title: [QUANTITATIVE DNA EVALUATION OF THE HIGH CARCINOGENIC RISK OF HUMAN PAPILLOMA VIRUSES AND HUMAN HERPES VIRUSES IN MALES WITH FERTILITY DISORDERS].
abstract: Infertility is an actual medical and social problem. In 50% of couples it is associated with the male factor and in more than 50% of cases the etiology of the infertility remains insufficiently understood. The goal of this work was to study the prevalence and to perform quantitative analysis of the human herpes viruses (HHV) and high carcinogenic risk papilloma viruses (HR HPV) in males with infertility, as well as to assess the impact of these infections on sperm parameters. Ejaculate samples obtained from 196 males fall into 3 groups. Group 1 included men with the infertility of unknown etiology (n = 112); group 2, patients who had female partners with the history of spontaneous abortion (n = 63); group 3 (control), healthy men (n = 21). HHV and HR HPV DNA in the ejaculates were detected in a total of 42/196 (21.4%) males: in 31 and 11 patients in groups 1 and 2, respectively (p > 0.05) and in none of healthy males. HHV were detected in 24/42; HR HPV, in 18/42 males (p > 0.05) without significant difference between the groups. Among HR HPV genotypes of the clade A9 in ejaculate were more frequent (14/18, p = 0.04). Comparative analysis of the sperm parameters showed that in the ejaculates of the infected patients sperm motility as well as the number of morphologically normal cells were significantly reduced compared with the healthy men. The quantification of the viral DNA revealed that in 31% of the male ejaculates the viral load was high: > 3 Ig10/100000 cells. Conclusion. The detection of HHV and HR HPV in the ejaculate is associated with male infertility. Quantification of the viral DNA in the ejaculate is a useful indicator for monitoring viral infections in infertility and for decision to start therapy.
authors.forename: V V
authors.initials: VV
authors.lastname: Evdokimov
authors.forename: V A
authors.initials: VA
authors.lastname: Naumenko
authors.forename: Yu A
authors.initials: YA
authors.lastname: Tulenev
authors.forename: L F
authors.initials: LF
authors.lastname: Kurilo
authors.forename: V P
authors.initials: VP
authors.lastname: Kovalyk
authors.forename: T M
authors.initials: TM
authors.lastname: Sorokina
authors.forename: A L
authors.initials: AL
authors.lastname: Lebedeva
authors.forename: M A
authors.initials: MA
authors.lastname: Gomberg
authors.forename: A A
authors.initials: AA
authors.lastname: Kushch
mesh_terms: D000022:Abortion, Spontaneous / Q000473:pathology; D000328:Adult; D016022:Case-Control Studies; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D005260:Female; D006564:Herpesviridae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D006566:Herpesviridae Infections / Q000150:complications / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D006801:Humans; D007248:Infertility, Male / Q000150:complications / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D008297:Male; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D012306:Risk; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000473:pathology / Q000821:virology*; D019562:Viral Load
chemical_list: D004279:DNA, Viral