title: Hydrogen isotopes in individual amino acids reflect differentiated pools of hydrogen from food and water in Escherichia coli.
abstract: Hydrogen isotope (δ(2)H) analysis is widely used in animal ecology to study continental-scale movement because δ(2)H can trace precipitation and climate. To understand the biochemical underpinnings of how hydrogen is incorporated into biomolecules, we measured the δ(2)H of individual amino acids (AAs) in Escherichia coli cultured in glucose-based or complex tryptone-based media in waters with δ(2)H values ranging from -55‰ to +1,070‰. The δ(2)H values of AAs in tryptone spanned a range of ∼250‰. In E. coli grown on glucose, the range of δ(2)H among AAs was nearly 200‰. The relative distributions of δ(2)H of AAs were upheld in cultures grown in enriched waters. In E. coli grown on tryptone, the δ(2)H of nonessential AAs varied linearly with the δ(2)H of media water, whereas δ(2)H of essential AAs was nearly identical to δ(2)H in diet. Model calculations determined that as much as 46% of hydrogen in some nonessential AAs originated from water, whereas no more than 12% of hydrogen in essential AAs originated from water. These findings demonstrate that δ(2)H can route directly at the molecular level. We conclude that the patterns and distributions in δ(2)H of AAs are determined through biosynthetic reactions, suggesting that δ(2)H could become a new biosignature for studying novel microbial pathways. Our results also show that δ(2)H of AAs in an organism's tissues provides a dual tracer for food and environmental (e.g., drinking) water.
authors.affiliation: Geophysical Laboratory, Carnegie Institution of Washington, Washington, DC 20015; Life and Environmental Sciences, School of Natural Science, University of California, Merced, CA 95343; mfogel@ucmerced.edu.
authors.forename: Marilyn L
authors.initials: ML
authors.lastname: Fogel
authors.affiliation: Geophysical Laboratory, Carnegie Institution of Washington, Washington, DC 20015; Department of Geological Sciences, Indiana University, Bloomington, IN 47408;
authors.forename: Patrick L
authors.initials: PL
authors.lastname: Griffin
authors.affiliation: Geophysical Laboratory, Carnegie Institution of Washington, Washington, DC 20015; Department of Biology, University of New Mexico, Albuquerque, NM 87131.
authors.forename: Seth D
authors.initials: SD
authors.lastname: Newsome
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D003903:Deuterium; D004463:Ecology; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005504:Food Analysis; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000032:analysis*; D014867:Water / Q000032:analysis*
chemical_list: D000596:Amino Acids; D014867:Water; D006859:Hydrogen; D003903:Deuterium; D005947:Glucose
keywords: Escherichia coli; aminio acids; diet; hydrogen isotopes