title: Biochemical Analysis of Autophagy in Algae and Plants by Monitoring the Electrophoretic Mobility of ATG8.
abstract: Identification of specific autophagy markers has been fundamental to investigate autophagy as catabolic process. Among them, the ATG8 protein turned out to be one of the most widely used and specific molecular markers of autophagy both in higher and lower eukaryotes. Here, we describe how ATG8 can be used to monitor autophagy in Chlamydomonas and Arabidopsis by western blot analysis.
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Sevilla, Avda. Américo Vespucio, 49, 41092, Sevilla, Spain.
authors.forename: María Esther
authors.initials: ME
authors.lastname: Pérez-Pérez
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Sevilla, Avda. Américo Vespucio, 49, 41092, Sevilla, Spain.
authors.forename: Ascensión
authors.initials: A
authors.lastname: Andrés-Garrido
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Sevilla, Avda. Américo Vespucio, 49, 41092, Sevilla, Spain. crespo@ibvf.csic.es.
authors.forename: José L
authors.initials: JL
authors.lastname: Crespo
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000302:isolation & purification; D001343:Autophagy / Q000235:genetics*; D000071190:Autophagy-Related Protein 8 Family / Q000235:genetics* / Q000302:isolation & purification; D002696:Chlamydomonas / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*
chemical_list: C000597073:ATG8 protein, Arabidopsis; D029681:Arabidopsis Proteins; D000071190:Autophagy-Related Protein 8 Family
keywords: ATG8; Alga; Autophagy; Chlamydomonas; SDS-PAGE; Western blot