title: [Docosahexaenoic acid inhibits aflatoxin B1-induced migration and invasion in hepatocellular carcinoma cells in vitro].
abstract: OBJECTIVE
  To investigate the effect of docosahexaenoic acid (DHA) on invasiveness of aflatoxin B1 (AFB1)-induced hepatocellular carcinoma cells in vitro.
  
  
  METHODS
  HepG2.2.15 cells were exposed to different concentrations of AFB1 and DHA plus AFB1. The cell migration and invasion were assessed using wound-healing and Transwell assay, and flow cytometry was used to analyze the cell cycle changes. The ultrastructural changes of the cells were observed by transmission electron microscopy.
  
  
  RESULTS
  Compared with the control group, the cells exposed to2 µmol/L AFB1 showed obviously enhanced migration and invasion with decreased cell ratio in G1/G1 phase and increased cell ratio in G2/M phase but no changes in S phase cells; transmission electron microscopy revealed the presence of multiple nucleoli and significantly increased mitochondria and Golgi apparatus in the exposed cells. Compared with AFB1-exposed cells, the cells treated with DHA and AFB1 showed decreased migration and invasion abilities, and the G1/G1 phase cells increased and G2/M phase cells decreased significantly; ultrastructurally, the cells contained single nucleoli with decreased mitochondria and vacuolization occurred in the cytoplasm.
  
  
  CONCLUSIONS
  DHA can significantly inhibit AFB1-induced enhancement of cell migration and invasion in hepatocellular carcinoma cells in vitro.
authors.affiliation: Department of Chemotherapy, Guangxi Zhuang Autonomous Region People's Hospital, Nanning 530021, China.E-mail: ckhxy@163.com.
authors.forename: Ke-He
authors.initials: KH
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Gao
authors.forename: Jin-Fei
authors.initials: JF
authors.lastname: Pan
authors.forename: Hai-Ming
authors.initials: HM
authors.lastname: Wei
authors.forename: Chun-Hong
authors.initials: CH
authors.lastname: Jia
authors.forename: Jiao
authors.initials: J
authors.lastname: Lan
authors.forename: Zhen-Xiang
authors.initials: ZX
authors.lastname: Chen
authors.forename: Deng
authors.initials: D
authors.lastname: Pan
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Bai
mesh_terms: D016604:Aflatoxin B1 / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002453:Cell Cycle; D002465:Cell Movement / Q000187:drug effects*; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D006056:Golgi Apparatus; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008928:Mitochondria; D009361:Neoplasm Invasiveness
chemical_list: D004281:Docosahexaenoic Acids; D016604:Aflatoxin B1