title: Microsome Isolation from Tissue.
abstract: This protocol details the extraction of microsomes from frozen tissue in order to further examine the protein-protein interactions occurring within the endoplasmic reticulum. This protocol was adapted from Abisambraet al.(2013) with modifications made in order to optimize for subsequent use.
authors.affiliation: Sanders-Brown Center on Aging and Department of Physiology, University of Kentucky, Lexington, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Bodero
authors.affiliation: Sanders-Brown Center on Aging and Department of Physiology, University of Kentucky, Lexington, USA.
authors.forename: Jose
authors.initials: J
authors.lastname: Francisco Abisambra