title: Effects of the -791(C→T) mutation in the promoter for tumor necrosis factor alpha on gene expression and resistance of Large White pigs to enterotoxigenic Escherichia coli F18.
abstract: Tumor necrosis factor alpha (TNF-α) plays an important role in the immune system. In this study, TNF-α expression was analyzed in 11 tissues of 8 piglets resistant to enterotoxigenic Escherichia coli (ETEC) F18 and 8 ETEC F18-susceptible piglets from the Large White breed. The expression levels of TNF-α were high in immune organs (spleen, lung, thymus, and lymph nodes). The levels were higher in ETEC F18-resistant piglets than in ETEC F18-susceptible piglets, with significant differences in spleen, kidney, thymus, lymph node, and duodenum (P < 0.05). The mutation TNF-α -791(C→T) and 3 genotypes (CC, CT, and TT) were identified. The TNF-α expression levels in the spleen, kidney, lymph nodes, and duodenum were significantly higher in the TT pigs than in the CC pigs (P < 0.05). Thus, TNF-α -791(C→T) has significant effects on mRNA expression and may regulate ETEC F18 resistance of weaning piglets. Therefore, the -791(C→T) mutation of the TNF-α gene could be considered an important potential genetic marker of ETEC F18 resistance.
authors.affiliation: Department of College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China (Liu, Dai, Sun, Wu, Bao); Department of College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China (Zhu).
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China (Liu, Dai, Sun, Wu, Bao); Department of College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China (Zhu).
authors.forename: Chaohui
authors.initials: C
authors.lastname: Dai
authors.affiliation: Department of College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China (Liu, Dai, Sun, Wu, Bao); Department of College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China (Zhu).
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China (Liu, Dai, Sun, Wu, Bao); Department of College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China (Zhu).
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Department of College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China (Liu, Dai, Sun, Wu, Bao); Department of College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China (Zhu).
authors.forename: Shenglong
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China (Liu, Dai, Sun, Wu, Bao); Department of College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China (Zhu).
authors.forename: Wenbin
authors.initials: W
authors.lastname: Bao
mesh_terms: D000818:Animals; D054307:Enterotoxigenic Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D005786:Gene Expression Regulation / Q000276:immunology; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000235:genetics / Q000382:microbiology*; D014018:Tissue Distribution; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D005819:Genetic Markers; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha