title: Inhibition of Fibrillar Assemblies of l-Phenylalanine by Crown Ethers: A Potential Approach toward Phenylketonuria.
abstract: In this article, our aim is to investigate the interaction of l-phenylalanine (l-Phe) fibrils with crown ethers (CEs). For this purpose, two different CEs (15-Crown-5 (15C5) and 18-Crown-6 (18C6)) were used. Interestingly, we have observed that both CEs have the ability to arrest fibril formation. However, 18C6 was found to be a better candidate compared to 15C5. Field emission scanning electron microscopy and fluorescence lifetime imaging microscopy were used to monitor the fibril-arresting kinetics of CEs. The arresting process was further confirmed by fluorescence correlation spectroscopy and nuclear magnetic resonance studies.
authors.affiliation: Department of Chemistry, Indian Institute of Technology , Kharagpur 721302, WB, India.
authors.forename: Debasis
authors.initials: D
authors.lastname: Banik
authors.affiliation: Department of Chemistry, Indian Institute of Technology , Kharagpur 721302, WB, India.
authors.forename: Rupam
authors.initials: R
authors.lastname: Dutta
authors.affiliation: Department of Chemistry, Indian Institute of Technology , Kharagpur 721302, WB, India.
authors.forename: Pavel
authors.initials: P
authors.lastname: Banerjee
authors.affiliation: Department of Chemistry, Indian Institute of Technology , Kharagpur 721302, WB, India.
authors.forename: Sangita
authors.initials: S
authors.lastname: Kundu
authors.affiliation: Department of Chemistry, Indian Institute of Technology , Kharagpur 721302, WB, India.
authors.forename: Nilmoni
authors.initials: N
authors.lastname: Sarkar
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002942:Circular Dichroism; D043844:Crown Ethers / Q000737:chemistry* / Q000494:pharmacology; D007700:Kinetics; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010649:Phenylalanine / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism; D010661:Phenylketonurias / Q000188:drug therapy / Q000378:metabolism; D066244:Proton Magnetic Resonance Spectroscopy; D011714:Pyrans / Q000737:chemistry; D013343:Styrenes / Q000737:chemistry
chemical_list: D000682:Amyloid; D043844:Crown Ethers; D011714:Pyrans; D013343:Styrenes; C042148:15-crown-5; D010649:Phenylalanine; C015762:18-crown-6; C082825:4-(dicyanomethylene)-2-methyl-6-(4-(dimethylamino)styryl)-4H-pyran