title: Neuronal uptake of serum albumin is associated with neuron damage during the development of epilepsy.
abstract: It is well established that brain blood barrier dysfunction following the onset of seizures may lead to serum albumin extravasation into the brain. However, the effect of albumin extravasation on the development of epilepsy is yet to be fully elucidated. Previous studies have predominantly focused on the effect of albumin absorption by astrocytes; however, the present study investigated the effects of neuronal uptake of albuminin vitroand in kainic acid-induced Sprague-Dawley rat models of temporal lobe epilepsy. In the present study, electroencephalogram recordings were conducted to record seizure onset, Nissl and Evans blue staining were used to detect neuronal damage and albumin extravasation, respectively, and double immunofluorescence was used to explore neuronal absorption of albumin. Cell counting was also conductedin vitroto determine whether albumin contributes to neuronal death. The results of the present study indicated that extravasated serum albumin was absorbed by neurons, and the neurons that had absorbed albumin died and were dissolved 28 days after seizure onsetin vivo. Furthermore, significant neuronal death was detected after albumin absorptionin vitroin a dose- and time-dependent manner. These results suggested that albumin may be absorbed by neurons following the onset of seizures. Furthermore, the results indicated that neuronal albumin uptake may be associated with neuronal damage and death in epileptic seizures. Therefore, attenuating albumin extravasation following epileptic seizures may reduce brain damage and slow the development of epilepsy.
authors.affiliation: Department of Neurology, Dalian Municipal Central Hospital Affiliated to Dalian Medical University, Dalian, Liaoning 11600, P.R. China.
authors.forename: Zanhua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: No. 2 VIP Ward, Dalian Municipal Central Hospital Affiliated to Dalian Medical University, Dalian, Liaoning 11600, P.R. China.
authors.forename: Jinjie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurology, Dalian Municipal Central Hospital Affiliated to Dalian Medical University, Dalian, Liaoning 11600, P.R. China.
authors.forename: Suping
authors.initials: S
authors.lastname: Wang
authors.affiliation: Surgical Intensive Care Unit, Dalian Municipal Central Hospital Affiliated to Dalian Medical University, Dalian, Liaoning 11600, P.R. China.
authors.forename: Sibo
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Neurology, Shanghai First People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Zhao
keywords: albumin; epilepsy; neuron; seizure