title: Assessment of Ovarian Cancer Tumors Treated with Intraperitoneal Cisplatin Therapy by Nanoscopic X-ray Fluorescence Imaging.
abstract: Ovarian cancer is amongst the most common types of cancer in women, with a relatively low overall cure rate of approximately 30%. This is therefore an important incentive to urge for further research in order to maximize the chances of survival for these patients. Intraperitoneal chemotherapy with Cisplatin is an effective treatement for ovarian cancer; however, many questions still remain concerning the ideal treatment protocol and tumor resistance towards the drug, which should be resolved for optimal application of this therapy. For the first time in-vivo grown tumors treated with both hyper- and normothermic intraperitoneal chemotherapy have been studied using nano-XRF spectroscopy to examine the platinum (Pt) distribution within the analyzed tissues. These measurements prove Pt resides predominantly outsides the cancer cells in the stroma of the tissue. These findings indicate the resistance mechanism of the cancer cells prevents Cisplatin from diffusing through their cell membranes. This is an important addition to the existing knowledge on the resistance mechanism providing insights which might help to overcome this effect. In our aim to find the optimal treatment protocol, no significant differences were found between the two examined procedures. A more extensive data set will be needed to draw definite conclusions.
authors.affiliation: X-ray Microspectroscopy and Imaging Group (XMI), Ghent University, Krijgslaan 281 S12, B-9000 Ghent, Belgium.
authors.forename: Brecht
authors.initials: B
authors.lastname: Laforce
authors.affiliation: Department of Surgery, Laboratory of Experimental Surgery, Ghent University Hospital, Ghent B-9000, Belgium.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Carlier
authors.affiliation: X-ray Microspectroscopy and Imaging Group (XMI), Ghent University, Krijgslaan 281 S12, B-9000 Ghent, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Vekemans
authors.affiliation: European Synchrotron Radiation Facility (ESRF), FR-38043 Grenoble Cedex, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Villanova
authors.affiliation: European Synchrotron Radiation Facility (ESRF), FR-38043 Grenoble Cedex, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Tucoulou
authors.affiliation: Department of Surgery, Laboratory of Experimental Surgery, Ghent University Hospital, Ghent B-9000, Belgium.
authors.forename: Wim
authors.initials: W
authors.lastname: Ceelen
authors.affiliation: X-ray Microspectroscopy and Imaging Group (XMI), Ghent University, Krijgslaan 281 S12, B-9000 Ghent, Belgium.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Vincze
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000627:therapeutic use*; D003198:Computer Simulation; D057225:Data Mining; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D005453:Fluorescence; D007274:Injections, Intraperitoneal; D008819:Mice, Nude; D009010:Monte Carlo Method; D036103:Nanotechnology; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000188:drug therapy* / Q000473:pathology; D010984:Platinum / Q000627:therapeutic use; D014965:X-Rays
chemical_list: D010984:Platinum; D002945:Cisplatin