title: Viability of Lucilia sericata maggots after exposure to wound antiseptics.
abstract: After debridement and before dressing a wound with maggots of calliphorid flies, one frequently performed step is the application of antiseptics to the prepared wound bed. However, the concomitant application of antiseptic agents during maggot therapy is regarded controversial as antiseptics may interfere with maggots' viability. In this experimental in vitro study, the viability of fly maggots was investigated after exposure to various antiseptics frequently used in wound care. Here, we show that Lucilia sericata fly maggots can survive up to an hour's exposure to wound antiseptics such as octenidine, povidone-iodine or polihexanide. Concomitant short-term application of wound antiseptics together with maggots on wound beds is tolerated by larvae and does not impair their viability.
authors.affiliation: Department of Dermatology, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Georg
authors.initials: G
authors.lastname: Daeschlein
authors.affiliation: Department of Trauma and Orthopedic Surgery, Clinic of Surgery, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Napp
authors.affiliation: Institute for Skin Integrity and Infection Prevention, School of Human & Health Sciences, University of Huddersfield, Huddersfield, UK.
authors.forename: Ojan
authors.identifier: http://orcid.org/0000-0003-0129-8761
authors.initials: O
authors.lastname: Assadian
authors.affiliation: Department of Dermatology, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: von Podewils
authors.affiliation: Department of Trauma and Orthopedic Surgery, Clinic of Surgery, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Kevin
authors.initials: K
authors.lastname: Reese
authors.affiliation: Department of Trauma and Orthopedic Surgery, Clinic of Surgery, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Hinz
authors.affiliation: Department for Plastic, Aesthetic and Reconstructive Surgery, Wilhelminen Hospital, Vienna, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Matiasek
authors.affiliation: Department of Trauma and Orthopedic Surgery, Clinic of Surgery, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Romy
authors.initials: R
authors.lastname: Spitzmueller
authors.affiliation: Institute for Skin Integrity and Infection Prevention, School of Human & Health Sciences, University of Huddersfield, Huddersfield, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Humphreys
authors.affiliation: Department of Dermatology, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Jünger
authors.affiliation: Institute of Hygiene and Environmental Medicine, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Kramer
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000009:adverse effects*; D002470:Cell Survival / Q000187:drug effects*; D003646:Debridement / Q000379:methods*; D006801:Humans; D007814:Larva / Q000187:drug effects* / Q000254:growth & development*; D014947:Wounds and Injuries / Q000628:therapy*
chemical_list: D000891:Anti-Infective Agents, Local
keywords: Antisepsis; Bacterial ingestion; Bio-surgery; Disinfection; Lucilia sericata; Maggot therapy; Mobility; Wound care