title: The Calponin Family Member CHDP-1 Interacts with Rac/CED-10 to Promote Cell Protrusions.
abstract: Eukaryotic cells extend a variety of surface protrusions to direct cell motility. Formation of protrusions is mediated by coordinated actions between the plasma membrane and the underlying actin cytoskeleton. Here, we found that the single calponin homology (CH) domain-containing protein CHDP-1 induces the formation of cell protrusions in C. elegans. CHDP-1 is anchored to the cortex through its amphipathic helix. CHDP-1 associates through its CH domain with the small GTPase Rac1/CED-10, which is a key regulator of the actin cytoskeleton. CHDP-1 preferentially binds to the GTP-bound active form of the CED-10 protein and preserves the membrane localization of GTP-CED-10. Hence, by coupling membrane expansion to Rac1-mediated actin dynamics, CHDP-1 promotes the formation of cellular protrusions in vivo.
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Guan
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Xuehua
authors.initials: X
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.|CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai, China.
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.|CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Ding
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D022081:Cell Surface Extensions / Q000378:metabolism*; D005786:Gene Expression Regulation; D008840:Microfilament Proteins / Q000378:metabolism*; D008957:Models, Genetic; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010641:Phenotype; D011485:Protein Binding; D017422:Sequence Analysis, DNA; D019076:Transgenes; D020744:rac GTP-Binding Proteins / Q000378:metabolism*; D000096985:Calponins
chemical_list: D000199:Actins; D029742:Caenorhabditis elegans Proteins; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D020559:Monomeric GTP-Binding Proteins; D020744:rac GTP-Binding Proteins; D000096985:Calponins; C407268:CED-10 protein, C elegans; C000616312:CHDP-1 protein, C elegans