title: 2D FT-ICR MS of Calmodulin: A Top-Down and Bottom-Up Approach.
abstract: Two-dimensional Fourier transform ion cyclotron resonance mass spectrometry (2D FT-ICR MS) allows data-independent fragmentation of all ions in a sample and correlation of fragment ions to their precursors through the modulation of precursor ion cyclotron radii prior to fragmentation. Previous results show that implementation of 2D FT-ICR MS with infrared multi-photon dissociation (IRMPD) and electron capture dissociation (ECD) has turned this method into a useful analytical tool. In this work, IRMPD tandem mass spectrometry of calmodulin (CaM) has been performed both in one-dimensional and two-dimensional FT-ICR MS using a top-down and bottom-up approach. 2D IRMPD FT-ICR MS is used to achieve extensive inter-residue bond cleavage and assignment for CaM, using its unique features for fragment identification in a less time- and sample-consuming experiment than doing the same thing using sequential MS/MS experiments. Graphical Abstract ᅟ.
authors.affiliation: University of Warwick, Coventry, UK.
authors.forename: Federico
authors.initials: F
authors.lastname: Floris
authors.affiliation: University of Warwick, Coventry, UK.
authors.forename: Maria
authors.initials: M
authors.lastname: van Agthoven
authors.affiliation: CASC4DE, Illkirch-Graffenstaden, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Chiron
authors.affiliation: University of Warwick, Coventry, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Soulby
authors.affiliation: University of Warwick, Coventry, UK.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Wootton
authors.affiliation: University of Warwick, Coventry, UK.
authors.forename: Yuko P Y
authors.initials: YP
authors.lastname: Lam
authors.affiliation: University of Warwick, Coventry, UK.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Barrow
authors.affiliation: CASC4DE, Illkirch-Graffenstaden, France.|IGBMC, Illkirch-Graffenstaden, France.
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Delsuc
authors.affiliation: University of Warwick, Coventry, UK. p.oconnor@warwick.ac.uk.
authors.forename: Peter B
authors.initials: PB
authors.lastname: O'Connor
keywords: 2-Dimensional mass spectrometry; FTICR mass spectrometry; Tandem mass spectrometry