title: Inflammatory response study of gellan gum impregnated duck's feet derived collagen sponges.
abstract: Tissue engineered biomaterials have biodegradable and biocompatible properties. In this study, we have fabricated sponges using duck's feet derived collagen (DC) and gellan gum (GG), and further studied its inflammatory responses. The as-prepared duck's feet DC/GG sponges showed the possibility of application as a tissue engineering material through in vitro and in vivo experiments. The physical and chemical properties of sponges were characterized by compression strength, porosity, and scanning electron microscopy, etc. In vitro cell viability were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. An inflammatory response was studied after seeding RAW264.7 cells on as-fabricated sponges using reverse transcriptase-polymerase chain reaction. In vivo studies were carried out by implanting in subcutaneous nude mouse followed by extraction, histological staining. Collectively, superior results were showed by DC/GG sponges than GG sponge in terms of physical property and cell proliferation and thus can be considered as a potential candidate for future tissue engineering applications.
authors.affiliation: a Department of BIN Fusion Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center , Chonbuk National University , Jeonju , Republic of Korea.
authors.forename: Jeong Eun
authors.initials: JE
authors.lastname: Song
authors.affiliation: a Department of BIN Fusion Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center , Chonbuk National University , Jeonju , Republic of Korea.
authors.forename: Seon Eui
authors.initials: SE
authors.lastname: Lee
authors.affiliation: a Department of BIN Fusion Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center , Chonbuk National University , Jeonju , Republic of Korea.
authors.forename: Se Rom
authors.initials: SR
authors.lastname: Cha
authors.affiliation: a Department of BIN Fusion Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center , Chonbuk National University , Jeonju , Republic of Korea.
authors.forename: Na Keum
authors.initials: NK
authors.lastname: Jang
authors.affiliation: a Department of BIN Fusion Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center , Chonbuk National University , Jeonju , Republic of Korea.
authors.forename: Nirmalya
authors.initials: N
authors.lastname: Tripathy
authors.affiliation: b 3B's Research Group - Biomaterials, Biodegradables and Biomimetics , University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , Guimaraes , Portugal.
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
authors.affiliation: a Department of BIN Fusion Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center , Chonbuk National University , Jeonju , Republic of Korea.
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000009:adverse effects* / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000009:adverse effects* / Q000737:chemistry* / Q000494:pharmacology; D004372:Ducks; D005528:Foot; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D023822:Tissue Engineering
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D011135:Polysaccharides, Bacterial; C048288:gellan gum; D003094:Collagen
keywords: Gellan gum; collagen; inflammatory responses; sponge; tissue regeneration