title: Macroscopic and Fluorescent Discrimination of Adenosine Triphosphate via Selective Metallo-hydrogel Formation: A Visual, Practical, and Reliable Rehearsal toward Cellular Imaging.
abstract: With use of simple terpyridine zinc nitrate complexes, intriguing visual recognition of adenosine triphosphate (ATP) via selective coordination assembly leading to two-component metallo-hydrogel formation has been realized. With intensive fluorescent study and density functional theory calculations, it may be inferred, besides the selective metal-ligand interaction between Zn center and phosphate groups, the intramolecular π-stacking between the planar nucleobases of ATP and the metal-hybrid aromatic ring of pincer complex strongly affected the geometry of the coordinated adducts and possible molecular self-assembly process, which constitute a completely new sensing strategy in comparison with the conventional approaches. Furthermore, in light of extreme sensitivity of pincer zinc complexes toward ATP at micromolar scale (1.85 μM) and remarkable fluorescent enhancement (ca. 44-fold) upon ATP addition, the feasibility of the low cytotoxicity pincer zinc complexes in monitoring ATP in HeLa cells has been fulfilled with confocal fluorescence microscopy.
authors.affiliation: Department of Chemistry, Fudan University , 220 Handan Road, Shanghai 200433, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Fang
authors.affiliation: Department of Chemistry, Fudan University , 220 Handan Road, Shanghai 200433, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, The Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences , Yantai 264003, China.
authors.forename: Fabiao
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Chemistry, Fudan University , 220 Handan Road, Shanghai 200433, China.
authors.forename: Yaoqi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Fudan University , 220 Handan Road, Shanghai 200433, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, The Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences , Yantai 264003, China.
authors.forename: Lingxin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Quality Inspection of Food and Cosmetics, Shanghai Institute of Quality Inspection and Technical Research , 381 Cangwu Road, Shanghai 200233, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Bao
authors.affiliation: Department of Chemistry, Fudan University , 220 Handan Road, Shanghai 200433, China.|State Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences , Shanghai 200032, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tu
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D020100:Hydrogels; D013050:Spectrometry, Fluorescence; D015032:Zinc
chemical_list: D020100:Hydrogels; D000255:Adenosine Triphosphate; D015032:Zinc
keywords: adenosine triphosphate; celluar imaging; fluorescence enhancement; metallo-hydrogel; visual recognition