title: Exposure to Sevoflurane Affects the Development of Parvalbumin Interneurons in the Main Olfactory Bulb in Mice.
abstract: Sevoflurane is widely used in adult and pediatric patients during clinical surgeries. Although studies have shown that exposure to sevoflurane impairs solfactory memory after an operation, the neuropathological changes underlying this effect are not clear. This study detected the effect of sevoflurane exposure on the development of calcium-binding proteins-expressing interneurons in the main olfactory bulb (MOB). We exposed neonatal mice to 2% sevoflurane at two different developmental time points and found that exposing mice to sevoflurane at postnatal day (PD) 7 significantly decreased the expression of GAD67 and parvalbumin (PV) in the olfactory bulb (OB) but did not alter the expression of calretinin (CR) or calbindin D28k (CB). The number and dendritic morphology of PV-expressing interneurons in the MOB were impaired by exposure to sevoflurane at PD7. However, exposure to sevoflurane at PD10 had no effect on calcium-binding protein expression or the number and dendritic morphology of PV-expressing interneurons in the MOB. These results suggest that exposing neonatal mice to sevoflurane during a critical period of olfactory development affects the development of PV-expressing interneurons in the MOB.
authors.affiliation: Institute of Neuroscience and Anatomy, School of Medicine, Zhejiang University Hangzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Anatomy and K.K. Leung Brain Research Center, Fourth Military Medical University Xi'an, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Neurobiology and Collaborative Innovation Centre for Brain Science, Fourth Military Medical University Xi'an, China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Anesthesiology, School of Stomatology, The Fourth Military Medical University Xi'an, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Lu
authors.affiliation: Institute of Neuroscience and Anatomy, School of Medicine, Zhejiang University Hangzhou, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Neurobiology and Collaborative Innovation Centre for Brain Science, Fourth Military Medical University Xi'an, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Luo
authors.affiliation: Department of Neurobiology and Collaborative Innovation Centre for Brain Science, Fourth Military Medical University Xi'an, China.
authors.forename: Shengxi
authors.initials: S
authors.lastname: Wu
authors.affiliation: Institute of Neuroscience and Anatomy, School of Medicine, Zhejiang University Hangzhou, China.
authors.forename: Shucai
authors.initials: S
authors.lastname: Ling
keywords: CB; CR; PV; olfactory bulb; sevoflurane