title: Fluorogenic Enhancement of an in Vitro-Selected Peptide Ligand by Replacement of a Fluorescent Group.
abstract: To prepare a fluorogenic peptide ligand which binds to an arbitrary target, we previously succeeded in seeking a fluorogenic ligand to calmodulin using in vitro selection. In this study the environment-sensitive fluorescent group in the selected peptide ligand was replaced with other fluorescent groups to find the possibility to increase the fluorogenic activity. Surface plasmon resonance measurement exhibited that the binding affinity was held even after the replacement. However, the replacement significantly affected the fluorogenic activity. It depended on the kind of incorporated fluorophors and linker length. As a result, the incorporation of 4-N,N-dimethylamino-1,8-naphthalimide enhanced the fluorescence intensity over 100-fold in the presence of target calcium-bound calmodulin. This study demonstrated that the functionality of in vitro selected peptide can be tuned with keeping the binding affinity.
authors.affiliation: High Magnetic Field Laboratory, Chinese Academy of Sciences , Hefei, Anhui 230031, P. R. China.|Nano Medical Engineering Laboratory, RIKEN , 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN , 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Laboratory for Computational Molecular Design, Computational Biology Research Core, RIKEN Quantitative Biology Center , 2F, QBiC Building B, 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Hirano
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN , 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.|Graduate School of Science and Engineering, Saitama University , 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, Japan.
authors.forename: Marziyeh
authors.initials: M
authors.lastname: Kariminavargani
authors.affiliation: Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Tada
authors.affiliation: Key Laboratory of Organic Solids, Beijing National Laboratory of Molecular Science, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors.forename: Guanxin
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN , 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.|Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Uzawa
authors.affiliation: Key Laboratory of Organic Solids, Beijing National Laboratory of Molecular Science, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors.forename: Deqing
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Graduate School of Science and Engineering, Saitama University , 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Hirose
authors.affiliation: Laboratory for Computational Molecular Design, Computational Biology Research Core, RIKEN Quantitative Biology Center , 2F, QBiC Building B, 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Taiji
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN , 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.|Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D002147:Calmodulin / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008024:Ligands; D053644:Naphthalimides / Q000138:chemical synthesis / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: C533757:4-N,N-dimethylamino-1,8-naphthalimide; D002147:Calmodulin; D005456:Fluorescent Dyes; D008024:Ligands; D053644:Naphthalimides; D010455:Peptides