title: Differential protein expression in the secretory fluids of maxillary sinusitis and maxillary retention cyst.
abstract: Both maxillary sinusitis (MS) and maxillary retention cyst (MRC) involve the maxillary sinus and show similar clinical features. Clinically, differentiating between MS and MRC is sometimes difficult in asymptomatic patients, despite their quite different pathogenic behaviors. To identify differential protein expressions in the secretory fluids of MS and MRC, 25 cases of asymptomatic MS and 15 cases of asymptomatic MRC were examined pathologically in this study. All patients underwent routine endoscopic sinus surgery or modified Caldwell-Luc procedure and the sinus mucosal specimens obtained during these procedures with the approval of the Institutional Review Board. Their secretory fluids were analyzed via immunoprecipitation-based high-performance liquid chromatography (IP-HPLC) using 25 types of antiserum, including inflammatory cytokines, antimicrobial proteins, and mucosal protective proteins. In the histological examinations, MS and MRC showed similar features in the secretory columnar epithelial lining and thick submucosal connective tissue, both of which contained few inflammatory cells infiltrates. The IP-HPLC analysis revealed that TNFα, IL-1, -8, MMP-3, -10, α1-antitrypsin, cathepsin C, lysozyme, lactoferrin, β-defensin-1, -3, LL-37, mucocidin, and mucin-1 were more intensely expressed in MS than in MRC; whereas IgA, cystatin A, and proline-rich proteins were more strongly expressed in MRC than in MS. These data indicate that the secretory fluid of MS is indicative of a more robust inflammatory reaction to certain bacteria compared to that of MRC, while the secretory fluid of MRC contains more abundant mucosal protective proteins compared to that of MS. Taken together, the IP-HPLC analysis of MS and MRC secretory fluid revealed that MRC showed a weaker inflammatory reaction but a stronger mucosal protective function than MS.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Research Institute, Seoul National University, Seoul, Korea.
authors.forename: Soung Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Research Institute, Seoul National University, Seoul, Korea.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Eo
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Research Institute, Seoul National University, Seoul, Korea.
authors.forename: Yun Ju
authors.initials: YJ
authors.lastname: Cho
authors.affiliation: Department of Dental Hygiene, Cheongju University, Cheongju, Korea.
authors.forename: Yeon Sook
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Oral Pathology, College of Dentistry, Gangneung-Wonju National University, 123 Chibyun-dong, Gangneung, 210-702, Korea. sukkeunlee@hanmail.net.
authors.forename: Suk Keun
authors.initials: SK
authors.lastname: Lee
mesh_terms: D000328:Adult; D058070:Asymptomatic Diseases; D015415:Biomarkers / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002908:Chronic Disease; D003560:Cysts / Q000175:diagnosis* / Q000378:metabolism; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008443:Maxillary Sinus / Q000378:metabolism*; D015523:Maxillary Sinusitis / Q000175:diagnosis* / Q000378:metabolism; D008875:Middle Aged; D020545:Respiratory Mucosa / Q000378:metabolism*
chemical_list: D015415:Biomarkers
keywords: IP-HPLC; Maxillary retention cyst (MRC); Maxillary sinusitis (MS); Protein expression