title: Collateral projections from the lateral parabrachial nucleus to the paraventricular thalamic nucleus and the central amygdaloid nucleus in the rat.
abstract: Combined the retrograde double tracing with immunofluorescence histochemical staining, we examined the neurons in the lateral parabrachial nucleus (LPB) sent collateral projections to the paraventricular thalamic nucleus (PVT) and central amygdaloid nucleus (CeA) and their roles in the nociceptive transmission in the rat. After the injection of Fluoro-gold (FG) into the PVT and tetramethylrhodamine-dextran (TMR) into the CeA, respectively, FG/TMR double-labeled neurons were observed in the LPB. The percentages of FG/TMR double-labeled neurons to the total number of FG- or TMR-labeled neurons were 6.18% and 9.09%, respectively. Almost all of the FG/TMR double-labeled neurons (95%) exhibited calcitonin gene-related peptide (CGRP) immunoreactivity. In the condition of neuropathic pain, 94% of these neurons showed FOS immunoreactivity. The present data indicates that some of CGRP-expressing neurons in the LPB may transmit nociceptive information toward the PVT and CeA by way of axon collaterals.
authors.affiliation: Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350108, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Liang
authors.affiliation: Department of Anatomy, Histology and Embryology and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jun-Bin
authors.initials: JB
authors.lastname: Yin
authors.affiliation: Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350108, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Anatomy, Histology and Embryology and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Anatomy, Histology and Embryology and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; Student Brigade, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Kai-Xiang
authors.initials: KX
authors.lastname: Zhou
authors.affiliation: Department of Anatomy, Histology and Embryology and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; Student Brigade, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Zhao
authors.affiliation: Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350108, China. Electronic address: wangwei0976@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Anatomy, Histology and Embryology and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China. Electronic address: donganat@fmmu.edu.cn.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Dong
authors.affiliation: Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350108, China; Department of Anatomy, Histology and Embryology and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China. Electronic address: deptanat@fmmu.edu.cn.
authors.forename: Yun-Qing
authors.initials: YQ
authors.lastname: Li
mesh_terms: D000818:Animals; D001369:Axons; D066274:Central Amygdaloid Nucleus / Q000166:cytology*; D008297:Male; D020644:Midline Thalamic Nuclei / Q000166:cytology*; D009434:Neural Pathways / Q000166:cytology; D056347:Neuroanatomical Tract-Tracing Techniques; D009474:Neurons / Q000166:cytology*; D065823:Parabrachial Nucleus / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley
keywords: Calcitonin gene-related peptide; Central amygdaloid nucleus; Collateral projection; FOS; Lateral parabrachial nucleus; Paraventricular thalamic nucleus