title: Highly Efficient Neural Differentiation of CD34-Positive Hair-Follicle-Associated Pluripotent Stem Cells Induced by Retinoic Acid and Serum-Free Medium.
abstract: Neural differentiation of hair-follicle-associated pluripotent (HAP) stem cells residing in the bulge area is a promising autologous source for stem cell therapy. In the present chapter, we describe the identification and enrichment of CD34(+) HAP stem cells by magnetic-activated cell sorting (MACS), and induce them to differentiate into neuronal and glial cells using defined neural-induction media. The different neural cell populations arising during in vitro differentiation from HAP stem cells are characterized by reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry assay.
authors.affiliation: Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sagha
authors.affiliation: Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. nowruz30@gmail.com.
authors.forename: Nowruz
authors.initials: N
authors.lastname: Najafzadeh
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D015415:Biomarkers; D002454:Cell Differentiation / Q000187:drug effects*; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005260:Female; D018859:Hair Follicle / Q000166:cytology*; D007150:Immunohistochemistry; D016130:Immunophenotyping; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D016895:Culture Media, Serum-Free; D014212:Tretinoin
keywords: CD34+ hair-follicle-associated-pluripotent (HAP) stem cell; Hair follicle; Magnetic-activated cell sorting; Neural differentiation; Stem cells