title: Fluorescent Measurement of Synaptic Activity Using SynaptopHluorin in Isolated Hippocampal Neurons.
abstract: This protocol comprises the entire process of fluorescent measurement of vesicle recycling using the probe SynaptopHluorin, a pH-dependent GFP variant whose fluorescence increases at the synapse upon vesicle release due to fluorescence quenching in acidic vesicles. This technique provides a genetic tool to monitor synaptic vesicle recycling in real time in cultured hippocampal neurons.
authors.affiliation: Department of Internal Medicine (Endocrinology), School of Medicine, Yale University, New Haven, USA.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Internal Medicine (Endocrinology), School of Medicine, Yale University, New Haven, USA.
authors.forename: Han-A
authors.initials: HA
authors.lastname: Park
authors.affiliation: Department of Internal Medicine (Endocrinology), School of Medicine, Yale University, New Haven, USA; Department of Neurobiology, School of Medicine, Yale University, New Haven, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Jonas