title: Tetracycline-incorporated polymer nanofibers as a potential dental implant surface modifier.
abstract: This study investigated the antimicrobial and osteogenic properties of titanium (Ti) disks superficially modified with tetracycline (TCH)-incorporated polymer nanofibers. The experiments were carried out in two phases. The first phase dealt with the synthesis and characterization (i.e., morphology, mechanical strength, drug release, antimicrobial activity, and cytocompatibility) of TCH-incorporated fibers. The second phase was dedicated to evaluating both the antimicrobial and murine-derived osteoprecursor cell (MC3T3-E1) response of Ti-modified with TCH-incorporated fibers. TCH was successfully incorporated into the submicron-sized and cytocompatible fibers. All TCH-incorporated mats presented significant antimicrobial activity against periodontal pathogens. The antimicrobial potential of the TCH-incorporated fibers-modified Ti was influenced by both the TCH concentration and bacteria tested. At days 5 and 7, a significant increase in MC3T3-E1 cell number was observed for TCH-incorporated nanofibers-modified Ti disks when compared to that of TCH-free nanofibers-modified Ti-disks and bare Ti. A significant increase in alkaline phosphatase (ALP) levels on the Ti disks modified with TCH-incorporated nanofiber on days 7 and 14 was seen, suggesting that the proposed surface promotes early osteogenic differentiation. Collectively, the data suggest that TCH-incorporated nanofibers could function as an antimicrobial surface modifier and osteogenic inducer for Ti dental implants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2085-2092, 2017.
authors.affiliation: Department of Biomedical and Applied Sciences, Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana, 46202.|Department of Biomedical Engineering, Indiana University Purdue University, Indianapolis, Indiana, 46202.|Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, Indiana, 46202.
authors.forename: Marco C
authors.initials: MC
authors.lastname: Bottino
authors.affiliation: Department of Biomedical and Applied Sciences, Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana, 46202.
authors.forename: Eliseu A
authors.initials: EA
authors.lastname: Münchow
authors.affiliation: Department of Biomedical and Applied Sciences, Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana, 46202.
authors.forename: Maria T P
authors.initials: MTP
authors.lastname: Albuquerque
authors.affiliation: Department of Biomedical and Applied Sciences, Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana, 46202.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kamocki
authors.affiliation: Department of Biomedical and Applied Sciences, Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana, 46202.
authors.forename: Rana
authors.initials: R
authors.lastname: Shahi
authors.affiliation: Department of Biomedical and Applied Sciences, Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana, 46202.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Gregory
authors.affiliation: Department of Biomedical and Applied Sciences, Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana, 46202.
authors.forename: Tien-Min G
authors.initials: TG
authors.lastname: Chu
authors.affiliation: Department of Biomedical and Applied Sciences, Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana, 46202.
authors.forename: Divya
authors.initials: D
authors.lastname: Pankajakshan
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D015921:Dental Implants; D006090:Gram-Negative Bacteria / Q000254:growth & development*; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D013499:Surface Properties; D013752:Tetracycline / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D014025:Titanium / Q000737:chemistry*
chemical_list: D015921:Dental Implants; D014025:Titanium; D013752:Tetracycline
keywords: antimicrobial; coating; electrospinning; nanofibers; osteoblasts; tetracycline; titanium