title: A comparative proteomic study on the effects of metal pollution in oysters Crassostrea hongkongensis.
abstract: The metal pollution has posed great risk on the coastal organisms along the Jiulongjiang Estuary in South China. In this work, two-dimensional electrophoresis-based proteomics was applied to the oysters Crassostrea hongkongensis from metal pollution sites to characterize the proteomic responses to metal pollution. Metal accumulation and proteomic responses indicated that the oysters from BJ site were more severely contaminated than those from FG site. Compared with those oyster samples from the clean site (JZ), metal pollution induced cellular injuries, oxidative and immune stresses in oyster heapatopancreas from both BJ and FG sites via differential metabolic pathways. In addition, metal pollution in BJ site induced disturbance in energy and lipid metabolisms in oysters. Results indicated that cathepsin L and ferritin GF1 might be the biomarkers of As and Fe in oyster C. hongkongensis, respectively. This study demonstrates that proteomics is a useful tool for investigating biological effects induced by metal pollution.
authors.affiliation: Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, P.R. China; University of Chinese Academy of Sciences, Beijing 100049, P.R. China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, P.R. China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Ji
authors.affiliation: Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, P.R. China. Electronic address: hfwu@yic.ac.cn.
authors.forename: Huifeng
authors.initials: H
authors.lastname: Wu
authors.affiliation: Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of Environment and Ecology, Xiamen University, Xiamen 361102, P.R. China.
authors.forename: Qiaoguo
authors.initials: Q
authors.lastname: Tan
authors.affiliation: Division of Life Science, The Hong Kong University of Science and Technology (HKUST), Clearwater Bay, Kowloon, Hong Kong.
authors.forename: Wen-Xiong
authors.initials: WX
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002681:China; D049896:Crassostrea / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004784:Environmental Monitoring / Q000379:methods*; D004787:Environmental Pollution / Q000032:analysis; D063366:Estuaries; D005843:Geography; D008670:Metals / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D012758:Shellfish; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D015415:Biomarkers; D008670:Metals; D020543:Proteome; D014874:Water Pollutants, Chemical
keywords: Crassostrea hongkongensis; Hepatopancreas; Metal pollution; Proteomics