title: [Expression profiles of PI3K, NF-κB, and STAT1 in peripheral blood mononuclear cells in children with bronchial asthma].
abstract: To study the expression profiles of PI3K, NF-κB, and STAT1 in peripheral blood mononuclear cells (PBMCs) in children with bronchial asthma, as well as their roles in the pathogenesis of asthma.
  
  
  
  Thirty children with acute exacerbation of bronchial asthma were enrolled as the asthma group, and 20 healthy children were enrolled as the control group. RT-PCR and Western blot were used to measure the mRNA and protein expression levels of PI3K, NF-κB, and STAT1 in PBMCs. A spirometer was used to compare the pulmonary function between the two groups. The correlations between the mRNA expression of PI3K, NF-κB, and STAT1 and pulmonary function in children with bronchial asthma were analyzed.
  
  
  
  The asthma group had significantly higher mRNA and protein expression levels of PI3K, NF-κB, and STAT1 than the control group (P<0.05). Compared with the control group, the asthma group showed significant reductions in pulmonary function indices such as FEV1%, FEV1/FVC, and PEF% (P<0.05). In children with bronchial asthma, the mRNA expression levels of PI3K, NF-κB, and STAT1 were negatively correlated with FEV1%, FEV1/FVC, and PEF% (P<0.05).
  
  
  
  The expression levels of PI3K, NF-κB, and STAT1 increase in children with asthma, and are negatively correlated with pulmonary function indices, suggesting that PI3K, NF-κB and STAT1 are involved in the development and progression of bronchial asthma in children.
authors.affiliation: Department of Pediatrics, Shenzhen Luohu People's Hospital, Shenzhen, Guangdong 518001, China. lyyylap@163.com.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Shi
authors.forename: Jie-Bo
authors.initials: JB
authors.lastname: Liu
authors.forename: Ai-Pin
authors.initials: AP
authors.lastname: Lu
mesh_terms: D001249:Asthma / Q000097:blood / Q000209:etiology* / Q000503:physiopathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D008297:Male; D016328:NF-kappa B / Q000097:blood / Q000235:genetics / Q000502:physiology*; D019869:Phosphatidylinositol 3-Kinases / Q000097:blood / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D050794:STAT1 Transcription Factor / Q000097:blood / Q000235:genetics / Q000502:physiology*
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D019869:Phosphatidylinositol 3-Kinases