title: AgNO3dependant modulation of glucose mediated respiration kinetics in Escherichia coli at different pH and temperature.
abstract: The inhibitory role of AgNO3on glucose-mediated respiration in Escherichia coli has been investigated as a function of pH and temperature using Clark-type electrode, environmental scanning electron microscopy, and computational tools. In the given concentration of bacterial suspension (1 × 108CFU/ml), E. coli showed an increasing nonlinear trend of tetra-phasic respiration between 1-133 μM glucose concentration within 20 min. The glucose concentrations above 133 μM did not result any linear increment in respiration but rather showed a partial inhibition at higher glucose concentrations (266-1066 μM). In the presence of glucose, AgNO3caused a concentration-dependent (47-1960 μM) inhibition of the respiration rate within 4 min of its addition. The respiration rate was the highest at pH 7-8 and then was decreased on either side of this pH range. The inhibitory action of AgNO3upon bacterial respiration was the highest at 37 °C. The observations of the respiration data were well supported by the altered bacterial morphology as observed in electron microscopic study. Docking study indicated the AgNO3binding to different amino acids of all respiratory complex enzymes in E. coli and thereby explaining its interference with the respiratory chain. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Laboratory of Fundamentals of Pharmaceutics, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Bandar Puncak Alam, Selangor, Malaysia.
authors.forename: Radin Nur
authors.initials: RN
authors.lastname: Afiqah
authors.affiliation: Department of Zoology, NC Govt. College, Jajpur, Odisha, India.
authors.forename: Biswaranjan
authors.initials: B
authors.lastname: Paital
authors.affiliation: Institute of Life Sciences, Bhubaneswar, Odisha, India.|ICAR-NBAIM, Kushmaur, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Laboratory of Fundamentals of Pharmaceutics, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Bandar Puncak Alam, Selangor, Malaysia.|Pharmaceutical and Life Sciences Core, Universiti Teknologi MARA (UiTM), Shah Alam, Selangor Darul Ehsan, Malaysia.
authors.forename: Abu Bakar Abdul
authors.initials: AB
authors.lastname: Majeed
authors.affiliation: Laboratory of Fundamentals of Pharmaceutics, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Bandar Puncak Alam, Selangor, Malaysia. minaketan@puncakalam.uitm.edu.my.|Pharmaceutical and Life Sciences Core, Universiti Teknologi MARA (UiTM), Shah Alam, Selangor Darul Ehsan, Malaysia. minaketan@puncakalam.uitm.edu.my.
authors.forename: Minaketan
authors.initials: M
authors.lastname: Tripathy
mesh_terms: D001665:Binding Sites; D004579:Electron Transport / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D062105:Molecular Docking Simulation; D012835:Silver Nitrate / Q000494:pharmacology*; D013696:Temperature
chemical_list: D029968:Escherichia coli Proteins; D012835:Silver Nitrate; D005947:Glucose
keywords: E. coli; respiration; respiratory enzymes; silver nitrate