title: Assessment of Apoptosis Regulating Factors BCL-2 and Fas Antigens on Malignant and Normal Plasma Cells.
abstract: Multiple myeloma (MM) is characterised by slow proliferation of malignant plasma cells and their accumulation within the bone marrow. The dysregulation of programmed cell death (apoptosis) is a very important mechanism in the pathogenesis of this tumour. It prompted us to investigate the apoptosis regulating factors such as the pro-apoptotic Fas antigen and the anti-apoptotic protein BCL-2 on bone marrow malignant plasma cells in untreated patients with newly diagnosed MM and to compare them with their normal counterparts-plasma cells isolated from bone marrow of healthy individuals. Twenty-nine MM patients and 16 healthy persons were studied. Bone marrow mononuclear cells were isolated, indicated by monoclonal antibodies and analysed using the flow cytometry method. There was no statistically significant difference in BCL-2 expression in plasma cells between patients and control groups. However the percentage of BCL-2 positive cells was significantly related to the clinical stage of the disease. We detected statistically significant lower percentage of Fas positive cells in the patient group than in control. We concluded that in MM at diagnosis the expression of BCL-2 in bone marrow malignant plasma cells was comparable to normal plasma cells but expression of Fas antigen on these cells was lower. It suggests that down regulation of Fas and normal regulation of BCL-2 may be implicated for myeloma cell survival and their escape from apoptosis in vivo.
authors.affiliation: a Department of Haematology , University School of Medicine , Jaczewskiego 8, 20-950 , Lublin , Poland.
authors.forename: A
authors.initials: A
authors.lastname: Dmoszynska
authors.affiliation: b Department of Clinical Immunology , University School of Medicine , Lublin , Poland.
authors.forename: M
authors.initials: M
authors.lastname: Podhorecka
authors.affiliation: a Department of Haematology , University School of Medicine , Jaczewskiego 8, 20-950 , Lublin , Poland.
authors.forename: A
authors.initials: A
authors.lastname: Khmaj
authors.affiliation: a Department of Haematology , University School of Medicine , Jaczewskiego 8, 20-950 , Lublin , Poland.
authors.forename: A
authors.initials: A
authors.lastname: Surdacka
authors.affiliation: b Department of Clinical Immunology , University School of Medicine , Lublin , Poland.
authors.forename: A
authors.initials: A
authors.lastname: Walter-Croneck
authors.affiliation: a Department of Haematology , University School of Medicine , Jaczewskiego 8, 20-950 , Lublin , Poland.
authors.forename: J
authors.initials: J
authors.lastname: Rolinski
keywords: Apoptosis; BCL-2; Fas; Multiple myeloma