title: Effects of dissolved oxygen on microbial community of single-stage autotrophic nitrogen removal system treating simulating mature landfill leachate.
abstract: The performance of four identical sequencing biofilm batch reactors (SBBR) for autotrophic nitrogen removal was investigated with 2000mg/L ammonia-containing mature landfill leachate at 30°C. The main objective of this study was to evaluate the effects of dissolved oxygen (DO) on the performance and microbial community of single-stage nitrogen removal using anammox and partial nitritation (SNAP) system. At an applied load of 0.5kgNm(-3)d(-1), average total nitrogen removal efficiency (TNRE) above 90% was long-term achieved with an optimal DO concentration of 2.7mg/L. The microelectrode-measured profiles showed the microenvironments inside the biofilms. 16S ribosomal Ribonucleic Acid (rRNA) amplicon pyrosequencing and denaturing gradient gel electrophoresis (DGGE) were used to analyze the microbial variations of different DO concentrations and different positions inside one reactor.
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wen
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China; Key Laboratory of the Three Gorges Reservoir's Eco-Environments, Ministry of Education, Chongqing University, Chongqing 400045, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China.
authors.forename: Jiale
authors.initials: J
authors.lastname: Wang
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China; Key Laboratory of the Three Gorges Reservoir's Eco-Environments, Ministry of Education, Chongqing University, Chongqing 400045, PR China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Qing
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China; Key Laboratory of the Three Gorges Reservoir's Eco-Environments, Ministry of Education, Chongqing University, Chongqing 400045, PR China. Electronic address: cquqianghe@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
mesh_terms: D052818:Autotrophic Processes; D018441:Biofilms / Q000254:growth & development; D019149:Bioreactors / Q000382:microbiology*; D058645:Denaturing Gradient Gel Electrophoresis; D058440:Denitrification; D014874:Water Pollutants, Chemical / Q000737:chemistry*; D018508:Water Purification / Q000379:methods*
chemical_list: D014874:Water Pollutants, Chemical
keywords: Autotrophic nitrogen removal; Bacterial community; Dissolved oxygen; Microenvironment