title: Modelling of microalgal growth and lipid production in Dunaliella tertiolecta using nitrogen-phosphorus-potassium fertilizer medium in sintered disk chromatographic glass bubble column.
abstract: A comprehensive mathematical model involving NPK-10:26:26 fertilizer, NaCl, NaHCO3, light and temperature operating variables for Dunaliella tertiolecta cultivation is formulated to predict microalgae-biomass and lipid productivity. Proposed model includes Monod/Andrews kinetics for the absorption of essential nutrients into algae-biomass and Droop model involving internal nutrient cell quota for microalgae growth, assuming algae-biomass is composed of sugar, functional-pool and neutral-lipid. Biokinetic model parameters are determined by minimizing the residual-sum-of-square-errors between experimental and computed microalgae-biomass and lipid productivity using genetic algorithm. Developed model is validated with the experiments of Dunaliella tertiolecta cultivation using air-agitated sintered-disk chromatographic glass-bubble column and the effects of operating variables on microalgae-biomass and lipid productivity is investigated. Finally, parametric sensitivity analysis is carried out to know the sensitivity of model parameters on the obtained results in the input parameter space. Proposed model may be helpful in scale-up studies and implementation of model-based control strategy in large-scale algal cultivation.
authors.affiliation: Department of Petroleum Engineering, Indian School of Mines, Dhanbad 826 004, India.
authors.forename: Anup
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Petroleum Engineering, Indian School of Mines, Dhanbad 826 004, India. Electronic address: cguria.che@gmail.com.
authors.forename: Chandan
authors.initials: C
authors.lastname: Guria
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology, Kanpur 208 016, India.
authors.forename: G
authors.initials: G
authors.lastname: Chitres
authors.affiliation: Department of Petroleum Engineering, Indian School of Mines, Dhanbad 826 004, India.
authors.forename: Arunangshu
authors.initials: A
authors.lastname: Chakraborty
authors.affiliation: Department of Petroleum Engineering, Indian School of Mines, Dhanbad 826 004, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Pathak
mesh_terms: D000465:Algorithms; D018533:Biomass; D002845:Chromatography / Q000379:methods*; D005308:Fertilizers; D058086:Microalgae / Q000254:growth & development*; D008962:Models, Theoretical
chemical_list: D005308:Fertilizers
keywords: Biomass-lipid productivity; Dunaliella tertiolecta; Kinetics; Modelling; NPK-10:26:26; Sintered disk chromatographic bubble column