title: Amelioration of EAE by a cryptic epitope of myelin oligodendrocyte glycoprotein.
abstract: Previous work demonstrated that EAE induced by recombinant human MOG was B cell-dependent. Data presented here reveal a T cell response to MOG61-85 in human rMOG-immunized B cell-/-mice not observed in WT mice. Further study revealed this peptide to be a cryptic epitope in WT mice. Co-immunization of B cell-/-mice with MOG35-55 and MOG61-85 peptides led to less severe disease compared to mice immunized with MOG35-55 alone. Disease amelioration was associated with decreased production of Interferon-γ by lymph node cells. Thus, MOG61-85 represents a protective epitope to human rMOG induced EAE in B cell-/-mice.
authors.affiliation: Department of Biomedical Sciences, University of Wisconsin - Milwaukee, Milwaukee, WI 53211, USA; Department of Neurology and Neurosurgery, Washington University School of Medicine, Saint Louis, MO, USA 63110. Electronic address: jlyons@uwm.edu.
authors.forename: Jeri A
authors.initials: JA
authors.lastname: Lyons
authors.affiliation: Department of Biomedical Sciences, University of Wisconsin - Milwaukee, Milwaukee, WI 53211, USA.
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Riter
authors.affiliation: Department of Biomedical Sciences, University of Wisconsin - Milwaukee, Milwaukee, WI 53211, USA.
authors.forename: Alaa M
authors.initials: AM
authors.lastname: Almatrook
authors.affiliation: Department of Neurology and Neurosurgery, Washington University School of Medicine, Saint Louis, MO, USA 63110.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Ramsbottom
authors.affiliation: Department of Neurology and Neurosurgery, Washington University School of Medicine, Saint Louis, MO, USA 63110.
authors.forename: Anne H
authors.initials: AH
authors.lastname: Cross
mesh_terms: D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology*; D000939:Epitopes / Q000008:administration & dosage* / Q000276:immunology*; D005260:Female; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D000939:Epitopes; D063308:Myelin-Oligodendrocyte Glycoprotein
keywords: Antibody; B cells; Experimental autoimmune encephalomyelitis; Immune regulation; Myelin oligodendrocyte glycoprotein; T cell epitopes