title: Thirty-Second Net Stressor Task in Adult Zebrafish.
abstract: Zebrafish have become a popular animal model for behavioral neuroscience (Gerlai, 2014). Recent studies have demonstrated that brief experimental handling prior to euthanizing animals can subsequently alter biological measures quantified post-mortem (e.g.cortisol levels) (Ramsayet al., 2009; Tranet al., 2014). Here we provide a detailed protocol for a simple 30-sec net stressor task for adult zebrafish that increases whole-body cortisol levels without altering the levels of whole-brain dopamine, 3, 4-dihydroxyphenylacetic acid, serotonin, and 5-hydroxyindoleacetic acid (Tranet al., 2014).
authors.affiliation: Department of Cell and Systems Biology, University of Toronto Mississauga, Mississauga, Canada;
authors.forename: Steven
authors.initials: S
authors.lastname: Tran
authors.affiliation: Department of Cell and Systems Biology, University of Toronto Mississauga, Mississauga, Canada;; Department of Psychology, University of Toronto Mississauga, Mississauga, Canada.
authors.forename: Robert
authors.initials: R
authors.lastname: Gerlai