title: Association between the vitamin D receptor gene polymorphism and osteoporosis.
abstract: The influence of the vitamin D receptor (VDR) gene for the risk of osteoporosis remains to be elucidated. The aim of the present study was to understand the distribution of various single-nucleotide polymorphisms (SNPs) within theVDRgene and its association with the risk of osteoporosis. In total, 378 subjects without a genetic relationship were recruited to the study between January 2013 and July 2015. The subjects were divided into three groups, which were the normal (n=234), osteoporosis (n=65) and osteoporosis with osteoporotic fracture (n=79) groups. Three pertinent SNPs of theVDRgene rs17879735 (ApaI, Allele A/a, SNP C>A) were examined with polymerase chain reaction-restriction fragment length polymorphism. The bone mineral density (BMD) of the lumbar spine (L2-L4), femoral neck, Ward's and Tro was measured using dual-energy X-ray absorptiometry. The distributions of genotype frequencies aa, AA and Aa were 48.68, 42.86 and 8.46%, separately. Following analysis of each site, BMD, body mass index (BMI) and age, BMD for each site was negatively correlated with age (P<0.01) and positively correlated with BMI (P<0.01). Correction analysis revealed that there were significant differences in the Ward's triangle BMD among each genotype (P<0.05), in which the aa genotype exhibited the lower BMD (P<0.05). No significant difference was identified among the different genotypes in the occurrence of osteoporosis with osteoporotic fracture (P>0.05). In conclusion, these indicated that theVDRgeneApaI polymorphisms had an important role in the osteoporosis risk.
authors.affiliation: Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Ju
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: De-Peng
authors.initials: DP
authors.lastname: Shang
authors.affiliation: Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Fu
authors.affiliation: Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Hao-Yi
authors.initials: HY
authors.lastname: Ling
authors.affiliation: Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Hou
authors.affiliation: Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Lu
keywords: bone mineral density; osteoporosis; polymorphism; vitamin D receptor