title: Comparison of arsenate reduction and release by three As(V)-reducing bacteria isolated from arsenic-contaminated soil of Inner Mongolia, China.
abstract: Arsenic (As) contamination has become a worldwide environmental problem: arsenite (As(Ⅲ)) especially has posed a major threat to human health. Here, we report the first three isolates of anaerobic As(Ⅴ)-reducing bacterial strains (strains JQ, DJ-3 and DJ-4) from a soil sample containing 48.7% of total As in the form of As(III) collected in Chifeng, Inner Mongolia, China. Strains JQ, DJ-3 and DJ-4 were phylogenetically closely related to Bacillus, Desulfitobacterium and Exiguobacterium, respectively. Among these strains, JQ and DJ-3 have the arsC gene, DJ-4 possesses the arrA gene. The three strains could all resist and reduce high concentrations of As(Ⅴ) under anoxic conditions. The order of resistance to As(Ⅴ) was DJ-3 > JQ > DJ-4. Strain DJ-3 not only possesses the strongest resistance to As(Ⅴ) but could also reduce 53% of the As(Ⅴ) to As(III) in the treatment of 60 mM As(Ⅴ) in 5 d. All three strains could release As from goethite; strain DJ-4 has the highest ability to promote the release of As (90.5%) from goethite. These results suggested that strains JQ, DJ-3 and DJ-4 may play an important role in the mobilization and transformation of As in soil.
authors.affiliation: College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, People's Republic of China; Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, People's Republic of China. Electronic address: caixiaolin13@mails.ucas.ac.cn.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Cai
authors.affiliation: College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, People's Republic of China; Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, People's Republic of China. Electronic address: zhangzhennan10@mails.ucas.ac.cn.
authors.forename: Zhennan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, People's Republic of China; Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, People's Republic of China. Electronic address: yinnaiyi12@mails.ucas.ac.cn.
authors.forename: Naiyi
authors.initials: N
authors.lastname: Yin
authors.affiliation: College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, People's Republic of China; Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, People's Republic of China. Electronic address: duhuili14@mails.ucas.ac.cn.
authors.forename: Huili
authors.initials: H
authors.lastname: Du
authors.affiliation: College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, People's Republic of China; Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, People's Republic of China. Electronic address: lizejiao15@mails.ucas.ac.cn.
authors.forename: Zejiao
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, People's Republic of China; Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, People's Republic of China. Electronic address: cuiyanshan@ucas.ac.cn.
authors.forename: Yanshan
authors.initials: Y
authors.lastname: Cui
mesh_terms: D001149:Arsenates / Q000032:analysis / Q000378:metabolism*; D001151:Arsenic / Q000032:analysis / Q000378:metabolism; D018053:Arsenites / Q000032:analysis / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002681:China; D005798:Genes, Bacterial; D058085:Iron Compounds / Q000737:chemistry; D008903:Minerals / Q000737:chemistry; D010084:Oxidation-Reduction; D010802:Phylogeny; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
chemical_list: D001149:Arsenates; D018053:Arsenites; D058085:Iron Compounds; D008903:Minerals; D012989:Soil Pollutants; C094886:goethite; C015001:arsenite; D001151:Arsenic; C025657:arsenic acid
keywords: Arsenate reduction; As release; As(Ⅴ)-reducing bacteria; Goethite