title: Platelet Immobilization on Supported Phospholipid Bilayers for Single Platelet Studies.
abstract: The worldwide cardiovascular disease (CVD) epidemic is of grave concern. A major role in the etiology of CVDs is played by the platelets (thrombocytes). Platelets are anuclear cell fragments circulating in the blood. Their primary function is to catalyze clot formation, limiting traumatic blood loss in the case of injury. The same process leads to thrombosis in the case of CVDs, which are commonly managed with antiplatelet therapy. Platelets also have other, nonhemostatic functions in wound healing, inflammation, and tissue regeneration. They play a role in the early stages of atherosclerosis and the spread of cancer through metastases. Much remains to be learned about the regulation of these diverse platelet functions under physiological and pathological conditions. Breakthroughs in this regard are expected to come from single platelet studies and systems approaches. The immobilization of platelets at surfaces is advantageous for developing such approaches, but platelets are activated when they come in contact with foreign surfaces. In this work, we develop and validate a protocol for immobilizing platelets on supported lipid bilayers without activation due to immobilization. Our protocol can therefore be used for studying platelets with a wide variety of surface-sensitive techniques.
authors.affiliation: Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT) , Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Uhl
authors.affiliation: Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT) , Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Alessia
authors.initials: A
authors.lastname: Donati
authors.affiliation: Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT) , Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.|Department of Bioengineering, University of Washington , Seattle, Washington 98105, United States.
authors.forename: Ilya
authors.initials: I
authors.lastname: Reviakine
mesh_terms: D019921:Biotinylation; D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology*; D018914:Cells, Immobilized / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008051:Lipid Bilayers / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D019007:P-Selectin / Q000097:blood; D010743:Phospholipids / Q000737:chemistry; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D059010:Single-Cell Analysis / Q000379:methods; D019809:Streptavidin; D013499:Surface Properties; D049490:Systems Biology
chemical_list: D008051:Lipid Bilayers; D019007:P-Selectin; D010743:Phospholipids; C546937:SELP protein, human; D019809:Streptavidin