title: New biological potential of abietane diterpenoids isolated from Salvia austriaca against microbial virulence factors.
abstract: The increasing importance of multi-resistant strains and microbial biofilms in the development of chronic infections has driven the search for more effective alternative therapy including plant-origin preparations. The present study evaluates the broadly-defined antimicrobial activity of two abietane diterpenoids isolated from Salvia austriaca transformed roots: taxodone and 15-deoxy-fuerstione. The direct biostatic/biocidal effect of these phytocompounds and their influence on Staphylococcus aureus and Candida albicans virulence factors/mechanisms (adhesion, biofilm formation, agglutination in human plasma, survival in the blood, germ tube and mycelium formation) were tested using in vitro assays. Both phytocompounds significantly inhibited microbial adhesion and biofilm formation when used at ½ and ¼ MIC. Additionally, taxodone was able to limit staphylococcal survival in human blood, as well as C. albicans germ tube formation and hyphal growth. The tested diterpenoids express significant anti-biofilm activity against both staphylococci and yeast, and adversely affect their virulence factors/mechanisms, which are relevant in the course of the infection in vivo. Therefore, they demonstrate considerable biomedical potential as complements for classic therapy with antibiotics.
authors.affiliation: Laboratory of Infectious Biology, Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland. Electronic address: bsad@biol.uni.lodz.pl.
authors.forename: Beata
authors.initials: B
authors.lastname: Sadowska
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Kuźma
authors.affiliation: Laboratory of Infectious Biology, Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
authors.forename: Bartłomiej
authors.initials: B
authors.lastname: Micota
authors.affiliation: Laboratory of Infectious Biology, Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Budzyńska
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Wysokińska
authors.affiliation: Laboratory of Molecular Spectroscopy, Department of Chemistry, University of Lodz, Tamka 12, 91-403 Lodz, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Kłys
authors.affiliation: Laboratory of Infectious Biology, Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Więckowska-Szakiel
authors.affiliation: Laboratory of Infectious Biology, Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Różalska
mesh_terms: D045784:Abietanes / Q000302:isolation & purification / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology; D025301:Hyphae / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D064209:Phytochemicals / Q000302:isolation & purification / Q000494:pharmacology*; D027544:Salvia / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology; D037521:Virulence Factors / Q000037:antagonists & inhibitors*
chemical_list: D045784:Abietanes; D000890:Anti-Infective Agents; D064209:Phytochemicals; D037521:Virulence Factors
keywords: Abietane diterpenoids; Biofilm; Candida albicans; Staphylococcus aureus; Virulence factors