title: A label-free electrochemical immunosensor for the detection of cardiac marker using graphene quantum dots (GQDs).
abstract: A label-free immunosensor based on electrochemical impedance spectroscopy has been developed for the sensitive detection of a cardiac biomarker myoglobin (cMyo). Hydrothermally synthesized graphene quantum dots (GQDs) have been used as an immobilized template on screen printed electrodes for the construction of an impedimetric sensor platform. The GQDs-modified electrode was conjugated with highly specific anti-myoglobin antibodies to develop the desired immunosensor. The values of charge transfer resistance (Rct) were monitored as a function of varying antigen concentration. The Rct value of the immunosensor showed a linear increase (from 0.20 to 0.31kΩ) in the range of 0.01-100ng/mL cMyo. The specific detection of cMyo was also made in the presence of other competing proteins. The limit of detection for the proposed immunosensor was estimated as 0.01ng/mL which is comparable to the standard ELISA techniques.
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30-C, Chandigarh, 160030, India; Academy of Scientific and Innovative Research, CSIR-CSIO, Sector 30-C, Chandigarh, 160030, India; CSIR-Institute of Microbial Technology (CSIR-IMTECH), Sector 39, Chandigarh, 160038, India.
authors.forename: Satish K
authors.initials: SK
authors.lastname: Tuteja
authors.affiliation: Department of Chemistry, Hanyang University, Seoul, 133-791, Republic of Korea.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30-C, Chandigarh, 160030, India; Academy of Scientific and Innovative Research, CSIR-CSIO, Sector 30-C, Chandigarh, 160030, India.
authors.forename: Manil
authors.initials: M
authors.lastname: Kukkar
authors.affiliation: Department of Chemistry, Hanyang University, Seoul, 133-791, Republic of Korea.
authors.forename: Chung Kil
authors.initials: CK
authors.lastname: Song
authors.affiliation: CSIR-Institute of Microbial Technology (CSIR-IMTECH), Sector 39, Chandigarh, 160038, India.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Mutreja
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30-C, Chandigarh, 160030, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Singh
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30-C, Chandigarh, 160030, India.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Paul
authors.affiliation: Department of Chemistry, Hanyang University, Seoul, 133-791, Republic of Korea.
authors.forename: Haiwon
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Civil Engineering & Environmental Engineering, Hanyang University, Seoul, 133-791, Republic of Korea.
authors.forename: Ki-Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30-C, Chandigarh, 160030, India; Academy of Scientific and Innovative Research, CSIR-CSIO, Sector 30-C, Chandigarh, 160030, India. Electronic address: dr.akashdeep@csio.res.in.
authors.forename: Akash
authors.initials: A
authors.lastname: Deep
authors.affiliation: CSIR-Institute of Microbial Technology (CSIR-IMTECH), Sector 39, Chandigarh, 160038, India. Electronic address: raman@imtech.res.in.
authors.forename: C Raman
authors.initials: CR
authors.lastname: Suri
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D058266:Dielectric Spectroscopy / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D009211:Myoglobin / Q000032:analysis / Q000097:blood*; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D055910:Antibodies, Immobilized; D009211:Myoglobin; D006108:Graphite
keywords: Cardiac marker; Electrochemical Impedance Spectroscopy; Graphene quantum dots; Label-free immunosensor; Myoglobin