title: NF90 is a novel influenza A virus NS1-interacting protein that antagonizes the inhibitory role of NS1 on PKR phosphorylation.
abstract: NF90 is a novel host antiviral factor that regulates PKR activation and stress granule formation in influenza A virus (IAV)-infected cells, but the precise mechanisms by which it operates remain unclear. We identified NF90 as a novel interacting protein of IAV nonstructural protein 1 (NS1). The interaction was dependent on the RNA-binding properties of NS1. NS1 associated with NF90 and PKR simultaneously; however, the interaction between NF90 and PKR was restricted by NS1. Knockdown of NF90 promoted inhibition of PKR phosphorylation induced by NS1, while coexpression of NF90 impeded reduction of PKR phosphorylation and stress granule formation triggered by NS1. In summary, NF90 exerts its antiviral activity by antagonizing the inhibitory role of NS1 on PKR phosphorylation.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Institute of Animal Quarantine, Chaoyang District, Beijing, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Chinese Academy of Inspection and Quarantine, Institute of Animal Quarantine, Chaoyang District, Beijing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.affiliation: National Institute for Viral Disease Control and Prevention, Collaboration Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China.
authors.forename: WenFei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Chinese Academy of Inspection and Quarantine, Institute of Animal Quarantine, Chaoyang District, Beijing, China.
authors.forename: HuiYu
authors.initials: H
authors.lastname: Wang
authors.affiliation: Chinese Academy of Inspection and Quarantine, Institute of Animal Quarantine, Chaoyang District, Beijing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Mei
authors.affiliation: Chinese Academy of Inspection and Quarantine, Institute of Animal Quarantine, Chaoyang District, Beijing, China.
authors.forename: ShaoQiang
authors.initials: S
authors.lastname: Wu
authors.affiliation: Chinese Academy of Inspection and Quarantine, Institute of Animal Quarantine, Chaoyang District, Beijing, China.
authors.forename: XiangMei
authors.initials: X
authors.lastname: Lin
authors.affiliation: Chinese Academy of Inspection and Quarantine, Institute of Animal Quarantine, Chaoyang District, Beijing, China.
authors.forename: XueQing
authors.initials: X
authors.lastname: Han
mesh_terms: D057809:HEK293 Cells / Q000821:virology; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D007251:Influenza, Human / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D050779:Nuclear Factor 90 Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000235:genetics; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000378:metabolism*; D014779:Virus Replication / Q000235:genetics; D019892:eIF-2 Kinase / Q000737:chemistry / Q000378:metabolism*
chemical_list: C085820:INS1 protein, influenza virus; D050779:Nuclear Factor 90 Proteins; D016601:RNA-Binding Proteins; D017361:Viral Nonstructural Proteins; C000602769:EIF2AK2 protein, human; D019892:eIF-2 Kinase
keywords: NF90; influenza A virus; nonstructural protein 1; protein kinase R phosphorylation; stress granules