title: Effect of IL-6 and IL-8 on the expression of the complement activation inhibitors MAC-inhibitory protein and decay-accelerating factor in ovarian cancer A2780 cells.
abstract: The aim of the present study was to evaluate the role of interleukin (IL)-6 and IL-8 on the expression of the membrane-bound complement inhibitors membrane attack complex-inhibitory protein (CD59) and decay-accelerating factor (CD55), in the human ovarian carcinoma A2780 cell line, which is a non-producing IL-6 cell line that does exhibit IL-6 responsiveness, due to the presence of IL-6 receptors. Extracellular levels of complement system inhibitors were evaluated by western blotting and reverse transcription-quantitative polymerase chain reaction. Cellular localization of CD55 and CD59 in the ovarian cancer cells was assessed by immunofluorescence. The detection of a soluble form of CD55 and CD59 released by the A2780 cells following stimulation with IL-6 and IL-8 was detected by enzyme-linked immunosorbent assay. The present data revealed that A2780 cells express CD55 and CD59 at the mRNA and protein level, but do not secrete these proteins to the culture medium. Results of western blotting demonstrated that the protein level of CD59 was regulated by IL-6 and IL-8 in a dose-dependent manner. Immunofluorescence analysis revealed that the ovarian cancer A2780 cell line expresses the membrane bound form of CD55 protein. The present results indicate that CD55 and CD59 may affect the efficiency of complement-mediated immunotherapies.
authors.affiliation: Department of Medical Biology and Translational Research, Faculty of Medicine, University of Information Technology and Management, 35-225 Rzeszow, P.R. China.
authors.forename: Lucyna
authors.initials: L
authors.lastname: Kapka-Skrzypczak
authors.affiliation: Department of Molecular Biology and Translational Research, Institute of Rural Health, 20-090 Lublin, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Popek
authors.affiliation: Department of Molecular Biology and Translational Research, Institute of Rural Health, 20-090 Lublin, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Sawicki
authors.affiliation: Department of Pathology, Medical University of Warsaw, 02-091 Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Wolińska
authors.affiliation: Department of Molecular Biology and Translational Research, Institute of Rural Health, 20-090 Lublin, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Czajka
authors.affiliation: Second Department of Gynecology, Medical University of Lublin, 20-954 Lublin, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Skrzypczak
keywords: CD55; CD59; IL-6; complement system; ovarian cancer