title: Phenylboronic acid modified solid-phase extraction column: Preparation, characterization, and application to the analysis of amino acids in sepia capsule by removing the maltose.
abstract: Maltose, a common auxiliary material of pharmaceutical preparation, may disturb the analysis of total amino acids in sepia capsule by aldolization. Therefore, it is necessary to remove the maltose through a convenient method. In this work, a phenylboronic acid modified solid-phase extraction column has been synthesized and used to remove the maltose. The materials were synthesized by one step "thiol-ene" reaction and the parameters of the column such as absorption capacity, recovery, and absorption specificity have been investigated. The results showed the column (0.5 cm of length × 0.5 cm of inner diameter) can absorb 4.6 mg maltose with a linear absorption and absorption specificity. Then this technique was applied in the quantification of amino acids in sepia capsule. After the optimization of the method, four kinds of amino acids, which were the most abundant, were quantified by high-performance liquid chromatography with diode array detection. The amounts of the four kinds of amino acids are 1.5∼2 times more than that without the treatment of solid-phase extraction column, which almost overcomes the influence of the maltose. All the results indicate that the phenylboronic acid modified solid-phase extraction column can successfully help to accurately quantify the total amino acids in sepia capsule.
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.|College of pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Mengzhe
authors.initials: M
authors.lastname: Guo
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.|College of pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Dengyang
authors.initials: D
authors.lastname: Yin
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.|College of pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Han
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.|College of pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.|College of pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.|College of pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: He
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.|College of pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Du
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China. tdq993@hotmail.com.|College of pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China. tdq993@hotmail.com.
authors.forename: Daoquan
authors.initials: D
authors.lastname: Tang
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D002214:Capsules / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008320:Maltose / Q000032:analysis / Q000302:isolation & purification*; D049833:Sepia / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D000596:Amino Acids; D001897:Boronic Acids; D002214:Capsules; D008320:Maltose; C010686:benzeneboronic acid
keywords: Amino acids; Phenylboronic acid; Solid-phase extraction; Traditional Chinese medicine