title: Association between STR -794 CATT5-8 and SNP -173 G/C polymorphisms in the MIF gene and Lepromatous Leprosy in Mestizo patients of western Mexico.
abstract: Lepromatous Leprosy (LL) is the most common presentation of leprosy in Mexico. LL patients are unable to activate an effective inflammatory response against Mycobacterium leprae probably due to the genetics of the host. Macrophage Migration Inhibitory Factor (MIF) is important to trigger inflammation processes. Two polymorphisms have been reported for human MIF: STR -794 CATT5-8 and SNP -173 G/C. 7-8 CATT repeats at -794 and the C allele at -173 increase the expression of MIF. We aim to determine the association between the polymorphisms in MIF gene and LL. We carried a case and controls study with 100 Mexican LL patients and 100 healthy subjects (HS). PCR was used for genotyping of STR -794 CATT5-8 polymorphism and PCR-RFLP for -173 G/C. We found that LL patients possess high -794 CATT repeats (47.1%) more often than HS (32.7%). In conclusion, a MIF polymorphism is associated with susceptibility to LL in Western Mexican population.
authors.affiliation: Doctorado en Ciencias Biomédicas con Orientación en Inmunología, Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, México, Sierra Mojada 950, Col. Independencia, 44340 Guadalajara, Jalisco, Mexico; Centro de Investigación en Inmunología y Dermatología/Universidad de Guadalajara, México, Av. Federalismo Norte 3102, Col. Atemajac del Valle, 45190 Zapopan, Jalisco, Mexico. Electronic address: mamartinezg90@gmail.com.
authors.forename: M A
authors.initials: MA
authors.lastname: Martinez-Guzman
authors.affiliation: Centro de Investigación en Inmunología y Dermatología/Universidad de Guadalajara, México, Av. Federalismo Norte 3102, Col. Atemajac del Valle, 45190 Zapopan, Jalisco, Mexico. Electronic address: bell2000_mx@yahoo.com.
authors.forename: A
authors.initials: A
authors.lastname: Alvarado-Navarro
authors.affiliation: Centro de Investigación en Inmunología y Dermatología/Universidad de Guadalajara, México, Av. Federalismo Norte 3102, Col. Atemajac del Valle, 45190 Zapopan, Jalisco, Mexico. Electronic address: analauraps@hotmail.com.
authors.forename: A L
authors.initials: AL
authors.lastname: Pereira-Suarez
authors.affiliation: Doctorado en Ciencias Biomédicas con Orientación en Inmunología, Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, México, Sierra Mojada 950, Col. Independencia, 44340 Guadalajara, Jalisco, Mexico. Electronic address: biologiamolecular@hotmail.com.
authors.forename: J F
authors.initials: JF
authors.lastname: Muñoz-Valle
authors.affiliation: Centro de Investigación en Inmunología y Dermatología/Universidad de Guadalajara, México, Av. Federalismo Norte 3102, Col. Atemajac del Valle, 45190 Zapopan, Jalisco, Mexico. Electronic address: mfafutis@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Fafutis-Morris
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D015440:Leprosy, Lepromatous / Q000235:genetics*; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D008297:Male; D008800:Mexico; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D008263:Macrophage Migration-Inhibitory Factors; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human
keywords: Leprosy; Macrophage Migration Inhibitory Factor; Polymorphisms