title: Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples.
abstract: Culture-based detection of nontuberculous Mycobacteria (NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust.
authors.affiliation: SAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, South Australia, Australia.
authors.forename: Gianny P
authors.initials: GP
authors.lastname: Scoleri
authors.affiliation: SAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, South Australia, Australia.
authors.forename: Jocelyn M
authors.initials: JM
authors.lastname: Choo
authors.affiliation: SAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, South Australia, Australia.
authors.forename: Lex E X
authors.initials: LE
authors.lastname: Leong
authors.affiliation: Department of Respiratory and Medicine, WCH, and Paediatrics and Reproductive Health, School of Medicine, University of Adelaide, Australia.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Goddard
authors.affiliation: Microbiology Infectious Diseases Directorate, SA Pathology, Adelaide, South Australia.
authors.forename: Lisa
authors.initials: L
authors.lastname: Shephard
authors.affiliation: Immunity, Infection and Inflammation Program, Mater Research Institute, University of Queensland, Translational Research Institute, Wooloongabba, QLD, Australia Department of Respiratory Medicine, Mater Health Services, South Brisbane, QLD, Australia.
authors.forename: Lucy D
authors.initials: LD
authors.lastname: Burr
authors.affiliation: Microbiology Infectious Diseases Directorate, SA Pathology, Adelaide, South Australia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Bastian
authors.affiliation: Gallipoli Medical Research Centre, Greenslopes Private Hospital and University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Thomson
authors.affiliation: SAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, South Australia, Australia geraint.rogers@sahmri.com.
authors.forename: Geraint B
authors.initials: GB
authors.lastname: Rogers
mesh_terms: D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis*; D009170:Nontuberculous Mycobacteria / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity