title: Transcriptomic Profiling of Adipose Tissue in Obese Women in Response to Acupuncture Catgut Embedding Therapy with Moxibustion.
abstract: OBJECTIVE
  Complementary and alternative medicine, such as Traditional Chinese Medicine, represents an efficient therapeutic option for obesity control. It was previously reported that acupuncture catgut embedding therapy (ACET) with moxibustion reduces body weight and reverts insulin resistance in obese women. This study aimed to evidence changes in adipokines and gene expression in adipose tissue that could explain the effects of ACET with moxibustion.
  
  
  METHODS
  Overweight/obese women were treated with ACET with moxibustion or sham acupuncture as control. Peripheral blood samples and fat biopsies were taken before and after intervention. Circulating adipokines (leptin, adiponectin, tumor necrosis factor alpha, and resistin) were quantified by enzyme-linked immunosorbent assay. Gene expression in adipose tissue was determined by cDNA microarray assays and assessed by quantitative reverse transcription real-time polymerase chain reaction.
  
  
  RESULTS
  ACET with moxibustion did not modify circulating adipokines levels. However, correlations with anthropometric and biochemical parameters were affected. Interestingly, transcriptional changes in adipose tissue revealed the modulation of genes participating in homeostasis control, lipid metabolism, olfactory transduction, and gamma-aminobutyric acid signaling pathway.
  
  
  CONCLUSIONS
  The effects of ACET with moxibustion on body weight and insulin resistance were associated with the regulation of biochemical events that are altered in obesity.
authors.affiliation: 1 Biotechnology Program, ENMH, Instituto Politécnico Nacional , Mexico City, Mexico .
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Garcia-Vivas
authors.affiliation: 2 Biotechnology Program, CIIDIR, Instituto Politécnico Nacional , Durango City, Durango, Mexico .
authors.forename: Carlos
authors.initials: C
authors.lastname: Galaviz-Hernandez
authors.affiliation: 3 Oncogenomics Laboratory, National Institute of Cancerology , Mexico City, Mexico .
authors.forename: Jorge
authors.initials: J
authors.lastname: Fernandez-Retana
authors.affiliation: 3 Oncogenomics Laboratory, National Institute of Cancerology , Mexico City, Mexico .
authors.forename: Abraham
authors.initials: A
authors.lastname: Pedroza-Torres
authors.affiliation: 3 Oncogenomics Laboratory, National Institute of Cancerology , Mexico City, Mexico .
authors.forename: Carlos
authors.initials: C
authors.lastname: Perez-Plasencia
authors.affiliation: 4 Genomics Sciences Program, Universidad Autónoma de la Ciudad de México , Mexico City, Mexico .
authors.forename: Cesar
authors.initials: C
authors.lastname: Lopez-Camarillo
authors.affiliation: 1 Biotechnology Program, ENMH, Instituto Politécnico Nacional , Mexico City, Mexico .|5 Molecular Biomedicine Program, ENMH, Instituto Politécnico Nacional , Mexico City, Mexico .
authors.forename: Laurence A
authors.initials: LA
authors.lastname: Marchat
mesh_terms: D015670:Acupuncture Therapy; D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism*; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D009071:Moxibustion; D009765:Obesity / Q000378:metabolism / Q000628:therapy*; D050177:Overweight / Q000378:metabolism / Q000628:therapy; D016133:Polymerase Chain Reaction; D059467:Transcriptome / Q000235:genetics*