title: Exploring an animal model of amodiaquine-induced liver injury in rats and mice.
abstract: Amodiaquine (AQ) is associated with a relatively high incidence of idiosyncratic drug-induced liver injury (IDILI) and agranulocytosis. A previous study reported that a combination of high dose AQ and glutathione (GSH) depletion led to liver injury. However, the characteristics of this toxicity were very different from AQ-induced liver injury in humans. We developed a model of AQ-induced liver injury with characteristics similar to the injury in humans by treating mice with lower doses of AQ for several weeks. In this study we found that not only did GSH depletion not increase AQ covalent binding to hepatic proteins at this lower dose, but also it paradoxically prevented the liver injury. We extended the model to rats and found AQ treatment led to a mild delayed onset liver injury that resolved despite continued treatment with AQ. Immunohistochemistry indicated the presence of Kupffer cell activation, apoptosis and hepatocyte proliferation in the liver. There was also an increase in serum IL-2, IL-5, IL-9, IL-12, MCP-1 and TGFβ, but a decrease in leptin. Coincident with the elevated serum ALT, the number of liver CD4(+) T-cells, IL-17 secreting cells and TH17/Treg cells increased at Week 3 and decreased during continued treatment. Increases in NK1.1+ cells and activated M2 macrophages were also observed during liver injury. These results suggest that the outcome of the liver injury was determined by the balance between effector and regulatory cells. Co-treatment with cyclosporin prevented AQ-induced liver injury, which supports an immune mechanism. Retinoic acid (RA), which has been reported to enhance natural killer (NK) cell activity, exacerbated AQ-induced liver injury. These results suggest that AQ-induced IDILI is immune mediated and the subsequent adaptation appears to represent immune tolerance.
authors.affiliation: a Faculty of Pharmacy , University of Toronto , Toronto , Ontario , Canada ;
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: a Faculty of Pharmacy , University of Toronto , Toronto , Ontario , Canada ;
authors.forename: Ping
authors.initials: P
authors.lastname: Cai
authors.affiliation: a Faculty of Pharmacy , University of Toronto , Toronto , Ontario , Canada ;
authors.forename: Imir
authors.initials: I
authors.lastname: Metushi
authors.affiliation: b Safety Assessment Toxicology , Genentech Inc. , South San Francisco , CA , USA ;
authors.forename: Jinze
authors.initials: J
authors.lastname: Li
authors.affiliation: a Faculty of Pharmacy , University of Toronto , Toronto , Ontario , Canada ;
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nakayawa
authors.affiliation: c Department of Toxicology , Centre for Research and Advanced Studies of the National Polytechnic Institute , Mexico , Mexico.
authors.forename: Libia
authors.initials: L
authors.lastname: Vega
authors.affiliation: a Faculty of Pharmacy , University of Toronto , Toronto , Ontario , Canada ;
authors.forename: Jack
authors.initials: J
authors.lastname: Uetrecht
mesh_terms: D000380:Agranulocytosis / Q000139:chemically induced / Q000276:immunology*; D000655:Amodiaquine / Q000633:toxicity; D000818:Animals; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000276:immunology*; D016572:Cyclosporine / Q000008:administration & dosage; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D011914:Rats, Inbred BN; D012075:Remission, Spontaneous; D013132:Spinocerebellar Degenerations; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D014212:Tretinoin / Q000008:administration & dosage
chemical_list: D016207:Cytokines; D000655:Amodiaquine; D014212:Tretinoin; D016572:Cyclosporine
keywords: Animal model; DILI; NK cells; TH17 cells; amodiaquine; covalent binding; hepatotoxicity; immune-mediated; macrophages; tolerance.