title: RAD SNP markers as a tool for conservation of dolphinfish Coryphaena hippurus in the Mediterranean Sea: Identification of subtle genetic structure and assessment of populations sex-ratios.
abstract: Dolphinfish is an important fish species for both commercial and sport fishing, but so far limited information is available on genetic variability and pattern of differentiation of dolphinfish populations in the Mediterranean basin. Recently developed techniques allow genome-wide identification of genetic markers for better understanding of population structure in species with limited genome information. Using restriction-site associated DNA analysis we successfully genotyped 140 individuals of dolphinfish from eight locations in the Mediterranean Sea at 3324 SNP loci. We identified 311 sex-related loci that were used to assess sex-ratio in dolphinfish populations. In addition, we identified a weak signature of genetic differentiation of the population closer to Gibraltar Strait in comparison to other Mediterranean populations, which might be related to introgression of individuals from Atlantic. No further genetic differentiation could be detected in the other populations sampled, as expected considering the known highly mobility of the species. The results obtained improve our knowledge of the species and can help managing dolphinfish stock in the future.
authors.affiliation: Department of Compared Biomedicine and Food Science, University of Padova, viale dell'Università, 16, 35020 Legnaro, Italy. Electronic address: francesco.maroso@studenti.unipd.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Maroso
authors.affiliation: Department of Compared Biomedicine and Food Science, University of Padova, viale dell'Università, 16, 35020 Legnaro, Italy.
authors.forename: Rafaella
authors.initials: R
authors.lastname: Franch
authors.affiliation: Department of Compared Biomedicine and Food Science, University of Padova, viale dell'Università, 16, 35020 Legnaro, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Dalla Rovere
authors.affiliation: Department STEBICEF, University of Palermo, CoNISMa, via Archirafi, 18, 90123 Palermo, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Arculeo
authors.affiliation: Department of Compared Biomedicine and Food Science, University of Padova, viale dell'Università, 16, 35020 Legnaro, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Bargelloni
mesh_terms: D063147:Animal Distribution; D000818:Animals; D003247:Conservation of Natural Resources / Q000379:methods*; D005260:Female; D005819:Genetic Markers; D008297:Male; D008522:Mediterranean Sea; D010473:Perciformes / Q000235:genetics / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide; D015183:Restriction Mapping / Q000662:veterinary; D012732:Sex Determination Analysis / Q000662:veterinary; D012744:Sex Ratio
chemical_list: D005819:Genetic Markers
keywords: 2bRAD; Genetic differentiation; Outliers; Sex determination markers