title: TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses.
abstract: As a benthic filter feeder of estuaries, the immune system of oysters provides one of the best models for studying the genetic and molecular basis of the innate immune pathway in marine invertebrates and examining the influence of environmental factors on the immune system. Here, the molecular function of molluscan TANK-binding kinase-1 (TBK1) (which we named CgTBK1) was studied in the Pacific oyster, Crassostrea gigas. Compared with known TBK1 proteins in other model organisms, CgTBK1 contains a conserved S-TKc domain and a coiled coil domain at the N- and C-terminals but lacks an important ubiquitin domain. Quantitative real-time PCR analysis revealed that the expression level of CgTBK1 was ubiquitous in all selected tissues, with highest expression in the gills. CgTBK1 expression was significantly upregulated in response to infections with Vibrio alginolyticus, ostreid herpesvirus 1 (OsHV-1 reference strain and μvar), and polyinosinic:polycytidylic acid sodium salt, suggesting its broad function in immune response. Subcellular localization showed the presence of CgTBK1 in the cytoplasm of HeLa cells, suggesting its potential function as the signal transducer between the receptor and transcription factor. We further demonstrated that CgTBK1 interacted with CgSTING in HEK293T cells, providing evidence that CgTBK1 could be activated by direct binding to CgSTING. In summary, we characterized the TBK1 gene in C. gigas and demonstrated its role in the innate immune response to pathogen infections.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, China; University of Chinese Academy of Sciences, Beijing 100039, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266071, China; National & Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao, Shandong 266071, China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Tang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266071, China; National & Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao, Shandong 266071, China.
authors.forename: Baoyu
authors.initials: B
authors.lastname: Huang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266071, China; National & Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao, Shandong 266071, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, China; Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266071, China; National & Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao, Shandong 266071, China. Electronic address: lili@qdio.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266071, China; National & Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao, Shandong 266071, China. Electronic address: gfzhang@qdio.ac.cn.
authors.forename: Guofan
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D049896:Crassostrea / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004267:DNA Viruses / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D010802:Phylogeny; D011070:Poly I-C / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution; D044149:Vibrio alginolyticus / Q000502:physiology*
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D004247:DNA; D017346:Protein Serine-Threonine Kinases; D011070:Poly I-C
keywords: Crassostrea gigas; Innate immunity; Pacific oyster; STING; TBK1