title: Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells.
abstract: OBJECTIVE
  Faulty wound healing is a global healthcare problem. Chronic wounds are generally characterized by a reduction in availability of growth factors. New strategies are being developed to deliver growth factors more effectively.
  
  
  METHODS
  In this study, we introduced electrospun scaffolds composed of polycaprolactone (PCL) nanofibers functionalized with adhered platelets, as a source of numerous growth factors. Three concentrations of platelets were immobilized to nanofibrous scaffolds by simple adhesion, and their influence on adhesion, proliferation and metabolic activity of seeded cells (murine fibroblasts, keratinocytes and melanocytes) was investigated.
  
  
  RESULTS
  The data obtained indicated that presence of platelets significantly promoted cell spreading, proliferation and metabolic activity in all the skin-associated cell types. There were no significant differences among tested concentrations of platelets, thus even the lowest concentration sufficiently promoted proliferation of the seeded cells.
  
  
  CONCLUSIONS
  Such complex stimulation is needed for improved healing of chronic wounds. However, the nanofibrous system can be used not only as a skin cover, but also in broader applications in regenerative medicine.
authors.affiliation: Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, 150 06, Prague 5, Czech Republic. karolina_vocetkova@labdemo.cz.|Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, 142 20, Prague 4, Czech Republic. karolina_vocetkova@labdemo.cz.|University Center for Energy Efficient Buildings, Czech Technical University in Prague, 273 43, Bustehrad, Czech Republic. karolina_vocetkova@labdemo.cz.|Faculty of Biomedical Engineering, Czech Technical University in Prague, 272 01, Kladno 2, Czech Republic. karolina_vocetkova@labdemo.cz.
authors.forename: K
authors.initials: K
authors.lastname: Vocetkova
authors.affiliation: Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, 150 06, Prague 5, Czech Republic.|Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, 142 20, Prague 4, Czech Republic.|University Center for Energy Efficient Buildings, Czech Technical University in Prague, 273 43, Bustehrad, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Buzgo
authors.affiliation: Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, 150 06, Prague 5, Czech Republic.|Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, 142 20, Prague 4, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Sovkova
authors.affiliation: Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, 150 06, Prague 5, Czech Republic.|Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, 142 20, Prague 4, Czech Republic.
authors.forename: D
authors.initials: D
authors.lastname: Bezdekova
authors.affiliation: Faculty of Biomedical Engineering, Czech Technical University in Prague, 272 01, Kladno 2, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Kneppo
authors.affiliation: Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, 150 06, Prague 5, Czech Republic.|Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, 142 20, Prague 4, Czech Republic.|University Center for Energy Efficient Buildings, Czech Technical University in Prague, 273 43, Bustehrad, Czech Republic.
authors.forename: E
authors.initials: E
authors.lastname: Amler
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology*; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D008544:Melanocytes / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D011091:Polyesters; C016240:polycaprolactone