title: Cell wall composition and biofilm formation of azoles-susceptible and -resistant Candida glabrata strains.
abstract: In the present study, three strains of Candida glabrata have been investigated to shed light on the mechanisms involved in azole resistance during adherence and biofilm formation. In particular, a clinical isolate, susceptible to azole-based drugs, DSY562 and two different resistant mutagenic strains deriving from DSY562, SFY114 and SFY115, have been analysed with different approaches for their cell wall composition and properties. A proteomic analysis revealed that the expression of six cell wall-related proteins and biofilm formation varied between the strains. The SFY114 and SFY115 strains resulted to be less hydrophobic than the susceptible parental counterpart DSY562, on the other hand they showed a higher amount in total cell wall polysaccharides fraction in the total cell wall. Accordingly to the results obtained from the hydrophobicity and adherence assays, in the resistant strain SFY115 the biofilm formation decreased compared to the parental strain DSY562. Finally, the total glucose amount in resistant SFY115 was about halved in comparison to other strains. Taken together all these data suggest that azole drugs may affect the cell wall composition of C. glabrata, in relation to the different pathogenic behaviours.
authors.affiliation: a CNR-ICRM, c/o Institute of Biochemistry and Clinical Biochemistry , Catholic University , Largo F. Vito 1, Rome 00168 , Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Vitali
authors.affiliation: b Department of Public Health and Infectious Diseases , "Sapienza" University of Rome , P.le Aldo Moro 5, Rome 00161 , Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Vavala
authors.affiliation: c Institute of Biochemistry and Clinical Biochemistry , Catholic University , Largo F. Vito 1, Rome 00168 , Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Marzano
authors.affiliation: b Department of Public Health and Infectious Diseases , "Sapienza" University of Rome , P.le Aldo Moro 5, Rome 00161 , Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Leone
authors.affiliation: c Institute of Biochemistry and Clinical Biochemistry , Catholic University , Largo F. Vito 1, Rome 00168 , Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnola
authors.affiliation: c Institute of Biochemistry and Clinical Biochemistry , Catholic University , Largo F. Vito 1, Rome 00168 , Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Iavarone
authors.affiliation: b Department of Public Health and Infectious Diseases , "Sapienza" University of Rome , P.le Aldo Moro 5, Rome 00161 , Italy.
authors.forename: Letizia
authors.initials: L
authors.lastname: Angiolella
mesh_terms: D000268:Adhesiveness; D000935:Antifungal Agents / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D041221:Candida glabrata / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D002177:Candidiasis / Q000188:drug therapy / Q000382:microbiology; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism*; D026141:Drug Resistance, Multiple, Fungal; D062610:Fungal Polysaccharides / Q000096:biosynthesis; D005656:Fungal Proteins / Q000096:biosynthesis*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D057927:Hydrophobic and Hydrophilic Interactions; D008826:Microbial Sensitivity Tests; D009154:Mutation; D040901:Proteomics / Q000379:methods; D013045:Species Specificity; D013499:Surface Properties; D014230:Triazoles / Q000494:pharmacology*; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D000935:Antifungal Agents; D001393:Azoles; D062610:Fungal Polysaccharides; D005656:Fungal Proteins; D014230:Triazoles
keywords: Adherence; Biofilm; Candida glabrata; Cell wall; Drug-resistance; Hydrophobicity; Polysaccharides; Proteomic