title: Analysis of the Trypanosoma brucei EATRO 164 Bloodstream Guide RNA Transcriptome.
abstract: The mitochondrial genome of Trypanosoma brucei contains many cryptogenes that must be extensively edited following transcription. The RNA editing process is directed by guide RNAs (gRNAs) that encode the information for the specific insertion and deletion of uridylates required to generate translatable mRNAs. We have deep sequenced the gRNA transcriptome from the bloodstream form of the EATRO 164 cell line. Using conventionally accepted fully edited mRNA sequences, ~1 million gRNAs were identified. In contrast, over 3 million reads were identified in our insect stage gRNA transcriptome. A comparison of the two life cycle transcriptomes show an overall ratio of procyclic to bloodstream gRNA reads of 3.5:1. This ratio varies significantly by gene and by gRNA populations within genes. The variation in the abundance of the initiating gRNAs for each gene, however, displays a trend that correlates with the developmental pattern of edited gene expression. A comparison of related major classes from each transcriptome revealed a median value of ten single nucleotide variations per gRNA. Nucleotide variations were much less likely to occur in the consecutive Watson-Crick anchor region, indicating a very strong bias against G:U base pairs in this region. This work indicates that gRNAs are expressed during both life cycle stages, and that differential editing patterns observed for the different mitochondrial mRNA transcripts are not due to the presence or absence of gRNAs. However, the abundance of certain gRNAs may be important in the developmental regulation of RNA editing.
authors.affiliation: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America.
authors.forename: Laura E
authors.initials: LE
authors.lastname: Kirby
authors.affiliation: Department of Computer Science and Engineering, Michigan State University, East Lansing, Michigan, United States of America.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Merial Veterinary Scholars Program, Michigan State University, East Lansing, Michigan, United States of America.
authors.forename: David
authors.initials: D
authors.lastname: Judah
authors.affiliation: Department of Entomology, Michigan State University, East Lansing, Michigan, United States of America.
authors.forename: Scooter
authors.initials: S
authors.lastname: Nowak
authors.affiliation: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America.
authors.forename: Donna
authors.initials: D
authors.lastname: Koslowsky
mesh_terms: D001483:Base Sequence; D005786:Gene Expression Regulation / Q000502:physiology; D054629:Genome, Mitochondrial; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan