title: Identification, Isolation, and Functional Assay of Regulatory T Cells.
abstract: Two categories of regulatory T cells (Tregs), nTreg and iTreg, play vital roles in orchestrating the integrity of a host in the course of an immune response. Tregs commonly belong to CD4+ CD25+ T cells and they are characterized by a transcription factor - forkhead box P3 (FoxP3). Within the space of the last few years, interests have been drawn to Tregs as a therapeutic tool in several settings like autoimmune disease, transplantation, and tumor disorders. As a consequence, to assess the functional properties of Tregs, namely through their ability to suppress other cells, cytokine expression, and proliferation in a variety of conditions, it is mandatory to gain better approaches to this end. This would be beneficial in designing better-than-ever therapeutic methods with regard to Tregs properties. Gaining better insights into the underlying mechanisms of immune regulation, by means of straightforward and less time-consuming techniques, will hopefully permit the therapeutic application of these cells in the control of human disorders. This review aims at going through the basic methods for Treg isolation as well the efficiency of the commonly exerted in vitro assays of Tregs-mediated immune suppression.
authors.affiliation: a Department of Immunology, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Azimi
authors.affiliation: a Department of Immunology, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Aslani
authors.affiliation: b Department of Immunology, School of Medicine , Tabriz University of Medical Sciences , Tabriz, Iran.
authors.forename: Sahar
authors.initials: S
authors.lastname: Mortezagholi
authors.affiliation: c Department of Immunology, Faculty of Medical Sciences , Tarbiat Modares University , Tehran , Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Salek
authors.affiliation: d Department of Biochemistry and Immunology, Faculty of Medicine , Zabol University of Medical Sciences , Zabol , Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Javan
authors.affiliation: a Department of Immunology, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Rezaiemanesh
authors.affiliation: e Department of Immunology, School of Public Health , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Ghaedi
authors.affiliation: c Department of Immunology, Faculty of Medical Sciences , Tarbiat Modares University , Tehran , Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Gholamzad
authors.affiliation: a Department of Immunology, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Eisa
authors.initials: E
authors.lastname: Salehi
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D007108:Immune Tolerance; D007118:Immunoassay; D007167:Immunotherapy; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000502:physiology*
chemical_list: D015704:CD4 Antigens; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit
keywords: Cell isolation; functional assay; regulatory T cells; therapeutic tool