title: Effect of Lipid Partitioning on Predictions of Acute Toxicity of Oil Sands Process Affected Water to Embryos of Fathead Minnow (Pimephales promelas).
abstract: Dissolved organic compounds in oil sands process affected water (OSPW) are known to be responsible for most of its toxicity to aquatic organisms, but the complexity of this mixture prevents use of traditional bottom-up approaches for predicting toxicities of mixtures. Therefore, a top-down approach to predict toxicity of the dissolved organic fraction of OSPW was developed and tested. Accurate masses (i.e., m/z) determined by ultrahigh resolution mass spectrometry in negative and positive ionization modes were used to assign empirical chemical formulas to each chemical species in the mixture. For each chemical species, a predictive measure of lipid accumulation was estimated by stir-bar sorptive extraction (SBSE) to poly(dimethyl)siloxane, or by partitioning to solid-supported lipid membranes (SSLM). A narcosis mode of action was assumed and the target-lipid model was used to estimate potencies of mixtures by assuming strict additivity. A model developed using a combination of the SBSE and SSLM lipid partitioning estimates, whereby the accumulation of chemicals to neutral and polar lipids was explicitly considered, was best for predicting empirical values of LC50 in 96-h acute toxicity tests with embryos of fathead minnow (Pimephales promelas). Model predictions were within 4-fold of observed toxicity for 75% of OSPW samples, and within 8.5-fold for all samples tested, which is comparable to the range of interlaboratory variability for in vivo toxicity testing.
authors.affiliation: Toxicology Centre, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5A2, Canada.
authors.forename: Garrett D
authors.initials: GD
authors.lastname: Morandi
authors.affiliation: Division of Analytical and Environmental Toxicology, University of Alberta , Edmonton, Alberta Canada.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Toxicology Centre, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5A2, Canada.
authors.forename: Steve B
authors.initials: SB
authors.lastname: Wiseman
authors.affiliation: Division of Analytical and Environmental Toxicology, University of Alberta , Edmonton, Alberta Canada.
authors.forename: Alberto Dos Santos
authors.initials: Ados S
authors.lastname: Pereira
authors.affiliation: Division of Analytical and Environmental Toxicology, University of Alberta , Edmonton, Alberta Canada.
authors.forename: Jonathan W
authors.initials: JW
authors.lastname: Martin
authors.affiliation: Toxicology Centre, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5A2, Canada.|Department of Veterinary Biomedical Sciences, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5A2, Canada.|Department of Zoology, and Center for Integrative Toxicology, Michigan State University , East Lansing, Michigan 48823, United States.|School of Biological Sciences, University of Hong Kong , Hong Kong, SAR China.|State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, People's Republic of China.
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
mesh_terms: D000818:Animals; D003530:Cyprinidae; D008055:Lipids; D061329:Oil and Gas Fields; D014867:Water; D014874:Water Pollutants, Chemical
chemical_list: D008055:Lipids; D014874:Water Pollutants, Chemical; D014867:Water