title: Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species.
abstract: OBJECTIVE
  Nuclear microsatellites (nSSRs) were designed for Guibourtia tessmannii (Fabaceae, Caesalpinioideae), a highly exploited African timber tree, to study population genetic structure and gene flow.
  
  
  RESULTS
  We developed 16 polymorphic nSSRs from a genomic library tested in three populations of G. tessmannii and two populations of G. coleosperma. These nSSRs display three to 14 alleles per locus (mean 8.94) in G. tessmannii. Cross-amplification tests in nine congeneric species demonstrated that the genus Guibourtia contains diploid and polyploid species. Flow cytometry results combined with nSSR profiles suggest that G. tessmannii is octoploid.
  
  
  CONCLUSIONS
  nSSRs revealed that African Guibourtia species include both diploid and polyploid species. These markers will provide information on the mating system, patterns of gene flow, and genetic structure of African Guibourtia species.
authors.affiliation: Tropical Forestry, Management of Forest Resources, TERRA, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, Belgium.
authors.forename: Felicien
authors.initials: F
authors.lastname: Tosso
authors.affiliation: Tropical Forestry, Management of Forest Resources, TERRA, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, Belgium.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Doucet
authors.affiliation: Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, B-1050 Brussels, Belgium.
authors.forename: Esra
authors.initials: E
authors.lastname: Kaymak
authors.affiliation: Tropical Forestry, Management of Forest Resources, TERRA, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, Belgium; Nature+ asbl, Rue Provinciale 62, 1301 Wavre, Belgium.
authors.forename: Kasso
authors.initials: K
authors.lastname: Daïnou
authors.affiliation: Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, B-1050 Brussels, Belgium; Bioversity International, Forest Genetic Resources Programme, Sub-Regional Office for Central Africa, P.O. Box 2008, Messa, Yaoundé, Cameroon; Institut de Recherche pour le Développement, UMR-DIADE, BP 64501, 34394 Montpellier, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Duminil
authors.affiliation: Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, B-1050 Brussels, Belgium.
authors.forename: Olivier J
authors.initials: OJ
authors.lastname: Hardy
keywords: Fabaceae; Guibourtia; flow cytometry; microsatellites; next-generation sequencing; polyploidy