title: Holographic Assessment of Lymphoma Tissue (HALT) for Global Oncology Field Applications.
abstract: Low-cost, rapid and accurate detection technologies are key requisites to cope with the growing global cancer challenges. The need is particularly pronounced in resource-limited settings where treatment opportunities are often missed due to the absence of timely diagnoses. We herein describe a Holographic Assessment of Lymphoma Tissue (HALT) system that adopts a smartphone as the basis for molecular cancer diagnostics. The system detects malignant lymphoma cells labeled with marker-specific microbeads that produce unique holographic signatures. Importantly, we optimized HALT to detect lymphomas in fine-needle aspirates from superficial lymph nodes, procedures that align with the minimally invasive biopsy needs of resource-constrained regions. We equipped the platform to directly address the practical needs of employing novel technologies for "real world" use. The HALT assay generated readouts in <1.5 h and demonstrated good agreement with standard cytology and surgical pathology.
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;; 2. Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
authors.forename: Divya
authors.initials: D
authors.lastname: Pathania
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;; 2. Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
authors.forename: Hyungsoon
authors.initials: H
authors.lastname: Im
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;; 2. Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
authors.forename: Aoife
authors.initials: A
authors.lastname: Kilcoyne
authors.affiliation: 3. Department of Pathology, Massachusetts General Hospital, Boston, MA, 02114;
authors.forename: Aliyah R
authors.initials: AR
authors.lastname: Sohani
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
authors.forename: Lioubov
authors.initials: L
authors.lastname: Fexon
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;; 2. Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
authors.forename: Misha
authors.initials: M
authors.lastname: Pivovarov
authors.affiliation: 4. Center for Lymphoma, Massachusetts General Hospital, Boston, MA, 02114;; 5. Massachusetts General Hospital Cancer Center, Boston, MA 02114;; 6. Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
authors.forename: Jeremy S
authors.initials: JS
authors.lastname: Abramson
authors.affiliation: 5. Massachusetts General Hospital Cancer Center, Boston, MA 02114;; 7. Global Oncology Initiative, Dana-Farber / Harvard Cancer Center, Boston, MA, 02115;; 8. Department of Obstetrics, Gynecology and Reproductive Biology, Massachusetts General Hospital, Boston, MA 02114;
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Randall
authors.affiliation: 5. Massachusetts General Hospital Cancer Center, Boston, MA 02114;; 6. Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Chabner
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;; 2. Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;; 9. Department of Systems Biology, Harvard Medical School, Boston, MA 02115.
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;; 2. Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
authors.forename: Hakho
authors.initials: H
authors.lastname: Lee
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;; 5. Massachusetts General Hospital Cancer Center, Boston, MA 02114;; 6. Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
authors.forename: Cesar M
authors.initials: CM
authors.lastname: Castro
mesh_terms: D044963:Biopsy, Fine-Needle; D006696:Holography / Q000379:methods*; D008198:Lymph Nodes / Q000473:pathology; D008223:Lymphoma / Q000175:diagnosis* / Q000473:pathology; D008863:Microspheres; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D000068997:Smartphone; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
keywords: Cancer diagnostics; Point-of-care diagnostics; Smartphone testing; global oncology.