title: Simultaneous nano-tracking of multiple motor proteins via spectral discrimination of quantum dots.
abstract: Simultaneous nanometric tracking of multiple motor proteins was achieved by combining multicolor fluorescent labeling of target proteins and imaging spectroscopy, revealing dynamic behaviors of multiple motor proteins at the sub-diffraction-limit scale. Using quantum dot probes of distinct colors, we experimentally verified the localization precision to be a few nanometers at temporal resolution of 30 ms or faster. One-dimensional processive movement of two heads of a single myosin molecule and multiple myosin molecules was successfully traced. Furthermore, the system was modified for two-dimensional measurement and applied to tracking of multiple myosin molecules. Our approach is useful for investigating cooperative movement of proteins in supramolecular nanomachinery.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Laboratory for Comprehensive Bioimaging, RIKEN Quantitative Biology Center, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan.
authors.forename: Taishi
authors.initials: T
authors.lastname: Kakizuka
authors.affiliation: Department of Advanced Materials Science, Graduate School of Frontier Sciences, the University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa City, Chiba 277-8561, Japan.
authors.forename: Keigo
authors.initials: K
authors.lastname: Ikezaki
authors.affiliation: Laboratory for Comprehensive Bioimaging, RIKEN Quantitative Biology Center, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Kaneshiro
authors.affiliation: Laboratory for Comprehensive Bioimaging, RIKEN Quantitative Biology Center, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan; World Premier International Research Center Initiative, Immunology Frontier Research Center, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Fujita
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Laboratory for Comprehensive Bioimaging, RIKEN Quantitative Biology Center, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan; World Premier International Research Center Initiative, Immunology Frontier Research Center, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Tomonobu M
authors.initials: TM
authors.lastname: Watanabe
authors.affiliation: Laboratory for Comprehensive Bioimaging, RIKEN Quantitative Biology Center, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Ichimura
keywords: (100.6640) Superresolution; (110.4234) Multispectral and hyperspectral imaging; (170.3880) Medical and biological imaging; (180.2520) Fluorescence microscopy; (300.6280) Spectroscopy, fluorescence and luminescence