title: Research Techniques Made Simple: Methodology and Clinical Applications of RNA Sequencing.
abstract: RNA sequencing is a method of transcriptome profiling that utilizes next-generation sequencing technology. It offers several distinct advantages over hybridization-based approaches, most notably superior sensitivity and the capacity for de novo transcript discovery. This article describes RNA sequencing methodology, summarizes important technological advances and challenges, and discusses applications for this technique in the field of dermatology.
authors.affiliation: Department of Dermatology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA. Electronic address: whitleysk@upmc.edu.
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Whitley
authors.affiliation: Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.
authors.forename: William T
authors.initials: WT
authors.lastname: Horne
authors.affiliation: Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA; Richard King Mellon Institute for Pediatric Research, Pittsburgh, Pennsylvania, USA.
authors.forename: Jay K
authors.initials: JK
authors.lastname: Kolls
mesh_terms: D001706:Biopsy; D003880:Dermatology / Q000379:methods*; D005091:Exons; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D015894:Genome, Human; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D009154:Mutation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D020033:Protein Isoforms; D017423:Sequence Analysis, RNA / Q000379:methods*; D012867:Skin / Q000378:metabolism; D059467:Transcriptome
chemical_list: D020033:Protein Isoforms