title: Influence of high-molecular-weight glutenin subunit composition at Glu-A1 and Glu-D1 loci on secondary and micro structures of gluten in wheat (Triticum aestivum L.).
abstract: As one of critical gluten proteins, high-molecular-weight glutenin subunits (HMW-GS) mainly affect the rheological behaviour of wheat dough. The influence of HMW-GS variations at the Glu-A1 and Glu-D1 loci on both secondary and micro structures of gluten and rheological properties of wheat dough was investigated in this study. Results showed that the Amide I bands of the three near-isogenic lines (NILs) shifted slightly, but the secondary structures differed significantly. The micro structure of gluten in NIL 4 (Ax null) showed bigger apertures and less connection, compared to that in Xinong 1330 (Ax1). The micro structure of gluten in NIL 5 (Dx5+Dy10) showed more compact than that in Xinong 1330 (Dx2+Dy12). Correlation analysis demonstrated that the content of β-sheets and disulfide bonds in gluten has a significant relationship with dough properties. The secondary structures of native gluten are suggested to be used as predictors of wheat quality.
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China. Electronic address: xuejun@nwsuaf.edu.cn.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China. Electronic address: liutianhong@nwsuaf.edu.cn.
authors.forename: Tianhong
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China. Electronic address: 1639524496@qq.com.
authors.forename: Lijun
authors.initials: L
authors.lastname: Song
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China. Electronic address: 1251844437@qq.com.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China. Electronic address: liliqunbangong@qq.com.
authors.forename: Liqun
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China. Electronic address: bestgaoxin@nwsuaf.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Gao
mesh_terms: D056426:Genetic Loci; D005903:Gliadin / Q000737:chemistry / Q000235:genetics; D005983:Glutens / Q000737:chemistry / Q000235:genetics*; D008970:Molecular Weight; D000072757:Protein Conformation, beta-Strand; D017433:Protein Structure, Secondary; D019265:Spectroscopy, Near-Infrared; D014908:Triticum / Q000737:chemistry / Q000235:genetics*
chemical_list: D005983:Glutens; D005903:Gliadin; C037302:glutenin
keywords: Dough rheological behaviour; Glu-A1 and Glu-D1; Micro structure; Secondary structure of gluten; Wheat glutenin subunits