title: Adventitious match probability for autosomal profiles when primer binding site mutation is possible.
abstract: This paper considers the situation where two DNA systems with differing primers have been used to produce DNA profiles for loading and searching of a DNA Database. With any profiling system there exists the possibility of a "primer binding site mutation" (PBSM). When such a mutation occurs at one of the loci in a profile, it has the effect that the associated allele is not visible in the profile. In the case where a person has two different alleles at a given locus (heterozygous) the effect of a PBSM would be that the profile would appear to be that of an individual with only one allele at that locus (homozygous). The paper investigates the potential for an adventitious match as a result of a PBSM when, for example, a crime profile and person profile that have originated from two different individuals are found to be the same as a result of a PBSM in one of the profiles. It is demonstrated, both by theory and using simulations, that the effect of PBSMs is to slightly decrease the adventitious match probability from what it would had the same DNA system been used.
authors.affiliation: Principal Forensic Services, Melbury House, 34 Southborough Road, Bickley, Bromley, Kent BR1 2EB, UK. Electronic address: suepope@principalforensicservices.com.
authors.forename: Susan
authors.initials: S
authors.lastname: Pope
authors.affiliation: Principal Forensic Services, Melbury House, 34 Southborough Road, Bickley, Bromley, Kent BR1 2EB, UK.
authors.forename: Ian
authors.initials: I
authors.lastname: Evett
authors.affiliation: LGC, 3 Drayton Manor Business Park, Tamworth B78 3GL, UK.
authors.forename: Roberto
authors.initials: R
authors.lastname: Puch-Solis
mesh_terms: D001665:Binding Sites; D016172:DNA Fingerprinting; D017931:DNA Primers; D030561:Databases, Nucleic Acid; D006801:Humans; D009154:Mutation; D011336:Probability
chemical_list: D017931:DNA Primers
keywords: Adventitious match; DNA database; PCR chemistry; Primer binding site mutation