title: Two New Pentacyclic Triterpene Saponins from the Leaves of Akebia trifoliata.
abstract: Two new pentacyclic triterpene saponins, named akebiaoside K (1) and akebiaoside N (2), were isolated from the leaves of Akebia trifoliata, together with five known triterpenoids 3-7. They were all isolated from the leaves of A. trifoliata for the first time. Their structures were established by spectral and chemical means. Triterpenes 5 and 7 were found to show moderate in vitro cytotoxicity against human tumor A549, HeLa and HepG2 cell lines, with IC50 values ranging from 0.023 to 0.038 mM. Triterpenes 5-7 were further revealed to show significant in vitro α-glucosidase inhibitory activity with IC50 values from 0.040 to 0.220 mM, making them more potent than the reference compound acarbose (IC50 0.409 mM). Meanwhile, no obvious inhibitory effects were observed for the isolated triterpene saponins 1-4 in both bioactivity assays.
authors.affiliation: Guangdong Provincial Key Laboratory of Bio-Control for Forest Diseases and Pests, Guangdong Academy of Forestry, Guangzhou 510520, China. qlxu@sinogaf.cn.
authors.forename: Qiao-Lin
authors.initials: QL
authors.lastname: Xu
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. w_j771@163.com.|Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. w_j771@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. donglimei1990@163.com.|College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. donglimei1990@163.com.
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Dong
authors.affiliation: Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. zqiang55@126.com.|College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. zqiang55@126.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. bluo023@163.com.|College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. bluo023@163.com.
authors.forename: Bi
authors.initials: B
authors.lastname: Luo
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. jyx@scib.ac.cn.|Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. jyx@scib.ac.cn.
authors.forename: Yong-Xia
authors.initials: YX
authors.lastname: Jia
authors.affiliation: Guangdong Provincial Key Laboratory of Bio-Control for Forest Diseases and Pests, Guangdong Academy of Forestry, Guangzhou 510520, China. wanghf@sinogaf.cn.
authors.forename: Hong-Feng
authors.initials: HF
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. jwtan@scbg.ac.cn.|Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. jwtan@scbg.ac.cn.
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Tan
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000737:chemistry*; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry* / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; D014315:Triterpenes
keywords: Akebia trifoliata; cytotoxicity; triterpene saponins; α-glucosidase inhibitor