title: Protective effect of naringin against ankylosing spondylitis via ossification, inflammation and oxidative stress in mice.
abstract: Naringin is an abundant flavanone in pomelo, grapefruit as well as lime and its variants, has been shown to exhibit certain antioxidative, anti-inflammatory, anti-cancer and hypoglycemic effects. The aim of the current study was to evaluate the protective effects of naringin against ankylosing spondylitis (AS) and to elucidate the potential underlying mechanism. Firstly, a mouse model of ankylosing spondylitis (AS) was established. Next, osteocalcin (OC), alkaline phosphatase (ALP) and triglyceride (TG) activity values, inflammatory factor and oxidative stress were evaluated in the AS mice. Then, the Janus kinase 2 (JAK2) and signal transducer and activator of transcription 3 (STAT3) protein expression levels in the AS mice were investigated using western blot analysis. The results showed that naringin increased OC, ALP and TG activity values in the AS mouse model. Furthermore, inflammatory factor and oxidative stress levels in the AS mice were restrained by treatment with naringin. Furthermore, JAK2 and STAT3 protein expression levels were reduced by treatment with naringin. In conclusion, the present results indicated that the protective effects of naringin against AS are exerted via the induction of ossification, suppression of inflammation and oxidative stress and the downregulation of JAK2/STAT3 in mice.
authors.affiliation: Department of Orthopedics, The Second Hospital Affiliated to Zhejiang University of Chinese Medicine, Hangzhou, Zhejiang 310005, P.R. China.
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, The Second Hospital Affiliated to Zhejiang University of Chinese Medicine, Hangzhou, Zhejiang 310005, P.R. China.
authors.forename: Lianguo
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, The Second Hospital Affiliated to Zhejiang University of Chinese Medicine, Hangzhou, Zhejiang 310005, P.R. China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Orthopedics, The Second Hospital Affiliated to Zhejiang University of Chinese Medicine, Hangzhou, Zhejiang 310005, P.R. China.
authors.forename: Fengqing
authors.initials: F
authors.lastname: Wu
keywords: ankylosing spondylitis; inflammation; naringin; ossification; oxidative stress