title: Transforming growth factor-β activated long non-coding RNA ATB plays an important role in acute rejection of renal allografts and may impacts the postoperative pharmaceutical immunosuppression therapy.
abstract: OBJECTIVE
  Long noncoding RNAs (lncRNAs) are novel intracellular noncoding ribonucleotides regulating the genome and proteome. The lncRNA activated by transforming growth factor β (TGF-β) (lncRNA-ATB) was discovered as a prognostic factor in hepatocellular carcinoma, gastric cancer, and colorectal cancer. However, little is known about the role of lncRNA-ATB in renal transplantation. This study aimed to assess lncRNA-ATB expression in renal transplant patients with acute kidney injury and explore its role in postoperative pharmaceutical immunosuppression therapy.
  
  
  METHODS
  We detected lncRNA-ATB expression in the kidney biopsies of a cohort of 72 patients with renal allograft rejection and 36 control transplant patients. lncRNA-ATB were overexpressed from lentiviral vectors in renal cells.
  
  
  RESULTS
  We found that lncRNA-ATB was remarkably upregulated in patients with acute rejection compared with controls. Meanwhile, lncRNA-ATB could influence the kidney cell phenotypes and impact the nephrotoxicity of immunosuppressive drug.
  
  
  CONCLUSIONS
  In conclusion, lncRNA-ATB are strongly altered in patients with acute rejection and may serve as a novel biomarker of acute kidney rejection, identifying patients with acute rejection and predicting loss of kidney function.
authors.affiliation: Division of Organ Transplantation, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Division of Urology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Yehui
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Division of Organ Transplantation, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Huang
authors.affiliation: Division of Urology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Division of Organ Transplantation, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Lizhong
authors.initials: L
authors.lastname: Chen
authors.affiliation: Division of Organ Transplantation, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Na
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D064591:Allografts; D017209:Apoptosis / Q000187:drug effects; D065095:Calcineurin Inhibitors / Q000009:adverse effects*; D016022:Case-Control Studies; D049109:Cell Proliferation / Q000187:drug effects; D016572:Cyclosporine / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006084:Graft Rejection / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D057809:HEK293 Cells; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D020128:Inhibitory Concentration 50; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D016896:Treatment Outcome; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D065095:Calcineurin Inhibitors; D007166:Immunosuppressive Agents; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; C000619854:long non-coding RNA ATB, human; D016572:Cyclosporine
keywords: acute rejection; apoptosis; calcineurin inhibitors; cell proliferation; lncRNA