title: Quantification of 4-hydroxy-2-nonenal-protein adducts in the in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation.
abstract: Hydroxyalkenals are lipid oxidation end-products resulting from the oxidation of polyunsaturated fatty acids (PUFA). This study aimed at quantifying the production of 4-hydroxy-2-nonenal-protein adducts (HNE-P) via Michael addition from n-6 PUFA oxidation in the gastric digesta of mini-pigs after the consumption of meat-based meals with different plant antioxidant contents. Using the accuracy profile procedure, we validated an extraction protocol for the quantification of HNE-P by GC-MS/MS in gastric contents. The formation of HNE-P in the gastric compartment was observed for the first time, with concentrations ranging from less than 0.52 to 1.33 nmol HNE-P per 500 mg digesta. Nevertheless, most gastric HNE-P levels were below the limit of quantification of 0.52 nmol HNE-P per 500 mg digesta. In this animal study, the protective effect of plant antioxidant sources on HNE-P formation was not evidenced contrasting with the results using TBARS as markers.
authors.affiliation: INRA, UR370 Animal Products Quality Unit, Clermont-Ferrand/Theix, F-63122 Saint-Genès-Champanelle, France. mylene.delosiere@clermont.inra.fr.
authors.forename: Mylène
authors.initials: M
authors.lastname: Delosière
authors.forename: Véronique
authors.initials: V
authors.lastname: Santé-Lhoutellier
authors.forename: Céline
authors.initials: C
authors.lastname: Chantelauze
authors.forename: Denys
authors.initials: D
authors.lastname: Durand
authors.forename: Agnès
authors.initials: A
authors.lastname: Thomas
authors.forename: Charlotte
authors.initials: C
authors.lastname: Joly
authors.forename: Estelle
authors.initials: E
authors.lastname: Pujos-Guillot
authors.forename: Didier
authors.initials: D
authors.lastname: Rémond
authors.forename: Blandine
authors.initials: B
authors.lastname: Comte
authors.forename: Cécile
authors.initials: C
authors.lastname: Gladine
authors.forename: Alexandre
authors.initials: A
authors.lastname: Guy
authors.forename: Thierry
authors.initials: T
authors.lastname: Durand
authors.forename: Michel
authors.initials: M
authors.lastname: Laurentie
authors.forename: Claire
authors.initials: C
authors.lastname: Dufour
mesh_terms: D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D004032:Diet; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D005766:Gastrointestinal Contents / Q000737:chemistry*; D041981:Gastrointestinal Tract / Q000378:metabolism*; D050356:Lipid Metabolism; D062407:Meals; D008460:Meat; D023421:Models, Animal; D010084:Oxidation-Reduction; D010944:Plants / Q000737:chemistry; D015203:Reproducibility of Results; D013552:Swine; D013556:Swine, Miniature; D053719:Tandem Mass Spectrometry; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000447:Aldehydes; D000975:Antioxidants; D005231:Fatty Acids, Unsaturated; D017392:Thiobarbituric Acid Reactive Substances; C027576:4-hydroxy-2-nonenal