title: MicroRNA-135b inhibits odontoblast-like differentiation of human dental pulp cells by regulating Smad5 and Smad4.
abstract: OBJECTIVE
  To investigate the function of miRNAs in odontoblast-like differentiation of human dental pulp cells (hDPCs).
  
  
  METHODS
  Integrated comparative miRNA microarray profiling was used to determine the differential miRNAs expression in odontoblast-like differentiation of hDPCs. The abundance of microRNA-135b (miR-135b) was measured by quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) and in situ hybridization (ISH). Bioinformatic analyses combined with luciferase assays were utilized to identify the targets interacting with miR-135b. Overexpression of miR-135b was performed to investigate the role and mechanism in odontoblast-like differentiation of hDPCs. Statistical analysis was performed by one-way analysis of variance (anova) or Student's t-test.
  
  
  RESULTS
  Thirty-six differentially expressed microRNAs in odontoblast-like differentiation of hDPCs were identified. MiR-135b expression was significantly downregulated during hDPCs differentiation (P < 0.05). In addition, miR-135b was able to bind to the 3'-UTR of the Smad5 and Smad4 and repressed these two genes expression (P < 0.05). Furthermore, overexpression of miR-135b suppressed odontoblast-like differentiation of hDPCs and attenuated the expression of Smad5 and Smad4 (P < 0.05).
  
  
  CONCLUSIONS
  These observations indicated a potential role of miR-135b in mediating odontoblast-like differentiation of hDPCs and inhibition of miR-135b might be a promising therapeutic way to facilitate dentine tissue engineering.
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Chen
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
authors.forename: R F
authors.initials: RF
authors.lastname: Wang
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
authors.forename: W
authors.initials: W
authors.lastname: Qin
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
authors.forename: S H
authors.initials: SH
authors.lastname: Huang
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Lin
authors.affiliation: Department of Stomatology, Hainan General Hospital, Haikou, Hainan, China.
authors.forename: Y G
authors.initials: YG
authors.lastname: Tian
mesh_terms: D000293:Adolescent; D015153:Blotting, Western; D002454:Cell Differentiation; D002478:Cells, Cultured; D019295:Computational Biology / Q000379:methods; D003782:Dental Pulp / Q000166:cytology*; D015536:Down-Regulation; D006801:Humans; D017403:In Situ Hybridization; D008156:Luciferases; D035683:MicroRNAs / Q000378:metabolism*; D008964:Molar, Third; D009804:Odontoblasts / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D051901:Smad4 Protein / Q000378:metabolism*; D051902:Smad5 Protein / Q000378:metabolism*; D055815:Young Adult
chemical_list: D035683:MicroRNAs; C497796:SMAD4 protein, human; C497797:SMAD5 protein, human; D051901:Smad4 Protein; D051902:Smad5 Protein; D008156:Luciferases
keywords: MiR-135b; Smad4; Smad5; cell differentiation; odontoblasts