title: Dominant plasmids carrying extended-spectrum β-lactamases blaCTX-Mgenes in genetically diverse Escherichia coli from slaughterhouse and urban wastewaters.
abstract: Wastewater treatment plants (WWTP) receiving effluents from food-producing animals and humans may contribute to the spread of extended-spectrum β-lactamases (ESBL)-carrying plasmids. This study was designed to investigate extended-spectrum cephalosporin resistant Escherichia coli strains, CTX-M distributions and the genetic lineage of blaCTX-M-carrying plasmids from urban and slaughterhouse wastewaters. The level of extended-spectrum cephalosporin-resistant E. coli in slaughterhouse wastewater entering the WWTP was negligible compared with that of urban wastewater. The blaCTX-M-1gene was predominant in slaughterhouse wastewater whereas diverse blaCTX-Mgenes were encountered in urban wastewater and WWTP outlet. Characterization of the main CTX-M-producing E. coli isolates by antibiotic resistance phenotyping, genotyping and typing of plasmids carrying blaCTX-Mgenes revealed that blaCTX-M-1and blaCTX-M-15genes were harboured by the predominant blaCTX-M-1IncI1/ST3 and blaCTX-M-15F31:A4:B1 plasmids, which were recovered from unrelated E. coli genotypes in both slaughterhouse and urban wastewaters. This study highlighted the spread of predominant blaCTX-M-1and blaCTX-M-15plasmid lineages in diverse E. coli genotypes from humans and food-producing animals, their mixing in WWTP and final release into the aquatic environment. This could have a serious negative impact on public health and requires further evaluation.
authors.affiliation: Toxalim, Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Dupouy
authors.affiliation: UMR1282 Infectiologie et Santé Publique, INRA, Nouzilly, F-37380, France.|UMR1282 Infectiologie et Santé Publique, Université François Rabelais de Tours, Tours, F-37000, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Doublet
authors.affiliation: Toxalim, Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Arpaillange
authors.affiliation: UMR1282 Infectiologie et Santé Publique, INRA, Nouzilly, F-37380, France.|UMR1282 Infectiologie et Santé Publique, Université François Rabelais de Tours, Tours, F-37000, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Praud
authors.affiliation: IRSD, Université de Toulouse, INSERM, INRA, ENVT, UPS, Toulouse, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Bibbal
authors.affiliation: IRSD, Université de Toulouse, INSERM, INRA, ENVT, UPS, Toulouse, France.
authors.forename: Hubert
authors.initials: H
authors.lastname: Brugère
authors.affiliation: IRSD, Université de Toulouse, INSERM, INRA, ENVT, UPS, Toulouse, France.|CHU de Toulouse, Hôpital Purpan, Toulouse, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Oswald
authors.affiliation: UMR1282 Infectiologie et Santé Publique, INRA, Nouzilly, F-37380, France.|UMR1282 Infectiologie et Santé Publique, Université François Rabelais de Tours, Tours, F-37000, France.
authors.forename: Axel
authors.initials: A
authors.lastname: Cloeckaert
authors.affiliation: Toxalim, Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse, France.
authors.forename: Pierre-Louis
authors.initials: PL
authors.lastname: Toutain
authors.affiliation: Toxalim, Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Bousquet-Mélou