title: Tepidibacillus decaturensis sp. nov., a microaerophilic, moderately thermophilic iron-reducing bacterium isolated from 1.7 km depth groundwater.
abstract: A Gram-stain-negative, microaerophilic rod-shaped organism designated as strain Z9T was isolated from groundwater of 1.7 km depth from the Mt. Simon Sandstone of the Illinois Basin, Illinois, USA. Cells of strain Z9T were rod shaped with dimensions of 0.3×(1-10) µm and stained Gram-negative. Strain Z9T grew within the temperature range 20-60 °C (optimum at 30-40 °C), between pH 5 and 8 (optimum 5.2-5.8) and under salt concentrations of 1-5 % (w/v) NaCl (optimum 2.5 % NaCl). In addition to growth by fermentation and nitrate reduction, this strain was able to reduce Fe(III), Mn(IV), Co(III) and Cr(VI) when H2 or organic carbon was available as the electron donor, but did not actively reduce oxidized sulfur compounds (e.g. sulfate, thiosulfate or S0). The G+C content of the DNA from strain Z9T was 36.1 mol%. Phylogenetic analysis of the 16S rRNA gene from strain Z9T showed that it belongs to the class Bacilli and shares 97 % sequence similarity with the only currently characterized member of the genus Tepidibacillus, T.fermentans. Based on the physiological distinctness and phylogenetic information, strain Z9T represents a novel species within the genus Tepidibacillus, for which the name Tepidibacillus decaturensis sp. nov. is proposed. The type strain is Z9T (=ATCC BAA-2644T=DSM 103037T).
authors.affiliation: Department of Geology, University of Illinois Urbana-Champaign, Urbana, IL, USA.|Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL, USA.
authors.forename: Yiran
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Geology, University of Illinois Urbana-Champaign, Urbana, IL, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Sanford
authors.affiliation: Biosciences Division, Argonne National Laboratory, Argonne, IL, USA.|Institute of Chemical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria.
authors.forename: Maxim I
authors.initials: MI
authors.lastname: Boyanov
authors.affiliation: Biosciences Division, Argonne National Laboratory, Argonne, IL, USA.
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Kemner
authors.affiliation: Biosciences Division, Argonne National Laboratory, Argonne, IL, USA.
authors.forename: Theodore M
authors.initials: TM
authors.lastname: Flynn
authors.affiliation: Biosciences Division, Argonne National Laboratory, Argonne, IL, USA.
authors.forename: Edward J
authors.initials: EJ
authors.lastname: O'Loughlin
authors.affiliation: Illinois State Geology Survey, University of Illinois Urbana-Champaign, Champaign, IL, USA.
authors.forename: Randall A
authors.initials: RA
authors.lastname: Locke
authors.affiliation: Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, IL, USA.
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Weber
authors.affiliation: Department of Biochemistry, University of Illinois Urbana-Champaign, Champaign, IL, USA.
authors.forename: Sheila M
authors.initials: SM
authors.lastname: Egan
authors.affiliation: Department of Geology, University of Illinois Urbana-Champaign, Urbana, IL, USA.|Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, IL, USA.|Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL, USA.|Illinois State Geology Survey, University of Illinois Urbana-Champaign, Champaign, IL, USA.
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Fouke
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D060587:Groundwater / Q000382:microbiology*; D007087:Illinois; D007501:Iron / Q000378:metabolism*; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D007501:Iron