title: Inflammation-related pro-apoptotic activity of exopolysaccharides isolated from Lactococcus lactis subsp. lactis.
abstract: Exopolysaccharides (EPS) have attracted attention recently for possible use in suppressing early stage breast cancer. In this study, a mannan EPS produced by Lactococcus lactis subsp. lactis was found to affect the production of inflammatory cytokines. EPS (300 μg/ml) can significantly enhance tumour necrosis factor alpha and inducible NO synthase release in MCF-7 cells compared to control cells in a concentration-dependent manner. Also the intracellular calcium level was found to increase with the concentration of EPS. After EPS-treatment, a significant reduction in mitochondrial potential was observed, as was nuclear condensation and cell shrinkage. These results may be helpful in further understanding the anti-tumour properties of lactic acid bacteria.
authors.affiliation: 1 Department of Food Science and Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, Jiangsu, China P.R.|3 Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China P.R.
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
authors.affiliation: 2 Department of Landscape Engineering, Heze University, Heze 274051, Shandong, China P.R.
authors.forename: G
authors.initials: G
authors.lastname: Wang
authors.affiliation: 1 Department of Food Science and Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, Jiangsu, China P.R.|3 Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China P.R.
authors.forename: D
authors.initials: D
authors.lastname: Pan
authors.affiliation: 1 Department of Food Science and Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, Jiangsu, China P.R.
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.affiliation: 3 Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China P.R.
authors.forename: X
authors.initials: X
authors.lastname: Zeng
authors.affiliation: 3 Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China P.R.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.affiliation: 3 Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China P.R.
authors.forename: J
authors.initials: J
authors.lastname: Cao
mesh_terms: D001943:Breast Neoplasms / Q000517:prevention & control*; D002118:Calcium / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D007249:Inflammation; D013294:Lactococcus lactis / Q000737:chemistry*; D061986:MCF-7 Cells; D008351:Mannans / Q000302:isolation & purification / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D008351:Mannans; D011135:Polysaccharides, Bacterial; D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II; D002118:Calcium
keywords: MCF-7 cells; anti-tumour effects; apoptosis; caspases; exopolysaccharides