title: Ratiometric Fluorescent pH Probes Based on Glycopolymers.
abstract: Effectively detecting pH changes plays a critical role in exploring cellular functions and determining physiological and pathological processes. A novel ratiometric pH probe based on a glycopolymer, armored with properties of serum-stability, tumor-targeting, and pH monitoring, is designed. Random copolymers of 2-(methacrylamido) glucopyranose and fluorescein O-methacrylate are first synthesized by reversible addition fragmentation chain transfer polymerization. Acryloxyethyl thiocarbamoyl rhodamine B is then attached to the polymer chain to prepare ratiometric fluorescent pH probes via a thiol-ene reaction. The synthesized polymeric probes are characterized by NMR, gel permeation chromatography, UV-vis spectroscopy, and transmission electron microscopy, and the fluorescence responses are examined in phosphate buffer at different pHs. The cytotoxicity and confocal imaging experiments of the probes are detected using HeLa cells, demonstrating a low toxicity and superior biocompatibility for detecting pH changes in bioapplications.
authors.affiliation: Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou, 215006, China.
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Li
authors.affiliation: Cyrus Tang Hematology Center, Soochow University, Suzhou, 215123, China.|The Collaborative Innovation Center of Hematology, Soochow University, Suzhou, 215006, China.
authors.forename: Pengshan
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou, 215006, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou, 215006, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Peng
authors.affiliation: Cyrus Tang Hematology Center, Soochow University, Suzhou, 215123, China. zhaoy@suda.edu.cn.|The Collaborative Innovation Center of Hematology, Soochow University, Suzhou, 215006, China. zhaoy@suda.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou, 215006, China. gchen@suda.edu.cn.
authors.forename: Gaojian
authors.initials: G
authors.lastname: Chen
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006001:Glycoconjugates / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010316:Particle Size; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005456:Fluorescent Dyes; D006001:Glycoconjugates; D011108:Polymers
keywords: cell imaging; glycopolymers; ratiometric pH probes; reversible addition fragmentation chain transfer (RAFT); thiol-ene chemistry