title: miR-613 inhibits proliferation and invasion of breast cancer cell via VEGFA.
abstract: MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. However, the role of microRNAs in breast cancer, has remained elusive. Here, we identified that miR-613 inhibits breast cancer cell proliferation by negatively regulates its target gene VEGFA. In breast cancer cell lines, CCK-8 proliferation assay indicated that the cell proliferation was inhibited by miR-613, while miR-613 inhibitor significantly promoted the cell proliferation. Transwell assay showed that miR-613 mimics significantly inhibited the migration and invasion of breast cancer cells, whereas miR-613 inhibitors significantly increased cell migration and invasion. Luciferase assays confirmed that miR-613 directly bound to the 3' untranslated region of VEGFA, and western blotting showed that miR-613 suppressed the expression of VEGFA at the protein levels. This study indicated that miR-613 negatively regulates VEGFA and inhibits proliferation and invasion of breast cancer cell lines. Thus, miR-613 may represent a potential therapeutic molecule for breast cancer intervention.
authors.affiliation: Breast Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Henan, China.
authors.forename: Junzhao
authors.initials: J
authors.lastname: Wu
authors.affiliation: Breast Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Henan, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yuan
authors.affiliation: Breast Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Henan, China.
authors.forename: Qixin
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Gastrointestinal Surgery Department, People's Hospital of Zhengzhou, Henan, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lu
authors.affiliation: Breast Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Henan, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Xie
authors.affiliation: Breast Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Henan, China.
authors.forename: Hanzhao
authors.initials: H
authors.lastname: Yang
authors.affiliation: Breast Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Henan, China.
authors.forename: Chengzheng
authors.initials: C
authors.lastname: Wang
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: C561374:MIRN613 microRNA, human; D035683:MicroRNAs; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Breast cancer; VEGFA; miR-613