title: Genome sequence analysis of two South African isolates of Moroccan watermelon mosaic virus infecting cucurbits.
abstract: Moroccan watermelon mosaic virus (MWMV) has been prevalent in cucurbits in the Republic of South Africa (RSA) since it was first reported in 1987. However, full genome studies of the South African isolates have never been conducted previously. The full genome of two MWMV isolates infecting cucurbits (Cucurbita pepo L.) in the province of KwaZulu-Natal, RSA, was compared with the genome of the Tunisian isolate in this communication. The genome sequences of the RSA MWMV isolates were elucidated using next-generation sequencing and Sanger sequencing. The analyses performed included nucleotide and amino acid sequence comparison, determination of the genetic distances, detection of potential recombination, and phylogeny. The genome sequences of the RSA MWMV isolates were found to be 9719 nucleotides long, excluding the poly(A) tail. Sequence homology, genetic distances, and phylogenetic analyses indicated close relationships between the RSA isolates. This record will contribute to building up the MWMV isolate sequences from the different countries where the virus occurs, a useful step toward understanding MWMV evolution.
authors.affiliation: Department of Plant Pathology, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Scottsville, Pietermaritzburg, South Africa.
authors.forename: Jacques Davy
authors.initials: JD
authors.lastname: Ibaba
authors.affiliation: Department of Plant Pathology, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Scottsville, Pietermaritzburg, South Africa.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Laing
authors.affiliation: Department of Plant Pathology, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Scottsville, Pietermaritzburg, South Africa. gubbaa@ukzn.ac.za.
authors.forename: Augustine
authors.initials: A
authors.lastname: Gubba
mesh_terms: D019295:Computational Biology; D028464:Cucurbita / Q000821:virology*; D016679:Genome, Viral; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D009276:Namibia; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000145:classification* / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral
keywords: Cucurbit; Next-generation sequencing; Phylogenomics; Potyvirus; Southern Africa; ssRNA virus