title: The Genomic Sequence of the Oral Pathobiont Strain NI1060 Reveals Unique Strategies for Bacterial Competition and Pathogenicity.
abstract: Strain NI1060 is an oral bacterium responsible for periodontitis in a murine ligature-induced disease model. To better understand its pathogenicity, we have determined the complete sequence of its 2,553,982 bp genome. Although closely related to Pasteurella pneumotropica, a pneumonia-associated rodent commensal based on its 16S rRNA, the NI1060 genomic content suggests that they are different species thriving on different energy sources via alternative metabolic pathways. Genomic and phylogenetic analyses showed that strain NI1060 is distinct from the genera currently described in the family Pasteurellaceae, and is likely to represent a novel species. In addition, we found putative virulence genes involved in lipooligosaccharide synthesis, adhesins and bacteriotoxic proteins. These genes are potentially important for host adaption and for the induction of dysbiosis through bacterial competition and pathogenicity. Importantly, strain NI1060 strongly stimulates Nod1, an innate immune receptor, but is defective in two peptidoglycan recycling genes due to a frameshift mutation. The in-depth analysis of its genome thus provides critical insights for the development of NI1060 as a prime model system for infectious disease.
authors.affiliation: Department of Bioengineering Sciences, Microbiology Unit, Vrije Universiteit Brussel, Brussels, Belgium.|Center for the Biology of Disease, VIB, Leuven, Belgium.|Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, Belgium.
authors.forename: Youssef
authors.identifier: http://orcid.org/0000-0001-7655-5764
authors.initials: Y
authors.lastname: Darzi
authors.affiliation: Department of Pathology, University of Michigan, Ann Arbor, Michigan, United States of America.
authors.forename: Yizu
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Department of Pathology, University of Michigan, Ann Arbor, Michigan, United States of America.
authors.forename: Mizuho
authors.initials: M
authors.lastname: Hasegawa
authors.affiliation: Department of Pathology, University of Michigan, Ann Arbor, Michigan, United States of America.
authors.forename: Henry
authors.initials: H
authors.lastname: Moon
authors.affiliation: Department of Pathology, University of Michigan, Ann Arbor, Michigan, United States of America.|Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michigan, United States of America.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Núñez
authors.affiliation: Department of Pathology, University of Michigan, Ann Arbor, Michigan, United States of America.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Inohara
authors.affiliation: Department of Bioengineering Sciences, Microbiology Unit, Vrije Universiteit Brussel, Brussels, Belgium.|Center for the Biology of Disease, VIB, Leuven, Belgium.|Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, Belgium.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Raes
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D008070:Lipopolysaccharides / Q000737:chemistry; D015661:Pasteurellaceae / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D010518:Periodontitis / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D037521:Virulence Factors
chemical_list: D004269:DNA, Bacterial; D008070:Lipopolysaccharides; D012336:RNA, Ribosomal, 16S; D037521:Virulence Factors