title: Mechanistically harvesting of Chlorella vulgaris and Rhodotorula glutinis via modified montmorillonoid.
abstract: In this study, the flocculation process of Chlorella vulgaris and Rhodotorula glutinis induced by inorganic salts modified montmorillonoid was conducted. The maximum flocculation efficiency (FE) of 98.50% for C. vulgaris and 11.83% for R. glutinis were obtained with 4g/L and 5g/L flocculant within the dosage scope of 1-5g/L. The difference of FE was then thermodynamically explained by the extended DLVO theory and the FE of R. glutinis was mechanically enhanced to 90.66% with 0.06g/L cationic polyacrylamide (CPAM) at an optimum pH of 9. After that, aimed to utilize the remainder flocculant capacity, C. vulgaris culture was added to the aggregation of R. glutinis. Fortunately, the coagulation of R. glutinis and C. Vulgaris was achieved with 0.05g/L CPAM and 5g/L flocculant at pH 9 and the FE reached 90.15% and 91.24%, respectively.
authors.affiliation: Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: zhangxu@mail.buct.edu.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D056804:Biofuels; D002412:Cations; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D048408:Chlorella vulgaris / Q000737:chemistry* / Q000254:growth & development; D005427:Flocculation; D012248:Rhodotorula / Q000737:chemistry* / Q000254:growth & development; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D056804:Biofuels; D002412:Cations; D000099166:Polyacrylamides
keywords: Coagulation; Extended DLVO theory; Flocculation efficiency; Oleaginous strains