title: A Bioluminescence Assay System for Imaging Metal Cationic Activities in Urban Aerosols.
abstract: A bioluminescence-based assay system was fabricated for an efficient determination of the activities of air pollutants. The following four components were integrated into this assay system: (1) an 8-channel assay platform uniquely designed for simultaneously sensing multiple optical samples, (2) single-chain probes illuminating toxic chemicals or heavy metal cations from air pollutants, (3) a microfluidic system for circulating medium mimicking the human body, and (4) the software manimulating the above system. In the protocol, we briefly introduce how to integrate the components into the system and the application to the illumination of the metal cationic activities in air pollutants.
authors.affiliation: Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), Onogawa 16-1, Tsukuba, Ibaraki, 305-8569, Japan. kimu-sb@aist.go.jp.
authors.forename: Sung-Bae
authors.initials: SB
authors.lastname: Kim
authors.affiliation: Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), Onogawa 16-1, Tsukuba, Ibaraki, 305-8569, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Naganawa
authors.affiliation: Nishihara Electronics co. ltd, 6-8-30, Nishihara, Kashiwa, Chiba, 277-0885, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Murata
authors.affiliation: Nishihara Electronics co. ltd, 6-8-30, Nishihara, Kashiwa, Chiba, 277-0885, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Nakayama
authors.affiliation: Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki, 305-0801, Japan.
authors.forename: Simon
authors.initials: S
authors.lastname: Miller
authors.affiliation: Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki, 305-0801, Japan.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Senda
mesh_terms: D000336:Aerosols / Q000737:chemistry*; D000393:Air Pollutants / Q000032:analysis*; D000818:Animals; D019556:COS Cells; D002412:Cations / Q000032:analysis*; D002522:Chlorocebus aethiops; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008670:Metals / Q000032:analysis*; D052638:Particulate Matter / Q000032:analysis
chemical_list: D000336:Aerosols; D000393:Air Pollutants; D002412:Cations; D008670:Metals; D052638:Particulate Matter
keywords: Air pollutant; Assay system; Bioluminescence; Luciferase; Particulate matter 2.5 (PM2.5); Toxicity