title: Qualitative and quantitative analysis of specific polysaccharides in Dendrobium huoshanense by using saccharide mapping and chromatographic methods.
abstract: Qualitative and quantitative analysis of specific polysaccharides from ten batches of Dendrobium huoshanense were performed using high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector (HPSEC-MALLS-RID), gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR) and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and high performance thin layer chromatography (HPTLC). Results showed that molecular weights, the radius of gyrations, and contents of specific polysaccharides in D. huoshanense were ranging from 1.16×10(5) to 2.17×10(5)Da, 38.8 to 52.1nm, and 9.9% to 19.9%, respectively. Furthermore, the main monosaccharide compositions were Man and Glc. Indeed, the main glycosidic linkages were β-1,4-Manp and β-1,4-Glcp, and substituted with acetyl groups at O-2 and O-3 of 1,4-linked Manp. Moreover, results showed that PACE and HPTLC fingerprints of partial acidic and enzymatic hydrolysates of specific polysaccharides were similar, which are helpful to better understand the specific polysaccharides in D. huoshanense and beneficial to improve their quality control. These approaches could also be routinely used for quality control of polysaccharides in other medicinal plants.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.
authors.forename: Ling-Xiao
authors.initials: LX
authors.lastname: Chen
authors.affiliation: Anhui Collaborative Innovation Center of Dendrobium Industrialization, Lu'an, Anhui, PR China; College of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, Anhui, PR China.
authors.forename: Bang-Xing
authors.initials: BX
authors.lastname: Han
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.
authors.forename: Ding-Tao
authors.initials: DT
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.
authors.forename: Kit-Leong
authors.initials: KL
authors.lastname: Cheong
authors.affiliation: Anhui Collaborative Innovation Center of Dendrobium Industrialization, Lu'an, Anhui, PR China; College of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, Anhui, PR China. Electronic address: cnf505@126.com.
authors.forename: Nai-Fu
authors.initials: NF
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China. Electronic address: zhaojing.cpu@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China; Anhui Collaborative Innovation Center of Dendrobium Industrialization, Lu'an, Anhui, PR China; College of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, Anhui, PR China. Electronic address: lishaoping@hotmail.com.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Li
mesh_terms: D002850:Chromatography, Gel / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D031667:Dendrobium / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D011134:Polysaccharides
keywords: Dendrobium huoshanense; HPSEC-MALLS-RID; PACE; Saccharide mapping; Specific polysaccharides