title: [Effects of BDE-209 exposure on differentiation capacity of human embryonic stem cells].
abstract: OBJECTIVE
  To study the effects of decabrominated biphenyl ether (BDE-209) exposure on differentiation capacity of human embryonic stem cells.
  
  
  METHODS
  Human embryonic stem cell line FY-hES-10 cells were exposed to the concentrations of 0 (solvent control), 1, 10 to 100 nmol/L BDE-209 for 96 h and then were differentiated into embryoid bodies (EBs) in vitro. The RNA of EBs derived from FY-hES-10 cells at Day 10 was collected. OCT4,three-layer differentiation-related genes (AFP,SMA and PAX6) and oxidative stress-related genes (HIF1a, HIF2a and SOD1) expression was detected by Q-PCR. The contents of SOD was measured by using kit.
  
  
  RESULTS
  Compared with the solvent control group, the expression levels of OCT4, SMA, HIF1a and HIF2a in BDE-209 exposure groups were increased, the expression levels of AFP, PAX6 and SOD1 were decreased, with statistically significant differences (P < 0.05). With the increase of the BDE-209 dose, the expression levels of OCT4 showed an increasing trend while the expression levels of SOD1 and the activity of the SOD showed a decreasing trend.
  
  
  CONCLUSIONS
  BDE-209 may interfere with differentiation capacity of human embryonic stem cells resulting in developmental toxicity.
authors.forename: Lili
authors.initials: L
authors.lastname: Du
authors.forename: Liyun
authors.initials: L
authors.lastname: Kuang
authors.forename: Wenting
authors.initials: W
authors.lastname: Tang
authors.forename: Dunjin
authors.initials: D
authors.lastname: Chen
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D005136:Eye Proteins / Q000378:metabolism; D055768:Halogenated Diphenyl Ethers / Q000494:pharmacology*; D018398:Homeodomain Proteins / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000187:drug effects*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000378:metabolism; D012333:RNA, Messenger; D012097:Repressor Proteins / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D005136:Eye Proteins; D055768:Halogenated Diphenyl Ethers; D018398:Homeodomain Proteins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D050814:Octamer Transcription Factor-3; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012333:RNA, Messenger; D012097:Repressor Proteins; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000099162:Endothelial PAS Domain-Containing Protein 1; C497442:HIF1A protein, human; C000605730:PAX6 protein, human; C494173:POU5F1 protein, human; C000606290:SOD1 protein, human; C010902:decabromobiphenyl ether