title: Reticulocyte Preference and Stage Development of Plasmodium vivax Isolates.
abstract: Plasmodium vivax, the most widely distributed human malaria parasite, is restricted to reticulocytes, limiting its asexual proliferation. In recent years, cases of severe and high-level P. vivax parasitemia have been reported, challenging the assumption that all isolates are equally restricted. In this article, we analyze the reticulocyte preference of a large number of Indian P. vivax isolates. Our results show that P. vivax isolates significantly vary in their level of reticulocyte preference. In addition, by carefully staging the parasites, we find that P. vivax schizonts are largely missing in peripheral blood, with the presence of schizonts in circulation correlating with a high reticulocyte preference.
authors.affiliation: Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, Massachusetts.
authors.forename: Caeul
authors.initials: C
authors.lastname: Lim
authors.affiliation: Department of Chemistry, University of Washington, Seattle Department of Medicine, Goa Medical College and Hospital, Bambolim, India.
authors.forename: Ligia
authors.initials: L
authors.lastname: Pereira
authors.affiliation: Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, Massachusetts.
authors.forename: Kathryn Shaw
authors.initials: KS
authors.lastname: Saliba
authors.affiliation: Department of Chemistry, University of Washington, Seattle Department of Medicine, Goa Medical College and Hospital, Bambolim, India.
authors.forename: Anjali
authors.initials: A
authors.lastname: Mascarenhas
authors.affiliation: Department of Chemistry, University of Washington, Seattle.
authors.forename: Jennifer N
authors.initials: JN
authors.lastname: Maki
authors.affiliation: Department of Chemistry, University of Washington, Seattle.
authors.forename: Laura
authors.initials: L
authors.lastname: Chery
authors.affiliation: Department of Medicine, Goa Medical College and Hospital, Bambolim, India.
authors.forename: Edwin
authors.initials: E
authors.lastname: Gomes
authors.affiliation: Department of Chemistry, University of Washington, Seattle.
authors.forename: Pradipsinh K
authors.initials: PK
authors.lastname: Rathod
authors.affiliation: Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, Massachusetts.
authors.forename: Manoj T
authors.initials: MT
authors.lastname: Duraisingh
mesh_terms: D006801:Humans; D016780:Malaria, Vivax / Q000469:parasitology* / Q000473:pathology*; D010966:Plasmodium vivax / Q000502:physiology*; D012156:Reticulocytes / Q000469:parasitology*; D056189:Viral Tropism
keywords: Plasmodium vivax; asexual stage; reticulocyte preference