title: 100-fold but not 50-fold dystrophin overexpression aggravates electrocardiographic defects in the mdx model of Duchenne muscular dystrophy.
abstract: Dystrophin gene replacement holds the promise of treating Duchenne muscular dystrophy. Supraphysiological expression is a concern for all gene therapy studies. In the case of Duchenne muscular dystrophy, Chamberlain and colleagues found that 50-fold overexpression did not cause deleterious side effect in skeletal muscle. To determine whether excessive dystrophin expression in the heart is safe, we studied two lines of transgenic mdx mice that selectively expressed a therapeutic minidystrophin gene in the heart at 50-fold and 100-fold of the normal levels. In the line with 50-fold overexpression, minidystrophin showed sarcolemmal localization and electrocardiogram abnormalities were corrected. However, in the line with 100-fold overexpression, we not only detected sarcolemmal minidystrophin expression but also observed accumulation of minidystrophin vesicles in the sarcoplasm. Excessive minidystrophin expression did not correct tachycardia, a characteristic feature of Duchenne muscular dystrophy. Importantly, several electrocardiogram parameters (QT interval, QRS duration and the cardiomyopathy index) became worse than that of mdx mice. Our data suggests that the mouse heart can tolerate 50-fold minidystrophin overexpression, but 100-fold overexpression leads to cardiac toxicity.
authors.affiliation: Department of Molecular Microbiology and Immunology, School of Medicine, The University of Missouri , Columbia, Missouri, USA.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Yue
authors.affiliation: Department of Molecular Microbiology and Immunology, School of Medicine, The University of Missouri , Columbia, Missouri, USA.
authors.forename: Nalinda B
authors.initials: NB
authors.lastname: Wasala
authors.affiliation: Department of Molecular Microbiology and Immunology, School of Medicine, The University of Missouri , Columbia, Missouri, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Bostick
authors.affiliation: Department of Molecular Microbiology and Immunology, School of Medicine, The University of Missouri, Columbia, Missouri, USA; Department of Neurology, School of Medicine, The University of Missouri, Columbia, Missouri, USA; Department of Bioengineering, The University of Missouri, Columbia, Missouri, USA.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Duan