title: Production of a new tetracyclic triterpene sulfate metabolite sambacide by solid-state cultivated Fusarium sambucinum B10.2 using potato as substrate.
abstract: The aim of this work is to explore integracide analogues from secondary metabolites of microorganisms. A new tetracyclic triterpene sulfate was produced by solid-state fermentation (SSF) with Fusarium sambucinum B10.2. The tetracyclic triterpene sulfate was identified as (3S,5R,10S,11S,12S,13R,17R,20R)-4,4-dimethylergosta-8,14,24-triene-3,11,12-triol-12-acetate, 3-sulfate on the basis of HRESIMS, NMR and electronic circular dichroism (ECD) spectra and named sambacide (1). The antibacterial and antifungal assays of sambacide (1) showed significant antibacterial activities against Staphylococcus aureus and Escherichia coli. The fermentation conditions including culture media, fermentation temperature and time, were optimized. And potato was selected as the fermentation substrate, 28°C was used as the fermentation temperature, and 20-days fermentation time was determined for F. sambucinum-SSF to produce sambacide (1) with a high yield of 19.04±0.82g/kg. This paper provides an efficient approach to produce the antibacterial and antifungal agent sambacide (1) in a very high yield.
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Dong
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, China.
authors.forename: Le
authors.initials: L
authors.lastname: Cai
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, China.
authors.forename: Xue-Jiao
authors.initials: XJ
authors.lastname: Li
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, China.
authors.forename: Rong-Ting
authors.initials: RT
authors.lastname: Duan
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shu
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, China.
authors.forename: Feng-Yun
authors.initials: FY
authors.lastname: Chen
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, China.
authors.forename: Jia-Peng
authors.initials: JP
authors.lastname: Wang
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, China. Electronic address: ztding@ynu.edu.cn.
authors.forename: Zhong-Tao
authors.initials: ZT
authors.lastname: Ding
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005285:Fermentation; D005670:Fusarium / Q000737:chemistry / Q000378:metabolism; D011198:Solanum tuberosum / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013431:Sulfates / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013752:Tetracycline / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D013431:Sulfates; D014315:Triterpenes; D013752:Tetracycline
keywords: Antibacterial activity; Antifungal activity; Fusarium sambucinum; Sambacide; Solid-state fermentation