title: Antiproliferative Scalarane-Based Metabolites from the Red Sea Sponge Hyrtios erectus.
abstract: Two new sesterterpenes analogs, namely, 12-acetoxy,16-epi-hyrtiolide (1) and 12β-acetoxy,16β-methoxy,20α-hydroxy-17-scalaren-19,20-olide (2), containing a scalarane-based framework along with seven previously reported scalarane-type sesterterpenes (3-9) have been isolated from the sponge Hyrtios erectus (order Dictyoceratida) collected from the Red Sea, Egypt. The structures of the isolated compounds were elucidated on the basis of their spectroscopic data and comparison with reported NMR data. Compounds 1-9 exhibited considerable antiproliferative activity against breast adenocarcinoma (MCF-7), colorectal carcinoma (HCT-116) and hepatocellular carcinoma cells (HepG2). Compounds 3, 5 and 9 were selected for subsequent investigations regarding their mechanism of cell death induction (differential apoptosis/necrosis assessment) and their influence on cell cycle distribution.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt. ssahmed@kau.edu.sa.|Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia. ssahmed@kau.edu.sa.
authors.forename: Sameh S
authors.initials: SS
authors.lastname: Elhady
authors.affiliation: Pharmacology Department, Medical Division, National Research Centre, Giza 12622, Egypt. ahmedmalabd@pharma.asu.edu.eg.|Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia. ahmedmalabd@pharma.asu.edu.eg.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Al-Abd
authors.affiliation: Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia. ahalawany2003@yahoo.com.|Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr el-Aini street, Cairo 11562, Egypt. ahalawany2003@yahoo.com.
authors.forename: Ali M
authors.initials: AM
authors.lastname: El-Halawany
authors.affiliation: Department of Clinical Pharmacy, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia. aalahdal2@hotmail.com.
authors.forename: Abdulrahman M
authors.initials: AM
authors.lastname: Alahdal
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt. hashem_omar@pharm.suez.edu.eg.
authors.forename: Hashim A
authors.initials: HA
authors.lastname: Hassanean
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt. safwat_aa@yahoo.com.
authors.forename: Safwat A
authors.initials: SA
authors.lastname: Ahmed
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D015179:Colorectal Neoplasms / Q000188:drug therapy; D004534:Egypt; D005260:Female; D045325:HCT116 Cells; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D011161:Porifera / Q000737:chemistry*; D054830:Sesterterpenes / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D054830:Sesterterpenes
keywords: Hyrtios erectus; Red Sea sponge; antiproliferative activity; cell based assay; scalarane framework