title: Analysis of the Expressions of TrkB and GLT-1 in Brain Tissues on a Rat Pentylenetetrazol Kindling Model of Chronic Epilepsy.
abstract: OBJECTIVE
  Tyrosine kinase receptor-B (TrkB), a high-affinity receptor for brain-derived neurotrophic factor (BDNF), functions via specific binding with the BDNF. Although studies have shown that BDNF promotes the expression of glutamate transporter-1 (GLT-1) in the glial cells during neurodegeneration, evidence is lacking regarding whether BDNF/TrkB pathway is associated with the abnormal function of GLT-1.The aim of this study is to analyze the expressions of TrkB and GLT-1 in the brain of pentylenetetrazol (PTZ) kindling model of chronic epilepsy in rats, and to provide evidence for the correlation between the BDNF/TrkB pathway and GLT-1 and clues for the prevention and treatment targets of epilepsy.
  
  
  METHODS
  In total, 40 Sprague-Dawley rats were randomly classified as model (n= 30) and control (n= 10) groups respectively. Western blotting was used for analyzing the expression of TrkB and GLT-1, and double immunofluorescence staining was used for detecting the cells positive for both glial fibrillary acidic protein (GFAP) and TrkB in the hippocampus and temporal cortex 7 days after establishing the kindling model.
  
  
  RESULTS
  Compared with the control group, protein level expression of TrkB and GLT-1, and mean optical density (OD) value of the cells positive for both GFAP and TrkB in the hippocampus and temporal cortex were significantly increased after the onset of epilepsy (P < 0.05).
  
  
  CONCLUSIONS
  The BDNF/TrkB pathway may participate in the epileptogenesis through modulating the biological effect of GLT-1 in the glial cells of PTZ kindling rats, though the specific mechanisms require further investigations.
authors.affiliation: Sichuan Provincial People's Hospital, Department of Neurosurgery, First Ring Road, Chengdu, Sichuan, 610072, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
authors.forename: Chao
authors.initials: C
authors.lastname: You
authors.forename: Zhong
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D003292:Convulsants / Q000494:pharmacology; D004195:Disease Models, Animal; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism*; D027342:Excitatory Amino Acid Transporter 2 / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D007696:Kindling, Neurologic / Q000187:drug effects; D008297:Male; D010433:Pentylenetetrazole / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020813:Receptor, trkB / Q000378:metabolism*; D013702:Temporal Lobe / Q000378:metabolism*
chemical_list: D003292:Convulsants; D027342:Excitatory Amino Acid Transporter 2; D020813:Receptor, trkB; D010433:Pentylenetetrazole