title: Mitosis-associated repression in development.
abstract: Transcriptional repression is a pervasive feature of animal development. Here, we employ live-imaging methods to visualize the Snail repressor, which establishes the boundary between the presumptive mesoderm and neurogenic ectoderm of early Drosophila embryos. Snail target enhancers were attached to an MS2 reporter gene, permitting detection of nascent transcripts in living embryos. The transgenes exhibit initially broad patterns of transcription but are refined by repression in the mesoderm following mitosis. These observations reveal a correlation between mitotic silencing and Snail repression. We propose that mitosis and other inherent discontinuities in transcription boost the activities of sequence-specific repressors, such as Snail.
authors.affiliation: Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA; Department of Molecular and Cell Biology, Division of Genetics, Genomics, and Development, University of California at Berkeley, Berkeley, California 94720, USA;
authors.forename: Emilia
authors.initials: E
authors.lastname: Esposito
authors.affiliation: Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA;
authors.forename: Bomyi
authors.initials: B
authors.lastname: Lim
authors.affiliation: Department of Physics, Princeton University, Princeton, New Jersey 08544, USA;
authors.forename: Ghita
authors.initials: G
authors.lastname: Guessous
authors.affiliation: Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA;
authors.forename: Hanieh
authors.initials: H
authors.lastname: Falahati
authors.affiliation: Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA; Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Levine
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics; D004625:Embryo, Nonmammalian; D004742:Enhancer Elements, Genetic / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D008938:Mitosis / Q000235:genetics / Q000502:physiology*; D000071250:Snail Family Transcription Factors / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; D000071250:Snail Family Transcription Factors; C000604451:sna protein, Drosophila; C091428:sog protein, Drosophila
keywords: Drosophila embryo; live imaging; mitosis; repression; transcription