title: The Effect of Hemodialysis on the Expression of Platelet Glycoproteins Ib, IIb/IIIa and P-Selectin.
abstract: To determine the effect of hemodialysis on expression of platelet receptors in patients with chronic renal failure.
  
  
  METHODS
  Blood sampling performed in chronic HD patients prior to the dialysis session, then 15 and 180 minutes into HD. Both dialysis machine inlet and outlet samples were taken at 15 minutes. Control subjects had a single blood sample taken.
  
  
  METHODS
  Thirteen adult males on chronic hemodialysis and 20 age and sex matched healthy controls.
  
  
  METHODS
  Flow-cytometric analysis of platelet GP-Ib, GP-IIb/IIIa, and P-selectin. Plasma vWF multimers were analyzed by SDS-polyacrylamide gel electrophoresis and immunoblotting.
  
  
  RESULTS
  Mean channel fluorescence (MCF) for GP lb was significantly (P < 0.05) decreased in pre-dialysis patients compared to controls and decreased further 3 hours into the dialysis session compared to the start of the dialysis treatment (P < 0.01). MCF for GP IIb/IIIa between predialysis patients and controls was similar, but decreased after a single dialysis session (P < 0.01). MCF for P-selectin on platelets was similar in patients and controls, but fewer platelets from the patients expressed P-selectin compared to controls (P < 0.05). Qualitative multimeric analysis of the vWF in patients, pre- and post-HD was unchanged.
  
  
  CONCLUSIONS
  Alterations in platelet surface expression of GPIb, GPIIb/IIIa, and P-selectin may, partially contribute to the changes in platelet function seen in patients on hemodialysis. It is unlikely that alterations in the surface expression of these receptors alone can adequately account for the complex platelet and hemostatic changes associated with uremia and the HD procedure.
authors.affiliation: a Department of Pathology , and Hypertension, Department of Internal Medicine , James A. Haley Veterans Hospital and the University of South Florida, College of Medicine , Tampa , Florida.
authors.forename: M B
authors.initials: MB
authors.lastname: Morgan
authors.affiliation: a Department of Pathology , and Hypertension, Department of Internal Medicine , James A. Haley Veterans Hospital and the University of South Florida, College of Medicine , Tampa , Florida.
authors.forename: S J
authors.initials: SJ
authors.lastname: Agosti
authors.affiliation: a Department of Pathology , and Hypertension, Department of Internal Medicine , James A. Haley Veterans Hospital and the University of South Florida, College of Medicine , Tampa , Florida.
authors.forename: P
authors.initials: P
authors.lastname: Foulis
authors.affiliation: b Division of Nephrology and Hypertension, Department of Internal Medicine , James A. Haley Veterans Hospital and the University of South Florida, College of Medicine , Tampa , Florida.
authors.forename: G
authors.initials: G
authors.lastname: Ramirez
authors.affiliation: b Division of Nephrology and Hypertension, Department of Internal Medicine , James A. Haley Veterans Hospital and the University of South Florida, College of Medicine , Tampa , Florida.
authors.forename: H
authors.initials: H
authors.lastname: Rabb
keywords: Flow cytometry; Glycoprotein Ib; Hemodialysis; IIb/IIIa; P-selectin; Platelets