title: Emergence of ciprofloxacin-resistant extended-spectrum β-lactamase-producing enteric bacteria in hospital wastewater and clinical sources.
abstract: This study aimed to evaluate the incidence of ciprofloxacin-resistant extended-spectrum β-lactamase (ESBL)-producing enteric bacteria in hospital wastewater and clinical sources. Enteric bacteria, mainly Escherichia coli, were isolated from clinical sources (urinary tract and gastrointestinal tract infections; 80 isolates) and hospital wastewater (103 isolates). The antibiotic resistance profile and ESBL production of the isolates were investigated by disc diffusion assay and combined disc diffusion test, respectively. Plasmid profiling was performed by agarose gel electrophoresis, and elimination of resistance markers was performed by a plasmid curing experiment. Antibiotic susceptibility testing revealed a high incidence of β-lactam resistance, being highest to ampicillin (88.0%) followed by amoxicillin, ceftriaxone, cefpodoxime, cefotaxime, aztreonam, cefepime and ceftazidime. Among the non-β-lactam antibiotics, the highest resistance was recorded to nalidixic acid (85.7%). Moreover, 50.8% of enteric bacteria showed resistance to ciprofloxacin. Among 183 total enteric bacteria, 150 (82.0%) exhibited multidrug resistance. ESBL production was detected in 78 isolates (42.6%). A significantly higher incidence of ciprofloxacin resistance was observed among ESBL-producing enteric bacteria both in clinical (P=0.0015) and environmental isolates (P=0.012), clearly demonstrating a close association between ESBL production and ciprofloxacin resistance. Plasmid profiling of selected ESBL-positive strains indicated the presence of one or more plasmids of varying sizes. Plasmid curing resulted in loss of ciprofloxacin and cefotaxime resistance markers simultaneously from selected ESBL-positive isolates, indicating the close relationship of these markers. This study revealed a common occurrence of ciprofloxacin-resistant ESBL-producing enteric bacteria both in hospital wastewater and clinical sources, indicating a potential public health threat.
authors.affiliation: Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Meenu
authors.initials: M
authors.lastname: Maheshwari
authors.affiliation: Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Nawar Hadi
authors.initials: NH
authors.lastname: Yaser
authors.affiliation: Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Suraiya
authors.initials: S
authors.lastname: Naz
authors.affiliation: Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Mansha
authors.initials: M
authors.lastname: Fatima
authors.affiliation: Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh 202002, India. Electronic address: ahmadiqbal8@yahoo.co.in.
authors.forename: Iqbal
authors.initials: I
authors.lastname: Ahmad
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D000069196:Gastrointestinal Microbiome; D006761:Hospitals; D007194:India; D010957:Plasmids / Q000235:genetics; D062065:Wastewater / Q000382:microbiology*; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D062065:Wastewater; D001618:beta-Lactamases
keywords: Ciprofloxacin; Drug resistance; Enteric bacteria; Extended-spectrum β-lactamases; Hospital wastewater; β-Lactam antibiotics