title: Generation of bioactive peptide hydrolysates from cattle plasma using plant and fungal proteases.
abstract: Four protease preparations from plant and fungal sources (papain, bromelain, FP400 and FPII) were used to hydrolyse plasma which was separated from slaughterhouse cattle blood. The o-phthaldialdehyde assay was used to follow the release of TCA-soluble peptides over a 24h period. Hydrolysis profiles were displayed using SDS-PAGE. The in vitro antioxidant and antimicrobial activities of the hydrolysates were determined. The results showed that hydrolysates of cattle plasma generated with fungal protease FPII had higher antioxidant activities. Overall than hydrolysates generated with papain, bromelain and FP400. None of the hydrolysates demonstrated antimicrobial activity. The FPII peptide hydrolysate was fractionated using gel permeation chromatography, OFFGEL isoelectric focusing and RP-HPLC. The RP-HPLC fraction with highest antioxidant activity contained 15 novel peptide sequences. The use of protease FPII to hydrolyse cattle plasma resulted in a hydrolysate with high antioxidant properties and unique peptide sequences.
authors.affiliation: Department of Food Science, University of Otago, Dunedin, New Zealand.
authors.forename: Clara S F
authors.initials: CSF
authors.lastname: Bah
authors.affiliation: Department of Food Science, University of Otago, Dunedin, New Zealand. Electronic address: aladin.bekhit@otago.ac.nz.
authors.forename: Alaa El-Din A
authors.initials: AEA
authors.lastname: Bekhit
authors.affiliation: Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: McConnell
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin, New Zealand.
authors.forename: Alan
authors.initials: A
authors.lastname: Carne
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002417:Cattle / Q000097:blood*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005609:Free Radicals / Q000032:analysis / Q000737:chemistry; D005658:Fungi / Q000201:enzymology*; D006868:Hydrolysis; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*; D011492:Protein Hydrolysates / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005609:Free Radicals; D010446:Peptide Fragments; D011492:Protein Hydrolysates; D010447:Peptide Hydrolases
keywords: Antioxidant activity; Cattle blood plasma; Fungal protease; Hydrolysis; Peptide sequence; Plant protease