title: Arabidopsis NAP1 Regulates the Formation of Autophagosomes.
abstract: The SCAR/WAVE complex is required for ARP2/3-mediated actin nucleation, and these complexes are highly conserved in plants and animals [1, 2]. Proteins from the SCAR/WAVE complex have been found to be membrane associated in plants [3]. Using fluorescent protein fusions, we have found that NAP1 [4], a component of the SCAR/WAVE complex, locates to vesicles or puncta that appear upon applied pressure. These NAP1 vesicles can be endoplasmic reticulum (ER)-associated, can co-align with the cytoskeleton, and fuse to each other homotypically. More interestingly, the majority co-localizes with the autophagosome marker ATG8, and anti-NAP1 identifies autophagosomes in immuno-TEM. Macroautophagy (hereafter referred to as autophagy) is enhanced under certain stress conditions such as nitrogen starvation and salt stress. We show that fewer autophagosomes are generated in the NAP1 knockout mutant during starvation stress. The nap1 mutant (and KO mutants of other components of the SCAR/WAVE and ARP2/3 complexes) is more susceptible to nitrogen starvation and is less salt tolerant, indicating defective autophagy. In conclusion, our data show that NAP1 has another function in plant cells, and that is as a regulator of autophagy.
authors.affiliation: School of Biological and Biomedical Sciences, Durham University, Durham DH1 3LE, UK.
authors.forename: Pengwei
authors.initials: P
authors.lastname: Wang
authors.affiliation: School of Biological and Biomedical Sciences, Durham University, Durham DH1 3LE, UK.
authors.forename: Christine
authors.initials: C
authors.lastname: Richardson
authors.affiliation: Department of Biological and Medical Sciences, Oxford Brookes University, Oxford OX3 0BP, UK.
authors.forename: Chris
authors.initials: C
authors.lastname: Hawes
authors.affiliation: School of Biological and Biomedical Sciences, Durham University, Durham DH1 3LE, UK. Electronic address: p.j.hussey@durham.ac.uk.
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Hussey
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D000071182:Autophagosomes / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D000251:Adenosine Triphosphatases; C498859:NAP1 protein, Arabidopsis