title: Utilization of Chromogenic In Situ Hybridization to Assess Ploidy in the Diagnosis of Hydatidiform Mole.
abstract: OBJECTIVE
  Ploidy assessment is often required for the diagnosis of partial molar pregnancy. While fluorescence in situ hybridization has been shown to be effective, it is not available in many laboratories. We validated chromogenic in situ hybridization (CISH) for this purpose.
  
  
  METHODS
  CISH using probes to chromosomes 17 and 10 was performed on 20 POC cases with known cytogenetics to establish a reference percentage. This was then used to classify a randomized set of abnormal and normal cases.
  
  
  RESULTS
  An abnormal CISH cutoff of greater than 7% was established. All abnormal cases (six triploid and three tetraploid), 11 "normal" (46, XX or XY or undetectable abnormalities), and one trisomy 10 were all correctly classified by the assay.
  
  
  CONCLUSIONS
  CISH is a useful ancillary technique for the diagnosis of molar pregnancy. Its greater accessibility and ability to score even rare placental tissue in a background of maternal tissue offer advantages over other methods.
authors.affiliation: From the Department of Pathology, Torrance Memorial Medical Center, Torrance, CA.
authors.forename: John P
authors.initials: JP
authors.lastname: Kunesh
authors.affiliation: From the Department of Pathology, Torrance Memorial Medical Center, Torrance, CA.
authors.forename: Jacqueline G
authors.initials: JG
authors.lastname: Kunesh
authors.affiliation: From the Department of Pathology, Torrance Memorial Medical Center, Torrance, CA.
authors.forename: Rachel Jade
authors.initials: RJ
authors.lastname: Jorgensen
authors.affiliation: From the Department of Pathology, Torrance Memorial Medical Center, Torrance, CA.
authors.forename: Catrina D
authors.initials: CD
authors.lastname: Corral
authors.affiliation: From the Department of Pathology, Torrance Memorial Medical Center, Torrance, CA.
authors.forename: John D
authors.initials: JD
authors.lastname: Blakey
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000175:diagnosis* / Q000235:genetics; D017403:In Situ Hybridization / Q000379:methods*; D011003:Ploidies; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000175:diagnosis* / Q000235:genetics
keywords: CISH; Gynecological; Hydatidiform mole; In situ hybridization; Molar pregnancy; Molecular diagnostics; Obstetric and perinatal; Partial mole