title: Large-scale development of SSR markers in tobacco and construction of a linkage map in flue-cured tobacco.
abstract: Tobacco (Nicotiana tabacum L.), particularly flue-cured tobacco, is one of the most economically important nonfood crops and is also an important model system in plant biotechnology. Despite its importance, only limited molecular marker resources are available for genome analysis, genetic mapping, and breeding. Simple sequence repeats (SSR) are one of the most widely-used molecular markers, having significant advantages including that they are generally co-dominant, easy to use, abundant in eukaryotic organisms, and produce highly reproducible results. In this study, based on the genome sequence data of flue-cured tobacco (K326), we developed a total of 13,645 mostly novel SSR markers, which were working in a set of eighteen tobacco varieties of four different types. A mapping population of 213 backcross (BC1) individuals, which were derived from an intra-type cross between two flue-cured tobacco varieties, Y3 and K326, was selected for mapping. Based on the newly developed SSR markers as well as published SSR markers, we constructed a genetic map consisting of 626 SSR loci distributed across 24 linkage groups and covering a total length of 1120.45 cM with an average distance of 1.79 cM between adjacent markers, which is the highest density map of flue-cured tobacco till date.
authors.affiliation: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences , Kunming 650021 , China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Tong
authors.affiliation: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences , Kunming 650021 , China.
authors.forename: Bingguang
authors.initials: B
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences , Kunming 650021 , China.
authors.forename: Fangchan
authors.initials: F
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences , Kunming 650021 , China.
authors.forename: Dunhuang
authors.initials: D
authors.lastname: Fang
authors.affiliation: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences , Kunming 650021 , China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences , Kunming 650021 , China.
authors.forename: Xingfu
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences , Kunming 650021 , China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences , Kunming 650021 , China.
authors.forename: Jiankang
authors.initials: J
authors.lastname: Yang
authors.affiliation: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences , Kunming 650021 , China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Li
keywords: flue-cured tobacco (Nicotiana tabacum L.); genetic map; molecular marker; simple sequence repeats (SSR)