title: Knock out of the PHOSPHATE 2 Gene TaPHO2-A1 Improves Phosphorus Uptake and Grain Yield under Low Phosphorus Conditions in Common Wheat.
abstract: MiR399 and its target PHOSPHATE2 (PHO2) play pivotal roles in phosphate signaling in plants. Loss of function mutation in PHO2 leads to excessive Pi accumulation in shoots and growth retardation in diploid plants like Arabidopsis thaliana and rice (Oryza sativa). Here we isolated three PHO2 homologous genes TaPHO2-A1, -B1 and -D1 from hexaploid wheat (Triticum aestivum). These TaPHO2 genes all contained miR399-binding sites and were able to be degraded by tae-miR399. TaPHO2-D1 was expressed much more abundantly than TaPHO2-A1 and -B1. The ion beam-induced deletion mutants were used to analyze the effects of TaPHO2s on phosphorus uptake and plant growth. The tapho2-a1, tapho2-b1 and tapho2-d1 mutants all had significant higher leaf Pi concentrations than did the wild type, with tapho2-d1 having the strongest effect, and tapho2-b1 the weakest. Two consecutive field experiments showed that knocking out TaPHO2-D1 reduced plant height and grain yield under both low and high phosphorus conditions. However, knocking out TaPHO2-A1 significantly increased phosphorus uptake and grain yield under low phosphorus conditions, with no adverse effect on grain yield under high phosphorus conditions. Our results indicated that TaPHO2s involved in phosphorus uptake and translocation, and molecular engineering TaPHO2 shows potential in improving wheat yield with less phosphorus fertilizer.
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Sciences, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ouyang
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Sciences, Chinese Academy of Sciences, Beijing 100101, China.|Taizhou Academy of Agricultural Sciences, Linhai, Zhejiang 317000, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Hong
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Sciences, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xueqiang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Sciences, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Sciences, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xue
authors.initials: X
authors.lastname: He
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Sciences, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Ma
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Sciences, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Teng
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Sciences, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Tong
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D001665:Binding Sites; D002523:Edible Grain / Q000235:genetics* / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000235:genetics; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics* / Q000378:metabolism; D014908:Triticum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; C000601386:MIRN399 microRNA, Arabidopsis; D035683:MicroRNAs; D010710:Phosphates; D020033:Protein Isoforms; D010758:Phosphorus; C514696:UBC24 protein, Arabidopsis; D044763:Ubiquitin-Conjugating Enzymes