title: Quantification of Ethylene Production in Tomato Leaves Infected byXanthomonas euvesicatoria.
abstract: Ethylene is a gaseous plant hormone controlling fruit ripening, flower opening, leaf senescence as well as abscission, and disease symptom development. Ethylene plays a critical role in the bacterial pathogenXanthomonas euvesicatoria(X. euvesicatoria)-elicited symptom development in tomato. This protocol describes the measurement of ethylene gas produced by tomato leaves infected withX. euvesicatoria. Infected leaflets are placed in a glass tube for 30 min without sealing. The glass tubes are then capped with a septa stopper, and incubated for an hour. A 1 ml gas sample is removed from the tube using a syringe and then injected into a gas chromatograph to quantify ethylene gas levels. This protocol will be applicable for other plants with other pathogens with modifications.
authors.affiliation: Department of Biology, Stanford University, Stanford, USA.
authors.forename: Jung-Gun
authors.initials: JG
authors.lastname: Kim
authors.affiliation: Department of Biology, Stanford University, Stanford, USA.
authors.forename: William
authors.initials: W
authors.lastname: Stork
authors.affiliation: Department of Biology, Stanford University, Stanford, USA.
authors.forename: Mary Beth
authors.initials: MB
authors.lastname: Mudgett