title: Comparison of the immunogenicity of two inactivated recombinant rabies viruses overexpressing the glycoprotein.
abstract: Two recombinant rabies viruses overexpressing their glycoprotein (G) were compared in this study, with the overexpressed G inserted between P and M genes (named LBNSE-PM-G), and between the G and L genes (named LBNSE-GL-G), respectively. LBNSE-PM-G produced more G protein and induced stronger apoptosis than LBNSE-GL-G in infected cells, while the amount of virion-incorporated G in LBNSE-PM-G was less than in LBNSE-GL-G. Mice immunized with inactivated LBNSE-PM-G produced lower titers of virus-neutralizing antibody, and this recombinant conferred worse protection than LBNSE-GL-G. Our results suggest that over expressed G gene inserted between G and L, but not between P and M, enhanced the immunogenicity when used as an inactivated rabies vaccine.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Muhammad Tariq
authors.initials: MT
authors.lastname: Navid
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Min
authors.initials: M
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.|Department of Pathology, University of Georgia, Athens, GA, 30602, USA.
authors.forename: Zhen F
authors.initials: ZF
authors.lastname: Fu
authors.affiliation: Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Academy of Preventive Medicine, Wuhan, 430070, China. 741568159@qq.com.
authors.forename: Lijun
authors.initials: L
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China. lingzhao@mail.hzau.edu.cn.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. lingzhao@mail.hzau.edu.cn.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology*; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D011818:Rabies / Q000517:prevention & control; D011819:Rabies Vaccines / Q000235:genetics / Q000276:immunology*; D011820:Rabies virus / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D011819:Rabies Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus
keywords: Inactivated vaccine; Over-expressing glycoprotein; Rabies virus; Virus-neutralizing antibody