title: Effects of resveratrol on carbon monoxide-induced cardiotoxicity in rats.
abstract: Carbon monoxide (CO) poisoning leads to tissue hypoxia resulting in cardiovascular disturbances. Resveratrol (RES) is considered a natural cardioprotective agent especially in the setting of ischemia/reperfusion injury. In the present study, the cardioprotective potential of RES against CO-induced cardiotoxicity was evaluated. 45 male Wistar rats, animals were randomly assigned to 5 experimental groups. The first group served as negative control and was not exposed to CO. All remaining rats were exposed to CO 3000ppm for 60min. The second group received normal saline following CO exposure, while groups 3, 4 and 5 were injected intraperitoneally with different doses of RES (1, 5 and 10mg/kg, respectively). Histopathological examination showed that RES administration reduced myocardial lesions compared to control groups. Myocardial Akt expression was significantly increased in rats treated with the highest dose of RES (p<0.05) compared to CO-exposed non-treated animals. Caspase-3 activity in rat cardiomyocytes of RES-treated animals was significantly decreased in a dose-dependent manner. ECG findings did not differ significantly among CO-exposed groups. In conclusion, the present study offers evidence of a protective effect of RES administration on CO-induced cardiotoxicity via Akt up-regulation and attenuation of caspase-3 activity in rat hearts.
authors.affiliation: Department of Pharmacodynamics and Toxicology, School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran; Students Research Committee, School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Hashemzaei
authors.affiliation: Department of Pharmacodynamics and Toxicology, School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.
authors.forename: Arian Khandan
authors.initials: AK
authors.lastname: Barani
authors.affiliation: Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Iranshahi
authors.affiliation: Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran; Department of Forensic Sciences and Toxicology, Faculty of Medicine, University of Crete, Heraklion 71003, Greece.
authors.forename: Ramin
authors.initials: R
authors.lastname: Rezaee
authors.affiliation: Cardiology Clinic, University General Hospital of Larisa, Thessaly, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Tsarouhas
authors.affiliation: Department of Forensic Sciences and Toxicology, Faculty of Medicine, University of Crete, Heraklion 71003, Greece.
authors.forename: Aristidis Michael
authors.initials: AM
authors.lastname: Tsatsakis
authors.affiliation: Swiss Centre for Applied Human Toxicology, University of Basel, Switzerland.
authors.forename: Martin F
authors.initials: MF
authors.lastname: Wilks
authors.affiliation: Department of Pharmacodynamics and Toxicology, School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran; Students Research Committee, School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran. Electronic address: ttoxicologist@gmail.com.
authors.forename: Kaveh
authors.initials: K
authors.lastname: Tabrizian
mesh_terms: D000818:Animals; D002248:Carbon Monoxide / Q000008:administration & dosage / Q000633:toxicity*; D002249:Carbon Monoxide Poisoning / Q000517:prevention & control; D002263:Carboxyhemoglobin / Q000378:metabolism; D002316:Cardiotonic Agents / Q000494:pharmacology*; D066126:Cardiotoxicity / Q000209:etiology; D053148:Caspase 3 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004562:Electrocardiography; D006321:Heart / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D002316:Cardiotonic Agents; D013267:Stilbenes; D002248:Carbon Monoxide; D002263:Carboxyhemoglobin; D051057:Proto-Oncogene Proteins c-akt; D053148:Caspase 3; D000077185:Resveratrol
keywords: Akt; Apoptosis; Carbon monoxide poisoning; Cardiotoxicity; Electrocardiogram; Rat; Resveratrol