title: Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A.
abstract: A full-length cDNA infectious clone, pKS15-01-Clone, was constructed from an emerging Senecavirus A (SVA; strain KS15-01). To explore the potential use as a viral backbone for expressing marker genes, the enhanced green fluorescent protein (EGFP)-tagged reporter virus (vKS15-01-EGFP) was generated using reverse genetics. Compared to the parental virus, the pKS15-01-Clone derived virus (vKS15-01-Clone) replicated efficiently in vitro and in vivo, and induced similar levels of neutralizing antibody and cytokine responses in infected animals. In contrast, the vKS15-01-EGFP virus showed impaired growth ability and induced lower level of immune response in infected animals. Lesions on the dorsal snout and coronary bands were observed in all pigs infected by parental virus KS15-01, but not in pigs infected with vKS15-01-Clone or vKS15-01-EGFP viruses. These results demonstrated that the infectious clone and EGFP reporter virus could be used as important tools in further elucidating the SVA pathogenesis and development of control measures.
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States.
authors.forename: Zhenhai
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States; Kansas State Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States.
authors.forename: Fangfeng
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Shang
authors.affiliation: Midwest Veterinary Services, Inc., Oakland, NE 68045, United States.
authors.forename: Robin
authors.initials: R
authors.lastname: Schroeder
authors.affiliation: Midwest Veterinary Services, Inc., Oakland, NE 68045, United States.
authors.forename: Kelly
authors.initials: K
authors.lastname: Lechtenberg
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States; Kansas State Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States.
authors.forename: Jamie
authors.initials: J
authors.lastname: Henningson
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States; Kansas State Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Hause
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States; Kansas State Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States.
authors.forename: Jianfa
authors.initials: J
authors.lastname: Bai
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States.
authors.forename: Raymond R R
authors.initials: RRR
authors.lastname: Rowland
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States; Kansas State Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Clavijo
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States; Kansas State Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States. Electronic address: yfang@vet.k-state.edu.
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D016207:Cytokines / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D016679:Genome, Viral; D007113:Immunity, Innate; D009154:Mutation; D010641:Phenotype; D010849:Picornaviridae / Q000235:genetics* / Q000276:immunology; D010850:Picornaviridae Infections / Q000662:veterinary; D012367:RNA, Viral; D011995:Recombination, Genetic; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D018076:DNA, Complementary; D012367:RNA, Viral
keywords: EGFP reporter virus; Infectious clone; Picornavirus infection; Reverse genetics; SVA pathogenesis; Senecavirus A; Swine; Vesicular disease; Vesicular lesion