title: Breeding of a 5-Fluorouridine-resistant Mutant with Increased Cellulose Production from Acetobacter xylinum subsp. nonacetoxidans.
abstract: UDP-glucose (UDP-G), the direct precursor of cellulose, is known to be produced from UTP and glucose-1-phosphate. In an attempt to increase UTP biosynthesis, 5-fluorouridine (5-FUR: a pyrimidine analog)-resistant mutants were obtained using Acetobacter xylinum subsp. nonacetoxidans 757 as the parent strain. One of the 5-FUR-resistant mutants, FUR-35, showed about 40% higher cellulose productivion compared to the parent strain. Intracellular levels of UTP and UDP-G in FUR-35 was found to be higher than those in the parent strain. The carbamyl phosphate synthetase II (CPS) activity of FUR-35 was higher than that of the parent strain and the feedback inhibition of CPS by UTP in FUR-35 had been released compared with that in the parent strain. These results suggest that the increased cellulose production of FUR-35 was attributable to its higher of intracellular UDP-G level resulting from increased UTP biosynthesis.
authors.affiliation: a Bio-Polymer Research Co., Ltd.
authors.forename: A
authors.initials: A
authors.lastname: Ishikawa
authors.affiliation: a Bio-Polymer Research Co., Ltd.
authors.forename: N
authors.initials: N
authors.lastname: Tonouchi
authors.affiliation: a Bio-Polymer Research Co., Ltd.
authors.forename: T
authors.initials: T
authors.lastname: Tsuchida
authors.affiliation: a Bio-Polymer Research Co., Ltd.
authors.forename: F
authors.initials: F
authors.lastname: Yoshinaga
keywords: 5-fluorouridine; Acetobacter; UDP-glucose; carbamyl phosphate synthetase II; cellulose