title: Reconstruction of metabolic networks in a fluoranthene-degrading enrichments from polycyclic aromatic hydrocarbon polluted soil.
abstract: Microbial degradation of polycyclic aromatic hydrocarbons (PAHs) is the primary process of removing PAHs from environments. The metabolic pathway of PAHs in pure cultures has been intensively studied, but cooperative metabolisms at community-level remained to be explored. In this study, we determined the dynamic composition of a microbial community and its metabolic intermediates during fluoranthene degradation using high-throughput metagenomics and gas chromatography-mass spectrometry (GC-MS), respectively. Subsequently, a cooperative metabolic network for fluoranthene degradation was constructed. The network shows that Mycobacterium contributed the majority of ring-hydroxylating and -cleavage dioxygenases, while Diaphorobacter contributed most of the dehydrogenases. Hyphomicrobium, Agrobacterium, and Sphingopyxis contributed to genes encoding enzymes involved in downstream reactions of fluoranthene degradation. The contributions of various microbial groups were calculated with the PICRUSt program. The contributions of Hyphomicrobium to alcohol dehydrogenases were 62.4% in stage 1 (i.e., when fluoranthene was rapidly removed) and 76.8% in stage 3 (i.e., when fluoranthene was not detectable), respectively; the contribution of Pseudomonas were 6.6% in stage 1 and decreased to 1.2% in subsequent stages. To the best of the author's knowledge, this report describes the first cooperative metabolic network to predict the contributions of various microbial groups during PAH-degradation at community-level.
authors.affiliation: State Key Laboratory of Microbial Resources at Institute of Microbiology, CAS, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Microbial Resources at Institute of Microbiology, CAS, Beijing 100101, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Resources at Institute of Microbiology, CAS, Beijing 100101, China.
authors.forename: Guo-Min
authors.initials: GM
authors.lastname: Ai
authors.affiliation: Research Center of Ecological and Environmental Science, CAS, Beijing, China; IMCAS-RCEECAS Joint-Lab of Microbial Technology for Environmental Science, Beijing, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Microbial Resources at Institute of Microbiology, CAS, Beijing 100101, China; IMCAS-RCEECAS Joint-Lab of Microbial Technology for Environmental Science, Beijing, China. Electronic address: liusj@im.ac.cn.
authors.forename: Shuang-Jiang
authors.initials: SJ
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources at Institute of Microbiology, CAS, Beijing 100101, China; IMCAS-RCEECAS Joint-Lab of Microbial Technology for Environmental Science, Beijing, China. Electronic address: jiangcy@im.ac.cn.
authors.forename: Cheng-Ying
authors.initials: CY
authors.lastname: Jiang
mesh_terms: D001419:Bacteria / Q000201:enzymology / Q000378:metabolism; D001673:Biodegradation, Environmental; D004787:Environmental Pollution; D005449:Fluorenes / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D053858:Metabolic Networks and Pathways; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005449:Fluorenes; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; C007738:fluoranthene
keywords: Fluoranthene biodegradation; Metabolic networks; Microbial community; PICRUSt; Polycyclic aromatic hydrocarbons (PAHs)