title: Exploration of steroidogenesis-related genes in testes, ovaries, adrenals, liver and adipose tissue in pigs.
abstract: To explore the metabolism of steroids in the pig species, a qualitative PCR analysis was performed for the main transcript of 27 genes involved in steroid metabolism. We compared samples of testes, adipose tissue and liver from immature and peripubertal males, adrenal cortex from peripubertal males, ovaries from cyclic females and adipose tissue from peripubertal females. Some genes were shown to have a tissue-specific expression. Two of them were expressed only in testes, ovaries and adrenals: CYP11A1 and CYP11B. The CYP21 and HSD17B3 genes, were expressed respectively only in adrenals and only in testes. Very few differences were observed between transcriptional patterns of peripubertal testes and adrenal glands as well as between male and female fat tissues. However, the expression of genes involved in the sulfonation of steroids was higher in testes than in adrenals from males. Main differences between ovaries and testes were observed for HSD17B1/2/3, AKR1C-pig6 and sulfotransferase genes (SULT2A1/SULT2B1). The present study shows that the SRD5A2 and CYP21 genes were not involved in the testicular biosynthesis of androstenone. It also shows that porcine adrenal glands produce essentially corticosteroids and that fat tissue is unable to produce de novo steroids.
authors.affiliation: INRA, UMR1388-GenPhySE, Castanet Tolosan, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Robic
authors.affiliation: INRA, UMR1388-GenPhySE, Castanet Tolosan, France.
authors.forename: Katia
authors.initials: K
authors.lastname: Feve
authors.affiliation: INRA, UMR1348-PEGASE, Saint-Gilles, France.|Agrocampus Ouest, Rennes, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Louveau
authors.affiliation: INRA, UMR1388-GenPhySE, Castanet Tolosan, France.
authors.forename: Juliette
authors.initials: J
authors.lastname: Riquet
authors.affiliation: INRA, UMR1348-PEGASE, Saint-Gilles, France.|Agrocampus Ouest, Rennes, France.
authors.forename: Armelle
authors.initials: A
authors.lastname: Prunier
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D015089:20-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism; D000072497:Cytochrome P450 Family 21 / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D008099:Liver / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity / Q000235:genetics; D010053:Ovary / Q000378:metabolism*; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics / Q000378:metabolism; D013256:Steroids / Q000096:biosynthesis*; D015238:Sulfotransferases / Q000235:genetics / Q000378:metabolism; D013552:Swine / Q000235:genetics* / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
chemical_list: D013256:Steroids; D015067:17-Hydroxysteroid Dehydrogenases; D015089:20-Hydroxysteroid Dehydrogenases; C115140:3 alpha-beta, 20 beta-hydroxysteroid dehydrogenase; D000072497:Cytochrome P450 Family 21; D013252:Steroid 11-beta-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme; D015238:Sulfotransferases; C028152:alcohol sulfotransferase
keywords: androgens; corticoids; estrogens; gene expression; pig