title: Preventive and curative effect of Pistacia lentiscus oil in experimental colitis.
abstract: OBJECTIVE
  to investigate the anti-inflammatory effect of the Pistacia lentiscus oil in experimental colitis model.
  
  
  METHODS
  Colitis was induced in male rats by instillation of 2,4,6-trinitrobenzenesulfonic acid (TNBS) in all groups. The experimental groups consisted of: 5 rats received Lentisc oil 2months before colitis induction (preventive group), 5 rats received the oil on the day of colitis induction (curative group) and 5 control rats. Lentisc oil was extracted from the ripe fruit of the plant by the cold press method and was analyzed by spectro-chromatography. Lentisc oil has been inserted with a standard diet at the dose of 30mg oil/100g of food/rat.
  
  
  RESULTS
  The lentisc oil sample is composed mainly by Oleic acid (47.96%), Palmitic acid (27.94%) and Linoleic acid (20.22%).There was a significant difference between control rats and treated rats with lentisc oil concerned body mass (p=0.009), bleeding index (p=0.005 and p=0.018) and diarrhea (p=0.012). Histological examination revealed a clear difference between the control and preventive groups with disappearance of erosion, decreased of cryptitis, irregular crypts and crypt loss in the preventive group. Curative group showed a significant decrease of ulceration, hyperplasia, cryptitis, irregular crypts and crypt loss compared to the control group. There was an attenuation of inflammation in the preventive group compared to the curative group without statistically significant.
  
  
  CONCLUSIONS
  Lentisc oil administration could provide a protective effect on intestinal inflammation in colitis rats induced by TNBS mainly when it is administered at a young age in preventive mode. This beneficial effect would involve a modification of arachidonic acid metabolism.
authors.affiliation: Université de Tunis El Manar, Faculté de Médecine de Tunis, Laboratoire de physiologie, 1007, Tunis, Tunisia.
authors.forename: Mustafa S
authors.initials: MS
authors.lastname: Naouar
authors.affiliation: Université de Tunis El Manar, Faculté de Médecine de Tunis, Laboratoire de physiologie, 1007, Tunis, Tunisia; Hôpital La Rabta, Service de gastroenterology, 1007, Tunis, Tunisia. Electronic address: w.mekki@laposte.net.
authors.forename: Lilia Zouiten
authors.initials: LZ
authors.lastname: Mekki
authors.affiliation: Institut National de Cancérologie Salah Azaiez de Tunis, Servie d'Anatomie Pathologique, 1006 Tunis, Tunisia.
authors.forename: Lamia
authors.initials: L
authors.lastname: Charfi
authors.affiliation: Hôpital La Rabta, Service de gastroenterology, 1007, Tunis, Tunisia.
authors.forename: Jalel
authors.initials: J
authors.lastname: Boubaker
authors.affiliation: Hôpital La Rabta, Service de gastroenterology, 1007, Tunis, Tunisia.
authors.forename: Azza
authors.initials: A
authors.lastname: Filali
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects / Q000473:pathology; D003967:Diarrhea / Q000188:drug therapy; D006470:Hemorrhage / Q000188:drug therapy; D008297:Male; D027927:Pistacia / Q000737:chemistry*; D010938:Plant Oils / Q000494:pharmacology / Q000627:therapeutic use*; D017208:Rats, Wistar; D012007:Rectum / Q000187:drug effects / Q000473:pathology
chemical_list: D010938:Plant Oils
keywords: 2,4,6-trinitrobenzene sulfonic acid-induced colitis; Fatty acid; Inflammatory bowel disease; Lentisc oil; Wistar rats