title: Direct Duplex Detection: An Emerging Tool in the RNA Structure Analysis Toolbox.
abstract: While a variety of powerful tools exists for analyzing RNA structure, identifying long-range and intermolecular base-pairing interactions has remained challenging. Recently, three groups introduced a high-throughput strategy that uses psoralen-mediated crosslinking to directly identify RNA-RNA duplexes in cells. Initial application of these methods highlights the preponderance of long-range structures within and between RNA molecules and their widespread structural dynamics.
authors.affiliation: Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599-3290, USA.
authors.forename: Chase A
authors.initials: CA
authors.lastname: Weidmann
authors.affiliation: Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599-3290, USA.
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Mustoe
authors.affiliation: Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599-3290, USA. Electronic address: weeks@unc.edu.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Weeks
mesh_terms: D020029:Base Pairing; D012313:RNA / Q000032:analysis* / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D012313:RNA