title: Laccase multigene families in Agaricomycetes.
abstract: Here we present the results of the exploration of laccase multigene families (MGFs) in basidiomycetous fungi from different taxonomic groups using a next generation sequencing (NGS) technology. In our study, multiple laccase genes were identified in all of the investigated fungi (13 species) from Polyporaceae, Phanerochaetaceae, Meruliaceae, Pleurotaceae, Physalacriaceae, and Peniophoraceae families. It was shown that phylogenetic positioning of the newly identified sequences exhibit patterns of clusterization with respect to enzyme properties. This can be a potentially useful tool for selecting naturally existing laccases with different physicochemical characteristics relevant to different biotechnological applications. Moreover, the method developed in this study can be used in the screening of environmental samples and fast characterization of laccase MGFs in newly identified fungal species.
authors.affiliation: Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Moiseenko
authors.affiliation: N.I. Vavilov Institute of General Genetics Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Liliya G
authors.initials: LG
authors.lastname: Maloshenok
authors.affiliation: Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Daria V
authors.initials: DV
authors.lastname: Vasina
authors.affiliation: N.I. Vavilov Institute of General Genetics Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Bruskin
authors.affiliation: Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Tyazhelova
authors.affiliation: Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Koroleva
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D019143:Evolution, Molecular; D005800:Genes, Fungal; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D042845:Laccase / Q000235:genetics*; D005810:Multigene Family; D010802:Phylogeny
chemical_list: D042845:Laccase
keywords: Basidiomycetes; Laccase; Multigene family; Next generation sequencing; Phylogeny