title: Corilagin ameliorates schistosomiasis hepatic fibrosis through regulating IL-13 associated signal pathway in vitro and in vivo.
abstract: Interleukin (IL)-13-associated signal pathway plays an important role in schistosomiasis hepatic fibrosis. In this study we tried to investigate the effects of corilagin to ameliorate schistosomiasis hepatic fibrosis through regulating IL-13-associated signal pathway in vitro and in vivo. Cellular model was set up with hepatic stellate cells-T6 cells stimulated by rIL-13 and male Balb/c mice were infected with Schistosoma japonicum cercariaeas as animal model. Liver histological changes were observed with haematoxylin and eosin staining. Masson staining was employed to observe the change of egg granulomas. Expression of Col (collagen) and Col III were examined with Immunohistochemistry. Western bolt was employed to detect the JAK-1 and IL13Rα1 proteins. The mRNA expression of Col I, Col III, IL-13, JAK-1 and IL13Rα1 were tested by quantitative polymerase chain reaction. As a result, less inflammatory changes were found in all corilagin groups compared with model group and praziquantel group. The mRNA levels of Col I, Col III, IL-13, JAK-1 and IL13Rα1 were significantly decreased after corilagin intervention (P < 0·01). JAK-1 and IL-13Rα1 protein levels were also greatly decreased in the corilagin groups (P < 0·01). In conclusion, corilagin could ameliorate schistosomiasis hepatic fibrosis by down-regulating the expression of IL-13 and signal molecules in IL-13 pathway.
authors.affiliation: Department of Infectious Diseases,Institute of Infection and Immunology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,P.R. China.
authors.forename: Hua-Rong
authors.initials: HR
authors.lastname: Li
authors.affiliation: Department of Infectious Diseases,Renmin Hospital,Hubei University of Medicine,Shiyan 442000,P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Neurology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,P.R. China.
authors.forename: Man
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Infectious Diseases,Institute of Infection and Immunology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,P.R. China.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery,The 2nd Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,P.R. China.
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.affiliation: Department of Neurology,Wuhan General Hospital of Guangzhou Military Command,Wuhan 430070,P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.affiliation: Central Lab,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,P.R. China.
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Dong
authors.affiliation: Department of Neurology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,P.R. China.
authors.forename: Yuan-Jin
authors.initials: YJ
authors.lastname: Guo
authors.affiliation: Department of Infectious Diseases,Institute of Infection and Immunology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D003094:Collagen / Q000032:analysis; D004195:Disease Models, Animal; D005765:Gastrointestinal Agents / Q000008:administration & dosage*; D020869:Gene Expression Profiling; D005960:Glucosides / Q000008:administration & dosage*; D006651:Histocytochemistry; D047348:Hydrolyzable Tannins / Q000008:administration & dosage*; D007150:Immunohistochemistry; D018793:Interleukin-13 / Q000378:metabolism*; D053661:Interleukin-13 Receptor alpha1 Subunit / Q000032:analysis; D053613:Janus Kinase 1 / Q000032:analysis; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000473:pathology* / Q000628:therapy*; D008807:Mice, Inbred BALB C; D008853:Microscopy; D008954:Models, Biological; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D012552:Schistosomiasis / Q000150:complications*; D015398:Signal Transduction; D016896:Treatment Outcome
chemical_list: D005765:Gastrointestinal Agents; D005960:Glucosides; D047348:Hydrolyzable Tannins; C508183:Il13ra1 protein, mouse; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; C049096:corilagin; D003094:Collagen; C507905:Jak1 protein, mouse; D053613:Janus Kinase 1
keywords: IL-13; schistosomiasis; corilagin; liver fibrosis