title: Bioplotting of a bioactive alginate dialdehyde-gelatin composite hydrogel containing bioactive glass nanoparticles.
abstract: Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs) were produced by biofabrication to obtain a grid-like highly-hydrated composite. The material could induce the deposition of an apatite layer upon immersion in a biological-like environment to sustain cell attachment and proliferation. Composites were formulated with different concentrations of BGNPs synthetized from a sol-gel route, namely 0.1% and 0.5% (w/v). Strontium doped BGNPs were also used. EDS analysis suggested that the BGNPs loading promoted the growth of bone-like apatite layer on the surface when the constructs were immersed in a simulated body fluid. Moreover, the composite constructs could incorporate with high efficiency ibuprofen as a drug model. Furthermore, the biofabrication process allowed the successful incorporation of MG-63 cells into the composite material. Cells were distributed homogeneously within the hydrogel composite, and no differences were found in cell viability between ADA-GEL and the composite constructs, proving that the addition of BGNPs did not influence cell fate. Overall, the composite material showed potential for future applications in bone tissue engineering.
authors.affiliation: 3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence of Tissue Engineering and Regenerative Medicine, Avepark-Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal. ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal. Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstr. 6, D-91058 Erlangen, Germany.
authors.forename: Álvaro J
authors.initials: ÁJ
authors.lastname: Leite
authors.forename: Bapi
authors.initials: B
authors.lastname: Sarker
authors.forename: Tobias
authors.initials: T
authors.lastname: Zehnder
authors.forename: Raquel
authors.initials: R
authors.lastname: Silva
authors.forename: João F
authors.initials: JF
authors.lastname: Mano
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry; D001672:Biocompatible Materials; D062028:Bioprinting; D002460:Cell Line; D017076:Computer-Aided Design; D065546:Drug Liberation; D005780:Gelatin / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D007052:Ibuprofen / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010006:Osteoblasts
chemical_list: D000464:Alginates; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001672:Biocompatible Materials; D020100:Hydrogels; D005780:Gelatin; D007052:Ibuprofen