title: BRCA1 and BRCA2 sequence variations detected with next-generation sequencing in patients with premature ovarian insufficiency.
abstract: OBJECTIVE
  Although the association between BRCA1 and BRCA2 gene mutations and breast and ovarian cancer is known, there is insufficient data about premature ovarian insufficiency (POI). However, several studies have reported that there might be a relationship between POI and BRCA1 and BRCA2 gene mutation. Therefore, in the present study, we aimed to investigate the role of BRCA1 and BRCA2 gene mutations in the etiology of POI in a Turkish population.
  
  
  METHODS
  The cohort was classified into two groups: a study group, consisting of 56 individuals diagnosed with premature ovarian insufficiency (and who were younger than 40 years of age, had an antral follicle count <3-5, and FSH levels >12 IU/I), and a control group, consisting of 45 fertile individuals. A total of 101 individuals were analyzed by next-generation sequencing to detect BRCA1 and BRCA2 gene mutations.
  
  
  RESULTS
  We detected four new variations (p.T1246N and p.R1835Q in BRCA1 and p.I3312V and IVS-7T>A in BRCA2) that had not been reported before.
  
  
  CONCLUSIONS
  We did not find an association between the BRCA1 and BRCA2 gene mutations and premature ovarian insufficiency. However, larger, functional studies are needed to clarify the association.
authors.affiliation: Department of Reproductive Endocrinology, Zekai Tahir Burak Women's Health Training and Research Hospital, Ankara, Turkey.
authors.forename: Nafiye Karakaş
authors.initials: NK
authors.lastname: Yılmaz
authors.affiliation: Department of Medical Genetics, Başkent University School of Medicine, Ankara, Turkey.
authors.forename: Peren Hatice
authors.initials: PH
authors.lastname: Karagin
authors.affiliation: Department of Medical Genetics, Başkent University School of Medicine, Ankara, Turkey.
authors.forename: Yunus Kasım
authors.initials: YK
authors.lastname: Terzi
authors.affiliation: Department of Reproductive Endocrinology, Zekai Tahir Burak Women's Health Training and Research Hospital, Ankara, Turkey.
authors.forename: İnci
authors.initials: İ
authors.lastname: Kahyaoğlu
authors.affiliation: Department of Reproductive Endocrinology, Zekai Tahir Burak Women's Health Training and Research Hospital, Ankara, Turkey.
authors.forename: Saynur
authors.initials: S
authors.lastname: Yılmaz
authors.affiliation: Department of Reproductive Endocrinology, Zekai Tahir Burak Women's Health Training and Research Hospital, Ankara, Turkey.
authors.forename: Salim
authors.initials: S
authors.lastname: Erkaya
authors.affiliation: Department of Medical Genetics, Başkent University School of Medicine, Ankara, Turkey.
authors.forename: Feride İffet
authors.initials: Fİ
authors.lastname: Şahin
keywords: BRCA1; BRCA2; Premature ovarian insufficiency; in vitro fertilization; next generation sequencing