title: Large-Scale Metabolite Analysis of Standards and Human Serum by Laser Desorption Ionization Mass Spectrometry from Silicon Nanopost Arrays.
abstract: The unique challenges presented by metabolomics have driven the development of new mass spectrometry (MS)-based techniques for small molecule analysis. We have previously demonstrated silicon nanopost arrays (NAPA) to be an effective substrate for laser desorption ionization (LDI) of small molecules for MS. However, the utility of NAPA-LDI-MS for a wide range of metabolite classes has not been investigated. Here we apply NAPA-LDI-MS to the large-scale acquisition of high-resolution mass spectra and tandem mass spectra from a collection of metabolite standards covering a range of compound classes including amino acids, nucleotides, carbohydrates, xenobiotics, lipids, and other classes. In untargeted analysis of metabolite standard mixtures, detection was achieved for 374 compounds and useful MS/MS spectra were obtained for 287 compounds, without individual optimization of ionization or fragmentation conditions. Metabolite detection was evaluated in the context of 31 metabolic pathways, and NAPA-LDI-MS was found to provide detection for 63% of investigated pathway metabolites. Individual, targeted analysis of the 20 common amino acids provided detection of 100% of the investigated compounds, demonstrating that improved coverage is possible through optimization and targeting of individual analytes or analyte classes. In direct analysis of aqueous and organic extracts from human serum samples, spectral features were assigned to a total of 108 small metabolites and lipids. Glucose and amino acids were quantitated within their physiological concentration ranges. The broad coverage demonstrated by this large-scale screening experiment opens the door for use of NAPA-LDI-MS in numerous metabolite analysis applications.
authors.affiliation: Department of Chemistry, George Washington University , 800 22nd Street, North West, Washington, District of Columbia 20052, United States.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Korte
authors.affiliation: Department of Chemistry, George Washington University , 800 22nd Street, North West, Washington, District of Columbia 20052, United States.
authors.forename: Sylwia A
authors.initials: SA
authors.lastname: Stopka
authors.affiliation: Protea Biosciences, Inc. , Morgantown, West Virginia 26505, United States.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Morris
authors.affiliation: Protea Biosciences, Inc. , Morgantown, West Virginia 26505, United States.
authors.forename: Trust
authors.initials: T
authors.lastname: Razunguzwa
authors.affiliation: Department of Chemistry, George Washington University , 800 22nd Street, North West, Washington, District of Columbia 20052, United States.
authors.forename: Akos
authors.initials: A
authors.lastname: Vertes
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000097:blood / Q000378:metabolism; D004867:Equipment Design; D006801:Humans; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods*; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D044967:Serum / Q000737:chemistry / Q000378:metabolism; D012825:Silicon / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods
chemical_list: D000596:Amino Acids; D012825:Silicon