title: Antihypertensive effects of fargesin in vitro and in vivo via attenuating oxidative stress and promoting nitric oxide release.
abstract: Fargesin, a bioactive neolignan isolated from magnolia plants, is widely used in the treatment of managing rhinitis, inflammation, histamine, sinusitis, and headache. To provide more biological information about fargesin, we investigated the effects of fargesin on rat aortic rings and 2-kidney, 1-clip (2K1C) hypertensive rats. In vitro, fargesin caused concentration-dependent vasorelaxation in rat isolated aortic rings induced by KCl and norepinephrine. The effect was weakened by endothelium denudation and nitric oxide (NO) synthesis inhibition. In vivo, the evolution of systolic blood pressure (SBP) was followed by weekly measurements. Angiotensin II (Ang II) and endothelin (ET) levels, NO and nitric oxide synthase (NOS), and plasma and liver oxidative stress markers were determined at the end of the experimental period. After 5 weeks of fargesin treatment, we found that fargesin treatment reduced SBP, cardiac hypertrophy, and Ang II and ET levels of hypertensive rats. Increased NOS activity and NO level were observed in fargesin-treated rats. Normalisation of plasma MDA concentrations and improvement of the antioxidant defence system in plasma and liver accompanied the antihypertensive effect of fargesin. Taken together, these results provided substantial evidences that fargesin has antihypertensive effect in 2K1C hypertensive rats via inhibiting oxidative stress and promoting NO release.
authors.affiliation: College of Pharmacy, Shanxi Medical University, Taiyuan 030001, People's Republic of China.|College of Pharmacy, Shanxi Medical University, Taiyuan 030001, People's Republic of China.
authors.forename: Sha
authors.initials: S
authors.lastname: Sha
authors.affiliation: College of Pharmacy, Shanxi Medical University, Taiyuan 030001, People's Republic of China.|College of Pharmacy, Shanxi Medical University, Taiyuan 030001, People's Republic of China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Xu
authors.affiliation: College of Pharmacy, Shanxi Medical University, Taiyuan 030001, People's Republic of China.|College of Pharmacy, Shanxi Medical University, Taiyuan 030001, People's Republic of China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Shanxi Medical University, Taiyuan 030001, People's Republic of China.|College of Pharmacy, Shanxi Medical University, Taiyuan 030001, People's Republic of China.
authors.forename: Weifang
authors.initials: W
authors.lastname: Zhao
authors.affiliation: College of Pharmacy, Shanxi Medical University, Taiyuan 030001, People's Republic of China.|College of Pharmacy, Shanxi Medical University, Taiyuan 030001, People's Republic of China.
authors.forename: Xiaoni
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism; D052117:Benzodioxoles / Q000494:pharmacology / Q000627:therapeutic use*; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D006973:Hypertension / Q000188:drug therapy* / Q000378:metabolism; D017705:Lignans / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D009924:Organ Culture Techniques; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology
chemical_list: D000959:Antihypertensive Agents; D052117:Benzodioxoles; D017705:Lignans; D009569:Nitric Oxide; C539377:fargesin
keywords: 2K1C hypertensive rats; anneaux aortiques de rat; antioxidant; antioxydant; fargesin; fargésine; rat aortic rings; rats hypertendus 2 reins, 1 clip (2K1C); vasorelaxation