title: OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2signalling in rice.
abstract: Drought is one of the major abiotic stresses that directly implicate plant growth and crop productivity. Although many genes in response to drought stress have been identified, genetic improvement to drought resistance especially in food crops is showing relatively slow progress worldwide. Here, we reported the isolation of abscisic acid, stress and ripening (ASR) genes from upland rice variety, IRAT109 (Oryza sativa L. ssp. japonica), and demonstrated that overexpression of OsASR5 enhanced osmotic tolerance in Escherichia coli and drought tolerance in Arabidopsis and rice by regulating leaf water status under drought stress conditions. Moreover, overexpression of OsASR5 in rice increased endogenous ABA level and showed hypersensitive to exogenous ABA treatment at both germination and postgermination stages. The production of H2O2, a second messenger for the induction of stomatal closure in response to ABA, was activated in overexpression plants under drought stress conditions, consequently, increased stomatal closure and decreased stomatal conductance. In contrast, the loss-of-function mutant, osasr5, showed sensitivity to drought stress with lower relative water content under drought stress conditions. Further studies demonstrated that OsASR5 functioned as chaperone-like protein and interacted with stress-related HSP40 and 2OG-Fe (II) oxygenase domain containing proteins in yeast and plants. Taken together, we suggest that OsASR5 plays multiple roles in response to drought stress by regulating ABA biosynthesis, promoting stomatal closure, as well as acting as chaperone-like protein that possibly prevents drought stress-related proteins from inactivation.
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Jinjie
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Guo
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Zhujia
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Zhanying
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Conghui
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Plant Systems Biotech and Crop Biotech Institute, Kyung Hee University, Yongin, Korea.
authors.forename: Gynheung
authors.initials: G
authors.lastname: An
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
authors.forename: Nam-Chon
authors.initials: NC
authors.lastname: Paek
authors.affiliation: International Rice Research Institute, Metro Manila, Philippines.
authors.forename: Jauhar
authors.initials: J
authors.lastname: Ali
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Zichao
authors.initials: Z
authors.lastname: Li
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D055864:Droughts; D004926:Escherichia coli / Q000235:genetics; D017343:Genes, Plant; D018525:Germination; D050956:HSP40 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D009154:Mutation; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D054046:Plant Stomata / Q000378:metabolism*; D030821:Plants, Genetically Modified; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D050956:HSP40 Heat-Shock Proteins; D010940:Plant Proteins; D014157:Transcription Factors; D014867:Water; D000040:Abscisic Acid; D006861:Hydrogen Peroxide
keywords: 
  ABA
  ; 
  Oryza sativa
  ; 
  OsASR5
  ; Drought; stomata; water content