title: Characterization of Transgenic Silkworm Yielded Biomaterials with Calcium-Binding Activity.
abstract: Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG)6ASEYDYDDDSDDDDEWD]2 from shell nacreous matrix protein. It can be produced as fibers by transgenic silkworm. The Ca-binding activity and mineralization of the transgenic silk fibroin were examined in vitro. The results showed that this transgenic silk fibroin had relatively higher Ca-binding activity than unmodified silk fibroin. The increased Ca-binding activity could promote the usage of silk fibroin as a biomaterial in the pharmaceutical industry. This study shows the possibility of using silk fibroin as a mineralization accelerating medical material by generating genetically modified transgenic silkworm.
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Mingying
authors.initials: M
authors.lastname: Yang
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Lupeng
authors.initials: L
authors.lastname: Ye
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Gong
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Qiujie
authors.initials: Q
authors.lastname: Qian
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Shuai
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Zhengying
authors.initials: Z
authors.lastname: You
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Yuyin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Boxiong
authors.initials: B
authors.lastname: Zhong
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001672:Biocompatible Materials; D012831:Bombyx; D002118:Calcium / Q000378:metabolism*; D005349:Fibroins / Q000378:metabolism*; D047011:Silk / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D005349:Fibroins; D002118:Calcium