title: Near-Infrared-Responsive Peptide that Targets Collagen Fibrils to Induce Cytotoxicity.
abstract: A novel conjugate, PHG10 dye, was synthesized using a collagen peptide and a near-infrared (NIR)-responsive dye to achieve targeted cytotoxicity. The collagen peptide motif, -(Pro-Hyp-Gly)10 - (PHG10), was incorporated for targeting collagen fibrils that are excessively produced by activated fibroblasts around tumor cells. PHG10 dye was purified by HPLC and identified by MALDI-MS. The phototoxicity and cytotoxicity of PHG10 dye were examined using human glioma cells (HGCs). Fluorescent images indicated that PHG10 dye preferably assembled to collagen-coated HGCs compared with noncoated HGCs. Under irradiation with NIR light, effective cytotoxicity was observed on collagen-coated HGCs within 20 min. Because phototoxicity and cytotoxicity are dependent on the assembled amount of PHG10 dye, the targeting of collagen fibrils by the collagen peptide motif PHG10 is assured.
authors.affiliation: Graduate School of Health Sciences, Komazawa University, Tokyo, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Honda
authors.affiliation: Graduate School of Engineering, Tohoku Institute of Technology, Sendai, Japan.
authors.forename: Aoi
authors.initials: A
authors.lastname: Odawara
authors.affiliation: Graduate School of Engineering, Tohoku Institute of Technology, Sendai, Japan.
authors.forename: Ikuro
authors.initials: I
authors.lastname: Suzuki
authors.affiliation: Graduate School of Health Sciences, Komazawa University, Tokyo, Japan.
authors.forename: Morio
authors.initials: M
authors.lastname: Shimada
authors.affiliation: Graduate School of Health Sciences, Komazawa University, Tokyo, Japan.
authors.forename: Kohki
authors.initials: K
authors.lastname: Yoshikawa
authors.affiliation: Graduate School of Health Sciences, Komazawa University, Tokyo, Japan. okada@komazawa-u.ac.jp.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Okada
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects*; D003094:Collagen / Q000187:drug effects*; D004396:Coloring Agents; D003603:Cytotoxins / Q000494:pharmacology; D016503:Drug Delivery Systems; D005910:Glioma / Q000188:drug therapy / Q000532:radiotherapy; D006801:Humans; D007259:Infrared Rays; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology* / Q000528:radiation effects*
chemical_list: D004396:Coloring Agents; D003603:Cytotoxins; D010455:Peptides; C083881:(prolyl-hydroxylprolyl-glycine)10; D003094:Collagen