title: High throughput-screening of animal urine samples: It is fast but is it also reliable?
abstract: Advanced analytical technologies like ultra-high-performance liquid chromatography coupled to high resolution mass spectrometry can be used for veterinary drug screening of animal urine. The technique is sufficiently robust and reliable to detect veterinary drugs in urine samples of animals where the maximum residue limit of these compounds in organs like muscle, kidney, or liver has been exceeded. The limitations and possibilities of the technique are discussed. The most critical point is the variability of the drug concentration ratio between the tissue and urine. Ways to manage the false positive and false negatives are discussed. The capability to confirm findings and the possibility of semi-targeted analysis are also addressed. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Kantonales Labor Postfach, Zürich, 8032, Switzerland.
authors.forename: Anton
authors.initials: A
authors.lastname: Kaufmann
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000191:economics / Q000379:methods*; D013058:Mass Spectrometry / Q000191:economics / Q000379:methods*; D013552:Swine; D013997:Time Factors; D016482:Urinalysis / Q000191:economics / Q000379:methods; D019155:Veterinary Drugs / Q000652:urine*
chemical_list: D019155:Veterinary Drugs
keywords: LC-HRMS; LC-MS; animal urine screening; high throughput