title: Correlation between the level of microRNA expression in peripheral blood mononuclear cells and symptomatology in patients with generalized anxiety disorder.
abstract: This study investigated the correlation between the level of microRNA expression in peripheral blood mononuclear cells (PBMCs) and symptomatology in patients with generalized anxiety disorder (GAD). MicroRNA array was performed in peripheral blood mononuclear cells (PBMCs) obtained from GAD patients with gender, age, ethnicity-matched healthy controls. Then real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to verify the top 7 miRNAs with the highest fold-change values in 76 GAD patients and 39 healthy controls. It demonstrated that 5 miRNAs showed significantly differences in expression levels (P<0.01). These 5 GAD-associated miRNAs were finally selected into our study to analyze the association between the plasma level of miRNAs expression and symptomatology scores in Hamilton Anxiety Scale (HAMA). Results showed that the level of miR-4505 and miR-663 was negatively correlated with the total HAMA scores in GAD patients (r=0.2228, r=0.264 P<0.05). MiR-663 was selected into the regression equation of HAMA total scores and psychic anxiety symptomatology scores, and it could explain 5.3% of the HAMA total scores and 15.3% of the anxiety symptomatology scores. This study analyzed preliminarily possible circulating miRNAs expression changes in GAD patients, and the expression level of miR-663 highly correlated with psychic anxiety symptoms, further molecular mechanism of which needs to be explored.
authors.affiliation: Department of Psychiatry and Psychology, Second Military Medical University, Shanghai 200433, People's Republic of China; Department of Neurology, No.102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu 213003, People's Republic of China.
authors.forename: Sheng-Dong
authors.initials: SD
authors.lastname: Chen
authors.affiliation: Department of Psychiatry and Psychology, PingAn Health Cloud Company Ltd. of China, Shanghai 200030, People's Republic of China.
authors.forename: Xin-Yang
authors.initials: XY
authors.lastname: Sun
authors.affiliation: Department of Rehabilitation, No.102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu 213003, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Niu
authors.affiliation: Prevention and Treatment Center for Psychological Diseases, No.102 Hospital of Chinese People's Liberation Army, Changzhou 213003, Jiangsu, People's Republic of China.
authors.forename: Ling-Ming
authors.initials: LM
authors.lastname: Kong
authors.affiliation: Prevention and Treatment Center for Psychological Diseases, No.102 Hospital of Chinese People's Liberation Army, Changzhou 213003, Jiangsu, People's Republic of China.
authors.forename: Ming-Jun
authors.initials: MJ
authors.lastname: He
authors.affiliation: Cadre Ward, Chengdu Military General Hospital, Chengdu, Sichuan 610083, People's Republic of China.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Fan
authors.affiliation: GoPath Diagnostic Laboratory Co. Ltd, No.801, Changwuzhong Road, Changzhou, Jiangsu 213164, People's Republic of China.
authors.forename: Wan-Shuai
authors.initials: WS
authors.lastname: Li
authors.affiliation: Department of Laboratory, No.102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu 213003, People's Republic of China.
authors.forename: Ai-Fang
authors.initials: AF
authors.lastname: Zhong
authors.affiliation: Prevention and Treatment Center for Psychological Diseases, No.102 Hospital of Chinese People's Liberation Army, Changzhou 213003, Jiangsu, People's Republic of China. Electronic address: zly102@126.com.
authors.forename: Li-Yi
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: GoPath Diagnostic Laboratory Co. Ltd, No.801, Changwuzhong Road, Changzhou, Jiangsu 213164, People's Republic of China; GoPath Laboratories LLC, 1351 Barclay Blvd, Buffalo, Grove, IL 60089, United States. Electronic address: luzb88@hotmail.com.
authors.forename: Jim
authors.initials: J
authors.lastname: Lu
mesh_terms: D000328:Adult; D001008:Anxiety Disorders / Q000175:diagnosis* / Q000235:genetics* / Q000523:psychology; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D012016:Reference Values; D013223:Statistics as Topic
chemical_list: D035683:MicroRNAs