title: Molecular detection of virulence genes in Pseudomonas aeruginosa isolated from children with Cystic Fibrosis and burn wounds in Iran.
abstract: Pseudomonas aeruginosa possesses various virulence factors which contribute to the bacterial invasion and toxicity. Moreover, children suffered from Cystic Fibrosis (CF) and burn wounds are at a high risk of various bacterial infections. The aim of this study was to determine the prevalence of virulent genes in P. aeruginosa isolated from children with CF and burn wounds and comparing their virulence genes to figure out the role of every virulent factor in the infections. P. aeruginosa were isolated from sputum, oropharyngeal swabs, and broncho-alveolar lavage (BAL) specimens from CF and burn wounds between June 2013 and June 2014 in Tehran's hospitals. Bacterial genomic DNAs were extracted and uniplex, duplex and multiplex PCR were performed for detection of toxA, algD and plcN, exoS, lasB, plcH genes, respectively. The prevalence rate of virulence genes in P. aeruginosa isolated from CF was; toxA (63.1%), algD (64.6%), plcH (87.7%), plcN (60%), lasB (95.4%) and exoS (70.8%) and virulence genes in P. aeruginosa from burn patients were: toxA (36.9%), algD (70.1%), plcH (79%), plcN (63.1%), lasB (82%) and exoS (21.1%). The prevalence of three virulent genes in P. aeruginosa was higher in CF comparing to burn wound infections. We found that the number of toxA, lasB and exoS were significantly higher in the bacteria which were isolated from children with CF. This finding shows that these virulence factors play an important role in CF infections by P. aeroginosa.
authors.affiliation: Dept. of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Faraji
authors.affiliation: Dept. of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran. Electronic address: Mahzounieh@vet.sku.ac.ir.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Mahzounieh
authors.affiliation: Dept. of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
authors.forename: Azizollah
authors.initials: A
authors.lastname: Ebrahimi
authors.affiliation: Pediatric Infections Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Fallah
authors.affiliation: Department of Microbiology, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Omid
authors.initials: O
authors.lastname: Teymournejad
authors.affiliation: Pediatric Infections Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Behnaz
authors.initials: B
authors.lastname: Lajevardi
mesh_terms: D002056:Burns / Q000150:complications*; D002648:Child; D003550:Cystic Fibrosis / Q000150:complications*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D007492:Iran; D060885:Multiplex Polymerase Chain Reaction; D015995:Prevalence; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D037521:Virulence Factors / Q000032:analysis / Q000235:genetics*; D014946:Wound Infection / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D037521:Virulence Factors
keywords: Burn patients; Children; Cystic fibrosis; Pseudomonas aeruginosa; Virulence factors