title: Quantitative, Point-of-Care Immunoassay Platform to Guide and Monitor Sickle Cell Disease Therapy.
abstract: Sickle cell patients often require monthly transfusions with normal blood to treat the many complications of the disease. In this therapy, the clinician lowers the amount of hemoglobin S (HbS) containing red blood cells (RBCs) by transfusing normal blood units containing hemoglobin A (HbA). We have developed a point-of-care (POC) quantitative immunoassay for HbS to serve as a diagnostic aid for clinicians providing this life-saving treatment. The test consists of a small-footprint reader and cartridges that quantify the percentage of HbS in a small volume of patient blood. The test reports % HbS values in the range from 5 to 86% that highly correlate (slope 1.03, R(2) = 0.97) with currently used central laboratory HPLC systems. The test also shows a 1% limit of blank, 2% limit of detection, and 5% limit of quantitation. The test was also shown to encounter minimal effects from potential interferences. This cost-effective, POC HbS quantitative approach will allow for real-time transfusion monitoring in sickle cell treatment settings and therefore improve workflow and allow clinicians to quickly make informed therapeutic decisions.
authors.affiliation: BioMedomics Inc. , Durham, North Carolina 27709, United States.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Yang
authors.affiliation: BioMedomics Inc. , Durham, North Carolina 27709, United States.
authors.forename: Hunter D
authors.initials: HD
authors.lastname: Reavis
authors.affiliation: BioMedomics Inc. , Durham, North Carolina 27709, United States.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Roberts
authors.affiliation: BioMedomics Inc. , Durham, North Carolina 27709, United States.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000755:Anemia, Sickle Cell / Q000175:diagnosis / Q000188:drug therapy*; D004912:Erythrocytes / Q000737:chemistry*; D006451:Hemoglobin, Sickle / Q000032:analysis*; D006801:Humans; D007118:Immunoassay; D019095:Point-of-Care Systems
chemical_list: D006451:Hemoglobin, Sickle