title: 3D Imaging of Nanoparticle Distribution in Biological Tissue by Laser-Induced Breakdown Spectroscopy.
abstract: Nanomaterials represent a rapidly expanding area of research with huge potential for future medical applications. Nanotechnology indeed promises to revolutionize diagnostics, drug delivery, gene therapy, and many other areas of research. For any biological investigation involving nanomaterials, it is crucial to study the behavior of such nano-objects within tissues to evaluate both their efficacy and their toxicity. Here, we provide the first account of 3D label-free nanoparticle imaging at the entire-organ scale. The technology used is known as laser-induced breakdown spectroscopy (LIBS) and possesses several advantages such as speed of operation, ease of use and full compatibility with optical microscopy. We then used two different but complementary approaches to achieve 3D elemental imaging with LIBS: a volume reconstruction of a sliced organ and in-depth analysis. This proof-of-concept study demonstrates the quantitative imaging of both endogenous and exogenous elements within entire organs and paves the way for innumerable applications.
authors.affiliation: Institut Lumière Matière, UMR5306 Université de Lyon 1 - CNRS, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Gimenez
authors.affiliation: Institut Lumière Matière, UMR5306 Université de Lyon 1 - CNRS, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: B
authors.initials: B
authors.lastname: Busser
authors.affiliation: Institut Lumière Matière, UMR5306 Université de Lyon 1 - CNRS, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: F
authors.initials: F
authors.lastname: Trichard
authors.affiliation: Institut Lumière Matière, UMR5306 Université de Lyon 1 - CNRS, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Kulesza
authors.affiliation: Andor Technology, Springvale Business Park, Belfast BT12 7AL, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Laurent
authors.affiliation: CRITT Matériaux Alsace, 67305 Schiltigheim, France.
authors.forename: V
authors.initials: V
authors.lastname: Zaun
authors.affiliation: Institut Lumière Matière, UMR5306 Université de Lyon 1 - CNRS, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: F
authors.initials: F
authors.lastname: Lux
authors.affiliation: Institut Lumière Matière, UMR5306 Université de Lyon 1 - CNRS, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Benoit
authors.affiliation: Institut Lumière Matière, UMR5306 Université de Lyon 1 - CNRS, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: G
authors.initials: G
authors.lastname: Panczer
authors.affiliation: Institut Lumière Matière, UMR5306 Université de Lyon 1 - CNRS, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Dugourd
authors.affiliation: Institut Lumière Matière, UMR5306 Université de Lyon 1 - CNRS, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: O
authors.initials: O
authors.lastname: Tillement
authors.affiliation: CRITT Matériaux Alsace, 67305 Schiltigheim, France.
authors.forename: F
authors.initials: F
authors.lastname: Pelascini
authors.affiliation: Institut Lumière Matière, UMR5306 Université de Lyon 1 - CNRS, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: L
authors.initials: L
authors.lastname: Sancey
authors.affiliation: Institut Lumière Matière, UMR5306 Université de Lyon 1 - CNRS, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: V
authors.initials: V
authors.lastname: Motto-Ros
mesh_terms: D000818:Animals; D005260:Female; D021621:Imaging, Three-Dimensional; D007668:Kidney / Q000033:anatomy & histology*; D007834:Lasers; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*