title: Culture media optimization of Porphyridium purpureum: production potential of biomass, total lipids, arachidonic and eicosapentaenoic acid.
abstract: Porphyridium purpureum a red marine microalga is known for phycobiliproteins (PB), polyunsaturated fatty acids and sulphated exopolysaccharides. In the present study, effects of media constituents for the production of different polyunsaturated fatty acids from P. purpureum were considered using a response surface methodology (RSM). A second order polynomial was used to predict the response functions in terms of the independent variables such as the concentrations of sodium chloride, magnesium sulphate, sodium nitrate and potassium dihydrogen phosphate. The response functions were production of biomass yield, total lipid and polyunsaturated fatty acids like arachidonic acid (AA 20:4) and eicosapentaenoic acid (EPA 20:5). Results corroborated that maximum Biomass (0.95 gL(-1)) yield was at the concentrations of sodium chloride (14.89 gL(-1)), magnesium sulfate (3.93 gL(-1)) and sodium nitrate (0.96 gL(-1)) and potassium dihydrogen phosphate (0.09 gL(-1)). Optimum total lipid (17.9 % w/w) and EPA (34.6 % w/w) content was at the concentrations of sodium chloride (29.98 gL(-1)), magnesium sulfate (9.34 gL(-1)) and sodium nitrate (1.86 gL(-1)). Variation in concentration of potassium dihydrogen phosphate for both lipid (0.01gL(-1)) and EPA content (0.20 gL(-1)) was observed. The optimum conditions for biomass, total lipid, AA and EPA varied indicating their batch mode of growth and interaction effect of the salt.
authors.affiliation: Plant Cell Biotechnology Department, Central Food, Mysore, 570020 India.
authors.forename: Mysore Doddaiah
authors.initials: MD
authors.lastname: Kavitha
authors.affiliation: Department of Molecular Biology, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625021 India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Kathiresan
authors.affiliation: Grain Science Technology Department, Central Food Technological Research Institute, Mysore, 570020 India.
authors.forename: Sila
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: Plant Cell Biotechnology Department, Central Food, Mysore, 570020 India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Sarada
keywords: Eicosapentaenoic acid; Fatty acids; Optimization; Porphyridium; Response surface methodology (RSM)