title: TaTypA, a Ribosome-Binding GTPase Protein, Positively Regulates Wheat Resistance to the Stripe Rust Fungus.
abstract: Tyrosine phosphorylation protein A (TypA/BipA) belongs to the ribosome-binding GTPase superfamily. In many bacterial species, TypA acts as a global stress and virulence regulator and also mediates resistance to the antimicrobial peptide bactericidal permeability-increasing protein. However, the function of TypA in plants under biotic stresses is not known. In this study, we isolated and functionally characterized a stress-responsive TypA gene (TaTypA) from wheat, with three copies located on chromosomes 6A, 6B, and 6D, respectively. Transient expression assays indicated chloroplast localization of TaTypA. The transcript levels of TaTypA were up-regulated in response to treatment with methyl viologen, which induces reactive oxygen species (ROS) in chloroplasts through photoreaction, cold stress, and infection by an avirulent strain of the stripe rust pathogen. Knock down of the expression of TaTypA through virus-induced gene silencing decreased the resistance of wheat to stripe rust accompanied by weakened ROS accumulation and hypersensitive response, an increase in TaCAT and TaSOD expression, and an increase in pathogen hyphal growth and branching. Our findings suggest that TaTypA contributes to resistance in an ROS-dependent manner.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Thwin
authors.initials: T
authors.lastname: Myo
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Dingyun
authors.initials: D
authors.lastname: Lan
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Tuo
authors.initials: T
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Song
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Kang
keywords: Puccinia striiformis f. sp. tritici; ROS; Triticum aestivum; TypA; tyrosine phosphorylation; virus-induced gene silencing