title: Rapid Screening of Bovine Milk Oligosaccharides in a Whey Permeate Product and Domestic Animal Milks by Accurate Mass Database and Tandem Mass Spectral Library.
abstract: A bovine milk oligosaccharide (BMO) library, prepared from cow colostrum, with 34 structures was generated and used to rapidly screen oligosaccharides in domestic animal milks and a whey permeate powder. The novel library was entered into a custom Personal Compound Database and Library (PCDL) and included accurate mass, retention time, and tandem mass spectra. Oligosaccharides in minute-sized samples were separated using nanoliquid chromatography (nanoLC) coupled to a high resolution and sensitive quadrupole-Time of Flight (Q-ToF) MS system. Using the PCDL, 18 oligosaccharides were found in a BMO-enriched product obtained from whey permeate processing. The usefulness of the analytical system and BMO library was further validated using milks from domestic sheep and buffaloes. Through BMO PCDL searching, 15 and 13 oligosaccharides in the BMO library were assigned in sheep and buffalo milks, respectively, thus demonstrating significant overlap between oligosaccharides in bovine (cow and buffalo) and ovine (sheep) milks. This method was shown to be an efficient, reliable, and rapid tool to identify oligosaccharide structures using automated spectral matching.
authors.affiliation: Department of Food Science and Technology, University of California-Davis , Davis, California 95616, United States.
authors.forename: Hyeyoung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Agilent Technologies, Inc. , Santa Clara, California 95051, United States.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Cuthbertson
authors.affiliation: School of Chemical Sciences, University of Auckland , Auckland 1142, New Zealand.
authors.forename: Don E
authors.initials: DE
authors.lastname: Otter
authors.affiliation: Department of Food Science and Technology, University of California-Davis , Davis, California 95616, United States.|Foods for Health Institute, University of California-Davis , Davis, California 95616, United States.
authors.forename: Daniela
authors.initials: D
authors.lastname: Barile
mesh_terms: D000818:Animals; D002020:Buffaloes; D002417:Cattle; D003126:Colostrum / Q000737:chemistry; D016208:Databases, Factual; D008892:Milk / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D012756:Sheep; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000067796:Whey / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides
keywords: bovine milk oligosaccharides; buffalo milk; cow milk; sheep milk; tandem mass spectral library; whey permeate