title: Glyco-centric lectin magnetic bead array (LeMBA) - proteomics dataset of human serum samples from healthy, Barrett׳s esophagus and esophageal adenocarcinoma individuals.
abstract: This data article describes serum glycoprotein biomarker discovery and qualification datasets generated using lectin magnetic bead array (LeMBA) - mass spectrometry techniques, "Serum glycoprotein biomarker discovery and qualification pipeline reveals novel diagnostic biomarker candidates for esophageal adenocarcinoma" [1]. Serum samples collected from healthy, metaplastic Barrett׳s esophagus (BE) and esophageal adenocarcinoma (EAC) individuals were profiled for glycoprotein subsets via differential lectin binding. The biomarker discovery proteomics dataset consisting of 20 individual lectin pull-downs for 29 serum samples with a spiked-in internal standard chicken ovalbumin protein has been deposited in the PRIDE partner repository of the ProteomeXchange Consortium with the data set identifier PRIDE: PXD002442. Annotated MS/MS spectra for the peptide identifications can be viewed using MS-Viewer (〈http://prospector2.ucsf.edu/prospector/cgi-bin/msform.cgi?form=msviewer〉) using search key "jn7qafftux". The qualification dataset contained 6-lectin pulldown-coupled multiple reaction monitoring-mass spectrometry (MRM-MS) data for 41 protein candidates, from 60 serum samples. This dataset is available as a supplemental files with the original publication [1].
authors.affiliation: The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, Queensland, Australia.
authors.forename: Alok K
authors.initials: AK
authors.lastname: Shah
authors.affiliation: The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, Queensland, Australia.
authors.forename: Kim-Anh
authors.initials: KA
authors.lastname: Lê Cao
authors.affiliation: The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, Queensland, Australia; School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
authors.forename: Eunju
authors.initials: E
authors.lastname: Choi
authors.affiliation: School of Information and Communication Technology, Griffith University, Brisbane, Queensland, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Chen
authors.affiliation: The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, Queensland, Australia.
authors.forename: Benoît
authors.initials: B
authors.lastname: Gautier
authors.affiliation: QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors.forename: Derek
authors.initials: D
authors.lastname: Nancarrow
authors.affiliation: QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors.forename: David C
authors.initials: DC
authors.lastname: Whiteman
authors.affiliation: Mass Spectrometry Facility, Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA.
authors.forename: Peter R
authors.initials: PR
authors.lastname: Baker
authors.affiliation: Proteomics Platform, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
authors.forename: Karl R
authors.initials: KR
authors.lastname: Clauser
authors.affiliation: Mass Spectrometry Facility, Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Chalkley
authors.affiliation: The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, Queensland, Australia.
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Saunders
authors.affiliation: School of Medicine, The University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Barbour
authors.affiliation: Ochsner Health System, Gastroenterology, New Orleans, LA, USA.
authors.forename: Virendra
authors.initials: V
authors.lastname: Joshi
authors.affiliation: The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, Queensland, Australia.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Hill
keywords: Barrett׳s esophagus; Biomarker; Esophageal adenocarcinoma; Glycoprotein; Proteomics