title: An in-vitro evaluation of three types of drug-eluting microspheres loaded with irinotecan.
abstract: To compare the mechanical and chemical properties of three commercially available microspheres loaded with irinotecan. LifePearl (200 µm), DC Bead (100-300 µm), and Tandem (100 µm) microspheres were loaded with irinotecan. For loading, elution, and stability determinations, irinotecan concentrations were quantified using validated high-performance liquid chromatography methods. In-vitro elution was performed over 24 h using a USP 4 dissolution apparatus. Diameter measurements were performed using light microscopy. Time in suspension was considered as the time required for the microspheres to vacate 1/3 of the volume. All three microsphere types rapidly loaded irinotecan, with more than 95% loading at 1 h. In-vitro elution of irinotecan was rapid for LifePearl and DC Bead microspheres, with more than 98% elution at 1 h, and delayed for Tandem microspheres, with about 70% elution at 6 h. After loading with irinotecan, the average diameter of LifePearl and DC Bead microspheres was reduced by 9 and 18%, respectively, and was unchanged for Tandem microspheres. All three microsphere types lost 4-6% of the loaded irinotecan almost immediately upon placement in contrast: water and contrast: 5% dextrose, but further losses were minimal over 2 weeks. LifePearl microspheres remained longer in suspension (392±23 s) compared with DC Bead (154±13 s, P<0.001) and Tandem (198±19 s, P<0.001) microspheres. All three microsphere types load irinotecan rapidly. LifePearl and DC Bead microspheres elute irinotecan rapidly. Elution is delayed with Tandem microspheres. LifePearl microspheres show the longest time in suspension.
authors.affiliation: aClinic of Radiology, Minimally Invasive Therapies and Nuclear Medicine, SLK-Kliniken GmbH, Academic Hospital, Ruprecht-Karls-University Heidelberg bClinic of Diagnostic and Interventional Radiology, University Hospital Heidelberg, Heidelberg, Germany cMicroVention Inc., Tustin, California, USA.
authors.forename: Philippe L
authors.initials: PL
authors.lastname: Pereira
authors.forename: Stephen
authors.initials: S
authors.lastname: Plotkin
authors.forename: Renee
authors.initials: R
authors.lastname: Yu
authors.forename: Allison
authors.initials: A
authors.lastname: Sutter
authors.forename: Yue
authors.initials: Y
authors.lastname: Wu
authors.forename: Christof M
authors.initials: CM
authors.lastname: Sommer
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Cruise
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry*; D002166:Camptothecin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D004355:Drug Stability; D000077146:Irinotecan; D008863:Microspheres; D013535:Suspensions
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D013535:Suspensions; D000077146:Irinotecan; D002166:Camptothecin