title: A new substance to relax polychaete worms (Annelida) prior to morphological study.
abstract: A variety of chemical substances have been used to relax and/or immobilize polychaete worms, and other invertebrates, prior to specimen preparation for morphological examination. To solve difficulties encountered during the study of nereidid polychaetes (Annelida: Phyllodocida), an experiment was designed and carried out to investigate a new relaxing agent to immobilize nereidid specimens and stimulate pharynx eversion. The new substance, Dentol® (Khoraman laboratory, Iran), a dental anesthetic and antiseptic medicine containing 10% Carvacrol as the effective ingredient, was used for the first time and compared with other substances that have been used traditionally in polychaete studies. Crosstab analysis showed significant differences between different treatment groups, with Dentol® providing much better results for all considered criteria.
authors.affiliation: Dept. of Biology, Faculty of Science, Razi University, Baghe- e- Abrisham 67149 Kermanshah, Iran.
authors.forename: Alieh
authors.initials: A
authors.lastname: Bonyadi-Naeini
authors.affiliation: Faculty of Biology, College of Science, University of Tehran, Tehran, Iran, P.O.Box 14155-6455.
authors.forename: Hassan
authors.initials: H
authors.lastname: Rahimian
authors.affiliation: Museum and Art Gallery of the Northern Territory, P.O. Box 4646, Darwin, Northern Territory 0801, Australia.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Glasby
keywords: Carvacrol; Polychaeta; narcotize; specimen preparation