title: Lectins from Parkia biglobosa and Parkia platycephala: A comparative study of structure and biological effects.
abstract: The relation structure-activity of the Mimosoideae lectins of Parkia platycephala (PPL) and Parkia biglobosa (PBL) was analyzed in this study. PBL was solved by X-ray crystallography at a resolution of 2.1Å, and the crystal structure belonged to the C2221space group. Structural organization and binding sites were also characterized. Specifically, PBL monomer consists of three β-prism domains tandemly arranged with each one presenting a different carbohydrate recognition domain (CRD). PPL showed antinociceptive activity in the mouse model of acetic acid-induced writhes with maximal inhibitory effect by 74% at 1mg/mL. PPL also demonstrated anti-inflammatory effect causing inhibition of leukocyte migration induced by both direct and indirect chemoattractants. These PPL activities were compared to that of PBL described previously. Molecular docking of both PBL and PPL demonstrated some differences in carbohydrate-lectin interaction energy. Comparing structure and biological effects of the two lectins provided new data about their structure and the relation with its biological activities.
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil.
authors.forename: Alfa Umaro
authors.initials: AU
authors.lastname: Bari
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil.
authors.forename: Mayara Queiroz
authors.initials: MQ
authors.lastname: Santiago
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil.
authors.forename: Vinicius Jose Silva
authors.initials: VJ
authors.lastname: Osterne
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil.
authors.forename: Vanir Reis
authors.initials: VR
authors.lastname: Pinto-Junior
authors.affiliation: Laboratório de Fisiofarmacologia da Inflamação, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, 60714-242, Fortaleza, Ceará, Brazil.
authors.forename: Lívia Paulo
authors.initials: LP
authors.lastname: Pereira
authors.affiliation: Departamento de Biologia Molecular, Universidade Federal da Paraíba, Paraíba, Brazil.
authors.forename: Jose Caetano
authors.initials: JC
authors.lastname: Silva-Filho
authors.affiliation: University of Science and Technology of Lille, Lille, France.
authors.forename: Henri
authors.initials: H
authors.lastname: Debray
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil.
authors.forename: Bruno Anderson Matias
authors.initials: BA
authors.lastname: Rocha
authors.affiliation: Departamento de Biologia Molecular, Universidade Federal da Paraíba, Paraíba, Brazil.
authors.forename: Plinio
authors.initials: P
authors.lastname: Delatorre
authors.affiliation: Centro de Ciências Agrarias e Ambientais, Universidade Federal do Maranhão, Maranhão, Brazil.
authors.forename: Claudener Souza
authors.initials: CS
authors.lastname: Teixeira
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil.
authors.forename: Cornevile Correia
authors.initials: CC
authors.lastname: Neto
authors.affiliation: Laboratório de Fisiofarmacologia da Inflamação, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, 60714-242, Fortaleza, Ceará, Brazil.
authors.forename: Ana Maria Sampaio
authors.initials: AM
authors.lastname: Assreuy
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil.
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: Nascimento
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil. Electronic address: bscavada@ufc.br.
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D000595:Amino Acid Sequence; D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001665:Binding Sites; D002465:Cell Movement / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry* / Q000494:pharmacology*; D007962:Leukocytes / Q000166:cytology; D051379:Mice; D062105:Molecular Docking Simulation; D000072417:Protein Domains; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D055672:Static Electricity
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D037102:Lectins
keywords: Biological effects; Crystal structure; Parkia lectin