title: Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment.
abstract: We investigated changes in PA levels by the treatment of human bone-marrow-derived mesenchymal stem cells (hBM-MSCs) in ischemic stroke in rat brain model and in cultured neuronal SH-SY5Y cells exposed to oxygen-glucose deprivation (OGD). In ischemic rat model, transient middle cerebral artery occlusion (MCAo) was performed for 2 h, followed by intravenous transplantation of hBM-MSCs or phosphate-buffered saline (PBS) the day following MCAo. Metabolic profiling analysis of PAs was examined in brains from three groups: control rats, PBS-treated MCAo rats (MCAo), and hBM-MSCs-treated MCAo rats (MCAo + hBM-MSCs). In ischemic cell model, SH-SY5Y cells were exposed to OGD for 24 h, treated with hBM-MSCs (OGD + hBM-MSCs) prior to continued aerobic incubation, and then samples were collected after coculture for 72 h. In the in vivo MCAo ischemic model, levels of some PAs in brain samples of the MCAo and MCAo + hBM-MSCs groups were significantly different from those of the control group. In particular, putrescine, cadaverine, and spermidine in brain tissues of the MCAo + hBM-MSCs group were significantly reduced in comparison to those in the MCAo group. In the in vitro OGD system, N (1)-acetylspermidine, spermidine, N (1)-acetylspermine, and spermine in cells of the OGD + hBM-MSCs group were significantly reduced compared to those of OGD group.
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Republic of Korea; Department of Physiology and Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 443-721, Republic of Korea.
authors.forename: Tae Hwan
authors.initials: TH
authors.lastname: Shin
authors.affiliation: Department of Physiology and Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 443-721, Republic of Korea.
authors.forename: Geetika
authors.initials: G
authors.lastname: Phukan
authors.affiliation: Department of Physiology and Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 443-721, Republic of Korea.
authors.forename: Jeom Soon
authors.initials: JS
authors.lastname: Shim
authors.affiliation: National Institute of Drug Quality Control, 48 Hai Ba Trung Street, Hanoi, Vietnam.
authors.forename: Duc-Toan
authors.initials: DT
authors.lastname: Nguyen
authors.affiliation: Pharmicell Co., Ltd., Seongnam 462-806, Republic of Korea.
authors.forename: Yongman
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Psychology, Central Michigan University, Health Professions Building, MI 48859, USA.
authors.forename: Justin D
authors.initials: JD
authors.lastname: Oh-Lee
authors.affiliation: Department of Physiology and Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 443-721, Republic of Korea.
authors.forename: Hyeon-Seong
authors.initials: HS
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Sunchon National University, Suncheon 540-950, Republic of Korea.
authors.forename: Man Jeong
authors.initials: MJ
authors.lastname: Paik
authors.affiliation: Department of Physiology and Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 443-721, Republic of Korea.
authors.forename: Gwang
authors.initials: G
authors.lastname: Lee