title: Combined effects of transferrin and thyroid hormone during oligodendrogenesis In vitro.
abstract: Thyroid hormones (THs) and transferrin (Tf) are factors capable of favoring myelination due to their positive effects on oligodendroglial cell (OLG) differentiation. The first notion of a combined effect of apotransferrin (aTf) and TH emerged from experiments conducted in young hyperthyroid animals, which showed a seven-fold increase in the expression of Tf mRNA and precocious myelination when compared with control animals. The mechanism underlying this phenomenon in young hyperthyroid rats could consist of an increase in Tf synthesis, which in the CNS is almost exclusively produced by OLG. Overall, our results show that, during the initial stages of OLG differentiation, Tf synthesis triggers thyroid hormone receptor alpha 1 (TRα1) expression in the subventricular zone (SVZ) and promotes proliferating cells to become responsive to this trophic factor. Exposure to TH could then regulate Tf expression through TRα1 and promote the induction of thyroid hormone receptor beta (TRβ) expression, which mediates TH effects on myelination through the activation of final OLG differentiation. This regulation of the combined effects of Tf and THs implies that both factors are fundamental actors during oligodendrogenesis. GLIA 2016;64:1879-1891.
authors.affiliation: Department of Biological Chemistry, Biological and Physical Chemistry Institute (IQUIFIB-CONICET), School of Pharmacy and Biochemistry, Universidad De Buenos Aires, Buenos Aires, Argentina.
authors.forename: L N
authors.initials: LN
authors.lastname: Marziali
authors.affiliation: Department of Neurology, Institute for Neurological Research Raúl Carrea, FLENI, Buenos Aires, Argentina.
authors.forename: J
authors.initials: J
authors.lastname: Correale
authors.affiliation: Laboratory of Regenerative and Protective Therapies of the Central Nervous System, Fundación Instituto Leloir-IIBBA CONICET, Buenos Aires, Argentina.
authors.forename: C I
authors.initials: CI
authors.lastname: Garcia
authors.affiliation: Department of Biological Chemistry, Biological and Physical Chemistry Institute (IQUIFIB-CONICET), School of Pharmacy and Biochemistry, Universidad De Buenos Aires, Buenos Aires, Argentina. jpasquin@qb.ffyb.uba.ar.
authors.forename: J M
authors.initials: JM
authors.lastname: Pasquini
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D020547:Lateral Ventricles / Q000166:cytology; D004676:Myelin Basic Protein / Q000378:metabolism; D009836:Oligodendroglia / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics / Q000378:metabolism; D011988:Receptors, Thyroid Hormone / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000187:drug effects; D013963:Thyroid Hormones; D014168:Transferrin / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D051741:Kruppel-Like Transcription Factors; D004676:Myelin Basic Protein; D011988:Receptors, Thyroid Hormone; D013963:Thyroid Hormones; D014168:Transferrin; D049452:Green Fluorescent Proteins; C077017:Klf9 protein, rat; D020796:Receptor, Platelet-Derived Growth Factor alpha
keywords: myelin; oligodendrocytes; thyroid hormone receptors; thyroid hormones; transferrin