title: Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells.
abstract: Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact molecular mechanism is still unknown. This study is aimed at understanding the effects of FKB on HeLa cells using a microarray-based mRNA expression profiling and proteome profiling of stress-related proteins. The results of this study suggest that FKB induced cell death through p21-mediated cell cycle arrest and activation of p38. However, concurrent activation of antioxidant-related pathways and iron sequestration pathway followed by activation of ER-resident stress proteins clearly indicate that FKB failed to induce apoptosis in HeLa cells via oxidative stress. This effect implies that the protection of HeLa cells by FKB from H2O2-induced cell death is via neutralization of reactive oxygen species.
authors.affiliation: 1 Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Swee Keong
authors.initials: SK
authors.lastname: Yeap
authors.affiliation: 1 Universiti Putra Malaysia, Serdang, Selangor, Malaysia.|2 Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
authors.forename: Nadiah
authors.initials: N
authors.lastname: Abu
authors.affiliation: 3 Universiti Malaysia Pahang, LebuhrayaTunRazak, Kuantan, Pahang.
authors.forename: Nadeem
authors.initials: N
authors.lastname: Akthar
authors.affiliation: 4 The University of Nottingham Malaysia Campus, JalanBroga, Semenyih, Selangor, Malaysia.
authors.forename: Wan Yong
authors.initials: WY
authors.lastname: Ho
authors.affiliation: 5 Cantho University, CanTho City, Vietnam.
authors.forename: Huynh
authors.initials: H
authors.lastname: Ky
authors.affiliation: 1 Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Sheau Wei
authors.initials: SW
authors.lastname: Tan
authors.affiliation: 1 Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Noorjahan Banu
authors.initials: NB
authors.lastname: Alitheen
authors.affiliation: 6 Universiti Malaya, Kuala Lumpur, Malaysia.
authors.forename: Tunku
authors.initials: T
authors.lastname: Kamarul
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D050759:Cyclin-Dependent Kinase Inhibitor p21; D005419:Flavonoids; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C452039:flavokawain B; D006861:Hydrogen Peroxide; D048051:p38 Mitogen-Activated Protein Kinases
keywords: HeLa; antioxidant; cervical cancer; flavokawain B; iron sequestration