title: Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages.
abstract: A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. For initiation of experiments, fresh or frozen monocytes are cultured in flasks for 1 week with M-CSF to induce their differentiation into macrophages. Then, the macrophages can be harvested and seeded into culture wells at required cell densities for carrying out experiments. The use of defined numbers of macrophages rather than defined numbers of monocytes to initiate macrophage cultures for experiments yields macrophage cultures in which the desired cell density can be more consistently attained. Use of cryopreserved monocytes reduces dependency on donor availability and produces more homogeneous macrophage cultures.
authors.affiliation: Experimental Atherosclerosis Section, National Heart, Lung, and Blood Institute, National Institutes of Health.
authors.forename: Xueting
authors.initials: X
authors.lastname: Jin
authors.affiliation: Experimental Atherosclerosis Section, National Heart, Lung, and Blood Institute, National Institutes of Health; kruthh@nhlbi.nih.gov.
authors.forename: Howard S
authors.initials: HS
authors.lastname: Kruth
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor; D008264:Macrophages; D009000:Monocytes
chemical_list: D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor