title: Laser desorption-ion mobility spectrometry as a useful tool for imaging of thin layer chromatography surface.
abstract: We present a novel method for coupling thin layer chromatography (TLC) with ion mobility spectrometry (IMS) using laser desorption technique (LD). After separation of the compounds by TLC, the TLC surface was sampled by the LD-IMS without any further manipulation or preparation. The position of the laser was fixed and the TLC plate was moved in desired directions by the motorized micro-positioning stage. The method was successfully applied to analyze the TLC plates containing explosives (tri nitro toluene, 1,3,5-trinitro- 1,3,5-triazacyclohexane, pentaerythritol tetranitrate, 2,4-dinitro toluene and 3,4-dinitro toluene), amino acids (alanine, proline and isoleucine), nicotine and diphenylamine mixtures and detection limits for these compounds were determined. Combination of TLC with LD-IMS technique offers additional separation dimension, allowing separation of overlapping TLC analytes. The time for TLC sampling by LD-IMS was less than 80s. The scan rate for LD is adjustable so that fast and effective analysis of the mixtures is possible with the proposed method.
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
authors.forename: Vahideh
authors.initials: V
authors.lastname: Ilbeigi
authors.affiliation: Comenius University in Bratislava, Faculty of Mathematics, Physics and Informatics, Department of Experimental Physics, Mlynska dolina, F2 842 48, Slovakia.
authors.forename: Martin
authors.initials: M
authors.lastname: Sabo
authors.affiliation: Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Younes
authors.initials: Y
authors.lastname: Valadbeigi
authors.affiliation: Comenius University in Bratislava, Faculty of Mathematics, Physics and Informatics, Department of Experimental Physics, Mlynska dolina, F2 842 48, Slovakia. Electronic address: Stefan.Matejcik@fmph.uniba.sk.
authors.forename: Stefan
authors.initials: S
authors.lastname: Matejcik
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, Iran. Electronic address: m-tabriz@cc.iut.ac.ir.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Tabrizchi
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002855:Chromatography, Thin Layer; D004159:Diphenylamine / Q000737:chemistry; D053834:Explosive Agents / Q000032:analysis*; D057230:Limit of Detection; D009538:Nicotine / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000596:Amino Acids; D053834:Explosive Agents; D009538:Nicotine; D004159:Diphenylamine
keywords: Explosives; Ion mobility spectrometry; Laser desorption; Thin layer chromatography