title: The RAB GTPase RABA1e localizes to the cell plate  and shows distinct subcellular behavior from  RABA2a under Endosidin 7 treatment.
abstract: Cytokinesis in plants requires the activity of RAB GTPases to regulate vesicle-mediated contribution of material to the developing cell plate. While some plant RAB GTPases have been shown to be involved in cell plate formation, many still await functional assignment. Here, we report cell plate localization for YFP-RABA1e in Arabidopsis thaliana and use the cytokinesis inhibitor Endosidin 7 to provide a detailed description of its localization compared to YFP-RABA2a. Differences between YFP-RABA2a and YFP-RABA1e were observed in late-stage cell plates under DMSO control treatment, and became more apparent under Endosidin 7 treatment. Taken together, our results suggest that individual RAB GTPases might make different contributions to cell plate formation and further demonstrates the utility of ES7 probe to dissect them.
authors.forename: Destiny J
authors.initials: DJ
authors.lastname: Davis
authors.forename: Stephen C
authors.initials: SC
authors.lastname: McDowell
authors.forename: Eunsook
authors.initials: E
authors.lastname: Park
authors.forename: Glenn
authors.initials: G
authors.lastname: Hicks
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Wilkop
authors.forename: Georgia
authors.initials: G
authors.lastname: Drakakaki
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000032:analysis / Q000378:metabolism* / Q000502:physiology*; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D048749:Cytokinesis / Q000187:drug effects / Q000502:physiology*; D015363:Quinolones / Q000494:pharmacology*; D015398:Signal Transduction; D020691:rab GTP-Binding Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D015363:Quinolones; C000613067:endosidin 7; C586737:Rab GTPase 11C, Arabidopsis thaliana; C000613066:RABA1e protein, Arabidopsis; D020691:rab GTP-Binding Proteins