title: Lipolysis stimulating peptides of potato protein hydrolysate effectively suppresses high-fat-diet-induced hepatocyte apoptosis and fibrosis in aging rats.
abstract: BACKGROUND
  Non-alcoholic fatty liver disease (NAFLD) is one of the most common outcomes of obesity and is characterized by the accumulation of triglycerides, increased tissue apoptosis, and fibrosis. NAFLD is more common among elderly than in younger age groups, and it causes serious hepatic complications.
  
  
  OBJECTIVE
  In this study, alcalase treatment derived potato protein hydrolysate (APPH) with lipolysis-stimulating property has been evaluated for its efficiency to provide hepato-protection in a high-fat-diet (HFD)-fed aging rats.
  
  
  METHODS
  Twenty-four-month-old SD rats were randomly divided into six groups (n=8): aged rats fed with standard chow, HFD-induced aged obese rats, HFD with low-dose (15 mg/kg/day) APPH treatment, HFD with moderate (45 mg/kg/day) APPH treatment, HFD with high (75 mg/kg/day) APPH treatment, and HFD with probucol.
  
  
  RESULTS
  APPH was found to reduce the NAFLD-related effects in rat livers induced by HFD and all of the HFD-fed rats exhibited heavier body weight than those with control chow diet. However, the HFD-induced hepatic fat accumulation was effectively attenuated in rats administered with low (15 mg/kg/day), moderate (45 mg/kg/day), and high (75 mg/kg/day) doses of APPH. APPH oral administration also suppressed the hepatic apoptosis- and fibrosis-related proteins induced by HFD.
  
  
  CONCLUSIONS
  Our results thus indicate that APPH potentially attenuates hepatic lipid accumulation and anti-apoptosis and fibrosis effects in HFD-induced rats. APPH may have therapeutic potential in the amelioration of NAFLD liver damage.
authors.affiliation: Department of Food Science, College of Agriculture, Tunghai University, Taichung, Taiwan.
authors.forename: Wen-Dee
authors.initials: WD
authors.lastname: Chiang
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.|School of Chinese Medicine, China Medical University, Taichung, Taiwan.|Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
authors.forename: Chih Yang
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.|Department of Hospitality Management, College of Agriculture, Tunghai University, Taichung, Taiwan.
authors.forename: Catherine Reena
authors.initials: CR
authors.lastname: Paul
authors.affiliation: Department of Hospitality Management, College of Agriculture, Tunghai University, Taichung, Taiwan.
authors.forename: Zong-Yan
authors.initials: ZY
authors.lastname: Lee
authors.affiliation: Department of Hospitality Management, College of Agriculture, Tunghai University, Taichung, Taiwan; 040770@thu.edu.tw.
authors.forename: Wan-Teng
authors.initials: WT
authors.lastname: Lin
keywords: APPH; apoptosis; fibrosis; high fat diet; lipolysis stimulating protein hydrolysates; obesity