title: Production of New Restriction Endonuclease VpaK111BI Recognizing Sequence 5'-GGWCC-3' in a Haemolysin-less Mutant of Vibrio parahaemolyticus.
abstract: High restriction endonuclease activity was found in a haemolysin-less mutant of the Vibrio parahaemolyticus 1743-1 strain. The endonuclease, named VpaK11BI, recognized the palindromic pentanucleotide sequence of 5'-GGWCC-3' and cleaved double-stranded DNA after the first G, which is exactly the same as the specificity of AvaII. The haemolysin-less mutant of V. parahaemolyticus is now available for producing the valuable restriction endonuclease on a commercial scale.
authors.affiliation: a Biotechnology Research Laboratories , Takara Shuzo Co., Ltd. , Seta 3-4-1, Otsu, Shiga 520-21 , Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ueno
authors.affiliation: a Biotechnology Research Laboratories , Takara Shuzo Co., Ltd. , Seta 3-4-1, Otsu, Shiga 520-21 , Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kita
authors.affiliation: a Biotechnology Research Laboratories , Takara Shuzo Co., Ltd. , Seta 3-4-1, Otsu, Shiga 520-21 , Japan.|b National Institute of Health Sciences , Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158 , Japan.
authors.forename: M
authors.initials: M
authors.lastname: Miyahara
authors.affiliation: a Biotechnology Research Laboratories , Takara Shuzo Co., Ltd. , Seta 3-4-1, Otsu, Shiga 520-21 , Japan.|b National Institute of Health Sciences , Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158 , Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ishiwata
authors.affiliation: a Biotechnology Research Laboratories , Takara Shuzo Co., Ltd. , Seta 3-4-1, Otsu, Shiga 520-21 , Japan.|b National Institute of Health Sciences , Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158 , Japan.
authors.forename: K
authors.initials: K
authors.lastname: Mise
authors.affiliation: a Biotechnology Research Laboratories , Takara Shuzo Co., Ltd. , Seta 3-4-1, Otsu, Shiga 520-21 , Japan.
authors.forename: I
authors.initials: I
authors.lastname: Kato
authors.affiliation: a Biotechnology Research Laboratories , Takara Shuzo Co., Ltd. , Seta 3-4-1, Otsu, Shiga 520-21 , Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ishino
keywords: AvaII isoschizomer; Vibrio parahaemolylicus; haemolysin-less mutant; restriction endonuclease