title: Fractionation of inorganic arsenic by adjusting hydrogen ion concentration.
abstract: The inorganic fraction of arsenic species, iAs=∑[As(III)+As(V)] present in fish samples can be quantified in the presence of other arsenic species also found in fishes, such as: monomethylarsonic acid (MMA), dimethylarsinic acid (DMA) and arsenobetaine (AsB). The toxic arsenic fraction was selected taking into account the dissociation constants of these arsenic species in different hydrogen ions concentration leading to the arsine formation from iAs compounds detected as As(III) by HG AAS. For thus, a microwave assisted extraction was carried out using HCl 1molL(-1) in order to maintain the integrity of the arsenic species in this mild extraction media. Recovery experiments were done for iAs fraction, in the presence of other arsenic species. The recovery values obtained for iAs fraction added were quantitative about 87-107% (for N=3, RSD⩽3%). The limit of detection (LOD), and the limit of quantification (LOQ), were 5μgkg(-1) and 16μgkg(-1) respectively.
authors.affiliation: Federal University of Paraná - Chemistry Department, P.O. Box 19032, 81531-980 Curitiba, PR, Brazil. Electronic address: aoliveira@quimica.ufpr.br.
authors.forename: Andrea
authors.initials: A
authors.lastname: Oliveira
authors.affiliation: São Paulo State University (UNESP), Department of Chemistry and Environmental Science, P.O. Box 136, 15054-000 São José do Rio Preto, SP, Brazil.
authors.forename: Mario Henrique
authors.initials: MH
authors.lastname: Gonzalez
authors.affiliation: Ministry of Agriculture, Livestock and Food Supply (MAPA) - LANAGRO/SP, Brazil.
authors.forename: Helena Müller
authors.initials: HM
authors.lastname: Queiroz
authors.affiliation: Institute of Chemistry, Campinas State University, P.O. Box 6154, 13083-970 Campinas, SP, Brazil.
authors.forename: Solange
authors.initials: S
authors.lastname: Cadore
mesh_terms: D000818:Animals; D001151:Arsenic / Q000737:chemistry*; D001152:Arsenicals / Q000737:chemistry*; D005591:Chemical Fractionation; D002845:Chromatography; D004284:Dogfish; D005399:Fishes; D059022:Food Safety / Q000379:methods; D006859:Hydrogen / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008872:Microwaves; D009132:Muscles / Q000378:metabolism; D018570:Risk Assessment
chemical_list: D001152:Arsenicals; D006859:Hydrogen; C020300:monomethylarsonic acid; D001151:Arsenic; C038992:arsenobetaine; C006633:arsine
keywords: Fish samples; Hydride generation; Inorganic arsenic; Non-chromatographic separation