title: Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii.
abstract: Small heat shock proteins (HSPs) are molecular chaperones with ATP-independent properties. They are involved in a variety of physiological and stress processes. In this study, the full-length HSP 20 (HSP20) from Pinctada martensii, designated as PmHSP20, was obtained from hemocytes using rapid amplification of cDNA ends technology. The PmHSP20 cDNA was 952 bp in length, containing an open reading frame of 534 bp that encoded 177-amino acid residues, with an isoelectric point of 5.86 and molecular weight of 20.24 kDa. The sequence of this deduced polypeptide contained typical structure and function domains conserved in the HSP20 family, providing evidence that PmHSP20 belongs to the HSP20 family. The PmHSP20 mRNA expression levels were detected in various tissues of P. martensii and in hemocytes after challenges with the bacteria Vibrio harveyi and lipopolysaccharide (LPS) using quantitative real-time polymerase chain reaction amplification. The results indicated that PmHSP20 is constitutively expressed in all tissues tested and might be involved in the immune response. The upregulation of PmHSP20 after V. harveyi and LPS challenge suggests that PmHSP20 plays an important role in anti-bacterial immunity. Studies on PmHSP20 are a valuable resource to further explore the immune system in pearl oysters and might enhance our knowledge of molluscan innate immunity.
authors.affiliation: Fisheries College, Guangdong Ocean University, Zhanjiang, China.
authors.forename: Q N
authors.initials: QN
authors.lastname: Lei
authors.affiliation: Fisheries College, Guangdong Ocean University, Zhanjiang, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Wu
authors.affiliation: Fisheries College, Guangdong Ocean University, Zhanjiang, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Liang
authors.affiliation: Fisheries College, Guangdong Ocean University, Zhanjiang, China.
authors.forename: Z X
authors.initials: ZX
authors.lastname: Wang
authors.affiliation: Fisheries College, Guangdong Ocean University, Zhanjiang, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Fisheries College, Guangdong Ocean University, Zhanjiang, China.
authors.forename: Y W
authors.initials: YW
authors.lastname: Deng
mesh_terms: D000818:Animals; D050886:HSP20 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006434:Hemocytes / Q000378:metabolism / Q000382:microbiology; D049911:Pinctada / Q000235:genetics* / Q000378:metabolism; D000072417:Protein Domains; D013312:Stress, Physiological; D015854:Up-Regulation; D014733:Vibrio / Q000472:pathogenicity
chemical_list: D050886:HSP20 Heat-Shock Proteins