title: Systemic localization of seven major types of carbohydrates on cell membranes by dSTORM imaging.
abstract: Carbohydrates on the cell surface control intercellular interactions and play a vital role in various physiological processes. However, their systemic distribution patterns are poorly understood. Through the direct stochastic optical reconstruction microscopy (dSTORM) strategy, we systematically revealed that several types of representative carbohydrates are found in clustered states. Interestingly, the results from dual-color dSTORM imaging indicate that these carbohydrate clusters are prone to connect with one another and eventually form conjoined platforms where different functional glycoproteins aggregate (e.g., epidermal growth factor receptor, (EGFR) and band 3 protein). A thorough understanding of the ensemble distribution of carbohydrates on the cell surface paves the way for elucidating the structure-function relationship of cell membranes and the critical roles of carbohydrates in various physiological and pathological cell processes.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China.|University of Chinese Academy of Sciences, Beijing 100049, P.R. China.
authors.forename: Junling
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China.|University of Chinese Academy of Sciences, Beijing 100049, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China.|University of Chinese Academy of Sciences, Beijing 100049, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China.
authors.forename: Haijiao
authors.initials: H
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China.
authors.forename: Junguang
authors.initials: J
authors.lastname: Jiang
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China.
authors.forename: Hongda
authors.initials: H
authors.lastname: Wang
mesh_terms: D002232:Carbocyanines / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry* / Q000302:isolation & purification; D002462:Cell Membrane / Q000737:chemistry* / Q000235:genetics; D066246:ErbB Receptors / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008856:Microscopy, Fluorescence; D066329:Protein Aggregates / Q000235:genetics; D011485:Protein Binding; D015398:Signal Transduction
chemical_list: D002232:Carbocyanines; D002241:Carbohydrates; D005456:Fluorescent Dyes; D006023:Glycoproteins; D066329:Protein Aggregates; C512478:EGFR protein, human; D066246:ErbB Receptors