title: Bacteriocinogenic Bacteria Isolated from Raw Goat Milk and Goat Cheese Produced in the Center of México.
abstract: Currently, there are few reports on the isolation of microorganisms from goat milk and goat cheese that have antibacterial activity. In particular, there are no reports on the isolation of microorganisms with antibacterial activity from these products in central Mexico. Our objective was to isolate bacteria, from goat products, that synthesized antimicrobial peptides with activity against a variety of clinically significant bacteria. We isolated and identified Lactobacillus rhamnosus, L. plantarum, L. pentosus, L. helveticus and Enterococcus faecium from goat cheese, and Aquabacterium fontiphilum, Methylibium petroleiphilum, Piscinobacter aquaticus and Staphylococcus xylosus from goat milk. These bacteria isolated from goat cheese were able to inhibit Staphylococcus aureus, Bacillus cereus, Escherichia coli, Listeria monocytogenes, L. inoccua, Pseudomona aeruginosa, Shigella flexneri, Serratia marcescens, Enterobacter cloacae and Klebsiella pneumoniae. In addition, bacteria from goat milk showed inhibitory activity against B. cereus, L. lactis, E. coli, S. flexneri, E. cloacae and K. pneumonia; S. aureus, L. innocua, S. agalactiae and S. marcescens. The bacteriocins produced by these isolates were shown to be acid stable (pH 2-6) and thermotolerant (up to 100 °C), but were susceptible to proteinases. When screened by PCR for the presence of nisin, pediocin and enterocin A genes, none was found in isolates recovered from goat milk, and only the enterocin A gene was found in isolates from goat cheese.
authors.affiliation: División de Ciencias de la Vida, Universidad de Guanajuato, Campus Irapuato-Salamanca (CIS), Ex-Hacienda El Copal km. 9, carretera Irapuato-Silao; A.P. 311, C.P. 36500 Irapuato, Guanajuato Mexico ; Graduate Program in Biosciences, Life Science Division, Universidad de Guanajuato, Ex-Hacienda El Copal km. 9, carretera Irapuato-Silao; A.P. 311, C.P. 36500 Irapuato, Guanajuato Mexico.
authors.forename: Oscar F
authors.initials: OF
authors.lastname: Hernández-Saldaña
authors.affiliation: División de Ciencias de la Vida, Universidad de Guanajuato, Campus Irapuato-Salamanca (CIS), Ex-Hacienda El Copal km. 9, carretera Irapuato-Silao; A.P. 311, C.P. 36500 Irapuato, Guanajuato Mexico ; Departamento de Agronomía, Universidad de Guanajuato CIS, Ex-Hacienda El Copal km. 9, carretera Irapuato-Silao; A.P. 311, C.P. 36500 Irapuato, Guanajuato Mexico ; Graduate Program in Biosciences, Life Science Division, Universidad de Guanajuato, Ex-Hacienda El Copal km. 9, carretera Irapuato-Silao; A.P. 311, C.P. 36500 Irapuato, Guanajuato Mexico.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Valencia-Posadas
authors.affiliation: Escuela de Ciencias Biológicas, Universidad Autónoma de Coahuila, Boulevard Torreón-Matamoros Km. 7.5, Ciudad Universitaria Campus Torreón, C.P. 27104 Torreón, Coahuila Mexico.
authors.forename: Norma M
authors.initials: NM
authors.lastname: de la Fuente-Salcido
authors.affiliation: Department of Natural and Mathematical Sciences, California Baptist University, 8432 Magnolia Avenue, Riverside, CA 92504 USA ; Department of Entomology, University of California, Riverside, Riverside, CA 92521 USA.
authors.forename: Dennis K
authors.initials: DK
authors.lastname: Bideshi
authors.affiliation: División de Ciencias de la Vida, Universidad de Guanajuato, Campus Irapuato-Salamanca (CIS), Ex-Hacienda El Copal km. 9, carretera Irapuato-Silao; A.P. 311, C.P. 36500 Irapuato, Guanajuato Mexico ; Departamento de Alimentos, Universidad de Guanajuato CIS, Ex-Hacienda El Copal km. 9, carretera Irapuato-Silao; A.P. 311, C.P. 36500 Irapuato, Guanajuato Mexico ; Graduate Program in Biosciences, Life Science Division, Universidad de Guanajuato, Ex-Hacienda El Copal km. 9, carretera Irapuato-Silao; A.P. 311, C.P. 36500 Irapuato, Guanajuato Mexico.
authors.forename: José E
authors.initials: JE
authors.lastname: Barboza-Corona
keywords: Bacteriocins; Enterocin A; Goat cheese; Goat milk; Pathogenic bacteria