title: Bioconversion of L-phenylalanine to 2-phenylethanol by the novel stress-tolerant yeast Candida glycerinogenes WL2002-5.
abstract: 2-Phenylethanol (2-PE) is a high value aromatic alcohol with a rose-like odor that is utilized in the cosmetics and other industries. Although the chemical routes of 2-PE production have been altered by some microbial transformation processes, the poor tolerance to organic solvents of these microorganisms has limited the 2-PE yield. In this study, the stress-tolerant yeast Candida glycerinogenes WL2002-5 showed a 2-PE tolerance to 4 g/l, which is the highest reported to date. Moreover, the 2-PE titer in a batch fermentation from L-phenylalanine reached 5g/l, which is the highest level achieved by fermentation without in situ product recovery. These results suggest C. glycerinogenes WL2002-5 is a robust strain for the bioproduction of 2-PE with potential for commercial exploitation.
authors.affiliation: a The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.|b The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.
authors.forename: Xinyao
authors.initials: X
authors.lastname: Lu
authors.affiliation: a The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.|b The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.|b The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zong
authors.affiliation: a The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.|b The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.
authors.forename: Hao
authors.initials: H
authors.lastname: Ji
authors.affiliation: a The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.|b The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhuge
authors.affiliation: b The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.
authors.forename: Zhuoli
authors.initials: Z
authors.lastname: Dong
mesh_terms: D002175:Candida / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D010649:Phenylalanine / Q000378:metabolism*; D010626:Phenylethyl Alcohol / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D010649:Phenylalanine; D010626:Phenylethyl Alcohol
keywords: 2-phenylethanol; Candida glycerinogenes; L-phenylalanine; bioproduction; stress tolerance