title: Prevalence of Enteric Protozoan Oocysts with Special Reference to Sarcocystis cruzi among Fecal Samples of Diarrheic Immunodeficient Patients in Iran.
abstract: The genus Sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. It might be expected that species for which humans are the final host (Sarcocystis hominis and Sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. This study aimed to address how to detect and differentiate Sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patients in Shiraz, Iran. Diarrheal samples of 741 immunodeficient patients with recurrent persistent or chronic diarrhea were examined by microscopy and molecular biological analysis. Oocysts-positive samples were 68 Cryptosporidium spp., 9 Cystoisospora belli (syn. Isospora belli), 2 Cyclospora cayetanensis, and 15 microsporidia (Enterocytozoon bieneusi). Sarcocystis-like sporocysts found from a woman were identified as Sarcocystis cruzi through 18S rDNA amplification and phylogenetic analysis. To the best of our knowledge, this is the first report of S. cruzi from a human.
authors.affiliation: HIV/AIDS Research Center, Fasa University of Medical Sciences, Fasa, Iran.|Department of Microbiology, Fasa University of Medical Sciences, Fasa, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Agholi
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Shahrbanou Naderi
authors.initials: SN
authors.lastname: Shahabadi
authors.affiliation: Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Motazedian
authors.affiliation: Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Gholam Reza
authors.initials: GR
authors.lastname: Hatam
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology* / Q000469:parasitology; D005243:Feces / Q000469:parasitology*; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D007492:Iran / Q000453:epidemiology; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D033661:Oocysts; D010271:Parasites / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D015995:Prevalence; D011528:Protozoan Infections / Q000453:epidemiology* / Q000469:parasitology; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S
keywords: AIDS; PCR; Sarcocystis cruzi; diarrhea; human; sporocyst