title: Random Amplification of Polymorphic DNA Based Typing of Pseudomonas Aeruginosa.
abstract: Pseudomonas aeruginosa was isolated from various sources during the course of an epidemic outbreak of bacterial endophthalmitis following an eye camp at Sangli, Maharashtra. 15 distinct isolates were obtained from clinical samples. Typing of the 15 isolates was performed by random amplified polymorphic DNA (RAPD) analysis, pyocin typing and antibiogram. RAPD typing was rapid, labour friendly and could be done within six hours. RAPD analysis produced reproducible electrophoretic band patterns on the basis of which three distinct amplification patterns could be visualised. The conventional typing methods were labour intensive and took about 48 hours. However, the results of RAPD typing, pyocin typing and antibiogram did not correlate with each other. This study suggests that RAPD typing could be an additional rapid typing method for studying the epidemiology of infectious disease outbreaks due to P aeruginosa.
authors.affiliation: Classified Specialist (Pathology & Microbiology), Command Hospital (Air Force), Bangalore 560 007.
authors.forename: P K
authors.initials: PK
authors.lastname: Menon
authors.affiliation: Post Graduate Student, Department of Microbiology, Armed Forces Medical College, Pune - 411 040.
authors.forename: Shiva Priya
authors.initials: SP
authors.lastname: Eswaran
authors.affiliation: Chief Medical Officer, Department of Microbiology, Armed Forces Medical College, Pune - 411 040.
authors.forename: S S
authors.initials: SS
authors.lastname: Pant
authors.affiliation: Professor and Head, Department of Microbiology, BJ Medical College, Pune - 411 011.
authors.forename: R
authors.initials: R
authors.lastname: Bharadwaj
authors.affiliation: Professor and Head, Department of Microbiology, Armed Forces Medical College, Pune - 411 040.
authors.forename: A
authors.initials: A
authors.lastname: Nagendra
keywords: Antibiogram; Pseudomonas aeruginosa; Pyocin