title: The Yiqi and Yangyin Formula ameliorates injury to the hematopoietic system induced by total body irradiation.
abstract: In this study, we examined whether the Yiqi and Yangyin Formula (YYF), used in traditional Chinese medicine, could ameliorate damage to the hematopoietic system induced by total body irradiation (TBI). Treatment with 15 g/kg of YYF increased the survival rate of Institute of Cancer Research (ICR) mice exposed to 7.5 Gy TBI. Furthermore, YYF treatment increased the white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB) and hematocrit (HCT) counts in ICR mice exposed to 2 Gy or 4 Gy TBI. Treatment with YYF also increased the number of bone marrow cells, hematopoietic progenitor cells (HPCs), hematopoietic stem cells (HSCs) and the colony-forming ability of granulocyte-macrophage cells. YYF alleviated TBI-induced suppression of the differentiation ability of HPCs and HSCs and decreased the reactive oxygen species (ROS) levels in bone marrow mononuclear cells (BMMNCs), HPCs and HSCs from mice exposed to 2 Gy or 4 Gy TBI. Overall, our data suggest that YYF can ameliorate myelosuppression by reducing the intracellular ROS levels in hematopoietic cells after TBI at doses of 2 Gy and 4 Gy.
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China.
authors.forename: Junling
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China.|Department of Hematology and Oncology, the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China.|Department of Hematology and Oncology, the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Hematology and Oncology, the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Hematology and Oncology, the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Zhexin
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China lideguan@irm-cams.ac.cn.
authors.forename: Deguan
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000528:radiation effects; D002452:Cell Count; D000066673:Cell Self Renewal / Q000187:drug effects / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000473:pathology* / Q000528:radiation effects; D008813:Mice, Inbred ICR; D017382:Reactive Oxygen Species / Q000378:metabolism; D016019:Survival Analysis; D014916:Whole-Body Irradiation
chemical_list: D004365:Drugs, Chinese Herbal; D017382:Reactive Oxygen Species; C548799:yiqi yangyin
keywords: Yiqi and Yangyin Formula; hematopoietic system; ionizing irradiation; reactive oxygen species