title: Expression of metalloproteinases (MMP-2 and MMP-9) in basal-cell carcinoma.
abstract: The aim of this study was to compare the expressions of mRNA for metalloproteinases (MMP-2 and MMP-9) and type IV collagen in two different histological types of basal-cell carcinoma (BCCs; nodular and infiltrative) and in normal tissues from the tumor interface. The study included biopsy specimens of the skin involved with BCC and normal skin adjacent the lesion. The expressions of mRNA for MMP-2, MMP-9 and type IV collagen were determined by means of RT-PCR (Reverse transcription polymerase chain reaction). The level of type IV collagen mRNA in nodular and infiltrative BCCs turned out to be significantly lower, and the expressions of MMP-2 and MMP-9 mRNA significantly higher than in normal tissues adjacent to these tumors. The expression of mRNA for MMP-9 but not for MMP-2 was significantly higher in infiltrative BCCs than in the nodular BCCs. In turn, normal tissues adjacent to nodular BCCs showed significantly higher levels of mRNA for MMP-2 and significantly lower levels of type IV collagen mRNA than the normal tissues from the interface of infiltrative BCCs. The findings suggest that MMP-2 and MMP-9 could be used as prognostic factors of BCCs.
authors.affiliation: Department of Health and Medical Sciences, Andrzej Frycz Modrzewski Krakow University, 1 G. Herlinga-Grudzińskiego St, 30-705, Krakow, Poland. anna.gozdzialska@gmail.com.
authors.forename: Anna
authors.initials: A
authors.lastname: Goździalska
authors.affiliation: Department of Dermatology, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Wojas-Pelc
authors.affiliation: Department of Health and Medical Sciences, Andrzej Frycz Modrzewski Krakow University, 1 G. Herlinga-Grudzińskiego St, 30-705, Krakow, Poland.
authors.forename: Jagoda
authors.initials: J
authors.lastname: Drąg
authors.affiliation: Department of Dermatology, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Brzewski
authors.affiliation: Department of Health and Medical Sciences, Andrzej Frycz Modrzewski Krakow University, 1 G. Herlinga-Grudzińskiego St, 30-705, Krakow, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Jaśkiewicz
authors.affiliation: Department of Dermatology, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Pastuszczak
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002280:Basal Cell Carcinoma / Q000235:genetics* / Q000473:pathology; D024141:Collagen Type IV / Q000235:genetics; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D024141:Collagen Type IV; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Basal-cell carcinoma; Invasiveness; Matrix metalloproteinases; Molecular diagnosis; Resection margin; Type IV collagen