title: Development of immune-affinity 96 spots monolith array for multiple mycotoxins detection in food samples.
abstract: In this paper, a novel highly sensitive chemiluminescence immune-affinity 96 spots monolith array was developed to detect deoxynivalenol (DON), zearalenone (ZEN), T-2 toxin (T-2), and fumonisin B1 (FB1) in corn samples. Firstly, the monolith array was prepared through on suit UV-initiated copolymerization using polyethylene glycol diacrylate (PEGDA) as cross-linker, glycidyl methacrylate (GMA) as functional monomer and polyethylene glycol 200 (PEG 200) as the porogen. Subsequently, the four mycotoxins immune-affinity monolith array was prepared by immobilization of DON, ZEN, T-2, and FB1 antibody. The mole ratio of PEGDA/GMA, UV exposure time, and the volume ratio of PEG 200/PEGDA were optimized to improve the performances of the immune-affinity monolith array. For the mycotoxins immune-affinity monolith array based on chemiluminescence detection, the limit of detection was 0.0036ng/mL (DON), 0.0048ng/mL (ZEN), 0.0039ng/mL (T-2), and 0.0017ng/mL (FB1), respectively. The linear response in the range of 0.01-0.1ng/mL (R(2)=0.98). The results showed that the proposed four mycotoxins immune-affinity monolith array was a stable, accurate, and highly sensitive method to determine levels of DON, ZEN, T-2, and FB1 in real samples.
authors.affiliation: Institute of Facilities and Equipment in Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. Electronic address: muzishuiqudou@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Facilities and Equipment in Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Li-Ru
authors.initials: LR
authors.lastname: Xia
authors.affiliation: Institute of Facilities and Equipment in Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Yong-Fu
authors.initials: YF
authors.lastname: Zhao
authors.affiliation: Institute of Facilities and Equipment in Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: He-Ye
authors.initials: HY
authors.lastname: Wang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D004867:Equipment Design; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D037341:Fumonisins / Q000032:analysis*; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D013605:T-2 Toxin / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry / Q000382:microbiology; D015025:Zearalenone / Q000032:analysis*
chemical_list: D055910:Antibodies, Immobilized; D037341:Fumonisins; D014255:Trichothecenes; C056933:fumonisin B1; D015025:Zearalenone; D013605:T-2 Toxin; C007262:deoxynivalenol
keywords: Chemiluminescent detection; Immune-affinity array; Monolith; Mycotoxins; UV-initiated copolymerization