title: Target organs of the Manila clam Ruditapes philippinarum for studying metal accumulation and biomarkers in pollution monitoring: laboratory and in-situ transplantation experiments.
abstract: To characterize the target organs of the Manila clam Ruditapes philippinarum for use in environmental study, the accumulation of trace metals and three biomarkers was measured in different organs. Exposure with Cu and Pb carried out under laboratory conditions revealed a linear uptake of metals throughout the experimental period in each tissue. In particular, significant increase was observed in gills and mantle. The increase of intracellular reactive oxygen species showed the great potential of gills as a target tissue for both Cu and Pb exposure. The highest activity of glutathione S-transferase and their relative increase in activity were also observed in gills. Metallothionein-like protein levels, however, increased greatly in the digestive gland and mantle during Cu and Pb exposure, respectively, although all tissues, except the foot, showed significant changes after 24 h of metal exposure. In the field study, the highest concentration of metals was recorded in the gills and mantle, accounting for over 50 % of the total accumulated metal in all sites. Additionally, Cu and Pb increased significantly in these two organs, respectively. However, the order of accumulation rate in laboratory exposure was not concomitant with those of the lab-based study, suggesting that different routes of metal uptake and exposure duration induce distinct partitioning of metals and regulating system in R. philippinarum. These series of exposure studies demonstrated that gills, mantle, and digestive gland in R. philippinarum are potential target tissues in environmental monitoring study using metal concentrations and biomarkers.
authors.affiliation: Marine Chemistry & Geochemistry Research Center, Korea Institute of Ocean Science and Technology (KIOST), Ansan, 15627, Republic of Korea.
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Won
authors.affiliation: Marine Chemistry & Geochemistry Research Center, Korea Institute of Ocean Science and Technology (KIOST), Ansan, 15627, Republic of Korea.|Department of Integrated Ocean Sciences, Korea University of Science and Technology (UST), Ansan, 15627, Republic of Korea.
authors.forename: Kyung-Tae
authors.initials: KT
authors.lastname: Kim
authors.affiliation: Marine Chemistry & Geochemistry Research Center, Korea Institute of Ocean Science and Technology (KIOST), Ansan, 15627, Republic of Korea.
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Ocean Observation & Information Section, Korea Institute of Ocean Science and Technology (KIOST), Ansan, 15627, Republic of Korea.
authors.forename: Eun-Soo
authors.initials: ES
authors.lastname: Kim
authors.affiliation: Marine Chemistry & Geochemistry Research Center, Korea Institute of Ocean Science and Technology (KIOST), Ansan, 15627, Republic of Korea. ktra@kiost.ac.kr.|Department of Integrated Ocean Sciences, Korea University of Science and Technology (UST), Ansan, 15627, Republic of Korea. ktra@kiost.ac.kr.
authors.forename: Kongtae
authors.initials: K
authors.lastname: Ra
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D049872:Bivalvia / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D004064:Digestive System / Q000737:chemistry* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D005880:Gills / Q000737:chemistry* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D060106:Lakes / Q000737:chemistry; D008668:Metallothionein / Q000378:metabolism; D019216:Metals, Heavy / Q000032:analysis / Q000378:metabolism*; D056910:Republic of Korea; D014018:Tissue Distribution; D014131:Trace Elements / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D019216:Metals, Heavy; D014131:Trace Elements; D014874:Water Pollutants, Chemical; D008668:Metallothionein; D005982:Glutathione Transferase
keywords: Bioaccumulation; Glutathione S-transferase; Manila clam; Metallothionein-like proteins (MTLPs); Reactive oxygen species; Ruditapes philippinarum; Trace metal