title: New Sulphated Flavonoids from Tamarix africana and Biological Activities of Its Polar Extract.
abstract: The phytochemical investigation of Tamarix africana Poir. (Tamaricaceae) shoot polar extract afforded three new sulphated flavonoids, (2S,4R)-5,7,4'-trihydroxyflavan-4-ol 5,7-disulphate (1), (2S)-5,7,4'-trihydroxyflavan 7-O-sulphate (2), and (2S)-naringenin 4'-O-sulphate (3), together with ten known compounds. Their structures were determined by spectroscopic methods including 1D and 2D NMR analysis and HRMS. Biological activities of the polar extract ofT. africanashoots related to its phenolic content were also investigated. A high total phenolic content (151.1 mg GAE/g) was found in the methanol shoot extract, which exhibits strong antioxidant activities using the oxygen radical absorbance capacity method and a skin cell-based assay. Moreover, the shoot extract showed significant anti-inflammatory activity, reducing nitric oxide release by 53.5 % at 160 µg/mL in lipopolysaccharide-stimulated RAW 264.7 macrophages. Finally,T. africanashoot extract inhibited the growth of A-549 lung carcinoma cells, with an IC50value of 34 µg/mL.
authors.affiliation: Laboratory of Aromatic and Medicinal Plants, Biotechnology Center at the Technopole of Borj-Cédria (CBBC), Hammam-Lif, Tunisia.
authors.forename: Manel
authors.initials: M
authors.lastname: Karker
authors.affiliation: Dipartimento di Farmacia, Università di Salerno, Fisciano (SA), Italy.
authors.forename: Nunziatina
authors.initials: N
authors.lastname: De Tommasi
authors.affiliation: Laboratory of Extremophile Plants, Biotechnology Center at the Technopole of Borj-Cedria (CBBC), Hammam-Lif, Tunisia.
authors.forename: Abderrazak
authors.initials: A
authors.lastname: Smaoui
authors.affiliation: Laboratory of Extremophile Plants, Biotechnology Center at the Technopole of Borj-Cedria (CBBC), Hammam-Lif, Tunisia.
authors.forename: Chedly
authors.initials: C
authors.lastname: Abdelly
authors.affiliation: Laboratory of Aromatic and Medicinal Plants, Biotechnology Center at the Technopole of Borj-Cédria (CBBC), Hammam-Lif, Tunisia.
authors.forename: Riadh
authors.initials: R
authors.lastname: Ksouri
authors.affiliation: Dipartimento di Farmacia, Università di Pisa, Pisa, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Braca
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013431:Sulfates / Q000737:chemistry; D032363:Tamaricaceae / Q000737:chemistry*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D005419:Flavonoids; D008070:Lipopolysaccharides; D010936:Plant Extracts; D013431:Sulfates; D009569:Nitric Oxide; D000432:Methanol