title: Effects of Synthetic Serum Supplementation in Sperm Preparation Media on Sperm Capacitation and Function Test Results.
abstract: Albumin supplementation of culture media induces sperm capacitation in assisted reproduction technique cycles. Synthetic serum supplementation is clinically used to replace albumin for preventing transmission of infectious agents. However, the effects of synthetic serum supplementation on sperm capacitation have rarely been investigated. Spermatozoa from 30 men with normal basic semen analysis results were collected, divided into five aliquots, and cultured in capacitating conditions in four combinations of two synthetic serum supplements, serum substitute supplement (SSS) and serum protein substitute (SPS), and two fertilization media, Quinns Advantage™ Fertilization (QF) and human tubular fluid (HTF) media. Reactive oxygen species (ROS) levels in spermatozoa were measured through chemiluminescence. Furthermore, acrosome reaction and western blotting for tyrosine phosphorylation were used to evaluate sperm capacitation. HTF+SSS had significantly higher ROS levels than QF+SPS did (11,725 ± 1,172 versus 6,278 ± 864 relative light units). In addition, the spermatozoa cultured in QF+SPS had lower motility, acrosome reaction rates, and tyrosine phosphorylation levels compared with those cultured in HTF+SSS. In conclusion, the effects of synthetic serum supplementation on sperm capacitation varied according to the combination of media. These differences may lead to variations in spermatozoon ROS levels, thus affecting sperm function test results.
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan; Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung 402, Taiwan.
authors.forename: Ying-Fu
authors.initials: YF
authors.lastname: Shih
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Tzeng
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan; Department of Urology, Chung Shan Medical University Hospital, Taichung 402, Taiwan.
authors.forename: Wen-Jung
authors.identifier: 0000-0002-4771-8074
authors.initials: WJ
authors.lastname: Chen
authors.affiliation: Division of Infertility Clinic, Lee Women's Hospital, Taichung 406, Taiwan.
authors.forename: Chun-Chia
authors.initials: CC
authors.lastname: Huang
authors.affiliation: Division of Infertility Clinic, Lee Women's Hospital, Taichung 406, Taiwan.
authors.forename: Hsiu-Hui
authors.identifier: 0000-0002-5386-5775
authors.initials: HH
authors.lastname: Chen
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan; Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung 402, Taiwan; Department of Obstetrics and Gynecology, National Taiwan University and National Taiwan University Hospital, Taipei 100, Taiwan.
authors.forename: Tsung-Hsien
authors.identifier: 0000-0001-8858-0916
authors.initials: TH
authors.lastname: Lee
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan; Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung 402, Taiwan; Division of Infertility Clinic, Lee Women's Hospital, Taichung 406, Taiwan.
authors.forename: Maw-Sheng
authors.identifier: 0000-0001-6234-5432
authors.initials: MS
authors.lastname: Lee
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects; D000328:Adult; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D008297:Male; D017382:Reactive Oxygen Species / Q000378:metabolism; D044967:Serum / Q000187:drug effects / Q000378:metabolism*; D012709:Serum Albumin / Q000494:pharmacology*; D013075:Sperm Capacitation / Q000187:drug effects*; D013081:Sperm Motility / Q000187:drug effects
chemical_list: D017382:Reactive Oxygen Species; D012709:Serum Albumin