title: Strategies of isoprenoids production in engineered bacteria.
abstract: Isoprenoids are the largest family of natural products, over 40 000 compounds have been described, which have been widely used in various fields. Currently, the isoprenoid products are mainly produced by natural extraction or chemical synthesis, however, limited yield and high cost is far behind the increasing need. Most bacteria synthesize the precursors of isoprenoids through the methylerythritol 4-phosphate pathway, microbial synthesis of isoprenoids by fermentation becomes more attractive mainly in terms of environmental concern and renewable resources. In this review, the strategies of isoprenoid production in bacteria by synthetic biology are discussed. Introducing foreign genes associated with desired products made it possible to produce isoprenoids in bacteria. Furthermore, the yield of isoprenoids is increased by the strategies of overexpression of native or foreign genes, introducing heterologous mevalonate pathway, balancing of the precursors and inactivating the competing pathway, these methods were used separately or simultaneously.
authors.affiliation: College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, Chengdu, China. wanggg@cib.ac.cn.|Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China. wanggg@cib.ac.cn.
authors.forename: G
authors.initials: G
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways; D004896:Erythritol / Q000031:analogs & derivatives / Q000378:metabolism; D005285:Fermentation; D005818:Genetic Engineering; D008798:Mevalonic Acid / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: C114232:2-C-methylerythritol 4-phosphate; D013403:Sugar Phosphates; D013729:Terpenes; D004896:Erythritol; D008798:Mevalonic Acid
keywords: biosynthesis; isoprenoids; methylerythritol 4-phosphate pathway; mevalonate pathway; microbial synthesis