title: Stem cell labeling with iron oxide nanoparticles: impact of 3D culture on cell labeling maintenance.
abstract: We aimed to analyze the suitability of nanoparticles (M4E) for safe human mesenchymal stem cell (hMSC) labeling and determined cell labeling maintenance in 2D and 3D culture.
  
  
  
  We investigated cell-particle interaction and the particles' impact on cell viability, growth and proliferation. We analyzed cell labeling maintenance in 2D and 3D culture invasively and noninvasively.
  
  
  
  M4E do not affect cell viability, growth and proliferation and do not cause chromosomal aberrations. Cell labeling maintenance is up to five-times higher in 3D conditions compared with 2D culture.
  
  
  
  M4E allow safe hMSC labeling and noninvasive identification. Our hMSC-loaded, 3D tissue-engineered construct could serve as a graft for regenerative therapies, in which M4E-labeled hMSCs can migrate to their target.
authors.affiliation: Fraunhofer Institute for Interfacial Engineering & Biotechnology IGB, Translational Center "Regenerative Therapies for Oncology & Musculoskeletal Diseases" - Würzburg branch, Röntgenring 11, 97070 Würzburg, Germany.
authors.forename: Teresa
authors.initials: T
authors.lastname: Kilian
authors.affiliation: Research Center Magnetic-Resonance-Bavaria, Am Hubland, 97074 Würzburg, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Fidler
authors.affiliation: Department of Oral & Maxillofacial Surgery, Facial Plastic Surgery, Rostock University Medical Center, Schillingallee 35, 18057 Rostock, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Kasten
authors.affiliation: Tissue Engineering & Regenerative Medicine, University Hospital Würzburg, Röntgenring 11, 97070 Würzburg, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Nietzer
authors.affiliation: Tissue Engineering & Regenerative Medicine, University Hospital Würzburg, Röntgenring 11, 97070 Würzburg, Germany.
authors.forename: Veronika
authors.initials: V
authors.lastname: Landgraf
authors.affiliation: Tissue Engineering & Regenerative Medicine, University Hospital Würzburg, Röntgenring 11, 97070 Würzburg, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Weiß
authors.affiliation: Fraunhofer Institute for Interfacial Engineering & Biotechnology IGB, Translational Center "Regenerative Therapies for Oncology & Musculoskeletal Diseases" - Würzburg branch, Röntgenring 11, 97070 Würzburg, Germany.|Tissue Engineering & Regenerative Medicine, University Hospital Würzburg, Röntgenring 11, 97070 Würzburg, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Walles
authors.affiliation: Micromod Partikeltechnologie GmbH, Friedrich-Barnewitz-Straße 4, 18119 Rostock, Germany.
authors.forename: Fritz
authors.initials: F
authors.lastname: Westphal
authors.affiliation: Department of Oto-Rhino-Laryngology, Plastic, Aesthetic & Reconstructive Head & Neck Surgery, University Hospital Würzburg, Josef-Schneider-Straße 11, 97080 Würzburg, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Hackenberg
authors.affiliation: Micromod Partikeltechnologie GmbH, Friedrich-Barnewitz-Straße 4, 18119 Rostock, Germany.
authors.forename: Cordula
authors.initials: C
authors.lastname: Grüttner
authors.affiliation: Fraunhofer Institute for Interfacial Engineering & Biotechnology IGB, Translational Center "Regenerative Therapies for Oncology & Musculoskeletal Diseases" - Würzburg branch, Röntgenring 11, 97070 Würzburg, Germany.|Tissue Engineering & Regenerative Medicine, University Hospital Würzburg, Röntgenring 11, 97070 Würzburg, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Steinke
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010316:Particle Size; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D058185:Magnetite Nanoparticles
keywords: metal nanoparticles; regenerative medicine; stem cells