title: Decreased expression of the long noncoding RNA LINC00261 indicate poor prognosis in gastric cancer and suppress gastric cancer metastasis by affecting the epithelial-mesenchymal transition.
abstract: Recent evidence indicates that long noncoding RNAs (lncRNAs) play pivotal roles in the regulation of cellular processes and are found to be dysregulated in a variety of cancers. LINC00261 is an lncRNA that is aberrantly expressed in gastric cancer (GC). The clinical role of LINC00261 in GC and molecular mechanisms remains to be found.
  
  
  
  Real-time polymerase chain reaction (PCR) was used to examine LINC00261 expression in GC cell lines/tissues compared with normal epithelial cells/adjacent non-tumorous tissues. Gain and loss of function approaches were used to investigate the biological role of LINC00261 in GC cells. The effects of LINC00261 on cell viability were evaluated by MTT and colony formation assays. Wound healing assay, cell migration and invasion assays, and nude mice were used to examine the effects of LINC00261 on tumor cell metastasis in vitro and in vivo. Protein levels of LINC00261 targets were determined by western blot and immunohistochemistry.
  
  
  
  LINC00261 was downregulated in GC cell lines and cancerous tissues, as compared with normal gastric epithelial cells and adjacent noncancerous tissue samples. Low LINC00261 expression was correlated with deeper tumor invasion (P < 0.001), higher tumor stage (P = 0.013), and lymphatic metastasis (P = 0.006). Univariate and multivariate analyses indicated that low LINC00261 expression predicted poor prognosis. Ectopic expression of LINC00261 impaired cell migration and invasion, leading to the inhibition of metastasis in vitro and in vivo. Knockdown of LINC00261 expression promoted cell migration and invasion in vitro. Overexpression of LINC00261 was found to play a key role in epithelial-mesenchymal transition (EMT) through the regulation of E-cadherin, N-cadherin, and Vimentin expression.
  
  
  
  Low expression of the lncRNA LINC00261 occurs in GC and is associated with poor prognosis. LINC00261 suppresses GC metastasis by regulating EMT. Thus, LINC00261 plays an important role in the progression and metastasis of GC.
authors.affiliation: Cancer Institute, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212002, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Pathology, the First People's Hospital of Yangzhou/The Second Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu, 225000, People's Republic of China.
authors.forename: Yan-Fen
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Radiotherapy and Chemotherapy Department, The 1st Affiliated Hospital of Wenzhou Medical University, No.2 Fuxue Lane, 325000, Wenzhou, China.
authors.forename: Hua-Fang
authors.initials: HF
authors.lastname: Su
authors.affiliation: Cancer Institute, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212002, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Fang
authors.affiliation: Cancer Institute, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212002, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zou
authors.affiliation: Radiotherapy and Chemotherapy Department, The 1st Affiliated Hospital of Wenzhou Medical University, No.2 Fuxue Lane, 325000, Wenzhou, China.
authors.forename: Wen-Feng
authors.initials: WF
authors.lastname: Li
authors.affiliation: Radiotherapy and Chemotherapy Department, The 1st Affiliated Hospital of Wenzhou Medical University, No.2 Fuxue Lane, 325000, Wenzhou, China. hui-y@163.com.
authors.forename: Zheng-Hua
authors.initials: ZH
authors.lastname: Fei
mesh_terms: D000818:Animals; D002465:Cell Movement; D002470:Cell Survival; D002478:Cells, Cultured; D018450:Disease Progression; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D064593:Heterografts; D051379:Mice; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000235:genetics*; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D046888:Tissue Array Analysis
chemical_list: D062085:RNA, Long Noncoding; C000623252:long non-coding RNA 00261, human
keywords: Gastric cancer; LINC00261; Metastasis; Prognosis