title: Effect of carbaryl (carbamate insecticide) on acetylcholinesterase activity of two strains of Daphnia magna (Crustacea, Cladocera).
abstract: The present study was designed to investigate the effect of carbaryl (carbamate insecticide) on the acetylcholinesterase activity in two strains (same clone A) of the crustacean cladoceran Daphnia magna. Four carbaryl concentrations (0.4, 0.9, 1.8 and 3.7 µg L(-1)) were compared against control AChE activity. Our results showed that after 48 h of carbaryl exposure, all treatments induced a significant decrease of AChE activities whatever the two considered strains. However, different responses were registered in terms of lowest observed effect concentrations (LOEC: 0.4 µg L(-1) for strain 1 and 0.9 µg L(-1) for strains 2) revealing differences in sensitivity among the two tested strains of D. magna. These results suggest that after carbaryl exposure, the AChE activity responses can be also used as a biomarker of susceptibility. Moreover, our results show that strain1 is less sensitive than strain 2 in terms of IC50-48 h of AChE activity. Comparing the EC50-48 h of standard ecotoxicity test and IC50-48 h of AChE inhibition, there is the same order of sensitivity with both strains.
authors.affiliation: a Laboratoire de Bio-surveillance de l'Environnement (LBE), Université de Carthage, Faculté des Sciences de Bizerte , Zarzouna , Bizerte , Tunisie.|b Laboratoire Interdisciplinaire des Environnements Continentaux, Université de Lorraine , Metz , France.
authors.forename: Hela
authors.initials: H
authors.lastname: Toumi
authors.affiliation: a Laboratoire de Bio-surveillance de l'Environnement (LBE), Université de Carthage, Faculté des Sciences de Bizerte , Zarzouna , Bizerte , Tunisie.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Bejaoui
authors.affiliation: a Laboratoire de Bio-surveillance de l'Environnement (LBE), Université de Carthage, Faculté des Sciences de Bizerte , Zarzouna , Bizerte , Tunisie.
authors.forename: Samir
authors.initials: S
authors.lastname: Touaylia
authors.affiliation: b Laboratoire Interdisciplinaire des Environnements Continentaux, Université de Lorraine , Metz , France.
authors.forename: Karen F
authors.initials: KF
authors.lastname: Burga Perez
authors.affiliation: b Laboratoire Interdisciplinaire des Environnements Continentaux, Université de Lorraine , Metz , France.
authors.forename: Jean François
authors.initials: JF
authors.lastname: Ferard
mesh_terms: D000110:Acetylcholinesterase / Q000187:drug effects* / Q000235:genetics*; D000818:Animals; D012721:Carbaryl / Q000633:toxicity*; D003621:Daphnia / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D014644:Genetic Variation; D007306:Insecticides / Q000633:toxicity*
chemical_list: D007306:Insecticides; D000110:Acetylcholinesterase; D012721:Carbaryl
keywords: Daphnia magna; acetylcholinesterase; carbaryl; intra-strain sensitivity