title: Bacterial communities associated with sulfonamide antibiotics degradation in sludge-amended soil.
abstract: This study investigated the degradation of sulfonamide antibiotics (SAs) and microbial community changes in sludge-amended soil. In batch experiments, SA degradation was enhanced by addition of spent mushroom compost (SMC), SMC extract, and extract-containing microcapsule, with SMC showing higher SA degradation rate than the other additives in soil-sludge mixtures. In bioreactor experiments, the degradation of SAs in soil-sludge mixtures was in the order of sulfamethoxazole > sulfadimethoxine > sulfamethazine during four times of SA addition. SA removal was higher in soil-sludge mixtures than in soil alone. The bacterial composition differed in soil-sludge mixtures with and without SMC. In total, 44 differentially distributed bacterial genera were identified from different experimental settings and stages. Four bacterial genera, Acinetobacter, Alcaligenes, Brevundimonas, and Pseudomonas, were previously found involved in SA degradation, and 20 of the 44 bacterial genera were previously found in aromatic hydrocarbon degradation. Therefore, these bacteria have high potential to be SA degradation bacteria in this study.
authors.affiliation: Department of Microbiology, Soochow University, Taipei, Taiwan.
authors.forename: Chu-Wen
authors.initials: CW
authors.lastname: Yang
authors.affiliation: Department of Microbiology, Soochow University, Taipei, Taiwan.
authors.forename: Wan-Chun
authors.initials: WC
authors.lastname: Hsiao
authors.affiliation: Department of Microbiology, Soochow University, Taipei, Taiwan.
authors.forename: Chu-Hsih
authors.initials: CH
authors.lastname: Fan
authors.affiliation: Department of Microbiology, Soochow University, Taipei, Taiwan. bvchang@scu.edu.tw.
authors.forename: Bea-Ven
authors.initials: BV
authors.lastname: Chang
mesh_terms: D000363:Agaricales; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D019149:Bioreactors; D012722:Sewage / Q000382:microbiology*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D013412:Sulfadimethoxine / Q000378:metabolism*; D013418:Sulfamethazine / Q000378:metabolism*; D013420:Sulfamethoxazole / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D012722:Sewage; D012989:Soil Pollutants; D013412:Sulfadimethoxine; D013418:Sulfamethazine; D013420:Sulfamethoxazole
keywords: Bacterial community; Degradation; Sludge-amended soil; Spent mushroom compost; Sulfonamide antibiotics