title: Xenopus Oocyte As a Model System to Study Store-Operated Ca(2+) Entry (SOCE).
abstract: Store-operated Ca(2+) entry (SOCE) is a ubiquitous Ca(2+) influx pathway at the cell membrane that is regulated by Ca(2+) content in intracellular stores. SOCE is important for a multitude of physiological processes, including muscle development, T-cell activation, and fertilization. Therefore, understanding the molecular regulation of SOCE is imperative. SOCE activation requires conformational and spatial changes in proteins located in both the endoplasmic reticulum and plasma membrane. This leads to the generation of an ionic current of very small amplitude. Both biochemical and electrophysiological parameters of SOCE can be difficult to record in small mammalian cells. In this protocol we present the different methodologies that enable the study of SOCE in a unique model system, the frog oocyte, which provides several advantages and have contributed significantly to our understanding of SOCE regulation.
authors.affiliation: Department of Physiology and Biophysics, Weill Cornell Medicine Qatar, Education City, Qatar Foundation Doha, Qatar.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Courjaret
authors.affiliation: Department of Physiology and Biophysics, Weill Cornell Medicine Qatar, Education City, Qatar Foundation Doha, Qatar.
authors.forename: Khaled
authors.initials: K
authors.lastname: Machaca
keywords: RNA injection; Xenopus oocyte; confocal imaging; meiosis; store-operated Ca2+ entry (SOCE); two-electrode voltage clamp