title: Preferable existence of polyunsaturated lysophosphatidic acids in human follicular fluid from patients programmed with in vitro fertilization.
abstract: Lysophosphatidic acid (LPA) exerts diverse physiological effects on various types of animal cells, including reproductive cells, through its binding to six LPA receptors. We previously found that LPA promoted maturation of the nucleus and cytoplasm of mouse and hamster oocytes surrounded by cumulus cells in vitro. Using gas-liquid chromatography, we previously reported detection of several species of LPA by analyzing the fatty acid methyl esters derived from thin layer chromatography-purified LPA in lipid extract from incubated follicular fluids programmed with in vitro fertilization. In this study using liquid chromatography- tandem mass spectrometry, we directly detected high levels of linoleoyl, arachidonoyl, and docosahexaenoyl LPAs in human follicular fluid. This unique molecular species composition of LPA was suggested to be due to a balance between the low LPA-degrading activity and high LPA-producing activity of autotaxin in human follicular fluid. Our results suggest that polyunsaturated LPAs produced by autotaxin in human follicular fluid exert unknown physiological effects on cumulus cells.
authors.affiliation: Department of Pharmaceutical Health Chemistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Shomachi, Tokushima 770-8505, Japan.
authors.forename: Junpei
authors.initials: J
authors.lastname: Yamamoto
authors.affiliation: Department of Pharmaceutical Health Chemistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Shomachi, Tokushima 770-8505, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Omura
authors.affiliation: Department of Medical Pharmacology, Institute of Biomedical Sciences, Tokushima University Graduate School, Shomachi, Tokushima 770-8505, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Tuchiya
authors.affiliation: Faculty of Pharmacy, Yasuda Women's University, Yasuhigashi, Asaminami-ku, Hiroshima 731-0153, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Hidaka
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Biomedical Sciences, Tokushima University Graduate School, Kuramotocho, Tokushima 770-8503, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kuwahara
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Biomedical Sciences, Tokushima University Graduate School, Kuramotocho, Tokushima 770-8503, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Irahara
authors.affiliation: Department of Pharmaceutical Health Chemistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Shomachi, Tokushima 770-8505, Japan.
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Pharmaceutical Health Chemistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Shomachi, Tokushima 770-8505, Japan; Faculty of Pharmacy, Yasuda Women's University, Yasuhigashi, Asaminami-ku, Hiroshima 731-0153, Japan. Electronic address: tokumura@yasuda-u.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Tokumura
mesh_terms: D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D008246:Lysophospholipids / Q000737:chemistry* / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D000099189:Lysophospholipase D
chemical_list: D005231:Fatty Acids, Unsaturated; D008246:Lysophospholipids; D010727:Phosphoric Diester Hydrolases; D000099189:Lysophospholipase D
keywords: Liquid chromatography-tandem mass spectrometry; Lysophosphatidylcholine; Lysophospholipase D; Ovary