title: Analyzing the CDR3 Repertoire with respect to TCR-Beta Chain V-D-J and V-J Rearrangements in Peripheral T Cells using HTS.
abstract: V-D-J rearrangement of the TCR-beta chain follows the 12/23 rule and the beyond 12/23 restriction. Currently, the proportion and characteristics of TCR-beta chain V-J rearrangement is unclear. We used high-throughput sequencing to compare and analyze TCR-beta chain V-J rearrangement and V-D-J rearrangement in the CDR3 repertoires of T cells from the PBMCs of six volunteers and six BALB/c mice. The results showed that the percentage of V-J rearrangement of the volunteers was approximately 0.7%, whereas that of the mice was 2.2%. The clonality of mice V-J rearrangement was significantly reduced compared with the V-D-J rearrangement, whereas the clonality of human V-J rearrangement was slightly reduced compared with the V-D-J rearrangement. V-J rearrangement in CDR3 involved the significant usage of N, S, F and L, whereas V-D-J rearrangement in CDR3 involved the significant usage of R and G. The levels of V deletion and J deletion in V-J rearrangement were significantly reduced compared with V-D-J rearrangement. TRBD and TRBJ usage in V-J rearrangement differed from that of V-D-J rearrangement, including dominant usage of TRBV and TRBJ and their pairing. Taken together, these results provide new ideas and technology for studies of V-D-J rearrangement and V-J rearrangement in the CDR3 repertoire.
authors.affiliation: Department of Immunology, Research Center for Medicine &Biology, Innovation &Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563003, China.
authors.forename: Long
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Immunology, Research Center for Medicine &Biology, Innovation &Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563003, China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, Zunyi Medical University, Zunyi 563003, China.
authors.lastname: Bin Shi
authors.affiliation: Department of Immunology, Research Center for Medicine &Biology, Innovation &Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563003, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Immunology, Research Center for Medicine &Biology, Innovation &Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563003, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Peng
authors.affiliation: Department of Immunology, Research Center for Medicine &Biology, Innovation &Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563003, China.
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Immunology, Research Center for Medicine &Biology, Innovation &Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563003, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Breast Surgery, The first Affiliated Hospital of Zunyi Medical University, Zunyi 563003, China.
authors.forename: Suhong
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Immunology, Research Center for Medicine &Biology, Innovation &Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563003, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of Immunology, Research Center for Medicine &Biology, Innovation &Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563003, China.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Yao
mesh_terms: D000328:Adult; D000818:Animals; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013601:T-Lymphocytes / Q000378:metabolism*; D055815:Young Adult
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta