title: Applications of a Rapid and Sensitive Dengue DUO Rapid Immunochromatographic Test Kit as a Diagnostic Strategy during a Dengue Type 2 Epidemic in an Urban City.
abstract: Dengue infection is a major health problem in tropical and subtropical countries. A prospective observational study in a university-affiliated hospital was conducted between August 2015 and September 2015. Patients who visited the emergency department (ED) with a presentation of any symptoms of dengue were eligible for the dengue non-structural protein 1 (NS1), IgM/IgG rapid immunochromatographic tests and real-time polymerase chain reaction (RT-PCR) to evaluate the performance of the rapid tests. Considering the RT-PCR as the gold standard for the dengue diagnosis, the ideal primary results of sensitivity (80-100%), specificity (60-84%), positive predicted value(75%-95%), and negative predicted value (70-100%) suggested that the NS1-based test with or without a combination of IgM and IgG tests have good diagnostic performances in detecting dengue infections, even in the afebrile or elderly populations.
authors.affiliation: Department of Emergency Medicine, National Cheng Kung University Hospital, National Cheng Kung University Tainan, Taiwan.|Department of Emergency Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.|Department of Public Health, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.|National Mosquito-Borne Diseases Control Research Center, National Health Research Institutes, Tainan, Taiwan.
authors.forename: Hsin-I
authors.initials: HI
authors.lastname: Shih
authors.affiliation: Department of Emergency Medicine, National Cheng Kung University Hospital, National Cheng Kung University Tainan, Taiwan.
authors.forename: Hsiang-Chin
authors.initials: HC
authors.lastname: Hsu
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan.|National Mosquito-Borne Diseases Control Research Center, National Health Research Institutes, Tainan, Taiwan.
authors.forename: Chi-Jung
authors.initials: CJ
authors.lastname: Wu
authors.affiliation: Department of Emergency Medicine, National Cheng Kung University Hospital, National Cheng Kung University Tainan, Taiwan.|Department of Emergency Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chih-Hao
authors.initials: CH
authors.lastname: Lin
authors.affiliation: Division of Geriatrics, Department of Internal Medicine, National Cheng Kung University Hospital, Tainan, Taiwan.|Division of Geriatrics, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chia-Ming
authors.initials: CM
authors.lastname: Chang
authors.affiliation: Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Tu
authors.affiliation: Department of Emergency Medicine, National Cheng Kung University Hospital, National Cheng Kung University Tainan, Taiwan.
authors.forename: Chih-Chia
authors.initials: CC
authors.lastname: Hsieh
authors.affiliation: Department of Emergency Medicine, National Cheng Kung University Hospital, National Cheng Kung University Tainan, Taiwan.|Department of Emergency Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chih-Hsien
authors.initials: CH
authors.lastname: Chi
authors.affiliation: Department of Health Care Management, University of Kang Ning, Tainan, Taiwan.
authors.forename: Tzu-Ching
authors.initials: TC
authors.lastname: Sung
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity / Q000379:methods*; D002947:Cities; D003715:Dengue / Q000175:diagnosis* / Q000453:epidemiology; D058872:Epidemics; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012367:RNA, Viral / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic