title: In vitro Quality of Platelets with Low Plasma Carryover Treated with Ultraviolet C Light for Pathogen Inactivation.
abstract: BACKGROUND
  The THERAFLEX UV-Platelets system uses shortwave ultraviolet C light (UVC, 254 nm) to inactivate pathogens in platelet components. Plasma carryover influences pathogen inactivation and platelet quality following treatment. The plasma carryover in the standard platelets produced by our institution are below the intended specification (<30%).
  
  
  METHODS
  A pool and split study was carried out comparing untreated and UVC-treated platelets with <30% plasma carryover (n = 10 pairs). This data was compared to components that met specifications (>30% plasma). The platelets were tested over storage for in vitro quality.
  
  
  RESULTS
  Platelet metabolism was accelerated following UVC treatment, as demonstrated by increased glucose consumption and lactate production. UVC treatment caused increased externalization of phosphatidylserine on platelets and microparticles, activation of the GPIIb/IIIa receptor (PAC-1 binding), and reduced hypotonic shock response. Platelet function, as measured with thrombelastogram, was not affected by UVC treatment. Components with <30% plasma were similar to those meeting specification with the exception of enhanced glycolytic metabolism.
  
  
  CONCLUSIONS
  This in vitro analysis demonstrates that treatment of platelets with <30% plasma carryover with the THERAFLEX UV-Platelets system affects some aspects of platelet metabolism and activation, although in vitro platelet function was not negatively impacted. This study also provides evidence that the treatment specifications of plasma carryover could be extended to below 30%.
authors.affiliation: Research and Development, Australian Red Cross Blood Service, Sydney, NSW, Australia.
authors.forename: Lacey
authors.initials: L
authors.lastname: Johnson
authors.affiliation: Research and Development, Australian Red Cross Blood Service, Sydney, NSW, Australia.
authors.forename: Ryan
authors.initials: R
authors.lastname: Hyland
authors.affiliation: Research and Development, Australian Red Cross Blood Service, Sydney, NSW, Australia.
authors.forename: Shereen
authors.initials: S
authors.lastname: Tan
authors.affiliation: MacoPharma International GmbH, Langen, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Tolksdorf
authors.affiliation: MacoPharma, Tourcoing, France.
authors.forename: Chryslain
authors.initials: C
authors.lastname: Sumian
authors.affiliation: German Red Cross Blood Service NSTOB, Springe, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Seltsam
authors.affiliation: Research and Development, Australian Red Cross Blood Service, Sydney, NSW, Australia.
authors.forename: Denese
authors.initials: D
authors.lastname: Marks
keywords: Pathogen inactivation; Plasma carryover; Platelet concentrates; UVC