title: Graphene Oxide Based Fluorometric Detection of Hydrogen Peroxide in Milk.
abstract: We report a highly rapid, visual, precise, selective and sensitive analytical method for the determination of hydrogen peroxide (H₂O₂) in milk using Graphene oxide (GO) with 2',7'-dichlorfluorescein diacetate (DCFH-DA). A 1000 µL aliquots of 10-fold diluted samples (high and low-fat milk) directly onto the 100 µL of GO and 100 µL of 100 µM DCFH-DA produced green colour under Ultraviolet light at 365 nm. The analytical feature of our proposed method includes low detection limit (10 mmol mL⁻¹) and satisfactory recovery values for samples. The presence of H202 in milk is a major concern because it constitutes a public health hazard. Many milk indursties are using H₂O₂ as a preservative, but if the concentration increases then it causes so many health problems such as neurodegenerative disorders, cancer and diabetes. Present methods show an easy way for detecting H₂O₂ generally require considerable time and laboratory facilities. The chemical tests have sufficient sensitivity to detect wide linear range of H₂O₂ concentration.
authors.forename: Sitansu Sekhar
authors.initials: SS
authors.lastname: Nanda
authors.forename: Dong Kee
authors.initials: DK
authors.lastname: Yi
authors.forename: Kwangmeyung
authors.initials: K
authors.lastname: Kim
mesh_terms: D000818:Animals; D002417:Cattle; D005470:Fluorometry / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D006108:Graphite; D006861:Hydrogen Peroxide