title: miR-148b-3p inhibits malignant biological behaviors of human glioma cells induced by highHOTAIRexpression.
abstract: Increasing evidence suggests that long non coding (lnc)RNA and microRNA (miRNA/miR) both regulate the expression of key genes in tumorigenesis and have considerable theranostic potential. Rapid advances in bioinformatics indicate that miRNA may potentially interact with lncRNA to modulate their regulatory roles. miR-148b-3p has been reported to have a vital role in regulating tumor progression. However, the expression pattern of miR-148b-3p in glioma remains largely unknown, and interactions between miR-148b-3p and lncRNA has yet to be identified. The aim of the present study was to insight into the regulatory role of miR-148b-3p in glioma. Using online software, theHOTAIRgene was identified as a possible lncRNA target of miR-148b-3p in the present study. siRNA was used to suppress the expression ofHOTAIRand reverse transcription-quantitative polymerase chain reaction was used to detect the expression of miR-148b-3p. The results confirmed thatHOTAIRmRNA expression was inversely correlated with miR-148b-3p expression in A172 glioma cells. Furthermore, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to detect the viability of cells, flow cytometry was performed to test cell cycle and a matrigel invasion assay was performed to test cell invasion. The results showed thatHOTAIRpromotes factors associated with malignancy, including cell proliferation, cell cycle progression and invasion, whereas miR-148b-3p suppresses malignancy. Bioinformatics and luciferase reporter assays showed that miR-148b-3p modulatesHOTAIRexpression by directly targeting theHOTAIRgene sequence. In summary, the results indicated that miR-148b-3p inhibits malignant biological behaviors of glioma cells by directly targetingHOTAIR. The current data provide important evidence for understanding the key roles of the lncRNA miRNA functional network in glioma.
authors.affiliation: Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Guan
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Cell Biology, College of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Tian
authors.affiliation: Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tian
keywords: HOTAIR; Matrigel; glioblastoma; long non-coding RNA/microRNA interaction; miR-148b-3p