title: Oxidized Low-Density Lipoprotein-β2-Glycoprotein I Complex But Not Free Oxidized LDL Is Associated With the Presence and Severity of Coronary Artery Disease.
abstract: Oxidized low-density lipoprotein (oxLDL) and β2-glycoprotein I (β2GPI) have been identified in human atherosclerotic lesions and when complexed have been implicated as a pro-atherothrombotic antigen. We examined the association of free oxLDL and oxLDL-β2GPI complex in patients with coronary artery disease who underwent elective cardiac catheterization. Serum was collected from patients with suspected coronary artery disease immediately before elective cardiac catheterization who were either treated (n = 385) or not treated (n = 150) with statins and from healthy volunteers (n = 134). OxLDL and oxLDL-β2GPI complex levels were determined by enzyme-linked immunosorbent assay. Disease severity was defined angiographically as none-minimal (<20%), moderate (20% to 75%), and severe (>75%) luminal diameter obstruction of any major coronary vessel. Both oxLDL and oxLDL-β2GPI complex were lower in patients on statins (p <0.001). In statin-naive patients, oxLDL-β2GPI complex, but not free oxLDL, was associated with severe coronary artery disease (p = 0.036). However, no association was observed in patients on statins. LDL4 and triglycerides increased with oxLDL-β2GPI complex quartiles (p = 0.001). OxLDL-β2GPI complex (>0.32 U/ml) was predictive of severe atherosclerosis by receiver-operating characteristic curve analysis in statin-naive patients (area under the curve 0.66, p = 0.002). In conclusion, oxLDL-β2GPI appears more predictive of coronary artery disease severity than oxLDL alone in statin-naive patients.
authors.affiliation: Inova Center for Thrombosis Research and Drug Development, Inova Heart and Vascular Institute, Fairfax, Virginia.
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Bliden
authors.affiliation: Division of Medicine, Johns Hopkins University/Sinai Hospital of Baltimore, Baltimore, Maryland.
authors.forename: Rahul
authors.initials: R
authors.lastname: Chaudhary
authors.affiliation: Division of Medicine, Corgenix Inc., Broomfield, Colorado.
authors.forename: Luis R
authors.initials: LR
authors.lastname: Lopez
authors.affiliation: Division of Medicine, Johns Hopkins University/Sinai Hospital of Baltimore, Baltimore, Maryland.
authors.forename: Rongras
authors.initials: R
authors.lastname: Damrongwatanasuk
authors.affiliation: Department of Chemistry, Indiana University South Bend, South Bend, Indiana.
authors.forename: Kirk
authors.initials: K
authors.lastname: Guyer
authors.affiliation: Sinai Center of Thrombosis Research, Baltimore, Maryland.
authors.forename: Martin G
authors.initials: MG
authors.lastname: Gesheff
authors.affiliation: Sinai Center of Thrombosis Research, Baltimore, Maryland.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Franzese
authors.affiliation: Sinai Center of Thrombosis Research, Baltimore, Maryland.
authors.forename: Himabindu
authors.initials: H
authors.lastname: Kaza
authors.affiliation: Inova Center for Thrombosis Research and Drug Development, Inova Heart and Vascular Institute, Fairfax, Virginia.
authors.forename: Udaya S
authors.initials: US
authors.lastname: Tantry
authors.affiliation: Inova Center for Thrombosis Research and Drug Development, Inova Heart and Vascular Institute, Fairfax, Virginia. Electronic address: utantry@lifebridgehealth.org.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Gurbel
mesh_terms: D000368:Aged; D000925:Anticoagulants / Q000097:blood; D050197:Atherosclerosis / Q000097:blood; D015415:Biomarkers / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood* / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D053482:beta 2-Glycoprotein I / Q000097:blood
chemical_list: D000925:Anticoagulants; D015415:Biomarkers; D008077:Lipoproteins, LDL; D053482:beta 2-Glycoprotein I; C099252:oxidized low density lipoprotein