title: Expression of Pentraxin 3 and Thrombospondin 1 in Gingival Crevicular Fluid during Wound Healing after Gingivectomy in Postorthodontic Patients.
abstract: Background. Wound healing is a tissue repair process after an injury, and two of its main components are inflammation and angiogenesis, in which course a cascade of mediators is involved. The aim of this research was to evaluate the involvement of Pentraxin 3 and Thrombospondin 1 in wound healing after periodontal surgery (gingivectomy) for gingival overgrowth during orthodontic treatment with or without magnification devices, by assessing their levels in GCF. Methods. From 19 patients with gingival overgrowth as a result of fixed orthodontic treatment, the overgrown gingiva was removed by gingivectomy, from one half of the mandibular arch without magnification and from the other under magnification. Pentraxin 3 and Thrombospondin 1 were determined from gingival crevicular fluid by ELISA tests. Results. Statistically significant differences (p < 0.05) and correlations between levels of the two biomarkers were analyzed. Statistically significant differences were established between levels of the two biomarkers at different time points, with significant positive correlation at the point of 24 hours. Conclusions. Within the limitations of this study, the results seem to sustain the involvement of Pentraxin 3 and Thrombospondin 1 in the processes of inflammation and angiogenesis in wound healing of patients with postorthodontic gingivectomy. The dynamics of Pentraxin 3 and Thrombospondin 1 levels could suggest a reduced inflammation and a faster angiogenesis using microsurgery.
authors.affiliation: Department of Orthodontics, University of Medicine and Pharmacy, Strada Petru Rares 2, 200349 Craiova, Romania.
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Rauten
authors.affiliation: Department of Immunology, University of Medicine and Pharmacy, Strada Petru Rares 2, 200349 Craiova, Romania.
authors.forename: Isabela
authors.initials: I
authors.lastname: Silosi
authors.affiliation: Department of Periodontology, Victor Babes University of Medicine and Pharmacy, P-ta Eftimie Murgu 2A, 300041 Timisoara, Romania.
authors.forename: Stefan Ioan
authors.initials: SI
authors.lastname: Stratul
authors.affiliation: Department of Biochemistry, Grigore T. Popa University of Medicine and Pharmacy, Strada Universitatii 16, 700115 Iasi, Romania.
authors.forename: Liliana
authors.identifier: 0000-0002-7558-0711
authors.initials: L
authors.lastname: Foia
authors.affiliation: Department of Oral Surgery, University of Medicine and Pharmacy, Strada Petru Rares 2, 200349 Craiova, Romania.
authors.forename: Adrian
authors.identifier: 0000-0002-4680-8975
authors.initials: A
authors.lastname: Camen
authors.affiliation: Department of Surgery, Grigore T. Popa University of Medicine and Pharmacy, Strada Universitatii 16, 700115 Iasi, Romania.
authors.forename: Vasilica
authors.initials: V
authors.lastname: Toma
authors.affiliation: Department of Surgery, Grigore T. Popa University of Medicine and Pharmacy, Strada Universitatii 16, 700115 Iasi, Romania.
authors.forename: Daniel
authors.identifier: 0000-0002-7136-7825
authors.initials: D
authors.lastname: Cioloca
authors.affiliation: Department of Surgery, University of Medicine and Pharmacy, Strada Petru Rares 2, 200349 Craiova, Romania.
authors.forename: Valeriu
authors.identifier: 0000-0003-2648-5408
authors.initials: V
authors.lastname: Surlin
authors.affiliation: Department of Periodontology, University of Medicine and Pharmacy, Strada Petru Rares 2, 200349 Craiova, Romania.
authors.forename: Petra
authors.identifier: 0000-0003-3469-8008
authors.initials: P
authors.lastname: Surlin
authors.affiliation: Department of Pharmacology, University of Medicine and Pharmacy, Strada Petru Rares 2, 200349 Craiova, Romania.
authors.forename: Maria
authors.identifier: 0000-0001-8089-1787
authors.initials: M
authors.lastname: Bogdan
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005881:Gingiva / Q000503:physiopathology*; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D005890:Gingivectomy; D006801:Humans; D007249:Inflammation; D008297:Male; D018919:Neovascularization, Physiologic; D009970:Orthodontics; D000683:Serum Amyloid P-Component / Q000235:genetics / Q000378:metabolism*; D000072836:Surgical Wound / Q000503:physiopathology*; D019700:Thrombospondin 1 / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing; D055815:Young Adult; D000099153:Pentraxins
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein; D000683:Serum Amyloid P-Component; D019700:Thrombospondin 1; D000099153:Pentraxins