title: Genomic diversity of theAvian leukosis virussubgroup Jgp85gene in different organs of an infected chicken.
abstract: The genomic diversity ofAvian leukosis virussubgroup J (ALV-J) was investigated in an experimentally infected chicken. ALV-J variants in tissues from four different organs of the same bird were re-isolated in DF-1 cells, and theirgp85gene was amplified and cloned. Ten clones from each organ were sequenced and compared with the original inoculum strain, NX0101. The minimum homology of each organ ranged from 96.7 to 97.6%, and the lowest homology between organs was only 94.9%, which was much lower than the 99.1% homology of inoculum NX0101, indicating high diversity of ALV-J, even within the same bird. Thegp85mutations from the left kidney, which contained tumors, and the right kidney, which was tumor-free, had higher non-synonymous to synonymous mutation ratios than those in the tumor-bearing liver and lungs. Additionally, the mutational sites ofgp85gene in the kidney were similar, and they differed from those in the liver and lung, implying that organ- or tissue-specific selective pressure had a greater influence on the evolution of ALV-J diversity. These results suggest that more ALV-J clones from different organs and tissues should be sequenced and compared to better understand viral evolution and molecular epidemiology in the field.
authors.affiliation: Shandong Agricultural University, Taian 271018, China.
authors.forename: Fanfeng
authors.initials: F
authors.lastname: Meng
authors.affiliation: Shandong Agricultural University, Taian 271018, China.|Beijing Dafaun Poultry Breeding Company Ltd., Beijing 10010, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: Shandong Agricultural University, Taian 271018, China.|Beijing Dafaun Poultry Breeding Company Ltd., Beijing 10010, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Fang
authors.affiliation: Beijing Dafaun Poultry Breeding Company Ltd., Beijing 10010, China.
authors.forename: Yalong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Beijing Dafaun Poultry Breeding Company Ltd., Beijing 10010, China.
authors.forename: Lilong
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Beijing Dafaun Poultry Breeding Company Ltd., Beijing 10010, China.
authors.forename: Guiju
authors.initials: G
authors.lastname: Xing
authors.affiliation: Beijing Dafaun Poultry Breeding Company Ltd., Beijing 10010, China.
authors.forename: Fu
authors.initials: F
authors.lastname: Tian
authors.affiliation: Beijing Dafaun Poultry Breeding Company Ltd., Beijing 10010, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Shandong Agricultural University, Taian 271018, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Dong
authors.affiliation: Shandong Agricultural University, Taian 271018, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Chang
authors.affiliation: Shandong Agricultural University, Taian 271018, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Shandong Agricultural University, Taian 271018, China.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Shandong Agricultural University, Taian 271018, China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000821:virology*; D001354:Avian Leukosis Virus / Q000235:genetics*; D002645:Chickens; D014644:Genetic Variation; D011201:Poultry Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D014764:Viral Proteins
keywords: Avian leukosis virus subgroup J; genomic diversity; glycoprotein 85; mutation