title: Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3.
abstract: Bacteriophages express proteins that inactivate the CRISPR-Cas bacterial immune system. Here we report the crystal structure of the anti-CRISPR protein AcrF3 in complex with Pseudomonas aeruginosa Cas3 (PaCas3). AcrF3 forms a homodimer that locks PaCas3 in an ADP-bound form, blocks the entrance of the DNA-binding tunnel in the helicase domain, and masks the linker region and C-terminal domain of PaCas3, thereby preventing recruitment by Cascade and inhibiting the type I-F CRISPR-Cas system.
authors.affiliation: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Center for Protein Science Shanghai, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Deqiang
authors.initials: D
authors.lastname: Yao
authors.affiliation: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
authors.forename: Jin-Gen
authors.initials: JG
authors.lastname: Xu
authors.affiliation: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
authors.forename: A-Rong
authors.initials: AR
authors.lastname: Li
authors.affiliation: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
authors.forename: Jianpo
authors.initials: J
authors.lastname: Xu
authors.affiliation: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
authors.forename: Panhan
authors.initials: P
authors.lastname: Fu
authors.affiliation: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
authors.forename: Yongqun
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001435:Bacteriophages / Q000502:physiology*; D064130:CRISPR-Associated Proteins / Q000737:chemistry*; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D000072756:Protein Conformation, alpha-Helical; D054730:Protein Interaction Domains and Motifs; D020836:Protein Structure, Quaternary; D011550:Pseudomonas aeruginosa / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D064130:CRISPR-Associated Proteins; D014764:Viral Proteins