title: Alterations of filopodia by near infrared photoimmunotherapy: evaluation with 3D low-coherent quantitative phase microscopy.
abstract: Filopodia are highly organized cellular membrane structures that facilitate intercellular communication. Near infrared photoimmunotherapy (NIR-PIT) is a newly developed cancer treatment that causes necrotic cell death. Three-dimensional low-coherent quantitative phase microscopy (3D LC-QPM) is based on a newly established low-coherent interference microscope designed to obtain serial topographic images of the cellular membrane. Herein, we report rapid involution of filopodia after NIR-PIT using 3D LC-QPM. For 3T3/HER2 cells, the number of filopodia decreased immediately after treatment with significant differences. Volume and relative height of 3T3/HER2 cells increased immediately after NIR light exposure, but significant differences were not observed. Thus, disappearance of filopodia, evaluated by 3D LC-QPM, is an early indicator of cell membrane damage after NIR-PIT.
authors.affiliation: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
authors.forename: Tadanobu
authors.initials: T
authors.lastname: Nagaya
authors.affiliation: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Sato
authors.affiliation: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
authors.forename: Toshiko
authors.initials: T
authors.lastname: Harada
authors.affiliation: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Okuyama
authors.affiliation: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
authors.forename: Peter L
authors.initials: PL
authors.lastname: Choyke
authors.affiliation: Central Research Laboratory, Hamamatsu Photonics K.K., 5000, Hirakuchi, Hamakita-ku, Hamamatsu 434-8601, Japan.
authors.forename: Toyohiko
authors.initials: T
authors.lastname: Yamauchi
authors.affiliation: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
authors.forename: Hisataka
authors.initials: H
authors.lastname: Kobayashi
keywords: (110.6880) Three-dimensional image acquisition; (110.6915) Time imaging; (170.3880) Medical and biological imaging