title: FTIR assay method for UV inactive drug carisoprodol and identification of degradants by RP-HPLC and ESI-MS.
abstract: A new method of analysis has been developed for UV inactive drug carisoprodol using FTIR spectroscopy. These methods were validated for various parameters according to ICH guidelines. The proposed method has also been successfully applied for the determination of the drug concentration in a tablet formulation. The method proved to be accurate (mean percentage recovery between 95 and 105%), precise and reproducible (relative standard deviation<2%), while being simple, economical and less time consuming than other methods and can be used for routine estimation of carisoprodol in the pharmaceutical industry. The developed method also implicates its utility for other UV inactive substances. The stability of the drug under various stress conditions was studied and the drug was found to be particularly susceptible to alkaline hydrolysis. Degradation products of the alkaline hydrolysis were detected by RP-HPLC and tentatively identified by ESI-MS.
authors.affiliation: SPP School of Pharmacy and Technology Management, SVKM's NMIMS, Mumbai 400056, India. Electronic address: pratap.aacharya@gmail.com.
authors.forename: Pratap Chandra
authors.initials: PC
authors.lastname: Acharya
authors.affiliation: SPP School of Pharmacy and Technology Management, SVKM's NMIMS, Mumbai 400056, India.
authors.forename: Ruqaiya
authors.initials: R
authors.lastname: Vasi
authors.affiliation: SPP School of Pharmacy and Technology Management, SVKM's NMIMS, Mumbai 400056, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Suares
mesh_terms: D002328:Carisoprodol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D004355:Drug Stability; D006868:Hydrolysis; D009125:Muscle Relaxants, Central / Q000032:analysis*; D010782:Photolysis; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000191:economics / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000191:economics / Q000379:methods*; D013607:Tablets; D014466:Ultraviolet Rays
chemical_list: D009125:Muscle Relaxants, Central; D013607:Tablets; D002328:Carisoprodol
keywords: Carisoprodol quantitative estimation; Degradant identification; FTIR method development; Forced degradation; UV inactive drug