title: A composite microbial agent containing bacterial and fungal species: Optimization of the preparation process, analysis of characteristics, and use in the purification for volatile organic compounds.
abstract: Proper preservation of microbial activity over long periods poses a considerable challenge for pollutant biopurification. A composite microbial agent, mainly composed of bacteria and fungi isolated by the current research team, was constructed in this study and its performance in the removal of mixed waste gases (containing α-pinene, n-butyl acetate and o-xylene) was investigated. According to the removal efficiency in the first 24h and the response to starvation, the optimal ratio of selected carriers (activated carbon, wheat bran and sawdust) was found to be 1:2:1. In some cases of storages, the removal capability of the microbial agent was more than twice that of the suspension. Microbial analysis showed that the inoculated bacterial and fungal strains dominated the agent preparation and utilization. These results indicated that the agent has potential for use in biopurification of mixed waste gas, favoring the reduction of environmental passives and longer retention of microbial activity.
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Zhuowei
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Lu
authors.affiliation: Chemical Engineering Laboratory and Center for Advance Scientific Research (CICA), Faculty of Sciences, University of La Coruña, Spain.
authors.forename: Christian
authors.initials: C
authors.lastname: Kennes
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Jiexu
authors.initials: J
authors.lastname: Ye
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Yu
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Chen
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou, China. Electronic address: c82z09w12@163.com.
authors.forename: Jianmeng
authors.initials: J
authors.lastname: Chen
mesh_terms: D059827:Agricultural Inoculants / Q000254:growth & development / Q000378:metabolism*; D000393:Air Pollutants / Q000737:chemistry / Q000378:metabolism*; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D000081008:Bicyclic Monoterpenes; D019149:Bioreactors; D005658:Fungi / Q000254:growth & development / Q000378:metabolism*; D005740:Gases / Q000737:chemistry / Q000378:metabolism; D039821:Monoterpenes / Q000737:chemistry / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000737:chemistry / Q000378:metabolism*; D014934:Wood / Q000382:microbiology; D014992:Xylenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000393:Air Pollutants; D000081008:Bicyclic Monoterpenes; D005740:Gases; D039821:Monoterpenes; D055549:Volatile Organic Compounds; D014992:Xylenes; C005451:alpha-pinene; C026114:2-xylene
keywords: Fungi/bacteria; Microbial analysis; Mixed VOCs; Removal performance; Solid agents