title: Prevalence, Seasonal Occurrence, and Antimicrobial Resistance of Salmonella spp. Isolates Recovered from Chicken Carcasses Sampled at Major Poultry Processing Plants of South Korea.
abstract: The current study was conducted to assess Salmonella spp. contamination in chicken carcasses produced at major poultry processing plants in South Korea. In total, 120 chicken carcasses were collected through 12 individual trials (10 chickens per trial) from six poultry processing plants in the summer of 2014 and the winter of 2015. Eighteen chicken samples (15%) were contaminated with Salmonella, with a higher rate of contamination observed during summer (14 isolates, 11.7%) than during winter (four isolates, 3.3%). Salmonella enterica serotype Typhimurium was the most prevalent, followed by Salmonella Hadar, Salmonella Rissen, Salmonella Bareilly, and Salmonella Virchow. Among five multidrug resistant isolates, a single strain was resistant to 10 antibiotics, including third-generation cephalosporins. This cephalosporin-resistant strain exhibited the extended-spectrum β-lactamase (ESBL) phenotype and harbored the gene encoding CTX-M-15, the most prevalent ESBL enzyme worldwide. Herein, repetitive-sequence-based polymerase chain reaction (rep-PCR) subtyping was conducted to discriminate the isolated Salmonella spp. and the ESBL-producing Salmonella isolate was distinguished by rep-PCR molecular subtyping, showing low genetic similarity in their rep-PCR-banding patterns. Given that poultry processing plants are the last stage in the chicken-production chain, the occurrence of Salmonella spp. including ESBL-producing strain in individually packaged chicken products highlights the necessity for regular monitoring for Salmonella in poultry processing plants.
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, Korea.|2 Foreign Animal Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Korea.
authors.forename: Soo-Kyoung
authors.initials: SK
authors.lastname: Lee
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, Korea.
authors.forename: Dasom
authors.initials: D
authors.lastname: Choi
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, Korea.
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kim
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, Korea.
authors.forename: Dong-Hyeon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, Korea.
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002645:Chickens / Q000382:microbiology*; D052978:Disk Diffusion Antimicrobial Tests; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial; D005506:Food Contamination; D005513:Food Inspection; D018857:Food Packaging; D008460:Meat / Q000382:microbiology*; D008462:Meat-Packing Industry; D058889:Molecular Typing; D056910:Republic of Korea; D019779:Salmonella enterica / Q000145:classification / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D012621:Seasons; D062211:Spatio-Temporal Analysis; D018440:beta-Lactam Resistance
chemical_list: D000900:Anti-Bacterial Agents
keywords: ESBL; Salmonella; antibiotic resistance profile; chicken; subtyping