title: Exogenous PML/RARα Fusion Gene Responds to All-trans Retinoic Acid Results in Differentiation of the Human B Cell Line.
abstract: The interaction of an exogenous PML/RARα fusion gene, associated with acute promyelocytic leukemia, with all-trans retinoic acid (ATRA) was examined in B-lymphoid cell lines. RPMI8866 cells were transfected with PML/RARα cDNA in the expression vector pGD and two stable transformants (RPMI8866Y-4 and RPMI8866Y-17) were established by selection with G418. ATRA inhibited the growth of those stable transformants, as assessed by [(3)H]-thymidine incorporation, but had no effect on the growth of control cells stably transformed with neomycin resistant gene alone. ATRA also increased expression of CD38 and immunoglobulin production in RPMI8866Y-4 cells but not in control cells. When these results are taken together, it can be observed that the exogenous PML/RARα fusion gene responds to ATRA, which results in cell differentiation of the human B cell line.
authors.affiliation: a Third Department of Internal Medicine , Kinki University School of Medicine , Osaka , Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sumimoto
authors.affiliation: a Third Department of Internal Medicine , Kinki University School of Medicine , Osaka , Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Maeda
authors.affiliation: a Third Department of Internal Medicine , Kinki University School of Medicine , Osaka , Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Naiki
authors.affiliation: a Third Department of Internal Medicine , Kinki University School of Medicine , Osaka , Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sono
authors.affiliation: a Third Department of Internal Medicine , Kinki University School of Medicine , Osaka , Japan.
authors.forename: J
authors.initials: J
authors.lastname: Miyatake
authors.affiliation: a Third Department of Internal Medicine , Kinki University School of Medicine , Osaka , Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sakaguchi
authors.affiliation: a Third Department of Internal Medicine , Kinki University School of Medicine , Osaka , Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
authors.affiliation: a Third Department of Internal Medicine , Kinki University School of Medicine , Osaka , Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kanamaru
keywords: ATRA; B-lymphocytes; Exogenous PML/RARα gene; differentiation