title: Isolation of a monoclonal antibody that recognizes the origin binding domain of JCV, but not SV40, large T-antigen.
abstract: Within immunocompromised populations, the JC polyomavirus is the cause of the often-fatal disease Progressive Multifocal Leukoencephalopathy (PML). JC virus encodes a protein, termed T-antigen (T-ag), which is essential for its replication and pathogenicity. Previous studies of JCV T-ag have, in general, used antibodies raised against SV40 T-ag. Unfortunately, SV40 T-ag is also detected in humans and therefore there have been concerns about cross-reactivity. To address this issue, we have isolated a monoclonal antibody that binds to the JCV, but not the SV40, T-ag origin-binding domain (OBD). Furthermore, the region on the surface of the JCV T-ag OBD that is recognized by the "anti-JCV OBD mAb" has been mapped. We also demonstrate that the "anti-JCV OBD mAb" will be a useful reagent for standard techniques (e.g., Westerns blots and ELISAs). Finally, we note that additional monoclonal Abs that are specific for the T-ags encoded by the other human polyomaviruses could be generated by adopting the approach described herein.
authors.affiliation: Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine, Boston, 02111 MA, USA; Cell Essentials Inc., 75 Kneeland Street, Boston, MA 02111, USA.
authors.forename: Shelley A
authors.initials: SA
authors.lastname: Grubman
authors.affiliation: Sackler Institute of Graduate Biomedical Medicine, New York University School of Medicine, 550 First Avenue, New York, NY 10016-6481, USA.
authors.forename: Jong
authors.initials: J
authors.lastname: Shin
authors.affiliation: Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, 02111 MA, USA.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Phelan
authors.affiliation: Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, 02111 MA, USA.
authors.forename: Aaron
authors.initials: A
authors.lastname: Gong
authors.affiliation: Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, 02111 MA, USA.
authors.forename: Hande
authors.initials: H
authors.lastname: Can
authors.affiliation: Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, 02111 MA, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Dilworth
authors.affiliation: Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, 02111 MA, USA.
authors.forename: Sandeep Kuntadi
authors.initials: SK
authors.lastname: Kini
authors.affiliation: Institut de Recherches Cliniques de Montreal (IRCM), 110 Pine Avenue West, Montreal, Quebec, Canada H2W 1R7; Department of Biochemistry and Molecular Medicine Universite de Montreal Montreal, Quebec, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Gagnon
authors.affiliation: Institut de Recherches Cliniques de Montreal (IRCM), 110 Pine Avenue West, Montreal, Quebec, Canada H2W 1R7; Department of Biochemistry and Molecular Medicine Universite de Montreal Montreal, Quebec, Canada.
authors.forename: Jacques
authors.initials: J
authors.lastname: Archambault
authors.affiliation: Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, 02111 MA, USA.
authors.forename: Gretchen
authors.initials: G
authors.lastname: Meinke
authors.affiliation: Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, 02111 MA, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Bohm
authors.affiliation: Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine, Boston, 02111 MA, USA.
authors.forename: Douglas M
authors.initials: DM
authors.lastname: Jefferson
authors.affiliation: Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, 02111 MA, USA. Electronic address: Peter.Bullock@tufts.edu.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Bullock
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D000957:Antigens, Viral, Tumor / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D001665:Binding Sites; D003429:Cross Reactions / Q000276:immunology; D018604:Epitope Mapping; D007577:JC Virus / Q000276:immunology*; D051379:Mice; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs
chemical_list: D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor
keywords: JCV; Monoclonal antibody; Polyomavirus