title: Construction of Tissue-Engineered Nerve Conduits Seeded with Neurons Derived from Hair-Follicle Neural Crest Stem Cells.
abstract: Tissue-engineered nerve conduits are widely used for the study of peripheral nerve injury repair. With regard to repairing long nerve defects, stem-cell-derived neurons are recommended as seed cells. As hair-follicle neural crest stem cells (hfNCSCs) are easily to be harvested from patients and have the potential to differentiate into neuronal cells, hfNCSCs-derived neurons are an ideal candidate choice. Acellular nerve grafts, a type of biological material scaffold, with intact collagen structure, with biocompatibility and less toxicity are obtained through removing live cells with 1 % lysolecithin, are also an ideal choice. In the present report, we describe a tissue-engineered nerve conduit seeded with rat hfNCSCs-derived neurons into the beagle acellular sciatic nerve scaffold. Our goal is to provide a novel engineered therapeutic for repairing peripheral nerve injury with long distance defects.
authors.affiliation: Department of Anatomy, Institute of Biomedical Engineering, Second Military Medical University, Shanghai, People's Republic of China. liu_fang403@163.com.|AntiCancer, Inc., San Diego, CA, USA. liu_fang403@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Anatomy, Institute of Biomedical Engineering, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Lin
authors.affiliation: Department of Anatomy, Institute of Biomedical Engineering, Second Military Medical University, Shanghai, People's Republic of China. chuansen@yahoo.com.
authors.forename: Chuansen
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018859:Hair Follicle / Q000166:cytology*; D009416:Nerve Regeneration; D009432:Neural Crest / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D059348:Peripheral Nerve Injuries / Q000628:therapy; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds
keywords: Acellular nerve scaffold; Hair follicle; Nerve injury repair; Neural crest stem cells; Neuron; Tissue-engineered nerve conduit