title: Glaucoma related Proteomic Alterations in Human Retina Samples.
abstract: Glaucoma related proteomic changes have been documented in cell and animal models. However, proteomic studies investigating on human retina samples are still rare. In the present work, retina samples of glaucoma and non-glaucoma control donors have been examined by a state-of-the-art mass spectrometry (MS) workflow to uncover glaucoma related proteomic changes. More than 600 proteins could be identified with high confidence (FDR < 1%) in human retina samples. Distinct proteomic changes have been observed in 10% of proteins encircling mitochondrial and nucleus species. Numerous proteins showed a significant glaucoma related level change (p < 0.05) or distinct tendency of alteration (p < 0.1). Candidates were documented to be involved in cellular development, stress and cell death. Increase of stress related proteins and decrease of new glaucoma related candidates, ADP/ATP translocase 3 (ANT3), PC4 and SRFS1-interacting protein 1 (DFS70) and methyl-CpG-binding protein 2 (MeCp2) could be documented by MS. Moreover, candidates could be validated by Accurate Inclusion Mass Screening (AIMS) and immunostaining and supported for the retinal ganglion cell layer (GCL) by laser capture microdissection (LCM) in porcine and human eye cryosections. The workflow allowed a detailed view into the human retina proteome highlighting new molecular players ANT3, DFS70 and MeCp2 associated to glaucoma.
authors.affiliation: Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Funke
authors.affiliation: Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Natarajan
authors.initials: N
authors.lastname: Perumal
authors.affiliation: Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Beck
authors.affiliation: Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Silke
authors.initials: S
authors.lastname: Gabel-Scheurich
authors.affiliation: Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Schmelter
authors.affiliation: Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Teister
authors.affiliation: Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Gerbig
authors.affiliation: Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.|Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa, USA.
authors.forename: Oliver W
authors.initials: OW
authors.lastname: Gramlich
authors.affiliation: Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Pfeiffer
authors.affiliation: Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Franz H
authors.initials: FH
authors.lastname: Grus
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D005901:Glaucoma / Q000378:metabolism*; D006801:Humans; D061346:Laser Capture Microdissection; D008297:Male; D008970:Molecular Weight; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012160:Retina / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000378:metabolism; D034421:Sus scrofa
chemical_list: D015415:Biomarkers; D020543:Proteome