title: Developmental Validation of a novel 5 dye Y-STR System comprising the 27 YfilerPlus loci.
abstract: In this study, a new STRtyper-27 system, including the same Yfiler Plus loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, Y-GATA H4, DYS449, DYS460, DYS481, DYS518, DYS533, DYS570, DYS576, DYS627 and DYF387S1a/b), was established using a set of 5 fluorescent dye labels. Primers, internal size standard, allelic ladders and matrix standard set were designed and created in-house for this multiplex system. This paper describes the validation studies conducted with the STRtyper-27Y system using a 3130XL genetic analyzer for fragment length detection that included the analysis of the following parameters and aspects: sensitivity, species specificity, inhibition, haplotype concordance, precision, stutter, DNA mixtures, and stability studies with crime scene samples. The studies demonstrated, that the STRtyper-27Y system provided equivalent overall performance comparable to the latest Yfiler Plus kit, but with enhanced compatibility in terms of instrument platforms and software allowing forensic laboratories to conduct its forensic application and evaluate its performance, all in their own 5 dye Y-STR chemistry system /environment without software or instrument upgrades.
authors.affiliation: Department of Radiology, First Affiliated Hospital, Medical College of Shantou University, Shantou 515041, P.R. China.|Key Laboratory of Evidence Law and Forensic Science, Ministry of Education, Beijing 100088, P.R. China.
authors.forename: Rufeng
authors.initials: R
authors.lastname: Bai
authors.affiliation: Institute of Criminal Sciences and Technology, Municipal Public Security Bureau of Xuchang, Xuchang 461000, P.R. China.
authors.forename: Yaju
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Health GeneTech, Ningbo 315000, P.R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Health GeneTech, Ningbo 315000, P.R. China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Jin
authors.affiliation: Key Laboratory of Evidence Law and Forensic Science, Ministry of Education, Beijing 100088, P.R. China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Tian
authors.affiliation: Key Laboratory of Evidence Law and Forensic Science, Ministry of Education, Beijing 100088, P.R. China.|Collaborative Innovation Center of Judicial Civilization, Beijing 100088, P.R. China.
authors.forename: Meisen
authors.initials: M
authors.lastname: Shi
authors.affiliation: Department of Radiology, First Affiliated Hospital, Medical College of Shantou University, Shantou 515041, P.R. China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Ma