title: H. pylori infection is related to mitochondrial microsatellite instability in gastric carcinogenesis.
abstract: BACKGROUND
  To assess the correlation of H. pylori infection with mitochondrial microsatellite instability (mtMSI) and IL-8 in gastric carcinogenesis.
  
  
  METHODS
  H. pylori infection was evaluated through histology and a urease breath test; mtMSI was measured using PCR-single strand conformation polymorphism (PCR-SSCP); IL-8 was analyzed with ELISA methods.
  
  
  RESULTS
  The detection rate of mtMSI was significantly higher in specimens with H. pylori infection than in those without H. pylori infection (P < 0.05). The levels of IL-8 were significantly higher in specimens with mtMSI than in those without mtMSI (P < 0.01).An association of mtMSI with the intestinal histological type was found (P < 0.05). Increased IL-8 levels induced by H. pylori were related to the invasion, lymphnode spreading and clinical stage of gastric cancer (P < 0.05).
  
  
  CONCLUSIONS
  H. pylori infection is related to mitochondrial microsatellite instability in the early steps of gastric cancer development. IL-8 may play a role in the development of mtMSI induced by H. pylori. Our results support a role for mtMSI in different mechanisms of gastric carcinogenesis.
authors.affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.
authors.forename: Xianlong
authors.initials: X
authors.lastname: Ling
authors.affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.
authors.forename: Haoxiang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.
authors.forename: Caifei
authors.initials: C
authors.lastname: Shen
authors.affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.
authors.forename: Wu
authors.initials: W
authors.lastname: Yan
authors.affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.
authors.forename: Ji
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.
authors.forename: Guiyong
authors.initials: G
authors.lastname: Peng
authors.affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.
authors.forename: Dianchun
authors.initials: D
authors.lastname: Fang
keywords: Gastric cancer; H. pylori; IL-8; Mitochondrial microsatellite instability