title: Adipose Gene Expression Profile Changes With Lung Allograft Reperfusion.
abstract: Obesity is a risk factor for primary graft dysfunction (PGD), a form of lung injury resulting from ischemia-reperfusion after lung transplantation, but the impact of ischemia-reperfusion on adipose tissue is unknown. We evaluated differential gene expression in thoracic visceral adipose tissue (VAT) before and after lung reperfusion. Total RNA was isolated from thoracic VAT sampled from six subjects enrolled in the Lung Transplant Body Composition study before and after allograft reperfusion and quantified using the Human Gene 2.0 ST array. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed enrichment for genes involved in complement and coagulation cascades and Jak-STAT signaling pathways. Overall, 72 genes were upregulated and 56 genes were downregulated in the postreperfusion time compared with baseline. Long pentraxin-3, a gene and plasma protein previously associated with PGD, was the most upregulated gene (19.5-fold increase, p = 0.04). Fibronectin leucine-rich transmembrane protein-3, a gene associated with cell adhesion and receptor signaling, was the most downregulated gene (4.3-fold decrease, p = 0.04). Ischemia-reperfusion has a demonstrable impact on gene expression in visceral adipose tissue in our pilot study of nonobese, non-PGD lung transplant recipients. Future evaluation will focus on differential adipose tissue gene expression and the development of PGD after transplant.
authors.affiliation: Pulmonary, Allergy, and Critical Care Division, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Diamond
authors.affiliation: Division of Pulmonary, Allergy, and Critical Care Medicine, Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Selim
authors.initials: S
authors.lastname: Arcasoy
authors.affiliation: Division of Pulmonary, Allergy, and Critical Care Medicine, Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Jamiela A
authors.initials: JA
authors.lastname: McDonnough
authors.affiliation: Department of Surgery, Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Joshua R
authors.initials: JR
authors.lastname: Sonett
authors.affiliation: Department of Surgery, Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Matthew
authors.initials: M
authors.lastname: Bacchetta
authors.affiliation: Department of Surgery, Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Frank
authors.initials: F
authors.lastname: D'Ovidio
authors.affiliation: Division of Cardiovascular Surgery, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
authors.forename: Edward
authors.initials: E
authors.lastname: Cantu
authors.affiliation: Division of Cardiovascular Surgery, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
authors.forename: Christian A
authors.initials: CA
authors.lastname: Bermudez
authors.affiliation: Division of Pulmonary, Allergy, and Critical Care Medicine, Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Amika
authors.initials: A
authors.lastname: McBurnie
authors.affiliation: Pulmonary, Allergy, and Critical Care Division, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
authors.forename: Melanie
authors.initials: M
authors.lastname: Rushefski
authors.affiliation: Pulmonary, Allergy, and Critical Care Division, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
authors.forename: Laurel H
authors.initials: LH
authors.lastname: Kalman
authors.affiliation: Pulmonary, Allergy, and Critical Care Division, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Oyster
authors.affiliation: Pulmonary, Allergy, and Critical Care Division, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
authors.forename: Carly
authors.initials: C
authors.lastname: D'Errico
authors.affiliation: Division of Cardiovascular Surgery, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Division of Rheumatology, Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Jon T
authors.initials: JT
authors.lastname: Giles
authors.affiliation: Department of Medicine, Naomi Berrie Diabetes Center, Columbia University, New York, New York.
authors.forename: Anthony
authors.initials: A
authors.lastname: Ferrante
authors.affiliation: Division of Cardiology, Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Matthew
authors.initials: M
authors.lastname: Lippel
authors.affiliation: Department of Surgery, Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Gopal
authors.initials: G
authors.lastname: Singh
authors.affiliation: Division of Pulmonary, Allergy, and Critical Care Medicine, Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: David J
authors.initials: DJ
authors.lastname: Lederer
authors.affiliation: Pulmonary, Allergy, and Critical Care Division, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
authors.forename: Jason D
authors.initials: JD
authors.lastname: Christie
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D064591:Allografts; D015415:Biomarkers / Q000378:metabolism; D002097:C-Reactive Protein / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D016040:Lung Transplantation / Q000009:adverse effects*; D008297:Male; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D009765:Obesity / Q000503:physiopathology*; D010865:Pilot Projects; D055031:Primary Graft Dysfunction / Q000209:etiology* / Q000473:pathology; D011379:Prognosis; D011446:Prospective Studies; D015424:Reperfusion; D012307:Risk Factors; D000683:Serum Amyloid P-Component / Q000235:genetics*; D059467:Transcriptome; D000099153:Pentraxins
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D000683:Serum Amyloid P-Component; D000099153:Pentraxins; C404492:FLRT3 protein, human
keywords: genomics; ischemia reperfusion injury (IRI); lung (allograft) function/dysfunction; lung transplantation/pulmonology; translational research/science