title: Effect of Curcumin on Systemic T Helper 17 Cell Response; Gingival Expressions of Interleukin-17 and Retinoic Acid Receptor-Related Orphan Receptor γt; and Alveolar Bone Loss in Experimental Periodontitis.
abstract: Curcumin has anti-inflammatory and antioxidant effects and is reported to have many biologic activities. The current study examines effect of curcumin on: 1) systemic T helper 17 (Th17) cell response; 2) gingival expressions of interleukin (IL)-17 and retinoic acid receptor-related orphan receptor (ROR) γt; and 3) alveolar bone loss (ABL) in experimental periodontitis.
  
  
  
  Thirty-eight male albino Wistar rats were divided into four groups: 1) group 1 = periodontitis; 2) group 2 = periodontitis with curcumin treatment; 3) group 3 = periodontally healthy with curcumin treatment; and 4) group 4 = periodontally healthy. Curcumin was administered via oral gavage (30 mg/kg/d) for 15 days. After sacrifice via exsanguination, the following serum levels were determined using enzyme-linked immunosorbent assay: 1) IL-1β; 2) IL-6; 3) IL-17A; 4) IL-23; and 5) transforming growth factor- β. Morphometric evaluation of ABL was conducted and expression levels of IL-17 and RORγt in gingival tissues were evaluated immunohistochemically.
  
  
  
  Group 2 had significantly lower ABL than group 1 (P <0.0125). Highest expression levels of IL-17 and RORγt were observed in group 1 and were significantly higher than those in all other groups (P <0.0125). The only serum biochemical parameter significantly different among groups was level of IL-23 (P <0.05). Serum IL-23 levels were higher in groups 1 and 2 than groups 3 and 4 (P <0.0125); however, they were not significantly different for groups 1 and 2 (P >0.0125).
  
  
  
  Curcumin seems to be a promising host modulatory agent in periodontal disease pathogenesis regarding IL-17/IL-23 axis, with a decreasing effect on ABL and gingival expressions of IL-17 and RORγt.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Süleyman Demirel University, Isparta, Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Bakır
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Süleyman Demirel University, Isparta, Turkey.
authors.forename: Zuhal
authors.initials: Z
authors.lastname: Yetkin Ay
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Süleyman Demirel University.
authors.forename: H İbrahim
authors.initials: Hİ
authors.lastname: Büyükbayram
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Süleyman Demirel University.
authors.forename: Duygu
authors.initials: D
authors.lastname: Kumbul Doğuç
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Süleyman Demirel University.
authors.forename: Dilek
authors.initials: D
authors.lastname: Bayram
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Süleyman Demirel University.
authors.forename: I Aydın
authors.initials: IA
authors.lastname: Candan
authors.affiliation: Department of Public Health, Faculty of Medicine, Süleyman Demirel University.
authors.forename: Ersin
authors.initials: E
authors.lastname: Uskun
mesh_terms: D016301:Alveolar Bone Loss; D000818:Animals; D003474:Curcumin / Q000494:pharmacology*; D020381:Interleukin-17 / Q000378:metabolism*; D008297:Male; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000378:metabolism*; D010518:Periodontitis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018168:Receptors, Retinoic Acid
chemical_list: C515934:Il17a protein, rat; D020381:Interleukin-17; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D018168:Receptors, Retinoic Acid; D003474:Curcumin
keywords: Alveolar bone loss; Th17; curcumin; interleukin-17; interleukin-23; periodontitis