title: Development of microsatellite markers for Crepis mollis (Asteraceae).
abstract: OBJECTIVE
  Polymorphic microsatellite markers were developed for the threatened species Crepis mollis (Asteraceae) to investigate population and conservation genetics.
  
  
  RESULTS
  Illumina sequencing was conducted on pooled genomic DNA from 10 individuals of two populations. Ten polymorphic and 10 monomorphic microsatellite loci with di-, tri-, tetra-, penta-, and hexanucleotide repeat motifs were developed and characterized in C. mollis. In the polymorphic markers, up to 17 alleles per locus were detected with an observed and expected heterozygosity ranging from 0.120 to 0.780 and 0.102 to 0.834, respectively. Furthermore, the polymorphic markers were tested for cross-amplification in three congeneric species (C. biennis, C. foetida, and C. sancta) and amplified in up to three loci.
  
  
  CONCLUSIONS
  The markers developed in this study are the first microsatellites tested on C. mollis and will be useful for performing population and conservation genetic studies in this threatened species.
authors.affiliation: Botanischer Garten und Botanisches Museum Berlin-Dahlem, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Königin Luise-Straße 6-8, 14195 Berlin, Germany.
authors.forename: Virginia K
authors.initials: VK
authors.lastname: Duwe
authors.affiliation: Institut für Biologie-Botanik, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Altensteinstraße 6, 14195 Berlin, Germany.
authors.forename: Ludo A H
authors.initials: LA
authors.lastname: Muller
authors.affiliation: Botanischer Garten und Botanisches Museum Berlin-Dahlem, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Königin Luise-Straße 6-8, 14195 Berlin, Germany; Institut für Biologie-Botanik, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Altensteinstraße 6, 14195 Berlin, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Borsch
authors.affiliation: Botanischer Garten und Botanisches Museum Berlin-Dahlem, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Königin Luise-Straße 6-8, 14195 Berlin, Germany; School of Biological Sciences, University of Aberdeen, 23 St. Machar Drive, Aberdeen, AB24 3UU Scotland.
authors.forename: Sascha A
authors.initials: SA
authors.lastname: Ismail
keywords: Asteraceae; Crepis mollis; Illumina; microsatellites; population genetics