title: A Novel Peptide from Soybean Protein Isolate Significantly Enhances Resistance of the Organism under Oxidative Stress.
abstract: Recent studies have indicated that protein hydrolysates have broad biological effects. In the current study we describe a novel antioxidative peptide, FDPAL, from soybean protein isolate (SPI). The aim of this study was to purify and characterize an antioxidative peptide from SPI and determine its antioxidative mechanism. LC-MS/MS was used to isolate and identify the peptide from SPI. The sequence of the peptide was determined to be Phe-Asp-Pro-Ala-Leu (FDPAL, 561 Da). FDPAL can cause significant enhancement of resistance to oxidative stress both in cells as well as simple organisms. In Caenorhabditis elegans (C. elegans), FDPAL can up-regulate the expression of certain genes associated with resistance. The antioxidant activity of this peptide can be attributed to the presence of a specific amino acid sequence. Results from our work suggest that FDPAL can facilitate potential applications of proteins carrying this sequence in the nutraceutical, bioactive material and clinical medicine areas, as well as in cosmetics and health care products.
authors.affiliation: Key laboratory for Molecular Enzymology and Engineering, the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, 130012, P.R. China.
authors.forename: Heran
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of endocrinology, China-Japan Union Hospital, Jilin University, Changchun, Jilin, 130033, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Key laboratory for Molecular Enzymology and Engineering, the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, 130012, P.R. China.
authors.forename: Ziyuan
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Key laboratory for Molecular Enzymology and Engineering, the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, 130012, P.R. China.
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key laboratory for Molecular Enzymology and Engineering, the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, 130012, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Sports Science Research Institute of Jilin Province, Changchun, Jilin, 130022, P. R. China.
authors.forename: Yudan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Key laboratory for Molecular Enzymology and Engineering, the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, 130012, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Key laboratory for Molecular Enzymology and Engineering, the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, 130012, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D017173:Caenorhabditis elegans; D002845:Chromatography; D005786:Gene Expression Regulation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017382:Reactive Oxygen Species; D030262:Soybean Proteins / Q000737:chemistry / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D001688:Biological Products; D010455:Peptides; D017382:Reactive Oxygen Species; D030262:Soybean Proteins; D013482:Superoxide Dismutase