title: High FABP5 Versus CRABPII Expression Ratio in Recurrent Craniopharyngiomas: Implications for Future Treatment.
abstract: OBJECTIVE
  Recurrence is a major problem in craniopharyngioma (CP) management. Recent study shows that high FABP5/CRABPII may be related to tumor growth and that all-trans retinoic acid (ATRA) may suppress primary CP growth. We studied the expression profile of FABP5 and CRABPII in recurrent CP tissue and the effect of ATRA on recurrent CP cells.
  
  
  METHODS
  Fifty cases of patients with CP were enrolled in the retrospective study. Among them, 15 were recurrent. Fresh specimens were collected for immunohistochemistry, reverse transcription polymerase chain reaction, and western blotting analysis of FABP5 and CRABPII. Fresh specimens from 6 primary and recurrent CPs were collected and subjected to cell culture using an explants method. ATRA at various concentrations was applied to recurrent CP cell culture, and cell growth was recorded and analyzed.
  
  
  RESULTS
  Immunohistochemistry, reverse transcription polymerase chain reaction, and western blot study showed that FABP5 was expressed significantly higher in recurrent tumors, whereas CRABPII was expressed significantly higher in primary tumors. The FABP5/CRABPII ratio was significantly higher in recurrent rather than primary tumors. Recurrent CP cells grew faster than primary cells, and ATRA induced cellular apoptosis and inhibited CP cell growth in a dose-dependent manner.
  
  
  CONCLUSIONS
  A high expression ratio between FABP5 and CRABPII may be related to CP tumor recurrence and ATRA could be a potential therapeutic agent for CP chemotherapy.
authors.affiliation: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: You
authors.affiliation: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, People's Republic of China.
authors.forename: Liangxue
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, People's Republic of China.
authors.forename: Xiutian
authors.initials: X
authors.lastname: Sima
authors.affiliation: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, People's Republic of China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, People's Republic of China. Electronic address: jianguo1229@gmail.com.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003397:Craniopharyngioma / Q000175:diagnosis / Q000453:epidemiology / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis / Q000453:epidemiology / Q000378:metabolism*; D010911:Pituitary Neoplasms / Q000175:diagnosis / Q000453:epidemiology / Q000378:metabolism*; D011379:Prognosis; D018168:Receptors, Retinoic Acid / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C493412:FABP5 protein, human; D050556:Fatty Acid-Binding Proteins; D018168:Receptors, Retinoic Acid; C082928:retinoic acid binding protein II, cellular
keywords: Apoptosis; CRABPII; Craniopharyngioma; FABP5; Retinoic acid