title: Estimation of the diversity between DNA calorimetric profiles, differential melting curves and corresponding melting temperatures.
abstract: The Poland-Fixman-Freire formalism was adapted for modeling of calorimetric DNA melting profiles, and applied to plasmid pBR 322 and long random sequences. We studied the influence of the difference (HGC -HAT ) between the helix-coil transition enthalpies of AT and GC base pairs on the calorimetric melting profile and on normalized calorimetric melting profile. A strong alteration of DNA calorimetrical profile with HGC -HAT was demonstrated. In contrast, there is a relatively slight change in the normalized profiles and in corresponding ordinary (optical) normalized differential melting curves (DMCs). For fixed HGC -HAT , the average relative deviation (S) between DMC and normalized calorimetric profile, and the difference between their melting temperatures (Tcal -Tm ) are weakly dependent on peculiarities of the multipeak fine structure of DMCs. At the same time, both the deviation S and difference (Tcal -Tm ) enlarge with the temperature melting range of the helix-coil transition. It is shown that the local deviation between DMC and normalized calorimetric profile increases in regions of narrow peaks distant from the melting temperature.
authors.affiliation: Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
authors.forename: Chun-Ling
authors.initials: CL
authors.lastname: Chang
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, 220141, Belarus.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Fridman
authors.affiliation: Department of Physics, Yerevan State University, Yerevan, 0025, Armenia.
authors.forename: Inessa E
authors.initials: IE
authors.lastname: Grigoryan
authors.affiliation: Department of Bioorganic Chemistry, Belarusian State Medical University, Minsk, 220116, Belarus.
authors.forename: Elena N
authors.initials: EN
authors.lastname: Galyuk
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
authors.forename: Oleg N
authors.initials: ON
authors.lastname: Murashko
authors.affiliation: Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.|National Center for Theoretical Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan.|Business School, University of Shanghai for Science and Technology, Shanghai 200093, China.
authors.forename: Chin-Kun
authors.initials: CK
authors.lastname: Hu
authors.affiliation: Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.|Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, 220141, Belarus.
authors.forename: Dmitri Y
authors.initials: DY
authors.lastname: Lando
mesh_terms: D002153:Calorimetry, Indirect / Q000379:methods; D004247:DNA / Q000737:chemistry*; D009691:Nucleic Acid Denaturation
chemical_list: D004247:DNA
keywords: DNA differential melting curve; DNA helix-coil transition; Differential scanning calorimetry; complex DNA systems; high-resolution calorimetric melting profiles