title: In vivo and in vitro protective effects of omeprazole against neuropathic pain.
abstract: Apart from reducing the acid secretion, omeprazole inhibits activation of the nuclear factor-κB, release of inflammatory cytokines, and chemotaxis of neutrophils. These mechanisms prompted us to evaluate antineuropathic effect of omeprazole in the chronic constriction injury (CCI)-induced rat model of neuropathic pain and LPS mediated ROS-induced U-87 cells. Omeprazole at 50 mg/kg/day/oral for 14 days significantly reduced the intensity of neuropathic pain estimated as paw withdrawal latency, withdrawal pressure threshold and restored the motor nerve conduction velocity in the constricted nerve, when compared with respective groups. The histological findings revealed the protective effect of omeprazole against the CCI-induced damage. Omeprazole significantly decreased the levels of tumor necrosis factor (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) as compared to their respective control groups. It also reduced the oxidative stress by up regulating the SOD, catalase activity and decreasing MDA content. Similarly, in-vitro study, LPS mediated ROS-induced U-87 cells, omeprazole reduced the oxidative stress as well as the release of TNF-α, IL-1β and IL-6. Altogether, these results suggest that, neuroprotective effect of omeprazole is mediated through preventing release of proinflammatory cytokines, augmenting endogenous anti-oxidant defense system, and maintain the structural integrity of sciatic nerve from the CCI-induced structural damage and inflammatory changes.
authors.affiliation: Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist-Dhule-425405, Maharashtra, India.
authors.forename: Sanjay K
authors.initials: SK
authors.lastname: Chanchal
authors.affiliation: Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist-Dhule-425405, Maharashtra, India.
authors.forename: Umesh B
authors.initials: UB
authors.lastname: Mahajan
authors.affiliation: Department of Pathology, Gadag Institute of Medical Sciences, Bommanahalli, Gadag-582101, Karnataka, India.
authors.forename: Sumit
authors.initials: S
authors.lastname: Siddharth
authors.affiliation: Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist-Dhule-425405, Maharashtra, India.
authors.forename: Navyya
authors.initials: N
authors.lastname: Reddy
authors.affiliation: Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist-Dhule-425405, Maharashtra, India.
authors.forename: Sameer N
authors.initials: SN
authors.lastname: Goyal
authors.affiliation: Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist-Dhule-425405, Maharashtra, India.
authors.forename: Prakash H
authors.initials: PH
authors.lastname: Patil
authors.affiliation: Department of Pathology, Gadag Institute of Medical Sciences, Bommanahalli, Gadag-582101, Karnataka, India.
authors.forename: Basavaraj P
authors.initials: BP
authors.lastname: Bommanahalli
authors.affiliation: Cancer Biology Laboratory, KIIT School of Biotechnology, KIIT University, Bhubaneswar-721024, Odisha, India.
authors.forename: Chanakya N
authors.initials: CN
authors.lastname: Kundu
authors.affiliation: Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist-Dhule-425405, Maharashtra, India.
authors.forename: Chandragouda R
authors.initials: CR
authors.lastname: Patil
authors.affiliation: Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, Abu Dhabi 17666, United Arab Emirates.
authors.forename: Shreesh
authors.initials: S
authors.lastname: Ojha
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D003251:Constriction, Pathologic / Q000150:complications*; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007155:Immunologic Factors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D009437:Neuralgia / Q000188:drug therapy*; D018696:Neuroprotective Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D009853:Omeprazole / Q000008:administration & dosage* / Q000494:pharmacology*; D018384:Oxidative Stress; D017208:Rats, Wistar
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D009853:Omeprazole