title: Circulating microRNAs and sudden cardiac arrest outcomes.
abstract: MicroRNAs (miRNAs) have regulatory functions in organs critical in resuscitation from sudden cardiac arrest due to ventricular fibrillation (VF-SCA); therefore, circulating miRNAs may be markers of VF-SCA outcome.
  
  
  
  We measured candidate miRNAs (N=45) in plasma using qRT-PCR among participants of a population-based VF-SCA study. Participants were randomly selected cases who died in the field (DF, n=15), died in hospital (DH, n=15), or survived to discharge (DC, n=15), and, age-, sex-, and race-matched controls (n=15). MiRNA levels were compared using ANOVA, t-tests, and fold-changes.
  
  
  
  Mean age of groups ranged from 66.9 to 69.7. Most participants were male (53-67%) and white (67%). Comparing cases to controls, plasma levels of 17 miRNAs expressed in heart, brain, liver, and other tissues (including miR-29c, -34a, -122, -145, -200a, -210, -499-5p, and -663b) were higher and three non-specific miRNAs lower (miR-221, -330-3p, and -9-5p). Among DH or DC compared with DF cases, levels of two miRNAs (liver-specific miR-122 and non-specific miR-205) were higher and two heart-specific miRNAs (miR-208b and -499-5p) lower. Among DC vs. DF cases, levels of three miRNAs (miR-122, and non-specific miR-200a and -205) were higher and four heart-specific miRNAs (miR-133a, -133b, -208b, and -499-5p) lower. Among DC vs. DH cases, levels of two non-specific miRNAs (miR-135a and -9-3p) were lower.
  
  
  
  Circulating miRNAs expressed in heart, brain, and other tissues differ between VF-SCA cases and controls and are related to resuscitation outcomes. Measurement of miRNAs may clarify mechanisms underlying resuscitation, improve prognostication, and guide development of therapies. Results require replication.
authors.affiliation: Cardiovascular Health Research Unit, Department of Medicine, University of Washington, Seattle, WA, USA; VA Puget Sound Health Care System, Seattle, WA, USA. Electronic address: lwander@u.washington.edu.
authors.forename: P L
authors.initials: PL
authors.lastname: Wander
authors.affiliation: Department of Epidemiology, University of Washington, Seattle, WA, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Enquobahrie
authors.affiliation: Department of Laboratory Medicine, University of Washington, Seattle, WA, USA.
authors.forename: C C
authors.initials: CC
authors.lastname: Pritchard
authors.affiliation: Department of Biostatistics, University of Washington, Seattle, WA, USA.
authors.forename: B
authors.initials: B
authors.lastname: McKnight
authors.affiliation: Department of Biostatistics, University of Washington, Seattle, WA, USA.
authors.forename: K
authors.initials: K
authors.lastname: Rice
authors.affiliation: Cardiovascular Health Research Unit, Department of Medicine, University of Washington, Seattle, WA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Christiansen
authors.affiliation: Cardiovascular Health Research Unit, Department of Medicine, University of Washington, Seattle, WA, USA.
authors.forename: R N
authors.initials: RN
authors.lastname: Lemaitre
authors.affiliation: Cardiovascular Health Research Unit, Department of Medicine, University of Washington, Seattle, WA, USA; Public Health-Seattle and King County, Emergency Medical Services Division, Seattle, WA, USA.
authors.forename: T
authors.initials: T
authors.lastname: Rea
authors.affiliation: New York Academy of Medicine, New York, NY, USA.
authors.forename: D
authors.initials: D
authors.lastname: Siscovick
authors.affiliation: Cardiovascular Health Research Unit, Department of Medicine, University of Washington, Seattle, WA, USA; Department of Epidemiology, University of Washington, Seattle, WA, USA; Division of Cardiology, Department of Medicine, University of Washington, Seattle, WA, USA.
authors.forename: N
authors.initials: N
authors.lastname: Sotoodehnia
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D016887:Cardiopulmonary Resuscitation / Q000401:mortality; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D058687:Out-of-Hospital Cardiac Arrest / Q000235:genetics* / Q000401:mortality; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: Cardiac arrest; Epidemiology; Epigenetics; Outcomes; microRNAs