title: The CREB3-Herp signalling module limits the cytosolic calcium concentration increase and apoptosis induced by poliovirus.
abstract: Poliovirus (PV)-induced apoptosis seems to play a major role in central nervous system (CNS) tissue injury, a crucial feature of the pathogenesis of poliomyelitis. We have previously shown that calcium (Ca2+) flux from the endoplasmic reticulum (ER) to the cytosol during PV infection is involved in apoptosis induction in human neuroblastoma cells. We show here that PV infection is associated with a transient upregulation of Herp (homocysteine-induced ER protein), a protein known to promote the degradation of ER-resident Ca2+ channels. Herp gene transcription is controlled by the transcription factor CREB3 (cAMP response element-binding protein 3). We found that the CREB3/Herp pathway limited the increase in cytosolic Ca2+ concentration and apoptosis early in PV infection. This may reduce the extent of PV-induced damage to the CNS during poliomyelitis.
authors.affiliation: Institut Pasteur, Unité de Biologie des virus entériques, 25 rue du Dr Roux, 75015 Paris, France.|INSERM U994, Paris, France.
authors.forename: Carmen
authors.initials: C
authors.lastname: Mirabelli
authors.affiliation: Institut Pasteur, Unité de Biologie des virus entériques, 25 rue du Dr Roux, 75015 Paris, France.|INSERM U994, Paris, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Pelletier
authors.affiliation: Institut Pasteur, Unité de Biologie des virus entériques, 25 rue du Dr Roux, 75015 Paris, France.|INSERM U994, Paris, France.|Université Versailles Saint-Quentin, Versailles, France.
authors.forename: François
authors.initials: F
authors.lastname: Téoulé
authors.affiliation: Institut Pasteur, Unité de Génomique virale et vaccination, 25 rue du Dr Roux, 75015 Paris, France.|CNRS UMR 3569, Paris, France.
authors.forename: Pierre-Olivier
authors.initials: PO
authors.lastname: Vidalain
authors.affiliation: Institut Pasteur, Unité de Biologie des virus entériques, 25 rue du Dr Roux, 75015 Paris, France.|Université Versailles Saint-Quentin, Versailles, France.|INSERM U994, Paris, France.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Brisac
authors.affiliation: Institut Pasteur, Unité de Génomique virale et vaccination, 25 rue du Dr Roux, 75015 Paris, France.|CNRS UMR 3569, Paris, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Tangy
authors.affiliation: Institut Pasteur, Unité de Biologie des virus entériques, 25 rue du Dr Roux, 75015 Paris, France.|INSERM U994, Paris, France.
authors.forename: Francis
authors.initials: F
authors.lastname: Delpeyroux
authors.affiliation: Institut Pasteur, Unité de Biologie des virus entériques, 25 rue du Dr Roux, 75015 Paris, France.|INSERM U994, Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Blondel
mesh_terms: D017209:Apoptosis; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D009474:Neurons / Q000276:immunology / Q000378:metabolism / Q000821:virology; D017955:Poliovirus / Q000276:immunology* / Q000472:pathogenicity*; D015398:Signal Transduction
chemical_list: C107941:CREB3 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; C485932:HERPUD1 protein, human; D008565:Membrane Proteins; D002118:Calcium