title: Transcriptomic profiling revealed the signatures of intestinal barrier alteration and pathogen entry in turbot (Scophthalmus maximus) following Vibrio anguillarum challenge.
abstract: The mucosal immune system serves as the frontline barriers of host defense against pathogen infection, especially for the fishes, which are living in the pathogen rich aquatic environment. The intestine constitutes the largest surface body area in constantly contact with the external pathogens, and plays a vital role in the immune defense against inflammation and pathogen infection. Previous studies have revealed that fish intestine might serves as the portal of entry for Vibrio anguillarum. To characterize the immune actors and their associated immune activities in turbot intestine barrier during bacterial infection, here we examined the gene expression profiles of turbot intestine at three time points following experimental infection with V. anguillarum utilizing RNA-seq technology. A total of 122 million reads were assembled into 183,101 contigs with an average length of 1151 bp and the N50 size of 2302 bp. Analysis of differential gene expression between control and infected samples at 1 h, 4 h, and 12 h revealed 2079 significantly expressed genes. Enrichment and pathway analysis of the differentially expressed genes showed the centrality of the pathogen attachment and recognition, antioxidant/apoptosis, mucus barrier modification and immune activation/inflammation in the pathogen entry and host immune responses. The present study reported the novel gene expression patterns in turbot mucosal immunity, which were overlooked in previous studies. Our results can help to understand the mechanisms of turbot host defense, and may also provide foundation to identify the biomarkers for future selection of disease-resistant broodstock and evaluation of disease prevention and treatment options.
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, 200062, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Ministry of Agriculture Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, China; National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, China.
authors.forename: Baofeng
authors.initials: B
authors.lastname: Su
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Fengqiao
authors.initials: F
authors.lastname: Liu
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Song
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Yichao
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Dong
authors.affiliation: School of International Education and Exchange, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Tan
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China. Electronic address: leoochao@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D001422:Bacterial Adhesion; D002478:Cells, Cultured; D005412:Flatfishes / Q000276:immunology*; D018928:Immunity, Mucosal; D007249:Inflammation / Q000235:genetics / Q000276:immunology*; D007422:Intestines / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D009093:Mucus / Q000378:metabolism; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000276:immunology; D051192:Receptors, Pattern Recognition / Q000235:genetics; D059467:Transcriptome; D014735:Vibrio Infections / Q000276:immunology*
chemical_list: D000975:Antioxidants; D000069452:Pathogen-Associated Molecular Pattern Molecules; D051192:Receptors, Pattern Recognition
keywords: Immune response; Intestine; RNA-seq; Turbot; Vibrio anguillarum