title: Maternal marginal iodine deficiency limits dendritic growth of cerebellar purkinje cells in rat offspring by NF-κB signaling and MAP1B.
abstract: Iodine deficiency (ID) during early pregnancy had an adverse effect on children's psychomotor and motor function. It is worth noting that maternal marginal ID tends to be a common public health problem. Whether marginal ID potentially had adverse effects on the development of cerebellum and the underlying mechanisms remain unclear. Therefore, our aim was to study the effects of marginal ID on the dendritic growth in filial cerebellar Purkinje cells (PCs) and the underlying mechanism. In the present study, we established Wistar rat models by feeding dam rats with a diet deficient in iodine and deionized water supplemented with potassium iodide. We examined the total dendritic length using immunofluorescence, and Western blot analysis was conducted to investigate the activity of nuclear factor-κB (NF-κB) signaling and microtubule-associated protein 1B (MAP1B). Our results showed that marginal ID reduced the total dendritic length of cerebellar PCs, slightly down-regulated the activity of NF-κB signaling and decreased MAP1B in cerebellar PCs on postnatal day (PN) 7, PN14, and PN21. Our study may support the hypothesis that decreased T4induced by marginal ID limits PCs dendritic growth, which may involve in the disturbance of NF-κB signaling and MAP1B on the cerebellum. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1241-1251, 2017.
authors.affiliation: Department of Occupational and Environmental Health School of Public Health, China Medical University, Shenyang, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Occupational and Environmental Health School of Public Health, China Medical University, Shenyang, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Occupational and Environmental Health School of Public Health, China Medical University, Shenyang, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Occupational and Environmental Health School of Public Health, China Medical University, Shenyang, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Occupational and Environmental Health School of Public Health, China Medical University, Shenyang, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Min
authors.affiliation: Department of Endocrinology and Metabolism, Liaoning Provincial Key Laboratory of Endocrine Diseases, the First Hospital of China Medical University, Shenyang, People's Republic of China.
authors.forename: Zhongyan
authors.initials: Z
authors.lastname: Shan
authors.affiliation: Department of Endocrinology and Metabolism, Liaoning Provincial Key Laboratory of Endocrine Diseases, the First Hospital of China Medical University, Shenyang, People's Republic of China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Teng
authors.affiliation: Department of Occupational and Environmental Health School of Public Health, China Medical University, Shenyang, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000378:metabolism*; D004032:Diet; D015536:Down-Regulation; D005260:Female; D007037:Hypothyroidism / Q000378:metabolism / Q000473:pathology; D051550:I-kappa B Kinase / Q000378:metabolism; D007455:Iodine / Q000172:deficiency*; D039382:Maternal Nutritional Physiological Phenomena; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D011247:Pregnancy; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013974:Thyroxine / Q000378:metabolism; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D008869:Microtubule-Associated Proteins; D016328:NF-kappa B; D051996:Transcription Factor RelA; C085284:microtubule-associated protein 1B; D007455:Iodine; D051550:I-kappa B Kinase; D013974:Thyroxine
keywords: MAP1B; NF-κB signaling; cerebellum; dendrite; marginal iodine deficiency; purkinje cells