title: Complete genome sequence of Bacillus oceanisediminis 2691, a reservoir of heavy-metal resistance genes.
abstract: Ocean sediments are commonly subject to the pollution of various heavy metals. Intracellular heavy metal concentrations in marine microorganisms should be kept within allowable concentrations. Here, we report redundant heavy metal resistance related genes encoding heavy metal-sensing transcriptional regulators (i.e. cadC), heavy metal efflux pumps, and detoxifying enzymes in the complete genome sequence of Bacillus oceanisediminis 2691. By comparing CadC sequences of strain 2691 with those from other bacterial genomes, we demonstrated that each cadC gene located in the chromosome or plasmid of 2691 cells are similar to those of various near or distant microbes, which might shed light on evolutionary trajectories of redundant heavy metal resistance genes. In application aspects, these diverse heavy metal sensing genes can be harnessed as synthetic biological parts, modules, and devices for the development of heavy metal-specific biosensors. Heavy metal bioremediation technologies or platform cells can be also developed based on the marine genomic information of heavy metal resistance and/or detoxification genes in a bacterial isolate from ocean sediments.
authors.affiliation: Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
authors.forename: Jaejoon
authors.initials: J
authors.lastname: Jung
authors.affiliation: Super-Bacteria Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea; Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, Republic of Korea. Electronic address: hyjeong@kribb.re.kr.
authors.forename: Haeyoung
authors.initials: H
authors.lastname: Jeong
authors.affiliation: Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea; Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, Republic of Korea.
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Lee
authors.affiliation: Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea; Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, Republic of Korea. Electronic address: leesj@kribb.re.kr.
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D001407:Bacillus / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D019216:Metals, Heavy; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; C080050:CadC protein, Bacteria; D019216:Metals, Heavy
keywords: Bacillus oceanisediminis; Heavy metal; Sediment; Transcriptional regulator; cadC