title: [Preparation and in vitro drug release behavior of a novel matrine-loading chitosan/glycerol film].
abstract: OBJECTIVE
  To prepare a drug-loading film using chitosan and carboxymethyl chitosan as the carrier materials for delivering matrine to oral ulcers.
  
  
  METHODS
  Matrine-loading films using chitosan or carboxymethyl chitosan as the carrier materials were prepared by solution casting method and orthogonal experiment at room temperature. The mechanical properties, surface morphology and drug-loading capacity of the drug-loading film were characterized using tensile test, scanning electron microscopy (SEM), swelling test and in vitro drug release test.
  
  
  RESULTS
  When the molecular weight of chitosan was 650 000 and the mass ratio of chitosan/glycerol was 1:1.4, the prepared film had the maximum mechanical strength and tensile modulus reaching 0.7875 MPa. SEM observation showed that matrine aggregated at the bottom of the drug-loading film with an asymmetrical distribution. The in vitro drug release test showed that the film had a high drug-loading capacity and a sustained drug release property. The duration of drug release from the drug-loading film was prolonged as the molecular weight of chitosan increased, reaching 23 h when the molecular weight of chitosan was 650 000. The duration of drug release was further increased to 108 h when the bottom of the drug-loading film was coated with a layer of 1% carboxymethyl chitosan.
  
  
  CONCLUSIONS
  The matrix materials of the drug-loading film are natural, green, nontoxic and biodegradable, and the preparation of the film is simple without using large quantities of organic solvents. The novel drug-loading film can obviously prolong the duration of drugs release for better local drug delivery to oral ulcers in a sustained manner.
authors.affiliation: First Affiliated Hospital,Jinan University,Guangzhou 510632,China.E-mail: zclsir@126.com.
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Zhang
authors.forename: Tian-Jin
authors.initials: TJ
authors.lastname: Yang
authors.forename: Hua-Xin
authors.initials: HX
authors.lastname: Rao
authors.forename: Zi-Yong
authors.initials: ZY
authors.lastname: Zhang
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005990:Glycerol / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011807:Quinolizines / Q000737:chemistry*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D003692:Delayed-Action Preparations; D011807:Quinolizines; C514968:carboxymethyl-chitosan; D048271:Chitosan; D005990:Glycerol; D000093842:Matrines