title: Dietary resveratrol improves immunity but reduces reproduction of broodstock medaka Oryzias latipes (Temminck & Schlegel).
abstract: Here, we investigated the effect of dietary resveratrol (20, 40, and 80 µg/g BW/day) on cell-mediated immunity (activity of spleen phagocytes and proliferative response of lymphocytes) and reproductive parameters (egg and sperm quality, i.e. fecundity-total number of eggs produced by individual fish, fertility, embryo survival, and hatching rate) in medaka. Fish fed feed with resveratrol at 40 and 80 µg/g BW/day had significantly higher metabolic activity and intracellular phagocyte killing activity than control. The proliferative lymphocyte activity of the fish from R80 group was greater by more than 20 % in comparison with the control group (P < 0.05). The percentage of macrophages (MO) and their mean fluorescence intensities (MFI) in R40 and R80 groups were significantly higher compared to C and R20 groups (P < 0.05). The differences in MO and MFI values ranged from 52.5 % (±1.5; R0 group) to 65.8 % (±1.6; R80 group) and from 23.2 (±1.4; R0 group) to 38.2 (±2.4; R80 group), respectively. Moreover, resveratrol at 80 µg/g BW/day decreased liver COX activity, i.e. 5.4 in R80 group and 7.9 in R0 group (P < 0.05). The motility parameters of the sperm obtained from the males fed feed supplemented with resveratrol at 80 µg/g BW/day exhibited the highest values except the linearity, which was lower as compared to the control (P < 0.05). The results indicate that diet supplemented with resveratrol at a dosage of 40 µg/g BW/day improves phagocyte killing ability and lymphocyte proliferation in broodstock and accelerates offspring hatch. Also, the results suggest that COX activity influences sperm and oocyte quality in fish; the presence of a COX inhibitor in the dose of 40 µg/g BW/day decreased the embryo survival.
authors.affiliation: Department of Aquaculture, The Stanisław Sakowicz Inland Fisheries Institute, Oczapowskiego 10, 10-719, Olsztyn, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Kowalska
authors.affiliation: Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719, Olsztyn, Poland.
authors.forename: Andrzej K
authors.initials: AK
authors.lastname: Siwicki
authors.affiliation: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland. r.kowalski@pan.olsztyn.pl.
authors.forename: Radosław K
authors.initials: RK
authors.lastname: Kowalski
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004032:Diet / Q000662:veterinary*; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects; D007155:Immunologic Factors / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D009990:Oryzias / Q000276:immunology* / Q000502:physiology*; D010587:Phagocytosis / Q000187:drug effects; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects*; D000077185:Resveratrol; D013081:Sperm Motility / Q000187:drug effects; D013154:Spleen / Q000166:cytology; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D007155:Immunologic Factors; D013267:Stilbenes; D011451:Prostaglandin-Endoperoxide Synthases; D000077185:Resveratrol
keywords: Egg; Fish; Immunomodulators; Resveratrol; Sperm