title: Immune Cell Isolation from Mouse Femur Bone Marrow.
abstract: The bone marrow is the site of hematopoesis and contains mixed population of blood cells including erythrocytes, granulocytes, monocytes, dendritic cells, lymphocytes and hematopoietic stem cells. The following protocol provides a simple and fast method for isolation of bone marrow immune cells (no erythrocytes) from mouse femurs with a yield of approximate 8 × 107cells in 5 ml culture media (1.6 × 104cells/μl). Further isolation or flow cytometric analysis might be required for study of specific immune cell types.
authors.affiliation: Division of Biosciences, The Ohio State University, Columbus, USA; Institute for Behavioral Medicine Research, The Ohio State University Wexner Medical Center, Columbus, USA.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Division of Biosciences, The Ohio State University, Columbus, USA; Institute for Behavioral Medicine Research, The Ohio State University Wexner Medical Center, Columbus, USA.
authors.forename: Ning
authors.initials: N
authors.lastname: Quan