title: Knockdown of Immature Colon Carcinoma Transcript 1 Inhibits Proliferation and Promotes Apoptosis of Non-Small Cell Lung Cancer Cells.
abstract: Non-small cell lung cancer, as the most frequent type lung cancer, has lower survival rate of 5 years, despite improvements in surgery and chemotherapy. Previous studies showed immature colon carcinoma transcript 1 is closely related to tumorigenesis of human cancer cells. In the present study, we found immature colon carcinoma transcript 1 was overexpressed in lung cancer tissues using Oncomine database mining, and the biological effect of immature colon carcinoma transcript 1 was investigated in non-small cell lung cancer cell lines 95D and A549. Lentivirus-mediated RNA interference was used to knock down immature colon carcinoma transcript 1 expression in 95D and A549 cells in vitro, and the knockdown efficiency was determined using quantitative real-time polymerase chain reaction and Western blot assay. Knockdown of immature colon carcinoma transcript 1 significantly suppressed non-small cell lung cancer cell proliferation and colony formation ability confirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assay. Flow cytometry was applied to measure cell cycle arrest, and the result showed the cell cycle arrested in G2/M phase in 95D cells and arrested in G0/G1phase in A549 cells. Furthermore, we measured the levels of cell cycle-associated proteins by Western blot analysis and found immature colon carcinoma transcript 1 -mediated cell proliferation inhibition appeared due to downregulation of cell cycle activator cyclin D1 and upregulation of cell cycle inhibitor p21. In addition, immature colon carcinoma transcript 1 silencing significantly induced non-small cell lung cancer cell apoptosis by annexin V/7-amino-actinomycin D double-staining assay. All our data suggest that immature colon carcinoma transcript 1 may play an important role for non-small cell lung cancer cell proliferation and could be a potential molecular target for diagnosing and treating human non-small cell lung cancer.
authors.affiliation: 1 Department of Thoracic Surgery, Shanghai Tenth People's Hospital, Shanghai, China.
authors.forename: Yiling
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 1 Department of Thoracic Surgery, Shanghai Tenth People's Hospital, Shanghai, China.
authors.forename: Jiantao
authors.initials: J
authors.lastname: He
authors.affiliation: 1 Department of Thoracic Surgery, Shanghai Tenth People's Hospital, Shanghai, China.
authors.forename: Shenghui
authors.initials: S
authors.lastname: Zhang
authors.affiliation: 1 Department of Thoracic Surgery, Shanghai Tenth People's Hospital, Shanghai, China.
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Yang
authors.affiliation: 1 Department of Thoracic Surgery, Shanghai Tenth People's Hospital, Shanghai, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: 1 Department of Thoracic Surgery, Shanghai Tenth People's Hospital, Shanghai, China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Liu
authors.affiliation: 1 Department of Thoracic Surgery, Shanghai Tenth People's Hospital, Shanghai, China.
authors.forename: Xintian
authors.initials: X
authors.lastname: Wu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012269:Ribosomal Proteins
chemical_list: D018797:Cell Cycle Proteins; C098021:MRPL58 protein, human; D011506:Proteins; D034741:RNA, Small Interfering; D012269:Ribosomal Proteins
keywords: ICT1; NSCLC; RNAi; cell cycle; cell proliferation