title: A New Method for the Isolation of Ergosterol and Peroxyergosterol as Active Compounds of Hygrophoropsis aurantiaca and in Vitro Antiproliferative Activity of Isolated Ergosterol Peroxide.
abstract: In the present study, ergosterol peroxide and ergosterol were isolated for the first time from fresh fruit bodies of Hygrophoropsis aurantiaca (False Chanterelle). The substances were characterized mainly by spectroscopic methods (¹H-NMR, (13)C-NMR, DEPT-45, DEPT-90, DEPT-135, 2D-NMR). In our study, a new specific thin layer chromatographic method was developed for determination of ergosterol and ergosterol peroxide in H. aurantiaca extract. The method is based on the separation of n-hexane extract on silica gel (Silica Gel G) TLC plates using the optimized solvent system toluene/ethyl acetate (3:1; v/v). The main advantages of the developed method are the simplicity of operation and the low cost. The in vitro study results revealed the antiproliferative properties of ergosterol peroxide against LS180 human colon cancer cells. The described effect was attributed both to altered mitochondrial activity and decreased DNA synthesis. Additionally, in the same concentration range the investigated compound was not toxic to CCD 841 CoTr human colon epithelial cells. The present study suggests that fruit bodies of H. aurantiaca have great potential for producing substances and extracts with potential applications in medicine.
authors.affiliation: Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chod&#378;ki Str., 20-093 Lublin, Poland. renatanowak@umlub.pl.
authors.forename: Renata
authors.initials: R
authors.lastname: Nowak
authors.affiliation: Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chod&#378;ki Str., 20-093 Lublin, Poland. medea_ua@tlen.pl.
authors.forename: Marta
authors.initials: M
authors.lastname: Drozd
authors.affiliation: Analytical Laboratory, Faculty of Chemistry, University of Maria Curie-Sklodowska, 3/27 M.C. Sk&#322;odowska Sq., 20-031 Lublin, Poland. emendyk@umcs.pl.
authors.forename: Ewaryst
authors.initials: E
authors.lastname: Mendyk
authors.affiliation: Department of Medical Biology, Institute of Rural Health, 2 Jaczewskiego Str., 20-090 Lublin, Poland. martalemieszek@gmail.com.
authors.forename: Marta
authors.initials: M
authors.lastname: Lemieszek
authors.affiliation: Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chod&#378;ki Str., 20-093 Lublin, Poland. ola.krakowiak@wp.pl.
authors.forename: Olga
authors.initials: O
authors.lastname: Krakowiak
authors.affiliation: Department of Phytochemistry, Institute of Pharmacology, Polish Academy of Sciences, 12 Smętna Str., 31-343 Krak&#243;w, Poland. kisielw@if-pan.krakow.pl.
authors.forename: Wanda
authors.initials: W
authors.lastname: Kisiel
authors.affiliation: Department of Medical Biology, Institute of Rural Health, 2 Jaczewskiego Str., 20-090 Lublin, Poland. rzeski.wojciech@imw.lublin.pl.|Department of Virology and Immunology, Institute of Microbiology and Biotechnology, University of Maria Curie-Sklodowska, 19 Akademicka Str., 20-033 Lublin, Poland. rzeski.wojciech@imw.lublin.pl.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Rzeski
authors.affiliation: Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chod&#378;ki Str., 20-093 Lublin, Poland. k.szewczyk@umlub.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Szewczyk
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002853:Chromatography, Liquid; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D015394:Molecular Structure; D013057:Spectrum Analysis
chemical_list: D000970:Antineoplastic Agents; C036071:ergosterol-5,8-peroxide; D004875:Ergosterol
keywords: Hygrophoropsis aurantiaca; TLC-DPPH; antiproliferative effect; cytotoxicity; ergosterol; peroxyergosterol