title: Tandem mass spectral libraries of peptides and their roles in proteomics research.
abstract: Proteomics is a rapidly maturing field aimed at the high-throughput identification and quantification of all proteins in a biological system. The cornerstone of proteomic technology is tandem mass spectrometry of peptides resulting from the digestion of protein mixtures. The fragmentation pattern of each peptide ion is captured in its tandem mass spectrum, which enables its identification and acts as a fingerprint for the peptide. Spectral libraries are simply searchable collections of these fingerprints, which have taken on an increasingly prominent role in proteomic data analysis. This review describes the historical development of spectral libraries in proteomics, details the computational procedures behind library building and searching, surveys the current applications of spectral libraries, and discusses the outstanding challenges. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 36:634-648, 2017.
authors.affiliation: Department of Biology, Institute of Molecular Systems Biology, Eidgenössische Technische Hochschule (ETH) Zurich, Zurich, Switzerland.|Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
authors.forename: Wenguang
authors.initials: W
authors.lastname: Shao
authors.affiliation: Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.|Department of Chemical and Biomolecular Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
authors.forename: Henry
authors.initials: H
authors.lastname: Lam
mesh_terms: D000465:Algorithms; D000818:Animals; D057225:Data Mining; D030562:Databases, Protein; D049673:History, 20th Century; D049674:History, 21st Century; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D012984:Software; D053719:Tandem Mass Spectrometry / Q000266:history / Q000379:methods* / Q000706:statistics & numerical data; D014584:User-Computer Interface
chemical_list: D010455:Peptides
keywords: proteomics; spectral library; tandem mass spectrometry