title: First Occurrence of OXA-72-Producing Acinetobacter baumannii in Serbia.
abstract: Here, we characterized the first OXA-72-producing Acinetobacter baumannii isolate (designated MAL) recovered from a urine sample from a Serbian patient. Antimicrobial susceptibility testing, plasmid analysis, and whole-genome sequencing (WGS) were performed to fully characterize the resistome of the A. baumannii MAL clinical isolate. The isolate was multidrug resistant and remained susceptible only to colistin and tigecycline. PCR analysis revealed the presence of the carbapenemase OXA-72, an OXA-40 variant. Extraction by the Kieser method revealed the presence of two plasmids, and one of these, a ca. 10-kb plasmid, harbored the blaOXA-72 gene. WGS revealed 206 contigs corresponding to a genome of 3.9 Mbp in size with a G+C content of 38.8%. The isolate belonged to sequence type 492 and to worldwide clone II (WWCII). Naturally occurring β-lactamase-encoding genes (blaADC-25 and blaOXA-66) were also identified. Aminoglycoside resistance genes encoding one aminoglycoside adenyltransferase (aadA2), three aminoglycoside phosphatases (strA, strB, aphA6), and one 16S RNA methylase (armA) conferring resistance to all aminoglycosides were identified. Resistance to fluoroquinolones was likely due to mutations in gyrA, parC, and parE Of note, the resistome matched perfectly with the antibiotic susceptibility testing results.
authors.affiliation: Bacteriology-Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France French National Reference Center for Antibiotic Resistance: Carbapenemase Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France EA7391 (Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases), Faculty of Medicine, University Paris-Sud, University Paris-Saclay, Le Kremlin-Bicêtre, France laurent.dortet@aphp.fr.
authors.forename: Laurent
authors.initials: L
authors.lastname: Dortet
authors.affiliation: French National Reference Center for Antibiotic Resistance: Carbapenemase Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France EA7391 (Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases), Faculty of Medicine, University Paris-Sud, University Paris-Saclay, Le Kremlin-Bicêtre, France.
authors.forename: Rémy A
authors.identifier: 0000-0002-2307-3232
authors.initials: RA
authors.lastname: Bonnin
authors.affiliation: Bacteriology-Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France French National Reference Center for Antibiotic Resistance: Carbapenemase Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France EA7391 (Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases), Faculty of Medicine, University Paris-Sud, University Paris-Saclay, Le Kremlin-Bicêtre, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Bernabeu
authors.affiliation: Infectious Diseases Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France.
authors.forename: Lélia
authors.initials: L
authors.lastname: Escaut
authors.affiliation: Infectious Diseases Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Vittecoq
authors.affiliation: EA7391 (Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases), Faculty of Medicine, University Paris-Sud, University Paris-Saclay, Le Kremlin-Bicêtre, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Girlich
authors.affiliation: Molecular Genetics and Hormonology Department, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France.
authors.forename: Dilek
authors.initials: D
authors.lastname: Imanci
authors.affiliation: Bacteriology-Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France French National Reference Center for Antibiotic Resistance: Carbapenemase Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France EA7391 (Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases), Faculty of Medicine, University Paris-Sud, University Paris-Saclay, Le Kremlin-Bicêtre, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Fortineau
authors.affiliation: Bacteriology-Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France French National Reference Center for Antibiotic Resistance: Carbapenemase Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France EA7391 (Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases), Faculty of Medicine, University Paris-Sud, University Paris-Saclay, Le Kremlin-Bicêtre, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Naas
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D055771:Serbia; D014552:Urinary Tract Infections / Q000188:drug therapy / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C000596363:OXA-72 carbapenemase, Acinetobacter baumannii; D001618:beta-Lactamases