title: RNA expression of TLR10 in normal equine tissues.
abstract: BACKGROUND
  Toll like receptors are one of the major innate immune system pathogen recognition systems. There is little data on the expression of the TLR10 member of this family in the horse.
  
  
  RESULTS
  This paper describes the genetic structure of the Equine TLR10 gene and its RNA expression in a range of horse tissues. It describes the phylogenetic analysis of the Equine TLR1,6,10,2 annotations in the horse genome, firmly identifying them in their corresponding gene clades compared to other species and firmly placing the horse gene with other TLR10 genes from odd-toed ungulates. Additional 3' transcript extensions to that annotated for TLR10 in the horse genome have been identified by analysis of RNAseq data. RNA expression of the equine TLR10 gene was highest in peripheral blood mononucleocytes and lymphoid tissue (lymph nodes and spleen), however some expression was detected in all tissues tested (jejunum, caudal mesenteric lymph nodes, bronchial lymph node, spleen, lung, colon, kidney and liver). Additional data on RNAseq expression of all equine TLR genes (1-4 and 6-10) demonstrate higher expression of TLR4 than other equine TLRs in all tissues.
  
  
  CONCLUSIONS
  The equine TLR10 gene displays significant homology to other mammalian TLR10 genes and could be reasonably assumed to have similar fuctions. Its RNA level expression is higher in resting state PBMCs in horses than in other tissues.
authors.affiliation: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK. rachael.tarlinton@nottingham.ac.uk.
authors.forename: Rachael E
authors.identifier: http://orcid.org/0000-0003-3325-2311
authors.initials: RE
authors.lastname: Tarlinton
authors.affiliation: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
authors.forename: Lauren
authors.initials: L
authors.lastname: Alder
authors.affiliation: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.|Advanced Data Analysis Centre, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
authors.forename: Joanna
authors.initials: J
authors.lastname: Moreton
authors.affiliation: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
authors.forename: Grazieli
authors.initials: G
authors.lastname: Maboni
authors.affiliation: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.|Advanced Data Analysis Centre, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Emes
authors.affiliation: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
authors.forename: Sabine
authors.initials: S
authors.lastname: Tötemeyer
mesh_terms: D000818:Animals; D015870:Gene Expression; D016678:Genome; D006736:Horses / Q000145:classification / Q000235:genetics* / Q000276:immunology; D007113:Immunity, Innate; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics* / Q000276:immunology; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D051201:Toll-Like Receptor 10 / Q000235:genetics* / Q000276:immunology
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D051201:Toll-Like Receptor 10
keywords: Equus caballus; Horse; RNA; TLR10; Toll-like receptor