title: Evaluation of platelet function using multiple electrode platelet aggregometry in dogs with septic peritonitis.
abstract: OBJECTIVE
  To assess platelet function via multiple electrode platelet aggregometry (MEPA) in dogs with septic peritonitis and in healthy dogs. The secondary aim was to determine if there is prognostic significance to changes in platelet function observed in septic dogs.
  
  
  METHODS
  Prospective, observational cohort study conducted from January 2012 to March 2014.
  
  
  METHODS
  University teaching hospital.
  
  
  METHODS
  Twenty dogs with septic peritonitis and 23 healthy dogs.
  
  
  METHODS
  None.
  
  
  RESULTS
  MEPA using arachidonic acid, adenosine diphosphate, and collagen (COL) as agonists was measured within 24 hours of diagnosis of sepsis. Compared to healthy dogs, platelet aggregation was reduced in dogs with septic peritonitis for all agonists (P < 0.01). Overall mortality rate was 40%. MEPA in response to COL was significantly reduced in nonsurvivors compared to survivors (P = 0.019). Using receiver-operating characteristic curve statistics, a COL-activated MEPA less than 43.5 aggregation units had a sensitivity and specificity of 85.7% and 90.9%, respectively, for predicting nonsurvival in dogs with septic peritonitis.
  
  
  CONCLUSIONS
  Circulating platelets from dogs with septic peritonitis have diminished aggregation in response to multiple platelet agonists. MEPA may serve as an assessment tool for illness severity in this patient population.
authors.affiliation: Section of Emergency and Critical Care, Department of Clinical Science and Services, Royal Veterinary College, University of London, London, UK. rhli@ucdavis.edu.|Dr. Li's current address: Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, UC Davis, Davis, CA. rhli@ucdavis.edu.
authors.forename: Ronald H L
authors.initials: RH
authors.lastname: Li
authors.affiliation: Section of Emergency and Critical Care, Department of Clinical Science and Services, Royal Veterinary College, University of London, London, UK.
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Chan
mesh_terms: D000818:Animals; D015331:Cohort Studies; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs; D004566:Electrodes; D005260:Female; D008297:Male; D010538:Peritonitis / Q000097:blood / Q000662:veterinary*; D010979:Platelet Function Tests / Q000662:veterinary*; D011446:Prospective Studies; D012372:ROC Curve; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000097:blood / Q000662:veterinary*
keywords: canine; hemostasis; multiple organ dysfunction syndrome; platelet dysfunction; sepsis