title: Kiwifruit Allergy in Children: Characterization of Main Allergens and Patterns of Recognition.
abstract: Kiwifruit allergy has been described mostly in the adult population, but immunoglobulin (Ig)E-mediated allergic reactions to kiwifruit appear to be occurring more frequently in children. To date, 13 allergens from kiwifruit have been identified. Our aim was to identify kiwifruit allergens in a kiwifruit allergic-pediatric population, describing clinical manifestations and patterns of recognition. Twenty-four children were included. Diagnosis of kiwifruit allergy was based on compatible clinical manifestations and demonstration of specific IgE by skin prick test (SPT) and/or serum-specific IgE determination. SDS-PAGE and immunoblotting were performed with kiwifruit extract, and proteins of interest were further analyzed by mass spectrometry/mass spectrometry. For component-resolved in vitro diagnosis, sera of kiwifruit-allergic patients were analyzed by an allergen microarray assay. Act d 1 and Act d 2 were bound by IgE from 15 of 24 children. Two children with systemic manifestations recognized a protein of 15 kDa, homologous to Act d 5. Act d 1 was the allergen with the highest frequency of recognition on microarray chip, followed by Act d 2 and Act d 8. Kiwifruit allergic children develop systemic reactions most frequently following ingestion compared to adults. Act d 1 and Act d 2 are major allergens in the pediatric age group.
authors.affiliation: Department of Pediatrics, Children's Hospital Teresa Herrera, A Coru&#241;a University Hospital, Xubias de Arriba 84, 15006 La Coru&#241;a, Spain. ana.moreno.alvarez@sergas.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Moreno Álvarez
authors.affiliation: Department of Pediatrics, Children's Hospital Teresa Herrera, A Coru&#241;a University Hospital, Xubias de Arriba 84, 15006 La Coru&#241;a, Spain. leticia.vila.sexto@sergas.es.
authors.forename: Leticia Vila
authors.initials: LV
authors.lastname: Sexto
authors.affiliation: Division of Pediatric Allergy and Immunology and the Jaffe Food Allergy Institute, The Mount Sinai School of Medicine, New York, 10029-6574, NY, USA. luda.bardina@mssm.edu.
authors.forename: Luda
authors.initials: L
authors.lastname: Bardina
authors.affiliation: Division of Pediatric Allergy and Immunology and the Jaffe Food Allergy Institute, The Mount Sinai School of Medicine, New York, 10029-6574, NY, USA. galina.grishina@mssm.edu.
authors.forename: Galina
authors.initials: G
authors.lastname: Grishina
authors.affiliation: Division of Pediatric Allergy and Immunology and the Jaffe Food Allergy Institute, The Mount Sinai School of Medicine, New York, 10029-6574, NY, USA. hugh.sampson@mssm.edu.
authors.forename: Hugh A
authors.initials: HA
authors.lastname: Sampson
keywords: SDS-PAGE immunoblotting; actinidin; food allergy; kiwifruit; pediatric