title: Heterologous Expression of Fungal Secondary Metabolite Pathways in the Aspergillus nidulans Host System.
abstract: Heterologous expression of fungal secondary metabolite genes allows for the product formation of otherwise silent secondary metabolite biosynthesis pathways. It also allows facile expression of mutants or combinations of genes not found in nature. This capability makes model fungi an ideal platform for synthetic biology. In this chapter a detailed description is provided of how to heterologously express any fungal secondary metabolite gene(s) in a well-developed host strain of Aspergillus nidulans. It covers all the necessary steps from identifying a gene(s) of interest to culturing mutant strains to produce secondary metabolites.
authors.affiliation: School of Pharmacy, University of Southern California, Los Angeles, CA, United States.
authors.forename: J W A
authors.initials: JW
authors.lastname: van Dijk
authors.affiliation: School of Pharmacy, University of Southern California, Los Angeles, CA, United States; Dornsife College of Letters, Arts, and Sciences, University of Southern California, Los Angeles, CA, United States. Electronic address: clayw@usc.edu.
authors.forename: C C C
authors.initials: CC
authors.lastname: Wang
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways; D004271:DNA, Fungal / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D018014:Gene Transfer Techniques; D005800:Genes, Fungal; D016133:Polymerase Chain Reaction / Q000379:methods; D058615:Synthetic Biology / Q000379:methods*; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal
keywords: Aspergillus nidulans; Biosynthesis; Fungi; Heterologous expression; Natural product; Secondary metabolite