title: Quantification of Polybrominated and Polychlorinated Biphenyls in Human Matrices by Isotope-Dilution Gas Chromatography-Tandem Mass Spectrometry.
abstract: We have developed a highly sensitive and selective analytical method capable of quantifying a total of 15 polybrominated and polychlorinated biphenyls (11 PBBs and 4 PCBs) in human serum. Samples were subjected to liquid-liquid extraction followed by solid-phase extraction prior to measurement using gas chromatography-tandem mass spectrometry in multiple reaction monitoring mode. Quantification was performed using isotope-dilution calibration covering a concentration range of 0.005-12.5 ng/mL. Limits of detection for all target compounds were in the low range (0.7-6.5 pg/mL). The method was validated using in-house pooled human serum fortified at two concentrations (0.5 ng/mL and 1.0 ng/mL), whole semen fortified at one concentration (0.25 ng/mL), and NIST Standard Reference Material (SRM) 1958, which includes five of the target compounds. Method accuracies for all target compounds ranged from 84 to 119% with relative standard deviations (RSDs) of <19%. The measured values for the five target compounds present in the SRM agreed with the certified reference values (89-119% accuracy with RSDs <9%). As this method was developed to support ongoing epidemiologic investigations, we evaluated its suitability by analyzing subsets of serum and whole semen samples from the Michigan PBB Registry cohort. PBB-153, PCB-118, PCB-138, PCB-153 and PCB-180 were detected in all serum samples analyzed, with PBB-77 and PBB-101 detected less frequently in serum. PBB-153, PCB-118, PCB-138, PCB-153 and PCB-180 were detected in at least one whole semen sample.
authors.affiliation: Department of Environmental Health, Rollins School of Public Health, Emory University, 1518 Clifton Road, Atlanta, GA 30322, USA.
authors.forename: M Elizabeth
authors.initials: ME
authors.lastname: Marder
authors.affiliation: Department of Environmental Health, Rollins School of Public Health, Emory University, 1518 Clifton Road, Atlanta, GA 30322, USA.
authors.forename: Parinya
authors.initials: P
authors.lastname: Panuwet
authors.affiliation: Department of Environmental Health, Rollins School of Public Health, Emory University, 1518 Clifton Road, Atlanta, GA 30322, USA.
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Hunter
authors.affiliation: Department of Environmental Health, Rollins School of Public Health, Emory University, 1518 Clifton Road, Atlanta, GA 30322, USA.
authors.forename: P Barry
authors.initials: PB
authors.lastname: Ryan
authors.affiliation: Department of Epidemiology, Rollins School of Public Health, Emory University, 1518 Clifton Road, Atlanta, GA 30322, USA.
authors.forename: Michele
authors.initials: M
authors.lastname: Marcus
authors.affiliation: Department of Environmental Health, Rollins School of Public Health, Emory University, 1518 Clifton Road, Atlanta, GA 30322, USA dbbarr@emory.edu.
authors.forename: Dana Boyd
authors.initials: DB
authors.lastname: Barr
mesh_terms: D004781:Environmental Exposure / Q000032:analysis*; D004785:Environmental Pollutants / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D011075:Polybrominated Biphenyls / Q000097:blood*; D011078:Polychlorinated Biphenyls / Q000097:blood*; D053719:Tandem Mass Spectrometry
chemical_list: D004785:Environmental Pollutants; D011075:Polybrominated Biphenyls; C070055:2,3',4,4',5-pentachlorobiphenyl; C410127:PCB 180; C029790:2,2',3',4,4',5-hexachlorobiphenyl; C018566:2,4,5,2',4',5'-hexabromobiphenyl; D011078:Polychlorinated Biphenyls; C014024:2,4,5,2',4',5'-hexachlorobiphenyl