title: Simultaneous determination and pharmacokinetics of sixteen Angelicae dahurica coumarins in vivo by LC-ESI-MS/MS following oral delivery in rats.
abstract: BACKGROUND
  The roots of Angelica dahurica cv. Qibaizhi is frequently used in clinical practice as a traditional Chinese medicine. However, a comprehensive study of the pharmacokinetics of this medicine has not been carried out.
  
  
  METHODS
  A sensitive and specific liquid chromatographic-tandem mass (LC-MS/MS) spectrometric method was established to investigate pharmacokinetics of sixteen coumarins of Angelicae dahuricae Radix (ADR) in rat plasma, including xanthotoxol (1), oxypeucedanin hydrate (2), 5-hydroxy-8-methoxypsoralen (3), (-)-marmesin (4), byakangelicin (5), columbianetin (6), psoralen (7), xanthotoxin (8), neobyakangelicol (9), isoimpinellin (10), bergapten (11), heraclenin (12), oxypeucedanin ethanolate (13), imperatorin (14), phellopterin (15), isoimperatorin (16). Detection was performed on a triple quadrupole mass spectrometer in multiple-reaction-mode (MRM).
  
  
  RESULTS
  The method established in this assay was successfully applied to the pharmacokinetic study of the selected coumarins in rat plasma after oral administration of the extract of ADR, and the pharmacokinetic characteristics of sixteen coumarins were clearly elucidated.
  
  
  CONCLUSIONS
  This pharmacokinetic identification of multiple coumarins of ADR in rats provides a significant basis for better understanding the metabolic mechanism of the herb medicine.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China; School of Life Science and Engineering, Lanzhou University of Technology, 730050, Lanzhou, China.
authors.forename: Ai-Hong
authors.initials: AH
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China; Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, United States.
authors.forename: You-Bo
authors.initials: YB
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China. Electronic address: xwyang@bjmu.edu.cn.
authors.forename: Xiu-Wei
authors.initials: XW
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D029969:Angelica / Q000737:chemistry*; D000818:Animals; D002681:China; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003374:Coumarins / Q000378:metabolism* / Q000493:pharmacokinetics*; D004365:Drugs, Chinese Herbal / Q000378:metabolism / Q000493:pharmacokinetics*; D008297:Male; D010936:Plant Extracts / Q000378:metabolism / Q000493:pharmacokinetics*; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts
keywords: Angelicae Dahuricae Radix; Coumarins; LC–ESI-MS/MS; Pharmacokinetics; Traditional Chinese Medicine