title: Effect of maleylation on physicochemical and functional properties of rapeseed protein isolate.
abstract: Influence of maleylation on the physicochemical and functional properties of rapeseed protein isolate was studied. Acylation increased whiteness value and dissociation of proteins, but reduced free sulfhydryl and disulfide content (p < 0.05). Intrinsic fluorescence emission and FTIR spectra revealed distinct perturbations in maleylated proteins' tertiary and secondary conformations. Increase in surface hydrophobicity, foaming capacity, emulsion stability, protein surface load at oil-water interface and decrease in surface tension at air-water interface, occurred till moderate level of modification. While maleylation impaired foam stability, protein solubility and emulsion capacity were markedly ameliorated (p < 0.05), which are concomitant with decreased droplet size distribution (d 32). In-vitro digestibility and cytotoxicity tests suggested no severe ill-effects of modified proteins, especially up to low degrees of maleylation. The study shows good potential for maleylated rapeseed proteins as functional food ingredient.
authors.affiliation: Department of Food Engineering and Technology, School of Engineering, Tezpur University, Tezpur, Assam 784028 India.
authors.forename: Manashi
authors.initials: M
authors.lastname: Das Purkayastha
authors.affiliation: Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur, Assam 784028 India.
authors.forename: Anuj Kumar
authors.initials: AK
authors.lastname: Borah
authors.affiliation: Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur, Assam 784028 India.
authors.forename: Sougata
authors.initials: S
authors.lastname: Saha
authors.affiliation: Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur, Assam 784028 India.
authors.forename: Ajay Kumar
authors.initials: AK
authors.lastname: Manhar
authors.affiliation: Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur, Assam 784028 India.
authors.forename: Manabendra
authors.initials: M
authors.lastname: Mandal
authors.affiliation: Department of Food Engineering and Technology, School of Engineering, Tezpur University, Tezpur, Assam 784028 India.
authors.forename: Charu Lata
authors.initials: CL
authors.lastname: Mahanta
keywords: Cytotoxicity; Emulsification; Maleic anhydride; Rapeseed protein