title: Evaluation of Oxidative Metabolism in Leukocytes during Phagocytosis of Escherichia coli Carrying Genetic Constructs soxS::lux or katG::lux.
abstract: We studied ROS generation by human peripheral blood monocytes and granulocytes during phagocytosis of Escherichia coli soxS::lux or katG::lux responding by luminescence (bioluminescence) to the development of oxidative stress. Initially high sensitivity of the bioluminescent reaction of E. coli katG::lux strain to the effects of model ROS (KO2 and H2O2) and pronounced induction of luminescence upon contact with granulocytes, whereas E. coli soxS::lux demonstrated less pronounced reaction to chemical oxidants and bioluminescence was observed primarily upon contact with monocytes. A correlation was found between quantitative characteristics of E. coli katG::lux bioluminescence and luminol-dependent chemiluminescence of leukocytes in some patients, but no dependence of this kind was noted for E. coli soxS::lux. The results can provide experimental substantiation of a new approach for evaluation of ROS production by leukocytes during phagocytosis and choosing the optimal object for these studies.
authors.affiliation: Orenburg State University, Orenburg, Russia.
authors.forename: I F
authors.initials: IF
authors.lastname: Karimov
authors.affiliation: State Research Center of Dermatology and Cosmetology, Ministry of Health of the Russian Federation, Moscow, Russia. dgderyabin@yandex.ru.
authors.forename: D G
authors.initials: DG
authors.lastname: Deryabin
authors.affiliation: Orenburg State University, Orenburg, Russia.
authors.forename: D N
authors.initials: DN
authors.lastname: Karimova
authors.affiliation: Orenburg State University, Orenburg, Russia.
authors.forename: T Yu
authors.initials: TY
authors.lastname: Subbotina
authors.affiliation: State Research Institute of Genetics, Moscow, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Manukhov
mesh_terms: D002374:Catalase / Q000235:genetics*; D002478:Cells, Cultured; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D054884:Host-Pathogen Interactions; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology / Q000502:physiology*; D018384:Oxidative Stress; D010587:Phagocytosis; D017382:Reactive Oxygen Species / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D017382:Reactive Oxygen Species; D015534:Trans-Activators; C070725:SoxS protein, E coli; D002374:Catalase; C051253:katG protein, E coli
keywords: bioluminescence; katG; phagocytosis; reactive oxygen species; soxS