title: PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera.
abstract: We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.
authors.affiliation: Department of Biology, University of Turku, 20014 Turku, Finland; Department of Biology, Lund University, 223 62 Lund, Sweden.
authors.forename: Niklas
authors.initials: N
authors.lastname: Wahlberg
authors.affiliation: Department of Biology, University of Turku, 20014 Turku, Finland.
authors.forename: Carlos
authors.initials: C
authors.lastname: Peña
authors.affiliation: Department of Biology, University of Turku, 20014 Turku, Finland.
authors.forename: Milla
authors.initials: M
authors.lastname: Ahola
authors.affiliation: Department of Zoology, Stockholm University, 106 91 Stockholm, Sweden.
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Wheat
authors.affiliation: Department of Biology, University of Turku, 20014 Turku, Finland; Department of Biology, Lund University, 223 62 Lund, Sweden.
authors.forename: Jadranka
authors.initials: J
authors.lastname: Rota
keywords: Lepidoptera; Molecular systematics; phylogenetics; phylogenomics