title: Anti-inflammatory and angiogenic activity of polysaccharide extract obtained from Tibetan kefir.
abstract: The search for new bioactive molecules is a driving force for research pharmaceutical industries, especially those molecules obtained from fermentation. The molecules possessing angiogenic and anti-inflammatory attributes have attracted attention and are the focus of this study. Angiogenic activity from kefir polysaccharide extract, via chorioallantoic membrane assay, exhibited a pro-angiogenic effect compared with vascular endothelial factor (pro-angiogenic) and hydrocortisone (anti-angiogenic) activity as standards with an EC50 of 192ng/mL. In terms of anti-inflammatory activity determined via hyaluronidase enzyme assay, kefir polysaccharide extract inhibited the enzyme with a minimal activity of 2.08mg/mL and a maximum activity of 2.57mg/mL. For pharmaceutical purposes, kefir polysaccharide extract is considered to be safe because it does not inhibit VERO cells in cytotoxicity assays.
authors.affiliation: Faculdades Pequeno Príncipe, Curitiba, PR, Brazil. Electronic address: mrosaprado@hotmail.com.
authors.forename: Maria Rosa Machado
authors.initials: MR
authors.lastname: Prado
authors.affiliation: Faculdades Pequeno Príncipe, Curitiba, PR, Brazil.
authors.forename: Christian
authors.initials: C
authors.lastname: Boller
authors.affiliation: Faculdades Pequeno Príncipe, Curitiba, PR, Brazil; Instituto de Pesquisa Pelé Pequeno Príncipe de Pesquisa, Curitiba, PR, Brazil.
authors.forename: Rosiane Guetter Mello
authors.initials: RG
authors.lastname: Zibetti
authors.affiliation: Instituto de Pesquisa Pelé Pequeno Príncipe de Pesquisa, Curitiba, PR, Brazil.
authors.forename: Daiany
authors.initials: D
authors.lastname: de Souza
authors.affiliation: Instituto de Tecnologia do Paraná, Paraná, Curitiba, PR, Brazil.
authors.forename: Luciana Lopes
authors.initials: LL
authors.lastname: Pedroso
authors.affiliation: Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Carlos Ricardo
authors.initials: CR
authors.lastname: Soccol
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo; D002645:Chickens; D002522:Chlorocebus aethiops; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D004305:Dose-Response Relationship, Drug; D065089:Glycoside Hydrolase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000070716:Kefir / Q000382:microbiology*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D014709:Vero Cells
chemical_list: D043925:Angiogenesis Inducing Agents; D000893:Anti-Inflammatory Agents; D065089:Glycoside Hydrolase Inhibitors; D011135:Polysaccharides, Bacterial; D006821:Hyaluronoglucosaminidase
keywords: Angiogenic activity; Anti-inflammatory activity; Hyaluronidase; Tibetan kefir