title: Deciphering bacterial community changes in zucker diabetic fatty rats based on 16S rRNA gene sequences analysis.
abstract: The aim of the present pilot study was deciphering bacterial community changes in Zucker diabetic fatty rats (ZDF rats), a model of type 2 diabetes. Recent studies unmasked that the status of gastrointestinal tract microbiota has a marked impact on nutrition-related syndromes such as obesity and type-2 diabetes (T2D). In this study, samples taken from the gastrointestinal tracts (GI tracts) of ZDF and their lean littermates (ZL rats) were subjected to 16S rRNA gene sequence-based analysis to examine the characteristic bacterial communities, including those located in the stomach, duodenum, jejunum, ileum, cecum and feces. Results revealed that the Firmicutes/Bacteroidetes ratio was increased and greater numbers of Lactobacillus were detected along GI tracts in ZDF rats compared to ZL rats. In conclusion, this work is the first study to systematically characterize bacterial communities along ZDF rat GI tract and provides substantial evidence supporting a prospective strategy to alter the GI microbial communities improving obesity and T2D.
authors.affiliation: Basic Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Gu
authors.affiliation: Basic Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yang
authors.affiliation: The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xiang
authors.affiliation: The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Liang
authors.affiliation: Institute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Sui
authors.affiliation: Basic Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Libin
authors.initials: L
authors.lastname: Zhan
authors.affiliation: Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, Liaoning, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Lu
mesh_terms: D000818:Animals; D041963:Bacteroidetes; D001786:Blood Glucose; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000382:microbiology*; D003924:Diabetes Mellitus, Type 2 / Q000382:microbiology*; D000068536:Bacillota; D000069196:Gastrointestinal Microbiome; D041981:Gastrointestinal Tract / Q000382:microbiology; D007778:Lactobacillus; D008297:Male; D020125:Mutation, Missense; D009765:Obesity; D010865:Pilot Projects; D011446:Prospective Studies; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats; D011924:Rats, Zucker; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D001786:Blood Glucose; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; Pathology Section; gastrointestinal microbe; microbial communities; type 2 diabetes; zucker diabetic fatty rat