title: Inhibition of AQP1 Hampers Osteosarcoma and Hepatocellular Carcinoma Progression Mediated by Bone Marrow-Derived Mesenchymal Stem Cells.
abstract: The complex cross-talk between tumor cells and their surrounding stromal environment plays a key role in the pathogenesis of cancer. Among several cell types that constitute the tumor stroma, bone marrow-derived mesenchymal stem cells (BM-MSCs) selectively migrate toward the tumor microenvironment and contribute to the active formation of tumor-associated stroma. Therefore, here we elucidate the involvement of BM-MSCs to promote osteosarcoma (OS) and hepatocellular carcinoma (HCC) cells migration and invasion and deepening the role of specific pathways. We analyzed the function of aquaporin 1 (AQP1), a water channel known to promote metastasis and neoangiogenes. AQP1 protein levels were analyzed in OS (U2OS) and HCC (SNU-398) cells exposed to conditioned medium from BM-MSCs. Tumor cell migration and invasion in response to BM-MSC conditioned medium were evaluated through a wound healing assay and Boyden chamber, respectively. The results showed that the AQP1 level was increased in both tumor cell lines after treatment with BM-MSC conditioned medium. Moreover, BM-MSCs-mediated tumor cell migration and invasion were hampered after treatment with AQP1 inhibitor. These data suggest that the recruitment of human BM-MSCs into the tumor microenvironment might cause OS and HCC cell migration and invasion through involvement of AQP1.
authors.affiliation: Dipartimento di Scienze Biomediche Avanzate, Universit&#224; degli Studi di Napoli "Federico II", Via Pansini No. 5, 80131 Napoli, Italy. alpelaga@unina.it.|Istituto di Biostrutture e Bioimmagini-CNR, Via De Amicis No. 95, 80145 Napoli, Italy. alpelaga@unina.it.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pelagalli
authors.affiliation: Istituto di Biostrutture e Bioimmagini-CNR, Via De Amicis No. 95, 80145 Napoli, Italy. anna.nardelli@ibb.cnr.it.
authors.forename: Anna
authors.initials: A
authors.lastname: Nardelli
authors.affiliation: Istituto di Biostrutture e Bioimmagini-CNR, Via De Amicis No. 95, 80145 Napoli, Italy. raffaela.fontanella@alice.it.
authors.forename: Raffaela
authors.initials: R
authors.lastname: Fontanella
authors.affiliation: Istituto di Biostrutture e Bioimmagini-CNR, Via De Amicis No. 95, 80145 Napoli, Italy. antonella.zannetti@cnr.it.
authors.forename: Antonella
authors.initials: A
authors.lastname: Zannetti
mesh_terms: D051398:Aquaporin 1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology*; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology; D019789:Tetraethylammonium / Q000494:pharmacology; D059016:Tumor Microenvironment / Q000187:drug effects
chemical_list: D017077:Culture Media, Conditioned; D051398:Aquaporin 1; D019789:Tetraethylammonium
keywords: AQP1; BM-MSCs; hepatocellular carcinoma; invasion; migration; osteosarcoma