title: HIF-1-Dependent TGM1 Expression is Associated with Maintenance of Airway Epithelial Junction Proteins.
abstract: Hypoxia has been implicated in the pathogenesis of many inflammatory and fibrotic lung diseases. The effect of hypoxia on epithelial junction protein expression is yet to be fully elucidated but evidence suggests a protective role for the hypoxia-inducible transcription factor HIF-1 in stabilising occludin. Transglutaminase 1 (TGM1) has been shown to stabilise endothelial and keratinocyte cell junctions, and while its expression and function have been mostly studied in the skin, recent studies have reported its expression in the lung. We hypothesised that TGM1 is a hypoxia-induced regulator of pulmonary epithelial junction protein stability, and the aim of this study was to investigate the regulation of TGM1 expression by hypoxia.
  
  
  
  Hypoxia-responsive genes were identified in human small airway epithelial cells (SAECs) by DNA microarray. TGM1 mRNA expression in SAECs was measured by quantitative real-time PCR. Protein expression of TGM1 and junction proteins was investigated by western blotting. Hypoxia-induced TGM1 was analysed by immunohistochemistry in vivo. The TGM1 gene promoter was investigated by luciferase assay.
  
  
  
  In vitro exposure of SAECs to hypoxia induced a significant increase in TGM1 expression at both mRNA and protein levels. TGM1 was also significantly upregulated in hypoxic mouse lung epithelium. The hypoxia-responsive region was mapped to a HIF-1-responsive element. Inhibition of HIF-1 expression abolished hypoxia-induced promoter activation. Overexpression of TGM1 in lung epithelial cells or exposure of SAECs to hypoxia led to upregulated expression of junction proteins.
  
  
  
  Herein we report that TGM1 is a HIF-1-regulated gene that is associated with the upregulation of airway epithelial junction proteins, supporting a protective role for HIF-1 in the lung. Interventions that augment the expression of TGM1 may provide useful therapeutic strategies for maintaining pulmonary epithelial integrity during lung injury.
authors.affiliation: UCD Conway Institute for Biomolecular and Biomedical Research, School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Lili
authors.initials: L
authors.lastname: Li
authors.affiliation: UCD Conway Institute for Biomolecular and Biomedical Research, School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland.|Centre for Experimental Medicine, Queen's University Belfast, Wellcome-Wolfson Building, Belfast, Northern Ireland, UK.
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Watson
authors.affiliation: UCD Conway Institute for Biomolecular and Biomedical Research, School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Mickael
authors.initials: M
authors.lastname: Dubourd
authors.affiliation: UCD Conway Institute for Biomolecular and Biomedical Research, School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Aine
authors.initials: A
authors.lastname: Bruton
authors.affiliation: UCD Conway Institute for Biomolecular and Biomedical Research, School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Maojia
authors.initials: M
authors.lastname: Xu
authors.affiliation: UCD Conway Institute for Biomolecular and Biomedical Research, School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Gordon
authors.initials: G
authors.lastname: Cooke
authors.affiliation: UCD Conway Institute for Biomolecular and Biomedical Research, School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland. john.baugh@ucd.ie.
authors.forename: John A
authors.initials: JA
authors.lastname: Baugh
mesh_terms: D000072283:A549 Cells; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D015687:Cell Hypoxia; D004847:Epithelial Cells; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D000860:Hypoxia / Q000235:genetics* / Q000378:metabolism; D051793:Hypoxia-Inducible Factor 1 / Q000235:genetics*; D008297:Male; D051379:Mice; D062793:Occludin / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000378:metabolism; D011503:Transglutaminases / Q000235:genetics* / Q000378:metabolism*; D015854:Up-Regulation; D062826:Zonula Occludens-1 Protein / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015820:Cadherins; D051793:Hypoxia-Inducible Factor 1; D062793:Occludin; D012333:RNA, Messenger; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; D051176:beta Catenin; D011503:Transglutaminases; C105804:transglutaminase 1
keywords: Epithelial barrier; HIF-1; Junction proteins; TGM1