title: Effect of Water-Glass Coating on HA and HA-TCP Samples for MSCs Adhesion, Proliferation, and Differentiation.
abstract: Ca-P and silicon based materials have become very popular as bone tissue engineering materials. In this study, water-glass (also known as sodium silicate glass) was coated on sintered hydroxyapatite (HA) and HA-TCP (TCP stands for tricalcium phosphate) samples and subsequently heat-treated at 600°C for 2 hrs. X-rays diffraction showed the presence of β- and α-TCP phases along with HA in the HA-TCP samples. Samples without coating, with water-glass coating, and heat-treated after water-glass coating were used to observe the adhesion and proliferation response of bone marrow derived-mesenchymal stem cells (MSCs). Cell culture was carried out for 4 hrs, 1 day, and 7 days. Interestingly, all samples showed similar response for cell adhesion and proliferation up to 7-day culture but fibronectin, E-cadherin, and osteogenic differentiation related genes (osteocalcin and osteopontin) were significantly induced in heat-treated water-glass coated HA-TCP samples. A water-glass coating on Ca-P samples was not found to influence the cell proliferation response significantly but activated some extracellular matrix genes and induced osteogenic differentiation in the MSCs.
authors.affiliation: Materials Science and Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
authors.forename: Indu
authors.initials: I
authors.lastname: Bajpai
authors.affiliation: Materials Science and Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
authors.forename: Duk Yeon
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Anatomy, College of Medicine, Yeungnam University, 317-1 Daemyung-Dong, Daegu 705-717, Republic of Korea.
authors.forename: Jung
authors.initials: J
authors.lastname: Kyong-Jin
authors.affiliation: Department of Anatomy, College of Medicine, Yeungnam University, 317-1 Daemyung-Dong, Daegu 705-717, Republic of Korea.
authors.forename: In-Hwan
authors.initials: IH
authors.lastname: Song
authors.affiliation: Materials Science and Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
authors.forename: Sukyoung
authors.identifier: 0000-0002-1382-2041
authors.initials: S
authors.lastname: Kim
mesh_terms: D001854:Bone Marrow Cells / Q000187:drug effects; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005898:Glass / Q000737:chemistry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000096:biosynthesis; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology; D023822:Tissue Engineering; D014867:Water
chemical_list: D002130:Calcium Phosphates; D017640:Silicates; D014867:Water; D015675:Osteocalcin; D053495:Osteopontin; D017886:Durapatite; C005691:sodium silicate; C018392:tricalcium phosphate