title: Mannose-binding Lectin Mediated Complement Pathway in Autoimmune Neurological Disorders.
abstract: Multiple sclerosis (MS) is a complex, demyelinating disease of the central nervous system (CNS) with variable phenotypic presentations, while Guillain-Barre Syndrome (GBS) is the prototypic acute inflammatory disorder that affects the peripheral nervous system. Myasthenia gravis (MG) is a T cell dependent and antibody mediated autoimmune disease. Although it has been shown that complement plays a critical role in the pathogenesis of MS, GBS, and MG, the role of mannose-binding lectin (MBL) as a biomarker of immunopathogensis of these diseases and also its association with the severity of them have been poorly investigated. Therefore, in this study we aimed to measure plasma levels of MBL in patients with MS, GBS, and MG. In a case-control study, plasma was obtained from healthy controls (n=100) and also patients with MS (n=120), GBS (n=30), and MG (n=30). Plasma level measurement of MBL was performed using enzyme-linked immunosorbent assay (ELISA). The mean serum level of MBL was significantly different between groups of patients and healthy controls (p<0.001). We also found a positive correlation between plasma levels of MBL and severity scores of MS, MG, and GBS patients including: expanded disability status scale (EDSS) (r=+0.60 and p=<0.001), quantitative myasthenia gravis score (QMGS) (r=+0.56 and p=0.01), and GBS disability scale (GDS) (r=+0.37 and p=0.04). Taken together, our findings suggest that complement activation mediated by MBL contributes to the pathogenesis and also severity of MS, MG, and GBS. However, because the lectin pathway can be involved in several phases of the immune response, further evidence will be required to elucidate the underlying mechanism.
authors.affiliation: Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Farrokhi
authors.affiliation: Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mehrnoosh
authors.initials: M
authors.lastname: Dabirzadeh
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Nastaran
authors.initials: N
authors.lastname: Dastravan
authors.affiliation: Multiple Sclerosis and Neuroimmunology Research Center, Isfahan, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Etemadifar
authors.affiliation: Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Keyvan
authors.initials: K
authors.lastname: Ghadimi
authors.affiliation: Department of Radiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Saadatpour
authors.affiliation: Department of Radiology, School of Medicine, Najafabad University of Medical Sciences, Isfahan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Rezaei
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D037582:Complement Pathway, Mannose-Binding Lectin / Q000276:immunology*; D005260:Female; D020275:Guillain-Barre Syndrome / Q000097:blood / Q000276:immunology*; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood / Q000276:immunology*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology*; D009157:Myasthenia Gravis / Q000097:blood / Q000276:immunology*
chemical_list: D037601:Mannose-Binding Lectin
keywords: Autoimmunity; Complement system proteins; Guillain-Barre syndrome; Mannose-binding lectin; Multiple sclerosis; Myasthenia gravis