title: On the adsorption of magnetite nanoparticles on lysozyme amyloid fibrils.
abstract: An adsorption of magnetic nanoparticles (MNP) from electrostatically stabilized aqueous ferrofluids on amyloid fibrils of hen egg white lysozyme (HEWL) in 2mg/mL acidic dispersions have been detected for the MNP concentration range of 0.01-0.1vol.%. The association of the MNP with amyloid fibrils has been characterized by transmission electron microscopy (TEM), small-angle X-ray scattering (SAXS) and magneto-optical measurements. It has been observed that the extent of adsorption is determined by the MNP concentration. When increasing the MNP concentration the formed aggregates of magnetic particles repeat the general rod-like structure of the fibrils. The effect is not observed when MNP are mixed with the solution of lysozyme monomers. The adsorption has been investigated with the aim to clarify previously found disaggregation activity of MNP in amyloid fibrils dispersions and to get deeper insight into interaction processes between amyloids and MNP. The observed effect is also discussed with respect to potential applications for ordering lysozyme amyloid fibrils in a liquid crystal phase under external magnetic fields.
authors.affiliation: Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Kosice, Slovakia.
authors.forename: Jozefina
authors.initials: J
authors.lastname: Majorosova
authors.affiliation: Joint Institute for Nuclear Research, Joliot-Curie 6, 141980 Dubna, Moscow Region, Russia; Kyiv Taras Shevchenko National University, Volodymyrska Street 64, 01033 Kyiv, Ukraine. Electronic address: vip@nf.jinr.ru.
authors.forename: Viktor I
authors.initials: VI
authors.lastname: Petrenko
authors.affiliation: Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Kosice, Slovakia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Siposova
authors.affiliation: Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Kosice, Slovakia.
authors.forename: Milan
authors.initials: M
authors.lastname: Timko
authors.affiliation: Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Kosice, Slovakia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Tomasovicova
authors.affiliation: Helmholtz-Zentrum Geesthacht: Centre for Materials and Coastal Research, Max-Planck-Street 1, 21502 Geesthacht, Germany.
authors.forename: Vasil M
authors.initials: VM
authors.lastname: Garamus
authors.affiliation: Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznań, Poland.
authors.forename: Marceli
authors.initials: M
authors.lastname: Koralewski
authors.affiliation: Joint Institute for Nuclear Research, Joliot-Curie 6, 141980 Dubna, Moscow Region, Russia.
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Avdeev
authors.affiliation: Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznań, Poland.
authors.forename: Błażej
authors.initials: B
authors.lastname: Leszczynski
authors.affiliation: NanoBiomedical Centre Adam Mickiewicz University, Umultowska 85, 61-614 Poznań, Poland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Jurga
authors.affiliation: Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Kosice, Slovakia.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Gazova
authors.affiliation: Institute of Physics, Academia Sinica, 128 Sec.2, Academia Rd., Nankang, Taipei 11529, Taiwan.
authors.forename: Shura
authors.initials: S
authors.lastname: Hayryan
authors.affiliation: Institute of Physics, Academia Sinica, 128 Sec.2, Academia Rd., Nankang, Taipei 11529, Taiwan.
authors.forename: Chin-Kun
authors.initials: CK
authors.lastname: Hu
authors.affiliation: Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Kosice, Slovakia.
authors.forename: Peter
authors.initials: P
authors.lastname: Kopcansky
mesh_terms: D000327:Adsorption; D000682:Amyloid / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002645:Chickens; D005260:Female; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D053838:Scattering, Small Angle; D055672:Static Electricity; D014961:X-Ray Diffraction
chemical_list: D000682:Amyloid; D058185:Magnetite Nanoparticles; C421864:hen egg lysozyme; D009113:Muramidase
keywords: Amyloid fibrils; Faraday rotation; Interaction of nanoparticles with biological molecules; Lysozyme; Magnetic nanoparticles; Small-angle X-ray scattering; Transmission electron microscopy