title: Attomolar Zika virus oligonucleotide detection based on loop-mediated isothermal amplification and AC susceptometry.
abstract: Because of the serological cross-reactivity among the flaviviruses, molecular detection methods, such as reverse-transcription polymerase chain reaction (RT-PCR), play an important role in the recent Zika outbreak. However, due to the limited sensitivity, the detection window of RT-PCR for Zika viremia is only about one week after symptom onset. By combining loop-mediated isothermal amplification (LAMP) and AC susceptometry, we demonstrate a rapid and homogeneous detection system for the Zika virus oligonucleotide. Streptavidin-magnetic nanoparticles (streptavidin-MNPs) are premixed with LAMP reagents including the analyte and biotinylated primers, and their hydrodynamic volumes are dramatically increased after a successful LAMP reaction. Analyzed by a portable AC susceptometer, the changes of the hydrodynamic volume are probed as Brownian relaxation frequency shifts, which can be used to quantify the Zika virus oligonucleotide. The proposed detection system can recognize 1 aM synthetic Zika virus oligonucleotide in 20% serum with a total assay time of 27min, which can hopefully widen the detection window for Zika viremia and is therefore promising in worldwide Zika fever control.
authors.affiliation: Department of Engineering Sciences, Uppsala University, The Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
authors.forename: Bo
authors.initials: B
authors.lastname: Tian
authors.affiliation: Department of Engineering Sciences, Uppsala University, The Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Department of Immunology, Genetics and Pathology, Uppsala University, The Rudbeck Laboratory, SE-751 85 Uppsala, Sweden.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Engineering Sciences, Uppsala University, The Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
authors.forename: Teresa
authors.initials: T
authors.lastname: Zardán Gómez de la Torre
authors.affiliation: Acreo Swedish ICT AB, Arvid Hedvalls backe 4, SE-411 33 Göteborg, Sweden.
authors.forename: Christer
authors.initials: C
authors.lastname: Johansson
authors.affiliation: Department of Engineering Sciences, Uppsala University, The Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Svedlindh
authors.affiliation: Department of Engineering Sciences, Uppsala University, The Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden. Electronic address: mattias.stromberg@angstrom.uu.se.
authors.forename: Mattias
authors.initials: M
authors.lastname: Strömberg
mesh_terms: D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D004867:Equipment Design; D019544:Equipment Failure Analysis; D060306:Magnetometry / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry; D008832:Microchemistry / Q000295:instrumentation; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation*; D000071244:Zika Virus / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral
keywords: AC susceptometer; Brownian relaxation; Loop-mediated isothermal amplification; Magnetic nanoparticles; Zika virus