title: A novel intravenous vehicle for preclinical cardiovascular screening of small molecule drug candidates in rat.
abstract: Screening novel, poorly soluble small-molecule candidates for cardiovascular liabilities represents a key challenge in early drug discovery. This report describes a novel vehicle composed of 20% N,N-Dimethylacetamide (DMA)/40% Propylene glycol (PG)/40% Polyethylene Glycol (PEG-400) (DPP) for administration of new chemical entities (NCEs) by slow intravenous (i.v.) infusion in a preclinical anesthetized rat model. The vehicle was designed considering both available excipient safety information and solubilization potential for poorly soluble NCEs. DPP solubilized 11 drugs, 8 of which were insoluble in 5% dextrose in water (D5W), and 5 insoluble in PEG-400 to a target concentration of 30mg/mL. DPP elicits no adverse cardiovascular responses in the anesthetized rat model despite containing 40% PEG-400, a commonly used organic solvent which elicits hypertension and bradycardia that often confounds interpretation of drug effects. Three compounds demonstrating adequate solubility in both DPP and D5W were screened in the anesthetized rat model. When normalized to plasma exposure, atenolol, sotalol and enalaprilat exhibited comparable mean arterial pressure, heart rate, and cardiac contractility responses regardless of formulation. While the antihypertensive effect of nifedipine was evident with both DPP and PEG-400 formulations, pressor effects from PEG-400 confounded interpretation of the magnitude of nifedipine's response. Plasma concentrations of atenolol and enalaprilat were greater in D5W formulation whereas sotalol exposures were greater when using DPP as a vehicle. These results demonstrate the utility of DPP as an intravenous vehicle for formulating poorly soluble compounds in early preclinical screening for cardiovascular safety studies.
authors.affiliation: Research and Development, AbbVie, Inc, 1 North Waukegan Rd, North Chicago, IL 60064, United States. Electronic address: Patricia.banfor@abbvie.com.
authors.forename: Patricia N
authors.initials: PN
authors.lastname: Banfor
authors.affiliation: Research and Development, AbbVie, Inc, 1 North Waukegan Rd, North Chicago, IL 60064, United States.
authors.forename: Gary A
authors.initials: GA
authors.lastname: Gintant
authors.affiliation: Research and Development, AbbVie, Inc, 1 North Waukegan Rd, North Chicago, IL 60064, United States.
authors.forename: John M
authors.initials: JM
authors.lastname: Lipari
authors.affiliation: Research and Development, AbbVie, Inc, 1 North Waukegan Rd, North Chicago, IL 60064, United States.
authors.forename: Philip D
authors.initials: PD
authors.lastname: Zocharski
mesh_terms: D000081:Acetamides / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity; D000818:Animals; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D055808:Drug Discovery / Q000379:methods; D004353:Drug Evaluation, Preclinical; D005079:Excipients / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D006439:Hemodynamics / Q000187:drug effects*; D007262:Infusions, Intravenous; D007928:Lethal Dose 50; D008297:Male; D008955:Models, Cardiovascular; D004364:Pharmaceutical Preparations / Q000008:administration & dosage* / Q000097:blood / Q000737:chemistry; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity; D019946:Propylene Glycol / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity; D017207:Rats, Sprague-Dawley; D054852:Small Molecule Libraries / Q000008:administration & dosage* / Q000009:adverse effects / Q000737:chemistry / Q000493:pharmacokinetics; D012995:Solubility
chemical_list: D000081:Acetamides; D004337:Drug Carriers; D005079:Excipients; D004364:Pharmaceutical Preparations; D054852:Small Molecule Libraries; D011092:Polyethylene Glycols; D019946:Propylene Glycol; C013959:dimethylacetamide
keywords: Blood pressure; Cardiac contractility; Cardiovascular screening; Formulation; Heart rate; Methods; Pharmacokinetic; Safety pharmacology; Solubility; Vehicle