title: Embedding carotenoids of spheroidene-branch biosynthesis into antenna complexes of sulfur photosynthetic bacteria.
abstract: The possibility of embedding the carotenoids of spheroidene-branch biosynthesis (spheroidene and spheroidenone) from non-sulfur bacteria into the diphenylamine antenna complexes (DPA-complexes) from the sulfur bacteria Allochromatium minutissimum and Ectothiorhodospira haloalkaliphila with carotenoid synthesis inhibited by diphenylamine (DPA) was studied for the first time. It was found that spheroidene was embedded into the DPA-complexes from these bacteria at a level of 75-87%, with spheroidene embedding efficiency being 41-68% for the LH1-RC DPA-complexes and 71-89% for the LH2 DPA-complexes. The energy transfer efficiency from carotenoids to bacteriochlorophyll was shown to depend not only on the type of carotenoid but also on the very structure on the antenna complex.
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, ul. Institutskaya 2, Pushchino, Moscow oblast, 142290, Russia. AshikhminAA@gmail.com.
authors.forename: A A
authors.initials: AA
authors.lastname: Ashikhmin
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, ul. Institutskaya 2, Pushchino, Moscow oblast, 142290, Russia.
authors.forename: Z K
authors.initials: ZK
authors.lastname: Makhneva
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, ul. Institutskaya 2, Pushchino, Moscow oblast, 142290, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Bolshakov
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, ul. Institutskaya 2, Pushchino, Moscow oblast, 142290, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Shastik
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, ul. Institutskaya 2, Pushchino, Moscow oblast, 142290, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Moskalenko
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry*; D002338:Carotenoids / Q000096:biosynthesis / Q000737:chemistry*; D002841:Chromatiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004159:Diphenylamine / Q000494:pharmacology; D020587:Ectothiorhodospira / Q000737:chemistry*; D004586:Electrophoresis; D045342:Light-Harvesting Protein Complexes / Q000096:biosynthesis / Q000737:chemistry*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013057:Spectrum Analysis
chemical_list: D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D011500:Protein Synthesis Inhibitors; C005637:spheroidene; C104880:spheroidenone; D002338:Carotenoids; D004159:Diphenylamine