title: [Correlation between MALDI-TOF Vitek-MSTM system and conventional identification methods of gastrointestinal infection causing bacteria].
abstract: OBJECTIVE
  Rapid identification of pathogens is essential for the diagnosis of gastrointestinal infections. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry has shown to be effective and fast for the identification of microorganisms. The objective of this study was to evaluate the correlation between Vitek-MSTM and conventional methods for bacterial identification causing gastrointestinal infection.
  
  
  METHODS
  A total of 329 gastrointestinal pathogens were identified using Vitek-MSTM (v2 SARAMIS MS -ID, bioMérieux, Marcy-I´Étoile, France) and routine diagnostic methods simultaneously. In cases of discrepancy 16SrRNA gene sequencing was performed.
  
  
  RESULTS
  The correlation between Vitek-MSTM and diagnostic methods was 100% except for Yersinia enterocolitica (94.1%), Helicobacter pylori (10%) and Aeromonas veronii (0 %).
  
  
  CONCLUSIONS
  Vitek-MSTM is a quick and useful method for identification of enterophatogenic bacteria. It is necessary to improve the performance of the system for the identification of H. pylori and A. veronii.
authors.affiliation: Carlos Ruiz de Alegría-Puig, Hospital Universitario Marqués de Valdecilla-IDIVAL (Servicio de Microbiología), Avda.Valdecilla s/n Cp: 39008. Santander, Spain. carlosrdap@hotmail.com.
authors.forename: C
authors.initials: C
authors.lastname: Ruiz de Alegría-Puig
authors.forename: A
authors.initials: A
authors.lastname: Aguirre-Quiñonero
authors.forename: J
authors.initials: J
authors.lastname: Agüero-Balbín
authors.forename: M P
authors.initials: MP
authors.lastname: Roiz-Mesones
authors.forename: L
authors.initials: L
authors.lastname: Martínez-Martínez
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000235:genetics; D005767:Gastrointestinal Diseases / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D007239:Infections / Q000382:microbiology*; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S