title: Enantiomeric separation of triacylglycerols containing polyunsaturated fatty acids with 18 carbon atoms.
abstract: Regioisomers and enantiomers of triacylglycerols (TAGs) containing any combination of stearidonic (18:4n-3) and octadecapentaenoic (18:5n-3) acids were prepared by organic synthesis. Gradient polar organic liquid chromatography/high resolution atmospheric pressure chemical ionization-tandem mass spectrometry (NARP-LC/HRMS2-APCI) and chiral liquid chromatography were used for the separation and identification of molecular species of these TAGs. Further, NARP-LC and chiral LC were used to separate natural mixtures of TAGs obtained from the haptophyte alga Coccolithophora sp. cultivated in a salinity range from 7.5 to 60‰. The ratio of regioisomers and enantiomers was found to change with increasing salinity of the culture medium. This can be explained by variable activity of acyltransferases in cells exposed to salt stress.
authors.affiliation: Institute of Microbiology, CAS, Vídeňská 1083, 142 20 Prague, Czech Republic. Electronic address: rezanka@biomed.cas.cz.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Řezanka
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Ecology, Viničná 7, 128 44 Prague, Czech Republic.
authors.forename: Linda
authors.initials: L
authors.lastname: Nedbalová
authors.affiliation: Institute of Microbiology, CAS, Vídeňská 1083, 142 20 Prague, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Sigler
mesh_terms: D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid; D015525:Fatty Acids, Omega-3 / Q000737:chemistry / Q000302:isolation & purification; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry; D014280:Triglycerides / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D014280:Triglycerides; C062895:stearidonic acid
keywords: Alga; Chiral chromatography; Chiral triacylglycerols; Coccolithophora; Enantiomers; Haptophyte; Regioisomers; Tandem mass spectrometry