title: Transcriptomic responses of marine medaka's ovary to hypoxia.
abstract: Hypoxia, an endocrine disruptor, is pressing global problem affecting marine organisms in over 400 "Dead Zones" worldwide. There is growing evident demonstrated the disruptive effect of hypoxia on reproductive systems of marine fish through the impairments of steroidogenic gene expression, leading to the alteration of sex hormone production in gonads. But the detailed molecular mechanism underlying the responses of female reproductive systems to hypoxic stress remains largely unknown. In the present report, we used marine medaka Oryzias melastigma as a model, together with high-throughput transcriptome sequencing and bioinformatics analysis, aiming to determine the changes in transcriptional signature in the ovary of marine fish under hypoxic stress. Our result discovered over two hundred differential expressed genes in ovary in response to hypoxia. The bioinformatics analysis together with quantitative RT-PCR validation on the deregulated genes highlighted the dysregulations of a number of female reproductive functions including interruptions of ovarian follicle development, gonad development and steroid metabolic process. Additionally, we revealed that these deregulations are through the modulation of leukemia inhibitory factor (LIF), insulin-like growth factor 1 receptor (IGF1R) and follicle stimulating hormone (FSH). The result of this work complements previous studies and provides additional insights into the underlying molecular mechanism of hypoxia-induced impairment of female reproductive system.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Hong Kong Special Administrative Region. Electronic address: kengplai@cityu.edu.hk.
authors.forename: Keng Po
authors.initials: KP
authors.lastname: Lai
authors.affiliation: School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region; State Key Laboratory of Agrobiotechnology, Chinese University of Hong Kong, Hong Kong Special Administrative Region. Electronic address: marcowanger@gmail.com.
authors.forename: Jing Woei
authors.initials: JW
authors.lastname: Li
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Hong Kong Special Administrative Region. Electronic address: annatse@hku.hk.
authors.forename: Anna Chung Kwan
authors.initials: AC
authors.lastname: Tse
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Hong Kong Special Administrative Region. Electronic address: angelahycheung@gmail.com.
authors.forename: Angela
authors.initials: A
authors.lastname: Cheung
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Hong Kong Special Administrative Region. Electronic address: Yuan.Wang2@ucsf.edu.
authors.forename: Simon
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region; State Key Laboratory of Agrobiotechnology, Chinese University of Hong Kong, Hong Kong Special Administrative Region. Electronic address: tf.chan@cuhk.edu.hk.
authors.forename: Ting Fung
authors.initials: TF
authors.lastname: Chan
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Hong Kong Special Administrative Region; State Key Laboratory in Marine Pollution, Hong Kong Special Administrative Region. Electronic address: bhrkong@cityu.edu.hk.
authors.forename: Richard Yuen Chong
authors.initials: RY
authors.lastname: Kong
authors.affiliation: State Key Laboratory in Marine Pollution, Hong Kong Special Administrative Region; Department of Science and Environmental Studies, Institute of Education, Hong Kong Special Administrative Region. Electronic address: rudolfwu@hkied.edu.hk.
authors.forename: Rudolf Shiu Sun
authors.initials: RS
authors.lastname: Wu
mesh_terms: D000818:Animals; D052244:Endocrine Disruptors / Q000633:toxicity; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000235:genetics / Q000378:metabolism; D006066:Gonads / Q000187:drug effects; D000860:Hypoxia; D053605:Leukemia Inhibitory Factor / Q000235:genetics / Q000378:metabolism; D009990:Oryzias / Q000254:growth & development / Q000378:metabolism*; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D017451:Receptors, Somatomedin / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D052244:Endocrine Disruptors; D029941:Fish Proteins; D053605:Leukemia Inhibitory Factor; D017451:Receptors, Somatomedin; D005640:Follicle Stimulating Hormone
keywords: Bioinformatics; Hypoxia; Ovary; Transcriptome