title: Farnesol production in Escherichia coli through the construction of a farnesol biosynthesis pathway - application of PgpB and YbjG phosphatases.
abstract: Farnesol is a sesquiterpenoid alcohol that has important industrial and medical potential. It is usually synthesized from farnesyl diphosphate (FPP) by farnesol synthase in plants. FPP accumulation can cause up-regulation of phosphatases capable of FPP hydrolysis, resulting in farnesol production in Escherichia coli. We found that PgpB and YbjG, two integral membrane phosphatases, can hydrolyze FPP into farnesol. Overexpression of FPP synthase (IspA) and PgpB, along with a heterologous mevalonate pathway, enabled recombinant E. coli to produce 526.1 mg/L of farnesol. This result indicates that the phosphatases PgpB and YbjG can be used to construct a novel farnesol synthesis pathway for mass production in E. coli.
authors.affiliation: Division of Applied Life Science (BK21 Plus), PMBBRC, Gyeongsang National University, Jinju, Republic of Korea.|School of Biology and Basic Medical Sciences, Soochow University, Suzhou, China.
authors.forename: Chonglong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Division of Applied Life Science (BK21 Plus), PMBBRC, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Ju-Eon
authors.initials: JE
authors.lastname: Park
authors.affiliation: Industrial Biotechnology Research Center, KRIBB, Daejeon, Republic of Korea. choi4162@kribb.re.kr.
authors.forename: Eui-Sung
authors.initials: ES
authors.lastname: Choi
authors.affiliation: Division of Applied Life Science (BK21 Plus), PMBBRC, Gyeongsang National University, Jinju, Republic of Korea. swkim@gnu.ac.kr.
authors.forename: Seon-Won
authors.initials: SW
authors.lastname: Kim
mesh_terms: D053898:Biosynthetic Pathways; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005204:Farnesol / Q000378:metabolism*; D006868:Hydrolysis; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008798:Mevalonic Acid / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D010711:Phosphatidate Phosphatase / Q000235:genetics* / Q000378:metabolism; D011106:Polyisoprenyl Phosphates / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012717:Sesquiterpenes / Q000737:chemistry; D015854:Up-Regulation
chemical_list: D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C000608554:PgpB protein, E coli; D011106:Polyisoprenyl Phosphates; D011994:Recombinant Proteins; D012717:Sesquiterpenes; C585154:YbjG protein, E coli; D005204:Farnesol; C004808:farnesyl pyrophosphate; D010711:Phosphatidate Phosphatase; D008798:Mevalonic Acid
keywords: Escherichia coli; Farnesol; PgpB; Phosphatase; YbjG