title: Clinical Regression and Remission of Primary Refractory Angiocentric Lymphoma Following Autologous Tumour Peptide Antigen-charged Dendritic Cells After High-dose Chemotherapy and Autologous Stem Cell Rescue.
abstract: We report on the case of a 34-year old man with a previously refractory high-grade non-Hodgkin's Lymphoma which regressed following dendritic cell based immunotherapy after high-dose chemotherapy. Antigen presenting cells known as dendritic cells were cultured from harvested autologous peripheral blood progenitor cells. The dendritic cells were then exposed to unfractionated tumour peptides derived from a skin biopsy section inflitrated with lymphoma. These charged dendritic cells infused into the patient together with autologous T-lymphocytes after high-dose chemotherapy with peripheral blood stem cell rescue. Although the lymphoma relapsed after the high-dose chemotherapy it regressed again following the dendritic cell infusion. The patient's T-lymphocytes demonstrated in vitro reactivity to tumour peptides whereas the lymphocytes of controls did not. We propose that the lymphoma regression occurred because of a T cell mediated response against the tumour induced by the charged dendritic cells.
authors.affiliation: a Department of Haematology , St. Bartholomew's and The Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, University of London , Whitechapel, London E1 2AD.
authors.forename: D
authors.initials: D
authors.lastname: Taussig
authors.affiliation: a Department of Haematology , St. Bartholomew's and The Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, University of London , Whitechapel, London E1 2AD.
authors.forename: M A
authors.initials: MA
authors.lastname: Williams
authors.affiliation: a Department of Haematology , St. Bartholomew's and The Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, University of London , Whitechapel, London E1 2AD.
authors.forename: C
authors.initials: C
authors.lastname: Wiggins
authors.affiliation: b Histopathology , St. Bartholomew's and The Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, University of London , Whitechapel, London E1 2AD.
authors.forename: R M
authors.initials: RM
authors.lastname: Feakins
authors.affiliation: a Department of Haematology , St. Bartholomew's and The Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, University of London , Whitechapel, London E1 2AD.
authors.forename: N
authors.initials: N
authors.lastname: McBride
authors.affiliation: a Department of Haematology , St. Bartholomew's and The Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, University of London , Whitechapel, London E1 2AD.
authors.forename: A C
authors.initials: AC
authors.lastname: Newland
authors.affiliation: a Department of Haematology , St. Bartholomew's and The Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, University of London , Whitechapel, London E1 2AD.
authors.forename: S M
authors.initials: SM
authors.lastname: Kelsey
keywords: DC-dendritic cells; GM-CSF-granulocyte-macrophage colony-stimulating factor; IL-2-interleukin-2; T-lymphocytes; angiocentric T-cell lymphoma; high-dose chemotherapy; immunotherapy