title: Efficient production of nonactin by Streptomyces griseus subsp. griseus.
abstract: Here we report the production of the cyclic macrotetrolide nonactin from the fermentation culture of Streptomyces griseus subsp. griseus. Nonactin is a member of a family of naturally occurring cyclic ionophores known as the macrotetrolide antibiotics. Our fermentation procedure of Streptomyces griseus was performed at 30 °C and 200 rev·min(-1) for 5 days on a rotary shaker. Diaion HP-20 and Amberlite XAD-16 were added to the fermentation medium. Isolated yield of nonactin was up to 80 mg·L(-1) using our methodology. Nonactin is commonly known as an ammonium ionophore and also exhibits antibacterial, antiviral, and antitumor activities. It is also widely used for the preparation of ion-selective electrodes and sensors. Chemical synthesis of nonactin has been achieved by some groups; however, overall yields are very low, making efficient biosynthesis an attractive means of production.
authors.affiliation: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, People's Republic of China.|School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, People's Republic of China.
authors.forename: Yulian
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, People's Republic of China.|School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, People's Republic of China.
authors.forename: Shaolun
authors.initials: S
authors.lastname: Zheng
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism; D011108:Polymers; D013305:Streptomyces griseus / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; D011108:Polymers; C074337:Amberlite XAD-16; C050476:nonactin
keywords: Streptomyces; fermentation; macrotetrolides; macrotétrolides; nonactin; nonactine