title: Substantially Complete Removal of Three Major Allergenic Soybean Proteins (Gly m Bd 30K, Gly m Bd 28K, and the α-Subunit of Conglycinin) from Soy Protein by Using a Mutant Soybean, Tohoku 124.
abstract: A wild-type soybean contains three major allergenic proteins, Gly m Bd 30K, the α-subunit of conglycinin, and Gly m Bd 28K. A genetically mutated soybean (Tohoku 124), which was originally developed as a cultivar lacking the α- and α'-subunits of conglycinin, was also found to lack Gly m Bd 28K from immunoblot analysis using monoclonal antibodies specific to Gly m Bd 28K. This finding indicates the possibility to prepare soy milk and soy proteins containing none of the three major allergenic soybean proteins from this cultivar. By applying the previous removal procedure [Samoto et al., Biosci. Biotech. Biochem., 60, 1911-1913 (1996)] to Tohoku 124, the substantially complete removal of the three major allergenic proteins from the soy milk was attained. The removal rates of Gly m Bd 30K, α-subunit of conglycinin, and Gly m Bd 28K were 99.8, 100, and 100%, respectively.
authors.affiliation: a Central Research Institute , Fuji Oil Co. , 4-3 Kinunodai, Yawara-mura, Ibaraki 300-24 , Japan.
authors.forename: M
authors.initials: M
authors.lastname: Samoto
authors.affiliation: a Central Research Institute , Fuji Oil Co. , 4-3 Kinunodai, Yawara-mura, Ibaraki 300-24 , Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuda
authors.affiliation: a Central Research Institute , Fuji Oil Co. , 4-3 Kinunodai, Yawara-mura, Ibaraki 300-24 , Japan.|b Tohoku National Agricultural Experiment Station , Nishisenboku-machi, Akita 019-21 , Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: a Central Research Institute , Fuji Oil Co. , 4-3 Kinunodai, Yawara-mura, Ibaraki 300-24 , Japan.|b Tohoku National Agricultural Experiment Station , Nishisenboku-machi, Akita 019-21 , Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tabuchi
authors.affiliation: a Central Research Institute , Fuji Oil Co. , 4-3 Kinunodai, Yawara-mura, Ibaraki 300-24 , Japan.|c Department of Nutrition, School of Medicine , The University of Tokushima , Kuramoto-cho 3, Tokushima 770 , Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hiemori
authors.affiliation: a Central Research Institute , Fuji Oil Co. , 4-3 Kinunodai, Yawara-mura, Ibaraki 300-24 , Japan.|c Department of Nutrition, School of Medicine , The University of Tokushima , Kuramoto-cho 3, Tokushima 770 , Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tsuji
authors.affiliation: a Central Research Institute , Fuji Oil Co. , 4-3 Kinunodai, Yawara-mura, Ibaraki 300-24 , Japan.|c Department of Nutrition, School of Medicine , The University of Tokushima , Kuramoto-cho 3, Tokushima 770 , Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.affiliation: a Central Research Institute , Fuji Oil Co. , 4-3 Kinunodai, Yawara-mura, Ibaraki 300-24 , Japan.|d Protein Science Laboratory, National Food Research Institute , 2-1-2 Kannondai, Tsukuba, Ibaraki 305 , Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kawamura
keywords: allergy; soybean protein; soymilk