title: Mitochondria-targeted antioxidant mitotempo protects mitochondrial function against amyloid beta toxicity in primary cultured mouse neurons.
abstract: Mitochondrial defects including excess reactive oxygen species (ROS) production and compromised ATP generation are featured pathology in Alzheimer's disease (AD). Amyloid beta (Aβ)-mediated mitochondrial ROS overproduction disrupts intra-neuronal Redox balance, in turn exacerbating mitochondrial dysfunction leading to neuronal injury. Previous studies have found the beneficial effects of mitochondria-targeted antioxidants in preventing mitochondrial dysfunction and neuronal injury in AD animal and cell models, suggesting that mitochondrial ROS scavengers hold promise for the treatment of this neurological disorder. In this study, we have determined that mitotempo, a novel mitochondria-targeted antioxidant protects mitochondrial function from the toxicity of Aβ in primary cultured neurons. Our results showed that Aβ-promoted mitochondrial superoxide production and neuronal lipid oxidation were significantly suppressed by the application of mitotempo. Moreover, mitotempo also demonstrated protective effects on mitochondrial bioenergetics evidenced by preserved mitochondrial membrane potential, cytochrome c oxidase activity as well as ATP production. In addition, the Aβ-induced mitochondrial DNA (mtDNA) depletion and decreased expression levels of mtDNA replication-related DNA polymerase gamma (DNA pol γ) and Twinkle were substantially mitigated by mitotempo. Therefore, our study suggests that elimination of excess mitochondrial ROS rescues mitochondrial function in Aβ-insulted neruons; and mitotempo has the potential to be a promising therapeutic agent to protect mitochondrial and neuronal function in AD.
authors.affiliation: Department of Neurology, Beijing Jishuitan Hospital, 31 Xinjiekou E Rd, Xicheng, Beijing, 100035, China. Electronic address: hhtsd@163.com.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Neurology, Beijing Jishuitan Hospital, 31 Xinjiekou E Rd, Xicheng, Beijing, 100035, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Li
mesh_terms: D016229:Amyloid beta-Peptides / Q000008:administration & dosage*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D051379:Mice; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D009943:Organophosphorus Compounds / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010880:Piperidines / Q000008:administration & dosage*
chemical_list: D016229:Amyloid beta-Peptides; D000975:Antioxidants; C555916:MitoTEMPO; D009943:Organophosphorus Compounds; D010880:Piperidines
keywords: Alzheimer's disease; Amyloid beta; Mitochondria-targeted antioxidant; Mitochondrial dysfunction; Mitotempo