title: Chitosan Nanofibers for Specific Capture and Nondestructive Release of CTCs Assisted by pCBMA Brushes.
abstract: Over the last decade, significant progress has been made to develop sensitive devices for the capture of circulating tumor cells (CTCs) from blood of cancer patients. However, simple capture and counting of CTCs cannot provide effective information for understanding the biology of them. In this work, a functional biointerface is fabricated for specific capture and nondestructive release of CTCs from blood samples. A nanostrucure of porous network based on chitosan nanofibers is fabricated by electrospinning, to mimic the function of extracellular matrices, and then the poly(carboxybetaine methacrylate) (pCBMA) brushes integrating onto nanofiber interface provide the effect of interfacial properties to control nonspecific cell adhesion and the multivalent immobilization of aptamers to induce high efficient and specific CTC capture. Furthermore, a complementary sequence is used to efficiently hybridize with the aptamer to achieve nondestructive release of the captured target cells, assisted by the flexible space provided by pCBMA brushes. This work also shows how nanostructure and the interface molecules regulate the morphology of the captured CTCs, and reveals the importance of the controllable cell morphology on biointerface for an effective nondestructive release of the captured CTCs.
authors.affiliation: Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.affiliation: Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China. jewang2012@sinano.ac.cn.|University of Chinese Academy of Sciences, Beijing, 100049, China. jewang2012@sinano.ac.cn.
authors.forename: Jine
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Xinpan
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China. rjpei2011@sinano.ac.cn.|University of Chinese Academy of Sciences, Beijing, 100049, China. rjpei2011@sinano.ac.cn.
authors.forename: Renjun
authors.initials: R
authors.lastname: Pei
mesh_terms: D001622:Betaine / Q000737:chemistry*; D002460:Cell Line; D048430:Cell Shape; D048271:Chitosan / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D011109:Polymethacrylic Acids / Q000737:chemistry*
chemical_list: D011109:Polymethacrylic Acids; C517178:polycarboxybetaine methacrylate; D001622:Betaine; D048271:Chitosan
keywords: CTCs; antifouling; chitosan; nanofibers; nondestructive release