title: Comparative Analysis for the Presence of IgG Anti-Aquaporin-1 in Patients with NMO-Spectrum Disorders.
abstract: Detection of IgG anti-Aquaporin-4 (AQP4) in serum of patients with Neuromyelitis optica syndrome disorders (NMOSD) has improved diagnosis of these processes and differentiation from Multiple sclerosis (MS). Recent findings also claim that a subgroup of patients with NMOSD, serum negative for IgG-anti-AQP4, present antibodies anti-AQP1 instead. Explore the presence of IgG-anti-AQP1 using a previously developed cell-based assay (CBA) highly sensitive to IgG-anti-AQP4. Serum of 205 patients diagnosed as NMOSD (8), multiple sclerosis (94), optic neuritis (39), idiopathic myelitis (29), other idiopathic demyelinating disorders of the central nervous system (9), other neurological diseases (18) and healthy controls (8), were used in a CBA over fixed HEK cells transfected with hAQP1-EGFP or hM23-AQP4-EGFP, treated with Triton X-100 and untreated. ELISA was also performed. Analysis of serum with our CBA indicated absence of anti-AQP1 antibodies, whereas in cells pretreated with detergent, noisy signal made reliable detection impossible. ELISA showed positive results in few serums. The low number of NMOSD serums included in our study reduces its power to conclude the specificity of AQP1 antibodies as new biomarkers of NMOSD. Our study does not sustain detection of anti-AQP1 in serum of NMOSD patients but further experiments are expected.
authors.affiliation: Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Roc&#237;o/CSIC/Universidad de Sevilla, Seville 41013, Spain. ismael_sg84@hotmail.com.
authors.forename: Ismael
authors.initials: I
authors.lastname: Sánchez Gomar
authors.affiliation: Unidad de Gesti&#243;n Cl&#237;nica de Neurociencias, Servicio de Neurolog&#237;a del Hospital Universitario Virgen del Roc&#237;o, Seville 41013, Spain. mariadiazsanchez@hotmail.com.
authors.forename: María
authors.initials: M
authors.lastname: Díaz Sánchez
authors.affiliation: Unidad de Gesti&#243;n Cl&#237;nica de Neurociencias, Servicio de Neurolog&#237;a del Hospital Universitario Virgen del Roc&#237;o, Seville 41013, Spain. antonioj.ucles@gmail.com.
authors.forename: Antonio José
authors.initials: AJ
authors.lastname: Uclés Sánchez
authors.affiliation: Unidad de Gesti&#243;n Cl&#237;nica de Neurociencias, Servicio de Neurolog&#237;a del Hospital Universitario Virgen del Roc&#237;o, Seville 41013, Spain. jcasadoch@gmail.com.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Casado Chocán
authors.affiliation: Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Roc&#237;o/CSIC/Universidad de Sevilla, Seville 41013, Spain. neluqui@yahoo.es.
authors.forename: Nela
authors.initials: N
authors.lastname: Suárez-Luna
authors.affiliation: Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Roc&#237;o/CSIC/Universidad de Sevilla, Seville 41013, Spain. reporamirez@us.es.
authors.forename: Reposo
authors.initials: R
authors.lastname: Ramírez-Lorca
authors.affiliation: Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Roc&#237;o/CSIC/Universidad de Sevilla, Seville 41013, Spain. fvilladiego@us.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Villadiego
authors.affiliation: Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Roc&#237;o/CSIC/Universidad de Sevilla, Seville 41013, Spain. juanjo@us.es.|Centro de Investigaci&#243;n Biom&#233;dica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid 28029, Spain. juanjo@us.es.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Toledo-Aral
authors.affiliation: Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Roc&#237;o/CSIC/Universidad de Sevilla, Seville 41013, Spain. irusta@us.es.|Centro de Investigaci&#243;n Biom&#233;dica en Red sobre Enfermedades Respiratorias (CIBERES), Madrid 28029, Spain. irusta@us.es.
authors.forename: Miriam
authors.initials: M
authors.lastname: Echevarría
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000888:Antibodies, Anti-Idiotypic / Q000097:blood / Q000276:immunology*; D051398:Aquaporin 1 / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D005500:Follow-Up Studies; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009471:Neuromyelitis Optica / Q000097:blood / Q000276:immunology*; D011379:Prognosis; D055815:Young Adult
chemical_list: D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D015415:Biomarkers; D007074:Immunoglobulin G; C048844:anti-IgG; D051398:Aquaporin 1
keywords: AQP1; AQP4; HEK cells; NMO-IgG; neuromyelitis optica