title: Expression, Purification, and Enzymatic Analysis of Plant SUMO Proteases.
abstract: The conjugation of SUMO can profoundly change the behavior of substrate proteins, impacting a wide variety of cellular responses. SUMO proteases are emerging as key regulators of plant adaptation to its environment because of their instrumental role in the SUMO deconjugation process. Here, we describe how to express, purify, and determine SUMO deconjugation activity of a plant SUMO protease.
authors.affiliation: School of Biological and Biomedical Sciences, University of Durham, Durham, DH1 3LE, UK.
authors.forename: Gary
authors.initials: G
authors.lastname: Yates
authors.affiliation: School of Biological and Biomedical Sciences, University of Durham, Durham, DH1 3LE, UK.
authors.forename: Anjil
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: School of Biological and Biomedical Sciences, University of Durham, Durham, DH1 3LE, UK.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Orosa
authors.affiliation: School of Biological and Biomedical Sciences, University of Durham, Durham, DH1 3LE, UK. ari.sadanadom@durham.ac.uk.
authors.forename: Ari
authors.initials: A
authors.lastname: Sadanandom
mesh_terms: D018506:Gene Expression Regulation, Plant; D008967:Molecular Biology / Q000379:methods*; D010447:Peptide Hydrolases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D010944:Plants / Q000737:chemistry* / Q000235:genetics; D025841:Small Ubiquitin-Related Modifier Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D025841:Small Ubiquitin-Related Modifier Proteins; D010447:Peptide Hydrolases
keywords: AtOTS1; Deconjugation; Protease; Protein expression; Protein purification; SUMO