title: Comparing Montanide ISA 720 and 50-V2 adjuvants formulated with LmSTI1 protein of Leishmania major indicated the potential cytokine patterns for induction of protective immune responses in BALB/c mice.
abstract: Adjuvants have a key role in subunit vaccine formulations to generate protective immune responses. Herein, we present results of a comparative study on mice immunized with E. coli-derived rLmSTI1 antigen formulated with Montanide ISA 720 (Ag-M720) and ISA 50-V2 (Ag-M50) adjuvants against Leishmania major (L. major). Groups of BALB/c mice were immunized with either Ag-M720 or Ag-M50 by 3 subcutaneous injections with 3-week intervals. Three weeks after the last injection mice were challenged by L. major promastigotes. Immune responses were evaluated before, 3 weeks, and 8 weeks after challenge. Results indicated lower parasite and lesion size in vaccinated mice (the lowest for Ag-M720 indicating the best protection) which correlated with higher IFN-γ induction in immunized groups (Ag-M720 and Ag-M50) compared to control (PBS/adjuvant alone) group. Immune assays showed comparable IFN-γ, total IgG, IgG1 and IgG2a levels for Ag-M720 and Ag-M50 immunized mice but higher induction of IL-4, IL-10 and IL-17 in Ag-M50 and the highest IL-10/IL-17 ratio in Ag-M720 group followed by Ag-M50 and control groups. Altogether, results indicated that lower induction of IL-4, IL-10 and IL-17 cytokines (and/or higher ratio of IL-10/IL-17) despite comparable IFN-γ might be the reason for the superior protection in Ag-M720 group.
authors.affiliation: Immunology Department, Pasteur Institute of Iran, 69 Pasteur Ave., Tehran 13169-43551, Iran; Virology Department, Pasteur Institute of Iran, 69 Pasteur Ave., Tehran 13169-43551, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Shokri
authors.affiliation: Virology Department, Pasteur Institute of Iran, 69 Pasteur Ave., Tehran 13169-43551, Iran. Electronic address: rfarzin@pasteur.ac.ir.
authors.forename: Farzin
authors.initials: F
authors.lastname: Roohvand
authors.affiliation: Immunology Department, Pasteur Institute of Iran, 69 Pasteur Ave., Tehran 13169-43551, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Alimohammadian
authors.affiliation: Biochemistry Department, Pasteur Institute of Iran, 69 Pasteur Ave., Tehran 13169-43551, Iran.
authors.forename: Mina
authors.initials: M
authors.lastname: Ebrahimirad
authors.affiliation: Immunology Department, Pasteur Institute of Iran, 69 Pasteur Ave., Tehran 13169-43551, Iran. Electronic address: sohary@yahoo.com.
authors.forename: Soheila
authors.initials: S
authors.lastname: Ajdary
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006360:Heat-Shock Proteins / Q000276:immunology*; D018320:Leishmania major; D054332:Leishmaniasis Vaccines / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology*; D008353:Mannitol / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009829:Oleic Acids / Q000494:pharmacology; D015800:Protozoan Proteins / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D006360:Heat-Shock Proteins; D054332:Leishmaniasis Vaccines; D009829:Oleic Acids; D015800:Protozoan Proteins; C109320:stress-inducible protein 1, Leishmania major; C001681:mannide monooleate; D008353:Mannitol
keywords: Leishmania major; LmSTI1; Montanide