title: A consolidated method for screening the endocrine activity of drinking water.
abstract: Endocrine activity of drinking water is a matter of growing interest for scientists as well as health authorities. A concentration technique for endocrine activity screening was developed, optimized, and transposed from 200mL to 10L water samples. To avoid any contamination during concentration, the method was developed using exclusively glass, Teflon and stainless steel materials. Any potential losses were tracked using three model radiolabeled molecules, namely BPA, DEHP and 4n-NP. The final method allowed 10L water samples to be concentrated 5000-fold, with good recovery and repeatability. After validation, by concentrating spiked and non-spiked 10L samples of EVIAN natural mineral water, 14 different drinking water samples were concentrated and screened for endocrine disrupting activity using bioluminescent assays. Samples consisting of bottled water, conditioned in various materials (glass, PET) and subjected to different storage conditions, had no hormone-like activities whereas estrogenic activity was found in the filtered tap water.
authors.affiliation: TOXALIM, Université de Toulouse, INRA, INP-ENVT, INP-EI-Purpan, Univ. Toulouse 3 Paul Sabatier, F-31027 Toulouse, France; MetaToul-AXIOM Platform, MetaToul-MetaboHUB, National Infrastructure for Metabolomics and Fluxomics, F-31027 Toulouse, France. Electronic address: sylvie.chevolleau@toulouse.inra.fr.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Chevolleau
authors.affiliation: TOXALIM, Université de Toulouse, INRA, INP-ENVT, INP-EI-Purpan, Univ. Toulouse 3 Paul Sabatier, F-31027 Toulouse, France; MetaToul-AXIOM Platform, MetaToul-MetaboHUB, National Infrastructure for Metabolomics and Fluxomics, F-31027 Toulouse, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Debrauwer
authors.affiliation: Danone Nutricia Research, RD128, 91767 Palaiseau, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Stroheker
authors.affiliation: Danone Waters Analytical Center, Rue des Vignes Rouge, 74506 Amphion, France.
authors.forename: Liza
authors.initials: L
authors.lastname: Viglino
authors.affiliation: TOXALIM, Université de Toulouse, INRA, INP-ENVT, INP-EI-Purpan, Univ. Toulouse 3 Paul Sabatier, F-31027 Toulouse, France; MetaToul-AXIOM Platform, MetaToul-MetaboHUB, National Infrastructure for Metabolomics and Fluxomics, F-31027 Toulouse, France.
authors.forename: Issam
authors.initials: I
authors.lastname: Mourahib
authors.affiliation: TOXALIM, Université de Toulouse, INRA, INP-ENVT, INP-EI-Purpan, Univ. Toulouse 3 Paul Sabatier, F-31027 Toulouse, France; MetaToul-AXIOM Platform, MetaToul-MetaboHUB, National Infrastructure for Metabolomics and Fluxomics, F-31027 Toulouse, France.
authors.forename: Maria-Helena
authors.initials: MH
authors.lastname: Meireles
authors.affiliation: IRCM, INSERM U1194, Université de Montpellier, ICM, Montpellier F-34298, France.
authors.forename: Marina
authors.initials: M
authors.lastname: Grimaldi
authors.affiliation: IRCM, INSERM U1194, Université de Montpellier, ICM, Montpellier F-34298, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Balaguer
authors.affiliation: Danone Nutricia Research, RD128, 91767 Palaiseau, France.
authors.forename: Lodovico
authors.initials: L
authors.lastname: di Gioia
mesh_terms: D060766:Drinking Water / Q000737:chemistry*; D052244:Endocrine Disruptors / Q000032:analysis*; D004970:Estrone; D008163:Luminescent Measurements; D008900:Mineral Waters
chemical_list: D060766:Drinking Water; D052244:Endocrine Disruptors; D008900:Mineral Waters; D004970:Estrone
keywords: Drinking water; Endocrine disrupting activity; Radiolabeled compounds; Sample concentration