title: Zinc Levels in Seminal Fluid in Infertile Males and its Relation with Serum Free Testosterone.
abstract: BACKGROUND
  The role of zinc is critical to reproduction potential. Seminal zinc is thought to be derived almost exclusively from prostatic secretions. Sperm motility is significantly influenced by zinc. Zinc deficiency has been linked with male sterility and subfertility.
  
  
  OBJECTIVE
  To assess the influence of seminal plasma zinc on seminogram characteristics and whether endogenous testosterone affects the seminal levels of zinc.
  
  
  METHODS
  The semen samples were obtained from 150 male partners of infertile couples who attended the Reproductive Biology Unit of the Department of Physiology, within the age 21-50 years and semen samples were analysed for the routine seminogram parameters. All the subjects were classified into two main groups, A- the subjects with normal ejaculates (n=62) and B- the subjects with abnormal ejaculates, who were further sub divided into the following groups: i) Asthenoteratozoospermics (n=43); ii) Oligoasthenoteratozoospermics (n=24); and iii) Azoospermics (n=21). The seminal plasma zinc was measured spectrophotometrically. The sample for serum free testosterone was sent to Thyrocare laboratory.
  
  
  RESULTS
  The seminal plasma zinc was found to be significantly lower in the abnormal ejaculates than in the normal ejaculates. A statistically significant positive correlation was observed between the seminal plasma zinc and serum free testosterone (p<0.05, r=0.449). Statistically significant correlation was also found between seminal plasma zinc and all the seminogram parameters such as the sperm concentration, sperm motility and sperm morphology (p<0.05, r= 0.86, 0.87 and 0.86 respectively).
  
  
  CONCLUSIONS
  Low seminal plasma zinc might be a significant causative factor in impairing sperm functions and its dependence on endogenous free testosterone, is observed from a positive correlation between the two.
authors.affiliation: Tutor, Department of Physiology, Mahatma Gandhi Institute of Medical Sciences , Sevagram, Wardha, Maharashtra, India.
authors.forename: Radhika Purushottam
authors.initials: RP
authors.lastname: Kothari
authors.affiliation: Professor, Department of Physiology, Mahatma Gandhi Institute of Medical Sciences , Sevagram, Wardha, Maharashtra, India .
authors.forename: Ajay Rajeshwar
authors.initials: AR
authors.lastname: Chaudhari
keywords: Male infertility; Seminal zinc; Seminogram; Spectrophotometric method