title: Biological conversion of gaseous alkenes to liquid chemicals.
abstract: Industrial gas-to-liquid (GTL) technologies are well developed. They generally employ syngas, require complex infrastructure, and need high capital investment to be economically viable. Alternatively, biological conversion has the potential to be more efficient, and easily deployed to remote areas on relatively small scales for the utilization of otherwise stranded resources. The present study demonstrates a novel biological GTL process in which engineered Escherichia coli converts C2-C4 gaseous alkenes into liquid diols. Diols are versatile industrially important chemicals, used routinely as antifreeze agents, polymer precursors amongst many other applications. Heterologous co-expression of a monooxygenase and an epoxide hydrolase in E. coli allows whole cell conversion of C2-C4 alkenes for the formation of ethylene glycol, 1,2-propanediol, 1,2-butanediol, and 2,3-butanediol at ambient temperature and pressure in one pot. Increasing intracellular NADH supply via addition of formate and a formate dehydrogenase increases ethylene glycol production titers, resulting in an improved productivity of 9mg/L/h and a final titer of 250mg/L. This represents a novel biological method for GTL conversion of alkenes to industrially valuable diols.
authors.affiliation: Department of Chemistry, University of California-Davis, Davis, CA, USA.
authors.forename: Shuchi H
authors.initials: SH
authors.lastname: Desai
authors.affiliation: Department of Chemistry, University of California-Davis, Davis, CA, USA.
authors.forename: Irina
authors.initials: I
authors.lastname: Koryakina
authors.affiliation: Department of Chemistry, University of California-Davis, Davis, CA, USA.
authors.forename: Anna E
authors.initials: AE
authors.lastname: Case
authors.affiliation: Department of Chemistry, University of California-Davis, Davis, CA, USA. Electronic address: mdtoney@ucdavis.edu.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Toney
authors.affiliation: Department of Chemistry, University of California-Davis, Davis, CA, USA. Electronic address: satsumi@ucdavis.edu.
authors.forename: Shota
authors.initials: S
authors.lastname: Atsumi
mesh_terms: D000438:Alcohols / Q000302:isolation & purification / Q000378:metabolism*; D000475:Alkenes / Q000378:metabolism*; D004851:Epoxide Hydrolases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005740:Gases / Q000378:metabolism*; D024861:Genetic Enhancement; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism; D044367:Phase Transition; D012996:Solutions / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000438:Alcohols; D000475:Alkenes; D029968:Escherichia coli Proteins; D005740:Gases; D012996:Solutions; D006899:Mixed Function Oxygenases; D004851:Epoxide Hydrolases
keywords: Alkenes; Biological gas to liquid conversion; Diols; Metabolic engineering