title: Photodynamic inactivation of pathogenic species Pseudomonas aeruginosa and Candida albicans with lutetium (III) acetate phthalocyanines and specific light irradiation.
abstract: Photodynamic inactivation (PDI) is a light-associated therapeutic approach suitable for treatment of local acute infections. The method is based on specific light-activated compound which by specific irradiation and in the presence of molecular oxygen produced molecular singlet oxygen and other reactive oxygen species, all toxic for pathogenic microbial cells. The study presents photodynamic impact of two recently synthesized water-soluble cationic lutetium (III) acetate phthalocyanines (LuPc-5 and LuPc-6) towards two pathogenic strains, namely, the Gram-negative bacterium Pseudomonas aeruginosa and a fungus Candida albicans. The photodynamic effect was evaluated for the cells in suspensions and organized in 48-h developed biofilms. The relatively high levels of uptakes of LuPc-5 and LuPc-6 were determined for fungal cells compared to bacterial cells. The penetration depths and distribution of both LuPcs into microbial biofilms were investigated by means of confocal fluorescence microscopy. The photoinactivation efficiency was studied for a wide concentration range (0.85-30 μM) of LuPc-5 and LuPc-6 at a light dose of 50 J cm-2from red light-emitting diode (LED; 665 nm). The PDI study on microbial biofilms showed incomplete photoinactivation (<3 logs) for the used gentle drug-light protocol.
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev str., Bl. 9, 1113, Sofia, Bulgaria. mantareva@yahoo.com.
authors.forename: Vanya
authors.initials: V
authors.lastname: Mantareva
authors.affiliation: The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev str., Bl. 26, 1113, Sofia, Bulgaria.
authors.forename: Vesselin
authors.initials: V
authors.lastname: Kussovski
authors.affiliation: Department of Chemistry, Gebze Technical University, P. O. Box 141, Gebze, Kocaeli, 41400, Turkey.
authors.forename: Mahmut
authors.initials: M
authors.lastname: Durmuş
authors.affiliation: Institute of Electronics, Bulgarian Academy of Sciences, Tzarigradsko chaussee blvd, 1784, Sofia, Bulgaria.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Borisova
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev str., Bl. 9, 1113, Sofia, Bulgaria.
authors.forename: Ivan
authors.initials: I
authors.lastname: Angelov
mesh_terms: D000085:Acetates / Q000737:chemistry / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000378:metabolism / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D007211:Indoles / Q000378:metabolism / Q000494:pharmacology*; D054833:Isoindoles; D054023:Lasers, Semiconductor; D008187:Lutetium / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D017319:Photosensitizing Agents / Q000378:metabolism / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*
chemical_list: D000085:Acetates; D000890:Anti-Infective Agents; D007211:Indoles; D054833:Isoindoles; D017319:Photosensitizing Agents; D008187:Lutetium; C013647:phthalocyanine
keywords: Blue diode laser; Candida albicans; Lutetium (III) phthalocyanines; Photodynamic inactivation; Pseudomonas aeruginosa; Red light-emitting diode