title: Variation in the Gut Microbiota of Termites (Tsaitermes ampliceps) Against Different Diets.
abstract: Termites are well recognized for their thriving on recalcitrant lignocellulosic diets through nutritional symbioses with gut-dwelling microbiota; however, the effects of diet changes on termite gut microbiota are poorly understood, especially for the lower termites. In this study, we employed high-throughput 454 pyrosequencing of 16S V1-V3 amplicons to compare gut microbiotas of Tsaitermes ampliceps fed with lignin-rich and lignin-poor cellulose diets after a 2-week-feeding period. As a result, the majority of bacterial taxa were shared across the treatments with different diets, but their relative abundances were modified. In particular, the relative abundance was reduced for Spirochaetes and it was increased for Proteobacteria and Bacteroides by feeding the lignin-poor diet. The evenness of gut microbiota exhibited a significant difference in response to the diet type (filter paper diets < corn stover diets < wood diets), while their richness was constant, which may be related to the lower recalcitrance of this biomass to degradation. These results have important implications for sampling and analysis strategies to probe the lignocellulose degradation features of termite gut microbiota and suggest that the dietary lignocellulose composition could cause shifting rapidly in the termite gut microbiota.
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Su
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
authors.forename: Lele
authors.initials: L
authors.lastname: Yang
authors.affiliation: BioEnergy Genome Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, 266101, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Huang
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: BioEnergy Genome Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, 266101, China.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Su
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan, 450002, China.|Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, Zhengzhou, Henan, 450002, China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Wang
authors.affiliation: BioEnergy Genome Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, 266101, China.
authors.forename: Cunpei
authors.initials: C
authors.lastname: Bo
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340, México D.F., Mexico. entaowang@yahoo.com.mx.
authors.forename: En Tao
authors.initials: ET
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan, 450002, China. songandong@henau.edu.cn.|Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, Zhengzhou, Henan, 450002, China. songandong@henau.edu.cn.|, No. 93, Nongye Road, Zhengzhou, Henan Province, China. songandong@henau.edu.cn.
authors.forename: Andong
authors.initials: A
authors.lastname: Song
mesh_terms: D000818:Animals; D001439:Bacteroides / Q000187:drug effects / Q000235:genetics; D004032:Diet; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D020049:Isoptera / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D008031:Lignin / Q000378:metabolism* / Q000494:pharmacology; D020560:Proteobacteria / Q000187:drug effects / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013144:Spirochaetales / Q000187:drug effects / Q000235:genetics; D013559:Symbiosis / Q000187:drug effects / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S; D008031:Lignin
keywords: 454 Pyrosequencing; Corn stover; Filter paper; Gut symbionts; Lignocellulose degradation; Tsaitermes ampliceps