title: PHYLOGENY OF ANGIOSTRONGYLUS CANTONENSIS IN THAILAND BASED ON CYTOCHROME C OXIDASE SUBUNIT I GENE SEQUENCE.
abstract: Angiostrongylus cantonensis is an emerging infectious agent causing eosinophilic meningitis or meningoencephalitis in humans with clinical manifestation of severe headache. Molecular genetic studies on classification and phylogeny of A. cantonensis in Thailand are limited. This study surveyed A. cantonensis larvae prevalence in natural intermediate hosts across Thailand and analyzed their phylogenetic relationships. A total of 14,032 freshwater and land snails were collected from 19 provinces of Thailand. None of Filopaludina sp, Pomacea sp, and Cyclophorus sp were infected with Angiostrongylus larvae, whereas Achatina fulica, Cryptozona siamensis, and Megaustenia siamensis collected from Kalasin, Kamphaeng Phet, Phetchabun, Phitsanulok, and Tak Provinces were infected, with C. siamensis being the common intermediate host. Based on morphology, larvae isolated from 11 samples of these naturally infected snails preliminarily were identified as A. cantonensis. Comparison of partial nucleotide sequences of cytochrome c oxidase subunit I gene revealed that four sequences are identical to A. cantonensis haplotype ac4 from Bangkok and the other seven to that of A. cantonensis isolate AC Thai, indicating two independent lineages of A. cantonensis in Thailand.
authors.forename: Vitta
authors.initials: V
authors.lastname: Apichat
authors.forename: Srisongcram
authors.initials: S
authors.lastname: Narongrit
authors.forename: Thiproaj
authors.initials: T
authors.lastname: Jittranuch
authors.forename: Wongma
authors.initials: W
authors.lastname: Anucha
authors.forename: Polsut
authors.initials: P
authors.lastname: Wilaiwan
authors.forename: Fukruksa
authors.initials: F
authors.lastname: Chamaiporn
authors.forename: Yimthin
authors.initials: Y
authors.lastname: Thatcha
authors.forename: Mangkit
authors.initials: M
authors.lastname: Bandid
authors.forename: Thanwisai
authors.initials: T
authors.lastname: Aunchalee
authors.forename: Dekumyoy
authors.initials: D
authors.lastname: Paron
mesh_terms: D017185:Angiostrongylus cantonensis / Q000145:classification / Q000235:genetics; D000818:Animals; D018509:DNA, Helminth / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D006801:Humans; D007814:Larva; D010802:Phylogeny; D015995:Prevalence; D012908:Snails / Q000469:parasitology; D017206:Strongylida Infections / Q000453:epidemiology / Q000469:parasitology; D013785:Thailand / Q000453:epidemiology
chemical_list: D018509:DNA, Helminth; D003576:Electron Transport Complex IV