title: Gene expression in term placentas is regulated more by spinal or epidural anesthesia than by late-onset preeclampsia or gestational diabetes mellitus.
abstract: Pre-eclampsia (PE) and gestational diabetes mellitus (GDM) are common complications of pregnancy, but the mechanisms underlying these disorders remain unclear. The aim was to identify the extent of altered gene expression in term placentas from pregnant women with late-onset PE and GDM compared to controls. RNAseq identified few significantly differentially regulated genes in placental biopsies between PE, GDM, or uncomplicated pregnancy (n = 10 each group). Five genes were altered in placentas from PE including 4 non-coding genes and Angiopoietin 2 (ANGPT2). No genes were significantly regulated by GDM. In contrast, many genes were significantly regulated by fetal, maternal and delivery-specific variables, particularly spinal and epidural anesthesia. We selected ANGPT2 and Chemokine (C-X-C motif) ligand 14 (CXCL14) to test with qPCR in a larger set of placentas (n = 475) and found no differences between the groups. However, regression analysis revealed a stronger association between placental ANGPT2 and CXCL14 mRNA expression and fetal, maternal and delivery-specific variables than diagnostic group. To conclude, the gene expression in term placentas are highly affected by fetal, maternal and delivery specific variables. Few regulated genes were found in late-onset PE and GDM placentas, which may suggest that these conditions could be more affected by maternal factors.
authors.affiliation: Research Institute of Internal Medicine, Oslo University Hospital, Rikshospitalet, Oslo, Norway.|Mother Infant Research Institute, Tufts Medical Center, Boston, MA, USA.
authors.forename: Tove
authors.initials: T
authors.lastname: Lekva
authors.affiliation: Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway.
authors.forename: Robert
authors.initials: R
authors.lastname: Lyle
authors.affiliation: Department of Obstetrics, Oslo University Hospital, Rikshospitalet, Oslo, Norway.
authors.forename: Marie Cecilie Paasche
authors.initials: MC
authors.lastname: Roland
authors.affiliation: Department of Obstetrics, Oslo University Hospital, Rikshospitalet, Oslo, Norway.
authors.forename: Camilla
authors.initials: C
authors.lastname: Friis
authors.affiliation: Mother Infant Research Institute, Tufts Medical Center, Boston, MA, USA.
authors.forename: Diana W
authors.initials: DW
authors.lastname: Bianchi
authors.affiliation: Molecular Cardiology Research Institute and Division of Cardiology, Department of Medicine, Tufts Medical Center, Boston, MA, USA.
authors.forename: Iris Z
authors.initials: IZ
authors.lastname: Jaffe
authors.affiliation: Mother Infant Research Institute, Tufts Medical Center, Boston, MA, USA.|Department of Obstetrics &Gynecology, Tufts Medical Center and Tufts University School of Medicine, Boston, MA, USA.
authors.forename: Errol R
authors.initials: ER
authors.lastname: Norwitz
authors.affiliation: Faculty of Medicine, University of Oslo, Oslo, Norway.|Section of Specialized Endocrinology, Department of Endocrinology, Oslo University Hospital, Rikshospitalet, Oslo, Norway.
authors.forename: Jens
authors.initials: J
authors.lastname: Bollerslev
authors.affiliation: Department of Obstetrics, Oslo University Hospital, Rikshospitalet, Oslo, Norway.
authors.forename: Tore
authors.initials: T
authors.lastname: Henriksen
authors.affiliation: Research Institute of Internal Medicine, Oslo University Hospital, Rikshospitalet, Oslo, Norway.|Faculty of Medicine, University of Oslo, Oslo, Norway.
authors.forename: Thor
authors.initials: T
authors.lastname: Ueland
mesh_terms: D000328:Adult; D000767:Anesthesia, Epidural; D000775:Anesthesia, Spinal; D016640:Diabetes, Gestational / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D011225:Pre-Eclampsia / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy