title: Design and evaluation of a novel potential carrier for a hydrophilic antitumor drug: Auricularia auricular polysaccharide-chitosan nanoparticles as a delivery system for doxorubicin hydrochloride.
abstract: To improve the low loading content of hydrophilic drugs in nanodrug delivery systems, a natural watersoluble polysaccharide, Auricularia auricular polysaccharide (AAP), was extracted and purified as a vehicle for the hydrophilic drug doxorubicin hydrochloride (Dox·HCl). This involved the preparation of polyelectrolyte complexes nanoparticles (PEC NPs) using the electrostatic interaction between cationic chitosan (CS) and anionic AAP. The formation of AAP-CS-NPs was confirmed by FT-IR and TEM. It was found that Dox-loaded AAP-CS-NPs possessed a spherical morphology with average diameters of 237.6nm and 74.1% Dox·HCl encapsulation efficiency. The stability of Dox AAP-CS-NPs was examined by suspending the nanoparticles in PBS (pH 7.4) at room temperature. The particle size of the nanoparticle samples remained stable and exhibited no obvious variations in drug content after half a month. In addition, in vitro cytotoxicity studies showed that blank AAP-CS-NPs did not exhibit any cytotoxic effects, while Dox AAP-CS-NPs increased the Dox·HCl cytotoxicity against MCF-7 cells as the result of significantly increased cellular uptake, compared with free Dox·HCl. Hence, the overall results obtained suggest that AAP-CS-NPs are very effective in entrapping Dox·HCl and to penetrate into tumor cells, rendering them promising carriers for hydrophilic antitumor drugs.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.affiliation: College of Information and Computer Engineering, Northeast Forestry University, 26 Hexing Street, Harbin 150040, China. Electronic address: hrblili@sina.com.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Comprehensive Management Office of Shenben Hospital, The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China.
authors.forename: Shenxia
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: School of Chinese Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
authors.forename: Yanyao
authors.initials: Y
authors.lastname: Liang
authors.affiliation: School of Chinese Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
authors.forename: Guogang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
authors.forename: Weisan
authors.initials: W
authors.lastname: Pan
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China; State Key Laboratory of Newtech for Chinese Medicine Pharmaceutical Progress, Shenyang 110016, China. Electronic address: yangxg123@163.com.
authors.forename: Xinggang
authors.initials: X
authors.lastname: Yang
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000302:isolation & purification; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D048271:Chitosan / Q000008:administration & dosage* / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000008:administration & dosage*; D004337:Drug Carriers / Q000008:administration & dosage / Q000302:isolation & purification; D016503:Drug Delivery Systems / Q000379:methods*; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000008:administration & dosage*; D011134:Polysaccharides / Q000008:administration & dosage* / Q000302:isolation & purification
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D011134:Polysaccharides; D004317:Doxorubicin; D048271:Chitosan
keywords: Auricularia auricular polysaccharide; Chitosan; Doxorubicin hydrochloride; Hydrophilic; Nanoparticles