title: Isolation of Mouse Hair Follicle Bulge Stem Cells and Their Functional Analysis in a Reconstitution Assay.
abstract: The hair follicle (HF) is a dynamic structure readily accessible within the skin, and contains various pools of stem cells that have a broad regenerative potential during normal homeostasis and in response to injury. Recent discoveries demonstrating the multipotent capabilities of hair follicle stem cells and the easy access to skin tissue make the HF an attractive source for isolating stem cells and their subsequent application in tissue engineering and regenerative medicine. Here, we describe the isolation and purification of hair follicle bulge stem cells from mouse skin, and hair reconstitution assays that allows the functional analysis of multipotent stem cells.
authors.affiliation: Department of Dermatology, University of Pennsylvania Medical Center, Philadelphia, PA, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Dermatology, University of Pennsylvania Medical Center, Philadelphia, PA, USA.
authors.forename: Jen-Chih
authors.initials: JC
authors.lastname: Hsieh
authors.affiliation: Department of Dermatology and Cutaneous Surgery, University of Miami, Miller School of Medicine, Miami, FL, USA.
authors.forename: Julia
authors.initials: J
authors.lastname: Escandon
authors.affiliation: Department of Dermatology, University of Pennsylvania Medical Center, Philadelphia, PA, USA. cotsarel@mail.med.upenn.edu.
authors.forename: George
authors.initials: G
authors.lastname: Cotsarelis
mesh_terms: D000818:Animals; D015415:Biomarkers; D002469:Cell Separation; D020405:Dermis / Q000166:cytology; D006197:Hair; D018859:Hair Follicle / Q000166:cytology*; D016130:Immunophenotyping; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D012038:Regeneration
chemical_list: D015415:Biomarkers
keywords: Bulge; Hair follicle stem cell; Hair reconstitution assay; Regeneration