title: Bioluminogenic Imaging of AminopeptidaseN In Vitro and In Vivo.
abstract: Bioluminescence is a process that converts biochemical energy to visible light. Bioluminescence-based imaging technology has been widely applicable in the imaging of process envisioned in life sciences. As one of the most popular bioluminescence system, the firefly luciferin-luciferase system is exceptionally suitable for deep tissue imaging in living animals owing to its long wavelength emission light. Herein, we report the experimental detail of bioluminogenic imaging of aminopeptidase N activity both in vitro and in vivo.
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Wenxiao
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Laizhong
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Lupei
authors.initials: L
authors.lastname: Du
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, 250012, China. mli@sdu.edu.cn.
authors.forename: Minyong
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D018826:CD13 Antigens / Q000378:metabolism*; D002460:Cell Line; D066298:In Vitro Techniques; D049409:Luciferases, Firefly / Q000378:metabolism; D049408:Luminescent Agents; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*
chemical_list: D049408:Luminescent Agents; D049409:Luciferases, Firefly; D018826:CD13 Antigens
keywords: Aminopeptidase N; Bioluminescence; Caged luciferin; Living animal imaging