title: Simultaneous Phenotypic and Genotypic Studies of Individual Mitotic Cells-Methods and Practical Applications.
abstract: Recently established techniques for simultaneous immunological and cytogenetic characterization of mitotic cell populations have opened further possibilities for the classification of certain cells in haematological neoplasias. These techniques have been termed MAC (morphology-antibody- chromosomes). Several staining procedures e.g. immunogold, immunofluorescence or immunoenzymatic techniques for simultaneous and/or consecutive cytogenetic analyses have been established and were applied for certain investigations. The advantages and disadvantages of these methods are discussed, as well as th: results which have been published until now, and future aspects of these techniques.
authors.affiliation: a Institute of Immunology, University of Vienna, Austria.
authors.forename: U
authors.initials: U
authors.lastname: Köller
authors.affiliation: b Children's Cancer Research Institute, St. Anna Children's Hospital, Vienna, Austria.
authors.forename: O A
authors.initials: OA
authors.lastname: Haas
authors.affiliation: a Institute of Immunology, University of Vienna, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Gaidnerp
authors.affiliation: a Institute of Immunology, University of Vienna, Austria.
authors.forename: W
authors.initials: W
authors.lastname: Knapp
keywords: Simultaneous cell characterisation; immunophenotyping chromosome banding