title: Microextraction by packed sorbent and HPLC-PDA quantification of multiple anti-inflammatory drugs and fluoroquinolones in human plasma and urine.
abstract: We developed and validated an analytical method based on microextraction packed sorbent (MEPS) and high-performance liquid chromatography (HPLC) coupled to photodiode array (PDA) detector to simultaneously quantify multiple nonsteroidal anti-inflammatory drugs (NSAIDs) and fluoroquinolones (FLQs), which may provide as combination several adverse reactions in nephrology and neurology. The linearity range from LOQs (0.1 μg/mL) to 10 μg/mL, and LODs values were 0.03 μg/mL for both NSAIDs and FLQs. The validation was performed according to international guidelines and the accuracy was tested measuring the precision, intermediate precision and trueness. The drugs stability was tested under different storage conditions (+4 °C and -20 °C) and after three different cycles of freezing and thawing. The method can be a suitable tool to simultaneously detect a possible association of drugs in human biological samples and provide several potentialities for clinical applications, bioequivalence studies, pharmacodynamics and toxicodynamics of different pharmaceutical dosage forms showing NSAIDs and FLQs.
authors.affiliation: a Department of Pharmacy , University "G. d'Annunzio" of Chieti-Pescar , Chieti , Italy.
authors.forename: Veronica
authors.initials: V
authors.lastname: D'Angelo
authors.affiliation: a Department of Pharmacy , University "G. d'Annunzio" of Chieti-Pescar , Chieti , Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Tessari
authors.affiliation: a Department of Pharmacy , University "G. d'Annunzio" of Chieti-Pescar , Chieti , Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Bellagamba
authors.affiliation: a Department of Pharmacy , University "G. d'Annunzio" of Chieti-Pescar , Chieti , Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: De Luca
authors.affiliation: a Department of Pharmacy , University "G. d'Annunzio" of Chieti-Pescar , Chieti , Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Cifelli
authors.affiliation: a Department of Pharmacy , University "G. d'Annunzio" of Chieti-Pescar , Chieti , Italy.
authors.forename: Christian
authors.initials: C
authors.lastname: Celia
authors.affiliation: a Department of Pharmacy , University "G. d'Annunzio" of Chieti-Pescar , Chieti , Italy.
authors.forename: Rosita
authors.initials: R
authors.lastname: Primavera
authors.affiliation: a Department of Pharmacy , University "G. d'Annunzio" of Chieti-Pescar , Chieti , Italy.|b Department of Health Sciences , and.
authors.forename: Martina
authors.initials: M
authors.lastname: Di Francesco
authors.affiliation: c Department of Clinical and Experimental Medicine , University of Catanzaro "Magna Graecia" , Catanzaro , Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: Paolino
authors.affiliation: a Department of Pharmacy , University "G. d'Annunzio" of Chieti-Pescar , Chieti , Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Di Marzio
authors.affiliation: a Department of Pharmacy , University "G. d'Annunzio" of Chieti-Pescar , Chieti , Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Locatelli
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000097:blood* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000097:blood* / Q000652:urine*; D064368:Healthy Volunteers; D006801:Humans; D059627:Liquid Phase Microextraction; D015394:Molecular Structure
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D024841:Fluoroquinolones
keywords: MEPS–HPLC–PDA; NSAIDs and fluoroquinolones; method validation; plasma and urine; sample preparation