title: A transgenic-cloned pig model expressing non-fluorescent modified Plum.
abstract: Genetically modified pigs that express fluorescent proteins such as green and red fluorescent proteins have become indispensable biomedical research tools in recent years. Cell or tissue transplantation studies using fluorescent markers should be conducted, wherein the xeno-antigenicity of the fluorescent proteins does not affect engraftment or graft survival. Thus, we aimed to create a transgenic (Tg)-cloned pig that was immunologically tolerant to fluorescent protein antigens. In the present study, we generated a Tg-cloned pig harboring a derivative of Plum modified by a single amino acid substitution in the chromophore. The cells and tissues of this Tg-cloned pig expressing the modified Plum (mPlum) did not fluoresce. However, western blot and immunohistochemistry analyses clearly showed that the mPlum had the same antigenicity as Plum. Thus, we have obtained primary proof of principle for creating a cloned pig that is immunologically tolerant to fluorescent protein antigens.
authors.affiliation: Meiji University International Institute for Bio-Resource Research, Kawasaki 214-8571, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Nagaya
authors.forename: Masahito
authors.initials: M
authors.lastname: Watanabe
authors.forename: Mirina
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Nakano
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Arai
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Asano
authors.forename: Toki
authors.initials: T
authors.lastname: Takeishi
authors.forename: Ikuma
authors.initials: I
authors.lastname: Umeki
authors.forename: Tooru
authors.initials: T
authors.lastname: Fukuda
authors.forename: Sayaka
authors.initials: S
authors.lastname: Yashima
authors.forename: Shuko
authors.initials: S
authors.lastname: Takayanagi
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Watanabe
authors.forename: Masafumi
authors.initials: M
authors.lastname: Onodera
authors.forename: Hitomi
authors.initials: H
authors.lastname: Matsunari
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Umeyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nagashima
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000941:Antigens / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D019175:DNA Methylation; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D005453:Fluorescence; D005822:Genetic Vectors; D005838:Genotype; D006085:Graft Survival; D007150:Immunohistochemistry; D049408:Luminescent Agents / Q000737:chemistry; D053652:Nuclear Transfer Techniques; D013552:Swine; D019076:Transgenes
chemical_list: D000941:Antigens; D049408:Luminescent Agents