title: Determination of Rodenticide Tetramethylenedisulfotetramine (Tetramine) in Processed Foods by Gas Chromatography-Tandem Mass Spectrometry.
abstract: A GC-MS/MS method for determination of the rodenticide tetramethylenedisulfotetramine was developed. Tetramethylenedisulfotetramine was extracted from the sample with ethyl acetate in the presence of anhydrous sodium sulfate. Then, an aliquot of the extract was evaporated under vacuum, followed by acetonitrile/hexane partitioning, and cleanup with a tandem graphitized carbon/primary secondary amine (PSA) column, prior to GC-MS/MS analysis. The recoveries from 10 processed foods, all of which were fortified at 0.1 mg/kg, were in the range of 85-96%, and the relative standard deviations were less than 7%. The proposed method effectively removed co-extracted matrix components, and matrix effects were negligible in the GC-MS/MS analysis. In addition, no interfering peaks were found in the chromatograms of the blank samples at the retention time of tetramethylenedisulfotetramine, indicating that the method is highly selective. Overall results suggest that the proposed method is suitable for determining tetramethylenedisulfotetramine contained in processed foods.
authors.affiliation: National Institute of Health Sciences.
authors.forename: Shizuka
authors.initials: S
authors.lastname: Saito-Shida
authors.forename: Satoru
authors.initials: S
authors.lastname: Nemoto
authors.forename: Reiko
authors.initials: R
authors.lastname: Teshima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
mesh_terms: D000085:Acetates; D000097:Acetonitriles; D000588:Amines; D001952:Bridged-Ring Compounds / Q000032:analysis* / Q000302:isolation & purification; D002244:Carbon; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005511:Food Handling; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006108:Graphite; D006586:Hexanes; D012378:Rodenticides / Q000032:analysis* / Q000302:isolation & purification; D013431:Sulfates; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000085:Acetates; D000097:Acetonitriles; D000588:Amines; D001952:Bridged-Ring Compounds; D006586:Hexanes; D012378:Rodenticides; D013431:Sulfates; C012036:sodium sulfate; D002244:Carbon; C007650:ethyl acetate; D006108:Graphite; C010349:tetramethylenedisulfotetramine; C032159:acetonitrile