title: Immunomodulatory Effects of Lactobacillus plantarum Lp62 on Intestinal Epithelial and Mononuclear Cells.
abstract: Probiotic lactic acid bacteria are known for their ability to modulate the immune system. They have been shown to inhibit inflammation in experiments with animal models, cell culture, and clinical trials. The objective of this study was to elucidate the anti-inflammatory potential of Lactobacillus plantarum Lp62, isolated from cocoa fermentation, in a cell culture model. Lp62 inhibited IL-8 production by Salmonella Typhi-stimulated HT-29 cells and prevented the adhesion of pathogens to these epithelial cells. The probiotic strain was able to modulate TNF-α, IL1-β, and IL-17 secretion by J774 macrophages. J774 activation was reduced by coincubation with Lp62. PBMC culture showed significantly higher levels of CD4(+)CD25(+) T lymphocytes following treatment with Lp62. Probiotics also induced increased IL-10 secretion by mononuclear cells. L. plantarum Lp62 was able to inhibit inflammatory stimulation in epithelial cells and macrophages and activated a tolerogenic profile in mononuclear cells of healthy donors. These results indicate this strain for a possible application in the treatment or prevention of inflammatory diseases.
authors.affiliation: State University of Feira de Santana, Transnordestina Avenue S/N, 44030-900 Feira de Santana, BA, Brazil.
authors.forename: Thalis
authors.identifier: 0000-0002-6603-3409
authors.initials: T
authors.lastname: Ferreira Dos Santos
authors.affiliation: State University of Santa Cruz, Highway Ilhéus-Itabuna, km 16 S/N, 45662-900 Ilhéus, BA, Brazil.
authors.forename: Tauá
authors.initials: T
authors.lastname: Alves Melo
authors.affiliation: State University of Santa Cruz, Highway Ilhéus-Itabuna, km 16 S/N, 45662-900 Ilhéus, BA, Brazil.
authors.forename: Milena Evangelista
authors.initials: ME
authors.lastname: Almeida
authors.affiliation: State University of Santa Cruz, Highway Ilhéus-Itabuna, km 16 S/N, 45662-900 Ilhéus, BA, Brazil.
authors.forename: Rachel
authors.initials: R
authors.lastname: Passos Rezende
authors.affiliation: State University of Santa Cruz, Highway Ilhéus-Itabuna, km 16 S/N, 45662-900 Ilhéus, BA, Brazil.
authors.forename: Carla Cristina
authors.identifier: 0000-0003-3549-4736
authors.initials: CC
authors.lastname: Romano
mesh_terms: D000818:Animals; D002460:Cell Line; D000069196:Gastrointestinal Microbiome / Q000276:immunology; D019073:HT29 Cells; D006801:Humans; D007165:Immunosuppression Therapy / Q000379:methods; D016753:Interleukin-10 / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000276:immunology* / Q000382:microbiology*; D048191:Lactiplantibacillus plantarum / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D051379:Mice; D019936:Probiotics / Q000494:pharmacology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000382:microbiology
chemical_list: D016753:Interleukin-10