title: Circadian RNA expression elicited by 3'-UTR IRAlu-paraspeckle associated elements.
abstract: Paraspeckles are nuclear bodies form around the long non-coding RNA, Neat1, and RNA-binding proteins. While their role is not fully understood, they are believed to control gene expression at a post-transcriptional level by means of the nuclear retention of mRNA containing in their 3'-UTR inverted repeats of Alu sequences (IRAlu). In this study, we found that, in pituitary cells, all components of paraspeckles including four major proteins and Neat1 displayed a circadian expression pattern. Furthermore the insertion of IRAlu at the 3'-UTR of the EGFP cDNA led to a rhythmic circadian nuclear retention of the egfp mRNA that was lost when paraspeckles were disrupted whereas insertion of a single antisense Alu had only a weak effect. Using real-time video-microscopy, these IRAlu were further shown to drive a circadian expression of EGFP protein. This study shows that paraspeckles, thanks to their circadian expression, control circadian gene expression at a post-transcriptional level.
authors.affiliation: Faculté de Médecine Nord, Aix Marseille Université, CNRS, CRN2M-UMR7286, Marseille, France.
authors.forename: Manon
authors.initials: M
authors.lastname: Torres
authors.affiliation: Faculté de Médecine Nord, Aix Marseille Université, CNRS, CRN2M-UMR7286, Marseille, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Becquet
authors.affiliation: Faculté de Médecine Nord, Aix Marseille Université, CNRS, CRN2M-UMR7286, Marseille, France.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Blanchard
authors.affiliation: Faculté de Médecine Nord, Aix Marseille Université, CNRS, CRN2M-UMR7286, Marseille, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Guillen
authors.affiliation: Faculté de Médecine Nord, Aix Marseille Université, CNRS, CRN2M-UMR7286, Marseille, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Boyer
authors.affiliation: Faculté de Médecine Nord, Aix Marseille Université, CNRS, CRN2M-UMR7286, Marseille, France.
authors.forename: Mathias
authors.initials: M
authors.lastname: Moreno
authors.affiliation: Faculté de Médecine Nord, Aix Marseille Université, CNRS, CRN2M-UMR7286, Marseille, France.
authors.forename: Jean-Louis
authors.identifier: 0000-0002-2900-5468
authors.initials: JL
authors.lastname: Franc
authors.affiliation: Faculté de Médecine Nord, Aix Marseille Université, CNRS, CRN2M-UMR7286, Marseille, France.
authors.forename: Anne-Marie
authors.identifier: 0000-0002-3278-4642
authors.initials: AM
authors.lastname: François-Bellan
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D002940:Circadian Rhythm; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D000069416:Intravital Microscopy; D055029:Inverted Repeat Sequences; D018715:Microscopy, Video; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats
chemical_list: D020413:3' Untranslated Regions; C577276:NEAT1 long non-coding RNA, human; D009687:Nuclear Proteins; D062085:RNA, Long Noncoding; D016601:RNA-Binding Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: cell biology; chromosomes; circadian oscillators; circadian rhythm; genes; nuclear RNA retention; paraspeckle; pituitary cells