title: Mapping the protein-binding sites for iridium(iii)-based CO-releasing molecules.
abstract: A combination of mass spectrometry, Raman microspectroscopy, circular dichroism and X-ray crystallography has been used to obtain detailed information on the reaction of an iridium-based CO-releasing molecule (Ir-CORM), Cs2IrCl5CO, with a model protein, bovine pancreatic ribonuclease. The results show that Ir-compound fragments bind to the N-terminal amine and close to histidine and methionine side chains, and the CO ligand is retained for a long time. The data provide helpful information for identifying protein targets for Ir-CORMs and for studying the mechanism that allows them to exhibit their interesting biological properties.
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario di Monte Sant'Angelo, Via Cintia, I-80126, Napoli, Italy. antonello.merlino@unina.it.
authors.forename: Marco
authors.initials: M
authors.lastname: Caterino
authors.affiliation: Departamento de Química Inorgánica, Analítica y Química Física/INQUIMAE-CONICET, University of Buenos Aires, Ciudad Universitaria, Pab. 2, C1428EHA Buenos Aires, Argentina.
authors.forename: Ariel A
authors.initials: AA
authors.lastname: Petruk
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario di Monte Sant'Angelo, Via Cintia, I-80126, Napoli, Italy. antonello.merlino@unina.it and CNR Institute of Biostructures and Bioimages, Via Mezzocannone 16, Napoli, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Vergara
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario di Monte Sant'Angelo, Via Cintia, I-80126, Napoli, Italy. antonello.merlino@unina.it.
authors.forename: Giarita
authors.initials: G
authors.lastname: Ferraro
authors.affiliation: CNR Institute of Biostructures and Bioimages, Via Mezzocannone 16, Napoli, Italy and Department of Pharmacy, University of Naples Federico II, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Marasco
authors.affiliation: Departamento de Química Inorgánica, Analítica y Química Física/INQUIMAE-CONICET, University of Buenos Aires, Ciudad Universitaria, Pab. 2, C1428EHA Buenos Aires, Argentina.
authors.forename: Fabio
authors.initials: F
authors.lastname: Doctorovich
authors.affiliation: Departamento de Química Inorgánica, Analítica y Química Física/INQUIMAE-CONICET, University of Buenos Aires, Ciudad Universitaria, Pab. 2, C1428EHA Buenos Aires, Argentina.
authors.forename: Dario A
authors.initials: DA
authors.lastname: Estrin
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario di Monte Sant'Angelo, Via Cintia, I-80126, Napoli, Italy. antonello.merlino@unina.it and CNR Institute of Biostructures and Bioimages, Via Mezzocannone 16, Napoli, Italy.
authors.forename: Antonello
authors.initials: A
authors.lastname: Merlino
mesh_terms: D001665:Binding Sites; D002248:Carbon Monoxide / Q000737:chemistry*; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D007495:Iridium / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D011506:Proteins; D007495:Iridium; D002248:Carbon Monoxide