title: Enhanced osteogenesis on biofunctionalized poly(ɛ-caprolactone)/poly(m-anthranilic acid) nanofibers.
abstract: Biofunctionalized nanofibers with a desired biological function can be used as a tissue engineering scaffold due to their small fiber diameters and porous structure. In the present study, poly(ɛ-caprolactone)/poly(m-anthranilic acid) nanofibers were biofunctionalized with covalent immobilization of bone morphogenetic protein-2 (BMP-2) through 1-ethyl-3-(dimethyl-aminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide activation. Fourier transform infrared analysis of the nanofiber surfaces confirmed the successful immobilization. The amount of immobilized BMP-2 was determined with bicinchoninic acid protein assay. The nanofibers before and after BMP-2 immobilization were non-cytotoxic and enhanced the attachment and proliferation of Saos-2 cells. Biofunctionalization of nanofibers with BMP-2 promoted in vitro osteogenic activity. The alkaline phosphatase activity and calcium mineralizatio of cells after 14 days of in vitro culture were enhanced on nanofibers with immobilized BMP-2.
authors.affiliation: Nanoscience and Nanoengineering, Istanbul Technical University, Istanbul, Turkey I3N/Cenimat and Physics Department, Faculty of Science and Technology, Nova University of Lisbon, Caparica, Portugal.
authors.forename: Zeliha
authors.initials: Z
authors.lastname: Guler
authors.affiliation: I3N/Cenimat and Physics Department, Faculty of Science and Technology, Nova University of Lisbon, Caparica, Portugal.
authors.forename: Jorge C
authors.initials: JC
authors.lastname: Silva
authors.affiliation: Nanoscience and Nanoengineering, Istanbul Technical University, Istanbul, Turkey Department of Chemistry, Istanbul Technical University, Istanbul, Turkey Department of Polymer Science and Technology, Istanbul Technical University, Istanbul, Turkey sarac@itu.edu.tr.
authors.forename: Abdulkadir S
authors.initials: AS
authors.lastname: Sarac
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage* / Q000737:chemistry; D002460:Cell Line; D002478:Cells, Cultured; D004058:Diffusion; D004343:Drug Implants / Q000008:administration & dosage / Q000138:chemical synthesis; D004867:Equipment Design; D006801:Humans; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds; D062367:ortho-Aminobenzoates / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D004343:Drug Implants; D011091:Polyesters; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; C016240:polycaprolactone
keywords: Poly (m-antranilic acid); biofunctionalization; bone morphogenetic protein-2; electrospun nanofiber; in vitro cell studies