title: Efficacy of a collagen-based dressing in an animal model of delayed wound healing.
abstract: OBJECTIVE
  The aim of this study was to evaluate in vitro and in vivo the efficacy of GBT013, a collagen-based dressing, for the treatment of chronic wounds, in a db/db mouse model of diabetes.
  
  
  METHODS
  Macroscopic and histologic analyses of db/db mice wound healing with GBT013 or saline gauze were assessed. The mRNA expression and the proliferation of dermal fibroblast were investigated. Matrix metalloproteinases (MMP)-2 and MMP-9 activities were quantified.
  
  
  RESULTS
  In db/db mice, GBT013 improves wound epithelialisation when compared with saline gauze. Histological analysis of scar tissue also shows an enhancement of remodelling associated with no sign of acute inflammation. In addition, GBT013 significantly decreases interleukin (IL)-6 and IL-8, significantly increases tissue inhibitors of metalloproteinases (TIMP)-1 and TIMP-2 fibroblast mRNA expression and significantly reduces in vitro MMP-2 and MMP-9 enzymatic activities. Moreover, GBT013 allows cell growth inside the matrix and stimulates proliferation of human dermal fibroblast.
  
  
  CONCLUSIONS
  By contributing to restore MMPs/TIMPs balance, GBT013 may function in all key stages of wound healing, such as inflammation, proliferation and tissue remodelling, and ultimately may provide a favourable environment for skin repair.
  
  
  BACKGROUND
  This work was supported by Genbiotech, the R&D subsidiary of Laboratoires Genévrier, a pharmaceutical company.
authors.affiliation: Department of Research and Development, Genbiotech, 06600 Antibes, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Guillemin
authors.affiliation: Department of Research and Development, Genbiotech, 06600 Antibes, France.
authors.forename: D
authors.initials: D
authors.lastname: Le Broc
authors.affiliation: Department of Research and Development, Genbiotech, 06600 Antibes, France.
authors.forename: C
authors.initials: C
authors.lastname: Ségalen
authors.affiliation: Department of Research and Development, Genbiotech, 06600 Antibes, France.
authors.forename: E
authors.initials: E
authors.lastname: Kurkdjian
authors.affiliation: Department of Research and Development, Genbiotech, 06600 Antibes, France.
authors.forename: J N
authors.initials: JN
authors.lastname: Gouze
mesh_terms: D000818:Animals; D001458:Bandages; D003094:Collagen / Q000627:therapeutic use*; D048909:Diabetes Complications / Q000628:therapy*; D005347:Fibroblasts / Q000502:physiology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051379:Mice; D023421:Models, Animal; D012867:Skin / Q000293:injuries* / Q000503:physiopathology*; D012965:Sodium Chloride; D014945:Wound Healing / Q000502:physiology*
chemical_list: D012965:Sodium Chloride; D003094:Collagen; D020778:Matrix Metalloproteinase 2
keywords: collagen-scaffold; diabetic foot ulcer; epithelialisation; matrix metalloproteinases; tissue remodelling