title: Renoprotective effect of a combination of garlic and telmisartan against ischemia/reperfusion-induced kidney injury in obese rats.
abstract: Obesity enhances the frequency and severity of acute kidney injury (AKI). Telmisartan pre-treatment was used experimentally in the amelioration of ischemia/reperfusion (IR)-induced AKI. However, there is a lack of evidence regarding its beneficial effects on AKI in obese animals. The present study, therefore, aimed to explore the protective effects of garlic and/or telmisartan against renal damage induced by unilateral IR in obese rats. Meloxicam was used as a standard anti-inflammatory agent. Prophylactic oral administration of meloxicam (3 mg kg(-1)), garlic (500 mg kg(-1)) and/or telmisartan (5 and 10 mg kg(-1)) for 4 wk protected against renal function deterioration induced by IR in obese rats. Both doses of telmisartan significantly reduced serum total cholesterol and triacyglycerol levels as well as peri-renal adipocytes size and renal fibrosis. Renal nuclear factor-kappa B immunoreactivity, tumor necrosis factor-alpha content as well as interleukin-10, adiponectin receptor 1 and macrophages (M1, M2) polarization markers (CD11c, CD206) mRNA expressions were down-regulated in ischemic kidney tissues and white adipose tissues around them by all treatments. Moreover, garlic, telmisartan and their combinations significantly suppressed oxidative stress in renal ischemic tissues. Histological picture was also improved by these treatments. Interestingly, the combinations provided a greater protection than their monotherapy in a dose-dependent manner. We suppose that this combination may be a promising prophylactic regimen for managing AKI in case of obesity. Thus, future experimental and clinical large-scale studies are necessary.
authors.affiliation: a Department of Biochemistry, Faculty of Pharmacy , Zagazig University , Zagazig , Sharkia Governorate , Egypt ;
authors.forename: Sousou Ibrahim
authors.initials: SI
authors.lastname: Ali
authors.affiliation: b Department of Medical Biochemistry, Faculty of Medicine , Benha University , Egypt ;
authors.forename: Naglaa Fathy
authors.initials: NF
authors.lastname: Alhusseini
authors.affiliation: a Department of Biochemistry, Faculty of Pharmacy , Zagazig University , Zagazig , Sharkia Governorate , Egypt ;
authors.forename: Hebatallah Husseini
authors.initials: HH
authors.lastname: Atteia
authors.affiliation: a Department of Biochemistry, Faculty of Pharmacy , Zagazig University , Zagazig , Sharkia Governorate , Egypt ;
authors.forename: Reham Abd El-Satar
authors.initials: RA
authors.lastname: Idris
authors.affiliation: c Department of Histology, Faculty of Medicine for Girls , Al-Azhar University , Cairo , Egypt.
authors.forename: Rehab Abdallah
authors.initials: RA
authors.lastname: Hasan
mesh_terms: D058186:Acute Kidney Injury / Q000188:drug therapy*; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D001562:Benzimidazoles / Q000008:administration & dosage / Q000627:therapeutic use*; D001565:Benzoates / Q000008:administration & dosage / Q000627:therapeutic use*; D005737:Garlic / Q000378:metabolism*; D007668:Kidney / Q000473:pathology*; D009765:Obesity / Q000150:complications*; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy*; D000077333:Telmisartan
chemical_list: D000959:Antihypertensive Agents; D001562:Benzimidazoles; D001565:Benzoates; D000077333:Telmisartan
keywords: Adiponectin receptor 1; Garlic; Inflammation; macrophages; oxidative stress; peri-renal adipocytes; renal ischemia/reperfusion injury; telmisartan