title: Membrane-bound NAC transcription factors in maize and their contribution to the oxidative stress response.
abstract: NAC membrane-bound transcription factors (NTM1-like, NTL proteins) participate in the regulation of plant development and the abiotic stress response. While their function has been thoroughly explored in Arabidopsis thaliana, this is not the case in maize. Seven ZmNTL genes were identified by an in silico scan of relevant genome sequence. All seven included a NAC domain at their N terminus, and an α-helical membrane-bound structure domain in their C terminal region. Based on their gene structure and content of conserved motifs, the seven sequences were distributed into four clades. Six of the seven ZmNTLs were associated with the plasma membrane, and the remaining one with the endoplasmic reticulum. ZmNTL2-7 were more strongly transcribed in the stem than in either the leaf or root, while ZmNTL1 transcript abundance was highest in the leaf. When the plants were exposed to either abscisic acid or hydrogen peroxide treatment, all seven genes were up-regulated in the root and stem and down-regulated in the leaf. The heterologous expression of ZmNTL1-ΔTM, 2-ΔTM and 5-ΔTM in A. thaliana reduced the level of sensitivity of the plant to hydrogen peroxide.
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, School of Life Sciences, Shandong University, Shanda South Road 27, Jinan 250100, Shandong, China; The State Key Lab of Crop Biology, College of Life Sciences, Shandong Agricultural University, Daizong Street 61, Taian 271018, Shandong, China; Department of Resources and Environment, Heze University, Daxue Road 2269, Heze 274000, Shandong, China.
authors.forename: Dexin
authors.initials: D
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, School of Life Sciences, Shandong University, Shanda South Road 27, Jinan 250100, Shandong, China.
authors.forename: Yanchong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, School of Life Sciences, Shandong University, Shanda South Road 27, Jinan 250100, Shandong, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, School of Life Sciences, Shandong University, Shanda South Road 27, Jinan 250100, Shandong, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Li
authors.affiliation: The State Key Lab of Crop Biology, College of Life Sciences, Shandong Agricultural University, Daizong Street 61, Taian 271018, Shandong, China. Electronic address: wangzeli@sdau.edu.cn.
authors.forename: Zeli
authors.initials: Z
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, School of Life Sciences, Shandong University, Shanda South Road 27, Jinan 250100, Shandong, China. Electronic address: xfn0990@sdu.edu.cn.
authors.forename: Fengning
authors.initials: F
authors.lastname: Xiang
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013312:Stress, Physiological; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
chemical_list: D026901:Membrane Transport Proteins; D010940:Plant Proteins
keywords: Abiotic stress; Maize; Membrane-bound; NAC transcription factor; Oxidative stress