title: Acute Aortic Dissection Biomarkers Identified Using Isobaric Tags for Relative and Absolute Quantitation.
abstract: The purpose of this study was to evaluate the utility of potential serum biomarkers for acute aortic dissection (AAD) that were identified by isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approaches. Serum samples from 20 AAD patients and 20 healthy volunteers were analyzed using iTRAQ technology. Protein validation was performed using samples from 120 patients with chest pain. A total of 355 proteins were identified with the iTRAQ approach; 164 proteins reached the strict quantitative standard, and 125 proteins were increased or decreased more than 1.2-fold (64 and 61 proteins were up- and downregulated, resp.). Lumican, C-reactive protein (CRP), thrombospondin-1 (TSP-1), and D-dimer were selected as candidate biomarkers for the validation tests. Receiver operating characteristic (ROC) curves show that Lumican and D-dimer have diagnostic value (area under the curves [AUCs] 0.895 and 0.891, P < 0.05). For Lumican, the diagnostic sensitivity and specificity were 73.33% and 98.33%, while the corresponding values for D-dimer were 93.33% and 68.33%. For Lumican and D-dimer AAD combined diagnosis, the sensitivity and specificity were 88.33% and 95%, respectively. In conclusion, Lumican has good specificity and D-dimer has good sensitivity for the diagnosis of AAD, while the combined detection of D-dimer and Lumican has better diagnostic value.
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Ziya
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Chenling
authors.identifier: 0000-0003-0528-2038
authors.initials: C
authors.lastname: Yao
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Guorong
authors.initials: G
authors.lastname: Gu
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Fan
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Luan
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Zhengang
authors.initials: Z
authors.lastname: Tao
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Zhen-Ju
authors.initials: ZJ
authors.lastname: Song
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Tong
authors.affiliation: Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Haojun
authors.initials: H
authors.lastname: Wang
mesh_terms: D000368:Aged; D000784:Aortic Dissection / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D001011:Aorta / Q000378:metabolism / Q000503:physiopathology*; D015415:Biomarkers / Q000097:blood*; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D015536:Down-Regulation; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D011237:Predictive Value of Tests; D040901:Proteomics; D012372:ROC Curve; D019700:Thrombospondin 1 / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D005338:Fibrin Fibrinogen Degradation Products; D019700:Thrombospondin 1; C036309:fibrin fragment D; C000611726:thrombospondin-1, human; D002097:C-Reactive Protein