title: In vivo two-photon imaging of striatal neuronal circuits in mice.
abstract: Imaging studies of the subcortical striatum in vivo have been technically challenging despite its functional importance in movement control and procedural learning. Here, we report a method for imaging striatal neuronal circuits in mice in vivo using two-photon microscopy. Cell bodies and intermingled dendrites of GABAergic neurons labeled with fluorescent proteins were imaged in the dorsal striatum through an imaging window implanted in the overlying cortex. This technique could be highly useful for studying the structure and function of striatal networks at cellular and subcellular resolutions in normal mice, as well as in mouse models of neurological disorders.
authors.affiliation: RIKEN Brain Science Institute, Wako, Saitama, Japan; PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan. Electronic address: msato@brain.riken.jp.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Sato
authors.affiliation: RIKEN Brain Science Institute, Wako, Saitama, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Kawano
authors.affiliation: Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Yuchio
authors.initials: Y
authors.lastname: Yanagawa
authors.affiliation: RIKEN Brain Science Institute, Wako, Saitama, Japan; Brain Science Institute, Saitama University, Saitama, Japan; School of Life Science, South China Normal University, Guangzhou, China; Department of Pharmacology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000000981:diagnostic imaging*; D049229:Dendritic Spines; D000229:Dependovirus; D018274:Electroporation; D059330:GABAergic Neurons; D051379:Mice; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009415:Nerve Net / Q000000981:diagnostic imaging*
keywords: Adeno-associated virus; Basal ganglia; Deep brain imaging; In utero electroporation; Striatum; Two-photon microscopy