title: A single center's approach to discriminating donor versus host origin of renal neoplasia in the allograft kidney.
abstract: Renal cell carcinoma (RCC) in the allograft of kidney transplant recipient (KTR) patients is rare and may represent a de novo process arising from the transplanted organ or metastasis from a clinically undetectable host primary. Determination of host versus donor origin is important for staging and management. We report our experience utilizing Penta-C (PC) and Penta-D (PD) short-tandem repeat (STR) microsatellite analysis to discriminate between host and donor origin of RCC identified in renal allografts. We identified 5 KTR patients with RCC in the allograft kidney. The PC and PD microsatellite analysis was applied to tumor, host, and donor formalin-fixed, paraffin-embedded tissue sections and/or fresh blood leukocytes to identify the origin of the neoplastic cells. The PC and PD microsatellite alleles were robustly amplified in all samples. Each case showed one or more informative alleles indicating that the neoplastic cells originate from donor tissue. Allele frequency data indicate that by using both PC and PD markers, we will be able to discriminate between host and donor cell of origin in over 99% of cases. The PC and PD microsatellite analysis is a convenient, robust, and efficient strategy to determine donor versus host origin or RCC in transplant kidney specimens.
authors.affiliation: Department of Pathology, Medical College of Wisconsin, Milwaukee, Wisconsin.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Robin
authors.affiliation: Nephology, Medical College of Wisconsin, Milwaukee, Wisconsin.
authors.forename: Eric P
authors.initials: EP
authors.lastname: Cohen
authors.affiliation: Nephology, Medical College of Wisconsin, Milwaukee, Wisconsin.
authors.forename: Tepsiri
authors.initials: T
authors.lastname: Chongkrairatanakul
authors.affiliation: Nephology, Medical College of Wisconsin, Milwaukee, Wisconsin.
authors.forename: Ehad
authors.initials: E
authors.lastname: Saad
authors.affiliation: Department of Pathology, Medical College of Wisconsin, Milwaukee, Wisconsin. Electronic address: amackinnon@mcw.edu.
authors.forename: A Craig
authors.initials: AC
authors.lastname: Mackinnon
mesh_terms: D000293:Adolescent; D000328:Adult; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology* / Q000628:therapy; D016030:Kidney Transplantation / Q000379:methods; D008297:Male; D018895:Microsatellite Repeats / Q000502:physiology*; D008875:Middle Aged; D014019:Tissue Donors; D014184:Transplantation, Homologous; D055815:Young Adult
keywords: Allograft; Malignancy; Microsatellite analysis; Posttransplant malignancy; Renal cell carcinoma