title: Effects of Bio-Oss®and Cerasorb®dental M on the expression of bone-remodeling mediators in human monocytes.
abstract: In contribution to diverse techniques of bone reconstruction involving biomaterials in contemporary dentistry, this study aimed to evaluate the effect of the bone-grafting materials Bio-Oss®and Cerasorb®Dental M on the expression of cytokines associated with bone remodeling by human monocytes in vitro. Bio-Oss®and Cerasorb®Dental M were incubated in separate culture media, and their supernatants were added to mononuclear cells of human peripheral blood, some of which had been stimulated with Porphyromonas gingivalis. The frequency of total monocytes and CD14+monocytes producing cytokines interleukin 6 (IL-6), IL-8, IL-10, IL-12, and tumor necrosis factor alpha (TNF-α) were determined by flow cytometry. One-way analysis of variance with repeated measures, followed by Tukey's post hoc test, revealed that stimulation with P. gingivalis increased the expression of IL-6 and IL-8 and reduced the expression of TNF-α compared to effects demonstrated in the control group (p < 0.05). Adding biomaterial supernatants did not significantly affect the expression of any cytokine evaluated, however, either in the absence or in the presence of bacterial stimulation. Our data suggest that Bio-Oss®and Cerasorb®Dental M neither stimulate cytokine production in human monocytes nor interfere with mechanisms of cell communication mediated by cytokines evaluated during stimulation with P. gingivalis. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2066-2073, 2017.
authors.affiliation: Graduate Program in Dentistry, Department of Dentistry, Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Filipe
authors.initials: F
authors.lastname: de Assis Gonzaga
authors.affiliation: Graduate Program in Dentistry, Department of Dentistry, Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Tatiana Teixeira
authors.initials: TT
authors.lastname: de Miranda
authors.affiliation: Department of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Luisa Mourão Dias
authors.initials: LMD
authors.lastname: Magalhães
authors.affiliation: Department of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.|Instituto Nacional de Ciência e Tecnologia, Doenças Tropicais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Walderez Ornelas
authors.initials: WO
authors.lastname: Dutra
authors.affiliation: Instituto Nacional de Ciência e Tecnologia, Doenças Tropicais, Belo Horizonte, Minas Gerais, Brazil.|Núcleo de Ensino e Pesquisa do Instituto Mário Penna, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Kenneth John
authors.initials: KJ
authors.lastname: Gollob
authors.affiliation: Graduate Program in Dentistry, Department of Dentistry, Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Paulo Eduardo Alencar
authors.initials: PEA
authors.lastname: Souza
authors.affiliation: Graduate Program in Dentistry, Department of Dentistry, Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Martinho Campolina Rebello
authors.identifier: 0000-0003-0192-5614
authors.initials: MCR
authors.lastname: Horta
mesh_terms: D016723:Bone Remodeling; D002130:Calcium Phosphates / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008422:Materials Testing; D008903:Minerals / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D016966:Porphyromonas gingivalis / Q000737:chemistry
chemical_list: C077540:Bio-Oss; D002130:Calcium Phosphates; D016207:Cytokines; D008903:Minerals; C485817:beta-tricalcium phosphate
keywords: biomaterials; cytokines; dentistry; implantology; monocytes