title: Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model.
abstract: The systemic administration of autologous bone marrow (BM) derived mononuclear cells (MNCs) is under investigation as a novel therapeutic modality for the treatment of ischemic stroke. Autologous applications raise the possibility that MNCs could potentially be stored as a banked source. There have been no studies that investigate the effects of cryopreservation of BM-MNCs on their functional abilities in stroke models. In the present study, C57BL/6 mice were subjected to middle cerebral artery occlusion (MCAo) for 60 minutes and then divided into two treatment groups: fresh MNCs versus cryopreserved MNCs. BM-MNCs were collected at 22 hours after MCAo and were stored in liquid nitrogen for 12 months in cryopreserved MNCs group. BM-MNCs cellular viability, composition, and phenotype of the various subpopulations of mice BM-MNCs were evaluated by flow cytometry, and the behavioral recovery of stroke animals was tested with freshly harvested MNCs versus cryopreserved MNCs by corner test and ladder rung test. We found that long-term cryopreservation negatively impacts the cellular viability of bone marrow MNCs. Cryopreservation also alters the cellular composition of various subpopulations within the MNCs. However, despite the changes observed in cryopreserved cells, both fresh and frozen MNCs have similar beneficial effect on behavioral and histological outcomes.
authors.affiliation: Stroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.
authors.forename: Bing
authors.identifier: 0000-0001-6860-583X
authors.initials: B
authors.lastname: Yang
authors.affiliation: Stroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.
authors.forename: Kaushik
authors.initials: K
authors.lastname: Parsha
authors.affiliation: Stroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.
authors.forename: Krystal
authors.initials: K
authors.lastname: Schaar
authors.affiliation: Stroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.
authors.forename: Nikunj
authors.identifier: 0000-0003-3908-3731
authors.initials: N
authors.lastname: Satani
authors.affiliation: Stroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.
authors.forename: Xiaopei
authors.initials: X
authors.lastname: Xi
authors.affiliation: Stroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Aronowski
authors.affiliation: Stroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.
authors.forename: Sean I
authors.initials: SI
authors.lastname: Savitz