title: Regulator of Calcineurin 1 in Periodontal Disease.
abstract: Nuclear factor of activated T-cells (NFAT) and NF-kB pathway associated processes are involved in the pathogenesis of various inflammatory disorders, for example, periodontal disease. The activation of these pathways is controlled by the regulator of calcineurin 1 (RCAN1). The aim of this study was to elucidate the role of RCAN1 in periodontal disease. Healthy and inflamed periodontal tissues were analyzed by immunohistochemistry and immunofluorescence using specific rabbit polyclonal anti-RCAN1 antibodies. For expression analysis human umbilical vein endothelial cells (HUVEC) were used. HUVEC were incubated for 2 h with Vascular Endothelial Growth Factor (VEGF) or with wild type and laboratory strains of Porphyromonas gingivalis (P. gingivalis). Expression analysis of rcan1 and cox2 was done by real time PCR using specific primers for rcan1.4 and cox2. The expression of rcan1 was found to be significantly suppressed in endothelial cells of chronically inflamed periodontal tissues compared to healthy controls. Rcan1 and cox2 were significantly induced by VEGF and wild type and laboratory P. gingivalis strains. Interestingly, the magnitude of the rcan1 and cox2 induction was strain dependent. The results of this study indicate that RCAN1 is suppressed in endothelial cells of chronically inflamed periodontal tissues. During an acute infection, however, rcan1 seems to be upregulated in endothelial cells, indicating a modulating role in immune homeostasis of periodontal tissues.
authors.affiliation: Section of Periodontics, Heinrich-Heine University, 40225 Düsseldorf, Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Peters
authors.affiliation: Section of Periodontics, Heinrich-Heine University, 40225 Düsseldorf, Germany.
authors.forename: Eleni
authors.initials: E
authors.lastname: Solominidou
authors.affiliation: Institute for Experimental Dental Research and Oral Musculoskeletal Biology, Center for Biochemistry, University of Cologne, 50931 Cologne, Germany.
authors.forename: Yüksel
authors.initials: Y
authors.lastname: Korkmaz
authors.affiliation: Department of Operative Dentistry, Center for Dentistry and Oral Medicine (Carolinum), Goethe-University Frankfurt, 60596 Frankfurt, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Rüttermann
authors.affiliation: Section of Periodontics, Heinrich-Heine University, 40225 Düsseldorf, Germany.
authors.forename: Astrid
authors.initials: A
authors.lastname: Klocke
authors.affiliation: Faculty of Dentistry, The University of Hong Kong, Sai Ying Pun, Hong Kong.
authors.forename: Thomas Frank
authors.initials: TF
authors.lastname: Flemmig
authors.affiliation: Section of Periodontics, Heinrich-Heine University, 40225 Düsseldorf, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Beikler
mesh_terms: D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D010510:Periodontal Diseases / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology*; D010519:Periodontium / Q000378:metabolism* / Q000473:pathology*; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
chemical_list: D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009124:Muscle Proteins; C108612:RCAN1 protein, human; D042461:Vascular Endothelial Growth Factor A; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human