title: The present and future of genome editing in cancer research.
abstract: The widespread use of high-throughput genome sequencing methods is profoundly changing the way we understand, classify, and treat human cancers. To make sense of the deluge of sequencing data generated in the clinic, more effective and rapid assessments of the functional relevance of newly discovered cancer-associated mutations are urgently needed. In this review, we discuss how genome editing technologies are responding to this major challenge. Largely focusing on CRISPR-based methods, we will highlight their potential to accelerate discovery, discuss their current limitations, and speculate about future applications.
authors.affiliation: Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.|Gerstner Sloan Kettering Graduate School of Biomedical Sciences, New York, NY, USA.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Li
authors.affiliation: Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.|Gerstner Sloan Kettering Graduate School of Biomedical Sciences, New York, NY, USA.
authors.forename: Raymond
authors.initials: R
authors.lastname: Wu
authors.affiliation: Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA. venturaa@mskcc.org.
authors.forename: Andrea
authors.identifier: 0000-0003-4320-9907
authors.initials: A
authors.lastname: Ventura
mesh_terms: D035843:Biomedical Research; D000072669:Gene Editing; D006801:Humans; D009369:Neoplasms / Q000235:genetics*