title: Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of KPC-Producing Klebsiella pneumoniae.
abstract: We evaluated a real-time single-peak (11.109-Da) detection assay based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the identification of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae Our results demonstrated that the 11.109-Da peak was detected in 88.2% of the KPC producers. Analysis of blaKPC-producing K. pneumoniae showed that the gene encoding the 11.109-Da protein was commonly (97.8%) associated with the Tn4401a isoform.
authors.affiliation: Operative Unit of Clinical Microbiology, S. Orsola-Malpighi University Hospital, Bologna, Italy paolo.gaibani@unibo.it.
authors.forename: Paolo
authors.initials: P
authors.lastname: Gaibani
authors.affiliation: Operative Unit of Clinical Microbiology, S. Orsola-Malpighi University Hospital, Bologna, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Galea
authors.affiliation: Bruker Daltonics s.r.l., Macerata, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Fagioni
authors.affiliation: Operative Unit of Clinical Microbiology, S. Orsola-Malpighi University Hospital, Bologna, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Ambretti
authors.affiliation: Unit of Microbiology, The Greater Romagna Area Hub Laboratory, Pievesestina, Italy DIMES, University of Bologna, Bologna, Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Sambri
authors.affiliation: Operative Unit of Clinical Microbiology, S. Orsola-Malpighi University Hospital, Bologna, Italy DIMES, University of Bologna, Bologna, Italy.
authors.forename: Maria Paola
authors.initials: MP
authors.lastname: Landini
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D005798:Genes, Bacterial; D006801:Humans; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D001618:beta-Lactamases / Q000032:analysis* / Q000737:chemistry / Q000235:genetics
chemical_list: D001618:beta-Lactamases