title: An improvement of surfactin production by B. subtilis BBG131 using design of experiments in microbioreactors and continuous process in bubbleless membrane bioreactor.
abstract: Culture medium elements were analysed by a screening DoE to identify their influence in surfactin specific production by a surfactin constitutive overproducing Bacillus subtilis strain. Statistics pointed the major enhancement caused by high glutamic acid concentrations, as well as a minor positive influence of tryptophan and glucose. Successively, a central composite design was performed in microplate bioreactors using a BioLector®, in which variations of these impressive parameters, glucose, glutamic acid and tryptophan concentrations were selected for optimization of product-biomass yield (YP/X). Results were exploited in combination with a RSM. In absolute terms, experiments attained an YP/X 3.28-fold higher than those obtained in Landy medium, a usual culture medium used for lipopeptide production by B. subtilis. Therefore, two medium compositions for enhancing biomass and surfactin specific production were proposed and tested in continuous regime in a bubbleless membrane bioreactor. An YP/X increase of 2.26-fold was observed in bioreactor scale.
authors.affiliation: Univ-Lille, EA 7394, Research Institute for Food and Biotechnology - Charles Viollette - Team ProBioGEM, F-59000 Lille, France; L'Oréal Research & Innovation, Advanced Research, Aulnay-sous-Bois, France.
authors.forename: Luiz Fernando
authors.initials: LF
authors.lastname: Motta Dos Santos
authors.affiliation: Univ-Lille, EA 7394, Research Institute for Food and Biotechnology - Charles Viollette - Team ProBioGEM, F-59000 Lille, France. Electronic address: francois.coutte@polytech-lille.fr.
authors.forename: François
authors.initials: F
authors.lastname: Coutte
authors.affiliation: Univ-Lille, EA 7394, Research Institute for Food and Biotechnology - Charles Viollette - Team ProBioGEM, F-59000 Lille, France.
authors.forename: Rozenn
authors.initials: R
authors.lastname: Ravallec
authors.affiliation: Univ-Lille, EA 7394, Research Institute for Food and Biotechnology - Charles Viollette - Team ProBioGEM, F-59000 Lille, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Dhulster
authors.affiliation: L'Oréal Research & Innovation, Advanced Research, Aulnay-sous-Bois, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Tournier-Couturier
authors.affiliation: Univ-Lille, EA 7394, Research Institute for Food and Biotechnology - Charles Viollette - Team ProBioGEM, F-59000 Lille, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Jacques
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry; D055666:Lipopeptides / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*
chemical_list: D003470:Culture Media; D055666:Lipopeptides; D010456:Peptides, Cyclic; C009365:surfactin peptide
keywords: B. subtilis; Design of experiments; Medium optimization; Response surface methodology; Surfactin