title: Investigation of the oxidative stress condition for occupational exposure to methyl methacrylate.
abstract: The purpose of this study is the evaluation of possible toxicological effects on dental personnel, who are exposed to the production materials of prostheses during their entire working life. In this study, the level of methyl methacrylate (MMA) in ambient air was measured as an indicator of external exposure on the personnel. In order to evaluate the possible toxic effects of oxidative stress, which is thought to play an important role in the formation process of many diseases such as cancer, blood samples of volunteers were examined via the ultraviolet-spectroscopic determination of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase antioxidant enzyme activities, comparing occupationally MMA-exposed ( n = 69) and control ( n = 67) groups. In these groups, malondialdehyde (MDA) and glutathione levels were also determined by the same method. The results showed that SOD activity and MDA and glutathione levels were significantly higher in the exposed group when compared with the control group ( p < 0.001). A significant decrease was determined in the glutathione peroxidase activities of the exposed group ( p < 0.05). No significant difference in CAT activities between the groups has been found. When the results obtained in the study are considered, it is suggested that an adaptive response might be developed by dental technicians against low-level MMA exposure.
authors.affiliation: 1 Department of Pharmaceutical Toxicology, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Buğra
authors.initials: B
authors.lastname: Soykut
authors.affiliation: 1 Department of Pharmaceutical Toxicology, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Onur
authors.initials: O
authors.lastname: Erdem
authors.affiliation: 1 Department of Pharmaceutical Toxicology, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Cemal
authors.initials: C
authors.lastname: Akay
authors.affiliation: 2 Department of Prosthodontics, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Bülent
authors.initials: B
authors.lastname: Pişkin
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D003815:Dentists; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D020366:Methylmethacrylate / Q000633:toxicity*; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D017382:Reactive Oxygen Species; D020366:Methylmethacrylate; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Antioxidant; adaptive response; genotoxicity; glutathione; oxidative stress