title: Hyperadherence of Pseudomonas taiwanensis VLB120ΔC increases productivity of (S)-styrene oxide formation.
abstract: The attachment strength of biofilm microbes is responsible for the adherence of the cells to surfaces and thus is a critical parameter in biofilm processes. In tubular microreactors, aqueous-air segmented flow ensures an optimal oxygen supply and prevents excessive biofilm growth. However, organisms growing in these systems depend on an adaptation phase of several days, before mature and strong biofilms can develop. This is due to strong interfacial forces. In this study, a hyperadherent mutant of Pseudomonas taiwanensis VLB120ΔCeGFP possessing an engineered cyclic diguanylate metabolism, was applied to a continuous biofilm process for the production of (S)-styrene oxide. Cells of the mutant P.  taiwanensis VLB120ΔCeGFP Δ04710, showing the same specific activity as the wild type, adhered substantially stronger to the substratum. Adaptation to the high interfacial forces was not necessary in these cases. Thereby, 40% higher final product concentrations were achieved and the maximal volumetric productivity of the parent strain was significantly surpassed by P. taiwanensis VLB120ΔCeGFP Δ04710. Applying mutants with strong adhesion in biofilm-based catalysis opens the door to biological process control in future applications of catalytic biofilms using other industrially relevant strains.
authors.affiliation: Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Strasse 66, 44227, Dortmund, Germany.|Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany.
authors.forename: Karolin
authors.initials: K
authors.lastname: Schmutzler
authors.affiliation: Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Strasse 66, 44227, Dortmund, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Kupitz
authors.affiliation: Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schmid
authors.affiliation: Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Buehler
mesh_terms: D001422:Bacterial Adhesion; D019149:Bioreactors / Q000382:microbiology; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism; D004852:Epoxy Compounds / Q000378:metabolism*; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics; D011549:Pseudomonas / Q000378:metabolism / Q000502:physiology*
chemical_list: D004852:Epoxy Compounds; C062025:bis(3',5')-cyclic diguanylic acid; C013690:styrene oxide; D006152:Cyclic GMP