title: Highly efficient production of D-lactic acid from chicory-derived inulin by Lactobacillus bulgaricus.
abstract: Inulin is a readily available feedstock for cost-effective production of biochemicals. To date, several studies have explored the production of bioethanol, high-fructose syrup and fructooligosaccharide, but there are no studies regarding the production of D-lactic acid using inulin as a carbon source. In the present study, chicory-derived inulin was used for D-lactic acid biosynthesis by Lactobacillus bulgaricus CGMCC 1.6970. Compared with separate hydrolysis and fermentation processes, simultaneous saccharification and fermentation (SSF) has demonstrated the best performance of D-lactic acid production. Because it prevents fructose inhibition and promotes the complete hydrolysis of inulin, the highest D-lactic acid concentration (123.6 ± 0.9 g/L) with a yield of 97.9 % was obtained from 120 g/L inulin by SSF. Moreover, SSF by L. bulgaricus CGMCC 1.6970 offered another distinct advantage with respect to the higher optical purity of D-lactic acid (>99.9 %) and reduced number of residual sugars. The excellent performance of D-lactic acid production from inulin by SSF represents a high-yield method for D-lactic acid production from non-food grains.
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People's Republic of China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Xu
authors.affiliation: College of Forestry, Nanjing Forestry University, Nanjing, 210037, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zang
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Forestry, Nanjing Forestry University, Nanjing, 210037, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yong
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People's Republic of China. hgouyj@njfu.edu.cn.|Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037, People's Republic of China. hgouyj@njfu.edu.cn.
authors.forename: Jia
authors.initials: J
authors.lastname: Ouyang
mesh_terms: D018651:Cichorium intybus / Q000737:chemistry*; D007444:Inulin / Q000737:chemistry* / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis*; D048668:Lactobacillus delbrueckii / Q000254:growth & development*
chemical_list: D019344:Lactic Acid; D007444:Inulin
keywords: D-Lactic acid; Fermentation; Inulin; Inulinase; Lactobacillus bulgaricus