title: Impact of the Maturation of Human Primary Bone-Forming Cells on Their Behavior in Acute or Persistent Staphylococcus aureus Infection Models.
abstract: Staphylococcus aureus is one of the most frequently involved pathogens in bacterial infections such as skin abscess, pneumonia, endocarditis, osteomyelitis, and implant-associated infection. As for bone homeostasis, it is partly altered during infections by S. aureus by the induction of various responses from osteoblasts, which are the bone-forming cells responsible for extracellular matrix synthesis and its mineralization. Nevertheless, bone-forming cells are a heterogeneous population with different stages of maturation and the impact of the latter on their responses toward bacteria remains unclear. We describe the impact of S. aureus on two populations of human primary bone-forming cells (HPBCs) which have distinct maturation characteristics in both acute and persistent models of interaction. Cell maturation did not influence the internalization and survival of S. aureus inside bone-forming cells or the cell death related to the infection. By studying the expression of chemokines, cytokines, and osteoclastogenic regulators by HPBCs, we observed different profiles of chemokine expression according to the degree of cell maturation. However, there was no statistical difference in the amounts of proteins released by both populations in the presence of S. aureus compared to the non-infected counterparts. Our findings show that cell maturation does not impact the behavior of HPBCs infected with S. aureus and suggest that the role of bone-forming cells may not be pivotal for the inflammatory response in osteomyelitis.
authors.affiliation: EA 4691 ≪Biomatériaux et Inflammation en Site Osseux ≫, Pôle Santé, Université de Reims Champagne-ArdenneReims, France; UFR Pharmacie, Pôle Santé, Université de Reims Champagne-ArdenneReims, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Josse
authors.affiliation: EA 4691 ≪Biomatériaux et Inflammation en Site Osseux ≫, Pôle Santé, Université de Reims Champagne-ArdenneReims, France; UFR Odontologie, Pôle Santé, Université de Reims Champagne-ArdenneReims, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Guillaume
authors.affiliation: EA 4691 ≪Biomatériaux et Inflammation en Site Osseux ≫, Pôle Santé, Université de Reims Champagne-Ardenne Reims, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Bour
authors.affiliation: EA 4691 ≪Biomatériaux et Inflammation en Site Osseux ≫, Pôle Santé, Université de Reims Champagne-Ardenne Reims, France.
authors.forename: Flora
authors.initials: F
authors.lastname: Lemaire
authors.affiliation: EA 4691 ≪Biomatériaux et Inflammation en Site Osseux ≫, Pôle Santé, Université de Reims Champagne-ArdenneReims, France; UFR Pharmacie, Pôle Santé, Université de Reims Champagne-ArdenneReims, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Mongaret
authors.affiliation: EA 4691 ≪Biomatériaux et Inflammation en Site Osseux ≫, Pôle Santé, Université de Reims Champagne-ArdenneReims, France; UFR Odontologie, Pôle Santé, Université de Reims Champagne-ArdenneReims, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Draux
authors.affiliation: EA 4691 ≪Biomatériaux et Inflammation en Site Osseux ≫, Pôle Santé, Université de Reims Champagne-ArdenneReims, France; UFR Odontologie, Pôle Santé, Université de Reims Champagne-ArdenneReims, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Velard
authors.affiliation: EA 4691 ≪Biomatériaux et Inflammation en Site Osseux ≫, Pôle Santé, Université de Reims Champagne-ArdenneReims, France; UFR Pharmacie, Pôle Santé, Université de Reims Champagne-ArdenneReims, France.
authors.forename: Sophie C
authors.initials: SC
authors.lastname: Gangloff
mesh_terms: D002113:Calcification, Physiologic; D016923:Cell Death; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism; D024042:Collagen Type I; D016207:Cytokines / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D054884:Host-Pathogen Interactions; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000378:metabolism* / Q000382:microbiology*; D015675:Osteocalcin; D010019:Osteomyelitis / Q000378:metabolism / Q000382:microbiology; D013203:Staphylococcal Infections / Q000150:complications* / Q000276:immunology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000472:pathogenicity*
chemical_list: D018925:Chemokines; D024042:Collagen Type I; D016207:Cytokines; D015675:Osteocalcin; D003907:Dexamethasone
keywords: Staphylococcus aureus; chemokine; cytokine; differentiation; maturation; osteoblast