title: MiRNA-101 inhibits oral squamous-cell carcinoma growth and metastasis by targeting zinc finger E-box binding homeobox 1.
abstract: MicroRNAs (miRNAs) are implicated in the pathogenesis of oral squamous-cell carcinoma (OSCC). miR-101 is involved in the development and progression of OSCC, but the biological functions and underlying molecular mechanisms of this miRNA remain largely unknown. In this study, we showed that miR-101 was underexpressed in OSCC tissues and cell lines. miR-101 downregulation was inversely correlated with zinc finger E-box binding homeobox 1 (ZEB1) expression, lymph-node metastasis, and poor prognosis in OSCC patients. Enhanced expression of miR-101 significantly inhibited OSCC cell proliferation, apoptosis resistance, migration and invasion in vitro, and suppressed tumor growth and lung metastasis in vivo. Bioinformatics analyses showed that miR-101 directly targeted ZEB1, as confirmed by a dual-luciferase reporter assay. The inhibitory effects of miR-101 on OSCC growth and metastasis were attenuated and phenocopied by ZEB1 overexpression and knockdown, respectively. Overall, our findings indicated that miRNA-101 reduced OSCC growth and metastasis by targeting ZEB1 and provided new evidence of miR-101 as a potential therapeutic target for OSCC patients.
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University No. 145 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Baolei
authors.initials: B
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University No. 145 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Delin
authors.initials: D
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University No. 145 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University No. 145 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University No. 145 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Sen
authors.initials: S
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University No. 145 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Zihui
authors.initials: Z
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University No. 145 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Chun
authors.initials: C
authors.lastname: Shan
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University No. 145 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Shanghai Key Laboratory of Stomatology, Department of Oral & Maxillofacial-Head & Neck Oncology, Ninth People's Hospital, School of Stomatology, Shanghai Jiao Tong University School of Medicine No. 639 Zhizaoju Road, Shanghai 200011, PR China.
authors.forename: Chenping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University No. 145 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
keywords: metastasis; microRNA-101; oral squamous-cell carcinoma; proliferation; zinc finger E-box binding homeobox 1