title: CTCF and Sgfl1 proteins form alternative complexes with ENY2 proteins.
abstract: ENY2 is a multifunctional protein, a component of the SAGA complex (protein complex involved in the regulation of transcription, possessing histone-acetyltransferase and H2B-histone-deubiquitinylating activities [1]) and TREX-2 (complex involved in the mRNA nuclear export [2]). Besides this, the interactions of ENY2 with DNA-binding insulator proteins Su(Hw) and dCTCF have been described as essential for the barrier activity of corresponding insulators [3, 4]. In this study, we described the formation of mutually exclusive complexes of ENY2 with insulator proteins and Sgfl1-a component of the SAGA complex, direct binding partner for ENY2.
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, ul. Vavilova 34/5, Moscow, 119334, Russia. errinaceus@rambler.ru.
authors.forename: A N
authors.initials: AN
authors.lastname: Bonchuk
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, ul. Vavilova 34/5, Moscow, 119334, Russia.
authors.forename: P G
authors.initials: PG
authors.lastname: Georgiev
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, ul. Vavilova 34/5, Moscow, 119334, Russia.
authors.forename: O G
authors.initials: OG
authors.lastname: Maksimenko
mesh_terms: D000818:Animals; D000076246:CCCTC-Binding Factor; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000378:metabolism; D006801:Humans; D062105:Molecular Docking Simulation; D019906:Nuclear Magnetic Resonance, Biomolecular; D012097:Repressor Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D029721:Drosophila Proteins; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; C489245:SGF11 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C527172:Sgf11 protein, Drosophila; D014157:Transcription Factors