title: Styrene production from a biomass-derived carbon source using a coculture system of phenylalanine ammonia lyase and phenylacrylic acid decarboxylase-expressing Streptomyces lividans transformants.
abstract: To produce styrene from a biomass-derived carbon source, Streptomyces lividans was adopted as a host strain. The gene encoding ferulic acid decarboxylase from Saccharomyces cerevisiae (FDC1) was introduced into S. lividans, and the resulting S. lividans transformant successfully expressed FDC1 and converted trans-cinnamic acid (CA) to styrene. A key factor in styrene production using microbes is the recovery of volatile styrene. In the present study, we selected polystyrene resin beads XRD-4 as the absorbent agent to recover styrene produced using S. lividans transformants, which enabled recovery of styrene from the culture broth. For styrene production from biomass-derived carbon sources, S. lividans/FDC1 was cultured together with S. lividans/p-encP, which we previously reported as a CA-producing S. lividans strain. This coculture system combined with the recovery of styrene using XAD-4 allowed the production of styrene from glucose, cellobiose, or xylo-oligosaccharide, respectively.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Fujiwara
authors.affiliation: Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Noda
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan; Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan. Electronic address: akondo@kobe-u.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism; D002262:Carboxy-Lyases / Q000235:genetics* / Q000378:metabolism*; D002475:Cellobiose / Q000378:metabolism; D002934:Cinnamates / Q000378:metabolism; D018920:Coculture Techniques; D005947:Glucose / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods; D009844:Oligosaccharides / Q000378:metabolism; D030781:Organisms, Genetically Modified; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013302:Streptomyces / Q000235:genetics; D048170:Streptomyces lividans / Q000235:genetics / Q000378:metabolism; D020058:Styrene / Q000378:metabolism*
chemical_list: D002934:Cinnamates; D005965:Glucuronates; D009844:Oligosaccharides; C570991:xylooligosaccharide; C029010:cinnamic acid; D002475:Cellobiose; D020058:Styrene; D002244:Carbon; D002262:Carboxy-Lyases; C087194:phenylacrylic acid decarboxylase; D010650:Phenylalanine Ammonia-Lyase; D005947:Glucose
keywords: Cellobiose; Coculture; Streptomyces lividans; Styrene; Xylo-oligosaccharide