title: Protein microarray applications: Autoantibody detection and posttranslational modification.
abstract: The discovery of DNA microarrays was a major milestone in genomics; however, it could not adequately predict the structure or dynamics of underlying protein entities, which are the ultimate effector molecules in a cell. Protein microarrays allow simultaneous study of thousands of proteins/peptides, and various advancements in array technologies have made this platform suitable for several diagnostic and functional studies. Antibody arrays enable researchers to quantify the abundance of target proteins in biological fluids and assess PTMs by using the antibodies. Protein microarrays have been used to assess protein-protein interactions, protein-ligand interactions, and autoantibody profiling in various disease conditions. Here, we summarize different microarray platforms with focus on its biological and clinical applications in autoantibody profiling and PTM studies. We also enumerate the potential of tissue microarrays to validate findings from protein arrays as well as other approaches, highlighting their significance in proteomics.
authors.affiliation: Proteomics Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.
authors.forename: Apurva
authors.initials: A
authors.lastname: Atak
authors.affiliation: Proteomics Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.
authors.forename: Shuvolina
authors.initials: S
authors.lastname: Mukherjee
authors.affiliation: Proteomics Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.
authors.forename: Rekha
authors.initials: R
authors.lastname: Jain
authors.affiliation: Proteomics Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.
authors.forename: Shabarni
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Proteomics Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.
authors.forename: Vedita Anand
authors.initials: VA
authors.lastname: Singh
authors.affiliation: Proteomics Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.
authors.forename: Nikita
authors.initials: N
authors.lastname: Gahoi
authors.affiliation: Proteomics Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.
authors.forename: Manubhai
authors.initials: M
authors.lastname: K P
authors.affiliation: Proteomics Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India. sanjeeva@iitb.ac.in.
authors.forename: Sanjeeva
authors.initials: S
authors.lastname: Srivastava
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D011499:Protein Processing, Post-Translational; D046888:Tissue Array Analysis
chemical_list: D001323:Autoantibodies
keywords: Autoantibody profiling; PTM studies; Protein arrays; Tissue microarrays