title: Application of a cell microarray chip system for accurate, highly sensitive, and rapid diagnosis for malaria in Uganda.
abstract: Accurate, sensitive, rapid, and easy operative diagnosis is necessary to prevent the spread of malaria. A cell microarray chip system including a push column for the recovery of erythrocytes and a fluorescence detector was employed for malaria diagnosis in Uganda. The chip with 20,944 microchambers (105 μm width and 50 μm depth) was made of polystyrene. For the analysis, 6 μl of whole blood was employed, and leukocytes were practically removed by filtration through SiO2-nano-fibers in a column. Regular formation of an erythrocyte monolayer in each microchamber was observed following dispersion of an erythrocyte suspension in a nuclear staining dye, SYTO 21, onto the chip surface and washing. About 500,000 erythrocytes were analyzed in a total of 4675 microchambers, and malaria parasite-infected erythrocytes could be detected in 5 min by using the fluorescence detector. The percentage of infected erythrocytes in each of 41 patients was determined. Accurate and quantitative detection of the parasites could be performed. A good correlation between examinations via optical microscopy and by our chip system was demonstrated over the parasitemia range of 0.0039-2.3438% by linear regression analysis (R(2) = 0.9945). Thus, we showed the potential of this chip system for the diagnosis of malaria.
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-cho 2217-14, Takamatsu 761-0395, Japan.
authors.forename: Shouki
authors.initials: S
authors.lastname: Yatsushiro
authors.affiliation: Panasonic Co., Automotive &Industrial Systems Company, 1006 Ooaza-Kadoma, Kadoma, Osaka 571-8506, Japan.
authors.forename: Takeki
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-cho 2217-14, Takamatsu 761-0395, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Yamamura
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-cho 2217-14, Takamatsu 761-0395, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Abe
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-cho 2217-14, Takamatsu 761-0395, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Obana
authors.affiliation: Panasonic Co., Automotive &Industrial Systems Company, 1006 Ooaza-Kadoma, Kadoma, Osaka 571-8506, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nogami
authors.affiliation: Panasonic Co., Automotive &Industrial Systems Company, 1006 Ooaza-Kadoma, Kadoma, Osaka 571-8506, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Panasonic Co., Automotive &Industrial Systems Company, 1006 Ooaza-Kadoma, Kadoma, Osaka 571-8506, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sesei
authors.affiliation: Panasonic Co., Automotive &Industrial Systems Company, 1006 Ooaza-Kadoma, Kadoma, Osaka 571-8506, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Oka
authors.affiliation: Faculty of Science, Gulu University, P.O. Box 166 Gulu, Uganda.
authors.forename: Joseph
authors.initials: J
authors.lastname: Okello-Onen
authors.affiliation: Faculty of Medicine, Gulu University, P.O. Box 166 Gulu, Uganda.
authors.forename: Emmanuel I
authors.initials: EI
authors.lastname: Odongo-Aginya
authors.affiliation: St. Mary's Hospital Lacor, P.O. Box 180 Gulu, Uganda.
authors.forename: Mary Auma
authors.initials: MA
authors.lastname: Alai
authors.affiliation: Faculty of Medicine, Gulu University, P.O. Box 166 Gulu, Uganda.
authors.forename: Alex
authors.initials: A
authors.lastname: Olia
authors.affiliation: Faculty of Medicine, Gulu University, P.O. Box 166 Gulu, Uganda.
authors.forename: Dennis
authors.initials: D
authors.lastname: Anywar
authors.affiliation: Department of International Affairs and Tropical Medicine, Tokyo Women's Medical University, School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Sakurai
authors.affiliation: Department of Molecular Protozoology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
authors.forename: Nirianne Mq
authors.initials: NM
authors.lastname: Palacpac
authors.affiliation: Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Mita
authors.affiliation: Department of Molecular Protozoology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Horii
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering and Nagoya University, Furo-cho, Nagoya 464-8603, Japan.|MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University, Furo-cho, Nagoya 464-8603, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-cho 2217-14, Takamatsu 761-0395, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kataoka
mesh_terms: D004912:Erythrocytes / Q000469:parasitology; D005453:Fluorescence; D006801:Humans; D007962:Leukocytes / Q000469:parasitology; D008288:Malaria / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology*; D008853:Microscopy / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D018512:Parasitemia / Q000097:blood / Q000175:diagnosis / Q000469:parasitology; D010963:Plasmodium falciparum / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods; D014454:Uganda
chemical_list: D011137:Polystyrenes; D012822:Silicon Dioxide