title: Study of the Effect of Route of Administration of Mesenchymal Stem Cells on Cisplatin-Induced Acute Kidney Injury in Sprague Dawley Rats.
abstract: OBJECTIVE
  Mesenchymal stem cells (MSCs) have been shown to ameliorate cisplatin-induced acute kidney injury (AKI). The present study compares the efficacy of different routes of MSCs administration on kidney damage and regeneration after cisplatin-induced AKI.
  
  
  METHODS
  A single intraperitoneal injection of cisplatin (5 mg/kg) was used to induce AKI in 160 rats. MSCs (5×10⁶) were given by either intravenous, intra-arterial or kidney sub capsular injection one day after cisplatin injection. Suitable control groups were included. Rats were sacrificed at 4, 7, 11 and 30 days after cisplatin injection. Kidney function parameters, kidney tissue oxidative stress markers, and scoring for renal tissue injury, regeneration and chronicity were all determined.
  
  
  RESULTS
  MSCs by any routes were able to ameliorate kidney function deterioration and renal tissue damage induced by cisplatin. The overall results of the three routes were equal. Differences between the different routes in one parameter were transient and inconsistent with other parameters.
  
  
  CONCLUSIONS
  Changing the route of MSCs injection does not have a major influence on the outcome. Future evaluation should focus on differences between the routes of administration considering the long term safety.
authors.affiliation: Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Fatma E
authors.initials: FE
authors.lastname: Moustafa
authors.affiliation: Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Mohamed-A
authors.initials: MA
authors.lastname: Sobh
authors.affiliation: Department of Clinical Pharmacology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abouelkheir
authors.affiliation: Mansoura Medical Experimental Research Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Youmna
authors.initials: Y
authors.lastname: Khater
authors.affiliation: Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Khalid
authors.initials: K
authors.lastname: Mahmoud
authors.affiliation: Department of Clinical Pharmacology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Mohamed-Ahdy
authors.initials: MA
authors.lastname: Saad
authors.affiliation: Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt.|Mansoura Medical Experimental Research Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Sobh
keywords: Acute kidney injury; Cisplatin; Mesenchymal stem cells; Routes of administration; Sub capsular