title: Detection and characterization of Aichi virus 1 in pediatric patients with diarrhea in Thailand.
abstract: Kobuvirus is a newly discovered virus that belongs to the Kobuvirus genus in Picornaviridae family, which comprised of three species including Aichivirus A, Aichivirus B, and Aichivirus C. The kobuvirus isolated from human has been classified as Aichi virus 1 and belongs to Aichivirus A species. The present study aimed to assess the epidemiology and to perform molecular characterization of Aichi virus 1 in children admitted to hospitals with acute gastroenteritis in Chiang Mai, Thailand. A total of 923 fecal specimens collected from January, 2011 to December, 2013 were screened for the presence of Aichi virus 1 by RT semi-nested PCR. Out of 923 fecal specimens tested, Aichi virus 1 was detected with the prevalence of 2.6% (24/923). Of these, 0.3% (3/923) was genotype A and 2.3% (21/923) were genotype B. It is interesting to note that the genotype A showed the nucleotide sequence closely related to the Aichi virus reference strain isolated from sewage in Tunisia, while genotype B was most closely related to other human Aichi virus B reference strains. The results suggest that Aichi virus 1 of both genotypes A and B are circulating in pediatric patients in Thailand. J. Med. Virol. 89:234-238, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Faculty of Medicine, Department of Microbiology, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Watchaporn
authors.initials: W
authors.lastname: Chuchaona
authors.affiliation: Faculty of Medicine, Department of Microbiology, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Pattara
authors.initials: P
authors.lastname: Khamrin
authors.affiliation: Faculty of Medicine, Department of Microbiology, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Arpaporn
authors.initials: A
authors.lastname: Yodmeeklin
authors.affiliation: Faculty of Medicine, Department of Microbiology, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Kattareeya
authors.initials: K
authors.lastname: Kumthip
authors.affiliation: Faculty of Medicine, Department of Microbiology, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Wilaiporn
authors.initials: W
authors.lastname: Saikruang
authors.affiliation: Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.|Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Aksara
authors.initials: A
authors.lastname: Thongprachum
authors.affiliation: Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.|Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Okitsu
authors.affiliation: Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.|Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
authors.affiliation: Faculty of Medicine, Department of Microbiology, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Niwat
authors.initials: N
authors.lastname: Maneekarn
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006760:Hospitalization; D006801:Humans; D007223:Infant; D052699:Kobuvirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D010850:Picornaviridae Infections / Q000453:epidemiology / Q000821:virology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D013785:Thailand / Q000453:epidemiology
keywords: Aichi virus 1; Thailand; children; diarrhea; genotype