title: Pulse Duplicator Hydrodynamic Testing of Bioengineered Biological Heart Valves.
abstract: There are many heart valve replacements currently available on the market; however, these devices are not ideal for pediatric patients with congenital heart valve defects. Decellularized valve substitutes offer potential for improved clinical outcomes and require pre-clinical testing guidelines and testing systems suitable for non-crosslinked, biological heart valves. The objective of this study was to assess the hydrodynamic performance of intact, bioengineered pulmonary valves using a custom pulse duplicator capable of testing intact biological valved conduits. The mechanical behavior of valve associated sinus and arterial tissue was also evaluated under biaxial loading. Cryopreserved, decellularized, extracellular matrix (ECM) conditioned and glutaraldehyde fixed valves showed reduced pressure gradients and increased effective orifice area for decellularized and ECM conditioned valves. ECM conditioning resulted in increased elastic modulus but decreased stretch in circumferential and longitudinal directions under biaxial loading. Overall, decellularization and ECM conditioning did not compromise the scaffolds, which exhibited satisfactory bench top performance.
authors.affiliation: Cardiac Regenerative Surgery Research Laboratories of The Ward Family Heart Center, Children's Mercy Kansas City, 2401 Gillham Road, Kansas City, MO, 64108, USA.
authors.forename: Eric E
authors.initials: EE
authors.lastname: Buse
authors.affiliation: Cardiac Regenerative Surgery Research Laboratories of The Ward Family Heart Center, Children's Mercy Kansas City, 2401 Gillham Road, Kansas City, MO, 64108, USA.
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Hilbert
authors.affiliation: Cardiac Regenerative Surgery Research Laboratories of The Ward Family Heart Center, Children's Mercy Kansas City, 2401 Gillham Road, Kansas City, MO, 64108, USA.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Hopkins
authors.affiliation: Cardiac Regenerative Surgery Research Laboratories of The Ward Family Heart Center, Children's Mercy Kansas City, 2401 Gillham Road, Kansas City, MO, 64108, USA. glconverse@cmh.edu.
authors.forename: Gabriel L
authors.initials: GL
authors.lastname: Converse
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D019544:Equipment Failure Analysis / Q000379:methods*; D006350:Heart Valve Prosthesis; D057446:Hydrodynamics; D013552:Swine; D023822:Tissue Engineering
keywords: Decellularization; Pre-regulatory testing; Pulmonary heart valve; Tissue engineering