title: Modified gene lacZ encoding β-galactosidase as efficient transcriptional reporter for the Yarrowia lipolytica yeast.
abstract: A modified variant of the β-galactosidase gene from Escherichia coli (lacZ) has been obtained. This was designed for building transcriptional reporter constructs, the product of which possesses a higher proteolytic resistance in the Yarrowia lipolytica yeasts. The study of the activity of mitochondrial voltagedependent porin (voltage-dependent anion channel, VDAC) showed a high efficiency of the reporter system based on the designed lacZ reporter compared to the wild-type lacZ gene.
authors.affiliation: Kursk State University, Kursk, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Trubnikova
authors.affiliation: Bach Institute of Biochemistry, Federal Research Center "Fundamentals of Biotechnology,", Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071, Russia. elen_iss@mail.ru.
authors.forename: E P
authors.initials: EP
authors.lastname: Isakova
authors.affiliation: Bach Institute of Biochemistry, Federal Research Center "Fundamentals of Biotechnology,", Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071, Russia.
authors.forename: Yu I
authors.initials: YI
authors.lastname: Deryabina
authors.affiliation: Schmidt Joint Institute of Physics of the Earth, Russian Academy of Sciences, ul. Bol'shaya Gruzinskaya 10, Moscow, 123810, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Nikolaev
mesh_terms: D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D006863:Hydrogen-Ion Concentration; D007763:Lac Operon; D015203:Reproducibility of Results; D050994:Voltage-Dependent Anion Channels / Q000235:genetics / Q000378:metabolism; D025062:Yarrowia / Q000235:genetics* / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D050994:Voltage-Dependent Anion Channels; D001616:beta-Galactosidase