title: Interaction of three-finger proteins from snake venoms and from mammalian brain with the cys-loop receptors and their models.
abstract: With the use of surface plasmon resonance (SPR) it was shown that ws-Lynx1, a water-soluble analog of the three-finger membrane-bound protein Lynx1, that modulates the activity of brain nicotinic acetylcholine receptors (nAChRs), interacts with the acetylcholine-binding protein (AChBP) with high affinity, K D = 62 nM. This result agrees with the earlier demonstrated competition of ws-Lynx1 with radioiodinated α-bungarotoxin for binding to AChBP. For the first time it was shown that ws-Lynx1 binds to GLIC, prokaryotic Cys-loop receptor (K D = 1.3 μM). On the contrary, SPR revealed that α-cobratoxin, a three-finger protein from cobra venom, does not bind to GLIC. Obtained results indicate that SPR is a promising method for analysis of topography of ws-Lynx1 binding sites using its mutants and those of AChBP and GLIC.
authors.affiliation: Pasteur Institute, Paris, France. ner-neri@yandex.ru.
authors.forename: G
authors.initials: G
authors.lastname: Faure
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Shelukhina
authors.affiliation: Pasteur Institute, Paris, France.|Biochemistry Department, Nicolaus Copernicus University, Torun, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Porowinska
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Shulepko
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Lyukmanova
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Dolgikh
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Spirova
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia.
authors.forename: I E
authors.initials: IE
authors.lastname: Kasheverov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia.
authors.forename: Yu N
authors.initials: YN
authors.lastname: Utkin
authors.affiliation: Pasteur Institute, Paris, France.
authors.forename: J-P
authors.initials: JP
authors.lastname: Corringer
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Tsetlin
mesh_terms: D000818:Animals; D001048:Aplysia; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D003039:Cobra Neurotoxin Proteins / Q000378:metabolism*; D000458:Cyanobacteria; D058466:Cysteine Loop Ligand-Gated Ion Channel Receptors / Q000737:chemistry / Q000378:metabolism*; D004331:Drosophila melanogaster; D004546:Elapid Venoms / Q000737:chemistry / Q000378:metabolism; D017815:Elapidae; D004926:Escherichia coli; D057809:HEK293 Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D020349:Surface Plasmon Resonance; D064569:alpha7 Nicotinic Acetylcholine Receptor / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003039:Cobra Neurotoxin Proteins; D058466:Cysteine Loop Ligand-Gated Ion Channel Receptors; D004546:Elapid Venoms; D008562:Membrane Glycoproteins; C521753:Naja kaouthia venom; D064569:alpha7 Nicotinic Acetylcholine Receptor; C025154:alpha-cobratoxin