title: Rational Molecular Design of Potent PLK1 PBD Domain-binding Phosphopeptides Using Preferential Amino Acid Building Blocks.
abstract: Polo-like kinase 1 (PLK1) is an important regulator in diverse aspects of the cell cycle and proliferation. The protein has a highly conserved polo-box domain (PBD) present in C-terminal noncatalytic region, which exhibits a relatively broad sequence specificity in recognizing and binding phosphorylated substrates to control substrate phosphorylation by the kinase. In order to elucidate the structural basis, thermodynamic property, and biological implication underlying PBD-substrate recognition and association, a systematic amino acid preference profile of phosphopeptide interaction with PLK1 PBD domain was established via virtual mutagenesis analysis and mutation energy calculation, from which the contribution of different amino acids at each residue position of two reference phosphopeptides to domain-peptide binding was characterized comprehensively and quantitatively. With the profile, we are able to determine the favorable, neutral, and unfavorable amino acid types for each position of PBD-binding phosphopeptides, and we also explored the molecular origin of the broad sequence specificity in PBD-substrate recognition. To practice computational findings, the profile was further employed to guide rational design of potent PBD binders; three 6-mer phosphopeptides (i.e., IQSpSPC, LQSpTPF, and LNSpTPT) were successfully developed, which can efficiently target PBD domain with high affinity (Kd = 5.7 ± 1.1, 0.75 ± 0.18, and 7.2 ± 2.6 μm, resp.) as measured by a fluorescence anisotropy assay. The complex structure of PLK1 PBD domain with a newly designed, potent phosphopeptide LQSpTPF as well as diverse noncovalent chemical forces, such as H-bonds and hydrophobic interactions at the complex interface, were examined in detail to reveal the molecular mechanism of high affinity and stability of the complex system.
authors.affiliation: Department of Gastroenterology, Taizhou Hospital of Zhejiang Province, Linhai, 317000, P. R. China.
authors.forename: Xin-Li
authors.initials: XL
authors.lastname: Mao
authors.affiliation: Department of Infectious Disease, Taizhou Hospital of Zhejiang Province, Linhai, 317000, P. R. China.
authors.forename: Kui-Feng
authors.initials: KF
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Taizhou Hospital of Zhejiang Province, Linhai, 317000, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Otorhinolaryngology, Taizhou Hospital of Zhejiang Province, No. 150 Ximen Str., Linhai, 317000, P. R. China.
authors.forename: Zhao-Hu
authors.initials: ZH
authors.lastname: Pan
authors.affiliation: Department of Otorhinolaryngology, Taizhou Hospital of Zhejiang Province, No. 150 Ximen Str., Linhai, 317000, P. R. China.
authors.forename: Guo-Min
authors.initials: GM
authors.lastname: Wu
authors.affiliation: Department of Otorhinolaryngology, Taizhou Hospital of Zhejiang Province, No. 150 Ximen Str., Linhai, 317000, P. R. China. zhuhy9@163.com.
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Zhu
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D010748:Phosphopeptides / Q000737:chemistry* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics; D000097869:Polo-Like Kinase 1
chemical_list: D000596:Amino Acids; D018797:Cell Cycle Proteins; D010748:Phosphopeptides; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1
keywords: Building block; Domain-peptide interaction; Phosphopeptide; Polo-like kinase 1; Rational molecular design