title: Molecular cloning and expression vector construction of bovine TRIM28.
abstract: The bovine TRIM28 gene was amplified from ovary tissue by using RT-PCR. The TRIM28 gene was inserted into the eukaryotic expression vector pIRES2-EGFP and transfected into bovine fetal fibroblasts by using Lipofectamine 3000. TRIM28 mRNA and protein were detected by fluorescence microscope and western blotting. The results showed that the full length of TRIM28 was cloned and pIRES2-EGFP-TRIM28 was constructed successfully. EGFP expression was observed, and the pIRES2-EGFP-TRIM28 transfected group expressed more TRIM28 protein than that by the pIRES2-EGFP group. The TIMR28 gene has been successfully transferred into bovine fetal fibroblasts.
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
authors.forename: X
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Zhai
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
authors.forename: M L
authors.initials: ML
authors.lastname: Zhang
authors.affiliation: Chang Chun Medical College, Changchun, Jilin, China.
authors.forename: B H
authors.initials: BH
authors.lastname: Song
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
authors.forename: Y R
authors.initials: YR
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
authors.forename: S B
authors.initials: SB
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Dong
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Su
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
authors.forename: C F
authors.initials: CF
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
authors.forename: H X
authors.initials: HX
authors.lastname: Ma
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
authors.forename: W M
authors.initials: WM
authors.lastname: Luan
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D010053:Ovary / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D012097:Repressor Proteins