title: Role of vitamin D3 in regulation of interleukin-6 and osteopontin expression in liver of diabetic mice.
abstract: To study the link between hepatic interleukin-6 (IL-6) and osteopontin (OPN) gene expression and vitamin D3 status associated with type 1 diabetes in mice; and to evaluate the effects of vitamin D3 treatment (800 IU/kg of body weight for 6 weeks) on diabetes-induced impairments.
  
  
  
  mRNA levels of IL-6 and OPN were measured by quantitative RT-PCR. Blood serum 25OHD3 was assayed by ELISA.
  
  
  
  It was shown that induction of IL-6 in diabetic liver is accompanied by increased expression of OPN. Changes in OPN and IL-6 RNA levels correlated with a lack of 25OHD3 in serum. Vitamin D3 treatment restored 25OHD3 that led to a substantial reduction of OPN and IL-6 mRNA levels.
  
  
  
  Diabetes-induced vitamin D3 deficiency was associated with increased hepatic levels of IL-6 and OPN mRNA and these changes were countered by vitamin D3 administration.
authors.affiliation: Laboratory of Medical Biochemistry, O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
authors.forename: D
authors.initials: D
authors.lastname: Labudzynskyi
authors.forename: I
authors.initials: I
authors.lastname: Shymanskyy
authors.forename: M
authors.initials: M
authors.lastname: Veliky
mesh_terms: D000818:Animals; D002762:Cholecalciferol / Q000378:metabolism* / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D053495:Osteopontin; D002762:Cholecalciferol