title: Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.
abstract: During metazoan development, the temporal pattern of morphogen signaling is critical for organizing cell fates in space and time. Yet, tools for temporally controlling morphogen signaling within the embryo are still scarce. Here, we developed a photoactivatable Nodal receptor to determine how the temporal pattern of Nodal signaling affects cell fate specification during zebrafish gastrulation. By using this receptor to manipulate the duration of Nodal signaling in vivo by light, we show that extended Nodal signaling within the organizer promotes prechordal plate specification and suppresses endoderm differentiation. Endoderm differentiation is suppressed by extended Nodal signaling inducing expression of the transcriptional repressor goosecoid (gsc) in prechordal plate progenitors, which in turn restrains Nodal signaling from upregulating the endoderm differentiation gene sox17 within these cells. Thus, optogenetic manipulation of Nodal signaling identifies a critical role of Nodal signaling duration for organizer cell fate specification during gastrulation.
authors.affiliation: Laboratory of Developmental Biology, Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sako
authors.affiliation: Indian Institute of Science, Education and Research, Pune 411008, India.
authors.forename: Saurabh J
authors.initials: SJ
authors.lastname: Pradhan
authors.affiliation: Laboratory of Developmental Biology, Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Barone
authors.affiliation: Laboratory of Synthetic Physiology, Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors.forename: Álvaro
authors.initials: Á
authors.lastname: Inglés-Prieto
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, 72076 Tübingen, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Müller
authors.affiliation: Laboratory of Developmental Biology, Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors.forename: Verena
authors.initials: V
authors.lastname: Ruprecht
authors.affiliation: Laboratory of Developmental Biology, Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors.forename: Daniel
authors.initials: D
authors.lastname: Čapek
authors.affiliation: Indian Institute of Science, Education and Research, Pune 411008, India.
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Galande
authors.affiliation: Laboratory of Synthetic Physiology, Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria. Electronic address: harald@ist.ac.at.
authors.forename: Harald
authors.initials: H
authors.lastname: Janovjak
authors.affiliation: Laboratory of Developmental Biology, Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria. Electronic address: heisenberg@ist.ac.at.
authors.forename: Carl-Philipp
authors.initials: CP
authors.lastname: Heisenberg
mesh_terms: D000818:Animals; D001483:Base Sequence; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000502:physiology; D004707:Endoderm / Q000378:metabolism / Q000502:physiology; D054262:Gastrulation / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D009024:Morphogenesis / Q000235:genetics / Q000502:physiology; D055457:Nodal Protein / Q000378:metabolism*; D062308:Optogenetics / Q000379:methods; D055760:SOXF Transcription Factors / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D055457:Nodal Protein; D055760:SOXF Transcription Factors; D029961:Zebrafish Proteins; C491603:sox17 protein, zebrafish