title: NDUFA4L2 expression predicts poor prognosis in clear cell renal cell carcinoma patients.
abstract: BACKGROUND
  NDUFA4L2 is overexpressed in VHL-deficient cell lines and neuroblastoma. The clinical significance of NDUFA4L2 in clear cell renal cell carcinoma (ccRCC) has not been well studied. Therefore, we evaluated the prognostic value of NDUFA4L2 in ccRCC patients.
  
  
  METHODS
  In our study, NDUFA4L2 expression in 86 cases of ccRCC and adjacent normal tissues was monitored by immunohistochemistry, semi-quantitative RT-PCR, and Western blot analyses. The relationship between NDUFA4L2 expression and the clinical features of ccRCC was assessed.
  
  
  RESULTS
  The results showed that NDUFA4L2 protein expression was found to be higher in ccRCC tissues 81.4% (70/86) than in normal tissues 26.7% (23/86) (p = 0.021). The average level of NDUFA4L2 mRNA expression was found to be 122.23 ± 6.018 and 21.34 ± 1.036 in ccRCC tissue and adjacent normal tissue (p < 0.001). NDUFA4L2 expression levels were correlated with some clinical features of ccRCC. Multivariate analysis showed NDUFA4L2 expression was an independent prognostic factor for ccRCC patients.
  
  
  CONCLUSIONS
  Our study has provided the significant clinical relevance of NDUFA4L2 in ccRCC and suggested that ccRCC patients with NDUFA4L2 overexpression may be suitable as a potential therapeutic target for ccRCC patients.
authors.affiliation: a Department of Urological Organ Transplantation , The Second Xiangya Hospital, Central South University , Changsha , China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: a Department of Urological Organ Transplantation , The Second Xiangya Hospital, Central South University , Changsha , China.
authors.forename: Gongbin
authors.initials: G
authors.lastname: Lan
authors.affiliation: a Department of Urological Organ Transplantation , The Second Xiangya Hospital, Central South University , Changsha , China.
authors.forename: Longkai
authors.initials: L
authors.lastname: Peng
authors.affiliation: a Department of Urological Organ Transplantation , The Second Xiangya Hospital, Central South University , Changsha , China.
authors.forename: Xubiao
authors.initials: X
authors.lastname: Xie
authors.affiliation: a Department of Urological Organ Transplantation , The Second Xiangya Hospital, Central South University , Changsha , China.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Peng
authors.affiliation: a Department of Urological Organ Transplantation , The Second Xiangya Hospital, Central South University , Changsha , China.
authors.forename: Shaojie
authors.initials: S
authors.lastname: Yu
authors.affiliation: a Department of Urological Organ Transplantation , The Second Xiangya Hospital, Central South University , Changsha , China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Department of Urological Organ Transplantation , The Second Xiangya Hospital, Central South University , Changsha , China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Tang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D002681:China; D018572:Disease-Free Survival; D003576:Electron Transport Complex IV / Q000235:genetics*; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D003576:Electron Transport Complex IV; C577147:NDUFA4 protein, human
keywords: Clear cell renal cell carcinoma; NDUFA4L2; biomarker; prognosis