title: Ultrasensitive Glutathione Detection Based on Lucigenin Cathodic Electrochemiluminescence in the Presence of MnO2 Nanosheets.
abstract: Glutathione (GSH) is a crucial antioxidant produced endogenously and plays key roles in biological systems. It is vitally important to design simple, selective, and sensitive methods to sense GSH and monitor changes of GSH concentration. In this work, the cathodic electrochemiluminescence (ECL) of lucigenin in the presence of MnO2 nanosheets at a glassy carbon electrode was utilized for GSH detection. GSH can reduce MnO2 nanosheets into Mn(2+) which can obviously inhibit the ECL of lucigenin. The ECL inhibition efficiencies increase linearly with the concentrations of glutathione in the range of 10 to 2000 nM. The detection limit for GSH measurement is 3.7 nM. This proposed method is highly sensitive, selective, simple, fast, and cost-effective. Moreover, this approach can detect GSH in human serum samples with excellent recoveries, which indicates its promising application under physiological conditions.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, P.R. China.|University of Chinese Academy of Sciences , Beijing 100049, P.R. China.
authors.forename: Wenyue
authors.initials: W
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, P.R. China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, P.R. China.|University of Chinese Academy of Sciences , Beijing 100049, P.R. China.
authors.forename: Liming
authors.initials: L
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, P.R. China.|University of Chinese Academy of Sciences , Beijing 100049, P.R. China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Lai
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, P.R. China.|University of Chinese Academy of Sciences , Beijing 100049, P.R. China.
authors.forename: Shimeles Addisu
authors.initials: SA
authors.lastname: Kitte
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, P.R. China.
authors.forename: Guobao
authors.initials: G
authors.lastname: Xu