title: Hormones in Foods: Presence of Enterostatin-Like Immunoreactivities in Bovine Milk.
abstract: Enterostatins, pentapeptides (Val-Pro-Asp-Pro-Arg [VPDPR], Val-Pro-Gly-Pro-Arg, Ala-Pro-Gly-Pro- Arg [APGPR], and others) derived from the amino terminus of procolipase, are endogenous to a variety of tissues and body fluids including brain, gut, blood, cerebrospinal fluid, and urine. The administration of exogenous peptides has been shown to elicit a variety of biologic activities, including a decrease in dietary fat preference and pancreatic insulin secretion. Since milk is a rich source of a variety of bioactive substances, especially peptides, we investigated the presence of enterostatin-like immunoreactivity in bovine milk. We measured enterostatins-APGPR and VPDPR-in milk from a herd of 19 cows randomly selected from the Louisiana State University Department of Dairy Science Research Herd in Baton Rouge; the results of this study show a mean peptide concentration in raw milk of 33.7 ± 2.9 ng/ml for APGPR and of 104.5 ± 16.3 ng/ml for VPDPR. A further chromatographic characterization of the nature of APGPR- and VPDPR-like immunoreactivities suggested the endogenous peptides share a common epitope with APGPR or VPDPR but are not APGPR or VPDPR. Unlike APGPR or VPDPR, the endogenous peptides were heat-labile and therefore their values were much lower in pasteurized milk.
authors.affiliation: a Section of Endocrinology, Obesity Research Program, Department of Medicine , LSU Medical Center , 1542 Tulane Avenue, New Orleans , LA 70112 , USA.
authors.forename: C
authors.initials: C
authors.lastname: Prasad
authors.affiliation: a Section of Endocrinology, Obesity Research Program, Department of Medicine , LSU Medical Center , 1542 Tulane Avenue, New Orleans , LA 70112 , USA.
authors.forename: C
authors.initials: C
authors.lastname: Debata
authors.affiliation: b Department of Dairy Science , Louisiana State University , Baton Rouge , LA 70803 , USA.
authors.forename: J U
authors.initials: JU
authors.lastname: McGregor
keywords: Ala-Pro-Gly-Pro-Arg; Cow's milk; Enterostatins; Val-Pro-Asp-Pro-Arg; Val-Pro-Gly-Pro-Arg