title: Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India.
abstract: This study is to probe the pattern of antibiotic resistance against aminoglycosides and its mechanism in E. coli obtained from patients from Chennai, India. Isolation and identification of pathogens were done on MacConkey agar. Antimicrobial sensitivity testing was done by disc diffusion test. The identification of genes encoding aminoglycoside modifying enzymes was done by Polymerase Chain Reaction (PCR). Out of 98 isolates, 71 (72.45%) isolates were identified as E. coli and the remaining 27 (27.55%) as other bacteria. Disc diffusion method results showed a resistance level of 72.15% for streptomycin, 73.4% for gentamicin, 63.26% for neomycin, 57.14% for tobramycin, 47.9% for netilmicin, and 8.16% for amikacin in E. coli. PCR screening showed the presence of four genes, namely, rrs, aacC2, aacA-aphD, and aphA3, in their plasmid DNA. The results point towards the novel mechanism of drug resistance in E. coli from UTI patients in India as they confirm the presence of genes encoding enzymes that cause resistance to aminoglycoside drugs. This could be an alarm for drug prescription to UTI patients.
authors.affiliation: Department of Biotechnology, Government Degree College Baramulla, Kashmir 193101, India.
authors.forename: Abdul Rouf
authors.identifier: 0000-0002-2897-4419
authors.initials: AR
authors.lastname: Mir
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Assam, India.
authors.forename: Yasir
authors.initials: Y
authors.lastname: Bashir
authors.affiliation: Department of Biotechnology, Government Degree College Baramulla, Kashmir 193101, India.
authors.forename: Firdous Ahmad
authors.initials: FA
authors.lastname: Dar
authors.affiliation: Department of Biotechnology, The New College, Chennai, India.
authors.forename: M
authors.initials: M
authors.lastname: Sekhar
mesh_terms: D000617:Aminoglycosides / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D007194:India; D008826:Microbial Sensitivity Tests; D014552:Urinary Tract Infections / Q000382:microbiology*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents