title: Cell-Based Screening: Cellular Assays with a Molecular Endpoint Measured by SAMDI Mass Spectrometry (Small 28/2016).
abstract: On page 3811, M. Mrksich and co-workers culture cells using self-assembled monolayers presenting cell adhesion ligands and enzyme substrates. A lysis buffer disrupts the cell membranes, releasing enzymes that modify the immobilized substrates. These modifications can be measured with SAMDI mass spectrometry, giving a high-throughput, cell-based assay.
authors.affiliation: Department of Biomedical Engineering, Department of Chemistry, Northwestern University, Evanston, IL, 60208, USA.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Berns
authors.affiliation: Department of Biomedical Engineering, Department of Chemistry, Northwestern University, Evanston, IL, 60208, USA.
authors.forename: Maria D
authors.initials: MD
authors.lastname: Cabezas
authors.affiliation: Department of Biomedical Engineering, Department of Chemistry, Northwestern University, Evanston, IL, 60208, USA.|Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA.
authors.forename: Milan
authors.initials: M
authors.lastname: Mrksich
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
keywords: analytical methods; bioassays; high-content screening; self-assembled monolayers