title: Artificial plasma membrane models based on lipidomic profiling.
abstract: Phospholipid monolayers are often described as membrane models for analyzing drug-lipid interactions. In many works, a single phosphatidylcholine is chosen, sometimes with one or two additional components. Drug penetration is studied at 30mN/m, a surface pressure considered as corresponding to the pressure in bilayers, independently of the density of lipid molecular packing. In this work, we have extracted, identified, and quantified the major lipids constituting the lipidome of plasma and mitochondrial membranes of retinoblastoma (Y79) and retinal pigment epithelium cells (ARPE-19), using liquid chromatography coupled to high-resolution mass spectrometry (LC-MS/MS). The results obtained from this lipidomic analysis were used in an attempt to build an artificial lipid monolayer with a composition mimicking that of the plasma membrane of Y79 cells, better than a single phospholipid. The variety and number of lipid classes and species in cell extracts monolayers exceeding by far those of the phospholipids chosen to mimic them, the π-A isotherms of model monolayers differed from those of lipid extracts in shape and apparent packing density. We propose a model monolayer based on the most abundant species identified in the extracts, with a surface compressional modulus at 30mN/m close to the one of the lipid extracts.
authors.affiliation: Institut Galien Paris Sud, UMR 8612, Univ Paris-Sud, CNRS, Université Paris-Saclay, 5 rue J.B. Clément, F-92290 Châtenay-Malabry, France; Lip(Sys)(2), Chimie Analytique Pharmaceutique (FKA EA4041 Groupe de Chimie Analytique de Paris-Sud), Univ Paris-Sud, Université Paris-Saclay, F-92290 Châtenay-Malabry, France.
authors.forename: Donia
authors.initials: D
authors.lastname: Essaid
authors.affiliation: Institut Galien Paris Sud, UMR 8612, Univ Paris-Sud, CNRS, Université Paris-Saclay, 5 rue J.B. Clément, F-92290 Châtenay-Malabry, France. Electronic address: veronique.rosilio@u-psud.fr.
authors.forename: Véronique
authors.initials: V
authors.lastname: Rosilio
authors.affiliation: Institut Galien Paris Sud, UMR 8612, Univ Paris-Sud, CNRS, Université Paris-Saclay, 5 rue J.B. Clément, F-92290 Châtenay-Malabry, France.
authors.forename: Katia
authors.initials: K
authors.lastname: Daghildjian
authors.affiliation: Institut Paris-Saclay d'Innovation Thérapeutique, UMS IPSIT SAMM, Châtenay-Malabry, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Solgadi
authors.affiliation: Institut Galien Paris Sud, UMR 8612, Univ Paris-Sud, CNRS, Université Paris-Saclay, 5 rue J.B. Clément, F-92290 Châtenay-Malabry, France.
authors.forename: Juliette
authors.initials: J
authors.lastname: Vergnaud
authors.affiliation: Lip(Sys)(2), Chimie Analytique Pharmaceutique (FKA EA4041 Groupe de Chimie Analytique de Paris-Sud), Univ Paris-Sud, Université Paris-Saclay, F-92290 Châtenay-Malabry, France.
authors.forename: Athena
authors.initials: A
authors.lastname: Kasselouri
authors.affiliation: Lip(Sys)(2), Chimie Analytique Pharmaceutique (FKA EA4041 Groupe de Chimie Analytique de Paris-Sud), Univ Paris-Sud, Université Paris-Saclay, F-92290 Châtenay-Malabry, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Chaminade
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry* / Q000378:metabolism; D002853:Chromatography, Liquid; D004847:Epithelial Cells / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008567:Membranes, Artificial; D018716:Molecular Mimicry; D010743:Phospholipids / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D055213:Retinal Pigment Epithelium / Q000737:chemistry / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010743:Phospholipids; D002784:Cholesterol
keywords: ARPE-19; High resolution mass spectrometry; Lipid monolayer; Lipidomics; Retinoblastoma; Y79