title: Lipoplexes Strengthened by Anionic Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids and Anionic Polymers.
abstract: RNA interference is an invaluable tool in biology to specifically silence a given gene. Synthetic duplexes of RNA oligonucleotides are widely used to induce mRNA degradation in cultured cells or in whole organisms. They have to be vectorized to reach their target site. Here, we describe the preparation of highly efficient siRNA vectors based on cationic liposomes and polyanionic polymers and their application in cultured cells to silence reporter and/or endogenous genes.
authors.affiliation: Dep Faculdade de Farmácia, Universidade Federal de Minas Gerais, avenida Presidente Antônio Carlos, 6627, 31270-901, Belo Horizonte, Brazil.
authors.forename: Danielle Campiol
authors.initials: DC
authors.lastname: Arruda
authors.affiliation: UTCBS, CNRS UMR8258, INSERM U1022, Université Paris Descartes, Chimie ParisTech, 75006, Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Schlegel
authors.affiliation: UTCBS, CNRS UMR8258, INSERM U1022, Université Paris Descartes, Chimie ParisTech, 75006, Paris, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Bigey
authors.affiliation: UTCBS, CNRS UMR8258, INSERM U1022, Université Paris Descartes, Chimie ParisTech, 75006, Paris, France. virginie.escriou@parisdescartes.fr.
authors.forename: Virginie
authors.initials: V
authors.lastname: Escriou
mesh_terms: D000818:Animals; D000838:Anions / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D045744:Cell Line, Tumor; D018014:Gene Transfer Techniques; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes; D051379:Mice; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics*
chemical_list: D000838:Anions; D002412:Cations; D008055:Lipids; D008081:Liposomes; D011108:Polymers; D034741:RNA, Small Interfering
keywords: Anionic polymer; Cationic lipid; Lipoplexes; RNA interference; siRNA delivery