title: Molecular detection and in vitro antioxidant activity of S-allyl-L-cysteine (SAC) extracted from Allium sativum.
abstract: It is well known that Allium sativum has potential applications to clinical treatment of various cancers due to its remarkable ability in eliminating free radicals and increasing metabolism. An allyl-substituted cysteine derivative - S-allyl-L-cysteine (SAC) was separated and identified from Allium sativum. The extracted SAC was reacted with 1-pyrenemethanol to obtain pyrene-labelled SAC (Py-SAC) to give SAC fluorescence properties. Molecular detection of Py-SAC was conducted by steady-state fluorescence spectroscopy and time-resolved fluorescence method to quantitatively measure concentrations of Py-SAC solutions. The ability of removing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical using Py-SAC was determined through oxygen radical absorbance capacity (ORAC). Results showed the activity of Py-SAC and Vitamin C (VC) with ORAC as index, the concentrations of Py-SAC and VC were 58.43 mg/L and 5.72 mg/L respectively to scavenge DPPH, and 8.16 mg/L and 1.67 mg/L to scavenge •OH respectively. Compared with VC, the clearance rates of Py-SAC to scavenge DPPH were much higher, Py-SAC could inhibit hydroxyl radical. The ability of removing radical showed a dose-dependent relationship within the scope of the drug concentration.
authors.affiliation: Xuzhou Institute of Technology College of Food Engineering XuZhou China.
authors.forename: Y-E
authors.initials: YE
authors.lastname: Sun
authors.affiliation: Xuzhou Institute of Technology College of Food Engineering XuZhou China weidong_wang_xit@sina.com.
authors.forename: W-D
authors.initials: WD
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D003545:Cysteine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D005737:Garlic / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011721:Pyrenes; D017665:Hydroxyl Radical; C065299:S-allylcysteine; C030984:pyrene; C004931:1,1-diphenyl-2-picrylhydrazyl; D003545:Cysteine; D001205:Ascorbic Acid