title: Synthesis and polymerase incorporation of β,γ-modified α-l-threofuranosyl thymine triphosphate mimics.
abstract: Three β,γ-modified α-l-threofuranosyl nucleoside triphosphates were synthesized. The β,γ-modified tTTPs undergo a single incorporation event with HIV RT but undergo multiple incorporations to form full-length product with engineered thermophilic polymerases.
authors.affiliation: Department of Chemistry and the Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Chemistry and the Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Kirsten N
authors.initials: KN
authors.lastname: Meek
authors.affiliation: Department of Chemistry and the Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Alexandra E
authors.initials: AE
authors.lastname: Rangel
authors.affiliation: Department of Chemistry and the Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA. Electronic address: heemstra@chem.utah.edu.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Heemstra
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism*; D054303:HIV Reverse Transcriptase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011122:Polyphosphates / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D018894:Reverse Transcriptase Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D013941:Thymine / Q000737:chemistry*
chemical_list: D011122:Polyphosphates; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase; D004259:DNA-Directed DNA Polymerase; C005692:triphosphoric acid; D013941:Thymine
keywords: Nucleosides; Polymerases; Primer extension; TNA; Triphosphate mimics