title: TRAIP is involved in chromosome alignment and SAC regulation in mouse oocyte meiosis.
abstract: Recent whole-exome sequencing (WES) studies demonstrated that TRAIP is associated with primordial dwarfism. Although TRAIP was partially studied in mitosis, its function in oocyte meiosis remained unknown. In this study, we investigated the roles of TRAIP during mouse oocyte meiosis. TRAIP was stably expressed during oocytes meiosis and co-localized with CREST at the centromere region. Knockdown of TRAIP led to DNA damage, as revealed by the appearance of γH2AX. Although oocytes meiotic maturation was not affected, the proportions of misaligned chromosomes and aneuploidy were elevated after TRAIP knockdown, suggesting TRAIP is required for stable kinetochore-microtubule (K-MT) attachment. TRAIP knockdown decreased the accumulation of Mad2 on centromeres, potentially explaining why oocyte maturation was not affected following formation of DNA lesions. Securin, a protein which was prevent from precocious degradation by Mad2, was down-regulated after TRAIP knockdown. Inhibition of TRAIP by microinjection of antibody into pro-metaphase I (pro-MI) stage oocytes resulted in precocious first polar body (PB1) extrusion, and live-cell imaging clearly revealed misaligned chromosomes after TRAIP knockdown. Taken together, these data indicate that TRAIP plays important roles in oocyte meiosis regulation.
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, No. 49 North HuaYuan Road, HaiDian District, Beijing 100191, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China.
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China.
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Ren
authors.affiliation: Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yuan
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, No. 49 North HuaYuan Road, HaiDian District, Beijing 100191, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Yan
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, No. 49 North HuaYuan Road, HaiDian District, Beijing 100191, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
mesh_terms: D000818:Animals; D002503:Centromere / Q000235:genetics / Q000378:metabolism*; D033481:Chromosomes, Mammalian / Q000235:genetics / Q000378:metabolism*; D005260:Female; D059566:M Phase Cell Cycle Checkpoints / Q000502:physiology*; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000166:cytology / Q000201:enzymology*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D044767:Ubiquitin-Protein Ligases