title: Proteomic analysis of drug-resistant Mycobacterium tuberculosis by one-dimensional gel electrophoresis and charge chromatography.
abstract: Multidrug-resistant tuberculosis (MDR-TB) is a form of TB caused by Mycobacterium tuberculosis (M. tuberculosis) that do not respond to, at least, isoniazid and rifampicin, the two most powerful, first-line (or standard) anti-TB drugs. Novel intervention strategies for eliminating this disease were based on finding proteins that can be used for designing new drugs or new and reliable kits for diagnosis. The aim of this study was to compare the protein profiles of MDR-TB with sensitive isolates. Proteomic analysis of M. tuberculosis MDR-TB and sensitive isolates was obtained with ion exchange chromatography coupled with MALDI-TOF-TOF (matrix-assisted laser desorption/ionization) in order to identify individual proteins that have different expression in MDR-TB to be used as a drug target or diagnostic marker for designing valuable TB vaccines or TB rapid tests. We identified eight proteins in MDR-TB isolates, and analyses showed that these proteins are absent in M. tuberculosis-sensitive isolates: (Rv2140c, Rv0009, Rv1932, Rv0251c, Rv2558, Rv1284, Rv3699 and MMP major membrane proteins). These data will provide valuable clues in further investigation for suitable TB rapid tests or drug targets against drug-resistant and sensitive M. tuberculosis isolates.
authors.affiliation: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran.|Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Shamsi
authors.initials: S
authors.lastname: Yari
authors.affiliation: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran.|Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Hadizadeh Tasbiti
authors.affiliation: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran.|Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Ghanei
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.affiliation: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran.|Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Fateh
authors.affiliation: Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Mahdian
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Yari
authors.affiliation: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran. tbchemistry@gmail.com.
authors.forename: Ahmadreza
authors.initials: A
authors.lastname: Bahrmand
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002845:Chromatography; D024881:Drug Resistance, Bacterial; D004586:Electrophoresis; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins
keywords: Ion exchange chromatography (IEC); MDR-TB; Mass spectrometry; Sensitive TB; TB proteomics