title: The effect of histidine modification on copper-dependent lipid peroxidation in human low-density lipoprotein.
abstract: Lipid peroxidation and subsequent oxidative modification of low-density lipoprotein (LDL) have been implicated as causal events in atherosclerosis. Cu(2+) may play an important role in LDL oxidation by binding to histidine residues of apolipoprotein B-100 (apo B) and initiating and propagating lipid peroxidation. To investigate the role of histidine residues, we used diethylpyrocarbonate (DEPC), a lipid-soluble histidine-specific modifying reagent. When LDL (0.1 mg protein/ml, or 0.2 µM) was incubated with DEPC (1 mM), at least 76 ± 7% of the histidine residues in apo B were modified. Treatment of LDL with DEPC led to an increase in the rate of Cu(2+)-induced initiation of lipid peroxidation (Ri), but a significant decrease in the rate of propagation. These changes resulted in an overall increased resistance of LDL to oxidation, with a significantly increased lag phase preceding the propagation phase of lipid peroxidation. In contrast to DEPC, ascorbate completely prevented the initiation of LDL oxidation (Ri = 0). Our data indicate that there are two types of copper/histidine binding sites on apo B: those facing the lipid core of the LDL particle, which mediate the propagation of lipid peroxidation and are modified by DEPC; and those found on the surface of the LDL particle exposed to the aqueous environment, which are responsible for mediating the initiation of lipid peroxidation and are modifiable by ascorbate in the presence of Cu(2+).
authors.affiliation: a Evans Memorial Department of Medicine , Whitaker Cardiovascular Institute, Boston University School of Medicine , Boston , MA , USA .
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.affiliation: a Evans Memorial Department of Medicine , Whitaker Cardiovascular Institute, Boston University School of Medicine , Boston , MA , USA .
authors.forename: B
authors.initials: B
authors.lastname: Frei