title: Comprehensive Proteomic Analysis of Human Erythropoiesis.
abstract: Mass spectrometry-based proteomics now enables the absolute quantification of thousands of proteins in individual cell types. We used this technology to analyze the dynamic proteome changes occurring during human erythropoiesis. We quantified the absolute expression of 6,130 proteins during erythroid differentiation from late burst-forming units-erythroid (BFU-Es) to orthochromatic erythroblasts. A modest correlation between mRNA and protein expression was observed. We identified several proteins with unexpected expression patterns in erythroid cells, highlighting a breakpoint in the erythroid differentiation process at the basophilic stage. We also quantified the distribution of proteins between reticulocytes and pyrenocytes after enucleation. These analyses identified proteins that are actively sorted either with the reticulocyte or the pyrenocyte. Our study provides the absolute quantification of protein expression during a complex cellular differentiation process in humans, and it establishes a framework for future studies of disordered erythropoiesis.
authors.affiliation: INSERM U1016, Institut Cochin, 75014 Paris, France; Centre National de la Recherche Scientifique (CNRS), UMR8104, 75014 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, 75014 Paris, France; Laboratory of Excellence GReX, 75015 Paris, France.
authors.forename: Emilie-Fleur
authors.initials: EF
authors.lastname: Gautier
authors.affiliation: INSERM U1016, Institut Cochin, 75014 Paris, France; Centre National de la Recherche Scientifique (CNRS), UMR8104, 75014 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, 75014 Paris, France; Laboratory of Excellence GReX, 75015 Paris, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Ducamp
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, 75014 Paris, France; Plateforme de Protéomique de l'Université Paris Descartes (3P5), 75014 Paris, France.
authors.forename: Marjorie
authors.initials: M
authors.lastname: Leduc
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, 75014 Paris, France; Plateforme de Protéomique de l'Université Paris Descartes (3P5), 75014 Paris, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Salnot
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, 75014 Paris, France; Plateforme de Protéomique de l'Université Paris Descartes (3P5), 75014 Paris, France.
authors.forename: François
authors.initials: F
authors.lastname: Guillonneau
authors.affiliation: Laboratory of Excellence GReX, 75015 Paris, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Dussiot
authors.affiliation: New York Blood Center, New York, NY 10065, USA.
authors.forename: John
authors.initials: J
authors.lastname: Hale
authors.affiliation: Laboratory of Excellence GReX, 75015 Paris, France; UPMC University Paris 06, UMR_S938 CDR Saint-Antoine, INSERM, Prolifération et Différenciation des Cellules Souches, 75012 Paris, France.
authors.forename: Marie-Catherine
authors.initials: MC
authors.lastname: Giarratana
authors.affiliation: INSERM U1016, Institut Cochin, 75014 Paris, France; Centre National de la Recherche Scientifique (CNRS), UMR8104, 75014 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, 75014 Paris, France; Laboratory of Excellence GReX, 75015 Paris, France.
authors.forename: Anna
authors.initials: A
authors.lastname: Raimbault
authors.affiliation: Laboratory of Excellence GReX, 75015 Paris, France; UPMC University Paris 06, UMR_S938 CDR Saint-Antoine, INSERM, Prolifération et Différenciation des Cellules Souches, 75012 Paris, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Douay
authors.affiliation: INSERM U1016, Institut Cochin, 75014 Paris, France; Centre National de la Recherche Scientifique (CNRS), UMR8104, 75014 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, 75014 Paris, France; Laboratory of Excellence GReX, 75015 Paris, France; Ligue Nationale Contre le Cancer, Equipe Labellisée, 75014 Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Lacombe
authors.affiliation: New York Blood Center, New York, NY 10065, USA.
authors.forename: Narla
authors.initials: N
authors.lastname: Mohandas
authors.affiliation: INSERM U1016, Institut Cochin, 75014 Paris, France; Centre National de la Recherche Scientifique (CNRS), UMR8104, 75014 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, 75014 Paris, France; Laboratory of Excellence GReX, 75015 Paris, France; Ligue Nationale Contre le Cancer, Equipe Labellisée, 75014 Paris, France.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Verdier
authors.affiliation: INSERM U1016, Institut Cochin, 75014 Paris, France; Centre National de la Recherche Scientifique (CNRS), UMR8104, 75014 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, 75014 Paris, France; Laboratory of Excellence GReX, 75015 Paris, France; Ligue Nationale Contre le Cancer, Equipe Labellisée, 75014 Paris, France.
authors.forename: Yael
authors.initials: Y
authors.lastname: Zermati
authors.affiliation: INSERM U1016, Institut Cochin, 75014 Paris, France; Centre National de la Recherche Scientifique (CNRS), UMR8104, 75014 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, 75014 Paris, France; Laboratory of Excellence GReX, 75015 Paris, France; Plateforme de Protéomique de l'Université Paris Descartes (3P5), 75014 Paris, France; Ligue Nationale Contre le Cancer, Equipe Labellisée, 75014 Paris, France. Electronic address: patrick.mayeux@inserm.fr.
authors.forename: Patrick
authors.initials: P
authors.lastname: Mayeux
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D004900:Erythroblasts / Q000378:metabolism / Q000502:physiology; D015672:Erythroid Precursor Cells / Q000378:metabolism / Q000502:physiology; D004920:Erythropoiesis / Q000502:physiology*; D006801:Humans; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D020543:Proteome; D012333:RNA, Messenger