title: [Effect of parathyroid hormone (1-34) on expression of matrix Gla protein and Wnt/β catenin signaling pathways in MG63 cell lines].
abstract: OBJECTIVE
  To observe the effect of parathyroid hormone (PTH)(1-34) on the expression of matrix Gla protein (MGP) and Wnt/β-catenin signaling pathway and elucidate the possible molecular mechanism of PTH (1-34) in the prevention and treatment of osteoporosis.
  
  
  METHODS
  MG63 cells treated with PTH (1-34) at 10(-9), 10(-8), and 10(-7) mol/L, alone or in combination with Wnt/β-catenin signaling pathway inhibitors DKK-1 (200 ng/ml) were examined for mRNA and protein expressions related with Wnt/β-catenin signaling with real-time PCR and Western blotting. The cell differentiation after the treatment was assessed with alkaline phosphatase (ALP) staining and cell viability assay.
  
  
  RESULTS
  PTH (1-34) significantly increased the expression of MGP in a dose-dependent manner in MG63 cells (P<0.05 or P<0.01). PTH treatment obviously enhanced ALP activity in the cells, and this effect was suppressed by DKK-1. Combined treatment with DKK-1 partially blocked PTH-induced enhancement of ALP activity (P<0.05). PTH promoted the expression of MGP and enhanced LRP5, β-catenin, and Runx2 expressions in Wnt/β-catenin signaling pathway at both protein and mRNA levels (P<0.05 or P<0.01). DKK-1 partially blocked the effect of PTH (1-34) on Wnt/β-catenin signaling pathway (P<0.05) without affecting MGP expression.
  
  
  CONCLUSIONS
  PTH (1-34) significantly increases the expressions of MGP and proteins in the Wnt/β-catenin signaling pathway. Wnt/β-catenin signaling pathway and MGP mediate the regulation of osteogenosis by PTH.
authors.affiliation: Department of Endocrinology and Metabolism, Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.E-mail: huyali7312@126.com.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Hu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Liu-Chen
authors.initials: LC
authors.lastname: Fu
authors.forename: Ya
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002470:Cell Survival; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D010012:Osteogenesis; D010024:Osteoporosis; D010281:Parathyroid Hormone / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D060449:Wnt Signaling Pathway; D000097568:Matrix Gla Protein
chemical_list: D000469:Alkaline Phosphatase; D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; D036341:Intercellular Signaling Peptides and Proteins; D010281:Parathyroid Hormone; D000097568:Matrix Gla Protein; C501126:DKK1 protein, human