title: Combined PCR and Q-RT-PCR technique for detecting chimerism in a non-human Primate vascularized osteomyocutaneous allografts model.
abstract: Face transplantation and other composite tissue transplantation (CTA) are permissive to transplantation tolerance. The real reason, that composite tissue containing bone achieves transplantation immune tolerance more easily than the composite tissue without the bone is not clear. The chimerism may be the main mechanism in the progress of inducing the transplantation tolerance by CTA. We currently have established a non-human Primate Vascularized Osteomyocutaneous Allografts Model. To test the chimerism which comes from donor after the transplantation, we developed a method which combined reverse transcription polymerase chain reaction (RT-PCR) and real-time quantitative PCR (qRT-PCR) technique using primers specific for Macaca fascicularis sex determination region on the Y chromosome (SRY) gene. With the method, we estimated the level of the chimerism.
authors.affiliation: Taizhou people's Hospital Department of Neurosurgery China.
authors.forename: J
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Tongji Hospital Affiliated to Huazhong University of Science and Technology Organ Transplantation Research Institute China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: Zhongnan Hospital Transplantation Center of Wuhan University China.
authors.forename: F
authors.initials: F
authors.lastname: Zhong
authors.affiliation: Tongji Hospital Affiliated to Huazhong University of Science and Technology Organ Transplantation Research Institute China.
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.affiliation: East Hospital Research Center for Translation Medicine China liyuan_ly2015@sina.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Zhongnan Hospital Transplantation Center of Wuhan University China zhengxinxiao_zh@sina.com.
authors.forename: X X
authors.initials: XX
authors.lastname: Zheng
mesh_terms: D064591:Allografts; D000818:Animals; D016025:Bone Transplantation; D046528:Chimerism; D017931:DNA Primers / Q000378:metabolism; D018628:Gene Dosage; D016678:Genome; D006801:Humans; D008252:Macaca fascicularis; D008954:Models, Biological; D018919:Neovascularization, Physiologic; D009691:Nucleic Acid Denaturation; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D051876:Sex-Determining Region Y Protein / Q000235:genetics; D016038:Skin Transplantation
chemical_list: D017931:DNA Primers; D051876:Sex-Determining Region Y Protein