title: A roadmap for biocatalysis - functional and spatial orchestration of enzyme cascades.
abstract: Advances in biological engineering and systems biology have provided new approaches and tools for the industrialization of biology. In the next decade, advanced biocatalytic systems will increasingly be used for the production of chemicals that cannot be made by current processes and/or where the use of enzyme catalysts is more resource efficient with a much reduced environmental impact. We expect that in the future, manufacture of chemicals and materials will utilize both biocatalytic and chemical synthesis synergistically. The realization of such advanced biomanufacturing processes currently faces a number of major challenges. Ready-to-deploy portfolios of biocatalysts for design to production must be created from biological diverse sources and through protein engineering. Robust and efficient multi-step enzymatic reaction cascades must be developed that can operate simultaneously in one-pot. For this to happen, bio-orthogonal strategies for spatial and temporal control of biocatalyst activities must be developed. Promising approaches and technologies are emerging that will eventually lead to the design of in vitro biocatalytic systems that mimic the metabolic pathways and networks of cellular systems which will be discussed in this roadmap.
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 140 Gortner Laboratory, 1479 Gortner Avenue, St. Paul, MN, 55108, USA.
authors.forename: Claudia
authors.initials: C
authors.lastname: Schmidt-Dannert
authors.affiliation: Heterogeneous Biocatalysis Group, CIC BiomaGUNE, Pase Miramon 182, San Sebastian-Donostia, Spain.|Ikerbasque, Basque Foundation for Science, Bilbao, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Lopez-Gallego
mesh_terms: D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods*; D004798:Enzymes / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D009930:Organic Chemicals / Q000138:chemical synthesis / Q000378:metabolism*
chemical_list: D004798:Enzymes; D009930:Organic Chemicals