title: Exogenous paraoxonase-1 during oocyte maturation improves bovine embryo development in vitro.
abstract: Paraoxonase-1 (PON1) is an enzyme found in serum and follicular fluid that protects cell membrane and circulating lipids against oxidative damage. The aims of this study were to measure the direct effects of recombinant PON1 (rPON1) on bovine oocyte maturation at the molecular level (gene expression) and to measure the carry-over effects of PON1 on pre-implantation embryo development in vitro. COCs were submitted to IVM with the addition of 0.0, 0.02, 0.04 and 0.08 mg ml(-1) of rPON1, corresponding to an average PON1 arylesterase enzyme activity of 2.2 ± 0.4, 15.5 ± 1.5, 30.2 ± 3.0 and 57.9 ± 5.0 U ml(-1) , respectively. The results indicated that addition of rPON1 during IVM improved embryo development in a dose-dependent manner as D7 embryo development was 22.2%, 29.4%, 32.2% and 37.0% for the treatment groups, respectively (p = 0.02). In conclusion, addition of PON1 enzyme during IVM exerted dose-related positive effects on embryo development rates to blastocysts.
authors.affiliation: Department of Veterinary Clinics, Veterinary College, Universidade Federal de Pelotas, Pelotas, Brazil.
authors.forename: Jaa
authors.initials: J
authors.lastname: Rincón
authors.affiliation: Department of Pathology, Veterinary College, Universidade Federal de Pelotas, Pelotas, Brazil.
authors.forename: E M
authors.initials: EM
authors.lastname: Madeira
authors.affiliation: Department of Pathology, Veterinary College, Universidade Federal de Pelotas, Pelotas, Brazil.
authors.forename: F T
authors.initials: FT
authors.lastname: Campos
authors.affiliation: Department of Pathology, Veterinary College, Universidade Federal de Pelotas, Pelotas, Brazil.
authors.forename: B
authors.initials: B
authors.lastname: Mion
authors.affiliation: Department of Pathology, Veterinary College, Universidade Federal de Pelotas, Pelotas, Brazil.
authors.forename: J F
authors.initials: JF
authors.lastname: Silva
authors.affiliation: Department of Clinical Studies Section of Reproduction and Behavior, School of Veterinary Medicine, University of Pennsylvania New Bolton Center, Kennett Square, PA, USA.
authors.forename: V A
authors.initials: VA
authors.lastname: Absalón-Medina
authors.affiliation: Department of Animal Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, USA.
authors.forename: W R
authors.initials: WR
authors.lastname: Butler
authors.affiliation: Department of Veterinary Clinics, Veterinary College, Universidade Federal de Pelotas, Pelotas, Brazil.
authors.forename: M N
authors.initials: MN
authors.lastname: Corrêa
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Clima Temperado, Pelotas, Brazil.
authors.forename: Lmc
authors.initials: L
authors.lastname: Pegoraro
authors.affiliation: Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, Brazil. augusto.schneider@ufpel.edu.br.
authors.forename: A
authors.initials: A
authors.lastname: Schneider
mesh_terms: D000818:Animals; D043303:Aryldialkylphosphatase / Q000494:pharmacology*; D002417:Cattle / Q000196:embryology*; D003470:Culture Media; D054885:Cumulus Cells / Q000187:drug effects; D046149:Embryo Culture Techniques / Q000662:veterinary*; D004622:Embryo, Mammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary*
chemical_list: D003470:Culture Media; D043303:Aryldialkylphosphatase