title: Characterization of durum wheat high molecular weight glutenin subunits Bx20 and By20 sequences by a molecular and proteomic approach.
abstract: Wheat high molecular weight glutenin subunit variation is important because of its great influence on glutenin polymer structure, that is related to dough technological properties. Among the different subunits, the pair Bx20 and By20 is known to have a negative effect on quality, but the reasons are not clear: Bx20 has two cysteines, which theoretically make this subunit a chain extender of the glutenin polymer, just like the other Bx subunits, showing four cysteines, two of which should be involved in intra-molecular disulfide bonds. By20 has never been characterized so far at molecular level. Here we report the nucleotide sequences of Bx20 and By20 genes isolated from the durum wheat cultivar 'Lira 45' and the validation of the corresponding deduced amino acid sequences by using MALDI-TOF and LC-MS/MS. Four nucleotide differences were identified in the Bx20 gene with respect to the deduced sequence present in NCBI, causing two amino acid substitutions. For the By20 subunit, nucleotide and amino acid sequences revealed a great similarity to By15, both at gene and protein levels, showing five nucleotide changes generating two amino acid differences. No evidence of post-translational modifications has been found. Hypotheses are formulated in regard to relationships with technological quality. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: DAFNE, Department of Agricultural and Forestry Sciences, Tuscia University, Via San Camillo de Lellis snc, Viterbo, 01100, Italy.
authors.forename: Vito Davide
authors.initials: VD
authors.lastname: Santagati
authors.affiliation: DAFNE, Department of Agricultural and Forestry Sciences, Tuscia University, Via San Camillo de Lellis snc, Viterbo, 01100, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Sestili
authors.affiliation: DAFNE, Department of Agricultural and Forestry Sciences, Tuscia University, Via San Camillo de Lellis snc, Viterbo, 01100, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Lafiandra
authors.affiliation: DAFNE, Department of Agricultural and Forestry Sciences, Tuscia University, Via San Camillo de Lellis snc, Viterbo, 01100, Italy.
authors.forename: Renato
authors.identifier: http://orcid.org/0000-0001-8530-5898
authors.initials: R
authors.lastname: D'Ovidio
authors.affiliation: INRA, UR1268 Biopolymers Interactions Assemblies, Rue de la Géraudière, Nantes Cedex 3, 44316, France.
authors.forename: Helene
authors.initials: H
authors.lastname: Rogniaux
authors.affiliation: DAFNE, Department of Agricultural and Forestry Sciences, Tuscia University, Via San Camillo de Lellis snc, Viterbo, 01100, Italy.
authors.forename: Stefania
authors.identifier: http://orcid.org/0000-0003-2857-4498
authors.initials: S
authors.lastname: Masci
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003545:Cysteine / Q000737:chemistry; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D017343:Genes, Plant; D005983:Glutens / Q000737:chemistry / Q000235:genetics*; D008970:Molecular Weight; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000737:chemistry / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D021122:Protein Subunits; D005983:Glutens; C037302:glutenin; D003545:Cysteine
keywords: cysteine residues; dough quality; glutenin polymers; high molecular weight glutenin subunits; wheat