title: Effect of ozonolysis parameters on the inhibitory compound generation and on the production of ethanol by Pichia stipitis and acetone-butanol-ethanol by Clostridium from ozonated and water washed sugarcane bagasse.
abstract: Sugarcane bagasse (SCB) was ozone pretreated and detoxified by water washing, applying a L9(3)(4) orthogonal array (OA) design of experiments to study the effect of pretreatment parameters (moisture content, ozone concentration, ozone/oxygen flow and particle size) on the generation of inhibitory compounds and on the composition of hydrolysates of ozonated-washed samples. Ozone concentration resulted the highest influence process parameter on delignification and sugar release after washing; while, for inhibitory compound formation, moisture content also had an important role. Ozone expended in pretreatment related directly with sugar release and inhibitory compound formation. Washing detoxification was effective, providing non-inhibitory hydrolysates. Maximum glucose and xylose release yields obtained were 84% and 67%, respectively, for ozonated-washed SCB. Sugar concentration resulted in the decisive factor for biofuels yields. Ethanol production achieved an 88% yield by Pichia stipitis, whereas Clostridium acetobutylicum produced 0.072gBUTANOL/gSUGAR and 0.188gABE/gSUGAR, and, Clostridium beijerinckii 0.165gBUTANOL/gSUGAR and 0.257gABE/gSUGAR.
authors.affiliation: Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid - UVa, Calle Doctor Mergelina, s/n, 47005 Valladolid, Spain.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Travaini
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid - UVa, Paseo de Belén, 7, 47011 Valladolid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Barrado
authors.affiliation: Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid - UVa, Calle Doctor Mergelina, s/n, 47005 Valladolid, Spain. Electronic address: silvia@iq.uva.es.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bolado-Rodríguez
mesh_terms: D000096:Acetone / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D019149:Bioreactors; D000440:Butanols / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D003013:Clostridium / Q000378:metabolism*; D000431:Ethanol / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010126:Ozone / Q000737:chemistry*; D010843:Pichia / Q000378:metabolism*
chemical_list: D000440:Butanols; D000096:Acetone; D000431:Ethanol; D010126:Ozone
keywords: Butanol; Ethanol; Hydrolysates detoxification; Ozonolysis pretreatment; Sugarcane bagasse