title: Plasma long non-coding RNA MALAT1 is associated with distant metastasis in patients with epithelial ovarian cancer.
abstract: Human metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a newly identified metastasis-associated long non-coding RNA. In a previous study, it was identified that plasma levels of MALAT1 were significantly increased in gastric cancer patients with metastasis compared with gastric cancer patients without metastasis and healthy control individuals. However, it is unclear whether plasma levels of MALAT1 may act as a biomarker for evaluating the development of metastasis in epithelial ovarian cancer (EOC). In the present study, groups that consisted of 47 patients with EOC and metastasis (EOC/DM), 47 patients with EOC without metastasis (EOC/NDM), and 47 healthy control (HC) individuals were established. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the level of plasma MALAT1 in these groups. The results showed that levels of plasma MALAT1 were significantly increased in the EOC/DM group compared with the EOC/NDM and HC groups (P<0.001). Receiver operating characteristic (ROC) analysis indicated that plasma MALAT1 yielded an area under the curve (AUC) of 0.820 [95% confidence interval (CI), 0.734-0.905; P<0.001], distinguishing between EOC/DM and EOC/NDM. ROC analysis also yielded an AUC of 0.884 (95% CI, 0.820-0.949; P<0.001), with 89.4% sensitivity and 72.3% specificity for distinguishing between EOC/DM and HC. Furthermore, multivariate analysis indicated that overexpression of MALAT1, differentiation (poor), tumor-node-metastasis stage (IV), lymph node metastasis (N3), peritoneal invasion (present) and higher serum carbohydrate antigen 125 levels were independent predictors of survival (hazard ratio, 3.322; P=0.028) in patients with EOC. Kaplan-Meier analysis revealed that patients with increased MALAT1 expression had a poorer disease-free survival time. In conclusion, the levels of plasma MALAT1 may act as a valuable biomarker for the diagnosis of metastasis.
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Qingjuan
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Yongyong
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Weian
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Weibo
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Si
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Bingwei
authors.initials: B
authors.lastname: Dong
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Lianying
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Ximin
authors.initials: X
authors.lastname: Qiao
authors.affiliation: Department of Medical Oncology, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Zhang
keywords: MALAT1; distant metastasis; epithelial ovarian cancer; long non-coding RNA