title: Serum and bone pentosidine in patients with low impact hip fractures and in patients with advanced osteoarthritis.
abstract: Femoral neck fractures are a common occurrence in patients suffering from osteoporosis, while intracapsular hip fracture is rare in cases of osteoarthritis of the hip. Previous histomorphometric studies have emphasized the association between bone microarchitecture and the risk of low-impact fractures in osteoarthritis and osteoporosis patients. However, the strength of bone material is also a function of composition of organic bone matrix. In order to compare tissue material properties in these two clinical conditions, serum and bone pentosidine, a non-enzymatic collagen crosslinking element, was measured in patients who suffered a low-impact fracture, and in patients with advanced osteoarthritis.
  
  
  
  The patient population consisted of 70 patients who underwent hemiarthroplasty surgery for a femoral neck fracture, and 41 patients with advanced hip joint osteoarthritis without a history of low- impact fracture, who were indicated for total hip joint replacement. Pentosidine content was analyzed in bone samples and in serum obtained from fracture and osteoarthritis patients using high performance liquid chromatography.
  
  
  
  Serum and bone concentrations of pentosidine were higher in subjects with hip fractures compared with osteoarthritis after adjustment for age, sex, weight, serum creatinine, and diabetes. A significant positive correlation was found between bone and serum pentosidine in fractured cases. A comparable relationship was also demonstrated for pentosidine levels in serum and bone relative to differentiation of fracture and osteoarthritis cases.
  
  
  
  Serum pentosidine can be considered a potential biomarker for identification of subjects with impaired bone quality and bone strength.
authors.affiliation: Department of Orthopedics, Faculty of Medicine 1, Charles University Prague, and Bulovka Hospital, Prague, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Vaculík
authors.affiliation: Department of Composites and Carbon Materials, Institute of Rock Structure and Mechanics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Braun
authors.affiliation: Department of Orthopedics, Faculty of Medicine 1, Charles University Prague, and Bulovka Hospital, Prague, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Dungl
authors.affiliation: Institute of Rheumatology, Prague, and Faculty of Medicine 1, Charles University Prague, Na Slupi 4, Prague, CZ 12850, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Pavelka
authors.affiliation: Institute of Rheumatology, Prague, and Faculty of Medicine 1, Charles University Prague, Na Slupi 4, Prague, CZ 12850, Czech Republic. stepan@revma.cz.
authors.forename: Jan J
authors.initials: JJ
authors.lastname: Stepan
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D019644:Arthroplasty, Replacement, Hip; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D015519:Bone Density; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005265:Femoral Neck Fractures / Q000097:blood* / Q000601:surgery; D005269:Femur / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000097:blood; D008297:Male; D008875:Middle Aged; D015207:Osteoarthritis, Hip / Q000097:blood*
chemical_list: D015415:Biomarkers; D001120:Arginine; C062187:pentosidine; D008239:Lysine
keywords: Biomarkers; Osteoarthritis; Osteoporosis; Pentosidine; Proximal femoral fracture