title: Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ.
abstract: Hair follicle morphogenesis is first induced by epithelial-mesenchymal interactions in the developing embryo. In the hair follicle, various stem-cell populations are maintained in specialized niches to promote repetitive hair follicle-morphogenesis, which is observed in the variable lower region of the hair follicle as a postnatal hair cycle. In contrast, the genesis of most organs is induced only once during embryogenesis. We developed a novel bioengineering technique, the Organ Germ Method, that employs three-dimensional stem cell culture for regenerating various organs and reproducing embryonic organogenesis. In this chapter, we describe a protocol for hair follicle germ reconstitution using adult follicle-derived epithelial stem cells and dermal papilla cells with intracutaneous transplantation of the bioengineered hair-follicle organ germ. This protocol can be useful not only for the clinical study of hair regeneration but also for studies of stem cell biology and organogenesis.
authors.affiliation: Center for Developmental Biology, RIKEN, Kobe, Hyogo, 650-0047, Japan.|Organ Technologies Inc., Tokyo, 105-0001, Japan.
authors.forename: Katsunari
authors.initials: K
authors.lastname: Tezuka
authors.affiliation: Center for Developmental Biology, RIKEN, Kobe, Hyogo, 650-0047, Japan.|Organ Technologies Inc., Tokyo, 105-0001, Japan.|Department of Regenerative Medicine, Plastic and Reconstructive Surgery, Kitasato University of Medicine, Sagamihara, Kanagawa, 252-0374, Japan.
authors.forename: Koh-Ei
authors.initials: KE
authors.lastname: Toyoshima
authors.affiliation: Center for Developmental Biology, RIKEN, Kobe, Hyogo, 650-0047, Japan. t-tsuji@cdb.riken.jp.|Organ Technologies Inc., Tokyo, 105-0001, Japan. t-tsuji@cdb.riken.jp.|Department of Regenerative Medicine, Plastic and Reconstructive Surgery, Kitasato University of Medicine, Sagamihara, Kanagawa, 252-0374, Japan. t-tsuji@cdb.riken.jp.|Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, Japan. t-tsuji@cdb.riken.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D001698:Biomedical Engineering; D002469:Cell Separation; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology; D004847:Epithelial Cells / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology* / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014738:Vibrissae
keywords: Epithelial–mesenchymal interaction; Hair follicle; Organ Germ Method; Stem cells