title: Platelet-Rich Fibrin Lysate Can Ameliorate Dysfunction of Chronically UVA-Irradiated Human Dermal Fibroblasts.
abstract: To determine whether platelet-rich fibrin lysate (PRF-L) could restore the function of chronically ultraviolet-A (UVA)-irradiated human dermal fibroblasts (HDFs), we isolated and sub-cultured HDFs from six different human foreskins. HDFs were divided into two groups: those that received chronic UVA irradiation (total dosages of 10 J cm⁻²) and those that were not irradiated. We compared the proliferation rates, collagen deposition, and migration rates between the groups and between chronically UVA-irradiated HDFs in control and PRF-L-treated media. Our experiment showed that chronic UVA irradiation significantly decreased (p<0.05) the proliferation rates, migration rates, and collagen deposition of HDFs, compared to controls. Compared to control media, chronically UVA-irradiated HDFs in 50% PRF-L had significantly increased proliferation rates, migration rates, and collagen deposition (p<0.05), and the migration rates and collagen deposition of chronically UVA-irradiated HDFs in 50% PRF-L were equal to those of normal fibroblasts. Based on this experiment, we concluded that PRF-L is a good candidate material for treating UVA-induced photoaging of skin, although the best method for its clinical application remains to be determined.
authors.affiliation: Department of Dermato-Venereology, Faculty of Medicine, Gadjah Mada University, Sardjito Hospital, Yogyakarta, Indonesia. widiokarsono@yahoo.com.
authors.forename: Yohanes Widodo
authors.initials: YW
authors.lastname: Wirohadidjojo
authors.affiliation: Department of Dermato-Venereology, Faculty of Medicine, Gadjah Mada University, Sardjito Hospital, Yogyakarta, Indonesia.
authors.forename: Arief
authors.initials: A
authors.lastname: Budiyanto
authors.affiliation: Department of Dermato-Venereology, Faculty of Medicine, Gadjah Mada University, Sardjito Hospital, Yogyakarta, Indonesia.
authors.forename: Hardyanto
authors.initials: H
authors.lastname: Soebono
mesh_terms: D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism*; D002465:Cell Movement / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005337:Fibrin / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D012867:Skin / Q000166:cytology*; D013997:Time Factors; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D005337:Fibrin; D003094:Collagen
keywords: Collagen; cell migration; cell proliferation; platelet-rich fibrin; ultraviolet-A