title: Effects of oral calcium supplementation on mineral and acid-base status, energy metabolites, and health of postpartum dairy cows.
abstract: Two experiments were conducted to characterize blood concentrations of minerals and acid-base status after oral dosing of Ca salts and to determine the effects of oral Ca on mineral and metabolic status and incidence diseases. The hypotheses were that administration of oral Ca as CaCl2 and CaSO4 maintains blood total Ca (tCa) concentrations ≥2.125 mM and reduces the incidence of diseases in early lactation. In experiment 1, 18 Holstein cows on the day of calving were assigned to receive a single dose of 0, 43, or 86g of Ca as an oral bolus. Blood was sampled before and after treatments to characterize acid-base status and concentrations of minerals. In experiment 2, 450 Holstein cows considered of low (LRM; normal calving) or high risk (HRM; dystocia, twins, stillbirth, retained placenta, vulvo-vaginal laceration, or a combination of these) of metritis (primiparous-LRM=84; primiparous-HRM=84; multiparous-LRM=138; multiparous-HRM=138) on the day of calving were blocked by parity and then randomly assigned to control, no Ca supplementation; 86g of Ca on d 0 and 1 postpartum (CaS1); or 86g of Ca on d 0 and 1 postpartum followed by 43g/d on d 2 to 4 postpartum (CaS4). Blood was sampled before and 30 min after treatment on d 0, and 30 min after treatments on d 1 to 4, and d 7 and 10 for determination of concentrations of minerals and metabolites and blood acid-base responses. Disease incidence was evaluated for the first 30 DIM. Concentrations of ionized Ca (iCa) increased for 2h in cows supplemented with 43g of Ca and fewer than 8h in cows supplemented with 86g of Ca. The changes in iCa concentrations from pretreatment to 30 min after 86g of Ca supplemented on d 0 were 0.11±0.03 mM in multiparous cows and 0.25±0.03 mM in primiparous cows. Oral Ca reduced the incidence of subclinical hypocalcemia (SCH; tCa <2.125mM) in the first 4 d in the experiment (control=69.3%; CaS1=57.5%; CaS4=34.2%). Calcium supplementation decreased the prevalence of SCH on d 0 and 1 postpartum in all cows. Stopping oral Ca in CaS1 on d 1 postpartum, however, caused a rebound in SCH on d 2 to 4 postpartum in primiparous cows. Oral Ca increased the incidence of metritis (control=22.7%; CaS1=34.8%; CaS4=32.8%), primarily because of an increase in LRM primiparous cows (control=17.9%; CaS1=35.7%; CaS4=42.9%). Oral Ca increased morbidity in primiparous cows (control=38.1%; CaS1=61.8%; CaS4=60.3%) but had no effect on multiparous cows (control=38.2%; CaS1=35.1%; CaS4=30.1%). Large doses of oral Ca as salts of chloride and sulfate in the first days postpartum should be avoided in primiparous cows and used only in cows at risk of clinical hypocalcemia.
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville 32611.
authors.forename: N
authors.initials: N
authors.lastname: Martinez
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville 32611.
authors.forename: L D P
authors.initials: LDP
authors.lastname: Sinedino
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville 32611.
authors.forename: R S
authors.initials: RS
authors.lastname: Bisinotto
authors.affiliation: Department of Large Animal Clinical Sciences, University of Florida, Gainesville 32611.
authors.forename: R
authors.initials: R
authors.lastname: Daetz
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville 32611.
authors.forename: C
authors.initials: C
authors.lastname: Lopera
authors.affiliation: Department of Large Animal Clinical Sciences, University of Florida, Gainesville 32611.
authors.forename: C A
authors.initials: CA
authors.lastname: Risco
authors.affiliation: Department of Large Animal Clinical Sciences, University of Florida, Gainesville 32611.
authors.forename: K N
authors.initials: KN
authors.lastname: Galvão
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville 32611; DH Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville 32611.
authors.forename: W W
authors.initials: WW
authors.lastname: Thatcher
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville 32611; DH Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville 32611. Electronic address: jepsantos@ufl.edu.
authors.forename: J E P
authors.initials: JEP
authors.lastname: Santos
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000284:Administration, Oral; D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002122:Calcium Chloride / Q000008:administration & dosage / Q000097:blood; D002133:Calcium Sulfate / Q000008:administration & dosage; D002136:Calcium, Dietary / Q000008:administration & dosage* / Q000097:blood; D002417:Cattle; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D006996:Hypocalcemia / Q000097:blood / Q000175:diagnosis / Q000188:drug therapy / Q000662:veterinary; D007774:Lactation; D008274:Magnesium / Q000097:blood; D010298:Parity; D049590:Postpartum Period / Q000187:drug effects / Q000378:metabolism; D011188:Potassium / Q000097:blood; D016016:Proportional Hazards Models; D012964:Sodium / Q000097:blood; D014131:Trace Elements / Q000008:administration & dosage*; D014591:Uterine Diseases / Q000097:blood / Q000175:diagnosis / Q000188:drug therapy / Q000662:veterinary
chemical_list: D001786:Blood Glucose; D002136:Calcium, Dietary; D005230:Fatty Acids, Nonesterified; D014131:Trace Elements; D012964:Sodium; D008274:Magnesium; D002122:Calcium Chloride; D011188:Potassium; D020155:3-Hydroxybutyric Acid; D002133:Calcium Sulfate
keywords: calcium supplementation; dairy cow; metritis; subclinical hypocalcemia