title: Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats.
abstract: Stem cell transplantation and low-energy shock-wave therapy (LESWT) have emerged as potential and effective treatment protocols for diabetic erectile dysfunction. During the tracking of transplanted stem cells in diabetic erectile dysfunction models, the number of visible stem cells was rather low and decreased quickly. LESWT could recruit endogenous stem cells to the cavernous body and improve the microenvironment in diabetic cavernous tissue. Thus, we deduced that LESWT might benefit transplanted stem cell survival and improve the effects of stem cell transplantation. In this research, 42 streptozotocin-induced diabetic rats were randomized into four groups: the diabetic group (n = 6), the LESWT group (n = 6), the bone marrow-derived mesenchymal stem cell (BMSC) transplantation group (n = 15), and the combination of LESWT and BMSC transplantation group (n = 15). One and three days after BMSC transplantation, three rats were randomly chosen to observe the survival numbers of BMSCs in the cavernous body. Four weeks after BMSC transplantation, the following parameters were assessed: the surviving number of transplanted BMSCs in the cavernous tissue, erectile function, real-time polymerase chain reaction, and penile immunohistochemical assessment. Our research found that LESWT favored the survival of transplanted BMSCs in the cavernous body, which might be related to increased stromal cell-derived factor-1 expression and the enhancement of angiogenesis in the diabetic cavernous tissue. The combination of LESWT and BMSC transplantation could improve the erectile function of diabetic erectile function rats more effectively than LESWT or BMSC transplantation performed alone.
authors.affiliation: Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China.|Department of Urology, Shawan People's Hospital, Panyu District, Guangzhou, China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Shan
authors.affiliation: Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Zhang
authors.affiliation: Shenzhen Hyde Medical Equipment Co., Ltd., Shenzhen, China.
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Chen
authors.affiliation: Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Feng-Zhi
authors.initials: FZ
authors.lastname: Chen
authors.affiliation: Department of Urology, Longjiang Hospital, Shunde District, Foshan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Jin-Tai
authors.initials: JT
authors.lastname: Luo
authors.affiliation: Laboratory Animals Center, Southern Medical University, Guangzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yue
authors.affiliation: Laboratory Animals Center, Southern Medical University, Guangzhou, China.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Lin
authors.affiliation: Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: An-Yang
authors.initials: AY
authors.lastname: Wei
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure; D054377:Chemokine CXCL12 / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000503:physiopathology*; D004195:Disease Models, Animal; D066026:Endothelial Progenitor Cells; D007172:Erectile Dysfunction / Q000209:etiology / Q000503:physiopathology*; D005434:Flow Cytometry; D007150:Immunohistochemistry; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010410:Penile Erection / Q000502:physiology*; D010413:Penis / Q000378:metabolism* / Q000473:pathology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000069453:Ultrasonic Waves; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D000199:Actins; D054377:Chemokine CXCL12; D052250:Nitric Oxide Synthase Type III; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D042461:Vascular Endothelial Growth Factor A; C575935:CXCL12 protein, rat; C498862:Acta2 protein, rat; C467498:vascular endothelial growth factor A, rat; C496316:Nos3 protein, rat