title: A C-type lectin with an immunoglobulin-like domain promotes phagocytosis of hemocytes in crayfish Procambarus clarkii.
abstract: C-type lectins are important immune molecules that participate in host defense response. The present work reports a novel C-type lectin (PcLec3) from the red swamp crayfish Procambarus clarkii. Sequence analysis found that PcLec3 encodes a polypeptide with252 amino acid residues, which contains an immunoglobulin-like domain (IG) and a C-type lectin domain (CTLD) arranged in tandem. Tissue distribution analysis indicated that PcLec3 is enriched expressed in hemocytes and hepatopancreas cells, in which PcLec3 was up-regulated following bacterial challenge by Vibrio anguillarum. Function analysis using recombinant full-length PcLec3, IG, and CTLD proteins revealed that these recombinant proteins had the capacity to bind carbohydrates and bacteria, while IG determined the cell binding activity. However, only full-length PcLec3 promotes the phagocytic activity of hemocytes and subsequent clearance of invasive bacteria. Taken together, these results manifest that PcLec3 acts as a hemocyte adhesion molecule to promote hemocyte phagocytosis against invasive V. anguillarum.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, China.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Zhang
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of life science, Shandong University, Jinan, Shandong, 250100, China.
authors.forename: Xian-Wei
authors.initials: XW
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of life science, Shandong University, Jinan, Shandong, 250100, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of life science, Shandong University, Jinan, Shandong, 250100, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of life science, Shandong University, Jinan, Shandong, 250100, China.
authors.forename: Jin-Xing
authors.initials: JX
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea / Q000166:cytology* / Q000821:virology; D001483:Base Sequence; D002448:Cell Adhesion / Q000187:drug effects; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D006434:Hemocytes / Q000166:cytology* / Q000187:drug effects; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D000070557:Immunoglobulin Domains; D037181:Lectins, C-Type / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000073893:Sugars / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D014733:Vibrio / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D037181:Lectins, C-Type; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000073893:Sugars