title: One-pot synthesis of quantum dot-labeled hydrophilic molecularly imprinted polymer nanoparticles for direct optosensing of folic acid in real, undiluted biological samples.
abstract: A facile and efficient one-pot approach for the synthesis of quantum dot (QD)-labeled hydrophilic molecularly imprinted polymer (MIP) nanoparticles for direct optosensing of folic acid (FA) in the undiluted bovine and porcine serums is described. Hydrophilic macromolecular chain transfer agent-mediated reversible addition-fragmentation chain transfer (RAFT) precipitation polymerization was used to implement the molecular imprinting of FA in the presence of CdTe quantum dots (QDs). The resulting FA-imprinted polymer nanoparticles with surface-grafted hydrophilic poly(glyceryl monomethacrylate) brushes and QDs labeling not only showed outstanding specific molecular recognition toward FA in biological samples, but also exhibited good photostability, rapid binding kinetics, and obvious template binding-induced fluorescence quenching. These characteristics make them a useful fluorescent chemosensor for directly and selectively optosensing FA in the undiluted bovine and porcine serums, with its limit of detection being 0.025μM and average recoveries ranging from 98% to 102%, even in the presence of several interfering compounds. This advanced fluorescent MIP chemosensor is highly promising for rapid quantification of FA in such applications as clinical diagnostics and food analysis.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials (Ministry of Education), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and College of Chemistry, Nankai University, Tianjin, 300071 PR China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials (Ministry of Education), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and College of Chemistry, Nankai University, Tianjin, 300071 PR China.
authors.forename: Zhengzheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials (Ministry of Education), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and College of Chemistry, Nankai University, Tianjin, 300071 PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials (Ministry of Education), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and College of Chemistry, Nankai University, Tianjin, 300071 PR China. Electronic address: zhanghuiqi@nankai.edu.cn.
authors.forename: Huiqi
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000295:instrumentation; D019187:Cadmium Compounds / Q000138:chemical synthesis*; D002417:Cattle; D005492:Folic Acid / Q000097:blood*; D057927:Hydrophobic and Hydrophilic Interactions; D057054:Molecular Imaging / Q000295:instrumentation*; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013194:Staining and Labeling; D013552:Swine; D013691:Tellurium
chemical_list: D019187:Cadmium Compounds; D011108:Polymers; D005492:Folic Acid; D013691:Tellurium; C028337:cadmium telluride
keywords: Biological samples; Chemosensor; Fluorescence quenching; Molecularly imprinted polymers; Quantum dots