title: 2-Hydrazinoquinoline: a reactive matrix for matrix-assisted laser desorption/ionization mass spectrometry to detect gaseous carbonyl compounds.
abstract: The sensitivity, range of applications, and reaction mechanism of 2-hydrazinoquinoline as a reactive matrix for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) were examined. Using a reaction chamber (125L) equipped with a stirring fan and a window for moving the MALDI-MS plate and volatile samples in and out, the sensitivities of 2-hydrazinoquinoline to gaseous aldehydes (formaldehyde, acetaldehyde, propionaldehyde, and n-butyraldehyde) and ketones (acetone, methyl ethyl ketone, and methyl isobutyl ketone) were determined to be at least parts per million (ppm) levels. On the other hand, carboxylic acids (formic acid, acetic acid, propionic acid, and butyric acid) and esters (ethyl acetate, pentyl acetate, isoamyl acetate, and methyl salicylate) could not be detected by 2-hydrazinoquinoline in MALDI-MS. In addition to 2,4-dinitrophenylhydrazine, a common derivatization reagent for analyzing carbonyl compounds quantitatively in gas chromatography and liquid chromatography, the dissolution of 2-hydrazinoquinoline in an acidic solution, such as trifluoroacetic acid, was essential for its function as a reactive matrix for MALDI- MS.
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan. yasushi.shigeri@aist.go.jp.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shigeri
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Kamimura
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan. m-ando@aist.go.jp.
authors.forename: Masanori
authors.initials: M
authors.lastname: Ando
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan. k-uegaki@aist.go.jp.
authors.forename: Koichi
authors.initials: K
authors.lastname: Uegaki
authors.affiliation: Department of Chemistry and Materials Engineering, Kansai University, Yamate-cho, Suita, Osaka, 564-8680, Japan. sato- hiroaki@aist.go.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sato
authors.affiliation: Institute for Materials Chemistry and Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, Fukuoka, 819-0395, Japan. tanif@ms.ifoc.kyushu-u.ac.jp.
authors.forename: Fumito
authors.initials: F
authors.lastname: Tani
authors.affiliation: Department of Chemistry and Materials Engineering, Kansai University, Yamate-cho, Suita, Osaka, 564-8680, Japan. arak@kansai- u.ac.jp.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Arakawa
authors.affiliation: Research Institute for Material and Chemical Measurement, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 3, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8563, Japan. t.kinumi@aist.go.jp.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Kinumi