title: Baltikinin: A New Myotropic Tryptophyllin-3 Peptide Isolated from the Skin Secretion of the Purple-Sided Leaf Frog, Phyllomedusa baltea.
abstract: Here we report the identification of a novel tryptophyllin-3 peptide with arterial smooth muscle relaxation activity from the skin secretion of the purple-sided leaf frog, Phyllomedusa baltea. This new peptide was named baltikinin and had the following primary structure, pGluDKPFGPPPIYPV, as determined by tandem mass spectrometry (MS/MS) fragmentation sequencing and from cloned skin precursor-encoding cDNA. A synthetic replicate of baltikinin was found to have a similar potency to bradykinin in relaxing arterial smooth muscle (half maximal effective concentration (EC50) is 7.2 nM). These data illustrate how amphibian skin secretions can continue to provide novel potent peptides that act through functional targets in mammalian tissues.
authors.affiliation: Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. dshi01@qub.ac.uk.
authors.forename: Daning
authors.initials: D
authors.lastname: Shi
authors.affiliation: Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. x.xi@qub.ac.uk.
authors.forename: Xinping
authors.initials: X
authors.lastname: Xi
authors.affiliation: Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. l.wang@qub.ac.uk.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. ygao07@qub.ac.uk.
authors.forename: Yitian
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. c.ma@qub.ac.uk.
authors.forename: Chengbang
authors.initials: C
authors.lastname: Ma
authors.affiliation: Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. chenhang2012@163.com.|Fujian Provincial Institute for Food and Drug Quality Control, Fuzhou, Fujian 350000, China. chenhang2012@163.com.
authors.forename: Hang
authors.initials: H
authors.lastname: Chen
authors.affiliation: Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. m.zhou@qub.ac.uk.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. t.chen@qub.ac.uk.
authors.forename: Tianbao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. chris.shaw@qub.ac.uk.
authors.forename: Chris
authors.initials: C
authors.lastname: Shaw
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D003001:Cloning, Molecular; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D011898:Ranidae / Q000235:genetics / Q000378:metabolism*; D017208:Rats, Wistar; D012867:Skin / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014664:Vasodilation / Q000187:drug effects*; D014665:Vasodilator Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D029845:Amphibian Proteins; D009842:Oligopeptides; D014665:Vasodilator Agents; C000624888:baltikinin peptide, Phyllomedusa baltea; C000595094:tryptophyllin type 3 peptide, PhT-3
keywords: amphibian; molecular cloning; peptide; smooth muscle