title: Bacterium-Expressed dsRNA Downregulates Microsporidia Nosema bombycis Gene Expression.
abstract: The microsporidia Nosema bombycis is the insect pathogen of pebrine disease severely destructive to sericulture production. Here, we describe the use of Escherichia coli HT115 strain (DE3) to express double-strand RNAs targeting the gene encoding ADP/ATP protein in N. bombycis. The results showed that dsRNAs deferentially suppressed the gene expression during N. bombycis infection in the silkworm, and the effect waned gradually. Our results, for the first time, provide a tool to utilize the dsRNA expressed by recombinant E. coli to control the pebrine disease of the domestic silkworm.
authors.affiliation: College of Life sciences, Chongqing Normal University, Chongqing, 401331, China.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Pan
authors.affiliation: College of Life sciences, Chongqing Normal University, Chongqing, 401331, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life sciences, Chongqing Normal University, Chongqing, 401331, China.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Dang
authors.affiliation: College of Life sciences, Chongqing Normal University, Chongqing, 401331, China.
authors.forename: Zhengang
authors.initials: Z
authors.lastname: Ma
authors.affiliation: College of Life sciences, Chongqing Normal University, Chongqing, 401331, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Sericultural and Apicultural Institute, Yunnan Academy of Agricultural Sciences, Mengzi, Yunnan, 661101, China.
authors.forename: Shiliang
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Life sciences, Chongqing Normal University, Chongqing, 401331, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.
authors.forename: Zeyang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: College of Life sciences, Chongqing Normal University, Chongqing, 401331, China.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Xu
mesh_terms: D000820:Animal Diseases / Q000382:microbiology / Q000517:prevention & control; D000818:Animals; D012831:Bombyx / Q000382:microbiology; D002352:Carrier Proteins / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D015536:Down-Regulation; D004926:Escherichia coli / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D016881:Microsporidiosis / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary; D016818:Nosema / Q000235:genetics* / Q000472:pathogenicity; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D011994:Recombinant Proteins; D013170:Spores
chemical_list: D002352:Carrier Proteins; D004271:DNA, Fungal; D005656:Fungal Proteins; D012330:RNA, Double-Stranded; D011994:Recombinant Proteins
keywords: ADP/ATP carrier protein; RNA interference; domestic silkworm