title: Enhanced-Depth Imaging Optical Coherence Tomography of the Human Choroid In Vivo Compared With Histology After Enucleation.
abstract: This study compared in vivo enhanced-depth imaging optical coherence tomography (EDI-OCT) with ex vivo histology of the choroid in human eyes.
  
  
  
  Three eyes in three patients with advanced iris melanoma without posterior segment involvement underwent EDI-OCT less than 24 hours prior to enucleation and, in one eye, immediately after enucleation. Following fixation in 4% buffered formaldehyde and paraffin embedding, serial sections of the whole eye were cut horizontally, mounted, stained with hematoxylin-eosin and digitized. Alignment between histology and EDI-OCT was made on landmarks such as retinal vessel, the foveal depression, ciliary arteries, drusen, and nevi.
  
  
  
  Intra- and interindividual variations in relative choroidal thickness were comparable between the two modalities. After histologic fixation of the three melanoma eyes, the fullness of the choroidal vessels was reduced and subfoveal choroid thickness reduced to 56%, 45%, and 56%, respectively, of its in vivo thickness on EDI-OCT.
  
  
  
  There were no identifiable discrepancies in choroidal structural patterns between clinical EDI-OCT and histologic sections except that after enucleation and histologic fixation choroidal thickness was reduced to roughly half of its in vivo value, a phenomenon that may reflect the high content of blood vessels in the choroid.
authors.affiliation: Department of Ophthalmology, Rigshospitalet, Copenhagen, Denmark 2Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Xiao Qiang
authors.initials: XQ
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Rigshospitalet, Copenhagen, Denmark 2Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Steffen
authors.initials: S
authors.lastname: Heegaard
authors.affiliation: Department of Ophthalmology, Rigshospitalet, Copenhagen, Denmark 2Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Jens Folke
authors.initials: JF
authors.lastname: Kiilgaard
authors.affiliation: Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark 3Department of Ophthalmology, Zealand University Hospital, Roskilde, Denmark.
authors.forename: Inger Christine
authors.initials: IC
authors.lastname: Munch
authors.affiliation: Department of Ophthalmology, Rigshospitalet, Copenhagen, Denmark 2Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Michael
authors.initials: M
authors.lastname: Larsen
mesh_terms: D000368:Aged; D002829:Choroid / Q000473:pathology*; D015353:Eye Enucleation; D005260:Female; D005451:Fluorescein Angiography / Q000379:methods; D005500:Follow-Up Studies; D005654:Fundus Oculi; D006801:Humans; D007498:Iris / Q000473:pathology; D015811:Iris Neoplasms / Q000175:diagnosis* / Q000601:surgery; D008297:Male; D008545:Melanoma / Q000175:diagnosis* / Q000601:surgery; D008875:Middle Aged; D015203:Reproducibility of Results; D012171:Retinal Vessels / Q000473:pathology; D012189:Retrospective Studies; D041623:Tomography, Optical Coherence / Q000379:methods*