title: Differential Effects of Viscum album Preparations on the Maturation and Activation of Human Dendritic Cells and CD4⁺ T Cell Responses.
abstract: Extracts of Viscum album (VA); a semi-parasitic plant, are frequently used in the complementary therapy of cancer and other immunological disorders. Various reports show that VA modulates immune system and exerts immune-adjuvant activities that might influence tumor regression. Currently, several therapeutic preparations of VA are available and hence an insight into the mechanisms of action of different VA preparations is necessary. In the present study, we performed a comparative study of five different preparations of VA on maturation and activation of human dendritic cells (DCs) and ensuing CD4⁺ T cell responses. Monocyte-derived human DCs were treated with VA Qu Spez, VA Qu Frf, VA M Spez, VA P and VA A. Among the five VA preparations tested VA Qu Spez, a fermented extract with a high level of lectins, significantly induced DC maturation markers CD83, CD40, HLA-DR and CD86, and secretion of pro-inflammatory cytokines such as IL-6, IL-8, IL-12 and TNF-α. Furthermore, analysis of T cell cytokines in DC-T cell co-culture revealed that VA Qu Spez significantly stimulated IFN-γ secretion without modulating regulatory T cells and other CD4⁺ T cytokines IL-4, IL-13 and IL-17A. Our study thus delineates differential effects of VA preparations on DC maturation; function and T cell responses.
authors.affiliation: Institut National de la Sant&#233; et de la Recherche M&#233;dicale Unit&#233; 1138, Centre de Recherche des Cordeliers, 15 rue de l'Ecole de M&#233;dicine, Paris F-75006, France. chaitrali.roy@gmail.com.|Universit&#233; de Technologie de Compi&#232;gne, UMR CNRS 6022, Compi&#232;gne F-60205, France. chaitrali.roy@gmail.com.|Centre de Recherche des Cordeliers, Immunopathologie et Immuno-Intervention Th&#233;rapeutique, Paris F-75006, France. chaitrali.roy@gmail.com.
authors.forename: Chaitrali
authors.initials: C
authors.lastname: Saha
authors.affiliation: Institut National de la Sant&#233; et de la Recherche M&#233;dicale Unit&#233; 1138, Centre de Recherche des Cordeliers, 15 rue de l'Ecole de M&#233;dicine, Paris F-75006, France. mdasmicro@gmail.com.|Centre de Recherche des Cordeliers, Immunopathologie et Immuno-Intervention Th&#233;rapeutique, Paris F-75006, France. mdasmicro@gmail.com.|Sorbonne Universit&#233;s, UPMC Univ Paris 06, UMR S 1138, Paris F-75006, France. mdasmicro@gmail.com.
authors.forename: Mrinmoy
authors.initials: M
authors.lastname: Das
authors.affiliation: Institut National de la Sant&#233; et de la Recherche M&#233;dicale Unit&#233; 1138, Centre de Recherche des Cordeliers, 15 rue de l'Ecole de M&#233;dicine, Paris F-75006, France. esvkai@gmail.com.|Centre de Recherche des Cordeliers, Immunopathologie et Immuno-Intervention Th&#233;rapeutique, Paris F-75006, France. esvkai@gmail.com.|Sorbonne Universit&#233;s, UPMC Univ Paris 06, UMR S 1138, Paris F-75006, France. esvkai@gmail.com.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Stephen-Victor
authors.affiliation: Universit&#233; de Technologie de Compi&#232;gne, UMR CNRS 6022, Compi&#232;gne F-60205, France. alain.friboulet@utc.fr.
authors.forename: Alain
authors.initials: A
authors.lastname: Friboulet
authors.affiliation: Institut National de la Sant&#233; et de la Recherche M&#233;dicale Unit&#233; 1138, Centre de Recherche des Cordeliers, 15 rue de l'Ecole de M&#233;dicine, Paris F-75006, France. jagadeesh.bayry@crc.jussieu.fr.|Centre de Recherche des Cordeliers, Immunopathologie et Immuno-Intervention Th&#233;rapeutique, Paris F-75006, France. jagadeesh.bayry@crc.jussieu.fr.|Sorbonne Universit&#233;s, UPMC Univ Paris 06, UMR S 1138, Paris F-75006, France. jagadeesh.bayry@crc.jussieu.fr.|Universit&#233; Paris Descartes, Sorbonne Paris Cit&#233;, UMR S 1138, Paris F-75006, France. jagadeesh.bayry@crc.jussieu.fr.
authors.forename: Jagadeesh
authors.initials: J
authors.lastname: Bayry
authors.affiliation: Institut National de la Sant&#233; et de la Recherche M&#233;dicale Unit&#233; 1138, Centre de Recherche des Cordeliers, 15 rue de l'Ecole de M&#233;dicine, Paris F-75006, France. srini.kaveri@crc.jussieu.fr.|Centre de Recherche des Cordeliers, Immunopathologie et Immuno-Intervention Th&#233;rapeutique, Paris F-75006, France. srini.kaveri@crc.jussieu.fr.|Sorbonne Universit&#233;s, UPMC Univ Paris 06, UMR S 1138, Paris F-75006, France. srini.kaveri@crc.jussieu.fr.|Universit&#233; Paris Descartes, Sorbonne Paris Cit&#233;, UMR S 1138, Paris F-75006, France. srini.kaveri@crc.jussieu.fr.
authors.forename: Srini V
authors.initials: SV
authors.lastname: Kaveri
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D016131:Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D028182:Viscum album / Q000737:chemistry*
chemical_list: D016207:Cytokines; D010936:Plant Extracts
keywords: IFN-γ; T cell response; Th1; Th17; Th2; Viscum album; cytokines; dendritic cells; innate cells; maturation; regulatory T cell