title: Effects of testosterone on the expression levels of AMH, VEGF and HIF-1α in mouse granulosa cells.
abstract: The present study aimed to investigate the effects of testosterone on mouse granulosa cell morphology, and the expression levels of anti-Müllerian hormone (AMH), vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α (HIF-1α). Mouse granulosa cells were isolated and identified, and their morphology was examined using hematoxylin and eosin, F-actin, and follicle-stimulating hormone receptor staining. The mRNA expression levels of AMH, VEGF and HIF-1α were examined using reverse transcription-quantitative polymerase chain reaction, and their protein secretion levels were investigated using enzyme-linked immunosorbent assays. Testosterone treatment did not affect granulosa cell morphology; however, it significantly increased the mRNA expression levels of AMH and VEGF, and the protein secretion levels of AMH, VEGF and HIF-1α. These results suggested that testosterone was able to regulate the functions of granulosa cells by upregulating the expression levels of AMH, VEGF and HIF-1α.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China; International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200030, P.R. China; Institute of Embryo-Fetal Original Adult Disease, Shanghai Jiao Tong University, School of Medicine, Shanghai 200030, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China.
authors.forename: Shan-Feng
authors.initials: SF
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China.
authors.forename: Jin-Dan
authors.initials: JD
authors.lastname: Zheng
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China; Department of Obstetrics and Gynecology, The Second Affiliated Hospital (Jiande Branch), Medical School of Zhejiang University, The First People's Hospital of Jiande, Hangzhou, Zhejiang 311600, P.R. China.
authors.forename: Chun-Bo
authors.initials: CB
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China.
authors.forename: Yi-Nan
authors.initials: YN
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Liu
authors.affiliation: Department of Key Laboratory of Tissue Engineering, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Huang
keywords: anti-Müllerian hormone; hypoxia-inducible factor-1α; testosterone; vascular endothelial growth factor