title: Strontium mineralization of shark vertebrae.
abstract: Determining the age of sharks using vertebral banding is a vital component of management, but the causes of banding are not fully understood. Traditional shark ageing is based on fish otolith ageing methods where growth bands are assumed to result from varied seasonal calcification rates. Here we investigate these assumptions by mapping elemental distribution within the growth bands of vertebrae from six species of sharks representing four different taxonomic orders using scanning x-ray fluorescence microscopy. Traditional visual growth bands, determined with light microscopy, were more closely correlated to strontium than calcium in all species tested. Elemental distributions suggest that vertebral strontium bands may be related to environmental variations in salinity. These results highlight the requirement for a better understanding of shark movements, and their influence on vertebral development, if confidence in age estimates is to be improved. Analysis of shark vertebrae using similar strontium-focused elemental techniques, once validated for a given species, may allow more successful estimations of age on individuals with few or no visible vertebral bands.
authors.affiliation: Department of Biological Sciences, Macquarie University, Sydney NSW 2109, Australia.
authors.forename: Vincent
authors.initials: V
authors.lastname: Raoult
authors.affiliation: Fisheries NSW, Sydney Institute of Marine Science, 19 Chowder Bay Road, Mosman NSW 2088, Australia.
authors.forename: Victor M
authors.initials: VM
authors.lastname: Peddemors
authors.affiliation: ANSTO Life Sciences, Lucas Heights, NSW 2234, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Zahra
authors.affiliation: ANSTO Life Sciences, Lucas Heights, NSW 2234, Australia.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Howell
authors.affiliation: Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria 3168, Australia.
authors.forename: Daryl L
authors.initials: DL
authors.lastname: Howard
authors.affiliation: Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria 3168, Australia.
authors.forename: Martin D
authors.initials: MD
authors.lastname: de Jonge
authors.affiliation: Department of Biological Sciences, Macquarie University, Sydney NSW 2109, Australia.
authors.forename: Jane E
authors.initials: JE
authors.lastname: Williamson
mesh_terms: D000365:Age Determination by Skeleton; D000818:Animals; D002113:Calcification, Physiologic; D002118:Calcium / Q000378:metabolism; D005260:Female; D008297:Male; D012754:Sharks / Q000254:growth & development / Q000378:metabolism*; D013052:Spectrometry, X-Ray Emission; D013131:Spine / Q000254:growth & development / Q000378:metabolism*; D013324:Strontium / Q000378:metabolism*
chemical_list: D002118:Calcium; D013324:Strontium