title: Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis.
abstract: Rapid and quantitative sensing of aflatoxin B1 with high sensitivity and specificity has drawn increased attention of studies investigating soybean sauce. A sensitive and rapid quantitative immunochromatographic sensing method was developed for the detection of aflatoxin B1 based on time-resolved fluorescence. It combines the advantages of time-resolved fluorescent sensing and immunochromatography. The dynamic range of a competitive and portable immunoassay was 0.3-10.0 µg·kg(-1), with a limit of detection (LOD) of 0.1 µg·kg(-1) and recoveries of 87.2%-114.3%, within 10 min. The results showed good correlation (R² > 0.99) between time-resolved fluorescent immunochromatographic strip test and high performance liquid chromatography (HPLC). Soybean sauce samples analyzed using time-resolved fluorescent immunochromatographic strip test revealed that 64.2% of samples contained aflatoxin B1 at levels ranging from 0.31 to 12.5 µg·kg(-1). The strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique in food safety analysis.
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. wang416929@gmail.com.|Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China. wang416929@gmail.com.
authors.forename: Du
authors.initials: D
authors.lastname: Wang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. zwzhang@whu.edu.cn.|Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China. zwzhang@whu.edu.cn.
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. peiwuli@oilcrops.cn.|Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China. peiwuli@oilcrops.cn.|Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China. peiwuli@oilcrops.cn.|Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China. peiwuli@oilcrops.cn.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Li
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. zhangqi01@caas.cn.|Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China. zhangqi01@caas.cn.|Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China. zhangqi01@caas.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. zhangwen@oilcrops.cn.|Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China. zhangwen@oilcrops.cn.|Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China. zhangwen@oilcrops.cn.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D059022:Food Safety; D057230:Limit of Detection; D045730:Soy Foods / Q000032:analysis*
chemical_list: D016604:Aflatoxin B1
keywords: aflatoxin B1; food safety; soybean sauce; time-resolved fluorescent immunochromatographic strip