title: Manufacturing High Purity Maltose and Maltotetraose from Starch by a Novel and Efficient Procedure Named "Reducing End Modification Method".
abstract: A novel and efficient procedure named "reducing end modification method" was developed for manufacturing high purity maltooligosaccharides. By the method, high purity maltose and maltotetraose were prepared from starch. Starch was liquefied, debranched, and oxidized with sodium hypochlorite at the reducing end. The reaction mixture was treated with β-amylase or maltotetraose-forming amylase from Pseudomonas stutzeri IFO-3773, resulting in the production of high purity maltose or maltotetraose, oxidized maltooligosaccharides, and oxidized undigested dextrin. High purity maltose (maltose content, 99.9%) or maltotetraose (maltotetraose content, 95%) was obtained very effectively from the reaction mixture by chromatography on a strong acid cation exchange resin (Na (+)).
authors.affiliation: a Discovery Research Laboratory III, Nippon Shinyaku Co., Ltd. , Nishioji-Hachijo, Minami-ku, Kyoto 601 , Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ezure
authors.affiliation: a Discovery Research Laboratory III, Nippon Shinyaku Co., Ltd. , Nishioji-Hachijo, Minami-ku, Kyoto 601 , Japan.
authors.forename: S
authors.initials: S
authors.lastname: Maruo
authors.affiliation: a Discovery Research Laboratory III, Nippon Shinyaku Co., Ltd. , Nishioji-Hachijo, Minami-ku, Kyoto 601 , Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kojima
authors.affiliation: a Discovery Research Laboratory III, Nippon Shinyaku Co., Ltd. , Nishioji-Hachijo, Minami-ku, Kyoto 601 , Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sakai
authors.affiliation: a Discovery Research Laboratory III, Nippon Shinyaku Co., Ltd. , Nishioji-Hachijo, Minami-ku, Kyoto 601 , Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.affiliation: a Discovery Research Laboratory III, Nippon Shinyaku Co., Ltd. , Nishioji-Hachijo, Minami-ku, Kyoto 601 , Japan.
authors.forename: N
authors.initials: N
authors.lastname: Tachikake
authors.affiliation: a Discovery Research Laboratory III, Nippon Shinyaku Co., Ltd. , Nishioji-Hachijo, Minami-ku, Kyoto 601 , Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.affiliation: a Discovery Research Laboratory III, Nippon Shinyaku Co., Ltd. , Nishioji-Hachijo, Minami-ku, Kyoto 601 , Japan.
authors.forename: M
authors.initials: M
authors.lastname: Toda
keywords: maltose; maltotetraose; maltotetraose-forming amylase; reducing end modification method; β-amylase