title: Immunophenotype of Abnormal Metaphases Demonstrating Multilineage Involvement in Myelodysplastic Syndromes.
abstract: Eight patients with myelodysplastic syndrome (MDS) and chromosome abnormalities were studied by a combined method which allows simultaneous analysis of the karyotype and immunophenotype of the same mitotic cell. To determine the cell lineages with abnormal karyotype, monoclonal antibodies in the alkaline phosphatase-antialkaline phosphatase (APAAP) staining methods were used All patients with MDS showed metaphases with abnormal karyotype in cells belonging to granulocytic/monocytic lineage. In 7 patients studied the leukaemic process also involved erythrocytic and/or megakaryocytic lineages. In 4 patients with 5q- chromosome it was evident that at least the granulocytic/monocytic and erythrocytic cell lineages participated in the clonal proliferation. One patient with 5q- chromosome at initial diagnosis showed a shift from megakaryocytic to a granulocytic/monocytic cell population at the time of acute leukaemia These findings indicate multi-lineage involvement in patients with MDS who have karyo-typic abnormalities.
authors.affiliation: a Medical University Clinic, Department of Oncology and Haematology, Hamberg, West Germany.
authors.forename: S
authors.initials: S
authors.lastname: Suciu
authors.affiliation: a Medical University Clinic, Department of Oncology and Haematology, Hamberg, West Germany.
authors.forename: W
authors.initials: W
authors.lastname: Zeller
authors.affiliation: a Medical University Clinic, Department of Oncology and Haematology, Hamberg, West Germany.
authors.forename: W
authors.initials: W
authors.lastname: Fiedler
authors.affiliation: a Medical University Clinic, Department of Oncology and Haematology, Hamberg, West Germany.
authors.forename: H J
authors.initials: HJ
authors.lastname: Weh
authors.affiliation: a Medical University Clinic, Department of Oncology and Haematology, Hamberg, West Germany.
authors.forename: D K
authors.initials: DK
authors.lastname: Hossfeld
keywords: APAAP-stained metaphases; MDS; cell lineage involvement