title: Strategies for enhancing resveratrol production and the expression of pathway enzymes.
abstract: Trans-resveratrol (trans-3,5,4'-trihydroxystilbene) is one of the most promising stilbenes, a type of natural phenol that is produced naturally by some plant species in response to stress. Resveratrol exhibits multiple bioactivities and is used in the agriculture, medical, food, and cosmetic industries due to its antitumor, anti-inflammatory, cardioprotective, and antioxidant properties. Due to the increasing demand, an active area of investigation is the use of plant cell culture and metabolic engineering techniques to produce large quantities of active resveratrol. However, most recent studies have focused on the efficiency of synthesizing resveratrol in vitro, but have not investigated the contributions of the transcriptional activities of the genes encoding the related enzymes in the biosynthesis pathway. This article reviews recently developed methods for the biosynthesis of resveratrol and comprehensively reviews the current state of knowledge of the function of the key pathway enzymes in resveratrol synthesis. Approaches for enhancing resveratrol production, such as introducing non-pathway genes and co-localizing enzymes are described in detail.
authors.affiliation: Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, Shaanxi Province, 710072, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, Shaanxi Province, 710072, China.
authors.forename: Dongyan
authors.initials: D
authors.lastname: Shao
authors.affiliation: Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, Shaanxi Province, 710072, China. sjlshi2004@nwpu.edu.cn.
authors.forename: Junling
authors.initials: J
authors.lastname: Shi
authors.affiliation: Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, Shaanxi Province, 710072, China.
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, Shaanxi Province, 710072, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, Shaanxi Province, 710072, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Jin
mesh_terms: D000893:Anti-Inflammatory Agents / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D018929:Cell Culture Techniques; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D059828:Plant Cells / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D000077185:Resveratrol; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013267:Stilbenes / Q000138:chemical synthesis* / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D013267:Stilbenes; D000077185:Resveratrol
keywords: Biosynthesis pathway; Key enzymes; Metabolic engineering; Phenylpropanoid pathway; Resveratrol