title: Panacibacter ginsenosidivorans gen. nov., sp. nov., with ginsenoside converting activity isolated from soil of a ginseng field.
abstract: A ginsenoside-transforming bacterium, designated Gsoil 1550T, was isolated from soil of a ginseng field and subjected to a polyphasic taxonomic analysis. Colonies of strain Gsoil 1550T were yellow, of low convexity and with regular margin. Cells were long rods, 0.5-1.2 µm wide and 1.6-3 µm long. The isolate grew at 10-37 °C and at pH 5-9 on R2A agar medium; maximum growth occurred at 30 °C and pH 6-7. Phylogenetic study based on the 16S rRNA gene sequence positioned Gsoil 1550T in a distinct lineage in the family Chitinophagaceae, sharing 92.5-92.8 % sequence similarity with members of the closely related genera Terrimonas, Parafilimonas, Sediminibacterium and Parasegetibacter. Strain Gsoil 1550T contained menaquinone MK-7 as the predominant quinone, and iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 0 and iso-C15 : 1 G as major fatty acids. The DNA G+C content was 44.6 mol%. Strain Gsoil 1550T could be distinguished from other members of the family Chitinophagaceae by a number of chemotaxonomic and phenotypic characteristics. The major polar lipid of strain Gsoil 1550T was phosphatidylethanolamine. Based on this polyphasic taxonomic analysis, strain Gsoil 1150T represents a novel species within a new genus, for which the name Panacibacter ginsenosidivorans gen. nov., sp. nov. is proposed. The type strain of Panacibacter ginsenosidivorans is Gsoil 1550T (=KCTC 12658T=JCM 31452T).
authors.affiliation: Department of Biotechnology, Hankyoung National University, 327 Chungang-no, Anseong-si, Kyonggi-do 17579, Republic of Korea.|Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyoung National University, 327 Chungang-no, Anseong-si, Kyonggi-do 17579, Republic of Korea.
authors.forename: Muhammad Zubair
authors.initials: MZ
authors.lastname: Siddiqi
authors.affiliation: Chemical Research Department, Green Planet Co. Ltd, Okayama ken, tsushima nishizaka2 chome 5-41-203, Okayama, Japan.
authors.forename: Siddiqi
authors.initials: S
authors.lastname: Muhammad Shafi
authors.affiliation: Department of Biotechnology, Hankyoung National University, 327 Chungang-no, Anseong-si, Kyonggi-do 17579, Republic of Korea.|Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyoung National University, 327 Chungang-no, Anseong-si, Kyonggi-do 17579, Republic of Korea.
authors.forename: Kang Duk
authors.initials: KD
authors.lastname: Choi
authors.affiliation: Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyoung National University, 327 Chungang-no, Anseong-si, Kyonggi-do 17579, Republic of Korea.|Department of Biotechnology, Hankyoung National University, 327 Chungang-no, Anseong-si, Kyonggi-do 17579, Republic of Korea.
authors.forename: Wan-Taek
authors.initials: WT
authors.lastname: Im
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D036145:Ginsenosides / Q000378:metabolism*; D005894:Panax / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D036145:Ginsenosides; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7