title: Thermoresponsive mesoporous silica nanoparticles as a carrier for skin delivery of quercetin.
abstract: Recently, mesoporous silica nanoparticles (MSNs) have emerged as promising drug delivery systems able to preserve the integrity of the carried substance and/or to selectively reach a target site; however, they have rarely been explored for skin application. In this study, thermoresponsive MSNs, designed to work at physiologic cutaneous temperature, are proposed as innovative topical carriers for quercetin (Q), a well-known antioxidant. The thermosensitive nanoparticles were prepared by functionalizing two different types of matrices, with pore size of 3.5nm (MSNsmall) and 5.0nm (MSNbig), carrying out a free radical copolymerization of N-isopropylacrylamide (NIPAM) and 3-(methacryloxypropyl)trimethoxysilane (MPS) inside the mesopores. The obtained copolymer-grafted MSNs (copoly-MSNs) were physico-chemically characterized and their biocompatibility was attested on a human keratinocyte cell line (HaCaT). The release profiles were assessed and the functional activity of Q, free or loaded, was evaluated in terms of antiradical and metal chelating activities. Ex vivo accumulation and permeation through porcine skin were also investigated. The characterization confirmed the copolymer functionalization of the MSNs. In addition, both the bare and functionalized silica matrices were found to be biocompatible. Among the copolymer-grafted complexes, Q/copoly-MSNbig exhibited more evident thermoresponsive behavior proving the potential of these thermosensitive systems for advanced dermal delivery.
authors.affiliation: Università di Torino, Dipartimento di Scienza e Tecnologia del Farmaco, Via P. Giuria 9, 10125 Torino, Italy; NIS (Nanostructured Interfaces and Surfaces) Centre, Università di Torino, Italy; ⿿G. Scansetti⿿ Interdepartmental Centre, Università di Torino, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Ugazio
authors.affiliation: Università di Torino, Dipartimento di Scienza e Tecnologia del Farmaco, Via P. Giuria 9, 10125 Torino, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Gastaldi
authors.affiliation: Università di Torino, Dipartimento di Chimica, Via P. Giuria 7, 10125 Torino, Italy; NIS (Nanostructured Interfaces and Surfaces) Centre, Università di Torino, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Brunella
authors.affiliation: Università di Torino, Dipartimento di Chimica, Via P. Giuria 7, 10125 Torino, Italy; NIS (Nanostructured Interfaces and Surfaces) Centre, Università di Torino, Italy.
authors.forename: Dominique
authors.initials: D
authors.lastname: Scalarone
authors.affiliation: Università di Torino, Dipartimento di Chimica, Via P. Giuria 7, 10125 Torino, Italy; NIS (Nanostructured Interfaces and Surfaces) Centre, Università di Torino, Italy.
authors.forename: Sushilkumar A
authors.initials: SA
authors.lastname: Jadhav
authors.affiliation: Università di Torino, Dipartimento di Scienza e Tecnologia del Farmaco, Via P. Giuria 9, 10125 Torino, Italy; ⿿G. Scansetti⿿ Interdepartmental Centre, Università di Torino, Italy.
authors.forename: Simonetta
authors.initials: S
authors.lastname: Oliaro-Bosso
authors.affiliation: Università di Torino, Dipartimento di Scienza e Tecnologia del Farmaco, Via P. Giuria 9, 10125 Torino, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Zonari
authors.affiliation: Università di Torino, Dipartimento di Chimica, Via P. Giuria 7, 10125 Torino, Italy; NIS (Nanostructured Interfaces and Surfaces) Centre, Università di Torino, Italy.
authors.forename: Gloria
authors.initials: G
authors.lastname: Berlier
authors.affiliation: Università del Piemonte Orientale, Dipartimento di Scienze e Innovazione Tecnologica, Viale T. Michel 11, 15121, Alessandria, Italy.
authors.forename: Ivana
authors.initials: I
authors.lastname: Miletto
authors.affiliation: Università di Torino, Dipartimento di Scienza e Tecnologia del Farmaco, Via P. Giuria 9, 10125 Torino, Italy; NIS (Nanostructured Interfaces and Surfaces) Centre, Università di Torino, Italy; ⿿G. Scansetti⿿ Interdepartmental Centre, Università di Torino, Italy. Electronic address: simona.sapino@unito.it.
authors.forename: Simona
authors.initials: S
authors.lastname: Sapino
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism; D002461:Cell Line, Transformed; D004337:Drug Carriers / Q000008:administration & dosage* / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage* / Q000378:metabolism; D009924:Organ Culture Techniques; D016062:Porosity; D011794:Quercetin / Q000008:administration & dosage* / Q000378:metabolism; D012822:Silicon Dioxide / Q000008:administration & dosage*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D013552:Swine
chemical_list: D000975:Antioxidants; D004337:Drug Carriers; D012822:Silicon Dioxide; D011794:Quercetin
keywords: Antioxidant; Biocompatibility; Controlled release; Copolymer; Cutaneous application; MCM-41; NIPAM