title: [Fibrinogen/fibrin-specific enzymes from copperhead (Agkistrodon halys halys) and cobra (Naja oxiana eichwald) snake venoms].
abstract: Ability of fractions of cobra's (Naja oxiana Eichwald) and copperhead snake's (Agkistrodon halys halys) venoms to hydrolyze fibrinogen/fibrin was studied. In cobra's snake a component with molecular mass of nearly 60 kDa was found to hydrolyze a-chain of fibrinogen but failed to hydrolyze casein/azocasein and fibrin. A fibrinogen-specific metalloproteinase, the enzyme was inhibited by EDTA. Cobra's venom reduced the mass of donor's fresh blood clots. The copperhead snake's venom and the fractions obtained by gel-filtration (HW-50) and ion exchange chromatography (DEAE-650) were found to hydrolyze casein/azocasein, a- and b-chains of fibrinogen/fibrin and donor's blood clots. The results from the study of the venom and proteolytically active fractions are the evidence for a thrombolytic potential in a copperhead snake's venom.
authors.affiliation: Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences, Tashkent, Republic of Uzbekistan.
authors.forename: E S
authors.initials: ES
authors.lastname: Yunusova
authors.affiliation: Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences, Tashkent, Republic of Uzbekistan.
authors.forename: E S
authors.initials: ES
authors.lastname: Sadykov
authors.affiliation: Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences, Tashkent, Republic of Uzbekistan.
authors.forename: N M
authors.initials: NM
authors.lastname: Sultanalieva
authors.affiliation: Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences, Tashkent, Republic of Uzbekistan.
authors.forename: A V
authors.initials: AV
authors.lastname: Shkinev
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D002364:Caseins / Q000737:chemistry; D004546:Elapid Venoms / Q000737:chemistry / Q000201:enzymology / Q000494:pharmacology*; D010450:Endopeptidases / Q000032:analysis / Q000494:pharmacology; D005337:Fibrin / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D014757:Viper Venoms / Q000737:chemistry / Q000201:enzymology / Q000494:pharmacology*
chemical_list: D002364:Caseins; D004546:Elapid Venoms; D014757:Viper Venoms; D005337:Fibrin; D010450:Endopeptidases
keywords: chromatography; fibrin(ogen)ases; proteinases; snake venoms; thrombolytic activity