title: Nile Red Loaded PLGA Nanoparticles Surface Modified with Gd-DTPA for Potential Dual-Modal Imaging.
abstract: Here, a novel poly(lactic-co-glycolic acid) (PLGA)-based nanoparticles (NPs) for magnetic resonance (MR) and fluorescence imaging was developed for cell imaging. PLGA NPs loaded with fluorescent dye Nile red (NR) and surface-coated with poly(ethyleneimine) (PEI) were produced in a single step nanoprecipitation process. Diethylenetriamine pentaacetic dianhydride (DTPA) was conjugated to PLGA/NR@PEI NPs through amidation reaction between -COOH of DTPA and -NH2 of PEI, which can chelate gadolinium (Gd3+) as an MR imaging contrast agent. The PLGA/NR@PEI-DTPA-Gd NPs exhibited a uniform particle size of -200 nm and were stable in culture medium. These NPs had a high T relaxivity (R1) of 28.36 mM(-1)S(-1). They did not introduce serious cytotoxicity against A549 lung cancer cells. Furthermore, fluorescence and MR imaging studies on A549 lung cancer cells in vitro revealed that PLGA/NR@PEI-DTPA-Gd NPs can serve as an efficient fluorescence/MR dual-modality imaging nanoprobe.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Li
authors.forename: Chenglin
authors.initials: C
authors.lastname: Li
authors.forename: Weijun
authors.initials: W
authors.lastname: Tong
mesh_terms: D045744:Cell Line, Tumor; D019786:Gadolinium DTPA / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D060328:Magnetic Phenomena; D008279:Magnetic Resonance Imaging / Q000379:methods*; D057054:Molecular Imaging; D064847:Multimodal Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D061848:Optical Imaging / Q000379:methods*; D010078:Oxazines / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013499:Surface Properties
chemical_list: D010078:Oxazines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011094:Polyethyleneimine; D019786:Gadolinium DTPA; C044808:nile red