title: Genotypic characterization of drug resistant Mycobacterium tuberculosis in Quebec, 2002-2012.
abstract: The increasing emergence of drug-resistant tuberculosis presents a threat to the effective control of tuberculosis (TB). Rapid detection of drug-resistance is more important than ever to address this scourge. The purpose of this study was to genotypically characterize the first-line antitubercular drug-resistant isolates collected over 11 years in Quebec.
  
  
  
  The main mutations found in our resistant strains collection (n = 225) include: the S315T substitution in katG (50.2 %), the -15 C/T mutation in the inhA promoter (29 %); the S531L substitution in rpoB (43 %); the deletion 8 bp 446 / + R140S in pncA (72.9 %); the M306I (35.7 %) and M306V (21.4 %) substitutions in embB. Ten of the mutations in katG and 4 mutations identified in pncA were previously undescribed.
  
  
  
  Screening of mutations conferring resistance to first-line antituberculous drugs using DNA-sequencing approach seems to be feasible and would drastically shorten the time to determine the resistance profile compared to the proportion method.
authors.affiliation: McGill University, Montreal, Quebec, Canada.|Present address: Public Health Ontario Laboratory, Toronto, Ontario, M5G 1 M1, Canada.
authors.forename: Joanna
authors.initials: J
authors.lastname: Spinato
authors.affiliation: Laboratoire de santé publique du Quebec, 20045 chemin Sainte-Marie, Sainte-Anne de Bellevue, Quebec, H9X 3R5, Canada.
authors.forename: Élyse
authors.initials: É
authors.lastname: Boivin
authors.affiliation: Laboratoire de santé publique du Quebec, 20045 chemin Sainte-Marie, Sainte-Anne de Bellevue, Quebec, H9X 3R5, Canada.
authors.forename: Émilie
authors.initials: É
authors.lastname: Bélanger-Trudelle
authors.affiliation: Laboratoire de santé publique du Quebec, 20045 chemin Sainte-Marie, Sainte-Anne de Bellevue, Quebec, H9X 3R5, Canada.
authors.forename: Huguette
authors.initials: H
authors.lastname: Fauchon
authors.affiliation: Université de Montreal, Montreal, Quebec, Canada.
authors.forename: Cécile
authors.initials: C
authors.lastname: Tremblay
authors.affiliation: Laboratoire de santé publique du Quebec, 20045 chemin Sainte-Marie, Sainte-Anne de Bellevue, Quebec, H9X 3R5, Canada. hafid.soualhine@inspq.qc.ca.
authors.forename: Hafid
authors.identifier: 0000-0002-5526-6741
authors.initials: H
authors.lastname: Soualhine
mesh_terms: D000581:Amidohydrolases / Q000235:genetics; D000995:Antitubercular Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D002374:Catalase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D010088:Oxidoreductases / Q000235:genetics; D010430:Pentosyltransferases / Q000235:genetics; D011792:Quebec; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D014376:Tuberculosis / Q000382:microbiology; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C505805:rpoB protein, Mycobacterium tuberculosis; D010088:Oxidoreductases; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; C085257:InhA protein, Mycobacterium; C540269:EmbB protein, Mycobacterium tuberculosis; D010430:Pentosyltransferases; D012321:DNA-Directed RNA Polymerases; D000581:Amidohydrolases; C487496:PncA protein, Mycobacterium tuberculosis
keywords: Drug-resistance; M. tuberculosis; embB; inhA; katG; pncA; rpoB