title: Monocyte preseeding leads to an increased implant bed vascularization of biphasic calcium phosphate bone substitutes via vessel maturation.
abstract: The present study analyzes the influence of the addition of monocytes to a biphasic bone substitute with two granule sizes (400-700 μm and 500-1000 μm). The majority of the added monocytes was detectable as mononuclear cells, while also low amounts of (chimeric) multinucleated giant cells (MNGCs) were found. No increase in the total number of MNGCs was established, but a significantly increased percent vascularization. Altogether, the results show that the added monocytes become involved in the tissue response to a biomaterial without marked changes in the overall reaction. Monocyte addition enables an increased implant bed vascularization especially via induction of vessel maturation and, thus intervenes positively in the healing reaction to a biomaterial. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2928-2935, 2016.
authors.affiliation: Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany. mike.barbeck@icloud.com.
authors.forename: M
authors.identifier: 0000-0002-3001-1347
authors.initials: M
authors.lastname: Barbeck
authors.affiliation: Institute of Pathology, Repair-Lab, University Medical Center of the Johannes Gutenberg University, Mainz, Germany.
authors.forename: R E
authors.initials: RE
authors.lastname: Unger
authors.affiliation: Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Booms
authors.affiliation: Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Dohle
authors.affiliation: Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany.
authors.forename: R A
authors.initials: RA
authors.lastname: Sader
authors.affiliation: Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany.
authors.forename: C J
authors.initials: CJ
authors.lastname: Kirkpatrick
authors.affiliation: Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Ghanaati
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D015726:Giant Cells / Q000166:cytology; D006801:Humans; D006882:Hydroxyapatites / Q000378:metabolism*; D008422:Materials Testing; D016513:Mice, SCID; D009000:Monocytes / Q000166:cytology*; D018919:Neovascularization, Physiologic; D019736:Prostheses and Implants
chemical_list: D018786:Bone Substitutes; D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate
keywords: addition of monocytes; biphasic bone substitute; multinucleated giant cells; vascularization