title: Association between tuberculosis and circulating microRNA hsa-let-7b and hsa-miR-30b: A pilot study in a Chinese population.
abstract: Tuberculosis remains one of the world's deadliest communicable diseases. Limitations in the current diagnosis tools have heavily slowed down the step to eliminate TB. The objective of this study was to identify potential circulating miRNA associated with tuberculosis (TB) infection and disease development. Agilent human miRNA microarray was used to estimate the circulating levels of 1887 miRNAs among 34 study participants (10 patients with pulmonary TB, 13 controls with latent TB infection and 11 non-infected healthy controls). The identified miRNAs were subsequently verified by real-time qPCR. Target gene prediction and miRNA-gene network construction were further explored. A total of 119 miRNAs were identified to be in different levels between any two groups of the study population by microarray (Fold Change>2, p < 0.01). 11 most promising miRNAs were then selected for verification by real-time qPCR. The levels of hsa-let-7b-5p and hsa-miR-30b-5p were confirmed to be significantly up-regulated in pulmonary TB patients as compared to both control groups (p < 0.01). Caspase 3 was predicted to be one common target gene for these two miRNAs. None of the selected miRNA was confirmed to be related with the infection status. This pilot study suggested circulating hsa-let-7b and hsa-miR-30b might be associated with TB development by regulating the target genes involved in TLR-NF-kB mediated signal pathway.
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Henan
authors.initials: H
authors.lastname: Xin
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Peking University Health Science Centre, Beijing 100191, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: The Sixth People's Hospital of Zhengzhou, Zhengzhou 450063, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Liu
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Li
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Mufei
authors.initials: M
authors.lastname: Li
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Boxuan
authors.initials: B
authors.lastname: Feng
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Haoran
authors.initials: H
authors.lastname: Zhang
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Hengjing
authors.initials: H
authors.lastname: Li
authors.affiliation: The Sixth People's Hospital of Zhengzhou, Zhengzhou 450063, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shen
authors.affiliation: The Sixth People's Hospital of Zhengzhou, Zhengzhou 450063, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Guan
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. Electronic address: gaolei@ipbcams.ac.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002681:China; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D056726:Genetic Association Studies; D005819:Genetic Markers; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010865:Pilot Projects; D060888:Real-Time Polymerase Chain Reaction; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D005819:Genetic Markers; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human
keywords: Circulating microRNA; Latent tuberculosis infection; Pulmonary tuberculosis; hsa-let-7b; hsa-miR-30b