title: Functional synergy of anti-mir221 and nanohydroxyapatite scaffold in bone tissue engineering of rat skull.
abstract: An appropriate cell source, effective cell modification, and proper supportive matrices are the main bases of tissue engineering. The effectiveness of anti-mir221 or hydroxyapatite (HA) in improving the osteogenic differentiation of mesenchymal stem cells (MSCs) has been reported previously. Herein, simultaneous application of these osteogenic inducers was investigated in vivo. The Poly-caprolactone (PCL)/HA nanofibers were characterized using contact angle measurement, tensile test, Fourier transform infrared spectroscopy, and electron microscopy. Rat MSCs were isolated, characterized and transfected with anti-mir221. The rats were divided into 4 groups and an 8 mm defect were created in the mid-calvaria of each rat by trephine bur. Group 1 received (PCL)/HA nanofibers, group 2 received (PCL)/HA nanofibers plus autologous MSCs, group 3 received (PCL)/HA nanofibers plus MSCs transfected with anti-mir221, and group 4 rats were left empty as an additional control group. Histomorphometric and radiomorphometric evaluation after 4 and 8 weeks revealed more new bone formation in the cell-treated groups compared to the scaffold alone group. There was evidence for a combination of increased osteoclasts and osteoblast vascular lake containing red blood cells in the anti-mir221 transfected group. New bone penetration into the scaffolds empirically demonstrated the capability of this combination for efficient osteointegration. Altogether, the co-application of HA and anti-mir221 transfected cells can enhance bone healing of the rat skull.
authors.affiliation: Kish International Campus, University of Tehran, Tehran, Iran.
authors.forename: Mahya
authors.initials: M
authors.lastname: Sadeghi
authors.affiliation: Department of Biotechnology, College of Science, University of Tehran, P.O. Box: 14155-6455, Tehran, Iran. b.bakhshandeh@ut.ac.ir.
authors.forename: Behnaz
authors.initials: B
authors.lastname: Bakhshandeh
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Dehghan
authors.affiliation: School of Chemical Engineering, University College of Engineering, University of Tehran, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Mehrnia
authors.affiliation: Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Khojasteh
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D049329:Nanostructures / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry; D051381:Rats; D012886:Skull / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014057:Tomography, X-Ray Computed
chemical_list: C576526:MIRN221 microRNA, rat; D035683:MicroRNAs; D011091:Polyesters; D017886:Durapatite