title: Anaerobic and sequential aerobic production of high-titer ethanol and single cell protein from NaOH-pretreated corn stover by a genome shuffling-modified Saccharomyces cerevisiae strain.
abstract: In this study, a Saccharomyces cerevisiae recombinant strain 14 was constructed through genome shuffling method by transferring the whole genomic DNA of Candida intermedia strain 23 into a thermo-tolerant S. cerevisiae strain. The recombinant strain 14 combined the good natures of both parent strains that efficiently produced ethanol from glucose and single cell protein from xylose with 54.6% crude protein and all essential amino acids except cysteine at 35°C. Importantly, the recombinant strain 14 produced 64.07g/L ethanol from 25%(w/v) NaOH-pretreated and washed corn stover with the ethanol yield of 0.26g/g total stover by fed-batch simultaneous saccharification and fermentation and produced 66.50g/L dry cell mass subsequently from the residual hydrolysate and ethanol. Therefore, this study represents a feasible method to comprehensively utilize hexose and pentose in lignocellulosic materials.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Xueliang
authors.initials: X
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Juncong
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Chunlan
authors.initials: C
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Hu
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; Hubei Collaborative Innovation Center for Industrial Fermentation, Wuhan 430068, PR China.
authors.forename: Shumiao
authors.initials: S
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; Hubei Collaborative Innovation Center for Industrial Fermentation, Wuhan 430068, PR China.
authors.forename: Yunxiang
authors.initials: Y
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; Hubei Collaborative Innovation Center for Industrial Fermentation, Wuhan 430068, PR China; Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Chengdu 610041, Sichuan, PR China. Electronic address: nanp@mail.hzau.edu.cn.
authors.forename: Nan
authors.initials: N
authors.lastname: Peng
mesh_terms: D000601:Amino Acids, Essential / Q000032:analysis; D056804:Biofuels; D032621:DNA Shuffling; D004044:Dietary Proteins / Q000032:analysis / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*; D003313:Zea mays
chemical_list: D000601:Amino Acids, Essential; D056804:Biofuels; D004044:Dietary Proteins; C012551:single cell proteins; D000431:Ethanol; D014994:Xylose; D005947:Glucose
keywords: Corn stover; Ethanol fermentation; Genome shuffling; Simultaneous saccharification and fermentation; Single cell protein