title: Bioreducible Gene Delivery Vector Capable of Self-Scavenging the Intracellular-Generated ROS Exhibiting High Gene Transfection.
abstract: Cationic polymer vectors have received increasing attention for gene delivery in biotechnology over the past 2 decades, but few polymer vectors were used in clinical applications due to their low gene transfection efficacy. One of the major reasons is that the conventional cationic polymers can induce the increasing of intracellular reactive oxygen species (ROS) concentration and oxidative stress, which reduces the gene transfection efficacy. Here, we create a novel class of thioether dendron-branched polymer conjugate and self-assemble this conjugate into bioreducible cationic nanomicelles with disulfide bond connecting the thioether core to the cationic shell. The obtained nanomicelles have a unique ROS self-scavenging ability, thereby dramatically improving gene transfection efficacy.
authors.affiliation: Key Laboratory of Soft Matter Chemistry, Chinese Academy of Science, Department of Polymer Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
authors.forename: Long-Hai
authors.initials: LH
authors.lastname: Wang
authors.affiliation: Key Laboratory of Soft Matter Chemistry, Chinese Academy of Science, Department of Polymer Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: De-Cheng
authors.initials: DC
authors.lastname: Wu
authors.affiliation: Key Laboratory of Soft Matter Chemistry, Chinese Academy of Science, Department of Polymer Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
authors.forename: Ye-Zi
authors.initials: YZ
authors.lastname: You
mesh_terms: D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000737:chemistry* / Q000378:metabolism*; D011108:Polymers; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014162:Transfection / Q000379:methods* / Q000592:standards
chemical_list: D011108:Polymers; D017382:Reactive Oxygen Species
keywords: bioreducible nanomicelles; gene delivery; reactive oxygen species; self-assemble; self-scavenge