title: Protective effect of pilin protein with alum+naloxone adjuvant against acute pulmonary Pseudomonas aeruginosa infection.
abstract: Pseudomonas aeruginosa is an important opportunistic human pathogen that causes a wide variety of severe nosocomial infections. Type IV pili of P. aeruginosa are made up of polymerized pilin that aids in bacterial adhesion, biofilm formation and twitching motility. The aim of this study was to evaluate the efficacy of alum and naloxone (alum+NLX) as an adjuvant for P. aeruginosa recombinant PilA (r-PilA) as a vaccine candidate in the improvement of humoral and cellular immunity. Primary immunization with r-PilA in combination with alum+NLX followed by two booster shots was sufficient to generate robust cellular and humoral responses, which were Th1 and Th2 type responses consisting of IgG1 and IgG2a subtypes. Analysis of the cytokine response among immunized mice showed an increased production of IL-4, INF-γ and IL-17 by splenocytes upon stimulation by r-PilA. These sera were also able to reduce bacterial load in the lung tissue of challenged mice. The reduction of systemic bacterial spread resulted in increased survival rates in challenged immunized mice. In conclusion, immunization with r-PilA combined with alum+NLX evokes cellular and humoral immune responses, which play an important role in providing protection against acute P. aeruginosa lung infection among immunized mice.
authors.affiliation: Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Quality Control Department of Iran Gelatin Capsule Mfg. Co., Tehran, Iran.
authors.forename: Abbas Zare
authors.initials: AZ
authors.lastname: Banadkoki
authors.affiliation: Quality Control Department of Iran Gelatin Capsule Mfg. Co., Tehran, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Keshavarzmehr
authors.affiliation: Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Afshar
authors.affiliation: Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Aleyasin
authors.affiliation: Burn Research Center, Hazrat Fatima Hospital, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Fatemi
authors.affiliation: Burn Research Center, Hazrat Fatima Hospital, Iran University of Medical Sciences, Tehran, Iran; Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: bahador.behroz3@gmail.com.
authors.forename: Bahador
authors.initials: B
authors.lastname: Behrouz
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: hashemi@fbh.hiv.
authors.forename: Farhad B
authors.initials: FB
authors.lastname: Hashemi
mesh_terms: D000208:Acute Disease; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000534:Alum Compounds / Q000494:pharmacology*; D000818:Animals; D005260:Female; D036781:Fimbriae Proteins / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009270:Naloxone / Q000494:pharmacology*; D018410:Pneumonia, Bacterial / Q000276:immunology / Q000517:prevention & control; D011552:Pseudomonas Infections / Q000276:immunology / Q000517:prevention & control; D054406:Pseudomonas Vaccines / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D054406:Pseudomonas Vaccines; D036781:Fimbriae Proteins; C041524:aluminum sulfate; D009270:Naloxone
keywords: Adjuvant; Immunization; Pseudomonas aeruginosa; Pulmonary infection; r-PilA