title: Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery.
abstract: In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.
authors.affiliation: a Department of General Surgery , The Second Hospital of Jilin University , Changchun , Jilin , China.
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.affiliation: b Fuda Cancer Hospital , Jinan University School of Medicine, Guangzhou Fuda Cancer Institute , Guangzhou , Guangdong , China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: b Fuda Cancer Hospital , Jinan University School of Medicine, Guangzhou Fuda Cancer Institute , Guangzhou , Guangdong , China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Liang
authors.affiliation: b Fuda Cancer Hospital , Jinan University School of Medicine, Guangzhou Fuda Cancer Institute , Guangzhou , Guangdong , China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Zeng
authors.affiliation: b Fuda Cancer Hospital , Jinan University School of Medicine, Guangzhou Fuda Cancer Institute , Guangzhou , Guangdong , China.
authors.forename: Guifeng
authors.initials: G
authors.lastname: Liu
authors.affiliation: b Fuda Cancer Hospital , Jinan University School of Medicine, Guangzhou Fuda Cancer Institute , Guangzhou , Guangdong , China.
authors.forename: Feng
authors.initials: F
authors.lastname: Mu
authors.affiliation: b Fuda Cancer Hospital , Jinan University School of Medicine, Guangzhou Fuda Cancer Institute , Guangzhou , Guangdong , China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Li
authors.affiliation: b Fuda Cancer Hospital , Jinan University School of Medicine, Guangzhou Fuda Cancer Institute , Guangzhou , Guangdong , China.
authors.forename: Jibing
authors.initials: J
authors.lastname: Chen
authors.affiliation: a Department of General Surgery , The Second Hospital of Jilin University , Changchun , Jilin , China.
authors.forename: Tongjun
authors.initials: T
authors.lastname: Liu
authors.affiliation: b Fuda Cancer Hospital , Jinan University School of Medicine, Guangzhou Fuda Cancer Institute , Guangzhou , Guangdong , China.
authors.forename: Lizhi
authors.initials: L
authors.lastname: Niu
mesh_terms: D015179:Colorectal Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology / Q000601:surgery*; D003452:Cryosurgery / Q000379:methods*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary* / Q000601:surgery*; D008297:Male; D009362:Neoplasm Metastasis; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D011379:Prognosis
keywords: Circulating tumor cells; colorectal cancer; cryosurgery; flow cytometry; liver metastasis; reverse transcriptase polymerase chain reaction