title: Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)-Based Protein Profiling in Plants.
abstract: Isobaric tags for relative and absolute quantitation (iTRAQ) is a technology that utilizes isobaric reagents to label the primary amines of peptides and proteins and is used in proteomics to study quantitative changes in the proteome by tandem mass spectrometry . Here, we present an adaptation of the iTRAQ experimental protocol for plants that allows the identification and quantitation of more than 12,000 plant proteins in Arabidopsis with a false discovery rate of less than 5 %.
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Isabel Cristina
authors.initials: IC
authors.lastname: Vélez-Bermúdez
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Tuan-Nan
authors.initials: TN
authors.lastname: Wen
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.|Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Lan
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. wosh@gate.sinica.edu.tw.|Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China. wosh@gate.sinica.edu.tw.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schmidt
mesh_terms: D002853:Chromatography, Liquid; D010455:Peptides / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D020543:Proteome / Q000145:classification / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000737:chemistry
chemical_list: D010455:Peptides; D010940:Plant Proteins; D020543:Proteome; D014357:Trypsin
keywords: Absolute quantitation; Isobaric tags; Mass spectrometry; Primary amines; Proteomics