title: Sesquiterpene Coumarins from Ferula sinkiangensis Act as Neuroinflammation Inhibitors.
abstract: Neuroinflammation mediated by microglia cells plays a critical role in the development of Alzheimer's disease. To identify novel natural neuroinflammation inhibitors, a bioactivity-guided phytochemical research was performed on the traditional Chinese medicine "Awei", that exhibited a significant inhibitory effect on nitric oxide production in over-activated microglia cells. The research identified sixteen bioactive sesquiterpene coumarins (two new and fourteen known ones) in the effective extract ofFerula sinkiangensis. Further, the anti-neuroinflammatory activities in BV-2 microglial cells were evaluated by monitoring LPS-induced nitric oxide production. In conclusion, the major constituent, (3'S, 5'S, 8'R, 9'S, 10'R)-kellerin (1.5 %, w/w), should be responsible for the anti-neuroinflammatory effect exhibited by Awei. Furthermore, it might be a potential natural therapeutic agent for Alzheimer's disease. The research indicated moreover, that its primary mechanism is the inhibition of mRNA expression of the inflammatory cytokines nitric oxide, tumor necrosis factor-α, cyclooxygenase-2, interleukin-6 and interleukin-1β.
authors.affiliation: School of Traditional Chinese Materia Medica 49﹟, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Yachao
authors.initials: Y
authors.lastname: Xing
authors.affiliation: School of Traditional Chinese Materia Medica 49﹟, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: School of Traditional Chinese Materia Medica 49﹟, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Di
authors.initials: D
authors.lastname: Zhou
authors.affiliation: School of Traditional Chinese Materia Medica 49﹟, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: College of Life and Health Sciences, Northeastern University, Shenyang, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Jiao
authors.affiliation: School of Traditional Chinese Materia Medica 49﹟, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Traditional Chinese Materia Medica 49﹟, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Si
authors.affiliation: College of Life and Health Sciences, Northeastern University, Shenyang, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Hou
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D005309:Ferula / Q000737:chemistry*; D007249:Inflammation / Q000188:drug therapy; D008070:Lipopolysaccharides / Q000009:adverse effects; D051379:Mice; D017628:Microglia / Q000187:drug effects; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012116:Resins, Plant / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D003374:Coumarins; D016207:Cytokines; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D012333:RNA, Messenger; D012116:Resins, Plant; D012717:Sesquiterpenes; D009569:Nitric Oxide