title: A microfluidic cell-trapping device for single-cell tracking of host-microbe interactions.
abstract: The impact of cellular individuality on host-microbe interactions is increasingly appreciated but studying the temporal dynamics of single-cell behavior in this context remains technically challenging. Here we present a microfluidic platform, InfectChip, to trap motile infected cells for high-resolution time-lapse microscopy. This approach allows the direct visualization of all stages of infection, from bacterial uptake to death of the bacterium or host cell, over extended periods of time. We demonstrate the utility of this approach by co-culturing an established host-cell model, Dictyostelium discoideum, with the extracellular pathogen Klebsiella pneumoniae or the intracellular pathogen Mycobacterium marinum. We show that the outcome of such infections is surprisingly heterogeneous, ranging from abortive infection to death of the bacterium or host cell. InfectChip thus provides a simple method to dissect the time-course of host-microbe interactions at the single-cell level, yielding new insights that could not be gleaned from conventional population-based measurements.
authors.affiliation: School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland. john.mckinney@epfl.ch.
authors.forename: Matthieu J
authors.initials: MJ
authors.lastname: Delincé
authors.affiliation: School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland. john.mckinney@epfl.ch.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Bureau
authors.affiliation: Department of Biochemistry, University of Geneva, Geneva, Switzerland. thierry.soldati@unige.ch.
authors.forename: Ana Teresa
authors.initials: AT
authors.lastname: López-Jiménez
authors.affiliation: Department for Cell Physiology and Metabolism, Centre Medical Universitaire, University of Geneva, Switzerland.
authors.forename: Pierre
authors.initials: P
authors.lastname: Cosson
authors.affiliation: Department of Biochemistry, University of Geneva, Geneva, Switzerland. thierry.soldati@unige.ch.
authors.forename: Thierry
authors.initials: T
authors.lastname: Soldati
authors.affiliation: School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland. john.mckinney@epfl.ch.
authors.forename: John D
authors.initials: JD
authors.lastname: McKinney
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000295:instrumentation*; D002478:Cells, Cultured; D018914:Cells, Immobilized; D018920:Coculture Techniques / Q000295:instrumentation*; D017076:Computer-Aided Design; D004023:Dictyostelium / Q000166:cytology / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D054884:Host-Pathogen Interactions; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D007711:Klebsiella pneumoniae / Q000166:cytology / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure; D056656:Lab-On-A-Chip Devices; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D019910:Mycobacterium marinum / Q000166:cytology / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure; D010586:Phagocytes / Q000166:cytology / Q000276:immunology / Q000382:microbiology / Q000469:parasitology; D010587:Phagocytosis; D059010:Single-Cell Analysis / Q000295:instrumentation*; D059008:Time-Lapse Imaging
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon