title: A Single Fluorescent Probe to Visualize Hydrogen Sulfide and Hydrogen Polysulfides with Different Fluorescence Signals.
abstract: Hydrogen sulfide (H2 S) and hydrogen polysulfides (H2 Sn , n>1) are endogenous regulators of many physiological processes. In order to better understand the symbiotic relationship and cellular cross-talk between H2 S and H2 Sn , it is highly desirable to develop single fluorescent probes which enable dual-channel discrimination between H2 S and H2 Sn . Herein, we report the rational design, synthesis, and evaluation of the first dual-detection fluorescent probe DDP-1 that can visualize H2 S and H2 Sn with different fluorescence signals. The probe showed high selectivity and sensitivity to H2 S and H2 Sn in aqueous media and in cells.
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA, 99164, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA, 99164, USA.
authors.forename: Armando
authors.initials: A
authors.lastname: Pacheco
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, 113-0033, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takano
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA, 99164, USA.
authors.forename: Jacob J
authors.initials: JJ
authors.lastname: Day
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, 113-0033, Japan.
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA, 99164, USA. mxian@wsu.edu.
authors.forename: Ming
authors.identifier: 0000-0002-7902-2987
authors.initials: M
authors.lastname: Xian
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D013440:Sulfides / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D013440:Sulfides; C032915:polysulfide; D006862:Hydrogen Sulfide
keywords: cyclization; fluorescence; fluorescent probes; hydrogen polysulfides; sulfides