title: Identification of miRNAs Involved in Stolon Formation in Tulipa edulis by High-Throughput Sequencing.
abstract: MicroRNAs (miRNAs) are a class of endogenous, non-coding small RNAs that play an important role in transcriptional and post-transcriptional gene regulation. However, the sequence information and functions of miRNAs are still unexplored in Tulipa edulis. In this study, high-throughput sequencing was used to identify small RNAs in stolon formation stages (stage 1, 2, and 3) in T. edulis. A total of 12,890,912, 12,182,122, and 12,061,434 clean reads were obtained from stage 1, 2, and 3, respectively. Among the reads, 88 conserved miRNAs and 70 novel miRNAs were identified. Target prediction of 122 miRNAs resulted in 531 potential target genes. Nr, Swiss-Prot, GO, COG, and KEGG annotations revealed that these target genes participate in many biologic and metabolic processes. Moreover, qRT-PCR was performed to analyze the expression levels of the miRNAs and target genes in stolon formation. The results revealed that miRNAs play a key role in T. edulis stolon formation.
authors.affiliation: Institute of Chinese Medicinal Materials, Nanjing Agricultural University Nanjing, China.
authors.forename: Zaibiao
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Institute of Chinese Medicinal Materials, Nanjing Agricultural University Nanjing, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Institute of Chinese Medicinal Materials, Nanjing Agricultural University Nanjing, China.
authors.forename: Qiaosheng
authors.initials: Q
authors.lastname: Guo
authors.affiliation: College of Pharmacy, Henan University of Chinese Medicine Zhengzhou, China.
authors.forename: Yunhao
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Institute of Chinese Medicinal Materials, Nanjing Agricultural University Nanjing, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Chinese Medicinal Materials, Nanjing Agricultural University Nanjing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sun
keywords: Tulipa edulis; gene expression; high-throughput sequencing; miRNAs; stolon formation