title: Fluoride release and uptake abilities of different fissure sealants.
abstract: BACKGROUND
  The long-term capability of resin sealants and glass ionomer cements to release fluoride is associated to a reduction in pit and fissure caries. The regular use of fluoride varnishes/toothpastes can result in the absorption of fluoride into the sealant. The objective of the present study was to assess the fluoride release/uptake capacities of different fissure sealants.
  
  
  METHODS
  Three different fissure sealants (Fuji Triage/GC, Fissurit FX/Voco and Grandio Seal/Voco) were examined. Ten discs of each material were prepared. Each disc was incubated with distilled water and then the solution analyzed for diluted for fluoride concentration, using a combination of fluoride electrode (OrionGP 1 S/N 13824, Orion Research Inc, Boston, MA, USA) connected to an expandable ion analyzer (Orion 720A, Orion Research Inc, Boston, MA, USA). Standard curves between 1 and 100 ppm F- were used to calibrate the electrode. Cumulative fluoride release was measured on days 1, 2, 3, 5, 7, 21, 35 and 49, then two different fluoride varnishes/pastes (Profluorid Varnish/Voco, MI Paste Plus/GC), were applied to the sealants tested, and fluoride release (after reuptake) was measured on days 56, 70 and 84.
  
  
  RESULTS
  Kruskal Wallis test confirmed significant differences in fluoride release between Fuji Triage/GC and Fissurit FX/Voco and Grandio Seal/Voco from day 1 (P < 0.001). The application of fluoride varnish Profluorid Varnish enhanced the fluoride release for all sealants (P < 0.05). MI Paste Plus enhanced the fluoride release for all sealants except for Fuji Triage/GC (P > 0.05).
  
  
  CONCLUSIONS
  The GIC-based sealant (Fuji Triage/GC) released significantly more fluoride than the resin sealants tested. The exposure to the fluoridated varnish (Profluorid Varnish) significantly recharged the sealants tested more than the CPP-ACPF toothpaste (MI Paste Plus).
  
  
  BACKGROUND
  Fissure sealants, fluoride release, fluoride uptake, glass ionomer cements.
authors.affiliation: MD, DDS. Department of Clinical-Surgical, Diagnostic and Pediatric Sciences - Section of Dentistry, University of Pavia, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Poggio
authors.affiliation: DMD, PhD. Department of Clinical-Surgical, Diagnostic and Pediatric Sciences - Section of Dentistry, University of Pavia, Italy.
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Andenna
authors.affiliation: DMD, PhD. Department of Clinical-Surgical, Diagnostic and Pediatric Sciences - Section of Dentistry, University of Pavia, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Ceci
authors.affiliation: DMD, PhD. Department of Brain and Behavioural Sciences - Section of Statistics, University of Pavia, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Beltrami
authors.affiliation: DMD, PhD. Department of Clinical-Surgical, Diagnostic and Pediatric Sciences - Section of Dentistry, University of Pavia, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Colombo
authors.affiliation: MD, DDS. Department of Chemistry, University of Pavia, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Cucca