title: Characterization data on the topical carrier DDC642.
abstract: This article contains original data, figures and methods used in the characterization of the liposomal carrier 'DDC642' for topical applications, described in "An elastic liposomal formulation for RNAi-based topical treatment of skin disorders: proof-of-concept in the treatment of psoriasis" (Desmet et al., 2016) [1]. Several elastic liposomal formulations have been evaluated for their ability to encapsulate and deliver RNA interference (RNAi) molecules to cultured primary skin cells. The efficiency and effectiveness of these liposomes were compared to that of our previously characterized liposomes, the 'SECosomes' (SEC) (Geusens et al., 2010) [2]. After selection of a potential superior carrier, based on encapsulation and transfection efficiency data (Desmet et al., 2016) [1], the selected DDC642 liposomes were characterized more in-depth. Herein, a detailed characterization of the DDC642 liposome and RNAi-loaded lipoplexes is given, including the matching protocols.
authors.affiliation: Department of Dermatology, Ghent University, Ghent, Belgium.
authors.forename: Eline
authors.initials: E
authors.lastname: Desmet
authors.affiliation: Department of Dermatology, Ghent University, Ghent, Belgium.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Bracke
authors.affiliation: Department of Pharmaceutics, Ghent University, Ghent, Belgium; Center of Nano and Biophotonics, Ghent University, Ghent, Belgium.
authors.forename: Katrien
authors.initials: K
authors.lastname: Forier
authors.affiliation: Department of Pharmaceutical Analysis, Ghent University, Ghent, Belgium.
authors.forename: Lien
authors.initials: L
authors.lastname: Taevernier
authors.affiliation: Electron microscopy, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.
authors.forename: Marc C A
authors.initials: MC
authors.lastname: Stuart
authors.affiliation: Department of Pharmaceutical Analysis, Ghent University, Ghent, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: De Spiegeleer
authors.affiliation: Department of Pharmaceutics, Ghent University, Ghent, Belgium.
authors.forename: Koen
authors.initials: K
authors.lastname: Raemdonck
authors.affiliation: Department of Dermatology, Ghent University, Ghent, Belgium.
authors.forename: Mireille
authors.initials: M
authors.lastname: Van Gele
authors.affiliation: Department of Dermatology, Ghent University, Ghent, Belgium.
authors.forename: Jo
authors.initials: J
authors.lastname: Lambert
keywords: Gene therapy; Lipid-based nanoparticle; Liposome; RNA interference; Topical drug delivery