title: The Impact of Autologous Fat Grafting on Breast Cancer: An Experimental Model Using Magnetic Resonance Imaging.
abstract: BACKGROUND
  Although fat grafting is a common technique to repair defects after breast cancer reconstruction surgery and has a low complication rate, the relation between fat grafting and the risk of breast cancer is unknown. Clinical trials to investigate this connection can elucidate the benefits and potential risks of fat grafting in oncology patients.
  
  
  OBJECTIVE
  To establish an efficient experimental model, using magnetic resonance imaging (MRI) scans, for comparing different breast tumor study groups post-fat grafting.
  
  
  METHODS
  Breast tumor cells were injected into immunocompromised mice. After tumors formed they were removed. Liposuction was performed in a female human donor and fat was collected. Cells were extracted from the fat by enzymatic digestion. Immunocompromised mice were randomized into four groups: a preliminary experiment group and three equal groups according to the type of fat graft: (i) fresh fat enriched with adipose-derived mesenchymal stem cells (AdMSCs), (ii) fresh fat without cell enrichment, and (iii) no fat injected. Tumor volume was assessed by serial MRI scans.
  
  
  RESULTS
  The rate of tumor growth was higher in the enriched fat group compared to the non-enriched fat group.
  
  
  CONCLUSIONS
  This experimental model is an effective measurable method, allowing future investigation of the effect of autologous fat on breast cancer.
authors.forename: Eran
authors.initials: E
authors.lastname: Millet
authors.forename: Josef
authors.initials: J
authors.lastname: Haik
authors.forename: Elad
authors.initials: E
authors.lastname: Ofir
authors.forename: Yael
authors.initials: Y
authors.lastname: Mardor
authors.forename: Eyal
authors.initials: E
authors.lastname: Winkler
authors.forename: Moti
authors.initials: M
authors.lastname: Harats
authors.forename: Ariel
authors.initials: A
authors.lastname: Tessone
mesh_terms: D000818:Animals; D001940:Breast / Q000473:pathology / Q000601:surgery*; D001943:Breast Neoplasms / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D016462:Mammaplasty / Q000009:adverse effects / Q000379:methods; D051379:Mice; D008962:Models, Theoretical; D050151:Subcutaneous Fat / Q000637:transplantation*; D014182:Transplantation, Autologous / Q000009:adverse effects / Q000379:methods; D047368:Tumor Burden