title: [THE APPLICATION OF DOT-TECHNIQUE FOR DETECTING ANTIGENS OF ADENOVIRUS IN CLINICAL SAMPLES].
abstract: The article substantiates possibility of application of point enzyme-linked immunosorbent assay (dot-technique) for detecting viral antigens in samples from patients. To diagnose adenovirus infection conjugate of virus-specific monoclonal antibodies and peroxidase of horse-radish were used The chromatographic rectification of conjugate from free peroxidase permits diminishing background coloring of nitrocellulose membrane and therefore to increase sensitivity. The application of direct conjugates on the basis of virus-specific monoclonal antibodies increases specifcity of dot-technique and significantly shortens time period of analysis. As in case of application of direct conjugates on the basis of polyclonal serum, samples from patients require preliminary processing with detergent for preventing non-specific reactions. The dot-technique demonstrates good coincidence with data of polymerase chain reaction and after clinical trials it can be used in diagnostic of human viral infections.
authors.forename: I A
authors.initials: IA
authors.lastname: Ivanova
authors.forename: M M
authors.initials: MM
authors.lastname: Pisareva
authors.forename: G F
authors.initials: GF
authors.lastname: Leontieva
authors.forename: T D
authors.initials: TD
authors.lastname: Smirnova
authors.forename: E V
authors.initials: EV
authors.lastname: Sorokin
authors.forename: I V
authors.initials: IV
authors.lastname: Amosova
authors.forename: E R
authors.initials: ER
authors.lastname: Petrova
authors.forename: A A
authors.initials: AA
authors.lastname: Shaldjian
authors.forename: A A
authors.initials: AA
authors.lastname: Sirosh
authors.forename: V G
authors.initials: VG
authors.lastname: Maiorova
mesh_terms: D000256:Adenoviridae / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000914:Antibodies, Viral / Q000737:chemistry*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000097:blood*; D006385:Hemagglutination Inhibition Tests; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D018796:Immunoconjugates / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D018796:Immunoconjugates; D006735:Horseradish Peroxidase