title: Androgen induces gonadal soma-derived factor, Gsdf, in XX gonads correlated to sex-reversal but not Dmrt1 directly, in the teleost fish, northern medaka (Oryzias sakaizumii).
abstract: In the inbred HNI-II strain of Oryzias sakaizumii, Dmy and Gsdf are expressed in XY gonads from Stages 35 and 36, respectively, similarly to the inbred Hd-rR strain of Oryzias latipes. However, Dmrt1 respectively becomes detectable at Stage 36 and 5 days post hatching (dph) in the two strains. In XX HNI-II embryos, 17α-methyltestosterone (MT) induces Gsdf mRNA from Stage 36, accompanied by complete sex-reversal in all treated individuals (MT, 10 ng/mL), while Dmrt1 mRNA was first detectable at 5 dph. In XX d-rR, MT induced Gsdf mRNA expression and sex-reversal in only some of the treated individuals. Together, these results suggest the testis differentiation cascade in XY individuals differs between the HNI-II and Hd-rR strains. In addition, it is suggested that androgen-induced XX sex-reversal proceeds via an androgen-Gsdf-Dmrt1 cascade and that Gsdf plays an important role in sex-reversal in medaka.
authors.affiliation: Laboratory of Molecular Reproductive Biology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka 422-8526, Japan. Electronic address: horie.yoshifumi@nies.go.jp.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Horie
authors.affiliation: Laboratory of Molecular Reproductive Biology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka 422-8526, Japan; Institute of Science and Technology, Niigata University, Niigata 950-2181, Japan. Electronic address: f09m004a@alumni.niigata-u.ac.jp.
authors.forename: Taijun
authors.initials: T
authors.lastname: Myosho
authors.affiliation: Institute of Science and Technology, Niigata University, Niigata 950-2181, Japan. Electronic address: satochu@env.sc.niigata-u.ac.jp.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sato
authors.affiliation: Institute of Science and Technology, Niigata University, Niigata 950-2181, Japan. Electronic address: sakaizum@env.sc.niigata-u.ac.jp.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Sakaizumi
authors.affiliation: Institute of Science and Technology, Niigata University, Niigata 950-2181, Japan. Electronic address: shamaguc@env.sc.niigata-u.ac.jp.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hamaguchi
authors.affiliation: Laboratory of Molecular Reproductive Biology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka 422-8526, Japan. Electronic address: tohruk@u-shizuoka-ken.ac.jp.
authors.forename: Tohru
authors.initials: T
authors.lastname: Kobayashi
mesh_terms: D058531:46, XX Testicular Disorders of Sex Development / Q000378:metabolism*; D000818:Animals; D002452:Cell Count; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005854:Germ Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006066:Gonads / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008777:Methyltestosterone / Q000494:pharmacology*; D008954:Models, Biological; D009990:Oryzias / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019849:Sex Determination Processes / Q000187:drug effects / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C110547:DMRT1 protein; D029941:Fish Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D008777:Methyltestosterone
keywords: Androgen; Gsdf; Inbred HNI-II strain; Medaka; Oryzias sakaizumii; XX sex-reversal