title: Oxygen-Evolving Porous Glass Plates Containing the Photosynthetic Photosystem II Pigment-Protein Complex.
abstract: The development of artificial photosynthesis has focused on the efficient coupling of reaction at photoanode and cathode, wherein the production of hydrogen (or energy carriers) is coupled to the electrons derived from water-splitting reactions. The natural photosystem II (PSII) complex splits water efficiently using light energy. The PSII complex is a large pigment-protein complex (20 nm in diameter) containing a manganese cluster. A new photoanodic device was constructed incorporating stable PSII purified from a cyanobacterium Thermosynechococcus vulcanus through immobilization within 20 or 50 nm nanopores contained in porous glass plates (PGPs). PSII in the nanopores retained its native structure and high photoinduced water splitting activity. The photocatalytic rate (turnover frequency) of PSII in PGP was enhanced 11-fold compared to that in solution, yielding a rate of 50-300 mol e(-)/(mol PSII·s) with 2,6-dichloroindophenol (DCIP) as an electron acceptor. The PGP system realized high local concentrations of PSII and DCIP to enhance the collisional reactions in nanotubes with low disturbance of light penetration. The system allows direct visualization/determination of the reaction inside the nanotubes, which contributes to optimize the local reaction condition. The PSII/PGP device will substantively contribute to the construction of artificial photosynthesis using water as the ultimate electron source.
authors.affiliation: The OCU Advanced Research Institute for Natural Science & Technology (OCARINA), Osaka City University , 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Noji
authors.affiliation: The OCU Advanced Research Institute for Natural Science & Technology (OCARINA), Osaka City University , 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kawakami
authors.affiliation: Photosynthesis Research Center, Graduate School of Natural Science and Technology/Faculty of Science, Okayama University , Okayama 700-8530, Japan.
authors.forename: Jian-Ren
authors.initials: JR
authors.lastname: Shen
authors.affiliation: Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology , Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan.
authors.forename: Takehisa
authors.initials: T
authors.lastname: Dewa
authors.affiliation: The OCU Advanced Research Institute for Natural Science & Technology (OCARINA), Osaka City University , 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Nango
authors.affiliation: The OCU Advanced Research Institute for Natural Science & Technology (OCARINA), Osaka City University , 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kamiya
authors.affiliation: Graduate School of Science, Nagoya University , Furo-cho, Chikusa-ku Nagoya, Aichi 464-8602, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Itoh
authors.affiliation: Inorganic Functional Materials Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) , 1-8-31, Midorigaoka, Ikeda, Osaka 563-8577, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Jin
mesh_terms: D015086:2,6-Dichloroindophenol / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D000458:Cyanobacteria / Q000201:enzymology*; D005898:Glass / Q000737:chemistry*; D058608:Nanopores; D010100:Oxygen / Q000737:chemistry*; D045332:Photosystem II Protein Complex / Q000737:chemistry*; D016062:Porosity
chemical_list: D001426:Bacterial Proteins; D045332:Photosystem II Protein Complex; D015086:2,6-Dichloroindophenol; D010100:Oxygen