title: The cardioprotective effect of naringenin against ischemia-reperfusion injury through activation of ATP-sensitive potassium channel in rat.
abstract: Naringenin (Nari) has antioxidative and anti-atherosclerosis effects, and activation of ATP-sensitive potassium channel (KATP) can offer cardiac protection. We hypothesized that Nari protects the heart against ischemia-reperfusion (I-R) injury through activation of KATP. Isolated hearts from adult male Sprague-Dawley rats experienced a 30-min global ischemia followed by 60-min reperfusion (120 min for the infarct size determination). The hearts were treated with Nari (NARI); Nari plus glibenclamide (GLI), a non-specific ATP-sensitive potassium channel blocker (NARI+GLI); and Nari plus 5-hydroxy decanoic acid (5-HD), a mitochondrial membrane ATP-sensitive potassium channel blocker (NARI+5-HD). The left ventricular pressure, lactate dehydrogenates (LDH) in coronary effluent, superoxide dismutase (SOD) and malondialdehyde (MDA) in myocardium, and myocardial infarct area were measured. Nari above 2.5 μmol/L improved the recovery of left ventricular function, decreased LDH in coronary effluent, and reduced myocardial infarct area. The SOD activity was increased and MDA was decreased in Nari-treated myocardium. The cardioprotective effect of Nari was canceled by GLI and 5-HD. In conclusion, Nari has a cardioprotective effect against I-R injury, which may be carried out through activating ATP-sensitive potassium channels in both cell and mitochondrial membrane, and enhancing myocardial antioxidant capacity.
authors.affiliation: a Department of Physiology, Hebei Medical University, Shijiazhuang 050017, PR China.|b Second Department of Cardiology, General Hospital of FengFeng Branch in Center Hebei Energy Sources Group, Handan 056200, PR China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Meng
authors.affiliation: a Department of Physiology, Hebei Medical University, Shijiazhuang 050017, PR China.|d Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang, Hebei 050000, PR China.
authors.forename: Hui-Jie
authors.initials: HJ
authors.lastname: Ma
authors.affiliation: c Department of Gynaecology and Obstetrics, Fourth Hospital of Hebei Medical University, Shijiazhuang 050000, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.affiliation: a Department of Physiology, Hebei Medical University, Shijiazhuang 050017, PR China.|e Shandong Zaozhuang Vocational College of Technology, Tengzhou, Shandong 277500, PR China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Kong
authors.affiliation: a Department of Physiology, Hebei Medical University, Shijiazhuang 050017, PR China.|d Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang, Hebei 050000, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003652:Decanoic Acids / Q000494:pharmacology; D044950:Flavanones / Q000037:antagonists & inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D005905:Glyburide / Q000494:pharmacology; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D006880:Hydroxy Acids / Q000494:pharmacology; D054086:KATP Channels / Q000819:agonists* / Q000037:antagonists & inhibitors; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism* / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D026902:Potassium Channel Blockers / Q000494:pharmacology; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D002316:Cardiotonic Agents; D003652:Decanoic Acids; D044950:Flavanones; D006880:Hydroxy Acids; D054086:KATP Channels; D026902:Potassium Channel Blockers; D008315:Malondialdehyde; C052853:5-hydroxydecanoic acid; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; C005273:naringenin; D005905:Glyburide
keywords: ATP-sensitive potassium channels; antioxidation; antioxydation; canaux potassiques ATP-dépendants; cardiac protection; ischemia–reperfusion; ischémie–reperfusion; naringenin; naringénine; protection cardiaque