title: LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo.
abstract: The present study intended to demonstrate the effects of long noncoding RNA growth arrest-specific transcript 5 (GAS5) on the migration and invasion of melanoma cells. We first detected the expression of GAS5 among four kinds of melanoma cell lines, followed by constructing GAS5-knocked down and overexpressed stable cells. Next, we evaluated the effects of GAS5 on cell migration and invasion using wound healing and gelatin zymography assays. Finally, melanoma cells with different GAS5 expression were injected into nude mice, and the tumor volumes were recorded and tumor tissues were analyzed after sacrificing the mice. This study systematically examined the function of GAS5 in mediating melanoma metastasis and revealed that GAS5 plays an anticancer role in melanoma via regulating gelatinase A and B, both in vitro and in vivo.
authors.affiliation: Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan, People's Republic of China; PET/CT Center, Yunan Tumor Hospital, the Third Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Huixin
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Orthopaedics, Yunan Tumor Hospital, the Third Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Pharmacy, the Second Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Qiaoqiao
authors.initials: Q
authors.lastname: Han
authors.affiliation: Department of Orthopaedics, Yunan Tumor Hospital, the Third Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Junping
authors.initials: J
authors.lastname: Fu
authors.affiliation: Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Yuye
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Yuechun
authors.initials: Y
authors.lastname: Zhu
keywords: GAS5; gelatinase; lncRNA; melanoma; metastasis