title: Electron transfer of Pseudomonas aeruginosa CP1 in electrochemical reduction of nitric oxide.
abstract: This study reports catalytic electro-chemical reduction of nitric oxide (NO) enhanced by Pseudomonas aeruginosa strain CP1. The current generated in the presence of bacteria was 4.36times that in the absence of the bacteria. The strain was able to catalyze electro-chemical reduction of NO via indirect electron transfer with an electrode, revealed by a series of cyclic voltammetry experiments. Soluble electron shuttles secreted into solution by live bacteria were responsible for the catalytic effects. The enhancement of NO reduction was also confirmed by detection of nitrous oxide; the level of this intermediate was 46.4% higher in the presence of bacteria than in controls, illustrated that the electron transfer pathway did not directly reduce nitric oxide to N2. The findings of this study may offer a new model for bioelectrochemical research in the field of NO removal by biocatalysts.
authors.affiliation: School of Environment and Energy, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, Guangzhou 510006, PR China.
authors.forename: Shaofeng
authors.initials: S
authors.lastname: Zhou
authors.affiliation: School of Environment and Energy, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, Guangzhou 510006, PR China. Electronic address: chshuang@scut.edu.cn.
authors.forename: Shaobin
authors.initials: S
authors.lastname: Huang
authors.affiliation: School of Environment and Energy, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, Guangzhou 510006, PR China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: He
authors.affiliation: School of Environment and Energy, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, Guangzhou 510006, PR China.
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Environment and Energy, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, Guangzhou 510006, PR China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D055664:Electrochemical Techniques / Q000379:methods*; D004579:Electron Transport; D009569:Nitric Oxide / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D009609:Nitrous Oxide / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000378:metabolism
chemical_list: D009569:Nitric Oxide; D009609:Nitrous Oxide
keywords: Denitrification; Electron transfer; Nitric oxide reduction; Pseudomonas aeruginosa CP1; Soluble shuttles