title: 2'-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA.
abstract: Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2'-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.
authors.affiliation: Department of Chemistry, Chemistry Research Laboratory, University of Oxford , Oxford, OX1 3TA, U.K.
authors.forename: Marius M
authors.initials: MM
authors.lastname: Haugland
authors.affiliation: Department of Chemistry, Chemistry Research Laboratory, University of Oxford , Oxford, OX1 3TA, U.K.|Chemistry Branch, Faculty of Petroleum and Mining Engineering, Suez University , Suez 43721, Egypt.
authors.forename: Afaf H
authors.initials: AH
authors.lastname: El-Sagheer
authors.affiliation: Department of Chemistry, Chemistry Research Laboratory, University of Oxford , Oxford, OX1 3TA, U.K.
authors.forename: Rachel J
authors.initials: RJ
authors.lastname: Porter
authors.affiliation: Departamento de Química Orgánica, Universidad de Salamanca , Plaza de los Caídos 1-5, 37008 Salamanca, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Peña
authors.affiliation: Department of Chemistry, Chemistry Research Laboratory, University of Oxford , Oxford, OX1 3TA, U.K.
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
authors.affiliation: Department of Chemistry, Chemistry Research Laboratory, University of Oxford , Oxford, OX1 3TA, U.K.
authors.forename: Edward A
authors.initials: EA
authors.lastname: Anderson
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews , North Haugh, St Andrews, KY16 9SS, U.K.
authors.forename: Janet E
authors.initials: JE
authors.lastname: Lovett
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001483:Base Sequence; D059371:DNA, B-Form / Q000737:chemistry* / Q000235:genetics*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D000480:Alkynes; D059371:DNA, B-Form; D009711:Nucleotides; D013113:Spin Labels