title: Ratio of microRNA-122/155 in isoniazid-induced acute liver injury in mice.
abstract: Liver injury is a major hindrance to the treatment of tuberculosis (TB) patients due to the primary side effects associated with anti-TB drugs. Several investigations have identified sensitive biomarkers for the early diagnosis of anti-TB drug-induced liver injury (ADLI), including the use of microRNAs (miRNAs/miRs). However, the association between miR-122/155 and ADLI remains unknown. Thus, the present study used reverse transcription-quantitative polymerase chain reaction to observe changes in tissue miR-122/155 expression levels during the course of liver injury in mice. Liver injury was induced by the administration of isoniazid (INH), a first-line anti-TB drug. miR-122/155 expression levels were quantified at seven time points throughout 1 day (0.25, 0.75, 1.5, 6, 12, 18 and 24 h) based on the pharmacokinetics of INH in mice. Notably, over the timecourse of INH-induced liver injury, the tissue miR-122 expression level significantly decreased at 0.25 h, which is the peak concentration time of INH, compared with the control group (P<0.05). The change was more rapid than that of the serum aminotransferase and miR-155, which were significantly increased at 0.75 h. In addition, the pathological score correlated with the ratio of miR-122/miR-155 (r=-0.779; P<0.01). In conclusion, the miR-122/155 ratio may be utilized as a sensitive biomarker for ADLI, which could contribute to the early diagnosis of patients following anti-TB treatment.
authors.affiliation: Key Laboratory of Coal Mine Health and Safety, School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China; Bayan Nur Center for Disease Control and Prevention, Bayan Nur, Inner Mongolia 015000, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Song
authors.affiliation: Key Laboratory of Coal Mine Health and Safety, School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China.
authors.forename: Zhongrui
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Coal Mine Health and Safety, School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Coal Mine Health and Safety, School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China.
authors.forename: Xuebin
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Coal Mine Health and Safety, School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: He
authors.affiliation: Key Laboratory of Coal Mine Health and Safety, School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Key Laboratory of Coal Mine Health and Safety, School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Key Laboratory of Coal Mine Health and Safety, School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China.
authors.forename: Fumin
authors.initials: F
authors.lastname: Feng
keywords: antituberculosis drug-induced liver injury; epigenetics; isoniazid; microRNA-122; microRNA-155; microRNAs