title: Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis.
abstract: This article reviews recent developments in droplet microfluidics enabling high-throughput single-cell analysis. Five key aspects in this field are included in this review: (1) prototype demonstration of single-cell encapsulation in microfluidic droplets; (2) technical improvements of single-cell encapsulation in microfluidic droplets; (3) microfluidic droplets enabling single-cell proteomic analysis; (4) microfluidic droplets enabling single-cell genomic analysis; and (5) integrated microfluidic droplet systems enabling single-cell screening. We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on key performances of throughput, multifunctionality, and absolute quantification.
authors.affiliation: Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. wenna14@mails.ucas.ac.cn.
authors.forename: Na
authors.initials: N
authors.lastname: Wen
authors.affiliation: Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. zhaozhan@mail.ie.ac.cn.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. fanbeiyuan@ucas.ac.cn.
authors.forename: Beiyuan
authors.initials: B
authors.lastname: Fan
authors.affiliation: Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. dychen@mail.ie.ac.cn.
authors.forename: Deyong
authors.initials: D
authors.lastname: Chen
authors.affiliation: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. d.men@wh.iov.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Men
authors.affiliation: Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. jbwang@mail.ie.ac.cn.
authors.forename: Junbo
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. chenjian@mail.ie.ac.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D023281:Genomics / Q000295:instrumentation / Q000379:methods; D057166:High-Throughput Screening Assays; D006801:Humans; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*
keywords: droplet microfluidics; high-throughput; single-cell encapsulation; single-cell genetic analysis; single-cell proteomic analysis; single-cell screening