title: Molecular Mechanism for Stress-Induced Depression Assessed by Sequencing miRNA and mRNA in Medial Prefrontal Cortex.
abstract: BACKGROUND
  Major depression is a prevalent mood disorder. Chronic stress is presumably main etiology that leads to the neuron and synapse atrophies in the limbic system. However, the intermediate molecules from stresses to neuronal atrophy remain elusive, which we have studied in the medial prefrontal cortices from depression mice.
  
  
  RESULTS
  The mice were treated by the chronic unpredictable mild stress (CUMS) until they expressed depression-like behaviors confirmed by the tests of sucrose preference, forced swimming and Y-maze. High-throughput sequencings of microRNA and mRNA in the medial prefrontal cortices were performed in CUMS-induced depression mice versus control mice to demonstrate the molecular profiles of major depression. In the medial prefrontal cortices of depression-like mice, the levels of mRNAs that translated the proteins for the GABAergic synapses, dopaminergic synapses, myelination, synaptic vesicle cycle and neuronal growth were downregulated. miRNAs of regulating these mRNAs are upregulated.
  
  
  CONCLUSIONS
  The deteriorations of GABAergic and dopaminergic synapses as well as axonal growth are associated with CUMS-induced depression.
authors.affiliation: Qingdao University, School of Pharmacy, Shandong, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Ma
authors.affiliation: State Key Lab of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.affiliation: College of Life Science, University of Science and Technology of China, Hefei, Anhui, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Xu
authors.affiliation: State Key Lab of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Cui
authors.affiliation: Qingdao University, School of Pharmacy, Shandong, China.|State Key Lab of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.|College of Life Science, University of Science and Technology of China, Hefei, Anhui, China.
authors.forename: Jin-Hui
authors.initials: JH
authors.lastname: Wang
mesh_terms: D000818:Animals; D003865:Major Depressive Disorder / Q000209:etiology* / Q000235:genetics* / Q000473:pathology; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D008297:Male; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics*; D017397:Prefrontal Cortex / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D013315:Stress, Psychological / Q000150:complications* / Q000235:genetics / Q000473:pathology
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger