title: Effect of Vesicle-to-Micelle Transition on the Interactions of Phospholipid/Sodium Cholate Mixed Systems with Curcumin in Aqueous Solution.
abstract: The role of vesicle-to-micelle transition has been investigated in the interactions of phospholipid vesicles, phospholipid/sodium cholate (NaC) mixed vesicles, and phospholipid/NaC mixed micelles with curcumin in aqueous solution. The addition of NaC causes phospholipid vesicles to transit into phospholipid/NaC mixed vesicles and phospholipid/NaC mixed micelles. Turbidity measurement reveals that the presence of curcumin increases the NaC concentration for the solubilization of phospholipid vesicles, which indicates that the bound curcumin tends to suppress the vesicle-to-micelle transition. The pyrene polarity index and curcumin fluorescence anisotropy measurements suggest that phospholipid/NaC mixed micelles have a more compact structure than that of phospholipid vesicles and phospholipid/NaC mixed vesicles. Curcumin associated with phospholipid vesicles, phospholipid/NaC mixed vesicles, and phospholipid/NaC mixed micelles often results in higher intensities of absorption and fluorescence than those of free curcumin. However, phospholipid/NaC mixed vesicles lead to the highest values of absorption and fluorescence intensities, binding constant, and radical-scavenging capacity with curcumin. The different structures in the phospholipid bilayer of phospholipid/NaC mixed vesicles and the hydrophobic part of phospholipid/NaC mixed micelles where curcumin located are discussed to explain the interaction behaviors of phospholipid/NaC mixed systems with curcumin.
authors.affiliation: School of Chemistry and Molecular Engineering, East China University of Science and Technology , Shanghai 200237, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Molecular Engineering, East China University of Science and Technology , Shanghai 200237, China.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Wang
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D003474:Curcumin / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D005454:Fluorescence Polarization; D016166:Free Radical Scavengers / Q000737:chemistry; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D010743:Phospholipids / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D020358:Sodium Cholate / Q000737:chemistry*; D013053:Spectrophotometry; D014867:Water / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D004337:Drug Carriers; D016166:Free Radical Scavengers; D008823:Micelles; D010743:Phospholipids; D010851:Picrates; D014867:Water; C004931:1,1-diphenyl-2-picrylhydrazyl; D003474:Curcumin; D020358:Sodium Cholate