title: Protection of human γB-crystallin from UV-induced damage by epigallocatechin gallate: spectroscopic and docking studies.
abstract: The transparency of the human eye lens depends on the solubility and stability of the structural proteins of the eye lens, the crystallins. Although the mechanism of cataract formation is still unclear, it is believed to involve protein misfolding and/or aggregation of proteins due to the influence of several external factors such as ultraviolet (UV) radiation, low pH, temperature and exposure to chemical agents. In this article, we report the study of UV induced photo-damage (under oxidative stress) of recombinant human γB-crystallin in vitro in the presence of the major green tea polyphenol, (-)-epigallocatechin gallate (EGCG). We have shown that EGCG has the ability to protect human γB-crystallin from oxidative stress-induced photo-damage.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. swagata@chem.iitkgp.ernet.in.
authors.forename: Susmitnarayan
authors.initials: S
authors.lastname: Chaudhury
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. swagata@chem.iitkgp.ernet.in.
authors.forename: Sudipta
authors.initials: S
authors.lastname: Bag
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Madhuparna
authors.initials: M
authors.lastname: Bose
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Das
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Ananta Kumar
authors.initials: AK
authors.lastname: Ghosh
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. swagata@chem.iitkgp.ernet.in.
authors.forename: Swagata
authors.initials: S
authors.lastname: Dasgupta
mesh_terms: D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D015394:Molecular Structure; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011485:Protein Binding; D055550:Protein Stability; D011837:Radiation-Protective Agents / Q000737:chemistry* / Q000494:pharmacology; D012995:Solubility; D013057:Spectrum Analysis; D014466:Ultraviolet Rays / Q000009:adverse effects; D038222:gamma-Crystallins / Q000737:chemistry* / Q000187:drug effects / Q000528:radiation effects
chemical_list: D011837:Radiation-Protective Agents; C107738:crystallin, gammaB; D038222:gamma-Crystallins; D002392:Catechin; C045651:epigallocatechin gallate