title: Analysis of metalaxyl racemate using high performance liquid chromatography coupled with four kinds of detectors.
abstract: Chiral stationary phase-high performance liquid chromatography coupled with various detectors has been one of most commonly used methods for analysis and separation of chiral compounds over the past decades. Various detectors exhibit different characteristics in qualitative and quantitative studies under different chromatographic conditions. Herein, a comparative evaluation of HPLC coupled with ultraviolet, optical rotation, refractive index, and evaporative light scattering detectors has been conducted for qualitative and quantitative analyses of metalaxyl racemate. Effects of separation conditions on the peak area ratio between two enantiomers, including sample concentration, column temperature, mobile phase composition, as well as flow rate, have been investigated in detail. In addition, the limits of detection, the limits of quantitation, quantitative range and precision for these two enantiomers by using four detectors have been also studied. As indicated, the chromatographic separation conditions have been slight effects on ultraviolet and refractive index detections and the peak area ratio between two enantiomers remains almost unchanged, but the evaporative light scattering detection has been significantly affected by the above-mentioned chromatographic conditions and the corresponding peak area ratios varied greatly. Moreover, the limits of detection, the limits of quantitation, and the quantitative ranges of two enantiomers with UV detection were remarkably lower by 1-2 magnitudes than the others.
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China. Electronic address: fanj@scnu.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Fan
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Gao
authors.affiliation: Guangzhou Research & Creativity Biotechnology Co., Ltd, Guangzhou 510663, China.
authors.forename: Tai
authors.initials: T
authors.lastname: Wang
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China; Guangzhou Research & Creativity Biotechnology Co., Ltd, Guangzhou 510663, China. Electronic address: wgzhang@scnu.edu.cn.
authors.forename: Weiguang
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D057230:Limit of Detection; D012031:Refractometry; D013237:Stereoisomerism; D013696:Temperature
chemical_list: C028175:metalaxyl; D000409:Alanine
keywords: Chromatographic detectors; Enantiomeric ratio; HPLC chiral analysis; Metalaxyl; Quantitative study