title: Proteomics of hot-wet and cold-dry temperaments proposed in Iranian traditional medicine: a Network-based Study.
abstract: Lack of molecular biology evidence has led clinical success of alternative and complementary medicine (CAM) to be marginalized. In turn, a large portion of life Science researchers could not communicate and help to develop therapeutic potential laid in these therapeutic approaches. In this study, we began to quantify descriptive classification theory in one of the CAM branches i.e. Iranian traditional medicine (ITM). Using proteomic tools and network analysis, the expressed proteins and their relationships were studied in mitochondrial lysate isolated from PBMCs from two different temperaments i.e. Hot-wet (HW) and Cold-dry (CD). The 82% of the identified proteins are over- or under-represented in distinct temperaments. Also, our result showed the different protein-protein interaction networks (PPIN) represented in these two temperaments using centrality and module finding analysis. Following the gene ontology and pathway enrichment analysis, we have found enriched biological terms in each group which are in conformity with the physiologically known evidence in ITM. In conclusion, we argued that the network biology which naturally consider life at the system level along with the different omics data will pave the way toward explicit delineation of the CAM activities.
authors.affiliation: Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Rezadoost
authors.affiliation: Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran.|School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Karimi
authors.affiliation: Drug Design and Bioinformatics Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 131694-3551, Iran.
authors.forename: Mohieddin
authors.initials: M
authors.lastname: Jafari
mesh_terms: D002478:Cells, Cultured; D003080:Cold Temperature; D053263:Gene Regulatory Networks / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D007492:Iran; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008519:Medicine, Traditional / Q000379:methods; D060066:Protein Interaction Maps / Q000502:physiology*; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D011506:Proteins