title: TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology.
abstract: Whether a mixed type of craniopharyngioma (CP) exists and whether papillary craniopharyngioma (pCP) is on a histopathological continuum with Rathke's cleft cyst (RCC) remain controversial. Herein, we examined the expression and localization of β-catenin, BRAF p.V600E (V600E), and triggering receptor expressed on myeloid cells-1 (TREM-1) in 58 samples including 20 pCPs, 26 adamantinomatous craniopharyngiomas (aCP), and 12 RCCs. Five aCPs were diagnosed with mixed type CPs and the remaining 21 cases were pure aCPs. Four of the 12 RCCs presented with significant squamous epithelium (SE). V600E immunoreactivity was observed in all pCPs in the cytoplasm, but not in the nuclei. aCPs and RCCs, including mixed type CP, did not express V600E. Nuclear β-catenin translocation was detected exclusively in aCPs. TREM-1 was expressed in pCPs. Additionally, TREM-1 expression was detected in the SE of 5 "mixed type" CPs, while it was absent in pure aCPs. TREM-1 was expressed in 4 RCCs with SE, but not in the remaining 8 RCCs. TREM-1 mRNA levels were compared in cultured pCP and aCP cells. TREM-1 mRNA level was significantly (p < 0.001; up to 4.045 fold) higher in pCPs than in aCPs. Western blotting revealed a significantly (p < 0.001; up to 7.19 fold) lower level of TREM-1 expression in aCP cells compared to that in pCP cells. Our findings further supported that RCC and pCP may represent two ends of a morphological spectrum. A variant showing overlapping histological features of aCP and pCP should not be considered as a mixed type.
authors.affiliation: Department of Neurosurgery, Nanfang Hospital of Southern Medical University, Guangzhou, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Chao-Hu
authors.initials: CH
authors.lastname: Wang
authors.affiliation: Department of Biometric, College of Public Health of Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Dan-Ling
authors.initials: DL
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Nanfang Hospital of Southern Medical University, Guangzhou, China.
authors.forename: Shi-Chao
authors.initials: SC
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Nanfang Hospital of Southern Medical University, Guangzhou, China.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Peng
authors.affiliation: Department of Neurosurgery, Nanfang Hospital of Southern Medical University, Guangzhou, China.
authors.forename: Jun-Xiang
authors.initials: JX
authors.lastname: Peng
authors.affiliation: Department of Neurosurgery, Nanfang Hospital of Southern Medical University, Guangzhou, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Neurosurgery, Nanfang Hospital of Southern Medical University, Guangzhou, China.
authors.forename: Song-Tao
authors.initials: ST
authors.lastname: Qi
authors.affiliation: Department of Neurosurgery, Nanfang Hospital of Southern Medical University, Guangzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Pan
mesh_terms: D000293:Adolescent; D000328:Adult; D002467:Cell Nucleus / Q000378:metabolism; D020863:Central Nervous System Cysts / Q000378:metabolism*; D002648:Child; D003397:Craniopharyngioma / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004252:DNA Mutational Analysis; D005091:Exons; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010911:Pituitary Neoplasms / Q000378:metabolism; D048493:Proto-Oncogene Proteins B-raf / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D000073871:Triggering Receptor Expressed on Myeloid Cells-1 / Q000378:metabolism*; D055815:Young Adult; D051176:beta Catenin / Q000378:metabolism
chemical_list: C495270:CTNNB1 protein, human; D012333:RNA, Messenger; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D051176:beta Catenin; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf
keywords: Rathke’s cleft cyst; SE; TREM-1; craniopharyngioma; metaplasia