title: Screening, tandem expression and immune activity appraisal of Deinagkistrodon acutus (pit viper) venom mimotopes from a phage display 12-mer peptide library.
abstract: OBJECTIVE
  To design a specific polyclonal antibody against Deinagkistrodon acutus venom (DA-pAb) by immunizating New Zealand white rabbits.
  
  
  RESULTS
  The IgG fraction was purified by affinity chromatography, and specific antibodies were purified by immunoaffinity chromatography. Polyclonal antibodies were subjected to ELISA and western blotting to evaluate their immune reactivity. We identified the mimotopes by screening a phage display 12-mer peptide library against D. acutus venom. After three rounds of biopanning with DA-pAb, 30 positive clones were identified. Eighteen phage clones were sequenced, and their corresponding amino acid sequences were deduced. Additional immunoassays with the peptides and DA-pAb identified five sequences as possible epitopes. Recombinant antigens synthesized with the five epitopes were used for the immunization of BALB/c mice.
  
  
  CONCLUSIONS
  The antibodies induced by these peptides recognized the recombinant antigen and D. acutus venom and protected mice against the hemorrhagic effects of the venom.
authors.affiliation: Department of Emergency, Southwest Hospital, Third Military Medical University, Chongqing, 400038, People's Republic of China.
authors.forename: Guoning
authors.initials: G
authors.lastname: Guo
authors.affiliation: Department of Emergency, Southwest Hospital, Third Military Medical University, Chongqing, 400038, People's Republic of China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Health Management, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, People's Republic of China.
authors.forename: Guoyan
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Department of Emergency, Southwest Hospital, Third Military Medical University, Chongqing, 400038, People's Republic of China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Department of Emergency, Southwest Hospital, Third Military Medical University, Chongqing, 400038, People's Republic of China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Gou
authors.affiliation: Department of Emergency, Southwest Hospital, Third Military Medical University, Chongqing, 400038, People's Republic of China.
authors.forename: Cong
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Emergency, Southwest Hospital, Third Military Medical University, Chongqing, 400038, People's Republic of China. minghua_liu111@126.com.
authors.forename: Minghua
authors.initials: M
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D003435:Crotalid Venoms / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D051379:Mice; D019151:Peptide Library; D011817:Rabbits; D020539:Sequence Analysis, Protein; D017819:Viperidae / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D003435:Crotalid Venoms; D000939:Epitopes; D007074:Immunoglobulin G; D019151:Peptide Library
keywords: Deinagkistrodon acutus; Mimotope; Phage display; Pit viper; Polyclone antibody; Snake venom