title: Toxicity of serum albumin on microglia upon seeding effect of amyloid peptide.
abstract: We demonstrate in vitro cross-seeding of bovine serum albumin (BSA) in the presence of Aβ25-35and their cytotoxic effects on microglial cells. To investigate the cross-seeding of BSA in the presence of Aβ25-35fibrils, we examined how Aβ25-35fibrils can function as seeds to trigger and accelerate BSA fibrillogenesis using ThT, intrinsic fluorescence, ANS fluorescence and transmission electron microscopy (TEM). Moreover, the effects of these fibrils on microglial viability were measured using MTT and Annexin V/propidium iodide (PI) staining. Although Aβ25-35is toxic against microglia, it acted as seed and affected the aggregation pathway and accelerated the fibrillogenesis of BSA in vitro, resulted in an enhanced cytotoxic effect in comparison with Aβ25-35or BSA alone. These observations thought to be helpful to understand the molecular mechanism of enhanced toxicity due to the coexistence of the aggregation prone proteins/peptides,. then cross-seeding effect on microglial cells that may involve in neurodegenerative diseases such as Alzheimer's disease (AD).
authors.affiliation: School of Biology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ferdousi
authors.affiliation: School of Biology, College of Science, University of Tehran, Tehran, Iran mhabibi@ut.ac.ir.|Nano-Biomedicine Center of Excellence, Nanoscience and Nanotechnology Research Center, University of Tehran, Tehran, Iran.
authors.forename: Mehran
authors.initials: M
authors.lastname: Habibi-Rezaei
authors.affiliation: Peptide Chemistry Research Center, K. N. Toosi University of Technology, Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Balalaie
authors.affiliation: Peptide Chemistry Research Center, K. N. Toosi University of Technology, Tehran, Iran.
authors.forename: Sorour
authors.initials: S
authors.lastname: Ramezanpour
authors.affiliation: Department of Basic Sciences of Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Sabouni
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Najmeh
authors.initials: N
authors.lastname: Poursasan
authors.affiliation: School of Biology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Manijheh
authors.initials: M
authors.lastname: Sabokdast
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.|Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran.
authors.forename: Ali A
authors.initials: AA
authors.lastname: Moosavi-Movahedi
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology*; D017628:Microglia / Q000378:metabolism* / Q000473:pathology; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D016229:Amyloid beta-Peptides; D003603:Cytotoxins; D010446:Peptide Fragments; C000604414:amyloid beta-protein (23-35); D012710:Serum Albumin, Bovine
keywords: Alzheimer’s disease; Aβ25-35
  ; cross-seeding; microglia; serum albumin