title: Retracing the in vivo haematopoietic tree using single-cell methods.
abstract: The dynamic process by which self-renewing stem cells and their offspring proliferate and differentiate to create the erythroid, myeloid and lymphoid lineages of the blood system has long since been an important topic of study. A range of recent single cell and family tracing methodologies such as massively parallel single-cell RNA-sequencing, mass cytometry, integration site barcoding, cellular barcoding and transposon barcoding are enabling unprecedented analysis, dissection and re-evaluation of the haematopoietic tree. In addition to the substantial experimental advances, these new techniques have required significant theoretical development in order to make biological deductions from their data. Here, we review these approaches from both an experimental and inferential point of view, considering their discoveries to date, their capabilities, limitations and opportunities for further development.
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR168, Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, France.
authors.forename: Leïla
authors.initials: L
authors.lastname: Perié
authors.affiliation: Hamilton Institute, Maynooth University, Co Kildare, Ireland.
authors.forename: Ken R
authors.initials: KR
authors.lastname: Duffy
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D019070:Cell Lineage / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D022423:Myeloid Cells / Q000166:cytology / Q000378:metabolism; D059010:Single-Cell Analysis
keywords: RNA-sequencing; barcoding; haematopoietic tree; lineage tracing; mass cytometry; single cells