title: Microbial metabolism of thiopurines: A method to measure thioguanine nucleotides.
abstract: Thiopurines are anti-inflammatory prodrugs. We hypothesised that bacteria may contribute to conversion to active drug. Escherichia coli strain DH5α was evaluated to determine whether it could metabolise the thiopurine drugs, thioguanine or mercaptopurine, to thioguanine nucleotides. A rapid and reliable high performance liquid chromatography (ultraviolet detection) method was developed to quantify indirectly thioguanine nucleotides, by measuring thioguanine nucleoside.
authors.affiliation: Immunity Infection and Inflammation Program, Mater Research Institute-University of Queensland, Brisbane, Queensland 4101, Australia; Translational Research Institute, 37 Kent Street, Woolloongabba, Queensland 4102, Australia; School of Pharmacy, Griffith University, Queensland 4125, Australia. Electronic address: ramya.movva@mater.uq.edu.au.
authors.forename: Ramya
authors.initials: R
authors.lastname: Movva
authors.affiliation: Inflammatory Diseases Biology & Therapeutics Program, Mater Research Institute-University of Queensland, Brisbane, Queensland 4101, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: Lobb
authors.affiliation: Translational Research Institute, 37 Kent Street, Woolloongabba, Queensland 4102, Australia; The University of Queensland Diamantina Institute, Brisbane, Queensland 4102, Australia.
authors.forename: Páraic
authors.initials: P
authors.lastname: Ó Cuív
authors.affiliation: Immunity Infection and Inflammation Program, Mater Research Institute-University of Queensland, Brisbane, Queensland 4101, Australia; Translational Research Institute, 37 Kent Street, Woolloongabba, Queensland 4102, Australia; School of Medicine-University of Queensland, St Lucia, Queensland 4072, Australia.
authors.forename: Timothy H J
authors.initials: THJ
authors.lastname: Florin
authors.affiliation: Inflammatory Diseases Biology & Therapeutics Program, Mater Research Institute-University of Queensland, Brisbane, Queensland 4101, Australia; School of Pharmacy, University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: John A
authors.initials: JA
authors.lastname: Duley
authors.affiliation: Immunity Infection and Inflammation Program, Mater Research Institute-University of Queensland, Brisbane, Queensland 4101, Australia; Translational Research Institute, 37 Kent Street, Woolloongabba, Queensland 4102, Australia; School of Medicine-University of Queensland, St Lucia, Queensland 4072, Australia. Electronic address: iulia.oancea@mater.uq.edu.au.
authors.forename: Iulia
authors.initials: I
authors.lastname: Oancea
mesh_terms: D000893:Anti-Inflammatory Agents / Q000378:metabolism*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000378:metabolism; D015122:Mercaptopurine / Q000378:metabolism; D009705:Nucleosides / Q000032:analysis; D009711:Nucleotides / Q000032:analysis; D013866:Thioguanine / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D009705:Nucleosides; D009711:Nucleotides; D015122:Mercaptopurine; D013866:Thioguanine
keywords: Bacteria; Inflammatory bowel disease; Mercaptopurine; Thioguanine; Thioguanosine