title: Synthesis of Hollow Mesoporous Silica Nanorods with Controllable Aspect Ratios for Intracellular Triggered Drug Release in Cancer Cells.
abstract: Here, we have reported a straightforward and effective synthetic strategy for synthesis of aspect-ratios-controllable mesoporous silica nanorods with hollow structure (hMSR) and its application for transcription factor (TF)-responsive drug delivery intracellular. Templating by an acid-degradable nickel hydrazine nanorods (NHNT), we have first synthesized the hollow dense silica nanorods and then coated on a mesoporous silica layer. Subsequently, the dense silica layer was removed by the surface-protected etching method and the hollow structure of hMSR was finally formed. The aspect ratios of the hMSR can be conveniently controlled by regulating the aspect ratios of NHNT. Four different hMSR with aspect ratios of ca. 2.5, ca. 5.3, ca. 8.1, and ca. 9.0 has been obtained. It was demonstrated that the as-prepared hMSRs have good stability, high drug loading capacity, and fast cell uptake capability, which makes them to a potential nanocarrier for drug delivery. As the paradigm, hMSR with an aspect ratio of ca. 8.1 was then applied for TF-responsive intracellular anticancer drug controlled release by using a Ag(+)-stabilized molecular switch of triplex DNA (TDNA) as capping agents and probes for TFs recognition. In the presence of TF, the pores of hMSR can be unlocked by the TFs induced disassembly of TDNA, leading to the leakage of DOX. The research in vitro displayed that this system has a TFs-triggered DOX release, and the cytotoxicity in L02 normal cells was lower than that of HeLa cells. We hope that this developed hMSR-based system will promote the development of cancer therapy in related fields.
authors.affiliation: College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha 410082, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha 410082, China.
authors.forename: Dinggeng
authors.initials: D
authors.lastname: He
authors.affiliation: College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha 410082, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: He
authors.affiliation: College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha 410082, China.
authors.forename: Kemin
authors.initials: K
authors.lastname: Wang
authors.affiliation: College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha 410082, China.
authors.forename: Jinlu
authors.initials: J
authors.lastname: Tang
authors.affiliation: College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha 410082, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zou
authors.affiliation: College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha 410082, China.
authors.forename: Xing
authors.initials: X
authors.lastname: He
authors.affiliation: College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha 410082, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiong
authors.affiliation: College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha 410082, China.
authors.forename: Liling
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha 410082, China.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Shangguan
mesh_terms: D004317:Doxorubicin; D016503:Drug Delivery Systems; D065546:Drug Liberation; D006367:HeLa Cells; D006801:Humans; D043942:Nanotubes; D016062:Porosity; D012822:Silicon Dioxide
chemical_list: D012822:Silicon Dioxide; D004317:Doxorubicin
keywords: controllable aspect ratios; controlled release; hollow mesoporous silica nanorod; intracellular drug delivery; transcription factor (TFs)-responsive