title: CRISPR Screens to Discover Functional Noncoding Elements.
abstract: A major challenge in genomics is to identify functional elements in the noncoding genome. Recently, pooled clustered regularly interspersed palindromic repeat (CRISPR) mutagenesis screens of noncoding regions have emerged as a novel method for finding elements that impact gene expression and phenotype/disease-relevant biological processes. Here we review and compare different approaches for high-throughput dissection of noncoding elements.
authors.affiliation: Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Jason B
authors.initials: JB
authors.lastname: Wright
authors.affiliation: New York Genome Center, New York, NY 10013, USA; Center for Genomics and Systems Biology, Department of Biology, New York University, NY 10003, USA. Electronic address: nsanjana@nygenome.org.
authors.forename: Neville E
authors.initials: NE
authors.lastname: Sanjana
mesh_terms: D064113:CRISPR-Cas Systems / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D015894:Genome, Human; D023281:Genomics; D006801:Humans; D022661:RNA, Untranslated / Q000235:genetics*
chemical_list: D022661:RNA, Untranslated
keywords: CRISPR; Cas9; enhancer; functional genomics; gene regulation; noncoding