title: Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis.
abstract: Trichomoniasis caused by Trichomonas vaginalis is a common sexually transmitted disease. Its association with several health problems, including preterm birth, pelvic inflammatory disease, cervical cancer, and transmission of human immunodeficiency virus, emphasizes the importance of improved access to early and accurate detection of T. vaginalis. In this study, a rapid and efficient loop-mediated isothermal amplification-based method for the detection of T. vaginalis was developed and validated, using vaginal swab specimens from subjects suspected to have trichomoniasis. The LAMP assay targeting the actin gene was highly sensitive with detection limits of 1 trichomonad and 1 pg of T. vaginalis DNA per reaction, and specifically amplified the target gene only from T. vaginalis. Validation of this assay showed that it had the highest sensitivity and better agreement with PCR (used as the gold standard) compared to microscopy and multiplex PCR. This study showed that the LAMP assay, targeting the actin gene, could be used to diagnose early infections of T. vaginalis. Thus, we have provided an alternative molecular diagnostic tool and a point-of-care test that may help to prevent trichomoniasis transmission and associated complications.
authors.affiliation: Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu 41944, Korea.
authors.forename: Youn-Kyoung
authors.initials: YK
authors.lastname: Goo
authors.affiliation: Department of Obstetrics and Gynecology, Shinsegae Women's Hospital, Daegu 41535, Korea.
authors.forename: Won-Sik
authors.initials: WS
authors.lastname: Shin
authors.affiliation: Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu 41944, Korea.
authors.forename: Hye-Won
authors.initials: HW
authors.lastname: Yang
authors.affiliation: Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu 41944, Korea.
authors.forename: So-Young
authors.initials: SY
authors.lastname: Joo
authors.affiliation: Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu 41944, Korea.
authors.forename: Su-Min
authors.initials: SM
authors.lastname: Song
authors.affiliation: Department of Environmental Biology & Medical Parasitology, Hanyang University College of Medicine, Seoul 04763, Korea.
authors.forename: Jae-Sook
authors.initials: JS
authors.lastname: Ryu
authors.affiliation: Department of Parasitology, Dong-A University College of Medicine, Busan 49201, Korea.
authors.forename: Hyun-Hee
authors.initials: HH
authors.lastname: Kong
authors.affiliation: Center of Biostatistics, Kyungpook National University School of Medicine, Daegu 41944, Korea.
authors.forename: Won-Ki
authors.initials: WK
authors.lastname: Lee
authors.affiliation: Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu 41944, Korea.
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Chung
authors.affiliation: Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu 41944, Korea.
authors.forename: Yeonchul
authors.initials: Y
authors.lastname: Hong
mesh_terms: D000199:Actins / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D005260:Female; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity; D014247:Trichomonas Vaginitis / Q000175:diagnosis* / Q000469:parasitology; D014246:Trichomonas vaginalis / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000199:Actins; D016054:DNA, Protozoan
keywords: PCR; Trichomonas vaginalis; loop-mediated isothermal amplification; multiplex PCR; trichomoniasis