title: Tuning the Catalytic Activity of Subcellular Nanoreactors.
abstract: Bacterial microcompartments are naturally occurring subcellular organelles of bacteria and serve as a promising scaffold for the organization of heterologous biosynthetic pathways. A critical element in the design of custom biosynthetic organelles is quantitative control over the loading of heterologous enzymes to the interior of the organelles. We demonstrate that the loading of heterologous proteins to the 1,2-propanediol utilization microcompartment of Salmonella enterica can be controlled using two strategies: by modulating the transcriptional activation of the microcompartment container and by coordinating the expression of the microcompartment container and the heterologous cargo. These strategies allow general control over the loading of heterologous proteins localized by two different N-terminal targeting peptides and represent an important step toward tuning the catalytic activity of bacterial microcompartments for increased biosynthetic productivity.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720, USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Jakobson
authors.affiliation: Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720, USA.
authors.forename: Marilyn F
authors.initials: MF
authors.lastname: Slininger
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
authors.forename: Elias
authors.initials: E
authors.lastname: Valdivia
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720, USA.
authors.forename: Edward Y
authors.initials: EY
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA. Electronic address: dtercek@berkeley.edu.
authors.forename: Danielle
authors.initials: D
authors.lastname: Tullman-Ercek
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002384:Catalysis; D015388:Organelles / Q000378:metabolism* / Q000502:physiology*; D019946:Propylene Glycol / Q000378:metabolism; D019779:Salmonella enterica / Q000378:metabolism / Q000502:physiology; D015533:Transcriptional Activation / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D019946:Propylene Glycol
keywords: 1,2-propanediol utilization; Salmonella; bacterial microcompartments; subcellular organelles; synthetic biology