title: Development of Alginate Microspheres Containing Chuanxiong for Oral Administration to Adult Zebrafish.
abstract: Oral administration of Traditional Chinese Medicine (TCM) by patients is the common way to treat health problems. Zebrafish emerges as an excellent animal model for the pharmacology investigation. However, the oral delivery system of TCM in zebrafish has not been established so far. This issue was addressed by development of alginate microparticles for oral delivery of chuanxiong, a TCM that displays antifibrotic and antiproliferative effects on hepatocytes. The delivery microparticles were prepared from gelification of alginate containing various levels of chuanxiong. The chuanxiong-encapsulated alginate microparticles were characterized for their solubility, structure, encapsulation efficiency, the cargo release profile, and digestion in gastrointestinal tract of zebrafish. Encapsulation of chuanxiong resulted in more compact structure and the smaller size of microparticles. The release rate of chuanxiong increased for alginate microparticles carrying more chuanxiong in simulated intestinal fluid. This remarkable feature ensures the controlled release of encapsulated cargos in the gastrointestinal tract of zebrafish. Moreover, chuanxiong-loaded alginate microparticles were moved to the end of gastrointestinal tract after oral administration for 6 hr and excreted from the body after 16 hr. Therefore, our developed method for oral administration of TCM in zebrafish is useful for easy and rapid evaluation of the drug effect on disease.
authors.affiliation: School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan.
authors.forename: Li-Jen
authors.initials: LJ
authors.lastname: Lin
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 40402, Taiwan.
authors.forename: Chung-Jen
authors.initials: CJ
authors.lastname: Chiang
authors.affiliation: Department of Chemical Engineering, Feng Chia University, Taichung 40724, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan.
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Chao
authors.affiliation: School of Post-Baccalaureate Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan.
authors.forename: Shulhn-Der
authors.initials: SD
authors.lastname: Wang
authors.affiliation: Department of Chemical Engineering, Feng Chia University, Taichung 40724, Taiwan.
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Chiou
authors.affiliation: Department of Chemical Engineering, Feng Chia University, Taichung 40724, Taiwan.
authors.forename: Han-Yu
authors.initials: HY
authors.lastname: Wang
authors.affiliation: School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan; Department of Chinese Medicine, China Medical University Hospital, Taichung 40402, Taiwan.
authors.forename: Shung-Te
authors.initials: ST
authors.lastname: Kao
mesh_terms: D000284:Administration, Oral; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002214:Capsules / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000138:chemical synthesis / Q000493:pharmacokinetics*; D004058:Diffusion; D004339:Drug Compounding / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D041981:Gastrointestinal Tract / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D014018:Tissue Distribution; D015027:Zebrafish
chemical_list: D000464:Alginates; D002214:Capsules; D003692:Delayed-Action Preparations; D004365:Drugs, Chinese Herbal; D006603:Hexuronic Acids; C534193:chuanxiong rhizome preparation; D020723:Glucuronic Acid