title: Early-age-dependent selective decrease of differentiation potential of hair-follicle-associated pluripotent (HAP) stem cells to beating cardiac-muscle cells.
abstract: We have previously discovered nestin-expressing hair-follicle-associated pluripotent (HAP) stem cells and have shown that they can differentiate to neurons, glia, and many other cell types. HAP stem cells can be used for nerve and spinal cord repair. We have recently shown the HAP stem cells can differentiate to beating heart-muscle cells and tissue sheets of beating heart-muscle cells. In the present study, we determined the efficiency of HAP stem cells from mouse vibrissa hair follicles of various ages to differentiate to beating heart-muscle cells. We observed that the whiskers located near the ear were more efficient to differentiate to cardiac-muscle cells compared to whiskers located near the nose. Differentiation to cardiac-muscle cells from HAP stem cells in cultured whiskers in 4-week-old mice was significantly greater than in 10-, 20-, and 40-week-old mice. There was a strong decrease in differentiation potential of HAP stem cells to cardiac-muscle cells by 10 weeks of age. In contrast, the differentiation potential of HAP stem cells to other cell types did not decrease with age. The possibility of rejuvenation of HAP stem cells to differentiate at high efficiency to cardiac-muscle cells is discussed.
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine, Minami Ward , Sagamihara , Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Yamazaki
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine, Minami Ward , Sagamihara , Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hamada
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine, Minami Ward , Sagamihara , Japan.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Arakawa
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine, Minami Ward , Sagamihara , Japan.
authors.forename: Masateru
authors.initials: M
authors.lastname: Yashiro
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine, Minami Ward , Sagamihara , Japan.
authors.forename: Sumiyuki
authors.initials: S
authors.lastname: Mii
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine, Minami Ward , Sagamihara , Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Aki
authors.affiliation: b Department of Physiology , Kitasato University School of Medicine, Minami Ward , Sagamihara , Japan.|c Department of Cellular & Molecular Physiology , Kitasato University Graduate School of Medical Sciences , Minami Ward, Sagamihara , Japan.
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Kawahara
authors.affiliation: d AntiCancer, Inc. , San Diego , CA , USA.|e Department of Surgery , University of California San Diego , San Diego , CA , USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine, Minami Ward , Sagamihara , Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Amoh
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D018859:Hair Follicle / Q000166:cytology*; D008810:Mice, Inbred C57BL; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014738:Vibrissae / Q000166:cytology
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger
keywords: aging; cardiac-muscle cells; differentiation; hair follicle; nestin; stem cell; whisker