title: Photodynamic therapy effect on cell growth inhibition induced by Radachlorin and toluidine blue O on Staphylococcus aureus and Escherichia coli: An in vitro study.
abstract: BACKGROUND
  Photodynamic therapy is an innovative treatment modality, which is appropriate for tumor detection and for the treatment of cancer as well as nontumoral diseases, such as psoriasis (2), bacterial and viral eradication.
  
  
  METHODS
  Effect of two photosensitizer (toluidine blue O (TBO) and Radachlorin was investigated on Staphylococcus Aureus ATCC 25923 (American Type Culture Collection) and Escherichia coli (ATCC 25922).
  
  
  RESULTS
  PDI by TBO caused S. aureus 5.83 log10 killing (P.Value<0.0001) and reduce 0.08 log 10 in E. coli (P.Value=0.321). PDI by Radachlorin(®) reduce 0.17 log 10 in E. coli (P.Value<0.0001) and S. aureus showed 6.1 log 10 colony count reduction.
  
  
  CONCLUSIONS
  Within the limitation of this in vitro study, we can conclude that both PS have the same effect on S. aureus and E. coli with good inhibition effect on S. aureus and partial inhibition effect E. coli.
authors.affiliation: Department of Periodontology, Dental Faculty - Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran; International Network for Photo Medicine and Photo Dynamic Therapy (INPMPDT), Universal Scientific Education and Research Network (USERN), Tehran, Iran. Electronic address: rezafekrazad@gmail.com.
authors.forename: Reza
authors.initials: R
authors.lastname: Fekrazad
authors.affiliation: Periodontic Department, Faculty of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran. Electronic address: dr_hadizare@yahoo.com.
authors.forename: Hadi
authors.initials: H
authors.lastname: Zare
authors.affiliation: Operative dentistry Department, Faculty of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran. Electronic address: smohammadis66@gmail.com.
authors.forename: Sara Mohammadi Sepah
authors.initials: SM
authors.lastname: Vand
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004338:Drug Combinations; D004359:Drug Therapy, Combination / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008027:Light; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage; D011166:Porphyrins / Q000008:administration & dosage*; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology; D014048:Tolonium Chloride / Q000008:administration & dosage*
chemical_list: D004338:Drug Combinations; D017319:Photosensitizing Agents; D011166:Porphyrins; C000593241:bremachlorin; D014048:Tolonium Chloride
keywords: PDI; Photodynamic therapy; Radachlorin; Toluidine blue O (TBO)