells on Collagen-Immobilized Silicone Fibers Enhances Cell Retention and Antithrombotic Function.
abstract: Stability and antithrombotic functionality of endothelial cells on silicone hollow fibers (SiHFs) are critical in the development of biohybrid artificial lungs. Here we aimed to enhance endothelial cell retention and anti-thrombotic function by low (12 dyn/cm2, 24 h) fluid shear stress ("flow") preconditioning of endothelial cells seeded on collagen-immobilized SiHFs. The response of endothelial cells without preconditioning (48 h static culture) and with preconditioning (24 h static culture followed by 24 h flow preconditioning) on hollow fibers to high fluid shear stress (30 dyn/cm2, 1 h) was assessed in a parallel-plate flow chamber. Finite element (FE) modeling was used to simulate shear stress within the flow chamber. We found that collagen immobilization on hollow fibers using carbodiimide bonds provided sufficient stability to high shear stress. Flow preconditioning for 24 h before treatment with high shear stress for 1 h on collagen-immobilized hollow fibers increased cell retention (1.3-fold). The FE model showed that cell flattening due to flow preconditioning reduced maximum shear stress on cells by 32%. Flow preconditioning prior to exposure to high fluid shear stress enhanced the production of nitric oxide (1.3-fold) and prostaglandin I2(1.2-fold). In conclusion, flow preconditioning of endothelial cells on collagen-immobilized SiHFs enhanced cell retention and antithrombotic function, which could significantly improve current biohybrid artificial lungs.
authors.affiliation: School of Chemical Engineering, College of Engineering, University of Tehran, Tehran.|Department of Biomedical Engineering, Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran.|Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Amsterdam, The Netherlands.
authors.forename: Nasim
authors.initials: N
authors.lastname: Salehi-Nik
authors.affiliation: School of Chemical Engineering, College of Engineering, University of Tehran, Tehran.|Department of Biomedical Engineering, Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran.
authors.forename: Seyedeh Parnian
authors.initials: SP
authors.lastname: Banikarimi
authors.affiliation: School of Chemical Engineering, College of Engineering, University of Tehran, Tehran.|Department of Biomedical Engineering, Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran.
authors.forename: Ghassem
authors.initials: G
authors.lastname: Amoabediny
authors.affiliation: Department of Orthopedics, University Medical Center Utrecht, Utrecht.|Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, Delft, The Netherlands.
authors.forename: Behdad
authors.initials: B
authors.lastname: Pouran
authors.affiliation: National Cell Bank, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.affiliation: Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Amsterdam, The Netherlands.
authors.forename: Behrouz
authors.initials: B
authors.lastname: Zandieh-Doulabi
authors.affiliation: Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Amsterdam, The Netherlands.
authors.forename: Jenneke
authors.initials: J
authors.lastname: Klein-Nulend
mesh_terms: D021522:Bioartificial Organs; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D004867:Equipment Design; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D057446:Hydrodynamics; D055767:Immobilized Proteins / Q000737:chemistry; D008168:Lung / Q000098:blood supply / Q000166:cytology / Q000502:physiology; D008422:Materials Testing; D009569:Nitric Oxide / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism; D012828:Silicones / Q000737:chemistry*; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods
chemical_list: D020099:Coated Materials, Biocompatible; D055767:Immobilized Proteins; D011453:Prostaglandins; D012828:Silicones; D009569:Nitric Oxide; D003094:Collagen
keywords: Antithrombotic function; Biohybrid artificial lung; Collagen immobilization; Endothelialization; Finite element modeling; Fluid shear stress