title: Reassortment among picobirnaviruses found in wolves.
abstract: We conducted a viral metagenomics study in diarrheic free-ranging wolves in Portugal, revealing for the first time the presence of reassortant picobirnaviruses. These viruses shared identical capsid segments together with diverse RNA-dependent RNA polymerase segments. Even though causality between these picobirnaviruses and diarrhea could not be established, the study nonetheless confirms for the first time that wolves are a potential reservoir for picobirnaviruses, which might play a role as enteric pathogens.
authors.affiliation: Laboratory of Viral Metagenomics, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, University of Leuven, Leuven, Belgium.|Laboratory for Clinical Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, University of Leuven, Leuven, Belgium.
authors.forename: Nádia
authors.initials: N
authors.lastname: Conceição-Neto
authors.affiliation: Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal.|CIBIO/InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal.
authors.forename: João Rodrigo
authors.initials: JR
authors.lastname: Mesquita
authors.affiliation: Laboratory of Viral Metagenomics, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, University of Leuven, Leuven, Belgium.
authors.forename: Mark
authors.initials: M
authors.lastname: Zeller
authors.affiliation: Laboratory of Viral Metagenomics, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, University of Leuven, Leuven, Belgium.|Laboratory for Clinical Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, University of Leuven, Leuven, Belgium.
authors.forename: Claude Kwe
authors.initials: CK
authors.lastname: Yinda
authors.affiliation: CIBIO/InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal.
authors.forename: Francisco
authors.initials: F
authors.lastname: Álvares
authors.affiliation: cE3c, Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal.|Grupo Lobo, Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal.
authors.forename: Sara
authors.initials: S
authors.lastname: Roque
authors.affiliation: cE3c, Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal.|Grupo Lobo, Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal.
authors.forename: Francisco
authors.initials: F
authors.lastname: Petrucci-Fonseca
authors.affiliation: CIBIO/InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal.|Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007, Porto, Portugal.
authors.forename: Raquel
authors.initials: R
authors.lastname: Godinho
authors.affiliation: Laboratory of Viral Metagenomics, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, University of Leuven, Leuven, Belgium.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Heylen
authors.affiliation: Laboratory for Clinical Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, University of Leuven, Leuven, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Van Ranst
authors.affiliation: Laboratory of Viral Metagenomics, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, University of Leuven, Leuven, Belgium. jelle.matthijnssens@kuleuven.be.
authors.forename: Jelle
authors.initials: J
authors.lastname: Matthijnssens
mesh_terms: D000818:Animals; D014644:Genetic Variation; D056186:Metagenomics; D018708:Picobirnavirus / Q000235:genetics* / Q000302:isolation & purification; D011174:Portugal; D012327:RNA Virus Infections / Q000662:veterinary* / Q000821:virology; D016865:Reassortant Viruses / Q000235:genetics* / Q000302:isolation & purification; D019596:Wolves / Q000821:virology*