title: IgE reactivity to hen egg white allergens in dogs with cutaneous adverse food reactions.
abstract: Dogs with cutaneous adverse food reactions (CAFR) often have specific IgE to food allergens. Egg white, which is majorly composed of ovomucoid, ovalbumin, ovotransferrin, and lysozyme, is a food allergen in dogs. Information of the IgE reactivity to purified egg white allergens supports accurate diagnosis and efficiency treatment in humans. However, to the best of our knowledge, there have been no studies on the IgE reactivity to purified egg white allergens in dogs. Here, we investigated the IgE reactivity to crude and purified allergens of hen egg white in dogs with CAFR. First, when we examined serum samples from 82 dogs with CAFR for specific IgE to crude egg white by ELISA, 9.8% (8/82) of the dogs with CAFR showed the IgE reactivity to crude egg white. We then used sera from the eight dogs with positive IgE reactivity to crude egg white to examine the IgE reactivity to four purified allergens, ovomucoid, ovalbumin, ovotransferrin, and lysozyme, by ELISA. We found that 75% (6/8) of the dogs showed IgE reactivity to both ovomucoid and ovalbumin, and that 37.5% (3/8) of the dogs showed IgE reactivity to ovotransferrin. None (0/8) showed IgE reactivity to lysozyme. Moreover, validating these results, the immunoblot analyses were performed using the sera of the three dogs showing the highest IgE reactivity to crude egg white. Both anti-ovomucoid and anti-ovalbumin IgE were detected in the sera of these dogs, while anti-ovotransferrin IgE was not detected. Considering these, ovomucoid and ovalbumin appears to be the major egg white allergens in dogs with CAFR.
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-5201, Japan.
authors.forename: Hidekatsu
authors.initials: H
authors.lastname: Shimakura
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-5201, Japan.
authors.forename: Jumpei
authors.initials: J
authors.lastname: Uchiyama
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-5201, Japan.
authors.forename: Taku
authors.initials: T
authors.lastname: Saito
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-5201, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Miyaji
authors.affiliation: Fujimura Animal Hospital, 5-10-26, Aomatanihigashi, Minou-shi, Osaka 652-0022, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Fujimura
authors.affiliation: Animal Allergy Clinical Laboratories, SIC-2 301, 5-4-30 Nishihashimoto, Midori-ku, Sagamihara-shi, Kanagawa 252-0131, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Masuda
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-5201, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Okamoto
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: DeBoer
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-5201, Japan. Electronic address: sakagum@azabu-u.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakaguchi
mesh_terms: D000485:Allergens / Q000302:isolation & purification; D000818:Animals; D000918:Antibody Specificity; D002645:Chickens; D003207:Conalbumin / Q000276:immunology; D003876:Dermatitis, Atopic / Q000276:immunology / Q000662:veterinary; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D021181:Egg Hypersensitivity / Q000276:immunology / Q000662:veterinary*; D004529:Egg White / Q000009:adverse effects*; D007073:Immunoglobulin E / Q000097:blood*; D009113:Muramidase / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D010059:Ovomucin / Q000276:immunology
chemical_list: D000485:Allergens; D003207:Conalbumin; D010059:Ovomucin; D007073:Immunoglobulin E; D010047:Ovalbumin; D009113:Muramidase
keywords: Cutaneous adverse food reactions; Dogs; Egg white allergens; IgE