title: Breviscapine prevents downregulation of renal water and sodium transport proteins in response to unilateral ureteral obstruction.
abstract: OBJECTIVE
  Our recent report indicates that breviscapine play a protective role of the kidney by down-regulating transforming growth factor-β1(TGF-β1), α-smooth muscle actin (α-SMA) and alleviating interstitial fibrosis following unilateral ureteral obstruction (UUO). In this study, we investigate the effect of breviscapine on changes of renal water and sodium transport proteins in response to UUO.
  
  
  METHODS
  Male Sprague-Dawley rats were divided into 3 groups, sham group, UUO group and UUO treat with breviscapine. After 4, 7 and 14 days, histologic changes and interstitial collagen were determined microscopically following hematoxylin and eosin (H&E) and Masson's trichrome staining. The expression of Aquaporins (AQP-2) and γ-epithelial sodium channel (γ-ENaC) were investigated using immunohistochemistry and Western blot in each group.
  
  
  RESULTS
  Breviscapine treatment decrease the tubular injury index and the degree of interstitial collagen deposition significantly compared with the UUO group (P<0.05). Breviscapine treatment also significantly reduced downregulation of AQP2 and γ-ENaC compared to those subjected to the same time course of obstruction in UUO group (P<0.05).
  
  
  CONCLUSIONS
  These results demonstrate that breviscapine could prevent downregulation of renal water and sodium transport proteins in response to UUO so as to protect obstructed kidney.
authors.affiliation: Department of Intensive Care Unit, The First Affiliate Hospital of Wenzhou Medical University, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Mei
authors.affiliation: Department of Intensive Care Unit, The First Affiliate Hospital of Wenzhou Medical University, China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Yangyang
authors.affiliation: Department of Transplantation Center, The First Affiliate Hospital of Wenzhou Medical University, China.
authors.forename: Luo
authors.initials: L
authors.lastname: Shuai
authors.affiliation: Department of Transplantation Center, The First Affiliate Hospital of Wenzhou Medical University, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hao
authors.affiliation: Department of Transplantation Center, The First Affiliate Hospital of Wenzhou Medical University, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yirong
authors.affiliation: Department of Transplantation Center, The First Affiliate Hospital of Wenzhou Medical University, China.
authors.forename: Cai
authors.initials: C
authors.lastname: Yong
authors.affiliation: Department of Transplantation Center, The First Affiliate Hospital of Wenzhou Medical University, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Peng
authors.affiliation: Key Laboratory of Surgery, Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Bicheng
authors.affiliation: Department of Transplantation Center, The First Affiliate Hospital of Wenzhou Medical University, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yan
keywords: Breviscapine; Kidney; Ureteral obstruction; Water and sodium trans-; port proteins