title: Impaired CD23 and CD62L expression and tissue inhibitors of metalloproteinases secretion by eosinophils in adults with atopic dermatitis.
abstract: BACKGROUND
  Eosinophils are multifunctional, polymorphonuclear leucocytes that secrete proteins within cytoplasmic granules, such as cytokines, chemokines, metalloproteinases (MMPs) and metalloproteinases tissue inhibitors (TIMPs). Although eosinophilia is a hallmark of atopic dermatitis (AD), several functional aspects of eosinophils remain unknown.
  
  
  OBJECTIVE
  We aimed to evaluate the phenotype and functional response of eosinophils under staphylococcal enterotoxin B (SEB) and Toll-like receptor (TLR)-2/6 (FSL-1) stimulation in the secretion of CCL5, MMPs and TIMPs in adults with AD.
  
  
  METHODS
  Forty-one adult patients with AD and 45 healthy controls enrolled for the study. Phenotype of eosinophils from granulocytes of peripheral blood was analysed by flow cytometry. We performed evaluation of CCL5 (cytometric bead array), MMP and TIMP (ELISA) secretion, in culture supernatants of purified eosinophils stimulated with SEB or TLR2/6 agonist (FSL-1).
  
  
  RESULTS
  We found a higher frequency of LIN1-CCR3+eosinophils, and decreased expression of CD23 and CD62L receptors in eosinophils of AD patients. There was no difference in MMP and TIMP serum levels between the evaluated groups. However, we detected decreased basal levels of TIMP-1, TIMP-2 and CCL5 in culture supernatants from purified, unstimulated eosinophils from AD patients.
  
  
  CONCLUSIONS
  In adults with AD, phenotypical features of eosinophils reveal decreased expression of early activation and L-selectin receptors. Regarding the functional profile of purified eosinophils related to tissue remodelling in atopic dermatitis, innate immune stimulation (TLR2/6 agonist and SEB) did not affect the ratio of MMP/TIMPs secretion in AD. Our findings reinforce the potential breakdown in tissue remodelling process mediated by eosinophils in AD.
authors.affiliation: Laboratory of Dermatology and Immunodeficiency (LIM-56), Department of Dermatology, University of Sao Paulo Medical School, Sao Paulo, SP, Brazil.
authors.forename: T
authors.initials: T
authors.lastname: de Oliveira Titz
authors.affiliation: Laboratory of Dermatology and Immunodeficiency (LIM-56), Department of Dermatology, University of Sao Paulo Medical School, Sao Paulo, SP, Brazil.
authors.forename: R L
authors.initials: RL
authors.lastname: Orfali
authors.affiliation: Laboratory of Dermatology and Immunodeficiency (LIM-56), Department of Dermatology, University of Sao Paulo Medical School, Sao Paulo, SP, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: de Lollo
authors.affiliation: Laboratory of Dermatology and Immunodeficiency (LIM-56), Department of Dermatology, University of Sao Paulo Medical School, Sao Paulo, SP, Brazil.
authors.forename: V G
authors.initials: VG
authors.lastname: Dos Santos
authors.affiliation: Laboratory of Dermatology and Immunodeficiency (LIM-56), Department of Dermatology, University of Sao Paulo Medical School, Sao Paulo, SP, Brazil.
authors.forename: A J
authors.initials: AJ
authors.lastname: da Silva Duarte
authors.affiliation: Laboratory of Dermatology and Immunodeficiency (LIM-56), Department of Dermatology, University of Sao Paulo Medical School, Sao Paulo, SP, Brazil.
authors.forename: M N
authors.initials: MN
authors.lastname: Sato
authors.affiliation: Laboratory of Dermatology and Immunodeficiency (LIM-56), Department of Dermatology, University of Sao Paulo Medical School, Sao Paulo, SP, Brazil.
authors.forename: V
authors.initials: V
authors.lastname: Aoki
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003876:Dermatitis, Atopic / Q000276:immunology*; D004804:Eosinophils / Q000378:metabolism*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D019041:L-Selectin / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D017455:Receptors, IgE / Q000276:immunology*; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*; D055815:Young Adult
chemical_list: D017455:Receptors, IgE; D019714:Tissue Inhibitor of Metalloproteinases; D019041:L-Selectin; D007073:Immunoglobulin E