title: Label-free live cell imaging by Confocal Raman Microscopy identifies CHO host and producer cell lines.
abstract: As a possible viable and non-invasive method to identify high producing cells, Confocal Raman Microscopy was shown to be able to differentiate CHO host cell lines and derivative production clones. Cluster analysis of spectra and their derivatives was able to differentiate between different producer cell lines and a host, and also distinguished between an intracellular region of high lipid and protein content that in structure resembles the Endoplasmic Reticulum. This ability to identify the ER may be a major contributor to the identification of high producers. PCA enabled the discrimination even of host cell lines and their subclones with inherently higher production capacity. The method is thus a promising option that may contribute to early, non-invasive identification of high potential candidates during cell line development and possibly could also be used for proof of identity of established production clones.
authors.affiliation: Institute of Physics and Materials Sciences, BOKU University of Natural Resources and Life Sciences Vienna, Vienna, Austria.
authors.forename: Batirtze
authors.initials: B
authors.lastname: Prats Mateu
authors.affiliation: Department of Biotechnology, BOKU University of Natural Resources and Life Sciences Vienna, Vienna, Austria.
authors.forename: Eva
authors.initials: E
authors.lastname: Harreither
authors.affiliation: Department of Biotechnology, BOKU University of Natural Resources and Life Sciences Vienna, Vienna, Austria.
authors.forename: Markus
authors.initials: M
authors.lastname: Schosserer
authors.affiliation: ACIB Austrian Center of Industrial Biotechnology, Graz, Austria.
authors.forename: Verena
authors.initials: V
authors.lastname: Puxbaum
authors.affiliation: Department of Biotechnology, BOKU University of Natural Resources and Life Sciences Vienna, Vienna, Austria.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Gludovacz
authors.affiliation: Department of Biotechnology, BOKU University of Natural Resources and Life Sciences Vienna, Vienna, Austria.|ACIB Austrian Center of Industrial Biotechnology, Graz, Austria.
authors.forename: Nicole
authors.initials: N
authors.lastname: Borth
authors.affiliation: Institute of Physics and Materials Sciences, BOKU University of Natural Resources and Life Sciences Vienna, Vienna, Austria.
authors.forename: Notburga
authors.initials: N
authors.lastname: Gierlinger
authors.affiliation: Department of Biotechnology, BOKU University of Natural Resources and Life Sciences Vienna, Vienna, Austria.|ACIB Austrian Center of Industrial Biotechnology, Graz, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Grillari
mesh_terms: D000068879:Adalimumab / Q000235:genetics / Q000378:metabolism; D006631:Amine Oxidase (Copper-Containing) / Q000235:genetics / Q000378:metabolism; D000818:Animals; D016466:CHO Cells / Q000166:cytology* / Q000648:ultrastructure*; D016000:Cluster Analysis; D003412:Cricetulus; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008670:Metals / Q000737:chemistry; D018613:Microscopy, Confocal / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods; D025341:Principal Component Analysis; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000379:methods
chemical_list: D008055:Lipids; D008670:Metals; D011506:Proteins; D011994:Recombinant Proteins; D006631:Amine Oxidase (Copper-Containing); D000068879:Adalimumab
keywords: Chinese hamster ovary cells; Label-free detection; Raman microscopy; Single cell analysis