title: Clavibacter michiganensis subsp. capsici subsp. nov., causing bacterial canker disease in pepper.
abstract: Clavibacter michiganensis is a Gram-stain-positive bacterium with eight subspecies. One of these subspecies is C. michiganensis subsp. michiganensis, which causes bacterial canker disease in tomato. Bacterial strains showing very similar canker disease symptoms to those of a strain originally classified as C. michiganensis have been isolated from pepper. In this paper, we reclassified strains isolated from pepper. On the basis of phylogenetic analysis with 16S rRNA gene sequences, the strains isolated from pepper were grouped in a separate clade from other subspecies of C. michiganensis. Biochemical, physiological and genetic characteristics of strain PF008T, which is the representative strain of the isolates from pepper, were examined in this study. Based on multi-locus sequence typing and other biochemical and physiological features including colony color, utilization of carbon sources and enzyme activities, strain PF008T was categorically differentiated from eight subspecies of C. michiganensis. Moreover, genome analysis showed that the DNA G+C content of strain PF008T is 73.2 %. These results indicate that PF008T is distinct from other known subspecies of C. michiganensis. Therefore, we propose a novel subspecies, C. michiganensis subsp. capsici, causing bacterial canker disease in pepper, with a type strain of PF008T (=KACC 18448T=LMG 29047T).
authors.affiliation: Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, South Korea.
authors.forename: Eom-Ji
authors.initials: EJ
authors.lastname: Oh
authors.affiliation: Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, South Korea.
authors.forename: Chungyun
authors.initials: C
authors.lastname: Bae
authors.affiliation: Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, South Korea.
authors.forename: Han-Beoyl
authors.initials: HB
authors.lastname: Lee
authors.affiliation: Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, South Korea.
authors.forename: In Sun
authors.initials: IS
authors.lastname: Hwang
authors.affiliation: National Plant Quarantine Service, Gimcheon 39660, South Korea.
authors.forename: Hyok-In
authors.initials: HI
authors.lastname: Lee
authors.affiliation: National Plant Quarantine Service, Gimcheon 39660, South Korea.
authors.forename: Mi Chi
authors.initials: MC
authors.lastname: Yea
authors.affiliation: National Plant Quarantine Service, Gimcheon 39660, South Korea.
authors.forename: Kyu-Ock
authors.initials: KO
authors.lastname: Yim
authors.affiliation: Agro Materials Safety Evaluation Division, National Academy of Agricultural Science, Rural Development Administration, Wanju 55365, South Korea.
authors.forename: Seungdon
authors.initials: S
authors.lastname: Lee
authors.affiliation: Crop Cultivation & Environment Research Division, National Institute of Crop Science, Rural Development Administration, Suwon 16429, South Korea.
authors.forename: Sunggi
authors.initials: S
authors.lastname: Heu
authors.affiliation: Department of Plant Medicine, Chungbuk National University, Cheongju 28644, South Korea.
authors.forename: Jae-Soon
authors.initials: JS
authors.lastname: Cha
authors.affiliation: Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, South Korea.
authors.forename: Chang-Sik
authors.initials: CS
authors.lastname: Oh
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002212:Capsicum / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S