title: Epimeric spirolactone-type triterpenoids from Abies faxoniana Rehd.
abstract: Phytochemical investigation of Abies faxoniana Rehd. led to the isolation of two pairs of new epimeric spirolactone-type triterpenoids (1/1' and 2/2') and 11 known terpenoids (3-13). Compounds 1/1' and 2/2' were isolated as epimeric mixtures due to the C-23 ketal tautomerism in their spirolactone structures. The dynamic HPLC manifested that the C-23 epimeric mixtures interconverted into each other in solution. Structure determinations were based on extensive NMR and HRESIMS spectroscopic analysis. Meanwhile, their cytotoxic activities were tested by MTT method. Compound 5 showed cytotoxicities against MCF-7 and A549 cells with IC50 values of 6.5 and 5.7μM, respectively. Compounds 1/1' had IC50 values of 10.0 and 12.3μM for Huh7 and SMMC7721 cells, respectively.
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Wang
authors.affiliation: Department of Phytochemistry, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lv
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Hui-Zi
authors.initials: HZ
authors.lastname: Jin
authors.affiliation: Department of Phytochemistry, Second Military Medical University, Shanghai 200433, PR China. Electronic address: shenyunheng@hotmail.com.
authors.forename: Yun-Heng
authors.initials: YH
authors.lastname: Shen
authors.affiliation: Department of Phytochemistry, Second Military Medical University, Shanghai 200433, PR China; Shanghai Institute of Pharmaceutical Industry, Shanghai 200400, PR China; School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China. Electronic address: wdzhangy@hotmail.com.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
mesh_terms: D028202:Abies / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D006801:Humans; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry; D013148:Spironolactone / Q000737:chemistry* / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes; D013148:Spironolactone
keywords: Abies faxoniana; Cytotoxicity; Hepatoma cells; Triterpenoids