title: Immature Colon Carcinoma Transcript-1 (ICT1) Expression Correlates with Unfavorable Prognosis and Survival in Patients with Colorectal Cancer.
abstract: Colorectal cancer (CRC) is the third leading cause of death from cancer worldwide. Immature colon carcinoma transcript-1 (ICT1) has been reported to be correlated with lung cancer; however, whether ICT1 is a functional gene in CRC, as well as the molecular mechanism underlying ICT1 mediation of colorectal-tumor formation, remains unknown.
  
  
  
  The expression of ICT1 was firstly determined by using immunohistochemistry in 861 CRC specimens. The correlation of ICT1 expression with clinicopathological parameters and the survival rate was analyzed. Furthermore, we investigated the effect of ICT1 silencing on CRC cell proliferation and migration by MTT, colony formation, flow cytometry, and transwell in vitro.
  
  
  
  ICT1 is highly expressed in a cohort of human CRCs, and that higher ICT1 expression may lead to reduced overall survival rate of CRC. Likewise, ICT1 silencing lowered the cell viability through cell-cycle arrest, inhibited cell migration, and induced apoptosis in CRC. We further revealed a novel mechanism in which ICT1 promoted CRC growth via the intracellular AMPK, SAPK/JNK, and PARP signaling pathways.
  
  
  
  Our data showed that ICT1 could be an important target for CRC diagnosis and treatment.
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Lao
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Qingyang
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: He
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Ji
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Dexiang
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Tang
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. xujmin@aliyun.com.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. xinyu_qin@tom.com.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Qin
mesh_terms: D000263:Adenylate Kinase / Q000378:metabolism; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism* / Q000556:secondary; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D005260:Female; D059565:G2 Phase Cell Cycle Checkpoints / Q000235:genetics; D020868:Gene Silencing; D045325:HCT116 Cells; D006801:Humans; D020935:MAP Kinase Signaling System; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011379:Prognosis; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D012269:Ribosomal Proteins; D015996:Survival Rate
chemical_list: C098021:MRPL58 protein, human; D011506:Proteins; D012269:Ribosomal Proteins; D011065:Poly(ADP-ribose) Polymerases; D000263:Adenylate Kinase