title: CB5C affects the glucosinolate profile in Arabidopsis thaliana.
abstract: Cytochrome b5 (CB5) proteins are small heme-binding proteins, that influence cytochrome P450 activity. While only one CB5 isoform is found in mammals, higher plants have several isoforms of these proteins. The roles of the many CB5 isoforms in plants remain unknown. We hypothesized that CB5 proteins support the cytochrome P450 enzymes of plant specialized metabolism and found CB5C from Arabidopsis thaliana to co-express with glucosinolate biosynthetic genes. We characterized the glucosinolate profiles of 2 T-DNA insertion mutants of CB5C, and found that long-chained aliphatic glucosinolates were reduced in one of the mutant lines - a phenotype that was exaggerated upon methyl-jasmonate treatment. These results support the hypothesis, that CB5C influences glucosinolate biosynthesis, however, the mode of action remains unknown. Furthermore, the mutants differed in their biomass response to methyl jasmonate treatment. Thereby, our results highlight the varying effects of T-DNA insertion sites, as the 2 analyzed alleles show different phenotypes.
authors.affiliation: a Department of Plant and Environmental Sciences, DynaMo Center of Excellence & Copenhagen Plant Science Center , University of Copenhagen , Denmark.
authors.forename: Daniel
authors.initials: D
authors.lastname: Vik
authors.affiliation: a Department of Plant and Environmental Sciences, DynaMo Center of Excellence & Copenhagen Plant Science Center , University of Copenhagen , Denmark.
authors.forename: Christoph
authors.initials: C
authors.lastname: Crocoll
authors.affiliation: b Department of Plant Molecular Biology , University of Lausanne , Switzerland.
authors.forename: Tonni Grube
authors.initials: TG
authors.lastname: Andersen
authors.affiliation: a Department of Plant and Environmental Sciences, DynaMo Center of Excellence & Copenhagen Plant Science Center , University of Copenhagen , Denmark.
authors.forename: Meike
authors.initials: M
authors.lastname: Burow
authors.affiliation: a Department of Plant and Environmental Sciences, DynaMo Center of Excellence & Copenhagen Plant Science Center , University of Copenhagen , Denmark.
authors.forename: Barbara Ann
authors.initials: BA
authors.lastname: Halkier
mesh_terms: D000085:Acetates / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D015786:Cytochromes b5 / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D005961:Glucosinolates / Q000378:metabolism*; D054883:Oxylipins / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D004269:DNA, Bacterial; D005961:Glucosinolates; D054883:Oxylipins; C036483:T-DNA; C072239:methyl jasmonate; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System
keywords: Arabidopsis; cytochrome P450; cytochrome b5; glucosinolates; jasmonate; specialized metabolism