title: Neutralizing Antibodies against Plasmodium falciparum Associated with Successful Cure after Drug Therapy.
abstract: An effective antibody response can assist drug treatment to contribute to better parasite clearance in malaria patients. To examine this, sera were obtained from two groups of adult patients with acute falciparum malaria, prior to drug treatment: patients who (1) have subsequent recrudescent infection, or (2) were cured by Day 28 following treatment. Using a Plasmodium falciparum antigen library, we examined the antibody specificities in these sera. While the antibody repertoire of both sera groups was extremely broad and varied, there was a differential antibody profile between the two groups of sera. The proportion of cured patients with antibodies against EXP1, MSP3, GLURP, RAMA, SEA and EBA181 was higher than the proportion of patients with recrudescent infection. The presence of these antibodies was associated with higher odds of treatment cure. Sera containing all six antibodies impaired the invasion of P. falciparum clinical isolates into erythrocytes. These results suggest that antibodies specific against EXP1, MSP3, GLURP, RAMA, SEA and EBA181 in P. falciparum infections could assist anti-malarial drug treatment and contribute to the resolution of the malarial infection.
authors.affiliation: Singapore Immunology Network, Agency for Science, Technology and Research, Singapore, Singapore.
authors.forename: Yun Shan
authors.initials: YS
authors.lastname: Goh
authors.affiliation: Singapore Immunology Network, Agency for Science, Technology and Research, Singapore, Singapore.
authors.forename: Kaitian
authors.initials: K
authors.lastname: Peng
authors.affiliation: Singapore Immunology Network, Agency for Science, Technology and Research, Singapore, Singapore.|Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Wan Ni
authors.initials: WN
authors.lastname: Chia
authors.affiliation: School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Anthony
authors.initials: A
authors.lastname: Siau
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Kesinee
authors.initials: K
authors.lastname: Chotivanich
authors.affiliation: Singapore Immunology Network, Agency for Science, Technology and Research, Singapore, Singapore.
authors.forename: Anne-Charlotte
authors.initials: AC
authors.lastname: Gruner
authors.affiliation: School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Peter
authors.initials: P
authors.lastname: Preiser
authors.affiliation: Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Mahosot Hospital, Vientiane, Laos.
authors.forename: Mayfong
authors.initials: M
authors.lastname: Mayxay
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Sasithon
authors.initials: S
authors.lastname: Pukrittayakamee
authors.affiliation: Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Mae Sot, Thailand.
authors.forename: Kanlaya
authors.initials: K
authors.lastname: Sriprawat
authors.affiliation: Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Mae Sot, Thailand.|Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
authors.forename: Francois
authors.initials: F
authors.lastname: Nosten
authors.affiliation: Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.|Mahidol Oxford Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: White
authors.affiliation: Singapore Immunology Network, Agency for Science, Technology and Research, Singapore, Singapore.|School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Laurent
authors.identifier: http://orcid.org/0000-0003-0349-1557
authors.initials: L
authors.lastname: Renia
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D057134:Antibodies, Neutralizing / Q000096:biosynthesis* / Q000097:blood; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000097:blood; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000097:blood / Q000235:genetics / Q000276:immunology; D000962:Antimalarials / Q000627:therapeutic use*; D000077549:Artemether; D037621:Artemisinins / Q000627:therapeutic use; D017963:Azithromycin / Q000627:therapeutic use; D015331:Cohort Studies; D004912:Erythrocytes / Q000187:drug effects / Q000469:parasitology; D004983:Ethanolamines / Q000627:therapeutic use; D005260:Female; D005449:Fluorenes / Q000627:therapeutic use; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D056724:Immunity, Humoral; D000078102:Lumefantrine; D016778:Malaria, Falciparum / Q000097:blood / Q000188:drug therapy* / Q000276:immunology* / Q000469:parasitology; D008297:Male; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D012008:Recurrence; D016896:Treatment Outcome
chemical_list: D057134:Antibodies, Neutralizing; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000962:Antimalarials; D037621:Artemisinins; C474029:EBA-181 protein, Plasmodium falciparum; D004983:Ethanolamines; D005449:Fluorenes; D007106:Immune Sera; D015800:Protozoan Proteins; C063362:QF116 antigen, Plasmodium falciparum; C089133:merozoite surface protein 3, Plasmodium; C072492:glutamate-rich protein, Plasmodium; D017963:Azithromycin; D000077549:Artemether; D000078102:Lumefantrine