title: Green synthesis of anticancerous honeycomb PtNPs clusters: Their alteration effect on BSA and HsDNA using fluorescence probe.
abstract: The screening and characterization of cancer cells has been challenging due to sample insufficiency and extravagant. In this article, we highlighted easy green synthesis of Platinum nanoparticles (PtNPs) in the honeycomb like clusters, and their optical properties (by HRTEM, XRD, DLS, Zeta potential, EDAX, and UV-Visible techniques). PtNPs were responsive of binding mechanisms with the bovine serum albumin (BSA), herring sperm deoxyribonucleic acid (HsDNA) and cytotoxicity of human carcinomas cell. We are able to elucidate the responses of various concentrations of PtNPs for the control of MDA-MB-468 cell and binding conformation of BSA and HsDNA by using multi-spectroscopic techniques under the physiological conditions. The extent of quenching was in agreement of PtNPs-BSA binding reaction was mainly a static. The Ksv, K, the number of binding sites at different temperatures and the thermodynamic parameters between BSA and PtNPs were calculated. The positive ΔS(0) and negative ΔH(0), ΔG(0) values indicated that the binding pattern was determined by spontaneous hydrogen bond electrostatic interaction of BSA with esterage like activity. The binding properties of the PtNPs with HsDNA have been investigated by thermal denaturation, competitive DNA-binding studies with ethidium bromide (EB), Hochest-33258 and relative viscosity. The negative ΔH(0), ΔS(0) and ΔG(0) values indicated that the hydrophilic interaction were main force in spontaneity in binding mechanism of PtNPs to HsDNA. GI50 value of PtNPs demonstrated that these nanoparticles showed cytotoxicity against MDA-MB-468 human breast cancer cell line. Our results also clarified that PtNPs bind to BSA and can be effectively transported in the body and eliminated. PtNPs showed minor groove binding with HsDNA, which could be a useful guideline for further versatile approach to develop biomedical coatings with different functions of drug design.
authors.affiliation: Department of Chemistry, University of Mumbai, Santacruz (E), Mumbai 400098, India.
authors.forename: Amol V
authors.initials: AV
authors.lastname: Pansare
authors.affiliation: Department of Chemistry, University of Mumbai, Santacruz (E), Mumbai 400098, India.
authors.forename: Dnyaneshwar K
authors.initials: DK
authors.lastname: Kulal
authors.affiliation: Department of Chemistry, University of Mumbai, Santacruz (E), Mumbai 400098, India.
authors.forename: Amol A
authors.initials: AA
authors.lastname: Shedge
authors.affiliation: Department of Chemistry, University of Mumbai, Santacruz (E), Mumbai 400098, India. Electronic address: vishwanathrpatil03@gmail.com.
authors.forename: Vishwanath R
authors.initials: VR
authors.lastname: Patil
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001665:Binding Sites; D002417:Cattle; D045744:Cell Line, Tumor; D060326:Chemistry Techniques, Synthetic; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004950:Esterases / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D055772:Green Chemistry Technology; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles; D036103:Nanotechnology; D009691:Nucleic Acid Denaturation; D010984:Platinum / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013094:Spermatozoa; D044366:Transition Temperature
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine; D010984:Platinum; D004247:DNA; D004950:Esterases
keywords: BSA; EB and Hochest-33,258; HsDNA; MDA-MB-468; PtNPs