title: Two-mode Analysis by High-performance Liquid Chromatography of ρ-Aminobenzoic Ethyl Ester-derivatized Monosaccharides.
abstract: ρ-Aminobenzoic ethyl ester (ABEE)-derivatized monosaccharides were separated by HPLC with a trifluoroacetic acid (TFA) solution or borate buffer as the eluent. In the case of the TFA solution, ABEE-derivatized monosaccharides of the neutral and amino sugars found in animal glycoproteins were separated in a simultaneous analysis. In the case of the borate buffer, ABEE-derivatized monosaccharides of identical molecular weights such as ABEE-Gal, -Glc, and -Man were separated as stereoisomers. Glucuronic acid and galacturonic acid were detected and separated within 8 min. The relationship between the peak areas and the amounts of ABEE-derivatized monosaccharides was linear in the range of 1 to 1000 pmol.
authors.affiliation: a Sugiyama Chemical and Industrial Laboratory , 11 Kagetori-cho, Totsuka-ku, Yokohama, Kanagawa 245 , Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yasuno
authors.affiliation: a Sugiyama Chemical and Industrial Laboratory , 11 Kagetori-cho, Totsuka-ku, Yokohama, Kanagawa 245 , Japan.|b Honen Corporation , 1-2-3 Ohtemachi, Chiyoda-ku, Tokyo 100 , Japan.
authors.forename: T
authors.initials: T
authors.lastname: Murata
authors.affiliation: a Sugiyama Chemical and Industrial Laboratory , 11 Kagetori-cho, Totsuka-ku, Yokohama, Kanagawa 245 , Japan.|b Honen Corporation , 1-2-3 Ohtemachi, Chiyoda-ku, Tokyo 100 , Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kokubo
authors.affiliation: a Sugiyama Chemical and Industrial Laboratory , 11 Kagetori-cho, Totsuka-ku, Yokohama, Kanagawa 245 , Japan.|b Honen Corporation , 1-2-3 Ohtemachi, Chiyoda-ku, Tokyo 100 , Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.affiliation: a Sugiyama Chemical and Industrial Laboratory , 11 Kagetori-cho, Totsuka-ku, Yokohama, Kanagawa 245 , Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kamei
keywords: monosaccharide analysis; potassium borate buffer; trifluoroacetic acid; ρ-aminobenzoic ethyl ester