title: On-Flow Synthesis of Co-Polymerizable Oligo-Microspheres and Application in ssDNA Amplification.
abstract: We fabricated droplet-based microfluidic platform for copolymerizable microspheres with acrydite modified DNA probe. The copolymerizable 3-D polyacrylamide microspheres were successfully produced from microcontinuous-flow synthesis with on-channel solidification. DNA copolymerization activity, surface presentation and thermostability were assessed by using fluorescent labeled complementary probe. The binding performance was only visible on the surface area of oligo-microspheres. We show that the resulting oligo-microspheres can be directly integrated into a streamlined microsphere-PCR protocol for amplifying ssDNA. Our microspheres could be utilized as a potential material for ssDNA analysis such as DNA microarray and automatic DNA SELEX process.
authors.affiliation: Department of Microbiology, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju 28644, South Korea.
authors.forename: Se Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Graduate School of Mechanical Engineering, Inje University, 197 Inje-ro, Gimhae, Gyungnam 621-749, South Korea.
authors.forename: Jae Ha
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, Inje University, 197 Inje-ro, Gimhae, Gyungnam 621-749, South Korea.
authors.forename: Ho Won
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju 28644, South Korea.
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Graduate School of Mechanical Engineering, Inje University, 197 Inje-ro, Gimhae, Gyungnam 621-749, South Korea.|Department of Biomedical Engineering, Inje University, 197 Inje-ro, Gimhae, Gyungnam 621-749, South Korea.
authors.forename: Ok Chan
authors.initials: OC
authors.lastname: Jeong
authors.affiliation: Department of Microbiology, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju 28644, South Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Ahn
mesh_terms: D015342:DNA Probes; D004261:DNA Replication; D004277:DNA, Single-Stranded; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D008863:Microspheres; D015345:Oligonucleotide Probes; D058105:Polymerization
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded; D015345:Oligonucleotide Probes