title: Serum human chorionic gonadotropin measured 7 days following day 3 embryo transfer might predict pregnancy outcome in IVF.
abstract: This prospective study investigated the predictive value of pregnancy outcomes with serum human chorionic gonadotropin (hCG) 7 days after day 3 embryo transfer (D3 ET), and whether estradiol (E2) and progesterone (P) improved the diagnostic efficiency. The study comprised 280 in vitro fertilization and embryo transfer (IVF-ET) cycles. Serum samples were obtained 7 days after D3 ET to measure hCG, E2, and P concentrations. Statistical analyses were conducted to evaluate the predictive value for pregnancy outcomes. We found significant differences in hCG level between pregnancy and non-pregnancy, viable and non-viable pregnancy, biochemical and viable pregnancy, as well as singleton and multiple pregnancy. An hCG cutoff value of 2.5 mIU/mL is predictive of pregnancy with a positive predictive value (PPV) of 95.9% and a negative predictive value (NPV) of 92.4%. An hCG value of 10.8 mIU/mL is predictive of a multiple pregnancy with an NPV of 98.1%. The area under the hCG curve between pregnancy and non-pregnancy was not improved by adding E2, P, or combined E2/P. Our results suggest a predictive value of pregnancy outcome with serum hCG drawn 7 days after D3 ET in IVF, and the diagnostic accuracy is not improved by adding measurements of E2/P.
authors.affiliation: a Department of Obstetrics and Gynecology , Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.|b Key Laboratory for Reproductive Medicine of Guangdong , Guangzhou , China , and.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: a Department of Obstetrics and Gynecology , Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.
authors.forename: Ruixiao
authors.initials: R
authors.lastname: Zhang
authors.affiliation: a Department of Obstetrics and Gynecology , Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.
authors.forename: Mengxi
authors.initials: M
authors.lastname: Jia
authors.affiliation: a Department of Obstetrics and Gynecology , Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.|b Key Laboratory for Reproductive Medicine of Guangdong , Guangzhou , China , and.
authors.forename: Lu
authors.initials: L
authors.lastname: Luo
authors.affiliation: a Department of Obstetrics and Gynecology , Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.|b Key Laboratory for Reproductive Medicine of Guangdong , Guangzhou , China , and.
authors.forename: Chenhui
authors.initials: C
authors.lastname: Ding
authors.affiliation: c Department of Obstetrics & Gynecology , Prince of Wales Hospital, Chinese University of Hong Kong , ShaTin, Hong Kong , China.
authors.forename: Tin-Chiu
authors.initials: TC
authors.lastname: Li
authors.affiliation: a Department of Obstetrics and Gynecology , Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.|b Key Laboratory for Reproductive Medicine of Guangdong , Guangzhou , China , and.
authors.forename: Canquan
authors.initials: C
authors.lastname: Zhou
mesh_terms: D000328:Adult; D006063:Chorionic Gonadotropin / Q000097:blood*; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D017063:Outcome Assessment, Health Care; D011247:Pregnancy; D011272:Pregnancy, Multiple; D013997:Time Factors
chemical_list: D006063:Chorionic Gonadotropin
keywords: In vitro fertilization; pregnancy outcome; serum estradiol; serum hCG; serum progesterone