title: Tissue-Engineered Vascular Rings from Human iPSC-Derived Smooth Muscle Cells.
abstract: There is an urgent need for an efficient approach to obtain a large-scale and renewable source of functional human vascular smooth muscle cells (VSMCs) to establish robust, patient-specific tissue model systems for studying the pathogenesis of vascular disease, and for developing novel therapeutic interventions. Here, we have derived a large quantity of highly enriched functional VSMCs from human induced pluripotent stem cells (hiPSC-VSMCs). Furthermore, we have engineered 3D tissue rings from hiPSC-VSMCs using a facile one-step cellular self-assembly approach. The tissue rings are mechanically robust and can be used for vascular tissue engineering and disease modeling of supravalvular aortic stenosis syndrome. Our method may serve as a model system, extendable to study other vascular proliferative diseases for drug screening. Thus, this report describes an exciting platform technology with broad utility for manufacturing cell-based tissues and materials for various biomedical applications.
authors.affiliation: Section of Cardiovascular Medicine, Department of Internal Medicine, Yale Cardiovascular Research Center, Yale School of Medicine, New Haven, CT 06511, USA; Yale Stem Cell Center, Yale University, New Haven, CT 06510, USA; Department of Surgery (Plastic), Yale University, New Haven, CT 06520, USA.
authors.forename: Biraja C
authors.initials: BC
authors.lastname: Dash
authors.affiliation: Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
authors.forename: Karen
authors.initials: K
authors.lastname: Levi
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, CT 06510, USA.
authors.forename: Jonas
authors.initials: J
authors.lastname: Schwan
authors.affiliation: Section of Cardiovascular Medicine, Department of Internal Medicine, Yale Cardiovascular Research Center, Yale School of Medicine, New Haven, CT 06511, USA; Yale Stem Cell Center, Yale University, New Haven, CT 06510, USA.
authors.forename: Jiesi
authors.initials: J
authors.lastname: Luo
authors.affiliation: Section of Cardiovascular Medicine, Department of Internal Medicine, Yale Cardiovascular Research Center, Yale School of Medicine, New Haven, CT 06511, USA; Yale Stem Cell Center, Yale University, New Haven, CT 06510, USA.
authors.forename: Oscar
authors.initials: O
authors.lastname: Bartulos
authors.affiliation: Section of Cardiovascular Medicine, Department of Internal Medicine, Yale Cardiovascular Research Center, Yale School of Medicine, New Haven, CT 06511, USA; Yale Stem Cell Center, Yale University, New Haven, CT 06510, USA; Department of Orthopedics, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410011, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wu
authors.affiliation: Yale Stem Cell Center, Yale University, New Haven, CT 06510, USA.
authors.forename: Caihong
authors.initials: C
authors.lastname: Qiu
authors.affiliation: Section of Cardiovascular Medicine, Department of Internal Medicine, Yale Cardiovascular Research Center, Yale School of Medicine, New Haven, CT 06511, USA; Yale Stem Cell Center, Yale University, New Haven, CT 06510, USA.
authors.forename: Ting
authors.initials: T
authors.lastname: Yi
authors.affiliation: Section of Cardiovascular Medicine, Department of Internal Medicine, Yale Cardiovascular Research Center, Yale School of Medicine, New Haven, CT 06511, USA; Yale Stem Cell Center, Yale University, New Haven, CT 06510, USA.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, CT 06510, USA.
authors.forename: Stuart
authors.initials: S
authors.lastname: Campbell
authors.affiliation: Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
authors.forename: Marsha W
authors.initials: MW
authors.lastname: Rolle
authors.affiliation: Section of Cardiovascular Medicine, Department of Internal Medicine, Yale Cardiovascular Research Center, Yale School of Medicine, New Haven, CT 06511, USA; Yale Stem Cell Center, Yale University, New Haven, CT 06510, USA; Vascular Biology and Therapeutics Program, Yale School of Medicine, New Haven, CT 06510, USA; Department of Pathology, Yale University, New Haven, CT 06510, USA. Electronic address: yibing.qyang@yale.edu.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Qyang
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000254:growth & development* / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D010641:Phenotype; D023822:Tissue Engineering