title: Essential Oil of Ocimum basilicum L. and (-)-Linalool Blocks the Excitability of Rat Sciatic Nerve.
abstract: The racemate linalool and its levogyrus enantiomer [(-)-LIN] are present in many essential oils and possess several pharmacological activities, such as antinociceptive and anti-inflammatory. In this work, the effects of essential oil obtained from the cultivation of the Ocimum basilicum L. (EOOb) derived from Germplasm Bank rich in (-)-LIN content in the excitability of peripheral nervous system were studied. We used rat sciatic nerve to investigate the EOOb and (-)-LIN effects on neuron excitability and the extracellular recording technique was used to register the compound action potential (CAP). EOOb and (-)-LIN blocked the CAP in a concentration-dependent way and these effects were reversible after washout. EOOb blocked positive amplitude of 1st and 2nd CAP components with IC50 of 0.38 ± 0.2 and 0.17 ± 0.0 mg/mL, respectively. For (-)-LIN, these values were 0.23 ± 0.0 and 0.13 ± 0.0 mg/mL. Both components reduced the conduction velocity of CAP and the 2nd component seems to be more affected than the 1st component. In conclusion EOOb and (-)-LIN inhibited the excitability of peripheral nervous system in a similar way and potency, revealing that the effects of EOOb on excitability are due to the presence of (-)-LIN in the essential oil.
authors.affiliation: Physiology Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, Brazil.
authors.forename: Antonio
authors.initials: A
authors.lastname: Medeiros Venancio
authors.affiliation: Superior Institute of Biomedical Science, State University of Ceará, Campus of Itaperi, 60.740-000 Fortaleza, CE, Brazil.
authors.forename: Francisco Walber
authors.identifier: 0000-0003-2012-2040
authors.initials: FW
authors.lastname: Ferreira-da-Silva
authors.affiliation: Superior Institute of Biomedical Science, State University of Ceará, Campus of Itaperi, 60.740-000 Fortaleza, CE, Brazil.
authors.forename: Kerly Shamyra
authors.initials: KS
authors.lastname: da Silva-Alves
authors.affiliation: Physiology Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, Brazil.
authors.forename: Hugo
authors.initials: H
authors.lastname: de Carvalho Pimentel
authors.affiliation: Physiology Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, Brazil.
authors.forename: Matheus
authors.initials: M
authors.lastname: Macêdo Lima
authors.affiliation: Physiology Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, Brazil.
authors.forename: Michele
authors.initials: M
authors.lastname: Fraga de Santana
authors.affiliation: Chemistry Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, Brazil.
authors.forename: Péricles
authors.identifier: 0000-0002-8955-9614
authors.initials: P
authors.lastname: Barreto Alves
authors.affiliation: Chemistry Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, Brazil.
authors.forename: Givanildo
authors.initials: G
authors.lastname: Batista da Silva
authors.affiliation: Superior Institute of Biomedical Science, State University of Ceará, Campus of Itaperi, 60.740-000 Fortaleza, CE, Brazil.
authors.forename: José Henrique
authors.identifier: 0000-0002-3972-1361
authors.initials: JH
authors.lastname: Leal-Cardoso
authors.affiliation: Physiology Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, Brazil.
authors.forename: Murilo
authors.initials: M
authors.lastname: Marchioro