title: Characterization and antioxidant activities of polysaccharides from the leaves of Lilium lancifolium Thunb.
abstract: In this study, LLP-1, LLP-2, and LLP-3 three novel polysaccharide fractions were purified from the leaves of Lilium lancifolium, and their physicochemical characterizations and antioxidant properties were investigated by chemical methods, high performance gel permeation chromatography (HPGPC), high performance liquid chromatography (HPLC), fourier transform infrared spectrometry (FT-IR), scanning electron microscopy (SEM), and DPPH radical assay, hydroxyl radical assay, superoxide radical assay and ferrous ion chelating assay, respectively. Results showed that LLP-1, LLP-2, and LLP-3 had low protein and uronic acid contents, meanwhile, their weight-average molecular weight were estimated to be 2.25×106, 2.02×106, and 2.08×106Da, respectively. Mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose glucose and galactose were the major monosaccaherides components presenting in the polysaccharide fractions. Three polysaccharide fractions were not observed triple-helical conformation, while possessed variant surface structure. In addition, three polysaccharide fractions all exhibited significantly scavenging activities against free radical and chelate Fe2+in vitro.
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xu
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Handong
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Bulei
authors.initials: B
authors.lastname: Wang
authors.affiliation: Dazhou Institute of Agricultural Sciences, Da'zhou 635000, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Fu
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhou
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China. Electronic address: dcb@sichuan.edu.cn.
authors.forename: Chunbang
authors.initials: C
authors.lastname: Ding
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification*; D027762:Lilium / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides
keywords: Antioxidant activities; Characterization; Lilium lancifolium Thunb.; Polysaccharides