title: Core-shell self-assembly triggered via a thiol-disulfide exchange reaction for reduced glutathione detection and single cells monitoring.
abstract: A novel core-shell DNA self-assembly catalyzed by thiol-disulfide exchange reactions was proposed, which could realize GSH-initiated hybridization chain reaction (HCR) for signal amplification and molecules gathering. Significantly, these self-assembled products via electrostatic interaction could accumulate into prominent and clustered fluorescence-bright spots in single cancer cells for reduced glutathione monitoring, which will effectively drive cell monitoring into a new era.
authors.affiliation: Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan 250014, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan 250014, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000476:Alkanesulfonates / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry; D002460:Cell Line; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry* / Q000378:metabolism; D019793:Fluorescein / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D005978:Glutathione / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020014:K562 Cells; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D059010:Single-Cell Analysis / Q000379:methods*; D055672:Static Electricity
chemical_list: D000476:Alkanesulfonates; D001391:Azo Compounds; C000598590:BHQ-1 compound; D004220:Disulfides; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D004247:DNA; D005978:Glutathione; D019793:Fluorescein