title: Association of TIP30 expression and prognosis of hepatocellular carcinoma in patients with HBV infection.
abstract: Altered expression of TIP30, a tumor suppressor, has been observed in many cancers. In this study, we have evaluated the expression of TIP30 in the tissues of 209 hepatocellular carcinomas (HCC) and their adjacent tissues by using a high-density tissue microarray, and analyzed its correlation with the clinical pathological parameters of the patients. The results revealed negative or weak expression of TIP30 in 43.5% (91/209) of the HCC tissues, and in only 27% (56/209) of the adjacent tissues. The expression level of TIP30 in HCC was inversely correlated with serum alpha-fetoprotein (AFP) levels, HBV infection, and tumor differentiation. Multivariate analysis for survival indicated that serum HBV infection was the most significant predictor of poor prognosis in HCC (P = 0.0023), and TIP30 expression and tumor differentiation were also independent indicators in this respect (P = 0.0364 and P = 0.0397, respectively). Patients with medium or high expression levels of TIP30 (TIP30(++/+++) ) had a better 5-year overall survival rate than those with low/negative (TIP30(+/-) ) expression (P < 0.001). TIP30(+/-/) HBV(+) patients had the worst 5-year overall survival rate, whereas TIP30(++/+++) /HBV(-) patients had the best. To further explore the correlation between TIP30 and HBV infection in HCC, HBV(+) hepatoblastoma cell-line HepG2 2.2.15 and HCC cell-line Hep3B were used. Upon silencing of HBV, we observed an upregulation of TIP30 and decreased cell proliferation. In the in vivo studies, we found that the mice inoculated with HepG2 2.2.15 cells with HBV silencing had a prolonged tumor latency and a longer life span, as compared to the control mice inoculated with untreated control cells. In conclusion, the results suggest that downregulation of TIP30 may result from HBV infection, and subsequently promotes the progression of HCC.
authors.affiliation: Department of Hepatology, Fuzhou General Hospital, Nanjing Command, Fuzhou 350025, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Hepatobiliary Surgery, Fuzhou General Hospital, Nanjing Command, Fuzhou 350025, China.
authors.forename: Lizhi
authors.initials: L
authors.lastname: Lv
authors.affiliation: Department of Oncology, Fuzhou General Hospital, Nanjing Command, Fuzhou 350025, China.
authors.forename: Xuenong
authors.initials: X
authors.lastname: Ouyang
authors.affiliation: Department of Hepatology, Fuzhou General Hospital, Nanjing Command, Fuzhou 350025, China.
authors.forename: Shi'an
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Hepatology, Fuzhou General Hospital, Nanjing Command, Fuzhou 350025, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Hepatology, Fuzhou General Hospital, Nanjing Command, Fuzhou 350025, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Cai
authors.affiliation: Department of Hepatology, Fuzhou General Hospital, Nanjing Command, Fuzhou 350025, China. lidliang9@163.com.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Li
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000328:Adult; D000368:Aged; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000209:etiology* / Q000401:mortality*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D006509:Hepatitis B / Q000150:complications* / Q000175:diagnosis; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics; D064593:Heterografts; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000175:diagnosis / Q000209:etiology* / Q000401:mortality*; D008297:Male; D051379:Mice; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012189:Retrospective Studies; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D006514:Hepatitis B Surface Antigens; D034741:RNA, Small Interfering; D014157:Transcription Factors; D000123:Acetyltransferases; C111246:HTATIP2 protein, human
keywords: HBV; Survival; TIP30; hepatocellular carcinomas; tumor suppressor