title: Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy.
abstract: A cavity ring-down spectroscopy (CRDS) instrument was developed using mature, robust hardware for the measurement of carbon-14 in biological studies. The system was characterized using carbon-14 elevated glucose samples and returned a linear response up to 387 times contemporary carbon-14 concentrations. Carbon-14 free and contemporary carbon-14 samples with varying carbon-13 concentrations were used to assess the method detection limit of approximately one-third contemporary carbon-14 levels. Sources of inaccuracies are presented and discussed, and the capability to measure carbon-14 in biological samples is demonstrated by comparing pharmacokinetics from carbon-14 dosed guinea pigs analyzed by both CRDS and accelerator mass spectrometry. The CRDS approach presented affords easy access to powerful carbon-14 tracer techniques that can characterize complex biochemical systems.
authors.affiliation: Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory , 7000 East Avenue, Livermore, California 94550, United States.
authors.forename: A Daniel
authors.initials: AD
authors.lastname: McCartt
authors.affiliation: Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory , 7000 East Avenue, Livermore, California 94550, United States.
authors.forename: Ted J
authors.initials: TJ
authors.lastname: Ognibene
authors.affiliation: Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory , 7000 East Avenue, Livermore, California 94550, United States.
authors.forename: Graham
authors.initials: G
authors.lastname: Bench
authors.affiliation: Biology and Biotechnology Division, Lawrence Livermore National Laboratory , 7000 East Avenue, Livermore, California 94550, United States.
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Turteltaub
mesh_terms: D002250:Carbon Radioisotopes; D005947:Glucose / Q000032:analysis*; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D002250:Carbon Radioisotopes; D005947:Glucose