title: N(6)-Methyladenosine RNA Modification Regulates Shoot Stem Cell Fate in Arabidopsis.
abstract: N(6)-Methyladenosine (m(6)A) represents the most prevalent internal modification on mRNA and requires a multicomponent m(6)A methyltransferase complex in mammals. How their plant counterparts determine the global m(6)A modification landscape and its molecular link to plant development remain unknown. Here we show that FKBP12 INTERACTING PROTEIN 37 KD (FIP37) is a core component of the m(6)A methyltransferase complex, which underlies control of shoot stem cell fate in Arabidopsis. The mutants lacking FIP37 exhibit massive overproliferation of shoot meristems and a transcriptome-wide loss of m(6)A RNA modifications. We further demonstrate that FIP37 mediates m(6)A RNA modification on key shoot meristem genes inversely correlated with their mRNA stability, thus confining their transcript levels to prevent shoot meristem overproliferation. Our results suggest an indispensable role of FIP37 in mediating m(6)A mRNA modification, which is required for maintaining the shoot meristem as a renewable source for continuously producing all aerial organs in plants.
authors.affiliation: Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, 10 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Lisha
authors.initials: L
authors.lastname: Shen
authors.affiliation: Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, 10 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Gu
authors.affiliation: Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, 10 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, 10 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Zhi Wei Norman
authors.initials: ZW
authors.lastname: Teo
authors.affiliation: South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors.forename: Xingliang
authors.initials: X
authors.lastname: Hou
authors.affiliation: Singapore-MIT Alliance for Research and Technology, Campus for Research Excellence and Technical Enterprise (CREATE), Singapore 138602, Singapore.
authors.forename: Weiling Maggie
authors.initials: WM
authors.lastname: Cai
authors.affiliation: Singapore-MIT Alliance for Research and Technology, Campus for Research Excellence and Technical Enterprise (CREATE), Singapore 138602, Singapore.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Dedon
authors.affiliation: Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, 10 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, 10 Science Drive 4, Singapore 117543, Singapore. Electronic address: dbsyuhao@nus.edu.sg.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018519:Meristem / Q000166:cytology* / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D018520:Plant Shoots / Q000166:cytology* / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D018749:RNA, Plant / Q000737:chemistry* / Q000235:genetics; D016601:RNA-Binding Proteins; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; C488384:FIP37 protein, Arabidopsis; D018749:RNA, Plant; D016601:RNA-Binding Proteins; C010223:N-methyladenosine; D000241:Adenosine