title: A Highly Selective and Sensitive Fluorescence Detection Method of Glyphosate Based on an Immune Reaction Strategy of Carbon Dot Labeled Antibody and Antigen Magnetic Beads.
abstract: A sensitive fluorescence detection method for glyphosate (GLY) was established based on immune reaction. First, carbon dot labeled antibodies (lgG-CDs) which were able to specifically identify glyphosate were prepared with the environmentally friendly carbon dots (CDs) and glyphosate antibody (lgG). lgG-CDs could be used to in situ visualize the distribution of glyphosate in plant tissues. In order to eliminate the effects of excess lgG-CDs on the determination of GLY, antigen magnetic beads Fe3O4-GLY based on magnetic nanoparticles Fe3O4 and glyphosate were constructed and utilized to couple with the excess lgG-CDs. After magnetic separation to remove antigen magnetic beads, there was a linear relationship between the fluorescence intensity of lgG-CDs and the logarithmic concentration of glyphosate in the range of 0.01-80 μg/mL with a detection limit of 8 ng/mL. The method was used for the detection of glyphosate in Pearl River water, tea, and soil samples with satisfactory recovery ratio between 87.4% and 103.7%.
authors.affiliation: School of Chemistry and Environment, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou , Guangzhou, Guangdong 510006, China.
authors.forename: Duo
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Chemistry and Environment, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou , Guangzhou, Guangdong 510006, China.
authors.forename: Bixia
authors.initials: B
authors.lastname: Lin
authors.affiliation: School of Chemistry and Environment, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou , Guangzhou, Guangdong 510006, China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: School of Chemistry and Environment, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou , Guangzhou, Guangdong 510006, China.
authors.forename: Manli
authors.initials: M
authors.lastname: Guo
authors.affiliation: School of Chemistry and Environment, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou , Guangzhou, Guangdong 510006, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods; D002244:Carbon / Q000737:chemistry*; D005453:Fluorescence; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis; D060328:Magnetic Phenomena; D053758:Nanoparticles / Q000737:chemistry; D045483:Rivers / Q000737:chemistry; D012710:Serum Albumin, Bovine; D012987:Soil / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013662:Tea / Q000737:chemistry; D000097797:Glyphosate
chemical_list: D000906:Antibodies; D002244:Carbon; D005998:Glycine; D012710:Serum Albumin, Bovine; D012987:Soil; D013662:Tea; D000097797:Glyphosate
keywords: carbon dots; fluorescence immunoassay; glyphosate; magnetic beads; quantitative determination