title: Effect of 4-week inhalation exposure to 1-bromopropane on blood pressure in rats.
abstract: The pathophysiology of hypertension is complex and multifactorial, and includes exposure to various chemical substances. Several recent studies have documented the reproductive and neurological toxicities of 1-bromopropane (1-BP). Given that 1-BP increased reactive oxygen species in the brain of rats, we hypothesized that 1-BP also has cardiovascular toxicity through increased oxidative stress. To test this hypothesis, male F344 and Wistar Nagoya rats (n = 7-8 per group per test) were exposed to 0 or 1000 ppm of 1-BP via inhalation for 4 weeks (8 h per day, 7 days per week). The exposure to 1-BP increased systolic blood pressure. This effect was associated with a significant decrease in the reduced/oxidized glutathione ratio. A significant increase in nitrotyrosine levels, activation of the NADPH oxidase pathway, which was evidenced by upregulation of gp91phox, a NADPH oxidase subunit, and significant decreases in the expressions of antioxidant molecules such as Cu/Zn- and Mn-superoxide dismutase catalase, and nuclear factor erythroid 2-related factor 2, were observed in the aortas of Wistar Nagoya rats exposed to 1-BP. Our results indicate that subacute (4-week) inhalation exposure to 1-BP increases blood pressure and suggest that this cardiovascular toxic effect is due, at least in part, to increased oxidative stress mediated through activation of the NADPH oxidase pathway. Further study is needed to assess whether NADPH oxidase activation causes the increase in blood pressure in the rats exposed to 1-BP. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Environmental and Occupational Health, Nagoya University Graduate School of Medicine, Nagoya, Japan.|School of Public Health, Anhui Medical University, Anhui, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Human Functional Genomics, Life Science Research Center, Mie University, Tsu, Japan.
authors.forename: Sahoko
authors.initials: S
authors.lastname: Ichihara
authors.affiliation: Department of Human Functional Genomics, Life Science Research Center, Mie University, Tsu, Japan.|Japanese Red Cross Wakayama Medical Center, Wakayama, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yamada
authors.affiliation: Department of Environmental and Occupational Health, Nagoya University Graduate School of Medicine, Nagoya, Japan.|Department of Obstetrics and Gynecology, Shri Sathya Sai Medical College Hospital and Research Center, Tamil Nadu, India.
authors.forename: Shameema
authors.initials: S
authors.lastname: Banu
authors.affiliation: Department of Environmental and Occupational Health, Nagoya University Graduate School of Medicine, Nagoya, Japan.|Department of Occupational and Environmental Health, Tokyo University of Science, Noda, Japan.
authors.forename: Gaku
authors.initials: G
authors.lastname: Ichihara
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D006842:Hydrocarbons, Brominated / Q000633:toxicity; D019570:Inhalation Exposure / Q000009:adverse effects* / Q000032:analysis; D008297:Male; D019255:NADPH Oxidases / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects*; D011916:Rats, Inbred F344; D017208:Rats, Wistar
chemical_list: D000393:Air Pollutants; D000975:Antioxidants; D006842:Hydrocarbons, Brominated; D019255:NADPH Oxidases; C118559:1-bromopropane
keywords: 1-bromopropane; NADPH oxidase; antioxidant molecules; blood pressure; cardiovascular system; oxidative stress