title: Quercetin 3,7-O-dimethyl ether from Siegesbeckia pubescens suppress the production of inflammatory mediators in lipopolysaccharide-induced macrophages and colon epithelial cells.
abstract: Siegesbeckia pubescens (Compositae) is an annual herb indigenous to Korean mountainous regions. Recent reports have been issued on some compounds derived from S. pubescens for its anti-inflammatory activity or mode of action. The quercetin 3,7-O-dimethyl ether (QDE) isolated from the herbs of S. pubescens suppressed the lipopolysaccharide (LPS)-induced nitric oxide and inducible nitric oxide synthase (iNOS) protein production in mouse macrophages. QDE downregulated pro-inflammatory cytokines such as interleukin (IL)-6, IL-1β, tumor necrosis factor -α levels in LPS-stimulated macrophages. Also, QDE decreased the expression of LPS-induced iNOS and cyclooxygenase-2 (COX-2) protein and the production of IL-8 in LPS-induced HT-29 cells. Macrophages and colon epithelial cells are important for regulating the colon immune systems, thus QDE may regulate inflammatory colon disease via LPS-induced inflammation in macrophages and colon epithelial cells. QDE, anti-inflammatory constituent of S. pubescens herbs, can be expected to be a potential candidate for therapeutics against inflammatory bowel disease.
authors.affiliation: a College of Pharmacy , Sunchon National University , Suncheon-si , Republic of Korea.
authors.forename: Seul-Gi
authors.initials: SG
authors.lastname: Lee
authors.affiliation: a College of Pharmacy , Sunchon National University , Suncheon-si , Republic of Korea.
authors.forename: Mihye
authors.initials: M
authors.lastname: Kim
authors.affiliation: a College of Pharmacy , Sunchon National University , Suncheon-si , Republic of Korea.
authors.forename: Cho Een
authors.initials: CE
authors.lastname: Kim
authors.affiliation: a College of Pharmacy , Sunchon National University , Suncheon-si , Republic of Korea.
authors.forename: Jisu
authors.initials: J
authors.lastname: Kang
authors.affiliation: a College of Pharmacy , Sunchon National University , Suncheon-si , Republic of Korea.
authors.forename: Hyunji
authors.initials: H
authors.lastname: Yoo
authors.affiliation: b College of Pharmacy and Research Institute of Pharmaceutical Sciences , Seoul National University , Seoul , Republic of Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
authors.affiliation: a College of Pharmacy , Sunchon National University , Suncheon-si , Republic of Korea.
authors.forename: Mina
authors.initials: M
authors.lastname: Lee
keywords: IBD; colon epithelial cells; inflammation; macrophages