title: Selective cholesterol adsorption by molecular imprinted polymeric nanospheres and application to GIMS.
abstract: Molecular imprinted polymers (MIPs) are tailor-made materials with selective recognition to the target. The goals of this study were to prepare cholesterol imprinted polymeric nanospheres (CIPNs) and optimize their adsorption parameters and also to use CIPNs for adsorption of cholesterol (CHO), which is an important physiological biomacromolecule, from gastrointestinal mimicking solution (GIMS). Pre-polymerization complex was prepared using CHO as template and N-methacryloylamido-(l)-phenylalanine methyl ester (MAPA). This complex was polymerized with 2-hydroxyethyl methacrylate (HEMA). CHO was removed by MeOH and tetrahydrofuran (THF). Adsorption studies were performed after chacterization studies to interrogate the effects of time, initial concentration, temperature, and ionic strength on CHO adsorption onto CIPNs. Maximum adsorption capacity (714.17mg/g) was higher than that of cholesterol imprinted polymers in literature. Pseudo-second-order kinetics and Langmuir isotherm fitted best with the adsorption onto CIPNs. 86% of adsorbed cholesterol was desorbed with MeOH:HAc (80:20, v/v) and CIPNs were used in adsorption-desorption cycle for 5-times with a decrease as 12.28%. CHO analogues; estron, estradiol, testosterone, and progesterone were used for competitive adsorption. The relative selectivity coefficients of CINPs for cholesterol/estron and cholesterol/testosterone were 3.84 and 10.47 times greater than the one of non-imprinted polymeric nanospheres (NIPNs) in methanol, respectively.
authors.affiliation: Aksaray University, Technical Vocational School of Higher Education, Department of Chemistry and Chemical Processing Technology, Aksaray, Turkey; Dokuz Eylul University, The Graduate School of Natural and Applied Sciences, Izmir, Turkey. Electronic address: tkalburcu@gmail.com.
authors.forename: Tülden
authors.initials: T
authors.lastname: Inanan
authors.affiliation: Dokuz Eylul University, Faculty of Science, Department of Chemistry, Izmir, Turkey.
authors.forename: Nalan
authors.initials: N
authors.lastname: Tüzmen
authors.affiliation: Ege University, Faculty of Science, Department of Biochemistry, Izmir, Turkey.
authors.forename: Sinan
authors.initials: S
authors.lastname: Akgöl
authors.affiliation: Hacettepe University, Faculty of Science, Department of Chemistry, Ankara, Turkey.
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D001826:Body Fluids / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D007700:Kinetics; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D009994:Osmolar Concentration; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors
chemical_list: D008689:Methacrylates; D011108:Polymers; C005044:hydroxyethyl methacrylate; D002784:Cholesterol
keywords: Biomacromolecule; Cholesterol; Gastrointestinal mimicking solution; Imprinting; Selectivity