title: Traumatic Acid Reduces Oxidative Stress and Enhances Collagen Biosynthesis in Cultured Human Skin Fibroblasts.
abstract: Traumatic acid (TA) is a plant hormone (cytokinin) that in terms of chemical structure belongs to the group of fatty acids derivatives. It was isolated from Phaseolus vulgaris. TA activity and its influence on human cells and organism has not previously been the subject of research. The aim of this study was to examine the effects of TA on collagen content and basic oxidative stress parameters, such as antioxidative enzyme activity, reduced glutathione, thiol group content, and lipid peroxidation in physiological conditions. The results show a stimulatory effect of TA on tested parameters. TA caused a decrease in membrane phospholipid peroxidation and exhibited protective properties against ROS production. It also increases protein and collagen biosynthesis and its secretion into the culture medium. The present findings reveal that TA exhibits multiple and complex activity in fibroblast cells in vitro. TA, with its activity similar to unsaturated fatty acids, shows antioxidant and stimulatory effects on collagen biosynthesis. It is a potentially powerful agent with applications in the treatment of many skin diseases connected with oxidative stress and collagen biosynthesis disorders.
authors.affiliation: Faculty of Civil and Environmental Engineering, Division of Sanitary Biology and Biotechnology, Bialystok University of Technology, Wiejska Street 45E, 15-351, Białystok, Poland. a.jablonska@pb.edu.pl.
authors.forename: Agata
authors.initials: A
authors.lastname: Jabłońska-Trypuć
authors.affiliation: University of Medical Science in Białystok, Krakowska Street 9, 15-875, Białystok, Poland.
authors.forename: Walentyn
authors.initials: W
authors.lastname: Pankiewicz
authors.affiliation: University of Medical Science in Białystok, Krakowska Street 9, 15-875, Białystok, Poland.
authors.forename: Romuald
authors.initials: R
authors.lastname: Czerpak
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D003998:Dicarboxylic Acids / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010743:Phospholipids / Q000378:metabolism; D012867:Skin / Q000166:cytology* / Q000187:drug effects
chemical_list: D003998:Dicarboxylic Acids; D010743:Phospholipids; D003094:Collagen; C509089:traumatic acid
keywords: Catalase; Collagen; Cytokinins; Glutathione; Glutathione peroxidase; Glutathione reductase; Lipid peroxidation; Traumatic acid