title: Overexpression of GhSARP1 encoding a E3 ligase from cotton reduce the tolerance to salt in transgenic Arabidopsis.
abstract: Ubiquitination plays a very important role in the response to abiotic stresses of plant. To identify key regulators of salt stress, a gene GhSARP1(Salt-Associated Ring finger Protein)encoding C3H2C3-type E3 ligase, was cloned from cotton. Transcription level of GhSARP1 was high in leaf, flower and fiber of 24,27 and 27DPA (Days Post-Anthesis), but low in root and stem. Except PEG6000 treatment, the expression of GhSARP1 was down-regulated by NaCl, cold and ABA after being treated for 1 h. GhSARP1-GFP fusion protein located on the plasma membrane, which was dependent on trans-membrane motif. In vitro ubiquitination assay showed that GhSARP1 had E3 ligase activity. Heterogeneous overexpression of GhSARP1reduced salt tolerance of transgenic Arabidopsis in germination and post-germination stage. Our results suggested that the GhSARP1 might negatively regulate the response to salt stress mediated by the ubiquitination in cotton.
authors.affiliation: College of Agriculture, The Key Laboratory of Oasis Eco-Agriculture, Shihezi University, Shihezi 832000, Xinjiang Province, China.
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Agriculture, The Key Laboratory of Oasis Eco-Agriculture, Shihezi University, Shihezi 832000, Xinjiang Province, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Agriculture, The Key Laboratory of Oasis Eco-Agriculture, Shihezi University, Shihezi 832000, Xinjiang Province, China.
authors.forename: Shouhong
authors.initials: S
authors.lastname: Zhu
authors.affiliation: College of Agriculture, The Key Laboratory of Oasis Eco-Agriculture, Shihezi University, Shihezi 832000, Xinjiang Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Agriculture, The Key Laboratory of Oasis Eco-Agriculture, Shihezi University, Shihezi 832000, Xinjiang Province, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Agriculture, The Key Laboratory of Oasis Eco-Agriculture, Shihezi University, Shihezi 832000, Xinjiang Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Agriculture, The Key Laboratory of Oasis Eco-Agriculture, Shihezi University, Shihezi 832000, Xinjiang Province, China. Electronic address: sunjiezh@vip.sina.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D003368:Gossypium / Q000201:enzymology*; D009928:Organ Specificity / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified; D021381:Protein Transport / Q000187:drug effects; D055049:Salt Tolerance / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D012965:Sodium Chloride; D044767:Ubiquitin-Protein Ligases
keywords: E3 ligase; Gossypium hirsutum; RING finger protein; Salt stress