title: β-Adrenergic receptor gene expression in HIV-associated neurocognitive impairment and encephalitis: implications for MOR-1K subcellular localization.
abstract: We previously reported that mRNA expression of the unique alternatively spliced OPRM1 isoform μ-opioid receptor-1K (MOR-1K), which exhibits excitatory cellular signaling, is elevated in HIV-infected individuals with combined neurocognitive impairment (NCI) and HIV encephalitis (HIVE). It has recently been shown that the β2-adrenergic receptor (β2-AR) chaperones MOR-1K, normally localized intracellularly, to the cell surface. Here, we found mRNA expression of the adrenoceptor beta 2 (ADRB2) gene is also elevated in NCI-HIVE individuals, as well as that β2-AR protein expression is elevated in HIV-1-infected primary human astrocytes treated with morphine, and discuss the implications for MOR-1K subcellular localization in this condition.
authors.affiliation: Department of Pharmacology and Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, 23298, USA.|Department of Immunology, Florida International University Herbert Wertheim College of Medicine, 11200 SW 8th Street, Miami, FL, 33199, USA.
authors.forename: Seth M
authors.initials: SM
authors.lastname: Dever
authors.affiliation: Department of Immunology, Florida International University Herbert Wertheim College of Medicine, 11200 SW 8th Street, Miami, FL, 33199, USA.
authors.forename: Myosotys
authors.initials: M
authors.lastname: Rodriguez
authors.affiliation: Department of Pharmacology and Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, 23298, USA. nelhage@fiu.edu.|Department of Immunology, Florida International University Herbert Wertheim College of Medicine, 11200 SW 8th Street, Miami, FL, 33199, USA. nelhage@fiu.edu.
authors.forename: Nazira
authors.initials: N
authors.lastname: El-Hage
mesh_terms: D015526:AIDS Dementia Complex / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018792:Encephalitis, Viral / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005625:Frontal Lobe / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D009020:Morphine / Q000494:pharmacology; D061251:Primary Cell Culture; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics* / Q000378:metabolism; D017450:Receptors, Opioid, mu / Q000235:genetics* / Q000378:metabolism; D066127:White Matter / Q000378:metabolism / Q000473:pathology
chemical_list: C553443:ADRB2 protein, human; C485764:OPRM1 protein, human; D020033:Protein Isoforms; D012333:RNA, Messenger; D018343:Receptors, Adrenergic, beta-2; D017450:Receptors, Opioid, mu; D009020:Morphine
keywords: Central nervous system; HIV encephalitis; HIV-associated neurocognitive disorders; Splice variant; β-Adrenergic receptor; μ-Opioid receptor