title: Molecular identification and phylogenetical analysis of dermatophyte fungi from Latin America.
abstract: Dermatophytes constitute a complex group of fungi, comprised of by the genera Trichophyton, Epidermophyton and Microsporum. They have the ability to degrade keratin and cause human and animal infections. Molecular techniques have made their identification faster and more accurate, and allowed important advances in phylogenetic studies. We aim to identify molecularly and to determine the phylogenetic relationships in dermatophyte fungi from Brazil and other Latin American countries, using DNA sequencing of the nuclear ribosome regions ITS1-5.8S-ITS2 and D1/D2. DNA of 45 dermatophytes was extracted and amplified by PCR for identification at the species level by sequencing of those ribosomal regions. The software mega 6.0 was used to establish the phylogenetic relationships via the Maximum Likelihood method. Out of 45 strains, 43 were identified by ITS (95.5%) and 100% by D1/D2 sequencing. Two strains could not be identified by ITS. Phylogenetic analyses separated the genera Trichophyton and Microsporum, which presented an uncertain relationship with Epidermophyton floccosum, depending on the ribosomal marker. Both regions can provide efficient identification of dermatophytes, whereas phylogenetic analysis revealed complex relations among dermatophyte fungi.
authors.affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, São Paulo, Brazil.
authors.forename: Hans
authors.initials: H
authors.lastname: Garcia Garces
authors.affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, São Paulo, Brazil.
authors.forename: Marluce F
authors.initials: MF
authors.lastname: Hrycyk
authors.affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, São Paulo, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Giacobino
authors.affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, São Paulo, Brazil.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Capela Machado
authors.affiliation: Centro de Biociências, Universidade Federal de Rio Grande do Norte, Natal, Brazil.
authors.forename: Thales
authors.initials: T
authors.lastname: Domingos Arantes
authors.affiliation: Centro de Biociências, Universidade Federal de Rio Grande do Norte, Natal, Brazil.
authors.forename: Raquel C
authors.initials: RC
authors.lastname: Theodoro
authors.affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, São Paulo, Brazil.
authors.forename: Sandra de M G
authors.initials: SMG
authors.lastname: Bosco
authors.affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, São Paulo, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Bagagli
mesh_terms: D003883:Arthrodermataceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003881:Dermatomycoses / Q000382:microbiology*; D006801:Humans; D007843:Latin America; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
keywords: Arthrodermataceae; Dermatophytes; molecular identification; phylogeny