title: Metagenomic signatures of a tropical mining-impacted stream reveal complex microbial and metabolic networks.
abstract: Bacteria from aquatic ecosystems significantly contribute to biogeochemical cycles, but details of their community structure in tropical mining-impacted environments remain unexplored. In this study, we analyzed a bacterial community from circumneutral-pH tropical stream sediment by 16S rRNA and shotgun deep sequencing. Carrapatos stream sediment, which has been exposed to metal stress due to gold and iron mining (21 [g Fe]/kg), revealed a diverse community, with predominance of Proteobacteria (39.4%), Bacteroidetes (12.2%), and Parcubacteria (11.4%). Among Proteobacteria, the most abundant reads were assigned to neutrophilic iron-oxidizing taxa, such as Gallionella, Sideroxydans, and Mariprofundus, which are involved in Fe cycling and harbor several metal resistance genes. Functional analysis revealed a large number of genes participating in nitrogen and methane metabolic pathways despite the low concentrations of inorganic nitrogen in the Carrapatos stream. Our findings provide important insights into bacterial community interactions in a mining-impacted environment.
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil.
authors.forename: Mariana P
authors.initials: MP
authors.lastname: Reis
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil.
authors.forename: Marcela F
authors.initials: MF
authors.lastname: Dias
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil.
authors.forename: Patrícia S
authors.initials: PS
authors.lastname: Costa
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil.
authors.forename: Marcelo P
authors.initials: MP
authors.lastname: Ávila
authors.affiliation: Centro de Pesquisas René Rachou Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, Belo Horizonte, Minas Gerais 30190-002, Brazil.
authors.forename: Laura R
authors.initials: LR
authors.lastname: Leite
authors.affiliation: Centro de Pesquisas René Rachou Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, Belo Horizonte, Minas Gerais 30190-002, Brazil.
authors.forename: Flávio M G
authors.initials: FMG
authors.lastname: de Araújo
authors.affiliation: Centro de Pesquisas René Rachou Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, Belo Horizonte, Minas Gerais 30190-002, Brazil.
authors.forename: Anna C M
authors.initials: ACM
authors.lastname: Salim
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil.
authors.forename: Mônica
authors.initials: M
authors.lastname: Bucciarelli-Rodriguez
authors.affiliation: Centro de Pesquisas René Rachou Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, Belo Horizonte, Minas Gerais 30190-002, Brazil; Instituto Tecnológico Vale, Rua Boaventura da Silva 955, Belém, Pará 66055-090, Brazil.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Oliveira
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil.
authors.forename: Edmar
authors.initials: E
authors.lastname: Chartone-Souza
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil. Electronic address: amaral@ufmg.br.
authors.forename: Andréa M A
authors.initials: AMA
authors.lastname: Nascimento
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000378:metabolism; D001938:Brazil; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology; D007501:Iron / Q000032:analysis / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000187:drug effects*; D056186:Metagenomics; D008906:Mining; D010802:Phylogeny; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000737:chemistry / Q000382:microbiology; D014329:Tropical Climate; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism
chemical_list: D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D007501:Iron
keywords: 16S rRNA; Freshwater; Iron-rich sediment; Shotgun metagenome