title: Collagen-graft mixed cellulose esters membrane maintains undifferentiated morphology and markers of potential pluripotency in feeder-free culture of induced pluripotent stem cells.
abstract: Induced pluripotent stem cells (iPSCs) are unique and unlimited clinical sources of stem cell therapy for the regenerative medicine. Feeder layer preparation is an important step for iPSCs production, which is expensive, time-consuming and requires conversance. In the present study, we investigated the maintenance of pluripotency, and stemness of the iPSCs through feeder-free culture on a collagen-grafted Mixed Cellulose Esters membrane (MCE-COL) after three passages during twelve days. Results have demonstrated that the iPSCs cultured on MCE-COL membrane had a fine, typical undifferentiated morphology, increased proliferation rate and significant multi-lineage differentiation potential. Alkaline phosphatase (ALP) staining and pluripotency associated gene markers expression further confirmed that iPSCs cultured on the surface of MCE-COL had more ALP positive colonies and enhanced expression of Oct-4, Nanog, Sox-2 and ALP in comparison with MCE and control groups. Since MCE-COL membrane has three dimensional structure and bioactivity, it has the potential for usage in the feeder-free culture of iPSCs, and could be a suitable candidate to use as a feeder layer in stem cells preparation.
authors.affiliation: Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Cell Culture Media, Danesh Azma Cell Company, Tehran, Iran.
authors.forename: Sadegh
authors.initials: S
authors.lastname: Lotfalah Moradi
authors.affiliation: Department of Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Hajishafieeha
authors.affiliation: Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Shahrzad
authors.initials: S
authors.lastname: Nojedehi
authors.affiliation: Department of Physiotherapy, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
authors.forename: Vida
authors.initials: V
authors.lastname: Dinarvand
authors.affiliation: Islamic Azad University, Garmsar Branch, Garmsar, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Hesami Tackallou
authors.affiliation: Center for Research on Environmental Disease and Graduate Center for Toxicology, University of Kentucky, Lexington, KY, USA.
authors.forename: Ram V
authors.initials: RV
authors.lastname: Roy
authors.affiliation: Stem Cell Technology Research Center, Tehran, Iran; Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Ardeshirylajimi
authors.affiliation: Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: soleim_m@modares.ac.ir.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D000943:Antigens, Differentiation / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D008567:Membranes, Artificial
chemical_list: D000943:Antigens, Differentiation; D008567:Membranes, Artificial; C020253:cellulose ester plastic; D002482:Cellulose; D003094:Collagen
keywords: Feeder layer; Induced pluripotent stem cells; Mixed cellulose esters membrane; Three dimensional culture