title: Determinants of Genomic RNA Encapsidation in the Saccharomyces cerevisiae Long Terminal Repeat Retrotransposons Ty1 and Ty3.
abstract: Long-terminal repeat (LTR) retrotransposons are transposable genetic elements that replicate intracellularly, and can be considered progenitors of retroviruses. Ty1 and Ty3 are the most extensively characterized LTR retrotransposons whose RNA genomes provide the template for both protein translation and genomic RNA that is packaged into virus-like particles (VLPs) and reverse transcribed. Genomic RNAs are not divided into separate pools of translated and packaged RNAs, therefore their trafficking and packaging into VLPs requires an equilibrium between competing events. In this review, we focus on Ty1 and Ty3 genomic RNA trafficking and packaging as essential steps of retrotransposon propagation. We summarize the existing knowledge on genomic RNA sequences and structures essential to these processes, the role of Gag proteins in repression of genomic RNA translation, delivery to VLP assembly sites, and encapsidation.
authors.affiliation: Laboratory of Structural Chemistry and Biology of Nucleic Acids, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan 61-704, Poland. kasiapw@ibch.poznan.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pachulska-Wieczorek
authors.affiliation: RT Biochemistry Section, Basic Research Laboratory, National Cancer Institute, Frederick, MD 21702, USA. legrices@mail.nih.gov.
authors.forename: Stuart F J
authors.initials: SF
authors.lastname: Le Grice
authors.affiliation: Laboratory of Structural Chemistry and Biology of Nucleic Acids, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan 61-704, Poland. purzycka@ibch.poznan.pl.
authors.forename: Katarzyna J
authors.initials: KJ
authors.lastname: Purzycka
mesh_terms: D001692:Biological Transport; D011485:Protein Binding; D012331:RNA, Fungal / Q000378:metabolism*; D018626:Retroelements; D048348:Reverse Transcription; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D020079:Terminal Repeat Sequences
chemical_list: D012331:RNA, Fungal; D018626:Retroelements
keywords: Gag protein; RNA packaging; RNA trafficking; Ty1 retrotransposon; Ty3 retrotransposon