title: The long noncoding RNA MALAT1 regulates the lipopolysaccharide-induced inflammatory response through its interaction with NF-κB.
abstract: MALAT1 is a conserved long noncoding RNA whose expression correlates with many human cancers. However, its significance in immunity remains largely unknown. Here, we observe that MALAT1 is upregulated in lipopolysaccharide (LPS)-activated macrophages. Knockdown of MALAT1 increases LPS-induced expression of TNFα and IL-6. Mechanistically, MALAT1 was found to interact with NF-κB in the nucleus, thus inhibiting its DNA binding activity and consequently decreasing the production of inflammatory cytokines. Additionally, abnormal expression of MALAT1 was found to be NF-κB-dependent. These findings suggest that MALAT1 may function as an autonegative feedback regulator of NF-κB to help fine-tune innate immune responses.
authors.affiliation: Department of Biochemistry, School of Medicine & Key Laboratory of Ministry of Education for Developmental Genes and Human Diseases, Southeast University, Nanjing, China.
authors.forename: Gui
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Department of Biochemistry, School of Medicine & Key Laboratory of Ministry of Education for Developmental Genes and Human Diseases, Southeast University, Nanjing, China.
authors.forename: Zhenyi
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Biochemistry, School of Medicine & Key Laboratory of Ministry of Education for Developmental Genes and Human Diseases, Southeast University, Nanjing, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Song
authors.affiliation: Department of Biochemistry, School of Medicine & Key Laboratory of Ministry of Education for Developmental Genes and Human Diseases, Southeast University, Nanjing, China.|School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Department of Biochemistry, School of Medicine & Key Laboratory of Ministry of Education for Developmental Genes and Human Diseases, Southeast University, Nanjing, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Mao
mesh_terms: D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; C557041:MALAT1 long non-coding RNA, human; D016328:NF-kappa B; C498143:RELA protein, human; D062085:RNA, Long Noncoding; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha
keywords: MALAT1; Macrophage; NF-κB transcription factor; inflammation; innate immunity