title: Precut cornea for Descemet's stripping endothelial keratoplasty: experience at a single eye bank.
abstract: The aim of the study is to describe the experience with precut facility for endothelial keratoplasty at a single eye bank affiliated to a tertiary eye care center in India. Data on precut tissues from Nov 2012 to Dec 2014 were retrospectively reviewed from the electronic database of the eye bank of a tertiary eye care center in South India. Donor characteristic data including donor age, precut and postcut endothelial cell density (ECD), recipient age, and thickness of graft were collected. The number of precuts increased from 42 in 2012 (Nov and Dec) and 422 in 2013 to 584 in 2014. Of the total of 1048 precuts, seven (0.67 %) were miscut and could not be utilized for transplants. The donor age ranged from 2 to 89 years. A mean change of 43.6 ± 325.2 cells/mm2in ECD was noted after cut, proportional increase in mean being 1.9 %. The change in ECD after cut was negatively correlated with ECD before cut. The recipient age ranged from 1 to 89 years. The median thickness of donor lenticule after cut was 148 µm (interquartile range 131-166 µm). Analysis of precut donor corneas from a single eye bank shows that the ECD of the processed tissues was excellent for transplantation. The tissue wastage in the hands of eye bank personal was minimal.
authors.affiliation: Ophthalmic Biophysics, L V Prasad Eye Institute, Hyderabad, India.
authors.forename: Ashik
authors.initials: A
authors.lastname: Mohamed
authors.affiliation: Tej Kohli Cornea Institute, L V Prasad Eye Institute, L V Prasad Marg, Banjara Hills, Hyderabad, Telangana, 500034, India. sunita@lvpei.org.|Ramayamma International Eye Bank, L V Prasad Eye Institute, Hyderabad, India. sunita@lvpei.org.
authors.forename: Sunita
authors.initials: S
authors.lastname: Chaurasia
authors.affiliation: Ramayamma International Eye Bank, L V Prasad Eye Institute, Hyderabad, India.
authors.forename: Venkataswamy
authors.initials: V
authors.lastname: Chandragiri
authors.affiliation: Ramayamma International Eye Bank, L V Prasad Eye Institute, Hyderabad, India.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Kandhibanda
authors.affiliation: Ramayamma International Eye Bank, L V Prasad Eye Institute, Hyderabad, India.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Gunnam
authors.affiliation: Tej Kohli Cornea Institute, L V Prasad Eye Institute, L V Prasad Marg, Banjara Hills, Hyderabad, Telangana, 500034, India.|Ramayamma International Eye Bank, L V Prasad Eye Institute, Hyderabad, India.
authors.forename: Prashant
authors.initials: P
authors.lastname: Garg
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count; D002648:Child; D002675:Child, Preschool; D003315:Cornea / Q000601:surgery*; D003316:Corneal Diseases / Q000601:surgery*; D057111:Descemet Stripping Endothelial Keratoplasty / Q000379:methods*; D004728:Endothelium, Corneal / Q000473:pathology; D005125:Eye Banks; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007223:Infant; D008297:Male; D033401:Microscopy, Acoustic; D008875:Middle Aged; D012189:Retrospective Studies; D013048:Specimen Handling / Q000379:methods; D014019:Tissue Donors; D016896:Treatment Outcome; D014792:Visual Acuity; D055815:Young Adult
keywords: Endothelial cell density; Eye banking; Keratoplasty; Precut cornea