title: Three-dimensional registration of intravascular optical coherence tomography and cryo-image volumes for microscopic-resolution validation.
abstract: Evidence suggests high-resolution, high-contrast, [Formula: see text] intravascular optical coherence tomography (IVOCT) can distinguish plaque types, but further validation is needed, especially for automated plaque characterization. We developed experimental and three-dimensional (3-D) registration methods to provide validation of IVOCT pullback volumes using microscopic, color, and fluorescent cryo-image volumes with optional registered cryo-histology. A specialized registration method matched IVOCT pullback images acquired in the catheter reference frame to a true 3-D cryo-image volume. Briefly, an 11-parameter registration model including a polynomial virtual catheter was initialized within the cryo-image volume, and perpendicular images were extracted, mimicking IVOCT image acquisition. Virtual catheter parameters were optimized to maximize cryo and IVOCT lumen overlap. Multiple assessments suggested that the registration error was better than the [Formula: see text] spacing between IVOCT image frames. Tests on a digital synthetic phantom gave a registration error of only [Formula: see text] (signed distance). Visual assessment of randomly presented nearby frames suggested registration accuracy within 1 IVOCT frame interval ([Formula: see text]). This would eliminate potential misinterpretations confronted by the typical histological approaches to validation, with estimated 1-mm errors. The method can be used to create annotated datasets and automated plaque classification methods and can be extended to other intravascular imaging modalities.
authors.affiliation: Case Western Reserve University , Department of Biomedical Engineering, Cleveland, 10900 Euclid Ave, Cleveland, Ohio 44106, United States.
authors.forename: David
authors.initials: D
authors.lastname: Prabhu
authors.affiliation: University Hospitals Case Medical Center , Harrington Heart and Vascular Institute, Cardiovascular Imaging Core Laboratory, 11100 Euclid Avenue, Cleveland, Ohio 44106, United States.
authors.forename: Emile
authors.initials: E
authors.lastname: Mehanna
authors.affiliation: Case Western Reserve University , Department of Biomedical Engineering, Cleveland, 10900 Euclid Ave, Cleveland, Ohio 44106, United States.
authors.forename: Madhusudhana
authors.initials: M
authors.lastname: Gargesha
authors.affiliation: University Hospitals Case Medical Center , Harrington Heart and Vascular Institute, Cardiovascular Imaging Core Laboratory, 11100 Euclid Avenue, Cleveland, Ohio 44106, United States.
authors.forename: Eric
authors.initials: E
authors.lastname: Brandt
authors.affiliation: University Hospitals Case Medical Center, Harrington Heart and Vascular Institute, Cardiovascular Imaging Core Laboratory, 11100 Euclid Avenue, Cleveland, Ohio 44106, United States; Case Western Reserve University, Department of Biomedical Engineering, Cleveland, 10900 Euclid Ave, Cleveland, Ohio 44106, United States.
authors.forename: Di
authors.initials: D
authors.lastname: Wen
authors.affiliation: University Hospitals Case Medical Center , Harrington Heart and Vascular Institute, Cardiovascular Imaging Core Laboratory, 11100 Euclid Avenue, Cleveland, Ohio 44106, United States.
authors.forename: Nienke S
authors.initials: NS
authors.lastname: van Ditzhuijzen
authors.affiliation: University Hospitals Case Medical Center , Harrington Heart and Vascular Institute, Cardiovascular Imaging Core Laboratory, 11100 Euclid Avenue, Cleveland, Ohio 44106, United States.
authors.forename: Daniel
authors.initials: D
authors.lastname: Chamie
authors.affiliation: University Hospitals Case Medical Center , Harrington Heart and Vascular Institute, Cardiovascular Imaging Core Laboratory, 11100 Euclid Avenue, Cleveland, Ohio 44106, United States.
authors.forename: Hirosada
authors.initials: H
authors.lastname: Yamamoto
authors.affiliation: University Hospitals Case Medical Center , Harrington Heart and Vascular Institute, Cardiovascular Imaging Core Laboratory, 11100 Euclid Avenue, Cleveland, Ohio 44106, United States.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Fujino
authors.affiliation: University Hospitals Case Medical Center , Harrington Heart and Vascular Institute, Cardiovascular Imaging Core Laboratory, 11100 Euclid Avenue, Cleveland, Ohio 44106, United States.
authors.forename: Ali
authors.initials: A
authors.lastname: Alian
authors.affiliation: Case Western Reserve University , Department of Biomedical Engineering, Cleveland, 10900 Euclid Ave, Cleveland, Ohio 44106, United States.
authors.forename: Jaymin
authors.initials: J
authors.lastname: Patel
authors.affiliation: University Hospitals Case Medical Center , Harrington Heart and Vascular Institute, Cardiovascular Imaging Core Laboratory, 11100 Euclid Avenue, Cleveland, Ohio 44106, United States.
authors.forename: Marco
authors.initials: M
authors.lastname: Costa
authors.affiliation: University Hospitals Case Medical Center , Harrington Heart and Vascular Institute, Cardiovascular Imaging Core Laboratory, 11100 Euclid Avenue, Cleveland, Ohio 44106, United States.
authors.forename: Hiram G
authors.initials: HG
authors.lastname: Bezerra
authors.affiliation: Case Western Reserve University , Department of Biomedical Engineering, Cleveland, 10900 Euclid Ave, Cleveland, Ohio 44106, United States.
authors.forename: David L
authors.initials: DL
authors.lastname: Wilson
keywords: cryo-imaging; histology; intravascular imaging; optical coherence tomography; three-dimensional image registration; validation