title: Proteomic analysis of the mature Brassica stigma reveals proteins with diverse roles in vegetative and reproductive development.
abstract: The stigma, the specialized apex of the Brassicaceae gynoecium, plays a role in pollen capture, discrimination, hydration, germination, and guidance. Despite this crucial role in reproduction, the global proteome underlying Brassicaceae stigma development and function remains largely unknown. As a contribution towards the characterization of the Brassicaceae dry stigma global proteome, more than 2500 Brassica napus mature stigma proteins were identified using three different gel-based proteomics approaches. Most stigma proteins participated in Metabolic Processes, Responses to Stimulus or Stress, Cellular or Developmental Processes, and Transport. The stigma was found to express a wide variety of proteins with demonstrated roles in cellular and organ development including proteins known to be involved in cellular expansion and morphogenesis, embryo development, as well as gynoecium and stigma development. Comparisons to a corresponding proteome from a very morphologically different Poaceae dry stigma showed a very similar distribution of proteins among different functional categories, but also revealed evident distinctions in protein composition especially in glucosinolate and carotenoid metabolism, photosynthesis, and self-incompatibility. To our knowledge, this study reports the largest Brassicaceae stigma protein dataset described to date.
authors.affiliation: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada; Carleton University, Department of Biology, 1125 Colonel By Drive, Ottawa, ON, Canada. Electronic address: NazilaNazemof@cmail.carleton.ca.
authors.forename: Nazila
authors.initials: N
authors.lastname: Nazemof
authors.affiliation: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada. Electronic address: Philippe.Couroux@agr.gc.ca.
authors.forename: Philippe
authors.initials: P
authors.lastname: Couroux
authors.affiliation: Carleton University, Department of Biology, 1125 Colonel By Drive, Ottawa, ON, Canada. Electronic address: Tim.Xing@carleton.ca.
authors.forename: Tim
authors.initials: T
authors.lastname: Xing
authors.affiliation: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada. Electronic address: Laurian.Robert@agr.gc.ca.
authors.forename: Laurian S
authors.initials: LS
authors.lastname: Robert
mesh_terms: D029688:Brassica napus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D035264:Flowers / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020543:Proteome; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Brassica napus; Electrophoresis; Mass spectrometry; Proteomics; Stigma; Triticale