title: Identification of Four Kinds of 2',3'-cNMPs in Escherichia coli and a Method for Their Preparation.
abstract: Four kinds of 2',3'-cNMPs have been identified in animals and plants, and their physiological roles are also suggested. However, in prokaryotes, while 2',3'-cCMP and 2',3'-cUMP are reported, 2',3'-cGMP or 2',3'-cAMP have never been identified from bacteria or archaea. In addition, there has been no biological method to prepare these cyclic nucleotides. During the study of a novel gene LfliZ from deep-sea bacterium Pseudoalteromonas sp. SM9913, we found that the recombinant LfliZ oligomers contained endogenous substrates, which were then identified to be 2',3'-cCMP, 2',3'-cUMP, 2',3'-cGMP, and 2',3'-cAMP, showing the first evidence for the biological existence of four kinds of 2',3'-cNMPs in prokaryotes. In addition, we further developed a biological method to simultaneously prepare four kinds of 2',3'-cNMPs from Escherichia coli through expressing LfliZ.
authors.forename: Ang
authors.initials: A
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Qi
authors.initials: Q
authors.lastname: Sheng
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Zheng
authors.forename: Jin-Yu
authors.initials: JY
authors.lastname: Yang
authors.forename: Ping-Yi
authors.initials: PY
authors.lastname: Li
authors.forename: Mei
authors.initials: M
authors.lastname: Shi
authors.forename: Bai-Cheng
authors.initials: BC
authors.lastname: Zhou
authors.affiliation: Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology , Qingdao, China.
authors.forename: Yu-Zhong
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Xiu-Lan
authors.initials: XL
authors.lastname: Chen
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D013058:Mass Spectrometry; D009712:Nucleotides, Cyclic / Q000737:chemistry* / Q000145:classification; D010944:Plants / Q000737:chemistry; D031161:Pseudoalteromonas / Q000737:chemistry / Q000235:genetics; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009712:Nucleotides, Cyclic