title: Correlation between FOXP3 expression and gastric cancer.
abstract: The aim of the present study was to investigate the expression and function of forkhead box protein 3 (FOXP3) in gastric cancer using a rat model. A total of 92 Wistar rats were divided into two groups: An experimental group (n=46) and a control group (n=46). In the experimental group, sarcosine ethyl ester hydrochloride and sodium nitrite carcinogens were administered for 6 months to induce gastric cancer, whereas the control group was administered saline. Reverse transcription-polymerase chain reaction, immunoblotting, immunohistochemistry and western blotting were applied to analyze FOXP3 expression in gastric cancer and normal gastric tissue in the experimental and control groups, respectively. The association between FOXP3 expression and gastric cancer pathogenesis was investigated. In the experimental group, 6/46 rats developed hyperplastic lesions (grade I), 8 rats developed precancerous lesions (grade II), 18 rats developed early stage gastric cancer (grade III) and 14 rats developed gastrointestinal invasive carcinoma (grade IV). FOXP3 transcription and expression was observed in all gastric tissues of the experimental group. FOXP3 transcription and expression levels were significantly higher in the experimental group than in the control group (P<0.05). Furthermore, in the experimental group, a higher lesion grade was associated with a higher level of FOXP3 transcription and expression (P<0.05). FOXP3 protein was predominantly distributed in the tumor nuclei of the gastric cancer tissues. In the 32 pathological slices of gastric cancer tissue obtained from the experimental group, 20 cases (62.50%) exhibited positive FOXP3 staining. In the hyperplastic (grade I) and precancerous gastric (grade II) tissues, 2 cases (33.33%) and 4 cases (50.00%) exhibited positive FOXP3 staining, respectively. However, no positive FOXP3 expression was identified in the 46 pathological gastric tissue slices obtained from the control group. In conclusion, the expression of FOXP3 exhibits a positive correlation with gastric lesion grade. Therefore, FOXP3 may exhibit an important function in the occurrence and development of gastric cancer.
authors.affiliation: Department of General Surgery, Henan University Huaihe Hospital, Kaifeng, Henan 475000, P.R. China.
authors.forename: Guoxiao
authors.initials: G
authors.lastname: Guo
authors.affiliation: Department of General Surgery, Henan University Huaihe Hospital, Kaifeng, Henan 475000, P.R. China.
authors.forename: Zhikuan
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of General Surgery, Henan University Huaihe Hospital, Kaifeng, Henan 475000, P.R. China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Shi
keywords: correlation; forkhead box protein 3; gastric carcinoma