title: Effect of Fagonia arabica on thrombin induced release of t-PA and complex of PAI-1 tPA in cultured HUVE cells.
abstract: Fagonia arabica (FA) possesses a thrombolytic property which has been earlier reported in our laboratory. Current study was undertaken to investigate the effect of aqueous extract of FA on thrombin-induced tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) release from cultured human umbilical vein endothelial cell line (HUVE) for studying its clot lytic activity. For this, establishment of cell line model has been done by isolating the cells from human umbilical cord. Cell toxicity was evaluated using XTT assay. Estimation of t-PA and PAI-1 t-PA complex were done using ELISA technique. Thrombin treatment induces the t-PA and PAI-1 release from HUVE cell line, and FA treatment was found to antagonize the thrombin induced t-PA and PAI-1 release. Our preliminary results suggest that FA may be used as an alternative to thrombolytic drug. However, study demands further experiments using animal model of thrombosis to establish the role of FA as a novel thrombolytic drug.
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India.
authors.forename: Prutha D
authors.initials: PD
authors.lastname: Aloni
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India.
authors.forename: Amit R
authors.initials: AR
authors.lastname: Nayak
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India.
authors.forename: Sweta R
authors.initials: SR
authors.lastname: Chaurasia
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India.
authors.forename: Jayant Y
authors.initials: JY
authors.lastname: Deopujari
authors.affiliation: Matru Sewa Sangh, Maternity Home, Kothi Road, Mahal, Nagpur 440002, Maharashtra, India.
authors.forename: Chhaya
authors.initials: C
authors.lastname: Chourasia
authors.affiliation: Environmental Genomics Unit, NEERI, Nehru Marg, Nagpur 440020, Maharashtra, India.
authors.forename: Hemant J
authors.initials: HJ
authors.lastname: Purohit
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India.
authors.forename: Girdhar M
authors.initials: GM
authors.lastname: Taori
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India.
authors.forename: Hatim F
authors.initials: HF
authors.lastname: Daginawala
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India.
authors.forename: Rajpal S
authors.initials: RS
authors.lastname: Kashyap
keywords: Fagonia arabica (FA); HUVE cell line; Plasminogen activator inhibitor-1 (PAI-1); Thrombin; Tissue plasminogen activator (t-PA)