title: Effect of FUT3 gene silencing with miRNA on proliferation, invasion and migration abilities of human KATO-III gastric cancer cell line.
abstract: This study investigated the effects of FUT3 gene expression inhibition with miRNA on the proliferation, invasion and migration abilities of KATO-III cells. KATO-III cells were transfected with plasmid pcDNA™6.2-GW/EmGFP-FUT3-miR(FUT3-miRNA) and negative control plasmid in mediation of liposome, respectively, using untransfected cells as blank controls. Forty-eight hours after transfection, FUT3 mRNA levels were tested by RT-PCR. Levels of sLeA proteins were assayed by Western blot. The effects of FUT3-miRNA on the proliferation, invasion and migration of KATO-III cells were determined by CCK8 testing and Transwell assays, respectively. Results indicate that the transfection of FUT3-miRNA may down-regulate sLeA protein expression on the surface of KATO-III cells, and significantly inhibit cell proliferation (p&lt;0.05). As compared to the negative and blank control groups, the number of invasion and migration cells in the FUT3-miRNA group decreased significantly (each p&lt;0.05). Experimental results indicate that the miRNA expression vector which targets the FUT3 gene can effectively inhibit the proliferation, migration and invasion abilities of KATO-III cells.
authors.affiliation: Fourth People's Hospital of Jinan, Taishan Medical College Department of Blood Transfusion Jinan China.
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Cai
authors.affiliation: Fourth People's Hospital of Jinan, Taishan Medical College Clinical Laboratory Jinan China.
authors.forename: X-F
authors.initials: XF
authors.lastname: Zheng
authors.affiliation: Fourth People's Hospital of Jinan, Taishan Medical College Nursing Department Jinan China.
authors.forename: C-H
authors.initials: CH
authors.lastname: Lu
authors.affiliation: Jiaozhou Central Hospital of Qingdao Clinical Laboratory Jiaozhou China.
authors.forename: Q
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: China National Heavy Duty Truck Group Corporation Hospital Jinan China.
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Fourth People's Hospital of Jinan, Taishan Medical College Department of Blood Transfusion Jinan China xyhlq@163.com.
authors.forename: Y-H
authors.initials: YH
authors.lastname: Xin
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005434:Flow Cytometry; D005647:Fucosyltransferases / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D014162:Transfection
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D005647:Fucosyltransferases; C019818:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase