title: Commensal Bacteria Aid Mate-selection in the Fruit Fly, Bactrocera dorsalis.
abstract: Commensal bacteria influence many aspects of an organism's behaviour. However, studies on the influence of commensal bacteria in insect mate-selection are scarce. Here, we present empirical evidence that commensal bacteria mediate mate-selection in the Oriental fruit fly, Bactrocera dorsalis. Male flies were attracted to female flies, but this attraction was abolished when female flies were fed with antibiotics, suggesting the role of the fly's microbiota in mediating mate-selection. We show that male flies were attracted to and ejaculated more sperm into females harbouring the microbiota. Using culturing and 16S rDNA sequencing, we isolated and identified different commensal bacteria, with Klebsiella oxytoca being the most abundant bacterial species. This preliminary study will enhance our understanding of the influence of commensal bacteria on mate-selection behaviour of B. dorsalis and may find use in devising control operations against this devastating pest.
authors.affiliation: National Fellow Lab, Division of Entomology and Nematology, Indian Institute of Horticultural Research, Hesserghatta Lake Post, Bangalore, 560 089, India.
authors.forename: Kamala Jayanthi Pagadala
authors.initials: KJ
authors.lastname: Damodaram
authors.affiliation: National Fellow Lab, Division of Entomology and Nematology, Indian Institute of Horticultural Research, Hesserghatta Lake Post, Bangalore, 560 089, India.
authors.forename: Arthikirubha
authors.initials: A
authors.lastname: Ayyasamy
authors.affiliation: National Fellow Lab, Division of Entomology and Nematology, Indian Institute of Horticultural Research, Hesserghatta Lake Post, Bangalore, 560 089, India. vivek.kempraj@gmail.com.
authors.forename: Vivek
authors.initials: V
authors.lastname: Kempraj
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D018407:Bacterial Physiological Phenomena / Q000187:drug effects; D001483:Base Sequence; D004275:DNA, Ribosomal; D004542:Ejaculation; D005260:Female; D041121:Klebsiella oxytoca / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D008393:Marriage; D064307:Microbiota; D064367:Olfactometry / Q000379:methods; D012726:Sexual Behavior, Animal / Q000187:drug effects; D013076:Sperm Count; D013094:Spermatozoa; D013559:Symbiosis; D033621:Tephritidae / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D004275:DNA, Ribosomal
keywords: B. dorsalis; Commensal bacteria; Klebsiella oxytoca; Mate-selection