title: Fluorometric cell-based assay for β-galactosidase activity in probiotic gram-positive bacterial cells - Lactobacillus helveticus.
abstract: Although methods for measuring β-galactosidase activity in intact gram-negative bacterial cells have been reported, the methods may not be applicable to measuring β-galactosidase activity in gram-positive bacterial cells. This report focuses on the development of a fluorometric cell-based assay for measuring β-galactosidase activity in gram-positive cells.
authors.affiliation: Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, 410 Patricia A. Sullivan Science Building, 301 McIver Street, Greensboro, NC 27412, United States.
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Watson
authors.affiliation: Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, 410 Patricia A. Sullivan Science Building, 301 McIver Street, Greensboro, NC 27412, United States. Electronic address: prof.chiu@gmail.com.
authors.forename: Norman H L
authors.initials: NHL
authors.lastname: Chiu
mesh_terms: D005470:Fluorometry; D052198:Lactobacillus helveticus / Q000201:enzymology*; D019936:Probiotics; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D001616:beta-Galactosidase
keywords: Fluorescence; Gram-positive; β-galactosidase