title: Optimization of PEG-based extraction of polysaccharides from Dendrobium nobile Lindl. and bioactivity study.
abstract: Polyethylene glycol (PEG) as a green solvent was employed to extract polysaccharide. The optimal conditions for PEG-based ultrasonic extraction of Dendrobium nobile Lindl. polysaccharide (JCP) were determined by response surface methodology. Under the optimal conditions: extraction temperature of 58.5°C; ultrasound power of 193W, and the concentration of polyethylene glycol-200 (PEG-200) solution of 45%, the highest JCP yield was obtained as 15.23±0.57%, which was close to the predicted yield, 15.57%. UV and FT-IR analysis revealed the general characteristic absorption peaks of both JCP with water extraction (JCPw) and PEG-200 solvent extraction (JCPp). Thermal analysis of both JCPs was performed with Thermal Gravimetric Analyzer (TGA) and Differential Scanning Calorimeter (DSC). Antioxidant activities of two polysaccharides were also compared and no significant difference in vitro was obtained.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, PR China. Electronic address: hxwang@jiangnan.edu.cn.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, PR China.
authors.forename: ChaoYang
authors.initials: C
authors.lastname: Ma
authors.affiliation: GuoLi Dendrobium nobile Company Limited, ChiShui 564700, PR China.
authors.forename: GuoHua
authors.initials: G
authors.lastname: He
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Md Ramim Tanver
authors.initials: MR
authors.lastname: Rahman
mesh_terms: D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D031667:Dendrobium / Q000737:chemistry*; D005163:Factor Analysis, Statistical; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification*; D059625:Liquid-Liquid Extraction / Q000295:instrumentation / Q000379:methods*; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D013010:Sonication; D014867:Water / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D011134:Polysaccharides; D012997:Solvents; D014867:Water; D011092:Polyethylene Glycols; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant activity; Dendrobium nobile; Optimization; PEG; Polysaccharide; Response surface methodology