title: [Research on biological and genetic characteristics of human placenta mesenchymal stem cells cultured in vitro].
abstract: OBJECTIVE
  To investigate the biological characteristics and genetic features of human placenta mesenchymal stem cells (hPA-MSCs) cultured in vitro in order to assess its safety for clinical use.
  
  
  METHODS
  The shapes of the 1st, 3rd, 5th, 7th, 10th, 13th, 17th and 20th generation hPA-MSCs cultured in vitro using serum-free culture medium were observed. Their cell cycle, cell surface markers, and karyotype were analyzed, and relevant genes and cytokines were measured.
  
  
  RESULTS
  The shape of hPA-MSCs has remained as fusiform or short fusiform, and there was no significant change. About 93% of hPA-MSCs cells were in G0/G1 phase and remained stable. No obvious chromosomal translocation, loss or inversion was noted by karyotyping analysis. Cytokines expression level remained stable. Related gene expression level as a whole was on the decline, but the gene expression level of the first five generations showed very slight variations, with genetic characteristics remaining stable.
  
  
  CONCLUSIONS
  The hPA-MSCs cultured in vitro with serum-free medium has retained stable in the first five generations.
authors.affiliation: Chengdu Qingke Biotechnology Co. Ltd., Chengdu, Sichuan 610094, China; Chengdu Zhongchuang Qingke Medical Test Center, Chengdu, Sichuan 610094, China; Department of Biochemistry and Molecular Biology, College of Basic and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China. Emails: zr1223@126.com; haiq1984@163.com.
authors.forename: Dongming
authors.initials: D
authors.lastname: Zheng
authors.forename: Xiaorui
authors.initials: X
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.forename: Yin
authors.initials: Y
authors.lastname: Deng
authors.forename: Jianglin
authors.initials: J
authors.lastname: Li
authors.forename: Shuangqing
authors.initials: S
authors.lastname: Cen
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Quan
authors.initials: Q
authors.lastname: Hai
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis; D005260:Female; D006801:Humans; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy
chemical_list: D016207:Cytokines