title: Fecal Microbiota and Diet of Children with Chronic Constipation.
abstract: Many factors explain dysbiosis in chronic constipation (CC), such as a low-fiber diet. The objective of this study was to compare the fecal microbiota of constipated and nonconstipated children and their intake frequencies of food. Methods. This observational study included 79 children (M/F 43/36) aged six to 36 months divided into two groups: cases (39 constipated children) and controls (40 nonconstipated children). We used a structured form to collect demographic variables, conducted anthropometric assessment, and collected food intake frequency data. The fecal microbiota of the stool samples was analyzed by real-time polymerase chain reaction (PCR) using the fluorophore SYBR® Green. Results. Constipated children had a smaller concentration of Lactobacillus per milligram of stool (p = 0.015) than nonconstipated children, but the concentration of Bifidobacterium per milligram of stool (p = 0.323) and the intake of fruits, vegetables (p = 0.563), and junk food (p = 0.093) of the two groups did not differ. Constipated children consumed more dairy products (0.45 ± 0.8; p > 0.001), were more frequently delivered via caesarean section (69.2%), were weaned earlier (median: 120; 60Q1-240Q3), and had a family history of constipation (71.8%). Conclusions. Children with CC have a smaller concentration of Lactobacillus in their stools and consume more dairy products.
authors.affiliation: Federal University of Pernambuco, Avenida Professor Moraes Rego 1235, University City, 50670-901 Recife, PE, Brazil.
authors.forename: Joyce Gomes
authors.identifier: 0000-0003-2222-6185
authors.initials: JG
authors.lastname: de Moraes
authors.affiliation: Mother-Child Department, Federal University of Pernambuco, Avenida Professor Moraes Rego 1235, University City, 50670-901 Recife, PE, Brazil.
authors.forename: Maria Eugênia Farias de Almeida
authors.initials: ME
authors.lastname: Motta
authors.affiliation: Department of Biotechnology, Laboratory of Immunopathology Keizo Asami (LIKA), Avenida Professor Moraes Rego 1235, University City, 50670-901 Recife, PE, Brazil.
authors.forename: Monique Ferraz de Sá
authors.initials: MF
authors.lastname: Beltrão
authors.affiliation: Department of Biotechnology, Laboratory of Immunopathology Keizo Asami (LIKA), Avenida Professor Moraes Rego 1235, University City, 50670-901 Recife, PE, Brazil.
authors.forename: Taciana Lima
authors.initials: TL
authors.lastname: Salviano
authors.affiliation: Mother-Child Department, Federal University of Pernambuco, Avenida Professor Moraes Rego 1235, University City, 50670-901 Recife, PE, Brazil.
authors.forename: Giselia Alves Pontes
authors.identifier: 0000-0002-5310-3420
authors.initials: GA
authors.lastname: da Silva