title: Multiplexed longitudinal measurement of protein biomarkers in DBS using an automated SISCAPA workflow.
abstract: BACKGROUND
  The use of DBS for quantitative protein biomarker measurement has been hindered by issues associated with blood hematocrit variations and lack of detection sensitivity, particularly when multiple biomarkers are measured.
  
  
  METHODS
  An automated, multiplexed SISCAPA analysis was used to normalize blood volume variations in DBS and quantify proteins of varying abundance in longitudinal specimens.
  
  
  CONCLUSIONS
  The results showed that after normalizing the spot-to-spot hematocrit variations, peptide surrogates of protein biomarkers could be accurately quantitated in DBS. This allowed the establishment of baselines for a variety of biomarkers in multiple individuals and enabled detection of changes over time, thus offering an effective solution for longitudinal personal monitoring of biomarkers relevant in health and disease.
authors.affiliation: SISCAPA Assay Technologies, Inc., Box 53309, Washington, DC 20009, USA.
authors.forename: Morteza
authors.initials: M
authors.lastname: Razavi
authors.affiliation: SISCAPA Assay Technologies, Inc., Box 53309, Washington, DC 20009, USA.
authors.forename: N Leigh
authors.initials: NL
authors.lastname: Anderson
authors.affiliation: SISCAPA Assay Technologies, Inc., Box 53309, Washington, DC 20009, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Yip
authors.affiliation: SISCAPA Assay Technologies, Inc., Box 53309, Washington, DC 20009, USA.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Pope
authors.affiliation: SISCAPA Assay Technologies, Inc., Box 53309, Washington, DC 20009, USA.
authors.forename: Terry W
authors.initials: TW
authors.lastname: Pearson
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D001810:Blood Volume; D002853:Chromatography, Liquid / Q000379:methods; D059788:Dried Blood Spot Testing / Q000379:methods*; D006400:Hematocrit; D006801:Humans; D053719:Tandem Mass Spectrometry / Q000379:methods; D057188:Workflow
chemical_list: D015415:Biomarkers; D001798:Blood Proteins
keywords: DBS; MS; SISCAPA; longitudinal; monoclonal antibodies; protein biomarkers