title: High-effective cultivation of Halobacterium salinarum providing with bacteriorhodopsin production under controlled stress.
abstract: Submerged growth of Halobacterium salinarum and therefore synthesis of bacteriorhodopsin (BR) and carotenoids depend greatly on products of both chemical and/or photochemical oxidation of medium components and cellular metabolism which act as inhibitors. Some cultivation variants which allowed eliminating an adverse effect of inhibitors on biomass accumulation and BR synthesis are reviewed. The application of activated charcoal or ion exchange resin as adsorbents at preparing inoculums and the main cultivation stages was shown to allow controlling, namely lowering overstress of the halobacterial cells by metabolites. The halobacterial biomass containing BR up to 1,750mgL(-1) and the minimum amount of carotinoids that would BR greatly facilitate isolation was accumulated up to 45gL(-1) during eight-day cultivation with cell recycling through adsorbent suspension in a fed-batch mode. To control BR biosynthesis the express method of BR quantification based on colour shades of cell suspension was developed.
authors.affiliation: The Department of Biotechnology, Faculty of Biotechnology and Industrial Ecology, D.I. Mendeleyev University of Chemical Technology of Russia, 9 Miusskaya square, Moscow 125047, Russia. Electronic address: wsezart@yandex.ru.
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Kalenov
authors.affiliation: The Department of Biotechnology, Faculty of Biotechnology and Industrial Ecology, D.I. Mendeleyev University of Chemical Technology of Russia, 9 Miusskaya square, Moscow 125047, Russia.
authors.forename: Marina M
authors.initials: MM
authors.lastname: Baurina
authors.affiliation: The Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Skladnev
authors.affiliation: The Department of Biotechnology, Faculty of Biotechnology and Industrial Ecology, D.I. Mendeleyev University of Chemical Technology of Russia, 9 Miusskaya square, Moscow 125047, Russia.
authors.forename: Alexander Ye
authors.initials: AY
authors.lastname: Kuznetsov
mesh_terms: D001436:Bacteriorhodopsins / Q000235:genetics / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D019617:Halobacterium salinarum / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D001436:Bacteriorhodopsins
keywords: Bacteriorhodopsin; Carotinoids; Halobacterial metabolites; Halobacterium salinarum; Halophiles; High-cell density culture