title: Contrasting Potato Foliage and Tuber Defense Mechanisms against the Late Blight Pathogen Phytophthora infestans.
abstract: The late blight pathogen Phytophthora infestans can attack both potato foliage and tubers. When inoculated with P. infestans, foliage of nontransformed 'Russet Burbank' (WT) develops late blight disease while that of transgenic 'Russet Burbank' line SP2211 (+RB) does not. We compared the foliar transcriptome responses of these two lines to P. infestans inoculation using an RNA-seq approach. A total of 515 million paired end RNA-seq reads were generated, representing the transcription of 29,970 genes. We also compared the differences and similarities of defense mechanisms against P. infestans in potato foliage and tubers. Differentially expressed genes, gene groups and ontology bins were identified to show similarities and differences in foliage and tuber defense mechanisms. Our results suggest that R gene dosage and shared biochemical pathways (such as ethylene and stress bins) contribute to RB-mediated incompatible potato-P. infestans interactions in both the foliage and tubers. Certain ontology bins such as cell wall and lipid metabolisms are potentially organ-specific.
authors.affiliation: Department of Plant Pathology, University of Minnesota, St Paul, Minnesota, United States of America.|Department of Agronomy and Plant Genetics, University of Minnesota, St Paul, Minnesota, United States of America.
authors.forename: Liangliang
authors.identifier: http://orcid.org/0000-0002-3864-0631
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Plant Pathology, University of Minnesota, St Paul, Minnesota, United States of America.|Stakman-Borlaug Center for Sustainable Plant Health, University of Minnesota, St. Paul, Minnesota, United States of America.
authors.forename: James M
authors.initials: JM
authors.lastname: Bradeen
mesh_terms: D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D060467:Disease Resistance / Q000235:genetics; D020869:Gene Expression Profiling; D063990:Gene Ontology; D018745:Genome, Plant; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D006790:Host-Parasite Interactions / Q000235:genetics; D010641:Phenotype; D055750:Phytophthora infestans; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology*; D035281:Plant Tubers / Q000235:genetics / Q000469:parasitology*; D011198:Solanum tuberosum / Q000235:genetics / Q000469:parasitology*; D059467:Transcriptome