title: Defects in dermal Vγ4 γ δ T cells result in delayed wound healing in diabetic mice.
abstract: The skin serves as a physical and chemical barrier to provide an initial line of defense against environmental threats; however, this function is impaired in diabetes. Vγ4 γ δ T cells in the dermis are an important part of the resident cutaneous immunosurveillance program, but these cells have yet to be explored in the context of diabetes. In this study, we observed that the impaired maintenance of dermal Vγ4 γ δ T cells is caused by reduced production of IL-7 in the skin of diabetic mice, which was closely associated with weakened activation of the mTOR pathway in the epidermis of diabetic mice. Weakened CCL20/CCR6 chemokine signaling resulted in the impaired recruitment of dermal Vγ4 γ δ T cells following wounding in diabetic mice. Meanwhile, reduced levels of IL-23 and IL-1β in the dermis around the wounds of diabetic mice resulted in the impaired production of IL-17 by dermal Vγ4 γ δ T cells. Therefore, diminished dermal Vγ4 γ δ T cells and impaired IL-17 production by these cells were important factors in the markedly reduced IL-17 levels in the skin around the wounds of diabetic mice. Because reduced IL-17 levels at the wound edge have been closely associated with delayed wound closure in diabetic mice, defects in dermal Vγ4 γ δ T cells may be an important mechanism underlying delayed wound healing in diabetic mice.
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080, Guangdong, China.
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080, Guangdong, China.
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, The Third Military Medical UniversityChongqing 400038, China; Chongqing Key Laboratory for Disease ProteomicsChongqing 400038, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, The Third Military Medical UniversityChongqing 400038, China; Chongqing Key Laboratory for Disease ProteomicsChongqing 400038, China.
authors.forename: Guangping
authors.initials: G
authors.lastname: Liang
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080, Guangdong, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080, Guangdong, China.
authors.forename: Julin
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080, Guangdong, China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080, Guangdong, China.
authors.forename: Jingling
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080, Guangdong, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shu
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080, Guangdong, China.
authors.forename: Shaohai
authors.initials: S
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, The Third Military Medical UniversityChongqing 400038, China; Chongqing Key Laboratory for Disease ProteomicsChongqing 400038, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, The Third Military Medical UniversityChongqing 400038, China; Chongqing Key Laboratory for Disease ProteomicsChongqing 400038, China.
authors.forename: Gaoxing
authors.initials: G
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, The Third Military Medical UniversityChongqing 400038, China; Chongqing Key Laboratory for Disease ProteomicsChongqing 400038, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, The Third Military Medical UniversityChongqing 400038, China; Chongqing Key Laboratory for Disease ProteomicsChongqing 400038, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080, Guangdong, China.
authors.forename: Xusheng
authors.initials: X
authors.lastname: Liu
keywords: Diabetes; dermal Vγ4 γ δ T cells; wound healing