title: Identification of single nucleotide polymorphism markers associated with resistance to bruchids (Callosobruchus spp.) in wild mungbean (Vigna radiata var. sublobata) and cultivated V. radiata through genotyping by sequencing and quantitative trait locus analysis.
abstract: Bruchid beetles are an important storage pest of grain legumes. Callosobruchus sp. infect mungbean (Vigna radiata) at low levels in the field, multiply during grain storage and can destroy seed stocks in a few months. Resistance against bruchid beetles has been found in wild mungbean V. radiata var. sublobata TC1966 and in cultivated mungbean line V2802.
  
  
  
  Bruchid resistance data were obtained from recombinant inbred line populations TC1966 (V. radiata var. sublobata) × NM92 (F12) and V2802 (V. radiata) × NM94 (F7). More than 6,000 single nucleotide polymorphic markers were generated through genotyping by sequencing (GBS) for each of these populations and were used to map bruchid resistance genes. One highly significant quantitative trait locus (QTL) associated with bruchid resistance was mapped to chromosome 5 on genetic maps of both populations, suggesting that TC1966 and V2802 contain the same resistance locus. Co-segregation of all markers associated with resistance indicated the presence of only one major resistance QTL on chromosome 5, while QTL analysis based on physical map positions of the markers suggested the presence of multiple QTLs on different chromosomes. The diagnostic capacity of the identified molecular markers located in the QTL to correctly predict resistance was up to 100 %.
  
  
  
  Molecular markers tightly linked to bruchid resistance loci of two different mungbean resistance sources were developed and validated. These markers are highly useful for developing resistant lines.
authors.affiliation: Biotechnology/Molecular Breeding, World Vegetable Center, 60 Yi Min Liao, Shanhua, Tainan, 74151, Taiwan. roland.schafleitner@worldveg.org.
authors.forename: Roland
authors.initials: R
authors.lastname: Schafleitner
authors.affiliation: Biotechnology/Molecular Breeding, World Vegetable Center, 60 Yi Min Liao, Shanhua, Tainan, 74151, Taiwan.
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: Huang
authors.affiliation: Biotechnology/Molecular Breeding, World Vegetable Center, 60 Yi Min Liao, Shanhua, Tainan, 74151, Taiwan.
authors.forename: Shui-Hui
authors.initials: SH
authors.lastname: Chu
authors.affiliation: Legume Breeding, World Vegetable Center, 60 Yi Min Liao, Shanhua, Tainan, 74151, Taiwan.
authors.forename: Jo-Yi
authors.initials: JY
authors.lastname: Yen
authors.affiliation: Biotechnology/Molecular Breeding, World Vegetable Center, 60 Yi Min Liao, Shanhua, Tainan, 74151, Taiwan.
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Lin
authors.affiliation: Legume Breeding, World Vegetable Center, 60 Yi Min Liao, Shanhua, Tainan, 74151, Taiwan.
authors.forename: Miao-Rong
authors.initials: MR
authors.lastname: Yan
authors.affiliation: Information Technology, World Vegetable Center, 60 Yi Min Liao, Shanhua, Tainan, 74151, Taiwan.
authors.forename: Bharath
authors.initials: B
authors.lastname: Krishnan
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, 115, Taiwan.
authors.forename: Mao-Sen
authors.initials: MS
authors.lastname: Liu
authors.affiliation: Horticulture and Landscape Architecture / Horticulture Section, Experimental Farm, College of Bio-Resources and Agriculture National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan.
authors.forename: Hsiao-Feng
authors.initials: HF
authors.lastname: Lo
authors.affiliation: Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan.
authors.forename: Chien-Yu
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, 115, Taiwan.
authors.forename: Long-Fang O
authors.initials: LF
authors.lastname: Chen
authors.affiliation: Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan.
authors.forename: Dung-Chi
authors.initials: DC
authors.lastname: Wu
authors.affiliation: Division of Agricultural Biodiversity, Plant Resources Center, Plant Resources Center, Vietnam Academy of Agriculture Sciences, An Khanh, Hoai Duc, Ha Noi, Vietnam.
authors.forename: Thu-Giang Thi
authors.initials: TG
authors.lastname: Bui
authors.affiliation: Entomology, World Vegetable Center, 60 Yi Min Liao, Shanhua, Tainan, 74151, Taiwan.
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Ramasamy
authors.affiliation: Depertment of Agronomy, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan.
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Tung
authors.affiliation: Legume Breeding, World Vegetable Center South Asia, ICRISAT Campus, Patancheru, 502 324, Hyderabad, Telangana, India.
authors.forename: Ramakrishnan
authors.initials: R
authors.lastname: Nair
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000502:physiology*; D005838:Genotype; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA; D000070658:Vigna / Q000235:genetics* / Q000276:immunology / Q000469:parasitology