title: Fabrication of a turbid optofluidic phantom device with tunable μa and μ's to simulate cutaneous vascular perfusion.
abstract: Microfluidic devices are oftenly used to calibrate the imaging reconstruction, because they simulate the morphology of microvasculature. However, for lack of optical properties in microfluidics, the functional recovery of oximetry information cannot be verified. In this work, we describe the fabrication of a novel turbid optofluidic tissue phantom. It is designed to mimic the vascular perfusion and the turbid nature of cutaneous tissue. This phantom contains an interior hollow microfluidic structure with a diameter of ϕave = 50 μm. The microfluidic structure includes the geometry of an inlet, a river-like assay and an outlet. This structure can be perfused by hemoglobin solution to mimic the cutaneous micro-circulation. The multiple-layered phantom matrices exhibit the representative optical parameters of human skin cutis, namely the absorption coefficient μa and the reduced scattering coefficient . The geometry of the generated microfluidic structure is investigated by using Spectral-Domain Optical Coherence Tomography. This optofluidic phantom bridges the gap between tissue equivalent phantoms and Lab-On-Chip devices. Perspectively, this device can be used to calibrate a variety of optical angiographic imaging approaches.
authors.affiliation: Chair of Photonic Technologies, Friedrich-Alexander Universität Erlangen-Nürnberg, Konrad-Zuse-Str. 3/5, D-91052 Erlangen, Germany.|Erlangen Graduate School in Advanced Optical Technologies, Paul-Gordan-Str. 6, D-91052 Erlangen, Germany.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Chair of Photonic Technologies, Friedrich-Alexander Universität Erlangen-Nürnberg, Konrad-Zuse-Str. 3/5, D-91052 Erlangen, Germany.|Erlangen Graduate School in Advanced Optical Technologies, Paul-Gordan-Str. 6, D-91052 Erlangen, Germany.
authors.forename: Midhat
authors.initials: M
authors.lastname: Ahmed
authors.affiliation: Chair of Photonic Technologies, Friedrich-Alexander Universität Erlangen-Nürnberg, Konrad-Zuse-Str. 3/5, D-91052 Erlangen, Germany.|Erlangen Graduate School in Advanced Optical Technologies, Paul-Gordan-Str. 6, D-91052 Erlangen, Germany.
authors.forename: Tom
authors.initials: T
authors.lastname: Häfner
authors.affiliation: Chair of Photonic Technologies, Friedrich-Alexander Universität Erlangen-Nürnberg, Konrad-Zuse-Str. 3/5, D-91052 Erlangen, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Klämpfl
authors.affiliation: Erlangen Graduate School in Advanced Optical Technologies, Paul-Gordan-Str. 6, D-91052 Erlangen, Germany.|Department of Oral and Maxillofacial Surgery, Friedrich-Alexander Universität Erlangen-Nürnberg, Glückstr. 11, D-91054 Erlangen, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Stelzle
authors.affiliation: Chair of Photonic Technologies, Friedrich-Alexander Universität Erlangen-Nürnberg, Konrad-Zuse-Str. 3/5, D-91052 Erlangen, Germany.|Erlangen Graduate School in Advanced Optical Technologies, Paul-Gordan-Str. 6, D-91052 Erlangen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Schmidt
mesh_terms: D056656:Lab-On-A-Chip Devices; D055806:Microvessels; D041623:Tomography, Optical Coherence