title: Polymerase Chain Reaction Analysis of t(14;18) Junctional Regions in B-Cell Lymphomas.
abstract: The polymerase chain reaction (PCR) procedure was used for rapid and highly specific amplification of the t(14;18) bcl-2/JH DNA junctional regions in B-cell lymphomas. By using Taq-polymerase and relatively long oligonucleotide primers-a 33-mer for bcl-2 and an universal 25-mer complementary to the JH consensus sequence-the primer annealing and primer extension steps could be carried out at the same temperature (70°C), thus markedly reducing the reaction time and significantly improving the specificity of the reaction. The specificity of the amplification allowed visual identification of the bcl-2/JH PCR-products in ethidium bromide stained agarose gels. DNA-sequence analysis of PCR-amplified, previously uncharacterized t(14; 18) junctional regions, confirmed the specificity of this assay. Moreover, preliminary data show that the procedure is capable of documenting the presence of occult lymphoma cells in both the peripheral blood and bone marrow.
authors.affiliation: a Abteilung Hämatologie/Onkologie, Zentrum Innere Medizin Pathologisches Institut Klinikum Steglitz, Freie Universtität, Berlin, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Kneba
authors.affiliation: a Abteilung Hämatologie/Onkologie, Zentrum Innere Medizin Pathologisches Institut Klinikum Steglitz, Freie Universtität, Berlin, Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Eick
authors.affiliation: a Abteilung Hämatologie/Onkologie, Zentrum Innere Medizin Pathologisches Institut Klinikum Steglitz, Freie Universtität, Berlin, Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Willigeroth
authors.affiliation: a Abteilung Hämatologie/Onkologie, Zentrum Innere Medizin Pathologisches Institut Klinikum Steglitz, Freie Universtität, Berlin, Federal Republic of Germany.
authors.forename: I
authors.initials: I
authors.lastname: Bolz
authors.affiliation: b Klinikum Universität Göttingen, Max Planck Institute für Biophysikaliche Chemi eKlinikum Steglitz, Freie Universtität, Berlin, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Herbst
authors.affiliation: a Abteilung Hämatologie/Onkologie, Zentrum Innere Medizin Pathologisches Institut Klinikum Steglitz, Freie Universtität, Berlin, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Pott
authors.affiliation: a Abteilung Hämatologie/Onkologie, Zentrum Innere Medizin Pathologisches Institut Klinikum Steglitz, Freie Universtität, Berlin, Federal Republic of Germany.
authors.forename: G
authors.initials: G
authors.lastname: Mieskes
authors.affiliation: a Abteilung Hämatologie/Onkologie, Zentrum Innere Medizin Pathologisches Institut Klinikum Steglitz, Freie Universtität, Berlin, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Bergholz
authors.affiliation: c Gottingen and Institute fur Pathologien, Klinikum Steglitz, Freie Universtität, Berlin, Federal Republic of Germany.
authors.forename: W
authors.initials: W
authors.lastname: Brysch
authors.affiliation: c Gottingen and Institute fur Pathologien, Klinikum Steglitz, Freie Universtität, Berlin, Federal Republic of Germany.
authors.forename: K H
authors.initials: KH
authors.lastname: Schlingensiepen
authors.affiliation: b Klinikum Universität Göttingen, Max Planck Institute für Biophysikaliche Chemi eKlinikum Steglitz, Freie Universtität, Berlin, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Stein
authors.affiliation: a Abteilung Hämatologie/Onkologie, Zentrum Innere Medizin Pathologisches Institut Klinikum Steglitz, Freie Universtität, Berlin, Federal Republic of Germany.
authors.forename: G
authors.initials: G
authors.lastname: Krieger
keywords: B-cell lymphomas; DNA sequence; PCR; polymerase chain reaction; t(14;18)