title: Isolation and characterization of Helicobacter pylori recovered from gastric biopsies under anaerobic conditions.
abstract: OBJECTIVE
  Helicobacter pylori can survive long incubation periods under anaerobic conditions, and should be possible to isolate under anaerobic conditions. Our aim was to isolate H. pylori in anaerobic conditions, from gastric biopsies of H. pylori infected patients.
  
  
  METHODS
  We enrolled 27 patients with bleeding (erosive) gastritis (mean age 36.3 years, 55.6% male) from Hanoi, Vietnam. H. pylori status was confirmed by qPCR.
  
  
  RESULTS
  H. pylori were recovered under anaerobic and micro-aerobic conditions from gastric biopsies in 16 patients. Anaerobic conditions yielded significantly higher H. pylori recovery rates than micro-aerobic conditions (81.3% vs. 31.3%, P= 0.01). H. pylori isolates were characterized by PCR for specific virulence markers and the genotypes were similar to those previously described in this region of the world.
  
  
  CONCLUSIONS
  H. pylori can be isolated under anaerobic conditions. These findings may provide new insight into the physiology of this human pathogen and help to identify the route of H. pylori transmission.
authors.affiliation: Department of Medicine, NYU Langone Medical Center, New York, NY; Department Microbiology, NYU Langone Medical Center, New York, NY. Electronic address: Perezg02@med.nyu.edu.
authors.forename: Guillerm Ignacio
authors.initials: GI
authors.lastname: Perez-Perez
authors.affiliation: Buu Dien Hospital, Hanoi, Vietnam.
authors.forename: Thinh Nguyen
authors.initials: TN
authors.lastname: Van
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Sciences and Technology.
authors.forename: Duong
authors.initials: D
authors.lastname: Thu Huong
authors.affiliation: Department of Medicine, NYU Langone Medical Center, New York, NY.
authors.forename: Gao
authors.initials: G
authors.lastname: Zhan
authors.affiliation: E Hospital, Hanoi, Vietnam.
authors.forename: Do
authors.initials: D
authors.lastname: Nguyet Anh
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Sciences and Technology.
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Nguyet
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Sciences and Technology.
authors.forename: Loan Ta
authors.initials: LT
authors.lastname: Thi
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Sciences and Technology.
authors.forename: Nguyen
authors.initials: N
authors.lastname: Van Thinh
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Sciences and Technology; Research and Development Center for Biotechnology CBT, VUSTA.
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Hong-Hanh
mesh_terms: D000328:Adult; D000693:Anaerobiosis; D001431:Bacteriological Techniques / Q000379:methods*; D001706:Biopsy; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology*; D005838:Genotype; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014744:Vietnam; D037521:Virulence Factors / Q000032:analysis / Q000235:genetics
chemical_list: D037521:Virulence Factors
keywords: Anaerobic conditions; Bacterial culture; H. pylori isolation