title: Detection of Activation Antigens on Chronic Lymphocytic Leukaemia Cells.
abstract: The peripheral blood mononuclear cells of patients with chronic lymphocytic leukaemia were characterized by the presence of a variety of cell surface differentiation antigens. The cells of 20 patients were found to be of B-cell phenotype when studied with antibodies directed against CD19, CD20, HLA-DR and sIg. Furthermore, a significant percentage of the cells gave a positive reaction with the monoclonal antibody to CD5. On the other hand, the CLL-cells did not express the CD21 antigen (C3d receptor, EBV receptor). We studied in parallel the presence of various activation antigens using 19 monoclonal antibodies grouped into 7 clusters (CD25, CD30, CD40, CD69, CD70, CD39, CD71). A significantly higher percentage of the CLL cells expressed activation antigens than lymphocytes from healthy controls. The percentage of CD3/HLA + DR + cells, compared to the healthy control lymphocytes was not increased in the CLL patients, and the activated cells in CLL were found to have characteristics of B-cells. Based on these results, we suggest that the CLL cells, like the cells in Hodgkin's disease and T-cell lymphoma, are not resting, but activated B-cells or the neoplastic abberrants of activated cells.
authors.affiliation: a National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Pálóczi
authors.affiliation: a National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
authors.forename: É
authors.initials: É
authors.lastname: Pócsik
authors.affiliation: a National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
authors.forename: R
authors.initials: R
authors.lastname: Mihalik
authors.affiliation: a National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Benczur
authors.affiliation: a National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Demeter
authors.affiliation: a National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
authors.forename: V
authors.initials: V
authors.lastname: Solti
authors.affiliation: a National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Petrányi
authors.affiliation: a National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
authors.forename: S R
authors.initials: SR
authors.lastname: Hollan
keywords: Activation antigens; CLL