title: Decoration of reduced graphene oxide with rhodium nanoparticles for the design of a sensitive electrochemical enzyme biosensor for 17β-estradiol.
abstract: A novel nanocomposite material consisting of reduced graphene oxide/Rh nanoparticles was prepared by a one-pot reaction process. The strategy involved the simultaneous reduction of RhCl3and graphene oxide with NaBH4and the in situ deposition of the metal nanoparticles on the 2D carbon nanomaterial planar sheets. Glassy carbon electrode coated with this nanocomposite was employed as nanostructured support for the cross-linking of the enzyme laccase with glutaraldehyde to construct a voltammperometric biosensor for 17β-estradiol in the 0.9-11 pM range. The biosensor showed excellent analytical performance with high sensitivity of 25.7AµM-1cm-1, a very low detection limit of 0.54pM and high selectivity. The biosensor was applied to the rapid and successful determination of the hormone in spiked synthetic and real human urine samples.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of MadridMadrid28040Spain.
authors.forename: Eloy
authors.initials: E
authors.lastname: Povedano
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of MadridMadrid28040Spain; Institute of Chemistry, State University of São PauloPO Box 780São CarlosSP13560-970Brazil.
authors.forename: Fernando H
authors.initials: FH
authors.lastname: Cincotto
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of MadridMadrid28040Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: Parrado
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of MadridMadrid28040Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Díez
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of MadridMadrid28040Spain. Electronic address: alfredo_2sanchez@hotmail.com.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Sánchez
authors.affiliation: Engineering School, Mackenzie Presbiterian UniversitySão PauloSP01302-907Brazil.
authors.forename: Thiago C
authors.initials: TC
authors.lastname: Canevari
authors.affiliation: Institute of Chemistry, State University of São PauloPO Box 780São CarlosSP13560-970Brazil.
authors.forename: Sergio A S
authors.initials: SAS
authors.lastname: Machado
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of MadridMadrid28040Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Pingarrón
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of MadridMadrid28040Spain. Electronic address: rvillalonga@quim.ucm.es.
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Villalonga
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D004958:Estradiol / Q000652:urine*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D042845:Laccase / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008958:Models, Molecular; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D012238:Rhodium / Q000737:chemistry*; D055454:Trametes / Q000201:enzymology; D014159:Transducers
chemical_list: D010087:Oxides; D004958:Estradiol; D006108:Graphite; D012238:Rhodium; D042845:Laccase
keywords: 17β-estradiol; Biosensor; Graphene oxide; Nanocomposite material; Rhodium nanoparticles