title: Use of Vine-Trimming Wastes as Carrier for Amycolatopsis sp. to Produce Vanillin, Vanillyl Alcohol, and Vanillic Acid.
abstract: Raw vine-trimming wastes or the solid residues obtained after different fractionation treatments were evaluated for their suitability as Amycolatopsis sp. immobilization carriers during the bioconversion of ferulic acid into valuable phenolic compounds such as vanillin, vanillyl alcohol, and vanillic acid, the main flavor components of vanilla pods. Previously, physical-chemical characteristics of the materials were determined by quantitative acid hydrolysis and water absorption index (WAI), and microbiological characteristics by calculating the cell retention in the carrier (λ). Additionally, micrographics of carrier surface were obtained by field emission-scanning electron microscopy to study the influence of morphological changes during pretreatments in the adhesion of cells immobilized. The results point out that in spite of showing the lowest WAI and intermediate λ, raw material was the most appropriated substrate to conduct the bioconversion, achieving up to 262.9 mg/L phenolic compounds after 24 h, corresponding to 42.9 mg/L vanillin, 115.6 mg/L vanillyl alcohol, and 104.4 mg/L vanillic acid. The results showed the potential of this process to be applied for biotechnological production of vanillin from ferulic acid solutions; however, further studies must be carried out to increase vanillin yield. Additionally, the liquors obtained after treatment of vine-trimming wastes could be assayed to replace synthetic ferulic acid.
authors.affiliation: Department of Chemical Engineering, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004, Ourense, Spain.|Laboratory of Agro-food Biotechnology, CITI (University of Vigo)-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900, Ourense, Spain.|Instituto Tecnológico de Veracruz-UNIDA, Av. Miguel A. de Quevedo 2779, Col. Formando Hogar, 91860, Veracruz, VER, Mexico.
authors.forename: Juan Francisco
authors.initials: JF
authors.lastname: Castañón-Rodríguez
authors.affiliation: Department of Chemical Engineering, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004, Ourense, Spain.|Laboratory of Agro-food Biotechnology, CITI (University of Vigo)-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900, Ourense, Spain.
authors.forename: Noelia
authors.initials: N
authors.lastname: Pérez-Rodríguez
authors.affiliation: Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580, Bl 16, São Paulo, 05508-900, Brazil.
authors.forename: Ricardo Pinheiro
authors.initials: RP
authors.lastname: de Souza Oliveira
authors.affiliation: Instituto Tecnológico de Veracruz-UNIDA, Av. Miguel A. de Quevedo 2779, Col. Formando Hogar, 91860, Veracruz, VER, Mexico.
authors.forename: María Guadalupe
authors.initials: MG
authors.lastname: Aguilar-Uscanga
authors.affiliation: Department of Chemical Engineering, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004, Ourense, Spain. jmanuel@uvigo.es.|Laboratory of Agro-food Biotechnology, CITI (University of Vigo)-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900, Ourense, Spain. jmanuel@uvigo.es.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Domínguez
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D001547:Benzaldehydes / Q000378:metabolism*; D001592:Benzyl Alcohols / Q000378:metabolism*; D001709:Biotechnology; D001711:Biotransformation; D003373:Coumaric Acids / Q000378:metabolism; D014641:Vanillic Acid / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis*
chemical_list: D001547:Benzaldehydes; D001592:Benzyl Alcohols; D003373:Coumaric Acids; D014866:Waste Products; C004999:ferulic acid; C100058:vanillin; D014641:Vanillic Acid; C024078:vanillyl alcohol