title: The efficacy of Aesculus hippocastanum seeds on diabetic nephropathy in a streptozotocin-induced diabetic rat model.
abstract: Cytokines, such as transforming growth factor (TGF)-ß1, and increased oxidative stress are considered to be responsible for the development of diabetic nephropathy. We hypothesized that Aesculus hippocastanum (AH) seeds may have preventive effects on oxidative stress and TGF-β-related diabetic nephropathy in streptozotocin (STZ)-induced diabetic nephropathy in rats. Twenty-one male Sprague-Dawley albino rats were divided into three groups (n=7). Except for the control group, they all had diabetic nephropathy induced by an intraperitoneal injection of STZ. While the diabetes group did not receive any medication, the diabetes+AH group was given the medication for 4 weeks. After the experiment, analyses were performed to evaluate the glomerular area, severity of sclerosis, and fibronectin immunoexpression, as well as levels of malondialdehyde (MDA), TGF-β, blood urea nitrogen (BUN), blood glucose, creatinine, and proteinuria. It was found that glomerular area, severity of sclerosis, fibronectin immunoexpression, and levels of MDA, TGF-β, BUN, creatinine, and proteinuria were decreased in the diabetes+AH group. It is known that diabetic nephropathy is induced, to a large extent, by hyperglycemia. In the present study, AH extract ameliorated diabetic nephropathy without decrease in blood glucose levels. In the study, AH seeds showed beneficial effects on the functional properties of the kidney and microscopic improvements in diabetic nephropathy.
authors.affiliation: Department of Physiology, Faculty of Medicine, Mugla Sitki Kocman University, 48000, Mugla, Turkey. Electronic address: onurelmas@outlook.com.
authors.forename: Onur
authors.initials: O
authors.lastname: Elmas
authors.affiliation: Department of Physiology, Faculty of Medicine, Bilim University, 34000, Istanbul, Turkey.
authors.forename: Oytun
authors.initials: O
authors.lastname: Erbas
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Ege University, 35000, Izmir, Turkey.
authors.forename: Gurkan
authors.initials: G
authors.lastname: Yigitturk
mesh_terms: D031319:Aesculus / Q000737:chemistry*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D003928:Diabetic Nephropathies / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D007678:Kidney Glomerulus / Q000187:drug effects / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry*; D013311:Streptozocin; D016212:Transforming Growth Factor beta; D016896:Treatment Outcome
chemical_list: D001786:Blood Glucose; D010936:Plant Extracts; D016212:Transforming Growth Factor beta; D008315:Malondialdehyde; D013311:Streptozocin; D003404:Creatinine
keywords: Aesculus hippocastanum; Diabetic nephropathy; Oxidative stress; Rats; Streptozotocin induced diabetes