title: XTT Assay of Antifungal Activity.
abstract: XTT assay is acolorimetric method that uses the tetrazolium dye, 2,3-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanilide (XTT) to quantify cell-mediated damage to fungi. Actively respiring fungal cells convert the water-soluble XTT to a water-soluble, orange colored formazan product (Meshulamet al., 1995). Here, we describe the protocol that measures the ability of plasmacytoid dendritic cells (pDCs) to exert antifungal activity. This approach was first established with human polymorphonuclear cells (PMN) by Meshulamet al.(1995) and then adapted to pDC by Ramirez-Ortizet al.(2011) and Loureset al.(2015). It can be modified for use with other effector cells and to test compounds for antifungal activity.
authors.affiliation: Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America, Department of Immunology, Institute of Biomedical Sciences, São Paulo University, São Paulo, Brazil.
authors.forename: Flávio V
authors.initials: FV
authors.lastname: Loures
authors.affiliation: Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Levitz