title: IL-17A-producing resident memory γδ T cells orchestrate the innate immune response to secondary oral Listeria monocytogenes infection.
abstract: Memory γδ T cells are important for the clearance of Listeria monocytogenes infection in the intestinal mucosa. However, the mechanisms by which memory γδ T cells provide protection against secondary oral infection are poorly understood. Here we used a recombinant strain of L. monocytogenes that efficiently invades the intestinal epithelium to show that Vγ4(+) memory γδ T cells represent a resident memory (Trm) population in the mesenteric lymph nodes (MLNs). The γδ Trm exhibited a remarkably static pattern of migration that radically changed following secondary oral L. monocytogenes infection. The γδ Trms produced IL-17A early after rechallenge and formed organized clusters with myeloid cells surrounding L. monocytogenes replication foci only after a secondary oral infection. Antibody blocking studies showed that in addition to IL-17A, the chemokine receptor C-X-C chemokine receptor 3 (CXCR3) is also important to enable the local redistribution of γδ Trm cells and myeloid cells specifically near the sites of L. monocytogenes replication within the MLN to restrict bacterial growth and spread. Our findings support a role for γδ Trms in orchestrating protective immune responses against intestinal pathogens.
authors.affiliation: Department of Immunology, UCONN Health, Farmington, CT 06030;
authors.forename: Pablo A
authors.initials: PA
authors.lastname: Romagnoli
authors.affiliation: Department of Immunology, UCONN Health, Farmington, CT 06030; Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794-5120;
authors.forename: Brian S
authors.initials: BS
authors.lastname: Sheridan
authors.affiliation: Department of Immunology, UCONN Health, Farmington, CT 06030;
authors.forename: Quynh-Mai
authors.initials: QM
authors.lastname: Pham
authors.affiliation: Department of Immunology, UCONN Health, Farmington, CT 06030;
authors.forename: Leo
authors.initials: L
authors.lastname: Lefrançois
authors.affiliation: Department of Immunology, UCONN Health, Farmington, CT 06030; Department of Pediatrics, UCONN Health, Farmington, CT 06030 kkhanna@uchc.edu.
authors.forename: Kamal M
authors.initials: KM
authors.lastname: Khanna
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000276:immunology; D005260:Female; D007113:Immunity, Innate / Q000276:immunology*; D007156:Immunologic Memory / Q000276:immunology; D020381:Interleukin-17 / Q000276:immunology* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D008089:Listeria monocytogenes / Q000276:immunology* / Q000502:physiology; D008088:Listeriosis / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D008198:Lymph Nodes / Q000276:immunology; D008643:Mesentery / Q000276:immunology; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D022423:Myeloid Cells / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D054367:Receptors, CXCR3 / Q000276:immunology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism
chemical_list: C515188:Cxcr3 protein, mouse; D020381:Interleukin-17; D016692:Receptors, Antigen, T-Cell, gamma-delta; D054367:Receptors, CXCR3
keywords: infections; intestinal immunity; resident memory cells; γδ T cells