title: Serum PAI-1 and PAI-1 4G/5G Polymorphism in Hepatitis C Virus-Induced Cirrhosis and Hepatitis C Virus-Induced Hepatocellular Carcinoma Patients.
abstract: Association between variable agent-induced hepatocellular carcinoma (HCC) and both PAI-1 4G/5G polymorphism and plasminogen activator inhibitor (PAI-1) levels compared to healthy controls have been reported in earlier studies. We aimed to assess serum PAI-1 and PAI-1 4G/5G polymorphism in hepatitis C virus (HCV)-induced HCC, HCV-induced liver cirrhosis, and viral infection-free apparently healthy control subjects. Forty nine HCC, 52 cirrhosis, and 105 controls were genotyped for PAI-1 4G/5G using an allele-specific polymerase chain reaction analysis. In addition, for 31 HCC, 24 cirrhosis, and 28 controls, serum PAI-1 level was measured by enzyme-linked immunosorbent assay (ELISA). There was no significant difference in PAI-1 4G/5G genotype distribution between cirrhosis and controls (p = 0.33, p = 0.15, and p = 0.38 for the codominant, dominant, and recessive models, respectively) or between HCC and cirrhosis (p = 0.5, p = 0.24, and p = 0.69 for the codominant, dominant, and recessive models, respectively). Serum PAI-1 was significantly higher in cirrhosis than controls and significantly lower in HCC than cirrhosis (p < 0.001 for both). Serum PAI-1 did not differ significantly among the three PAI-1 4G/5G genotypes in controls, cirrhosis, and HCC (p = 0.29, p = 0.28, and p = 0.73 respectively). We documented higher serum PAI-1 in HCV-induced HCC than viral infection-free controls, but interestingly, lower than HCV-induced liver cirrhosis patients. This was not genotype related. Further studies will be needed to clearly elucidate the underlying mechanism.
authors.affiliation: 1 Department of Clinical Pathology, Faculty of Medicine, Menoufia University , Shebein ElKom, Egypt .
authors.forename: Rawhia H
authors.initials: RH
authors.lastname: El Edel
authors.affiliation: 1 Department of Clinical Pathology, Faculty of Medicine, Menoufia University , Shebein ElKom, Egypt .
authors.forename: Enas Said
authors.initials: ES
authors.lastname: Essa
authors.affiliation: 2 Department of Tropical Medicine, Faculty of Medicine, Menoufia University , Shebein ElKom, Egypt .
authors.forename: Abdallah S
authors.initials: AS
authors.lastname: Essa
authors.affiliation: 1 Department of Clinical Pathology, Faculty of Medicine, Menoufia University , Shebein ElKom, Egypt .
authors.forename: Sara A
authors.initials: SA
authors.lastname: Hegazy
authors.affiliation: 1 Department of Clinical Pathology, Faculty of Medicine, Menoufia University , Shebein ElKom, Egypt .
authors.forename: Dalia I
authors.initials: DI
authors.lastname: El Rowedy
mesh_terms: D000483:Alleles; D063646:Carcinogenesis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000235:genetics / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000302:isolation & purification*; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000235:genetics / Q000821:virology; D008113:Liver Neoplasms / Q000097:blood* / Q000235:genetics / Q000821:virology; D008297:Male; D008875:Middle Aged; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors
chemical_list: D017395:Plasminogen Activator Inhibitor 1; C051321:SERPINE1 protein, human
keywords: HCC; HCV; PAI-1; cirrhosis