title: Local administration of siRNA through Microneedle: Optimization, Bio-distribution, Tumor Suppression and Toxicity.
abstract: Although RNA interference may become a novel therapeutic approach for cancer treatment, target-site accumulation of siRNA to achieve therapeutic dosage will be a major problem. Microneedle represents a better way to deliver siRNAs and we have evaluated for the first time the capability of a silicon microneedle array for delivery of Gapdh siRNA to the skin in vivo and the results showed that the microneedle arrays could effectively deliver siRNA to relevant regions of the skin noninvasively. For the further study in this field, we evaluated the efficacy of the injectable microneedle device for local delivery of siRNA to the mouse xenograft. The results presented here indicate that local administration of siRNA through injectable microneedle could effectively deliver siRNA into the tumor region, and inhibit tumor progression without major adverse effects.
authors.affiliation: Department of Obstetrics &Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.|Shenzhen Research Institute, The Chinese University of Hong Kong, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tang
authors.affiliation: Department of Obstetrics &Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.|Shenzhen Research Institute, The Chinese University of Hong Kong, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Obstetrics &Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.|Shenzhen Research Institute, The Chinese University of Hong Kong, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Obstetrics &Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Microelectronics, Tsinghua University, Beijing, China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Yue
authors.affiliation: Department of Obstetrics &Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.|Shenzhen Research Institute, The Chinese University of Hong Kong, China.
authors.forename: Kwong Wai
authors.initials: KW
authors.lastname: Choy
authors.affiliation: Department of Obstetrics &Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.|Shenzhen Research Institute, The Chinese University of Hong Kong, China.|Li Ka Shing Institute of Health Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.|School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
authors.forename: Chi Chiu
authors.initials: CC
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Du
authors.affiliation: Department of Obstetrics &Gynaecology, Guangzhou panyu Central hospital, Guangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Dongguan Third People's Hospital, Affiliated Dongguan Shilong People's Hospital of Southern Medical University, Dongguan, China.
authors.forename: Linxiao
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Obstetrics &Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
authors.forename: Tony Kwok Hung
authors.initials: TK
authors.lastname: Chung
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000378:metabolism; D016503:Drug Delivery Systems; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D007267:Injections; D008807:Mice, Inbred BALB C; D008845:Microinjections; D009339:Needles; D009369:Neoplasms / Q000473:pathology*; D009856:Oncogene Proteins, Viral; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000633:toxicity*; D012097:Repressor Proteins; D014018:Tissue Distribution; D023041:Xenograft Model Antitumor Assays
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D002784:Cholesterol