title: Upregulated long noncoding RNA SPRY4-IT1 contributes to increased cell viability by activating zinc finger 703 expression in esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  The function of long noncoding RNA SPRY4-IT1 in human esophageal squamous cell carcinoma (ESCC) has been showed in the former studies. The purpose of this study was to further analyze the underlined mechanisms responsible for its role in ESCC cells.
  
  
  METHODS
  Quantitative reverse transcriptase polymerase chain reaction was firstly used to measure the expression of SPRY4-IT1 in 50 ESCC patients of different clinical stages. Loss of function approach was then applied to confirm the biological function, especially cell viabilities in cultured ESCC cells, by cell counting kit-8 and clonogenic assay. We further used western blot to reveal the activation of zinc finger 703 (ZNF703) by SPRY4-IT1.
  
  
  RESULTS
  We validated that SPRY4-IT1 was upregulated in ESCC tissues of advanced clinical stages. In vitro function assays demonstrated that SPRY4-IT1 cause promotion of cell viability in ESCC cells. We further verified that SPRY4-IT1 could also activate the expression of ZNF703 in ESCC cells, which might contribute to the role of SPRY4-IT1 in ESCC cells.
  
  
  CONCLUSIONS
  SPRY4-IT1 is a vital regulator in ESCC progression, and the SPRY4-IT1/ZNF703 axis might provide novel clues for future ESCC therapy.
authors.affiliation: Department of Gastroenterology, Lishui People's Hospital, Lishui 323000, Zhejiang Province, PR China.
authors.forename: J
authors.initials: J
authors.lastname: Xue-Liang
authors.affiliation: Department of Clinical Pharmacy, Lishui People's Hospital, Lishui 323000, Zhejiang Province, PR China.
authors.forename: W
authors.initials: W
authors.lastname: Ming-Dong
authors.affiliation: Department of Gastroenterology, Lishui People's Hospital, Lishui 323000, Zhejiang Province, PR China.
authors.forename: Z
authors.initials: Z
authors.lastname: Ya-Bi
authors.affiliation: Department of Gastroenterology, Lishui People's Hospital, Lishui 323000, Zhejiang Province, PR China.
authors.forename: W
authors.initials: W
authors.lastname: Wang-Yue
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D006801:Humans; D009367:Neoplasm Staging; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection; D015854:Up-Regulation; D016335:Zinc Fingers
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D062085:RNA, Long Noncoding; C541016:ZNF703 protein, human; C000605823:long noncoding RNA SPRY4-IT1, human