title: [Interaction of two tumor suppressors: Phosphatase CTDSPL and Rb protein].
abstract: Earlier we established that CTDSPL gene encoding small carboxy-terminal domain serine phosphatase can be considered a classical tumor suppressor gene. Besides, transfection of tumor cell line MCF-7 with CTDSPL led to the content decrease of inactive phosphorylated form of another tumor suppressor, retinoblastoma protein (Rb), and subsequently to cell cycle arrest at the G1/S boundary. This result implied that small phosphatase CTDSPL is able to specifically dephosphorylate and activate Rb protein. In order to add some fuel to this hypothesis, in the present work we studied the interaction of two tumor suppressors CTDSPL and Rb in vitro. GST pool-down assay revealed that CTDSPL is able to precipitate Rb protein from MCF-7 cell extracts, while surface plasmon resonance technique showed that interaction of the two proteins is direct. Results of this study reassert that phosphatase CTDSPL and Rb could be involved in the common mechanism of cell cycle regulation.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.|abeniaminov@mail.ru.
authors.forename: A D
authors.initials: AD
authors.lastname: Beniaminov
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: G S
authors.initials: GS
authors.lastname: Krasnov
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Dmitriev
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: G A
authors.initials: GA
authors.lastname: Puzanov
authors.affiliation: Lashkaryov Institute of Semiconductor Physics, National Academy of Sciences of Ukraine, Kiev, 03028, Ukraine.
authors.forename: B A
authors.initials: BA
authors.lastname: Snopok
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Senchenko
authors.affiliation: Department of Molecular Oncogenetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, 03680, Ukraine.
authors.forename: V I
authors.initials: VI
authors.lastname: Kashuba
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D047468:Immunoprecipitation; D061986:MCF-7 Cells; D027361:Organic Anion Transporters / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016160:Retinoblastoma Protein / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D027361:Organic Anion Transporters; D011993:Recombinant Fusion Proteins; D016160:Retinoblastoma Protein; D025521:Tumor Suppressor Proteins; C486483:CTDSPL protein, human; C487009:SLCO6A1 protein, human
keywords: CTDSPL; Rb; protein interaction; tumor suppressor gene