title: E2 multimeric scaffold for vaccine formulation: immune response by intranasal delivery and transcriptome profile of E2-pulsed dendritic cells.
abstract: The E2 multimeric scaffold represents a powerful delivery system able to elicit robust humoral and cellular immune responses upon systemic administrations. Here recombinant E2 scaffold displaying the third variable loop of HIV-1 Envelope gp120 glycoprotein was administered via mucosa, and the mucosal and systemic immune responses were analysed. To gain further insights into the molecular mechanisms that orchestrate the immune response upon E2 vaccination, we analysed the transcriptome profile of dendritic cells (DCs) exposed to the E2 scaffold with the aim to define a specific gene expression signature for E2-primed immune responses.
  
  
  
  The in vivo immunogenicity and the potential of E2 scaffold as a mucosal vaccine candidate were investigated in BALB/c mice vaccinated via the intranasal route. Fecal and systemic antigen-specific IgA antibodies, cytokine-producing CD4(+) and CD8(+) cells were induced assessing the immunogenicity of E2 particles via intranasal administration. The cytokine analysis identified a mixed T-helper cell response, while the systemic antibody response showed a prevalence of IgG1 isotype indicative of a polarized Th2-type immune response. RNA-Sequencing analysis revealed that E2 scaffold up-regulates in DCs transcriptional regulators of the Th2-polarizing cell response, defining a type 2 DC transcriptomic signature.
  
  
  
  The current study provides experimental evidence to the possible application of E2 scaffold as antigen delivery system for mucosal immunization and taking advantages of genome-wide approach dissects the type of response induced by E2 particles.
authors.affiliation: Institute of Protein Biochemistry, C.N.R, Via Pietro Castellino 111, Naples, 80131, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Trovato
authors.affiliation: Institute of Food Sciences, C.N.R, Via Roma 64, Avellino, 83100, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Maurano
authors.affiliation: Institute of Protein Biochemistry, C.N.R, Via Pietro Castellino 111, Naples, 80131, Italy.
authors.forename: Luciana
authors.initials: L
authors.lastname: D'Apice
authors.affiliation: Institute of Genetics and Biophysics A. Buzzati-Traverso, C.N.R, Via Pietro Castellino 111, Naples, 80131, Italy.
authors.forename: Valerio
authors.initials: V
authors.lastname: Costa
authors.affiliation: Institute of Protein Biochemistry, C.N.R, Via Pietro Castellino 111, Naples, 80131, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Sartorius
authors.affiliation: Institute of Protein Biochemistry, C.N.R, Via Pietro Castellino 111, Naples, 80131, Italy.
authors.forename: Fausta
authors.initials: F
authors.lastname: Cuccaro
authors.affiliation: Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health & Science University, 505 NW 185th Avenue, Beaverton, OR, 97006, USA.
authors.forename: Sean P
authors.initials: SP
authors.lastname: McBurney
authors.affiliation: Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health & Science University, 505 NW 185th Avenue, Beaverton, OR, 97006, USA.
authors.forename: Shelly J
authors.initials: SJ
authors.lastname: Krebs
authors.affiliation: Institute of Genetics and Biophysics A. Buzzati-Traverso, C.N.R, Via Pietro Castellino 111, Naples, 80131, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Prisco
authors.affiliation: Institute of Genetics and Biophysics A. Buzzati-Traverso, C.N.R, Via Pietro Castellino 111, Naples, 80131, Italy.|Department of Science and Technology, University of Naples "Parthenope", Centro Direzionale Site island C4, Naples, 80143, Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Ciccodicola
authors.affiliation: Institute of Food Sciences, C.N.R, Via Roma 64, Avellino, 83100, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Rossi
authors.affiliation: Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health & Science University, 505 NW 185th Avenue, Beaverton, OR, 97006, USA.
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Haigwood
authors.affiliation: Institute of Protein Biochemistry, C.N.R, Via Pietro Castellino 111, Naples, 80131, Italy. p.deberardinis@ibp.cnr.it.
authors.forename: Piergiuseppe
authors.initials: P
authors.lastname: De Berardinis
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D018928:Immunity, Mucosal / Q000276:immunology; D000071497:Immunogenicity, Vaccine; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D059467:Transcriptome; D014612:Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016207:Cytokines; D015699:HIV Envelope Protein gp120; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D014612:Vaccines; C514793:gp120 protein, Human immunodeficiency virus 1
keywords: Bone marrow-derived dendritic cells; E2 scaffold; Mucosal immunity; RNA-Sequencing; Th2; Transcriptome profile; Vaccine