title: AZDAST the new horizon in antimicrobial synergism detection.
abstract: The attempts via introducing many methods have been conducted to select the best antibiotic combination in the treatment of seriously ill patients. Operational or interpretational complexity or time-consuming along with sufficient accuracy led to postpone routine clinical use of these tests until today, despite the urgent need for them. By this study and proposed method, selection of the best double antibiotic synergistic combination against resistant pathogen is simply same as Kirby-Bauer antibiotic susceptibility test. It seems, precise and reliable results (very low coefficient of variation) will be introduced it as a routine accurate diagnostic doubled antimicrobial synergism test.•The objective of this study was to introduce a novel method in antibiotic interaction detection.•It demonstrates high sensitivity and accuracy.•Easy implementation by routine microbiology labs materials and equipment and so easy stand-alone interpretation seems to make it friendly test be able to replacing the previous methods.
authors.affiliation: Department of Veterinary Medicine, Faculty of Agriculture, Islamic Azad University, Yasuj Branch, Yasuj, Iran.
authors.forename: Navid
authors.initials: N
authors.lastname: Ziaei-Darounkalaei
authors.affiliation: Department of Pathology, Amgen Inc., USA.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Ameri
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Taghi
authors.initials: T
authors.lastname: Zahraei-Salehi
authors.affiliation: Department of Orthopedic Surgery, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Omid
authors.initials: O
authors.lastname: Ziaei-Darounkalaei
authors.affiliation: Department of General Surgery, Gorgan University of Medical Sciences, Gorgan, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Mohajer-Tabrizi
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Islamic Azad University, Yasuj Branch, Yasuj, Iran.
authors.forename: Lotfollah
authors.initials: L
authors.lastname: Bornaei
keywords: AZDAST antibiotic double combination