title: Ecotoxicological assessment of pyriproxyfen under environmentally realistic exposure conditions of integrated vector management for Aedes aegypti control in Brazil.
abstract: There is increasing concern to control Aedes aegypti mosquito exposure in developing countries such as Brazil. Thus, integrated approaches using a combination of chemical, pyriproxyfen larvicide, and biological, Xiphophorus maculatus, larvivorous fish species approaches are necessary and important to initiate more effective control against mosquito borne diseases. This study describes the toxicological effects of pyriproxyfen larvicide on the fish Xiphophorus maculatus, the larvivorous fish species employed to destroy A. aegypti larvae mosquito species. The toxicological profile of pyriproxyfen was evaluated to determine compatible concentrations for the use of this chemical in conjunction with X. maculatus as an integrated approach against A. aegypti mosquito larvae. According to the behavioral responses of fish, the no-observed-effect concentration (NOEC) and lowest-observed-effect concentration (LOEC) of pyriproxyfen were determined to be 2.5 and 5 µg/L, respectively. Bioassays indicated that although pyriproxyfen was not lethal to X. maculatus, the application of this compound at a concentration reported to control the emergence of A. aegypti larvae may decrease the swimming performance of larvivorous fish and their ability to ingest A. aegypti L4 larvae. Data show that integration of biological larvivorous fish and chemical larvicides is more effective when the appropriate larvicide concentration is utilized.
authors.affiliation: a Department of Environmental Health, Laboratory of Environmental Health , Federal University of Uberlândia, Santa Mônica Campus , Uberlândia , Minas Gerais , Brazil.
authors.forename: Evelyn Siqueira
authors.initials: ES
authors.lastname: Caixeta
authors.affiliation: a Department of Environmental Health, Laboratory of Environmental Health , Federal University of Uberlândia, Santa Mônica Campus , Uberlândia , Minas Gerais , Brazil.
authors.forename: Carolina Fabiano
authors.initials: CF
authors.lastname: Silva
authors.affiliation: b Institute of Genetics and Biochemistry, Department of Biotechnology , Federal University of Uberlândia, Umuarama Campus , Uberlândia , Minas Gerais , Brazil.
authors.forename: Vanessa Santana Vieira
authors.initials: VS
authors.lastname: Santos
authors.affiliation: c Department of Genetics and Biochemistry, Laboratory of Cytogenetics and Mutagenesis , Federal University of Uberlândia, Umuarama Campus , Uberlândia , Minas Gerais , Brazil.
authors.forename: Edimar
authors.initials: E
authors.lastname: Olegário de Campos Júnior
authors.affiliation: a Department of Environmental Health, Laboratory of Environmental Health , Federal University of Uberlândia, Santa Mônica Campus , Uberlândia , Minas Gerais , Brazil.
authors.forename: Boscolli Barbosa
authors.initials: BB
authors.lastname: Pereira
mesh_terms: D000330:Aedes / Q000254:growth & development; D000818:Animals; D001938:Brazil; D003532:Cyprinodontiformes; D004305:Dose-Response Relationship, Drug; D054750:Ecotoxicology; D004781:Environmental Exposure; D007303:Insect Vectors / Q000254:growth & development; D007306:Insecticides / Q000633:toxicity*; D007814:Larva / Q000254:growth & development; D008297:Male; D009032:Mosquito Control; D010572:Pest Control, Biological; D011725:Pyridines / Q000633:toxicity*
chemical_list: D007306:Insecticides; D011725:Pyridines; C055613:pyriproxyfen