title: WT1-Associated Protein-Protein Interaction Networks.
abstract: Tumor-suppressor protein Wt1 has been shown to interact with specific proteins that influence its function. These protein interactions have been identified as direct individual interactions but with the potential to exist as a part of a multiprotein complex. In order to obtain the global proteome interaction map of Wt1, an unbiased label-free endogenous immunoprecipitation was performed followed by mass spectrometry to identify protein interactions that are Wt1 centric. This chapter details the different techniques that have been used to identify and characterize Wt1-interacting proteins.
authors.affiliation: Medical Research Council-Human Genetics Unit, Institute of Genetics and Molecular Medicine, Western General Hospital, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, Scotland, UK. ruthrothaselvi.bharathavikru@igmm.ed.ac.uk.
authors.forename: Ruthrothaselvi
authors.initials: R
authors.lastname: Bharathavikru
authors.affiliation: Systems Biology Ireland, Conway Institute, Belfield, Dublin 4, Ireland.
authors.forename: Alex
authors.initials: A
authors.lastname: von Kriegsheim
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D040901:Proteomics; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D025721:WT1 Proteins / Q000378:metabolism*
chemical_list: D025721:WT1 Proteins; C583751:WT1 protein, human
keywords: Immunoprecipitation; Mass spectrometry; Protein interaction; Proteome