title: Effectiveness of magnetic fluid hyperthermia against Candida albicans cells.
abstract: Candida albicans is one of the most frequently isolated fungal pathogens causing opportunistic infections in humans. Targeted magnetic fluid hyperthermia (MFH) is a promising method in thermal therapy facilitating selective heating of pathogen cells like C. albicans. In the paper, we used meso-2,3-dimercaptosuccinic acid (DMSA)-coated magnetic nanoparticles (MNPs) and functionalised anti-C. albicans immunomagnetic nanoparticles (IMNPs) to investigate the potential of MFH in combating C. albicans cells in vitro. Using Mössbauer spectroscopy it was found that synthesised MNPs exhibited superparamagnetic phenomena. On the basis of calorimetric experiments, the maximum SAR (specific absorption rate) was found and a proper concentration of MNPs was established to control the temperature. MFH based on both DMSA-coated MNPs and functionalised anti-C. albicans IMNPs was more effective in combating C. albicans cells in vitro than thermostat hyperthermia. Especially promising results were obtained using functionalised IMNPs, which eradicated most of the pathogen colonies at the temperature of 43 °C.
authors.affiliation: a Department of Cell Biology , Maria Curie-Skłodowska University , Lublin , Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Chudzik
authors.affiliation: b Department of Applied Mathematics and Computer Science , University of Life Sciences , Lublin , Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Miaskowski
authors.affiliation: c Faculty of Mathematics, Physics and Computer Science , Maria Curie-Skłodowska University , Lublin , Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Surowiec
authors.affiliation: d Department of Physics , University of Life Sciences in Lublin , Lublin , Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Czernel
authors.affiliation: a Department of Cell Biology , Maria Curie-Skłodowska University , Lublin , Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Duluk
authors.affiliation: e Department of Transporting and Agricultural Machinery , University of Life Sciences , Lublin , Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Marczuk
authors.affiliation: a Department of Cell Biology , Maria Curie-Skłodowska University , Lublin , Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Gagoś
mesh_terms: D002176:Candida albicans / Q000187:drug effects* / Q000276:immunology; D006979:Hyperthermia, Induced; D007074:Immunoglobulin G / Q000008:administration & dosage*; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000648:ultrastructure; D050296:Microbial Viability; D008855:Microscopy, Electron, Scanning; D004113:Succimer / Q000008:administration & dosage*
chemical_list: D007074:Immunoglobulin G; D058185:Magnetite Nanoparticles; D004113:Succimer
keywords: Candida albicans; Mössbauer spectroscopy; electromagnetic field; magnetic fluid hyperthermia; magnetic nanoparticles