title: Detection of biosynthetic gene and phytohormone production by endophytic actinobacteria associated with Solanum lycopersicum and their plant-growth-promoting effect.
abstract: In the present study, fifteen endophytic actinobacterial isolates recovered from Solanum lycopersicum were studied for their antagonistic potential and plant-growth-promoting (PGP) traits. Among them, eight isolates showed significant antagonistic and PGP traits, identified by amplification of the 16S rRNA gene. Isolate number DBT204, identified as Streptomyces sp., showed multiple PGP traits tested in planta and improved a range of growth parameters in seedlings of chili (Capsicum annuum L.) and tomato (S. lycopersicum L.). Further, genes of indole acetic acid (iaaM) and 1-aminocyclopropane-1-carboxylate (ACC) deaminase (acdS) were successively amplified from five strains. Six antibiotics (trimethoprim, fluconazole, chloramphenicol, nalidixic acid, rifampicin and streptomycin) and two phytohormones [indole acetic acid (IAA) and kinetin (KI)] were detected and quantified in Streptomyces sp. strain DBT204 using UPLC-ESI-MS/MS. The study indicates the potential of these PGP strains for production of phytohormones and shows the presence of biosynthetic genes responsible for production of secondary metabolites. It is the first report showing production of phytohormones (IAA and KI) by endophytic actinobacteria having PGP and biosynthetic potential. We propose Streptomyces sp. strain DBT204 for inoculums production and development of biofertilizers for enhancing growth of chili and tomato seedlings.
authors.affiliation: Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University, Aizawl, Mizoram 796004, India.
authors.forename: Ajit Kumar
authors.initials: AK
authors.lastname: Passari
authors.affiliation: SAIF, CSIR-Central Drug Research Institute (CSIR-CDRI), Lucknow 226012, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Chandra
authors.affiliation: Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University, Aizawl, Mizoram 796004, India.
authors.lastname: Zothanpuia
authors.affiliation: Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University, Aizawl, Mizoram 796004, India.
authors.forename: Vineet Kumar
authors.initials: VK
authors.lastname: Mishra
authors.affiliation: Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University, Aizawl, Mizoram 796004, India.
authors.forename: Vincent Vineeth
authors.initials: VV
authors.lastname: Leo
authors.affiliation: Molecular Glyco-Biotechnology Group, Discipline of Biochemistry, National University of Ireland Galway, Galway, Ireland.
authors.forename: Vijai Kumar
authors.initials: VK
authors.lastname: Gupta
authors.affiliation: SAIF, CSIR-Central Drug Research Institute (CSIR-CDRI), Lucknow 226012, India.
authors.forename: Brijesh
authors.initials: B
authors.lastname: Kumar
authors.affiliation: Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University, Aizawl, Mizoram 796004, India. Electronic address: bhimpratap@gmail.com.
authors.forename: Bhim Pratap
authors.initials: BP
authors.lastname: Singh
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism; D002212:Capsicum / Q000254:growth & development / Q000382:microbiology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000254:growth & development* / Q000382:microbiology*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S
keywords: 1-Aminocyclopropane-1-carboxylate (ACC) deaminase; Antagonistic traits, PGP; Endophytic actinobacteria; Solanum lycopersicum; UPLC-ESI-MS/MS; iaaM