title: An internal electron reservoir enhances catalytic CO2 reduction by a semisynthetic enzyme.
abstract: The development of an artificial metalloenzyme for CO2 reduction is described. The small-molecule catalyst [Ni(II)(cyclam)](2+) has been incorporated within azurin. Selectivity for CO generation over H(+) reduction is enhanced within the protein environment, while the azurin active site metal impacts the electrochemical overpotential and photocatalytic activity. The enhanced catalysis observed for copper azurin suggests an important role for intramolecular electron transfer, analogous to native CO2 reducing enzymes.
authors.affiliation: Department of Chemistry and Biochemistry and Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA. Shafaat.1@osu.edu.
authors.forename: Camille R
authors.initials: CR
authors.lastname: Schneider
authors.affiliation: Department of Chemistry and Biochemistry and Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA. Shafaat.1@osu.edu.
authors.forename: Hannah S
authors.initials: HS
authors.lastname: Shafaat
mesh_terms: D001400:Azurin / Q000737:chemistry* / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism*; D002384:Catalysis / Q000187:drug effects; D004579:Electron Transport / Q000187:drug effects / Q000502:physiology; D004583:Electrons; D020536:Enzyme Activators / Q000737:chemistry* / Q000494:pharmacology; D017433:Protein Structure, Secondary
chemical_list: D020536:Enzyme Activators; D001400:Azurin; D002245:Carbon Dioxide