title: Molecular characterization, expression and methylation status analysis of BMP4 gene in skin tissue of Liaoning cashmere goat during hair follicle cycle.
abstract: Bone morphogenetic protein 4 (BMP4) is a member of the bone morphogenetic protein family (BMPs). It is involved in the development and cycle of hair follicle, as well as, is thought to be a potential candidate gene for cashmere traits in goats. In the present study, we isolated and characterized a full-length open reading frame (ORF) of BMP4 cDNA from the skin tissue of Liaoning cashmere goat, and investigated the transcriptional pattern and methylation status of BMP4 gene in skin tissue of this breed during different stages of hair follicle cycle. The sequence analysis indicated that the isolated cDNA was 1264-bp in length containing a complete ORF of 1230-bp. It encoded a precursor peptide of 409 amino acids with a signal peptide of 19 amino acids. The structural analysis indicated that goat BMP4 contains typical TGF-β propeptide and TGF-β domains. In skin tissue, BMP4 is generally transcribed in an ascendant pattern from anagen to telogen. The methylation level of 5' flanking regulatory region of BMP4 gene might be involved in its mRNA expression in skin tissue: a higher BMP4 methylation level in skin coincides with a lower expression of BMP4 mRNA. These results from the present work provided a foundation for further insight into the functional and regulatory characteristics of BMP4 in the development and cycle of hair follicle in Liaoning Cashmere goat.
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
authors.forename: Wen L
authors.initials: WL
authors.lastname: Bai
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
authors.forename: Yun L
authors.initials: YL
authors.lastname: Dang
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
authors.forename: Jiao J
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China. yinronghuan@163.com.
authors.forename: Rong H
authors.initials: RH
authors.lastname: Yin
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
authors.forename: Ze Y
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
authors.forename: Yu B
authors.initials: YB
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
authors.forename: Yu Y
authors.initials: YY
authors.lastname: Cong
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
authors.forename: Hui L
authors.initials: HL
authors.lastname: Xue
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Deng
authors.affiliation: Institute of Animal Husbandry Science of Liaoning Province, 130062, Liaoyang, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Guo
authors.affiliation: Institute of Animal Husbandry Science of Liaoning Province, 130062, Liaoyang, China.
authors.forename: Shi Q
authors.initials: SQ
authors.lastname: Wang
authors.affiliation: Institute of Animal Husbandry Science of Liaoning Province, 130062, Liaoyang, China.
authors.forename: Shu H
authors.initials: SH
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D055415:Bone Morphogenetic Protein 4 / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018899:CpG Islands; D019175:DNA Methylation; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D006041:Goats / Q000235:genetics*; D018859:Hair Follicle / Q000378:metabolism*; D008958:Models, Molecular; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D017422:Sequence Analysis, DNA; D012867:Skin / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D055415:Bone Morphogenetic Protein 4; D018076:DNA, Complementary
keywords: BMP4 gene; Liaoning cashmere goat; Methylation; Skin tissue