title: Topical Administration of Acylated Homoserine Lactone Improves Epithelialization of Cutaneous Wounds in Hyperglycaemic Rats.
abstract: Clinicians often experience delayed epithelialization in diabetic patients, for which a high glucose condition is one of the causes. However, the mechanisms underlying delayed wound closure have not been fully elucidated, and effective treatments to enhance epithelialization in patients with hyperglycaemia have not been established. Here we propose a new reagent, acylated homoserine lactone (AHL), to improve the delayed epithelialization due to the disordered formation of a basement membrane of epidermis in hyperglycaemic rats. Acute hyperglycaemia was induced by streptozotocin injection in this experiment. Full thickness wounds were created on the flanks of hyperglycaemic or control rats. Histochemical and immunohistochemical analyses were performed to identify hyperglycaemia-specific abnormalities in epidermal regeneration by comparison between groups. We then examined the effects of AHL on delayed epithelialization in hyperglycaemic rats. Histological analysis showed the significantly shorter epithelializing tissue (P < 0.05), abnormal structure of basement membrane (fragmentation and immaturity), and hypo- and hyperproliferation of basal keratinocytes in hyperglycaemic rats. Treating the wound with AHL resulted in the decreased abnormalities of basement membrane, normal distribution of proliferating epidermal keratinocytes, and significantly promoted epithelialization (P < 0.05) in hyperglycemic rats, suggesting the improving effects of AHL on abnormal epithelialization due to hyperglycemia.
authors.affiliation: Departments of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Huang
authors.affiliation: Departments of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Minematsu
authors.affiliation: Departments of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Kitamura
authors.affiliation: Departments of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: Paes C
authors.initials: PC
authors.lastname: Quinetti
authors.affiliation: Departments of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: Gojiro
authors.initials: G
authors.lastname: Nakagami
authors.affiliation: Departments of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Mugita
authors.affiliation: Department of Advanced Nursing Technology, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Oe
authors.affiliation: Department of Life Support Technology (Molten), Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Noguchi
authors.affiliation: Department of Life Support Technology (Molten), Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Mori
authors.affiliation: Departments of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sanada
mesh_terms: D054742:Acyl-Butyrolactones / Q000008:administration & dosage* / Q000494:pharmacology*; D000287:Administration, Topical; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000473:pathology; D006943:Hyperglycemia / Q000473:pathology* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D062326:Re-Epithelialization / Q000187:drug effects*; D012867:Skin / Q000187:drug effects / Q000293:injuries* / Q000473:pathology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D054742:Acyl-Butyrolactones