title: Parvovirus B19 Passive Transmission by Transfusion of Intercept® Blood System-Treated Platelet Concentrate.
abstract: BACKGROUND
  Pathogen reduction methods for blood components are effective for a large number of viruses though less against small, non-enveloped viruses such as Parvovirus B19 (B19V). This article describes the passive transmission by transfusion of two B19V-contaminated pooled platelet concentrates (PCs) which were treated with the Intercept® blood pathogen reduction system.
  
  
  METHODS
  Two transfusion cases of B19V-contaminated Intercept-treated pooled PCs were described. Due to the analysis delay, the PCs were already transfused. The viral content of each donation was 4.87 × 10(10) IU/ml in case 1and 1.46 × 10(8) IU/ml in case 2. B19V (52 IU/ml) was detected in the recipient of the case 1 PC, whereas no virus could be detected in the case 2 PC recipient. A B19V IgM response and a transient boost of the underlying B19V IgG immune status and was observed in recipient 1. Recipient of the case 2 PC remained B19V IgG- and IgM-negative. B19V DNA sequence and phylogenetic analysis revealed a 100% homology between donor and recipient.
  
  
  CONCLUSIONS
  This report describes passive B19V transmission by a PC with very high B19 viral load which elicited a transient boost of the B19V immunity, but not by a PC with a lower B19V content, suggesting that there is a B19 viral load threshold value at which B19V inactivation is exceeded.
authors.affiliation: Interregional Blood Transfusion SRC, Bern, Switzerland.
authors.forename: Peter
authors.initials: P
authors.lastname: Gowland
authors.affiliation: Interregional Blood Transfusion SRC, Bern, Switzerland.
authors.forename: Stefano
authors.initials: S
authors.lastname: Fontana
authors.affiliation: Interregional Blood Transfusion SRC, Bern, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Stolz
authors.affiliation: University Clinic of Haematology and Central Haematology Laboratory, Bern University Hospital Inselspital, University of Bern, Bern, Switzerland.
authors.forename: Nicola
authors.initials: N
authors.lastname: Andina
authors.affiliation: Interregional Blood Transfusion SRC, Bern, Switzerland.
authors.forename: Christoph
authors.initials: C
authors.lastname: Niederhauser
keywords: Parvovirus B19; Pathogen inactivation; Pathogen reduction; Platelet transfusion; Transfusion-associated infections