title: Transplantation of a bacterial consortium ameliorates trinitrobenzenesulfonic acid-induced colitis and intestinal dysbiosis in rats.
abstract: To investigate the effects of a defined bacterial consortium on trinitrobenzenesulfonic acid (TNBS)-induced colitis and intestinal dysbiosis in rats.
  
  
  
  Rats with TNBS-induced colitis were treated with ceftriaxone and/or a mixture of ten bacterial strains isolated from mouse feces for continuous 24 days. Macroscopic and histopathological parameters in colonic tissue were compared, as were myeloperoxidase enzyme activity and cytokine levels. Patterns of intestinal microbiota were assessed by PCR-denaturing gradient gel electrophoresis, the abundance of selected microbial groups was evaluated by qPCR.
  
  
  
  Transplantation of the bacterial consortium showed anti-inflammatory activity in the intestines of rats with TNBS-induced colitis and contributed to the rapid re-establishment of intestinal microbial equilibrium. A defined bacterial consortium may be a viable therapeutic option for the treatment inflammatory bowel disease.
authors.affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wen
authors.affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University, China.
authors.forename: Yinhui
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tang
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000382:microbiology / Q000628:therapy*; D003106:Colon / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D064806:Dysbiosis / Q000139:chemically induced / Q000382:microbiology / Q000628:therapy*; D000069467:Fecal Microbiota Transplantation; D005243:Feces / Q000382:microbiology*; D000069196:Gastrointestinal Microbiome; D015212:Inflammatory Bowel Diseases / Q000628:therapy; D007422:Intestines / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D051379:Mice; D059013:Microbial Consortia; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D014302:Trinitrobenzenesulfonic Acid / Q000008:administration & dosage*
chemical_list: D016207:Cytokines; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase
keywords: antibiotics; bacterial consortium transplantation; inflammatory bowel disease; intestinal microbiota