title: Interactomic landscape of PA-X-chicken protein complexes of H5N1 influenza A virus.
abstract: As a newly identified isoform generated from the PA segment and an essential factor for viral virulence, little is known about PA-X-host interactions. Here we present the interactomic landscape of PA-X protein of H5N1 influenza A virus (IAV), described using data generated from affinity purification and mass spectrometry (AP-MS). PA-X was exogenously expressed in chicken fibroblast cells and PA-X associated protein complexes were identified by AP-MS. Using a high confidence threshold for interaction 56 unique proteins were found to have physical interactions with PA-X. PA-X associated host factors showed strong enrichment for specific protein domains, including annexin and WD40 domains. Many proteins that have been described as pro or antiviral host proteins interact with PA-X, indicating the possible effect of these proteins on influenza A viral infections facilitated by interactions with PA-X. This study has uncovered the comprehensive interactomic landscape of PA-X and laid the foundation for further understanding of PA-X function in terms of viral-host protein interactions.
  
  
  
  Identification of viral-host interacting proteins is vital for the comprehensive understanding of how virus recruits the host cellular machinery and how host antagonizes virus infection. Our study reveals the viral-host interactome of PA-X and uncovers interactions between host proteins and PA-X which might have crucial roles in viral infection.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, P, .R. China.
authors.forename: Qinghe
authors.initials: Q
authors.lastname: Li
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, P, .R. China; College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 2250000, PR China.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, P, .R. China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 2250000, PR China.
authors.forename: Guobin
authors.initials: G
authors.lastname: Chang
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, P, .R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, P, .R. China.
authors.forename: Ranran
authors.initials: R
authors.lastname: Liu
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, P, .R. China.
authors.forename: Maiqing
authors.initials: M
authors.lastname: Zheng
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 2250000, PR China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Chen
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, P, .R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wen
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, P, .R. China. Electronic address: zhaoguiping@caas.net.cn.
authors.forename: Guiping
authors.initials: G
authors.lastname: Zhao
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002645:Chickens / Q000821:virology*; D002846:Chromatography, Affinity; D054884:Host-Pathogen Interactions; D053124:Influenza A Virus, H5N1 Subtype / Q000737:chemistry*; D005585:Influenza in Birds; D013058:Mass Spectrometry; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: D030161:Avian Proteins; C573926:PA-X protein, influenza A virus; D012097:Repressor Proteins; D017361:Viral Nonstructural Proteins
keywords: Chicken; H5N1 avian influenza A virus; Interactome; PA-X