title: Component-resolved diagnostics in vernal conjunctivitis.
abstract: Conventional diagnostic tests in allergy are insufficient to clarify the cause of vernal conjunctivitis. Component-resolved diagnostic (CRD) by microarray allergen assay may be useful in detecting allergens that might be involved in the inflammatory process.
  
  
  
  In a recent trial in patients suffered from eosinophilic esophagitis, after 2 years of the CRD-guided exclusion diet and specific immunotherapy, significant clinical improvement was observed, and 68% of patients were discharged (cure based on negative biopsy, no symptoms, and no medication intake). Our new objective was to evaluate IgE-mediated hypersensitivity by CRD in tears and serum from patients with vernal conjunctivitis and treat patients with identified triggering allergens by specific immunotherapy. Twenty-five patients with vernal conjunctivitis were evaluated. The identified triggering allergens were n Lol p 1 (11 cases), n Cyn d 1 (eight cases), group 4 and 6 grasses (six cases) and group 5 of grasses (five cases). Prick test and pollen IgE were positive in one case. Clinical improvement was observed in 13/25 vernal conjunctivitis patients after 1-year specific immunotherapy.
  
  
  
  CRD seems to be a more sensitive diagnostic tool compared with prick test and IgE detection. Specific CRD-led immunotherapy may achieve clinical improvements in vernal conjunctivitis patients.
authors.affiliation: aAllergy Unit bOphthalmology Unit, Rio Hortega University Hospital, Valladolid, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Armentia
authors.forename: Eugenia
authors.initials: E
authors.lastname: Sanchís
authors.forename: Javier A
authors.initials: JA
authors.lastname: Montero
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology; D002986:Clinical Trials as Topic; D003233:Conjunctivitis, Allergic / Q000175:diagnosis* / Q000276:immunology / Q000628:therapy; D031744:Cynodon / Q000276:immunology; D003888:Desensitization, Immunologic / Q000379:methods*; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology / Q000628:therapy; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D046228:Microarray Analysis; D010940:Plant Proteins / Q000276:immunology; D011058:Pollen / Q000276:immunology; D012621:Seasons; D013666:Tears / Q000276:immunology*; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C550205:Cyn d 1 allergen, Bermuda grass; C084941:Lol p V protein, Lolium perenne; D010940:Plant Proteins; D007073:Immunoglobulin E