title: Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis.
abstract: Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans and animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and other members of the MTC evolved. The genome of M. bovis is over >99.95% identical to that of M. tuberculosis but with seven deletions ranging in size from 1 to 12.7 kb. In addition, 1200 single nucleotide mutations in coding regions distinguish M. bovis from M. tuberculosis. In the present study, we assessed 75 M. tuberculosis genomes and 23 M. bovis genomes to identify non-synonymous mutations in 202 coding sequences of regulatory genes between both species. We identified species-specific variants in 20 regulatory proteins and confirmed differential expression of hypoxia-related genes between M. bovis and M. tuberculosis.
authors.affiliation: School of Agronomy, UBA, Buenos Aires 1417, Argentina.
authors.forename: María M
authors.initials: MM
authors.lastname: Bigi
authors.affiliation: Biotechnology Institute, National Institute of Agricultural Technology (INTA), Hurlingham 1686, Argentina.
authors.forename: Federico Carlos
authors.initials: FC
authors.lastname: Blanco
authors.affiliation: EMBRAPA, Mato Grosso do Sul 8605, Brazil.
authors.forename: Flabio R
authors.initials: FR
authors.lastname: Araújo
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, 1920 Dayton Ave, Ames, Iowa 50010, USA.
authors.forename: Tyler C
authors.initials: TC
authors.lastname: Thacker
authors.affiliation: Biotechnology Institute, National Institute of Agricultural Technology (INTA), Hurlingham 1686, Argentina.
authors.forename: Martín J
authors.initials: MJ
authors.lastname: Zumárraga
authors.affiliation: Biotechnology Institute, National Institute of Agricultural Technology (INTA), Hurlingham 1686, Argentina.
authors.forename: Angel A
authors.initials: AA
authors.lastname: Cataldi
authors.affiliation: School of Agronomy, UBA, Buenos Aires 1417, Argentina.
authors.forename: Marcelo A
authors.initials: MA
authors.lastname: Soria
authors.affiliation: Biotechnology Institute, National Institute of Agricultural Technology (INTA), Hurlingham 1686, Argentina.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Bigi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D002417:Cattle; D019295:Computational Biology / Q000379:methods; D019143:Evolution, Molecular; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006801:Humans; D009154:Mutation; D009163:Mycobacterium bovis / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D011110:Polymorphism, Genetic; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D014376:Tuberculosis / Q000382:microbiology; D014380:Tuberculosis, Bovine / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D014157:Transcription Factors
keywords: Mycobacterium bovis; Mycobacterium tuberculosis; polymorphism; regulator