title: Lateral flow biosensor for multiplex detection of nitrofuran metabolites based on functionalized magnetic beads.
abstract: The use of potential mutagenic nitrofuran antibiotic in food animal production has been banned world-wide. Common methods for nitrofuran detection involve complex extraction procedures. In the present study, magnetic beads functionalized with antibody against nitrofuran derivative were used as both the extraction and color developing media in lateral flow biosensor. Derivatization reagent carboxybenzaldehyde is firstly modified with ractopamine. After reaction with nitrofuran metabolites, the resultant molecule has two functional groups: the metabolite moiety and the ractopamine moiety. Metabolite moiety is captured by the antibody that is coated on magnetic beads. This duplex is then loaded onto biosensor and ractopamine moiety is further captured by the antibody immobilized on the test zone of nitrocellulose membrane. Without tedious organic reagent-based extraction procedure, this biosensor was capable of visually detecting four metabolites simultaneously with a detection limit of 0.1 μg/L. No cross-reactivity was observed in the presence of 50 μg/L interferential components. Graphical abstract Derivatization of nitrofuran metabolites (AHD, AOZ, SEM, or AMOZ) and LFA detection of the derivative products.
authors.affiliation: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Lu
authors.affiliation: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China.|Institute of Environmental and Food Safety, Wuhan Academy of Agricultural Science and Technology, Wuhan, Hubei, 430000, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liang
authors.affiliation: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China.
authors.forename: Jianghong
authors.initials: J
authors.lastname: Dong
authors.affiliation: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China. fangjnu@126.com.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China. lzeng8@126.com.|Institute of Environmental and Food Safety, Wuhan Academy of Agricultural Science and Technology, Wuhan, Hubei, 430000, China. lzeng8@126.com.
authors.forename: Lingwen
authors.initials: L
authors.lastname: Zeng
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006041:Goats; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry; D051379:Mice; D009581:Nitrofurans / Q000032:analysis* / Q000378:metabolism; D010627:Phenethylamines / Q000032:analysis; D011934:Reagent Strips / Q000032:analysis*
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D009581:Nitrofurans; D010627:Phenethylamines; D011934:Reagent Strips; C060897:ractopamine
keywords: Fast pre-treatment; Lateral flow biosensor; Magnetic separation; Nitrofuran metabolites