title: [Establishment of Quality Control System of Nucleic Acid Detection for Ebola Virus in Sierra Leone-China Friendship Biological Safety Laboratory].
abstract: The quality control process throughout the Ebola virus nucleic acid detection in Sierra Leone-China Friendship Biological Safety Laboratory (SLE-CHN Biosafety Lab) was described in detail, in order to comprehensively display the scientific, rigorous, accurate and efficient practice in detection of Ebola virus of first batch detection team in SLE-CHN Biosafety Lab. Firstly, the key points of laboratory quality control system was described, including the managements and organizing, quality control documents and information management, instrument, reagents and supplies, assessment, facilities design and space allocation, laboratory maintenance and biosecurity. Secondly, the application of quality control methods in the whole process of the Ebola virus detection, including before the test, during the test and after the test, was analyzed. The excellent and professional laboratory staffs, the implementation of humanized management are the cornerstone of the success; High-level biological safety protection is the premise for effective quality control and completion of Ebola virus detection tasks. And professional logistics is prerequisite for launching the laboratory diagnosis of Ebola virus. The establishment and running of SLE-CHN Biosafety Lab has landmark significance for the friendship between Sierra Leone and China, and the lab becomes the most important base for Ebola virus laboratory testing in Sierra Leone.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Kai
authors.initials: K
authors.lastname: Nie
authors.forename: Huanyu
authors.initials: H
authors.lastname: Wang
authors.forename: Haijun
authors.initials: H
authors.lastname: Du
authors.forename: Jingdong
authors.initials: J
authors.lastname: Song
authors.forename: Kang
authors.initials: K
authors.lastname: Xiao
authors.forename: Wenwen
authors.initials: W
authors.lastname: Lei
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Guo
authors.forename: Hejiang
authors.initials: H
authors.lastname: Wei
authors.forename: Kun
authors.initials: K
authors.lastname: Cai
authors.forename: Yanhai
authors.initials: Y
authors.lastname: Wang
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.forename: Bangura
authors.initials: B
authors.lastname: Gerald
authors.forename: Idrissa Laybohr
authors.initials: IL
authors.lastname: Kamara
authors.forename: Mifang
authors.initials: M
authors.lastname: Liang
authors.forename: Guizhen
authors.initials: G
authors.lastname: Wu
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Dong
mesh_terms: D002681:China; D029043:Ebolavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D007753:Laboratories / Q000592:standards*; D007757:Laboratory Infection; D011786:Quality Control; D012367:RNA, Viral / Q000235:genetics; D012807:Sierra Leone; D000078329:Workforce
chemical_list: D012367:RNA, Viral