title: Seed priming with polyethylene glycol induces antioxidative defense and metabolic regulation of rice under nano-ZnO stress.
abstract: The present study was carried out to investigate the beneficial impact of seed priming with polyethylene glycol (PEG) under different concentrations of zinc oxide nanoparticles (nano-ZnO), i.e., 0, 250, 500, and 750 mg L(-1) in two cultivars of Oryza sativa (Zhu Liang You 06 and Qian You No. 1). Physiological parameters were improved by priming with 30 % PEG in both cultivars under stress treatments. Seed priming with 30 % PEG improved α-amylase activities and total soluble sugar contents of both cultivars under nano-ZnO stress. In addition, glutathione reductase (GR) activity, reactive oxygen species (ROS) accumulation, and proline contents decreased after the priming treatment in both cultivars under different nano-ZnO concentrations. Expression of GR1, GR2, Amy2A, and Amy3A genes in shoots and roots of both cultivars increased and had higher transcription levels under the nano-ZnO stress condition. Fourier transform infrared spectroscopy (FTIR) analysis did not show any significant effects of the priming treatment on the band observed at 3400, 900, 1600, and 1000 cm(-1) corresponding to alkenyl stretch (C = C), carboxyl acid (O-H), nitrile (C = N), and aromatic (C-H), respectively, in both cultivars under nano-ZnO stress.
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.|Department of Agronomy, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt.
authors.forename: Mohamed Salah
authors.initials: MS
authors.lastname: Sheteiwy
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Qijuan
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.|Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, 60000, Pakistan.
authors.forename: Aamir
authors.initials: A
authors.lastname: Nawaz
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China. vcguan@zju.edu.cn.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Li
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China. jhu@zju.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Hu
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D053758:Nanoparticles / Q000633:toxicity*; D012275:Oryza / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D015034:Zinc Oxide / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D011092:Polyethylene Glycols; D015034:Zinc Oxide
keywords: Gene expression; Metabolic; Nano-ZnO; Oxidative stress; Seed priming