title: pH-dependent conformational changes of a Thogoto virus matrix protein reveal mechanisms of viral assembly and uncoating.
abstract: Orthomyxoviruses are a family of ssRNA virus, including influenza virus, infectious salmon anaemia virus and Thogoto virus. The matrix proteins of orthomyxoviruses play crucial roles in some essential processes of the viral life cycle. However, the mechanisms of the matrix proteins involved in these processes remain incompletely understood. Currently, only the structure and function of the matrix protein from influenza virus have been studied. Here, we present the crystal structures of the N-terminal domain of matrix protein from Thogoto virus at pH 7.0 and 4.5. By analysing the structures, we identified the conformational changes of monomers and dimers in different pH conditions, mainly caused by two flexible loops, L3 and L5. These structural deviations would reflect the basis of viral capsid assembly or disassembly.
authors.affiliation: State Key Laboratory of Virology/Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, PR China.|National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Mingrui
authors.initials: M
authors.lastname: Yang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Virology/Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, PR China.|National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yingfang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology/Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, PR China.
authors.forename: Zhanqiu
authors.initials: Z
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Virology/Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Liang
mesh_terms: D018360:Crystallography, X-Ray; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D011487:Protein Conformation; D018117:Thogotovirus / Q000502:physiology*; D014763:Viral Matrix Proteins / Q000737:chemistry*; D019065:Virus Assembly; D057646:Virus Uncoating
chemical_list: D014763:Viral Matrix Proteins