title: Complete genotyping of unusual species A rotavirus G12P[11] and G10P[14] isolates and evidence of frequent in vivo reassortment among the rotaviruses detected in children with diarrhea in Kolkata, India, during 2014.
abstract: Species A rotaviruses (RVA) are the most important cause of acute gastroenteritis in the young of humans and many animal species globally. G1P[8], G2P[4], G3P[8], G4P[8], G9P[6/8] and G12P[6/8] are the predominantly isolated genotypes throughout the world including India. Unusual genotypes from different host species such as G5, G6, G8, G10 and G11 have also been reported in humans with low frequency. In the present study, among >650 RVA positive stool samples collected from children with diarrhea in Kolkata, India, during 2014, two isolates each of the genotype G12P[11] and G10P[14] were obtained and their genomes completely sequenced. The full genotype constellations were G12-P[11]-I1-R1-C1-M2-A1-N1-T2-E1-H1 and G12-P[11]-I1-R1-C1-M1-A5-N1-T1-E1-H1 for G12P[11] viruses, suggesting several reassortments between Wa- and DS-1-like human RVA strains, including possible reassortment of a simian NSP1 gene. The G10P[14] viruses (G10-P[14]-I2-R2-C2-M2-A11-N2-T6-E2-H3) were found to contain multiple genes closely related to RVAs of artiodactyl origin, highlighting the role of inter-host species transmissions of RVAs. From the G/P constellation of all RVA isolates, it could be concluded that approximately one quarter had likely arisen from reassortment events in vivo among RVAs of 'usual' genotypes.
authors.affiliation: National Institute of Cholera and Enteric Diseases, Kolkata, 700010, India.
authors.forename: Paulami
authors.initials: P
authors.lastname: Mandal
authors.affiliation: National Institute of Cholera and Enteric Diseases, Kolkata, 700010, India.
authors.forename: Satarupa
authors.initials: S
authors.lastname: Mullick
authors.affiliation: National Institute of Cholera and Enteric Diseases, Kolkata, 700010, India.
authors.forename: Mukti Kant
authors.initials: MK
authors.lastname: Nayak
authors.affiliation: National Institute of Cholera and Enteric Diseases, Kolkata, 700010, India.
authors.forename: Anupam
authors.initials: A
authors.lastname: Mukherjee
authors.affiliation: Institute of Child Health, Kolkata, India.
authors.forename: Nupur
authors.initials: N
authors.lastname: Ganguly
authors.affiliation: Institute of Child Health, Kolkata, India.
authors.forename: Prabal
authors.initials: P
authors.lastname: Niyogi
authors.affiliation: National Institute of Cholera and Enteric Diseases, Kolkata, 700010, India.
authors.forename: Samiran
authors.initials: S
authors.lastname: Panda
authors.affiliation: Division of Virology, National Institute of Cholera and Enteric Diseases, P-33 C.I.T. Road, Scheme XM, Beliaghata, Kolkata, 700010, West Bengal, India. sarkarmc.niced@gov.in.
authors.forename: Mamta
authors.initials: M
authors.lastname: Chawla-Sarkar
mesh_terms: D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D019143:Evolution, Molecular; D005243:Feces / Q000821:virology; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral