title: Chemical Characterization and Cytotoxic Activity of Blueberry Extracts (cv. Misty) Cultivated in Brazil.
abstract: Vaccinium corymbosum (L.) varieties cultivation is relatively recent in Brazil, but its production has been intensified given its good adaptability to the Southern Brazil climate. Blueberries are a rich source of phenolic compounds and contain significant levels of anthocyanins, flavonols, chlorogenic acids, and procyanidins, which lead to different biological activities. Chemical identification of skin and whole hydroalcoholic blueberry extracts (ExtSB and ExtWB) revealed the presence of anthocyanins concentrated in the skin and others chemicals compounds as quercetin glycosides, proanthocyanins dimers, citric, and chlorogenic acid in the pulp. Selectivity for tumor cell lines (Hep-2, HeLa, HT-29) using ExtSB and ExtWB extracts was observed through MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay after 24 h of treatment when compared to nontumor cells (MRC-5). Morphological changes and late stages of apoptotic and necrosis process were seen in HT-29 cell line after ExtWB treatment, compared to nontumor cell line MRC-5. These results are in agreement with other studies that indicate the activity of compounds such as anthocyanins and other molecules found in Southern Highbush blueberry variety, attributed to promote beneficial effects on health that may respond as cytotoxic natural agent and contribute to cancer treatment.
authors.affiliation: Laboratory of Genomics, Proteomics and DNA Repair, Biotechnology Inst, Univ. of Caxias do Sul, RS, Brazil.
authors.forename: Giovana
authors.initials: G
authors.lastname: Massarotto
authors.affiliation: Laboratory of Biotechnology of Natural and Synthetic Products, Biotechnology Inst, Univ. of Caxias do Sul, RS, Brazil.
authors.forename: Thiago
authors.initials: T
authors.lastname: Barcellos
authors.affiliation: Laboratory of Genomics, Proteomics and DNA Repair, Biotechnology Inst, Univ. of Caxias do Sul, RS, Brazil.
authors.forename: Charlene Silvestrin Celi
authors.initials: CS
authors.lastname: Garcia
authors.affiliation: Laboratory of Genomics, Proteomics and DNA Repair, Biotechnology Inst, Univ. of Caxias do Sul, RS, Brazil.
authors.forename: Ana Paula Carneiro
authors.initials: AP
authors.lastname: Brandalize
authors.affiliation: Laboratory of Biotechnology of Natural and Synthetic Products, Biotechnology Inst, Univ. of Caxias do Sul, RS, Brazil.
authors.forename: Sidnei
authors.initials: S
authors.lastname: Moura
authors.affiliation: Laboratory of Biotechnology of Natural and Synthetic Products, Biotechnology Inst, Univ. of Caxias do Sul, RS, Brazil.
authors.forename: Joséli
authors.initials: J
authors.lastname: Schwambach
authors.affiliation: Laboratory of Genomics, Proteomics and DNA Repair, Biotechnology Inst, Univ. of Caxias do Sul, RS, Brazil.
authors.forename: João Antonio Pêgas
authors.initials: JA
authors.lastname: Henriques
authors.affiliation: Laboratory of Genomics, Proteomics and DNA Repair, Biotechnology Inst, Univ. of Caxias do Sul, RS, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Roesch-Ely
mesh_terms: D000872:Anthocyanins / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis; D044946:Biflavonoids / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D029798:Blueberry Plants / Q000737:chemistry*; D001938:Brazil; D002392:Catechin / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D002726:Chlorogenic Acid / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D005638:Fruit / Q000737:chemistry*; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy*; D010636:Phenols / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D044945:Proanthocyanidins / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000872:Anthocyanins; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D044946:Biflavonoids; D010636:Phenols; D010936:Plant Extracts; D044945:Proanthocyanidins; D002726:Chlorogenic Acid; C017674:procyanidin; D002392:Catechin
keywords: Vaccinium corymbosum (L.); apoptosis; cancer; phenolics