title: Increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae by disruption of the genes encoding cell wall α-1,3-glucan synthase.
abstract: Under liquid culture conditions, the hyphae of filamentous fungi aggregate to form pellets, which reduces cell density and fermentation productivity. Previously, we found that loss of α-1,3-glucan in the cell wall of the fungus Aspergillus nidulans increased hyphal dispersion. Therefore, here we constructed a mutant of the industrial fungus A. oryzae in which the three genes encoding α-1,3-glucan synthase were disrupted (tripleΔ). Although the hyphae of the tripleΔ mutant were not fully dispersed, the mutant strain did form smaller pellets than the wild-type strain. We next examined enzyme productivity under liquid culture conditions by transforming the cutinase-encoding gene cutL1 into A. oryzae wild-type and the tripleΔ mutant (i.e. wild-type-cutL1, tripleΔ-cutL1). A. oryzae tripleΔ-cutL1 formed smaller hyphal pellets and showed both greater biomass and increased CutL1 productivity compared with wild-type-cutL1, which might be attributable to a decrease in the number of tripleΔ-cutL1 cells under anaerobic conditions.
authors.affiliation: a Laboratory of Applied Microbiology, Department of Microbial Biotechnology , Graduate School of Agricultural Science, Tohoku University , Sendai , Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Miyazawa
authors.affiliation: b Microbial Genomics Laboratory , New Industry Creation Hatchery Center, Tohoku University , Sendai , Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Yoshimi
authors.affiliation: c Laboratory of Bioindustrial Genomics, Department of Bioindustrial Informatics and Genomics , Graduate School of Agricultural Science, Tohoku University , Sendai , Japan.
authors.forename: Silai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: d Genome Biotechnology Laboratory , Kanazawa Institute of Technology , Hakusan , Japan.
authors.forename: Motoaki
authors.initials: M
authors.lastname: Sano
authors.affiliation: a Laboratory of Applied Microbiology, Department of Microbial Biotechnology , Graduate School of Agricultural Science, Tohoku University , Sendai , Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Nakayama
authors.affiliation: c Laboratory of Bioindustrial Genomics, Department of Bioindustrial Informatics and Genomics , Graduate School of Agricultural Science, Tohoku University , Sendai , Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Gomi
authors.affiliation: a Laboratory of Applied Microbiology, Department of Microbial Biotechnology , Graduate School of Agricultural Science, Tohoku University , Sendai , Japan.|b Microbial Genomics Laboratory , New Industry Creation Hatchery Center, Tohoku University , Sendai , Japan.
authors.forename: Keietsu
authors.initials: K
authors.lastname: Abe
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics; D018533:Biomass; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000235:genetics*; D025301:Hyphae / Q000235:genetics / Q000254:growth & development; D050505:Mutant Proteins / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation
chemical_list: D005936:Glucans; D050505:Mutant Proteins; C045788:alpha-1,3-glucan; C036655:1,3-alpha-D-glucan synthase; D005964:Glucosyltransferases
keywords: Aspergillus; cell wall; enzyme; production; α-1,3-glucan