title: Irisin reverses platelet derived growth factor-BB-induced vascular smooth muscle cells phenotype modulation through STAT3 signaling pathway.
abstract: Vascular smooth muscle cells (VSMCs) phenotype modulation toward a synthetic phenotype is the main cause of cardiovascular disease. As a newly discovered myokine, Irisin is thought to be a promising candidate for the treatment of metabolic disturbances, as well as cardiovascular disease. However, no evidence has been shown for the direct effect of Irisin on VSMCs phenotype modulation and its underling mechanisms. The aim of this study was to explore the effect of Irisin on VSMCs phenotype modulation and the mechanisms involved. In the present study, it was found that Irisin restored the PDGF-BB-induced VSMCs phenotype modulation which exhibited down-regulation of smooth muscle cells (SMC) expression and up-regulation of matrix synthesis related marker expression, as well as proliferative phenotype. Moreover, our research demonstrated that Irisin further activated STAT3 signaling pathways. Finally, by applying an STAT3 inhibitor, WP1066, we revealed the roles of STAT3 in the PDGF-BB-induced VSMCs phenotype modulation when they were treated with Irisin. Taken together, these results demonstrated that Irisin may play a crucial role in regulating VSMCs phenotype modulation via the STAT3 signaling pathway.
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan, 250100, China; Center for Gene and Immunotherapy, The Second Hospital of Shandong University, Jinan, 250012, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Song
authors.affiliation: Genetic Disease Diagnosis Center, Zibo Maternal and Child Health Hospital, Affiliated to Shandong Academy of Medical Science, Zibo, Shandong, 255000, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
authors.affiliation: Medical Research & Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Lv
authors.affiliation: Center for Gene and Immunotherapy, The Second Hospital of Shandong University, Jinan, 250012, China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center for Gene and Immunotherapy, The Second Hospital of Shandong University, Jinan, 250012, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan, 250100, China; Medical Research & Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: Huanjie
authors.initials: H
authors.lastname: Li
authors.affiliation: Center for Gene and Immunotherapy, The Second Hospital of Shandong University, Jinan, 250012, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shao
authors.affiliation: Center for Gene and Immunotherapy, The Second Hospital of Shandong University, Jinan, 250012, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Mu
authors.affiliation: Center for Gene and Immunotherapy, The Second Hospital of Shandong University, Jinan, 250012, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Center for Gene and Immunotherapy, The Second Hospital of Shandong University, Jinan, 250012, China. Electronic address: tangdq@sdu.edu.cn.
authors.forename: Dongqi
authors.initials: D
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan, 250100, China. Electronic address: fangxu@sdu.edu.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Fang
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000077214:Becaplermin; D015153:Blotting, Western; D002478:Cells, Cultured; D005353:Fibronectins / Q000494:pharmacology*; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D010641:Phenotype; D010766:Phosphorylation / Q000187:drug effects; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000199:Actins; C577593:FNDC5 protein, human; D005353:Fibronectins; D008840:Microfilament Proteins; D009124:Muscle Proteins; D020574:Proto-Oncogene Proteins c-sis; D050796:STAT3 Transcription Factor; C051615:transgelin; D000077214:Becaplermin
keywords: Irisin; Phenotype modulation; STAT3; Vascular smooth muscle cells