title: Peripheral-Nerve and Spinal-Cord Regeneration in Mice Using Hair-Follicle-Associated Pluripotent (HAP) Stem Cells.
abstract: Nestin, a neural stem cell marker protein, is expressed in hair follicle cells above the bulge area. These nestin-positive hair follicle-associated-pluripotent (HAP) stem cells are negative for the keratinocyte marker K15 and can differentiate into neurons, glia, keratinocytes, smooth muscle cells, cardiac muscle cells, and melanocytes in vitro. HAP stem cells are positive for the stem cell marker CD34, as well as K15-negative, suggesting their relatively undifferentiated state. HAP stem cells promoted the functional recovery of injured peripheral nerves and the spinal cord. HAP stem cells differentiated into glial fibrillary acidic protein (GFAP)-positive Schwann cells when implanted in severed sciatic nerves and spinal cords in mice. These results suggest that HAP stem cells provide an important accessible, autologous source of adult stem cells for regenerative medicine, that have critical advantages over ES and iPS stem cells.
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Minami Ward, Sagamihara, 252-0374, Japan.|AntiCancer, Inc., 7917 Ostrow Street, San Diego, CA, 92111, USA.|Department of Surgery, University of California, San Diego, San Diego, CA, 92103, USA.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Amoh
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Minami Ward, Sagamihara, 252-0374, Japan.
authors.forename: Kensei
authors.initials: K
authors.lastname: Katsuoka
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, CA, 92111, USA. all@anticancer.com.|Department of Surgery, University of California, San Diego, San Diego, CA, 92103, USA. all@anticancer.com.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology*; D051379:Mice; D008822:Mice, Transgenic; D009416:Nerve Regeneration; D064231:Nestin / Q000235:genetics; D010525:Peripheral Nerves; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D058630:Spinal Cord Regeneration
chemical_list: D064231:Nestin; D049452:Green Fluorescent Proteins
keywords: Bulge area; Differentiation; Hair follicle; Nerve; Nestin; Pluripotent; Repair; Spinal cord; Stem cell