title: Establishment and Analysis of Cancer Stem-Like and Non-Cancer Stem-Like Clone Cells from the Human Colon Cancer Cell Line SW480.
abstract: Human cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) can be isolated as side population (SP) cells, aldehyde dehydrogenase high (ALDHhigh) cells or cell surface marker-positive cells including CD44+ cells and CD133+ cells. CSCs/CICs and non-CSCs/CICs are unstable in in vitro culture, and CSCs/CICs can differentiate into non-CSCs/CICs and some non-CSCs/CICs can dedifferentiate into CSCs/CICs. Therefore, experiments using a large amount of CSCs/CICs are technically very difficult. In this study, we isolated single cell clones from SP cells and main population (MP) cells derived from the human colon cancer cell line SW480. SP analysis revealed that SP clone cells had relatively high percentages of SP cells, whereas MP clone cells showed very few SP cells, and the phenotypes were sustainable for more than 2 months of in vitro culture. Xenograft transplantation revealed that SP clone cells have higher tumor-initiating ability than that of MP clone cells and SP clone cell showed higher chemo-resistance compared with MP clone cells. These results indicate that SP clone cells derived from SW480 cells are enriched with CSCs/CICs, whereas MP clone cells are pure non-CSCs/CICs. SP clone cells and MP clone cells are a very stable in vitro CSC/CIC-enriched and non-CSC/CIC model for further analysis.
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.
authors.forename: Akari
authors.initials: A
authors.lastname: Takaya
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Hirohashi
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Murai
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.
authors.forename: Rena
authors.initials: R
authors.lastname: Morita
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.|Department of Respiratory Medicine and Allergology, Sapporo Medical University School of Medicine, Sapporo, 060-8543, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Saijo
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Yamamoto
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.
authors.forename: Terufumi
authors.initials: T
authors.lastname: Kubo
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.
authors.forename: Munehide
authors.initials: M
authors.lastname: Nakatsugawa
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kanaseki
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.
authors.forename: Tomohide
authors.initials: T
authors.lastname: Tsukahara
authors.affiliation: Department of Molecular Therapeutics, Center for Food & Medical Innovation, Hokkaido University, Sapporo, 060-8638, Japan.
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Tamura
authors.affiliation: Department of Surgery, Sapporo Medical University School of Medicine, Sapporo, 060-8543, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takemasa
authors.affiliation: Division of Stem Cell Biology, Institute for Genetic Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Kondo
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Torigoe
mesh_terms: D000818:Animals; D002453:Cell Cycle; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000502:physiology* / Q000637:transplantation; D058985:Side-Population Cells / Q000502:physiology* / Q000637:transplantation