title: Quantification of Rac1 and Rac1b in serum of non small cell lung cancer by label free real time assay.
abstract: BACKGROUND
  Rac proteins play a major role in tumorogenesis. We quantified Rac1 and Rac1b in serum of non small cell lung cancer (NSCLC) patients.
  
  
  METHODS
  The blood of 77 NSCLC patients and 52 healthy controls were collected and quantified the concentration of Rac1 and Rac1b mainly by surface plasmon resonance and it was verify by Western blot analysis.
  
  
  RESULTS
  Rac1 and Rac1b were found to be significantly over expressed in serum of NSCLC patients compare to healthy controls. The level of Rac proteins were found to be increased in all stages of cancer. Despite the low survival rate, we managed to collect serum sample of the 18 follow up patients after the therapy, where 11 patients' of CR+PR group showed down regulation of the Rac protein after chemotherapy and unfortunately 80% patients died during the study period.
  
  
  CONCLUSIONS
  The high specificity and sensitivity obtained from ROC analysis for Rac1 and Rac1b envisaged it to be used as a serum diagnostic marker in the early stage of cancer.
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Vishal
authors.initials: V
authors.lastname: Sahu
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Tarang
authors.initials: T
authors.lastname: Gupta
authors.affiliation: Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Anant
authors.initials: A
authors.lastname: Mohan
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India. Electronic address: sharmistha_d@hotmail.com.
authors.forename: Sharmistha
authors.initials: S
authors.lastname: Dey
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000175:diagnosis / Q000401:mortality; D016022:Case-Control Studies; D004358:Drug Therapy; D006801:Humans; D020349:Surface Plasmon Resonance; D020744:rac GTP-Binding Proteins / Q000097:blood; D020830:rac1 GTP-Binding Protein / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; C107953:RAC1 protein, human; D020744:rac GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein
keywords: NSCLC; Protein marker; SPR; Serum