title: Interaction of stem cells with nano hydroxyapatite-fucoidan bionanocomposites for bone tissue regeneration.
abstract: The combination of bioceramics with biopolymers are playing major role in the construction of artificial bone. Hydroxyapatite (HA) has been extensively studied as a material in bone repair and replacement in last two decades. In the present study, we have prepared the hydroxyapatite-fucoidan (HA-Fucoidan) nanocomposites by in situ chemical method and biologically characterized them for bone graft substitute. Biological results inferred that mineralization effect of HA-F nanocomposites shows significant enhancement compared to HA in adipose derived stem cell (ADSC). It may be due to the addition of fucoidan in the nanocomposites. The important gene expression such as osteocalcin, osteopontin, collagen and runx-2 were checked using ADSC with HA and HA-fucoidan nanocomposites and the results show that the enhancements were found at 7th day. Furthermore, we have performed in vivo study of HA-fucoidan nanocomposites with rabbit model and a slight amount of bone formation was observed in HA-fucoidan nanocomposites. Herewith, we suggest that HA-fucoidan nanocomposites will be good biomaterials for bone repair/replacement in future.
authors.affiliation: Department of Orthopaedic Surgery and Medical Research Institute, Pusan National University Hospital, Busan 602-739, Republic of Korea.
authors.forename: Ahn
authors.initials: A
authors.lastname: Tae Young
authors.affiliation: Department of Orthopaedic Surgery and Medical Research Institute, Pusan National University Hospital, Busan 602-739, Republic of Korea.
authors.forename: Jeong Han
authors.initials: JH
authors.lastname: Kang
authors.affiliation: Department of Orthopaedic Surgery and Medical Research Institute, Pusan National University Hospital, Busan 602-739, Republic of Korea.
authors.forename: Dong Jun
authors.initials: DJ
authors.lastname: Kang
authors.affiliation: Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea; VPP, King Saud University, Riyadh 12372, Saudi Arabia.
authors.forename: Jayachandran
authors.initials: J
authors.lastname: Venkatesan
authors.affiliation: Department of Pathology, Medical College, Kosin University, Busan 602-739, Republic of Korea.
authors.forename: Hee Kyung
authors.initials: HK
authors.lastname: Chang
authors.affiliation: Clinical Research Facility, Medical Biotechnology Complex, Centre for Cellular and Molecular Biology, Hyderabad 500-007, India.
authors.forename: Ira
authors.initials: I
authors.lastname: Bhatnagar
authors.affiliation: Institute for Life Science of Seogo (ILSS), Kolmar Korea Co., Seoul 137-876, Republic of Korea.
authors.forename: Kwan-Young
authors.initials: KY
authors.lastname: Chang
authors.affiliation: Marine Techno Inc. 205 Knowledge Industry Center, Jeonnam Technopark, Suncheon, Jeonnam 58034, Republic of Korea.
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Hwang
authors.affiliation: Department of Prosthodontics, School of Dentistry, Lebanese University, Beirut 1107 2020, Lebanon.
authors.forename: Ziad
authors.initials: Z
authors.lastname: Salameh
authors.affiliation: Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: sknkim@pknu.ac.kr.
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Orthopaedic Surgery and Medical Research Institute, Pusan National University Hospital, Busan 602-739, Republic of Korea.
authors.forename: Hui Taek
authors.initials: HT
authors.lastname: Kim
authors.affiliation: Specialized Graduate School Science & Technology Convergence, Pukyong National University, Busan 608-739, Republic of Korea.
authors.forename: Dong Gyu
authors.initials: DG
authors.lastname: Kim
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry*; D001842:Bone and Bones / Q000502:physiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D006882:Hydroxyapatites / Q000737:chemistry*; D020007:Implants, Experimental; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000502:physiology; D053761:Nanocomposites / Q000737:chemistry*; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis; D011134:Polysaccharides / Q000737:chemistry*; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D006882:Hydroxyapatites; D011134:Polysaccharides; C007789:fucoidan
keywords: Biomaterials; Bone tissue engineering; Fucoidan; Hydroxyapatite