title: Viral Immunofluorescence withRift Valley Fever VirusInfected MEFs in a 96 Well Plate.
abstract: Immunofluorescence is a method to detect viral infection in multiple types of host cells. This procedure can be adapted for both high-throughput and low-throughput assays for any virus for which there are antibodies available. Time of infection and virus multiplicity of infection (MOI) vary and should be optimized for each virus and host cell type. Here we give an example of viral immunofluorescence in a 96 well plate with aRift Valley fever virus(RVFV, strain MP12) infection in mouse embryonic fibroblasts (MEF).
authors.affiliation: Department of Microbiology, Penn Genome Frontiers Institute, The University of Pennsylvania, School of Medicine, Philadelphia, USA.
authors.forename: Theresa S
authors.initials: TS
authors.lastname: Moser
authors.affiliation: Department of Microbiology, Penn Genome Frontiers Institute, The University of Pennsylvania, School of Medicine, Philadelphia, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Cherry