title: Review of Beet pseudoyellows virus genome structure built the consensus genome organization of cucumber strains and highlighted the unique feature of strawberry strain.
abstract: The complete nucleotide sequences of Beet pseudoyellows virus (BPYV)-MI (cucumber isolate; Matsuyama, Idai) genomic RNAs 1 and 2 were determined and compared with the previously sequenced Japanese cucumber strain (BPYV-JC) and a strawberry strain (BPYV-S). The RNA 2 of BPYV-MI showed 99 % nucleotide sequence identity with both BPYV-JC and -S having highly conserved eight ORFs. In contrast, the RNA1 of BPYV-MI showed sequence identities of 98 and 86 % with BPYV-JC and -S, respectively. Phylogenetic analysis of RNA-dependent RNA polymerase (RdRp) coding sequences from three fully sequenced BPYV strains and five partially sequenced cucurbit-infecting BPYV strains from Japan and South Africa has shown that cucurbit-infecting strains are closer to each other than to BPYV-S. In addition, the strawberry strain BPYV-S has an ORF2 in the downstream of RdRp gene in RNA1, but all the cucumber strains, BPYV-JC, -MI, and those from South Africa, lacked the ORF2 of RNA1, highlighting the difference between common BPYV cucumber strains and a unique strawberry strain.
authors.affiliation: Laboratory of Plant Molecular Biology and Virology, Faculty of Agriculture, The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan.|Fruit Research Station, Bangladesh Agricultural Research Institute (BARI), Rajshahi, 6206, Bangladesh.
authors.forename: Md Shamim
authors.initials: MS
authors.lastname: Akhter
authors.affiliation: Laboratory of Plant Molecular Biology and Virology, Faculty of Agriculture, The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan.
authors.forename: Sachin Ashok
authors.initials: SA
authors.lastname: Bhor
authors.affiliation: Department of Plant Pathology, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville, Pietermaritzburg, 3209, South Africa.
authors.forename: Ngcebo
authors.initials: N
authors.lastname: Hlalele
authors.affiliation: Ehime Prefecture Plant Protection Office, Matsuyama, Ehime, 790-2405, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nao
authors.affiliation: Iwate Biotechnology Research Center, Kitakami, Iwate, 024-0003, Japan.|Faculty of Agriculture, University of the Ryukyus, Nakagami, Okinawa, 903-0213, Japan.
authors.forename: Ken-Taro
authors.initials: KT
authors.lastname: Sekine
authors.affiliation: Laboratory of Plant Molecular Biology and Virology, Faculty of Agriculture, The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yaeno
authors.affiliation: Laboratory of Plant Molecular Biology and Virology, Faculty of Agriculture, The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Yamaoka
authors.affiliation: Laboratory of Plant Molecular Biology and Virology, Faculty of Agriculture, The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nishiguchi
authors.affiliation: Department of Plant Pathology, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville, Pietermaritzburg, 3209, South Africa.
authors.forename: Augustine
authors.initials: A
authors.lastname: Gubba
authors.affiliation: Laboratory of Plant Molecular Biology and Virology, Faculty of Agriculture, The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan. kappei@ehime-u.ac.jp.
authors.forename: Kappei
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D029383:Crinivirus / Q000145:classification* / Q000235:genetics*; D018553:Cucumis sativus / Q000821:virology*; D031985:Fragaria / Q000821:virology*; D016679:Genome, Viral; D023281:Genomics; D054884:Host-Pathogen Interactions; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D017423:Sequence Analysis, RNA
keywords: BPYV; Crinivirus; Cucumber; Host adaptation; Strawberry