title: Chasing the precursor of functional hematopoietic stem cells at the single cell levels in mouse embryos.
abstract: Adult hematopoietic stem cells (HSCs), the ideal system for regenerative research, were isolated at single cell levels decades ago, whereas studies on embryonic HSCs are much more difficult.
  
  
  
  Zhou et al identified a new pre-HSC cell surface marker, CD201, by which they isolated pre-HSCs at single cell levels for further analyses.
  
  
  
  The novel expression pattern of HSC development is revealed, including the fundamental role of mammalian targets of rapamycin (mTOR) signaling pathway in HSCs emergence, and the repopulation potential of S/G2/M phase pre-HSCs.
  
  
  
  Deeper understandings of the cellular origin and developmental regulatory network of HSCs are essential to develop new strategies of generating HSCs in vitro for clinical application.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300020, China. xcwang@ihcams.ac.cn.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300020, China.
authors.forename: Yuemin
authors.initials: Y
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300020, China.
authors.forename: Hideo
authors.initials: H
authors.lastname: Ema
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology*; D000074283:Endothelial Protein C Receptor / Q000032:analysis*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D000066449:Human Embryonic Stem Cells; D006801:Humans; D051379:Mice; D000066450:Mouse Embryonic Stem Cells; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000502:physiology
chemical_list: D000074283:Endothelial Protein C Receptor; D058570:TOR Serine-Threonine Kinases
keywords: Cell-cycle; HSC; Pre-HSC; mTOR