title: Accumulation of arachidonic acid-containing phosphatidylinositol at the outer edge of colorectal cancer.
abstract: Accumulating evidence indicates that cancer cells show specific alterations in phospholipid metabolism that contribute to tumour progression in several types of cancer, including colorectal cancer. Questions still remain as to what lipids characterize the outer edge of cancer tissues and whether those cancer outer edge-specific lipid compositions emerge autonomously in cancer cells. Cancer tissue-originated spheroids (CTOSs) that are composed of pure primary cancer cells have been developed. In this study, we aimed to seek out the cancer cell-autonomous acquisition of cancer outer edge-characterizing lipids in colorectal cancer by analysing phospholipids in CTOSs derived from colorectal cancer patients with matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS). A signal at m/z 885.5 in negative ion mode was detected specifically at the surface regions. The signal was identified as an arachidonic acid (AA)-containing phosphatidylinositol (PI), PI(18:0/20:4), by tandem mass spectrometry analysis. Quantitative analysis revealed that the amount of PI(18:0/20:4) in the surface region of CTOSs was two-fold higher than that in the medial region. Finally, PI(18:0/20:4) was enriched at the cancer cells/stromal interface in colorectal cancer patients. These data imply a possible importance of AA-containing PI for colorectal cancer progression, and suggest cells expressing AA-containing PI as potential targets for anti-cancer therapy.
authors.affiliation: Second Department of Surgery, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.|Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Hiraide
authors.affiliation: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.|International Mass Imaging Center, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Ikegami
authors.affiliation: Second Department of Surgery, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Sakaguchi
authors.affiliation: Second Department of Surgery, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Morita
authors.affiliation: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hayasaka
authors.affiliation: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.|International Mass Imaging Center, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Noritaka
authors.initials: N
authors.lastname: Masaki
authors.affiliation: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Michihiko
authors.initials: M
authors.lastname: Waki
authors.affiliation: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.|International Mass Imaging Center, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Sugiyama
authors.affiliation: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Shinriki
authors.affiliation: Second Department of Surgery, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.|Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Takeda
authors.affiliation: Second Department of Surgery, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.|Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shibasaki
authors.affiliation: Second Department of Surgery, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Miyazaki
authors.affiliation: Second Department of Surgery, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Kikuchi
authors.affiliation: Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari-ku, Osaka, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okuyama
authors.affiliation: Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari-ku, Osaka, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Inoue
authors.affiliation: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.|International Mass Imaging Center, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.|Preeminent Medical Photonics Education &Research Center, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.|Department of Anatomy, The University of Hong Kong, Pokfulam, Hong Kong SAR.|Division of Neural Systematics, National Institute for Physiological Sciences, Myodaiji, Okazaki, Japan.|Riken Center for Molecular Imaging Science, Chuo-ku, Kobe, Japan.
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
authors.affiliation: Second Department of Surgery, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Konno
mesh_terms: D000368:Aged; D016718:Arachidonic Acid / Q000378:metabolism*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D010716:Phosphatidylinositols / Q000378:metabolism*; D018874:Spheroids, Cellular / Q000378:metabolism
chemical_list: D010716:Phosphatidylinositols; D016718:Arachidonic Acid