title: Malaria-Cutaneous Leishmaniasis Co-infection: Influence on Disease Outcomes and Immune Response.
abstract: Malaria and Cutaneous Leishmaniasis (CL) are co-endemic throughout large regions in tropical countries and co-infection may impact the evolution of host-parasite interactions. In the present study, we evaluate Malaria/Leishmaniasis disease outcome, Th1/Th2 cytokine levels and the CD4 and CD8 T-cell profiles in a co-infection murine model (BALB/c) of Plasmodium yoelii 17XNL (Py) and Leishmania amazonensis (La) or L. braziliensis (Lb). Malaria parasitaemia was assessed through blood strains stained with Giemsa. Leishmania lesions were monitored with a digital caliper and parasite loads determined by limiting-dilution assay. Serum levels of IFN-γ, TNF, IL-2, IL-4, IL-6, IL-10, and IL-17 were determined using multiplexed bead assay and expression of CD3, CD4, and CD8 T-cells markers were determined by Flow Cytometry in the thymus, spleens and lymph nodes. Parasitaemia in Lb+Py co-infected group was lower than in Py single-infected group, suggesting a protective effect of Lb co-infection in Malaria progression. In contrast, La+Py co-infection increased parasitaemia, patent infection and induced mortality in non-lethal Malaria infection. Regarding Leishmaniasis, Lb+Py co-infected group presented smaller lesions and less ulceration than Lb single-infected animals. In contrast, La+Py co-infected group presented only a transitory delay on the development of lesions when compared to La single-infected mice. Decreased levels of IFN-γ, TNF, IL-6, and IL-10 were observed in the serum of co-infected groups, demonstrating a modulation of Malaria immune response by Leishmania co-infections. We observed an intense thymic atrophy in Py single-infected and co-infected groups, which recovered earlier in co-infected animals. The CD4 and CD8 T cell profiles in thymus, spleens and lymph nodes did not differ between Py single and co-infected groups, except for a decrease in CD4(+)CD8(+) T cells which also increased faster in co-infected mice. Our results suggest that Py and Leishmania co-infection may change disease outcome. Interestingly Malaria outcome can be altered according to the Leishmania specie involved. Alternatively Malaria infection reduced the severity or delayed the onset of leishmanial lesions. These alterations in Malaria and CL development seem to be closely related with changes in the immune response as demonstrated by alteration in serum cytokine levels and thymus/spleens T cell phenotypes dynamics during infection.
authors.affiliation: Laboratory of Simulids, Onchocerciasis and Sympatric Diseases: Mansonelliasis and Malaria, Oswaldo Cruz Institute, Oswaldo Cruz Foundation Rio de Janeiro, Brazil.
authors.forename: Raquel A
authors.initials: RA
authors.lastname: Pinna
authors.affiliation: Laboratory of Thymus Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation Rio de Janeiro, Brazil.
authors.forename: Danielle
authors.initials: D
authors.lastname: Silva-Dos-Santos
authors.affiliation: Laboratory of Simulids, Onchocerciasis and Sympatric Diseases: Mansonelliasis and Malaria, Oswaldo Cruz Institute, Oswaldo Cruz Foundation Rio de Janeiro, Brazil.
authors.forename: Daiana S
authors.initials: DS
authors.lastname: Perce-da-Silva
authors.affiliation: Laboratory of Immunoparasitology Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation Rio de Janeiro, Brazil.
authors.forename: Joseli
authors.initials: J
authors.lastname: Oliveira-Ferreira
authors.affiliation: Laboratory of Thymus Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation Rio de Janeiro, Brazil.
authors.forename: Dea M S
authors.initials: DM
authors.lastname: Villa-Verde
authors.affiliation: Laboratory of Immunoparasitology Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation Rio de Janeiro, Brazil.
authors.forename: Paula M
authors.initials: PM
authors.lastname: De Luca
authors.affiliation: Laboratory of Simulids, Onchocerciasis and Sympatric Diseases: Mansonelliasis and Malaria, Oswaldo Cruz Institute, Oswaldo Cruz Foundation Rio de Janeiro, Brazil.
authors.forename: Dalma M
authors.initials: DM
authors.lastname: Banic
keywords: Leishmania amazonensis; Leishmania braziliensis; Leishmaniasis; Malaria; Plasmodium yoelii; co-infection; cytokine; immunology