title: Mepenzolate bromide promotes diabetic wound healing by modulating inflammation and oxidative stress.
abstract: Diabetic wounds are characterized by persistent inflammation and the excessive production of reactive oxygen species, thus resulting in impaired wound healing. Mepenzolate bromide, which was originally used to treat gastrointestinal disorders in clinical settings, has recently been shown to display beneficial effects in chronic obstructive pulmonary disease and pulmonary fibrosis of a mouse model by inhibiting inflammatory responses and reducing oxidative stress. However,the role of mepenzolate bromide in diabetic wound healing is still unclear. In this study, full-thickness excisional skin wounds were created on the backs of db/db mice, and mepenzolate bromide was topically applied to the wound bed. We found that mepenzolate bromide significantly promoted diabetic wound healing by measuring wound closure rate and histomorphometric analyses. Further studies showed that inflammation was inhibited by assessing the number of macrophages and levels of pro-inflammatory cytokines and pro-healing cytokines in the wounds. Furthermore, oxidative stress was reduced by monitoring the levels of MDA and H2O2 and the activities of glutathione peroxidase and catalase in the wounds. These results demonstrated the potential application of mepenzolate bromide for treating diabetic ulcers and other chronic wounds in clinics.
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Xingtong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Shizhao
authors.initials: S
authors.lastname: Ji
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Song
authors.initials: S
authors.lastname: Tian
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wu
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Luo
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: He
authors.initials: H
authors.lastname: Fang
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Wu
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Shichu
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Zhaofan
authors.initials: Z
authors.lastname: Xia
keywords: Mepenzolate bromide; diabetes; inflammation; oxidative stress; wound healing