title: Overexpression of KIF11 in Gastric Cancer with Intestinal Mucin Phenotype.
abstract: OBJECTIVE
  Gastric cancer (GC) is one of the most common human cancers. A useful method of gastric cancer stem cell (CSC) characterization is spheroid colony formation. Previously, we reported that KIF11 expression is >2-fold in spheroid-body-forming GC cells compared with parental cells. Here, we analyzed the expression and distribution of KIF11 in human GC by immunohistochemistry.
  
  
  METHODS
  Expression of KIF11 in 165 GC cases was determined using immunohistochemistry. For mucin phenotypic expression analysis of GC, immunostaining of MUC5AC, MUC6, MUC2 and CD10 was evaluated. RNA interference was used to inhibit KIF11 expression in GC cell lines.
  
  
  RESULTS
  In total, 119 of 165 GC cases (72%) were positive for KIF11. Expression of KIF11 was not associated with any clinicopathologic characteristics; however, it was observed frequently in GC exhibiting an intestinal phenotype. Both the number and size of spheres formed by MKN-74 cells were significantly reduced following transfection of KIF11-targeting siRNA compared with negative-control siRNA. Furthermore, levels of phosphorylated Erk1/2 were lower in KIF11 siRNA-transfected cells than with negative-control siRNA-transfected cells.
  
  
  CONCLUSIONS
  These results indicate that KIF11 is involved in intestinal mucin phenotype GC.
authors.affiliation: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.
authors.forename: Takeharu
authors.initials: T
authors.lastname: Imai
authors.forename: Naohide
authors.initials: N
authors.lastname: Oue
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nishioka
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Mukai
authors.forename: Takashi
authors.initials: T
authors.lastname: Oshima
authors.forename: Naoya
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sentani
authors.forename: Keisuke
authors.initials: K
authors.lastname: Matsusaki
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yoshida
authors.forename: Wataru
authors.initials: W
authors.lastname: Yasui
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D016547:Kinesins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009077:Mucins / Q000378:metabolism*; D010641:Phenotype; D012189:Retrospective Studies; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000601:surgery
chemical_list: D014408:Biomarkers, Tumor; C470243:KIF11 protein, human; D009077:Mucins; D016547:Kinesins