title: Extraction and characterization of three polysaccharides extracted from Opuntia ficus indica cladodes.
abstract: The chemical extraction and the characterization of polysaccharides from mucilage (MC), pectin (PC) and total pectic mucilage fraction (TFC) of Opuntia ficus indica cladodes as well as the evaluation of their antioxidant activities was investigated. The FTIR spectroscopic analysis revealed the presence of carboxyl and hydroxyl groups corresponding to polysaccharides. Uronic acid and the total sugar contents of PC were higher than those of TFC and MC whereas ash content of MC was considerably more important. In addition, the findings showed that all the samples had little protein content and low average molecular weight compared to the results mentioned in literature. Furthermore, MC reached not only the highest water (WHC) and oil holding (OHC) capacities (7.81g/g and 1.34g/g, respectively) but also the highest antioxidant properties (DPPH and ABTS scavenging activities, β-carotene bleaching inhibition activity and reducing power). However, PC had the strongest emulsifying and foaming properties. As for TFC, it had low WHC, OHC and emulsifying properties whereas it had higher foaming properties than MC and greater antioxidant properties compared to PC. These outcomes can encourage the use of PC as a surfactant and MC and TFC as natural antioxidants in food and pharmaceutical industries.
authors.affiliation: Laboratory of Microrganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Road Sidi Mansour Km 6, B.P 1177 Sfax 3018, Tunisia.
authors.forename: Nadia
authors.initials: N
authors.lastname: Bayar
authors.affiliation: Laboratory of Microrganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Road Sidi Mansour Km 6, B.P 1177 Sfax 3018, Tunisia.
authors.forename: Mouna
authors.initials: M
authors.lastname: Kriaa
authors.affiliation: Laboratory of Microrganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Road Sidi Mansour Km 6, B.P 1177 Sfax 3018, Tunisia; Biotechnology High School of Sfax (ISBS) Soukra Km 3; P.O. Box 261, Sfax 3000, Tunisia. Electronic address: radhouan.kammoun@cbs.rnrt.tn.
authors.forename: Radhouane
authors.initials: R
authors.lastname: Kammoun
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D004655:Emulsions / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry; D006813:Humidity; D008970:Molecular Weight; D009821:Oils / Q000737:chemistry; D029747:Opuntia / Q000033:anatomy & histology* / Q000737:chemistry*; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification; D010940:Plant Proteins / Q000032:analysis; D011134:Polysaccharides / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000737:chemistry; D014574:Uronic Acids / Q000032:analysis; D014867:Water; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D002241:Carbohydrates; D004655:Emulsions; D016166:Free Radical Scavengers; D009821:Oils; D010851:Picrates; D010936:Plant Extracts; D010940:Plant Proteins; D011134:Polysaccharides; D013451:Sulfonic Acids; D014574:Uronic Acids; D019207:beta Carotene; D014867:Water; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Functional properties; Opuntia ficus indica cladodes; Polysaccharides