title: Effects of Oleanane-Type Triterpene Saponins from the Leaves of Eleutherococcus senticosus in an Axonal Outgrowth Assay.
abstract: An aqueous extract of Eleutherococcus senticosus leaves exerted a beneficial effect in restoring the neurite outgrowth from Aβ25-35-induced degeneration using an axonal density assay. Subsequent bioassay-guided fractionation afforded seven new oleanane-type triterpene saponins, ezoukoginosides A-G (1-7), along with nine known analogues. The structures of 1-7 were elucidated through chemical and spectroscopic approaches, and their effects on restoring the neurite outgrowth from Aβ25-35-induced degeneration were investigated. The results revealed that hydrophilic oleanane-type saponins substituted with a free carboxylic acid, hydroxy, or formyl group in the aglycone, especially when the oxidation occurred at C-29, not only restrained Aβ25-35-induced degeneration but also restored axonal outgrowth significantly. Compounds 2 (-COOH at C-29) and 3 (-CH2OH at C-29) showed the most potent bioactivity among the isolates.
authors.forename: Yue-Wei
authors.initials: YW
authors.lastname: Ge
authors.forename: Chihiro
authors.initials: C
authors.lastname: Tohda
authors.forename: Shu
authors.initials: S
authors.lastname: Zhu
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: He
authors.affiliation: Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health and Nutrition , Ibaraki 567-0085, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Katsuko
authors.initials: K
authors.lastname: Komatsu
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D028001:Eleutherococcus / Q000737:chemistry*; D005260:Female; D007564:Japan; D051379:Mice; D015394:Molecular Structure; D000071446:Neuronal Outgrowth / Q000187:drug effects*; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D011247:Pregnancy; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D010936:Plant Extracts; D012503:Saponins; C074636:amyloid beta-protein (25-35); C413246:oleanane; D009828:Oleanolic Acid