title: Lipoplexes from Non-viral Cationic Vectors: DOTAP-DOPE Liposomes and Gemini Micelles.
abstract: This chapter describes the topic of gene therapy based on colloidal drug delivery, as an alternative to the use of viral carriers. Non-viral vectors are promising transfection agents and do not suffer from limitations related to toxicity and immunogenic effects. In particular, lipid-based aggregates are generally considered biocompatible and versatile nanocarriers whose composition can be designed to include a cationic molecule which ensures strong interaction with nucleic acid. Herein the main issues related to complex formation and in vitro administration are illustrated with key examples, such as liposome-DNA plasmid (pDNA) association and micelles-siRNA complexes.
authors.affiliation: Department of Chemistry "Ugo Shiff" & CSGI, University of Florence, via della Lastruccia 3, 50019, Sesto Fiorentino, Firenze, Italy. sarafalsini@gmail.com.
authors.forename: Sara
authors.initials: S
authors.lastname: Falsini
authors.affiliation: Dipartimento di Scienze della Terra, Università di Firenze, Via La Pira 4, 50121, Florence, Italy.
authors.forename: Sandra
authors.initials: S
authors.lastname: Ristori
mesh_terms: D002412:Cations / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry*; D015316:Genetic Therapy; D057809:HEK293 Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology; D008823:Micelles; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics; D014162:Transfection
chemical_list: D002412:Cations; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D008823:Micelles; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D034741:RNA, Small Interfering; C094877:dioleoyl phosphatidylethanolamine; D004247:DNA; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane
keywords: Cationic micelles; Gene therapy; Lipoplex; Plasmid transfection; siRNA