title: The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i.
abstract: Calcium-imaging techniques were used to determine if mouse retinal astrocytes in situ respond to agonists of ionotropic (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, AMPA; N-methyl-D-aspartate, NMDA) and metabotropic (S-3,5-dihydroxyphenylglycine, DHPG; trans-1-amino-1,3-cyclopentanedicarboxylic acid, ACPD) glutamate receptors. In most cases we found no evidence that retinal astrocyte intracellular calcium ion concentration ([Ca(2+)]i) increased in response to these glutamate agonists. The one exception was AMPA that increased [Ca(2+)]i in some, but not all, mouse retinal astrocytes in situ. However, AMPA did not increase [Ca(2+)]i in mouse retinal astrocytes in vitro, suggesting that the effect of AMPA in situ may be indirect.
authors.affiliation: Retina and Optic Nerve Research Laboratory, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2; Department of Medical Neuroscience, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2.
authors.forename: Stephanie N
authors.initials: SN
authors.lastname: Blandford
authors.affiliation: Retina and Optic Nerve Research Laboratory, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2; Department of Medical Neuroscience, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2; Department of Ophthalmology & Visual Sciences, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2.
authors.forename: William H
authors.identifier: 0000-0002-1847-8147
authors.initials: WH
authors.lastname: Baldridge
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D016257:Fura-2 / Q000378:metabolism; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000494:pharmacology; D008810:Mice, Inbred C57BL; D016202:N-Methylaspartate / Q000494:pharmacology; D017470:Receptors, Glutamate / Q000378:metabolism*; D012160:Retina / Q000166:cytology*; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
chemical_list: D018690:Excitatory Amino Acid Agonists; D017470:Receptors, Glutamate; D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; D008734:Methoxyhydroxyphenylglycol; D016202:N-Methylaspartate; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D002118:Calcium; D016257:Fura-2; C010117:3,4-dihydroxyphenylglycol