title: Rapid and sensitive LC-MS/MS method for the determination of auraptene in rat plasma and its application in a pharmacokinetic and bioavailability study in rats.
abstract: A simple, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of auraptene, a constituent isolated from Fructus aurantii with potential to combat Alzheimer's disease, in rat plasma. Rat plasma samples were pretreated by protein precipitation with methanol. The analytes were separated by a Waters Sun Fire C18 column (50 mm x 2 mm, 5 μm) and eluted with 1:1000 methanol and formic acid/water (v/v) mobile phase with a flow rate of 0.5 mL/min. Multiple reaction monitoring was used to monitor the transition of the deprotonated auraptene molecule with an m/z of 299.3 [M+H](+), to the product ion with an m/z of 162.9 [M+H](+). Progesterone, with an m/z of 315.2→ 96.9 was used as an internal standard. The limits of detection and of quantification of auraptene in the rat plasma were 1 and 5 ng/mL, respectively. The method was linear in the concentration range of 20- 2000 ng/mL with coefficient correlation of 0.9956. After auraptene (100 mg/kg, p.o.) administration, the maximum plasma concentration and the time taken to reach maximum concentration were 1719.5 ± 384.3 g/mL and 108.0 ± 25.3 min, respectively. The elimination half-life was 108.0 ± 25.3 for auraptene (100 mg/kg, p.o.) and 3.0 ± 0 min for auraptene (2 mg/kg, i.v.). The oral bioavailability was about 8.5%.
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
authors.forename: X D
authors.initials: XD
authors.lastname: Ye
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, China.|State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, China.
authors.forename: H
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Zhong
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
authors.forename: T E
authors.initials: TE
authors.lastname: Li
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Rao
authors.affiliation: State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Feng
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
authors.forename: W L
authors.initials: WL
authors.lastname: Yang
mesh_terms: D000818:Animals; D001682:Biological Availability; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards; D003374:Coumarins / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D013058:Mass Spectrometry / Q000379:methods* / Q000592:standards; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
chemical_list: D003374:Coumarins; C105832:aurapten