title: Lipingzhangella halophila gen. nov., sp. nov., a new member of the family Nocardiopsaceae.
abstract: An alkaliphilic and halophilic actinomycete strain, designated EGI 80537T, was isolated from a saline-alkali soil sample of Xinjiang, north-west China and subjected to a taxonomic characterization using a polyphasic approach. Strain EGI 80537T formed reticulate long aerial hyphae. Whole-cell hydrolysates of the isolate contained meso-diaminopimelic acid as the cell-wall diamino acid and mannose as the diagnostic sugar. The major fatty acids identified were iso-C16 : 0, anteiso-C17 : 0 and 10-methyl-C18 : 0 (TBSA). The predominant menaquinones detected were MK-10(H8) and MK-10(H6). The G+C content of the genomic DNA of strain EGI 80537T was 67.6 mol%. Strain EGI 80537T showed the highest 16S rRNA gene sequence similarity to Allosalinactinospora lopnorensis CA15-2T (96.7 %). Phylogenetic analysis showed that strain EGI 80537T clustered with the members of the family Nocardiopsaceae. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, strain EGI 80537T represents a novel species of a new genus in the family Nocardiopsaceae, for which the name Lipingzhangella halophila gen. nov., sp. nov. is proposed. The type strain of the type species is EGI 80537T(=CGMCC 4.7224T= DSM 102030T).
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Zhang
authors.affiliation: New Drug Research & Development Center, North China Pharmaceutical Group Corporation, National Microbial Medicine Engineering & Research Center, Shijiazhuang 050015, PR China.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Lu
authors.affiliation: New Drug Research & Development Center, North China Pharmaceutical Group Corporation, National Microbial Medicine Engineering & Research Center, Shijiazhuang 050015, PR China.
authors.forename: Yan-Bo
authors.initials: YB
authors.lastname: Ding
authors.affiliation: China Tobacco Yunnan Industrial Co, Ltd., Kunming 650231, PR China.
authors.forename: Su-Juan
authors.initials: SJ
authors.lastname: Wang
authors.affiliation: China Tobacco Yunnan Industrial Co, Ltd., Kunming 650231, PR China.
authors.forename: Xing-Kui
authors.initials: XK
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.|College of Life Science, Liaoning Normal University, Dalian 116029, PR China.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.|Key Laboratory of Higher Quality and Efficient Cultivation and Security Control of Crops for Yunnan Province, Honghe University, Mengzi 661100, PR China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Guo
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Liu
authors.affiliation: China Tobacco Yunnan Industrial Co, Ltd., Kunming 650231, PR China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.|Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid