title: Acid-Sensitive Sheddable PEGylated PLGA Nanoparticles Increase the Delivery of TNF-α siRNA in Chronic Inflammation Sites.
abstract: There has been growing interest in utilizing small interfering RNA (siRNA) specific to pro-inflammatory cytokines, such as tumor necrosis factor-α ( TNF-α), in chronic inflammation therapy. However, delivery systems that can increase the distribution of the siRNA in chronic inflammation sites after intravenous administration are needed. Herein we report that innovative functionalization of the surface of siRNA-incorporated poly (lactic-co-glycolic) acid (PLGA) nanoparticles significantly increases the delivery of the siRNA in the chronic inflammation sites in a mouse model. The TNF-α siRNA incorporated PLGA nanoparticles were prepared by the standard double emulsion method, but using stearoyl-hydrazone-polyethylene glycol 2000, a unique acid-sensitive surface active agent, as the emulsifying agent, which renders (i) the nanoparticles PEGylated and (ii) the PEGylation sheddable in low pH environment such as that in chronic inflammation sites. In a mouse model of lipopolysaccharide-induced chronic inflammation, the acid-sensitive sheddable PEGylated PLGA nanoparticles showed significantly higher accumulation or distribution in chronic inflammation sites than PLGA nanoparticles prepared with an acid-insensitive emulsifying agent (i.e., stearoyl-amide-polyethylene glycol 2000) and significantly increased the distribution of the TNF-α siRNA incorporated into the nanoparticles in inflamed mouse foot.
authors.affiliation: Pharmaceutics Division, College of Pharmacy, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Abdulaziz M
authors.initials: AM
authors.lastname: Aldayel
authors.affiliation: Pharmaceutics Division, College of Pharmacy, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Youssef W
authors.initials: YW
authors.lastname: Naguib
authors.affiliation: Pharmaceutics Division, College of Pharmacy, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Hannah L
authors.initials: HL
authors.lastname: O'Mary
authors.affiliation: Pharmaceutics Division, College of Pharmacy, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: Pharmaceutics Division, College of Pharmacy, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Niu
authors.affiliation: Pharmaceutics Division, College of Pharmacy, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Tinashe B
authors.initials: TB
authors.lastname: Ruwona
authors.affiliation: Pharmaceutics Division, College of Pharmacy, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Cui