title: Chemical Profiling of the Essential Oils of Syzygium aqueum, Syzygium samarangense and Eugenia uniflora and Their Discrimination Using Chemometric Analysis.
abstract: The essential oil compositions of the leaves of three related Myrtaceae species, namely Syzygium aqueum, Syzygium samarangense and Eugenia uniflora, were investigated using GLC/MS and GLC/FID. Altogether, 125 compounds were identified: α-Selinene (13.85%), β-caryophyllene (12.72%) and β-selinene constitute the most abundant constituents in S. aqueum. Germacrene D (21.62%) represents the major compound in S. samarangense whereas in E. uniflora, spathulenol (15.80%) represents the predominant component. Multivariate chemometric analyses were used to discriminate the essential oils using hierarchical cluster analysis (HCA) and principal component analysis (PCA) based on the chromatographic results. The antimicrobial activity of the popularly used E. uniflora essential oil was assessed using broth microdilution method against six Gram-positive, three Gram-negative bacteria and two fungi. The oil showed moderate antimicrobial activity against Bacillus licheniformis exhibiting MIC and MMC of 0.63 mg/ml. The cytotoxic activity of E. uniflora essential oil was investigated against Trypanosoma brucei brucei (T. b. brucei) and MCF-7 cancer cell line using MTT assay. It showed moderate activity against MCF-7 cells with an IC50value of 76.40 μg/ml. On the other hand, T. brucei was highly susceptible to E. uniflora essential oil with IC50of 11.20 μg/ml, and a selectivity index of 6.82.
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, DE-69120, Heidelberg, Germany.
authors.forename: Mansour
authors.initials: M
authors.lastname: Sobeh
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, DE-69120, Heidelberg, Germany.
authors.forename: Markus Santhosh
authors.initials: MS
authors.lastname: Braun
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, DE-69120, Heidelberg, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Krstin
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University Abbassia, 11566, Cairo, Egypt.
authors.forename: Fadia S
authors.initials: FS
authors.lastname: Youssef
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University Abbassia, 11566, Cairo, Egypt.
authors.forename: Mohamed L
authors.initials: ML
authors.lastname: Ashour
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, DE-69120, Heidelberg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Wink
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D000935:Antifungal Agents / Q000032:analysis / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000494:pharmacology*; D000981:Antiprotozoal Agents / Q000032:analysis / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D016000:Cluster Analysis; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D000068856:Eugenia / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000032:analysis / Q000494:pharmacology*; D021261:Parasitic Sensitivity Tests; D018515:Plant Leaves / Q000737:chemistry; D025341:Principal Component Analysis; D013329:Structure-Activity Relationship; D027842:Syzygium / Q000737:chemistry*; D014346:Trypanosoma brucei brucei / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D000981:Antiprotozoal Agents; D009822:Oils, Volatile
keywords: Biological activity; Chemical composition; Multivariate chemometric analysis; Myrtaceae; Volatile oils