title: An Immuno-Magnetic Nanobead Probe Competitive Assay for Rapid Detection of Salmonella choleraesuis.
abstract: A competitive lateral flow assay for the rapid detection of Salmonella choleraesuis was developed. Immuno-magnetic nanobeads were produced by covalently coupling anti-Salmonella choleraesuis antibody to magnetic nanobeads. These immuno-magnetic nanobeads were used as visually detected probes in the subsequent assay. Compared with the traditional sandwich assay, which is used for detecting macro-molecules, this new method was developed based on the competitive relationship between S. choleraesuis in the inspected sample and the outer membrane protein immobilized on the T line. Thus, only one antibody was necessary in the new assay, whereas a pair of rigorously selected antibodies were required in the sandwich assay. The sensitivity of the competitive assay for S. choleraesuis was 1.2 x 10(7) cfu/mL. In addition, no cross reactions were found in the 17 common non-Salmonella bacteria strains and in the 4 Salmonella strains of other serotypes. Thus, with satisfactory sensitivity and specificity, the assay can be applied for the rapid detection of pre-enriched culture that may contain S. choleraesuis.
authors.forename: Daofeng
authors.initials: D
authors.lastname: Liu
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Yu
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Huang
authors.forename: Shuying
authors.initials: S
authors.lastname: Wang
authors.forename: Jingyun
authors.initials: J
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Guo
authors.forename: Chaolian
authors.initials: C
authors.lastname: Xu
authors.forename: Shiqi
authors.initials: S
authors.lastname: Xia
authors.forename: Weihua
authors.initials: W
authors.lastname: Lai
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D018189:Immunomagnetic Separation; D015335:Molecular Probes; D036103:Nanotechnology; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D015335:Molecular Probes