title: [Influence of ET-1 and ETA receptor blocker (BQ123) on the level of TNF-α in the brain rat].
abstract: Endothelin 1 (ET-1) in addition to the vasoconstriction, also has mitogenic, proinflammatory and proagregation activities. The mediators of inflammatory responses are cytokines, including special role attributed to tumor necrosis factor (TNF-α).
  
  
  OBJECTIVE
  The aim of this study was to evaluate the effect of ET-1 and its receptor blocker (BQ123) on the level of TNF-α in the brain rat.
  
  
  METHODS
  Experiments were performed on four groups of Wistar-Kyoto rats. Animals were divided into four groups of 8 rats. Group I - control was administered into the tail vein solution of 0.9 % NaCl. Group II - saline followed by ET-1 (3 μg/kg b.w.). Group III - saline followed by BQ123 (1 mg/kg b.w.). Group IV (BQ123/ET-1) - BQ123 (1 mg/kg b.w.) administered 30 min before ET-1 (3 μg/kg b.w.).
  
  
  RESULTS
  Administration of ET-1 at doses of 3 μg/kg b.w. resulted in a statistically significant increase in TNF-α concentrations in brain homogenates compared to the control group (p<0.01). Administration of the ET(A) receptor blocker - BQ123 (1mg/kg b.w.) 30 min before administration of ET-1 significantly decreased in TNF-α concentrations in brain homogenates (p <0.01).
  
  
  CONCLUSIONS
  ET-1 is significantly increased in TNF-α levels in brain homogenates, while BQ123 given 30 min before administration of ET-1 caused a significant decrease in TNF-α levels, suggesting that its anti-inflammatory activity.
authors.affiliation: Medical University of Łódź, Poland: Chair of Experimental and Clinical Physiology, Department of Circulation Physiology.
authors.forename: Anna
authors.initials: A
authors.lastname: Gorąca
authors.affiliation: Medical University of Łódź, Poland: Chair of Applied Pharmacy, Department of Pharmacy.
authors.forename: Beata
authors.initials: B
authors.lastname: Skibska
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects*; D001923:Brain Chemistry; D065128:Endothelin Receptor Antagonists / Q000494:pharmacology*; D019332:Endothelin-1 / Q000037:antagonists & inhibitors*; D008297:Male; D010456:Peptides, Cyclic / Q000494:pharmacology*; D051381:Rats; D011921:Rats, Inbred WKY; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D065128:Endothelin Receptor Antagonists; D019332:Endothelin-1; D010456:Peptides, Cyclic; D014409:Tumor Necrosis Factor-alpha; C072247:cyclo(Trp-Asp-Pro-Val-Leu)
keywords: ETA receptor blocker (BQ123); TNF-α; brain; endothelin 1