title: Vertical variation of bacterial community in Nam Co, a large stratified lake in central Tibetan Plateau.
abstract: We assessed the vertical variation of bacterial abundance, diversity and community composition in deep, high-altitude (4730 m above sea level) Lake Nam Co. The bacterial abundance reduced with increasing depth, while bacterial diversity increased in the deeper bottom water. Pyrosequencing revealed that the bacterial community in the lake showed a pronounced vertical shift: the Bacteroidetes and Proteobacteria had relatively higher abundance in the epilimnion and metalimnion; the Firmicutes group dominated in the metalimnion; the Actinobacteria and Cyanobacteria mainly occupied the metalimnion and hypolimnion. Water temperature, conductivity, photosynthetically active radiation, and chlorophyll a were significantly correlated with the bacterial communities. These results indicated that the bacterial communities appeared to have an apparent vertical structure under lake stratification.
authors.affiliation: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, 100101, Beijing, China.
authors.forename: Keshao
authors.initials: K
authors.lastname: Liu
authors.affiliation: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, 100101, Beijing, China. yqliu@itpcas.ac.cn.|CAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, 100101, Beijing, China. yqliu@itpcas.ac.cn.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China.
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, 100101, Beijing, China.|CAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, 100101, Beijing, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, 100101, Beijing, China.|CAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, 100101, Beijing, China.
authors.forename: Junbo
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, 361021, Xiamen, China.
authors.forename: Anyi
authors.initials: A
authors.lastname: Hu
authors.affiliation: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, 100101, Beijing, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Liu
mesh_terms: D000531:Altitude; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D044822:Biodiversity; D060106:Lakes / Q000382:microbiology*; D058889:Molecular Typing; D018609:Tibet; D014780:Viruses / Q000145:classification / Q000302:isolation & purification; D014871:Water Microbiology
keywords: Bacterial community composition; Lake Nam Co; Pyrosequencing; Tibetan Plateau; Vertical shift