title: Protein-stabilizing and cell-penetrating properties of α-helix domain of 30Kc19 protein.
abstract: The protein-stabilizing and cell-penetrating activities of Bombyx mori 30Kc19 α-helix domain (30Kc19α) are investigated. Recently, 30Kc19 protein has been studied extensively as it has both protein-stabilizing and cell-penetrating properties. However, it is unknown which part of 30Kc19 is responsible for those properties. 30Kc19 protein is composed of two distinct domains, an α-helix N-terminal domain (30Kc19α) and a β-trefoil C-terminal domain (30Kc19β). The authors construct and produce truncated forms of 30Kc19 to demonstrate their biological functions. Interestingly, 30Kc19α was shown to be responsible for both the protein-stabilizing and cell-penetrating properties of 30Kc19 protein. 30Kc19α shows even higher protein delivery activity than did whole 30Kc19 protein and has low cytotoxicity when added to cell culture medium. Therefore, based on its multifunctional properties, 30Kc19α can be developed as a novel candidate for a therapeutic protein carrier into various cells and tissues.
authors.affiliation: Interdisciplinary Program for Bioengineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jina
authors.initials: J
authors.lastname: Ryu
authors.affiliation: Interdisciplinary Program for Bioengineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hyoju
authors.initials: H
authors.lastname: Kim
authors.affiliation: The School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hee Ho
authors.initials: HH
authors.lastname: Park
authors.affiliation: The School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hong Jai
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Ju Hyun
authors.initials: JH
authors.lastname: Park
authors.affiliation: Division of Bioengineering, Incheon National University, Incheon, Republic of Korea.
authors.forename: Won Jong
authors.initials: WJ
authors.lastname: Rhee
authors.affiliation: Interdisciplinary Program for Bioengineering, Seoul National University, Seoul, Republic of Korea.|The School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.|Advanced Institutes of Convergence Technology, Suwon, Republic of Korea.
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Park
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry / Q000235:genetics*; D057846:Cell-Penetrating Peptides / Q000737:chemistry* / Q000235:genetics; D016503:Drug Delivery Systems; D005786:Gene Expression Regulation; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics / Q000627:therapeutic use; D000072756:Protein Conformation, alpha-Helical; D000072417:Protein Domains; D055550:Protein Stability
chemical_list: D057846:Cell-Penetrating Peptides; D019476:Insect Proteins
keywords: 30Kc19; 30Kc19α; Biomaterials; Drug delivery; Protein stability