title: Synthesis and study on activity in vitro of the high purity human butyrylcholinesterase conjugated with gold nanoparticles.
abstract: The aim of this research was to design a method of immobilization of high-purity human butyrylcholinesterase on the surface of gold nanoparticles preserving the activity of the enzyme. In order to achieve this aim, the method of fractionation and purification of human butyrylcholinesterase from plasma was modified. The synthesis of 15-nm gold nanoparticles was carried out by citrated method. A method of conjugation of the high-purity butyrylcholinesterase with gold nanoparticles was developed. It was found that the Immobilization of butyrylcholinesterase on the surface of gold nanoparticles resulted in a significant (to 23%) increase in the specific activity of the enzyme.
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia. sokolov@ibppm.sgu.ru.
authors.forename: O I
authors.initials: OI
authors.lastname: Sokolov
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia.
authors.forename: N Y
authors.initials: NY
authors.lastname: Selivanov
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Bogatyrev
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia.
authors.forename: O G
authors.initials: OG
authors.lastname: Selivanova
authors.affiliation: Research Institute of Hygiene, Toxicology, and Occupational Pathology Federal Biomedical Agency, ul. Zemlyachki 12, Volgograd, 400048, Russia.
authors.forename: Y I
authors.initials: YI
authors.lastname: Velikorodnaya
authors.affiliation: Research Institute of Hygiene, Toxicology, and Occupational Pathology Federal Biomedical Agency, ul. Zemlyachki 12, Volgograd, 400048, Russia.
authors.forename: A Y
authors.initials: AY
authors.lastname: Pocheptsov
authors.affiliation: Research Institute of Hygiene, Toxicology, and Occupational Pathology Federal Biomedical Agency, ul. Zemlyachki 12, Volgograd, 400048, Russia.
authors.forename: B N
authors.initials: BN
authors.lastname: Filatov
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia.
authors.forename: S Y
authors.initials: SY
authors.lastname: Shchyogolev
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Dykman
mesh_terms: D002091:Butyrylcholinesterase / Q000737:chemistry / Q000302:isolation & purification; D004795:Enzyme Stability; D017612:Gold Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry; D010316:Particle Size
chemical_list: D017612:Gold Compounds; D002091:Butyrylcholinesterase