title: Approaches to Determine Protein Ubiquitination Residue Types.
abstract: Ubiquitination is an important posttranslational modification in eukaryotic organisms and plays a central role in many signaling pathways in plants. Most ubiquitination typically occurs on substrate lysine residues, forming a covalent isopeptide bond. Some recent reports suggested ubiquitin can be attached to non-lysine sites such as serine/threonine, cysteine or the N-terminal methionine, via oxyester or thioester linkages, respectively. In the present protocol, we developed a convenient in vitro assay for investigating ubiquitination on Ser/Thr and Cys residues.
authors.affiliation: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No. 1 West Beichen Road, Beijing, 100101, P. R. China.|Graduate University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No. 1 West Beichen Road, Beijing, 100101, P. R. China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No. 1 West Beichen Road, Beijing, 100101, P. R. China. qxie@genetics.ac.cn.|Graduate University of Chinese Academy of Sciences, Beijing, 100049, P. R. China. qxie@genetics.ac.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xie
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D003545:Cysteine / Q000737:chemistry; D008715:Methionine / Q000737:chemistry; D008967:Molecular Biology / Q000379:methods*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012694:Serine / Q000737:chemistry; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D011506:Proteins; D025801:Ubiquitin; D012694:Serine; D008715:Methionine; D003545:Cysteine
keywords: Cysteine; Hydrolysis; Posttranslational modification; Serine/threonine; Ubiquitination