title: Human umbilical mesenchymal stem cells conditioned medium promote primary wound healing regeneration.
abstract: OBJECTIVE
  This research was conducted to clarify the capability of human umbilical mesenchymal stem cells conditioned medium (HU-MSCM) to promote regenerations of primary wound healing on the incision skin injury.
  
  
  METHODS
  In this study, two approaches in vitro and in vivo already done. On in vitro analysis, tube formation was performed using HU vein endothelial cells in the presence of HU-MSCM, in some experiments cells line was incubated prior the presence of lipopolysaccharide and HU-MSCM then apoptosis assay was performed. Furthermore, in vivo experiments 12 female rats (Rattus norvegicus) were used after rats anesthetized, 7 mm wound was made by incision on the left side of the body. The wound was treated with HU-MSCM containing cream, povidone iodine was run as a control. Wound healing regenerations on the skin samples were visualized by hematoxylin-eosin staining.
  
  
  RESULTS
  In vitro models elucidate HU-MSCM may decreasing inflammation at the beginning of wound healing, promote cell migration and angiogenesis. In addition in vivo models show that the incision length on the skin is decreasing and more smaller, HE staining describe decreasing of inflammation phase, increasing of angiogenesis, accelerate fibroplasia, and maturation phase.
  
  
  CONCLUSIONS
  Taken together our observation indicates that HU-MSCM could promote the acceleration of skin tissue regenerations in primary wound healing process.
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
authors.forename: Dwi Liliek
authors.initials: DL
authors.lastname: Kusindarta
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
authors.forename: Hevi
authors.initials: H
authors.lastname: Wihadmadyatami
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
authors.forename: Yuda Heru
authors.initials: YH
authors.lastname: Fibrianto
authors.affiliation: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
authors.forename: Widagdo Sri
authors.initials: WS
authors.lastname: Nugroho
authors.affiliation: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
authors.forename: Heru
authors.initials: H
authors.lastname: Susetya
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
authors.forename: Dewi Kania
authors.initials: DK
authors.lastname: Musana
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
authors.forename: Hery
authors.initials: H
authors.lastname: Wijayanto
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
authors.forename: Surya Agus
authors.initials: SA
authors.lastname: Prihatna
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
authors.forename: A E T H
authors.initials: AE
authors.lastname: Wahyuni
keywords: human umbilical mesenchymal stem cells conditioned medium; regenerations; wound healing