title: Metaproteomic analysis of atmospheric aerosol samples.
abstract: Metaproteomic analysis of air particulate matter provides information about the abundance and properties of bioaerosols in the atmosphere and their influence on climate and public health. We developed and applied efficient methods for the extraction and analysis of proteins from glass fiber filter samples of total, coarse, and fine particulate matter. Size exclusion chromatography was applied to remove matrix components, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied for protein fractionation according to molecular size, followed by in-gel digestion and LC-MS/MS analysis of peptides using a hybrid Quadrupole-Orbitrap MS. Maxquant software and the Swiss-Prot database were used for protein identification. In samples collected at a suburban location in central Europe, we found proteins that originated mainly from plants, fungi, and bacteria, which constitute a major fraction of primary biological aerosol particles (PBAP) in the atmosphere. Allergenic proteins were found in coarse and fine particle samples, and indications for atmospheric degradation of proteins were observed. Graphical abstract Workflow for the metaproteomic analysis of atmospheric aerosol samples.
authors.affiliation: Department of Multiphase Chemistry, Max Planck Institute for Chemistry, Hahn-Meitner-Weg 1, 55128, Mainz, Germany.
authors.forename: Fobang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Multiphase Chemistry, Max Planck Institute for Chemistry, Hahn-Meitner-Weg 1, 55128, Mainz, Germany.|School of Environment and Energy, South China University of Technology, Higher Education Mega Center, Guangzhou, 510006, People's Republic of China.
authors.forename: Senchao
authors.initials: S
authors.lastname: Lai
authors.affiliation: Department of Multiphase Chemistry, Max Planck Institute for Chemistry, Hahn-Meitner-Weg 1, 55128, Mainz, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Reinmuth-Selzle
authors.affiliation: Department of Multiphase Chemistry, Max Planck Institute for Chemistry, Hahn-Meitner-Weg 1, 55128, Mainz, Germany.
authors.forename: Jan Frederik
authors.initials: JF
authors.lastname: Scheel
authors.affiliation: Department of Multiphase Chemistry, Max Planck Institute for Chemistry, Hahn-Meitner-Weg 1, 55128, Mainz, Germany.
authors.forename: Janine
authors.initials: J
authors.lastname: Fröhlich-Nowoisky
authors.affiliation: Institute of General Botany, Johannes Gutenberg University Mainz, Johannes-von-Müller-Weg 6, 55128, Mainz, Germany.
authors.forename: Viviane R
authors.initials: VR
authors.lastname: Després
authors.affiliation: Institute for Inorganic and Analytical Chemistry, Johannes Gutenberg University Mainz, Duesbergweg 10-14, 55128, Mainz, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Hoffmann
authors.affiliation: Department of Multiphase Chemistry, Max Planck Institute for Chemistry, Hahn-Meitner-Weg 1, 55128, Mainz, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Pöschl
authors.affiliation: Department of Multiphase Chemistry, Max Planck Institute for Chemistry, Hahn-Meitner-Weg 1, 55128, Mainz, Germany. c.kampf@mpic.de.|Institute for Inorganic and Analytical Chemistry, Johannes Gutenberg University Mainz, Duesbergweg 10-14, 55128, Mainz, Germany. c.kampf@mpic.de.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Kampf
mesh_terms: D000336:Aerosols / Q000032:analysis*; D000393:Air Pollutants / Q000032:analysis*; D000485:Allergens / Q000032:analysis; D001272:Atmosphere / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis; D052638:Particulate Matter / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000336:Aerosols; D000393:Air Pollutants; D000485:Allergens; D001426:Bacterial Proteins; D005656:Fungal Proteins; D052638:Particulate Matter; D010940:Plant Proteins; D011506:Proteins
keywords: Atmospheric aerosols; Bioanalytical methods; HPLC; Mass spectrometry; Metaproteomics