title: Clinical Significance of miR-149 in the Survival of Patients with Laryngeal Squamous Cell Carcinoma.
abstract: MicroRNAs (miRNAs) play critical roles in the progression of laryngeal cancer (LC). In this study, we aimed to investigate whether miR-149 is associated with the prognosis of patients with LC. A total of 97 laryngeal squamous cell carcinoma patients who underwent tumor resection were included in our follow-up study. In vitro studies was performed in cancer cell line Hep-2 to explore the antitumor role of miR-149 in LC. We found that the expression of miR-149 was significantly lower in tumor tissues, compared with vocal cord polyp tissues (P < 0.05). Kaplan-Meier analysis revealed that miR-149 expression status is significantly associated with survival duration (log rank test, P < 0.05), and multivariate Cox regression analysis revealed that patients with low miR-149 expression had shorter survival times compared with patients with high miR-149 expression. In vitro studies revealed that the exogenous expression of miRNA-149 inhibits the proliferation of human Hep-2 cells and induces cell apoptosis. Our study suggests that miR-149 expression in laryngeal squamous cell carcinoma tissues is critically associated with the prognosis of patients, and the ectopic expression of miR-149 in Hep-2 cells inhibits proliferation and cell cycle progression.
authors.affiliation: Department of Otorhinolaryngology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Lin
authors.affiliation: Department of Otorhinolaryngology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Otorhinolaryngology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Geng
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Hui-Fang
authors.identifier: 0000-0003-3900-0756
authors.initials: HF
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D014827:Vocal Cords / Q000473:pathology
chemical_list: C551557:MIRN149 microRNA, human; D035683:MicroRNAs