title: Protective effect of Gallic acid on doxorubicin-induced testicular and epididymal toxicity.
abstract: The effect of Gallic acid (GA) on doxorubicin (DOX)-induced testicular and epididymal toxicity was investigated in experimental rat model. The rats were randomly divided into six groups of 10 animals per group. Rats in group A received clean tap water ad libitum. Rats in group B were administered DOX intraperitoneally at 15 mg/kg on the eighth day of the experiment. Animals in groups C and D received 60 and 120 mg/kg GA orally for 7 days with 15 mg/kg DOX on the eighth day. Rats in groups E and F received 60 and 120 mg/kg GA alone orally for 7 days. The animals were sacrificed 24 hr after the last administration. DOX administration led to a significant (p < 0.05) increase in hydrogen peroxide and malondialdehyde levels with significant reduction in antioxidant enzymes and reduced glutathione levels. DOX administration also led to a significant increase in total sperm abnormalities and prolactin together with a significant decrease in testosterone levels. Immunohistochemistry revealed higher expressions of caspase 3 in the testicular tissues of rats that received DOX alone. Together, pre-treatment with GA attenuated markers of oxidative stress, reversed sperm abnormality and ameliorated the observed aberration in plasma testosterone and prolactin levels.
authors.affiliation: Department of Surgery and Reproduction, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: M J
authors.initials: MJ
authors.lastname: Olusoji
authors.affiliation: Department of Surgery and Reproduction, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: O M
authors.initials: OM
authors.lastname: Oyeyemi
authors.affiliation: Department of Veterinary Biochemistry, Faculty of Veterinary Medicine, University of Benin, Benin, Nigeria.
authors.forename: E R
authors.initials: ER
authors.lastname: Asenuga
authors.affiliation: Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: T O
authors.initials: TO
authors.lastname: Omobowale
authors.affiliation: Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: O L
authors.initials: OL
authors.lastname: Ajayi
authors.affiliation: Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria.
authors.forename: A A
authors.initials: AA
authors.lastname: Oyagbemi
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D004195:Disease Models, Animal; D004317:Doxorubicin / Q000009:adverse effects*; D004822:Epididymis / Q000187:drug effects*; D005707:Gallic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D005978:Glutathione / Q000032:analysis; D006861:Hydrogen Peroxide / Q000032:analysis; D007274:Injections, Intraperitoneal; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects; D011388:Prolactin / Q000097:blood; D011897:Random Allocation; D051381:Rats; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000097:blood
chemical_list: D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D013739:Testosterone; D008315:Malondialdehyde; D005707:Gallic Acid; D004317:Doxorubicin; D011388:Prolactin; D006861:Hydrogen Peroxide; D053148:Caspase 3; D005978:Glutathione
keywords: Gallic acid; apoptosis; doxorubicin; oxidative stress; reproductive toxicity