title: First evidence of bioflocculant from Shinella albus with flocculation activity on harvesting of Chlorella vulgaris biomass.
abstract: Bioflocculant from Shinella albus xn-1 could be used to harvest energy-producing microalga Chlorella vulgaris biomass for the first time. In this study, we investigated the flocculation activity and mode of strain xn-1, the characteristics of bioflocculant, the effect of flocculation conditions and optimized the flocculation efficiency. The results indicated that strain xn-1 exhibited flocculation activity through secreting bioflocculant; the bioflocculant with high thermal stability, pH stability and low molecular weight was proved to be not protein and polysaccharide, and flocculation active component was confirmed to contain triple bond and cumulated double bonds; algal pH, temperature and metal ions showed great impacts on the flocculation efficiency of bioflocculant; the maximum flocculation activity of bioflocculant reached 85.65% after the response surface optimization. According to the results, the bioflocculant from S. albus xn-1 could be a good potential in applications for high-efficiency harvesting of microalgae.
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang 453007, China; State Key Laboratory of Marine Environmental Science, Xiamen University, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Jiang
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Tianling
authors.initials: T
authors.lastname: Zheng
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang 453007, China. Electronic address: liyi@htu.edu.cn.
authors.forename: Hailei
authors.initials: H
authors.lastname: Wang
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D048408:Chlorella vulgaris / Q000737:chemistry / Q000302:isolation & purification; D005427:Flocculation; D058086:Microalgae / Q000737:chemistry / Q000302:isolation & purification; D012230:Rhizobiaceae / Q000737:chemistry / Q000378:metabolism
keywords: Bioflocculant; Biomass harvest; Chlorella vulgaris; Flocculation activity optimization; Shinella albus