title: Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation.
abstract: BACKGROUND
  Calcium homeostasis is considered to be important in antineoplastic as well as in neurotoxic adverse effects of cisplatin.
  
  
  OBJECTIVE
  This study aimed to investigate the role of Ca(2+) in cisplatin neurotoxicity in cultured rat dorsal root ganglia (DRG) cells.
  
  
  METHODS
  Cell culture study.
  
  
  METHODS
  DRG cells prepared from 1-day old Sprague-Dawley rats were used to determine the role of Ca(2+) in the cisplatin (10-600 μM) neurotoxicity. The cells were incubated with cisplatin plus nimodipine (1-3 μM), dizocilpine (MK-801) (1-3 μM) or thapsigargin (100-300 nM). Toxicity of cisplatinon DRG cells was determined by the MTT assay.
  
  
  RESULTS
  The neurotoxicity of cisplatin was significant when used in high concentrations (100-600 μM). Nimodipine (1 μM) but not MK-801 or thapsigargin prevented the neurotoxic effects of 200 μM of cisplatin.
  
  
  CONCLUSIONS
  Voltage-dependent calcium channels may play a role in cisplatin neurotoxicity.
authors.affiliation: Department of Pharmacology, Osmangazi University School of Medicine, Eskişehir, Turkey.
authors.forename: Kevser
authors.initials: K
authors.lastname: Erol
authors.affiliation: Department of Pharmacology, Osmangazi University School of Medicine, Eskişehir, Turkey.
authors.forename: Semra
authors.initials: S
authors.lastname: Yiğitaslan
authors.affiliation: Department of Pharmacology, Osmangazi University School of Medicine, Eskişehir, Turkey.
authors.forename: Çiğdem
authors.initials: Ç
authors.lastname: Ünel
authors.affiliation: Department of Pharmacology, Osmangazi University School of Medicine, Eskişehir, Turkey.
authors.forename: Bilgin
authors.initials: B
authors.lastname: Kaygısız
authors.affiliation: Department of Pharmacology, Osmangazi University School of Medicine, Eskişehir, Turkey.
authors.forename: Engin
authors.initials: E
authors.lastname: Yıldırım
keywords: Calcium; cisplatin; dorsal root ganglia; primary cell culture