title: Inhibitory effect of ebselen on the oxidation of low density lipoprotein.
abstract: The inhibitory effect of a synthesized glutathione peroxidase (GSHPX) mimic- ebselen, and its cofactor glutathione (GSH), on the oxidation of low density lipoprotein (LDL) induced by Cu(2+) was studied by determination of hydroperoxides and thiobarbituric acid reactive substances (TBARS). Ebselen alone had a strong inhibitory effect on the oxidation of LDL. The lag time of LDL oxidation was prolonged with an increase in the concentration of ebselen. The inhibitory effect of 5 μM ebselen was equivalent to that of 50 μM α-tocopherol. When GSH was present, ebselen exhibited stronger inhibitory effect than when present alone. With 50 μM GSH, ebselen at a concentration as low as 5 μM could inhibit oxidation of LDL induced by 5 μM Cu(2+) completely for at least 24 h. Ebselen at high concentrations (100 μM) decomposed hydroperoxides in pre-oxidized LDL and effectively prevented its further oxidation, but not in the present of EDTA. Low concentration of ebselen (5 μM) plus GSH (50 μM) decomposed hydroperoxides in pre-oxidized LDL whether EDTA was added or not.
authors.affiliation: a Free Radical Medicine Research Laboratory , First Military Medical University , Guang Zhou , P. R. China.
authors.forename: S X
authors.initials: SX
authors.lastname: Liu
authors.affiliation: a Free Radical Medicine Research Laboratory , First Military Medical University , Guang Zhou , P. R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: a Free Radical Medicine Research Laboratory , First Military Medical University , Guang Zhou , P. R. China.
authors.forename: M
authors.initials: M
authors.lastname: Zhou
authors.affiliation: a Free Radical Medicine Research Laboratory , First Military Medical University , Guang Zhou , P. R. China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Wen
authors.affiliation: a Free Radical Medicine Research Laboratory , First Military Medical University , Guang Zhou , P. R. China.
authors.forename: M J
authors.initials: MJ
authors.lastname: Sun