title: Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 1. High abundance proteins, acute phase proteins and peptidomics.
abstract: A peptidomic investigation of milk from an experimental model of Streptococcus uberis mastitis in dairy cows has incorporated a study of milk high abundance and acute phase (APP) proteins as well as analysis of low molecular weight peptide biomarkers. Intramammary infection (IMI) with S. uberis caused a shift in abundance from caseins, β-lactoglobulin and α-lactalbumin to albumin, lactoferrin and IgG with the increase in lactoferrin occurring last. The APP response of haptoglobin, mammary associated serum amyloid A3 and C-reactive protein occurred between 30-48 hours post challenge with peak concentrations of APPs at 72-96 hours post challenge and declined thereafter at a rate resembling the fall in bacterial count rather than the somatic cell count. A peptide biomarker panel for IMI based on capillary electrophoresis and mass spectrometry was developed. It comprised 77 identified peptides (IMI77) composed mainly of casein derived peptides but also including peptides of glycosylation dependent cell adhesion molecule and serum amyloid A. The panel had a biomarker classification score that increased from 36 hour to 81 hour post challenge, significantly differentiating infected from non-infected milk, thus suggesting potential as a peptide biomarker panel of bovine mastitis and specifically that of S. uberis origin. The use of omic technology has shown a multifactorial cross system reaction in high and low abundance proteins and their peptide derivatives with changes of over a thousand fold in analyte levels in response to S. uberis infection.
authors.affiliation: Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow, Bearsden Road, Glasgow, G61 1QH, UK. david.eckersall@glasgow.ac.uk.
authors.forename: Funmilola Clara
authors.initials: FC
authors.lastname: Thomas
authors.affiliation: Institute of Cardiovascular and Medical Sciences, University of Glasgow, University Avenue, Glasgow, UK.
authors.forename: William
authors.initials: W
authors.lastname: Mullen
authors.affiliation: Moredun Research Institute, Pentlands Science Park/Bush Loan, Penicuik, UK.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Tassi
authors.affiliation: Mosaique Diagnostics GmbH, Rotenburger Str. 20, D-30659 Hannover, Germany.
authors.forename: Adela
authors.initials: A
authors.lastname: Ramírez-Torres
authors.affiliation: Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow, Bearsden Road, Glasgow, G61 1QH, UK. david.eckersall@glasgow.ac.uk and Glasgow Polyomics, College of Medical, Veterinary and Life Science, University of Glasgow, Glasgow, UK.
authors.forename: Manikhandan
authors.initials: M
authors.lastname: Mudaliar
authors.affiliation: Moredun Research Institute, Pentlands Science Park/Bush Loan, Penicuik, UK.
authors.forename: Tom N
authors.initials: TN
authors.lastname: McNeilly
authors.affiliation: Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow, Bearsden Road, Glasgow, G61 1QH, UK. david.eckersall@glasgow.ac.uk and Moredun Research Institute, Pentlands Science Park/Bush Loan, Penicuik, UK.
authors.forename: Ruth N
authors.initials: RN
authors.lastname: Zadoks
authors.affiliation: Glasgow Polyomics, College of Medical, Veterinary and Life Science, University of Glasgow, Glasgow, UK.
authors.forename: Richard
authors.initials: R
authors.lastname: Burchmore
authors.affiliation: Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow, Bearsden Road, Glasgow, G61 1QH, UK. david.eckersall@glasgow.ac.uk.
authors.forename: P
authors.initials: P
authors.lastname: David Eckersall
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers; D002417:Cattle; D002853:Chromatography, Liquid; D005260:Female; D013058:Mass Spectrometry; D008414:Mastitis, Bovine / Q000378:metabolism* / Q000382:microbiology; D008892:Milk / Q000378:metabolism*; D008894:Milk Proteins / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D013290:Streptococcal Infections / Q000662:veterinary*; D013291:Streptococcus
chemical_list: D000209:Acute-Phase Proteins; D015415:Biomarkers; D008894:Milk Proteins; D010455:Peptides