title: Sputtered Gum metal thin films showing bacterial inactivation and biocompatibility.
abstract: Super-elastic Titanium based thin films Ti-23Nb-0.7Ta-2Zr-(O) (TNTZ-O) and Ti-24Nb-(N) (TN-N) (at.%) were deposited by direct current magnetron sputtering (DCMS) in different reactive atmospheres. The effects of oxygen doping (TNTZ-O) and/or nitrogen doping (TN-N) on the microstructure, mechanical properties and biocompatibility of the as-deposited coatings were investigated. Nano-indentation measurements show that, in both cases, 1sccm of reactive gas in the mixture is necessary to reach acceptable values of hardness and Young's modulus. Mechanical properties are considered in relation to the films compactness, the compressive stress and the changes in the grain size. Data on Bacterial inactivation and biocompatibility are reported in this study. The biocompatibility tests showed that O-containing samples led to higher cells proliferation. Bacterial inactivation was concomitant with the observed pH and surface potential changes under light and in the dark. The increased cell fluidity leading to bacterial lysis was followed during the bacterial inactivation time. The increasing cell wall fluidity was attributed to the damage of the bacterial outer cell which losing its capacity to regulate the ions exchange in and out of the bacteria.
authors.affiliation: ICD-LASMIS, Université de Technologie de Troyes, UMR 6281, CNRS, Antenne de Nogent, Pôle Technologique de Haute-Champagne, 52800 Nogent, France; Nogent International Center for CVD Innovation, LRC CEA-ICD-LASMIS, UTT, Antenne de Nogent, Pôle Technologique de Haute-Champagne, 52800 Nogent, France.
authors.forename: S
authors.initials: S
authors.lastname: Achache
authors.affiliation: ICD-LASMIS, Université de Technologie de Troyes, UMR 6281, CNRS, Antenne de Nogent, Pôle Technologique de Haute-Champagne, 52800 Nogent, France; Nogent International Center for CVD Innovation, LRC CEA-ICD-LASMIS, UTT, Antenne de Nogent, Pôle Technologique de Haute-Champagne, 52800 Nogent, France. Electronic address: akram.alhussein@utt.fr.
authors.forename: A
authors.initials: A
authors.lastname: Alhussein
authors.affiliation: ICD-LASMIS, Université de Technologie de Troyes, UMR 6281, CNRS, Antenne de Nogent, Pôle Technologique de Haute-Champagne, 52800 Nogent, France; Nogent International Center for CVD Innovation, LRC CEA-ICD-LASMIS, UTT, Antenne de Nogent, Pôle Technologique de Haute-Champagne, 52800 Nogent, France.
authors.forename: S
authors.initials: S
authors.lastname: Lamri
authors.affiliation: ICD-LASMIS, Université de Technologie de Troyes, UMR 6281, CNRS, Antenne de Nogent, Pôle Technologique de Haute-Champagne, 52800 Nogent, France.
authors.forename: M
authors.initials: M
authors.lastname: François
authors.affiliation: ICD-LASMIS, Université de Technologie de Troyes, UMR 6281, CNRS, Antenne de Nogent, Pôle Technologique de Haute-Champagne, 52800 Nogent, France; Nogent International Center for CVD Innovation, LRC CEA-ICD-LASMIS, UTT, Antenne de Nogent, Pôle Technologique de Haute-Champagne, 52800 Nogent, France.
authors.forename: F
authors.initials: F
authors.lastname: Sanchette
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne, EPFL-SB-ISIC-GPAO, Station 6, 1015 Lausanne, Switzerland.
authors.forename: C
authors.initials: C
authors.lastname: Pulgarin
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne, EPFL-SB-ISIC-GPAO, Station 6, 1015 Lausanne, Switzerland.
authors.forename: J
authors.initials: J
authors.lastname: Kiwi
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne, EPFL-SB-ISIC-GPAO, Station 6, 1015 Lausanne, Switzerland. Electronic address: sami.rtimi@epfl.ch.
authors.forename: S
authors.initials: S
authors.lastname: Rtimi
mesh_terms: D000900:Anti-Bacterial Agents / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D014025:Titanium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D014025:Titanium
keywords: Antibacterial films; Biocompatibility; Magnetron sputtering; Super-elastic coatings; Titanium based thin films