title: Differential Adhesion Selection for Enrichment of Tendon-Derived Progenitor Cells During In Vitro Culture.
abstract: Preplating, a technique used to separate rapidly adherent fibroblasts from the less-adherent progenitor cells, has been used successfully to isolate skeletal muscle-derived stem cells. The objective of this study was to determine if preplating could also be applied to enrich tendon-derived progenitor cells (TDPCs) before monolayer expansion. Cell suspensions obtained by collagenase digestion of equine lateral digital extensor tendon were serially transferred into adherent plates every 12 h for 4 days. TDPC fractions obtained from initial (TPP0), third (TPP3), and seventh (TPP7) preplate were passaged twice and used for subsequent analyses. Growth/proliferation and basal tenogenic gene expression of the three TDPC fractions were largely similar. Preplating and subsequent monolayer expansion did not alter the immunophenotype (CD29(+), CD44(+), CD90(+), and CD45(-)) and trilineage differentiation capacity of TDPC fractions. Overall, TDPCs were robustly osteogenic, but exhibited comparatively weak adipogenic and chondrogenic capacities. These outcomes indicate that preplating does not enrich for tendon-derived progenitors during in vitro culture, and "whole tendon digest"-derived cells are as appropriate for cell-based therapies.
authors.affiliation: Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois , Urbana, Illinois.
authors.forename: Sushmitha
authors.initials: S
authors.lastname: Durgam
authors.affiliation: Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois , Urbana, Illinois.
authors.forename: Brooke
authors.initials: B
authors.lastname: Schuster
authors.affiliation: Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois , Urbana, Illinois.
authors.forename: Anna
authors.initials: A
authors.lastname: Cymerman
authors.affiliation: Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois , Urbana, Illinois.
authors.forename: Allison
authors.initials: A
authors.lastname: Stewart
authors.affiliation: Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois , Urbana, Illinois.
authors.forename: Matthew
authors.initials: M
authors.lastname: Stewart
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D020219:Chondrogenesis; D006736:Horses; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D013710:Tendons / Q000166:cytology* / Q000502:physiology