title: Photoactivation of the Ni-SIr state to the Ni-SIa state in [NiFe] hydrogenase: FT-IR study on the light reactivity of the ready Ni-SIr state and as-isolated enzyme revisited.
abstract: The Ni-SIr state of [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F was photoactivated to its Ni-SIa state by Ar(+) laser irradiation at 514.5 nm, whereas the Ni-SL state was light induced from a newly identified state, which was less active than any other identified state and existed in the "as-isolated" enzyme.
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma-shi, Nara 630-0192, Japan. hirota@ms.naist.jp and CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Hulin
authors.initials: H
authors.lastname: Tai
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma-shi, Nara 630-0192, Japan. hirota@ms.naist.jp.
authors.forename: Liyang
authors.initials: L
authors.lastname: Xu
authors.affiliation: Graduate School of Life Science, University of Hyogo, 3-2-1 Koto Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
authors.forename: Seiya
authors.initials: S
authors.lastname: Inoue
authors.affiliation: Graduate School of Life Science, University of Hyogo, 3-2-1 Koto Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Nishikawa
authors.affiliation: CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan and Graduate School of Life Science, University of Hyogo, 3-2-1 Koto Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Higuchi
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma-shi, Nara 630-0192, Japan. hirota@ms.naist.jp and CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Hirota
mesh_terms: D016969:Desulfovibrio vulgaris / Q000201:enzymology*; D006864:Hydrogenase / Q000378:metabolism*; D008027:Light; D010777:Photochemistry
chemical_list: C043296:nickel-iron hydrogenase; D006864:Hydrogenase