title: Interleukin (IL)-1 in rat parturition: IL-1 receptors 1 and 2 and accessory proteins abundance in pregnant rat uterus at term - regulation by progesterone.
abstract: The role of interleukin-1 (IL-1), a pro-inflammatory cytokine, in parturition is typically noted by changes in its concentrations. Studying the expression of its receptor family, IL-1 receptor (IL-1R) 1, IL-1R2, IL-1R accessory protein (IL-1RAcP), and its predominantly brain isoform, IL-1RAcPb, during late gestation in the uterus in the Long-Evans rat is another. We assessed changes in their mRNA and protein relative abundance in the uterus and compared IL-1RAcP and IL-1RAcPb mRNA abundance in uterus, cervix, ovaries, placenta, and whole blood of Long-Evans rats during late gestation or in RU486 and progesterone-treated dams using quantitative real-time PCR and western immunoblotting. IL-1R1, IL-1RAcP, and IL-1RAcPb mRNA abundance significantly increased in the uterus at delivery whereas IL-1R2 mRNA abundance significantly decreased. IL-1R1 protein increased at term and IL-1R2 protein decreased at term compared to nonpregnant uteri. IL1-RAcPb mRNA abundance was less than IL-1RAcP, but in the lower uterine segment it was the highest of all tissues examined. RU486 stimulated preterm delivery and an increase in IL-1R1 mRNA abundance whereas progesterone administration extended pregnancy and suppressed the increase in IL-1R1. These data suggest that changes in uterine sensitivity to IL-1 occur during late gestation and suggest another level of regulation for the control of delivery. The roles for IL-1RAcP and IL-1RAcPb need to be determined, but may relate to different intracellular signaling pathways.
authors.affiliation: Departments of Obstetrics and Gynecology, Physiology & Pediatrics, University of Alberta, Edmonton, Canada Departments of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, Tokyo, Japan Departments of Obstetrics and Gynecology, Koshigaya Municipal Hospital, Koshigaya, Japan.
authors.forename: Tomohito
authors.initials: T
authors.lastname: Ishiguro
authors.affiliation: Departments of Obstetrics and Gynecology, Physiology & Pediatrics, University of Alberta, Edmonton, Canada Departments of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, Tokyo, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Takeda
authors.affiliation: Departments of Obstetrics and Gynecology, Physiology & Pediatrics, University of Alberta, Edmonton, Canada.
authors.forename: Xin
authors.initials: X
authors.lastname: Fang
authors.affiliation: Departments of Obstetrics and Gynecology, Physiology & Pediatrics, University of Alberta, Edmonton, Canada.
authors.forename: Heather
authors.initials: H
authors.lastname: Bronson
authors.affiliation: Departments of Obstetrics and Gynecology, Physiology & Pediatrics, University of Alberta, Edmonton, Canada david.olson@ualberta.ca.
authors.forename: David M
authors.initials: DM
authors.lastname: Olson
mesh_terms: D000818:Animals; D002584:Cervix Uteri / Q000187:drug effects / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D005865:Gestational Age; D006727:Hormone Antagonists / Q000494:pharmacology; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D053577:Interleukin-1 Receptor Accessory Protein / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015735:Mifepristone / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D036801:Parturition / Q000187:drug effects*; D010920:Placenta / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020318:Rats, Long-Evans; D053573:Receptors, Interleukin-1 Type I / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D053574:Receptors, Interleukin-1 Type II / Q000235:genetics / Q000378:metabolism*; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D006727:Hormone Antagonists; C000594089:IL1B protein, rat; C580803:IL1R1 protein, rat; C000610750:IL1R2 protein, rat; C507766:Il1rap protein, rat; D007375:Interleukin-1; D053577:Interleukin-1 Receptor Accessory Protein; D053583:Interleukin-1beta; D009363:Neoplasm Proteins; C104480:Ptgfrn protein, rat; D012333:RNA, Messenger; D053573:Receptors, Interleukin-1 Type I; D053574:Receptors, Interleukin-1 Type II; D015735:Mifepristone; D011374:Progesterone; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat
keywords: IL‐1 receptor; IL‐1 receptor accessory protein; IL‐1 receptor accessory protein – brain dominant; parturition; uterus