title: Transcription factor c-jun regulates β3Gn-T8 expression in gastric cancer cell line SGC-7901.
abstract: Aberrant glycosylation, a common feature of malignant alteration, is partly due to changes in the expression of glycosyltransferases, including β1,3-N-acetyl-glucosaminyltrans-ferase 8 (β3Gn‑T8), which synthesizes poly-N-acetyllactosamine (poly-LacNAc) chains on β1,6 branched N‑glycans. Although the role of β3Gn‑T8 in tumors has been reported, the regulation of β3Gn‑T8 expression, however, is still poorly understood. In the present study, we used three online bioinformatic software tools to identify multiple c‑jun binding sites in the promoter of the β3Gn‑T8 gene. Using luciferase reporter assay, chromatin immunoprecipitation (ChIP) analysis, RT‑PCR and western blot analysis, we revealed that c‑jun could bind to and activate the β3Gn‑T8 promoter, thus upregulating β3Gn‑T8 expression. This was also confirmed by changes in β3Gn‑T8 activity as demonstrated by flow cytometry, immunofluorescence and lectin blot analysis using LEA lectin. Moreover, expression of glycoprotein HG‑CD147, the substrate of β3Gn‑T8, was also regulated by c‑jun. In addition, c‑jun and β3Gn‑T8 were more highly expressed in the gastric cancer tissues when compared to these levels in the adjacent non‑tumor gastric tissues, and β3Gn‑T8 expression was positively correlated with c‑jun expression. These results suggest that c‑jun plays a significant role in regulating the expression of β3Gn‑T8 in the SGC‑7901 cell line and may be involved in the development of malignancy via the activity of β3Gn‑T8.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Inspection, Suzhou Health College, Suzhou, Jiangsu 215001, P.R. China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Suzhou Cancer Center Core Laboratory, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215001, P.R. China.
authors.forename: Shitao
authors.initials: S
authors.lastname: Zou
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Ni
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Hua
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Shiliang
authors.initials: S
authors.lastname: Wu
mesh_terms: D001665:Binding Sites / Q000235:genetics; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics; D006031:Glycosylation; D006801:Humans; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D006023:Glycoproteins; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; C496333:B3GNT8 protein, human; D017351:N-Acetylglucosaminyltransferases