title: miRNA signatures and transcriptional regulation of their target genes in vitiligo.
abstract: BACKGROUND
  miRNAs are small non-coding RNA molecules that post-transcriptionally regulate gene expression. We have earlier reported the skin miRNA expression profiling in patients with non-segmental vitiligo.
  
  
  OBJECTIVE
  In the present study, we show the expression of previously identified skin miRNAs signatures in blood and their target genes in whole blood and PBMCs as well as skin micro-environment of vitiligo patients and controls.
  
  
  METHODS
  miRNA expression profiling in whole blood was performed using customized TaqMan®Low Density Array. We predicted the potential targets of differentially expressed miRNAs and investigated their expression levels in skin, whole blood and PBMCs from patients and controls using Real-time PCR.
  
  
  RESULTS
  Our results showed miR-1, miR-184, miR-328, miR-383 and miR-577 hold similar pattern of expression as of skin, suggesting their potent eminence for being putative markers for vitiligo. In silico target prediction revealed miR-1 targets EDN1, G6PD, HSP60, HSP70, SERP1, SIRT1 & TYR; miR-184 targets EZR & LAMP1; miR-328 targets IL1B, POLH & TRPM1; miR-383 targets EDN1 & TYRP1; and miR-577 targets PTPN22 & TYRP1 which were corroborated by our validation study.
  
  
  CONCLUSIONS
  In conclusion, the present study for the first time provides new insights into the crucial role of miRNA regulated gene network involved in oxidative stress, autoimmunity and ER stress mediated pathogenesis of vitiligo.
authors.affiliation: Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390002, India.
authors.forename: Mohmmad Shoab
authors.initials: MS
authors.lastname: Mansuri
authors.affiliation: Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390002, India.
authors.forename: Mala
authors.initials: M
authors.lastname: Singh
authors.affiliation: Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390002, India. Electronic address: rasheedunnisab@yahoo.co.in.
authors.forename: Rasheedunnisa
authors.initials: R
authors.lastname: Begum
mesh_terms: D000328:Adult; D001769:Blood / Q000378:metabolism; D016022:Case-Control Studies; D059865:Endoplasmic Reticulum Stress; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000276:immunology / Q000378:metabolism; D014158:Transcription, Genetic; D014820:Vitiligo / Q000235:genetics* / Q000378:metabolism*; D055815:Young Adult
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Autoimmunity; Oxidative stress; Skin; Vitiligo; miRNA; miRNA target