title: Lysine Acetylation and Succinylation in HeLa Cells and their Essential Roles in Response to UV-induced Stress.
abstract: Lysine acetylation and succinylation are major types of protein acylation that are important in many cellular processes including gene transcription, cellular metabolism, DNA damage response. Malfunctions in these post-translational modifications are associated with genome instability and disease in higher organisms. In this study, we used high-resolution nano liquid chromatography-tandem mass spectrometry combined with affinity purification to quantify the dynamic changes of protein acetylation and succinylation in response to ultraviolet (UV)-induced cell stress. A total of 3345 acetylation sites in 1440 proteins and 567 succinylation sites in 246 proteins were identified, many of which have not been reported previously. Bioinformatics analysis revealed that these proteins are involved in many important biological processes, including cell signalling transduction, protein localization and cell metabolism. Crosstalk analysis between these two modifications indicated that modification switches might regulate protein function in response to UV-induced DNA damage. We further illustrated that FEN1 acetylation at different sites could lead to different cellular phenotypes, suggesting the multiple function involvement of FEN1 acetylation under DNA damage stress. These systematic analyses provided valuable resources and new insight into the potential role of lysine acetylation and succinylation under physiological and pathological conditions.
authors.affiliation: Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Xuanyi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xu
authors.affiliation: Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Rongyi
authors.initials: R
authors.lastname: Shi
authors.affiliation: Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Suo
authors.affiliation: Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Kaiying
authors.initials: K
authors.lastname: Cheng
authors.affiliation: Institute of Zhejiang Cancer Research, Zhejiang Cancer Hospital, Hangzhou, 310022, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Zhejiang Institute of Microbiology, Hangzhou, 310000, China.
authors.forename: Meixia
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Liangyan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Tian
authors.affiliation: Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Yuejin
authors.initials: Y
authors.lastname: Hua
mesh_terms: D000107:Acetylation / Q000528:radiation effects*; D045744:Cell Line, Tumor; D004249:DNA Damage / Q000528:radiation effects; D042822:Genomic Instability / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D008239:Lysine / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000528:radiation effects; D013312:Stress, Physiological / Q000528:radiation effects*; D019802:Succinic Acid / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D019802:Succinic Acid; D008239:Lysine