title: Genetic variations of DICKKOPF family genes might not be associated with gastric cancer susceptibility.
abstract: BACKGROUND
  Recent studies have implicated that members of the DICKKOPF (DKK) were causally involved in large number of human cancers. This study was designed to investigate the relationship between the genetic variations of DKK family genes and the risk of gastric cancer (GC).
  
  
  METHODS
  Six SNPs (single nucleotide polymorphisms) of DKK family genes, including rs2241529 in DKK1, rs3733635, rs17037102 and rs419764 in DKK2, rs3206824 in DKK3 and rs2073664 in DKK4, were selected and genotyped by restriction fragment length polymorphism (RFLP) and TaqMan SNP genotyping methods in 409 GC cases and 554 cancer-free controls in the Han population in eastern China.
  
  
  RESULTS
  None of the six SNPs achieved significant association with the overall GC risk and stratified analysis by age, gender, smoking status, drinking status, tumor location and pathological classification confirmed these non-significant associations.
  
  
  CONCLUSIONS
  Our study indicated that the studied six SNPs of DKKs would not be the risk factors for GC in this Han Chinese population. Studies of larger population for different ethnicities will be needed to warrant our findings.
authors.affiliation: Department of Nutrition and Food Hygiene, Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, 818 East Tianyuan Rd, Nanjing, China.|Discipline of Chinese and Western Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wu
authors.affiliation: Discipline of Chinese and Western Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Discipline of Chinese and Western Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhan
authors.affiliation: Department of Digestive Tumor Surgery, Jiangsu Province Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Qinhong
authors.initials: Q
authors.lastname: Cao
authors.affiliation: Department of Nutrition and Food Hygiene, Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, 818 East Tianyuan Rd, Nanjing, China. lz-ny@njmu.edu.cn.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D044466:Asian People / Q000235:genetics; D018925:Chemokines; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D060005:Genotyping Techniques; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018925:Chemokines; C501126:DKK1 protein, human; C498622:DKK2 protein, human; C498624:DKK3 protein, human; C442333:DKK4 protein, human; D036341:Intercellular Signaling Peptides and Proteins
keywords: Epidemiology; Single nucleotide polymorphism; Stomach neoplasms; Wnt signaling pathway