title: Linkage Mapping of Stem Saccharification Digestibility in Rice.
abstract: Rice is the staple food of almost half of the world population, and in excess 90% of it is grown and consumed in Asia, but the disposal of rice straw poses a problem for farmers, who often burn it in the fields, causing health and environmental problems. However, with increased focus on the development of sustainable biofuel production, rice straw has been recognized as a potential feedstock for non-food derived biofuel production. Currently, the commercial realization of rice as a biofuel feedstock is constrained by the high cost of industrial saccharification processes needed to release sugar for fermentation. This study is focused on the alteration of lignin content, and cell wall chemotypes and structures, and their effects on the saccharification potential of rice lignocellulosic biomass. A recombinant inbred lines (RILs) population derived from a cross between the lowland rice variety IR1552 and the upland rice variety Azucena with 271 molecular markers for quantitative trait SNP (QTS) analyses was used. After association analysis of 271 markers for saccharification potential, 1 locus and 4 pairs of epistatic loci were found to contribute to the enzymatic digestibility phenotype, and an inverse relationship between reducing sugar and lignin content in these recombinant inbred lines was identified. As a result of QTS analyses, several cell-wall associated candidate genes are proposed that may be useful for marker-assisted breeding and may aid breeders to produce potential high saccharification rice varieties.
authors.affiliation: Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
authors.forename: Bohan
authors.initials: B
authors.lastname: Liu
authors.affiliation: Centre for Novel Agricultural Products, Department of Biology, University of York, York YO10 5DD, United Kingdom.
authors.forename: Leonardo D
authors.initials: LD
authors.lastname: Gómez
authors.affiliation: Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
authors.forename: Cangmei
authors.initials: C
authors.lastname: Hua
authors.affiliation: Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Sun
authors.affiliation: Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
authors.forename: Imran
authors.initials: I
authors.lastname: Ali
authors.affiliation: Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
authors.forename: Linli
authors.initials: L
authors.lastname: Huang
authors.affiliation: Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Yu
authors.affiliation: Centre for Novel Agricultural Products, Department of Biology, University of York, York YO10 5DD, United Kingdom.
authors.forename: Rachael
authors.initials: R
authors.lastname: Simister
authors.affiliation: Centre for Novel Agricultural Products, Department of Biology, University of York, York YO10 5DD, United Kingdom.
authors.forename: Clare
authors.initials: C
authors.lastname: Steele-King
authors.affiliation: Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Gan
authors.affiliation: Centre for Novel Agricultural Products, Department of Biology, University of York, York YO10 5DD, United Kingdom.
authors.forename: Simon J
authors.initials: SJ
authors.lastname: McQueen-Mason
mesh_terms: D056804:Biofuels; D002482:Cellulose / Q000378:metabolism*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D008031:Lignin / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci
chemical_list: D056804:Biofuels; D002482:Cellulose; D008031:Lignin