title: The association of novel IL-33 polymorphisms with sIL-33 and risk of systemic lupus erythematosus.
abstract: The serum level of IL-33 was upregulated in systemic lupus erythematosus (SLE), which may be used as biomarkers and/or therapeutic targets for SLE. The aim of this study was to investigate the association of four novel polymorphisms of IL-33 with risk of SLE. The study population comprised 540 Chinese individuals, including 257subjects with SLE and 283 healthy controls. The gene polymorphism was measured using Snapshot SNP genotyping assays and confirmed by sequencing. Serum IL-33 (sIL-33) levels were measured by ELISA. The serum SLE levels were significantly higher in the group of patients with SLE than those in the control group (P<0.001). The rs1891385C allele was associated with a significantly increased risk of SLE as compared with the rs1891385 A allele (OR=1.405, 95% CI, 1.052-1.875, P=0.021). The C-T-T-G haplotype was significantly increased the risk of SLE (OR=1.411; 95% CI, 1.021-1.948; P=0.036). IL-33 gene rs1891385 polymorphism was significantly associated with the expression of sIL-33 in the SLE patients. Besides, in our study, we found that the unregulated sIL-33 level was significantly associated with the abnormal changed CRP and ESR. This is the first study reporting the IL-33 gene polymorphisms and SLE, which may help refine the SLE risk profile.
authors.affiliation: Department of Dermatology, Affiliated Hospital of Youjiang Medical University for Nationalities, China. Electronic address: gjnsdy-1987@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.affiliation: Youjiang Medical University for Nationalities, China. Electronic address: 15807861838@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Department of Clinical Laboratory, Affiliated Hospital of Youjiang Medical University for Nationalities, China. Electronic address: 373606322@qq.com.
authors.forename: You-Fan
authors.initials: YF
authors.lastname: Peng
authors.affiliation: Department of Clinical Laboratory, Affiliated Hospital of Youjiang Medical University for Nationalities, China. Electronic address: hhtzyy99@126.com.
authors.forename: Hua-Tuo
authors.initials: HT
authors.lastname: Huang
authors.affiliation: Department of Dermatology, Affiliated Hospital of Youjiang Medical University for Nationalities, China. Electronic address: yylanyan@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lan
authors.affiliation: Department of Clinical Laboratory, Affiliated Hospital of Youjiang Medical University for Nationalities, China. Electronic address: GXresearcher123@163.com.
authors.forename: Ye-Sheng
authors.initials: YS
authors.lastname: Wei
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D001799:Blood Sedimentation; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D000067596:Interleukin-33 / Q000097:blood / Q000235:genetics*; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D055815:Young Adult
chemical_list: C506536:IL33 protein, human; D000067596:Interleukin-33
keywords: Gene; IL-33; Inflammation; Polymorphism; Systemic lupus erythematosus