title: Targeting the transforming growth factor-β signaling during pre-implantation development in embryos of cattle, sheep and goats.
abstract: Recently, application of chemical inhibitors against differentiation signaling pathways has improved establishment of mESCs. In this study, we applied inhibitors of TGF-β (SB431542) and BMP4 (Noggin) from cleavage to blastocyst stage in cattle, goat and sheep embryos. SB significantly decreases blastocyst rate and total cell number (TCN) in sheep blastocysts, whereas only TCN was significantly decreased in cattle blastocysts. In contrast to SB, Noggin significantly improved cattle blastocyst development but decreased TCN. However, Noggin treatment led to a significant increase in TCN in sheep blastocysts. Regarding pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment (REX1, CDX2, GATA4), SB led to a significant reduction in SOX2 expression in goat and cattle, while Noggin increased at least one or two of pluripotent markers in these species. Taken together, this data suggests that inhibition of TGF-β by Noggin may be more favorable for derivation of stem cells in farm animals.
authors.affiliation: a Department of Reproduction and Development , Royan Institute for Biotechnology, ACECR , Isfahan , Iran and.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hajian
authors.affiliation: a Department of Reproduction and Development , Royan Institute for Biotechnology, ACECR , Isfahan , Iran and.
authors.forename: Sayyed Morteza
authors.initials: SM
authors.lastname: Hosseini
authors.affiliation: a Department of Reproduction and Development , Royan Institute for Biotechnology, ACECR , Isfahan , Iran and.
authors.forename: Somayyeh
authors.initials: S
authors.lastname: Ostadhosseini
authors.affiliation: a Department of Reproduction and Development , Royan Institute for Biotechnology, ACECR , Isfahan , Iran and.|b Department of Embryology , Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR , Tehran , Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Nasr-Esfahani
mesh_terms: D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D001755:Blastocyst / Q000187:drug effects* / Q000378:metabolism; D019485:Bone Morphogenetic Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D000071616:CDX2 Transcription Factor / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D004149:Dioxoles / Q000494:pharmacology; D010064:Embryo Implantation; D005260:Female; D050991:GATA4 Transcription Factor / Q000235:genetics / Q000378:metabolism; D006041:Goats; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D000071317:Nanog Homeobox Protein / Q000235:genetics / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D012756:Sheep; D015398:Signal Transduction; D013045:Species Specificity; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: C459179:4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide; D001549:Benzamides; D019485:Bone Morphogenetic Proteins; D000071616:CDX2 Transcription Factor; D004149:Dioxoles; D050991:GATA4 Transcription Factor; D051741:Kruppel-Like Transcription Factors; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D055748:SOXB1 Transcription Factors; D016212:Transforming Growth Factor beta
keywords: BMP4; Blastocyst formation; TGFβ; embryonic stem cell; small molecule