title: Peptide Aβ(16-25) Forms Nanofilms in the Process of Its Aggregation.
abstract: A method for the synthesis and high purification of fragments of Aβ(1-42) peptide has been elaborated. We have synthesized the amyloidogenic fragment Aβ(16-25) predicted by us and studied the process of its aggregation by electron microscopy and X-ray analysis. Electron microscopy images show that the peptide forms a film, which is not characteristic of amyloid fibrils. At the same time, according to the X-ray diffraction data, its preparations display the presence of two main reflections (4.6-4.8 and 8-12 Å) characteristic of cross-β structure of amyloid fibrils. Thus, the fragment Aβ(16-25) that we predicted is a promising object not only for studying the process of polymerization of the peptides/proteins, but also for using it as a nanomaterial to study a number of biological processes.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. ogalzit@vega.protres.ru.
authors.forename: O M
authors.initials: OM
authors.lastname: Selivanova
authors.forename: E Yu
authors.initials: EY
authors.lastname: Gorbunova
authors.forename: L G
authors.initials: LG
authors.lastname: Mustaeva
authors.forename: E I
authors.initials: EI
authors.lastname: Grigorashvili
authors.forename: M Yu
authors.initials: MY
authors.lastname: Suvorina
authors.forename: A K
authors.initials: AK
authors.lastname: Surin
authors.forename: O V
authors.initials: OV
authors.lastname: Galzitskaya
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D018360:Crystallography, X-Ray; D008854:Microscopy, Electron; D049329:Nanostructures / Q000737:chemistry*; D017433:Protein Structure, Secondary; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides