title: Traditional herbal prescription LASAP-C inhibits melanin synthesis in B16F10 melanoma cells and zebrafish.
abstract: BACKGROUND
  In this study, the anti-melanogenesis efficacy of clinically used herbal prescription LASAP-C, which consists of four herbal medicines-Rehmanniae Radix Crudus, Lycii Fructus, Scutellariae Radix, and Angelicae Dahuricae Radix, was investigated.
  
  
  METHODS
  The chemical profile of LASAP-C was established by conducting ultra-performance liquid chromatography-electrospray ionization-mass spectrometry. Anti-melanogenic efficacy was evaluated by tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 expression in B16F10 melanoma cells. In vivo evaluation was performed by using zebrafish model.
  
  
  RESULTS
  Molecular evidences suggested that melanin synthesis was inhibited via the down-regulation of tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 expression in B16F10 melanoma cells treated with LASAP-C. The anti-melanogenesis efficacy was also confirmed in vivo by using the zebrafish model.
  
  
  CONCLUSIONS
  The results of this study provide strong evidences that LASAP-C can be used as an active component in cosmeceutical products for reducing excess pigmentation in the human skin.
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.|Division of Pharmacognosy, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea.
authors.forename: Chae Young
authors.initials: CY
authors.lastname: Bang
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University, 266 Munhwa-ro, Jung-gu, Daejeon, 301-747, South Korea.
authors.forename: Mi Yoon
authors.initials: MY
authors.lastname: Kim
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University, 266 Munhwa-ro, Jung-gu, Daejeon, 301-747, South Korea.
authors.forename: Jeung-Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, 88 Daekak-ro, Yuseong-gu, Daejeon, 305-764, South Korea.
authors.forename: Hyunju
authors.initials: H
authors.lastname: Ro
authors.affiliation: Dongguk University Bundang Oriental Hospital, 268 Buljeong-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, South Korea.
authors.forename: Min-Sun
authors.initials: MS
authors.lastname: Choi
authors.affiliation: Dongguk University International Hospital, 27 Donkguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, South Korea.
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Kim
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea. ypjang@khu.ac.kr.|Division of Pharmacognosy, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea. ypjang@khu.ac.kr.
authors.forename: Young Pyo
authors.identifier: http://orcid.org/0000-0001-5865-9228
authors.initials: YP
authors.lastname: Jang
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea. sychoung@khu.ac.kr.|Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, South Korea. sychoung@khu.ac.kr.
authors.forename: Se Young
authors.initials: SY
authors.lastname: Choung
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D019746:Intramolecular Oxidoreductases / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D004364:Pharmaceutical Preparations; D008517:Phytotherapy; D010858:Pigmentation / Q000187:drug effects; D010936:Plant Extracts / Q000627:therapeutic use*; D015027:Zebrafish
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C000611874:LASAP-C; D008543:Melanins; D008562:Membrane Glycoproteins; D004364:Pharmaceutical Preparations; D010936:Plant Extracts; D010088:Oxidoreductases; C079621:Tyrp1 protein, mouse; D014442:Monophenol Monooxygenase; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase
keywords: Herbal prescription; LASAP-C; Melanin; TRP-1; TRP-2; Tyrosinase; Zebrafish