title: Alteration of Neutrophil Reactive Oxygen Species Production by Extracts of Devil's Claw (Harpagophytum).
abstract: Harpagophytum, Devil's Claw, is a genus of tuberiferous xerophytic plants native to southern Africa. Some of the taxa are appreciated for their medicinal effects and have been traditionally used to relieve symptoms of inflammation. The objectives of this pilot study were to investigate the antioxidant capacity and the content of total phenols, verbascoside, isoverbascoside, and selected iridoids, as well as to investigate the capacity of various Harpagophytum taxa in suppressing respiratory burst in terms of reactive oxygen species produced by human neutrophils challenged with phorbol myristate acetate (PMA), opsonised Staphylococcus aureus, and Fusobacterium nucleatum. Harpagophytum plants were classified into different taxa according to morphology, and DNA analysis was used to confirm the classification. A putative new variety of H. procumbens showed the highest degree of antioxidative capacity. Using PMA, three Harpagophytum taxa showed anti-inflammatory effects with regard to the PBS control. A putative hybrid between H. procumbens and H. zeyheri in contrast showed proinflammatory effect on the response of neutrophils to F. nucleatum in comparison with treatment with vehicle control. Harpagophytum taxa were biochemically very variable and the response in suppressing respiratory burst differed. Further studies with larger number of subjects are needed to corroborate anti-inflammatory effects of different taxa of Harpagophytum.
authors.affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Balsgård, Fjälkestadsvägen 459, 291 94 Kristianstad, Sweden; Biological Sciences, University of Botswana, Private Bag UB 00704, Gaborone, Botswana.
authors.forename: Mbaki
authors.initials: M
authors.lastname: Muzila
authors.affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Balsgård, Fjälkestadsvägen 459, 291 94 Kristianstad, Sweden.
authors.forename: Kimmo
authors.identifier: 0000-0003-3229-5010
authors.initials: K
authors.lastname: Rumpunen
authors.affiliation: School of Dentistry and MRC Centre for Immune Regulation, University of Birmingham, Saint Chads Queensway, Birmingham B4 7ET, UK.
authors.forename: Helen
authors.initials: H
authors.lastname: Wright
authors.affiliation: School of Dentistry and MRC Centre for Immune Regulation, University of Birmingham, Saint Chads Queensway, Birmingham B4 7ET, UK.
authors.forename: Helen
authors.initials: H
authors.lastname: Roberts
authors.affiliation: School of Dentistry and MRC Centre for Immune Regulation, University of Birmingham, Saint Chads Queensway, Birmingham B4 7ET, UK.
authors.forename: Melissa
authors.initials: M
authors.lastname: Grant
authors.affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Balsgård, Fjälkestadsvägen 459, 291 94 Kristianstad, Sweden.
authors.forename: Hilde
authors.initials: H
authors.lastname: Nybom
authors.affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Balsgård, Fjälkestadsvägen 459, 291 94 Kristianstad, Sweden.
authors.forename: Jasna
authors.initials: J
authors.lastname: Sehic
authors.affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Balsgård, Fjälkestadsvägen 459, 291 94 Kristianstad, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Ekholm
authors.affiliation: School of Health and Society, University of Kristianstad, 291 88 Kristianstad, Sweden.
authors.forename: Cecilia
authors.identifier: 0000-0001-8602-6524
authors.initials: C
authors.lastname: Widén
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis; D001205:Ascorbic Acid / Q000494:pharmacology; D044822:Biodiversity; D001902:Botswana; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005843:Geography; D005960:Glucosides / Q000494:pharmacology; D031684:Harpagophytum / Q000737:chemistry*; D006801:Humans; D039823:Iridoids / Q000494:pharmacology; D007501:Iron / Q000378:metabolism; D008165:Luminol / Q000378:metabolism; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D010949:Plasma / Q000378:metabolism; D025341:Principal Component Analysis; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D005960:Glucosides; D039823:Iridoids; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C064683:isoacteoside; D008165:Luminol; D007501:Iron; D001205:Ascorbic Acid