title: Photon Counting System for High-Sensitivity Detection of Bioluminescence at Optical Fiber End.
abstract: The technique of photon counting is widely used for various fields and also applicable to a high-sensitivity detection of luminescence. Thanks to recent development of single photon detectors with avalanche photodiodes (APDs), the photon counting system with an optical fiber has become powerful for a detection of bioluminescence at an optical fiber end, because it allows us to fully use the merits of compactness, simple operation, highly quantum efficiency of the APD detectors. This optical fiber-based system also has a possibility of improving the sensitivity to a local detection of Adenosine triphosphate (ATP) by high-sensitivity detection of the bioluminescence. In this chapter, we are introducing a basic concept of the optical fiber-based system and explaining how to construct and use this system.
authors.affiliation: Graduate school of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, 739-8530, Hiroshima, Japan. iinuma@hiroshima-u.ac.jp.
authors.forename: Masataka
authors.initials: M
authors.lastname: Iinuma
authors.affiliation: Graduate school of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, 739-8530, Hiroshima, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kadoya
authors.affiliation: Graduate school of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, 739-8530, Hiroshima, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Kuroda
mesh_terms: D000255:Adenosine Triphosphate; D004867:Equipment Design; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D055100:Optical Fibers; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods / Q000592:standards; D017785:Photons; D012680:Sensitivity and Specificity
chemical_list: D000255:Adenosine Triphosphate
keywords: Adenosine triphosphate; Avalanche photodiode (APD); Bioluminescence; High sensitivity; Luciferase; Optical fiber; Photon counting; Single photon detector