title: Understanding global changes of the liver proteome during murine schistosomiasis using a label-free shotgun approach.
abstract: Schistosomiasis is an endemic disease affecting over 207 million people worldwide caused by helminth parasites of the genus Schistosoma. In Brazil the disease is responsible for the loss of up to 800 lives annually, resulting from the desabilitating effects of this chronic condition. In this study, we infected Balb/c mice with Schistosoma mansoni and analysed global changes in the proteomic profile of soluble liver proteins. Our shotgun analyses revealed predominance of up-regulation of proteins at 5weeks of infection, coinciding with the onset of egg laying, and a remarkable down-regulation of liver constituents at 7weeks, when severe tissue damage is installed. Representatives of glycolytic enzymes and stress response (in particular at the endoplasmic reticulum) were among the most differentially expressed molecules found in the infected liver. Collectively, our data contribute over 70 molecules not previously reported to be found at altered levels in murine schistosomiasis to further exploration of their potential as biomarkers of the disease. Moreover, understanding their intricate interaction using bioinformatics approach can potentially bring clarity to unknown mechanisms linked to the establishment of this condition in the vertebrate host.
  
  
  
  To our knowledge, this study refers to the first shotgun proteomic analysis to provide an inventory of the global changes in the liver soluble proteome caused by Schistosoma mansoni in the Balb/c model. It also innovates by yielding data on quantification of the identified molecules as a manner to clarify and give insights into the underlying mechanisms for establishment of Schistosomiasis, a neglected tropical disease with historical prevalence in Brazil.
authors.affiliation: Programa de Pós Graduação em Bioengenharia, Universidade Federal de São João del Rei, São João del Rei, MG, Brazil; Programa de Pós Graduação em Biotecnologia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
authors.forename: Jonatan Marques
authors.initials: JM
authors.lastname: Campos
authors.affiliation: Programa de Pós Graduação em Biotecnologia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
authors.forename: Leandro Xavier
authors.initials: LX
authors.lastname: Neves
authors.affiliation: Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.
authors.forename: Nívia Carolina Nogueira
authors.initials: NC
authors.lastname: de Paiva
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
authors.forename: Renata Alves
authors.initials: RA
authors.lastname: de Oliveira E Castro
authors.affiliation: Programa de Pós Graduação em Biotecnologia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
authors.forename: Ana Helena
authors.initials: AH
authors.lastname: Casé
authors.affiliation: Departamento de Análises Clínicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
authors.forename: Cláudia Martins
authors.initials: CM
authors.lastname: Carneiro
authors.affiliation: Departamento de Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
authors.forename: Milton Hércules Guerra
authors.initials: MH
authors.lastname: Andrade
authors.affiliation: Departamento de Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil. Electronic address: williamcborges@hotmail.com.
authors.forename: William
authors.initials: W
authors.lastname: Castro-Borges
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D059865:Endoplasmic Reticulum Stress; D005786:Gene Expression Regulation; D006019:Glycolysis; D054884:Host-Pathogen Interactions; D008018:Life Cycle Stages / Q000235:genetics; D008099:Liver / Q000737:chemistry* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D012552:Schistosomiasis / Q000469:parasitology*; D012555:Schistosomiasis mansoni; D013312:Stress, Physiological
chemical_list: D015415:Biomarkers; D020543:Proteome
keywords: Label-free; Liver biomarkers; Schistosoma mansoni; Schistosomiasis; Shotgun proteomics