title: Physiological and proteomic analysis of selenium-mediated tolerance to Cd stress in cucumber (Cucumis sativus L.).
abstract: Selenium can mitigate cadmium toxicity in plants. However, the mechanism of this alleviation has not been fully understood. In the present study, the role of Se in inducing tolerance to Cd stress in cucumber was elucidated. Results showed that Se significantly alleviated Cd-induced growth inhibition, reduced Cd concentration, increased SPAD value and improved photosynthetic performance. Through proteomic analysis by two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry, 26 protein spots were identified, which were significantly influenced by Cd stress and/or Se application. Among these proteins, the abundance of 21 spots (10 in leaves and 11 in roots) were repressed in Cd-treated and up-accumulated or no-changed in Cd+Se-treated cucumber. These altered proteins were involved in the response to stress, metabolism, photosynthesis and storage, they were including glutathione S-transferase F8, heat shock protein STI-like, peroxidase, ascorbate oxidase, fructose-bisphosphate aldolase 2, NiR, Rieske type ion sulfur subunit and PsbP domain-containing protein 6. Furthermore, we identified five proteins with an increase in relative abundance after Cd treatment, they were involved in the functional groups active in response to stress and transport. The present study provided novel insights into Se-mediated tolerance of cucumber seedlings against Cd toxicity at the proteome level.
authors.affiliation: College of Chemical and Biological Engineering, Taiyuan University of Science and Technology, Taiyuan 030024, PR China. Electronic address: sunhongyan-8@163.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Sun
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, PR China.
authors.forename: Huaxin
authors.initials: H
authors.lastname: Dai
authors.affiliation: Institute of Shanxi Soil and Water Conservation, Taiyuan 030045, PR China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Chemical and Biological Engineering, Taiyuan University of Science and Technology, Taiyuan 030024, PR China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000975:Antioxidants / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D018553:Cucumis sativus / Q000187:drug effects* / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D005982:Glutathione Transferase / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D018384:Oxidative Stress / Q000187:drug effects*; D010788:Photosynthesis / Q000187:drug effects / Q000502:physiology; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis*; D018517:Plant Roots / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D012643:Selenium / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D020543:Proteome; D002104:Cadmium; D005982:Glutathione Transferase; D012643:Selenium
keywords: Cadmium; Cucumber (Cucumis sativus L.); Photosynthesis; Proteomic analysis; Selenium