title: Reprogramming bladder cancer cells for studying cancer initiation and progression.
abstract: The induced pluripotent stem cell (iPSC) technology is the forced expression of specific transcription factors in somatic cells resulting in transformation into self-renewing, pluripotent cells which possess the ability to differentiate into any type of cells in the human body. While malignant cells could also be reprogrammed into iPSC-like cells with lower efficiency due to the genetic and epigenetic barriers in cancer cells, only a limited number of cancer cell types could be successfully reprogrammed until today. In the present study, we aimed at reprogramming two bladder cancer cell lines HTB-9 and T24 using a non-integrating Sendai virus (SeV) system. We have generated six sub-clones using distinct combinations of four factors-OCT4, SOX2, KLF4 and c-MYC-in two bladder cancer cell lines. Only a single sub-clone, T24 transduced with 4Fs, gave rise to iPSC-like cells. Bladder cancer cell-derived T24 4F cells represent unique features of pluripotent cells such as epithelial-like morphology, colony-forming ability, expression of pluripotency-associated markers and bearing the ability to differentiate in vitro. This is the first study focusing on the reprogramming susceptibility of two different bladder cancer cell lines to nuclear reprogramming. Further molecular characterisation of T24 4F cells could provide a better insight for biomarker research in bladder carcinogenesis and could offer a valuable tool for the development of novel therapeutic approaches in bladder carcinoma.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Erciyes University, Melikgazi, 38039, Kayseri, Turkey. banu.iskender@yahoo.com.|Betul-Ziya Eren Genome and Stem Cell Centre, Erciyes University, Melikgazi, 38039, Kayseri, Turkey. banu.iskender@yahoo.com.
authors.forename: Banu
authors.initials: B
authors.lastname: Iskender
authors.affiliation: Betul-Ziya Eren Genome and Stem Cell Centre, Erciyes University, Melikgazi, 38039, Kayseri, Turkey.|Department of Medical Biochemistry, Faculty of Medicine, Erciyes University, Melikgazi, 38039, Kayseri, Turkey.
authors.forename: Kenan
authors.initials: K
authors.lastname: Izgi
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Erciyes University, Melikgazi, 38039, Kayseri, Turkey.|Betul-Ziya Eren Genome and Stem Cell Centre, Erciyes University, Melikgazi, 38039, Kayseri, Turkey.
authors.forename: Halit
authors.initials: H
authors.lastname: Canatan
mesh_terms: D015415:Biomarkers; D045744:Cell Line, Tumor; D054338:Cell Transdifferentiation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000209:etiology* / Q000473:pathology*
chemical_list: D015415:Biomarkers; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D050814:Octamer Transcription Factor-3; D016271:Proto-Oncogene Proteins c-myc; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors
keywords: Bladder cancer; HTB-9; Induced pluripotent stem cells; Pluripotency; Reprogramming; T24