title: Expression of a repressor form of the Arabidopsis thaliana transcription factor TCP16 induces the formation of ectopic meristems.
abstract: Plants that express a fusion of the Arabidopsis thaliana class I TCP transcription factor TCP16 to the EAR repressor domain develop several phenotypic alterations, including rounder leaves, short petioles and pedicels, and delayed elongation of sepals, petals and anthers. In addition, these plants develop lobed cotyledons and ectopic meristems. Ectopic meristems are formed on the adaxial side of cotyledon petioles and arise from a cleft that is formed at this site. Analysis of the expression of reporter genes indicated that meristem genes are reactivated at the site of emergence of ectopic meristems, located near the bifurcation of cotyledon veins. The plants also show increased transcript levels of the boundary-specific CUP-SHAPED COTYLEDON (CUC) genes. The results suggest that TCP16 is able to modulate the induction of meristematic programs and the differentiation state of plant cells.
authors.affiliation: Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000, Santa Fe, Argentina.
authors.forename: Nora G
authors.initials: NG
authors.lastname: Uberti-Manassero
authors.affiliation: Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000, Santa Fe, Argentina.
authors.forename: Ezequiel R
authors.initials: ER
authors.lastname: Coscueta
authors.affiliation: Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000, Santa Fe, Argentina. Electronic address: dhgonza@fbcb.unl.edu.ar.
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Gonzalez
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D018548:Cotyledon / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D018519:Meristem / Q000235:genetics* / Q000254:growth & development; D059828:Plant Cells / Q000502:physiology; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C426007:CUC1 protein, Arabidopsis; C478975:CUC3 protein, Arabidopsis; D011994:Recombinant Proteins; D012097:Repressor Proteins; C513135:TCP16 protein, Arabidopsis; D014157:Transcription Factors
keywords: Arabidopsis thaliana; EAR domain; Ectopic meristem; Plant development; TCP domain; Transcription factor