title: Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54.
abstract: Filamentous plant pathogens deliver effector proteins to host cells to promote infection. The Phytophthora infestans RXLR-type effector PexRD54 binds potato ATG8 via its ATG8 family-interacting motif (AIM) and perturbs host-selective autophagy. However, the structural basis of this interaction remains unknown. Here, we define the crystal structure of PexRD54, which includes a modular architecture, including five tandem repeat domains, with the AIM sequence presented at the disordered C terminus. To determine the interface between PexRD54 and ATG8, we solved the crystal structure of potato ATG8CL in complex with a peptide comprising the effector's AIM sequence, and we established a model of the full-length PexRD54-ATG8CL complex using small angle x-ray scattering. Structure-informed deletion of the PexRD54 tandem domains reveals retention of ATG8CL binding in vitro and in planta This study offers new insights into structure/function relationships of oomycete RXLR effectors and how these proteins engage with host cell targets to promote disease.
authors.affiliation: From the Department of Biological Chemistry, John Innes Centre, and.
authors.forename: Abbas
authors.identifier: 0000-0002-6189-5560
authors.initials: A
authors.lastname: Maqbool
authors.affiliation: From the Department of Biological Chemistry, John Innes Centre, and.
authors.forename: Richard K
authors.identifier: 0000-0001-9910-6566
authors.initials: RK
authors.lastname: Hughes
authors.affiliation: the Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom.
authors.forename: Yasin F
authors.identifier: 0000-0002-9502-355X
authors.initials: YF
authors.lastname: Dagdas
authors.affiliation: From the Department of Biological Chemistry, John Innes Centre, and.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Tregidgo
authors.affiliation: the Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom.
authors.forename: Erin
authors.initials: E
authors.lastname: Zess
authors.affiliation: the Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom.
authors.forename: Khaoula
authors.initials: K
authors.lastname: Belhaj
authors.affiliation: EMBL Grenoble, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France, and.|EPSAM, Keele University, Keele ST5 5GB, United Kingdom.
authors.forename: Adam
authors.identifier: 0000-0002-0723-8228
authors.initials: A
authors.lastname: Round
authors.affiliation: the Department of Life Sciences, Imperial College, London SW7 2AZ, United Kingdom.
authors.forename: Tolga O
authors.identifier: 0000-0003-0507-6875
authors.initials: TO
authors.lastname: Bozkurt
authors.affiliation: the Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom.
authors.forename: Sophien
authors.identifier: 0000-0002-0290-0315
authors.initials: S
authors.lastname: Kamoun
authors.affiliation: From the Department of Biological Chemistry, John Innes Centre, and mark.banfield@jic.ac.uk.
authors.forename: Mark J
authors.identifier: 0000-0001-8921-3835
authors.initials: MJ
authors.lastname: Banfield
mesh_terms: D000071190:Autophagy-Related Protein 8 Family / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D055750:Phytophthora infestans / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000072417:Protein Domains; D020836:Protein Structure, Quaternary; D011198:Solanum tuberosum / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D000071190:Autophagy-Related Protein 8 Family; D010940:Plant Proteins
keywords: autophagy; effector protein; host-pathogen interaction; plant molecular biology; plant pathogen; protein structure; protein-protein interaction