title: Metabolic engineering of Yarrowia lipolytica to produce chemicals and fuels from xylose.
abstract: Yarrowia lipolytica is a biotechnological chassis for the production of a range of products, such as microbial oils and organic acids. However, it is unable to consume xylose, the major pentose in lignocellulosic hydrolysates, which are considered a preferred carbon source for bioprocesses due to their low cost, wide abundance and high sugar content. Here, we engineered Y. lipolytica to metabolize xylose to produce lipids or citric acid. The overexpression of xylose reductase and xylitol dehydrogenase from Scheffersomyces stipitis were necessary but not sufficient to permit growth. The additional overexpression of the endogenous xylulokinase enabled identical growth as the wild type strain in glucose. This mutant was able to produce up to 80g/L of citric acid from xylose. Transferring these modifications to a lipid-overproducing strain boosted the production of lipids from xylose. This is the first step towards a consolidated bioprocess to produce chemicals and fuels from lignocellulosic materials.
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France. Electronic address: rodrigo.ledesma@grignon.inra.fr.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Ledesma-Amaro
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France; Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Str. 37/41, 51-630 Wrocław, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Lazar
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Str. 37/41, 51-630 Wrocław, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rakicka
authors.affiliation: LISBP-Biocatalysis Group, INSA/INRA, Université de Toulouse, 135 Avenue de Rangueil, 31077 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, 31400 Toulouse, France; CNRS, UMR5504, 31400 Toulouse, France.
authors.forename: Zhongpeng
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
authors.forename: Florian
authors.initials: F
authors.lastname: Fouchard
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France. Electronic address: anne-marie.lecoq@jouy.inra.fr.
authors.forename: Anne-Marie Crutz-Le
authors.initials: AC
authors.lastname: Coq
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Nicaud
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D056804:Biofuels / Q000382:microbiology*; D053898:Biosynthetic Pathways / Q000235:genetics; D019343:Citric Acid / Q000302:isolation & purification / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods; D008055:Lipids / Q000096:biosynthesis*; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D014994:Xylose / Q000378:metabolism*; D025062:Yarrowia / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D056804:Biofuels; D008055:Lipids; D019343:Citric Acid; D014994:Xylose
keywords: Lignocellulosic materials; Metabolic engineering; Microbial oils; Synthetic biology; Xylose; Yarrowia lipolytica