title: Effect of Fluoride on Endocytosis and Surface Marker Expression Levels of Mouse B Cells In Vitro.
abstract: OBJECTIVE
  To clarify the effect of fluoride on splenic B cells, the endocytosis and surface marker expression levels of mouse splenic B cells were detected in vitro by flow cytometry.
  
  
  METHODS
  Cells were stimulated with 10 µg/mL lipopolysaccharide (LPS) and varying concentrations of Sodium Fluoride (NaF) (0, 50 µM, 100 µM, 500 µM, 1000 µM).
  
  
  RESULTS
  The results demonstrated that the endocytic capacity of B cells was enhanced by NaF at 50µM. NaF significantly enhanced CD80 expression at 50 µM and decreased CD86 expression at 500 µM. CD40 and CD138 expression on B cells were down-regulated at varying high concentrations of NaF.
  
  
  CONCLUSIONS
  our results showed that the endocytic capacity, expression levels of CD40 and CD80 of B cells changed significantly at lower concentrations, whereas expression levels of CD138 and CD86 changed significantly at higher concentrations, suggesting that fluoride could inhibit immune function in animals.
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, Taigu, PR China.
authors.forename: Haili
authors.initials: H
authors.lastname: Ma
authors.forename: Zeyu
authors.initials: Z
authors.lastname: Shi
authors.forename: Yaoze
authors.initials: Y
authors.lastname: Dong
authors.forename: Rui
authors.initials: R
authors.lastname: Liang
authors.forename: Jianshan
authors.initials: J
authors.lastname: Chen
authors.forename: Jinming
authors.initials: J
authors.lastname: Wang
authors.forename: Jundong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004705:Endocytosis / Q000187:drug effects*; D005260:Female; D005434:Flow Cytometry; D005459:Fluorides / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D012969:Sodium Fluoride / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D053668:Syndecan-1 / Q000378:metabolism
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D015415:Biomarkers; D019013:CD40 Antigens; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D053668:Syndecan-1; D012969:Sodium Fluoride; D005459:Fluorides