title: Mediastinal Clear Cell Lymphoma-A Distinct Entity of B-Cell Derived Lymphoma as Shown by Immunotyping and Analysis of Gene Rearrangements.
abstract: Mediastinal clear cell lymphoma (MCCL) is regarded as a distinct subtype of Non-Hodgkin's-lymphoma. We have analyzed gene rearrangements of eight cases of newly diagnosed MCCL in order to verify their alignment to the B-cell lineage as suggested by immunotyping. The lymphoma cells shared a common immunophenotype which consisted of CD10-, CD19+, CD20+, and CD21-. Rearrangements of the heavy chain of the immunoglobulin gene were found in all eight cases studied. In contrast, the beta chain of the T-cell antigen receptor gene was not rearranged. No rearrangements of the kappa light chain gene were detected. There was no evidence for a t(14;18) or a t(8;14). In conclusion, MCCL seems to be a B-cell derived tumor, which is distinct from other known lymphomas both by its immunophenotype and genotype.
authors.affiliation: a Department of Internal, Medicine and Policlinic of the Free University, Berlin.
authors.forename: W U
authors.initials: WU
authors.lastname: Knauf
authors.affiliation: b Department of Internal, Medicine and Policlinic of the University, Heidelberg.
authors.forename: A D
authors.initials: AD
authors.lastname: Ho
authors.affiliation: b Department of Internal, Medicine and Policlinic of the University, Heidelberg.
authors.forename: G
authors.initials: G
authors.lastname: Heger
authors.affiliation: b Department of Internal, Medicine and Policlinic of the University, Heidelberg.
authors.forename: B
authors.initials: B
authors.lastname: Doerken
authors.affiliation: c Institute of Pathology of the University, Im Neuenheimer Feld, D-6900, Heidelberg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Moeller
keywords: mediastinal lymphoma; molecular analysis immunotyping