title: Evaluation and clinical significance of cyclin-dependent kinase5 expression in cervical lesions: a clinical research study in Guangxi, China.
abstract: BACKGROUND
  Studies have been reported that cyclin-dependent kinase5 (CDK5) was associated with the development of several cancers. However, the relationship between CDK5 level and clinicopathological factors is still poorly understood in cervical diseases. The aim of the current study was to investigate the expression of CDK5 and its clinical significance in variant cervical lesions.
  
  
  METHODS
  Immunohistochemistry (IHC) was used to detect CDK5 expression in 54 cases of chronic cervicitis, 42 cases of condyloma acuminate (CA), 38 cases of carcinoma in situ, and 360 cases of cervical cancers [adenocarcinoma, n = 63; squamous cell carcinoma (SCC), n = 263; adenosquamous carcinoma, n = 34]. The clinicopathological characteristics in relation to CDK5 were examined by Pearson's Chi-square test.
  
  
  RESULTS
  The positive rates of CDK5 were 27.8, 31.0, 50, 54.0, 58.8, and 62.7 % in chronic cervicitis, CA, carcinoma in situ, adenocarcinoma, adenosquamous carcinoma and SCC, respectively. Statistically analysis showed that CDK5 expression in cervical cancer tissues was higher than non-cervical cancer tissues (inflammation and CA) (P < 0.001). The overexpression of CDK5 was significantly correlated with lymph node metastasis (r = 0.317; P < 0.001), histological type (r = 0.198; P < 0.001), FIGO stage (r = 0.358; P < 0.001), TNM stage (r = 0.329; P < 0.001) and pathological grade (r = 0.259; P < 0.001) in cervical lesions evaluated by Pearson's Chi-square test. Furthermore, the positive relationships were found between CDK5 and lymph node metastasis (P < 0.001), FIGO stage (P < 0.001), TNM stage (P < 0.001) and pathological grade (P < 0.001) in SCC. CDK5 was positively interrelated to TNM stage (P = 0.017) in adenosquamous carcinoma.
  
  
  CONCLUSIONS
  CDK5 may play a vital role in the development of cervical cancer, which may be a marker for the diagnosis, therapy and prognosis of cervical cancer.
authors.affiliation: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Deng-Hua
authors.initials: DH
authors.lastname: Pan
authors.affiliation: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Zhu
authors.affiliation: Department of Children Rehabilitation Medicine, Guangxi Maternal and Child Health Hospital, 225 Xinyang Road, Nanning, 530003, Guangxi Zhuang Autonomous Region, China.
authors.forename: Xiao-Miao
authors.initials: XM
authors.lastname: Lin
authors.affiliation: Center for Genomic and Personalized Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Xing-Gu
authors.initials: XG
authors.lastname: Lin
authors.affiliation: Center for Genomic and Personalized Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Rong-Quan
authors.initials: RQ
authors.lastname: He
authors.affiliation: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Yan-Xin
authors.initials: YX
authors.lastname: Ling
authors.affiliation: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Shi-Tao
authors.initials: ST
authors.lastname: Su
authors.affiliation: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Madushi Mihiranganee
authors.initials: MM
authors.lastname: Wickramaarachchi
authors.affiliation: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Yi-Wu
authors.initials: YW
authors.lastname: Dang
authors.affiliation: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China. yxwwkl@163.com.
authors.forename: Kang-Lai
authors.initials: KL
authors.lastname: Wei
authors.affiliation: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China. chen_gang_triones@163.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology; D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000473:pathology; D002681:China; D051360:Cyclin-Dependent Kinase 5 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000473:pathology
chemical_list: D051360:Cyclin-Dependent Kinase 5; C495905:CDK5 protein, human
keywords: Cervical cancer; Chronic cervicitis; Condyloma acuminate; Cyclin-dependent kinase5; IHC