title: Post-Synthetic Defucosylation of AGP by Aspergillus nidulans α-1,2-Fucosidase Expressed in Arabidopsis Apoplast Induces Compensatory Upregulation of α-1,2-Fucosyltransferases.
abstract: Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant. Besides mechanical function providing cell shape, cell walls participate in intercellular communication, defense during plant-microbe interactions, and plant growth. The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes. Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations. Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes. These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.
authors.affiliation: Roy J Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 3212 MMB, Ames, IA, United States of America.|Department of Plant Pathology and Microbiology, Iowa State University, 219 Bessey Hall, Ames, IA, United States of America.
authors.forename: Gennady V
authors.initials: GV
authors.lastname: Pogorelko
authors.affiliation: Roy J Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 3212 MMB, Ames, IA, United States of America.
authors.forename: Nathan T
authors.initials: NT
authors.lastname: Reem
authors.affiliation: Roy J Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 3212 MMB, Ames, IA, United States of America.
authors.forename: Zachary T
authors.initials: ZT
authors.lastname: Young
authors.affiliation: Roy J Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 3212 MMB, Ames, IA, United States of America.
authors.forename: Lauran
authors.initials: L
authors.lastname: Chambers
authors.affiliation: Roy J Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 3212 MMB, Ames, IA, United States of America.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Zabotina
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins; D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D004789:Enzyme Activation; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005786:Gene Expression Regulation; D009088:Mucoproteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins; D005644:alpha-L-Fucosidase / Q000378:metabolism; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D029681:Arabidopsis Proteins; D005647:Fucosyltransferases; D009088:Mucoproteins; D010940:Plant Proteins; D011134:Polysaccharides; D011994:Recombinant Proteins; D005644:alpha-L-Fucosidase; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C006619:arabinogalactan proteins