title: Monitoring the primo vascular system in lymphatic vessels by using window chambers.
abstract: This study aims to develop a window chamber system in the skin of rats and to monitor the primo vascular system (PVS) inside the lymphatic vessels along the superficial epigastric vessels. The PVS in lymphatic vessels has been observed through many experiments under in vivo conditions, but monitoring the in vivo PVS in situ inside lymphatic vessels for a long time is difficult. To overcome the obstacles, we adapted the window chamber system for monitoring the PVS and Alcian blue (AB) staining dye solution for the contrast agent. The lymphatic vessels in the skin on the lateral side of the body, connecting the inguinal lymph nodes to the axillary lymph nodes, were the targets for setting the window system. After AB had been injected into the inguinal lymph nodes with a glass capillary, the morphological changes of the stained PVS were monitored through the window system for up to twenty hours, and the changes in the AB intensity in the PVS were quantified by using image processing. The results and histological images are presented in this study.
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, South Korea; Pharmacopuncture Medical Research Center, Korean Pharmacopuncture Institute, Seoul 157-801, South Korea.
authors.forename: Jungdae
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Nano-Optical Engineering, Korea Polytechnic University, Siheung 429-793, South Korea.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, South Korea; Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, Suwon 443-270, South Korea.
authors.forename: Sharon Jiyoon
authors.initials: SJ
authors.lastname: Jung
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, South Korea.
authors.forename: Hyun-Ji
authors.initials: HJ
authors.lastname: Gil
authors.affiliation: Department of Anesthesiology, College of Medicine, Korea University, Seoul 02841, South Korea.
authors.forename: Seung Zhoo
authors.initials: SZ
authors.lastname: Yoon
authors.affiliation: Department of Nano-Optical Engineering, Korea Polytechnic University, Siheung 429-793, South Korea.
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Kim
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, South Korea.
authors.forename: Kwang-Sup
authors.initials: KS
authors.lastname: Soh
keywords: (170.0110) Imaging systems; (170.0170) Medical optics and biotechnology; (170.2655) Functional monitoring and imaging; (170.3880) Medical and biological imaging; (170.4580) Optical diagnostics for medicine