title: Modification and cytocompatibility of biocomposited porous PLLA/HA-microspheres scaffolds.
abstract: Poly(L-lactic acid) and hydroxyapatie (PLLA/HA) composite scaffolds have good properties and suit to use as bone tissue engineering. In this work, hollow HA microspheres (HAM) with poor crystallinity were fabricated by a flame-drying method. The HAM has the potential to be used to release drugs or proteins in addition to improve osteoconductivity. Different ratios of PLLA/HAM were used to prepare porous composite scaffolds using the thermally induced phase separation technique. The HAMs were randomly incorporated into the PLLA porous scaffolds. As the HAMs ratio was increased, the porous composite scaffolds changed from ladder-like into isotropic structure. In addition, the compressive strength of PLLA/HAMs composite scaffolds improved first and declined with the increasing of HAMs ratio in the scaffolds. In vitro experiment showed that PLLA/HAMs composite scaffolds improved the attachment, migration, and differentiation of osteoblastic cells. These results demonstrated that the PLLA/HAMs composite scaffolds were superior to plain PLLA scaffold for bone tissue engineering.
authors.affiliation: a Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education , Shandong University , Ji'nan , PR China.|b School of Materials Science and Engineering , Shandong University , Ji'nan , PR China.|c Suzhou Institute of Shandong University , Shandong University , Suzhou , PR China.
authors.forename: Guiyong
authors.initials: G
authors.lastname: Xiao
authors.affiliation: d Department of Orthopaedics , The People's Hospital of Liaocheng , Liaocheng , PR China.
authors.forename: Han
authors.initials: H
authors.lastname: Yin
authors.affiliation: a Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education , Shandong University , Ji'nan , PR China.|b School of Materials Science and Engineering , Shandong University , Ji'nan , PR China.|c Suzhou Institute of Shandong University , Shandong University , Suzhou , PR China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Xu
authors.affiliation: a Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education , Shandong University , Ji'nan , PR China.|b School of Materials Science and Engineering , Shandong University , Ji'nan , PR China.|c Suzhou Institute of Shandong University , Shandong University , Suzhou , PR China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Lu
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008863:Microspheres; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C499699:hydroxyapatite-polylactide; C033616:poly(lactide); D017886:Durapatite
keywords: Hydroxyapatite; microspheres; osteoblast; scaffold; tissue engineering