title: Snapshot hyperspectral retinal imaging using compact spectral resolving detector array.
abstract: Hyperspectral retinal imaging captures the light spectrum from each imaging pixel. It provides spectrally encoded retinal physiological and morphological information, which could potentially benefit diagnosis and therapeutic monitoring of retinal diseases. The key challenges in hyperspectral retinal imaging are how to achieve snapshot imaging to avoid motions between the images from multiple spectral bands, and how to design a compact snapshot imager suitable for clinical use. Here, we developed a compact, snapshot hyperspectral fundus camera for rodents using a novel spectral resolving detector array (SRDA), on which a thin-film Fabry-Perot cavity filter was monolithically fabricated on each imaging pixel. We achieved hyperspectral retinal imaging with 16 wavelength bands (460 to 630 nm) at 20 fps. We also demonstrated false-color vessel contrast enhancement and retinal oxygen saturation (sO2) measurement through spectral analysis. This work could potentially bring hyperspectral retinal imaging from bench to bedside.
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Biqin
authors.initials: B
authors.lastname: Dong
authors.affiliation: Department of Ophthalmology, Northwestern University, Chicago, IL 60611, USA.
authors.forename: Joel V
authors.initials: JV
authors.lastname: Kaluzny
authors.affiliation: Department of Ophthalmology, Northwestern University, Chicago, IL 60611, USA.
authors.forename: Amani A
authors.initials: AA
authors.lastname: Fawzi
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA.|Department of Ophthalmology, Northwestern University, Chicago, IL 60611, USA.
authors.forename: Hao F
authors.initials: HF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004867:Equipment Design; D005654:Fundus Oculi; D006801:Humans; D008297:Male; D010100:Oxygen / Q000032:analysis*; D020318:Rats, Long-Evans; D012160:Retina / Q000000981:diagnostic imaging*; D013057:Spectrum Analysis
chemical_list: D010100:Oxygen
keywords: hyperspectral imaging; retinal imaging; retinal oxygen saturation