title: Estradiol-tethered micropatterned surfaces for the study of estrogenic non-genomic pathways.
abstract: Besides its well-known hormonal effects initiated in the nucleus, estradiol (E2) also activates non-nuclear pathways through interactions with receptors located on the cell plasma membrane. Micropatterned substrates consisting of gold dots bearing tethered E2 distributed on a cell-adhesive substrate were prepared and shown to trigger specifically E2 non-genomic effects in cells grown on the substrates.
authors.affiliation: Faculté de Pharmacie and Département de Chimie, Université de Montréal, CP 6128 Succursale Center Ville, Montréal, QC H3C 3J7, Canada. francoise.winnik@umontreal.ca.
authors.forename: B
authors.initials: B
authors.lastname: Qi
authors.forename: Y
authors.initials: Y
authors.lastname: Shimizu
authors.forename: J
authors.initials: J
authors.lastname: Nakanishi
authors.forename: F M
authors.initials: FM
authors.lastname: Winnik
mesh_terms: D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000737:chemistry / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D061986:MCF-7 Cells; D010316:Particle Size; D010766:Phosphorylation; D013499:Surface Properties
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D004958:Estradiol; D048049:Extracellular Signal-Regulated MAP Kinases