title: Immunogenicity of recombinantLactobacillus plantarumNC8 expressing goose parvovirus VP2 gene in BALB/c mice.
abstract: Goose parvovirus (GPV) continues to be a threat to goose farms and has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection. In our study,Lactobacillus (L.) plantarumNC8 was selected as a vector to express the VP2 gene of GPV, and recombinantL. plantarumpSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2 × 109colony-forming unit/200 μL dose of the recombinantL. plantarumstrain, and the ratios and numbers of CD11c+, CD3+CD4+, CD3+CD8+, and interferon gamma- and tumor necrosis factor alpha-expressing spleen lymphocytes in the pSIP409-VP2/NC8 group were higher than those in the control groups. In addition, we assessed the capacity ofL. plantarumSIP409-VP2/NC8 to induce secretory IgA production. We conclude that administered pSIP409-VP2/NC8 leads to relatively extensive cellular responses. This study provides information on GPV infection and offers a clear framework of options available for GPV control strategies.
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Chun-Wei
authors.initials: CW
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Fang-Yu
authors.initials: FY
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Yan-Long
authors.initials: YL
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Jing-Tao
authors.initials: JT
authors.lastname: Hu
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China.|Shandong Baolai-leelai Bioengineering Co. Ltd, Taian 271000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Gui-Lian
authors.initials: GL
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Wang
mesh_terms: D000818:Animals; D005260:Female; D005814:Genes, Viral / Q000235:genetics; D007111:Immunity, Cellular / Q000276:immunology; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000276:immunology* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D030781:Organisms, Genetically Modified; D010322:Parvoviridae Infections / Q000517:prevention & control / Q000662:veterinary; D019219:Parvovirinae / Q000235:genetics*; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology / Q000494:pharmacology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology / Q000494:pharmacology
chemical_list: D007071:Immunoglobulin A, Secretory; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines
keywords: Lactobacillus plantarum; VP2 gene; goose parvovirus; immunization