title: Genome-Wide Analysis of the bZIP Gene Family Identifies Two ABI5-Like bZIP Transcription Factors, BrABI5a and BrABI5b, as Positive Modulators of ABA Signalling in Chinese Cabbage.
abstract: bZIP (basic leucine zipper) transcription factors coordinate plant growth and development and control responses to environmental stimuli. The genome of Chinese cabbage (Brassica rapa) encodes 136 putative bZIP transcription factors. The bZIP transcription factors in Brassica rapa (BrbZIP) are classified into 10 subfamilies. Phylogenetic relationship analysis reveals that subfamily A consists of 23 BrbZIPs. Two BrbZIPs within subfamily A, Bra005287 and Bra017251, display high similarity to ABI5 (ABA Insensitive 5). Expression of subfamily A BrbZIPs, like BrABI5a (Bra005287/BrbZIP14) and BrABI5b (Bra017251/BrbZIP13), are significantly induced by the plant hormone ABA. Subcellular localization assay reveal that both BrABI5a and BrABI5b have a nuclear localization. BrABI5a and BrABI5b could directly stimulate ABA Responsive Element-driven HIS (a HIS3 reporter gene, which confers His prototrophy) or LUC (LUCIFERASE) expression in yeast and Arabidopsis protoplast. Deletion of the bZIP motif abolished BrABI5a and BrABI5b transcriptional activity. The ABA insensitive phenotype of Arabidopsis abi5-1 is completely suppressed in transgenic lines expressing BrABI5a or BrABI5b. Overall, these results suggest that ABI5 orthologs, BrABI5a and BrABI5b, have key roles in ABA signalling in Chinese cabbage.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Yili
authors.initials: Y
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Qinhu
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Pei
authors.affiliation: Hybrid Rapeseed Research Centre of Shaanxi Province, Yangling, Shaanxi, 712100, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Aiguang
authors.initials: A
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Huixian
authors.initials: H
authors.lastname: Zhao
authors.affiliation: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Mu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Jingjiang
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.|Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, 47907, United States of America.
authors.forename: Chang Gen
authors.initials: CG
authors.lastname: Xie
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics*; D029741:Brassica rapa / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D055106:Genome-Wide Association Study; D018525:Germination / Q000502:physiology; D007438:Introns / Q000235:genetics; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism*; D030821:Plants, Genetically Modified; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D010937:Plant Growth Regulators; D000040:Abscisic Acid