title: 4.1N is involved in a flotillin-1/β-catenin/Wnt pathway and suppresses cell proliferation and migration in non-small cell lung cancer cell lines.
abstract: The membrane-cytoskeletal protein 4.1N has recently been proposed as a tumor suppressor in a number of cancers of epithelial origin, including non-small-cell lung cancer (NSCLC). However, the molecular mechanism associated with 4.1N tumor suppression remains has not been thoroughly characterized. In this study, 4.1N was shown to directly interact with the lipid raft marker flotillin-1 through its FERM and U2 domains in several different NSCLC cell lines using immunoprecipitation, co-immunoprecipitation and pull-down assays. Moreover, 4.1N silencing/overexpression experiments in paired 95C/95D cells that are of homologous origin but varying endogenous 4.1N expression (high expression in 95C cells, low expression in 95D cells) indicated that 4.1N is involved in the suppression of cell proliferation and migration through a flotillin-1/β-catenin/Wnt pathway. Taken together, the findings of this study help to elucidate the novel tumor suppressor role of 4.1N in NSCLC.
authors.affiliation: State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, 110 Xiangya Road, Changsha, 410078, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, 110 Xiangya Road, Changsha, 410078, China. zhumin0678@yahoo.com.
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, 110 Xiangya Road, Changsha, 410078, China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, 110 Xiangya Road, Changsha, 410078, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, 110 Xiangya Road, Changsha, 410078, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, 110 Xiangya Road, Changsha, 410078, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of pathology, Central South University, 110 Xiangya Road, Changsha, 410078, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, 110 Xiangya Road, Changsha, 410078, China.
authors.forename: Weixin
authors.initials: W
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, 110 Xiangya Road, Changsha, 410078, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D060449:Wnt Signaling Pathway / Q000235:genetics*; D051176:beta Catenin / Q000235:genetics* / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D009479:Neuropeptides; D051176:beta Catenin; C120557:erythrocyte membrane protein band 4.1-like 1; C106354:flotillins
keywords: 4.1N, non-small cell lung cancer (NSCLC); Flotillin-1; Flotillin-1/β-catenin/Wnt pathway