title: Borrelia burgdorferi sensu lato prevalence and diversity in ticks and small mammals in a Lyme borreliosis endemic Nature Reserve in North-Western Spain. Incidence in surrounding human populations.
abstract: To determine the prevalence and diversity of Borrelia burgdorferi sensu lato (s.l.) in an endemic Nature Reserve (Sierra del Sueve) in North-Western Spain, and the risk of human exposure to infected ticks in Asturias, 1013 questing ticks and 70 small mammals were collected between 2012 and 2014. A retrospective descriptive analysis was also carried out on human Lyme borreliosis (LB) cases reported to the local hospital (Cabueñes). Samples were screened for B. burgdorferi s.l. presence by a nested PCR assay, and genospecies were confirmed by sequencing. B. burgdorferi s.l. was detected in 1.4% (12/845) of I. ricinus questing nymphs, 9.1% (2/33) of questing adults, and 12.9% (9/70) of small mammals, as well as in the other tick species. PCR positive samples of 17 questing tick and 6 small mammals were sequenced. Four genospecies were identified: B. afzelii, B. garinii, B. lusitaniae, and B. valaisiana. Phylogenetic analyses based on the flaB gene showed the heterogeneity of B. afzelii in this area. The detection of B. burgdorferi s.l. among questing ticks and small mammals in the study area, as well as the abundance of ticks and of large wild and domestic mammals, indicate a high risk of infection by B. burgdorferi s.l. in the area. Reporting of LB cases to the local hospital support this, and shows the need of thorough monitoring of B. burgdorferi infection in ticks and hosts in the area. More investigations are needed to assess the role of different wildlife species and the risk of transmission to humans.
authors.affiliation: Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Área de Sanidad Animal, Centro de Biotecnología Animal, Deva, Gijón, Asturias, Spain. Electronic address: aespi@serida.org.
authors.forename: Alberto
authors.initials: A
authors.lastname: Espí
authors.affiliation: Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Área de Sanidad Animal, Centro de Biotecnología Animal, Deva, Gijón, Asturias, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Del Cerro
authors.affiliation: Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Área de Cultivos Hortofrutícolas y Forestales, Villaviciosa, Asturias, Spain.
authors.forename: Aitor
authors.initials: A
authors.lastname: Somoano
authors.affiliation: Servicio de Salud del Principado de Asturias, Hospital de Cabueñes, Gijón, Asturias, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: García
authors.affiliation: Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Área de Sanidad Animal, Centro de Biotecnología Animal, Deva, Gijón, Asturias, Spain.
authors.forename: José
authors.initials: J
authors.lastname: M Prieto
authors.affiliation: NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Derio, Bizkaia, Spain.
authors.forename: José F
authors.initials: JF
authors.lastname: Barandika
authors.affiliation: NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Derio, Bizkaia, Spain.
authors.forename: Ana L
authors.initials: AL
authors.lastname: García-Pérez
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D025065:Borrelia burgdorferi / Q000145:classification* / Q000302:isolation & purification*; D002648:Child; D019353:Endemic Diseases; D005260:Female; D006801:Humans; D008193:Lyme Disease / Q000453:epidemiology* / Q000662:veterinary*; D008297:Male; D008322:Mammals / Q000382:microbiology*; D015995:Prevalence; D012189:Retrospective Studies; D013030:Spain / Q000453:epidemiology; D013987:Ticks / Q000382:microbiology*; D055815:Young Adult
keywords: Borrelia burgdorferi sensu lato; Borreliosis de Lyme; Garrapatas; Lyme borreliosis; PCR; Pequeños mamíferos; Small mammals; Ticks