title: Gold nanoparticle-DNA aptamer conjugate-assisted delivery of antimicrobial peptide effectively eliminates intracellular Salmonella enterica serovar Typhimurium.
abstract: Antimicrobial peptides (AMPs) are a promising new class of antibacterial compounds. However, their applications in the treatment of intracellular pathogenic bacteria have been limited by their in vivo instability and low penetrating ability into mammalian cells. Here, we report that gold nanoparticles conjugated with DNA aptamer (AuNP-Apt) efficiently delivered AMPs into mammalian living systems with enhanced stability of the AMPs. C-terminally hexahistidine-tagged A3-APO (A3-APO(His)) AMPs were loaded onto AuNPs conjugated with His-tag DNA aptamer (AuNP-Apt(His)) by simple mixing and were delivered into Salmonella enterica serovar Typhimurium (S. Typhimurium)-infected HeLa cells, resulting in the increased viability of host cells due to the elimination of intracellular S. Typhimurium cells. Furthermore, the intravenous injection of AuNP-Apt(His) loaded with A3-APO(His) into S. Typhimurium-infected mice resulted in a complete inhibition of S. Typhimurium colonization in the mice organs, leading to 100% survival of the mice. Therefore, AuNP-Apt(His) can serve as an innovative platform for AMP therapeutics to treat intracellular bacterial infections in mammals.
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Yeom
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Boeun
authors.initials: B
authors.lastname: Lee
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Daeyoung
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, Chosun University, Gwangju 61452, Republic of Korea.
authors.forename: Jong-Kook
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea.
authors.forename: Suk
authors.initials: S
authors.lastname: Kim
authors.affiliation: School of Pharmacy, Chung-Ang University, Seoul 06974, Republic of Korea. Electronic address: jeehyeon@cau.ac.kr.
authors.forename: Jeehyeon
authors.initials: J
authors.lastname: Bae
authors.affiliation: Department of Biomedical Science, Chosun University, Gwangju 61452, Republic of Korea. Electronic address: y_k_park@chosun.ac.kr.
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Park
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea. Electronic address: kangseok@cau.ac.kr.
authors.forename: Kangseok
authors.initials: K
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D023181:Antimicrobial Cationic Peptides / Q000008:administration & dosage* / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D058726:Nanoconjugates / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D012485:Salmonella typhi / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D052157:Aptamers, Nucleotide; D053769:Nanocapsules; D058726:Nanoconjugates; D006046:Gold
keywords: A3-APO; Antimicrobial peptides; Gold-nanoparticle; S.Typhimurium