title: Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia.
abstract: Urine sampling for HPV DNA detection has been proposed as an effective method for monitoring the impact of HPV vaccination programs; however, conflicting results have been reported. The goal of this study was to evaluate the performance of optimized urine HPV DNA testing in women aged 19 to 25 years. Optimization process included the use of first void urine, immediate mixing of urine with DNA preservative, and the concentration of all HPV DNA, including cell-free DNA fragments. Urine and cervical samples were collected from 535 young women attending cervical screening at health centers from two Colombian cities. HPV DNA detection and genotyping was performed using an HPV type-specific multiplex genotyping assay, which combines multiplex polymerase chain reaction with bead-based Luminex technology. Concordance between HPV DNA detection in urine and cervical samples was determined using kappa statistics and McNemar tests. The accuracy of HPV DNA testing in urine samples was evaluated measuring sensitivity and specificity using as reference the results obtained from cervical samples. Statistical analysis was performed using STATA11.2 software. The findings revealed an overall HPV prevalence of 60.00% in cervical samples and 64.72% in urine samples, HPV-16 being the most frequent HPV type detected in both specimens. Moreover, our results indicate that detection of HPV DNA in first void urine provides similar results to those obtained with cervical samples and can be used to monitor HPV vaccination trials and programs as evidenced by the substantial concordance found for the detection of the four vaccine types. Cancer Prev Res; 9(9); 766-71. ©2016 AACR.
authors.affiliation: Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología (INC), Bogotá, Colombia. Departamento de Microbiología, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia. acombita@cancer.gov.co.
authors.forename: Alba Lucía
authors.initials: AL
authors.lastname: Cómbita
authors.affiliation: Infections and Cancer Biology Group, International Agency for Research on Cancer, Lyon, France.
authors.forename: Tarik
authors.initials: T
authors.lastname: Gheit
authors.affiliation: Proyecto Epidemiológico Guanacaste, Fundación INCIENSA, Guanacaste, Costa Rica.
authors.forename: Paula
authors.initials: P
authors.lastname: González
authors.affiliation: Grupo de Investigación en Salud Pública y Vigilancia Epidemiológica, Instituto Nacional de Cancerología (INC), Bogotá, Colombia.
authors.forename: Devi
authors.initials: D
authors.lastname: Puerto
authors.affiliation: Grupo de Investigación en Salud Pública y Vigilancia Epidemiológica, Instituto Nacional de Cancerología (INC), Bogotá, Colombia. Early Detection and Prevention Section, International Agency for Research on Cancer, Lyon, France.
authors.forename: Raúl Hernando
authors.initials: RH
authors.lastname: Murillo
authors.affiliation: Unidad de Análisis, Subdirección de Investigaciones, Instituto Nacional de Cancerología (INC), Bogotá, Colombia.
authors.forename: Luisa
authors.initials: L
authors.lastname: Montoya
authors.affiliation: Centre for the Evaluation of Vaccination, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerpen, Belgium.
authors.forename: Alex
authors.initials: A
authors.lastname: Vorsters
authors.affiliation: Centre for the Evaluation of Vaccination, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerpen, Belgium.
authors.forename: Severien
authors.initials: S
authors.lastname: Van Keer
authors.affiliation: Centre for the Evaluation of Vaccination, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerpen, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Van Damme
authors.affiliation: Infections and Cancer Biology Group, International Agency for Research on Cancer, Lyon, France.
authors.forename: Massimo
authors.initials: M
authors.lastname: Tommasino
authors.affiliation: Grupo de Investigación en Salud Pública y Vigilancia Epidemiológica, Instituto Nacional de Cancerología (INC), Bogotá, Colombia.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Hernández-Suárez
authors.affiliation: Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología (INC), Bogotá, Colombia.
authors.forename: Laura
authors.initials: L
authors.lastname: Sánchez
authors.affiliation: Early Detection and Prevention Section, International Agency for Research on Cancer, Lyon, France.
authors.forename: Rolando
authors.initials: R
authors.lastname: Herrero
authors.affiliation: Grupo de Investigación en Salud Pública y Vigilancia Epidemiológica, Instituto Nacional de Cancerología (INC), Bogotá, Colombia.
authors.forename: Carolina
authors.initials: C
authors.lastname: Wiesner
mesh_terms: D000328:Adult; D002584:Cervix Uteri / Q000821:virology*; D003105:Colombia; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D014556:Urine / Q000821:virology*; D014626:Vaginal Smears; D055815:Young Adult
chemical_list: D004279:DNA, Viral