title: Evaluation of ELAM-1 expression alteration in endometriosis.
abstract: BACKGROUND
  Endometriosis is a commonly-encountered gynecological disease characterized by the presence of endometrial glandular and stromal cells outside of the uterus. Several studies have been conducted on endometriosis, however its molecular mechanism and pathogenesis are still not completely clear. The role of ELAM-1 gene in mediating the adhesion of tumor cells to endothelial cells by binding to ELAM-1 ligands expressed by eosinophils, neutrophils, monocytes, natural killer cell effectors, T lymphocyte cells, and cancer (progenitor/stem) cells is well studied. Our hypothesis proposes that the alteration in ELAM-1 mRNA level is a significant point in successful transplantation deregulations of effective migration genes, which may be related to endometrial proliferative disorders such as endometriosis.
  
  
  METHODS
  In this study, real time-PCR, Western blotting and immunohistochemistry have been used in order to determine the expression pattern of ELAM-1 gene in 22 patients. For the validation study, a control group of 8 healthy women was recruited. Comparison of the prognostic accuracy of ELAM-1 with other clinical and pathological risk factors were analyzed by logistic regression.
  
  
  RESULTS
  The endometriosis tissues from women aged 33±7 years (mean ± SEM) were assessed. The results showed that the ELAM-1 mRNA expression is significantly higher in patients group compare control group (P=0.0001). Moreover, in the samples of stages III and IV, ELAM-1 gene has expressed more (P<0.001). ELAM-1 immunostaining expressed positive staining in the patients group. ELAM-1 protein expression level was correlated directly with American Fertility Society Score and depicted no correlation with age.
  
  
  CONCLUSIONS
  According to our results, ELAM-1 upregulation may promote disorder in adhesion, and migration of the cells leading to the endometriosis disorder.
authors.affiliation: Stem Cell Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran - bahrami.hoda@gmail.com.|Academy of Gengostar, Karaj, Iran - bahrami.hoda@gmail.com.|Department of Biotechnology, Faculty of Science, Islamic Azad University, Damghan, Iran - bahrami.hoda@gmail.com.
authors.forename: Hoda
authors.initials: H
authors.lastname: Bahrami
authors.affiliation: Stem Cell Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Roozafzoon
authors.affiliation: Department of Gynecology and Obstetrics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Zinat
authors.initials: Z
authors.lastname: Ghanbari
authors.affiliation: Academy of Gengostar, Karaj, Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Jegargooshe
authors.affiliation: Department of Microbiology, Faculty of Science, Islamic Azad University, Arak, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Jafari
authors.affiliation: Department of Chemistry, Faculty of Science and Engineering, York University, Toronto, Canada.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nazari
authors.affiliation: Stem Cell Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Heidari-Keshel
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002448:Cell Adhesion; D002465:Cell Movement; D019040:E-Selectin / Q000235:genetics*; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D016015:Logistic Models; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors
chemical_list: D019040:E-Selectin; D012333:RNA, Messenger