title: Identification of saponins from sugar beet (Beta vulgaris) by low and high-resolution HPLC-MS/MS.
abstract: We profiled triterpene saponins from the roots of sugar beet Beta vulgaris L. cultivars Huzar and Boryna using reversed-phase liquid chromatography combined with negative-ion electrospray ionization quadrupole mass spectrometry. We tentatively identified 26 triterpene saponins, including 17 that had not been detected previously in this plant species and 7 saponins that were tentatively identified as new compounds. All observed compounds were glycosides of five different aglycones, of which gypsogenin and norhederagenin are reported for the first time in sugar beet. Thirteen of the saponins detected in sugar beet roots were substituted with dioxolane-type (4 saponins) or acetal-type (9 saponins) dicarboxylic acids. Among the 26 detected saponins, we identified 2 groups of isomers distinguished using high-resolution mass measurements that were detected only in the Huzar cultivar of sugar beet.
authors.affiliation: Department of Natural Science and Quality Assurance, Faculty of Commodity Science, Poznań University of Economics and Business, Al. Niepodległości 10, 61-875 Poznań, Poland. Electronic address: katarzyna.mikolajczyk-bator@ue.poznan.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Mikołajczyk-Bator
authors.affiliation: Department of Natural Science and Quality Assurance, Faculty of Commodity Science, Poznań University of Economics and Business, Al. Niepodległości 10, 61-875 Poznań, Poland.
authors.forename: Alfred
authors.initials: A
authors.lastname: Błaszczyk
authors.affiliation: Department of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics of the Polish Academy of Sciences, 34 Strzeszyńska, 60-479 Poznań, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Czyżniejewski
authors.affiliation: Department of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics of the Polish Academy of Sciences, 34 Strzeszyńska, 60-479 Poznań, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Kachlicki
mesh_terms: D027461:Beta vulgaris / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D007536:Isomerism; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D012503:Saponins
keywords: Akebonoic acid; Gypsogenin; HPLC–MS/MS; Hederagenin; Norhederagenin; Oleanolic acid; Sugar beet (Beta vulgaris L.); Triterpene saponins