title: Structural analysis of Pseudomonas aeruginosa H3-T6SS immunity proteins.
abstract: The Pseudomonas aeruginosa PldB protein is a transkingdom effector secreted by the Type VI Secretion System (T6SS). PA5088, PA5087, and PA5086 are three immunity proteins that can suppress the virulence of PldB. We report the crystal structures of PA5088 and PA5087 at 2.0 and 2.1 Å resolution, respectively. PA5088 and PA5087 both consist of several Sel1-like Repeats (SLRs) and form super-ring folds. Our structural analysis of these proteins revealed key differences among PA5088, PA5087, and their homologs. Our docking experiments have shed light on the putative interaction mechanism of their function as phospholipase D inhibitors.
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Yang
authors.affiliation: College of Life Science and Technology, Huazhong Agriculture University, Wuhan, China.
authors.forename: Zong-Qiang
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: Multidiscipline Research Center, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhun
authors.initials: Z
authors.lastname: She
authors.affiliation: Multidiscipline Research Center, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Geng
authors.affiliation: Multidiscipline Research Center, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Xu
authors.affiliation: Multidiscipline Research Center, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zeng-Qiang
authors.initials: ZQ
authors.lastname: Gao
authors.affiliation: Multidiscipline Research Center, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Dong
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000627:therapeutic use; D058947:Bacterial Secretion Systems / Q000737:chemistry* / Q000235:genetics; D018360:Crystallography, X-Ray; D010739:Phospholipase D / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation / Q000187:drug effects; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D010739:Phospholipase D
keywords: Sel1-like repeats; immunity protein; molecular docking; super-ring; type VI secretion system