title: A comparative study of dietary curcumin, nanocurcumin, and other classical amyloid-binding dyes for labeling and imaging of amyloid plaques in brain tissue of 5×-familial Alzheimer's disease mice.
abstract: Deposition of amyloid beta protein (Aβ) is a key component in the pathogenesis of Alzheimer's disease (AD). As an anti-amyloid natural polyphenol, curcumin (Cur) has been used as a therapy for AD. Its fluorescent activity, preferential binding to Aβ, as well as structural similarities with other traditional amyloid-binding dyes, make it a promising candidate for labeling and imaging of Aβ plaques in vivo. The present study was designed to test whether dietary Cur and nanocurcumin (NC) provide more sensitivity for labeling and imaging of Aβ plaques in brain tissues from the 5×-familial AD (5×FAD) mice than the classical Aβ-binding dyes, such as Congo red and Thioflavin-S. These comparisons were made in postmortem brain tissues from the 5×FAD mice. We observed that Cur and NC labeled Aβ plaques to the same degree as Aβ-specific antibody and to a greater extent than those of the classical amyloid-binding dyes. Cur and NC also labeled Aβ plaques in 5×FAD brain tissues when injected intraperitoneally. Nanomolar concentrations of Cur or NC are sufficient for labeling and imaging of Aβ plaques in 5×FAD brain tissue. Cur and NC also labeled different types of Aβ plaques, including core, neuritic, diffuse, and burned-out, to a greater degree than other amyloid-binding dyes. Therefore, Cur and or NC can be used as an alternative to Aβ-specific antibody for labeling and imaging of Aβ plaques ex vivo and in vivo. It can provide an easy and inexpensive means of detecting Aβ-plaque load in postmortem brain tissue of animal models of AD after anti-amyloid therapy.
authors.affiliation: Field Neurosciences Institute Laboratory for Restorative Neurology, Central Michigan University, Mt. Pleasant, MI, 48859, USA. maiti1p@cmich.edu.|Program in Neuroscience, Central Michigan University, Mt. Pleasant, MI, 48859, USA. maiti1p@cmich.edu.|Department of Psychology, Central Michigan University, Mt. Pleasant, MI, 48859, USA. maiti1p@cmich.edu.|Field Neurosciences Institute, St. Mary's of Michigan, Saginaw, MI, 48604, USA. maiti1p@cmich.edu.
authors.forename: Panchanan
authors.initials: P
authors.lastname: Maiti
authors.affiliation: Field Neurosciences Institute Laboratory for Restorative Neurology, Central Michigan University, Mt. Pleasant, MI, 48859, USA.|Program in Neuroscience, Central Michigan University, Mt. Pleasant, MI, 48859, USA.
authors.forename: Tia C
authors.initials: TC
authors.lastname: Hall
authors.affiliation: Field Neurosciences Institute Laboratory for Restorative Neurology, Central Michigan University, Mt. Pleasant, MI, 48859, USA.|Program in Neuroscience, Central Michigan University, Mt. Pleasant, MI, 48859, USA.
authors.forename: Leela
authors.initials: L
authors.lastname: Paladugu
authors.affiliation: Field Neurosciences Institute Laboratory for Restorative Neurology, Central Michigan University, Mt. Pleasant, MI, 48859, USA.|Program in Neuroscience, Central Michigan University, Mt. Pleasant, MI, 48859, USA.
authors.forename: Nivya
authors.initials: N
authors.lastname: Kolli
authors.affiliation: Field Neurosciences Institute Laboratory for Restorative Neurology, Central Michigan University, Mt. Pleasant, MI, 48859, USA.|Program in Neuroscience, Central Michigan University, Mt. Pleasant, MI, 48859, USA.
authors.forename: Cameron
authors.initials: C
authors.lastname: Learman
authors.affiliation: Field Neurosciences Institute Laboratory for Restorative Neurology, Central Michigan University, Mt. Pleasant, MI, 48859, USA.|Program in Neuroscience, Central Michigan University, Mt. Pleasant, MI, 48859, USA.|College of Medicine, Central Michigan University, Mt. Pleasant, MI, 48859, USA.
authors.forename: Julien
authors.initials: J
authors.lastname: Rossignol
authors.affiliation: Field Neurosciences Institute Laboratory for Restorative Neurology, Central Michigan University, Mt. Pleasant, MI, 48859, USA. dunba1g@cmich.edu.|Program in Neuroscience, Central Michigan University, Mt. Pleasant, MI, 48859, USA. dunba1g@cmich.edu.|Department of Psychology, Central Michigan University, Mt. Pleasant, MI, 48859, USA. dunba1g@cmich.edu.|Field Neurosciences Institute, St. Mary's of Michigan, Saginaw, MI, 48604, USA. dunba1g@cmich.edu.
authors.forename: Gary L
authors.initials: GL
authors.lastname: Dunbar
mesh_terms: D000284:Administration, Oral; D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004396:Coloring Agents / Q000008:administration & dosage* / Q000032:analysis* / Q000737:chemistry; D003474:Curcumin / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D004032:Diet; D004195:Disease Models, Animal; D051379:Mice; D008822:Mice, Transgenic; D015394:Molecular Structure; D049329:Nanostructures / Q000008:administration & dosage / Q000032:analysis; D058225:Plaque, Amyloid / Q000737:chemistry / Q000378:metabolism*; D012995:Solubility
chemical_list: D016229:Amyloid beta-Peptides; D004396:Coloring Agents; D003474:Curcumin
keywords: Alzheimer's disease; Amyloid beta protein; Curcumin; Nanocurcumin; Protein labelling