title: COPI is essential for Golgi cisternal maturation and dynamics.
abstract: Proteins synthesized in the endoplasmic reticulum (ER) are transported to the Golgi and then sorted to their destinations. For their passage through the Golgi, one widely accepted mechanism is cisternal maturation. Cisternal maturation is fulfilled by the retrograde transport of Golgi-resident proteins from later to earlier cisternae, and candidate carriers for this retrograde transport are coat protein complex I (COPI)-coated vesicles. We examined the COPI function in cisternal maturation directly by 4D observation of the transmembrane Golgi-resident proteins in living yeast cells. COPI temperature-sensitive mutants and induced degradation of COPI proteins were used to knockdown COPI function. For both methods, inactivation of COPI subunits Ret1 and Sec21 markedly impaired the transition from cis to medial and to trans cisternae. Furthermore, the movement of cisternae within the cytoplasm was severely restricted when COPI subunits were depleted. Our results demonstrate the essential roles of COPI proteins in retrograde trafficking of the Golgi-resident proteins and dynamics of the Golgi cisternae.
authors.affiliation: Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Ishii
authors.affiliation: Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan Laboratory of Molecular Cell Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Suda
authors.affiliation: Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan kkurokawa@riken.jp nakano@bs.s.u-tokyo.ac.jp.
authors.forename: Kazuo
authors.identifier: 0000-0003-3549-4795
authors.initials: K
authors.lastname: Kurokawa
authors.affiliation: Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan kkurokawa@riken.jp nakano@bs.s.u-tokyo.ac.jp.
authors.forename: Akihiko
authors.identifier: 0000-0003-3635-548X
authors.initials: A
authors.lastname: Nakano
mesh_terms: D020755:Coat Protein Complex I / Q000378:metabolism*; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D059748:Proteolysis / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
chemical_list: D020755:Coat Protein Complex I; D007210:Indoleacetic Acids; D008565:Membrane Proteins
keywords: COPI; Cisternal maturation; Golgi