title: Extracellular Recordings of Patterned Human Pluripotent Stem Cell-Derived Cardiomyocytes on Aligned Fibers.
abstract: Human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) hold high potential for use in drug assessment and myocardial regeneration. To create tissue-like constructs of CMs for extracellular monitoring, we placed aligned fibers (AFs) on the surface of a microelectrode array and then seeded hiPSC-CMs for subsequent monitoring for 14 days. As expected, the CMs organized into anisotropic and matured tissue and the extracellular recordings showed reduced premature beating higher signal amplitude and a higher probability of T-wave detection as compared to the culture without fibers. The CMs on the aligned fibers samples also exhibited anisotropic propagation of the field potential. These results therefore suggest that the hiPSC-CMs cultured on AFs can be used more reliably for cell based assays.
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Junjun
authors.identifier: 0000-0002-7473-4918
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Itsunari
authors.initials: I
authors.lastname: Minami
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto 606-8501, Japan; Department of Micro Engineering, Kyoto University, Katsura, Nishi-ku, Kyoto 615-8540, Japan.
authors.forename: Leqian
authors.initials: L
authors.lastname: Yu
authors.affiliation: Bio Research Department, Device Research Laboratory, Advanced Research Division, Panasonic Corporation, 3-4 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan.
authors.forename: Kiyotaka
authors.identifier: 0000-0002-6712-5989
authors.initials: K
authors.lastname: Tsuji
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto 606-8501, Japan; Department of Micro Engineering, Kyoto University, Katsura, Nishi-ku, Kyoto 615-8540, Japan.
authors.forename: Minako
authors.initials: M
authors.lastname: Nakajima
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Jing
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Bio Research Department, Device Research Laboratory, Advanced Research Division, Panasonic Corporation, 3-4 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan.
authors.forename: Masato
authors.identifier: 0000-0003-2040-3029
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Bio Research Department, Device Research Laboratory, Advanced Research Division, Panasonic Corporation, 3-4 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Shimono
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Nakatsuji
authors.affiliation: Department of Micro Engineering, Kyoto University, Katsura, Nishi-ku, Kyoto 615-8540, Japan.
authors.forename: Hitetoshi
authors.initials: H
authors.lastname: Kotera
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Li
authors.identifier: 0000-0002-4854-3886
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto 606-8501, Japan; Ecole Normale Supérieure, CNRS-ENS-UPMC UMR 8640, 24 Rue Lhomond, 75005 Paris, France.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen