title: Steroidogenic enzymes of adipose tissue in modulation of trivalent chromium in a mouse model of PCOS.
abstract: Polycystic ovary syndrome (PCOS) is a type of endocrine metabolic disorder with many different consequences to health, most commonly infertility, obesity and insulin resistance. Trivalent chromium (Cr3+) was previously found to improve the metabolic profiles of patients with PCOS. The aim of this study was to explore the effect of Cr on regulating steroidogenic enzymes in adipose tissue. Female BALB/c mice were divided into three groups (n = 6 per group): the control group, PCOS + placebo milk group and PCOS + Cr-containing milk group. The dietary intake of Cr significantly decreased fasting blood sugar (FBS) and homeostasis model assessment of insulin resistance levels in the murine model of PCOS. Importantly, we found significant correlations among the levels of Cr, insulin and dehydroepiandrosterone (DHEA). In adipose tissue, decreases in the enzyme expressions of 3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-hydroxysteroid dehydrogenase, but not of aromatase, were observed. By understanding the role of steroidogenic enzymes in PCOS in normal and pathological states, trace elements may be used as a form of adjunctive therapy in the management of patients with PCOS.
authors.affiliation: a Department of Veterinary Medicine , College of Veterinary Medicine, National Chung Hsing University , Taichung , Taiwan.
authors.forename: Tsung-Sheng
authors.initials: TS
authors.lastname: Chen
authors.affiliation: a Department of Veterinary Medicine , College of Veterinary Medicine, National Chung Hsing University , Taichung , Taiwan.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Chen
authors.affiliation: a Department of Veterinary Medicine , College of Veterinary Medicine, National Chung Hsing University , Taichung , Taiwan.
authors.forename: Chia-Hsin
authors.initials: CH
authors.lastname: Liu
authors.affiliation: a Department of Veterinary Medicine , College of Veterinary Medicine, National Chung Hsing University , Taichung , Taiwan.
authors.forename: Chi-Ching
authors.initials: CC
authors.lastname: Sun
authors.affiliation: a Department of Veterinary Medicine , College of Veterinary Medicine, National Chung Hsing University , Taichung , Taiwan.
authors.forename: Frank Chiahung
authors.initials: FC
authors.lastname: Mao
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000187:drug effects / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001141:Aromatase / Q000187:drug effects / Q000378:metabolism*; D002857:Chromium / Q000008:administration & dosage / Q000494:pharmacology*; D003687:Dehydroepiandrosterone / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011085:Polycystic Ovary Syndrome / Q000188:drug therapy*; D014131:Trace Elements / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D014131:Trace Elements; D002857:Chromium; D003687:Dehydroepiandrosterone; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; D001141:Aromatase
keywords: Adipose tissue; dehydroepiandrosterone; polycystic ovary syndrome; steroidogenic enzymes; trivalent chromium