title: Fabrication of Molecular Strain Probes for Illuminating Protein-Protein Interactions.
abstract: A unique bioluminescent imaging probe is introduced for illuminating molecular tension appended by protein-protein interactions (PPIs) of interest. A full-length luciferase is sandwiched between two proteins of interest via minimal flexible linkers. The ligand-activated PPIs append intramolecular tension to the sandwiched luciferase, boosting or dropping the enzymatic activity in a quantitative manner. This method guides construction of a new lineage of bioassays for ligand-activated PPIs.
authors.affiliation: Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki, 305-8569, Japan. kimu-sb@aist.go.jp.
authors.forename: Sung-Bae
authors.initials: SB
authors.lastname: Kim
authors.affiliation: Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki, 305-8569, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Fujii
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D008024:Ligands; D008156:Luciferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D049408:Luminescent Agents / Q000737:chemistry / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D025941:Protein Interaction Mapping / Q000379:methods*; D013329:Structure-Activity Relationship
chemical_list: D008024:Ligands; D049408:Luminescent Agents; D015335:Molecular Probes; D008156:Luciferases
keywords: Bioluminescence; Luciferase; Molecular tension; Protein–protein interactions