title: Evaluation of Vitamin C Supplementation on Kidney Function and Vascular Reactivity Following Renal Ischemic Injury in Mice.
abstract: OBJECTIVE
  Renal ischemia/reperfusion injury (IRI) is a very common clinical event and usually leads to ischemic acute renal failure (ARF). In the present study, we investigated the protective role of vitamin C in renal function and renal arterial relaxation following ischemic injury.
  
  
  METHODS
  IRI model in mice was used. Various biochemical parameters including nitric oxide (NO), reduced glutathione (GSH), total reactive oxygen species (ROS) level and superoxide dismutase (SOD) were examined. Doppler was used to investigate renal arterial resistance. The isolated renal arterial rings served for hypoxia/reoxygenation analysis. Acetylcholine (ACh) and sodium nitroprusside (SNP)-induced relaxations of isolated renal arterial rings were exerted.
  
  
  RESULTS
  Vitamin C supplementation preserved kidney morphology and renal function following IRI. It was shown that pretreatment with vitamin C for mice subjected to IRI significantly elevated renal NO and GSH levels after reperfusion. Meanwhile, vitamin C administration decreased resistance index of renal artery and ameliorated oxidative stress secondary to IRI. The total ROS level in renal artery was decreased whereas the renal arterial SOD expression was increased by vitamin C supplementation following IRI. Pretreatment with vitamin C significantly potentiated the ACh and SNP-induced relaxations in both control and hypoxic renal arterial rings.
  
  
  CONCLUSIONS
  Vitamin C protects kidney function and renal arterial reactivity against IRI. The protective role of vitamin C is linked to ROS, SOD, GSH and NO levels in renal ischemic injury.
authors.affiliation: Pediatric Heart Center, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Yao-Bin
authors.initials: YB
authors.lastname: Zhu
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yan-Bo
authors.initials: YB
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D019587:Dietary Supplements; D005978:Glutathione / Q000032:analysis; D007668:Kidney / Q000098:blood supply / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000032:analysis; D017382:Reactive Oxygen Species / Q000032:analysis; D012077:Renal Artery / Q000187:drug effects* / Q000503:physiopathology; D015427:Reperfusion Injury / Q000188:drug therapy / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000032:analysis; D059289:Vascular Stiffness
chemical_list: D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid