title: Spotlight on the three main hepatic fibrogenic cells in HCV-infected patients: Multiple immunofluorescence and ultrastructure study.
abstract: The present work deals with the simultaneous ultrastructure and triple immunofluorescence study of the three main hepatic fibrogenic cells, hepatic stellate cell, myofibroblast (MF), and fibroblast, in a group of hepatitis C virus (HCV) RNA positive patients, as their exact interrelation behavior in vivo with the progress of hepatic fibrosis is still inadequate. In this study, for the first time, cells having the morphological characteristic of MF and not bone marrow fibrocytes were revealed in liver portal vessels. This necessitates the reevaluation of the available knowledge concerning bone marrow fibrocyte. Also, the distribution, cellular interrelations, and the fate of MF were highlighted.
authors.affiliation: a Electron Microscopy Research Department (Pathology) , Theodor Bilharz Research Institute , Giza , Egypt.
authors.forename: Soheir S
authors.initials: SS
authors.lastname: Mansy
authors.affiliation: b Pathology Department , Theodor Bilharz Research Institute , Giza , Egypt.
authors.forename: Mona M
authors.initials: MM
authors.lastname: Nosseir
authors.affiliation: a Electron Microscopy Research Department (Pathology) , Theodor Bilharz Research Institute , Giza , Egypt.
authors.forename: May M
authors.initials: MM
authors.lastname: Othman
authors.affiliation: c Immunology Department , Theodor Bilharz Research Institute , Giza , Egypt.
authors.forename: Mona A
authors.initials: MA
authors.lastname: Zoheiry
authors.affiliation: d Hepatogastroenterology Department , Theodor Bilharz Research Institute , Giza , Egypt.
authors.forename: Mohammed F
authors.initials: MF
authors.lastname: Guda
authors.affiliation: a Electron Microscopy Research Department (Pathology) , Theodor Bilharz Research Institute , Giza , Egypt.
authors.forename: Hoda A
authors.initials: HA
authors.lastname: Yehia
authors.affiliation: d Hepatogastroenterology Department , Theodor Bilharz Research Institute , Giza , Egypt.
authors.forename: Moataz H
authors.initials: MH
authors.lastname: Hassanein
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D055166:Hepatic Stellate Cells / Q000648:ultrastructure*; D006526:Hepatitis C / Q000150:complications / Q000473:pathology*; D006801:Humans; D006820:Hyaluronic Acid; D008103:Liver Cirrhosis / Q000473:pathology* / Q000821:virology; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D058628:Myofibroblasts / Q000648:ultrastructure*
chemical_list: D006820:Hyaluronic Acid
keywords: Bone marrow fibrocyte; fibroblast; hepatic stellate cell; liver fibrosis; myofibroblast