title: Meat proteins had different effects on oligopeptide transporter PEPT1 in the small intestine of young rats.
abstract: The peptide transporter 1 (PEPT1) in the apical membrane of enterocytes is the central mechanism for regulating the absorption of di- and tripeptides. Dietary proteins may affect PEPT1 abundance and peptide absorption. The present study aimed to characterize changes in PEPT1 mRNA and PEPT1 protein levels in the duodenum and jejunum of young rats after 7-day diet intervention with casein (reference), soy, beef, pork, chicken and fish proteins and further evaluate the impact on the epithelial absorption capacity. RT-PCR and western blot analyses showed that: (1) PEPT1 protein level in duodenum was higher (p < 0.05) for soy protein group than that for casein group. However, no difference was observed in jejunal PEPT1 protein level between any two diet groups (p > 0.05). The soy protein group had lower crypt depth and higher V/C ratio in the jejunum (p < 0.05). (2) PEPT1 mRNA levels were lower (p < 0.05) in rat duodenum and jejunum in pork, chicken and fish protein groups, whose trend was contrary to the results of jejunual histological observation with lower crypt depth, greater villus height and higher V/C ratio. In conclusion, different meat proteins alter distinct PEPT1 expression level and absorption capacity as reflected by gut morphology in small intestine.
authors.affiliation: a Key Laboratory of Meat Processing and Quality Control, MOE , Nanjing Agricultural University , Nanjing , P.R. China ;|b Key Laboratory of Animal Products Processing, MOA , Nanjing Agricultural University , Nanjing , P.R. China ;|c Jiang Collaborative Innovation Center of Meat Production, Processing and Quality Control , Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Li
authors.affiliation: a Key Laboratory of Meat Processing and Quality Control, MOE , Nanjing Agricultural University , Nanjing , P.R. China ;|b Key Laboratory of Animal Products Processing, MOA , Nanjing Agricultural University , Nanjing , P.R. China ;|c Jiang Collaborative Innovation Center of Meat Production, Processing and Quality Control , Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Chunbao
authors.initials: C
authors.lastname: Li
authors.affiliation: a Key Laboratory of Meat Processing and Quality Control, MOE , Nanjing Agricultural University , Nanjing , P.R. China ;|b Key Laboratory of Animal Products Processing, MOA , Nanjing Agricultural University , Nanjing , P.R. China ;|c Jiang Collaborative Innovation Center of Meat Production, Processing and Quality Control , Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Shangxin
authors.initials: S
authors.lastname: Song
authors.affiliation: a Key Laboratory of Meat Processing and Quality Control, MOE , Nanjing Agricultural University , Nanjing , P.R. China ;|b Key Laboratory of Animal Products Processing, MOA , Nanjing Agricultural University , Nanjing , P.R. China ;|c Jiang Collaborative Innovation Center of Meat Production, Processing and Quality Control , Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhao
authors.affiliation: a Key Laboratory of Meat Processing and Quality Control, MOE , Nanjing Agricultural University , Nanjing , P.R. China ;|b Key Laboratory of Animal Products Processing, MOA , Nanjing Agricultural University , Nanjing , P.R. China ;|c Jiang Collaborative Innovation Center of Meat Production, Processing and Quality Control , Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Xinglian
authors.initials: X
authors.lastname: Xu
authors.affiliation: a Key Laboratory of Meat Processing and Quality Control, MOE , Nanjing Agricultural University , Nanjing , P.R. China ;|b Key Laboratory of Animal Products Processing, MOA , Nanjing Agricultural University , Nanjing , P.R. China ;|c Jiang Collaborative Innovation Center of Meat Production, Processing and Quality Control , Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Guanghong
authors.initials: G
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D004044:Dietary Proteins / Q000494:pharmacology*; D004063:Digestion; D015536:Down-Regulation / Q000187:drug effects; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D007421:Intestine, Small / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism*; D007583:Jejunum / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008460:Meat / Q000032:analysis*; D009842:Oligopeptides / Q000378:metabolism; D000074011:Peptide Transporter 1 / Q000235:genetics / Q000378:metabolism*; D059748:Proteolysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004044:Dietary Proteins; D009842:Oligopeptides; D000074011:Peptide Transporter 1; D012333:RNA, Messenger; C489940:Slc15a1 protein, rat
keywords: Meat proteins; intestinal villus; oligopeptide transporter PEPT1; small intestine