title: Influence of a low dose of silver nanoparticles on cerebral myelin and behavior of adult rats.
abstract: Nanoscale particles have large surface to volume ratio that significantly enhances their chemical and biological reactivity. Although general toxicity of nano silver (nanoAg) has been intensively studied in both in vitro and in vivo models, its neurotoxic effects are poorly known, especially those of low-dose exposure. In the present study we assess whether oral administration of nanoAg influences behavior of exposed rats and induces changes in cerebral myelin. We examine the effect of prolonged exposure of adult rats to small (10nm) citrate-stabilized nanoAg particles at a low dose of 0.2mg/kg b.w. (as opposed to the ionic silver) in a comprehensive behavioral analysis. Myelin ultrastructure and the expression of myelin-specific proteins are also investigated. The present study reveals slight differences with respect to behavioral effects of Ag(+)- but not nanoAg-treated rats. A weak depressive effect and hyperalgesia were observed after Ag(+) exposure whereas administration of nanoAg was found to specifically increase body weight and body temperature of animals. Both nanoAg and Ag(+) induce morphological disturbances in myelin sheaths and alter the expression of myelin-specific proteins CNP, MAG and MOG. These results suggest that the CNS may be a target of low-level toxicity of nanoAg.
authors.affiliation: Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawińskiego str., 02-106 Warsaw, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Dąbrowska-Bouta
authors.affiliation: Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, 4a Chodźki str., 20-093 Lublin, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Zięba
authors.affiliation: Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, 4a Chodźki str., 20-093 Lublin, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Orzelska-Górka
authors.affiliation: Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawińskiego str., 02-106 Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Skalska
authors.affiliation: Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawińskiego str., 02-106 Warsaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Sulkowski
authors.affiliation: Electron Microscopy Platform, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawińskiego str., 02-106 Warsaw, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Frontczak-Baniewicz
authors.affiliation: Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, 4a Chodźki str., 20-093 Lublin, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Talarek
authors.affiliation: Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, 4a Chodźki str., 20-093 Lublin, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Listos
authors.affiliation: Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawińskiego str., 02-106 Warsaw, Poland. Electronic address: lidkas@imdik.pan.pl.
authors.forename: Lidia
authors.initials: L
authors.lastname: Strużyńska
mesh_terms: D000818:Animals; D054022:Cerebrum / Q000187:drug effects*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D053768:Metal Nanoparticles / Q000009:adverse effects*; D009043:Motor Activity / Q000187:drug effects; D009186:Myelin Sheath / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D021641:Recognition, Psychology / Q000187:drug effects; D045442:Rotarod Performance Test; D018030:Silver Compounds / Q000009:adverse effects*
chemical_list: D018030:Silver Compounds
keywords: Behavioral tests; CNP; MAG; MOG; Nanosilver; Nanotoxicity; Neurotoxicity