title: Edaravone improves survival and neurological outcomes after CPR in a ventricular fibrillation model of rats.
abstract: OBJECTIVE
  Overproduction of free radicals is a main factor contributing to cerebral injury after cardiac arrest (CA)/cardiopulmonary resuscitation (CPR). We sought to evaluate the impact of edaravone on the survival and neurological outcomes after CA/CPR in rats.
  
  
  METHODS
  Rats were subjected to CA following CPR. For survival study, the rats with restoration of spontaneous circulation (ROSC) were randomly allocated to one of the two groups (edaravone and saline group, n=20/each group) to received Edaravone (3 mg/kg) or normal saline. Another 10 rats without experiencing CA and CPR served as the sham group. Survival was observed for 72 hours and the neurological deficit score (NDS) was calculated at 12, 24, 48, and 72 hours after ROSC. For the neurological biochemical analysis study, rats were subjected to the same experimental procedures. Then, edaravone group (n=24), saline group (n=24) and sham group (n=16) were further divided into 4 subgroups according to the different time intervals (12, 24, 48, and 72 hours following ROSC). Brain tissues were harvested at relative time intervals for evaluation of oxidative stress, TUNEL staining and apoptotic gene expression.
  
  
  RESULTS
  Edaravone improved postresuscitative survival time and neurological deficit, decreased brain malonylaldehyde level, increased superoxide dismutase activities, decreased proapoptotic gene expression of capase-8, capase-3, and Bax, and increased antiapoptotic Bcl-2 expression at 12, 24, 48, and 72 hours after ROSC.
  
  
  CONCLUSIONS
  Edaravone improves survival and neurological outcomes following CPR via antioxidative and antiapoptotic effects in rats.
authors.affiliation: Department of Physiology, School of Pre-Clinical Sciences, Guangxi Medical University, Nanning, Guangxi, 530021, China; Institute of Cardiovascular Diseases, The First Hospital Affiliated to Guangxi Medical University, Nanning, Guangxi, 530027, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Qin
authors.affiliation: Department of Physiology, School of Pre-Clinical Sciences, Guangxi Medical University, Nanning, Guangxi, 530021, China; Cardiovascular Department, The Ming Zu Hospital of Guangxi, Nanning, Guangxi, 530001, China.
authors.forename: Ling-Yan
authors.initials: LY
authors.lastname: Lei
authors.affiliation: Department of Physiology, School of Pre-Clinical Sciences, Guangxi Medical University, Nanning, Guangxi, 530021, China; Institute of Cardiovascular Diseases, The First Hospital Affiliated to Guangxi Medical University, Nanning, Guangxi, 530027, China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Physiology, School of Pre-Clinical Sciences, Guangxi Medical University, Nanning, Guangxi, 530021, China.
authors.forename: Fangying Ruan
authors.initials: FR
authors.lastname: Shi
authors.affiliation: Institute of Cardiovascular Diseases, The First Hospital Affiliated to Guangxi Medical University, Nanning, Guangxi, 530027, China.
authors.forename: Meng-Hua
authors.initials: MH
authors.lastname: Chen
authors.affiliation: Department of Physiology, School of Pre-Clinical Sciences, Guangxi Medical University, Nanning, Guangxi, 530021, China. Electronic address: xielu8282@163.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Xie
mesh_terms: D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D001930:Brain Injuries / Q000209:etiology / Q000517:prevention & control; D016887:Cardiopulmonary Resuscitation / Q000379:methods*; D004195:Disease Models, Animal; D000077553:Edaravone; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D006323:Heart Arrest / Q000188:drug therapy / Q000628:therapy; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014693:Ventricular Fibrillation / Q000188:drug therapy* / Q000628:therapy
chemical_list: D016166:Free Radical Scavengers; D000077553:Edaravone; D000983:Antipyrine