title: Memory deficit, toxic effects and activity of Na(+), K(+)-ATPase and NTPDase in brain of Wistar rats submitted to orally treatment with alpha-terpinene.
abstract: The neurotoxic effects and activity of Na(+), K(+)-ATPase and NTPDase in Wistar rats after treatment with α-terpinene (daily oral administration of 0.5, 0.75 and 1.0mLkg(-1) for 10days) were examined. Results of the inhibitory avoidance task showed a memory deficit (p<0.05) in rats treated with all doses of α-terpinene. The evaluation of DNA damage in brain tissue revealed an increase (p<0.05) on frequency of damage and damage index in all concentrations. According to the cytotoxicity assay, doses of 0.5, 0.75 and 1.0mLkg(-1) increase the lactate dehydrogenase levels, and doses of 1.0mLkg(-1) also decrease (p<0.05) cell viability in brain cells. A decrease (p<0.05) on Na(+), K(+)-ATPase activity in brain tissue and on NTPDase activity in serum were observed in all concentrations of α-terpinene. These results suggest that the α-terpinene was cytotoxic and genotoxic to the brain cells by inducing loss of cell viability and DNA damage, as well as causing alterations in Na(+), K(+)-ATPase and NTPDase activity, what may contribute to the memory deficit of treated animals. Thus, α-terpinene cannot be consumed by the population at the doses studied.
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. Electronic address: aleksandro_ss@yahoo.com.br.
authors.forename: Matheus D
authors.initials: MD
authors.lastname: Baldissera
authors.affiliation: Department of Physiology and Pharmacology, UFSM, Santa Maria, RS, Brazil.
authors.forename: Carine F
authors.initials: CF
authors.lastname: Souza
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Thirssa H
authors.initials: TH
authors.lastname: Grando
authors.affiliation: Laboratory of Cell Culture, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Michele R
authors.initials: MR
authors.lastname: Sagrillo
authors.affiliation: Laboratory of Cell Culture, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Gerson F
authors.initials: GF
authors.lastname: De Brum
authors.affiliation: Laboratory of Cell Culture, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Kátia
authors.initials: K
authors.lastname: Nascimento
authors.affiliation: Laboratory of Cell Culture, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Diulle S
authors.initials: DS
authors.lastname: Peres
authors.affiliation: Laboratory of Cell Culture, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Miriãn F
authors.initials: MF
authors.lastname: Maciel
authors.affiliation: Department of Biochemistry, UFSM, Santa Maria, RS, Brazil.
authors.forename: Sérgio O
authors.initials: SO
authors.lastname: Silveira
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Sonia C A
authors.initials: SCA
authors.lastname: Da Luz
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Pedro H
authors.initials: PH
authors.lastname: Doleski
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Daniela B R
authors.initials: DBR
authors.lastname: Leal
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: da Silva
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Monteiro
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D000081005:Cyclohexane Monoterpenes; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D008569:Memory Disorders / Q000139:chemically induced*; D039821:Monoterpenes / Q000008:administration & dosage / Q000633:toxicity*; D009152:Mutagenicity Tests; D011755:Pyrophosphatases / Q000097:blood / Q000378:metabolism*; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; C018669:gamma-terpinene; D011755:Pyrophosphatases; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Alpha-terpinene; Cell viability; DNA damage; Genotoxicity; Serum