title: Isolation and Identification of the Antimicrobial Agent Beauvericin from the Endophytic Fusarium oxysporum 5-19 with NMR and ESI-MS/MS.
abstract: Endophytic microbe has been proved to be one of rich sources of bioactive natural products with potential application for new drug and pesticide discovery. One cyclodepsipeptide, beauvericin, was firstly isolated from the fermentation broth of Fusarium oxysporum 5-19 endophytic on Edgeworthia chrysantha Linn. Its chemical structure was unambiguously identified by a combination of spectroscopic methods, such as HRESI-MS and (1)H and (13)C NMR. ESI-MS/MS was successfully used to elucidate the splitting decomposition route of the positive molecule ion of beauvericin. Antimicrobial results showed that this cyclodepsipeptide had inhibitory effect on three human pathogenic microbes, Candida albicans, Escherichia coli, and Staphylococcus aureus. In particular, beauvericin exhibited the strongest antimicrobial activity against S. aureus with MIC values of 3.91 μM, which had similar effect with that of the positive control amoxicillin.
authors.affiliation: School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Huawei
authors.identifier: 0000-0002-2430-3845
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Chuanfen
authors.initials: C
authors.lastname: Ruan
authors.affiliation: Department of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Bai
authors.affiliation: School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Zhu
authors.affiliation: School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002176:Candida albicans / Q000254:growth & development*; D047630:Depsipeptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004926:Escherichia coli / Q000254:growth & development*; D005670:Fusarium / Q000737:chemistry* / Q000254:growth & development; D013211:Staphylococcus aureus / Q000254:growth & development*
chemical_list: D000890:Anti-Infective Agents; D047630:Depsipeptides; C004456:beauvericin