title: Deferoxamine ameliorates hepatosteatosis via several mechanisms in ob/ob mice.
abstract: Hepatic iron accumulation may be responsible for the pathology of nonalcoholic fatty liver disease (NAFLD), which is both increasingly prevalent in conjunction with obesity and associated with comorbidities. The efficacy of iron reduction therapies, such as phlebotomy or dietary iron restriction, has been demonstrated in patient and animal models, including models of diabetes and obesity; however, the effects on and exact mechanisms responsible for iron depletion in NAFLD have not been clearly elucidated. Our study investigated the role of iron depletion by deferoxamine (DFO) treatment of ob/ob mice with hepatic steatosis. We found that DFO reduced hepatic iron deposition and regulated intracellular iron concentration in a homeostatic process following 15 days of treatment. Compared with vehicle treatment, DFO significantly improved hepatic steatosis by upregulating proteins related to lipid metabolism. Meanwhile, the reduction of free radical formation and proinflammatory cytokines, as well as the increase of hypoxia-inducible factor-1α pathway proteins and Bcl2/Bax ratio, further indicated that DFO was effective for liver protection and hepatic adaptation. These findings show that the intraperitoneal delivery of DFO provides a potential means of both preventing the progression of NAFLD and accelerating healing of hepatic steatosis, with the potential for rapid clinical application.
authors.affiliation: College of Life and Health Sciences, Northeastern University, Shenyang, P. R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Xue
authors.affiliation: College of Life and Health Sciences, Northeastern University, Shenyang, P. R. China.
authors.forename: Di
authors.initials: D
authors.lastname: Chen
authors.affiliation: College of Life and Health Sciences, Northeastern University, Shenyang, P. R. China.
authors.forename: Yan-Ke
authors.initials: YK
authors.lastname: Zhong
authors.affiliation: College of Life and Health Sciences, Northeastern University, Shenyang, P. R. China.
authors.forename: Zhen-Diao
authors.initials: ZD
authors.lastname: Zhou
authors.affiliation: College of Life and Health Sciences, Northeastern University, Shenyang, P. R. China.
authors.forename: Shi-Xin
authors.initials: SX
authors.lastname: Fang
authors.affiliation: College of Life and Health Sciences, Northeastern University, Shenyang, P. R. China.
authors.forename: Ming-Yao
authors.initials: MY
authors.lastname: Li
authors.affiliation: College of Life and Health Sciences, Northeastern University, Shenyang, P. R. China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Guo
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003676:Deferoxamine / Q000494:pharmacology / Q000627:therapeutic use*; D005247:Feeding Behavior / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D007249:Inflammation / Q000473:pathology; D042541:Intracellular Space / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D008820:Mice, Obese; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D051017:Apoptosis Regulatory Proteins; C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007501:Iron; D003676:Deferoxamine
keywords: deferoxamine; iron; nonalcoholic fatty liver disease; ob/ob mice