title: Cardiorespiratory activity of C-terminal pentapeptide of substance P in anaesthetized rats.
abstract: Experiments were performed in anaesthetized, spontaneously breathing rats to: (1) analyse the respiratory and cardiovascular effects of C-terminal fragment of substance P (AWL2077) as referred to those exerted by the parent undecapeptide, (2) determine the involvement of lung vagal afferents to these responses. Each peptide was injected intravenously at a dose of 0.3μmol/kg into neurally intact or midcervically vagotomized rats. Administration of both compounds decreased tidal volume, minute ventilation, mean arterial blood pressure and heart rate, showing stimulatory (SP) and depressive (AWL2077) effects on the rate of breathing. Midcervical vagotomy reversed (post-SP) and precluded (post-AWL2077) respiratory rate responses and eliminated bradycardia evoked by both peptides. These findings indicate that the examined C-terminal pentapeptide was convergent with, but less potent than substance P in central depression of tidal volume and displayed divergence in the peripheral effect on respiratory timing.
authors.affiliation: Laboratory of Respiration Physiology, Mossakowski Medical Research Centre Polish Academy of Sciences, Pawińskiego 5, 02-106 Warsaw, Poland. Electronic address: pwojciechowski@imdik.pan.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Wojciechowski
authors.affiliation: Laboratory of Respiration Physiology, Mossakowski Medical Research Centre Polish Academy of Sciences, Pawińskiego 5, 02-106 Warsaw, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Szereda-Przestaszewska
authors.affiliation: Department of Neuropeptides, Mossakowski Medical Research Centre Polish Academy of Sciences, Pawińskiego 5, 02-106 Warsaw, Poland.
authors.forename: Andrzej Wojciech
authors.initials: AW
authors.lastname: Lipkowski
mesh_terms: D000704:Analysis of Variance; D000758:Anesthesia; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects*; D008297:Male; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012119:Respiration / Q000187:drug effects*; D056152:Respiratory Rate / Q000187:drug effects; D013373:Substance P / Q000737:chemistry / Q000494:pharmacology*; D013990:Tidal Volume / Q000187:drug effects; D013997:Time Factors; D014628:Vagotomy
chemical_list: D010446:Peptide Fragments; D013373:Substance P
keywords: Cardiovascular effects; Respiration; Substance P; Substance P(7–11) (AWL2077)