title: Sodium chloride concentration determines exoelectrogens in anode biofilms occurring from mangrove-grown brackish sediment.
abstract: Single-chamber microbial fuel cells (MFCs) were inoculated with mangrove-grown brackish sediment (MBS) and continuously supplied with an acetate medium containing different concentrations of NaCl (0-1.8M). Different from MFCs inoculated with paddy-field soil (high power outputs were observed between 0.05 and 0.1M), power outputs from MBS-MFCs were high at NaCl concentrations from 0 to 0.6M. Amplicon-sequence analyses of anode biofilms suggest that different exoelectrogens occurred from MBS depending on NaCl concentrations; Geobacter occurred abundantly below 0.1M, whereas Desulfuromonas was abundant from 0.3M to 0.6M. These results suggest that NaCl concentration is the major determinant of exoelectrogens that occur in anode biofilms from MBS. It is also suggested that MBS is a potent source of microbes for MFCs to be operated in a wide range of NaCl concentrations.
authors.affiliation: School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.
authors.forename: Morio
authors.initials: M
authors.lastname: Miyahara
authors.affiliation: School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kouzuma
authors.affiliation: School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan. Electronic address: kazuyaw@toyaku.ac.jp.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D001674:Bioelectric Energy Sources; D018441:Biofilms; D004560:Electricity; D004566:Electrodes; D045302:Geobacter / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D064307:Microbiota / Q000187:drug effects*; D054712:Salinity; D012965:Sodium Chloride / Q000008:administration & dosage*; D053833:Wetlands
chemical_list: D012965:Sodium Chloride
keywords: 16S rRNA gene; Bioelectricity; Ionic strength; Pyrosequencing; Wastewater treatment