title: Quadrupole time-of-flight mass spectrometry analysis of glycerophospholipid molecular species in the two halophyte seed oils: Eryngium maritimum and Cakile maritima.
abstract: Future applications of lipids in clinical cohort studies demand detailed glycerophospholipid molecule information and the application of high-throughput lipidomics platforms. In the present work, a novel sensitive technique with high mass resolution and accuracy was applied to accomplish phospholipid analysis. Nanospray ionization quadrupole time-of-flight mass spectrometry was used to separate and quantify the glycerophospholipid classes as well as molecular species in two halophyte seed oils from Cakile maritima and Eryngium maritimum. Precursor or neutral loss scans of their polar head groups allowed the detection of molecular species within particular glycerophospholipid classes. Phosphatidylcholine was found to be the most abundant glycerophospholipid in both seed oils whereas phosphatidylethanolamine and phosphatidic acid were less abundant. Phosphatidylinositol, phosphatidylserine and phosphatidylglycerol were minor glycerophospholipids. Several molecular species within each class were detected and the main molecular species (C36:4, C36:3, C36:2, 34:2 and C34:1) were quantitatively different between the two halophytes and the different glycerophospholipids.
authors.affiliation: Laboratory of Extremophile Plants, Center of Biotechnology of Borj-Cedria, P. O. Box 901, 2050 Hammam-Lif, Tunisia. Electronic address: manel.zitouni@yahoo.fr.
authors.forename: Manel
authors.initials: M
authors.lastname: Zitouni
authors.affiliation: Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany. Electronic address: vera.wewer@uni-bonn.de.
authors.forename: Vera
authors.initials: V
authors.lastname: Wewer
authors.affiliation: Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany. Electronic address: doermann@uni-bonn.de.
authors.forename: Peter
authors.initials: P
authors.lastname: Dörmann
authors.affiliation: Laboratory of Extremophile Plants, Center of Biotechnology of Borj-Cedria, P. O. Box 901, 2050 Hammam-Lif, Tunisia. Electronic address: chedly.abdelly@cbbc.rnrt.tn.
authors.forename: Chedly
authors.initials: C
authors.lastname: Abdelly
authors.affiliation: Laboratory of Biotechnology of Olive Tree, Center of Biotechnology of Borj-Cedria, P. O. Box 901, 2050 Hammam-Lif, Tunisia. Electronic address: nabilbtn@gmail.com.
authors.forename: Nabil
authors.initials: N
authors.lastname: Ben Youssef
mesh_terms: D031049:Eryngium / Q000378:metabolism*; D020404:Glycerophospholipids / Q000378:metabolism*; D013058:Mass Spectrometry; D010713:Phosphatidylcholines / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism*; D055051:Salt-Tolerant Plants / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism
chemical_list: D020404:Glycerophospholipids; D010713:Phosphatidylcholines; D010938:Plant Oils
keywords: Alpha-linolenic acid (pubChem CID: 5280934); Arachidic acid (pub Chem CID: 10467); Cakile maritima; Eicosadienoic acid (pubChem CID: 6439848); Erucic acid (pubChem CID: 5281116); Eryngium maritimum; Fatty acids; Glycerophospholipids; Gondoic acid (pubChem CID: 5282768); Linoleic acid (pub Chem CID: 5280450); Molecular species; Oleic acid (pubChem CID: 445639); Palmitic acid (pubChem CID: 985); Palmitoleic acid (pubChem CID: 445638); Q-TOF MS/MS; Stearic acid (pubChem CID: 5281)