title: Genome-Wide Association Mapping of Rice Resistance Genes Against Magnaporthe oryzae Isolates from Four African Countries.
abstract: Rice blast disease is emerging as a major constraint to rice production in Africa. Although a traditional gene-tagging strategy using biparental crosses can effectively identify resistance (R) genes or quantitative trait loci (QTL) against Magnaporthe oryzae, the mapping procedure required is time consuming and requires many populations to investigate the genetics of resistance. In this report, we conducted a genome-wide association study (GWAS) to rapidly map rice genes conferring resistance against eight M. oryzae isolates from four African countries. We inoculated 162 rice cultivars, which were part of the rice diversity panel 1 (RDP1) and were previously genotyped with the 44,000 single-nucleotide polymorphism (SNP) chip, with the eight isolates. The GWAS identified 31 genomic regions associated with blast resistance (RABR) in the rice genome. In addition, we used polymerase chain reaction analysis to confirm the association between the Pish gene and a major RABR on chromosome 1 that was associated with resistance to four M. oryzae isolates. Our study has demonstrated the power of GWAS for the rapid identification of rice blast R or QTL genes that are effective against African populations of M. oryzae. The identified SNP markers associated with RABR can be used in breeding for resistance against rice blast in Africa.
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Emmanuel M
authors.initials: EM
authors.lastname: Mgonja
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Elias G
authors.initials: EG
authors.lastname: Balimponya
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Houxiang
authors.initials: H
authors.lastname: Kang
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Maria
authors.initials: M
authors.lastname: Bellizzi
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Chan Ho
authors.initials: CH
authors.lastname: Park
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Robert
authors.initials: R
authors.lastname: Mabagala
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Clay
authors.initials: C
authors.lastname: Sneller
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Jim
authors.initials: J
authors.lastname: Correll
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Stephen
authors.initials: S
authors.lastname: Opiyo
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Talbot
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Thomas
authors.initials: T
authors.lastname: Mitchell
authors.affiliation: First, fourth, fifth, sixth, tenth, twelfth, and thirteenth authors: Department of Plant Pathology, and second and eighth authors: Department of Horticulture and Crop Science, The Ohio State University, Columbus; third and thirteenth authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing; seventh author: Department of Crop Science and Production, Sokoine University of Agriculture, Morogoro, Tanzania; ninth author: Department of Plant Pathology, University of Arkansas, Fayetteville; and eleventh author: School of Biosciences, University of Exeter, UK.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Wang
mesh_terms: D000349:Africa; D001947:Breeding; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D055106:Genome-Wide Association Study; D005838:Genotype; D020082:Magnaporthe / Q000502:physiology*; D012275:Oryza / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D040641:Quantitative Trait Loci / Q000235:genetics*