title: Metabotyping human endometrioid endometrial adenocarcinoma reveals an implication of endocannabinoid metabolism.
abstract: Metabolomics, an essential technique in precision medicine, contributes to the molecular fingerprinting of tumours, further helping to understand their pathogenesis. In this work, using a LC-ESI-QTOF-MS/MS platform, we demonstrated the existence of a specific metabolomic signature which could define endometrioid endometrial carcinoma (EEC), arising the endocannabinoid system as a potential pathway involved in EC pathogenesis. Metabolomics could also shed light in the processes involved in myometrial invasion, proposing new targets for possible therapeutic intervention. Consequently, we also described a different metabolomic profile in surface endometrioid carcinoma and myometrial invasive front. We validated pathways disclosed by metabolomics by immunohistochemistry. Specifically, endocannabinoid and purine metabolism could be involved in tumor myometrial invasion.
authors.affiliation: Department of Experimental Medicine, Universitat de Lleida, IRBLleida, E-25198, Lleida, Spain.
authors.forename: Mariona
authors.initials: M
authors.lastname: Jové
authors.affiliation: Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Hospital Universitari Arnau de Vilanova, Universitat de Lleida, IRBLleida, E-25198, Lleida, Spain.
authors.forename: Sònia
authors.initials: S
authors.lastname: Gatius
authors.affiliation: Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Hospital Universitari Arnau de Vilanova, Universitat de Lleida, IRBLleida, E-25198, Lleida, Spain.
authors.forename: Andree
authors.initials: A
authors.lastname: Yeramian
authors.affiliation: Department of Experimental Medicine, Universitat de Lleida, IRBLleida, E-25198, Lleida, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Portero-Otin
authors.affiliation: Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Hospital Universitari Arnau de Vilanova, Universitat de Lleida, IRBLleida, E-25198, Lleida, Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Eritja
authors.affiliation: Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Hospital Universitari Arnau de Vilanova, Universitat de Lleida, IRBLleida, E-25198, Lleida, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Santacana
authors.affiliation: Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Hospital Universitari Arnau de Vilanova, Universitat de Lleida, IRBLleida, E-25198, Lleida, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Colas
authors.affiliation: Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Hospital Universitari Arnau de Vilanova, Universitat de Lleida, IRBLleida, E-25198, Lleida, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Ruiz
authors.affiliation: Department of Experimental Medicine, Universitat de Lleida, IRBLleida, E-25198, Lleida, Spain.
authors.forename: Reinald
authors.initials: R
authors.lastname: Pamplona
authors.affiliation: Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Hospital Universitari Arnau de Vilanova, Universitat de Lleida, IRBLleida, E-25198, Lleida, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Matias-Guiu
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D018269:Carcinoma, Endometrioid / Q000378:metabolism*; D063388:Endocannabinoids / Q000378:metabolism*; D016889:Endometrial Neoplasms / Q000378:metabolism*; D005260:Female; D006801:Humans; D055432:Metabolomics / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D063388:Endocannabinoids
keywords: endocannabinoid system; endometrioid endometrial carcinoma; mass spectrometry; metabolomic profile; personalized medicine