title: Bioengineering of differentiated hepatocytes with human factor IX-expressing plasmids in vitro.
abstract: For somatic gene therapy of hemophilia B, hepatocytes as the main cellular host for expression of hFIX are attractive targets. In gene therapy protocols, an efficient expression vector equipped with cis-regulatory elements such as introns is required. With this in mind, hFIX-expressing plasmids equipped with different combinations of 2 human β-globin (hBG) introns inside the hFIX-cDNA and Kozak element were used for bioengineering of HepG2 cells as a model for differentiated hepatocytes and CHO cells a cell line generally used to produce recombinant hFIX (rhFIX). In HepG2 cells, the highest hFIX secretion level occurred for the intron-less plasmid with 8.5 to 53.8- fold increases, while in CHO cells, the hBG intron-I containing plasmid induced highest hFIX secretion level with 2.3 to 14.3-fold increases as compared to other plasmids. The first hBG intron appears to be more effective than the second one in both cell lines. The expression level was further increased upon the inclusion of the Kozak element. The highest hFIX activity was obtained from the cells that carrying the intron-less plasmids with 470 mU/ml and 25 mU/ml for HepG2 and CHO cells respectively. Secretion of active hFIX by all constructs was documented except for hBG intron-II containing construct in both cell lines. HepG2 cells were able to secret higher hFIX levels by 0.6 to 112.2-fold increases with activity by 5.3 to 16.4-fold increases compared to CHO cells transfected with the same constructs. Presence of both hBG intron-I and II inside the hFIX-cDNA provides properly spliced hFIX transcripts in both cell lines. In conclusion, the advantages of hBG introns as attractive cis-regulatory elements to obtain higher expression level of hFIX particularly in CHO cells were demonstrated. Hepatocytes could be effectively bioengineered with the use of plasmid vectors and this strategy may provide a potential in-vitro source of functional hepatocytes for ex-vivo gene therapy of hemophilias and production of rhFIX in vitro.
authors.affiliation: a Department of Cellular and Molecular Biotechnology , Institute of Biotechnology, Urmia University , Urmia , Iran.
authors.forename: Azadeh Sadat
authors.initials: AS
authors.lastname: Azadbakhsh
authors.affiliation: a Department of Cellular and Molecular Biotechnology , Institute of Biotechnology, Urmia University , Urmia , Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Sam
authors.affiliation: b Department of Histology and Embryology , Faculty of Science, Urmia University , Urmia , Iran.
authors.forename: Farrah
authors.initials: F
authors.lastname: Farokhi
mesh_terms: D000818:Animals; D057005:Bioengineering; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005786:Gene Expression Regulation; D015316:Genetic Therapy / Q000379:methods; D002836:Hemophilia B / Q000628:therapy; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D007438:Introns; D010957:Plasmids / Q000235:genetics; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D055544:beta-Globins / Q000235:genetics / Q000378:metabolism
chemical_list: D055544:beta-Globins; D012697:Serine Endopeptidases; C513666:erythroelastase-IX, human
keywords: Bioengineering; hemophilia B; human factor IX (hFIX); human β-globin (hBG) intron; plasmid vector; recombinant human factor IX (rhFIX); somatic gene therapy