title: Comparative in vitro study of the effectiveness of Green tea extract and common storage media on periodontal ligament fibroblast viability.
abstract: OBJECTIVE
  Green tea extract (GTE) was shown to be effective in preserving periodontal ligament fibroblasts (PDLFs) of avulsed teeth. This study aimed at determining the potential of GTE in preserving the viability of PDLFs comparing with different storage media.
  
  
  METHODS
  Periodontal ligament cells were obtained from freshly extracted healthy impacted third molars and cultured in Dulbecco's Modified Eagle Medium (DMEM). Cell viability was determined by storing the cells in seven media; DMEM, tap water, Hank's balanced salt solution (HBSS), whole milk, hypotonic sucrose solution, GTE, and GTE + sucrose for 1, 2, 4, and 24 h at 37°C using tetrazolium salt-based colorimetric (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay. Statistical analysis was performed by one-way analysis of variance and post hoc tests.
  
  
  RESULTS
  GTE showed significantly higher protective effect than HBSS at 2, 4, and 24 h (P = 0.009, P = 0.02, P = 0.016), DMED at 2 h (P = 0.003), and milk at 4 h (P = 0.039).
  
  
  CONCLUSIONS
  Although with undesirable osmolality and pH, GTE had a good ability in preserving the PDLFs comparing with other studied media.
authors.affiliation: Student's Research Committee, Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Adeli
authors.affiliation: Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Zabihi
authors.affiliation: Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Abedian
authors.affiliation: Department of Pediatrics, Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Samane
authors.initials: S
authors.lastname: Gharekhani
authors.affiliation: Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Pouramir
authors.affiliation: Department of Biostatics, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Soraya
authors.initials: S
authors.lastname: Khafri
authors.affiliation: Department of Pediatrics, Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ghasempour
keywords: Avulsion; Green tea extract; cell viability; periodontal ligament cell