title: Getting the whole picture: High content screening using three-dimensional cellular model systems and whole animal assays.
abstract: Phenotypic or High Content Screening (HCS) is becoming more widely used for primary screening campaigns in drug discovery. Currently the vast majority of HCS campaigns are using cell lines grown in well-established monolayer cultures (2D tissue culture). There is widespread recognition that the more biologically relevant 3D tissue culture technologies such as spheroids and organoids and even whole animal assays will eventually be run as primary HCS. Upgrading the IT infrastructure to cope with the increase in data volumes requires investments in hardware (and software) and this will be manageable. However, the main bottleneck for the effective adoption and use of 3D tissue culture and whole animal assays in HCS is anticipated to be the development of software for the analysis of 3D images. In this review we summarize the current state of the available software and how they may be applied to analyzing 3D images obtained from a HCS campaign. © 2016 International Society for Advancement of Cytometry.
authors.affiliation: Bioinformatics Image Core, MRC Laboratory for Molecular Cell Biology, University College London, London, United Kingdom.
authors.forename: Janos
authors.initials: J
authors.lastname: Kriston-Vizi
authors.affiliation: HDC GmbH, Byk Gulden Strasse 2, Konstanz, Germany.
authors.forename: Horst
authors.initials: H
authors.lastname: Flotow
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D055808:Drug Discovery; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional; D012984:Software
keywords: 3D tissue culture; automated image analysis; high content screening; phenotypic assays