title: Survival and Reduction of Shiga Toxin-Producing Escherichia coli in a Fresh Cold-Pressed Juice Treated with Antimicrobial Plant Extracts.
abstract: This study was conducted to evaluate the survival of 7 Shiga-toxigenic Escherichia coli (STEC) in fresh cold-pressed juice and the antimicrobial efficacy of 4 essential oils (EO: achillea, rosemary, sage, and thyme). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each EO was determined using microdilution assays evaluated at pH levels 4 and 7; as well as at 4 and 25 °C; daily for up to 5 d. Results indicated that 5 of 7 serotypes survived well in cold-pressed raw juice for at least 4 d at 4 °C and pH 3.5 with no significant (P > 0.05) reduction in viability. The EO showed varying degrees of antimicrobial activity against the 7 STEC. The MIC and MBCs were lowest for thyme (2 μg/L) and highest for sage (15 to 25 μg/L). The antimicrobial activity was enhanced at low pH and temperature. Data showed that although the top 7 STEC could survive low pH and temperature in vitro and in cold-pressed juices, EO, especially from thyme and rosemary, reduced STEC to an undetectable level at 4 °C, suggesting that they could be used as natural antimicrobials in juice.
authors.affiliation: College of Environmental Agricultural Sciences, Suez Canal Univ, Al-Arish, North Sinai, 45516, Egypt.|College of Food Science and Pharmaceutics, Zhejiang Ocean Univ, Zhoushan, 316022, China.
authors.forename: Shaimaa
authors.initials: S
authors.lastname: Hatab
authors.affiliation: Food Science Dept, Univ. of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
authors.forename: Renata
authors.initials: R
authors.lastname: Athanasio
authors.affiliation: Food Science Dept, Univ. of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
authors.forename: Richard
authors.initials: R
authors.lastname: Holley
authors.affiliation: Food Science Dept, Univ. of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
authors.forename: Argenis
authors.initials: A
authors.lastname: Rodas-Gonzalez
authors.affiliation: Food Science Dept, Univ. of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
authors.forename: Claudia
authors.initials: C
authors.lastname: Narvaez-Bravo
mesh_terms: D031162:Achillea / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D003080:Cold Temperature; D019453:Escherichia coli O157 / Q000187:drug effects; D005516:Food Microbiology; D000067030:Fruit and Vegetable Juices / Q000382:microbiology*; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D027542:Rosmarinus / Q000737:chemistry; D027543:Salvia officinalis / Q000737:chemistry; D054323:Shiga-Toxigenic Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D017365:Spices; D046930:Thymus Plant / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D009822:Oils, Volatile; D010936:Plant Extracts
keywords: Shiga-toxigenic E. coli; antibacterial activity; antimicrobial; cold-pressed juice; essential oils