title: Effects of flooding stress in 'Micro-Tom' tomato plants transformed with different levels of mitochondrial sHSP23.6.
abstract: Soil flooding is an environmental stressor for crops that can affect physiological performance and reduce crop yields. Abiotic stressors cause changes in protein synthesis, modifying the levels of a series of proteins, especially the heat shock proteins (HSP), and these proteins can help protect the plants against abiotic stress. The objective of this study was to verify if tomato plants cv. Micro-Tom from different genotypes with varying expression levels of MT-sHSP23.6 (mitochondrial small heat shock proteins) have different responses physiological to flooding. Plants from three genotypes (untransformed, MT-sHSP23.6 sense expression levels and MT-sHSP23.6 antisense expression levels) were cultivated under controlled conditions. After 50 days, the plants were flooded for 14 days. After this period half of the plants from each genotype were allowed to recover. Chlorophyll fluorescence, gas exchange, chlorophyll index, leaf area and dry matter were evaluated. Flood stress affected the photosynthetic electron transport chain, which is related to inactivation of the oxygen-evolving complex, loss of connectivity among units in photosystem II, oxidation-reduction of the plastoquinone pool and activity of photosystem I. The genotype with MT-sHSP23.6 sense expression levels was less sensitive to stress from flooding.
authors.affiliation: Programa de Pós-graduação em Fisiologia Vegetal - PPGFV, Departamento de Botânica - DB, Instituto de Biologia - IB, Universidade Federal de Pelotas - UFPel, Campus Capão do Leão, CEP 96010-900, Pelotas, RS, Brazil.
authors.forename: C M
authors.initials: CM
authors.lastname: Hüther
authors.affiliation: Programa de Pós-graduação em Fisiologia Vegetal - PPGFV, Departamento de Botânica - DB, Instituto de Biologia - IB, Universidade Federal de Pelotas - UFPel, Campus Capão do Leão, CEP 96010-900, Pelotas, RS, Brazil.|Laboratório de Metabolismo Vegetal, Departamento de Botânica - DB, Instituto de Biologia - IB, Universidade Federal de Pelotas - UFPel, Campus Capão do Leão, CEP 96010-900, Pelotas, RS, Brazil.
authors.forename: E G
authors.initials: EG
authors.lastname: Martinazzo
authors.affiliation: Departamento de Ciência e Tecnologia de Alimentos - DCTA, Faculdade de Agronomia Eliseu Maciel - FAEM, Universidade Federal de Pelotas - UFPel, Campus Capão do Leão, CEP 96010-900, Pelotas, RS, Brazil.
authors.forename: C V
authors.initials: CV
authors.lastname: Rombaldi
authors.affiliation: Programa de Pós-graduação em Fisiologia Vegetal - PPGFV, Departamento de Botânica - DB, Instituto de Biologia - IB, Universidade Federal de Pelotas - UFPel, Campus Capão do Leão, CEP 96010-900, Pelotas, RS, Brazil.|Laboratório de Metabolismo Vegetal, Departamento de Botânica - DB, Instituto de Biologia - IB, Universidade Federal de Pelotas - UFPel, Campus Capão do Leão, CEP 96010-900, Pelotas, RS, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Bacarin
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D055868:Floods; D005838:Genotype; D050888:Heat-Shock Proteins, Small / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000502:physiology*; D008928:Mitochondria / Q000378:metabolism*; D010788:Photosynthesis / Q000502:physiology; D045331:Photosystem I Protein Complex / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D050888:Heat-Shock Proteins, Small; D045331:Photosystem I Protein Complex; D002734:Chlorophyll