title: Antimicrobial susceptibility profiles of Helicobacter pylori isolated from patients in North India.
abstract: Helicobacter pylori-related gastroduodenal diseases are very common in India. Antibiotic resistance to commonly used antibiotics against H. pylori is increasing very rapidly. The aim of this study was to determine the antimicrobial susceptibility patterns of H. pylori strains from India against commonly used antibiotics in H. pylori treatment. Helicobacter pylori were cultured from 68 patients suffering from various gastroduodenal diseases in North India. Minimum inhibitory concentrations (MICs) to different antibiotics were determined by agar dilution. The clinical diagnosis of the 68 patients who were H. pylori culture-positive were gastro-oesophageal reflux disease (GERD) (n=23), non-erosive reflux disease (NERD) (n=22), non-ulcer dyspepsia (NUD) (n=13), antral gastritis (n=3), duodenal ulcer (n=2) and others (n=5). Of the 68 H. pylori isolates, 20 (29.4%) showed no resistance. The prevalence of drug resistance was 70.6%, including resistance to metronidazole (48.5%), furazolidone (22.1%), amoxicillin (17.6%), tetracycline (16.2%) and clarithromycin (11.8%). Dual and multiple drug resistance were found in 26.5% and 8.8% of cases, respectively. In conclusion, more than two-thirds of the isolated H. pylori strains showed resistance to at least one of the antibiotics for H. pylori treatment. Metronidazole resistance was most prevalent amongst the isolates tested. Emergence of dual and multidrug resistance is of great concern and there is an urgent need for regular antibiotic resistance surveillance studies. Amoxicillin- and clarithromycin-based anti-H. pylori regimens commonly prescribed for triple therapy in India show least resistance and hence are appropriate for anti-H. pylori management in India.
authors.affiliation: Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.
authors.forename: Valentina
authors.initials: V
authors.lastname: Gehlot
authors.affiliation: Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Mahant
authors.affiliation: National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India.
authors.forename: Asish Kumar
authors.initials: AK
authors.lastname: Mukhopadhyay
authors.affiliation: Yashoda Superspeciality Hospital, Ghaziabad, Uttar Pradesh, India; Yatharth Wellness Superspeciality Hospital, Noida, Uttar Pradesh, India.
authors.forename: Kunal
authors.initials: K
authors.lastname: Das
authors.affiliation: National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India.
authors.forename: Ronita
authors.initials: R
authors.lastname: De
authors.affiliation: Maulana Azad Medical College and L.N. Hospital, New Delhi, India.
authors.forename: Premashis
authors.initials: P
authors.lastname: Kar
authors.affiliation: Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India. Electronic address: rajashreepatra79@yahoo.co.in.
authors.forename: Rajashree
authors.initials: R
authors.lastname: Das
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D007194:India; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiotics; Helicobacter pylori; Multidrug resistance