title: Surface Functionalized Graphene Biosensor on Sapphire for Cancer Cell Detection.
abstract: Graphene has several unique physical, optical and electrical properties such as a two-dimensional (2D) planar structure, high optical transparency and high carrier mobility at room temperature. These make graphene interesting for electrical biosensing. Using a catalyst-free chemical vapor deposition (CVD) method, graphene film is grown on a sapphire substrate. There is a single or a few sheets as confirmed by Raman spectroscopy and atomic force microscopy (AFM). Electrical graphene biosensors are fabricated to detect large-sized biological analytes such as cancer cells. Human colorectal carcinoma cells are sensed by the resistance change of an active bio-functionalized graphene device as the cells are captured by the immobilized antibody surface. The functionalized sensors show an increase in resistance as large as ~20% of the baseline with a small number of adhered cells. This study suggests that the bio-functionalized electrical graphene sensors on sapphire, which is a highly transparent material, can potentially detect circulating tumor cells (CTCs) and monitor cellular electrical behavior while being compatible with fluorescence-based optical-detection bioassays.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Joe
authors.forename: Jeonghyun
authors.initials: J
authors.lastname: Hwang
authors.forename: Christelle
authors.initials: C
authors.lastname: Johnson
authors.forename: Ho-Young
authors.initials: HY
authors.lastname: Cha
authors.forename: Jo-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Xiling
authors.initials: X
authors.lastname: Shen
authors.forename: Michael G
authors.initials: MG
authors.lastname: Spencer
authors.forename: Sandip
authors.initials: S
authors.lastname: Tiwari
authors.forename: Moonkyung
authors.initials: M
authors.lastname: Kim
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D000912:Antibodies, Neoplasm / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D018625:Microscopy, Atomic Force; D009360:Neoplastic Cells, Circulating / Q000378:metabolism* / Q000473:pathology*
chemical_list: D000912:Antibodies, Neoplasm; D006108:Graphite; D000537:Aluminum Oxide