title: Determination and Pharmacokinetic Study of Pirfenidone in Rat Serum by High-Performance Thin-Layer Chromatography.
abstract: A rapid, sensitive and selective high-performance thin-layer chromatography (HPTLC) method was developed and validated for the determination and pharmacokinetics of pirfenidone in rat serum. One-step protein precipitation by methanol is reported, and serum samples were separated by HPTLC using a simple mobile phase of toluene-methanol in the ratio of 8:2. The retardation factor of pirfenidone in the serum sample was 0.45 with the detection performed at 315 nm. The calibration curve was linear over the range of 100-1,200 ng/spot with a lower limit of quantitation of 40 ng/spot. The mean recovery of pirfenidone in serum was in the range of 70.6-75.8%, and intra-day and inter-day precision were both <14.1%. This method was successfully applied to the pharmacokinetic study of pirfenidone in rats on oral administration of the drug at a dose of 15.0 mg/kg.
authors.affiliation: Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur 440 033, MS, India.
authors.forename: Sonali G
authors.initials: SG
authors.lastname: Thorat
authors.affiliation: Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur 440 033, MS, India Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Mumbai 410 210, MS, India rupeshchikhale7@gmail.com.
authors.forename: Rupesh V
authors.initials: RV
authors.lastname: Chikhale
mesh_terms: D000096:Acetone; D000284:Administration, Oral; D000700:Analgesics / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D002138:Calibration; D002855:Chromatography, Thin Layer / Q000592:standards*; D003720:Densitometry / Q000592:standards*; D057230:Limit of Detection; D008297:Male; D011728:Pyridones / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D014050:Toluene
chemical_list: D000700:Analgesics; D011728:Pyridones; D000096:Acetone; D014050:Toluene; C093844:pirfenidone