title: Differentiating gastrointestinal stromal tumors from gastric adenocarcinomas and normal mucosae using confocal Raman microspectroscopy.
abstract: Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal neoplasms of the gastrointestinal tract, and gastric adenocarcinomas are a common cancer worldwide. To differentiate GISTs from adenocarcinomas is important because the surgical processes for both are different; the former excises the tumor with negative margins, while the latter requires radical gastrectomy with lymph node dissection. Endoscopy with biopsy is used to distinguish GISTs from adenocarcinomas; however, it may cause tumor bleeding in GISTs. We reported here the confocal Raman microspectroscopy as an effective tool to differentiate GISTs, adenocarcinomas, and normal mucosae. Of 119 patients enrolled in this study, 102 patients underwent gastrectomy (40 GISTs and 62 adenocarcinomas), and 17 patients with benign lesions were obtained as normal mucosae. Raman signals were integrated for 100 s for each spot on the specimen, and 5 to 10 spots, depending on the sample size, were chosen for each specimen. There were significant differences among those tissues as evidenced by different Raman signal responding to phospholipids and protein structures. The spectral data were further processed and analyzed by using principal component analysis. A two-dimensional plot demonstrated that GISTs, adenocarcinomas, and normal gastric mucosae could be effectively differentiated from each other.
authors.affiliation: Tungs' Taichung MetroHarbor Hospital, Division of General Surgery, Department of Surgery, No. 699, Section 8, Taiwan Boulevard, Wuqi, Taichung 43503, TaiwanbNational Chiayi University, Department of Applied Chemistry, No. 300 Syuefu Road, Chiayi City 6000.
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Hsu
authors.affiliation: National Chung Cheng University, Center of Nano Bio-Detection, No. 168, Section 1, University Road, Min-Hsiung Township, Chia-yi County 621, Taiwan.
authors.forename: Chia-Chi
authors.initials: CC
authors.lastname: Huang
authors.affiliation: National Chiayi University, Department of Applied Chemistry, No. 300 Syuefu Road, Chiayi City 60004, Taiwan.
authors.forename: Jeng-Horng
authors.initials: JH
authors.lastname: Sheu
authors.affiliation: HungKuang University, Department of Applied Cosmetology, No. 34, Zhongqi Road, Shalu Township, Taichung County, Taiwan.
authors.forename: Chia-Wen
authors.initials: CW
authors.lastname: Lin
authors.affiliation: Tungs' Taichung MetroHarbor Hospital, Division of Gastroenterology, Department of Internal Medicine, No. 699, Section 8, Taiwan Boulevard, Wuqi, Taichung 43503, Taiwan.
authors.forename: Lien-Fu
authors.initials: LF
authors.lastname: Lin
authors.affiliation: Tungs' Taichung MetroHarbor Hospital, Department of Pathology, No. 699, Section 8, Taiwan Boulevard, Wuqi, Taichung 43503, Taiwan.
authors.forename: Jong-Shiaw
authors.initials: JS
authors.lastname: Jin
authors.affiliation: National Chiayi University, Department of Applied Chemistry, No. 300 Syuefu Road, Chiayi City 60004, Taiwan.
authors.forename: Wenlung
authors.initials: W
authors.lastname: Chen
mesh_terms: D000230:Adenocarcinoma / Q000000981:diagnostic imaging*; D046152:Gastrointestinal Stromal Tumors / Q000000981:diagnostic imaging*; D006801:Humans; D009092:Mucous Membrane / Q000000981:diagnostic imaging*; D015203:Reproducibility of Results; D013059:Spectrum Analysis, Raman; D013274:Stomach Neoplasms / Q000000981:diagnostic imaging*