title: PirB Overexpression Exacerbates Neuronal Apoptosis by Inhibiting TrkB and mTOR Phosphorylation After Oxygen and Glucose Deprivation Injury.
abstract: Previous studies have proven that paired immunoglobulin-like receptor B (PirB) plays a crucial suppressant role in neurite outgrowth and neuronal plasticity after central nervous system injury. However, the role of PirB in neuronal survival after cerebral ischemic injury and its mechanisms remains unclear. In the present study, the role of PirB is investigated in the survival and apoptosis of cerebral cortical neurons in cultured primary after oxygen and glucose deprivation (OGD)-induced injury. The results have shown that rebarbative PirB exacerbates early neuron apoptosis and survival. PirB gene silencing remarkably decreases early apoptosis and promotes neuronal survival after OGD. The expression of bcl-2 markedly increased and the expression of bax significantly decreased in PirB RNAi-treated neurons, as compared with the control- and control RNAi-treated ones. Further, phosphorylated TrkB and mTOR levels are significantly downregulated in the damaged neurons. However, the PirB silencing markedly upregulates phosphorylated TrkB and mTOR levels in the neurons after the OGD. Taken together, the overexpression of PirB inhibits the neuronal survival through increased neuron apoptosis. Importantly, the inhibition of the phosphorylation of TrkB and mTOR may be one of its mechanisms.
authors.affiliation: Department of Anesthesiology, College of Stomatology, Fourth Military Medical University, Xi'an, 710032, China.|Institute of Basic and Translational Medicine & School of Basic Medical Sciences, Xi'an Medical University, Xi'an, 710021, China.
authors.forename: Zhao-Hua
authors.initials: ZH
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology, College of Stomatology, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Deng
authors.affiliation: Institute of Basic and Translational Medicine & School of Basic Medical Sciences, Xi'an Medical University, Xi'an, 710021, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Institute of Basic and Translational Medicine & School of Basic Medical Sciences, Xi'an Medical University, Xi'an, 710021, China.
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Zhang
authors.affiliation: Institute of Basic and Translational Medicine & School of Basic Medical Sciences, Xi'an Medical University, Xi'an, 710021, China.
authors.forename: Ya-Jing
authors.initials: YJ
authors.lastname: Mi
authors.affiliation: Department of Anesthesiology, College of Stomatology, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Xiang-Zhong
authors.initials: XZ
authors.lastname: Meng
authors.affiliation: Department of Anesthesiology, College of Stomatology, Fourth Military Medical University, Xi'an, 710032, China. gouxingchun@189.cn.|Institute of Basic and Translational Medicine & School of Basic Medical Sciences, Xi'an Medical University, Xi'an, 710021, China. gouxingchun@189.cn.
authors.forename: Xing-Chun
authors.initials: XC
authors.lastname: Gou
authors.affiliation: Department of Anesthesiology, College of Stomatology, Fourth Military Medical University, Xi'an, 710032, China. lixianxu2015@hotmail.com.
authors.forename: Li-Xian
authors.initials: LX
authors.lastname: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015687:Cell Hypoxia / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D020813:Receptor, trkB
chemical_list: D005947:Glucose; D008562:Membrane Glycoproteins; D018696:Neuroprotective Agents; D010100:Oxygen; D011505:Protein-Tyrosine Kinases; D011971:Receptors, Immunologic; D058570:TOR Serine-Threonine Kinases; C105949:Pirb protein, mouse; C546843:mTOR protein, mouse; C064371:Ntrk2 protein, mouse; D020813:Receptor, trkB
keywords: Apoptosis; Neuronal survival; OGD; PirB; TrkB; mTOR