title: Molecular Cloning of Secreted Luciferases from Marine Planktonic Copepods.
abstract: Secreted luciferases isolated from copepod crustaceans are frequently used for nondisruptive reporter-gene assays, such as the continuous, automated and/or high-throughput monitoring of gene expression in living cells. All known copepod luciferases share highly conserved amino acid residues in two similar, repeated domains in the sequence. The similarity in the domains are ideal nature for designing PCR primers to amplify cDNA fragments of unidentified copepod luciferases from bioluminescent copepod crustaceans. Here, we introduce how to establish a cDNA encoding novel copepod luciferases from a copepod specimen by PCR with degenerated primers.
authors.affiliation: Department of Diabetes and Endocrinology, Saitama Medical University, 38 Morohongo, Moroyama, Iruma-gun, Saitama, 350-0495, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takenaka
authors.affiliation: Center for Information Biology, National Institute of Genetics, Yata 1111, Mishima, Shizuoka, 411-8540, Japan.
authors.forename: Kazuho
authors.initials: K
authors.lastname: Ikeo
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan. yasushi.shigeri@aist.go.jp.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shigeri
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D003062:Codon; D033342:Copepoda / Q000235:genetics*; D015870:Gene Expression; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism
chemical_list: D003062:Codon; D008156:Luciferases
keywords: Copepod; GLuc; Luciferase; MLuc; MpLuc; Plankton