title: Secretome from resident cardiac stromal cells stimulates proliferation, cardiomyogenesis and angiogenesis of progenitor cells.
abstract: In the heart, tissue-derived signals play a central role on recruiting/activating stem cell sources to induce cardiac lineage specification for maintenance of tissue homeostasis and repair. Cardiac resident stromal cells (CRSCs) may play a pivotal role in cardiac repair throughout their secretome. Here, we performed the characterization of CRSCs and their secretome by analyzing the composition of their culture-derived extracellular matrix (ECM) and conditioned medium (CM) and by investigating their potential effect on adipose-derived stem cell (ADSC) and progenitor cell behavior. We confirmed that CRSCs are a heterogeneous cell population whose secretome is composed by proteins related to cellular growth, immune response and cardiovascular development and function. We also observed that CRSC secretome was unable to change the behavior of ADSCs, except for proliferation. Additionally, CM from CRSCs demonstrated the potential to drive proliferation and cardiac differentiation of H9c2 cells and also the ability to induce angiogenesis in vitro. Our data suggest that the CRSCs can be a source of important modulating signals for cardiac progenitor cell recruitment/activation.
authors.affiliation: Laboratory of Basic Biology of Stem Cells, Carlos Chagas Institute, Fiocruz-Parana, Curitiba, 81350-010, Brazil. Electronic address: thamilelreus@gmail.com.
authors.forename: Thamile Luciane
authors.initials: TL
authors.lastname: Reus
authors.affiliation: Laboratory of Basic Biology of Stem Cells, Carlos Chagas Institute, Fiocruz-Parana, Curitiba, 81350-010, Brazil. Electronic address: nany.robert@gmail.com.
authors.forename: Anny Waloski
authors.initials: AW
authors.lastname: Robert
authors.affiliation: Bank of Valve Homografts, Santa Casa Hospital, Curitiba, 80215-901, Brazil. Electronic address: mcosta13@icloud.com.
authors.forename: Marise Brenner Affonso
authors.initials: MB
authors.lastname: Da Costa
authors.affiliation: Laboratory of Basic Biology of Stem Cells, Carlos Chagas Institute, Fiocruz-Parana, Curitiba, 81350-010, Brazil. Electronic address: alessandra_aguiar@fiocruz.br.
authors.forename: Alessandra Melo
authors.initials: AM
authors.lastname: de Aguiar
authors.affiliation: Laboratory of Basic Biology of Stem Cells, Carlos Chagas Institute, Fiocruz-Parana, Curitiba, 81350-010, Brazil. Electronic address: mstimamiglio@fiocruz.br.
authors.forename: Marco Augusto
authors.initials: MA
authors.lastname: Stimamiglio
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D005109:Extracellular Matrix; D006801:Humans; D032386:Myoblasts, Cardiac / Q000502:physiology*; D009206:Myocardium / Q000166:cytology*; D055571:Secretory Pathway / Q000502:physiology*; D017154:Stromal Cells / Q000502:physiology
chemical_list: D017077:Culture Media, Conditioned
keywords: Angiogenesis; Cardiac resident stromal cells; Conditioned medium; Extracellular matrix; Secretome; Stem cells