title: Fluorescence-Activated Cell Sorting (FACS) Protocol for Podocyte Isolation in Adult Zebrafish.
abstract: Zebrafish is becoming a very important model for studying human diseases. The conserved structure of the nephrons in the kidney allows the user to answer questions relating to study human kidney disorders. Wt1a-expressing podocytes are the most important cells within the glomeruli of adult zebrafish. In order to understand the molecular characteristics of these cells, within damage models, we have established a method for isolating them.
authors.affiliation: Leibniz Institute for Age -Fritz Lipmann Institute, Beutenbergstrasse 11, 07745, Jena, Germany.
authors.forename: Thomas J D
authors.initials: TJ
authors.lastname: Bates
authors.affiliation: Leibniz Institute for Age -Fritz Lipmann Institute, Beutenbergstrasse 11, 07745, Jena, Germany.
authors.forename: Uta
authors.initials: U
authors.lastname: Naumann
authors.affiliation: Leibniz Institute for Age -Fritz Lipmann Institute, Beutenbergstrasse 11, 07745, Jena, Germany. cenglert@fli-leibniz.de.|Friedrich Schiller University, Fürstengraben 1, 07743, Jena, Germany. cenglert@fli-leibniz.de.
authors.forename: Christoph
authors.initials: C
authors.lastname: Englert
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D050199:Podocytes / Q000166:cytology* / Q000378:metabolism; D025721:WT1 Proteins / Q000378:metabolism; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000378:metabolism
chemical_list: D025721:WT1 Proteins; C000594123:Wt1a protein, zebrafish; D029961:Zebrafish Proteins
keywords: FACS; Podocytes; RNA isolation; Wt1a; qPCR