title: Single Walled Carbon Nanotube-Based Electrical Biosensor for the Label-Free Detection of Pathogenic Bacteria.
abstract: We herein describe the development of a single-walled carbon nanotube (SWNT)-based electrical biosensor consisting of a two-terminal resistor, and report its use for the specific, label-free detection of pathogenic bacteria via changes in conductance. The ability of this biosensor to recognize different pathogenic bacteria was analyzed, and conditions were optimized with different probe concentrations. Using this system, the reference strains and clinical isolates of Staphylococcus aureus and Escherichia coli were successfully detected; in both cases, the sensor showed a detection limit of 10 CFU. This SWNT-based electrical biosensor will prove useful for the development of highly sensitive and specific handheld pathogen detectors.
authors.forename: Seung Min
authors.initials: SM
authors.lastname: Yoo
authors.forename: Youn-Kyoung
authors.initials: YK
authors.lastname: Baek
authors.forename: SunHaeRa
authors.initials: S
authors.lastname: Shin
authors.forename: Ju-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Hee-Tae
authors.initials: HT
authors.lastname: Jung
authors.forename: Yang-Kyu
authors.initials: YK
authors.lastname: Choi
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004563:Electrochemistry; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D057230:Limit of Detection; D037742:Nanotubes, Carbon / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D037742:Nanotubes, Carbon