title: Elevated Plasma Level of Interferon-λ1 in Chronic Spontaneous Urticaria: Upregulated Expression in CD8(+) and Epithelial Cells and Induction of Inflammatory Cell Accumulation.
abstract: Interferon- (IFN-) λ1 is regarded as a potent bio-active molecule in innate immunity. However, little is known about its role in chronic spontaneous urticaria (CSU). We therefore investigated expression of IFN-λ1 in CSU, its cellular location, and its influence on inflammatory cell accumulation by using flow cytometry analysis, skin tissue dispersion, immunohistochemical stain, and a mouse peritoneal inflammation model. The results showed that level of IFN-λ1 was 2.0-fold higher in plasma of the patients with CSU than the level in healthy control (HC) subjects. Among leukocytes examined, only CD8(+) T cells expressed more IFN-λ1 in CSU blood. Double labeling immunohistochemical staining revealed that IFN-λ1(+) inflammatory cells such as mast cells, eosinophils, B cells, neutrophils, and macrophages were mainly located in dermis, whereas epidermis tissue highly expressed IFN-λ1. IFN-λ1 induced a dose-dependent increase in number of eosinophils, lymphocytes, mast cells, macrophages, and neutrophils in the peritoneum of mice at 6 h following injection, which was inhibited by pretreatment of the animals with anti-intercellular adhesion molecule- (ICAM-) 1 and/or anti-L-selectin antibodies. In conclusion, IFN-λ1 is likely to play a role in the pathogenesis of CSU. Blocking IFN-λ1 production may help to reduce the accumulation of inflammatory cells in the involved CSU skin.
authors.affiliation: Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China; Department of Dentistry, The Second Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: S F
authors.initials: SF
authors.lastname: Wang
authors.affiliation: Department of Dentistry, The Second Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Gao
authors.affiliation: Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Y N
authors.initials: YN
authors.lastname: Xu
authors.affiliation: Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: D N
authors.initials: DN
authors.lastname: Li
authors.affiliation: Allergy and Inflammation Research Institute, The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou 515031, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: S H
authors.identifier: 0000-0003-4760-4816
authors.initials: SH
authors.lastname: He
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D016207:Cytokines / Q000097:blood* / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007372:Interferons; D016753:Interleukin-10 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D007378:Interleukins / Q000097:blood*; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D014581:Urticaria / Q000097:blood*; D000094632:Thymic Stromal Lymphopoietin; D000095022:Interferon Lambda
chemical_list: D016207:Cytokines; D007371:Interferon-gamma; D007372:Interferons; D016753:Interleukin-10; D015847:Interleukin-4; D007378:Interleukins; D000094632:Thymic Stromal Lymphopoietin; C000624353:interferon-lambda, human; D000095022:Interferon Lambda; C511668:interferon-lambda protein, mouse