title: Effects of Different Concentrations of Collagenous Peptide from Fish Scales on Osteoblast Proliferation and Osteoclast Resorption.
abstract: The incidence of osteoporosis has increased among the elderly population. Establishing a model of bone remodeling for screening new drugs is critical to identify safe and effective treatments for osteoporosis. In this study, we established a platform to investigate the therapeutic effects of collagenous peptides extracted from scales of two kinds of fish, namely, sparidae and chanos. These peptides were prepared using seven concentrations of collagenous peptide: 100, 80, 60, 40, 20, 10 and 1 mg/ml. Experimental results indicated that collagenous peptides promoted the proliferation of osteoblasts and inhibited the proliferation of mature osteoclasts; the effective concentration of collagenous peptide-sparidae was 10 mg/ml and that of collagenous peptide-chanos was 40 mg/ml. These findings demonstrate that, to a certain extent, collagenous peptides extracted from fish scales can be used to prevent osteoporosis to assist bone remodeling.
authors.affiliation: Institute of Dermatology and Department of Dermatology, No.1 Hospital, Anhui Medical University, Hefei 230032, Anhui, People's Republic of China.
authors.forename: Chung-Hsuan
authors.initials: CH
authors.lastname: Hu
authors.affiliation: Biomaterials Translational Research Center, China Medical University Hospital, Taichung 40202, Taiwan, R.O.C.|Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung 40202, Taiwan, R.O.C.|School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan, R.O.C.|Department of Biomedical Informatics, Asia University, Taichung 41354, Taiwan, R.O.C.
authors.forename: Chun-Hsu
authors.initials: CH
authors.lastname: Yao
authors.affiliation: Department of Medical Education and Research, China Medical University Beigan Hospital, Yunlin 65152, Taiwan, R.O.C.
authors.forename: Tzu-Min
authors.initials: TM
authors.lastname: Chan
authors.affiliation: Department of Medical Education and Research, China Medical University Beigan Hospital, Yunlin 65152, Taiwan, R.O.C.
authors.forename: Teng-Le
authors.initials: TL
authors.lastname: Huang
authors.affiliation: Institute of Dermatology and Department of Dermatology, No.1 Hospital, Anhui Medical University, Hefei 230032, Anhui, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sen
authors.affiliation: Biomaterials Translational Research Center, China Medical University Hospital, Taichung 40202, Taiwan, R.O.C.|Department of Biotechnology, Asia University, Taichung 41354, Taiwan, R.O.C.|Graduate Institute of Basic Medical Science, China Medical University, Taichung 40202, Taiwan, R.O.C.
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Biomaterials Translational Research Center, China Medical University Hospital, Taichung 40202, Taiwan, R.O.C.|Department of Family medicine, China Medical University hospital, Taichung 40202, Taiwan, Republic of China.
authors.forename: Chien-Yi
authors.initials: CY
authors.lastname: Ho
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000517:prevention & control; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D029941:Fish Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects*; D010010:Osteoclasts / Q000187:drug effects*; D010024:Osteoporosis / Q000517:prevention & control*; D010473:Perciformes
chemical_list: D029941:Fish Proteins; D003094:Collagen