title: Exploiting Chromatin Biology to Understand Immunology.
abstract: The activation of immune responses relies on variations of a common rule where immune cells that are able to sense infections produce one set of cytokines to induce lymphocytes to produce another set of cytokines, which in turn activate the appropriate effector responses. This multitiered immune response is in fact a remarkable showcase of different ways the same genome can be used to facilitate cellular communications. Here, we review next-generation sequencing methods enabling us to map the differential usage of our genome in primary immune cells.
authors.affiliation: Institute for Immunology, Pereleman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.
authors.forename: J L
authors.initials: JL
authors.lastname: Johnson
authors.affiliation: Institute for Immunology, Pereleman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States. Electronic address: vahedi@mail.med.upenn.edu.
authors.forename: G
authors.initials: G
authors.lastname: Vahedi
mesh_terms: D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D019175:DNA Methylation; D057890:Epigenomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D042421:Histone Code; D006801:Humans; D007111:Immunity, Cellular; D012984:Software
chemical_list: D002843:Chromatin
keywords: ATAC-seq; ChIP-seq; Cytokines; DNase-seq; Enhancers; Epigenome; Immunology; Next-generation sequencing; T cells