title: Enhanced accumulation of phytosterols and phenolic compounds in cyclodextrin-elicited cell suspension culture of Daucus carota.
abstract: In this work, suspension-cultured cells of Daucus carota were used to evaluate the effect of β-cyclodextrins on the production of isoprenoid and phenolic compounds. The results showed that the phytosterols and phenolic compounds were accumulated in the extracellular medium (15100μgL(-1) and 477.46μgL(-1), respectively) in the presence of cyclodextrins. Unlike the phytosterol and phenolic compound content, β-carotene (1138.03μgL(-1)), lutein (25949.54μgL(-1)) and α-tocopherol (8063.82μgL(-1)) chlorophyll a (1625.13μgL(-1)) and b (9.958 (9958.33μgL(-1)) were mainly accumulated inside the cells. Therefore, cyclodextrins were able to induce the cytosolic mevalonate pathway, increasing the biosynthesis of phytosterols and phenolic compounds, and accumulate them outside the cells. However, in the absence of these cyclic oligosaccharidic elicitors, carrot cells mainly accumulated carotenoids through the methylerythritol 4-phosphate pathway. Therefore, the use of cyclodextrins would allow the extracellular accumulation of both phytosterols and phenolic compounds by diverting the carbon flux towards the cytosolic mevalonate/phenylpropanoid pathway.
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, E-30100 Murcia, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Miras-Moreno
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, E-30100 Murcia, Spain. Electronic address: lorena.almagro@um.es.
authors.forename: Lorena
authors.initials: L
authors.lastname: Almagro
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, E-30100 Murcia, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Pedreño
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, E-30100 Murcia, Spain.
authors.forename: Ana Belén
authors.initials: AB
authors.lastname: Sabater-Jara
mesh_terms: D018929:Cell Culture Techniques; D018552:Daucus carota / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D010840:Phytosterols / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism*; D047392:beta-Cyclodextrins / Q000378:metabolism*
chemical_list: D010636:Phenols; D010840:Phytosterols; D013729:Terpenes; D047392:beta-Cyclodextrins
keywords: Carotenoids; Cyclodextrins; Green carrot suspension cultured cells; Phenolic compounds; Phytosterols; α-Tocopherol