title: Quantum Yield Determination Based on Photon Number Measurement, Protocols for Firefly Bioluminescence Reactions.
abstract: Quantum yield (QY), which is defined as the probability of photon production by a single bio/chemiluminescence reaction, is an important factor to characterize luminescence light intensity emitted diffusively from the reaction solution mixture. Here, methods to measure number of photons to determine QY according to the techniques of national radiometry standards are described. As an example, experiments using firefly bioluminescence reactions are introduced.
authors.affiliation: Quantum Optical Measurement Group, National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, 1-1-1 Umezono, Tsukuba, Ibaraki, 563-8563, Japan. niwa-k@aist.go.jp.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Niwa
mesh_terms: D049409:Luciferases, Firefly / Q000378:metabolism*; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods; D017785:Photons; D011789:Quantum Theory
chemical_list: D049409:Luciferases, Firefly
keywords: Bioluminescence; Firefly luciferase; Luminometer; National radiometry standard; Quantum yield