title: In Vitro and In Vivo Antiangiogenic Activity of Crowberry (Empetrum nigrum var. japonicum).
abstract: Crowberry, Empetrum nigrum var. japonicum, is widely used in folk medicine and grows naturally in Korea. Although some constituents and biological activity of Korean crowberry have been examined, there is little detailed information available. In this study, we investigated the effects of ethanol extracts of crowberry (EECB) on the inhibition of angiogenesis, both in vitro and in vivo. The effects of EECB were tested on in vitro models of angiogenesis, that is, tube formation and proliferation of human umbilical vein endothelial cells (HUVECs). EECB exhibited significant inhibitory effects on tube formation of HUVECs in a concentration-dependent manner. In addition, crowberry significantly suppressed the proliferation of HUVECs in a concentration-dependent manner. Furthermore, strong antiangiogenic activity of EECB samples was observed in the in vivo assay using chick embryo chorioallantoic membrane (CAM). These results indicate that crowberry may have potential applications in the prevention and treatment of angiogenesis-dependent human diseases.
authors.forename: Hong-Sook
authors.initials: HS
authors.lastname: Bae
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Kim
authors.forename: Da Hye
authors.initials: da H
authors.lastname: Jeong
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hosoya
authors.forename: Shigenori
authors.initials: S
authors.lastname: Kumazawa
authors.forename: Mira
authors.initials: M
authors.lastname: Jun
authors.forename: Oh-Yoen
authors.initials: OY
authors.lastname: Kim
authors.forename: Sun Wook
authors.initials: SW
authors.lastname: Kim
authors.forename: Mok-Ryeon
authors.initials: MR
authors.lastname: Ahn
mesh_terms: D020533:Angiogenesis Inhibitors / Q000032:analysis*; D000818:Animals; D002642:Chick Embryo; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry*
chemical_list: D020533:Angiogenesis Inhibitors