title: Use of Cupriavidus basilensis-aided bioabatement to enhance fermentation of acid-pretreated biomass hydrolysates by Clostridium beijerinckii.
abstract: Lignocellulose-derived microbial inhibitors (LDMICs) prevent efficient fermentation of Miscanthus giganteus (MG) hydrolysates to fuels and chemicals. To address this problem, we explored detoxification of pretreated MG biomass by Cupriavidus basilensis ATCC(®)BAA-699 prior to enzymatic saccharification. We document three key findings from our test of this strategy to alleviate LDMIC-mediated toxicity on Clostridium beijerinckii NCIMB 8052 during fermentation of MG hydrolysates. First, we demonstrate that growth of C. basilensis is possible on furfural, 5-hydroxymethyfurfural, cinnamaldehyde, 4-hydroxybenzaldehyde, syringaldehyde, vanillin, and ferulic, p-coumaric, syringic and vanillic acid, as sole carbon sources. Second, we report that C. basilensis detoxified and metabolized ~98 % LDMICs present in dilute acid-pretreated MG hydrolysates. Last, this bioabatement resulted in significant payoffs during acetone-butanol-ethanol (ABE) fermentation by C. beijerinckii: 70, 50 and 73 % improvement in ABE concentration, yield and productivity, respectively. Together, our results show that biological detoxification of acid-pretreated MG hydrolysates prior to fermentation is feasible and beneficial.
authors.affiliation: Department of Animal Sciences, Ohio State Agricultural Research and Development Center (OARDC), The Ohio State University, 305 Gerlaugh Hall, 1680 Madison Avenue, Wooster, OH, 44691, USA.
authors.forename: Chidozie Victor
authors.initials: CV
authors.lastname: Agu
authors.affiliation: Renewable Energy Program, Agricultural Technical Institute, The Ohio State University, 1328 Dover Road, Wooster, OH, 44691, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Ujor
authors.affiliation: Department of Chemistry and Biochemistry, Center for RNA Biology, The Ohio State University, 484 West 12th Avenue, Columbus, OH, 43210, USA.
authors.forename: Venkat
authors.initials: V
authors.lastname: Gopalan
authors.affiliation: Department of Animal Sciences, Ohio State Agricultural Research and Development Center (OARDC), The Ohio State University, 305 Gerlaugh Hall, 1680 Madison Avenue, Wooster, OH, 44691, USA. ezeji.1@osu.edu.
authors.forename: Thaddeus Chukwuemeka
authors.identifier: 0000-0002-8384-895X
authors.initials: TC
authors.lastname: Ezeji
mesh_terms: D000096:Acetone / Q000378:metabolism; D018533:Biomass; D000440:Butanols / Q000378:metabolism; D046970:Clostridium beijerinckii / Q000378:metabolism*; D049921:Cupriavidus / Q000254:growth & development / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D008031:Lignin / Q000378:metabolism; D006109:Poaceae / Q000378:metabolism
chemical_list: D000440:Butanols; C036909:lignocellulose; D000096:Acetone; D000431:Ethanol; D008031:Lignin
keywords: Bioabatement; Butanol; Clostridium beijerinckii; Cupriavidus basilensis; Lignocellulose