title: A glucuronoyl esterase from Acremonium alcalophilum cleaves native lignin-carbohydrate ester bonds.
abstract: The Glucuronoyl esterases (GE) have been proposed to target lignin-carbohydrate (LC) ester bonds between lignin moieties and glucuronic acid side groups of xylan, but to date, no direct observations of enzymatic cleavage on native LC ester bonds have been demonstrated. In the present investigation, LCC fractions from spruce and birch were treated with a recombinantly produced GE originating from Acremonium alcalophilum (AaGE1). A combination of size exclusion chromatography and (31) P NMR analyses of phosphitylated LCC samples, before and after AaGE1 treatment provided the first evidence for cleavage of the LC ester linkages existing in wood.
authors.affiliation: Department of Biology and Biological Engineering, Wallenberg Wood Science Center, Chalmers University of Technology, Gothenburg, Sweden.
authors.forename: Jenny
authors.initials: J
authors.lastname: Arnling Bååth
authors.affiliation: Department of Fiber and Polymer Technology, Wallenberg Wood Science Center, Royal Institute of Technology, KTH, Stockholm, Sweden.
authors.forename: Nicola
authors.initials: N
authors.lastname: Giummarella
authors.affiliation: Department of Biology and Biological Engineering, Wallenberg Wood Science Center, Chalmers University of Technology, Gothenburg, Sweden.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Klaubauf
authors.affiliation: Department of Fiber and Polymer Technology, Wallenberg Wood Science Center, Royal Institute of Technology, KTH, Stockholm, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Lawoko
authors.affiliation: Department of Biology and Biological Engineering, Wallenberg Wood Science Center, Chalmers University of Technology, Gothenburg, Sweden.
authors.forename: Lisbeth
authors.initials: L
authors.lastname: Olsson
mesh_terms: D000164:Acremonium / Q000201:enzymology; D029662:Betula / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry* / Q000276:immunology; D004950:Esterases / Q000737:chemistry* / Q000378:metabolism; D004952:Esters / Q000737:chemistry* / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D008031:Lignin / Q000737:chemistry; D028222:Picea / Q000737:chemistry; D014990:Xylans / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D004952:Esters; D014990:Xylans; D020723:Glucuronic Acid; D008031:Lignin; D004950:Esterases
keywords: 31P NMR; carbohydrate esterase; lignin-carbohydrate complexes; size exclusion chromatography; spruce and birch