title: Surface immobilization of kanamycin-chitosan nanoparticles on polyurethane ureteral stents to prevent bacterial adhesion.
abstract: Bacterial adhesion is a major problem that can lead to the infection of implanted urological stents. In this study, kanamycin-chitosan nanoparticles (KMCSNPs) were immobilized on the surface of a polyurethane ureteral stent (PUS) to prevent urinary bacterial infection. KMCSNPs were synthesized using the ionic gelation method. The synthesized KMCSNPs appeared spherical with a ζ-average particle size of 225 nm. KMCSNPs were immobilized on the PUS surface by covalent immobilization techniques. The surface-modified PUS was characterized using attenuated total reflectance Fourier transform infrared spectroscopy, field emission scanning electron microscopy, and energy dispersive X-ray spectroscopy. The surface-modified PUS showed significantly increased antibacterial activity against Escherichia coli MTCC 729 and Proteus mirabilis MTCC 425 relative to the surface of an unmodified PUS. These findings suggest that the KMCSNP-immobilized PUS has the potential to prevent bacterial infection in the human urinary tract.
authors.affiliation: a Department of Biotechnology, School of Bioengineering , SRM University , Kattankulathur , Tamil Nadu , India.
authors.forename: Govindarajan
authors.initials: G
authors.lastname: Venkat Kumar
authors.affiliation: b Department of Chemical Engineering , Ming Chi University of Technology , Taishan , Taipei , Taiwan.
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Su
authors.affiliation: a Department of Biotechnology, School of Bioengineering , SRM University , Kattankulathur , Tamil Nadu , India.
authors.forename: Palaniyandi
authors.initials: P
authors.lastname: Velusamy
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D007612:Kanamycin / Q000737:chemistry / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011140:Polyurethanes / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D015607:Stents / Q000382:microbiology*; D014513:Ureter / Q000382:microbiology*; D014552:Urinary Tract Infections / Q000517:prevention & control
chemical_list: D000900:Anti-Bacterial Agents; D011140:Polyurethanes; D007612:Kanamycin; D048271:Chitosan
keywords: Keywords; Polyurethane ureteral stent; antibacterial assay; in vitro drug release; kanamycin-chitosan nanoparticles; surface modification