title: Reversal of the Migratory and Invasive Phenotype of Ras-Transfected Mammary Cells by Photodynamic Therapy Treatment.
abstract: Photodynamic therapy (PDT) is a non-thermal technique for inducing tumor damage following administration of a light-activated photosensitizing drug (PS). In a previous work we found that PDT induces cytoskeleton changes in HB4a-Ras cells (human mammary breast carcinoma HB4a cells transfected with the RAS oncogene). In the present work we have studied the migratory and invasive features and the expression of proteins related to these processes on HB4a-Ras cells after three successive cycles of PDT using different PSs: 5-aminolevulinic acid (ALA), Verteporfin (Verte), m-tetrahydroxyphenylchlorin (m-THPC), and Merocyanine 540 (MC). A slight (1.25- to 2-fold) degree of resistance was acquired in cell populations subjected to the three successive PDT treatments. However, complete cell killing was achieved after a light dose increase. Regardless of the PS employed, all the PDT-treated populations had shorter stress fibres than the untreated control HB4a-Ras cells, and the number of dorsal stress fibres was decreased in the PDT-treated populations. E-Cadherin distribution, which was already aberrant in HB4a-Ras cells, became even more diffuse in the PDT-treated populations, though its expression was increased in some of them. The strong migratory and invasive ability of HB4a-Ras cells in vitro was impaired in all the PDT-treated populations, with a behavior that was similar to the parental non-tumoral HB4a cells. MMP-2 and -9 metalloproteinase activities were also impaired in the PDT-treated populations. The evidence presented herein suggests that the cells surviving PDT would be less metastatic than the initial population. These findings encourage the use of PDT in combination with other treatments such as intraoperative or post-surgery therapeutic procedures. J. Cell. Biochem. 118: 464-477, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Hospital de Clínicas José de San Martín, CONICET, University of Buenos Aires, Av. Córdoba 2351 1er subsuelo, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Calvo
authors.affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Hospital de Clínicas José de San Martín, CONICET, University of Buenos Aires, Av. Córdoba 2351 1er subsuelo, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Daniel
authors.initials: D
authors.lastname: Sáenz
authors.affiliation: Instituto de Nanosistemas & CEDESI, Universidad Nacional de San Martín. 25 de Mayo y Francia, San Martín, Provincia de Buenos Aires, Argentina.
authors.forename: Marina
authors.initials: M
authors.lastname: Simian
authors.affiliation: Instituto de Oncología "Ángel H. Roffo", Av. San Martín 5481, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Rocío
authors.initials: R
authors.lastname: Sampayo
authors.affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Hospital de Clínicas José de San Martín, CONICET, University of Buenos Aires, Av. Córdoba 2351 1er subsuelo, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Leandro
authors.initials: L
authors.lastname: Mamone
authors.affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Hospital de Clínicas José de San Martín, CONICET, University of Buenos Aires, Av. Córdoba 2351 1er subsuelo, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Pablo
authors.initials: P
authors.lastname: Vallecorsa
authors.affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Hospital de Clínicas José de San Martín, CONICET, University of Buenos Aires, Av. Córdoba 2351 1er subsuelo, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Alcira
authors.initials: A
authors.lastname: Batlle
authors.affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Hospital de Clínicas José de San Martín, CONICET, University of Buenos Aires, Av. Córdoba 2351 1er subsuelo, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Adriana
authors.initials: A
authors.lastname: Casas
authors.affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Hospital de Clínicas José de San Martín, CONICET, University of Buenos Aires, Av. Córdoba 2351 1er subsuelo, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Di Venosa
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002461:Cell Line, Transformed; D005260:Female; D011905:Genes, ras; D006801:Humans; D042361:Mammary Glands, Human / Q000378:metabolism* / Q000473:pathology; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D014162:Transfection
chemical_list: D017319:Photosensitizing Agents
keywords: INVASION; METASTASIS; MIGRATION; PDT; PHOTODYNAMIC THERAPY; RAS ONCOGENE