title: Expression of HIF-2α and VEGF in Cervical Squamous Cell Carcinoma and Its Clinical Significance.
abstract: CSCC is a systemic disease involving polygenic alteration and multiple steps, and HIF and VEGF are closely associated with tumorigenesis. Specimens surgically resected from 64 cases of CSCC and 22 cases of normal cervical tissue were selected randomly to detect the expression of HIF-2α and VEGF in CSCC for exploring their clinical significance; information regarding the age, lymph node metastasis, and FIGO staging were collected as well; expression of HIF-2α and VEGF was detected by qPCR and immunohistochemistry. We found that the expression of HIF-2α and VEGF mRNA in CSCC was significantly higher than that of normal cervical tissues and showed a positive correlation between them. The positive rates of HIF-2α and VEGF protein expression in CSCC and normal cervical tissues were 93.8% and 18.2%, respectively, with correlation between them. The expression of both HIF-2α and VEGF mRNA did not relate closely to age but the FIGO staging and lymph node metastasis. Compared with the counterpart control group, CSCC tissues with high FIGO staging and lymph node metastasis had a higher level of HIF-2α and VEGF mRNA expression. So, HIF-2α and VEGF were overexpressed in CSCC, which has a great clinical significance for its diagnosis.
authors.affiliation: Jiaxing Maternity and Child Health Care Hospital, Jianxin 314050, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Basic Medical College, Nanchang University, Nanchang 330006, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: The First Clinical Medical College, Nanchang University, Nanchang 330006, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: The Second Clinical Medical College, Nanchang University, Nanchang 330006, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Physiology, Basic Medical College, Nanchang University, Nanchang 330006, China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Physiology, Basic Medical College, Nanchang University, Nanchang 330006, China.
authors.forename: Changshui
authors.initials: C
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D000099162:Endothelial PAS Domain-Containing Protein 1; C467484:VEGFA protein, human