title: Diversity of insulin-like peptide signaling system proteins in Calanus finmarchicus (Crustacea; Copepoda) - Possible contributors to seasonal pre-adult diapause.
abstract: Calanus finmarchicus, an abundant calanoid copepod in the North Atlantic Ocean, is both a major grazer on phytoplankton and an important forage species for invertebrate and vertebrate predators. One component of the life history of C. finmarchicus is the overwintering dormancy of sub-adults, a feature key for the annual recruitment of this species in early spring. While little is known about the control of dormancy in C. finmarchicus, one hypothesis is that it is an insect-like diapause, where the endocrine system is a key regulator. One group of hormones implicated in the control of insect diapause is the insulin-like peptides (ILPs). Here, C. finmarchicus transcriptomic data were used to predict ILP signaling pathway proteins. Four ILP precursors were identified, each possessing a distinct A- and B-chain peptide; these peptides are predicted to form bioactive heterodimers via inter-chain disulfide bridging. Two ILP receptors, which likely represent splice variants of a common gene, were identified. Three insulin-degrading enzymes were also discovered, as were proteins encoding the transcription factor FOXO, a downstream target of ILP that has been implicated in the regulation of insect diapause, and insulin receptor substrate, a protein putatively linking the ILP receptor and FOXO. RNA-Seq data suggest that some C. finmarchicus insulin pathway transcripts are differentially expressed across development. As in insects, the ILP signaling system may be involved in controlling C. finmarchicus' organism-environment interactions (e.g., regulation of seasonal sub-adult diapause), a hypothesis that can now be investigated using these data.
authors.affiliation: Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, School of Ocean and Earth Science and Technology, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA. Electronic address: crabman@pbrc.hawaii.edu.
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Christie
authors.affiliation: Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, School of Ocean and Earth Science and Technology, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.
authors.forename: Vittoria
authors.initials: V
authors.lastname: Roncalli
authors.affiliation: Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, School of Ocean and Earth Science and Technology, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.
authors.forename: Petra H
authors.initials: PH
authors.lastname: Lenz
mesh_terms: D000818:Animals; D033342:Copepoda / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D008675:Metamorphosis, Biological; D011506:Proteins / Q000378:metabolism*
chemical_list: D007328:Insulin; C084797:Leydig insulin-like protein; D011506:Proteins
keywords: Bioinformatics; Forkhead box protein O (FOXO); Insulin receptor substrate (IRS); Insulin-degrading enzyme (IDE); Insulin-like peptide (ILP); Insulin-like peptide receptor (ILPR); Transcriptomics