title: A detailed proteomic profiling of plasma membrane from zebrafish brain.
abstract: Zebrafish (Danio rerio) is a well-established model organism in developmental biology and disease modeling. In recent years, an increasing amount of studies used zebrafish to analyze the genetic changes underlying various neurological disorders. The brain plasma membrane proteome represents the major subsets of signaling proteins and promising drug targets, but is often understudied due to traditional experimental difficulties including problems with solubility, detergent removal, or low abundance. Here, we report a comprehensive dataset of the proteins identified in the enriched plasma membrane of the zebrafish brain by applying sequential trypsin/chymotrypsin digestion with multidimensional LC-MS/MS. A total number of 97 017 peptide groups corresponding to 9201 proteins were identified. These were annotated in various molecular functions or neurological disorders. The dataset of the current study provides a useful data source for further utilizing zebrafish in basic and clinical neuroscience.
authors.affiliation: Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
authors.forename: Roman
authors.initials: R
authors.lastname: Smidak
authors.affiliation: Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
authors.forename: Jana
authors.initials: J
authors.lastname: Aradska
authors.affiliation: Children's Cancer Research Institute, St. Anna Kinderkrebsforschung, Innovative Cancer Models, Vienna, Austria.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Kirchberger
authors.affiliation: Children's Cancer Research Institute, St. Anna Kinderkrebsforschung, Innovative Cancer Models, Vienna, Austria.
authors.forename: Martin
authors.initials: M
authors.lastname: Distel
authors.affiliation: Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
authors.forename: Fernando J
authors.initials: FJ
authors.lastname: Sialana
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Life Sciences, University of Vienna, Vienna, Austria.
authors.forename: Judith
authors.initials: J
authors.lastname: Wackerlig
authors.affiliation: Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Diana
authors.initials: D
authors.lastname: Mechtcheriakova
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Life Sciences, University of Vienna, Vienna, Austria.
authors.forename: Gert
authors.initials: G
authors.lastname: Lubec
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D063990:Gene Ontology; D040901:Proteomics; D015027:Zebrafish / Q000378:metabolism*
keywords: Brain; Membrane proteome; Zebrafish