title: Infective Endocarditis Caused by Panton-Valentine Leukocidin-producing Methicillin-susceptible Staphylococcus aureus Identified by the Broad-range PCR Method.
abstract: A 76-year-old man was admitted to a community hospital due to a persistent high fever. He became afebrile after the administration of broad-spectrum antibiotics, but developed heart failure due to progressive aortic and mitral valve insufficiency and was transferred to our hospital. Although sequential blood cultures were negative, a broad-range polymerase chain reaction targeting the bacterial 16S-rRNA gene followed by the direct sequencing of whole blood revealed spa(+), mecA(-) and Panton-Valentine leukocidin (PVL)(+). He was finally diagnosed with infective endocarditis (IE) caused by PVL-producing methicillin-susceptible Staphylococcus aureus (MSSA), and underwent cardiac surgery. This is the first reported case of IE due to MSSA producing PVL.
authors.affiliation: Department of Cardiology and Nephrology, Mie University Graduate School of Medicine, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakajima
authors.forename: Kaoru
authors.initials: K
authors.lastname: Dohi
authors.forename: Masaki
authors.initials: M
authors.lastname: Tanabe
authors.forename: Akiko
authors.initials: A
authors.lastname: Nakamura
authors.forename: Shinji
authors.initials: S
authors.lastname: Kanemitsu
authors.forename: Hideo
authors.initials: H
authors.lastname: Wada
authors.forename: Norikazu
authors.initials: N
authors.lastname: Yamada
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nobori
authors.forename: Hideto
authors.initials: H
authors.lastname: Shinpo
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ito
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001427:Bacterial Toxins / Q000096:biosynthesis*; D004697:Endocarditis, Bacterial / Q000188:drug therapy / Q000382:microbiology*; D005098:Exotoxins / Q000096:biosynthesis*; D006801:Humans; D007956:Leukocidins / Q000096:biosynthesis*; D008297:Male; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin