title: Rapid endoglin determination in serum samples using an amperometric magneto-actuated disposable immunosensing platform.
abstract: A sensitive and rapid method for the determination of the clinically relevant biomarker human endoglin (CD105) in serum samples is presented, involving a magneto-actuated immunoassay and amperometric detection at disposable screen-printed carbon electrodes (SPCEs). Micro-sized magnetic particles were modified with a specific antibody to selectively capture the target protein which was further sandwiched with a secondary HRP-labeled antibody. The immunocomplexes attached to the magnetic carriers were amperometrically detected at SPCEs using the hydroquinone (HQ)/H2O2/HRP system. The magneto-actuated immunosensing platform was able to detect 5 pmoles of endoglin (in 25μL of sample, 0.2μM) in 30min providing statistically similar results to those obtained using a commercial ELISA kit for the determination of endogenous content of endoglin in human serum samples.
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain. Electronic address: rebeca.magnolia@gmail.com.
authors.forename: Rebeca M
authors.initials: RM
authors.lastname: Torrente-Rodríguez
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain. Electronic address: susanacr@quim.ucm.es.
authors.forename: Susana
authors.initials: S
authors.lastname: Campuzano
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain. Electronic address: victor_lega90@hotmail.com.
authors.forename: Víctor
authors.initials: V
authors.lastname: Ruiz-Valdepeñas-Montiel
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain. Electronic address: mpedrero@quim.ucm.es.
authors.forename: María
authors.initials: M
authors.lastname: Pedrero
authors.affiliation: Department of Pathology, Hospital Clínico San Carlos, 28040 Madrid, Spain. Electronic address: jmariajesus.fernandez@salud.madrid.org.
authors.forename: M Jesús
authors.initials: MJ
authors.lastname: Fernández-Aceñero
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain. Electronic address: rbarderas@quim.ucm.es.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Barderas
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain. Electronic address: pingarro@quim.ucm.es.
authors.forename: José M
authors.initials: JM
authors.lastname: Pingarrón
mesh_terms: D000906:Antibodies / Q000097:blood / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D000071063:Endoglin / Q000097:blood* / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D006873:Hydroquinones / Q000097:blood / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D008280:Magnetics / Q000379:methods; D044967:Serum / Q000737:chemistry*
chemical_list: D000906:Antibodies; D000071063:Endoglin; D006873:Hydroquinones; D002244:Carbon; D006861:Hydrogen Peroxide; C031927:hydroquinone
keywords: Amperometric immunosensor; CD105; Magnetic beads; Screen-printed electrodes; Serum samples