title: Ameliorative effect of dietary genistein on diabetes induced hyper-inflammation and oxidative stress during early stage of wound healing in alloxan induced diabetic mice.
abstract: Among the diabetic complications, diabetic foot ulcer due to delayed wound healing is one of the most significant clinical problems. Early inflammatory stage is important for better prognosis during wound healing. Thus, regulation of inflammatory response during early stage of wound healing is main target for complete cutaneous recovery. This study investigated the role of genistein supplementation in inflammation and oxidative stress, which are related to NLRP3 inflammasome, NFκB and Nrf2 activation, during cutaneous wound healing in alloxan-induced diabetic mice. Mice with diabetes with fasting blood glucose (FBG) levels > 250 mg/dl were fed diets with AIN-93G rodent diet containing 0%, 0.025% (LG) or 0.1% (HG) genistein. After 2 weeks of genistein supplementation, excisional wounds were made by biopsy punches (4 mm). Genistein supplementation improved fasting glucose levels and wound closure rate. Moreover, genistein supplementation restored NLRP3 inflammasome (NLRP3, ASC and caspase-1) at the basal level and ameliorated both inflammation (TNFα, iNOS, COX2 and NFκB) and antioxidant defense system (Nrf2, HO-1, GPx, and catalase) during early stage of wound healing in diabetic mice. Taken together, genistein supplementation would be a potential therapeutic nutrient in prevention and treatment of delayed wound healing by modulation of inflammation and oxidative stress during inflammatory stage.
authors.affiliation: Department of Food and Nutrition, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Hyeyoon
authors.initials: H
authors.lastname: Eo
authors.affiliation: Department of Food and Nutrition, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Hea-Ji
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Kyung Hee University, Seoul, Republic of Korea. Electronic address: ylim@khu.ac.kr.
authors.forename: Yunsook
authors.initials: Y
authors.lastname: Lim
mesh_terms: D000496:Alloxan; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications* / Q000188:drug therapy*; D019587:Dietary Supplements; D005215:Fasting / Q000097:blood; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D019833:Genistein / Q000494:pharmacology / Q000627:therapeutic use*; D058847:Inflammasomes / Q000378:metabolism; D007249:Inflammation / Q000150:complications / Q000188:drug therapy* / Q000473:pathology*; D008813:Mice, Inbred ICR; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D012867:Skin / Q000187:drug effects / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse; D000496:Alloxan; D019833:Genistein
keywords: Delayed wound healing; Diabetes; Genistein; NFκB; NLRP3 inflammasome; Nrf2