title: Phenotypic homogeneity with minor deviance in osmotic fragility of Sahel goat erythrocytes in non-ionic sucrose media during various physiologic states.
abstract: BACKGROUND
  Erythrocyte swelling in non-ionic sucrose media and the subsequent osmotic lysis are influenced by mechanisms of regulatory volume adjustment and osmotic water permeability. Kinetics of transmembrane water and ion fluxes in varied physiologic states may determine the phenotype of erythrocyte osmotic fragility (EOF) and affect estimates of EOF.
  
  
  METHODS
  Effects of sex, age, late pregnancy (third trimester) and lactation on the haemolysis of Sahel goat erythrocytes incubated in a series of hyposmotic non-ionic sucrose media were investigated.
  
  
  RESULTS
  The fragiligram was sigmoidal in 72 (97%) out of 74 goats. Two male (3%) goats with low and high extreme median erythrocyte fragilities (MEF), had non-sigmoidal curves. The mean fragilities at osmolarities of 30-300 mosmol/L of sucrose and the mean osmolarities responsible for 10%-90% haemolysis (CH10-CH90) were not significantly different between males and non-pregnant dry (NPD) females, amongst the age groups and between pregnant or lactating and NPD female goats. The MEF (CH50) of the goats were at osmolarities of 126-252 mosmol/L (median of data: 171 mosmol/L) with a mean of 175.24±16.20 mosmol/L. Therefore, phenotypic homogeneity of EOF occurred with minor deviance, since EOF variables were not differentiated by sex, age, late pregnancy or lactation.
  
  
  CONCLUSIONS
  Physiologic states of the goat did not affect EOF phenotype in non-ionic sucrose media. Sigmoidal fragility phenotype seemed to be homogeneously conserved by osmoregulatory mechanisms not partitioned by sex, age, late pregnancy or lactation, but a minor non-sigmoidal curve might have occurred due to altered erythrocyte osmotic behaviour that would require further investigation.
authors.forename: Nanacha Afifi
authors.identifier: http://orcid.org/0000-0002-0564-6113
authors.initials: NA
authors.lastname: Igbokwe
authors.forename: Ikechukwu Onyebuchi
authors.initials: IO
authors.lastname: Igbokwe
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000502:physiology*; D005260:Female; D006041:Goats; D006461:Hemolysis / Q000187:drug effects / Q000502:physiology; D008297:Male; D009996:Osmotic Fragility / Q000187:drug effects* / Q000502:physiology*; D010641:Phenotype; D011247:Pregnancy; D013395:Sucrose / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D013395:Sucrose