title: Synthetic Bioluminescent Coelenterazine Derivatives.
abstract: The development of coelenterazine (CTZ) derivatives resulting in superior optical characteristics is an efficient method to extend the range of its possible applications. Here, we describe the synthesis of three C-6 substituted CTZ derivatives retaining the recognition by Renilla luciferase (RLuc) and its derivatives. The novel derivatives are useful as bright blue-shifted CTZ derivatives, which can be used as an alternative to hitherto reported compound DeepBlueC™.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa, 223-8522, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Nishihara
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa, 223-8522, Japan.
authors.forename: Daniel
authors.initials: D
authors.lastname: Citterio
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa, 223-8522, Japan. suzuki@applc.keio.ac.jp.
authors.forename: Koji
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D007093:Imidazoles / Q000138:chemical synthesis* / Q000737:chemistry; D007700:Kinetics; D049410:Luciferases, Renilla / Q000235:genetics / Q000378:metabolism; D049408:Luminescent Agents / Q000138:chemical synthesis* / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods; D011719:Pyrazines / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D007093:Imidazoles; D049408:Luminescent Agents; D011719:Pyrazines; C017144:coelenterazine; D049410:Luciferases, Renilla
keywords: Bioluminescence; Chemiluminescence; Coelenterazine (CTZ); DeepBlueC; Luciferase; Luciferin; Renilla Luciferase (RLuc)