title: Preparation and Assay of Simple Light off Biosensor Based on Immobilized Bioluminescent Bacteria for General Toxicity Assays.
abstract: We describe a simple light off bioluminescent biosensor for general environmental toxicity assays based on immobilized bioluminescent bacteria engineered with beetle luciferases.
authors.affiliation: Laboratory of Biochemistry and Biotechnology of Bioluminescence, Department of Physics, Chemistry and Mathematics, Federal University of São Carlos (UFSCar), Sorocaba, SP, Brazil.|Graduate School of Biotechnology and Environmental Monitoring, Federal University of São Carlos (UFSCar), il. João Leme dos Santos Road, SP-264, Km 110, Sorocaba, SP, Brazil.|Graduate School of Evolutive Genetics and Molecular Biology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.
authors.forename: G V M
authors.initials: GV
authors.lastname: Gabriel
authors.affiliation: Laboratory of Biochemistry and Biotechnology of Bioluminescence, Department of Physics, Chemistry and Mathematics, Federal University of São Carlos (UFSCar), Sorocaba, SP, Brazil. viviani@ufscar.br.|Graduate School of Biotechnology and Environmental Monitoring, Federal University of São Carlos (UFSCar), il. João Leme dos Santos Road, SP-264, Km 110, Sorocaba, SP, Brazil. viviani@ufscar.br.|Graduate School of Evolutive Genetics and Molecular Biology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil. viviani@ufscar.br.
authors.forename: V R
authors.initials: VR
authors.lastname: Viviani
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D015374:Biosensing Techniques; D015870:Gene Expression; D017930:Genes, Reporter; D006863:Hydrogen-Ion Concentration; D008027:Light; D049409:Luciferases, Firefly / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D050296:Microbial Viability / Q000187:drug effects; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D049409:Luciferases, Firefly
keywords: Bioluminescent biosensor; Toxicity biosensor; pH-sensitive firefly luciferase