title: Targeted Delivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells.
abstract: The application of homing peptides to direct DNA and RNA lipoplexes to target cells is a rapidly evolving area of study, which may find application in corrective gene therapy for the treatment of neoplasms and other disorders of a genetic origin. Here, a step-wise account of the assembly and characterization of hepatocellular carcinoma cell-specific DNA lipoplexes and their cytotoxicity assessment in and delivery to the human hepatocellular carcinoma cell line HepG2 is given.
authors.affiliation: Discipline of Biochemistry, University of KwaZulu-Natal, Westville Campus, P. Bag X54001, Durban, 4000, South Africa. ariattim@ukzn.ac.za.
authors.forename: Mario
authors.initials: M
authors.lastname: Ariatti
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000628:therapy; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000235:genetics* / Q000494:pharmacology; D015316:Genetic Therapy; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D008081:Liposomes; D008113:Liver Neoplasms / Q000235:genetics* / Q000628:therapy; D058990:Molecular Targeted Therapy; D010455:Peptides / Q000235:genetics*; D014162:Transfection
chemical_list: D008081:Liposomes; D010455:Peptides; D004247:DNA
keywords: Gene delivery; Hepatocellular carcinoma; Homing-peptide; Lipoplex; Targeting