title: A Chemical Genetic Screening Procedure forArabidopsis thalianaSeedlings.
abstract: Unbiased screening approaches are powerful tools enabling identification of novel players in biological processes. Chemical genetic screening refers to the technique of using a reporter response, such as expression of luciferase driven by a promoter of interest, to discover small molecules that affect a given process when applied to plants. These chemicals then act as tools for identification of regulatory components that could not otherwise be detected by forward genetic screens due to gene family redundancy or mutant lethality. This protocol describes a chemical genetic screen usingArabidopsis thalianaseedlings, which has led to recognition of novel players in the plant general stress response.
authors.affiliation: Department of Plant Sciences, University of California, Davis, USA; Department of Plant Sciences, University of California, Davis, USA.
authors.forename: Marta
authors.initials: M
authors.lastname: Bjornson
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, China.
authors.forename: Xingshun
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Plant Sciences, University of California, Davis, USA.
authors.forename: Abhaya
authors.initials: A
authors.lastname: Dandekar
authors.affiliation: Department of Chemistry, University of California, Davis, USA.
authors.forename: Annaliese
authors.initials: A
authors.lastname: Franz
authors.affiliation: Department of Plant Sciences, University of California, Davis, USA.
authors.forename: Georgia
authors.initials: G
authors.lastname: Drakakaki
authors.affiliation: Department of Plant Sciences, University of California, Davis, USA.
authors.forename: Katayoon
authors.initials: K
authors.lastname: Dehesh