title: The Arabidopsis thaliana lectin receptor kinase LecRK-I.9 is required for full resistance to Pseudomonas syringae and affects jasmonate signalling.
abstract: On microbial attack, plants can detect invaders and activate plant innate immunity. For the detection of pathogen molecules or cell wall damage, plants employ receptors that trigger the activation of defence responses. Cell surface proteins that belong to large families of lectin receptor kinases are candidates to function as immune receptors. Here, the function of LecRK-I.9 (At5g60300), a legume-type lectin receptor kinase involved in cell wall-plasma membrane contacts and in extracellular ATP (eATP) perception, was studied through biochemical, gene expression and reverse genetics approaches. In Arabidopsis thaliana, LecRK-I.9 expression is rapidly, highly and locally induced on inoculation with avirulent strains of Pseudomonas syringae pv. tomato (Pst). Two allelic lecrk-I.9 knock-out mutants showed decreased resistance to Pst. Conversely, over-expression of LecRK-I.9 led to increased resistance to Pst. The analysis of defence gene expression suggests an alteration of both the salicylic acid (SA) and jasmonic acid (JA) signalling pathways. In particular, LecRK-I.9 expression during plant-pathogen interaction was dependent on COI1 (CORONATINE INSENSITIVE 1) and JAR1 (JASMONATE RESISTANT 1) components, and JA-responsive transcription factors (TFs) showed altered levels of expression in plants over-expressing LecRK-I.9. A similar misregulation of these TFs was obtained by JA treatment. This study identified LecRK-I.9 as necessary for full resistance to Pst and demonstrated its involvement in the control of defence against pathogens through a regulation of JA signalling components. The role of LecRK-I.9 is discussed with regard to the potential molecular mechanisms linking JA signalling to cell wall damage and/or eATP perception.
authors.affiliation: CNRS, Laboratoire des Interactions Plantes Microorganismes (LIPM), UMR2594, Castanet-Tolosan, 31326, France.|INRA, Laboratoire des Interactions Plantes Microorganismes (LIPM), UMR441, Castanet-Tolosan, 31326, France.
authors.forename: Claudine
authors.initials: C
authors.lastname: Balagué
authors.affiliation: CNRS, Laboratoire des Interactions Plantes Microorganismes (LIPM), UMR2594, Castanet-Tolosan, 31326, France.|INRA, Laboratoire des Interactions Plantes Microorganismes (LIPM), UMR441, Castanet-Tolosan, 31326, France.|Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS; BP 42617 Auzeville, Castanet-Tolosan, 31326, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Gouget
authors.affiliation: CNRS, Laboratoire des Interactions Plantes Microorganismes (LIPM), UMR2594, Castanet-Tolosan, 31326, France.|INRA, Laboratoire des Interactions Plantes Microorganismes (LIPM), UMR441, Castanet-Tolosan, 31326, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Bouchez
authors.affiliation: CNRS, Laboratoire des Interactions Plantes Microorganismes (LIPM), UMR2594, Castanet-Tolosan, 31326, France.|INRA, Laboratoire des Interactions Plantes Microorganismes (LIPM), UMR441, Castanet-Tolosan, 31326, France.|Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS; BP 42617 Auzeville, Castanet-Tolosan, 31326, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Souriac
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS; BP 42617 Auzeville, Castanet-Tolosan, 31326, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Haget
authors.affiliation: AgroParisTech, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Saclay Plant Science, Versailles, 78000, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Boutet-Mercey
authors.affiliation: Laboratory of Phytopathology, Plant Sciences Group, Wageningen University, Droevendaalsesteeg 1, Wageningen, PB, 6708, the Netherlands.
authors.forename: Francine
authors.initials: F
authors.lastname: Govers
authors.affiliation: CNRS, Laboratoire des Interactions Plantes Microorganismes (LIPM), UMR2594, Castanet-Tolosan, 31326, France.|INRA, Laboratoire des Interactions Plantes Microorganismes (LIPM), UMR441, Castanet-Tolosan, 31326, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Roby
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS; BP 42617 Auzeville, Castanet-Tolosan, 31326, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Canut
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D016923:Cell Death / Q000235:genetics; D003517:Cyclopentanes / Q000378:metabolism*; D060467:Disease Resistance / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism*; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D044224:Pseudomonas syringae / Q000472:pathogenicity / Q000502:physiology*; D015398:Signal Transduction; D014774:Virulence
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid; C586088:LecRK-I.9 protein, Arabidopsis; D011494:Protein Kinases; D005966:Glucuronidase
keywords: Arabidopsis; Pseudomonas; jasmonic acid; lectin; plant-pathogen interactions; receptor-like kinases