title: A Multichannel Bioluminescence Determination Platform for Bioassays.
abstract: The present protocol introduces a multichannel bioluminescence determination platform allowing a high sample throughput determination of weak bioluminescence with reduced standard deviations. The platform is designed to carry a multichannel conveyer, an optical filter, and a mirror cap. The platform enables us to near-simultaneously determine ligands in multiple samples without the replacement of the sample tubes. Furthermore, the optical filters beneath the multichannel conveyer are designed to easily discriminate colors during assays. This optical system provides excellent time- and labor-efficiency to users during bioassays.
authors.affiliation: Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), Onogawa 16-1, Tsukuba, Ibaraki, 305-8569, Japan. kimu-sb@aist.go.jp.
authors.forename: Sung-Bae
authors.initials: SB
authors.lastname: Kim
authors.affiliation: Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), Onogawa 16-1, Tsukuba, Ibaraki, 305-8569, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Naganawa
mesh_terms: D000818:Animals; D001681:Biological Assay; D019556:COS Cells; D002522:Chlorocebus aethiops; D015870:Gene Expression; D017930:Genes, Reporter; D006728:Hormones / Q000378:metabolism; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods; D015335:Molecular Probes; D011993:Recombinant Fusion Proteins; D012463:Saliva / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D006728:Hormones; D015335:Molecular Probes; D011993:Recombinant Fusion Proteins
keywords: Assay device; Bioluminescence; Cortisol; Single-chain probe; Steroid hormone