title: Lipopolysaccharide- and β-1,3-glucan-binding protein from Fenneropenaeus merguiensis functions as a pattern recognition receptor with a broad specificity for diverse pathogens in the defense against microorganisms.
abstract: In crustaceans, lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP) plays an important role in innate immunity by mediating the recognition of pathogens to host cells. Hereby, LGBP was cloned from Fenneropenaeus merguiensis hepatopancreas. Its full-length cDNA (1280 bp) had an open reading frame of 1101 bp, encoding a peptide of 366 amino acids. The LGBP primary structure comprises a recognition motif for β-1,3-linkage of polysaccharides, two integrin binding motifs, a kinase C phosphorylation site and a bacterial glucanase motif. The LGBP mRNA was strongly expressed in hepatopancreas and significantly up-regulated to get the maximum at 12 h upon Vibrio harveyi challenge. Recombinant LGBP (rLGBP) could agglutinate Gram-negative and Gram-positive bacteria including yeast with Ca2+-dependence. V. harveyi agglutination induced by rLGBP was intensively inhibited by lipoteichoic acid, less in order were lipopolysaccharide, β-1,3-glucan and N-acetyl neuraminic acid. Western blotting revealed that rLGBP bound widely to Gram-negative and Gram-positive bacteria and also yeast. By ELISA quantification, rLGBP could bind to β-1,3-glucan better than to lipopolysaccharide and lipoteichoic acid. These findings suggest that LGBP may function as a receptor which recognizes invading diverse pathogens and contribute in F. merguiensis immune response.
authors.affiliation: Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, 90112, Thailand.
authors.forename: Areerat
authors.initials: A
authors.lastname: Chaosomboon
authors.affiliation: Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, 90112, Thailand.
authors.forename: Benjaporn
authors.initials: B
authors.lastname: Phupet
authors.affiliation: Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, 90112, Thailand.
authors.forename: Onnicha
authors.initials: O
authors.lastname: Rattanaporn
authors.affiliation: Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, 90112, Thailand.
authors.forename: Phanthipha
authors.initials: P
authors.lastname: Runsaeng
authors.affiliation: Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, 90112, Thailand. Electronic address: prapaporn.u@psu.ac.th.
authors.forename: Prapaporn
authors.initials: P
authors.lastname: Utarabhand
mesh_terms: D000371:Agglutination; D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D043143:Hepatopancreas / Q000378:metabolism*; D007113:Immunity, Innate; D037102:Lectins / Q000235:genetics; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000276:immunology; D033561:Penaeidae / Q000276:immunology*; D011485:Protein Binding; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000378:metabolism*; D013682:Teichoic Acids / Q000494:pharmacology; D015854:Up-Regulation; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology*; D047071:beta-Glucans / Q000378:metabolism
chemical_list: C403975:LGBP protein, Pacifastacus leniusculus; D037102:Lectins; D008070:Lipopolysaccharides; D000069452:Pathogen-Associated Molecular Pattern Molecules; D051192:Receptors, Pattern Recognition; D013682:Teichoic Acids; D047071:beta-Glucans; C009900:lipoteichoic acid
keywords: Fenneropenaeus merguiensis; Hepatopancreas; Immune response; Lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP); Vibrio harveyi