title: Effective Antisense Gene Regulation via Noncationic, Polyethylene Glycol Brushes.
abstract: Negatively charged nucleic acids are often complexed with polycationic transfection agents before delivery. Herein, we demonstrate that a noncationic, biocompatible polymer, polyethylene glycol, can be used as a transfection vector by forming a brush polymer-DNA conjugate. The brush architecture provides embedded DNA strands with enhanced nuclease stability and improved cell uptake. Because of the biologically benign nature of the polymer component, no cytotoxicity was observed. This approach has the potential to address several long-lasting challenges in oligonucleotide therapeutics.
authors.affiliation: Department of Chemistry and Chemical Biology, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: Xueguang
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Chemistry and Chemical Biology, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: Fei
authors.initials: F
authors.lastname: Jia
authors.affiliation: Department of Chemistry and Chemical Biology, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: Xuyu
authors.initials: X
authors.lastname: Tan
authors.affiliation: Department of Chemistry and Chemical Biology, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: Dali
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Chemical Biology, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Chemistry and Chemical Biology, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: Jiamin
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Chemistry and Chemical Biology, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D016373:DNA, Antisense / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011092:Polyethylene Glycols / Q000737:chemistry*; D014162:Transfection
chemical_list: D016373:DNA, Antisense; D011092:Polyethylene Glycols