title: [Downregulation of miR-18a or miR-328 inhibits the invasion and migration of lung adenocarcinoma A549 cells].
abstract: Objective To investigate the expressions of miR-18a and miR-328 in lung adenocarcinoma A549 cells, and explore the effect of miR-18a or miR-328 on invasion and migration of A549 cells. Methods The expressions of miR-18a and miR-328 in A549 cells were detected by real-time quantitative PCR. Then the specific miR-18a or miR-328 inhibitor sequences were transfected into A549 cells to downregulate the expression of miR-18a or miR-328. The invasion and migration abilities of A549 cells were evaluated by Transwell(TM) assay. Results The miR-18a and miR-328 were overexpressed in A549 cells. And with the corresponding inhibitors being transfected, the expressions of miR-18a and miR-328 in A549 cells were downregulated. In addition, TranswellTM assay showed that decreased expression of miR-18a or miR-328 significantly inhibited the invasion and migration of A549 cells. Conclusion Downregulation of miR-18a or miR-328 can inhibit the invasion and migration abilities of A549 cells effectively.
authors.affiliation: Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Chuanchong
authors.initials: C
authors.lastname: DU
authors.affiliation: Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China. *Corresponding author, E-mail: jxuzh135@163.com.
authors.forename: Jinxu
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Lu
authors.affiliation: Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D002460:Cell Line; D054443:Cell Migration Assays / Q000295:instrumentation / Q000379:methods; D002465:Cell Movement / Q000235:genetics*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C522576:MIRN18A microRNA, human; C548170:MIRN328 microRNA, human; D035683:MicroRNAs