title: Embedding complementary imaging data in laser scanning microscopy micrographs by reversible watermarking.
abstract: Complementary laser scanning microscopy micrographs are considered as pairs consisting in a master image (MI) and a slave image (SI), the latter with potential for facilitating the interpretation of the MI. We propose a strategy based on reversible watermarking for embedding a lossy compressed version of the SI into the MI. The use of reversible watermarking ensures the exact recovery of the host image. By storing and/or transmitting the watermarked MI in a single file, the information contained in both images that constitute the pair is made available to a potential end-user, which simplifies data association and transfer. Examples are presented using support images collected by two complementary techniques, confocal scanning laser microscopy and transmission laser scanning microscopy, on Hematoxylin and Eosin stained tissue fragments. A strategy for minimizing the watermarking distortions of the MI, while preserving the content of the SI, is discussed in detail.
authors.affiliation: Electrical Engineering Department, Valahia University of Targoviste, Bd. Carol I, nr. 2, Targoviste, Romania.
authors.forename: Ioan-Catalin
authors.initials: IC
authors.lastname: Dragoi
authors.affiliation: Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei Bvd., Bucharest, Romania; stefan.stanciu@cmmip-upb.org.
authors.forename: Stefan G
authors.initials: SG
authors.lastname: Stanciu
authors.affiliation: Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei Bvd., Bucharest, Romania.
authors.forename: Radu
authors.initials: R
authors.lastname: Hristu
authors.affiliation: Electrical Engineering Department, Valahia University of Targoviste, Bd. Carol I, nr. 2, Targoviste, Romania.
authors.forename: Henri-George
authors.initials: HG
authors.lastname: Coanda
authors.affiliation: Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei Bvd., Bucharest, Romania.
authors.forename: Denis E
authors.initials: DE
authors.lastname: Tranca
authors.affiliation: Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei Bvd., Bucharest, Romania; Department of Plastic Surgery and Reconstructive Microsurgery, Clinical Emergency Hospital of Bucharest, 8 Calea Floreasca St., Bucharest, Romania.
authors.forename: Marius
authors.initials: M
authors.lastname: Popescu
authors.affiliation: Electrical Engineering Department, Valahia University of Targoviste, Bd. Carol I, nr. 2, Targoviste, Romania; dinu.coltuc@valahia.ro.
authors.forename: Dinu
authors.initials: D
authors.lastname: Coltuc
keywords: (100.2000) Digital image processing; (110.3010) Image reconstruction techniques; (170.1790) Confocal microscopy; (350.2660) Fusion