title: A three phase hollow fiber liquid-phase microextraction for quantification of lamotrigine in plasma of epileptic patients by capillary electrophoresis.
abstract: A three phase hollow fiber liquid-phase microextraction technique combined with capillary electrophoresis was developed to quantify lamotrigine (LTG) in plasma samples. The analyte was extracted from 4.0 mL of a basic donor phase (composed of 0.5 mL of plasma and 3.5 mL of sodium phosphate solution pH 9.0) through a supported liquid membrane composed of 1-octanol immobilized in the pores of the hollow fiber, and to an acidic acceptor phase (hydrochloric acid solution pH 4.0) placed in the lumen of the fiber. The extraction was carried out for 30 min at 500 rpm. The eletrophoretic analysis was carried out in 130 mmol/L MES buffer, pH 5.0 with a constant voltage of +15 kV and 20°C. Sample injections were performed for 10 s, at a pressure of 0.5 psi. The detection was performed at 214 nm for both LTG and the internal standard lidocaine. Under the optimized conditions, the method showed a limit of quantification of 1.0 μg/mL and was linear over the plasmatic concentration range of 1.0-20.0 μg/mL. Finally, the validated method was applied for the quantification of LTG in plasma samples of epileptic patients.
authors.affiliation: Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Luiza Saldanha Ribeiro
authors.initials: LS
authors.lastname: Barros
authors.affiliation: Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Daniel Blascke
authors.initials: DB
authors.lastname: Carrão
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Regina Helena Costa
authors.initials: RH
authors.lastname: Queiroz
authors.affiliation: Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Anderson Rodrigo Moraes
authors.initials: AR
authors.lastname: de Oliveira
authors.affiliation: Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil. crisgai@fcfrp.usp.br.
authors.forename: Cristiane Masetto
authors.initials: CM
authors.lastname: de Gaitani
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D004827:Epilepsy / Q000188:drug therapy*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000077213:Lamotrigine; D057230:Limit of Detection; D016014:Linear Models; D059627:Liquid Phase Microextraction / Q000379:methods*; D015203:Reproducibility of Results; D014227:Triazines / Q000097:blood* / Q000627:therapeutic use
chemical_list: D014227:Triazines; D000077213:Lamotrigine
keywords: Capillary electrophoresis; Hollow fiber liquid-phase microextraction; Human plasma; Lamotrigine