title: The proteome of higher plant mitochondria.
abstract: Plant mitochondria perform a wide range of functions in the plant cell ranging from providing energy and metabolic intermediates, via coenzyme biosynthesis and their own biogenesis to retrograde signaling and programmed cell death. To perform these functions, they contain a proteome of >2000 different proteins expressed in some cells under some conditions. The vast majority of these proteins are imported, in many cases by a dedicated protein import machinery. Recent proteomic studies have identified about 1000 different proteins in both Arabidopsis and potato mitochondria, but even for energy-related proteins, the most well-studied functional protein group in mitochondria, <75% of the proteins are recognized as mitochondrial by even one of six of the most widely used prediction algorithms. The mitochondrial proteomes contain proteins representing a wide range of different functions. Some protein groups, like energy-related proteins, membrane transporters, and de novo fatty acid synthesis, appear to be well covered by the proteome, while others like RNA metabolism appear to be poorly covered possibly because of low abundance. The proteomic studies have improved our understanding of basic mitochondrial functions, have led to the discovery of new mitochondrial metabolic pathways and are helping us towards appreciating the dynamic role of the mitochondria in the responses of the plant cell to biotic and abiotic stress.
authors.affiliation: Biostatistics and Bioinformatics Division, Yenepoya Research Center, Yenepoya University, Mangalore 575018, India.
authors.forename: R S P
authors.initials: RS
authors.lastname: Rao
authors.affiliation: Institute of Biology, Department of Plant Biology, University of Campinas, Cidade Universitária Zeferino Vaz - Barão Geraldo, Campinas CEP: 13083-970, São Paulo, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Salvato
authors.affiliation: Institut für Pflanzengenetik, Leibniz Universität Hannover, Herrenhäuser Str. 2, DE-30419 Hannover, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Thal
authors.affiliation: Institut für Pflanzengenetik, Leibniz Universität Hannover, Herrenhäuser Str. 2, DE-30419 Hannover, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Eubel
authors.affiliation: Department of Biochemistry, University of Missouri-Columbia, Christopher S. Bond Life Sciences Center, Columbia, MO 65211, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Thelen
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, DK-4200 Slagelse, Denmark. Electronic address: ian.max.moller@mbg.au.dk.
authors.forename: I M
authors.initials: IM
authors.lastname: Møller
mesh_terms: D019295:Computational Biology; D013058:Mass Spectrometry; D008928:Mitochondria / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis*; D010944:Plants / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: (Plant) mitochondria; Localization prediction programs; Proteomics