title: A synthetic C16 omega-hydroxyphytoceramide improves skin barrier functions from diversely perturbed epidermal conditions.
abstract: Omega-hydroxyceramides (ω-OH-Cer) play a crucial role in maintaining the integrity of skin barrier. ω-OH-Cer are the primary lipid constituents of the corneocyte lipid envelope (CLE) covalently attached to the outer surface of the cornified envelope linked to involucrin to become bound form lipids in stratum corneum (SC). CLE becomes a hydrophobic impermeable layer of matured corneocyte preventing loss of natural moisturizing factor inside the corneocytes. More importantly, CLE may also play an important role in the formation of proper orientation of intercellular lipid lamellar structure by interdigitating with the intercellular lipids in a comb-like fashion. Abnormal barrier conditions associated with atopic dermatitis but also UVB-irradiated skins are known to have lowered level of bound lipids, especially ω-OH-Cer, which indicate that ω-OH-Cer play an important role in maintaining the integrity of skin barrier. In this study, protective effects of a novel synthetic C16 omega-hydroxyphytoceramides (ω-OH-phytoceramide) on skin barrier function were investigated. Epidermal barrier disruption was induced by UVB irradiation, tape-stripping in hairless mouse and human skin. Protective effect of damaged epidermis was evaluated using the measurement of transepidermal water loss and cohesion of SC. Increased keratinocyte differentiation was verified using cultured keratinocyte through western blot. Results clearly demonstrated that a synthetic C16 ω-OH-phytoceramide enhanced the integrity of SC and accelerated the recovery of damaged skin barrier function by stimulating differentiation process. In a conclusion, a synthetic C16 ω-OH-phytoceramide treatment improved epidermal homeostasis in several disrupted conditions.
authors.affiliation: Department of Chemical and Biochemical Engineering, Dongguk University, 3-26, Pil-dong, Chung-gu, Seoul, 100-715, Republic of Korea.
authors.forename: Myoung Jin
authors.initials: MJ
authors.lastname: Oh
authors.affiliation: Department of Chemical and Biochemical Engineering, Dongguk University, 3-26, Pil-dong, Chung-gu, Seoul, 100-715, Republic of Korea.
authors.forename: Jin Ju
authors.initials: JJ
authors.lastname: Nam
authors.affiliation: LCS Biotech, Seodun-dong 103-2, Gwonseon-gu, Suwon-si, Gyeonggi-do, 441-857, Republic of Korea.
authors.forename: Eun Ok
authors.initials: EO
authors.lastname: Lee
authors.affiliation: LCS Biotech, Seodun-dong 103-2, Gwonseon-gu, Suwon-si, Gyeonggi-do, 441-857, Republic of Korea.
authors.forename: Jin Wook
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biochemical Engineering, Dongguk University, 3-26, Pil-dong, Chung-gu, Seoul, 100-715, Republic of Korea. dgucsp@dongguk.edu.
authors.forename: Chang Seo
authors.initials: CS
authors.lastname: Park
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002518:Ceramides / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D003876:Dermatitis, Atopic / Q000188:drug therapy*; D004817:Epidermis / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D005260:Female; D006201:Hair Diseases / Q000188:drug therapy*; D006706:Homeostasis / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D008812:Mice, Hairless; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D002518:Ceramides
keywords: Keratinocyte differentiation; Omega-hydroxyceramide; Skin barrier; UV