title: Testing Transgenic Aspen Plants with bar Gene for Herbicide Resistance under Semi-natural Conditions.
abstract: Obtaining herbicide resistant plants is an important task in the genetic engineering of forest trees. Transgenic European aspen plants (Populus tremula L.) expressing the bar gene for phosphinothricin resistance have been produced using Agrobacterium tumefaciens-mediated transformation. Successful genetic transformation was confirmed by PCR analysis for thirteen lines derived from two elite genotypes. In 2014-2015, six lines were evaluated for resistance to herbicide treatment under semi-natural conditions. All selected transgenic lines were resistant to the herbicide Basta at doses equivalent to 10 l/ha (twofold normal field dosage) whereas the control plants died at 2.5 l/ha. Foliar NH4-N concentrations in transgenic plants did not change after treatment. Extremely low temperatures in the third ten-day period of October 2014 revealed differences in freeze tolerance between the lines obtained from Pt of f2 aspen genotypes. Stable expression of the bar gene after overwintering outdoors was confirmed by RT-PCR. On the basis of the tests, four transgenic aspen lines were selected. The bar gene could be used for retransformation of transgenic forest trees expressing valuable traits, such as increased productivity.
authors.affiliation: Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Science av., 6, 142290, Pushchino, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Lebedev
authors.affiliation: Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Science av., 6, 142290, Pushchino, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Faskhiev
authors.affiliation: Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Science av., 6, 142290, Pushchino, Russia.
authors.forename: N P
authors.initials: NP
authors.lastname: Kovalenko
authors.affiliation: Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Science av., 6, 142290, Pushchino, Russia.
authors.forename: K A
authors.initials: KA
authors.lastname: Shestibratov
authors.affiliation: Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Science av., 6, 142290, Pushchino, Russia ; Lomonosov Moscow State University, Leninskie Gory, 1/51, 119991, Moscow, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Miroshnikov
keywords: aspen; bar gene; herbicide resistance; phosphinothricin; transgenic plants