title: Dataset of protein changes induced by cold acclimation in red clover (Trifolium pratense L.) populations recurrently selected for improved freezing tolerance.
abstract: The data provide an overview of proteomic changes in red clover (Trifolium pratense L.) in response to cold acclimation and recurrent selection for superior freezing tolerance. Proteins were extracted from crowns of two red clover cultivars grown under non-acclimated or cold-acclimated conditions, and plants obtained from the initial genetic background (TF0) and from populations obtained after three (TF3) and four cycles (TF4) of recurrent selection for superior freezing tolerance. Proteins were analyzed using a two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled to mass spectroscopy (MS and MS/MS). Differentially regulated proteins were subsequently identified using MALDI TOF/TOF analysis. The data are related to a recently published research article describing proteome composition changes associated with freezing tolerance in red clover, "A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)" (Bertrand et al., 2016 [1]). They are available in the ProteomeXchange Consortium database via the PRIDE partner repository under the dataset identifier PRIDE: PXD003689.
authors.affiliation: Agriculture and Agri-Food Canada, Québec City, Canada.
authors.forename: Marie
authors.initials: M
authors.lastname: Bipfubusa
authors.affiliation: Agriculture and Agri-Food Canada, Québec City, Canada.
authors.forename: Solen
authors.initials: S
authors.lastname: Rocher
authors.affiliation: Agriculture and Agri-Food Canada, Québec City, Canada.
authors.forename: Annick
authors.initials: A
authors.lastname: Bertrand
authors.affiliation: Agriculture and Agri-Food Canada, Québec City, Canada.
authors.forename: Yves
authors.initials: Y
authors.lastname: Castonguay
authors.affiliation: Luxembourg Institute of Science and Technology, Belvaux, Luxembourg.
authors.forename: Jenny
authors.initials: J
authors.lastname: Renaut
keywords: Cold acclimation; Freezing tolerance; Plant abiotic stress; Proteomic analysis; Recurrent selection; Red clover