title: Values of SP-A protein in the nasal mucosa.
abstract: The paranasal sinus epithelium is exposed to the environment and therefore to a variety of biological, chemical and mechanical insults. Surfactant protein A (SP-A) is a 34-36 kD pulmonary surfactant-associated protein that appears to play an important role in mammalian first-line host defence. Recent studies have reported the possibility of local production of SP-A in the extrapulmonary organs and tissues of the human body. However, the presence of SP-A in the human paranasal sinus mucosa is not well known. The purpose of this study was to investigate the expression of SP-A protein in human turbinate mucosa and to compare the expression of SP-A mRNA in normal turbinate mucosa and turbinate mucosa of chronic rhinosinusitis patients. Reverse transcriptase polymerase chain reaction was used to detect SP-A mRNA. Student's t test was used for statistical comparison of the SP-A/GAPDH-mRNA ratio (GAPDH: glycerinaldehyde-3-phosphate dehydrogenase) of cases and controls. We found expression of SP-A mRNA in mucosa lining the inferior turbinates of healthy patients and its up-regulation in mucosa lining the inferior turbinates of patients with chronic rhinosinusitis. These results may provide targets for new therapies for chronic rhinosinusitis.
authors.affiliation: Department of Otorhinolaryngology, University of Siena, Siena, Italy. d.passali@virgilio.it.
authors.forename: D
authors.initials: D
authors.lastname: Passali
authors.affiliation: Department of Otorhinolaryngology, University of Siena, Siena, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Astore
authors.affiliation: Department of Otorhinolaryngology, "Misericordia" Hospital of Grosseto, Grosseto, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Boccuzzi
authors.affiliation: Department of Otorhinolaryngology, University of Siena, Siena, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Loglisci
authors.affiliation: Department of Medical Biotechnologies, University of Siena, Siena, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Cannatelli
authors.affiliation: Department of Otorhinolaryngology, "Catholic University of Sacred Hearth", Rome, Italy.
authors.forename: G C
authors.initials: GC
authors.lastname: Passali
authors.affiliation: Department of Otorhinolaryngology, University of Siena, Siena, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Bellussi
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009093:Mucus / Q000737:chemistry*; D009297:Nasal Mucosa / Q000737:chemistry*; D015508:Nasal Obstruction / Q000175:diagnosis / Q000378:metabolism*; D037662:Pulmonary Surfactant-Associated Protein A / Q000032:analysis*; D015203:Reproducibility of Results; D012220:Rhinitis / Q000175:diagnosis / Q000378:metabolism*; D012680:Sensitivity and Specificity; D012852:Sinusitis / Q000175:diagnosis / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D037662:Pulmonary Surfactant-Associated Protein A
keywords: Paranasal sinuses; Respiratory tract; Rhinitis; Sinusitis; Surfactant protein A