title: How do toxic metals affect harmful cyanobacteria? An integrative study with a toxigenic strain of Microcystis aeruginosa exposed to nickel stress.
abstract: Nickel (Ni) is an essential metal for some organisms, but also a common toxic pollutant released into the water. Toxicity of Ni has not been completely established for cyanobacteria; for this reason, we evaluated the effect of sub-inhibitory Ni concentrations on a toxigenic strain of Microcystis aeruginosa and on microcystins production. Population growth, photosynthetic pigments concentration, biomarkers, including antioxidant enzymes (catalase [CAT], glutathione peroxidase [GPx], and superoxide dismutase [SOD]), as well as macromolecules (proteins, carbohydrates and lipids) were quantified; SEM and TEM observations were also performed. Population growth was affected starting at 3µgL(-1), and at 24µgL(-1) growth was completely inhibited; the 96-h Ni(2+) IC50 was 3.7µgL(-1). Ni exposure increased pigments concentration, augmented all the macromolecules, and increased activities of CAT and GPx; alterations on the internal cell structure were also observed. The integrated biomarker response revealed that Ni(2+) augmented the antioxidant response and the macromolecules content. Ni stress also increased microcystins production. M. aeruginosa was affected by Ni at very low concentrations, even lower than those established as safe limit to protect aquatic biota. Aside from the toxic effects produced in this cyanobacterium, stimulation to produce toxins could potentiate the environmental risks associated with water pollution and eutrophication.
authors.affiliation: Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Laboratorio de Hidrobiología Experimental, Carpio y Plan de Ayala S/N, Col. Santo Tomás, Mexico, D.F. 11340, Mexico.
authors.forename: Erika Berenice
authors.initials: EB
authors.lastname: Martínez-Ruiz
authors.affiliation: Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Laboratorio de Hidrobiología Experimental, Carpio y Plan de Ayala S/N, Col. Santo Tomás, Mexico, D.F. 11340, Mexico. Electronic address: ferjeronimo@hotmail.com.
authors.forename: Fernando
authors.initials: F
authors.lastname: Martínez-Jerónimo
mesh_terms: D001426:Bacterial Proteins / Q000187:drug effects; D015415:Biomarkers / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002374:Catalase / Q000187:drug effects; D005068:Eutrophication / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000187:drug effects; D008055:Lipids / Q000032:analysis; D052998:Microcystins / Q000187:drug effects; D046931:Microcystis / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009532:Nickel / Q000633:toxicity*; D010788:Photosynthesis / Q000187:drug effects; D013482:Superoxide Dismutase / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D001426:Bacterial Proteins; D015415:Biomarkers; D002241:Carbohydrates; D008055:Lipids; D052998:Microcystins; D014874:Water Pollutants, Chemical; C078588:microcystin; D009532:Nickel; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Aquatic ecotoxicology; Harmful algal blooms; Integrated biomarker response; Oxidative stress; Phytoplankton; Toxic metals