title: A New Generation of Smart Amine Donors for Transaminase-Mediated Biotransformations.
abstract: The application of ω-transaminase biocatalysts for the synthesis of optically pure chiral amines presents a number of challenges, including difficulties associated with displacing the challenging reaction equilibria. Herein, we report a highly effective approach using low equivalents of the new diamine donor, cadaverine, which enables high conversions of challenging substrates to the corresponding chiral amines in excellent ee. This approach paves the way for the design of self-sufficient fermentation processes combining transaminase biotransformations with existing strategies for cadaverine production by decarboxylation of endogenous lysine.
authors.affiliation: School of Chemistry, University of Nottingham, University Park, NG7 2RD, Nottingham, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Gomm
authors.affiliation: School of Chemistry, University of Nottingham, University Park, NG7 2RD, Nottingham, UK.
authors.forename: William
authors.initials: W
authors.lastname: Lewis
authors.affiliation: Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Green
authors.affiliation: School of Chemistry, University of Nottingham, University Park, NG7 2RD, Nottingham, UK. elaine.oreilly@nottingham.ac.uk.
authors.forename: Elaine
authors.initials: E
authors.lastname: O'Reilly
mesh_terms: D000588:Amines / Q000138:chemical synthesis* / Q000737:chemistry; D055162:Biocatalysis; D001711:Biotransformation; D000637:Transaminases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000588:Amines; D000637:Transaminases
keywords: asymmetric catalysis; biocatalysis; chiral amines; diamines; transaminases