title: Real-time selective sequencing using nanopore technology.
abstract: The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set.
authors.affiliation: School of Life Sciences, University of Nottingham, Nottingham, UK.
authors.forename: Matthew
authors.identifier: 0000-0002-5264-0929
authors.initials: M
authors.lastname: Loose
authors.affiliation: School of Life Sciences, University of Nottingham, Nottingham, UK.
authors.forename: Sunir
authors.initials: S
authors.lastname: Malla
authors.affiliation: School of Life Sciences, University of Nottingham, Nottingham, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Stout
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D001330:Electronic Data Processing; D015723:Gene Library; D015894:Genome, Human; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D058608:Nanopores; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA / Q000379:methods*; D013997:Time Factors