title: Short Chain Fatty Acids (SCFA) Reprogram Gene Expression in Human Malignant Epithelial and Lymphoid Cells.
abstract: The effect of short chain fatty acids (SCFAs) on gene expression in human, malignant cell lines was investigated, with a focus on signaling pathways. The commensal microbial flora produce high levels of SCFAs with established physiologic effects in humans. The most abundant SCFA metabolite in the human microflora is n-butyric acid. It is well known to activate endogenous latent Epstein-Barr virus (EBV), that was used as a reference read out system and extended to EBV+ epithelial cancer cell lines. N-butyric acid and its salt induced inflammatory and apoptotic responses in tumor cells of epithelial and lymphoid origin. Epithelial cell migration was inhibited. The n-butyric gene activation was reduced by knock-down of the cell membrane transporters MCT-1 and -4 by siRNA. N-butyric acid show biologically significant effects on several important cellular functions, also with relevance for tumor cell phenotype.
authors.affiliation: Institute of Food Science and Technology, Kemerovo, Russia.|Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
authors.forename: Lidiia
authors.initials: L
authors.lastname: Astakhova
authors.affiliation: Department of Cell and Molecular Biology (CMB), Ludwig Institute for Cancer Research (LICR), Karolinska Institutet, Stockholm, Sweden.
authors.forename: Mtakai
authors.initials: M
authors.lastname: Ngara
authors.affiliation: Institute of Food Science and Technology, Kemerovo, Russia.
authors.forename: Olga
authors.initials: O
authors.lastname: Babich
authors.affiliation: Institute of Food Science and Technology, Kemerovo, Russia.
authors.forename: Aleksandr
authors.initials: A
authors.lastname: Prosekov
authors.affiliation: Institute of Food Science and Technology, Kemerovo, Russia.
authors.forename: Lyudmila
authors.initials: L
authors.lastname: Asyakina
authors.affiliation: Institute of Food Science and Technology, Kemerovo, Russia.
authors.forename: Lyubov
authors.initials: L
authors.lastname: Dyshlyuk
authors.affiliation: Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
authors.forename: Tore
authors.initials: T
authors.lastname: Midtvedt
authors.affiliation: Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
authors.forename: Ingemar
authors.initials: I
authors.lastname: Ernberg
authors.affiliation: Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
authors.forename: Liudmila
authors.initials: L
authors.lastname: Matskova
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D020148:Butyric Acid / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000473:pathology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D005232:Fatty Acids, Volatile / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007249:Inflammation / Q000473:pathology; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000473:pathology; D026901:Membrane Transport Proteins / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D005232:Fatty Acids, Volatile; D026901:Membrane Transport Proteins; D016328:NF-kappa B; D020148:Butyric Acid; D011065:Poly(ADP-ribose) Polymerases