title: Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Can Precisely Discriminate the Lineages of Listeria monocytogenes and Species of Listeria.
abstract: The genetic lineages of Listeria monocytogenes and other species of the genus Listeria are correlated with pathogenesis in humans. Although matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has become a prevailing tool for rapid and reliable microbial identification, the precise discrimination of Listeria species and lineages remains a crucial issue in clinical settings and for food safety. In this study, we constructed an accurate and reliable MS database to discriminate the lineages of L. monocytogenes and the species of Listeria (L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii, L. grayi, and L. rocourtiae) based on the S10-spc-alpha operon gene encoded ribosomal protein mass spectrum (S10-GERMS) proteotyping method, which relies on both genetic information (genomics) and observed MS peaks in MALDI-TOF MS (proteomics). The specific set of eight biomarkers (ribosomal proteins L24, L6, L18, L15, S11, S9, L31 type B, and S16) yielded characteristic MS patterns for the lineages of L. monocytogenes and the different species of Listeria, and led to the construction of a MS database that was successful in discriminating between these organisms in MALDI-TOF MS fingerprinting analysis followed by advanced proteotyping software Strain Solution analysis. We also confirmed the constructed database on the proteotyping software Strain Solution by using 23 Listeria strains collected from natural sources.
authors.affiliation: Knowledge Hub Aichi, Aichi Science and Technology Foundation, Yakusa, Toyota, Aichi 470-0356, Japan.|School of Agriculture, Meijo University, Shiogamaguchi, Tenpaku-ku, Nagoya, Aichi 468-0073, Japan.
authors.forename: Teruyo
authors.identifier: http://orcid.org/0000-0001-5290-5176
authors.initials: T
authors.lastname: Ojima-Kato
authors.affiliation: School of Agriculture, Meijo University, Shiogamaguchi, Tenpaku-ku, Nagoya, Aichi 468-0073, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo, 108-8477 Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Knowledge Hub Aichi, Aichi Science and Technology Foundation, Yakusa, Toyota, Aichi 470-0356, Japan.|School of Agriculture, Meijo University, Shiogamaguchi, Tenpaku-ku, Nagoya, Aichi 468-0073, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Tamura
mesh_terms: D019295:Computational Biology / Q000379:methods; D016172:DNA Fingerprinting / Q000379:methods; D030541:Databases, Genetic; D008087:Listeria / Q000145:classification* / Q000235:genetics; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods