title: Shotgun Quantitative Proteomic Analysis of Proteins Responding to Drought Stress in Brassica rapa L. (Inbred Line "Chiifu").
abstract: Through a comparative shotgun quantitative proteomics analysis in Brassica rapa (inbred line Chiifu), total of 3,009 nonredundant proteins were identified with a false discovery rate of 0.01 in 3-week-old plants subjected to dehydration treatment for 0, 24, and 48 h, plants subjected to drought stress. Ribulose-bisphosphate carboxylases, chlorophyll a/b-binding protein, and light harvesting complex in photosystem II were highly abundant proteins in the leaves and accounted for 9%, 2%, and 4%, respectively, of the total identified proteins. Comparative analysis of the treatments enabled detection of 440 differentially expressed proteins during dehydration. The results of clustering analysis, gene ontology (GO) enrichment analysis, and analysis of composite expression profiles of functional categories for the differentially expressed proteins indicated that drought stress reduced the levels of proteins associated with photosynthesis and increased the levels of proteins involved in catabolic processes and stress responses. We observed enhanced expression of many proteins involved in osmotic stress responses and proteins with antioxidant activities. Based on previously reported molecular functions, we propose that the following five differentially expressed proteins could provide target genes for engineering drought resistance in plants: annexin, phospholipase D delta, sDNA-binding transcriptional regulator, auxin-responsive GH3 family protein, and TRAF-like family protein.
authors.affiliation: Department of Plant Bioscience, Pusan National University, Milyang 627-706, Republic of Korea.
authors.forename: Soon-Wook
authors.initials: SW
authors.lastname: Kwon
authors.affiliation: Department of Applied Bioscience, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Mijeong
authors.identifier: 0000-0003-0736-0183
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Applied Bioscience, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hijin
authors.identifier: 0000-0001-8156-9474
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Applied Bioscience, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Joohyun
authors.identifier: 0000-0003-4539-8342
authors.initials: J
authors.lastname: Lee