title: Detection of Silver Ions Using Dielectrophoretic Tweezers-Based Force Spectroscopy.
abstract: Understanding of the interactions of silver ions (Ag+) with polynucleotides is important not only to detect Ag+over a wide range of concentrations in a simple, robust, and high-throughput manner but also to investigate the intermolecular interactions of hydrogen and coordinate interactions that are generated due to the interplay of Ag+, hydrogen ions (H+), and polynucleotides since it is critical to prevent adverse environmental effects that may cause DNA damage and develop strategies to treat this damage. Here, we demonstrate a novel approach to simultaneously detect Ag+satisfying the above requirements and examine the combined intermolecular interactions of Ag+-polycytosine and H+-polycytosine DNA complexes using dielectrophoretic tweezers-based force spectroscopy. For this investigation, we detected Ag+over a range of concentrations (1 nM to 100 μM) by quantifying the rupture force of the combined interactions and examined the interplay between the three factors (Ag+, H+, and polycytosine) using the same assay for the detection of Ag+. Our study provides a new avenue not only for the detection of heavy metal ions but also for the investigation of heavy metal ions-polynucleotide DNA complexes using the same assay.
authors.affiliation: Department of Biomedical Engineering, Yonsei University , Wonju 26493, Republic of Korea.
authors.forename: Seungyeop
authors.initials: S
authors.lastname: Choi
authors.affiliation: Department of Biomedical Engineering, Yonsei University , Wonju 26493, Republic of Korea.|School of Public Health, Harvard University , Boston, Massachusetts 02115, United States.
authors.forename: Gyudo
authors.initials: G
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, Yonsei University , Wonju 26493, Republic of Korea.
authors.forename: In Soo
authors.initials: IS
authors.lastname: Park
authors.affiliation: Department of Biomedical Engineering, Yonsei University , Wonju 26493, Republic of Korea.
authors.forename: Myeonggu
authors.initials: M
authors.lastname: Son
authors.affiliation: Department of Control and Instrumentation Engineering, Korea University , Sejong 30019, Republic of Korea.
authors.forename: Woong
authors.initials: W
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, Yonsei University , Wonju 26493, Republic of Korea.
authors.forename: Hyungbeen
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, Yonsei University , Wonju 26493, Republic of Korea.
authors.forename: Sei-Young
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Mechanical Engineering, Korea University , Seoul 02841, Republic of Korea.
authors.forename: Sungsoo
authors.initials: S
authors.lastname: Na
authors.affiliation: Department of Bio-convergence Engineering, Korea University , Seoul 02841, Republic of Korea.
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Yoon
authors.affiliation: Department of Bioengineering, University Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
authors.forename: Rashid
authors.initials: R
authors.lastname: Bashir
authors.affiliation: Department of Control and Instrumentation Engineering, Korea University , Sejong 30019, Republic of Korea.
authors.forename: Jinsung
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Biomedical Engineering, Yonsei University , Wonju 26493, Republic of Korea.
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Lee