title: Intratracheal therapy with autologous bone marrow-derived mononuclear cells reduces airway inflammation in horses with recurrent airway obstruction.
abstract: This research evaluated the effects of bone marrow-derived mononuclear cells (BMMCs) on the inflammatory process in the equine recurrent airway obstruction (RAO). Eight horses in RAO clinical score were divided into cell therapy group (Gcel) treated with a single intratracheal dose of BMMCs, and dexamethasone group (Gdex) treated with 21days of oral dexamethasone. The horses were clinically revaluated on days 7 and 21, together with cytological evaluation of the BALF, and detection of inflammatory markers (interleukins [IL]-10, -4, and -17, and interferon γ and α). There were decreases in respiratory effort and clinical score on days 7 and 21(p<0.05) for both groups. The percentage of neutrophils decreased and macrophages increased on days 7 and 21 (p<0.005) in both groups. IL-10 levels increased in the Gcel group on day 21 compared to days 0 and 7 (p<0.05), but this was not observed in the Gdex group. The quantification of IL-4, IL-17, IFN-γ, and IFN-α did not change between evaluations in both groups. These preliminary results suggest that BMMCs may ameliorate the inflammatory response of RAO.
authors.affiliation: Department of Animal Science, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Prado Velho, Curitiba, Paraná 80215-901, Brazil.
authors.forename: Fernanda C M
authors.initials: FC
authors.lastname: Barussi
authors.affiliation: Department of Animal Science, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Prado Velho, Curitiba, Paraná 80215-901, Brazil.
authors.forename: Fernanda Z
authors.initials: FZ
authors.lastname: Bastos
authors.affiliation: School of Medicine, Experimental Laboratory for Cell Culture, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Prado Velho, Curitiba, Paraná 80215-901, Brazil.
authors.forename: Lidiane M B
authors.initials: LM
authors.lastname: Leite
authors.affiliation: School of Medicine, Experimental Laboratory for Cell Culture, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Prado Velho, Curitiba, Paraná 80215-901, Brazil.
authors.forename: Felipe Y I
authors.initials: FY
authors.lastname: Fragoso
authors.affiliation: School of Medicine, Experimental Laboratory for Cell Culture, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Prado Velho, Curitiba, Paraná 80215-901, Brazil.
authors.forename: Alexandra C
authors.initials: AC
authors.lastname: Senegaglia
authors.affiliation: School of Medicine, Experimental Laboratory for Cell Culture, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Prado Velho, Curitiba, Paraná 80215-901, Brazil.
authors.forename: Paulo R S
authors.initials: PR
authors.lastname: Brofman
authors.affiliation: Department of Physiology, Universidade Federal do Paraná, Av. Coronel Francisco Heráclito dos Santos, 210, Jardim das Americas, Curitiba, Paraná 81531-970, Brazil.
authors.forename: Anita
authors.initials: A
authors.lastname: Nishiyama
authors.affiliation: Department of Animal Science, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Prado Velho, Curitiba, Paraná 80215-901, Brazil.
authors.forename: Cláudia T
authors.initials: CT
authors.lastname: Pimpão
authors.affiliation: Department of Animal Science, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Prado Velho, Curitiba, Paraná 80215-901, Brazil. Electronic address: p.michelotto@pucpr.br.
authors.forename: Pedro V
authors.initials: PV
authors.lastname: Michelotto
mesh_terms: D000402:Airway Obstruction / Q000150:complications / Q000601:surgery / Q000662:veterinary; D000704:Analysis of Variance; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D003907:Dexamethasone / Q000627:therapeutic use; D005260:Female; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006736:Horses; D007249:Inflammation / Q000150:complications / Q000601:surgery / Q000662:veterinary; D000069555:Injection, Intratympanic / Q000379:methods; D016753:Interleukin-10 / Q000378:metabolism; D008264:Macrophages / Q000502:physiology; D008297:Male; D009504:Neutrophils / Q000502:physiology; D014182:Transplantation, Autologous
chemical_list: D016753:Interleukin-10; D003907:Dexamethasone
keywords: Bronchoalveolar lavage; Heaves; Horses; Interleukins; Neutrophils; Pulmonary inflammation