title: Female Mice are Resistant to Fabp1 Gene Ablation-Induced Alterations in Brain Endocannabinoid Levels.
abstract: Although liver fatty acid binding protein (FABP1, L-FABP) is not detectable in the brain, Fabp1 gene ablation (LKO) markedly increases endocannabinoids (EC) in brains of male mice. Since the brain EC system of females differs significantly from that of males, it was important to determine if LKO differently impacted the brain EC system. LKO did not alter brain levels of arachidonic acid (ARA)-containing EC, i.e. arachidonoylethanolamide (AEA) and 2-arachidonoylglycerol (2-AG), but decreased non-ARA-containing N-acylethanolamides (OEA, PEA) and 2-oleoylglycerol (2-OG) that potentiate the actions of AEA and 2-AG. These changes in brain potentiating EC levels were not associated with: (1) a net decrease in levels of brain membrane proteins associated with fatty acid uptake and EC synthesis; (2) a net increase in brain protein levels of cytosolic EC chaperones and enzymes in EC degradation; or (3) increased brain protein levels of EC receptors (CB1, TRVP1). Instead, the reduced or opposite responsiveness of female brain EC levels to loss of FABP1 (LKO) correlated with intrinsically lower FABP1 level in livers of WT females than males. These data show that female mouse brain endocannabinoid levels were unchanged (AEA, 2-AG) or decreased (OEA, PEA, 2-OG) by complete loss of FABP1 (LKO).
authors.affiliation: Department of Physiology and Pharmacology, Texas A&M University, 4466 TAMU, College Station, TX, 77843-4466, USA.
authors.forename: Gregory G
authors.initials: GG
authors.lastname: Martin
authors.affiliation: Department of Pathobiology, Texas A&M University, College Station, TX, 77843-4466, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Chung
authors.affiliation: Department of Pathobiology, Texas A&M University, College Station, TX, 77843-4466, USA.
authors.forename: Danilo
authors.initials: D
authors.lastname: Landrock
authors.affiliation: Department of Physiology and Pharmacology, Texas A&M University, 4466 TAMU, College Station, TX, 77843-4466, USA.
authors.forename: Kerstin K
authors.initials: KK
authors.lastname: Landrock
authors.affiliation: Protein Chemistry Laboratory, Texas A&M University, College Station, TX, 77843-2128, USA.
authors.forename: Lawrence J
authors.initials: LJ
authors.lastname: Dangott
authors.affiliation: Department of Anesthesiology, Stony Brook University, Stony Brook, NY, 11794, USA.
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Anesthesiology, Stony Brook University, Stony Brook, NY, 11794, USA.
authors.forename: Martin
authors.initials: M
authors.lastname: Kaczocha
authors.affiliation: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, 58202-9037, USA.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Murphy
authors.affiliation: Department of Pathobiology, Texas A&M University, College Station, TX, 77843-4466, USA.
authors.forename: Ann B
authors.initials: AB
authors.lastname: Kier
authors.affiliation: Department of Physiology and Pharmacology, Texas A&M University, 4466 TAMU, College Station, TX, 77843-4466, USA. fschroeder@cvm.tamu.edu.
authors.forename: Friedhelm
authors.initials: F
authors.lastname: Schroeder
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D063388:Endocannabinoids / Q000378:metabolism*; D004983:Ethanolamines / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000172:deficiency*; D005260:Female; D005989:Glycerides / Q000378:metabolism; D008297:Male; D051379:Mice
chemical_list: D063388:Endocannabinoids; D004983:Ethanolamines; C493320:Fabp1 protein, mouse; D050556:Fatty Acid-Binding Proteins; D005989:Glycerides; C022203:N-acylethanolamines; D016718:Arachidonic Acid; C505247:2-oleoylglycerol
keywords: Brain; Endocannabinoid; FABP1; Female; Gene ablation; Mouse