title: ROS-mediated apoptosis of HAPI microglia through p53 signaling following PFOS exposure.
abstract: Perfluorooctane sulfonate (PFOS), the most extensively studied member of perfluoroalkyl and polyfluoroalkyl substances (PFASs), has been thought to be toxic to the central nervous system (CNS) of mammals. However, the neurotoxic effects of PFOS remain largely unknown. In this study, the effect of PFOS on microglial apoptosis was examined. The results showed that PFOS could significantly reduce the cell viability and mediate cell apoptosis in HAPI microglia, which was closely accompanied with ROS production and p53 overexpression. Moreover, p53 interference significantly ameliorated PFOS-triggered cytotoxic effects in HAPI microglia, including the downregulation of cleaved PARP and cleaved caspase 3. Interestingly, NAC, a ROS inhibitor, inhibited p53 expression, and decreased the apoptosis of HAPI microglia. Taken together, these findings suggest that upregulated production of ROS plays a vital role in PFOS-mediated apoptosis in HAPI microglia via the modulation of p53 signaling.
authors.affiliation: Physiatry Department, The Second People's Hospital of Nan Tong, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Ge
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Nie
authors.affiliation: Department of Public Health, The Second People's Hospital of Nan Tong, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Yang
authors.affiliation: Physiatry Department, The Second People's Hospital of Nan Tong, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Hongjian
authors.initials: H
authors.lastname: Lu
authors.affiliation: Physiatry Department, The Second People's Hospital of Nan Tong, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Xinjian
authors.initials: X
authors.lastname: Song
authors.affiliation: School of Life Science, University of Science and Technology of China, Hefei, Anhui 230000, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Su
authors.affiliation: Department of Labor and Environmental Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Labor and Environmental Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Jingling
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Labor and Environmental Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Jiamin
authors.initials: J
authors.lastname: Mao
authors.affiliation: Department of Labor and Environmental Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Jianya
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Labor and Environmental Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226001, People's Republic of China.
authors.forename: Shengyang
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226001, People's Republic of China. Electronic address: wqy@ntu.edu.cn.
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Wu
mesh_terms: D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity; D004789:Enzyme Activation / Q000187:drug effects; D005466:Fluorocarbons / Q000633:toxicity*; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D017738:Alkanesulfonic Acids; D004785:Environmental Pollutants; D005466:Fluorocarbons; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; C076994:perfluorooctane sulfonic acid; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3
keywords: Microglia; PFOS; ROS; apoptosis; p53