title: Genomic, transcriptomic and proteomic analyses of Dehalobacter UNSWDHB in response to chloroform.
abstract: Organohalide respiring bacteria (ORB) are capable of utilising organohalides as electron acceptors for the generation of cellular energy and consequently play an important role in the turnover of natural and anthropogenically-derived organohalides. In this study, the response of a Dehalobacter sp. strain UNSWDHB to the addition of trichloromethane (TCM) after a 50 h period of its absence (suffocation) was evaluated from a transcriptomic and proteomic perspective. The up-regulation of TCM reductive dehalogenase genes (tmrABC) and their gene products (TmrABC) was confirmed at both transcriptional and proteomic levels. Other findings include the upregulation of various hydrogenases (membrane-associated Ni-Fe hydrogenase complexes and soluble Fe-Fe hydrogenases), formate dehydrogenases, complex I and a pyrophosphate-energized proton pump. The elevated expression of enzymes associated with carbon metabolism, including complete Wood Ljungdahl pathway, during TCM respiration raises interesting questions on possible fates of intracellular formate and its potential role in the physiology of this bacterium. Overall, the findings presented here provide a broader view on the bioenergetics and general physiology of Dehalobacter UNSWDHB cells actively respiring with TCM.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Bat-Erdene
authors.initials: BE
authors.lastname: Jugder
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, 2052, Australia.|Department of Molecular Biology and Genetics, Istanbul University, Turkey.
authors.forename: Haluk
authors.initials: H
authors.lastname: Ertan
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Yie Kuan
authors.initials: YK
authors.lastname: Wong
authors.affiliation: Faculty of Medicine, School of Psychiatry, Centre for Healthy Brain Ageing, University of New South Wales, Sydney, Australia.
authors.forename: Nady
authors.initials: N
authors.lastname: Braidy
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: Manefield
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Marquis
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Matthew
authors.initials: M
authors.lastname: Lee