title: Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016.
abstract: We identified a novel plasmid-mediated colistin-resistance gene in porcine and bovine colistin-resistant Escherichia coli that did not contain mcr-1. The gene, termed mcr-2, a 1,617 bp phosphoethanolamine transferase harboured on an IncX4 plasmid, has 76.7% nucleotide identity to mcr-1. Prevalence of mcr-2 in porcine colistin-resistant E. coli (11/53) in Belgium was higher than that of mcr-1 (7/53). These data call for an immediate introduction of mcr-2 screening in ongoing molecular epidemiological surveillance of colistin-resistant Gram-negative pathogens.
authors.affiliation: Laboratory of Medical Microbiology, Wilrijk, Belgium.
authors.forename: Basil Britto
authors.initials: BB
authors.lastname: Xavier
authors.forename: Christine
authors.initials: C
authors.lastname: Lammens
authors.forename: Rohit
authors.initials: R
authors.lastname: Ruhal
authors.forename: Samir
authors.initials: S
authors.lastname: Kumar-Singh
authors.forename: Patrick
authors.initials: P
authors.lastname: Butaye
authors.forename: Herman
authors.initials: H
authors.lastname: Goossens
authors.forename: Surbhi
authors.initials: S
authors.lastname: Malhotra-Kumar
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001426:Bacterial Proteins / Q000235:genetics*; D002417:Cattle / Q000382:microbiology*; D003091:Colistin / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D013552:Swine / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003091:Colistin
keywords: Belgium; Escherichia coli; IncX4; colistin resistance; mcr-1; mcr-2; phosphoethanolamine transferase; pigs