title: Modulation of renal ischemia/reperfusion in rats by a combination of ischemic preconditioning and adipose-derived mesenchymal stem cells (ADMSCs).
abstract: The present study investigated the effects of combination of ischemic preconditioning (Ipre) and adipose-derived mesenchymal stem cells (ADMSCs) on renal ischemia-reperfusion (I-R) injury in rats. 90 male Sprague Dawley rats were divided into 5 equal groups; sham operated, control (45 min left renal ischemia), Ipre group as control group with 3 cycles of Ipre just before renal ischemia, ADMSCs-treated group (as control with ADMSCs 10(6) cells in 0.1 mL via penile vein 60 min before ischemia time), and Ipre + ADMSCs group as ADMCs group with 3 cycles of Ipre. Ipre and ADMSCs groups showed significant decrease in serum creatinine and blood urea nitrogen (BUN) and caspase-3 and CD45 expression in kidney and significant increase in HIF-1α, SDF-1α, CD31, and Ki67 expressions in kidney compared with the control group (p < 0.05). Moreover, the Ipre + ADMSCs group showed significant decrease in serum BUN and caspase-3 and CD45 expression in kidney with significant increase in HIF-1α, SDF-1α, CD31, and Ki67 expression in kidney compared with the Ipre and ADMCs groups (p < 0.05). We concluded that Ipre potentiates the renoprotective effect of ADMSCs against renal I/R injury probably by upregulation of HIF-1α, SDF-1α, CD31, and Ki67 and downregulation of caspase-3 and CD45.
authors.affiliation: a Physiology Department, Faculty of Medicine, Mansoura University, Mansoura, 35516, Egypt.
authors.forename: Abdelaziz M
authors.initials: AM
authors.lastname: Hussein
authors.affiliation: b Urology and Nephrology Center, Mansoura University, Mansoura, 35516, Egypt.
authors.forename: Nashwa
authors.initials: N
authors.lastname: Barakat
authors.affiliation: b Urology and Nephrology Center, Mansoura University, Mansoura, 35516, Egypt.
authors.forename: Amira
authors.initials: A
authors.lastname: Awadalla
authors.affiliation: b Urology and Nephrology Center, Mansoura University, Mansoura, 35516, Egypt.
authors.forename: Mahmoud M
authors.initials: MM
authors.lastname: Gabr
authors.affiliation: b Urology and Nephrology Center, Mansoura University, Mansoura, 35516, Egypt.
authors.forename: Sherry
authors.initials: S
authors.lastname: Khater
authors.affiliation: b Urology and Nephrology Center, Mansoura University, Mansoura, 35516, Egypt.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Harraz
authors.affiliation: b Urology and Nephrology Center, Mansoura University, Mansoura, 35516, Egypt.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Shokeir
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001806:Blood Urea Nitrogen; D053148:Caspase 3 / Q000096:biosynthesis; D054377:Chemokine CXCL12 / Q000096:biosynthesis; D003404:Creatinine / Q000097:blood; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis; D019194:Ischemic Preconditioning; D019394:Ki-67 Antigen / Q000096:biosynthesis; D007668:Kidney / Q000378:metabolism* / Q000473:pathology*; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D051381:Rats; D015427:Reperfusion Injury / Q000097:blood / Q000378:metabolism* / Q000473:pathology*
chemical_list: C575935:CXCL12 protein, rat; D054377:Chemokine CXCL12; C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D019394:Ki-67 Antigen; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D003404:Creatinine; D017493:Leukocyte Common Antigens; C516771:Ptprc protein, rat; D053148:Caspase 3
keywords: ADMC; ADMSC; CD31; CD45; HIF-1α; Ki67; SDF-1α; ischemic preconditioning; ischémie/reperfusion rénale; préconditionnement ischémique; renal ischemia/reperfusion