title: Effects of Pore Size on the Osteoconductivity and Mechanical Properties of Calcium Phosphate Cement in a Rabbit Model.
abstract: Calcium phosphate cement (CPC) porous scaffold is widely used as a suitable bone substitute to repair bone defect, but the optimal pore size is unclear yet. The current study aimed to evaluate the effect of different pore sizes on the processing of bone formation in repairing segmental bone defect of rabbits using CPC porous scaffolds. Three kinds of CPC porous scaffolds with 5 mm diameters and 12 mm length were prepared with the same porosity but different pore sizes (Group A: 200-300 µm, Group B: 300-450 µm, Group C: 450-600 µm, respectively). Twelve millimeter segmental bone defects were created in the middle of the radius bone and filled with different kinds of CPC cylindrical scaffolds. After 4, 12, and 24 weeks, alkaline phosphatase (ALP), histological assessment, and mechanical properties evaluation were performed in all three groups. After 4 weeks, ALP activity increased in all groups but was highest in Group A with smallest pore size. The new bone formation within the scaffolds was not obvious in all groups. After 12 weeks, the new bone formation within the scaffolds was obvious in each group and highest in Group A. At 24 weeks, no significant difference in new bone formation was observed among different groups. Besides the osteoconductive effect, Group A with smallest pore size also had the best mechanical properties in vivo at 12 weeks. We demonstrate that pore size has a significant effect on the osteoconductivity and mechanical properties of calcium phosphate cement porous scaffold in vivo. Small pore size favors the bone formation in the early stage and may be more suitable for repairing segmental bone defect in vivo.
authors.affiliation: Department of Orthopedics Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Yi-Nan
authors.initials: YN
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Fan
authors.affiliation: Department of Orthopedics Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Zhi-Quan
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: Department of Orthopedics Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Yan-Wu
authors.initials: YW
authors.lastname: Liu
authors.affiliation: Department of Orthopedics Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Yao-Ping
authors.initials: YP
authors.lastname: Wu
authors.affiliation: Department of Orthopedics Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001696:Biomechanical Phenomena; D001843:Bone Cements / Q000737:chemistry / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000737:chemistry / Q000627:therapeutic use*; D001842:Bone and Bones / Q000187:drug effects / Q000293:injuries; D002130:Calcium Phosphates / Q000737:chemistry / Q000627:therapeutic use*; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D016062:Porosity; D011817:Rabbits; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001843:Bone Cements; D018786:Bone Substitutes; D002130:Calcium Phosphates; C020243:calcium phosphate; D000469:Alkaline Phosphatase
keywords: Calcium phosphate cement; Mechanical properties; Osteoconductivity; Pore size; Segmental bone defect