title: A comparative assessment of fluoride concentration available in saliva using daily prescribed topical fluoride agents.
abstract: OBJECTIVE
  To assess the availability of fluoride concentration in saliva following the use of fluoride mouthrinse and dentifrice.
  
  
  METHODS
  The experiment was carried out in 7-15 year-old school children of Chandigarh (n = 90). The children were nonfluoride users. Baseline saliva samples were collected. The subjects were exposed to two test agents, i.e., fluoride mouthrinse (0.05%, 225 ppm F) and dentifrice (1000 ppm F) for 7 days and on the day 8, saliva samples were collected over a 20 hrs period. Wash out period of 3(1/2) months was there before the subjects were exposed to the second test agent. Fluoride in saliva was estimated using fluoride ion-specific electrode. Written informed consent was taken.
  
  
  METHODS
  Kolmogorov-Smirnov test was applied to test the normality of the variables. Mann-Whitney U-test was used to compare the fluoride concentration available in saliva at respective time intervals subsequent to use of the two test agents.
  
  
  RESULTS
  Fluoride concentration was elevated in saliva compared to baseline for both the test agents. Fluoride mouthrinse (0.05% sodium fluoride [NaF]) and dentifrice (1000 ppm monofluorophosphate [MFP]) showed a biphasic clearance. Peak in saliva occurred at 15 mins postuse. Night-time use resulted in higher concentration of fluoride in saliva compared to baseline. There was statistically significantly higher fluoride concentration available in saliva for the dentifrice at 5 hrs, 10 hrs, and 20 hrs postuse (P < 0.05).
  
  
  CONCLUSIONS
  Subsequent to the use of NaF (0.05%) daily mouthrinse and MFP dentifrice (1000 ppm) the fluoride concentration in saliva remained elevated to a level of 0.12 ppm for mouthrinse and 0.14 ppm for dentifrice compared to baseline (0.03 ppm) up to 20 hrs postuse. The therapeutic window though not yet established but suggested is 0.1-1 ppm for prevention of demineralization, indicating that daily use of fluoride mouthrinse and dentifrice provides fluoride concentration in saliva for the prevention of demineralization.
authors.affiliation: Oral Health Centre, Government Medical College and Hospital, Chandigarh, India.
authors.forename: Manjit
authors.initials: M
authors.lastname: Talwar
authors.affiliation: Oral Health Sciences Centre, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Amrit
authors.initials: A
authors.lastname: Tewari
authors.affiliation: Oral Health Sciences Centre, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: H S
authors.initials: HS
authors.lastname: Chawla
authors.affiliation: Oral Health Sciences Centre, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Vinod
authors.initials: V
authors.lastname: Sachdev
keywords: Fluoride dentifrice; fluoride mouthrinse; prevention of demineralization; saliva