title: Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements.
abstract: Nondestructive prediction of ingredient contents of farm products is useful to ship and sell the products with guaranteed qualities. Here, near-infrared spectroscopy is used to predict nondestructively total sugar, total organic acid, and total anthocyanin content in each blueberry. The technique is expected to enable the selection of only delicious blueberries from all harvested ones. The near-infrared absorption spectra of blueberries are measured with the diffuse reflectance mode at the positions not on the calyx. The ingredient contents of a blueberry determined by high-performance liquid chromatography are used to construct models to predict the ingredient contents from observed spectra. Partial least squares regression is used for the construction of the models. It is necessary to properly select the pretreatments for the observed spectra and the wavelength regions of the spectra used for analyses. Validations are necessary for the constructed models to confirm that the ingredient contents are predicted with practical accuracies. Here we present a protocol to construct and validate the models for nondestructive prediction of ingredient contents in blueberries by near-infrared spectroscopy.
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology.
authors.forename: Wenming
authors.initials: W
authors.lastname: Bai
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology.
authors.forename: Norio
authors.initials: N
authors.lastname: Yoshimura
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology; masaot@cc.tuat.ac.jp.
authors.forename: Masao
authors.initials: M
authors.lastname: Takayanagi
authors.affiliation: Faculty of Agriculture, Tokyo University of Agriculture and Technology.
authors.forename: Jingai
authors.initials: J
authors.lastname: Che
authors.affiliation: Faculty of Agriculture, Tokyo University of Agriculture and Technology.
authors.forename: Naomi
authors.initials: N
authors.lastname: Horiuchi
authors.affiliation: Institute of Agriculture, Tokyo University of Agriculture and Technology.
authors.forename: Isao
authors.initials: I
authors.lastname: Ogiwara
mesh_terms: D029798:Blueberry Plants; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D016018:Least-Squares Analysis; D008962:Models, Theoretical; D019265:Spectroscopy, Near-Infrared