title: Marker-aided Incorporation of Xa38, a Novel Bacterial Blight Resistance Gene, in PB1121 and Comparison of its Resistance Spectrum with xa13 + Xa21.
abstract: Basmati rice is preferred internationally because of its appealing taste, mouth feel and aroma. Pusa Basmati 1121 (PB1121) is a widely grown variety known for its excellent grain and cooking quality in the international and domestic market. It contributes approximately USD 3 billion to India's forex earning annually by being the most traded variety. However, PB1121 is highly susceptible to bacterial blight (BB) disease. A novel BB resistance gene Xa38 was incorporated in PB1121 from donor parent PR114-Xa38 using a modified marker-assisted backcross breeding (MABB) scheme. Phenotypic selection prior to background selection was instrumental in identifying the novel recombinants with maximum recovery of recurrent parent phenome. The strategy was effective in delimiting the linkage drag to <0.5 mb upstream and <1.9 mb downstream of Xa38 with recurrent parent genome recovery upto 96.9% in the developed NILs. The NILs of PB1121 carrying Xa38 were compared with PB1121 NILs carrying xa13 + Xa21 (developed earlier in our lab) for their resistance to BB. Both NILs showed resistance against the Xoo races 1, 2, 3 and 6. Additionally, Xa38 also resisted Xoo race 5 to which xa13 + Xa21 was susceptible. The PB1121 NILs carrying Xa38 gene will provide effective control of BB in the Basmati growing region.
authors.affiliation: Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Ranjith K
authors.initials: RK
authors.lastname: Ellur
authors.affiliation: Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Apurva
authors.initials: A
authors.lastname: Khanna
authors.affiliation: Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Gopala Krishnan
authors.initials: GK
authors.lastname: S
authors.affiliation: Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Prolay K
authors.initials: PK
authors.lastname: Bhowmick
authors.affiliation: RBGRC, ICAR-Indian Agricultural Research Institute, Aduthurai, Tamil Nadu, India.
authors.forename: K K
authors.initials: KK
authors.lastname: Vinod
authors.affiliation: RBGRC, ICAR-Indian Agricultural Research Institute, Aduthurai, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Nagarajan
authors.affiliation: Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Kalyan K
authors.initials: KK
authors.lastname: Mondal
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology, New Delhi, India.
authors.forename: Nagendra K
authors.initials: NK
authors.lastname: Singh
authors.affiliation: School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India.
authors.forename: Kuldeep
authors.initials: K
authors.lastname: Singh
authors.affiliation: Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Kumble Vinod
authors.initials: KV
authors.lastname: Prabhu
authors.affiliation: Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Singh
mesh_terms: D001947:Breeding; D060467:Disease Resistance / Q000235:genetics*; D008040:Genetic Linkage; D007194:India; D012275:Oryza / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology / Q000517:prevention & control; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology; D014974:Xanthomonas / Q000235:genetics / Q000472:pathogenicity
chemical_list: D010940:Plant Proteins