title: Evaluation of the Antioxidant and Antiproliferative Effects of Three Peptide Fractions of Germinated Soybeans on Breast and Cervical Cancer Cell Lines.
abstract: Soybeans are an important source of bioactive molecules, such as peptides, which generation can improve through germination. In this study, the antioxidant and antiproliferative activities of three peptide fractions (>10 kDa, 5-10 kDa and <5 kDa) that were obtained by ultrafiltration of soybean protein hydrolysate after six days of germination were evaluated. The antioxidant activities of the peptide fractions were assessed by reducing power, Cu+2and Fe+2chelation and OH· scavenging assays, whereas their antiproliferative effects against cervical (HeLa, SiHa, CasKi) and breast (MCF7 and MDA-MB-231) cancer cell lines were evaluated by the MTT assay. Apoptosis was determined by Hoechst-PI staining. The most active peptide fraction (MAPF) was the >10 kDa fraction, which showed the greatest antioxidant and antiproliferative activity. The most sensitive cancer cell lines were the HeLa, CasKi and MDA-MB-231 cells, which had IC50values of 16.2, 14.3 and 15.2 mg/mL, respectively, and apoptotic indices above 50 % after 6 or 8 h of exposure. The effect of MAPF on normal cells (HaCaT) was minimal. The amino acid composition of MAPF was characterized by high proline, phenylalanine and tyrosine content, and MALDI-TOF/TOF analysis showed six signals with molecular weights of 12 to 42 kDa.
authors.affiliation: Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Campus Zacatenco, Unidad Profesional "Adolfo López Mateos", Calle Wilfrido Massieu esquina Cda. Manuel Stampa. C.P, 07738, Ciudad de México, México.
authors.forename: González-Montoya
authors.initials: GM
authors.lastname: Marcela
authors.affiliation: Lab. de Citopatologia Ambiental, Depto. de Morfología. Escuela Nacional de Ciencias Biológicas, Ciudad de México, México.
authors.forename: Ramón-Gallegos
authors.initials: RG
authors.lastname: Eva
authors.affiliation: Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Campus Zacatenco, Unidad Profesional "Adolfo López Mateos", Calle Wilfrido Massieu esquina Cda. Manuel Stampa. C.P, 07738, Ciudad de México, México.
authors.forename: Robles-Ramírez María
authors.initials: RM
authors.lastname: Del Carmen
authors.affiliation: Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Campus Zacatenco, Unidad Profesional "Adolfo López Mateos", Calle Wilfrido Massieu esquina Cda. Manuel Stampa. C.P, 07738, Ciudad de México, México. rosalmora@hotmail.com.
authors.forename: Mora-Escobedo
authors.initials: ME
authors.lastname: Rosalva
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D018525:Germination; D006367:HeLa Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010455:Peptides; D010936:Plant Extracts; D011492:Protein Hydrolysates
keywords: Antioxidant; Antiproliferative effect; Peptide fractions; Soybean germination