title: Isolation and identification of resveratrol-producing endophytes from wine grape Cabernet Sauvignon.
abstract: OBJECTIVE
  Obtain endophyte strains with effective resveratrol production from superior grapevine variety Cabernet Sauvignon in Xinjiang and determine related taxonomic position of the strain.
  
  
  RESULTS
  Seventy-three strains of endophytes, including 23 strains of bacteria, 14 ones of actinomycetes, 24 fungus and 12 yeasts, were isolated, respectively. The distribution law of endophytes was spring (30.14 %) = summer (30.14 %) < autumn (39.73 %) in different seasons, while the fruit (12.33 %) < leaf (20.55 %) < stem (32.88 %) < root (34.25 %) in different tissues and organs. From the 36 strains of endophytic fungi isolated, seven strains producing polyphenols were screened by ferric chloride-potassium ferricyanide color reaction. C2J6, stable genetic properties producing highly 1.48 mg L(-1) of resveratrol, was identified as Aspergillus niger by 26S rDNA-ITS sequence analysis after thin-layer chromatography sieve analysis, ultra violet wavelength scanning and high performance liquid chromatography, respectively.
  
  
  CONCLUSIONS
  There were the certain number and kinds of endophytes in the various tissues of Cabernet Sauvignon, which, to a certain extent, reflected the biological diversity of plant endophytes. The fact that the fungus C2J6 producing resveratrol in grape was acquired attested the special ability of the endophytes to produce the same or similar bioactive substances as the host plants.
authors.affiliation: College of Food, Shihezi University, Shihezi, 832000 People's Republic of China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Food, Shihezi University, Shihezi, 832000 People's Republic of China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Nan
authors.affiliation: College of Food, Shihezi University, Shihezi, 832000 People's Republic of China.
authors.forename: Junchao
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Food, Shihezi University, Shihezi, 832000 People's Republic of China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Yan
authors.affiliation: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097 People's Republic of China.
authors.forename: Dianpeng
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Institute of Crops, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, 832000 People's Republic of China.
authors.forename: Xinnian
authors.initials: X
authors.lastname: Han
keywords: Cabernet Sauvignon; Endophytes; Identification; Resveratrol; Screening