title: Adsorption kinetics of low-density lipoproteins with Langmuir monolayer.
abstract: The present work utilizes the Langmuir monolayer technique to detect the adsorption kinetics of native low-density lipoproteins and their oxidized form with the lipid monolayer. We found that low-density lipoproteins and oxidized low-density lipoproteins are able to penetrate the LM up to pressure π = 9.9 and 11.6 mN/m. Also, the adsorption constants of both particles were found to depend strongly on the monolayer initial pressure. It is found that less compressed lipid monolayers could accommodate more native low-density lipoproteins than the oxidized ones due their higher binding affinity toward monolayers. The probable α-helical regions along the apoproteinB-100 secondary structure and average hydrophobicity could explain partially their adsorption kinetics into lipid monolayers. This simplified 'in vitro' study of low-density lipoprotein-monolayer interaction may serve as a step further to understand the mechanism and bioactivity of the atherosclerotic process. Also, it may shed light on the oxidized low-density lipoprotein's role in plaque formation in the innermost arterial wall in blood vessels.
authors.affiliation: Department of Physics, Hashemite University, Zarqa, Jordan. zkhattari@hu.edu.jo.
authors.forename: Ziad
authors.identifier: 0000-0003-0050-8472
authors.initials: Z
authors.lastname: Khattari
mesh_terms: D000327:Adsorption; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000737:chemistry*; D012307:Risk Factors
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein
keywords: Adsorption kinetics; Atherosclerosis; LDL oxidation; Langmuir monolayer