title: Characterization of aerobic oil and grease-degrading bacteria in wastewater.
abstract: A bacterial consortium that degrades cooking oil (CO) has been isolated in wastewater (WW) samples, by enrichment in olive CO. This consortium could degrade 90% of CO within 7-9 days (from an initial 1% [w/v]), and it is more active at alkaline conditions. The 16S ribonucleic acid (RNA) gene analysis showed that it contains five bacterium species: Stenotrophomonas rhizophila, Sphingobacterium sp., Pseudomonas libanensis, Pseudomonas poae and Pseudomonas aeruginosa. This consortium can degrade the free fatty acids (FFA): palmitic, stearic, oleic, linoleic and linolenic acids; glycerol, glucose and amylose; and albumin, but could not efficiently degrade carboxymethyl-cellulose. Each strain could also degrade CO and FFAs. The level of bacterial crude-activity of extracellular lipases was found to be between 0.2 and 4U/ml. Using synthetic WW, the consortium could reduce 80% of the chemical oxygen demand [from 10550 ± 2828 mg/l], 80% of nitrogen (from 410 ± 78 mgl/l) and 57% of phosphorus (from 93 ± 25 mg/l). Thus, this consortium can be utilized in the removal of CO from WW.
authors.affiliation: a Departments of Life Sciences , King Fahd University of Petroleum and Minerals , Dhahran , Saudi Arabia.
authors.forename: Alexis
authors.initials: A
authors.lastname: Nzila
authors.affiliation: a Departments of Life Sciences , King Fahd University of Petroleum and Minerals , Dhahran , Saudi Arabia.
authors.forename: Assad
authors.initials: A
authors.lastname: Thukair
authors.affiliation: a Departments of Life Sciences , King Fahd University of Petroleum and Minerals , Dhahran , Saudi Arabia.
authors.forename: Saravanan
authors.initials: S
authors.lastname: Sankara
authors.affiliation: b Department of Chemical Engineering, King Fahd University of Petroleum and Minerals , Dhahran , Saudi Arabia.
authors.forename: Shaikh
authors.initials: S
authors.lastname: Abdur Razzak
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D006838:Hydrocarbons / Q000378:metabolism*; D059013:Microbial Consortia; D010578:Petroleum / Q000378:metabolism* / Q000382:microbiology; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D062065:Wastewater / Q000382:microbiology*
chemical_list: D006838:Hydrocarbons; D010578:Petroleum; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D062065:Wastewater
keywords: Cooking oil; bacteria; biodegradation; chemical oxygen demand; wastewaters