title: Differing features of proteins in membranes may result in antioxidant or prooxidant action: opposite effects on lipid peroxidation of alcohol dehydrogenase and albumin in liposomal systems.
abstract: The influence of 3 thiol-containing compounds, bovine serum albumin (fatty acid free: BSA), glutathione (GSH) and yeast alcohol dehydrogenase (YADH) on lipid peroxidation in multilamellar liposomes, prepared from ox-brain phospholipid, was investigated. Thiol-compounds were added either before liposome formation, or after liposome formation; and their effects compared to a positive control. Bovine serum albumin (BSA), an acidic hydrophilic protein, displays a small, concentration dependent, antioxidant effect when added to preformed liposomes. A much larger antioxidant effect was observed when the BSA was entrapped inside the liposome, by adding BSA just prior to liposome preparation. In contrast, a Zn(2+) containing redox enzyme, YADH, a basic hydrophobic membrane-associating protein, displays a large pro-oxidant effect at much lower concentrations especially when entrapped inside the liposome. This was observed also with GSH; but per mole of -SH, YADH was about 18 times as powerful a pro-oxidant perhaps because of structural changes to the membrane. Oxidized glutathione and N-acetylcysteine were also pro-oxidant (cysteine and cystine showed little effect). Formation of thiyl radicals may occur in the presence of iron ions with these pro-oxidant sulphur-containing compounds. Partial protection against lipid peroxidation was observed with EDTA, desferrioxamine and protoporphyrin (IX), potent iron-chelating agents.
authors.affiliation: a Molecular Toxicology Group , School of Biological Sciences, University of Surrey , Guildford , UK.
authors.forename: A
authors.initials: A
authors.lastname: Riedl
authors.affiliation: a Molecular Toxicology Group , School of Biological Sciences, University of Surrey , Guildford , UK.
authors.forename: Z
authors.initials: Z
authors.lastname: Shamsi
authors.affiliation: a Molecular Toxicology Group , School of Biological Sciences, University of Surrey , Guildford , UK.
authors.forename: M
authors.initials: M
authors.lastname: Anderton
authors.affiliation: a Molecular Toxicology Group , School of Biological Sciences, University of Surrey , Guildford , UK.
authors.forename: P
authors.initials: P
authors.lastname: Goldfarb
authors.affiliation: a Molecular Toxicology Group , School of Biological Sciences, University of Surrey , Guildford , UK.
authors.forename: A
authors.initials: A
authors.lastname: Wiseman