title: Invariant asymmetry renews the lymphatic vasculature during homeostasis.
abstract: The lymphatic vasculature regulates tissue physiology and immunity throughout life. The self renewal mechanism that maintains the lymphatic vasculature during conditions of homeostasis is unknown. The purpose of this study was to investigate the cellular mechanism of lymphatic endothelial cell (LEC) self renewal and lymphatic vessel maintenance.
  
  
  
  Inductive genetic techniques were used to label LECs with tandem dimer tomato (tdT) in adult mice. Two types of studies were performed, those with high dose inductive conditions to label nearly all the lymphatic vessels and studies with low dose inductive conditions to stochastically label individual clones or small populations of LECs. We coupled image guidance techniques and live fluorescence microscopy imaging with lineage tracing to track the fate of entire tdT(+) cutaneous lymphatic vessels or the behavior of individual or small populations of LECs over 11 months. We tracked the fate of 110 LEC clones and 80 small LEC populations (clusters of 2-7 cells) over 11 months and analyzed their behavior using quantitative techniques.
  
  
  
  The results of the high dose inductive studies showed that the lymphatic vessels remained tdT(+) over 11 months, suggesting passage and expression of the tdT transgene from LEC precursors to progenies, an intrinsic model of self- renewal. Interestingly, the morphology of tdT(+) lymphatic vasculature appeared relatively stable without significant remodeling during this time period. By following the behavior of labeled LEC clones or small populations of LECs individually over 11 months, we identified diverse LEC fates of proliferation, quiescence, and extinction. Quantitative analysis of this data revealed that the average lymphatic endothelial clone or small population remained stable in size despite diverse individual fates.
  
  
  
  The results of these studies support a mechanism of invariant asymmetry to self renew the lymphatic vasculature during homeostasis. These original findings raise important questions related to the plasticity and self renewal properties that maintain the lymphatic vasculature during life.
authors.affiliation: Department of Neurosensory Genetics and Otolaryngology and Head and Neck Surgery, Boys Town National Research Hospital, 555 North 30th Street, Omaha, NE, 68131, USA.
authors.forename: Alicia L
authors.initials: AL
authors.lastname: Connor
authors.affiliation: Department of Neurosensory Genetics and Otolaryngology and Head and Neck Surgery, Boys Town National Research Hospital, 555 North 30th Street, Omaha, NE, 68131, USA.
authors.forename: Philip M
authors.initials: PM
authors.lastname: Kelley
authors.affiliation: Department of Neurosensory Genetics and Otolaryngology and Head and Neck Surgery, Boys Town National Research Hospital, 555 North 30th Street, Omaha, NE, 68131, USA. richard.tempero@boystown.org.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Tempero
mesh_terms: D000818:Animals; D019070:Cell Lineage; D049109:Cell Proliferation; D002999:Clone Cells; D042783:Endothelial Cells / Q000166:cytology; D005453:Fluorescence; D006706:Homeostasis; D021621:Imaging, Three-Dimensional; D019426:Integrases / Q000378:metabolism; D042601:Lymphatic Vessels / Q000166:cytology / Q000502:physiology*; D008822:Mice, Transgenic; D008954:Models, Biological
chemical_list: C045073:Cre recombinase; D019426:Integrases