title: Effects of PUFAs in a Mouse Model of HSV-1 Chorioretinitis.
abstract: OBJECTIVE
  To examine the effects of n-3 and n-6 polyunsaturated fatty acids (n-3 and n-6 PUFAs) in a murine model of herpetic chorioretinitis.
  
  
  METHODS
  BALB/c mice were fed on three high fat diets, which contained: Menhaden oil (rich in n-3 PUFAs); Safflower oil (rich in n-6 PUFAs); or Corn oil (rich in saturated fatty acids) as control group, 14 days previously and until 12 days following anterior chamber (AC) HSV-1 inoculation.
  
  
  RESULTS
  Mice fed on Menhaden oil present an early development of contralateral chorioretinitis by day 6 post-AC HSV-1 inoculation and also significant increase of RNA HSV-1 expression compared with Safflower and Corn oil groups. Furthermore, mice fed on Menhaden oil showed a significant decrease secretion of TNF-α, IFN-γ, IL-2 and IL-10 in splenic cells and both retinas.
  
  
  CONCLUSIONS
  Our results showed that mice fed on Menhaden oil (n-3 PUFAs) presented an early development of contralateral chorioretinitis by day 6 post-AC HSV-1 inoculation and also a significant increase in RNA HSV-1 expression compared with animals fed on Safflower and Corn oils. This increase of HSV-1 could be associated with the higher development of chorioretinitis.
authors.affiliation: a Centro de Imnunopatologias, Departamento de Patologia, Facultad de Medicina , Universidad de Buenos Aires , Argentina.|b Division Oftalmologia , Hospital de Clinicas, Universidad de Buenos Aires , Argentina.
authors.forename: Alejandro
authors.identifier: http://orcid.org/0000-0003-1577-7288
authors.initials: A
authors.lastname: Berra
authors.affiliation: a Centro de Imnunopatologias, Departamento de Patologia, Facultad de Medicina , Universidad de Buenos Aires , Argentina.
authors.forename: Julia
authors.initials: J
authors.lastname: Tau
authors.affiliation: a Centro de Imnunopatologias, Departamento de Patologia, Facultad de Medicina , Universidad de Buenos Aires , Argentina.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Zapata
authors.affiliation: a Centro de Imnunopatologias, Departamento de Patologia, Facultad de Medicina , Universidad de Buenos Aires , Argentina.|b Division Oftalmologia , Hospital de Clinicas, Universidad de Buenos Aires , Argentina.
authors.forename: Pablo
authors.initials: P
authors.lastname: Chiaradia
mesh_terms: D000818:Animals; D002825:Chorioretinitis / Q000821:virology*; D003314:Corn Oil / Q000008:administration & dosage; D004195:Disease Models, Animal; D015828:Eye Infections, Viral / Q000821:virology*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D043371:Fatty Acids, Omega-6 / Q000494:pharmacology*; D005395:Fish Oils / Q000008:administration & dosage; D006566:Herpesviridae Infections / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012450:Safflower Oil / Q000008:administration & dosage; D013937:Thymidine Kinase / Q000235:genetics; D014606:Uveitis, Anterior / Q000821:virology
chemical_list: D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D005395:Fish Oils; D012367:RNA, Viral; C033266:Menhaden oil; D012450:Safflower Oil; D003314:Corn Oil; D013937:Thymidine Kinase