title: Antioxidant activity and chemical composition of Juniperus excelsa ssp. polycarpos wood extracts.
abstract: Extracts from the wood of Juniperus excelsa ssp. polycarpos were analysed for their antioxidant activity using the DPPH method and compared with ascorbic acid and butylated hydroxytoluene. The most active extracts were analysed for their chemical composition using gas chromatography-mass spectrometry. Acetone extract was found to be moderately active as an antioxidant agent at 58.38%, which was lower than the value of vitamin C (98.56%) at the concentration of 14.20 mg/mL. The major components identified in the acetone extract as trimethylsilyl (TMS) derivatives were pimaric acid TMS (24.56%), followed by α-d-glucopyranoside,1,3,4,6-tetrakis-O-(TMS)-β-d-fructofuranosyl 2,3,4,6-tetrakis-O-(TMS) (21.39%), triflouromethyl-bis-(TMS)methyl ketone (9.32%), and cedrol (0.72%). The dissolved water:methanol (1:1 v/v) partitioned from acetone extract afforded 12 fractions; among them, the F9 fraction was found to have good antioxidant activity (88.49%) at the concentration of 14.20 mg/mL. The major compounds identified in F9 fraction were α-d-glucopyranoside, 1,3,4,6-tetrakis-O-(TMS) (20.22%) and trifluoromethyl-bis-(TMS)methyl ketone (5.10%).
authors.affiliation: a Department of Wood Science and Paper Technology, Karaj Branch , Islamic Azad University , Karaj , Iran.
authors.forename: S K
authors.initials: SK
authors.lastname: Hosseinihashemi
authors.affiliation: a Department of Wood Science and Paper Technology, Karaj Branch , Islamic Azad University , Karaj , Iran.
authors.forename: A
authors.initials: A
authors.lastname: Dadpour
authors.affiliation: a Department of Wood Science and Paper Technology, Karaj Branch , Islamic Azad University , Karaj , Iran.
authors.forename: A
authors.initials: A
authors.lastname: Lashgari
mesh_terms: D000096:Acetone / Q000737:chemistry; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D018653:Juniperus / Q000737:chemistry*; D000432:Methanol; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D012997:Solvents; D014867:Water; D014934:Wood / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D000096:Acetone; D002084:Butylated Hydroxytoluene; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid; D000432:Methanol
keywords: GC/MS; Juniperus excelsa ssp. polycarpos wood; acetone extract; antioxidant activity; fraction