title: Stage-specific embryonic antigen: determining expression in canine glioblastoma, melanoma, and mammary cancer cells.
abstract: The expression of stage-specific embryonic antigens (SSEAs) was determined in several types of canine cancer cells. Flow cytometry showed SSEA-1 expression in glioblastoma, melanoma, and mammary cancer cells, although none expressed SSEA-3 or SSEA-4. Expression of SSEA-1 was not detected in lymphoma, osteosarcoma, or hemangiosarcoma cell lines. Relatively stable SSEA-1 expression was observed between 24 and 72 h of culture. After 8 days in culture, sorted SSEA-1-and SSEA-1+cells re-established SSEA-1 expression to levels comparable to those observed in unsorted cells. Our results document, for the first time, the expression of SSEA-1 in several canine cancer cell lines.
authors.affiliation: Department of Veterinary Clinical Sciences and Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.|Department of Veterinary Medicine, College of Life Sciences, Longyan University, Longyan 364012, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Lin
authors.affiliation: Department of Veterinary Clinical Sciences and Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Jaime F
authors.initials: JF
authors.lastname: Modiano
authors.affiliation: Department of Veterinary Clinical Sciences and Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D004283:Dog Diseases; D004285:Dogs; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D005909:Glioblastoma / Q000209:etiology / Q000378:metabolism / Q000662:veterinary*; D015674:Mammary Neoplasms, Animal / Q000209:etiology / Q000378:metabolism*; D008545:Melanoma / Q000209:etiology / Q000378:metabolism / Q000662:veterinary*; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism*
chemical_list: D055746:Stage-Specific Embryonic Antigens
keywords: canine cancer; flow cytometry; magnetic-activated cell sorting; stage-specific embryonic antigens