title: Biochemical indices of oxidative stress and inflammation in the evaluation of peripheral artery disease.
abstract: The aims of this study were: (1) to investigate changes in indices of oxidative stress and inflammation in the evaluation of peripheral artery disease (PAD); (2) to compare the diagnostic efficacy of these parameters with that of classical clinical laboratory routine parameters.
  
  
  
  We studied 115 patients with PAD and 300 healthy volunteers.
  
  
  
  PAD patients had significantly increased circulating concentrations of F2-isoprostanes, protein carbonyls, chemokine (C-C motif) ligand 2 (CCL2), high-sensitivity C-reactive protein (hs-CRP), β-2-microglobulin (B2M), and decreased paraoxonase-1 (PON1) levels. When patients were classified according to the Fontaine score, we observed important increases in plasma F2-isoprostanes and CCL2 that appeared in milder stages of the disease, and remained so at similar levels in more advanced stages; almost no overlapping with the control group was noted. Receiver operating characteristics analysis comparing patients and controls revealed that the areas under the curve for F2-isoprostanes and CCL2 approached unity [0.999 (0.998-1.000) and 0.993 (0.985-1.000)], respectively, and significantly higher to those of the other measured parameters.
  
  
  
  Our data suggest that F2-isoprostanes and CCL2 measurements may be useful tools for the diagnosis of PAD.
authors.affiliation: Unitat de Recerca Biomèdica (URB-CRB), Hospital Universitari de Sant Joan, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Spain; Laboratori de Referència de Catalunya SUD, Hospital Universitari de Sant Joan, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Fort-Gallifa
authors.affiliation: Unitat de Recerca Biomèdica (URB-CRB), Hospital Universitari de Sant Joan, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: Anabel
authors.initials: A
authors.lastname: García-Heredia
authors.affiliation: Unitat de Recerca Biomèdica (URB-CRB), Hospital Universitari de Sant Joan, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Hernández-Aguilera
authors.affiliation: Laboratori de Referència de Catalunya SUD, Hospital Universitari de Sant Joan, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: Josep M
authors.initials: JM
authors.lastname: Simó
authors.affiliation: Servei d'Angiologia, Cirurgia Vascular i Endocirurgia, Hospital Universitari Joan XXIII, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Tarragona, Spain.
authors.forename: Julio
authors.initials: J
authors.lastname: Sepúlveda
authors.affiliation: Servei d'Angiologia, Cirurgia Vascular i Endocirurgia, Hospital Universitari Joan XXIII, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Tarragona, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Martín-Paredero
authors.affiliation: Unitat de Recerca Biomèdica (URB-CRB), Hospital Universitari de Sant Joan, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Spain. Electronic address: jcamps@grupsagessa.com.
authors.forename: Jordi
authors.initials: J
authors.lastname: Camps
authors.affiliation: Unitat de Recerca Biomèdica (URB-CRB), Hospital Universitari de Sant Joan, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Joven
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D058729:Peripheral Arterial Disease / Q000097:blood* / Q000175:diagnosis; D011446:Prospective Studies; D012372:ROC Curve; D055815:Young Adult
chemical_list: D015415:Biomarkers
keywords: Chemokine (C-C motif) ligand 2; F(2)-isoprostanes; Inflammation; Oxidative stress; Paraoxonase-1; Peripheral artery disease; Protein carbonyls