title: MiR-541-3p reverses cancer progression by directly targeting TGIF2 in non-small cell lung cancer.
abstract: Lung cancer remains a leading cause of cancer-associated mortality worldwide, and non-small lung cancer (NSCLC) is responsible for over 80 % of lung cancer-related deaths. Identifying novel molecular biomarker that can inhibit the progression of lung cancer will facilitate the development of new treatment strategies. Herein, we demonstrated that miR-541-3p is a tumor-suppressor microRNA (miRNA) in NSCLC progression. We found that expression of miR-541-3p was decreased obviously in NSCLC tissues and plasma. Down-regulation of miR-541-3p was associated with TNM stage and postoperative survival. Overexpression of miR-541-3p inhibited the growth and metastasis of NSCLC cells. The TGIF2 was a direct target of miR-541-3p and promoted the growth and metastasis of NSCLC cells. Further study showed that TGIF2 could reverse the inhibitory effect of miR-541-3p on growth and metastasis of NSCLC cells. Taken together, our data highlight the pivotal role of miR-541-3p in the progression of NSCLC. Thus, miR-541-3p may be a potential prognostic marker and of treatment relevance for NSCLC progression intervention.
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230032, Anhui, China.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Lu
authors.affiliation: Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230022, Anhui, China. lryahmu@126.com.
authors.forename: Rong-Yu
authors.initials: RY
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230032, Anhui, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Hu
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230032, Anhui, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000072283:A549 Cells; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018398:Homeodomain Proteins; C000596382:MIRN541 microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins; C414585:TGIF2 protein, human
keywords: NSCLC; Survival; TGIF2; miR-541-3p