title: Amycolatopsis xuchangensis sp. nov. and Amycolatopsis jiguanensis sp. nov., isolated from soil.
abstract: Two novel actinomycetes, designated strains CFH S0322Tand CFH S01580T, were isolated from soil samples collected from Xuchang city and Ji Guan cave in Henan province, China, respectively. Their taxonomic positions were investigated by a polyphasic approach. The two isolates were Gram-reaction positive, non-motile, aerobic and catalase-positive actinomycetes. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the two isolates are closely related to the members of the genus Amycolatopsis, sharing high similarities with Amycolatopsis magusensis KCTC 29056T(99.1 %, CFH S0322T) and Amycolatopsis nigrescens DSM 44992T(98.1 %, CFH S01580T), respectively. The major whole cell sugars of strains CFH S0322Tand CFH S01580Twere arabinose and galactose and the predominant menaquinone was MK-9(H4). The genomic DNA G+C contents of strains CFH S0322Tand CFH S01580Twere 70.6 and 68.6 mol%, respectively. Morphological, phylogenetic and chemotaxonomic results suggest that strains CFH S0322Tand CFH S01580Tare representatives of two new species, for which the names Amycolatopsis xuchangensis sp. nov. and Amycolatopsis jiguanensis sp. nov. are proposed. The type strains are CFH S0322T(=NBRC 110769T=KCTC 39516T) and CFH S01580T(=DSM 101526T=CCTCC AA 2015032T).
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang, 453007, People's Republic of China.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Huang
authors.affiliation: College of Life Sciences and Technology, Synthetic Biology Medicine Institute, Xinxiang Medical University, Xinxiang, 453003, People's Republic of China.|Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ming
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang, 453007, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang, 453007, People's Republic of China.
authors.forename: Zhuo-Li
authors.initials: ZL
authors.lastname: Zhao
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang, 453007, People's Republic of China.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Meng
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang, 453007, People's Republic of China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: College of Information and Management, Guangxi Medical University, Nanning, 530021, People's Republic of China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China. liwenjun3@mail.sysu.edu.cn.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Science, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China. liwenjun3@mail.sysu.edu.cn.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang, 453007, People's Republic of China. niegx@htu.cn.
authors.forename: Guo-Xing
authors.initials: GX
authors.lastname: Nie
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D058889:Molecular Typing; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Amycolatopsis jiguanensis sp. nov.; Amycolatopsis xuchangensis sp. nov.; Polyphasic taxonomy