title: Canola engineered with a microalgal polyketide synthase-like system produces oil enriched in docosahexaenoic acid.
abstract: Dietary omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5) are usually derived from marine fish. Although production of both EPA and DHA has been engineered into land plants, including Arabidopsis, Camelina sativa and Brassica juncea, neither has been produced in commercially relevant amounts in a widely grown crop. We report expression of a microalgal polyketide synthase-like PUFA synthase system, comprising three multidomain polypeptides and an accessory enzyme, in canola (Brassica napus) seeds. This transgenic enzyme system is expressed in the cytoplasm, and synthesizes DHA and EPA de novo from malonyl-CoA without substantially altering plastidial fatty acid production. Furthermore, there is no significant impact of DHA and EPA production on seed yield in either the greenhouse or the field. Canola oil processed from field-grown grain contains 3.7% DHA and 0.7% EPA, and can provide more than 600 mg of omega-3 LC-PUFAs in a 14 g serving.
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Terence A
authors.identifier: 0000-0003-2640-8189
authors.initials: TA
authors.lastname: Walsh
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Bevan
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Gachotte
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Cory M
authors.initials: CM
authors.lastname: Larsen
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: William A
authors.initials: WA
authors.lastname: Moskal
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: P A Owens
authors.initials: PA
authors.lastname: Merlo
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Lyudmila V
authors.initials: LV
authors.lastname: Sidorenko
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Ronnie E
authors.initials: RE
authors.lastname: Hampton
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Virginia
authors.initials: V
authors.lastname: Stoltz
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Dayakar
authors.initials: D
authors.lastname: Pareddy
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Geny I
authors.initials: GI
authors.lastname: Anthony
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Pudota B
authors.initials: PB
authors.lastname: Bhaskar
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Pradeep R
authors.initials: PR
authors.lastname: Marri
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Clark
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Adu-Peasah
authors.affiliation: Dow AgroSciences LLC, Research and Development, Indianapolis, Indiana, USA.
authors.forename: Steven T
authors.initials: ST
authors.lastname: Wensing
authors.affiliation: DSM Nutritional Products LLC, Columbia, Maryland, USA.
authors.forename: Ross
authors.initials: R
authors.lastname: Zirkle
authors.affiliation: DSM Nutritional Products LLC, Boulder, Colorado, USA.
authors.forename: James G
authors.initials: JG
authors.lastname: Metz
mesh_terms: D029688:Brassica napus / Q000235:genetics / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D024861:Genetic Enhancement / Q000379:methods*; D058086:Microalgae / Q000502:physiology*; D010938:Plant Oils / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods; D000074262:Rapeseed Oil
chemical_list: D010938:Plant Oils; D000074262:Rapeseed Oil; D004281:Docosahexaenoic Acids; D048630:Polyketide Synthases