title: Identification of Novel Vaccine Candidates against Campylobacter through Reverse Vaccinology.
abstract: Campylobacteriosis is the most prevalent bacterial foodborne gastroenteritis affecting humans in the European Union. Human cases are mainly due to Campylobacter jejuni or Campylobacter coli, and contamination is associated with the handling and/or consumption of poultry meat. In fact, poultry constitutes the bacteria's main reservoir. A promising way of decreasing the incidence of campylobacteriosis in humans would be to decrease avian colonization. Poultry vaccination is of potential for this purpose. However, despite many studies, there is currently no vaccine available on the market to reduce the intestinal Campylobacter load in chickens. It is essential to identify and characterize new vaccine antigens. This study applied the reverse vaccinology approach to detect new vaccine candidates. The main criteria used to select immune proteins were localization, antigenicity, and number of B-epitopes. Fourteen proteins were identified as potential vaccine antigens. In vitro and in vivo experiments now need to be performed to validate the immune and protective power of these newly identified antigens.
authors.affiliation: Unit of Viral Genetics and Biosafety (GVB), French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 22440 Ploufragan, France; Unit of Hygiene and Quality of Poultry and Pork Products (HQPAP), French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 22440 Ploufragan, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Meunier
authors.affiliation: Unit of Hygiene and Quality of Poultry and Pork Products (HQPAP), French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 22440 Ploufragan, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Guyard-Nicodème
authors.affiliation: Unit of Viral Genetics and Biosafety (GVB), French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 22440 Ploufragan, France.
authors.forename: Edouard
authors.identifier: 0000-0001-6991-8491
authors.initials: E
authors.lastname: Hirchaud
authors.affiliation: CZ Veterinaria, Porriño, 36400 Pontevedra, Spain.
authors.forename: Alberto
authors.identifier: 0000-0001-9799-7115
authors.initials: A
authors.lastname: Parra
authors.affiliation: Unit of Hygiene and Quality of Poultry and Pork Products (HQPAP), French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 22440 Ploufragan, France.
authors.forename: Marianne
authors.initials: M
authors.lastname: Chemaly
authors.affiliation: Unit of Viral Genetics and Biosafety (GVB), French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 22440 Ploufragan, France.
authors.forename: Daniel
authors.identifier: 0000-0003-0138-7713
authors.initials: D
authors.lastname: Dory
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000737:chemistry / Q000276:immunology*; D002169:Campylobacter Infections / Q000382:microbiology / Q000517:prevention & control* / Q000662:veterinary; D016123:Campylobacter jejuni / Q000737:chemistry / Q000276:immunology*; D002645:Chickens / Q000382:microbiology; D019295:Computational Biology; D003198:Computer Simulation; D055808:Drug Discovery / Q000379:methods; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D006801:Humans; D011201:Poultry Diseases / Q000517:prevention & control*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D018985:Epitopes, B-Lymphocyte