title: The long noncoding RNA CASC9 regulates migration and invasion in esophageal cancer.
abstract: The objective of the study was to investigate the expression and functions of CASC9 in esophageal squamous cell carcinoma (ESCC). Long noncoding RNAs (lncRNAs) upregulated in ESCC tissues were detected by RNA sequencing. Expression of CASC9 was determined from clinical samples and cell lines by qRT-PCR. The effects of CASC9 knockdown on migration and invasion were evaluated by wound healing assay, cell migration and invasion assays in vitro. We found that the lncRNA, CASC9, was markedly upregulated in ESCC tissues. Furthermore, knockdown of CASC9 significantly suppressed cell migration and invasion in vitro. Furthermore, enhanced CASC9 expression level was correlated with differentiation. The results indicated that CASC9 is significantly upregulated in ESCC tissues and may represent a new marker of poor prognosis and a potential therapeutic target for esophageal cancer intervention.
authors.affiliation: Department of Clinical Laboratory, Zhejiang Province Cancer Hospital, Hangzhou, Zhejiang, 310000, China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Department of Clinical Laboratory, Zhejiang Province Cancer Hospital, Hangzhou, Zhejiang, 310000, China.|Zhejiang Key Laboratory of Diagnosis & Treatment Technology on Thoracic Oncology (Lung and Esophagus), Hangzhou, Zhejiang, 310000, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Mao
authors.affiliation: Department of Clinical Laboratory, Zhejiang Province Cancer Hospital, Hangzhou, Zhejiang, 310000, China.
authors.forename: Yejiang
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Department of Clinical Laboratory, Zhejiang Province Cancer Hospital, Hangzhou, Zhejiang, 310000, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Zhejiang Province Cancer Hospital, Hangzhou, Zhejiang, 310000, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Su
authors.affiliation: Department of Clinical Laboratory, Zhejiang Province Cancer Hospital, Hangzhou, Zhejiang, 310000, China. zjhzxxh@163.com.|Zhejiang Key Laboratory of Diagnosis & Treatment Technology on Thoracic Oncology (Lung and Esophagus), Hangzhou, Zhejiang, 310000, China. zjhzxxh@163.com.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D016000:Cluster Analysis; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding; C000622084:long noncoding RNA CASC9, human
keywords: CASC9; esophageal cancer; invasion; long noncoding RNA; migration