title: An ultrasensitive electrochemical genosensor for Brucella based on palladium nanoparticles.
abstract: Palladium nanoparticles were potentiostatically electrodeposited on a gold surface at a highly negative potential. The nanostructure, as a transducer, was utilized to immobilize a Brucella-specific probe and the process of immobilization and hybridization was detected by electrochemical methods. The proposed method for detection of the complementary sequence and a non-complementary sequence was applied. The fabricated genosensor was evaluated for the assay of the bacteria in the cultured and human samples with and without PCR. The genosensor could detect the complementary sequence with a sensitivity of 0.02 μA dm(3) mol(-1), a linear concentration range of 1.0 × 10(-12) to 1.0 × 10(-19) mol dm(-3), and a detection limit of 2.7 × 10(-20) mol dm(-3).
authors.affiliation: Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Rahi
authors.affiliation: Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: sattarahmady@yahoo.com.
authors.forename: N
authors.initials: N
authors.lastname: Sattarahmady
authors.affiliation: Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: hheli7@yahoo.com.
authors.forename: H
authors.initials: H
authors.lastname: Heli
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D002002:Brucella / Q000145:classification / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D010165:Palladium
keywords: Brucella; DNA biosensor; Genosensor; Nanoparticles; Palladium