title: Tristetraprolin functions in cytoskeletal organization during mouse oocyte maturation.
abstract: Tristetraprolin (TTP), a member of TIS11 family containing CCCH tandem zinc finger, is one of the best characterized RNA-binding proteins. However, to date, the role of TTP in mammalian oocytes remains completely unknown. In the present study, we report the altered maturational progression and cytokinesis, upon specific knockdown of TTP in mouse oocytes. Furthermore, by confocal scanning, we observe the failure to form cortical actin cap during meiosis of TTP-depleted oocytes. Loss of TTP in oocytes also results in disruption of meiotic spindle morphology and chromosome alignment. In support of these findings, incidence of aneuploidy is accordingly increased when TTP is abated in oocytes. Our results suggest that TTP as a novel cytoskeletal regulator is required for spindle morphology/chromosome alignment and actin polymerization in oocytes.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Center of Reproductive Medicine, Jinling Hospital, Nanjing, China.
authors.forename: Rujun
authors.initials: R
authors.lastname: Ma
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiong
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Shao-Chen
authors.initials: SC
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Gu
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D023902:Chromosome Pairing / Q000235:genetics; D048749:Cytokinesis; D005260:Female; D008540:Meiosis / Q000235:genetics; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D034622:RNA Interference; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism; D051816:Tristetraprolin / Q000235:genetics / Q000378:metabolism*
chemical_list: D051816:Tristetraprolin
keywords: TTP; cytoskeleton; meiosis; oocyte; reproduction