title: Yeast surface display of dehydrogenases in microbial fuel-cells.
abstract: Two dehydrogenases, cellobiose dehydrogenase from Corynascus thermophilus and pyranose dehydrogenase from Agaricus meleagris, were displayed for the first time on the surface of Saccharomyces cerevisiae using the yeast surface display system. Surface displayed dehydrogenases were used in a microbial fuel cell and generated high power outputs. Surface displayed cellobiose dehydrogenase has demonstrated a midpoint potential of -28mV (vs. Ag/AgCl) at pH=6.5 and was used in a mediator-less anode compartment of a microbial fuel cell producing a power output of 3.3μWcm(-2) using lactose as fuel. Surface-displayed pyranose dehydrogenase was used in a microbial fuel cell and generated high power outputs using different substrates, the highest power output that was achieved was 3.9μWcm(-2) using d-xylose. These results demonstrate that surface displayed cellobiose dehydrogenase and pyranose dehydrogenase may successfully be used in microbial bioelectrochemical systems.
authors.affiliation: Department of Life Sciences and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, Israel.
authors.forename: Idan
authors.initials: I
authors.lastname: Gal
authors.affiliation: Department of Life Sciences and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, Israel.
authors.forename: Orr
authors.initials: O
authors.lastname: Schlesinger
authors.affiliation: Department of Life Sciences and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, Israel.
authors.forename: Liron
authors.initials: L
authors.lastname: Amir
authors.affiliation: Department of Life Sciences and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, Israel. Electronic address: alfontal@bgu.ac.il.
authors.forename: Lital
authors.initials: L
authors.lastname: Alfonta
mesh_terms: D000327:Adsorption; D000364:Agaricus / Q000201:enzymology; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D002237:Carbohydrate Dehydrogenases / Q000235:genetics* / Q000378:metabolism*; D004563:Electrochemistry; D004566:Electrodes; D008751:Methylene Blue / Q000378:metabolism; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D020037:Sordariales / Q000201:enzymology; D062065:Wastewater / Q000382:microbiology
chemical_list: D002237:Carbohydrate Dehydrogenases; D008751:Methylene Blue; D062065:Wastewater; C019859:cellobiose-quinone oxidoreductase
keywords: Cellobiose dehydrogenase; Enzymatic fuel cells; Microbial fuel cells; Pyranose dehydrogenase; Yeast surface display