title: Two new bioactive iridoids from Rothmannia wittii.
abstract: The first reported study of the isolation and identification of compounds from the bark and fruit of Rothmannia wittii yielded two new iridoids, 6β-hydroxy-10-O-acetylgenipin (1) and 10-O-acetylmacrophyllide (2) together with six known iridoids; 6β-hydroxygenipin (3), genipin (4), garjasmine (5), cerbinal (6), and mixture of β-gardiol (7) and α-gardiol (8); benzoic acid (9); vanillic acid (10); and stigmasterol (11). Their structures were elucidated by spectroscopic methods. Iridoid 1 showed antimycobacterial activity against Mycobacterium tuberculosis with a MIC value of 12.50μg/mL. Iridoid 2 showed cytotoxicity against the NCI-H187 cancer cell line with an IC50 value of 6.82μg/mL. In addition, 2 and 5 exhibited weak cytotoxic activity against KB and MCF-7 cell lines, while 4 was active against the NCI-H187 cancer cell line.
authors.affiliation: Natural Products Research Unit, Department of Chemistry, Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: nattapong-c@hotmail.com.
authors.forename: Nattapong
authors.initials: N
authors.lastname: Chaipukdee
authors.affiliation: Natural Products Research Unit, Department of Chemistry, Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: kwanjai@kku.ac.th.
authors.forename: Kwanjai
authors.initials: K
authors.lastname: Kanokmedhakul
authors.affiliation: Natural Products Research Unit, Department of Chemistry, Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: somdej@kku.ac.th.
authors.forename: Somdej
authors.initials: S
authors.lastname: Kanokmedhakul
authors.affiliation: Natural Products Research Unit, Department of Chemistry, Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: ratsami@kku.ac.th.
authors.forename: Ratsami
authors.initials: R
authors.lastname: Lekphrom
authors.affiliation: School of Chemistry, University of Wollongong, Wollongong, New South Wales 2522, Australia. Electronic address: spyne@uow.edu.au.
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Pyne
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D005638:Fruit / Q000737:chemistry; D006801:Humans; D039823:Iridoids / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D024301:Plant Bark / Q000737:chemistry; D019666:Rubiaceae / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic; D039823:Iridoids
keywords: Cerbinal; Garjasmine; Genipin; Iridoid; Macrophyllide; Rothmannia wittii