title: [Determination of 3-hydroxyisovaleryl carnitine in plasma and urinary excretion by ultra-performance liquid chromatography-tandem mass spectrometry].
abstract: OBJECTIVE
  An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS ) method was developed for the determination of 3-hydroxyisovaleryl carnitine in plasma and urinary excretion.
  
  
  METHODS
  Plasma and urinary excretion samples were centrifugated with high speed at 3000 x g for 10 minutes, and the MCX solid phase extraction column was used to filter and purify the liquid samples. The following procedures were elution, wash-out, redissolve, detection. The chromatographic separation was achieved on a Waters Acquity UPLC BEH C18 column (2.1 mm x 50 mm, 1.7 microm), with a mobile phase composed of 0.1% formic acid in water-methanol with gradient elution. Analyte quantification was performed in the positive electrospray ionization mode and multiple monitoring. The quantification and qualification were performed using the internal standard of 3-hydroxyisovaleryl carnitine, then the evaluation for accuracy and precision were followed.
  
  
  RESULTS
  The linear range of 3-hydroxyisovaleryl carnitine were 0.1-20.0 microg/L in the plasma and 5.0-200 microg/L in urine, with both the linear correlation coefficient above 0. 99. Moreover, in three standard levels, rate of recovery in plasma and urine ranged from 78.6% to 115.6%. The RSD of six parallel determination for the 3HIA-carnitine in plasma and urine were 4.80% and 5.70% respectively. The detection and quantification limits for plasma and urine were 0.04 microg/L, 0.1 microg/L and 0.03 microg/L, 0.08 microg/L, respectively.
  
  
  CONCLUSIONS
  The sample preparation method is simple and fast, and the method can be used to analyze 3-hydroxyisovaleryl carnitine in plasma and urinary excretion efficiently and sensitively.
authors.forename: Jiuling
authors.initials: J
authors.lastname: Wei
authors.forename: Sai
authors.initials: S
authors.lastname: Fan
authors.forename: Liping
authors.initials: L
authors.lastname: Li
authors.forename: Xuesong
authors.initials: X
authors.lastname: Xiang
authors.forename: Rong
authors.initials: R
authors.lastname: Zhao
authors.forename: Zhu
authors.initials: Z
authors.lastname: Wang
mesh_terms: D002331:Carnitine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: C098116:3-hydroxyisovalerylcarnitine; D002331:Carnitine