title: iTRAQ-Based Proteomics Investigation of Aqueous Humor from Patients with Coats' Disease.
abstract: BACKGROUND
  Coats' disease is an uncommon form of retinal telangiectasis, and the identification of novel proteins that contribute to the development of Coats' disease is useful for improving treatment efficacy. Proteomic techniques have been used to study many eye diseases; however, few studies have used proteomics to study the development of Coats' disease.
  
  
  METHODS
  Isobaric tagging for relative and absolute protein quantification (iTRAQ) was employed to screen differentially expressed proteins (DEPs) in the aqueous humor (AH) between stage 3A patients (n = 8), stage 3B patients (n = 14), stage 4 patients (n = 2) and control patients (n = 20). Differentially co-expressed proteins (DCPs) were present in all three stages of Coats' disease and were considered disease-specific proteins. These proteins were further analyzed using Gene Ontology (GO) functional annotations.
  
  
  RESULTS
  A total of 819 proteins were identified in the AH, 222 of which were significantly differentially expressed (fold change > 2 and P < 0.05) in the samples from at least one stage of Coats' disease. Of the DEPs, 46 were found among all three stages of Coats' disease and the controls; therefore, they were considered Coats' disease-specific proteins (DCPs). A GO classification analysis indicated that the DCPs were closely related to structural molecule activity, cell adhesion molecule binding and receptor binding. Western blotting confirmed the expression levels of haptoglobin and apolipoprotein C-I were significantly up-regulated in Coats' disease.
  
  
  CONCLUSIONS
  The 46 Coats' disease-specific proteins may provide additional insights into the mechanism of Coats' disease and represent potential biomarkers for identifying individuals with Coats' disease.
authors.affiliation: Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Intraocular Tumor Diagnosis and Treatment, Beijing Ophthalmology and Visual Science Key Lab, Beijing, 100730, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Intraocular Tumor Diagnosis and Treatment, Beijing Ophthalmology and Visual Science Key Lab, Beijing, 100730, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Lu
authors.affiliation: Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Intraocular Tumor Diagnosis and Treatment, Beijing Ophthalmology and Visual Science Key Lab, Beijing, 100730, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Song
authors.affiliation: Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Intraocular Tumor Diagnosis and Treatment, Beijing Ophthalmology and Visual Science Key Lab, Beijing, 100730, China.
authors.forename: Songfeng
authors.initials: S
authors.lastname: Li
authors.affiliation: Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Intraocular Tumor Diagnosis and Treatment, Beijing Ophthalmology and Visual Science Key Lab, Beijing, 100730, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Wei
mesh_terms: D000293:Adolescent; D000368:Aged; D053302:Apolipoprotein C-I / Q000378:metabolism*; D001082:Aqueous Humor / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D018450:Disease Progression; D006242:Haptoglobins / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D040901:Proteomics; D058456:Retinal Telangiectasis / Q000378:metabolism*; D012171:Retinal Vessels / Q000378:metabolism
chemical_list: D053302:Apolipoprotein C-I; D006242:Haptoglobins