title: A Functional rs353293 Polymorphism in the Promoter of miR-143/145 Is Associated with a Reduced Risk of Bladder Cancer.
abstract: OBJECTIVE
  MicroRNA (miR)-143/145, known as tumor suppressors, can promote cell apoptosis and differentiation, and suppress cell proliferation, invasion and migration. We performed a case-control study to investigate the association of rs353293 in the promoter region of miR-143/145 with bladder cancer risk.
  
  
  METHODS
  In total, 869 subjects including 333 cases and 536 controls were enrolled in this study, and the rs353293 polymorphism was genotyped by using a Taqman assay. The promoter activity was measured by the Dual-Luciferase Assay System.
  
  
  RESULTS
  We calculated an adjusted odds ratio of 0.64 for the presence of either AA/AG genotypes (95% CI 0.46-0.90) and 0.64 (95% CI 0.47-0.87) for carrying at least one A allele in bladder cancer. Stratified analyses showed that the AA/AG genotypes and the A allele were less prevalent in patients with low grade tumors, compared to those harboring higher grade bladder cancers (adjusted OR = 0.53, 95% CI, 0.30-0.94, P = 0.03 and adjusted OR = 0.54, 95% CI, 0.32-0.92, P = 0.02, respectively). In vitro luciferase reporter analysis showed that rs353293A allele had a lower activity compared with the rs353293G allele (P < 0.001).
  
  
  CONCLUSIONS
  These findings suggest that the functional rs353293 polymorphism may be a useful biomarker to predict the risk of bladder cancer.
authors.affiliation: Department of Urinary Surgery, Affiliated Hospital of Youjiang Medical College for Nationalities, Baise, 533000, Guangxi, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Urinary Surgery, Affiliated Hospital of Youjiang Medical College for Nationalities, Baise, 533000, Guangxi, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Urinary Surgery, Affiliated Hospital of Youjiang Medical College for Nationalities, Baise, 533000, Guangxi, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Meng
authors.affiliation: Department of Urinary Surgery, Affiliated Hospital of Youjiang Medical College for Nationalities, Baise, 533000, Guangxi, China.
authors.forename: Minyu
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Urinary Surgery, Affiliated Hospital of Youjiang Medical College for Nationalities, Baise, 533000, Guangxi, China.
authors.forename: Chaowen
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Urinary Surgery, Affiliated Hospital of Youjiang Medical College for Nationalities, Baise, 533000, Guangxi, China.
authors.forename: Tianzi
authors.initials: T
authors.lastname: Qin
mesh_terms: D045744:Cell Line, Tumor; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D014158:Transcription, Genetic; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
chemical_list: C542359:MIRN143 microRNA, human; C541010:MIRN145 microRNA, human; D035683:MicroRNAs