title: GABAergic Interneurons are Required for Generation of Slow CA1 Oscillation in Rat Hippocampus.
abstract: Neuronal oscillations are fundamental to hippocampal function. It has been shown that GABAergic interneurons make an important contribution to hippocampal oscillations, but the underlying mechanism is not well understood. Here, using whole-cell recording in the complete hippocampal formation isolated from rats at postnatal days 14-18, we showed that GABAA receptor-mediated activity enhanced the generation of slow CA1 oscillations. In vitro, slow oscillations (0.5-1.5 Hz) were generated in CA1 neurons, and they consisted primarily of excitatory rather than inhibitory membrane-potential changes. These oscillations were greatly reduced by blocking GABAA receptor-mediated activity with bicuculline and were enhanced by increasing such activity with midazolam, suggesting that interneurons are required for oscillation generation. Consistently, CA1 fast-spiking interneurons were found to generate action potentials usually preceding those in CA1 pyramidal cells. These findings indicate a GABAA receptor-based mechanism for the generation of the slow CA1 oscillation in the hippocampus.
authors.affiliation: Institute and Key Laboratory of Brain Functional Genomics of The Ministry of Education of China, Shanghai Key Laboratory of Brain Functional Genomics, School of Life Sciences, East China Normal University, Shanghai, 200062, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute and Key Laboratory of Brain Functional Genomics of The Ministry of Education of China, Shanghai Key Laboratory of Brain Functional Genomics, School of Life Sciences, East China Normal University, Shanghai, 200062, China.
authors.forename: Lidan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute and Key Laboratory of Brain Functional Genomics of The Ministry of Education of China, Shanghai Key Laboratory of Brain Functional Genomics, School of Life Sciences, East China Normal University, Shanghai, 200062, China.
authors.forename: Yu-Zhang
authors.initials: YZ
authors.lastname: Liu
authors.affiliation: Institute and Key Laboratory of Brain Functional Genomics of The Ministry of Education of China, Shanghai Key Laboratory of Brain Functional Genomics, School of Life Sciences, East China Normal University, Shanghai, 200062, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute and Key Laboratory of Brain Functional Genomics of The Ministry of Education of China, Shanghai Key Laboratory of Brain Functional Genomics, School of Life Sciences, East China Normal University, Shanghai, 200062, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Xue
authors.affiliation: Institute and Key Laboratory of Brain Functional Genomics of The Ministry of Education of China, Shanghai Key Laboratory of Brain Functional Genomics, School of Life Sciences, East China Normal University, Shanghai, 200062, China. zrwang@brain.ecnu.edu.cn.
authors.forename: Zhiru
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001640:Bicuculline / Q000494:pharmacology; D001683:Biological Clocks / Q000187:drug effects / Q000502:physiology*; D056547:CA1 Region, Hippocampal / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018757:GABA Modulators / Q000494:pharmacology; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D059330:GABAergic Neurons / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D007395:Interneurons / Q000187:drug effects / Q000502:physiology*; D008874:Midazolam / Q000494:pharmacology; D009025:Morpholines / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C095804:(+)-(S)-5,5-dimethylmorpholinyl-2-acetic acid; D018691:Excitatory Amino Acid Antagonists; D018757:GABA Modulators; D058787:GABA-A Receptor Antagonists; D009025:Morpholines; D011810:Quinoxalines; C056723:FG 9041; D008874:Midazolam; D001640:Bicuculline
keywords: GABAA receptor; GABAergic interneuron; Hippocampus; Oscillation; Synchronization