title: Designer Extracellular Matrix Based on DNA-Peptide Networks Generated by Polymerase Chain Reaction.
abstract: Cell proliferation and differentiation in multicellular organisms are partially regulated by signaling from the extracellular matrix. The ability to mimic an extracellular matrix would allow particular cell types to be specifically recognized, which is central to tissue engineering. We present a new functional DNA-based material with cell-adhesion properties. It is generated by using covalently branched DNA as primers in PCR. These primers were functionalized by click chemistry with the cyclic peptide c(RGDfK), a peptide that is known to predominantly bind to αvβ3 integrins, which are found on endothelial cells and fibroblasts, for example. As a covalent coating of surfaces, this DNA-based material shows cell-repellent properties in its unfunctionalized state and gains adhesiveness towards specific target cells when functionalized with c(RGDfK). These cells remain viable and can be released under mild conditions by DNase I treatment.
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457, Konstanz, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Finke
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457, Konstanz, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Bußkamp
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457, Konstanz, Germany.
authors.forename: Marilena
authors.initials: M
authors.lastname: Manea
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457, Konstanz, Germany. andreas.marx@uni-konstanz.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Marx
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D005109:Extracellular Matrix / Q000737:chemistry / Q000235:genetics*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D010455:Peptides; D004247:DNA
keywords: DNA; DNA nanotechnology; cell adhesion; integrins; polymerase chain reaction