title: In vitro and in vivo anti-Vibrio vulnificus activity of psammaplin A, a natural marine compound.
abstract: Vibrio vulnificus is known to induce severely fulminant and fatal septicemia in susceptible hosts. In the present study, the antimicrobial activity of natural marine product-derived compounds against V. vulnificus, were investigated in vitro and in vivo. Twelve pure compounds were isolated from natural marine products and their inhibitory effects on V. vulnificus-induced cytotoxicity were determined in INT‑407 cells. Among the 12 pure compounds tested, treatment with psammaplin A significantly suppressed V. vulnificus‑induced cytotoxicity in INT‑407 cells. Notably, treatment with psammaplin A (5-50 µg) had improved survival rates compared with that in the untreated mice, when the mice were infected with V. vulnificus intraperitoneally. In addition, the bacterial load of V. vulnificus in several tissues (spleen, liver and small intestine) was significantly lower in psammaplin A‑treated mice than in untreated mice. Furthermore, psammaplin A treatment significantly suppressed the growth of V. vulnificus. Taken together, these results indicate that psammaplin A may be a potential agent for the prevention and treatment of V. vulnificus infections.
authors.affiliation: Department of Life Sciences, College of Life Sciences and Biotechnology, Korea University, Seoul 136‑701, Republic of Korea.
authors.forename: Byung Cheol
authors.initials: BC
authors.lastname: Lee
authors.affiliation: Department of Life Sciences, College of Life Sciences and Biotechnology, Korea University, Seoul 136‑701, Republic of Korea.
authors.forename: Arim
authors.initials: A
authors.lastname: Lee
authors.affiliation: Department of Pharmacy, Pusan National University, Geumjeong‑gu, Busan 609‑735, Republic of Korea.
authors.forename: Jee Hyung
authors.initials: JH
authors.lastname: Jung
authors.affiliation: National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology, Seoul National University, Seoul 151‑921, Republic of Korea.
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Department of Life Sciences, College of Life Sciences and Biotechnology, Korea University, Seoul 136‑701, Republic of Korea.
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Kim
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D059001:Aquatic Organisms / Q000737:chemistry*; D001688:Biological Products / Q000494:pharmacology*; D002460:Cell Line; D004195:Disease Models, Animal; D004220:Disulfides / Q000494:pharmacology*; D004847:Epithelial Cells; D005260:Female; D006801:Humans; D051379:Mice; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014735:Vibrio Infections / Q000188:drug therapy / Q000382:microbiology / Q000401:mortality / Q000473:pathology; D041261:Vibrio vulnificus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D001688:Biological Products; D004220:Disulfides; C098378:psammaplin A; D014443:Tyrosine