title: Expression of P53 and isoforms in bening and malignant lesions of the head and neck.
abstract: BACKGROUND
  P53, a crucial suppressor of tumor formation, generates multiple isoforms, whose role in disease is still being defined.
  
  
  METHODS
  By immunohistochemistry, we studied the expression of P53 protein and relative isoforms in benign papillomas (PA, n=9), inverted papilloma (IPA, n=10) and squamous cell carcinomas (SCC, n=21).
  
  
  RESULTS
  In all lesions, P53 isoforms were significantly more expressed than P53. Immunoexpression of P53 matched with P53 isoforms in IPA as well as in SCC. Simultaneous immunoexpression of P53 and related isoforms was double in SCC compared to IPA (10% vs 24%), while expression of P53 isoforms was strongly reduced (70% vs 43%). IPA showed the highest percentage of both reactive cases and immunostained cells expressing P53 isoforms.
  
  
  CONCLUSIONS
  We found the higher expression of P53 isoforms in IPA and SCC compared to PA, suggesting their role in local aggressiveness and malignant proliferation in head-neck lesions.
authors.affiliation: Department of Adult and Development Age Human Pathology "Gaetano Barresi", University of Messina, Messina, Italy. mariatrovato@tin.it.|Department of Clinical and Experimental Medicine, Division of Endocrinology, University of Messina, Messina, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Trovato
authors.affiliation: Department of Clinical and Experimental Medicine, Division of Endocrinology, University of Messina, Messina, Italy.
authors.forename: R M
authors.initials: RM
authors.lastname: Ruggeri
authors.affiliation: Department of Adult and Development Age Human Pathology "Gaetano Barresi", Division of Otorhinolaryngology, University of Messina, Messina, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Guzzo
authors.affiliation: Department of Clinical and Experimental Medicine, Division of Endocrinology, University of Messina, Messina, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Certo
authors.affiliation: Department of Economics, University of Messina, Messina, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Alibrandi
authors.affiliation: Department of Adult and Development Age Human Pathology "Gaetano Barresi", Division of Otorhinolaryngology, University of Messina, Messina, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Scifo
authors.affiliation: Department of Adult and Development Age Human Pathology "Gaetano Barresi", University of Messina, Messina, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Scardigno
authors.affiliation: Department of Adult and Development Age Human Pathology "Gaetano Barresi", University of Messina, Messina, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Vitarelli
authors.affiliation: Department of Adult and Development Age Human Pathology "Gaetano Barresi", University of Messina, Messina, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Arena
authors.affiliation: Department of Adult and Development Age Human Pathology "Gaetano Barresi", University of Messina, Messina, Italy.
authors.forename: O
authors.initials: O
authors.lastname: Gambadoro
authors.affiliation: Department of Adult and Development Age Human Pathology "Gaetano Barresi", Division of Otorhinolaryngology, University of Messina, Messina, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Catalano
authors.affiliation: Department of Surgery and Molecular Oncology, University of Dundee, Dundee, UK.
authors.forename: J C
authors.initials: JC
authors.lastname: Bourdon
authors.affiliation: Department of Adult and Development Age Human Pathology "Gaetano Barresi", Division of Otorhinolaryngology, University of Messina, Messina, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Galletti
authors.affiliation: Department of Adult and Development Age Human Pathology "Gaetano Barresi", Division of Otorhinolaryngology, University of Messina, Messina, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Galletti
authors.affiliation: Department of Adult and Development Age Human Pathology "Gaetano Barresi", University of Messina, Messina, Italy.|Interdepartmental Program of Ultrastructural Integrated Diagnostic, AOU "G. Martino", Messina, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Cavallari
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010212:Papilloma / Q000378:metabolism / Q000473:pathology*; D018308:Papilloma, Inverted / Q000378:metabolism / Q000473:pathology*; D020033:Protein Isoforms / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D000077195:Squamous Cell Carcinoma of Head and Neck; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D020033:Protein Isoforms; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53