title: Fabrication of FGF-2 immobilized electrospun gelatin nanofibers for tissue engineering.
abstract: Conjugated gelatin nanofibers were fabricated by electrospinning, followed by a simple glutaraldehyde cross-linking procedure and avidin conjugation. Then, biotinylated growth factors were immobilized onto the surface of the fibers through avidin-biotin covalent binding. The immobilization of growth factors was confirmed through immunostaining using fluorescence microscopy and microplate spectrophotometry. Adipose derived stem cells (ASCs) were cultured to examine the effect of immobilized growth factors on cell proliferation using the cell counting Kit-8 (CCK-8) assay. Gelatin nanofibers with no growth factors attached and growth factors in suspension within media were used as controls. Growth factors were successfully immobilized onto the surface, in amounts corresponding to the concentrations applied, and increased cell proliferation to a higher extend than growth factors in suspension. Our results suggest that this controllable scaffolding strategy provides an effective system for growth factors delivery in tissues, suitable for engineering applications.
authors.affiliation: School of Integrative Engineering, Chung-Ang University, Republic of Korea.
authors.forename: Haram
authors.initials: H
authors.lastname: Lee
authors.affiliation: School of Integrative Engineering, Chung-Ang University, Republic of Korea.
authors.forename: Sooseok
authors.initials: S
authors.lastname: Lim
authors.affiliation: School of Integrative Engineering, Chung-Ang University, Republic of Korea.
authors.forename: Mallinath S
authors.initials: MS
authors.lastname: Birajdar
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Republic of Korea. Electronic address: soohong@cha.ac.kr.
authors.forename: Soo-Hong
authors.initials: SH
authors.lastname: Lee
authors.affiliation: School of Integrative Engineering, Chung-Ang University, Republic of Korea. Electronic address: heyshoo@cau.ac.kr.
authors.forename: Hansoo
authors.initials: H
authors.lastname: Park
mesh_terms: D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D055664:Electrochemical Techniques; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry* / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry* / Q000494:pharmacology; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D055767:Immobilized Proteins; D016222:Fibroblast Growth Factor 2; D005780:Gelatin
keywords: Avidin-biotin; Cell proliferation etc.; Cross-linking; Gelatin nanofibers; Growth factor