title: Human Cytomegalovirus DNA Quantification and Gene Expression in Gliomas of Different Grades.
abstract: Gliomas are the most common type of primary brain tumors. The most aggressive type, Glioblastoma multiforme (GBM), is one of the deadliest human diseases, with an average survival at diagnosis of about 1 year. Previous evidence suggests a link between human cytomegalovirus (HCMV) and gliomas. HCMV has been shown to be present in these tumors and several viral proteins can have oncogenic properties in glioma cells. Here we have investigated the presence of HCMV DNA, RNA and proteins in fifty-two gliomas of different grades of malignancy. The UL83 viral region, the early beta 2.7 RNA and viral protein were detected in 73%, 36% and 57% by qPCR, ISH and IHC, respectively. Positivity of the viral targets and viral load was independent of tumor type or grade suggesting no correlation between viral presence and tumor progression. Our results demonstrate high prevalence of the virus in gliomas from Brazilian patients, contributing to a better understanding of the association between HCMV infection and gliomas worldwide and supporting further investigations of the virus oncomodulatory properties.
authors.affiliation: Laboratório de Biologia Molecular de Patógenos, Virologia Molecular, Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, São Paulo, Brazil.
authors.forename: Lucas Matheus
authors.initials: LM
authors.lastname: Stangherlin
authors.affiliation: Laboratório de Biologia Molecular de Patógenos, Virologia Molecular, Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, São Paulo, Brazil.
authors.forename: Fabiane Lucy Ferreira
authors.initials: FL
authors.lastname: Castro
authors.affiliation: Departamento de Anatomia Patológica do Hospital das Clínicas da Faculdade Medicina da Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Raphael Salles Scortegagna
authors.initials: RS
authors.lastname: Medeiros
authors.affiliation: Núcleo de Patologia Quantitativa (NPQ) do Centro de Patologia do Instituto Adolfo Lutz (IAL), São Paulo, Brazil.
authors.forename: Juliana Mariotti
authors.initials: JM
authors.lastname: Guerra
authors.affiliation: Núcleo de Patologia Quantitativa (NPQ) do Centro de Patologia do Instituto Adolfo Lutz (IAL), São Paulo, Brazil.
authors.forename: Lidia Midori
authors.initials: LM
authors.lastname: Kimura
authors.affiliation: Núcleo de Patologia Quantitativa (NPQ) do Centro de Patologia do Instituto Adolfo Lutz (IAL), São Paulo, Brazil.
authors.forename: Neuza Kazumi
authors.initials: NK
authors.lastname: Shirata
authors.affiliation: Núcleo de Patologia Quantitativa (NPQ) do Centro de Patologia do Instituto Adolfo Lutz (IAL), São Paulo, Brazil.
authors.forename: Suely
authors.initials: S
authors.lastname: Nonogaki
authors.affiliation: Laboratório de Biologia Molecular de Patógenos, Virologia Molecular, Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, São Paulo, Brazil.
authors.forename: Claudia Januário
authors.initials: CJ
authors.lastname: Dos Santos
authors.affiliation: Laboratório de Biologia Molecular de Patógenos, Virologia Molecular, Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, São Paulo, Brazil.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Carlan Silva
mesh_terms: D001938:Brazil; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000821:virology*; D004279:DNA, Viral; D015870:Gene Expression; D005910:Glioma / Q000209:etiology* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D060787:Neoplasm Grading; D012367:RNA, Viral; D019562:Viral Load; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins