title: Reclassification of Eubacterium desmolans as Butyricicoccus desmolans comb. nov., and description of Butyricicoccus faecihominis sp. nov., a butyrate-producing bacterium from human faeces.
abstract: A Gram-positive-staining, coccoid-shaped, non-motile, asporogenous, obligately anaerobic and butyrate-producing bacterium was recovered from a healthy human's faeces. The organism was isolated by the enrichment culture technique using yeast extract-casein hydrolysate-fatty acids broth supplemented with 0.5 % mucin. Phylogenetic analysis of 16S rRNA gene sequences demonstrated that the novel strain should be classified as a member of the Eubacterium desmolans-related cluster in the family Ruminococcaceae. Furthermore, this analysis demonstrated that the type strains of Butyricicoccus pullicaecorum (95.6 %) and Eubacterium desmolans (94.7 %) were the closest phylogenetic neighbours to strain YIT 12789T. However, DNA‒DNA reassociation values with these closest strains were less than 20 %. On the basis of the phenotypic, genotypic and chemotaxonomic features, the novel coccoid-shaped bacterium should be designated as a representative of a novel species of the genus Butyricicoccus, for which the name Butyricicoccus faecihominis sp. nov. is proposed. The type strain is YIT 12789T (=JCM 31056T=DSM 100989T). It is also proposed that Eubacterium desmolans be reclassified in the genus Butyricicoccus as Butyricicoccus desmolans comb. nov.
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi-shi, Tokyo 186-8650, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Takada
authors.affiliation: Department of Animal Science and Technology, National Taiwan University, No. 50 Lane 155, Sec 3, Keelung Rd, Taipei 10673, Taiwan, ROC.
authors.forename: Koichi
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi-shi, Tokyo 186-8650, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Makino
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi-shi, Tokyo 186-8650, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kushiro
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002087:Butyrates / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005051:Eubacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D002087:Butyrates; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S