title: [Prevalence of Helicobacter pylori cagA, vacA, and iceA genotypes in children with gastroduodenal diseases].
abstract: To investigate the prevalence of cagA, vacA, and iceA genotypes in the isolated strains of Helicobacter pylori (H.pylori) from children with gastroduodenal diseases in Jiangxi, China, as well as the association between cagA, vacA, and iceA genotypes and the type of gastroduodenal diseases.
  
  
  
  The samples of gastric antral mucosa were collected from 316 children with gastroduodenal diseases in Jiangxi, and a total of 107 strains of H.pylori were isolated. The genomic DNA of these strains was extracted, and PCR was used to determine the ureA, cagA, vacA, and iceA genotypes.
  
  
  
  Of all the 107 isolated strains of H.pylori, the detection rates of ureA and cagA genes were 100% (107/107) and 94.4% (101/107) respectively. The overall detection rate of vacA gene was 100% (107/107), and the detection rates of vacAs1a, vacAs1c, vacAm1, and vacAm2 genes were 74.8% (80/107), 25.2% (27/107), 29.9% (32/107), and 69.2% (74/107) respectively, with both vacAm1 and vacAm2 genes detected in 0.9% (1/107) of all H.pylori strains. In the chimera of vacA gene, the detection rates of vacAs1a/m1, vacAs1a/m2, vacAs1c/m1, and vacAs1c/m2 genes were 26.2% (28/107), 51.4% (55/107), 3.7% (4/107), and 17.8% (19/107) respectively (P<0.001). The detection rates of iceA1 and iceA2 genes were 79.4% (85/107) and 9.3% (10/107), respectively (P<0.001), and both iceA1 and iceA2 genes were detected in 7.5% (8/107) of all strains. The detection rates of the genotypes of H.pylori showed no significant differences between the peptic ulcer, chronic gastritis, and duodenal bulbar inflammation groups (P>0.05).
  
  
  
  The dominant genotypes of H.pylori are cagA, vacAs1a/m2, and iceA1, and there are mixed infections with H.pylori strains of different genotypes in children with gastroduodenal disease from Jiangxi, China. The genotypes of H.pylori are not associated with the type of gastroduodenal disease.
authors.affiliation: Department of Digestive Medicine, First Affiliated Hospital of Nanchang University, Nanchang 330006, China. jyyfyzx@163.com.
authors.forename: Shuang-Hong
authors.initials: SH
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Xie
authors.forename: Bi-Min
authors.initials: BM
authors.lastname: Li
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Liu
authors.forename: Sheng-Hua
authors.initials: SH
authors.lastname: Wan
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Luo
authors.forename: Zhen-Jun
authors.initials: ZJ
authors.lastname: Xiao
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Yi
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000293:Adolescent; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005756:Gastritis / Q000382:microbiology*; D005838:Genotype; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D008297:Male; D010437:Peptic Ulcer / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; C063113:ice nucleation protein