title: Simultaneous assessment of endogenous thiol compounds by LC-MS/MS.
abstract: Biological thiol compounds are very important molecules that participate in various physiological events. Alteration in levels of endogenous thiols has been suggested as a biomarker of early stage of pathological changes. We reported a chemical derivatization- and LC-MS/MS-based approach to simultaneously determine thiol compounds including glutathione (GSH), cysteine (Cys), N-acetyl cysteine (NAC), homocysteine (Hcy), and cysteinylglycine (CysGly) in biological samples. Thiol-containing samples were derivatized with monobromobimane (mBrB) at room temperature, followed by LC-MS/MS analysis. Assessment of the analytes with baseline separation was completed within 10min, using a gradient elution on a C18 reversed-phase column. Excellent linearity was observed for all analytes over their concentration ranges of 1-400μM. The lowest limits of detection (S/N=3) in a range from 0.31fmol (for NAC) to 4.98fmol (for CysGly) were achieved. The results indicate that this approach was sensitive, selective, and well suited for high-throughput quantitative determination of the biological thiols.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Sun
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Yao
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, PR China.
authors.forename: Xiucai
authors.initials: X
authors.lastname: Guo
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, PR China. Electronic address: yingpeng1999@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, PR China; Center for Developmental Therapeutics, Seattle Children's Research Institute, Division of Gastroenterology and Hepatology, Department of Pediatrics, University of Washington School of Medicine, Seattle, WA 98101, United States. Electronic address: zhengneu@yahoo.com.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000111:Acetylcysteine / Q000032:analysis* / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003545:Cysteine / Q000032:analysis* / Q000097:blood; D004151:Dipeptides / Q000032:analysis* / Q000097:blood; D005978:Glutathione / Q000032:analysis* / Q000097:blood; D006710:Homocysteine / Q000032:analysis* / Q000097:blood; D057230:Limit of Detection; D008099:Liver / Q000737:chemistry*; D008297:Male; D051379:Mice; D015203:Reproducibility of Results; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D004151:Dipeptides; D013438:Sulfhydryl Compounds; D006710:Homocysteine; C028505:cysteinylglycine; D005978:Glutathione; D003545:Cysteine; D000111:Acetylcysteine
keywords: LC–MS/MS; Simultaneous assessment; Thiol compounds