title: A Magnetic Nanoparticle Based Nucleic Acid Isolation and Purification Instrument for DNA Extraction of Escherichia Coli O157: H7.
abstract: The objective of this study was to evaluate the performance of a nucleic acid isolation and purification instrument using Escherichia coli O157:H7 as the model. The instrument was developed with magnetic nanoparticles for efficiently capturing nucleic acids and an intelligent mechanical unit for automatically performing the whole nucleic acid extraction process. A commercial DNA extraction kit from Huier Nano Company was used as reference. Nucleic acids in 1 ml of E. coli O157: H7 at a concentration of 5 x 10(8) CFU/mL were extracted by using this instrument and the kit in parallel and then detected by an ultraviolet spectrophotometer to obtain A260 values and A260/A280 values for the determination of the extracted DNA's quantity and purity, respectively. The A260 values for the instrument and the kit were 0.78 and 0.61, respectively, and the A260/A280 values were 1.98 and 1.93. The coefficient of variations of these parallel tests ranged from 10.5% to 16.7%. The results indicated that this nucleic acid isolation and purification instrument could extract a comparable level of nucleic acid within 50 min compared to the commercial DNA extraction kit.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Chen
authors.forename: Jianhan
authors.initials: J
authors.lastname: Lin
authors.forename: Qin
authors.initials: Q
authors.lastname: Jiang
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Shengjun
authors.initials: S
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D002138:Calibration; D004269:DNA, Bacterial / Q000302:isolation & purification*; D019453:Escherichia coli O157 / Q000235:genetics*; D008280:Magnetics; D053758:Nanoparticles; D009696:Nucleic Acids / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004269:DNA, Bacterial; D009696:Nucleic Acids