title: Different N-terminal isoforms of Oct-1 control expression of distinct sets of genes and their high levels in Namalwa Burkitt's lymphoma cells affect a wide range of cellular processes.
abstract: Oct-1 transcription factor has various functions in gene regulation. Its expression level is increased in several types of cancer and is associated with poor survival prognosis. Here we identified distinct Oct-1 protein isoforms in human cells and compared gene expression patterns and functions for Oct-1A, Oct-1L, and Oct-1X isoforms that differ by their N-terminal sequences. The longest isoform, Oct-1A, is abundantly expressed and is the main Oct-1 isoform in most of human tissues. The Oct-1L and the weakly expressed Oct-1X regulate the majority of Oct-1A targets as well as additional sets of genes. Oct-1X controls genes involved in DNA replication, DNA repair, RNA processing, and cellular response to stress. The high level of Oct-1 isoforms upregulates genes related to cell cycle progression and activates proliferation both in Namalwa Burkitt's lymphoma cells and primary human fibroblasts. It downregulates expression of genes related to antigen processing and presentation, cytokine-cytokine receptor interaction, oxidative metabolism, and cell adhesion, thus facilitating pro-oncogenic processes.
authors.affiliation: Department of Transcription Factors, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow 119991 Russia pank@eimb.ru.
authors.forename: Elizaveta V
authors.initials: EV
authors.lastname: Pankratova
authors.affiliation: Department of Transcription Factors, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow 119991 Russia.
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Stepchenko
authors.affiliation: Department of Transcription Factors, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow 119991 Russia.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Portseva
authors.affiliation: Department of Transcription Factors, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow 119991 Russia.
authors.forename: Vladic A
authors.initials: VA
authors.lastname: Mogila
authors.affiliation: Department of Transcription Factors, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow 119991 Russia sonjag@molbiol.edu.ru.
authors.forename: Sofia G
authors.initials: SG
authors.lastname: Georgieva
mesh_terms: D017398:Alternative Splicing; D017209:Apoptosis / Q000235:genetics; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D005347:Fibroblasts; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006019:Glycolysis; D006801:Humans; D050811:Octamer Transcription Factor-1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D054730:Protein Interaction Domains and Motifs; D020033:Protein Isoforms
chemical_list: D050811:Octamer Transcription Factor-1; D020033:Protein Isoforms