title: The effect of acute ophiobolin A treatment on HO-mediated inflammatory processes.
abstract: Many microbial and plant-derived metabolites contribute to the production of inflammatory mediators and the expression of pro-inflammatory molecules. Ophiobolin A (OPA) is a fungal secondary metabolite produced by Bipolaris species. The aim of our study was to examine the acute effects of this compound on inflammatory processes. Male Wistar rats were treated with 5% ethanol, 0.01 mg/kg OPA, 0.1 mg/kg OPA and 1.0 mg/kg OPA per os. After 24 h of the administrations, inflammatory mediators such as interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-α) and myeloperoxidase (MPO) enzyme as well as heme oxygenase (HO) activity were measured in both plasma and cardiac tissue, along with serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). We found that OPA caused a significant elevation in the concentrations of IL-6 and TNF-α, increased MPO activity and decreased HO enzyme activity in the plasma. While OPA induces inflammation in the plasma, it did not change the level of inflammatory mediators in the cardiac tissue and the concentrations of serum ALT and AST. Our findings indicate that rapid release of inflammatory mediators by OPA promotes systemic inflammation. However, this acute OPA treatment does not show toxic effects on the cardiac tissue and the concentrations of liver enzymes.
authors.affiliation: 1 Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: Anikó
authors.initials: A
authors.lastname: Pósa
authors.affiliation: 1 Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: Renáta
authors.initials: R
authors.lastname: Szabó
authors.affiliation: 1 Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: Zita
authors.initials: Z
authors.lastname: Szalai
authors.affiliation: 1 Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Kupai
authors.affiliation: 1 Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Deim
authors.affiliation: 2 College of Arts and Sciences, Qatar University, Qatar.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Murlasits
authors.affiliation: 3 Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: Ottó
authors.initials: O
authors.lastname: Bencsik
authors.affiliation: 3 Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Szekeres
authors.affiliation: 3 Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Vágvölgyi
authors.affiliation: 4 Institute of Physical Education and Sport Science, Juhász Gyula Faculty of Education, University of Szeged, Szeged, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Balogh
authors.affiliation: 5 Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Béla
authors.initials: B
authors.lastname: Juhász
authors.affiliation: 5 Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szilvássy
authors.affiliation: 1 Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Varga
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000097:blood / Q000378:metabolism; D007249:Inflammation / Q000097:blood / Q000139:chemically induced; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000097:blood / Q000378:metabolism; D017208:Rats, Wistar; D054830:Sesterterpenes / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D054830:Sesterterpenes; D014409:Tumor Necrosis Factor-alpha; C040764:ophiobolin A; D009195:Peroxidase; D006419:Heme Oxygenase (Decyclizing); D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Ophiobolin A (OPA); heme oxygenase; inflammation; myeloperoxidase; pro-inflammatory cytokines