title: [Generation of Japanese Encephalitis Virus-like Particle Vaccine and Preliminary Evaluation of Its Protective Efficiency].
abstract: The cDNA fragment of JEV prME gene was cloned into the baculovirus shuttle vector (bacmid) to construct a recombinant baculovirus vector, defined as AcBac-prME. Then the recombinant baculovirus Ac-prME was obtained by transfecting Sf9 cells with AcBac-prME. Western blot analysis and immunofluorescence results indicated that both prM and E proteins were efficiently expressed in Sf9 cells. Electron microscopy suggested that prME was assembled into JEV-VLPs. To further evaluate the potential of JEV-VLPs as vaccine, the mice were immunized with JEV-VLPs and then challenged with lethal JEV. The results of mice survival and pathological changes demonstrated that the JEV-VLPs performed complete protection against JEV-P3 strain and relieved pathological changes in the mice brain significant. This study suggest that JEV-VLPs would be a potential vaccine for Japanese encephalitis virus.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ruikun
authors.initials: R
authors.lastname: Du
authors.forename: Shaomei
authors.initials: S
authors.lastname: Huang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Jinliang
authors.initials: J
authors.lastname: Liu
authors.forename: Bibo
authors.initials: B
authors.lastname: Zhu
authors.forename: Hualin
authors.initials: H
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Deng
authors.forename: Shengbo
authors.initials: S
authors.lastname: Cao
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D006801:Humans; D022321:Japanese Encephalitis Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D061987:Sf9 Cells; D014611:Vaccination; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D022321:Japanese Encephalitis Vaccines; D058425:Vaccines, Virus-Like Particle; D014759:Viral Envelope Proteins