title: Growth and Detachment of 5 Helix DNA Ribbons.
abstract: We report on the concentration-dependent surface-assisted growth and time-temperature-dependent detachment of one-dimensional 5 helix DNA ribbons (5HR) on a mica substrate. The growth coverage ratio was determined by varying the concentration of the 5HR strands in a test tube, and the detachment rate of 5HR on mica was determined by varying the incubation time at a fixed temperature on a heat block. The topological changes in the concentration-dependent attachment and the time-temperature-dependent detachment for 5HR on mica were observed via atomic force microscopy. The observations indicate that 5HR started to grow on mica at ~10 nM and provided full coverage at ~50 nM. In contrast, 5HR at 65 °C started to detach from mica after 5 min and was completely removed after 10 min. The growth and detachment coverage show a sinusoidal variation in the growth ratio and a linear variation with a rate of detachment of 20%/min, respectively. The physical parameters that control the stability of the DNA structures on a given substrate should be studied to successfully integrate DNA structures for physical and chemical applications.
authors.forename: Saima
authors.initials: S
authors.lastname: Bashar
authors.forename: Si Un
authors.initials: SU
authors.lastname: Hwang
authors.forename: Junwye
authors.initials: J
authors.lastname: Lee
authors.forename: Rashid
authors.initials: R
authors.lastname: Amin
authors.forename: Sreekantha Reddy
authors.initials: SR
authors.lastname: Dugasani
authors.forename: Tai Hwan
authors.initials: TH
authors.lastname: Ha
authors.forename: Sung Ha
authors.initials: SH
authors.lastname: Park
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D000538:Aluminum Silicates; D004247:DNA; C011934:mica