title: Mitochondrial Protein Profile in Mice with Low or Excessive Selenium Diets.
abstract: Dietary selenium putatively prevents oxidative damage, whereas excessive selenium may lead to animal disorder. In this study, we investigated the effects of low and excessive levels of dietary selenium on oxidative stress and mitochondrial proteins in mouse liver. Six to eight week old mice were fed a diet with low, excessive, or moderate (control) levels of selenium (sodium selenite). The selenium concentration and oxidative stress-related parameters in hepatic mitochondria were evaluated. Two-dimensional electrophoresis and mass spectrometry were applied to identify the differentially-expressed proteins associated with dietary selenium. The selenium content of the livers in mice with the low selenium diet was significantly lower than that of the control, while that of mice fed excessive levels was significantly higher. In both groups oxidative stress in hepatic mitochondria was found; accompanied by lower superoxide dismutase (SOD) and glutathione peroxidase (GPX) levels and higher malondialdehyde (MDA) content, compared with the control group. Furthermore, ten proteins in the hepatic mitochondria of the selenium-low or -excessive groups with more than two-fold differences in abundance compared with the control group were identified. The differentially-expressed proteins in hepatic mitochondria may be associated with dietary (low or excessive) selenium-induced oxidative stress.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. hulianmei@scau.edu.cn.|Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou 510642, China. hulianmei@scau.edu.cn.
authors.forename: Lianmei
authors.initials: L
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. wcongcong321@126.com.
authors.forename: Congcong
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. sunshine@scau.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. yanhaoin1989@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. lying@scau.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. panjq@scau.edu.cn.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Pan
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. zhxtang@163.com.
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Tang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D012643:Selenium / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry
chemical_list: D024101:Mitochondrial Proteins; D012643:Selenium
keywords: 2-dimensional electrophoresis; dietary selenium; hepatic mitochondrial proteomics; mass spectrometry; mouse