title: Metal-linked Immunosorbent Assay (MeLISA): the Enzyme-Free Alternative to ELISA for Biomarker Detection in Serum.
abstract: Determination of disease biomarkers in clinical samples is of crucial significance for disease monitoring and public health. The dominating format is enzyme-linked immunosorbent assay (ELISA), which subtly exploits both the antigen-antibody reaction and biocatalytic property of enzymes. Although enzymes play an important role in this platform, they generally suffer from inferior stability and less tolerant of temperature, pH condition compared with general chemical product. Here, we demonstrate a metal-linked immunosorbent assay (MeLISA) based on a robust signal amplification mechanism that faithfully replaces the essential element of the enzyme. As an enzyme-free alternative to ELISA, this methodology works by the detection of α-fetoprotein (AFP), prostatic specific antigen (PSA) and C-reactive protein (CRP) at concentrations of 0.1 ng mL(-1), 0.1 ng mL(-1) and 1 ng mL(-1) respectively. It exhibits approximately two magnitudes higher sensitivity and is 4 times faster for chromogenic reaction than ELISA. The detection of AFP and PSA was further confirmed by over a hundred serum samples from hepatocellular carcinoma (HCC) and prostate cancer patients respectively.
authors.affiliation: 1. Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, P. R. China.
authors.forename: Ru-Jia
authors.initials: RJ
authors.lastname: Yu
authors.affiliation: 1. Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: 1. Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, P. R. China.
authors.forename: Xiao-Yuan
authors.initials: XY
authors.lastname: Liu
authors.affiliation: 1. Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, P. R. China.
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Jin
authors.affiliation: 2. Translational Medicine Center, Changzheng Hospital, The Second Military Medical University, P. R. China.
authors.forename: Huan-Xing
authors.initials: HX
authors.lastname: Han
authors.affiliation: 3. Laboratory of Signal Transduction, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, P. R. China.
authors.forename: Hong-Yang
authors.initials: HY
authors.lastname: Wang
authors.affiliation: 1. Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, P. R. China.
authors.forename: He
authors.initials: H
authors.lastname: Tian
authors.affiliation: 1. Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, P. R. China.
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Long
mesh_terms: D015415:Biomarkers / Q000097:blood*; D002097:C-Reactive Protein / Q000032:analysis; D003955:Diagnostic Tests, Routine / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007164:Immunosorbents / Q000378:metabolism; D008670:Metals / Q000378:metabolism*; D017430:Prostate-Specific Antigen / Q000097:blood; D012680:Sensitivity and Specificity; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D015415:Biomarkers; D007164:Immunosorbents; D008670:Metals; D000509:alpha-Fetoproteins; D002097:C-Reactive Protein; D017430:Prostate-Specific Antigen
keywords: MeLISA