title: First venom gland transcriptomic analysis of Iranian yellow scorpion "Odonthubuthus doriae" with some new findings.
abstract: Scorpion venom contains mixture of biologic molecules including selective toxins with medical capability. Odonthubuthus doriae (O. doriae) belonged to Buthidae family of scorpions and gained more interest among Iranian dangerous scorpion since 2005. We constructed the first cDNA library to explore the transcriptomic composition of this Iranian scorpiontelson. Then by used of bioinformatic software each expression sequence taq (EST) from the library analyzed and its quiddity was clear. Analysis showed that toxins (42%) had more venom transcript than other component such as antimicrobial peptides, venom peptides and cell proteins. Over 16% of transcripts didn't have any open reading frames (ORF), however their sequences showed similarity by other scorpion sequences. One EST didn't have any similarity by known scorpion peptides. For the first time; we report a comprehensive study of an Iranian scorpion with interesting and novel findings. We characterized a new putative sodium channel modifier in scorpions by some bioinformatics software, and then predicted its structure and function.
authors.affiliation: Dept. of Genetics, School of Science, ShahaidChamran University, Ahvaz, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: NaderiSoorki
authors.affiliation: Dept. of Genetics, School of Science, ShahaidChamran University, Ahvaz, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Galehdari
authors.affiliation: Dept. of Pharmacology and Toxicology, School of Pharmacy and Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: Masomeh
authors.initials: M
authors.lastname: Baradaran
authors.affiliation: Dept. of Pharmacology and Toxicology, School of Pharmacy and Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address: amjalali@hotmail.com.
authors.forename: Amir
authors.initials: A
authors.lastname: Jalali
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020869:Gene Expression Profiling; D010455:Peptides / Q000737:chemistry; D015221:Potassium Channels / Q000378:metabolism; D012604:Scorpion Venoms / Q000737:chemistry / Q000235:genetics*; D012605:Scorpions; D017386:Sequence Homology, Amino Acid; D015222:Sodium Channels / Q000378:metabolism
chemical_list: D010455:Peptides; D015221:Potassium Channels; D012604:Scorpion Venoms; D015222:Sodium Channels
keywords: Iranian scorpion; Odonthubuthus doriae; Transcriptome analysis; Venom gland; cDNA library