title: Detection and Identification of Single Molecules in Immunology and Immunohematology: Single (solution)-phase Single-molecule FCS.
abstract: In the Goodpasture experiment, we determined a probability of 99.1% of identifying one single immune complex. Under these conditions, a single molecule event is proven. There exist no instrumental assumptions of our approach on which the experiments themselves, the theoretical background or the conclusion are based on. Our results open up a broad field for analytics and diagnostics in solution, particularly in immunology and immunohematology.
authors.affiliation: a Clinical Immunology and Jean Dausset Laboratory , Graz University M.S. and Hospital , Auenbruggerplatz 8, A-8036 Graz , LKH , Austria.
authors.forename: U
authors.initials: U
authors.lastname: Demel
authors.affiliation: a Clinical Immunology and Jean Dausset Laboratory , Graz University M.S. and Hospital , Auenbruggerplatz 8, A-8036 Graz , LKH , Austria.
authors.forename: Z
authors.initials: Z
authors.lastname: Földes-Papp
authors.affiliation: a Clinical Immunology and Jean Dausset Laboratory , Graz University M.S. and Hospital , Auenbruggerplatz 8, A-8036 Graz , LKH , Austria.
authors.forename: G P
authors.initials: GP
authors.lastname: Tilz
mesh_terms: D000818:Animals; D019867:Anti-Glomerular Basement Membrane Disease / Q000276:immunology*; D001323:Autoantibodies / Q000737:chemistry* / Q000276:immunology; D001324:Autoantigens / Q000737:chemistry / Q000276:immunology*; D002232:Carbocyanines / Q000737:chemistry; D024141:Collagen Type IV / Q000737:chemistry / Q000276:immunology*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D050533:Glomerular Basement Membrane / Q000276:immunology; D006801:Humans; D051379:Mice; D012235:Rhodamines / Q000737:chemistry
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D002232:Carbocyanines; D024141:Collagen Type IV; D012235:Rhodamines; C085321:cyanine dye 5; C057112:type IV collagen alpha3 chain
keywords: Critical bulk concentration; Fluorescence correlation spectroscopy; Goodpasture autoantibodies; Poisson distribution; Single immune complex; Solution-phase single-molecule fluorescence correlation spectroscopy