title: Long-Standing Motor and Sensory Recovery following Acute Fibrin Sealant Based Neonatal Sciatic Nerve Repair.
abstract: Brachial plexus lesion results in loss of motor and sensory function, being more harmful in the neonate. Therefore, this study evaluated neuroprotection and regeneration after neonatal peripheral nerve coaptation with fibrin sealant. Thus, P2 neonatal Lewis rats were divided into three groups: AX: sciatic nerve axotomy (SNA) without treatment; AX+FS: SNA followed by end-to-end coaptation with fibrin sealant derived from snake venom; AX+CFS: SNA followed by end-to-end coaptation with commercial fibrin sealant. Results were analyzed 4, 8, and 12 weeks after lesion. Astrogliosis, microglial reaction, and synapse preservation were evaluated by immunohistochemistry. Neuronal survival, axonal regeneration, and ultrastructural changes at ventral spinal cord were also investigated. Sensory-motor recovery was behaviorally studied. Coaptation preserved synaptic covering on lesioned motoneurons and led to neuronal survival. Reactive gliosis and microglial reaction decreased in the same groups (AX+FS, AX+CFS) at 4 weeks. Regarding axonal regeneration, coaptation allowed recovery of greater number of myelinated fibers, with improved morphometric parameters. Preservation of inhibitory synaptic terminals was accompanied by significant improvement in the motor as well as in the nociceptive recovery. Overall, the present data suggest that acute repair of neonatal peripheral nerves with fibrin sealant results in neuroprotection and regeneration of motor and sensory axons.
authors.affiliation: Department of Tropical Diseases, Botucatu Medical School, São Paulo State University (UNESP), 18618-000 Botucatu, SP, Brazil; Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), 18610-307 Botucatu, SP, Brazil.
authors.forename: Natalia
authors.identifier: 0000-0001-9345-085X
authors.initials: N
authors.lastname: Perussi Biscola
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, University of Campinas, 13083-970 Campinas, SP, Brazil.
authors.forename: Luciana
authors.identifier: 0000-0003-1942-800X
authors.initials: L
authors.lastname: Politti Cartarozzi
authors.affiliation: Department of Tropical Diseases, Botucatu Medical School, São Paulo State University (UNESP), 18618-000 Botucatu, SP, Brazil; Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), 18610-307 Botucatu, SP, Brazil.
authors.forename: Rui Seabra
authors.initials: RS
authors.lastname: Ferreira Junior
authors.affiliation: Department of Tropical Diseases, Botucatu Medical School, São Paulo State University (UNESP), 18618-000 Botucatu, SP, Brazil; Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), 18610-307 Botucatu, SP, Brazil.
authors.forename: Benedito
authors.identifier: 0000-0002-9855-5594
authors.initials: B
authors.lastname: Barraviera
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, University of Campinas, 13083-970 Campinas, SP, Brazil.
authors.forename: Alexandre
authors.identifier: 0000-0003-4224-4575
authors.initials: A
authors.lastname: Leite Rodrigues de Oliveira
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival / Q000502:physiology; D015718:Fibrin Tissue Adhesive / Q000008:administration & dosage*; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011917:Rats, Inbred Lew; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology*; D012584:Sciatic Nerve / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D020426:Sciatic Neuropathy / Q000188:drug therapy* / Q000473:pathology; D012910:Snake Venoms; D013997:Time Factors
chemical_list: D015718:Fibrin Tissue Adhesive; D012910:Snake Venoms