title: Effect of pulsed electric field (PEF) on structures and antioxidant activity of soybean source peptides-SHCMN.
abstract: Recently, high-intensity pulsed electric field (PEF) has successfully used in improvement of antioxidant activity. Ser-His-Cys-Met-Asn (SHCMN) obtained from soybean protein was chosen to investigate the phenomenon of antioxidant activity improvement. Effects of PEF treatment on antioxidant activity of SHCMN were evaluated by DPPH radical inhibition. Nuclear magnetic resonance (NMR), mid-infrared (MIR), circular dichroism (CD) were used to analyze structures of SHCMN. Two-factor-at-a-time results show that DPPH radical inhibition of SHCMN is significantly (P<0.05) increased to 94.35±0.03% at conditions of electric field intensity of 5kV/cm, pulse frequency of 2400Hz, and retention time of 2h. In addition, MIR and NMR spectra show that the basic structure of peptides SHCMN is stable by PEF treatment. But the secondary structures (α-helix, β-turn, and random coil) can be affected and zeta potential of PEF-treated SHCNM was reduced to 0.59±0.03mV. The antioxidant activity improvement of SHCMN might result from the changes of secondary structures and zeta potential.
authors.affiliation: College of Food Science and Technology, Jilin University, Changchun 130062, PR China; School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Dalian 116034, PR China.
authors.forename: Songyi
authors.initials: S
authors.lastname: Lin
authors.affiliation: College of Food Science and Technology, Jilin University, Changchun 130062, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Liang
authors.affiliation: College of Food Science and Technology, Jilin University, Changchun 130062, PR China.
authors.forename: Xingfang
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Food Science and Technology, Jilin University, Changchun 130062, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xing
authors.affiliation: College of Food Science and Technology, Jilin University, Changchun 130062, PR China. Electronic address: yuanyuan_jlu@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000975:Antioxidants; D004560:Electricity; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D013025:Glycine max / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D010455:Peptides
keywords: Antioxidant activity; Antioxidant peptides SHCMN; Pulsed electric field (PEF); Secondary structures