title: The oviductal transcriptome is influenced by a local ovarian effect in the sow.
abstract: BACKGROUND
  Oviducts participate in fertilization and early embryo development, and they are influenced by systemic and local circulation. Local functional interplay between ovary, oviduct and uterus is important, as deduced from the previously observed differences in hormone concentrations, presence of sperm, or patterns of motility in the oviduct after unilateral ovariectomy (UO). However, the consequences of unilateral ovariectomy on the oviductal transcriptome remain unexplored. In this study, we have investigated the consequences of UO in a higher animal model as the pig.
  
  
  METHODS
  The influence of UO was analyzed on the number of ovulations on the contra ovary, which was increased, and on the ipsilateral oviductal transcriptome. Microarray analysis was performed and the results were validated by PCR. Differentially expressed genes (DEGs) with a fold change ≥ 2 and a false discovery rate of 10 % were analyzed by Ingenuity Pathway Analysis (IPA) to identify the main biofunctions affected by UO.
  
  
  RESULTS
  Data revealed two principal effects in the ipsilateral oviduct after UO: i) down-regulation of genes involved in the survival of sperm in the oviduct and early embryonic development, and ii) up-regulation of genes involved in others functions as protection against external agents and tumors.
  
  
  CONCLUSIONS
  Results showed that unilateral ovariectomy results in an increased number of ovulation points on the contra ovary and changes in the transcriptome of the ipsilateral oviduct with consequences on key biological process that could affect fertility output.
authors.affiliation: Department of Physiology, Veterinary Faculty, University of Murcia, Campus de Espinardo, 30100, Murcia, Spain.|International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), Murcia, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: López-Úbeda
authors.affiliation: Centro de Biotecnología Animal - SERIDA, Deva, Gijón, Asturias, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Muñoz
authors.affiliation: Department of Physiology, Veterinary Faculty, University of Murcia, Campus de Espinardo, 30100, Murcia, Spain.|International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), Murcia, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Vieira
authors.affiliation: Sidney Sussex College, Cambridge, UK.
authors.forename: Ronald H F
authors.initials: RH
authors.lastname: Hunter
authors.affiliation: Department of Physiology, Veterinary Faculty, University of Murcia, Campus de Espinardo, 30100, Murcia, Spain. pcoy@um.es.|International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), Murcia, Spain. pcoy@um.es.|IMIB-Arrixaca (Institute for Biomedical Research of Murcia), Murcia, Spain. pcoy@um.es.
authors.forename: Pilar
authors.initials: P
authors.lastname: Coy
authors.affiliation: Department of Physiology, Veterinary Faculty, University of Murcia, Campus de Espinardo, 30100, Murcia, Spain. scber@um.es.|International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), Murcia, Spain. scber@um.es.|IMIB-Arrixaca (Institute for Biomedical Research of Murcia), Murcia, Spain. scber@um.es.
authors.forename: Sebastian
authors.identifier: http://orcid.org/0000-0003-4190-6258
authors.initials: S
authors.lastname: Canovas
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D023421:Models, Animal; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology*; D010057:Oviducts / Q000502:physiology*; D010060:Ovulation / Q000235:genetics; D011247:Pregnancy; D013552:Swine; D046888:Tissue Array Analysis / Q000379:methods; D059467:Transcriptome; D014599:Uterus / Q000502:physiology
keywords: Microarray; Ovariectomy; Oviductal transcriptome; Pig