title: Expression of recombinant human anti-TNF-α scFv-Fc in Arabidopsis thaliana seeds.
abstract: Recombinant human anti-tumor necrosis factor (TNF)-α scFv-Fc was expressed in TKO mutant Arabidopsis thaliana seeds using plant-specific codons. Immunoblotting using a human IgG1 antibody detected the expression of anti-TNF-α proteins in plants. Results from qRT-PCR analysis demonstrated that the time of harvest significantly affected the protein yield and quality. Our results indicate that the Phaseolus vulgaris β-phaseolin promoter directed anti-TNF-α scFv-Fc expression in A. thaliana seeds, with a maximum yield obtained at 20-days of development. Although the yield of anti-TNF-α scFv-Fc protein was not very high, accumulation of recombinant proteins in seeds is an attractive and simple method that can be used to purify biologically active anti-TNF-α scFv-Fc.
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Ji Lin, China.
authors.forename: N
authors.initials: N
authors.lastname: Yao
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Ji Lin, China.
authors.forename: L
authors.initials: L
authors.lastname: Ai
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Ji Lin, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Dong
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Ji Lin, China.
authors.forename: X M
authors.initials: XM
authors.lastname: Liu
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Ji Lin, China.
authors.forename: D Z
authors.initials: DZ
authors.lastname: Wang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Ji Lin, China.
authors.forename: N
authors.initials: N
authors.lastname: Wang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Ji Lin, China.
authors.forename: X W
authors.initials: XW
authors.lastname: Li
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Ji Lin, China.
authors.forename: F W
authors.initials: FW
authors.lastname: Wang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Ji Lin, China.
authors.forename: Xk
authors.initials: X
authors.lastname: Li
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Ji Lin, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Li
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Ji Lin, China.
authors.forename: C
authors.initials: C
authors.lastname: Jiang
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D007141:Immunoglobulin Fc Fragments / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D057127:Single-Chain Antibodies / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D019076:Transgenes; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
chemical_list: D007141:Immunoglobulin Fc Fragments; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; D014409:Tumor Necrosis Factor-alpha