title: Construction of a recombinant Lactococcus lactis strain expressing a fusion protein of Omp22 and HpaA from Helicobacter pylori for oral vaccine development.
abstract: OBJECTIVE
  To develop orally administrated anti-Helicobacter pylori vaccination, a Lactococcus lactis strain was genetically constructed for fusion expression of H. pylori protective antigens HpaA and Omp22.
  
  
  RESULTS
  The fusion gene of omp22 and hpaA with an adapter encoding three glycines was cloned from a plasmid pMAL-c2x-omp22-hpaA into Escherichia coli MC1061 and L. lactis NZ3900 successively using a shutter vector pNZ8110. Expression of the fusion gene in L. lactis was induced with nisin resulting in production of proteins with molecular weights of 50 and 28 kDa. Both of them were immunoreactive with mouse anti-H. pylori sera as determined via western blotting. Oral vaccination of BALB/c mice using the L. lactis strain carrying pNZ8110-omp22-hpaA elicited significant systematic humoral immune response (P < 0.05).
  
  
  CONCLUSIONS
  This is the first report showing that a fusion protein of two H. pylori antigens was efficiently expressed in L. lactis with immunogenicity. This is a considerable step towards H. pylori vaccines.
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.|Henan Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang, 453003, China.
authors.forename: Rongguang
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China. gcduan@zzu.edu.cn.|Henan Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang, 453003, China. gcduan@zzu.edu.cn.
authors.forename: Guangcai
authors.initials: G
authors.lastname: Duan
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.
authors.forename: Shuaiyin
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.
authors.forename: Qingtang
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Sun
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Xi
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000276:immunology; D003001:Cloning, Molecular; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009561:Nisin / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D014611:Vaccination
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D008074:Lipoproteins; C089630:Lpp20 protein, Helicobacter pylori; D011993:Recombinant Fusion Proteins; D009561:Nisin; C095977:adhesin A, Helicobacter
keywords: Fusion protein; Helicobactor pylori; Lactococcus lactis; Oral vaccine; hpaA; omp22