title: Profiling of Host Cell Response to Successive Canine Parvovirus Infection Based on Kinetic Proteomic Change Identification.
abstract: Canine parvovirus (CPV) reproduces by co-opting the resources of host cells, inevitably causing cytotoxic effects to the host cells. Feline kidney F81 cells are sensitive to CPV infection and show disparate growing statuses at different time points post-infection. This study analysed the response of F81 cells to CPV infection at successive infection time points by iTRAQ-based quantitative proteomics. Differentially expressed proteins (DEPs) during 60 h of infection and at selected time points post-infection were identified by an analysis of variance test and a two-tailed unpaired t test, respectively. DEPs with similar quantitative changes were clustered by hierarchical clustering and analysed by gene ontology enrichment, revealing that 12 h and 60 h post-infection were the optimal times to analyse the autonomous parvovirus replication and apoptosis processes, respectively. Using the Metacore(TM) database, 29 DEPs were enriched in a network involved in p53 regulation. Besides, a significantly enriched pathway suggests that the CPV-induced cytopathic effect was probably due to the deficiency of functional CFTR caused by CPV infection. This study uncovered the systemic changes in key cellular factors involved in CPV infection and help to understand the molecular mechanisms of the anti-cancer activity of CPV and the cytopathic effects induced by CPV infection.
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Yuening
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Jianke
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lin
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yi
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Yaru
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Jingqiang
authors.initials: J
authors.lastname: Ren
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Tong
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Cao
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Deng
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Shipeng
authors.initials: S
authors.lastname: Cheng
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005254:Feline Panleukopenia / Q000378:metabolism* / Q000821:virology; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D054884:Host-Pathogen Interactions; D010322:Parvoviridae Infections / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000502:physiology*; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods*; D013995:Time; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014779:Virus Replication
chemical_list: D016159:Tumor Suppressor Protein p53; D019005:Cystic Fibrosis Transmembrane Conductance Regulator