title: A calcium-dependent acyltransferase that produces N-acyl phosphatidylethanolamines.
abstract: More than 30 years ago, a calcium-dependent enzyme activity was described that generates N-acyl phosphatidylethanolamines (NAPEs), which are precursors for N-acyl ethanolamine (NAE) lipid transmitters, including the endocannabinoid anandamide. The identity of this calcium-dependent N-acyltransferase (Ca-NAT) has remained mysterious. Here, we use activity-based protein profiling to identify the poorly characterized serine hydrolase PLA2G4E as a mouse brain Ca-NAT and show that this enzyme generates NAPEs and NAEs in mammalian cells.
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.|The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ogura
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.|The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: William H
authors.initials: WH
authors.lastname: Parsons
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.|The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Siddhesh S
authors.initials: SS
authors.lastname: Kamat
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.|The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Cravatt
mesh_terms: D000217:Acyltransferases / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D010714:Phosphatidylethanolamines / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D010714:Phosphatidylethanolamines; D000217:Acyltransferases; D002118:Calcium