title: Evaluating genotoxic risks in Brazilian public health agents occupationally exposed to pesticides: a multi-biomarker approach.
abstract: This is the first study demonstrating genotoxic effects and whole transcriptome analysis on community health agents (CHAs) occupationally exposed to pesticides in Central Brazil. For the transcriptome analysis, we found some genes related to Alzheimer's disease (LRP1), an insulin-like growth factor receptor (IGF2R), immunity genes (IGL family and IGJ), two genes related to inflammatory reaction (CXCL5 and CCL3), one gene related to maintenance of cellular morphology (NHS), one gene considered to be a strong apoptosis inductor (LGALS14), and several transcripts of the neuroblastoma breakpoint family (NBPF). Related to comet assay, we demonstrated a significant increase in DNA damage, measured by the olive tail moment (OTM), in the exposed group compared to the control group. Moreover, we also observed a statistically significant difference in OTM values depending on GSTM1 genotypes. Therefore, Brazilian epidemiological surveillance, an organization responsible for the assessment and management of health risks associated to pesticide exposure to CHA, needs to be more proactive and considers the implications of pesticide exposure for CHA procedures and processes.
authors.affiliation: Programa de Pós-Graduação em Genética e Biologia Molecular, Laboratório de Mutagênese e Radiobiologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Fernanda Craveiro
authors.initials: FC
authors.lastname: Franco
authors.affiliation: Programa de Pós-Graduação em Genética e Biologia Molecular, Laboratório de Mutagênese e Radiobiologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Alessandro Arruda
authors.initials: AA
authors.lastname: Alves
authors.affiliation: Núcleo de Pesquisas Replicon, Departamento de Biologia, Pontifícia Universidade Católica de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Fernanda Ribeiro
authors.initials: FR
authors.lastname: Godoy
authors.affiliation: Programa de Pós-Graduação em Genética e Biologia Molecular, Laboratório de Mutagênese e Radiobiologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Juliana Boaventura
authors.initials: JB
authors.lastname: Avelar
authors.affiliation: Núcleo de Pesquisas Replicon, Departamento de Biologia, Pontifícia Universidade Católica de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Douglas Dantas
authors.initials: DD
authors.lastname: Rodrigues
authors.affiliation: Programa de Pós-Graduação em Genética e Biologia Molecular, Laboratório de Mutagênese e Radiobiologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Thays Millena Alves
authors.initials: TM
authors.lastname: Pedroso
authors.affiliation: Núcleo de Pesquisas Replicon, Departamento de Biologia, Pontifícia Universidade Católica de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Aparecido Divino
authors.initials: AD
authors.lastname: da Cruz
authors.affiliation: Campus II, Itatiaia, Departamento de Genética, Instituto de Ciências Biológicas 1, Universidade Federal de Goiás, Goiânia, Goiás, Cep 74001-970, Brazil.
authors.forename: Fausto
authors.initials: F
authors.lastname: Nomura
authors.affiliation: Programa de Pós-Graduação em Genética e Biologia Molecular, Laboratório de Mutagênese e Radiobiologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. silvadanielamelo@gmail.com.|Campus II, Itatiaia, Departamento de Genética, Instituto de Ciências Biológicas 1, Universidade Federal de Goiás, Goiânia, Goiás, Cep 74001-970, Brazil. silvadanielamelo@gmail.com.|Programa de Pós-Graduação em Ciências Ambientais, Laboratório de Mutagênese e Radiobiologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. silvadanielamelo@gmail.com.|Programa de Pós-Graduação em Biodiversidade Animal, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. silvadanielamelo@gmail.com.
authors.forename: Daniela
authors.initials: D
authors.lastname: de Melo E Silva
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001938:Brazil; D020552:Comet Assay; D004249:DNA Damage; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D016273:Occupational Exposure / Q000032:analysis* / Q000706:statistics & numerical data; D010575:Pesticides / Q000032:analysis / Q000378:metabolism*; D011634:Public Health; D012306:Risk
chemical_list: D015415:Biomarkers; D005982:Glutathione Transferase; D010575:Pesticides
keywords: Comet assay; DNA damage; Differential expression panel; GST genotypes; Occupational exposure; Pesticide genotoxicity