title: Effect of Interleukin-1 α and Interleukin-1 β on Erythroid Progenitor Cell Growth in Serum Free Cultures: An In Vitro Study Relevant to the Pathogenesis of the Anemia of Chronic Disease.
abstract: It has been postulated that interleukin-lα (IL-lα) and interleukin-1β (IL-1β) play a role in the pathogenesis of the anemia of chronic disease by inhibiting the proliferation of human erythroid progenitor cells. In the course of investigating this hypothesis we found that IL-1 type I receptor (IL-1R) mRNA is expressed on erythroid progenitor enriched, primitive human hematopoietic cells (CD34+, c-kit-R(bright)) and on cells isolated from human erythroid burst forming colonies (BFU-E). Nevertheless, when CD34+, c-kit-R(bright) cells were exposed to IL-1α and IL-1β in vitro, cloning efficacy of BFU-E and CFU-E in a serum free culture system was not inhibited. Moreover, in apparent contradiction to the hypothesis being tested, we found that both IL-1 isoforms actually increased the survival of human BFU-E in serum free, growth factor free medium. Accordingly, these results suggest that if IL-1 plays a role in the pathogenesis of the anemia of chronic disease, it is not due to a direct suppressive effect on erythroid cell growth. Rather, our data support the hypothesis that IL-1 may cause the elaboration of another inhibitory cytokine (s) by cells of the marrow microenvironment.
authors.affiliation: a Departments of Pathology University of Pennsylvania School of Medicine , Philadelphia , PA.
authors.forename: J
authors.initials: J
authors.lastname: Ratajczak
authors.affiliation: a Departments of Pathology University of Pennsylvania School of Medicine , Philadelphia , PA.
authors.forename: W
authors.initials: W
authors.lastname: Marlicz
authors.affiliation: c Department of Pathology Germantown Friends School , Philadelphia , PA.
authors.forename: A
authors.initials: A
authors.lastname: Keidel
authors.affiliation: a Departments of Pathology University of Pennsylvania School of Medicine , Philadelphia , PA.
authors.forename: B
authors.initials: B
authors.lastname: Machalinski
authors.affiliation: a Departments of Pathology University of Pennsylvania School of Medicine , Philadelphia , PA.
authors.forename: M Z
authors.initials: MZ
authors.lastname: Ratajczak
authors.affiliation: b Department of Pathology Internal Medicine , University of Pennsylvania School of Medicine , Philadelphia , PA.
authors.forename: A M
authors.initials: AM
authors.lastname: Gewirtz
keywords: Anemia; Erythropoiesis; Hemopoiesis; Interleukin-1; Interleukin-1 Receptor