title: Synergistic effects of bioactive ions and micro/nano-topography on the attachment, proliferation and differentiation of murine osteoblasts (MC3T3).
abstract: Surface topography and chemical nature of biological materials play an important role in regulating cell behaviors. For the intention of improving the biological performance of Ti6Al4V, the hierarchical micro/nano-topographies containing bioactive ions (Ca(2+) and Mg(2+)) were fabricated in this study. Briefly, the hierarchical micro/nano-topography was constructed on Ti6Al4V surface via sandblasting, acid etching and alkali-hydrothermal treatment. Then Na(+) existing in the nano-topography was replaced by Ca(2+) and Mg(2+) through hydrothermal reaction. The surface topographies and chemical nature of native and treated samples were characterized using laser scanning microscope, X-ray Photoelectron Spectroscopy and field emission scanning electron microscopy with the energy-dispersive spectroscopy. Surface wettability was measured with a contact angle goniometer. A series of biological tests were carried out to evaluate the synergistic effects of bioactive ions and micro/nano-topography on the attachment, proliferation and differentiation of murine osteoblastic MC3T3 cells. The results of in vitro tests indicated that Ca(2+) and Mg(2+) in the titanium alloy surface had an affirmative effect on cells attachment, proliferation and differentiation. Cells grown onto micro/nano-structured surface with Ca(2+) implantation exhibited significantly higher differentiation levels of alkaline phosphatase activity and mineralization compared to that on micro/nano-structured surface with Mg(2+) implantation. This study provided a novel method to construct a favorable biological environment between tissues and implants.
authors.affiliation: Key Laboratory of High Efficiency and Clean Manufacturing, School of Mechanical Engineering, Shandong University, 17923, Jingshi Road, Jinan, 250061, Shandong, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Wang
authors.affiliation: Key Laboratory of High Efficiency and Clean Manufacturing, School of Mechanical Engineering, Shandong University, 17923, Jingshi Road, Jinan, 250061, Shandong, China. wanyi@sdu.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Key Laboratory of High Efficiency and Clean Manufacturing, School of Mechanical Engineering, Shandong University, 17923, Jingshi Road, Jinan, 250061, Shandong, China.
authors.forename: Zhanqiang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000737:chemistry; D000497:Alloys; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D007477:Ions / Q000737:chemistry; D008274:Magnesium / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D010006:Osteoblasts / Q000166:cytology*; D056951:Photoelectron Spectroscopy; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D017155:Wettability
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D007477:Ions; C031462:titanium alloy (TiAl6V4); D014025:Titanium; D000469:Alkaline Phosphatase; D008274:Magnesium; D002118:Calcium