title: Calcium dynamics change in degenerating cone photoreceptors.
abstract: Cone photoreceptors (cones) are essential for high-resolution daylight vision and colour perception. Loss of cones in hereditary retinal diseases has a dramatic impact on human vision. The mechanisms underlying cone death are poorly understood, and consequently, there are no treatments available. Previous studies suggest a central role for calcium (Ca2+) homeostasis deficits in photoreceptor degeneration; however, direct evidence for this is scarce and physiological measurements of Ca2+in degenerating mammalian cones are lacking.Here, we took advantage of the transgenic HR2.1:TN-XL mouse line that expresses a genetically encoded Ca2+biosensor exclusively in cones. We cross-bred this line with mouse models for primary ("cone photoreceptor function loss-1", cpfl1) and secondary ("retinal degeneration-1", rd1) cone degeneration, respectively, and assessed resting Ca2+levels and light-evoked Ca2+responses in cones using two-photon imaging. We found that Ca2+dynamics were altered in cpfl1 cones, showing higher noise and variable Ca2+levels, with significantly wider distribution than for wild-type and rd1 cones. Unexpectedly, up to 21% of cpfl1 cones still displayed light-evoked Ca2+responses, which were larger and slower than wild-type responses. In contrast, genetically intact rd1 cones were characterized by lower noise and complete lack of visual function.Our study demonstrates alterations in cone Ca2+dynamics in both primary and secondary cone degeneration. Our results are consistent with the view that higher (fluctuating) cone Ca2+levels are involved in photoreceptor cell death in primary (cpfl1) but not in secondary (rd1) cone degeneration. These findings may guide the future development of therapies targeting photoreceptor Ca2+homeostasis.
authors.affiliation: Institute for Ophthalmic Research.|Werner Reichardt Centre for Integrative Neuroscience.|Graduate School of Cellular & Molecular Neuroscience.
authors.forename: Manoj
authors.initials: M
authors.lastname: Kulkarni
authors.affiliation: Institute for Ophthalmic Research.
authors.forename: Dragana
authors.initials: D
authors.lastname: Trifunović
authors.affiliation: Institute for Ophthalmic Research.|Werner Reichardt Centre for Integrative Neuroscience.
authors.forename: Timm
authors.initials: T
authors.lastname: Schubert
authors.affiliation: Institute for Ophthalmic Research francois.paquet-durand@klinikum.uni-tuebingen.de(F.P-D.) thomas.euler@cin.uni-tuebingen.de(T.E.).|Werner Reichardt Centre for Integrative Neuroscience.|Bernstein Centre for Computational Neuroscience, University of Tübingen, Tübingen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Euler
authors.affiliation: Institute for Ophthalmic Research francois.paquet-durand@klinikum.uni-tuebingen.de(F.P-D.) thomas.euler@cin.uni-tuebingen.de(T.E.).
authors.forename: François
authors.initials: F
authors.lastname: Paquet-Durand
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D051379:Mice; D008822:Mice, Transgenic; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism*; D012162:Retinal Degeneration / Q000378:metabolism*
chemical_list: D002118:Calcium