title: Occurrence of Toxoplasma gondii in domestic rabbits of Northeastern Brazil.
abstract: The present study aimed to conduct a serosurvey of toxoplasmosis in domestic rabbits of Northeastern Brazil. Blood samples and tissue fragments (brain, heart and diaphragm) were collected from 150 and 54 rabbits from the state of Pernambuco, Brazil, respectively. The serum samples were subjected to serological analysis (Modified Agglutination Test - MAT) and the tissue samples were assessed by PCR and histopathological analysis. Data collected through questionnaires were subjected to analysis of risk factors. According to the MAT and the PCR results, 6.7% (10/150; CI 3.2%-11.9%) of the rabbits were positive for anti-T. gondii antibodies and 9.25% (5/54) of the tissue fragments were positive for T. gondii DNA, respectively. Lesions associated with T. gondii infection, mainly characterized by granuloma, mononuclear cell infiltrates, degeneration areas and necrosis in brain and heart, were detected in the histopathological analysis. The risk factors associated with T. gondii infection identified in the present study were homemade food (odds ratio = 39.00) and contact between cats and rabbits (odds ratio = 52.00). This is the first report of toxoplasmosis in rabbits of Northeastern Brazil. The management problems identified in the present study must be corrected to reduce the frequency of positive animals in herds of rabbits.
authors.forename: Débora Costa Viegas
authors.initials: DC
authors.lastname: de Lima
authors.forename: André de Souza
authors.initials: Ade S
authors.lastname: Santos
authors.forename: Luana Thamires Rapôso
authors.initials: LT
authors.lastname: da Silva
authors.forename: Renata Pimentel Bandeira
authors.initials: RP
authors.lastname: de Melo
authors.forename: José Givanildo
authors.initials: JG
authors.lastname: da Silva
authors.forename: José Wilton Pinheiro
authors.initials: JW
authors.lastname: Júnior
authors.forename: Rinaldo Aparecido
authors.initials: RA
authors.lastname: Mota
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000097:blood / Q000469:parasitology; D000913:Antibodies, Protozoan / Q000097:blood; D001938:Brazil; D011817:Rabbits / Q000097:blood / Q000469:parasitology*; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000097:blood / Q000469:parasitology*
chemical_list: D000913:Antibodies, Protozoan