title: Normal endometrial stromal cells regulate 17β-estradiol-induced epithelial-mesenchymal transition via slug and E-cadherin in endometrial adenocarcinoma cells in vitro.
abstract: Stroma-tumor communication participates in the pathogenesis of endometrial carcinomas. In previous studies, we found that normal stromal cells inhibited the growth of endometrial carcinoma cells. Here, we investigated the role of normal stromal cells in the epithelial-mesenchymal transition (EMT) of endometrial carcinoma cells and explored the possible mechanism implied. We found that conditioned medium (CM) by normal endometrial stromal cells (NSC) reduced cell growth and induced cell apoptosis in Ishikawa cells. CM by NSC inhibited 17β-estradiol-induced cell growth and apoptosis decrease in Ishikawa cells. Moreover, CM by NSC inhibited the migration and invasion, and 17β-estradiol-induced migration and invasion in Ishikawa cells. Meanwhile, CM by NSC decreased Slug expression and 17β-estradiol-induced Slug expression, increased E-cadherin expression and abolished 17β-estradiol-induced E-cadherin reduction in Ishikawa cells. In conclusion, normal stromal factors can inhibit 17β-estradiol-induced cell proliferation and apoptosis inhibition, and abolished 17β-estradiol-induced EMT in endometrial cancer cell via regulating E-cadherin and Slug expression.
authors.affiliation: a Department of Obstetrics and Gynecology and.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a Department of Obstetrics and Gynecology and.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: a Department of Obstetrics and Gynecology and.
authors.forename: Shasha
authors.initials: S
authors.lastname: Qi
authors.affiliation: b Department of Urology , Shandong Provincial Hospital Affiliated to Shandong University , Jinan , Shandong , China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Liu
authors.affiliation: a Department of Obstetrics and Gynecology and.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Fu
authors.affiliation: a Department of Obstetrics and Gynecology and.
authors.forename: Mingjiang
authors.initials: M
authors.lastname: Li
authors.affiliation: a Department of Obstetrics and Gynecology and.
authors.forename: Xingbo
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D000328:Adult; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism*; D045744:Cell Line, Tumor; D016889:Endometrial Neoplasms / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006801:Humans; D000071250:Snail Family Transcription Factors / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D004958:Estradiol
keywords: 17β-estradiol; endometrial adenocarcinoma; epithelial-mesenchymal transition; slug/E-cadherin; stroma-tumor communication