title: Complete Genome Analysis of an Enterovirus EV-B83 Isolated in China.
abstract: Enterovirus B83 (EV-B83) is a recently identified member of enterovirus species B. It is a rarely reported serotype and up to date, only the complete genome sequence of the prototype strain from the United States is available. In this study, we describe the complete genomic characterization of an EV-B83 strain 246/YN/CHN/08HC isolated from a healthy child living in border region of Yunnan Province, China in 2008. Compared with the prototype strain, it had 79.6% similarity in the complete genome and 78.9% similarity in the VP1 coding region, reflecting the great genetic divergence among them. VP1-coding region alignment revealed it had 77.2-91.3% with other EV-B83 sequences available in GenBank. Similarity plot analysis revealed it had higher identity with several other EV-B serotypes than the EV-B83 prototype strain in the P2 and P3 coding region, suggesting multiple recombination events might have occurred. The great genetic divergence with previously isolated strains and the extremely rare isolation suggest this serotype has circulated at a low epidemic strength for many years. This is the first report of complete genome of EV-B83 in China.
authors.affiliation: Yunnan Center for Disease Control and Prevention, Kunming, Yunnan Province, People's Republic of China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Tang
authors.affiliation: Yunnan Center for Disease Control and Prevention, Kunming, Yunnan Province, People's Republic of China.
authors.forename: Qiongfen
authors.initials: Q
authors.lastname: Li
authors.affiliation: Yunnan Center for Disease Control and Prevention, Kunming, Yunnan Province, People's Republic of China.
authors.forename: Bingjun
authors.initials: B
authors.lastname: Tian
authors.affiliation: Yunnan Center for Disease Control and Prevention, Kunming, Yunnan Province, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Yunnan Center for Disease Control and Prevention, Kunming, Yunnan Province, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Yunnan Center for Disease Control and Prevention, Kunming, Yunnan Province, People's Republic of China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Yunnan Center for Disease Control and Prevention, Kunming, Yunnan Province, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
mesh_terms: D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D002460:Cell Line; D002648:Child; D002681:China; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000821:virology; D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D058889:Molecular Typing; D012367:RNA, Viral / Q000235:genetics; D017423:Sequence Analysis, RNA
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; C000627674:VP1 protein, enterovirus B