title: Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ.
abstract: Membrane fission is an essential cellular process by which continuous membranes split into separate parts. We have previously identified CtBP1-S/BARS (BARS) as a key component of a protein complex that is required for fission of several endomembranes, including basolateral post-Golgi transport carriers. Assembly of this complex occurs at the Golgi apparatus, where BARS binds to the phosphoinositide kinase PI4KIIIβ through a 14-3-3γ dimer, as well as to ARF and the PKD and PAK kinases. We now report that, when incorporated into this complex, BARS binds to and activates a trans-Golgi lysophosphatidic acid (LPA) acyltransferase type δ (LPAATδ) that converts LPA into phosphatidic acid (PA); and that this reaction is essential for fission of the carriers. LPA and PA have unique biophysical properties, and their interconversion might facilitate the fission process either directly or indirectly (via recruitment of proteins that bind to PA, including BARS itself).
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.|Telethon Institute of Genetics and Medicine (TIGEM), Via Campi Flegrei 34, Pozzuoli 80078, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pagliuso
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Carmen
authors.initials: C
authors.lastname: Valente
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Lucia Laura
authors.initials: LL
authors.lastname: Giordano
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Filograna
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Guiling
authors.initials: G
authors.lastname: Li
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Diego
authors.initials: D
authors.lastname: Circolo
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Turacchio
authors.affiliation: IRCCS SDN Istituto di Ricerca Diagnostica e Nucleare, Via Emanuele Gianturco 113, 80143 Naples, Italy.
authors.forename: Vincenzo Manuel
authors.initials: VM
authors.lastname: Marzullo
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Mandrich
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Zhukovsky
authors.affiliation: Italian Institute of Technology, Centre for Advanced Biomaterials for Health Care at CRIB, Largo Barsanti e Matteucci 53, Naples 80125, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Formiggini
authors.affiliation: Telethon Institute of Genetics and Medicine (TIGEM), Via Campi Flegrei 34, Pozzuoli 80078, Italy.
authors.forename: Roman S
authors.initials: RS
authors.lastname: Polishchuk
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Corda
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.|IRCCS SDN Istituto di Ricerca Diagnostica e Nucleare, Via Emanuele Gianturco 113, 80143 Naples, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Luini
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D000217:Acyltransferases / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D048749:Cytokinesis / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism*; D008246:Lysophospholipids / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D054462:p21-Activated Kinases / Q000378:metabolism
chemical_list: D048948:14-3-3 Proteins; D000217:Acyltransferases; D000429:Alcohol Oxidoreductases; D004268:DNA-Binding Proteins; D008246:Lysophospholipids; D010712:Phosphatidic Acids; D017853:Phosphotransferases (Alcohol Group Acceptor); D011493:Protein Kinase C; D054462:p21-Activated Kinases; C000610447:YWHAG protein, human; C096794:C-terminal binding protein; C018764:2-acylglycerophosphate acyltransferase; C581087:PI4KB protein, human; C089212:protein kinase D; C032881:lysophosphatidic acid