title: Immune response of the Antarctic teleost Trematomus bernacchii to immunization with Psychrobacter sp. (TAD1).
abstract: Adult Trematomus bernacchii have been immunized intraperitoneally with heat-killed cells of the Antarctic marine bacterium Psychrobacter sp. (TAD1) up to 60 days. After immunizations and sampling at various times, fish sera were tested for specific IgM by ELISA, and different tissues (head kidney and spleen) were investigated for transcription of master genes of the acquired immune response (IgM, IgT, TRβ, TRγ). Results from ELISA assays showed a time-dependent induction of specific serum anti-TAD1 IgM, and western blot analysis of TAD1 lysates probed with fish sera revealed enhanced immunoreactivity in immunized animals compared to controls. Quantitative PCR analysis of transcripts coding for IgM, IgT, TRβ, TRγ was performed in T. bernacchii tissues to assess basal expression, and then on cDNAs of cells from head kidney and spleen of fish injected for 8, 24, and 72 h with inactivated TAD1. The results showed a differential basal expression of transcripts in the examined tissues, and a time-dependent strong up-regulation of IgT, TRβ, TRγ genes upon in vivo stimulation with TAD1. These results represent a first in vivo study on the mounting of a specific immune response in an Antarctic teleost species.
authors.affiliation: Università della Tuscia, Dipartimento per l'Innovazione Biologica, Agroalimentare e Forestale, Viterbo, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Buonocore
authors.affiliation: Università della Tuscia, Dipartimento per l'Innovazione Biologica, Agroalimentare e Forestale, Viterbo, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Bernini
authors.affiliation: Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Napoli, Italy.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Coscia
authors.affiliation: Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Napoli, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Giacomelli
authors.affiliation: Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Napoli, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: de Pascale
authors.affiliation: Università della Tuscia, Dipartimento per l'Innovazione Biologica, Agroalimentare e Forestale, Viterbo, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Randelli
authors.affiliation: Università della Tuscia, Dipartimento per l'Innovazione Biologica, Agroalimentare e Forestale, Viterbo, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Stocchi
authors.affiliation: Università della Tuscia, Dipartimento per l'Innovazione Biologica, Agroalimentare e Forestale, Viterbo, Italy. Electronic address: scapigg@unitus.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Scapigliati
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D060226:Head Kidney / Q000276:immunology; D056724:Immunity, Humoral; D007114:Immunization / Q000662:veterinary*; D007075:Immunoglobulin M / Q000097:blood; D007274:Injections, Intraperitoneal / Q000662:veterinary; D045828:Moraxellaceae Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D010473:Perciformes; D044096:Psychrobacter / Q000276:immunology*; D013154:Spleen / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D029941:Fish Proteins; D007075:Immunoglobulin M
keywords: IgM; IgT; Immunization; Lymphocytes; Psychrobacter TAD1; Trematomus bernacchii