title: Proteomic analysis of JAZ interacting proteins under methyl jasmonate treatment in finger millet.
abstract: Jasmonic acid (JA) signaling pathway in plants is activated against various developmental processes as well as biotic and abiotic stresses. The Jasmonate ZIM-domain (JAZ) protein family, the key regulator of plant JA signaling pathway, also participates in phytohormone crosstalk. This is the first study revealing the in vivo interactions of finger millet (Eleusine coracana (L.) Gaertn.) JAZ protein (EcJAZ) under methyl jasmonate (MJ) treatment. The aim of the study was to explore not only the JA signaling pathway but also the phytohormone signaling crosstalk of finger millet, a highly important future crop. From the MJ-treated finger millet seedlings, the EcJAZ interacting proteins were purified by affinity chromatography with the EcJAZ-matrix. Twenty-one proteins of varying functionalities were successfully identified by MALDI-TOF-TOF Mass spectrometry. Apart from the previously identified JAZ binding proteins, most prominently, EcJAZ was found to interact with transcription factors like NAC, GATA and also with Cold responsive protein (COR), etc. that might have extended the range of functionalities of JAZ proteins. Moreover, to evaluate the interactions of EcJAZ in the JA-co-receptor complex, we generated ten in-silico models containing the EcJAZ degron and the COI1-SKP1 of five monocot cereals viz., rice, wheat, maize, Sorghum and Setaria with JA-Ile or coronatine. Our results indicated that the EcJAZ protein of finger millet could act as the signaling hub for the JA and other phytohormone signaling pathways, in response to a diverse set of stressors and developmental cues to provide survival fitness to the plant.
authors.affiliation: Drug Development/Diagnostics and Biotechnology Division, CSIR - Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Kolkata, 700 032, India. Electronic address: sensaswati04@yahoo.com.
authors.forename: Saswati
authors.initials: S
authors.lastname: Sen
authors.affiliation: Structural Biology and Bioinformatics Division, CSIR - Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Kolkata, 700 032, India.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Kundu
authors.affiliation: Drug Development/Diagnostics and Biotechnology Division, CSIR - Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Kolkata, 700 032, India.
authors.forename: Samir Kr
authors.initials: SK
authors.lastname: Dutta
mesh_terms: D000085:Acetates / Q000378:metabolism / Q000494:pharmacology*; D000596:Amino Acids / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism; D003198:Computer Simulation; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology*; D031748:Eleusine / Q000187:drug effects / Q000378:metabolism*; D007192:Indenes / Q000378:metabolism; D008958:Models, Molecular; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology*; D010710:Phosphates / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction; D040681:Structural Homology, Protein
chemical_list: D000085:Acetates; D000596:Amino Acids; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D007192:Indenes; C554741:JAZ1 protein, Arabidopsis; D054883:Oxylipins; D010710:Phosphates; D010940:Plant Proteins; D011994:Recombinant Proteins; D012097:Repressor Proteins; C058487:coronatine; C072239:methyl jasmonate
keywords: Finger millet; In-silico modeling; JA co-receptor complex; JAZ; JAZ-interacting proteins; Jasmonate ZIM domain protein; Proteomics analysis