title: A novel strategy combining magnetic particle hyperthermia pulses with enhanced performance binary ferrite carriers for effective in vitro manipulation of primary human osteogenic sarcoma cells.
abstract: The present study examines the heating efficiency of a combination of manganese or cobalt ferrites in a binary (Co- or Mn-) ferrite nanoparticle form with magnetite, covered with citric acid to improve biocompatibility. The nanoparticle synthesis is based on the aqueous co-precipitation of proper salts, a facile, low-cost, environmentally friendly and high yield synthetic approach. By detailed structural and magnetic characterisation, the direct influence of structural and magnetic features on magnetic hyperthermia concludes to optimum heating efficiency. At a second stage, best performing magnetic nanoparticles undergo in vitro testing in three cell lines: one cancer cell line and two reference healthy cell lines. Both binary ferrite (MnFe2O4/Fe3O4 and CoFe2O4/Fe3O4) appear to be internalised and well tolerated by the cells while a versatile hyperthermia protocol is attempted in an effort to further improve their in vitro performance. Within this protocol, hyperthermia sequences are split in two runs with an intermediate 48 h time interval cell incubation stage while in each run a variable field mode (single or multiple pulses) is applied. Single-pulse field mode represents a typical hyperthermia application scheme where cells undergo the thermal shock continuously. On the other hand multiple-pulses mode refers to multiple, much shorter in duration AC field changes (field ON/OFFs), at each hyperthermia run, resulting eventually in high heating rate and much more harmful cell treatment. Consequently, we propose a novel series of improved performance heat mediators based on ferrite structures which show maximum efficiency at cancer cells when combined with a versatile multiple-pulse hyperthermia module.
authors.affiliation: a Department of Physics , Aristotle University of Thessaloniki , Thessaloniki , Greece ;
authors.forename: Antonios
authors.initials: A
authors.lastname: Makridis
authors.affiliation: b Laboratory of Clinical and Molecular Endocrinology, 1st Department of Internal Medicine , AHEPA University Hospital, Aristotle University of Thessaloniki , Thessaloniki , Greece.
authors.forename: Magdalini
authors.initials: M
authors.lastname: Tziomaki
authors.affiliation: b Laboratory of Clinical and Molecular Endocrinology, 1st Department of Internal Medicine , AHEPA University Hospital, Aristotle University of Thessaloniki , Thessaloniki , Greece.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Topouridou
authors.affiliation: b Laboratory of Clinical and Molecular Endocrinology, 1st Department of Internal Medicine , AHEPA University Hospital, Aristotle University of Thessaloniki , Thessaloniki , Greece.
authors.forename: Maria P
authors.initials: MP
authors.lastname: Yavropoulou
authors.affiliation: b Laboratory of Clinical and Molecular Endocrinology, 1st Department of Internal Medicine , AHEPA University Hospital, Aristotle University of Thessaloniki , Thessaloniki , Greece.
authors.forename: John G
authors.initials: JG
authors.lastname: Yovos
authors.affiliation: a Department of Physics , Aristotle University of Thessaloniki , Thessaloniki , Greece ;
authors.forename: Orestis
authors.initials: O
authors.lastname: Kalogirou
authors.affiliation: a Department of Physics , Aristotle University of Thessaloniki , Thessaloniki , Greece ;
authors.forename: Theodoros
authors.initials: T
authors.lastname: Samaras
authors.affiliation: a Department of Physics , Aristotle University of Thessaloniki , Thessaloniki , Greece ;
authors.forename: Mavroeidis
authors.initials: M
authors.lastname: Angelakeris
mesh_terms: D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D012516:Osteosarcoma / Q000737:chemistry*; D013696:Temperature
chemical_list: D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C001215:ferrite
keywords: Magnetic particle hyperthermia; magnetic nanoparticles; multiple-pulses AMF; thermal dose