title: Long-term healing of mildly cross-linked decellularized bovine pericardial aortic patch.
abstract: Glutaraldehyde treated bovine pericardium is extensively used in cardiovascular surgery. However, frequent occurrence of failure modes, such as calcification and structural failure, has hard pressed the need for finding an alternate technology. Decellularized bovine pericardium is an emerging technology. Mildly cross-linked decellularized bovine pericardium promotes positive remodeling with insignificant calcification and acute inflammation. In the present study, mildly cross-linked decellularized bovine pericardium was evaluated as a cardiovascular patch by studying mechanical strength as well as graft remodeling, resistance to calcific degeneration and inflammatory response using long duration porcine aortic implantation. It was observed that decellularized bovine pericardium, although thinner and less elastic had equivalent tensile properties such as tensile strength and stiffness when compared to commercially available glutaraldehyde-treated bovine pericardium. It showed the potential for site appropriate remodeling evidenced by host cell incorporation, thinner neointima, graft degradation, and neocollagenisation making it suitable for vascular patch application, whereas glutaraldehyde-treated pericardium failed to integrate with host tissue through timely degradation and host cell incorporation or neocollagenization. Conversely, it elicited persistent acute inflammation and produced calcification. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2145-2152, 2017.
authors.affiliation: Division of In vivo Models and Testing, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
authors.forename: P R
authors.initials: PR
authors.lastname: Umashankar
authors.affiliation: Histopathology Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
authors.forename: A
authors.initials: A
authors.lastname: Sabareeswaran
authors.affiliation: Division of In vivo Models and Testing, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
authors.forename: Sachin J
authors.initials: SJ
authors.lastname: Shenoy
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D002417:Cattle; D005109:Extracellular Matrix / Q000737:chemistry / Q000637:transplantation; D005260:Female; D005976:Glutaral / Q000737:chemistry; D008297:Male; D058426:Neointima; D010496:Pericardium / Q000737:chemistry*; D013552:Swine
chemical_list: D005976:Glutaral
keywords: aortic patch; decellularised bovine pericardium; long-term healing