title: The Genetic Control of Grain Protein Content under Variable Nitrogen Supply in an Australian Wheat Mapping Population.
abstract: Genetic variation has been observed in both protein concentration in wheat grain and total protein content (protein yield). Here we describe the genetic analysis of variation for grain protein in response to nitrogen (N) supply and locate significant genomic regions controlling grain protein components in a spring wheat population. In total, six N use efficiency (NUE) field trials were carried out for the target traits in a sub-population of doubled haploid lines derived from a cross between two Australian varieties, RAC875 and Kukri, in Southern and Western Australia from 2011 to 2013. Twenty-four putative Quantitative Trait Loci (QTL) for protein-related traits were identified at high and low N supply and ten QTL were identified for the response to N for the traits studied. These loci accounted for a significant proportion of the overall effect of N supply. Several of the regions were co-localised with grain yield QTL and are promising targets for further investigation and selection in breeding programs.
authors.affiliation: Australian Centre for Plant Functional Genomics (ACPFG), The University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia.
authors.forename: Saba
authors.initials: S
authors.lastname: Mahjourimajd
authors.affiliation: School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.
authors.forename: Julian
authors.initials: J
authors.lastname: Taylor
authors.affiliation: Soil Science and Plant Nutrition M087, School of Earth and Environment, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
authors.forename: Zed
authors.initials: Z
authors.lastname: Rengel
authors.affiliation: Soil Science and Plant Nutrition M087, School of Earth and Environment, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
authors.forename: Hossein
authors.initials: H
authors.lastname: Khabaz-Saberi
authors.affiliation: Australian Grain Technologies, PMB1, Glen Osmond, SA 5064, Australia.|School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.
authors.forename: Haydn
authors.initials: H
authors.lastname: Kuchel
authors.affiliation: Australian Centre for Plant Functional Genomics (ACPFG), The University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Okamoto
authors.affiliation: School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.
authors.forename: Peter
authors.identifier: http://orcid.org/0000-0001-9494-400X
authors.initials: P
authors.lastname: Langridge
mesh_terms: D001315:Australia; D002874:Chromosome Mapping; D002980:Climate; D003433:Crosses, Genetic; D002523:Edible Grain / Q000235:genetics; D006238:Haploidy; D009584:Nitrogen / Q000494:pharmacology*; D010641:Phenotype; D010940:Plant Proteins / Q000378:metabolism; D040641:Quantitative Trait Loci / Q000235:genetics; D019655:Quantitative Trait, Heritable; D012639:Seeds / Q000187:drug effects / Q000235:genetics*; D012987:Soil / Q000737:chemistry; D014908:Triticum / Q000187:drug effects / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D012987:Soil; D009584:Nitrogen