title: Concentrated Hypoxia-Preconditioned Adipose Mesenchymal Stem Cell-Conditioned Medium Improves Wounds Healing in Full-Thickness Skin Defect Model.
abstract: In recent years, the bioactive factors were utilized in exercise and athletic skin injuries. In this research, the concentrated conditioned medium of hypoxia-preconditioned adipose mesenchymal stem cells, which is rich in bioactive factor, is applied in full-thickness skin defect model to evaluate the therapeutic efficacy. Adipose mesenchymal stem cells were harvested from the abdominal subcutaneous adipose tissues. The surface markers and the potential of differentiation were analyzed. The conditioned medium of hypoxia-preconditioned stem cells was collected and freeze-dried and then applied on the rat full-thickness skin defect model, and the healing time of each group was recorded. Haematoxylin and eosin staining of skin was assessed by microscope. The characteristics of adipose mesenchymal stem cells were similar to those of other mesenchymal stem cells. The concentration of protein in freeze-dried conditioned medium in 1 mL water was about 15 times higher than in the normal condition medium. In vivo, the concentrated hypoxia-preconditioned conditioned medium can reduce the wound size and accelerate the skin wound healing. The concentrated hypoxia-preconditioned adipose mesenchymal stem cell-conditioned medium has great effect on rat model of wound healing, and it would be an ideal agent for wound care in clinical application.
authors.affiliation: Department of Human Sports Science, Nanjing Institute of Physical Education, Nanjing 210014, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Sun
authors.affiliation: Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Guo
authors.affiliation: Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Human Sports Science, Nanjing Institute of Physical Education, Nanjing 210014, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Human Sports Science, Nanjing Institute of Physical Education, Nanjing 210014, China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Human Sports Science, Nanjing Institute of Physical Education, Nanjing 210014, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, China.
authors.forename: Yan
authors.identifier: 0000-0002-7940-6307
authors.initials: Y
authors.lastname: Xu