title: Gene Delivery Method Using Photo-Responsive Poly(β-Amino Ester) as Vectors.
abstract: Nonviral vectors show great potential in delivering nucleic acids (NA) into many mammalian cells to achieve efficient gene transfection. Among these, cationic polymer is one of the most widely used nonviral gene delivery vectors, forming the polymer/NA complexes for the intracellular transportation and release of the genetic materials into the target mammalian cells. Here we describe the poly(β-amino ester) (PBAE) with the photo-responsive domain built in the polymers, as a UV-light-responsive nonviral gene delivery vector to deliver and release plasmid DNA (pDNA) into HeLa cells and achieve enhanced transfection efficiency.
authors.affiliation: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 W Green Street, Urbana, IL, 61801, USA.
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.affiliation: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 W Green Street, Urbana, IL, 61801, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 W Green Street, Urbana, IL, 61801, USA. jianjunc@illinois.edu.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Cheng
mesh_terms: D004247:DNA / Q000235:genetics*; D015316:Genetic Therapy; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles; D055668:Photochemical Processes; D010957:Plasmids / Q000235:genetics; D011108:Polymers / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
chemical_list: D011108:Polymers; C507253:poly(beta-amino ester); D049452:Green Fluorescent Proteins; D004247:DNA
keywords: Nonviral gene delivery; Photo-responsive; Poly (β-amino ester)s; Transfection efficiency