title: WISP1 Is Increased in Intestinal Mucosa and Contributes to Inflammatory Cascades in Inflammatory Bowel Disease.
abstract: Inflammatory bowel disease (IBD) is mainly characterized by intestinal tissue damage, which is caused by excessive autoimmune responses poorly controlled by corresponding regulatory mechanisms. WISP1, which belongs to the CCN protein family, is a secreted matricellular protein regulating several inflammatory pathways, such as Wnt/β-catenin pathway, and has been reported in several diseases including cancer. Here we examined the expression, regulatory mechanisms, and functions of WISP1 in IBD. WISP1 mRNA and protein expression was upregulated in colonic biopsies and lamina propria mononuclear cells (LPMC) of IBD patients compared with those of healthy controls. Tumor necrosis factor- (TNF-) α induced WISP1 expression in LPMC from healthy controls. Consistently, WISP1 mRNA expression was downregulated in colonic biopsies from IBD patients who had achieved clinical remission with infliximab (IFX). Furthermore, WISP1 expression was also found to be increased in colons from 2,4,6-trinitrobenzenesulfonic acid- (TNBS-) induced mice compared with those from control mice. Further studies confirmed that administration of rWISP1 could aggravate TNBS-induced colitis in vivo. Therefore, we concluded that WISP1 is increased in IBD and contributes to the proinflammatory cascades in the gut.
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Liang
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Xinzhi
authors.initials: X
authors.lastname: Shan
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Qinghui
authors.initials: Q
authors.lastname: Niu
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Zibin
authors.initials: Z
authors.lastname: Tian
mesh_terms: D000328:Adult; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D055511:CCN Intercellular Signaling Proteins / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D060449:Wnt Signaling Pathway
chemical_list: D015415:Biomarkers; D055511:CCN Intercellular Signaling Proteins; C116396:CCN4 protein, human; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha