title: Stability and Degradation of Caffeoylquinic Acids under Different Storage Conditions Studied by High-Performance Liquid Chromatography with Photo Diode Array Detection and High-Performance Liquid Chromatography with Electrospray Ionization Collision-Induced Dissociation Tandem Mass Spectrometry.
abstract: Caffeoylquinic acids (CQAs) are main constituents in many herbal medicines with various biological and pharmacological effects. However, CQAs will degrade or isomerize when affected by temperature, pH, light, etc. In this study, high-performance liquid chromatography with photodiode array detection (HPLC-PDA) and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was utilized to study the stability and degradation of CQAs (three mono-acyl CQAs and four di-acyl CQAs) under various ordinary storage conditions (involving different temperatures, solvents, and light irradiation). The results indicated that the stability of CQAs was mainly affected by temperature and light irradiation, while solvents did not affect it in any obvious way under the conditions studied. Mono-acyl CQAs were generally much more stable than di-acyl CQAs under the same conditions. Meanwhile, the chemical structures of 30 degradation products were also characterized by HPLC-MS(n), inferring that isomerization, methylation, and hydrolysis were three major degradation pathways. The result provides a meaningful clue for the storage conditions of CQAs standard substances and samples.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. xuemeng0421@163.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Xue
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. shhang2012@sina.cn.
authors.forename: Hang
authors.initials: H
authors.lastname: Shi
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. zhangjiao1st@163.com.
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Beijing Hospital of Traditional Chinese Medicine, Beijing 100010, China. liuqingquan2003@126.com.
authors.forename: Qing-Quan
authors.initials: QQ
authors.lastname: Liu
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. timeguan@sina.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Guan
authors.affiliation: Beijing Institution of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. zhangjiayu0615@163.com.
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. maqun99@163.com.
authors.forename: Qun
authors.initials: Q
authors.lastname: Ma
mesh_terms: D002851:Chromatography, High Pressure Liquid; D008745:Methylation; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization; D013816:Thermodynamics
chemical_list: D012997:Solvents; C472707:caffeoylquinic acid; D011801:Quinic Acid
keywords: HPLC-MSn; caffeoylquinic acids (CQAs); light irradiation; solvent; stability; temperature