title: Glutathione and glutathione S-transferase levels in patients with liver metastases of colorectal cancer and other hepatic disorders.
abstract: OBJECTIVE
  Glutathione and glutathione S-transferases (GST) are involved in cell defence against reactive oxygen species, which induces oxidative stress and are associated with different chronic diseases. The aim of the present study was to determine the differences in reduced glutathione (GSH) and GST levels in patients with different liver diseases.
  
  
  METHODS
  Overall, 114 patients were enrolled in this study: 58 patients with colorectal cancer (18 without and 40 with liver metastases), 27 with liver steatosis, 29 with alcoholic cirrhosis and a group of 40 healthy volunteers. The levels of GSH and GST in blood serum were evaluated by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's guidelines.
  
  
  RESULTS
  Significant differences in GSH and GST levels were observed in most of the groups compared to the healthy volunteers (GSH: 52.72 µg/mL, GST: 0.53 ng/mL): with hepatic steatosis (GSH: 17.04 µg/mL, p < 0.001; GST: 5.89 ng/mL, p < 0.001), alcoholic cirrhosis (GSH: 62.04 µg/mL, p < 0.003; GST: 0.94 ng/mL, p < 0.001) and liver metastases (GSH: 37.84 µg/mL, p < 0.001, GST: 1.25 ng/mL, p=0.747).
  
  
  CONCLUSIONS
  The different GSH and GST levels in patients with colorectal cancer liver metastases, liver steatosis and alcoholic cirrhosis indicate the differences in antioxidative system damage and its compensatory possibilities and could serve as potential biomarkers for its correction.
authors.affiliation: Center of Radiation and Medical Oncology, National Cancer Institute, Vilnius, Lithuania. edita.baltruskeviciene@nvi.lt.
authors.forename: Edita
authors.initials: E
authors.lastname: Baltruskeviciene
authors.forename: Birutė
authors.initials: B
authors.lastname: Kazbariene
authors.forename: Robertas
authors.initials: R
authors.lastname: Badaras
authors.forename: Loreta
authors.initials: L
authors.lastname: Bagdonaitė
authors.forename: Aurelija
authors.initials: A
authors.lastname: Krikštaponienė
authors.forename: Linas
authors.initials: L
authors.lastname: Zdanavičius
authors.forename: Eduardas
authors.initials: E
authors.lastname: Aleknavicius
authors.forename: Janina
authors.initials: J
authors.lastname: Didziapetrienė
mesh_terms: D000328:Adult; D015179:Colorectal Neoplasms / Q000097:blood* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005234:Fatty Liver / Q000097:blood* / Q000201:enzymology; D005260:Female; D005978:Glutathione / Q000097:blood*; D005982:Glutathione Transferase / Q000097:blood*; D006801:Humans; D008099:Liver / Q000473:pathology; D008104:Liver Cirrhosis, Alcoholic / Q000097:blood* / Q000201:enzymology; D008113:Liver Neoplasms / Q000097:blood* / Q000201:enzymology / Q000556:secondary; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress
chemical_list: D005982:Glutathione Transferase; D005978:Glutathione