title: [The effect and injury to the airways of different sputum aspiration pressure in a rat model of mucus hypersecretion].
abstract: OBJECTIVE
  To explore the optimal pressure of sputum aspiration to ensure the effectiveness and safety of clinical operation.
  
  
  METHODS
  We established a rabbit model of airway mucus hypersecretion by aerosol acrolein inhalation, and the animals were divided into 4 groups randomly with different sputum aspiration pressure as follows: group A -75 mmHg (1 mmHg=0.133 kPa), group B -150 mmHg, group C -225 mmHg, group D -300 mmHg. Sputum aspiration efficiency and tracheal mucosal damage degree were evaluated by sputum volume, oxygen saturation changes, the pathological sections of tracheal mucosa and the expressions of IL-1β and TNF-α in airway secretion.
  
  
  RESULTS
  The sputum suction volume of group A, B, C, D were (2.72±0.24), (4.81±0.32), (5.03±0.37) and (6.29±0.51) ml, respectively, which was significantly higher in group D, but lower in group A, as compared to other groups (P<0.05). There were no significant differences between B and C groups. The maximal SpO2 decrease of C and D groups [(18.1±5.2)% and(32.4±8.4)%]were significantly higher than those in A and B groups [(4.4±1.7)% and (6.3±2.9)%], and group D was significantly more than group C, and the difference was statistically significant (P<0.05). There were no significant differences between A and B groups. HE staining of tracheal mucosa in C and D groups showed that the inflammatory cell infiltration and mucosal damage were more serious than A and B groups, but the airway mucosal damage of group A was the least.
  
  
  CONCLUSIONS
  The pressure of -150 mmHg was more effective with high oxygen saturation and less airway injury, which may be suitable for clinical sputum aspiration.
authors.affiliation: Department of Nursing, the Third Affiliated Hospital of Zunyi Medical University, Zunyi 563002, China.
authors.forename: R X
authors.initials: RX
authors.lastname: Wang
authors.forename: J L
authors.initials: JL
authors.lastname: Shen
authors.forename: D S
authors.initials: DS
authors.lastname: Liu
authors.forename: C M
authors.initials: CM
authors.lastname: Li
mesh_terms: D000818:Animals; D001707:Biopsy, Needle / Q000379:methods*; D004195:Disease Models, Animal; D053583:Interleukin-1beta / Q000378:metabolism; D009093:Mucus / Q000378:metabolism*; D011312:Pressure; D011897:Random Allocation; D051381:Rats; D012137:Respiratory System / Q000293:injuries*; D013183:Sputum; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha