title: Adoptive Transfer of Myeloid-Derived Suppressor Cells and T Cells in a Prostate Cancer Model.
abstract: The adoptive transfer of immune cells for cancer, chronic infection, and autoimmunity is an emerging field that has shown promise in recent trials. The transgenic adenocarcinoma mouse prostate (TRAMP) is a classical mouse model of prostate cancer (PCa) and TRAMP cell lines were derived from a TRAMP mouse tumor. TRAMP-C2 is tumorigenic when subcutaneously (s.c.) grafted into syngeneic C57BL/6 host mice (Fosteret al., 1997). This protocol will describe the adoptive transfer of purified CD11b+Gr1+double positive (DP) myeloid-derived suppressor cells (MDSC) and CD3+T cells in the TRAMP-C2 prostate cancer mouse model in order to establish the intrinsic functionality of these immune cells and to determine their role in tumorigenesisin vivo(Yanet al., 2014).
authors.affiliation: The institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China.
authors.forename: Libo
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Obstetrics and Gynecology, Indiana University School of Medicine, Indianapolis, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu