title: Molecular Imaging of CD44-Overexpressing Gastric Cancer in Mice Using T2 MR Imaging.
abstract: Novel diagnostic techniques have been developed in many research area using targetable contrast agents with magnetic resonance imaging (MRI) for cancer diagnosis. For cancer diagnosis, the use of MRI with biocompatible targeting moieties and manganese ferrite nanoparticles (MFNPs) is preferred. Thus, we synthesized MFNPs using a thermal decomposition method which enables sensitive T2 or T2 Turbo Spin Echo (TSE) MRI and coated them with hyaluronic acid (HA). The high targeting ability of HA-MFNPs was observed at MKN-45 cells (gastric cancer cell line) which high-expressing CD44 in contrast with MKN-28 cells which low-expressing CD44. We also prepared the gastric cancer mice model using MKN-45 cells which has the stem-like property was implanted into BALB/c nude mice. And then HA-MFNPs of the T2 contrast enhancement effects and targeting ability were investigated by in vivo MR imaging. As a result of these studies, we conclude that HA coated MFNPs can be effectively used as a novel probes for visualizing gastric cancer stem cells.
authors.forename: Hwunjae
authors.initials: H
authors.lastname: Lee
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Yang
authors.forename: Dan
authors.initials: D
authors.lastname: Heo
authors.forename: Heyoung
authors.initials: H
authors.lastname: Son
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
authors.forename: Jin-Suck
authors.initials: JS
authors.lastname: Suh
authors.forename: Jaemoon
authors.initials: J
authors.lastname: Yang
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology; D018960:Hyaluronan Receptors / Q000096:biosynthesis*; D008279:Magnetic Resonance Imaging; D017895:Manganese Compounds / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D057054:Molecular Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging / Q000378:metabolism; D011859:Radiography; D013274:Stomach Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism
chemical_list: C476399:Cd44 protein, mouse; D003287:Contrast Media; D005290:Ferric Compounds; D018960:Hyaluronan Receptors; D017895:Manganese Compounds; D009363:Neoplasm Proteins; C551151:manganese ferrite