title: Cutaneous human papillomavirus genotypes in different kinds of skin lesions in Argentina.
abstract: Cutaneous human papillomaviruses (HPVs) comprise a large and highly heterogeneous virus group. Some of the cutaneous HPVs of the genus Beta have been suggested as a co-factor in the development of non-melanoma skin cancer (NMSC). The aim of this study was to determine cutaneous HPV prevalence and type-specific distribution in different kinds of skin lesions from Argentine patients visiting Dermatology Departments of three hospitals from Buenos Aires. A cross-sectional analysis was performed. HPV DNA was analyzed in (i) 3 patients with Epidermodysplasia verruciformis (EV) harboring benign lesions (BL) (n = 1) and squamous cell carcinoma (SCC) (n = 4); (ii) 240 non-EV patients harboring: (a) BL (n = 38), (b) Actinic Keratosis (AK) (n = 83), (c) SCC (n = 74), and (d) basal cell carcinoma (BCC) (n = 96). Detection and genotyping of 35 cutaneous HPV DNA was carried out by BGC-PCR and GP5+/6 + PCR followed by reverse line blot assay. In EV patients, Beta types were found in all lesions (5/5), including the potentially high-risk HPV types 5 and 8, mostly in multiple infections. In non-EV patients, cutaneous types were found in 50.0% of BL, 43.4% of AK, 31.1% of SCC, and 16.7% of BCC. Beta HPVs were the most frequently found in all lesions, being present in all AK and SCC cases that were positive for HPV. No type-specific correlation with lesion severity was found. In our series, a wide spectrum of cutaneous HPV types was detected in different skin lesions. A possible role for these HPVs in skin carcinogenesis deserves further study. J. Med. Virol. 89:352-357, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Virology, National Institute of Infectious Diseases-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: Rita Mariel
authors.initials: RM
authors.lastname: Correa
authors.affiliation: Department of Virology, National Institute of Infectious Diseases-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: Sara
authors.initials: S
authors.lastname: Vladimirsky
authors.affiliation: Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Daniëlle A M
authors.initials: DA
authors.lastname: Heideman
authors.affiliation: Hospital de Infecciosas "Dr. F. Muñiz", Buenos Aires, Argentina.
authors.forename: Mauro
authors.initials: M
authors.lastname: Coringrato
authors.affiliation: Hospital General de Agudos "Dr. Cosme Argerich", Buenos Aires, Argentina.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Abeldaño
authors.affiliation: Hospital de Infecciosas "Dr. F. Muñiz", Buenos Aires, Argentina.
authors.forename: Liliana
authors.initials: L
authors.lastname: Olivares
authors.affiliation: Instituto de Oncologia "Angel Roffo", Buenos Aires, Argentina.
authors.forename: Roxana
authors.initials: R
authors.lastname: Del Aguila
authors.affiliation: Department of Virology, National Institute of Infectious Diseases-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: Lidia Virginia
authors.initials: LV
authors.lastname: Alonio
authors.affiliation: Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Peter J F
authors.initials: PJ
authors.lastname: Snijders
authors.affiliation: Department of Virology, National Institute of Infectious Diseases-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: María Alejandra
authors.initials: MA
authors.lastname: Picconi
mesh_terms: D000368:Aged; D001118:Argentina / Q000453:epidemiology; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017193:Skin Diseases, Viral / Q000453:epidemiology* / Q000821:virology*
keywords: HPV genotyping; basal cell carcinoma; cutaneous human papillomavirus; skin lesions; squamous cell carcinoma