title: Indoor pollutant hexabromocyclododecane enhances house dust mite-induced activation of human monocyte-derived dendritic cells.
abstract: The indoor pollutant hexabromocyclododecane (HBCD) has been added as flame retardant to many consumer products but detaches and accumulates in house dust. Inhalation of house dust leads to exposure to house dust mite (HDM) allergens in the presence of HBCD. Activation of dendritic cells is crucial in the sensitization to HDM allergens. The current study examined whether exposure to HBCD affected activation/maturation of HDM-exposed human dendritic cells (DC). Human monocyte-derived DC (moDC) were exposed simultaneously to HDM and a concentration range of HBCD (0.1-20 μM) in vitro. HDM exposure of moDC induced expression of co-stimulatory molecule CD80 and production of pro-inflammatory cytokines interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α. However, simultaneous exposure of moDC to HBCD and HDM enhanced the expression of antigen presenting molecule HLA-DR, co-stimulatory molecule CD86 and pro-inflammatory cytokine IL-8 depending on the dose of HBCD. Our results indicate that simultaneous exposure of HDM and HBCD can enhance the antigen presentation and maturation/activation of DC.
authors.affiliation: a Department of Experimental Immunology, Academic Medical Centre , University of Amsterdam , Amsterdam , the Netherlands.
authors.forename: Derya
authors.initials: D
authors.lastname: Canbaz
authors.affiliation: a Department of Experimental Immunology, Academic Medical Centre , University of Amsterdam , Amsterdam , the Netherlands.
authors.forename: M Cristina
authors.initials: MC
authors.lastname: Lebre
authors.affiliation: a Department of Experimental Immunology, Academic Medical Centre , University of Amsterdam , Amsterdam , the Netherlands.
authors.forename: Adrian
authors.initials: A
authors.lastname: Logiantara
authors.affiliation: a Department of Experimental Immunology, Academic Medical Centre , University of Amsterdam , Amsterdam , the Netherlands.|b Department of Otorhinolaryngology, Academic Medical Centre , University of Amsterdam , Amsterdam , the Netherlands.
authors.forename: Ronald
authors.initials: R
authors.lastname: van Ree
authors.affiliation: a Department of Experimental Immunology, Academic Medical Centre , University of Amsterdam , Amsterdam , the Netherlands.
authors.forename: Leonie S
authors.initials: LS
authors.lastname: van Rijt
mesh_terms: D016902:Air Pollution, Indoor / Q000009:adverse effects; D000818:Animals; D017951:Antigen Presentation; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D005411:Flame Retardants; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D006842:Hydrocarbons, Brominated / Q000276:immunology*; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D009000:Monocytes / Q000276:immunology; D039981:Pyroglyphidae / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D039741:Antigens, Dermatophagoides; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D005411:Flame Retardants; D006684:HLA-DR Antigens; D006842:Hydrocarbons, Brominated; D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; C089796:hexabromocyclododecane
keywords: Allergy; hexabromocyclododecane; house dust mite; human; monocyte-derived dendritic cells