title: Identification of glycerophospholipid molecular species of mussel (Mytilus edulis) lipids by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry.
abstract: This study was carried out to identify the glycerophosphocholine (GPCho), glycerophosphoethanolamine (GPEtn) and glycerophosphoserine (GPSer) compositions in lipids extracted from mussels using Folch, Bligh-Dyer and methyl-tert-butyl ether (MTBE) methods by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS). The molecular species of GPCho, GPEtn and GPSer were characterized according to the MS and MS/MS information. A semi-quantitative method using internal standard was established to compare the difference in glycerophospholipids (GP) between samples recovered with different methods. At least 212, 230 and 206 GP species were identified, respectively, from lipids recovered by Folch, Bligh-Dyer and MTBE methods. Most of the abundant GP species in mussels contained EPA and DHA. Some GP species with low content were not present in lipids recovered by the Folch and MTBE methods when compared with that recovered by the Bligh-Dyer method. However, for most GP species in lipids recovered by different methods, no quantitative differences existed.
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Fa-Wen
authors.initials: FW
authors.lastname: Yin
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B3X9, Canada. Electronic address: zdyzf1@163.com.
authors.forename: Da-Yong
authors.initials: DY
authors.lastname: Zhou
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Zhong-Yuan
authors.initials: ZY
authors.lastname: Liu
authors.affiliation: The Innovation Centre of Food Nutrition and Human Health, China Agricultural University, Beijing 100083, PR China.
authors.forename: Xiao-Pei
authors.initials: XP
authors.lastname: Hu
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Liu
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Song
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qin
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; The Innovation Centre of Food Nutrition and Human Health, China Agricultural University, Beijing 100083, PR China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, Tianjin 300457, PR China. Electronic address: zhubeiwei@163.com.
authors.forename: Bei-Wei
authors.initials: BW
authors.lastname: Zhu
authors.affiliation: Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B3X9, Canada.
authors.forename: Fereidoon
authors.initials: F
authors.lastname: Shahidi
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020404:Glycerophospholipids / Q000378:metabolism*; D049880:Mytilus edulis / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D020404:Glycerophospholipids; D010714:Phosphatidylethanolamines; C002449:glycerophosphoethanolamine
keywords: Glycerophosphocholine; Glycerophosphoethanolamine; Glycerophosphoserine; HPLC-ESI-MS/MS; Molecular species; Mussel