title: Evaluation of phytic acid utilization by S. cerevisiae strains used in fermentation processes and biomass production.
abstract: Saccharomyces cerevisiae is a well-studied yeast species used mainly in fermentation processes, bakery, and for SCP (Single Cell Protein) acquisition. The aim of the study was to analyze the possibility of phytic acid utilization as one of the hydrolysis processes carried out by yeast. The analysis of 30 yeast strains used in fermentation and for biomass production, that were grown in media containing phytic acid, revealed a high variability in the biomass production rate and the capability to hydrolyze phytates. No correlation between a high biomass concentration and a high level of phytate hydrolysis was found. Only four analyzed strains (Bayanus IOC Efficience, Sano, PINK EXCEL, FINAROME) were able to reduce the phytic acid concentration by more than 33.5%, from the initial concentration 103.0 ± 2.1 μg/ml to the level below 70 μg/ml. The presented results suggest that the selected wine and fodder yeast can be used as in situ source of phosphohydrolases in fermentation processes, and especially in the production of fodder proteins. However, further studies aimed at the optimization of growing parameters, such as the maximization of phytase secretion, and a comprehensive analysis of the catalytic activity of the isolated phosphohydrolases, are necessary.
authors.affiliation: Department of Biotechnology, Kazimierz Wielki University, Bydgoszcz, Poland.
authors.forename: Dawid
authors.initials: D
authors.lastname: Mikulski
authors.affiliation: Department of Biotechnology, Kazimierz Wielki University, Bydgoszcz, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Kłosowski
mesh_terms: D010832:6-Phytase / Q000378:metabolism; D000821:Animal Feed / Q000382:microbiology; D018533:Biomass; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007218:Industrial Microbiology; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010833:Phytic Acid / Q000096:biosynthesis / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D014920:Wine / Q000382:microbiology
chemical_list: D000431:Ethanol; D010833:Phytic Acid; D010744:Phosphoric Monoester Hydrolases; D010832:6-Phytase
keywords: Hydrolysis of phytates; Phytic acid; S. cerevisiae; Screening