title: Detection and quantification of ATP in human blood serum.
abstract: Two fluorometric sensors based on the tri-serine tri-lactone scaffold and thiourea or sulfonamide moieties serving as hydrogen bond donors allowing for anion binding are described. The sensor utilizing thiourea as a recognition moiety shows fluorescence enhancement while the sensor with sulfonamide shows quenching upon addition of phosphates. Sensor arrays composed of two sensors are able to discriminate structurally similar organic phosphates in the presence of interferents in human blood serum. The quantitative analysis of ATP in human blood serum shows high accuracy (the root mean square error of prediction, 1.65%) without requiring any sample pretreatment.
authors.affiliation: Department of Chemistry and Center for Photochemical Sciences, Bowling Green State University, Bowling Green, Ohio 43403, U.S.A. pavel@bgsu.edu.
authors.forename: Ali
authors.initials: A
authors.lastname: Akdeniz
authors.forename: Mehmet Gokhan
authors.initials: MG
authors.lastname: Caglayan
authors.forename: Irina
authors.initials: I
authors.lastname: Polivina
authors.forename: Pavel
authors.initials: P
authors.lastname: Anzenbacher
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006860:Hydrogen Bonding; D007783:Lactones / Q000737:chemistry; D015394:Molecular Structure; D012694:Serine / Q000737:chemistry; D013449:Sulfonamides / Q000737:chemistry; D013890:Thiourea / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D007783:Lactones; D013449:Sulfonamides; D012694:Serine; D000255:Adenosine Triphosphate; D013890:Thiourea