title: In Silico Meets In Vivo: Towards Computational CRISPR-Based sgRNA Design.
abstract: CRISPR-based genome editing has been widely implemented in various cell types. In silico single guide RNA (sgRNA) design is a key step for successful gene editing using the CRISPR system, and continuing efforts are aimed at refining in silico sgRNA design with high on-target efficacy and reduced off-target effects. Many sgRNA design tools are available, but careful assessments of their application scenarios and performance benchmarks across different types of genome-editing data are needed. Efficient in silico models can be built that integrate current heterogeneous genome-editing data to derive unbiased sgRNA design rules and identify key features for improving sgRNA design. Comprehensive evaluation of on-target and off-target effects of sgRNA will allow more precise genome editing and gene therapies using the CRISPR system.
authors.affiliation: Department of Central Laboratory, Shanghai Tenth People's Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China.
authors.forename: Guo-Hui
authors.initials: GH
authors.lastname: Chuai
authors.affiliation: Department of Clinical Oncology, Huai'an First People's Hospital, Nanjing Medical University, China.
authors.forename: Qi-Long
authors.initials: QL
authors.lastname: Wang
authors.affiliation: Department of Central Laboratory, Shanghai Tenth People's Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China. Electronic address: qiliu@tongji.edu.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D003198:Computer Simulation; D000072669:Gene Editing / Q000379:methods*; D018390:Gene Targeting / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008957:Models, Genetic; D017393:RNA Editing / Q000235:genetics*; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems
keywords: CRISPR; genome editing; in silico sgRNA design; off-target