title: Bone Marrow-Derived Progenitor Cells Are Functionally Impaired in Ischemic Heart Disease.
abstract: To determine whether the presence of ischemic heart disease (IHD) per se, or rather the co-presence of heart failure (HF), is the primum movens for less effective stem cell products in autologous stem cell therapy, we assessed numbers and function of bone marrow (BM)-derived progenitor cells in patients with coronary artery disease (n = 17), HF due to ischemic cardiomyopathy (n = 8), non-ischemic HF (n = 7), and control subjects (n = 11). Myeloid and erythroid differentiation capacity of BM-derived mononuclear cells was impaired in patients with underlying IHD but not with non-ischemic HF. Migration capacity decreased with increasing IHD severity. Hence, IHD, with or without associated cardiomyopathy, is an important determinant of progenitor cell function. No depletion of hematopoietic and endothelial progenitor cells (EPC) within the BM was observed, while circulating EPC numbers were increased in the presence of IHD, suggesting active recruitment. The observed myelosuppression was not driven by inflammation and thus other mechanisms are at play.
authors.affiliation: Laboratory of Cellular and Molecular Cardiology, Department of Cardiology, Antwerp University Hospital, Antwerp, Belgium. Evelien.Nollet@uantwerpen.be.|Cardiovascular Diseases, Department of Translational Pathophysiological Research, University of Antwerp, Antwerp, Belgium. Evelien.Nollet@uantwerpen.be.
authors.forename: Evelien
authors.initials: E
authors.lastname: Nollet
authors.affiliation: Laboratory of Cellular and Molecular Cardiology, Department of Cardiology, Antwerp University Hospital, Antwerp, Belgium.|Cardiovascular Diseases, Department of Translational Pathophysiological Research, University of Antwerp, Antwerp, Belgium.
authors.forename: Vicky Y
authors.initials: VY
authors.lastname: Hoymans
authors.affiliation: Department of Cardiac Surgery, Antwerp University Hospital, Antwerp, Belgium.
authors.forename: Inez R
authors.initials: IR
authors.lastname: Rodrigus
authors.affiliation: Department of Cardiac Surgery, Antwerp University Hospital, Antwerp, Belgium.
authors.forename: Dina
authors.initials: D
authors.lastname: De Bock
authors.affiliation: Department of Oral and Maxillofacial Surgery, General Hospital Sint-Maarten, Duffel, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Dom
authors.affiliation: Department of Oral and Maxillofacial Surgery, General Hospital Monica, Antwerp, Belgium.
authors.forename: Bruno
authors.initials: B
authors.lastname: Vanassche
authors.affiliation: Department of Clinical Chemistry, Antwerp University Hospital, Antwerp, Belgium.|Biochemistry, Department of Translational Pathophysiological Research, University of Antwerp, Antwerp, Belgium.
authors.forename: Viviane O M
authors.initials: VO
authors.lastname: Van Hoof
authors.affiliation: Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Cools
authors.affiliation: Laboratory of Cellular and Molecular Cardiology, Department of Cardiology, Antwerp University Hospital, Antwerp, Belgium.|Cardiovascular Diseases, Department of Translational Pathophysiological Research, University of Antwerp, Antwerp, Belgium.
authors.forename: Katrijn
authors.initials: K
authors.lastname: Van Ackeren
authors.affiliation: Department of Scientific Coordination and Biostatistics, Antwerp University Hospital, Antwerp, Belgium.
authors.forename: Kristien
authors.initials: K
authors.lastname: Wouters
authors.affiliation: Laboratory of Hematology, Antwerp University Hospital, Antwerp, Belgium.
authors.forename: Katrien
authors.initials: K
authors.lastname: Vermeulen
authors.affiliation: Laboratory of Cellular and Molecular Cardiology, Department of Cardiology, Antwerp University Hospital, Antwerp, Belgium.|Cardiovascular Diseases, Department of Translational Pathophysiological Research, University of Antwerp, Antwerp, Belgium.|Department of Cardiology, Antwerp University Hospital, Antwerp, Belgium.
authors.forename: Christiaan J
authors.initials: CJ
authors.lastname: Vrints
authors.affiliation: Laboratory of Cellular and Molecular Cardiology, Department of Cardiology, Antwerp University Hospital, Antwerp, Belgium.|Cardiovascular Diseases, Department of Translational Pathophysiological Research, University of Antwerp, Antwerp, Belgium.|Department of Cardiology, Antwerp University Hospital, Antwerp, Belgium.
authors.forename: Emeline M
authors.initials: EM
authors.lastname: Van Craenenbroeck
mesh_terms: D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D042501:Angiogenic Proteins / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology*; D009202:Cardiomyopathies / Q000378:metabolism / Q000473:pathology*; D016022:Case-Control Studies; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D003324:Coronary Artery Disease / Q000378:metabolism / Q000473:pathology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D066026:Endothelial Progenitor Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000378:metabolism / Q000473:pathology*; D010641:Phenotype; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism
chemical_list: D042501:Angiogenic Proteins; D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators; D018121:Receptors, Cytokine; D000469:Alkaline Phosphatase
keywords: Bone marrow; Heart failure; Inflammation; Ischemic heart disease; Progenitor cells