title: Direct Quantification of DNA Base Composition by Surface-Enhanced Raman Scattering Spectroscopy.
abstract: Design of ultrasensitive DNA sensors based on the unique physical properties of plasmonic nanostructures has become one of the most exciting areas in nanomedicine. However, despite the vast number of proposed applications, the determination of the base composition in nucleic acids, a fundamental parameter in genomic analyses and taxonomic classification, is still restricted to time-consuming and poorly sensitive conventional methods. Herein, we demonstrate the possibility of determining the base composition in single- and double-stranded DNA by using a simple, low-cost, high-throughput, and label-free surface-enhanced Raman scattering (SERS) method in combination with cationic nanoparticles.
authors.affiliation: Medcom Advance , Viladecans Business Park, Edificio Brasil, Bertran i Musitu 83-85, 08840 Viladecans, Barcelona, Spain.|Centro Tecnológico de la Química de Catalunya and Universitat Rovira I Virgili , Carrer de Marcel•lí Domingo s/n, 43007 Tarragona, Spain.
authors.forename: Judit
authors.initials: J
authors.lastname: Morla-Folch
authors.affiliation: Medcom Advance , Viladecans Business Park, Edificio Brasil, Bertran i Musitu 83-85, 08840 Viladecans, Barcelona, Spain.|Centro Tecnológico de la Química de Catalunya and Universitat Rovira I Virgili , Carrer de Marcel•lí Domingo s/n, 43007 Tarragona, Spain.|ICREA , Passeig Lluís Companys 23, 08010 Barcelona, Spain.
authors.forename: Ramon A
authors.initials: RA
authors.lastname: Alvarez-Puebla
authors.affiliation: Medcom Advance , Viladecans Business Park, Edificio Brasil, Bertran i Musitu 83-85, 08840 Viladecans, Barcelona, Spain.|Centro Tecnológico de la Química de Catalunya and Universitat Rovira I Virgili , Carrer de Marcel•lí Domingo s/n, 43007 Tarragona, Spain.
authors.forename: Luca
authors.initials: L
authors.lastname: Guerrini
mesh_terms: D004247:DNA / Q000737:chemistry*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D004247:DNA