title: Preparation of Well-Defined DNA Samples for Reproducible Nanospectroscopic Measurements.
abstract: Due to its well-defined topology and chemical structure, DNA could become a biological standard sample in the field of nanospectroscopy. Tip-enhanced Raman spectroscopy (TERS) provides new insights into individual DNA molecules immobilized on flat mica crystals. The high sensitivity of TERS is used to assess the chemical changes that appear in DNA upon different surface immobilization protocols.
authors.affiliation: Laboratory of Organic Chemistry, Department of Chemistry and Applied Biosciences, ETH Zurich, 8093, Zurich, Switzerland.|The Henryk Niewodniczanski Institute of Nuclear Physics, Polish Academy of Sciences, 31-342, Krakow, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Lipiec
authors.affiliation: Laboratory of Physics of Living Matter, Ecole Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.
authors.forename: Aleksandre
authors.initials: A
authors.lastname: Japaridze
authors.affiliation: Laboratory of Organic Chemistry, Department of Chemistry and Applied Biosciences, ETH Zurich, 8093, Zurich, Switzerland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Szczerbiński
authors.affiliation: Laboratory of Physics of Living Matter, Ecole Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Dietler
authors.affiliation: Laboratory of Organic Chemistry, Department of Chemistry and Applied Biosciences, ETH Zurich, 8093, Zurich, Switzerland. zenobi@org.chem.ethz.ch.
authors.forename: Renato
authors.initials: R
authors.lastname: Zenobi
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D025341:Principal Component Analysis; D011437:Propylamines / Q000737:chemistry; D015203:Reproducibility of Results; D012821:Silanes / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D000538:Aluminum Silicates; D011437:Propylamines; D012821:Silanes; D004247:DNA; C477625:amino-propyl-triethoxysilane; C011934:mica
keywords: DNA; DNA conformation; TERS, nanospectroscopy