title: Prevalence of Chlamydia trachomatis Genotypes in Men Who Have Sex with Men and Men Who Have Sex with Women Using Multilocus VNTR Analysis-ompA Typing in Guangzhou, China.
abstract: BACKGROUND
  Chlamydia trachomatis is one of the most prevalent bacterial sexually transmitted infection in China. Although C. trachomatis genotypes can be discriminated by outer membrane protein gene (ompA) sequencing, currently available methods have limited resolutions. This study used a high-resolution genotyping method, namely, multilocus variable number tandem-repeat analysis with ompA sequencing (MLVA)-ompA, to investigate the local epidemiology of C. trachomatis infections among men who have sex with men (MSM) and men who have sex with women (MSW) attending a sexually transmitted diseases (STD) clinic in Guangzhou, China.
  
  
  METHODS
  Rectal specimens from MSM and urethral specimens from MSW were collected between January 2013 and July 2014 at the Guangdong Provincial Center STD clinic. The specimens were sent to the laboratory for analyses. All specimens that were tested positive for C. trachomatis by the commercial nucleic acid amplification tests were genotyped by MLVA-ompA.
  
  
  RESULTS
  Fifty-one rectal specimens from MSM and 96 urethral specimens from MSW were identified with C. trachomatis. One hundred and forty-four of the 147 specimens were fully genotyped by MLVA-ompA. Rectal specimens from MSM were divided into four ompA genotypes and urethral specimens from MSW into nine genotypes. No mixed infections were found among all specimens. The most frequent genotypes were D, G, J, E and F. All specimens were further divided into 46 types after ompA genotyping was combined with MLVA. Genotypes D-8.7.1 and G-3.4a.3 were the most frequent among MSM, whereas genotypes D-3.4a.4, E-8.5.1, F-8.5.1, and J-3.4a.2 were the most frequent subtypes among MSW. The discriminatory index D was 0.90 for MLVA, 0.85 for ompA, and 0.95 for MLVA-ompA.
  
  
  CONCLUSIONS
  The most prevalent MLVA-ompA genotypes were significantly different between MSM and MSW from Guangzhou, China. Moreover, MLVA-ompA represented a more favorable degree of discrimination than ompA and could be a reliable complement for ompA for the routine subtypes of C. trachomatis.
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Qin
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.
authors.forename: Heping
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.|Department of Laboratory Medicine, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.
authors.forename: Xuqi
authors.initials: X
authors.lastname: Ren
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Huang
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.
authors.forename: Shujie
authors.initials: S
authors.lastname: Huang
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.
authors.forename: Xingzhong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.
authors.forename: Weiying
authors.initials: W
authors.lastname: Zeng
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.
authors.forename: Jiangli
authors.initials: J
authors.lastname: Ou
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.
authors.forename: Yinyuan
authors.initials: Y
authors.lastname: Lan
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.
authors.forename: Sanmei
authors.initials: S
authors.lastname: Tang
mesh_terms: D000293:Adolescent; D000328:Adult; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D002681:China / Q000453:epidemiology; D002690:Chlamydia Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D015870:Gene Expression; D005838:Genotype; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D012007:Rectum / Q000382:microbiology; D012747:Sexual Partners; D020080:Tandem Repeat Sequences; D014521:Urethra / Q000382:microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins