title: Proteomic Analysis of Human Brown Adipose Tissue Reveals Utilization of Coupled and Uncoupled Energy Expenditure Pathways.
abstract: Human brown adipose tissue (BAT) has become an attractive target to combat the current epidemical spread of obesity and its associated co-morbidities. Currently, information on its functional role is primarily derived from rodent studies. Here, we present the first comparative proteotype analysis of primary human brown adipose tissue versus adjacent white adipose tissue, which reveals significant quantitative differences in protein abundances and in turn differential functional capabilities. The majority of the 318 proteins with increased abundance in BAT are associated with mitochondrial metabolism and confirm the increased oxidative capacity. In addition to uncoupling protein 1 (UCP1), the main functional effector for uncoupled respiration, we also detected the mitochondrial creatine kinases (CKMT1A/B, CKMT2), as effective modulators of ATP synthase coupled respiration, to be exclusively expressed in BAT. The abundant expression and utilization of both energy expenditure pathways in parallel highlights the complex functional involvement of BAT in human physiology.
authors.affiliation: Institute of Food, Nutrition and Health, ETH Zurich, Schwerzenbach, Switzerland.|Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.|Life Science Zurich Graduate School, Molecular Life Sciences Program, Zurich, Switzerland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Müller
authors.affiliation: Institute of Food, Nutrition and Health, ETH Zurich, Schwerzenbach, Switzerland.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Balaz
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery, Faculty of Medicine and University Hospital, Comenius University, Bratislava, Slovakia.
authors.forename: Patrik
authors.initials: P
authors.lastname: Stefanicka
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery, Faculty of Medicine and University Hospital, Comenius University, Bratislava, Slovakia.|Institute of Experimental Endocrinology, Biomedical Research Center at the Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: Lukas
authors.initials: L
authors.lastname: Varga
authors.affiliation: UMR 7277, Centre National de la Recherche Scientifique, U1091, Institut National de la Santé et de la Recherche Médicale, Institute of Biology Valrose, University Nice Sophia Antipolis, Nice, France.
authors.forename: Ez-Zoubir
authors.initials: EZ
authors.lastname: Amri
authors.affiliation: Institute of Experimental Endocrinology, Biomedical Research Center at the Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: Jozef
authors.initials: J
authors.lastname: Ukropec
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Bernd
authors.initials: B
authors.lastname: Wollscheid
authors.affiliation: Institute of Food, Nutrition and Health, ETH Zurich, Schwerzenbach, Switzerland.
authors.forename: Christian
authors.initials: C
authors.lastname: Wolfrum
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D002460:Cell Line; D003402:Creatine Kinase / Q000378:metabolism; D052281:Creatine Kinase, Mitochondrial Form; D004734:Energy Metabolism / Q000502:physiology*; D020869:Gene Expression Profiling; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D020543:Proteome / Q000032:analysis; D000071256:Uncoupling Protein 1 / Q000378:metabolism
chemical_list: D015415:Biomarkers; D020543:Proteome; C000604371:UCP1 protein, human; D000071256:Uncoupling Protein 1; C519581:CKMT1A protein, human; C521170:CKMT2 protein, human; D003402:Creatine Kinase; D052281:Creatine Kinase, Mitochondrial Form