title: Comparative speed of kill of sarolaner (Simparica™ Chewables) and fluralaner (Bravecto(®)) against induced infestations of Amblyomma americanum on dogs.
abstract: The lone star tick, Amblyomma americanum, infests dogs and cats in North America and transmits the pathogens Ehrlichia chaffeensis and Ehrlichia ewingii, which cause monocytic and granulocytic ehrlichiosis in dogs and humans, and Cytauxzoon felis which causes cytauxzoonosis in cats. A parasiticide's speed of kill is important to minimize the direct deleterious effects [related to blood-feeding] of tick infestation and reduce the risk of transmission of tick-borne pathogens. In this study the speed of kill of sarolaner (Simparica™ Chewables) administered monthly for 3 months against A. americanum on dogs was evaluated and compared with a single dose of fluralaner (Bravecto(®)) for 13 weeks.
  
  
  
  Based on pretreatment tick counts, 24 dogs were randomly allocated to treatment with placebo or sarolaner at the label rate (2 to 4 mg/kg) on Days 0, 30 and 60 or with fluralaner (25 to 56 mg/kg) once according to manufacturer's instructions on Day 0. Dogs were examined and live ticks counted at 8, 12, and 24 h after treatment and subsequent re-infestations on Days 14, 28, 42, 58, 76 and 90. Acaricidal efficacy was determined at each time point relative to counts for placebo dogs.
  
  
  
  Monthly oral doses of sarolaner provided > 95 % efficacy within 24 h of treatment, and consistently provided > 70 % efficacy against subsequent re-infestations with ticks within 24 h over the entire treatment period. Significantly more live ticks were recovered from fluralaner-treated dogs than from sarolaner-treated dogs at 24 h after re-infestation from Day 42 onwards. At 24 h, efficacy of fluralaner was ≤ 20 % from Day 42 to the end of the study on Day 90. There were no adverse reactions to treatment.
  
  
  
  In this controlled laboratory evaluation, monthly treatment with sarolaner provided consistent efficacy against A. americanum with > 70 % of ticks killed within 24 h after a single oral dose over the duration of the study. Monthly treatment with sarolaner consistently killed significantly more ticks within 24 h than a single dose of fluralaner from 6 weeks after initial treatment.
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, 333 Portage St., Kalamazoo, MI, 49007, USA. robert.six@zoetis.com.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Six
authors.affiliation: YVRS, 7243 East Ave, Turlock, CA, 95380, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Young
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, 333 Portage St., Kalamazoo, MI, 49007, USA.
authors.forename: Melanie R
authors.initials: MR
authors.lastname: Myers
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, 333 Portage St., Kalamazoo, MI, 49007, USA.
authors.forename: Sean P
authors.initials: SP
authors.lastname: Mahabir
mesh_terms: D000818:Animals; D001384:Azetidines / Q000627:therapeutic use*; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D007306:Insecticides / Q000008:administration & dosage*; D007555:Isoxazoles / Q000627:therapeutic use*; D026863:Ixodidae / Q000187:drug effects*; D010919:Placebos / Q000008:administration & dosage; D013141:Spiro Compounds / Q000627:therapeutic use*; D013984:Tick Infestations / Q000188:drug therapy / Q000662:veterinary*; D016896:Treatment Outcome
chemical_list: C000591860:A1443 compound; D001384:Azetidines; D007306:Insecticides; D007555:Isoxazoles; D010919:Placebos; D013141:Spiro Compounds; C000623191:sarolaner
keywords: Amblyomma americanum; Bravecto®; Dog; Fluralaner; Isoxazoline; Oral; Sarolaner; Simparica™; Speed of kill; Tick