title: Antibody-based detection of advanced glycation end-products: promises vs. limitations.
abstract: Advanced glycation end-products (AGEs) of the Maillard reaction were originally measured according to their fluorescent and browning properties. A subsequent study with instrumental analyses such as high-performance liquid chromatography and gas chromatography mass spectrometry more clearly demonstrated the involvement of each AGE structure in pathological conditions. Furthermore, immunochemical methods have also been developed to clarify the localization of AGEs in tissues and measurement of AGEs in multiple clinical samples. Although the involvement of AGEs in age-related diseases has progressed due to immunochemical techniques, the relationship between AGE structure and diseases has not been clear because little was known about the epitope structure of each anti-AGE antibody. However, the development of epitope-identified antibodies against AGEs has made it possible to clarify AGE structures involved in diseases. This review discusses not only the usability of anti-AGE antibodies to evaluate AGEs and disease pathology and screen AGE inhibitors, but also describes their usage.
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto, 869-1404, Japan. nagai-883@umin.ac.jp.
authors.forename: Ryoji
authors.identifier: 0000-0003-0763-0067
authors.initials: R
authors.lastname: Nagai
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto, 869-1404, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Shirakawa
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto, 869-1404, Japan.
authors.forename: Rei-Ichi
authors.initials: R
authors.lastname: Ohno
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto, 869-1404, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Hatano
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto, 869-1404, Japan.
authors.forename: Hikari
authors.initials: H
authors.lastname: Sugawa
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto, 869-1404, Japan.|Department of Orthopaedic Surgery, Jikei University School of Medicine, Minato, Tokyo, Japan.
authors.forename: Shoutaro
authors.initials: S
authors.lastname: Arakawa
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto, 869-1404, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Ichimaru
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto, 869-1404, Japan.
authors.forename: Shoh
authors.initials: S
authors.lastname: Kinoshita
authors.affiliation: Department of Pathology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sakata
authors.affiliation: Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto, 869-1404, Japan.
authors.forename: Mime
authors.initials: M
authors.lastname: Nagai
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods
chemical_list: D000906:Antibodies; D017127:Glycation End Products, Advanced
keywords: AGEs; Anti-AGEs antibody; Atherosclerosis; CML; Diabetes; Lifestyle-related diseases