title: Characterization of a Crosslinked Elastomeric-Protein Inspired Polypeptide.
abstract: Materials inspired by natural proteins have a great appeal in tissue engineering for their biocompatibility and similarity to extracellular matrix (ECM). Chimeric polypeptides inspired by elastomeric proteins such as silk, elastin, and collagen are of outstanding interest in the field. A recombinant polypeptide constituted of three different blocks, each of them having sequences derived from elastin, resilin, and collagen proteins, was demonstrated to be a good candidate as biomaterial for its self-assembling characteristics and biocompatibility. Herein, taking advantage of the primary amine functionalities present in the linear polypeptide, we crosslinked it with 1,6-hexamethylene-diisocyanate (HMDI). The characterization of the obtained polypeptide was realized by CD spectroscopy, AFM, and SEM microscopies. The obtained results, although not conclusive, demonstrate that the crosslinked polypeptide gave rise to porous networks, thin nanowires, and films not observable for the linear polypeptide. Chirality 28:606-611, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Laboratory of Protein-Inspired Materials, Department of Science, University of Basilicata, Potenza, Italy.
authors.forename: Brigida
authors.initials: B
authors.lastname: Bochicchio
authors.affiliation: Laboratory of Protein-Inspired Materials, Department of Science, University of Basilicata, Potenza, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Bracalello
authors.affiliation: Laboratory of Protein-Inspired Materials, Department of Science, University of Basilicata, Potenza, Italy.
authors.forename: Antonietta
authors.initials: A
authors.lastname: Pepe
mesh_terms: D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004549:Elastin / Q000737:chemistry; D019476:Insect Proteins / Q000737:chemistry; D017953:Isocyanates / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010455:Peptides / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D019476:Insect Proteins; D017953:Isocyanates; D010455:Peptides; D011994:Recombinant Proteins; C015262:1,6-hexamethylene diisocyanate; C030951:resilin; D003094:Collagen; D004549:Elastin
keywords: AFM; SEM; circular dichroism; elastin; resilin