title: Novel fabrication method of the peritoneal dialysis filter using silk fibroin with urease fixation system.
abstract: During the last decade, there has been a great advance in the kidney dialysis system by wearable artificial kidney (WAK) system for end-stage renal disease patients. Uremic solute removal and water regeneration system are the most prerequisite for WAK to work properly. In this study, we designed a filtering membrane system by using immobilized urease silk fibroin filter and evaluated its comparative effectiveness with a PVDF filtering system in peritoneal dialysate regeneration system by urea removal efficacy. We evaluated this membrane's characteristic and performances by conducting SEM-EDX analyze, water-binding abilities and porosity test, removal abilities of urea, cytotoxicity assay and enzyme activity assay. Under the condition for optimization of urease, the percentage removal of urea was about 40% and 60% in 50 mg/dL urea solution by urease immobilized PVDF and silk fibroin scaffolds, respectively. The batch experimental result showed that immobilized filter removed more than 50% of urea in 50 mg/dL urea solution. In addition silk fibroin with urease filter removed 90 percent of urea in the peritoneal dialysate after 24 h filtration. We suggest that silk fibroin with urease fixation filter can be used more effectively for peritoneal dialysate regeneration system, which have hydrophilic property and prolonged enzyme activity. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2136-2144, 2017.
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Bo Mi
authors.initials: BM
authors.lastname: Moon
authors.affiliation: Department of Nephrology, Chuncheon Sacred Heart Hospital, Hallym University College of Medicine, Chuncheon, 200-704, Republic of Korea.
authors.forename: Myung-Jin
authors.initials: MJ
authors.lastname: Choi
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Md Tipu
authors.initials: MT
authors.lastname: Sultan
authors.affiliation: Department of Nephrology, Yonsei University Wonju College of Medicine, Wonju, 220-701, Republic of Korea.
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Yang
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Hyung Woo
authors.initials: HW
authors.lastname: Ju
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Ye Ri
authors.initials: YR
authors.lastname: Park
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Soo Hyeon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Min Chae
authors.initials: MC
authors.lastname: Lee
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Ju Yeon
authors.initials: JY
authors.lastname: Jeong
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Ok Joo
authors.initials: OJ
authors.lastname: Lee
authors.affiliation: Department of Materials Science and Engineering College of Information and Electronic Engineering, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Gun Yong
authors.initials: GY
authors.lastname: Sung
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.|Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Hallym University College of Medicine, Chuncheon, 200-704, Republic of Korea.
authors.forename: Chan Hum
authors.initials: CH
authors.lastname: Park
mesh_terms: D000818:Animals; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D010530:Peritoneal Dialysis / Q000295:instrumentation*; D014510:Urease / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005349:Fibroins; D014510:Urease
keywords: bio-filter; peritoneal dialysis; silk fibroin; urea filter; wearable artificial kidney