title: Alterations in plasma biochemical composition in NO deficiency induced by L-NAME in mice analysed by Fourier Transform Infrared Spectroscopy.
abstract: Mouse model of nitric oxide deficiency, induced by prolonged treatment with NG-nitro-L-arginine methyl ester (L-NAME) was used for infrared spectroscopy (FTIR) analysis of plasma. L-NAME leads to increased peripheral resistance and systemic hypertension. Classification of spectral response was by principal component analysis (PCA) and linear discriminant analysis (LDA). PCA allowed to separate each animal group showing that FTIR spectra are sensitive to development of NO-deficiency on contrary to blood pressure values indicating hypertension. Globally, the most pronounced spectral alternations were observed in the second and third week of L-NAME treatment indicating that infrared signature of blood plasma can serve as indicator of early and late stages of the disease. The PLS-DA method provided >95% classification accuracy. Spectral features characteristic for L-NAME treatment were mainly associated with an elevated level of proteins accompanied by a decrease of a tyrosine content and changes in lipids/phospholipid concentration. In our work we discuss these changes for which statistically significant differences (p < 0.05 - 0.005) were observed between spectra collected for each time-point of the L-NAME treatment versus control subjects. We demonstrated for the first time that NO-deficiency and hypertension resulted in changes in biochemical profile of plasma that was detected by FTIR spectroscopy.
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland.|Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland.
authors.forename: Emilia
authors.initials: E
authors.lastname: Staniszewska-Slezak
authors.affiliation: Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Mateuszuk
authors.affiliation: Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland.|Department of Experimental Pharmacology (Chair of Pharmacology), Jagiellonian University, Grzegorzecka 16, Krakow, 31-531, Poland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Chlopicki
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland.|Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Baranska
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland. malek@chemia.uj.edu.pl.|Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland. malek@chemia.uj.edu.pl.
authors.forename: Kamilla
authors.initials: K
authors.lastname: Malek
mesh_terms: D000818:Animals; D001794:Blood Pressure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019331:NG-Nitroarginine Methyl Ester / Q000009:adverse effects*; D009569:Nitric Oxide / Q000097:blood* / Q000172:deficiency; D010949:Plasma / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D009569:Nitric Oxide; D019331:NG-Nitroarginine Methyl Ester
keywords: FTIR microspectroscopy; L-NAME mice model; NO-deficient hypertension; blood plasma