title: Lung Altered Expression of IL-1β mRNA and Its Signaling Pathway Molecules in Obese-asthmatic Male Wistar Rats.
abstract: Epidemiological and clinical studies indicate a close relationship between obesity and asthma. Here, we determined the impact of diet-induced obesity on the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA as well as IL-1β protein level and pathological changes in male Wistar rat's lung after sensitization with ovalbumin (OVA). Twenty male Wistar rats divided into four groups, control with normal diet (C+ND), OVA-sensitized with normal diet (S+ND), control with high-fat diet (C+HFD), and OVA-sensitized with high-fat diet (S+HFD). All rats fed for 12 weeks with standard pellets or high-fat diet while sensitization and challenging with OVA or saline were done for groups in the last month. In the end of intervention, lung was isolated and tested for the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA with real time-PCR method, and pathological changes were determined. Diet-induced obesity groups showed increased weight, obesity indexes and lipid profiles The expression levels of IL-1β mRNA in OVA-sensitization groups (S+ND and S+HFD) showed a significant increase compared with other groups. Also in S+HFD group, expression level of IRAK-1 and TRAF-6 mRNA were markedly higher than other groups (p<0.001). The pathological changes were marked in sensitized groups compared to non-sensitized groups; with marked increase in obese sensitized rat. The results showed that high fat diet caused overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA as well as IL-1β protein in an experimental model of asthma. Our results suggest that obese-asthmatic conditions may lead to the local production and activation of pro-inflammatory agents.
authors.affiliation: Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Aslani
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Rana
authors.initials: R
authors.lastname: Keyhanmanesh
authors.affiliation: School of advanced medical sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Amir Mehdi
authors.initials: AM
authors.lastname: Khamaneh
authors.affiliation: Department of Basic Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Ebrahimi Saadatlou
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mehran
authors.initials: M
authors.lastname: Mesgari Abbasi
authors.affiliation: Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Alipour
mesh_terms: D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D004041:Dietary Fats / Q000009:adverse effects / Q000494:pharmacology; D004195:Disease Models, Animal; D053592:Interleukin-1 Receptor-Associated Kinases / Q000276:immunology; D053583:Interleukin-1beta / Q000276:immunology*; D008168:Lung / Q000276:immunology* / Q000473:pathology; D008297:Male; D009765:Obesity / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*; D048029:TNF Receptor-Associated Factor 6 / Q000276:immunology
chemical_list: D004041:Dietary Fats; C000594089:IL1B protein, rat; D053583:Interleukin-1beta; D012333:RNA, Messenger; D048029:TNF Receptor-Associated Factor 6; C547807:IRAK1 protein, rat; D053592:Interleukin-1 Receptor-Associated Kinases
keywords: Asthma; IL-1β; IRAK-1; Lung; Obesity; TRAF-6