title: Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications.
abstract: Textile-based technologies are powerful routes for the production of three-dimensional porous architectures for tissue engineering applications because of their feasibility and possibility for scaling-up. Herein, the use of knitting technology to produce polybutylene succinate fibre-based porous architectures is described. Furthermore, different treatments have been applied to functionalize the surface of the scaffolds developed: sodium hydroxide etching, ultraviolet radiation exposure in an ozone atmosphere and grafting (acrylic acid, vinyl phosphonic acid and vinyl sulphonic acid) after oxygen plasma activation as a way to tailor cell adhesion. A possible effect of the applied treatments on the bulk properties of the textile scaffolds has been considered and thus tensile tests in dry and hydrated states were also carried out. The microscopy results indicated that the surface morphology and roughness were affected by the applied treatments. The X-ray photoelectron spectroscopy and contact angle measurements showed the incorporation of oxygen-containing groups and higher surface free energy as result of the surface treatments applied. The DNA quantification and scanning electron microscopy analysis revealed that these modifications enhanced cell adhesion and altered cell morphology. Generally, sodium hydroxide treatment altered most significantly the surface properties, which in turn resulted in a high number of cells adherent to these surfaces. Based on the results obtained, the proposed surface treatments are appropriate to modify polybutylene succinate knitting scaffolds, influencing cell adhesion and its potential for use in tissue engineering applications. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.|ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
authors.forename: Viviana P
authors.identifier: 0000-0002-3679-0759
authors.initials: VP
authors.lastname: Ribeiro
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.|ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
authors.forename: Lília R
authors.initials: LR
authors.lastname: Almeida
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.|ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
authors.forename: Ana R
authors.initials: AR
authors.lastname: Martins
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.|ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
authors.forename: Iva
authors.initials: I
authors.lastname: Pashkuleva
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.|ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
authors.forename: Alexandra P
authors.initials: AP
authors.lastname: Marques
authors.affiliation: CeNTI, Centre for Nanotechnology and Smart Materials, V. N. Famalicão, Portugal.
authors.forename: Ana S
authors.initials: AS
authors.lastname: Ribeiro
authors.affiliation: CeNTI, Centre for Nanotechnology and Smart Materials, V. N. Famalicão, Portugal.
authors.forename: Carla J
authors.initials: CJ
authors.lastname: Silva
authors.affiliation: CITEVE, Technological Centre for Textile and Clothing Industry, V. N. Famalicão, Portugal.
authors.forename: Graça
authors.initials: G
authors.lastname: Bonifácio
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.|ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
authors.forename: Rui A
authors.initials: RA
authors.lastname: Sousa
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.|ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.|CBQF-Centre for Biotechnology and Fine Chemistry, Portuguese Catholic University, Porto, Portugal.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Oliveira
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.|ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002072:Butylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D013718:Tensile Strength; D013784:Textiles; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D002072:Butylene Glycols; D011108:Polymers; C089797:bionole; D014867:Water; D004247:DNA
keywords: biomedical; biotextile; knitted structure; polybutylene succinate; scaffold; surface modification; tissue engineering