title: Preparation of a novel Zr(4+)-immobilized metal affinity membrane for selective adsorption of phosphoprotein.
abstract: In this study, a novel phosphate-Zr(4+) immobilized metal affinity membrane (IMAM) was prepared based on the surface initiated-atom transfer radical polymerization technique for the selective adsorption of phosphoprotein. The adsorption capacity and selectivity of the phosphate-Zr(4+) IMAM were evaluated by using the mixture of standard phosphoproteins (β-casein, ovalbumin) and nonphosphoproteins (bovine serum albumin and lysozyme) as model samples. The adsorption isotherms and competitive adsorption results demonstrated that the phosphate-Zr(4+) IMAM had higher binding capacity and selectivity for phosphoproteins over nonphosphoproteins. Moreover, the phosphate-Zr(4+) IMAM exhibited good re-usability and re-productivity. Finally, the phosphate-Zr(4+) IMAM was applied to separate phosphoprotein from real samples with high purity. Therefore, the as-prepared phosphate-Zr(4+) IMAM could be a promising affinity material for the efficient enrichment of phosphoprotein from complex bio-samples.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, China.
authors.forename: Maofang
authors.initials: M
authors.lastname: He
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, China. Electronic address: czwang@nwu.edu.cn.
authors.forename: Chaozhan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, China. Electronic address: ymwei@nwu.edu.cn.
authors.forename: Yinmao
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000327:Adsorption; D000818:Animals; D002364:Caseins / Q000302:isolation & purification; D002412:Cations / Q000737:chemistry; D005591:Chemical Fractionation; D002645:Chickens; D004527:Egg Proteins / Q000302:isolation & purification; D004529:Egg White / Q000737:chemistry; D004852:Epoxy Compounds / Q000737:chemistry*; D008567:Membranes, Artificial; D008689:Methacrylates / Q000737:chemistry*; D010047:Ovalbumin / Q000302:isolation & purification; D010710:Phosphates / Q000737:chemistry*; D010750:Phosphoproteins / Q000302:isolation & purification*; D058105:Polymerization; D015040:Zirconium / Q000737:chemistry*
chemical_list: D002364:Caseins; D002412:Cations; D004527:Egg Proteins; D004852:Epoxy Compounds; D008567:Membranes, Artificial; D008689:Methacrylates; D010710:Phosphates; D010750:Phosphoproteins; D010047:Ovalbumin; D015040:Zirconium; C007870:glycidyl methacrylate
keywords: Adsorption; Immobilized metal affinity membrane; Phosphoprotein; Surface initiated-atom transfer radical polymerization