title: Multiple small versus few large amount aspirations for bone marrow harvesting in autologous and allogeneic bone marrow transplantation.
abstract: For successful bone marrow transplantation it is necessary to obtain enough progenitor cells during the bone marrow (BM) harvesting procedure. Most centers are using multiple aspirations of maximum 2 ml BM (A), while other centers are using few larger amount aspirations for BM harvesting (B). There is still a discussion about possible differences in graft composition between A and B. To evaluate the feasibility in children we evaluated twenty BM harvestings that were performed in 18 donors, 7 autologous (median age 6.93y; 2.48-16.6) and 13 allogeneic donors (median age 19.75y; 6.45-50.7). A and B were performed crosswise by 2 operators starting with A (2 ml) or B (100 ml) changing to B or A, collecting identically amounts with both methods. We found no statistically significant difference between A and B for MNC, T-cells, and CFU (MNC/ml 824572 versus 725000, p = 0.728; MNC/kg 3.1 107versus 2.9 107, p = 0.296; CD3/ml 162500 versus 300000, p = 0.310; CFU/105MNC 1678 versus 1315, p = 0.094), but for CD34+ cells (CD34/kg 2.62 versus 2.09, p = 0.045). BM harvest by the large amount few punctures method (B) is as sufficient as the commonly used small amount frequent punctures method (A), and could be therefore used equally.
authors.affiliation: Department of Pediatrics, St. Anna Kinderspital, Medical University Vienna, Austria. Electronic address: volker.witt@stanna.at.
authors.forename: Volker
authors.initials: V
authors.lastname: Witt
authors.affiliation: Department of Pediatrics, St. Anna Kinderspital, Medical University Vienna, Austria.
authors.forename: Herbert
authors.initials: H
authors.lastname: Pichler
authors.affiliation: Department of Pediatrics, St. Anna Kinderkrebsforschung, CCRI, Medical University Vienna, Austria.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Fritsch
authors.affiliation: Department of Pediatrics, St. Anna Kinderspital, Medical University Vienna, Austria.
authors.forename: Christina
authors.initials: C
authors.lastname: Peters
mesh_terms: D000293:Adolescent; D000328:Adult; D064591:Allografts; D064592:Autografts; D001853:Bone Marrow; D016026:Bone Marrow Transplantation / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019152:Paracentesis / Q000379:methods*; D014019:Tissue Donors
keywords: Bone marrow; HPC-BM; Harvesting; Stem cell transplantation