title: The rs6983267 SNP and long non-coding RNA CARLo-5 are associated with endometrial carcinoma.
abstract: The single nucleotide polymorphism (SNP) rs6983267 and cancer-associated region long non-coding RNA (CARLo-5) are associated with various human cancers. This study aimed to investigate the expression of CARLo-5 in endometrial carcinoma (EC) and its relationship with clinicopathological features and patient survival. The association of the rs6983267 SNP with EC risk and its involvement in the regulation of CARLo-5 expression in EC were investigated. The rs6983267 SNP was genotyped by polymerase chain reaction (PCR) and ligase detection reaction in 543 EC patients and 584 controls. The expression of CARLo-5 in 108 EC tissues and 66 normal endometrial tissues (NETs) was determined using quantitative real-time PCR. The genotype and allele distributions of the rs6983267 SNP differed significantly between patients and controls. There was a significant correlation between the rs6983267 genotypes and lymph node metastasis of EC patients (P = 0.026). CARLo-5 expression was significantly higher in EC tissues than in NETs (P < 0.001) and significantly associated with FIGO stage (P = 0.029) and lymph node metastasis (P = 0.030). Patients with high CARLo-5 expression had significantly shorter overall survival than those with low CARLo-5 expression (P = 0.003). The rs6983267 genotype was significantly correlated with CARLo-5 expression (P < 0.05). In conclusion, CARLo-5 was identified as a pro-oncogenic lncRNA that may play an important role in EC progression and represent a prognostic marker for EC. The expression of CARLo-5 was significantly correlated with the rs6983267 genotype associated with increased susceptibility to EC. Environ. Mol. Mutagen. 57:508-515, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Obstetrics and Gynecology, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Xiwa
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics and Gynecology, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Xurui
authors.initials: X
authors.lastname: Wei
authors.affiliation: Tumor Research Institute, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Lianmei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics and Gynecology, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Shi
authors.affiliation: Department of Obstetrics and Gynecology, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Obstetrics and Gynecology, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Kang
authors.affiliation: Department of Obstetrics and Gynecology, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D004247:DNA / Q000235:genetics; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006801:Humans; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: C579842:CCAT1 long noncoding RNA, human; D062085:RNA, Long Noncoding; D004247:DNA
keywords: CARLo-5; endometrial carcinoma; prognosis; rs6983267; susceptibility