title: Direct interaction with ACR11 is necessary for post-transcriptional control of GLU1-encoded ferredoxin-dependent glutamate synthase in leaves.
abstract: Because it plays an essential role in nitrogen (N) assimilation and photorespiration, the glutamine synthetase (GS)/glutamate synthase (GOGAT) system is widely accepted as occupying a central position in leaf N metabolism. However, the regulation of GOGAT at the post-transcriptional level is poorly understood. Here, we show that ACR11, an ACT (acronym for aspartate kinase, chorismate mutase, and TyrA) domain-containing family protein, interacts with Glu1-encoded ferredoxin (Fd)-GOGAT in Arabidopsis chloroplasts. In addition, Arabidopsis acr11 mutants have lost the capability to control Fd-GOGAT levels in response to light/dark diurnal cycles, nitrogen inputs, and changes in photorespiratory activity. Considering that ACR11 has putative glutamine-binding domains, our results indicate that ACR11 is necessary for post-transcriptional control of leaf Glu1-encoded Fd-GOGAT. This regulation takes place through direct interaction of ACR11 and Fd-GOGAT, possibly in an allosteric manner.
authors.affiliation: Institute of Low Temperature Science, Hokkaido University, N19 W8 Kita-Ku, Sapporo 060-0819, Japan.|Japan Core Research for Evolutionary Science and Technology (CREST), JST, N19 W8 Kita-ku, Sapporo 060-0819, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takabayashi
authors.affiliation: Institute of Low Temperature Science, Hokkaido University, N19 W8 Kita-Ku, Sapporo 060-0819, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Niwata
authors.affiliation: Institute of Low Temperature Science, Hokkaido University, N19 W8 Kita-Ku, Sapporo 060-0819, Japan.|Japan Core Research for Evolutionary Science and Technology (CREST), JST, N19 W8 Kita-ku, Sapporo 060-0819, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Tanaka
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism; D005288:Ferredoxins / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism*; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D005288:Ferredoxins; D000594:Amino Acid Oxidoreductases; C042233:glutamate synthase (ferredoxin); C000623406:ACR11 protein, Arabidopsis; D012316:RNA Nucleotidyltransferases; D009584:Nitrogen