title: Effects of miR-26a on the expression of Beclin 1 in retinoblastoma cells.
abstract: The aim of this study was to evaluate the effects of miR-26a on Beclin 1 expression in retinoblastoma (RB) cell lines (Y79 and WERi-RB-1). RB cells were transfected with miR-26a mimic, antagomir-26a, or control mimic. The Beclin 1 mRNA and protein levels were detected by quantitative polymerase chain reaction and western blot, respectively. The activity of Beclin 1 3ꞌ-UTR reporter gene was detected with the luciferase assay. After transfection with miR-26a mimic, Beclin 1 mRNA and protein levels as well as the activity of the 3'-UTR reporter gene decreased. However, all were increased upon inhibition of miR-26a with antagomir-26a. Beclin 1 is the target of miR-26a in human RB cell lines Y79 and WERi-RB-1, and miR-26a inhibits the expression of Beclin 1 by reducing its mRNA and protein levels.
authors.affiliation: Department of Ophthalmology, West China Hospital of Sichuan University, Chengdu, Sichuan, China.|Department of Ophthalmology, AVIC 363 Hospital of Chengdu, Chengdu, Sichuan, China.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, West China Hospital of Sichuan University, Chengdu, Sichuan, China.
authors.forename: X M
authors.initials: XM
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, AVIC 363 Hospital of Chengdu, Chengdu, Sichuan, China.
authors.forename: D M
authors.initials: DM
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, AVIC 363 Hospital of Chengdu, Chengdu, Sichuan, China.
authors.forename: L
authors.initials: L
authors.lastname: Gan
authors.affiliation: Department of Ophthalmology, AVIC 363 Hospital of Chengdu, Chengdu, Sichuan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Qiao
mesh_terms: D020413:3' Untranslated Regions; D000071186:Beclin-1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019572:Retinal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012175:Retinoblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D000071186:Beclin-1; D035683:MicroRNAs; D012333:RNA, Messenger; C491997:BECN1 protein, human; C528163:MIRN26 microRNA, human