title: Application of APTES-Anti-E-cadherin film for early cancer monitoring.
abstract: Cancer staging is a way to classify cancer according to the extent of the disease in the body. The stage is usually determined by several factors such as the location of the primary tumor, the tumor size, the degree of spread in the surrounding tissues, etc. The study of E-cadherin (EC) expression on cancerous cells of patients has revealed variations in the molecular expression patterns of primary tumors and metastatic tumors. The detection of these cells requires a long procedure involving conventional techniques, thus, the requirement for development of new rapid devices that permit direct and highly sensitive detection stimulates the sensing field progress. Here, we explore if E-cadherin could be used as a biomarker to bind and detect epithelial cancer cells. Hence, the sensitive and specific detection of E-cadherin expressed on epithelial cells is approached by immobilizing anti-E-cadherin antibody (AEC) onto aminosilanized indium-tin oxide (ITO) surface. The immunosensing surfaces have been characterized by electrochemical measurements, wettability and confocal microscopy and their performance has been assessed in the presence of cancer cell lines. Under optimal conditions, the resulting immunosensor displayed a selective detection of E-cadherin expressing cells, which could be detected either by fluorescence or electrochemical techniques. The developed immunosensing surface could provide a simple tool that can be applied to cancer staging.
authors.affiliation: Laboratory of biophysics, Faculty of Medecine of Monastir, University of Monastir, Tunisia. Electronic address: manel.benismail@gmail.com.
authors.forename: Manel
authors.initials: M
authors.lastname: Ben Ismail
authors.affiliation: Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules, ERRMECe (EA 1391), Institut des Matériaux, University of Cergy-Pontoise, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Carreiras
authors.affiliation: Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules, ERRMECe (EA 1391), Institut des Matériaux, University of Cergy-Pontoise, France.
authors.forename: Rémy
authors.initials: R
authors.lastname: Agniel
authors.affiliation: Laboratory of biochemistry, Faculty of Medecine of Monastir, University of Monastir, Tunisia.
authors.forename: Donia
authors.initials: D
authors.lastname: Mili
authors.affiliation: Laboratory of biophysics, Faculty of Medecine of Monastir, University of Monastir, Tunisia; Groupe Immunité des Muqueuses et des Agents Pathogènes (GIMAP), Faculty of Medecine Jacques Lisfranc of Saint-Etienne, France.
authors.forename: Dejla
authors.initials: D
authors.lastname: Sboui
authors.affiliation: Laboratory of biophysics, Faculty of Medecine of Monastir, University of Monastir, Tunisia.
authors.forename: Nahla
authors.initials: N
authors.lastname: Zanina
authors.affiliation: Laboratory of biophysics, Faculty of Medecine of Monastir, University of Monastir, Tunisia.
authors.forename: Ali
authors.initials: A
authors.lastname: Othmane
mesh_terms: D000906:Antibodies / Q000032:analysis; D015415:Biomarkers / Q000032:analysis* / Q000737:chemistry; D015820:Cadherins / Q000032:analysis* / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D018613:Microscopy, Confocal; D017971:Tin Compounds / Q000737:chemistry; D017155:Wettability
chemical_list: D000906:Antibodies; D015415:Biomarkers; D015820:Cadherins; D017971:Tin Compounds; C109984:indium tin oxide
keywords: Cancer; E-cadherin; Epithelial cells; Immunosensing surface