title: Simultaneous determination of notoginsenoside R1 and ginsenoside Re in rat plasma by ultra high performance liquid chromatography with tandem mass spectrometry and its application to a pharmacokinetic study.
abstract: A rapid and high sensitive ultra high performance liquid chromatography with tandem mass spectrometry method for the simultaneous determination of notoginsenoside R1 and ginsenoside Re in rat plasma was developed. The analytes and internal standard, digoxin, were extracted from rat plasma via protein precipitation with methanol and separated on an Phenomenex Gemini C18 column within 2 min. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring and positive ion mode. The precursor to product ion transitions monitored for notoginsenoside R1, ginsenoside Re, and internal standard were m/z 955.5→775.5, 969.6→789.1, and 803.6→283.1, respectively. The assay was validated with linear range of 1.9-380 ng/mL for notoginsenoside R1 and 0.5-100 ng/mL for ginsenoside Re. The intra- and interday precisions (RSD%) were within 8.96% for each analyte. The absolute recoveries were greater than 93% for R1 and 96% for Re. Each analyte was stable during all sample storage, preparation, and analytic procedures. The method was successfully applied to a pharmacokinetic study of Xuesaitong dispersible tablets in eight rats.
authors.affiliation: Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Dai
authors.affiliation: Department of Pharmacy Office, Zhangjiagang Hospital of Traditional Chinese Medicine, Zhangjiagang, China.
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zong
authors.affiliation: Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Shijia
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Changyin
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, China. juwz333@hotmail.com.
authors.forename: Wenzheng
authors.initials: W
authors.lastname: Ju
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D036145:Ginsenosides / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D036145:Ginsenosides; C049864:ginsenoside Re; C072936:notoginsenoside R1
keywords: Ginsenoside Re; Notoginsenoside R1; Pharmacokinetics; Xuesaitong dispersible tablet; traditional Chinese medicine