title: Inhibitory effect of microRNA-27b on interleukin 17 (IL-17)-induced monocyte chemoattractant protein-1 (MCP1) expression.
abstract: We investigated the effect of microRNA-27b (miR-27b), a gene expression regulatory factor, on the expression of monocyte chemoattractant protein-1 (MCP1) stimulated by interleukin 17 (IL-17). After IL-17 had been added to H9C2 cardiomyocytes, an miR-27b mimic was transfected into the H9C2 cells. The mRNA expression levels of miR-27b and MCP1 in the H9C2 cells were detected by SYBR green I fluorescence quantitative reverse transcription polymerase chain reaction. Enzyme-linked immunosorbent assay was used to measure the expression levels of MCP1 protein in the H9C2 cells. The expression of MCP1 mRNA in the H9C2 cells began to increase 2 h after IL-17 stimulation, reached a peak at 4 h, and then decreased. The MCP1 protein level increased gradually in the 24 h following IL-17 stimulation. After transfection with the miR-27b mimic, the expression of miR-27b in the H9C2 cells significantly increased than that in the miRNA negative control group (P < 0.01). The MCP1 mRNA and protein levels in the miR-27b mimic + IL-17 group were significantly reduced than that in the miRNA negative control + IL-17 group (P < 0.01). miR-27b inhibited IL-17-induced MCP1 expression in the H9C2 cells, which demonstrates that treatment with microRNA could alleviate myocardial injury in viral myocarditis.
authors.affiliation: Clinical Laboratory Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: K D
authors.initials: KD
authors.lastname: Huang
authors.affiliation: Clinical Laboratory Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Clinical Laboratory Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: X
authors.initials: X
authors.lastname: Wei
authors.affiliation: Clinical Laboratory Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: F Q
authors.initials: FQ
authors.lastname: Zhang
authors.affiliation: Clinical Laboratory Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Liu
authors.affiliation: Clinical Laboratory Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Ma
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D020381:Interleukin-17 / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D035683:MicroRNAs / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction; D014162:Transfection / Q000379:methods
chemical_list: D018932:Chemokine CCL2; D020381:Interleukin-17; C000594049:MIRN27 microRNA, rat; D035683:MicroRNAs; D012333:RNA, Messenger