title: Screening of Living Kidney Donors for Genetic Diseases Using a Comprehensive Genetic Testing Strategy.
abstract: Related living kidney donors (LKDs) are at higher risk of end-stage renal disease (ESRD) compared with unrelated LKDs. A genetic panel was developed to screen 115 genes associated with renal diseases. We used this panel to screen six negative controls, four transplant candidates with presumed genetic renal disease and six related LKDs. After removing common variants, pathogenicity was predicted using six algorithms to score genetic variants based on conservation and function. All variants were evaluated in the context of patient phenotype and clinical data. We identified causal variants in three of the four transplant candidates. Two patients with a family history of autosomal dominant polycystic kidney disease segregated variants in PKD1. These findings excluded genetic risk in three of four relatives accepted as potential LKDs. A third patient with an atypical history for Alport syndrome had a splice site mutation in COL4A5. This pathogenic variant was excluded in a sibling accepted as an LKD. In another patient with a strong family history of ESRD, a negative genetic screen combined with negative comparative genomic hybridization in the recipient facilitated counseling of the related donor. This genetic renal disease panel will allow rapid, efficient and cost-effective evaluation of related LKDs.
authors.affiliation: Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA.|Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA.|VA Medical Center, Iowa City, IA.
authors.forename: C P
authors.initials: CP
authors.lastname: Thomas
authors.affiliation: Iowa Institute of Human Genetics, Carver College of Medicine, University of Iowa, Iowa City, IA.
authors.forename: M A
authors.initials: MA
authors.lastname: Mansilla
authors.affiliation: Iowa Institute of Human Genetics, Carver College of Medicine, University of Iowa, Iowa City, IA.
authors.forename: R
authors.initials: R
authors.lastname: Sompallae
authors.affiliation: Iowa Institute of Human Genetics, Carver College of Medicine, University of Iowa, Iowa City, IA.
authors.forename: S O
authors.initials: SO
authors.lastname: Mason
authors.affiliation: Iowa Institute of Human Genetics, Carver College of Medicine, University of Iowa, Iowa City, IA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Nishimura
authors.affiliation: Iowa Institute of Human Genetics, Carver College of Medicine, University of Iowa, Iowa City, IA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Kimble
authors.affiliation: Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA.|Iowa Institute of Human Genetics, Carver College of Medicine, University of Iowa, Iowa City, IA.
authors.forename: C A
authors.initials: CA
authors.lastname: Campbell
authors.affiliation: Iowa Institute of Human Genetics, Carver College of Medicine, University of Iowa, Iowa City, IA.|Department of Pharmacology, Carver College of Medicine, University of Iowa, Iowa City, IA.
authors.forename: A E
authors.initials: AE
authors.lastname: Kwitek
authors.affiliation: Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA.|Interdisciplinary Program in Genetics, University of Iowa, Iowa City, IA.|The Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA.
authors.forename: B W
authors.initials: BW
authors.lastname: Darbro
authors.affiliation: Department of Surgery, Division of Transplant Surgery, Carver College of Medicine, University of Iowa, Iowa City, IA.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Stewart
authors.affiliation: Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA.|Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA.|Iowa Institute of Human Genetics, Carver College of Medicine, University of Iowa, Iowa City, IA.|Interdisciplinary Program in Genetics, University of Iowa, Iowa City, IA.|Department of Otorhinolaryngology, Carver College of Medicine, University of Iowa, Iowa City, IA.
authors.forename: R J H
authors.initials: RJ
authors.lastname: Smith
mesh_terms: D000328:Adult; D005260:Female; D005819:Genetic Markers; D005820:Genetic Testing / Q000379:methods*; D005923:Glomerulosclerosis, Focal Segmental / Q000175:diagnosis* / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D016030:Kidney Transplantation; D019520:Living Donors; D008297:Male; D008403:Mass Screening; D008875:Middle Aged; D009154:Mutation; D010375:Pedigree; D016891:Polycystic Kidney, Autosomal Dominant / Q000175:diagnosis* / Q000235:genetics; D051436:Renal Insufficiency, Chronic / Q000175:diagnosis* / Q000235:genetics; D055815:Young Adult
chemical_list: D005819:Genetic Markers
keywords: clinical decision-making; clinical research/practice; diagnostic techniques and imaging; donors and donation: living; genetics; kidney transplantation/nephrology; kidney transplantation: living donor; translational research/science