title: The effect of amino acids on lipid production and nutrient removal by Rhodotorula glutinis cultivation in starch wastewater.
abstract: In this paper, the components of amino acids in mixed starch wastewater (corn steep water/corn gluten water=1/3, v/v) were analyzed by GC-MS. Effects of amino acids on lipid production by Rhodotorula glutinis and COD removal were studied. The results showed that mixed starch wastewater contained 9 kinds of amino acids and these amino acids significantly improved the biomass (13.63g/L), lipid yield (2.48g/L) and COD removal compared to the basic medium (6.23g/L and 1.56g/L). In a 5L fermentor containing mixed starch wastewater as substrate to culture R. glutinis, the maximum biomass, lipid content and lipid yield reached 26.38g/L, 28.90% and 7.62g/L, with the associated removal rates of COD, TN and TP reaching 77.41%, 69.12% and 73.85%, respectively. The results revealed a promising approach for lipid production with using amino acids present in starch wastewater as an alternative nitrogen source.
authors.affiliation: Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Liu
authors.affiliation: Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: zhangxu@mail.buct.edu.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D000596:Amino Acids / Q000494:pharmacology*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism; D012248:Rhodotorula / Q000254:growth & development / Q000378:metabolism*; D013213:Starch / Q000378:metabolism; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology*; D003313:Zea mays / Q000378:metabolism
chemical_list: D000596:Amino Acids; D008055:Lipids; D009584:Nitrogen; D013213:Starch; D062065:Wastewater
keywords: Amino acid; Lipid production; Nitrogen source; Nutrients removal; Starch wastewater