title: [Implication of integrin alpha5beta1 signal pathways in proliferation and apoptosis of MCF-7/Dox human breast carcinoma cells].
abstract: In MCF-7/Dox human breast carcinoma cells, down-regulation of integrin alpha5beta1 and inhibition of epidermal growth factor receptor (EGFR) markedly reduced rates of cell proliferation. Mitotic cycle analysis showed that alpha5beta1 down-regulation resulted in cell cycle arrest at the S phase, followed by a significant increase in the population of apoptotic cells (subG1 population). Inhibition of EGFR activity also caused cell cycle arrest at the S-phase but without any increase in the subG1 population. Down-regulation of alpha5beta1 and EGFR inhibition resulted in a significant decrease of cell content of the active (phosphorylated) forms of FAK and Erk protein kinases. The data obtained suggest that alpha5beta1 integrin is implicated in cell growth control via inhibition of apoptotic cell death and through EGFR activation.
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: N I
authors.initials: NI
authors.lastname: Kozlova
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: G E
authors.initials: GE
authors.lastname: Morozevich
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Ushakova
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: N M
authors.initials: NM
authors.lastname: Gevorkian
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: A E
authors.initials: AE
authors.lastname: Berman
mesh_terms: D017209:Apoptosis; D001943:Breast Neoplasms / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism*; D049109:Cell Proliferation; D066246:ErbB Receptors / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D006801:Humans; D039081:Integrin alpha5beta1 / Q000235:genetics / Q000378:metabolism*; D061986:MCF-7 Cells; D016196:S Phase; D015398:Signal Transduction
chemical_list: D039081:Integrin alpha5beta1; D066246:ErbB Receptors; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: cell proliferation; growth factors; integrins; oncogenesis; signaling