title: Dual binding mode of antithyroid drug methimazole to mammalian heme peroxidases - structural determination of the lactoperoxidase-methimazole complex at 1.97 Å resolution.
abstract: Lactoperoxidase (LPO, EC 1.11.1.7) is a member of the mammalian heme peroxidase family which also includes thyroid peroxidase (TPO). These two enzymes have a sequence homology of 76%. The structure of LPO is known but not that of TPO. In order to determine the mode of binding of antithyroid drugs to thyroid peroxidase, we have determined the crystal structure of LPO complexed with an antithyroid drug, methimazole (MMZ) at 1.97 Å resolution. LPO was isolated from caprine colostrum, purified to homogeneity and crystallized with 20% poly(ethylene glycol)-3350. Crystals of LPO were soaked in a reservoir solution containing MMZ. The structure determination showed the presence of two crystallographically independent molecules in the asymmetric unit. Both molecules contained one molecule of MMZ, but with different orientations. MMZ was held tightly between the heme moiety on one side and the hydrophobic parts of the side chains of Arg255, Glu258, and Leu262 on the opposite side. The back of the cleft contained the side chains of Gln105 and His109 which also interacted with MMZ. In both orientations, MMZ had identical buried areas and formed a similar number of interactions. It appears that the molecules of MMZ can enter the substrate-binding channel of LPO in two opposite orientations. But once they reach the distal heme pocket, their orientations are frozen due to equally tight packing of MMZ in both orientations. This is a novel example of an inhibitor binding to an enzyme with two orientations at the same site with nearly equal occupancies.
authors.affiliation: Department of Biophysics All India Institute of Medical Sciences New Delhi India.
authors.forename: Rashmi Prabha
authors.initials: RP
authors.lastname: Singh
authors.affiliation: Department of Biophysics All India Institute of Medical Sciences New Delhi India.
authors.forename: Avinash
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Biophysics All India Institute of Medical Sciences New Delhi India.
authors.forename: Harsh Vardhan
authors.initials: HV
authors.lastname: Sirohi
authors.affiliation: Department of Biophysics All India Institute of Medical Sciences New Delhi India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Department of Biophysics All India Institute of Medical Sciences New Delhi India.
authors.forename: Punit
authors.initials: P
authors.lastname: Kaur
authors.affiliation: Department of Biophysics All India Institute of Medical Sciences New Delhi India.
authors.forename: Sujata
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Biophysics All India Institute of Medical Sciences New Delhi India.
authors.forename: Tej P
authors.initials: TP
authors.lastname: Singh
keywords: antithyroid drug; crystal structure; distal heme pocket; lactoperoxidase; mammalian heme peroxidases; methimazole