title: Using SANS with Contrast-Matched Lipid Bicontinuous Cubic Phases To Determine the Location of Encapsulated Peptides, Proteins, and Other Biomolecules.
abstract: An understanding of the location of peptides, proteins, and other biomolecules within the bicontinuous cubic phase is crucial for understanding and evolving biological and biomedical applications of these hybrid biomolecule-lipid materials, including during in meso crystallization and drug delivery. While theoretical modeling has indicated that proteins and additive lipids might phase separate locally and adopt a preferred location in the cubic phase, this has never been experimentally confirmed. We have demonstrated that perfectly contrast-matched cubic phases in D2O can be studied using small-angle neutron scattering by mixing fully deuterated and hydrogenated lipid at an appropriate ratio. The model transmembrane peptide WALP21 showed no preferential location in the membrane of the diamond cubic phase of phytanoyl monoethanolamide and was not incorporated in the gyroid cubic phase. While deuteration had a small effect on the phase behavior of the cubic phase forming lipids, the changes did not significantly affect our results.
authors.affiliation: CSIRO Manufacturing , Clayton, Victoria 3168, Australia.
authors.forename: Leonie
authors.initials: L
authors.lastname: van 't Hag
authors.forename: Liliana
authors.initials: L
authors.lastname: de Campo
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Garvey
authors.affiliation: CSIRO Manufacturing , Clayton, Victoria 3168, Australia.
authors.forename: George C
authors.initials: GC
authors.lastname: Feast
authors.forename: Anna E
authors.initials: AE
authors.lastname: Leung
authors.forename: Nageshwar Rao
authors.initials: NR
authors.lastname: Yepuri
authors.forename: Robert
authors.initials: R
authors.lastname: Knott
authors.affiliation: School of Science, College of Science, Engineering and Health, RMIT University , Melbourne, Victoria 3001, Australia.
authors.forename: Tamar L
authors.initials: TL
authors.lastname: Greaves
authors.affiliation: School of Science, College of Science, Engineering and Health, RMIT University , Melbourne, Victoria 3001, Australia.
authors.forename: Nhiem
authors.initials: N
authors.lastname: Tran
authors.affiliation: The ARC Dairy Innovation Hub, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Sally L
authors.initials: SL
authors.lastname: Gras
authors.affiliation: CSIRO Manufacturing , Clayton, Victoria 3168, Australia.|School of Science, College of Science, Engineering and Health, RMIT University , Melbourne, Victoria 3001, Australia.
authors.forename: Calum J
authors.initials: CJ
authors.lastname: Drummond
authors.affiliation: School of Science, College of Science, Engineering and Health, RMIT University , Melbourne, Victoria 3001, Australia.
authors.forename: Charlotte E
authors.initials: CE
authors.lastname: Conn
mesh_terms: D002152:Calorimetry, Differential Scanning; D041961:Deuterium Exchange Measurement; D017666:Deuterium Oxide / Q000737:chemistry / Q000378:metabolism; D033363:Neutron Diffraction; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D053838:Scattering, Small Angle; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D010455:Peptides; D011506:Proteins; D017666:Deuterium Oxide