title: Lysinibacillus endophyticus sp. nov., an indole-3-acetic acid producing endophytic bacterium isolated from corn root (Zea mays cv. Xinken-5).
abstract: A Gram-positive, aerobic, motile, rod-shaped bacterium, designated strain C9(T), was isolated from surface sterilised corn roots (Zea mays cv. Xinken-5) and found to be able to produce indole-3-acetic acid. A polyphasic taxonomic study was carried out to determine the status of strain C9(T). The major cellular fatty acids were found to contain iso-C15:0, anteiso-C15:0 and anteiso-C17:0, and the only menaquinone was identified as MK-7. The polar lipid profile was found to contain diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and an unidentified lipid. The cell wall peptidoglycan was found to be of the A4α L-Lys-D-Asp type and the whole cell sugar was found to be glucose. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain C9(T) belongs to the genus Lysinibacillus and is closely related to Lysinibacillus chungkukjangi NBRC 108948(T) (98.1 % similarity) and Lysinibacillus sinduriensis DSM 27595(T) (98.0 %). However, the low levels of DNA-DNA relatedness and some differential phenotypic characteristics allowed the strain to be distinguished from its close relatives. Therefore, it is concluded that strain C9(T) represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillus endophyticus sp. nov. is proposed. The type strain is C9(T) (=DSM 100506(T) = CGMCC 1.15291(T)).
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, China.|College of Agronomy, Northeast Agricultural University, 150030, Harbin, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, 150030, Harbin, China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Guan
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, 150030, Harbin, China.
authors.forename: Chongxi
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, 150030, Harbin, China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Xiang
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, China. wanggh@iga.ac.cn.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Wang
mesh_terms: D001406:Bacillaceae / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D060026:Endophytes / Q000302:isolation & purification / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D007564:Japan; D058889:Molecular Typing; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D003313:Zea mays / Q000382:microbiology*
chemical_list: D007210:Indoleacetic Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C030737:indoleacetic acid
keywords: 16S rRNA gene; Lysinibacillus endophyticus sp. nov.; Polyphasic taxonomy