title: Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat.
abstract: OBJECTIVE
  To estimate genetic variability in exon 3 of caprine myostatin gene in Marwari goats.
  
  
  METHODS
  A total of 120 blood samples from unrelated Marwari goats were randomly collected from different villages of Bikaner (Rajasthan), India. Genomic DNA was extracted from whole blood using blood DNA isolation kit (Himedia Ltd.) as per manufacturer's protocol. The quality of extracted genomic DNA was checked on 0.8% agarose gel. Specifically designed a primer set for caprine myostatin (MSTN) gene (Genebank accession no. DQ167575) was used to amplify the exon 3 region of MSTN gene in Marwari goat. The genetic variability in exon 3 of MSTN gene in Marwari goat was assessed on 8% polyacrylamide gel electrophoresis to detect single strand conformation polymorphism (SSCP) pattern.
  
  
  RESULTS
  The exon 3 of MSTN gene in Marwari goat showed two types of conformation patterns on 8% polyacrylamide gel. One of the patterns showed only two bands and was considered as genotype AA, whereas another pattern having an extra band was designated as genotype AB. The frequencies of AA and AB genotype for exon 3 region of MSTN gene were calculated as 0.90 and 0.10, respectively.
  
  
  CONCLUSIONS
  Low level of polymorphism was observed at exon 3 region of MSTN gene in Marwari goat through SSCP analysis. This information could be utilized in future breeding plan to exploit the unique characteristics of Marwari goat of Rajasthan.
authors.affiliation: Molecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India.
authors.forename: Jai Prakash
authors.initials: JP
authors.lastname: Khichar
authors.affiliation: Molecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India.
authors.forename: Gyan Chand
authors.initials: GC
authors.lastname: Gahlot
authors.affiliation: Molecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India.
authors.forename: Vijay Kumar
authors.initials: VK
authors.lastname: Agrawal
authors.affiliation: Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, College of Veterinary and Animal Science, Bikaner, Rajasthan, India.
authors.lastname: Kiran
authors.affiliation: Molecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India.
authors.forename: Ajay Singh
authors.initials: AS
authors.lastname: Dewna
authors.affiliation: Molecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India.
authors.lastname: Prakash
authors.affiliation: Molecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ashraf
keywords: Marwari goat; exon 3; myostatin gene; polymerase chain reaction-single strand conformation polymorphism