title: Protein-protein interaction between ezrin and p65 in human breast cancer cells.
abstract: Our study aimed to investigate the co-localization and protein-protein interactions between ezrin and p65 in human breast cancer cells. Liquid chromatography-mass spectrometry (LCMS) was used to uncover novel protein interactions with ezrin in MDA-MB-231 cells. Endogenous co-immunoprecipitation was used to validate protein-protein interactions between ezrin and p65 in MDA-MB-231. Exogenous interactions between ezrin and p65 were validated in MDA-MB-231 cells via Flag-ezrin and HA-p65 co-transfection and followed by co-immunoprecipitation. Immunofluorescence staining was used to visualize ezrin and p65 co-localization in MDA-MB-231. LCMS results showed that there were 1000 proteins interacting with ezrin in MDA-MB-231 cells. Ezrin and p65 interactions were confirmed with both endogenous and exogenous methods. We were also able to visualize ezrin and p65 co-localization in MDA-MB-231. In summary, we found protein-protein interactions between Ezrin and p65 in human breast cancer cells.
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Baiyun District, Guangzhou, Guangdong Province, China.
authors.forename: R
authors.initials: R
authors.lastname: Tang
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Baiyun District, Guangzhou, Guangdong Province, China.
authors.forename: F X
authors.initials: FX
authors.lastname: Li
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Baiyun District, Guangzhou, Guangdong Province, China.
authors.forename: W F
authors.initials: WF
authors.lastname: Shao
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Baiyun District, Guangzhou, Guangdong Province, China.
authors.forename: Q S
authors.initials: QS
authors.lastname: Wen
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Baiyun District, Guangzhou, Guangdong Province, China.
authors.forename: X R
authors.initials: XR
authors.lastname: Yu
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Baiyun District, Guangzhou, Guangdong Province, China.
authors.forename: J B
authors.initials: JB
authors.lastname: Xiong
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D051996:Transcription Factor RelA / Q000378:metabolism*; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D051996:Transcription Factor RelA; D000099149:Ezrin