title: Preventive Effects of Houttuynia cordata Extract for Oral Infectious Diseases.
abstract: Houttuynia cordata (HC) (Saururaceae) has been used internally and externally as a traditional medicine and as an herbal tea for healthcare in Japan. Our recent survey showed that HC poultice (HCP) prepared from smothering fresh leaves of HC had been frequently used for the treatment of purulent skin diseases with high effectiveness. Our experimental study also demonstrated that ethanol extract of HCP (eHCP) has antibacterial, antibiofilm, and anti-inflammatory effects against S. aureus which caused purulent skin diseases. In this study, we focused on novel effects of HCP against oral infectious diseases, such as periodontal disease and dental caries. We determined the antimicrobial and antibiofilm effects of water solution of HCP ethanol extract (wHCP) against important oral pathogens and investigated its cytotoxicity and anti-inflammatory effects on human oral epithelial cells. wHCP had moderate antimicrobial effects against some oral microorganisms and profound antibiofilm effects against Fusobacterium nucleatum, Streptococcus mutans, and Candida albicans. In addition, wHCP had no cytotoxic effects and could inhibit interleukin-8 and CCL20 productions by Porphyromonas gingivalis lipopolysaccharide-stimulated human oral keratinocytes. Our findings suggested that wHCP may be clinically useful for preventing oral infectious diseases as a mouthwash for oral care.
authors.affiliation: Department of Pharmacognosy, Institute of Biomedical Sciences, Tokushima University Graduate School, 1-78-1 Shou-machi, Tokushima 770-8505, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Sekita
authors.affiliation: Department of Oral Microbiology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Murakami
authors.affiliation: Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.
authors.forename: Hiromichi
authors.identifier: 0000-0002-8659-9776
authors.initials: H
authors.lastname: Yumoto
authors.affiliation: Department of Oral Microbiology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Amoh
authors.affiliation: Department of Oral Microbiology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.
authors.forename: Natsumi
authors.identifier: 0000-0002-9020-9478
authors.initials: N
authors.lastname: Fujiwara
authors.affiliation: Department of Oral Microbiology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Ogata
authors.affiliation: Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuo
authors.affiliation: Department of Oral Microbiology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Miyake
authors.affiliation: Department of Pharmacognosy, Institute of Biomedical Sciences, Tokushima University Graduate School, 1-78-1 Shou-machi, Tokushima 770-8505, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Kashiwada
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D001419:Bacteria / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003731:Dental Caries / Q000382:microbiology*; D052859:Houttuynia / Q000737:chemistry*; D006801:Humans; D010510:Periodontal Diseases / Q000382:microbiology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts