title: A Label-Free Immunosensor for CEA Based on Pd-Ir Bimetallic Nanoparticles.
abstract: In this paper, a kind of label-free immunosensor for rapid detection of carcinoembryonic antigen (CEA) based on Pd-Ir bimetallic nanoparticles as a catalyst was prepared. CEA antibody (anti-CEA) was immobilized onto the surface of glassy carbon electrode through the adsorption effect of Pd-Ir bimetallic nanoparticles modified on the electrode using chitosan. When CEA antibody combines with CEA antigen, the formed immunocomplexs blocks the catalytic activity of Pd-Ir bimetal nanoparticles to the reduction of H2O2, leading to decrease in the current response signal. The decrease of the current response of the immunosensor has a linear relationship with the concentration of CEA ranging from 0.05 ng/mL to 50 ng/mL with a detection limit of 0.017 ng/mL at 3σ. The sensor provides a new simple method for the determination of CEA.
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Niu
authors.forename: Jinhang
authors.initials: J
authors.lastname: Zhao
authors.forename: Tingzhi
authors.initials: T
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Yang