title: [Intermolecular Interactions between Cytisine and Bovine Serum Albumin A Synchronous Fluorescence Spectroscopic Analysis and Molecular Docking Research].
abstract: Cytisine (Cy) is one of the alkaloids that exist naturally in the plant genera Laburnum of the family Fabaceae. With strong bioactivities, Cy is commercialized for smoking cessation for years. In this work, the study of intermolecular interactions between Cy and bovine serum albumin (BSA) was performed by applying fluorescence spectroscopic methods under simulated physiological conditions. The mechanism of fluorescence quenching of BSA by Cy was also studied. Parameters such as bathing temperature, time and solution pH were investigated to optimize the fluorescence quenching. The binding type, binding ratio and binding constant between BSA and Cy were calculated by using the Stem-Volmer equation. Experimental results indicated that Cy can quench the fluorescent emission of BSA statically by forming a 1 : 1 type non-covalent complex and the binding constant is 5.6 x 10(3) L x mol(-1). Synchronous fluorescence spectral research shows Cy may affect the fluorescence emission of Trp residues of BSA. Furthermore, molecular docking is utilized to model the complex and probe the plausible quenching mechanism. It can be noted that the hydrogen bindings and hydrophobic interactions between Cy and BSA change the micro-environment of Trp213, which leads to the fluorescence quenching of BSA.
authors.forename: Yu-hang
authors.initials: YH
authors.lastname: Wu
authors.forename: Zhong-bao
authors.initials: ZB
authors.lastname: Han
authors.forename: Jia-ze
authors.initials: JZ
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Liu
authors.forename: Shi-gang
authors.initials: SG
authors.lastname: Xin
authors.forename: Zhan
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D001392:Azocines / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D062105:Molecular Docking Simulation; D011807:Quinolizines / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D000093843:Quinolizidine Alkaloids
chemical_list: D000470:Alkaloids; D001392:Azocines; D011807:Quinolizines; D012710:Serum Albumin, Bovine; C004712:cytisine; D000093843:Quinolizidine Alkaloids