title: Metagenomics-Guided Mining of Commercially Useful Biocatalysts from Marine Microorganisms.
abstract: Marine microorganisms are a rich reservoir of highly diverse and unique biocatalysts that offer potential applications in food, pharmaceutical, fuel, and cosmetic industries. The fact that only less than 1% of microbes in any marine habitats can be cultured under standard laboratory conditions has hampered access to their extraordinary biocatalytic potential. Metagenomics has recently emerged as a powerful and well-established tool to investigate the vast majority of hidden uncultured microbial diversity for the discovery of novel industrially relevant enzymes from different types of environmental samples, such as seawater, marine sediment, and symbiotic microbial consortia. We discuss here in this review about approaches and methods in metagenomics that have been used and can potentially be used to mine commercially useful biocatalysts from uncultured marine microbes.
authors.affiliation: Research and Development Center for Marine and Fisheries Product Processing and Biotechnology, Central Jakarta, Indonesia. Electronic address: agustinus.uria@gmail.com.
authors.forename: A R
authors.initials: AR
authors.lastname: Uria
authors.affiliation: Research and Development Center for Marine and Fisheries Product Processing and Biotechnology, Central Jakarta, Indonesia.
authors.forename: D S
authors.initials: DS
authors.lastname: Zilda
mesh_terms: D059001:Aquatic Organisms / Q000201:enzymology / Q000382:microbiology*; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000302:isolation & purification; D017753:Ecosystem; D004798:Enzymes / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015723:Gene Library; D019015:Geologic Sediments / Q000382:microbiology; D056186:Metagenomics; D012623:Seawater / Q000382:microbiology; D017385:Sequence Homology
chemical_list: D004269:DNA, Bacterial; D004798:Enzymes
keywords: Biocatalysts; Functional and homology screenings; Marine microorganisms; Metagenomics