title: Novel role for the midbody in primary ciliogenesis by polarized epithelial cells.
abstract: The primary cilium is a membrane protrusion that is crucial for vertebrate tissue homeostasis and development. Here, we investigated the uncharacterized process of primary ciliogenesis in polarized epithelial cells. We show that after cytokinesis, the midbody is inherited by one of the daughter cells as a remnant that initially locates peripherally at the apical surface of one of the daughter cells. The remnant then moves along the apical surface and, once proximal to the centrosome at the center of the apical surface, enables cilium formation. The physical removal of the remnant greatly impairs ciliogenesis. We developed a probabilistic cell population-based model that reproduces the experimental data. In addition, our model explains, solely in terms of cell area constraints, the various observed transitions of the midbody, the beginning of ciliogenesis, and the accumulation of ciliated cells. Our findings reveal a biological mechanism that links the three microtubule-based organelles-the midbody, the centrosome, and the cilium-in the same cellular process.
authors.affiliation: Department of Cell Biology and Immunology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Bernabé-Rubio
authors.affiliation: Electron Microscopy Unit, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Germán
authors.identifier: 0000-0003-0265-5409
authors.initials: G
authors.lastname: Andrés
authors.affiliation: Department of Cell Biology and Immunology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Javier
authors.identifier: 0000-0003-3147-5730
authors.initials: J
authors.lastname: Casares-Arias
authors.affiliation: Department of Cell Biology and Immunology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Fernández-Barrera
authors.affiliation: Department of Cell Biology and Immunology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Laura
authors.identifier: 0000-0003-4011-577X
authors.initials: L
authors.lastname: Rangel
authors.affiliation: Department of Cell Biology and Immunology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Reglero-Real
authors.affiliation: Cell Biology and Neurobiology Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
authors.forename: David C
authors.identifier: 0000-0002-0602-210X
authors.initials: DC
authors.lastname: Gershlick
authors.affiliation: Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.
authors.forename: José J
authors.initials: JJ
authors.lastname: Fernández
authors.affiliation: Department of Cell Biology and Immunology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Millán
authors.affiliation: Department of Cell Biology and Immunology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Correas
authors.affiliation: Department of Condensed Matter Physics, Instituto de Ciencias de Materiales Nicolás Cabrera and Instituto de Física de la Materia Condensada, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: David G
authors.initials: DG
authors.lastname: Miguez
authors.affiliation: Department of Cell Biology and Immunology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain maalonso@cbm.csic.es.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Alonso
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D016764:Cell Polarity; D002470:Cell Survival; D018385:Centrosome / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism* / Q000648:ultrastructure; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D021621:Imaging, Three-Dimensional; D061985:Madin Darby Canine Kidney Cells; D018715:Microscopy, Video; D008871:Microvilli / Q000378:metabolism; D008938:Mitosis; D008954:Models, Biological; D059010:Single-Cell Analysis; D020691:rab GTP-Binding Proteins / Q000378:metabolism
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D020691:rab GTP-Binding Proteins