title: Metabolomic profiles delineate the effect of Sanmiao wan on hyperuricemia in rats.
abstract: A serum metabolomic method based on ultra-high-performance liquid chromatography coupled with mass spectrometry was developed to characterize hyperuricemia-related metabolic profiles and delineate the mechanism of Sanmiao wan (SMW), a traditional Chinese medicine (TCM), in treating hyperuricemic rats. With partial least-squares discriminant analysis for classification and selection of biomarkers, 13 potential biomarkers in mouse serum were identified in the screen, primarily involved in purine metabolism, arginine and proline metabolism, citrate cycle, phenylalanine metabolism, tryptophan metabolism and glycerophospholipid metabolism. Taking these potential biomarkers as screening indexes, SMW could reverse the pathological process of hyperuricemia through partially regulating the perturbed metabolic pathway except for glycerophospholipid metabolism. Our results showed that a metabolomic approach offers a useful tool to identify hyperuricemia-related biomarkers and provides a new methodological cue for systematically dissecting the underlying efficacies and mechanisms of TCM in treating hyperuricemia.
authors.affiliation: Institution for Laboratory Medicine, Changshu, Jiangsu, 215500, China.
authors.forename: Tingwang
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Institution for Laboratory Medicine, Changshu, Jiangsu, 215500, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of Nephrology, Changhai Hospital, Shanghai, 200433, China.
authors.forename: Jiarong
authors.initials: J
authors.lastname: Ding
authors.affiliation: Department of Cardiothoracic Surgery, Changhai Hospital, Shanghai, 200433, China.
authors.forename: Guokun
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Cardiology, 117 Hospital of the Chinese People's Liberation Army, Hangzhou, Zhejiang, 310007, China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Cardiology, Changhai Hospital, Shanghai, 200433, China.
authors.forename: Suxuan
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Nephrology, Changhai Hospital, Shanghai, 200433, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D029044:Achyranthes / Q000737:chemistry; D000818:Animals; D031174:Atractylodes / Q000737:chemistry; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D020404:Glycerophospholipids / Q000378:metabolism; D033461:Hyperuricemia / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D016018:Least-Squares Analysis; D008297:Male; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics / Q000379:methods; D051379:Mice; D032102:Phellodendron / Q000737:chemistry; D051381:Rats
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D020404:Glycerophospholipids
keywords: Sanmiao wan; hyperuricemia; metabolomics; ultra-high-performance liquid chromatography-mass spectrometry