title: Mitochondrial adaptations to high-volume exercise training are rapidly reversed after a reduction in training volume in human skeletal muscle.
abstract: Increased mitochondrial content and respiration have both been reported after exercise training. However, no study has directly compared how different training volumes influence mitochondrial respiration and markers of mitochondrial biogenesis. Ten healthy men performed high-intensity interval cycling during 3 consecutive training phases; 4 wk of normal-volume training (NVT; 3/wk), followed by 20 d of high-volume training (HVT; 2/d) and 2 wk of reduced-volume training (RVT; 5 sessions). Resting biopsy samples (vastus lateralis) were obtained at baseline and after each phase. No mitochondrial parameter changed after NVT. After HVT, mitochondrial respiration and citrate synthase activity (∼40-50%), as well as the protein content of electron transport system (ETS) subunits (∼10-40%), and that of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), NRF1, mitochondrial transcription factor A (TFAM), PHF20, and p53 (∼65-170%) all increased compared to baseline; mitochondrial specific respiration remained unchanged. After RVT, all the mitochondrial parameters measured except citrate synthase activity (∼36% above initial) were not significantly different compared to baseline (all P > 0.05). Our findings demonstrate that training volume is an important determinant of training-induced mitochondrial adaptations and highlight the rapid reversibility of human skeletal muscle to a reduction in training volume.-Granata, C., Oliveira, R. S. F., Little, J. P., Renner, K., Bishop, D. J. Mitochondrial adaptations to high-volume exercise training are rapidly reversed after a reduction in training volume in human skeletal muscle.
authors.affiliation: Institute of Sport, Exercise, and Active Living, College of Sport and Exercise Science, Victoria University, Melbourne, Victoria, Australia.
authors.forename: Cesare
authors.initials: C
authors.lastname: Granata
authors.affiliation: Institute of Sport, Exercise, and Active Living, College of Sport and Exercise Science, Victoria University, Melbourne, Victoria, Australia.
authors.forename: Rodrigo S F
authors.initials: RS
authors.lastname: Oliveira
authors.affiliation: School of Health and Exercise Sciences, University of British Columbia Okanagan, Kelowna, British Columbia, Canada; and.
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Little
authors.affiliation: Department of Internal Medicine III, University Hospital of Regensburg, Regensburg, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Renner
authors.affiliation: Institute of Sport, Exercise, and Active Living, College of Sport and Exercise Science, Victoria University, Melbourne, Victoria, Australia; david.bishop@vu.edu.au.
authors.forename: David J
authors.initials: DJ
authors.lastname: Bishop
mesh_terms: D000064:Acclimatization; D000222:Adaptation, Physiological / Q000502:physiology*; D000293:Adolescent; D000328:Adult; D015444:Exercise / Q000502:physiology* / Q000523:psychology; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism; D055815:Young Adult
chemical_list: D006360:Heat-Shock Proteins; D047495:PPAR gamma
keywords: PGC-1α; PHF20; mitochondrial biogenesis; mitochondrial respiration; p53