title: Unusual mode of protein binding by a cytotoxic π-arene ruthenium(ii) piano-stool compound containing an O,S-chelating ligand.
abstract: A new pseudo-octahedral π-arene ruthenium(ii) piano-stool compound, containing an O,S-bidentate ligand (compound 1) and showing significant cytotoxic activity in vitro, was synthesized and characterized. In solution stability and interaction with the model protein bovine pancreatic ribonuclease (RNase A) were investigated by using UV-Vis absorption spectroscopy. Its crystal structure and that of the adduct formed upon reaction with RNase A were obtained by X-ray crystallography. The comparison between the structure of purified compound 1 and that of the fragment bound to RNase A reveals an unusual mode of protein binding that includes ligand exchange and alteration of coordination sphere geometry.
authors.affiliation: Department of Inorganic and Analytical Chemistry, University of Jena, Germany. wolfgang.weigand@uni-jena.de.
authors.forename: Jana
authors.initials: J
authors.lastname: Hildebrandt
authors.affiliation: Department of Inorganic and Analytical Chemistry, University of Jena, Germany. wolfgang.weigand@uni-jena.de.
authors.forename: Helmar
authors.initials: H
authors.lastname: Görls
authors.affiliation: Department of Gynecology, Jena University Hospital - Friedrich-Schiller-University Jena, Germany.
authors.forename: Norman
authors.initials: N
authors.lastname: Häfner
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Italy. antonello.merlino@unina.it.
authors.forename: Giarita
authors.initials: G
authors.lastname: Ferraro
authors.affiliation: Department of Gynecology, Jena University Hospital - Friedrich-Schiller-University Jena, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Dürst
authors.affiliation: Department of Gynecology, Jena University Hospital - Friedrich-Schiller-University Jena, Germany.
authors.forename: Ingo B
authors.initials: IB
authors.lastname: Runnebaum
authors.affiliation: Department of Inorganic and Analytical Chemistry, University of Jena, Germany. wolfgang.weigand@uni-jena.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Weigand
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Italy. antonello.merlino@unina.it and Institute of Biostructures and Bioimages, Naples, Italy.
authors.forename: Antonello
authors.initials: A
authors.lastname: Merlino
mesh_terms: D002614:Chelating Agents / Q000737:chemistry*; D056831:Coordination Complexes / Q000737:chemistry*; D018360:Crystallography, X-Ray; D008024:Ligands; D009942:Organometallic Compounds / Q000737:chemistry*; D011485:Protein Binding; D012259:Ribonuclease, Pancreatic / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002614:Chelating Agents; D056831:Coordination Complexes; D008024:Ligands; D009942:Organometallic Compounds; D012428:Ruthenium; D012259:Ribonuclease, Pancreatic