title: Maleic anhydride proton sponge as a novel MALDI matrix for the visualization of small molecules (<250 m/z) in brain tumors by routine MALDI ToF imaging mass spectrometry.
abstract: A novel vacuum stable proton sponge, 4-maleicanhydridoproton sponge (MAPS), was prepared and applied as the matrix in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI) of an aggressive brain tumor tissue (glioblastoma multiforme). Ionic maps of lactate, 2-hydroxyglutarate and chloride anions (m/z 89, 147, 35, respectively) were obtained using a routine MALDI ToF mass spectrometer.
authors.affiliation: Center for Biotechnology and Department for Proteome and Metabolome Research, Faculty of Biology, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld, Germany. kniehaus@cebitec.uni-bielefeld.de.
authors.forename: M
authors.initials: M
authors.lastname: Giampà
authors.forename: M B
authors.initials: MB
authors.lastname: Lissel
authors.forename: T
authors.initials: T
authors.lastname: Patschkowski
authors.forename: J
authors.initials: J
authors.lastname: Fuchser
authors.forename: V H
authors.initials: VH
authors.lastname: Hans
authors.forename: O
authors.initials: O
authors.lastname: Gembruch
authors.forename: H
authors.initials: H
authors.lastname: Bednarz
authors.forename: K
authors.initials: K
authors.lastname: Niehaus
mesh_terms: D001921:Brain / Q000000981:diagnostic imaging*; D001932:Brain Neoplasms / Q000000981:diagnostic imaging*; D002712:Chlorides / Q000032:analysis*; D005909:Glioblastoma / Q000000981:diagnostic imaging*; D005977:Glutarates / Q000032:analysis*; D006801:Humans; D019344:Lactic Acid / Q000032:analysis*; D008299:Maleic Anhydrides / Q000737:chemistry; D011522:Protons; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D002712:Chlorides; D005977:Glutarates; D008299:Maleic Anhydrides; D011522:Protons; C019417:alpha-hydroxyglutarate; D019344:Lactic Acid