title: Expression of miRNA-21 and miRNA-221 in clear cell renal cell carcinoma (ccRCC) and their possible role in the development of ccRCC.
abstract: Clear cell renal cell carcinoma (ccRCC) is the third most common urological cancer after prostate and bladder cancer but has the highest rate of mortality affecting over 40% of patients. microRNAs (miRNAs) are small noncoding RNAs that have become potential biomarkers and molecular targets for cancer treatment. Molecular markers such as miRNAs may have a role in the diagnosis of ccRCC. In this study, we examined the expressions of miRNA-21 and miRNA-221 in renal cancer patients׳ tumor and adjacent paired normal tissues investigating the possible role of these miRNAs in the development of ccRCC.
  
  
  
  Renal tumors (n = 24) and paired normal renal tissue (n = 24) samples, obtained from the Department of Urology, University of Debrecen, were analyzed for miRNA-21 and miRNA-221 expressions with quantitative real-time polymerase chain reaction.
  
  
  
  miRNA-21 and miRNA-221 expressions were significantly up-regulated in tumor specimens compared to normal tissue (P<0.05). miRNA-21 and miRNA-221 showed coexpression pattern in 19 (79.2%) cases of tumor samples and 8 (33.3%) cases of paired normal renal tissues. Increased miRNA pattern showed a positive correlation with pathological status of the patients.
  
  
  
  Expression of oncogenic miRNA-21 and miRNA-221 in human ccRCC tumor tissue samples compared to adjacent nontumorous tissues might suggest that these miRNAs are involved in the development of ccRCC.
authors.affiliation: Department of Biopharmacy, Faculty of Pharmacy, University of Debrecen, Debrecen, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Szabó
authors.affiliation: Department of Urology, University of Debrecen, Debrecen, Hungary.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Szegedi
authors.affiliation: Institute of Public Health, Faculty of Medicine, University of Pécs, Pécs, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Gombos
authors.affiliation: Department of Pharmaceutical Sciences, Texas A & M School of Pharmacy, Kingsville, TX.
authors.forename: Choudhury
authors.initials: C
authors.lastname: Mahua
authors.affiliation: Department of Urology, University of Debrecen, Debrecen, Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Flaskó
authors.affiliation: Department of Biopharmacy, Faculty of Pharmacy, University of Debrecen, Debrecen, Hungary.
authors.forename: Kristóf
authors.initials: K
authors.lastname: Harda
authors.affiliation: Department of Biopharmacy, Faculty of Pharmacy, University of Debrecen, Debrecen, Hungary. Electronic address: halmos.gabor@pharm.unideb.hu.
authors.forename: Gábor
authors.initials: G
authors.lastname: Halmos
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D002292:Carcinoma, Renal Cell / Q000209:etiology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007668:Kidney / Q000378:metabolism; D007680:Kidney Neoplasms / Q000209:etiology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D008875:Middle Aged; D060787:Neoplasm Grading; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012334:RNA, Neoplasm; C524940:MIRN21 microRNA, human; C525709:MIR221, human
keywords: clear cell renal cell carcinoma (ccRCC); miRNA-21; miRNA-221