title: Phthalide Derivatives with Anticoagulation Activities from Angelica sinensis.
abstract: Two new phthalide derivatives, angesinenolides A and B (1 and 2), were isolated from the roots of Angelica sinensis. Their structures were elucidated using HRMS, NMR, and X-ray crystallographic data. Compound 1 is the first example of a phthalide trimer presumably formed through two [2+2] cycloaddition reactions. Compound 2 is a unique dimeric phthalide with a peroxy bridge between C-3a and C-6. Both phthalides were evaluated for in vitro anticoagulation activities. Compound 1 reduced the level of fibrinogen (FIB). Compound 2 significantly extended thrombin time and activated partial thromboplastin time, as well as markedly reduced the content of FIB.
authors.affiliation: School of Pharmacy, Xinxiang Medical University , Xinxiang 453003, People's Republic of China.
authors.forename: Lai-Bin
authors.initials: LB
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Xinxiang Medical University , Xinxiang 453003, People's Republic of China.
authors.forename: Jie-Li
authors.initials: JL
authors.lastname: Lv
authors.affiliation: School of Pharmacy, Xinxiang Medical University , Xinxiang 453003, People's Republic of China.
authors.forename: Jun-Wei
authors.initials: JW
authors.lastname: Liu
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry*; D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005340:Fibrinogen / Q000032:analysis / Q000187:drug effects; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D013917:Thrombin / Q000032:analysis / Q000187:drug effects
chemical_list: D000925:Anticoagulants; D001572:Benzofurans; D004365:Drugs, Chinese Herbal; C000618151:angesinenolide A; C000618152:angesinenolide B; C082022:phthalide; D005340:Fibrinogen; D013917:Thrombin