title: RCAN1 deficiency protects against Salmonella intestinal infection by modulating JNK activation.
abstract: RCAN1 (regulator of calcineurin 1) has been shown to be involved in various physiological and pathological processes. However, the biological implications of RCAN1 during gastrointestinal tract infection remain unclear. In this study, we tried to determine the role of RCAN1 in acute Salmonella infectious colitis.
  
  
  
  Wild type and RCAN1-deficient mice or macrophages were used to characterize the impacts of RCAN1 on intestinal inflammation, inflammatory cytokines production, animal survival, and pathogen clearance following Salmonella challenge.
  
  
  
  Histologic and quantitative assessments showed increased inflammation and elevated proinflammatory cytokines production in RCAN1-deficient mice. The aberrant inflammatory response was recapitulated in primary bone marrow-derived macrophages. In addition, we reveal a novel regulatory role for RCAN1 in the proinflammatory JNK signaling both in vitro and in vivo. Further analysis showed that the increased inflammation in RCAN1-deficient mice contributed to pathogen clearance and host survival.
  
  
  
  The present study demonstrates that RCAN1 deficiency protects against Salmonella intestinal infection by enhancing proinflammatory JNK signaling.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun 130062, China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Lei
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun 130062, China.
authors.forename: Gui-Qiu
authors.initials: GQ
authors.lastname: Hu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun 130062, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun 130062, China.
authors.forename: Shui-Xing
authors.initials: SX
authors.lastname: Yu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun 130062, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Qi
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun 130062, China.
authors.forename: Chong-Tao
authors.initials: CT
authors.lastname: Du
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun 130062, China.
authors.forename: Jing-Min
authors.initials: JM
authors.lastname: Gu
authors.affiliation: Department of Pediatrics, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: Tong-Jun
authors.initials: TJ
authors.lastname: Lin
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun 130062, China. Electronic address: youngjune@jlu.edu.cn.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Yang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002135:Calcium-Binding Proteins; D003092:Colitis / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000172:deficiency / Q000276:immunology*; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009124:Muscle Proteins / Q000172:deficiency / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; C511512:DSCR1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D009124:Muscle Proteins; D048670:MAP Kinase Kinase 4
keywords: Colitis; Inflammation; JNK activation; RCAN1; Salmonella