title: Effects of Alginate Oligosaccharide Mixture on the Bioavailability of Lysozyme as an Antimicrobial Agent.
abstract: In this study, we report an oral drug delivery system without any additional process using pH-sensitive biopolymer, alginate, and alginate oligosaccharide with lysozyme as a model drug. These biopolymers exhibited pH-sensitive characteristics such as shrinking at acidic pH and eroding with dissolution at alkaline pH. The incorporation of lysozyme and biopolymers was performed an artificial intestinal juice (pH 6.8). The immobilization efficiency and lysozyme stability in gastric juice (pH 1.2) has been tested by E coil antimicrobial activity. The lysozyme without biopolymer immobilization lost approximately 80-90% of antimicrobial activity than that of pure lysozyme. However, the pH-sensitive biopolymer-controlled lysozyme maintained similar antimicrobial activity compared to that of pure lysozyme (50-90% of cell mortality). Therefore, this simple, easy, and rapid system can be effectively and practically applied for pathogen treatment for in vivo oral drug delivery.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Joo-Myung
authors.initials: JM
authors.lastname: Ahn
authors.forename: Ra-Mi
authors.initials: RM
authors.lastname: Park
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Simranjeet Singh
authors.initials: SS
authors.lastname: Sekhon
authors.forename: Sang Yong
authors.initials: SY
authors.lastname: Kim
authors.forename: Ji-Hyang
authors.initials: JH
authors.lastname: Wee
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems; D048168:Escherichia coli K12 / Q000254:growth & development*; D005750:Gastric Juice / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000737:chemistry / Q000494:pharmacology; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000464:Alginates; D000890:Anti-Infective Agents; D006603:Hexuronic Acids; D009844:Oligosaccharides; D020723:Glucuronic Acid; D009113:Muramidase