title: The quantification of blood-brain barrier disruption using dynamic contrast-enhanced magnetic resonance imaging in aging rhesus monkeys with spontaneous type 2 diabetes mellitus.
abstract: Microvascular lesions of the body are one of the most serious complications that can affect patients with type 2 diabetes mellitus. The blood-brain barrier (BBB) is a highly selective permeable barrier around the microvessels of the brain. This study investigated BBB disruption in diabetic rhesus monkeys using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). Multi-slice DCE-MRI was used to quantify BBB permeability. Five diabetic monkeys and six control monkeys underwent magnetic resonance brain imaging in 3 Tesla MRI system. Regions of the frontal cortex, the temporal cortex, the basal ganglia, the thalamus, and the hippocampus in the two groups were selected as regions of interest to calculate the value of the transport coefficient Ktransusing the extended Tofts model. Permeability in the diabetic monkeys was significantly increased as compared with permeability in the normal control monkeys. Histopathologically, zonula occludens protein-1 decreased, immunoglobulin G leaked out of the blood, and nuclear factor E2-related factor translocated from the cytoplasm to the nuclei. It is likely that diabetes contributed to the increased BBB permeability.
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Ziqian
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Sichuan Primed Bio-Tech Group Co., Ltd., Chengdu, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zeng
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Ruzhi
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Sichuan Primed Bio-Tech Group Co., Ltd., Chengdu, China.
authors.forename: Zunyuan
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Sichuan Primed Bio-Tech Group Co., Ltd., Chengdu, China.
authors.forename: Zunwei
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Li
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Yushu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Wang
authors.affiliation: Sichuan Primed Bio-Tech Group Co., Ltd., Chengdu, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gong
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology and Institute of Neurological Disease, Translation Neuroscience Center, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Tinghua
authors.initials: T
authors.lastname: Wang
authors.affiliation: Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu, China. Electronic address: gaofabao@yahoo.com.
authors.forename: Fabao
authors.initials: F
authors.lastname: Gao
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000000981:diagnostic imaging* / Q000473:pathology*; D002199:Capillary Permeability; D003287:Contrast Media; D003924:Diabetes Mellitus, Type 2 / Q000473:pathology*; D005260:Female; D007089:Image Enhancement / Q000379:methods; D008253:Macaca mulatta; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male
chemical_list: D003287:Contrast Media
keywords: Blood-brain barrier (BBB); Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI); Extended Tofts model; K(trans); Type 2 diabetes mellitus (T2DM)