title: Biocompatibility assessment of nanomaterials for environmental safety screening.
abstract: In view of the extensive use of nanoparticles in countless applications, a fast and effective method for assessing their potential adverse effects on the environment and human health is extremely important. At present, in vitro cell-based assays are the standard approach for screening chemicals for cytotoxicity because of their relative simplicity, sensitivity, and cost-effectiveness compared with animal studies. Regrettably, such cell-based viability assays encounter limitations when applied to determining the biological toxicity of nanomaterials, which often interact with assay components and produce unreliable outcomes. We have established a cell-impedance-based, label-free, real-time cell-monitoring platform suitable for use in a variety of mammalian cell lines that displays results as cell index values. In addition to this real-time screening platform, other traditional cytotoxicity assays were employed to validate cytotoxicity assessments. We suggest that the cell impedance measurement approach is effective and better suited to determining the cytotoxicity of nanomaterials for environmental safety screening. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1170-1182, 2017.
authors.affiliation: Institute of Biomedical Sciences, National Chung Hsing University, Taichung, 40227, Taiwan.
authors.forename: Yi-Hui
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Institute of Biomedical Sciences, National Chung Hsing University, Taichung, 40227, Taiwan.
authors.forename: Show-Mei
authors.initials: SM
authors.lastname: Chuang
authors.affiliation: Institute of Environmental Engineering and Center for Emerging Contaminants Research, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan.
authors.forename: Sheng-Chi
authors.initials: SC
authors.lastname: Huang
authors.affiliation: Institute of Biomedical Sciences, National Chung Hsing University, Taichung, 40227, Taiwan.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Tan
authors.affiliation: Institute of Biomedical Sciences, National Chung Hsing University, Taichung, 40227, Taiwan.
authors.forename: Ruei-Yue
authors.initials: RY
authors.lastname: Liang
authors.affiliation: Institute of Environmental Engineering and Center for Emerging Contaminants Research, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan.
authors.forename: Gordon C C
authors.initials: GC
authors.lastname: Yang
authors.affiliation: Institute of Biomedical Sciences, National Chung Hsing University, Taichung, 40227, Taiwan.|Graduate Institute of Basic Medicine, China Medical University, Taichung, 40402, Taiwan.|Department of Medical Research, China Medical University Hospital, Taichung, 40402, Taiwan.|Department of Biotechnology, Asia University, Taichung, 41354, Taiwan.
authors.forename: Pin Ju
authors.identifier: http://orcid.org/0000-0002-3200-7552
authors.initials: PJ
authors.lastname: Chueh
mesh_terms: D000072283:A549 Cells; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000633:toxicity*; D051379:Mice; D041681:NIH 3T3 Cells; D018384:Oxidative Stress; D010316:Particle Size
keywords: cell-impedance measurement; cytotoxicity; in vitro assays; nanomaterials