title: Hepatic expression of FTO and fatty acid metabolic genes changes in response to lipopolysaccharide with alterations in m6A modification of relevant mRNAs in the chicken.
abstract: The fat mass and obesity associated (FTO) gene, which encodes a demethylase of m6A, has been reported to respond to lipopolysaccharide (LPS) and to serve as a link between inflammation and metabolic responses. The objective of this study was to determine whether LPS-induced changes in the expression of FTO and metabolic genes are associated with alterations of m6A in relevant mRNAs. LPS challenge significantly decreased hepatic mRNA expression of carnitine palmitoyl transferase 1 (CPT1) and CPT2, which coincided with a tendency of higher triglyceride accumulation in the liver. LPS significantly down-regulated the full length cFTO1, yet up-regulated the truncated cFTO4 protein in the liver nuclear extracts. Nuclear protein content of cFTO4 in the liver was negatively correlated with the mRNA abundances of CPT1 (r = 0.629) and CPT2 (r = 0.622). Methylated RNA immunoprecipitation analysis revealed that the m6A level around the translation start site of CPT1 was markably decreased in the liver of LPS-treated chickens. These results indicate that LPS-induced changes in FTO protein expression are associated with alteration of mRNA m6A modification in chicken liver.
authors.affiliation: a Key Laboratory of Animal Physiology & Biochemistry , Nanjing Agricultural University , Nanjing , China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a Key Laboratory of Animal Physiology & Biochemistry , Nanjing Agricultural University , Nanjing , China.
authors.forename: F
authors.initials: F
authors.lastname: Guo
authors.affiliation: a Key Laboratory of Animal Physiology & Biochemistry , Nanjing Agricultural University , Nanjing , China.|b Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control , Nanjing , China.
authors.forename: R
authors.initials: R
authors.lastname: Zhao
mesh_terms: D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D002645:Chickens / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D008070:Lipopolysaccharides; D020033:Protein Isoforms; D012333:RNA, Messenger; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO