title: Relationship Between Gonadotropin-Releasing Hormone/Gonadotropin-Releasing Hormone Receptor Signaling and Progesterone Receptors in Human Trophoblasts.
abstract: OBJECTIVE
  To investigate the relationship between gonadotropin-releasing hormone (GnRH)/gonadotropin-releasing hormone receptor (GnRHR) signaling and progesterone receptors (PGRs).
  
  
  METHODS
  HTR-8/SV neo cells and JEG-3 cells were incubated for 24 hours with GnRH-I or -II. The expression of PGRs was measured by quantitative real-time reverse transcriptase-polymerase chain reaction and Western blotting.
  
  
  RESULTS
  In both cells lines after incubation with 1-1,000 nM/L GnRH-I or -II the ratio of PGR-B to PGR-AB mRNA level did not differ in any of the groups as compared to the control. Relatively higher expression of PGR-B than PGR-A mRNA and expression of only PGR-B protein without PGR-A protein were observed in all specimens. There were no significant differences in the expression of PGR-B protein in each group when compared to the control group after incubation with GnRH-I or -II at 0.001-1,000 mM/L in both cell lines.
  
  
  CONCLUSIONS
  PGRs are expressed regardless of GnRH/GnRHR signaling in human trophoblasts, in which PGR-B expression is more dominant than PGR-A expression.
authors.forename: Bang Hyun
authors.initials: BH
authors.lastname: Lee
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Tae Chul
authors.initials: TC
authors.lastname: Park
authors.forename: Hee Joong
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D011498:Protein Precursors / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011966:Receptors, LHRH / Q000187:drug effects* / Q000378:metabolism; D011980:Receptors, Progesterone / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; D011966:Receptors, LHRH; D011980:Receptors, Progesterone; C101118:progesterone receptor A; C097314:progesterone receptor B; C050866:progonadoliberin I; D007987:Gonadotropin-Releasing Hormone; C047717:LHRH, His(5)-Trp(7)-Tyr(8)-