title: NPM1, FLT3 and CEBPA mutations in pediatric patients with AML from Argentina: incidence and prognostic value.
abstract: Mutations in NPM1, FLT3 and CEBPA genes are found in 25-35 % of adult acute myeloblastic leukemia (AML) cases and correlate with prognosis. To date, there have been no reports about these mutations in pediatric AML from Argentina. The aims of the present study were to describe the incidence of NPM1, FLT3 and CEBPA mutations and to analyze their prognostic impact in this population. The incidences of these mutations within a population of 216 pediatric AML cases were: NPM1-mutated 4.2 %, CEBPA-mutated 1.9 %, FLT3-ITD 10.2 % and FLT3-TKD 7.9 %. Among 33 patients with normal karyotype, we found significantly higher frequencies for NPM1-mutated 24.2 % and CEBPA-mutated 12.1 %. Overall survival (pOS) for the 163 eligible non-acute promyelocytic leukemia cases was 46.2 ± 4.3 %, while leukemia-free survival probability was 51.0 ± 4.4 % (n = 135). The NPM1-mutated/FLT3-ITD-negative genotype showed better outcome than any other combined NPM1/FLT3 genotype; this difference was statistically significant within the group of high-risk patients (pOS ± SE 83.3 ± 15.2 % versus 33.1 ± 4.7 %; p = 0.0251). This is the first report of the frequencies of these mutations in Argentina. Despite the limited number of patients, a favorable prognosis of AML with genotype NPM1-mutated/FLT3-ITD-negative was confirmed. This is especially relevant within the high-risk group of patients, as it may contribute to the detection of patients with better prognosis, and thus avoid unnecessary treatment intensification.
authors.affiliation: Department of Hematology and Oncology, Hospital de Pediatría Prof. Dr. J. P. Garrahan, Combate de los Pozos 1881, 1245, Buenos Aires, Argentina. patrirubio13@gmail.com.
authors.forename: Patricia
authors.initials: P
authors.lastname: Rubio
authors.affiliation: Department of Hematology and Oncology, Hospital de Pediatría Prof. Dr. J. P. Garrahan, Combate de los Pozos 1881, 1245, Buenos Aires, Argentina.
authors.forename: B
authors.initials: B
authors.lastname: Campos
authors.affiliation: Department of Hematology and Oncology, Hospital de Pediatría Prof. Dr. J. P. Garrahan, Combate de los Pozos 1881, 1245, Buenos Aires, Argentina.
authors.forename: J A
authors.initials: JA
authors.lastname: Digiorge
authors.affiliation: Department of Genetics, Hospital de Pediatría Prof. Dr. J. P. Garrahan, Buenos Aires, Argentina.
authors.forename: M S
authors.initials: MS
authors.lastname: Gallego
authors.affiliation: Department of Hematology and Oncology, Hospital de Pediatría Prof. Dr. J. P. Garrahan, Combate de los Pozos 1881, 1245, Buenos Aires, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Medina
authors.affiliation: Department of Immunology and Rheumatology, Hospital de Pediatría Prof. Dr. J. P. Garrahan, Buenos Aires, Argentina.
authors.forename: J G
authors.initials: JG
authors.lastname: Rossi
authors.affiliation: Department of Hematology and Oncology, Hospital de Pediatría Prof. Dr. J. P. Garrahan, Combate de los Pozos 1881, 1245, Buenos Aires, Argentina.
authors.forename: M S
authors.initials: MS
authors.lastname: Felice
authors.affiliation: Department of Hematology and Oncology, Hospital de Pediatría Prof. Dr. J. P. Garrahan, Combate de los Pozos 1881, 1245, Buenos Aires, Argentina.
authors.forename: C N
authors.initials: CN
authors.lastname: Alonso
mesh_terms: D000293:Adolescent; D001118:Argentina / Q000453:epidemiology; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D015994:Incidence; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics* / Q000401:mortality; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D000090243:Nucleophosmin; D011379:Prognosis; D016019:Survival Analysis; D020080:Tandem Repeat Sequences; D051941:fms-Like Tyrosine Kinase 3 / Q000235:genetics*
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C515848:CEBPA protein, human; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D000090243:Nucleophosmin; C497970:FLT3 protein, human; D051941:fms-Like Tyrosine Kinase 3
keywords: AML; CEBPA; Childhood; FLT3; NPM1; Prognostic