title: OsPAP10c, a novel secreted acid phosphatase in rice, plays an important role in the utilization of external organic phosphorus.
abstract: Under phosphate (Pi ) starvation, plants increase the secretion of purple acid phosphatases (PAPs) into the rhizosphere to scavenge organic phosphorus (P) for plant use. To date, only a few members of the PAP family have been characterized in crops. In this study, we identified a novel secreted PAP in rice, OsPAP10c, and investigated its role in the utilization of external organic P. OsPAP10c belongs to a monocotyledon-specific subclass of Ia group PAPs and is specifically expressed in the epidermis/exodermis cell layers of roots. Both the transcript and protein levels of OsPAP10c are strongly induced by Pi starvation. OsPAP10c overexpression increased acid phosphatase (APase) activity by more than 10-fold in the culture media and almost fivefold in both roots and leaves under Pi -sufficient and Pi -deficient conditions. This increase in APase activity further improved the plant utilization efficiency of external organic P. Moreover, several APase isoforms corresponding to OsPAP10c were identified using in-gel activity assays. Under field conditions with three different Pi supply levels, OsPAP10c-overexpressing plants had significantly higher tiller numbers and shorter plant heights. This study indicates that OsPAP10c encodes a novel secreted APase that plays an important role in the utilization of external organic P in rice.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Linghong
authors.initials: L
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Wenmin
authors.initials: W
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Gao
authors.affiliation: Australian Research Council Centre of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide, PMB1, Glen Osmond, South Australia, 5064, Australia.
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Tyerman
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China. huixia@zju.edu.cn.
authors.forename: Huixia
authors.initials: H
authors.lastname: Shou
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China. chuang.wang@mail.hzau.edu.cn.|College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China. chuang.wang@mail.hzau.edu.cn.
authors.forename: Chuang
authors.initials: C
authors.lastname: Wang
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018556:Crops, Agricultural / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism* / Q000494:pharmacology; D010802:Phylogeny; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D010758:Phosphorus; D000135:Acid Phosphatase
keywords: Oryza sativa (rice); purple acid phosphatase (PAP)