title: Synergistic induction of cardiomyocyte differentiation from human bone marrow mesenchymal stem cells by interleukin 1β and 5-azacytidine.
abstract: Interleukin-1β (IL-1β) is a cytokine protein expressed by cardiomyocyte in myocardial damage-associated inflammation. Although several methods are currently available for treatment of heart diseases none of them are completely successful. Recently, stem cells have gained enormous attention and are expected to play a significant role for treating heart diseases. 5-Azacytidine (5-aza) has recently been found to cause stimulation of stem cells to differentiate into cardiomyocytes. Here we present the determination of whether IL-1β can induce the differentiation of mesenchymal stem cells (MSCs) to cardiomyocytes. MSCs were derived from bone marrow, propagated and then cultured in differentiation medium supplemented with 5-aza and IL-1β (at two levels, 5 and 10 ng/ml). After 21 days, total RNA was extracted and cDNA synthesis was carried out. Quantitative polymerase chain reaction (Q-PCR) was performed for detecting cardiac-specific markers. Besides, to investigate the expression of cardiac markers in protein levels, immunocytochemistry was done by specific antibodies. Ultimately, cardiac markers expression suggested that IL-1β and 5-aza synergistically induces the cardiomyocyte differentiation.
authors.forename: Safoura
authors.initials: S
authors.lastname: Khajeniazi
authors.forename: Mahin
authors.initials: M
authors.lastname: Solati
authors.forename: Yaghuob
authors.initials: Y
authors.lastname: Yazdani
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.forename: Anvarsadat
authors.initials: A
authors.lastname: Kianmehr
mesh_terms: D001374:Azacitidine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D018031:Connexin 43 / Q000235:genetics; D004357:Drug Synergism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology*; D020107:Troponin T / Q000378:metabolism
chemical_list: D018031:Connexin 43; D053583:Interleukin-1beta; D020107:Troponin T; D001374:Azacitidine