title: Infection of laboratory colonies of Anopheles mosquitoes with Plasmodium vivax from cryopreserved clinical isolates.
abstract: Plasmodium vivax is the most geographically widespread malaria parasite. Unique features of transmission biology complicate P. vivax control. Interventions targeting transmission are required for malaria eradication. In the absence of an in vitro culture, transmission studies rely on live isolates from non-human primates or endemic regions. Here, we demonstrate P. vivax gametocytes from both India and Brazil are stable during cryopreservation. Importantly, cryopreserved gametocytes from Brazil were capable of infecting three anopheline mosquito species in feedings done in the United States. These findings create new opportunities for transmission studies in diverse locales.
authors.affiliation: Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Shaw-Saliba
authors.affiliation: Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
authors.forename: David
authors.initials: D
authors.lastname: Clarke
authors.affiliation: Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
authors.forename: Jorge M
authors.initials: JM
authors.lastname: Santos
authors.affiliation: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Menezes
authors.affiliation: Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
authors.forename: Caeul
authors.initials: C
authors.lastname: Lim
authors.affiliation: Department of Chemistry, University of Washington, Seattle, WA, USA; Department of Medicine, Goa Medical College and Hospital, Bambolim 403202, Goa, India.
authors.forename: Anjali
authors.initials: A
authors.lastname: Mascarenhas
authors.affiliation: Department of Chemistry, University of Washington, Seattle, WA, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Chery
authors.affiliation: Department of Medicine, Goa Medical College and Hospital, Bambolim 403202, Goa, India.
authors.forename: Edwin
authors.initials: E
authors.lastname: Gomes
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors.forename: Sandra
authors.initials: S
authors.lastname: March
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors.forename: Sangeeta N
authors.initials: SN
authors.lastname: Bhatia
authors.affiliation: Department of Chemistry, University of Washington, Seattle, WA, USA; Department of Medicine, Goa Medical College and Hospital, Bambolim 403202, Goa, India.
authors.forename: Pradipsinh K
authors.initials: PK
authors.lastname: Rathod
authors.affiliation: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Marcelo U
authors.initials: MU
authors.lastname: Ferreira
authors.affiliation: Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
authors.forename: Flaminia
authors.initials: F
authors.lastname: Catteruccia
authors.affiliation: Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA. Electronic address: mduraisi@hsph.harvard.edu.
authors.forename: Manoj T
authors.initials: MT
authors.lastname: Duraisingh
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D001938:Brazil; D015925:Cryopreservation; D006801:Humans; D007194:India; D007303:Insect Vectors / Q000469:parasitology*; D016780:Malaria, Vivax / Q000469:parasitology* / Q000635:transmission; D010966:Plasmodium vivax / Q000502:physiology*
keywords: Anopheles; Cryopreservation; Gametocyte; Liver; Malaria; Plasmodium vivax; Sporozoite; Transmission