title: Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar 'Mizuhochikara'.
abstract: Rice bran oil is a byproduct of the milling of rice (Oryza sativa L.). It offers various health benefits and has a beneficial fatty acid composition. To increase the amount of rice bran as a sink for triacylglycerol (TAG), we developed and characterized new breeding materials with giant embryos. To induce mutants, we treated fertilized egg cells of the high-yielding cultivar 'Mizuhochikara' with N-methyl-N-nitrosourea (MNU). By screening M2 seeds, we isolated four giant embryo mutant lines. Genetic analysis revealed that the causative loci in lines MGE12 and MGE13 were allelic to giant embryo (ge) on chromosome 7, and had base changes in the causal gene Os07g0603700. On the other hand, the causative loci in lines MGE8 and MGE14 were not allelic to ge, and both were newly mapped on chromosome 3. The TAG contents of all four mutant lines increased relative to their wild type, 'Mizuhochikara'. MGE13 was agronomically similar to 'Mizuhochikara' and would be useful for breeding for improved oil content.
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Mitsukazu
authors.initials: M
authors.lastname: Sakata
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Seno
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Matsusaka
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Yamagata
authors.affiliation: Institute of Tropical Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Enrique R
authors.initials: ER
authors.lastname: Angeles
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Mochizuki
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kumamaru
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Sato
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Enomoto
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tashiro
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kuhara
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Satoh
authors.affiliation: Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yoshimura
keywords: N-methyl-N-nitrosourea (MNU); giant embryo; mutant; rice bran oil; triacylglycerol (TAG)