title: Genetic effect of an A/G polymorphism in the HSP70 gene on thermotolerance in chicken.
abstract: Studying thermotolerance is important for the prevention of thermostress in chickens. This study aimed to analyze the effect of mutations in the heat shock protein 70 (HSP70) gene on chicken thermotolerance. The C.-69A>G SNP in the 5'-flanking region of the HSP70 gene was genotyped in Lingshan and White Recessive Rock (WRR) chickens. Association of this SNP with thermotolerance traits revealed it to be significantly associated with CD4+/CD8+, and potentially associated with heterophil-to-lymphocyte ratio in WRR chickens exposed to thermoneutral temperature (15°C). Online prediction detected a putative myeloid zinc finger protein 1 binding factor in the C.-69A>G mutation. Under acute thermostress, mRNA levels of HSP70 in individuals with different C.-69A>G genotypes varied in the heart, leg muscle, and liver tissues. The HSP70 protein was expressed at higher levels in individuals with the GG genotype than in those with the AA genotype. In heart and liver, protein expression of HSP70 in individuals with the GG genotype was significantly higher than in those with the AA genotype. In leg muscle, protein expression was higher in birds with the GG genotype than in those with the AA and AG genotypes. Luciferase activity of the GG genotype was significantly higher than that of the AA genotype, suggesting that the C.-69A>G SNP regulates HSP70 gene expression. These results indicate that the C.-69A>G SNP in the 5'-flanking region of the HSP70 gene might affect chicken thermotolerance and that the GG genotype might be advantageous for the prevention of thermostress.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, China.|Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Chen
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, China.|Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: W W
authors.initials: WW
authors.lastname: Zhang
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, China.|Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: J K
authors.initials: JK
authors.lastname: Gan
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, China.|Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: L N
authors.initials: LN
authors.lastname: Kong
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, China.|Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, China.|Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: D X
authors.initials: DX
authors.lastname: Zhang
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, China.|Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: Q B
authors.initials: QB
authors.lastname: Luo
mesh_terms: D024506:5' Flanking Region; D000818:Animals; D002645:Chickens / Q000235:genetics / Q000502:physiology*; D005260:Female; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000378:metabolism; D000071436:Thermotolerance / Q000235:genetics*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger