title: Simultaneous, rapid, and sensitive quantification of 8-hydroxy-2'-deoxyguanosine and cotinine in human urine by on-line solid-phase extraction LC-MS/MS: correlation with tobacco exposure biomarkers NNAL.
abstract: Cigarette smoke can increase oxidative DNA damage. The main component in cigarette smoke is nicotine. Nicotine is metabolized to cotinine, which can be regarded as a biomarker for measuring exposure to tobacco smoke. A sensitive, simple, and robust method based on on-line solid-phase extraction liquid chromatography with tandem mass spectrometry (on-line SPE LC-MS/MS) has been developed and validated for the simultaneous determination of 8-OHdG and cotinine. The matrix effects of 8-OHdG and cotinine were measured at 97.1 and 91.7 %, with values for CV at 4.4 and 4.2 %, respectively. The limits of detection of 8-OHdG and cotinine were 10.0 and 5.5 pg mL(-1), and the limits of quantification were 40.0 and 20.0 pg mL(-1), respectively. The total run time was 12 min. We quantified 8-OHdG and cotinine in the urine of 80 male subjects. The results showed the levels of 8-OHdG and cotinine in smokers were significantly higher than that in non-smokers. Furthermore, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its glucuronide conjugate (defined as total NNAL) are the nitrosation metabolites of nicotine. In this study, urinary levels of 8-OHdG and cotinine were well correlated with urinary levels of total NNAL. This is also the first study to focus on the future risk of oxidative stress from exposure to cigarette smoke based on the relationship between 8-OHdG levels, cotinine levels, and total NNAL concentrations in the urine of humans. Graphical Abstract On-line SPE LC-MS/MS for the simultaneous determination of 8-OHdG and cotinine in human urine.
authors.affiliation: Department of Chemistry, Fu Jen Catholic University, No. 510 Zhongzheng Road, Xinzhuang District, New Taipei City, 24205, Taiwan.
authors.forename: Chao-Yu
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Fu Jen Catholic University, No. 510 Zhongzheng Road, Xinzhuang District, New Taipei City, 24205, Taiwan.
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Jhou
authors.affiliation: Department of Chemistry, Fu Jen Catholic University, No. 510 Zhongzheng Road, Xinzhuang District, New Taipei City, 24205, Taiwan. 076308@mail.fju.edu.tw.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Lee
authors.affiliation: Department of Public Health, Fu Jen Catholic University, No. 510 Zhongzheng Road, Xinzhuang District, New Taipei City, 24205, Taiwan.
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Lin
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D002853:Chromatography, Liquid / Q000191:economics / Q000379:methods; D003367:Cotinine / Q000652:urine*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D008297:Male; D008875:Middle Aged; D009538:Nicotine / Q000652:urine; D052616:Solid Phase Extraction / Q000191:economics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*; D000073869:Tobacco Smoking / Q000009:adverse effects / Q000652:urine*
chemical_list: D009538:Nicotine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D003367:Cotinine
keywords: 8-OHdG; Cotinine; NNAL; On-line SPE LC-MS/MS; Oxidative stress