title: Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells.
abstract: Research into adipose tissue-derived mesenchymal stem cells (AD-MSCs) has demonstrated the feasibility of their use in clinical applications due to their ease of isolation and abundance in adipose tissue. We isolated AD-MSCs from young and old dogs, and the cells were subjected to sequential sub-passaging from passage 1 (P1) to P7. Canine AD-MSCs (cAD-MSCs) were examined for proliferation kinetics, expression of molecules associated with self-renewal, expression of cell surface markers, and differentiation potentials at P3. Cumulative population doubling level was significantly higher in cAD-MSCs of young donors than in those of old donors. In addition, expressions of CD73, CD80, Oct3/4, Nanog, cell survival genes and differentiation potentials were significantly higher in young donors than in old donors. The present study suggests that donor age should be considered when developing cell-based therapies for clinical application of cAD-MSCs.
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: Jienny
authors.initials: J
authors.lastname: Lee
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: Keum Sil
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: Chan-Lan
authors.initials: CL
authors.lastname: Kim
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: Jeong Su
authors.initials: JS
authors.lastname: Byeon
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: Na-Yeon
authors.initials: NY
authors.lastname: Gu
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Cho
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Cha
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D000367:Age Factors; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology*; D004285:Dogs; D020869:Gene Expression Profiling / Q000662:veterinary; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
keywords: adipose mesenchymal stem cells; age; canine; differentiation; multipotency