title: Blood culture bottles are superior to conventional media for vitreous culture.
abstract: BACKGROUND
  To compare blood culture bottles and conventional media for the vitreous culture in patients with clinically suspected infectious endophthalmitis.
  
  
  METHODS
  Retrospective comparative study at KKU Eye Center, Khon Kaen University.
  
  
  METHODS
  There were 342 patients with clinically suspected infectious endophthalmitis participated in the study.
  
  
  METHODS
  The vitreous specimens were inoculated in both blood culture bottles and on conventional culture media (blood agar, MacConkey agar, chocolate agar, Sabouraud dextrose agar and thioglycolate broth).
  
  
  METHODS
  The number of positive culture yields in both blood culture bottles and conventional media.
  
  
  RESULTS
  Positive culture yields in both methods were found in 151 eyes (49.5%). There were 136 of 151 eyes (90.1%) with positive culture in blood culture bottles, whereas 99 of 151 eyes (65.6%) yielded positive cultures in conventional media. These findings were different with a statistical significance (P < 0.00001) and an odds ratio of 3.47 (95% confidence interval 1.92, 6.63). A combination of blood culture bottles and conventional media improved the yield.
  
  
  CONCLUSIONS
  Blood culture bottles are superior to conventional media for vitreous culture in clinically suspected infectious endophthalmitis. Vitreous culture using blood culture bottles should be recommended as the primary method for microbiological diagnosis. A combination of both methods further improves the positive culture yield.
authors.affiliation: KKU Eye Center, Department of Ophthalmology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Patsuda
authors.initials: P
authors.lastname: Thariya
authors.affiliation: KKU Eye Center, Department of Ophthalmology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Yosanan
authors.initials: Y
authors.lastname: Yospaiboon
authors.affiliation: KKU Eye Center, Department of Ophthalmology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Suthasinee
authors.initials: S
authors.lastname: Sinawat
authors.affiliation: KKU Eye Center, Department of Ophthalmology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Thuss
authors.initials: T
authors.lastname: Sanguansak
authors.affiliation: KKU Eye Center, Department of Ophthalmology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Chavakij
authors.initials: C
authors.lastname: Bhoomibunchoo
authors.affiliation: KKU Eye Center, Department of Ophthalmology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Wipada
authors.initials: W
authors.lastname: Laovirojjanakul
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D000071997:Blood Culture / Q000295:instrumentation*; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D009877:Endophthalmitis / Q000382:microbiology*; D015818:Eye Infections, Bacterial / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D014821:Vitrectomy; D014822:Vitreous Body / Q000382:microbiology*
chemical_list: D003470:Culture Media
keywords: blood culture bottle; conventional medium; infectious endophthalmitis; vitreous culture