title: A robust method to screen detergents for membrane protein stabilization, revisited.
abstract: This report is a follow up of our previous paper (Lund, Orlowski, de Foresta, Champeil, le Maire and Møller (1989), J Biol Chem 264:4907-4915) showing that solubilization in detergent of a membrane protein may interfere with its long-term stability, and proposing a protocol to reveal the kinetics of such irreversible inactivation. We here clarify the fact that when various detergents are tested for their effects, special attention has of course to be paid to their critical micelle concentration. We also investigate the effects of a few more detergents, some of which have been recently advertised in the literature, and emphasize the role of lipids together with detergents. Among these detergents, lauryl maltose neopentyl glycol (LMNG) exerts a remarkable ability, even higher than that of β-dodecylmaltoside (DDM), to protect our test enzyme, the paradigmatic P-type ATPase SERCA1a from sarcoplasmic reticulum. Performing such experiments for one's favourite protein probably remains useful in pre-screening assays testing various detergents.
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Champeil
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Orlowski
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette, France.
authors.forename: Simon
authors.initials: S
authors.lastname: Babin
authors.affiliation: Medical Research Laboratory, Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark.
authors.forename: Sten
authors.initials: S
authors.lastname: Lund
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette, France.
authors.forename: Marc
authors.initials: M
authors.lastname: le Maire
authors.affiliation: Centre for Membrane Pumps in Cells and Disease - PUMPKIN, Danish National Research Foundation, Aarhus University, 8000 Aarhus, Denmark; Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark.
authors.forename: Jesper
authors.initials: J
authors.lastname: Møller
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Lenoir
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette, France. Electronic address: cedric.montigny@i2bc.paris-saclay.fr.
authors.forename: Cédric
authors.initials: C
authors.lastname: Montigny
mesh_terms: D000818:Animals; D003902:Detergents / Q000737:chemistry*; D004795:Enzyme Stability; D011817:Rabbits; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000737:chemistry*
chemical_list: D003902:Detergents; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
keywords: Detergent; LMNG; Lipid; Membrane protein; SERCA1a; Stability