title: Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions.
abstract: The cross-talk between dynamic microtubules and integrin-based adhesions to the extracellular matrix plays a crucial role in cell polarity and migration. Microtubules regulate the turnover of adhesion sites, and, in turn, focal adhesions promote the cortical microtubule capture and stabilization in their vicinity, but the underlying mechanism is unknown. Here, we show that cortical microtubule stabilization sites containing CLASPs, KIF21A, LL5β and liprins are recruited to focal adhesions by the adaptor protein KANK1, which directly interacts with the major adhesion component, talin. Structural studies showed that the conserved KN domain in KANK1 binds to the talin rod domain R7. Perturbation of this interaction, including a single point mutation in talin, which disrupts KANK1 binding but not the talin function in adhesion, abrogates the association of microtubule-stabilizing complexes with focal adhesions. We propose that the talin-KANK1 interaction links the two macromolecular assemblies that control cortical attachment of actin fibers and microtubules.
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Bouchet
authors.affiliation: School of Biosciences, University of Kent, Canterbury, United Kingdom.
authors.forename: Rosemarie E
authors.initials: RE
authors.lastname: Gough
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
authors.forename: York-Christoph
authors.initials: YC
authors.lastname: Ammon
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
authors.forename: Dieudonnée
authors.initials: D
authors.lastname: van de Willige
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Utrecht University, Utrecht, The Netherlands.|Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands.|Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.|The Netherlands Proteomics Centre, Utrecht University, Utrecht, The Netherlands.
authors.forename: Harm
authors.initials: H
authors.lastname: Post
authors.affiliation: Turku Centre for Biotechnology, University of Turku, Turku, Finland.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Jacquemet
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Utrecht University, Utrecht, The Netherlands.|Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands.|Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.|The Netherlands Proteomics Centre, Utrecht University, Utrecht, The Netherlands.
authors.forename: Af Maarten
authors.initials: AM
authors.lastname: Altelaar
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Utrecht University, Utrecht, The Netherlands.|Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands.|Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.|The Netherlands Proteomics Centre, Utrecht University, Utrecht, The Netherlands.
authors.forename: Albert Jr
authors.identifier: 0000-0002-2405-4404
authors.initials: AJ
authors.lastname: Heck
authors.affiliation: School of Biosciences, University of Kent, Canterbury, United Kingdom.
authors.forename: Benjamin T
authors.identifier: 0000-0002-3438-2807
authors.initials: BT
authors.lastname: Goult
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
authors.forename: Anna
authors.identifier: 0000-0002-9048-8614
authors.initials: A
authors.lastname: Akhmanova
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D022001:Focal Adhesions / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000378:metabolism*; D016608:Talin / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; C467739:KANK1 protein, human; D016608:Talin; D025521:Tumor Suppressor Proteins
keywords: biophysics; cell biology; focal adhesion; human; microtubule; structural biology; talin; KANK