title: Comparative Transcriptome Analysis Reveals Heat-Responsive Genes in Chinese Cabbage (Brassica rapa ssp. chinensis).
abstract: Chinese cabbage (Brassica rapa ssp. chinensis) is an economically and agriculturally significant vegetable crop and is extensively cultivated throughout the world. Heat stress disturbs cellular homeostasis and causes visible growth inhibition of shoots and roots, severe retardation in growth and development, and even death. However, there are few studies on the transcriptome profiling of heat stress in non-heading Chinese cabbage. In this study, we investigated the transcript profiles of non-heading Chinese cabbage from heat-sensitive and heat-tolerant varieties "GHA" and "XK," respectively, in response to high temperature using RNA sequencing (RNA seq). Approximately 625 genes were differentially expressed between the two varieties. The responsive genes can be divided into three phases along with the time of heat treatment: response to stimulus, programmed cell death and ribosome biogenesis. Differentially expressed genes (DEGs) were identified in the two varieties, including transcription factors (TFs), kinases/phosphatases, genes related to photosynthesis and effectors of homeostasis. Many TFs were involved in the heat stress response of Chinese cabbage, including NAC069 TF which was up-regulated at all the heat treatment stages. And their expression levels were also validated by quantitative real-time-PCR (qRT-PCR). These candidate genes will provide genetic resources for further improving the heat-tolerant characteristics in non-heading Chinese cabbage.
authors.affiliation: Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science and Technology Wuhan, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of life Sciences, Wuhan UniversityWuhan, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesWuhan, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Hu
authors.affiliation: Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science and Technology Wuhan, China.
authors.forename: Xingxue
authors.initials: X
authors.lastname: Huang
authors.affiliation: Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science and Technology Wuhan, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science and Technology Wuhan, China.
authors.forename: Guolin
authors.initials: G
authors.lastname: Zhou
authors.affiliation: BGI-Shenzhen Shenzhen, China.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Yan
keywords: Chinese cabbage; NAC; RNA-seq; heat stress; transcriptome