title: Resistin mediates tomato and broccoli extract effects on glucose homeostasis in high fat diet-induced obesity in rats.
abstract: BACKGROUND
  Resistin is an adipocyte hormone that regulates glucose metabolism. Elevated levels of resistin may cause insulin resistance. This may link obesity, and increased fat mass to type II diabetes and insulin resistance. We hypothesized that treatment with tomato and broccoli extracts regulates glucose homeostasis via modulation of resistin levels in high fat diet-induced obesity rats (HFD).
  
  
  METHODS
  Forty-eight male albino rats were divided into 8 groups as follows: control, HFD, stop fat diet (SD), Tomato 200 mg/kg (T200), Tomato 400 mg/kg (T400), Broccoli 200 mg/kg (B200), Broccoli 400 mg/kg (B400), and Chromax (CX). Treatment continued for 1 month. Serum levels of resistin, leptin, adiponectin, glucose and insulin were measured using ELISA and spectrophotometry.
  
  
  RESULTS
  Serum levels of resistin were significantly reduced in the T 200, T 400, B 200, B 400 and CX groups to: 4.13 ± 0.22 ng/ml, 1.51 ± 0.04 ng/ml, 4.13 ± 0.22 ng/ml, 2.32 ± 0.15 ng/ml and 1.37 ± 0.03 ng/ml, respectively, compared to HFD group and SD group (P value < 0.0001). Non-significant differences were found between T 400, B 400 and CX groups. Serum levels of leptin were significantly reduced in the T 400 (22.7 ± 0.84 pg/ml) group compared to the B 400 (41 ± 2.45 Pg/ml) and CX groups (45.7 ± 2.91 Pg/ml), P value < 0.001. Serum levels of adiponectin were significantly increased in the T 400 group (131 ± 3.84 pg/ml) compared to the CX group (112 ± 4.77 pg/ml), P value < 0.01.
  
  
  CONCLUSIONS
  Our results demonstrate that tomato and broccoli extract treatment regulates glucose homeostasis via reduction of serum resistin and may be a useful non-pharmacological therapy for obesity.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, MSA University, Giza, 11787, Egypt.
authors.forename: Nora M
authors.initials: NM
authors.lastname: Aborehab
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, MSA University, Giza, 11787, Egypt.
authors.forename: Mahitab H
authors.initials: MH
authors.lastname: El Bishbishy
authors.affiliation: Department of Physiology, Faculty of Medicine, Helwan University, Helwan, 11787, Egypt. nwaly@icloud.com.
authors.forename: Nermien E
authors.initials: NE
authors.lastname: Waly
mesh_terms: D052242:Adiponectin / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D001937:Brassica; D059305:Diet, High-Fat; D020738:Leptin / Q000097:blood; D018551:Solanum lycopersicum; D008297:Male; D009765:Obesity / Q000503:physiopathology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D052243:Resistin / Q000097:blood / Q000378:metabolism*
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D020738:Leptin; D010936:Plant Extracts; D052243:Resistin
keywords: Broccoli; Glucose homeostasis; Obesity; Resistin; Tomato