title: Emerging concepts for the in vitro derivation of murine haematopoietic stem and progenitor cells.
abstract: Well into the second decade of the 21st century, the field of regenerative medicine is bursting with hopes and promises to heal young and old. The bespoken generation of cells is thought to offer unprecedented cures for a vast range of diseases. Haematological disorders have already benefited tremendously from stem cell therapy in the form of bone marrow transplantation. However, lack of compatible donors often means that patients remain on transplantation waiting lists for too long. The in vitro derivation of haematopoietic stem cells offers the possibility to generate tailor-made cells for the treatment of these patients. Promising approaches to generate in vitro-derived blood progenitors include the directed differentiation of pluripotent stem cells and the reprogramming of somatic cells.
authors.affiliation: Cancer Research UK Stem Cell Haematopoiesis Group, Cancer Research UK Manchester Institute, The University of Manchester, UK.
authors.forename: Eva
authors.initials: E
authors.lastname: Garcia-Alegria
authors.affiliation: Cancer Research UK Stem Cell Haematopoiesis Group, Cancer Research UK Manchester Institute, The University of Manchester, UK.
authors.forename: Sara
authors.initials: S
authors.lastname: Menegatti
authors.affiliation: Cancer Research UK Stem Cell Biology Group, Cancer Research UK Manchester Institute, The University of Manchester, UK.
authors.forename: Kiran
authors.initials: K
authors.lastname: Batta
authors.affiliation: Cancer Research UK Stem Cell Haematopoiesis Group, Cancer Research UK Manchester Institute, The University of Manchester, UK.
authors.forename: Sara
authors.initials: S
authors.lastname: Cuvertino
authors.affiliation: Cancer Research UK Stem Cell Biology Group, Cancer Research UK Manchester Institute, The University of Manchester, UK.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Florkowska
authors.affiliation: Cancer Research UK Stem Cell Haematopoiesis Group, Cancer Research UK Manchester Institute, The University of Manchester, UK.
authors.forename: Valerie
authors.initials: V
authors.lastname: Kouskoff
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation / Q000235:genetics*; D065150:Cellular Reprogramming / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D051379:Mice; D044968:Regenerative Medicine; D013234:Stem Cells / Q000166:cytology*
keywords: bone marrow transplantation; embryonic stem cells; hemogenic endothelium; reprogramming