title: Fungal keratitis due to Schizophyllum commune: an emerging pathogenic fungus.
abstract: Fungal keratitis due to Schizophyllum commune is very rare. In this study, we report the clinical and microbiological profile of five patients with fungal keratitis due to S. commune. Direct microscopic examination of corneal scrapings from all five patients showed septate branching hyaline fungal filaments. Similarly, in all five patients Sabouraud dextrose agar (SDA) plates inoculated with corneal scrapings showed white, cottony colonies on the second day of incubation. Lactophenol cotton blue stained wet preparation of 7-day-old colonies on SDA revealed clamp connections and no spores. The fungus was identified by its characteristic clamp connections, fan-shaped bracket fruiting body with pinkish-grey longitudinally split-radiating gills. The phenotypic identification of one of the five isolates further conformed by ITS sequencing. Treatment outcome was available for two of the five patients; in these two patients, the keratitis resolved with topical natamycin.
authors.affiliation: Apollo Institute of Medical Sciences and Research, Hyderabad, India.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Reddy
authors.affiliation: GHR Micro Diagnostics, Hyderabad, India.
authors.forename: Rangaiahgari
authors.initials: R
authors.lastname: Ashok
authors.affiliation: Vasan Eye Care, Hyderabad, India.
authors.forename: Madhavi
authors.initials: M
authors.lastname: Majety
authors.affiliation: Medivision Eye and Health Care Centre, Hyderabad, India.
authors.forename: Megharaj
authors.initials: M
authors.lastname: Chitta
authors.affiliation: Win Vision Eye Hospitals, Hyderabad, India.
authors.forename: Nitesh
authors.initials: N
authors.lastname: Narayen
mesh_terms: D000328:Adult; D000935:Antifungal Agents / Q000008:administration & dosage; D003315:Cornea / Q000382:microbiology; D015821:Eye Infections, Fungal / Q000188:drug therapy / Q000382:microbiology*; D005260:Female; D006801:Humans; D007634:Keratitis / Q000188:drug therapy / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D010866:Natamycin / Q000008:administration & dosage; D012189:Retrospective Studies; D012567:Schizophyllum / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D000935:Antifungal Agents; D010866:Natamycin
keywords: 
  Schizophyllum commune
  ; Basidiomycota; Keratitis; basidiocarp; natamycin; phenotypic identification