title: Knockdown of Zinc Transporter ZIP5 by RNA Interference Inhibits Esophageal Cancer Growth In Vivo.
abstract: We recently found that SLC39A5 (ZIP5), a zinc transporter, is overexpressed in esophageal cancer. Downregulation of ZIP5 inhibited the proliferation, migration, and invasion of the esophageal cancer cell line KYSE170 in vitro. In this study, we found that downregulation of SLC39A5 (ZIP5) by interference resulted in a significant reduction in esophageal cancer tumor volume and weight in vivo. COX2 (cyclooxygenase 2) expression was decreased and E-cadherin expression was increased in the KYSE170K xenografts, which was caused by the downregulation of ZIP5. However, we did not find that the downregulation of ZIP5 caused a change in the relative expressions of cyclin D1, VEGF (vascular endothelial growth factor), MMP9 (matrix metalloprotein 9), and Bcl-2 (B-cell lymphoma/leukmia-2) mRNA or an alteration in the average level of zinc in the peripheral blood and xenografts in vivo. Collectively, these findings indicate that knocking down ZIP5 by small interfering RNA (siRNA) might be a novel treatment strategy for esophageal cancer with ZIP5 overexpression.
authors.affiliation: Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.forename: Jianghui
authors.initials: J
authors.lastname: Liu
authors.forename: Liqun
authors.initials: L
authors.lastname: Wang
authors.forename: Yutong
authors.initials: Y
authors.lastname: He
mesh_terms: D000818:Animals; D015415:Biomarkers; D027682:Cation Transport Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors / Q000235:genetics; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D034622:RNA Interference; D014161:Transduction, Genetic; D047368:Tumor Burden; D015032:Zinc / Q000097:blood / Q000378:metabolism
chemical_list: D015415:Biomarkers; D027682:Cation Transport Proteins; C457225:SLC30A5 protein, human; D015032:Zinc