title: Ultraconserved long non-coding RNA uc.63 in breast cancer.
abstract: Transcribed-ultraconserved regions (T-UCRs) are long non-coding RNAs (lncRNA) encoded by a subset of long ultraconserved stretches in the human genome. Recent studies revealed that the expression of several T-UCRs is altered in cancer and growing evidences underline the importance of T-UCRs in oncogenesis, offering also potential new strategies for diagnosis and prognosis. We found that overexpression of one specific T-UCRs named uc.63 is associated with bad outcome in luminal A subtype of breast cancer patients. uc.63 is localized in the third intron of exportin-1 gene (XPO1) and is transcribed in the same orientation of its host gene. Interestingly, silencing of uc.63 induces apoptosis in vitro. However, silencing of host gene XPO1 does not cause the same effect suggesting that the transcription of uc.63 is independent of XPO1. Our results reveal an important role of uc.63 in promoting breast cancer cells survival and offer the prospect to identify a signature associated with poor prognosis.
authors.affiliation: Medical Research Council, Toxicology Unit, Hodgkin Building, University of Leicester, Leicester, UK.
authors.forename: Alberto
authors.initials: A
authors.lastname: Marini
authors.affiliation: Department of Experimental Medicine and Surgery, University of Rome "Tor Vergata", Rome, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Lena
authors.affiliation: Department of Experimental Medicine and Surgery, University of Rome "Tor Vergata", Rome, Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Panatta
authors.affiliation: Department of Experimental Therapeutics and The Center for RNA interference and non-coding RNA, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Cristina
authors.initials: C
authors.lastname: Ivan
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, TX, USA.
authors.forename: Leng
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Bioinformatics and Computational Biology, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Han
authors.initials: H
authors.lastname: Liang
authors.affiliation: IDI-IRCCS, Biochemistry Laboratory, Rome, Italy.
authors.forename: Margherita
authors.initials: M
authors.lastname: Annicchiarico-Petruzzelli
authors.affiliation: Department of Experimental Medicine and Surgery, University of Rome "Tor Vergata", Rome, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Di Daniele
authors.affiliation: Department of Experimental Therapeutics and The Center for RNA interference and non-coding RNA, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: George A
authors.initials: GA
authors.lastname: Calin
authors.affiliation: Department of Experimental Medicine and Surgery, University of Rome "Tor Vergata", Rome, Italy.|IDI-IRCCS, Biochemistry Laboratory, Rome, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Candi
authors.affiliation: Medical Research Council, Toxicology Unit, Hodgkin Building, University of Leicester, Leicester, UK.|Department of Experimental Medicine and Surgery, University of Rome "Tor Vergata", Rome, Italy.
authors.forename: Gerry
authors.initials: G
authors.lastname: Melino
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000401:mortality; D045744:Cell Line, Tumor; D019295:Computational Biology / Q000379:methods; D017124:Conserved Sequence; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D028884:Karyopherins / Q000235:genetics; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics; D014158:Transcription, Genetic; D000097606:Exportin 1 Protein
chemical_list: D028884:Karyopherins; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D000097606:Exportin 1 Protein
keywords: T-UCRs; apoptosis; breast cancer; lncRNA; prognosis