title: The role of budABC on 1,3-propanediol and 2,3-butanediol production from glycerol in Klebsiella pneumoniae CICIM B0057.
abstract: 1,3-propanediol (1,3-PD) is an important compound from which many others can be synthesized. 2,3-butanediol (BDO) is the key by-product in the biosynthesis of 1,3-PD from glycerol, but it impedes its downstream purification. In Klebsiella, the budA, budB and budC genes encode enzymes that are responsible for the synthesis of BDO. In this study, 3 individual antisense RNAs were designed to repress the expression and hence activity of BudA-C. Compared with the parent strains, the activities of BudB and BudC were reduced by 60.5% and 70.5%, respectively, and the mRNA level of budA was reduced by 70%. Decreased BudC activity had no effect on cell growth or carbon distribution. However, reduced BudA and BudB activity decreased the BDO concentration by 35% and led to a 10% increase in the yield of 1,3-PD. This result suggests the activities of BudA and BudB could be key factors in the production of BDO from glycerol in Klebsiella. This study provides a deeper understanding of the role of budABC in glycerol metabolism in Klebsiella.
authors.affiliation: a The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.
authors.forename: Xinyao
authors.initials: X
authors.lastname: Lu
authors.affiliation: a The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.
authors.forename: Guangjian
authors.initials: G
authors.lastname: Ji
authors.affiliation: a The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zong
authors.affiliation: a The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.|b The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi, Jiangsu , China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhuge
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002072:Butylene Glycols / Q000737:chemistry*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005990:Glycerol / Q000378:metabolism*; D007218:Industrial Microbiology; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000378:metabolism*; D011409:Propylene Glycols / Q000737:chemistry*; D016372:RNA, Antisense / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002072:Butylene Glycols; D011409:Propylene Glycols; D016372:RNA, Antisense; C026978:2,3-butylene glycol; C041787:1,3-propanediol; D005990:Glycerol
keywords: 1,3-propanediol; 2,3-butanediol; Klebsiella; antisense RNA; budABC