title: Characterization of gelatin/chitosan scaffold blended with aloe vera and snail mucus for biomedical purpose.
abstract: Biologically active scaffolds used in tissue engineering and regenerative medicine have been generating promising results in skin replacement. The present study aims to test the hypothesis that the incorporation of Aloe vera and snail mucus into scaffolds based on gelatin and chitosan could improve their structure, composition and biodegradability, with a potential effect on bioactivity. Homogeneous pore diameter as well as pore walls in the composite scaffold could be seen in the SEM image. The pores in the scaffolds were interconnected and their sizes ranged from 93 to 296μm. The addition of Aloe vera and snail mucus enlarged the mean pore size with increased porosity and caused changes in the pore architecture. The FTIR analysis has shown good affinity and interaction between the matrix and the Aloe, which may decrease water-binding sites, so this fact hindered the water absorption capacity of the material. The mechanical properties could explain the highest swelling capacity of the snail scaffold, because the high percentage of elongation could facilitate the entry of liquid in it, generating a matrix with plenty of fluid retention. The real innovation in the present work could be the use of these substances (Aloe and snail mucus) for tissue engineering.
authors.affiliation: University of São Paulo. Depto de Eng. de Alimentos - FZEA - USP, Av. Duque de Caxias Norte, 225, CEP, 13635-900 Pirassununga, (SP), Brazil. Electronic address: daniel.lopez@usp.br.
authors.forename: Daniel Enrique
authors.initials: DE
authors.lastname: López Angulo
authors.affiliation: University of São Paulo. Depto de Eng. de Alimentos - FZEA - USP, Av. Duque de Caxias Norte, 225, CEP, 13635-900 Pirassununga, (SP), Brazil.
authors.forename: Paulo José
authors.initials: PJ
authors.lastname: do Amaral Sobral
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D005612:Freeze Drying; D005780:Gelatin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009093:Mucus / Q000737:chemistry*; D012908:Snails / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D034421:Sus scrofa; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000596:Amino Acids; D005780:Gelatin; D048271:Chitosan
keywords: Biocompatible scaffolds; Interconnected matrix; Tissue regeneration