title: Comparison of fluorescent intercalating dyes for quantitative loop-mediated isothermal amplification (qLAMP).
abstract: Real-time or quantitative loop-mediated isothermal amplification (qLAMP) is a promising technique for the accurate detection of pathogens in organisms and the environment. Here we present a comparative study of the performance of six fluorescent intercalating dyes-SYTO-9, SYTO-13, SYTO-82, SYBR Green I, SYBR Gold, EvaGreen-in three different qLAMP model systems. SYTO-9 and SYTO-82, which had the best results, were used for additional enzyme and template titration studies. SYTO-82 demonstrated the best combination of time-to-threshold (Tt) and signal-to-noise ratio (SNR).
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.|Novosibirsk State University, Novosibirsk, Russian Federation.
authors.forename: Igor P
authors.initials: IP
authors.lastname: Oscorbin
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Belousova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Aleksandr I
authors.initials: AI
authors.lastname: Zakabunin
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Ulyana A
authors.initials: UA
authors.lastname: Boyarskikh
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.|Novosibirsk State University, Novosibirsk, Russian Federation.
authors.forename: Maksim L
authors.initials: ML
authors.lastname: Filipenko
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D052160:Benzothiazoles; D004247:DNA / Q000737:chemistry*; D003959:Diamines; D005456:Fluorescent Dyes / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009930:Organic Chemicals; D011804:Quinolines; D059629:Signal-To-Noise Ratio
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D009930:Organic Chemicals; D011804:Quinolines; C461159:SYTO 13; C103389:SYTO 9; C098022:SYBR Green I; D004247:DNA
keywords: Bst DNA polymerase; EvaGreen; LAMP; SYBR Gold; SYBR Green I; SYTO-13; SYTO-82; SYTO-9; isothermal amplification