title: In vitro cytokine profile revealed differences from dorsal and ventral skin susceptibility to pathogen-probiotic interaction in gilthead seabream.
abstract: Skin is the first barrier of defense on fish, which is crucial to protection against different stressors, including pathogens. Skin samples obtained from dorsal and ventral part of Sparus aurata specimens were incubated with Photobacterium damselae subsp. piscicida (a pathogen for this fish species), with Shewanella putrefaciens Pdp11 (a probiotic bacteria isolated from healthy gilthead seabream skin) or with both bacteria. The gene expression profile of nine cytokines (il1b, tnfa, il6, il7, il8, il15, il18, il10 and tgfb) was studied by qPCR in all the skin samples. The present findings revealed different patterns of cytokine profile in dorsal and ventral skin of gilthead seabream, which could be related to the influence and susceptibility to a possible infection.
authors.affiliation: Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
authors.forename: Héctor
authors.initials: H
authors.lastname: Cordero
authors.affiliation: Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain; Marine Immunobiology Laboratory, Department of Biological Chemical Pharmaceutical Science and Technology, University of Palermo, Via Archirafi 18, Palermo, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Mauro
authors.affiliation: Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Cuesta
authors.affiliation: Marine Immunobiology Laboratory, Department of Biological Chemical Pharmaceutical Science and Technology, University of Palermo, Via Archirafi 18, Palermo, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Cammarata
authors.affiliation: Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain. Electronic address: aesteban@um.es.
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Esteban
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D004198:Disease Susceptibility / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D016905:Gram-Negative Bacterial Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D010776:Photobacterium / Q000502:physiology; D019936:Probiotics; D021541:Sea Bream; D020614:Shewanella putrefaciens / Q000737:chemistry
chemical_list: D016207:Cytokines; D029941:Fish Proteins
keywords: Cytokine; Gilthead seabream (Sparus aurata); Pathogen; Probiotic; Skin