title: Synthesis and Characterization of a Poly(amido amine) Modified Magnetic Nanocarrier for Controlled Delivery of Doxorubicin.
abstract: We prepared a magnetic poly(amido amine) (G2.5)-hydrazine hydrate nanocarrier, denoted by MNPs@PAMAM-H. PAMAM dendrimer was conjugated onto the surface of magnetic nanoparticles (MNPs) to increase the biocompatibility of the nanocarrier and provided a large number of reactive sites for coupling of drug molecules. DOX was covalently attached to the nanocarrier via a pH-sensitive linker, hydrazone bond, which hydrolyzes in the acidic lysosomal environment to allow pH-sensitive release of DOX. DOX was successfully loaded into the nanocarrier with a high drug loading (27.53%) and entrapment efficiency (86.44%). Nearly 88% DOX was released within 60 h at pH 5.0, compared with only 30% at pH 7.4. The in vitro MTT assay in HeLa cells demonstrated that MNPs@PAMAM-DOX exhibited high anti-tumor activity, while the MNPs@PAMAM-H were practically non-toxic. These results revealed that MNPs@ PAMAM-H were biocompatible. The Synthesized nanocarrier had the potential to be used as MR probe and guide DOX to enter target sites in cancer therapy by an outer magnet.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Xu
authors.forename: Juan
authors.initials: J
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Renbing
authors.initials: R
authors.lastname: Tian
mesh_terms: D003692:Delayed-Action Preparations / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D050091:Dendrimers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004317:Doxorubicin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D003692:Delayed-Action Preparations; D050091:Dendrimers; C104700:PAMAM Starburst; C531249:Poly(amidoamine); D011073:Polyamines; D004317:Doxorubicin