title: De Novo Characterization of Genes That Contribute to High-Level Ciprofloxacin Resistance in Escherichia coli.
abstract: Sensitization of resistant bacteria to existing antibiotics depends on the identification of candidate targets whose activities contribute to resistance. Using a transposon insertion library in an Escherichia coli mutant that was 2,000 times less susceptible to ciprofloxacin than its parent and the relative fitness scores, we identified 19 genes that contributed to the acquired ciprofloxacin resistance and mapped the shortest genetic path that increased the antibiotic susceptibility of the resistant bacteria back to a near wild-type level.
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics and Biotechnology Institute, University of Minnesota, St. Paul, Minnesota, USA.
authors.forename: Thu
authors.initials: T
authors.lastname: Tran
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics and Biotechnology Institute, University of Minnesota, St. Paul, Minnesota, USA.
authors.forename: Qinghong
authors.initials: Q
authors.lastname: Ran
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics and Biotechnology Institute, University of Minnesota, St. Paul, Minnesota, USA.
authors.forename: Lev
authors.initials: L
authors.lastname: Ostrer
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics and Biotechnology Institute, University of Minnesota, St. Paul, Minnesota, USA khodu001@umn.edu.
authors.forename: Arkady
authors.initials: A
authors.lastname: Khodursky
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology*; D004251:DNA Transposable Elements; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008826:Microbial Sensitivity Tests; D009154:Mutation
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D002939:Ciprofloxacin