title: De Novo Sequencing and Characterization of the Transcriptome of Dwarf Polish Wheat (Triticum polonicum L.).
abstract: Construction as well as characterization of a polish wheat transcriptome is a crucial step to study useful traits of polish wheat. In this study, a transcriptome, including 76,014 unigenes, was assembled from dwarf polish wheat (DPW) roots, stems, and leaves using the software of Trinity. Among these unigenes, 61,748 (81.23%) unigenes were functionally annotated in public databases and classified into differentially functional types. Aligning this transcriptome against draft wheat genome released by the International Wheat Genome Sequencing Consortium (IWGSC), 57,331 (75.42%) unigenes, including 26,122 AB-specific and 2,622 D-specific unigenes, were mapped on A, B, and/or D genomes. Compared with the transcriptome of T. turgidum, 56,343 unigenes were matched with 103,327 unigenes of T. turgidum. Compared with the genomes of rice and barley, 14,404 and 7,007 unigenes were matched with 14,608 genes of barley and 7,708 genes of rice, respectively. On the other hand, 2,148, 1,611, and 2,707 unigenes were expressed specifically in roots, stems, and leaves, respectively. Finally, 5,531 SSR sequences were observed from 4,531 unigenes, and 518 primer pairs were designed.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Yi
authors.identifier: 0000-0003-0018-2716
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Peng
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Ruijiao
authors.initials: R
authors.lastname: Wang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Resources, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Fan
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Houyang
authors.initials: H
authors.lastname: Kang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Sha
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Haiqin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
authors.forename: Yonghong
authors.identifier: 0000-0003-4939-1383
authors.initials: Y
authors.lastname: Zhou