title: A comparative study on the in vitro cytotoxic responses of two mammalian cell types to fullerenes, carbon nanotubes and iron oxide nanoparticles.
abstract: The present study was designed to evaluate and compare the time- and dose-dependent cellular response of human periodontal ligament fibroblasts (hPDLFs), and mouse dermal fibroblasts (mDFs) to three different types of nanoparticles (NPs); fullerenes (C60), single walled carbon nanotubes (SWCNTs) and iron (II,III) oxide (Fe3O4) nanoparticles via in vitro toxicity methods, and impedance based biosensor system. NPs were characterized according to their morphology, structure, surface area, particle size distribution and zeta potential by using transmission electron microscopy, X-ray diffraction, Brunauer-Emmett-Teller, dynamic light scattering and zeta sizer analyses. The Mössbauer spectroscopy was used in order to magnetically characterize the Fe3O4NPs. The hPDLFs and mDFs were exposed to different concentrations of the NPs (0.1, 1, 10, 50 and 100 μg/mL) for predetermined time intervals (6, 24 and 48 h) under controlled conditions. Subsequently, NP exposed cells were tested for viability, membrane leakage and generation of intracellular reactive oxygen species. Additional to in vitro cytotoxicity assays, the cellular responses to selected NPs were determined in real time using an impedance based biosensor system. Taken together, information obtained from all experiments suggests that toxicity of the selected NPs is cell type, concentration and time dependent.
authors.affiliation: a Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, Ankara University Stem Cell Institute , Ankara , Turkey and.|b Faculty of Arts and Sciences , Chemistry Department, Hitit University , Çorum , Turkey.
authors.forename: Çiğdem
authors.initials: Ç
authors.lastname: Dönmez Güngüneş
authors.affiliation: a Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, Ankara University Stem Cell Institute , Ankara , Turkey and.
authors.forename: Şükran
authors.initials: Ş
authors.lastname: Şeker
authors.affiliation: a Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, Ankara University Stem Cell Institute , Ankara , Turkey and.
authors.forename: Ayşe Eser
authors.initials: AE
authors.lastname: Elçin
authors.affiliation: a Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, Ankara University Stem Cell Institute , Ankara , Turkey and.
authors.forename: Yaşar Murat
authors.initials: YM
authors.lastname: Elçin
mesh_terms: D000818:Animals; D001681:Biological Assay; D015374:Biosensing Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020405:Dermis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D000067493:Dynamic Light Scattering; D017097:Electric Impedance; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D037741:Fullerenes / Q000633:toxicity*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000633:toxicity*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D037742:Nanotubes, Carbon / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size; D010513:Periodontal Ligament / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D033362:Powder Diffraction; D017382:Reactive Oxygen Species / Q000378:metabolism; D018570:Risk Assessment; D013499:Surface Properties; D013997:Time Factors; D018675:Toxicity Tests / Q000379:methods*; D014961:X-Ray Diffraction
chemical_list: D037741:Fullerenes; D058185:Magnetite Nanoparticles; D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species
keywords: Nanoparticles; carbon nanotube; cytotoxicity; fullerene; impedance based assay; in vitro toxicity; iron (II,III) oxide