title: Pregnancy-Associated Changes of IgG and Serum N-Glycosylation in Camel (Camelus dromedarius).
abstract: The dromedary camel (Camelus dromedarius) is an agriculturally important species of high economic value but of low reproductive efficiency. Serum and IgG N-glycosylation are affected by physiological and pathogenic changes and might therefore be a useful diagnostic tool in camel livestock management. This study presents the first comprehensive annotation of the N-glycome from dromedary camel serum as well as their single-domain and conventional antibodies and its subsequent application for camel pregnancy diagnostics. N-glycans were released by PNGaseF, labeled with 2-aminobenzamide (2-AB), and analyzed by hydrophilic interaction liquid chromatography with fluorescent detection (HILIC-UPLC-FLD), enzymatic sequencing and mass spectrometry (MS). The use of a high-throughput robotic platform for sample preparation allowed the rapid generation of glycomics data from pregnant (n = 8) and nonpregnant (n = 8) camels of the Majaheem and Wadha breed. IgG N-glycans dominate the glycan profile of camel serum and present a mixture of core-fucosylated and noncore-fucosylated N-glycans which can contain N-glycolylneuraminic and N-acetylneuraminic acid. Significant pregnancy-associated but breed-independent increases in galactosylation, core-fucosylation, sialylation, and decreases in serum O-acetylation were observed. The monitoring of IgG and serum N-glycosylation presents an attractive complementary test for camel pregnancy diagnostics and presents an interesting tool for biomarker discovery in camel health and breeding.
authors.affiliation: GlycoScience Group, NIBRT-The National Institute for Bioprocessing Research and Training , Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, Ireland.|Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg , Gothenburg, 40530, Sweden.
authors.forename: Barbara
authors.initials: B
authors.lastname: Adamczyk
authors.affiliation: GlycoScience Group, NIBRT-The National Institute for Bioprocessing Research and Training , Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, Ireland.
authors.forename: Simone
authors.initials: S
authors.lastname: Albrecht
authors.affiliation: GlycoScience Group, NIBRT-The National Institute for Bioprocessing Research and Training , Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, Ireland.
authors.forename: Henning
authors.initials: H
authors.lastname: Stöckmann
authors.forename: Ibrahim M
authors.initials: IM
authors.lastname: Ghoneim
authors.forename: Marzook
authors.initials: M
authors.lastname: Al-Eknah
authors.forename: Khalid A S
authors.initials: KA
authors.lastname: Al-Busadah
authors.affiliation: Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg , Gothenburg, 40530, Sweden.
authors.forename: Niclas G
authors.initials: NG
authors.lastname: Karlsson
authors.affiliation: School of Agriculture and Food Science, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Carrington
authors.affiliation: GlycoScience Group, NIBRT-The National Institute for Bioprocessing Research and Training , Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, Ireland.
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002162:Camelus; D002853:Chromatography, Liquid; D003933:Diagnosis; D005260:Female; D054794:Glycomics / Q000379:methods*; D006031:Glycosylation; D007074:Immunoglobulin G / Q000378:metabolism*; D013058:Mass Spectrometry; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism; D011247:Pregnancy; D044967:Serum / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D007074:Immunoglobulin G; D011134:Polysaccharides
keywords: HILIC−UPLC-FLD−MS; IgG; N-glycome; dromedary camel; pregnancy; serum