title: [Determination of proteomic and metabolic composition of exhaled breath condensate of newborns].
abstract: Here, the possibility of proteomic and metabolomic analysis of the composition of exhaled breath condensate of neonates with respiratory support. The developed method allows non-invasive collecting sufficient amount of the material for identification of disease-specific biomarkers. Samples were collected by using a condensing device that was incorporated into the ventilation system. The collected condensate was analyzed by liquid chromatography coupled with high resolution mass spectrometry and tandem mass spectrometry. The isolated substances were identified with a use of databases for proteins and metabolites. As a result, a number of compounds that compose the exhaled breath condensate was determined and can be considered as possible biomarkers of newborn diseases or stage of development.
authors.affiliation: Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, 117997, Russia.|Moscow Institute of Physics and Technology (State University), Dolgoprudnyi, Moscow oblast, 141700, Russia.|alex.kononikhin@gmail.com.
authors.forename: A S
authors.initials: AS
authors.lastname: Kononikhin
authors.affiliation: Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, 117997, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Chagovets
authors.affiliation: Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, 117997, Russia.|Moscow Institute of Physics and Technology (State University), Dolgoprudnyi, Moscow oblast, 141700, Russia.
authors.forename: N L
authors.initials: NL
authors.lastname: Starodubtseva
authors.affiliation: Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, 117997, Russia.
authors.forename: A Y
authors.initials: AY
authors.lastname: Ryndin
authors.affiliation: Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, 117997, Russia.|Emanuel Institute for Biochemical Physics, Moscow, 119934, Russia.
authors.forename: A E
authors.initials: AE
authors.lastname: Bugrova
authors.affiliation: Moscow Institute of Physics and Technology (State University), Dolgoprudnyi, Moscow oblast, 141700, Russia.|Emanuel Institute for Biochemical Physics, Moscow, 119934, Russia.
authors.forename: Y I
authors.initials: YI
authors.lastname: Kostyukevich
authors.affiliation: Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, 117997, Russia.|Moscow Institute of Physics and Technology (State University), Dolgoprudnyi, Moscow oblast, 141700, Russia.|Emanuel Institute for Biochemical Physics, Moscow, 119934, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Popov
authors.affiliation: Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, 117997, Russia.
authors.forename: V E
authors.initials: VE
authors.lastname: Frankevich
authors.affiliation: Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, 117997, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Ionov
authors.affiliation: Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, 117997, Russia.
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
authors.affiliation: Moscow Institute of Physics and Technology (State University), Dolgoprudnyi, Moscow oblast, 141700, Russia.|Emanuel Institute for Biochemical Physics, Moscow, 119934, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Nikolaev
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002853:Chromatography, Liquid; D045853:Exhalation; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D055442:Metabolome; D020543:Proteome / Q000378:metabolism*; D012121:Respiration, Artificial; D012131:Respiratory Insufficiency / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D020543:Proteome
keywords: exhaled breath condensate (EBC); mass spectrometry; metabolomics; newborns; proteomic analysis