title: Multiplexed cancer biomarker detection using chip-integrated silicon photonic sensor arrays.
abstract: The analysis of disease-specific biomarker panels holds promise for the early detection of a range of diseases, including cancer. Blood-based biomarkers, in particular, are attractive targets for minimally-invasive disease diagnosis. Specifically, a panel of organ-specific biomarkers could find utility as a general disease surveillance tool enabling earlier detection or prognostic monitoring. Using arrays of chip-integrated silicon photonic sensors, we describe the simultaneous detection of eight cancer biomarkers in serum in a relatively rapid (1 hour) and fully automated antibody-based sandwich assay. Biomarkers were chosen for their applicability to a range of organ-specific cancers, including disease of the pancreas, liver, ovary, breast, lung, colorectum, and prostate. Importantly, we demonstrate that selected patient samples reveal biomarker "fingerprints" that may be useful for a personalized cancer diagnosis. More generally, we show that the silicon photonic technology is capable of measuring multiplexed panels of protein biomarkers that may have broad utility in clinical diagnostics.
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801.
authors.forename: Adam L
authors.initials: AL
authors.lastname: Washburn
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801.
authors.forename: Winnie W
authors.initials: WW
authors.lastname: Shia
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801.
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Lenkeit
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801.
authors.forename: So-Hyun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801.
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Bailey
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D055088:Early Detection of Cancer / Q000379:methods*; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D017785:Photons; D012825:Silicon
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D012825:Silicon