title: Effects of ultrasonic processing on degradation of salvianolic acid B in aqueous solution.
abstract: To evaluate the stability of salvianolic acid B (Sal B) under ultrasound-assisted extraction in the pharmaceutical industry, degradation of Sal B under ultrasonic irradiation was investigated as the function of buffer concentration, pH, and temperature. With regard to Sal-B concentration, a first-order degradation process was determined, with 10% change in assay from its initial concentration as t90=4.81h, under maximum stability acidic conditions (pH 2.0) and at 25°C. The logkpH-pH profile described by specific acid-base catalysis and water molecules supported the experimental results. Liquid chromatography-mass spectrometry (LC-MS) analyses revealed 7 major degradation products whose structures were characterized by electrospray ionization/mass spectrometry. A primary degradation pathway involved cleavage of the ester bond and ring-opening of benzofuran in Sal B was proposed. The complete degradation pathway of Sal B was also proposed. Results showed that ultrasonic irradiation leads to degradation of Sal B in aqueous solution.
authors.affiliation: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China. Electronic address: yongxueguo@163.com.
authors.forename: Y X
authors.initials: YX
authors.lastname: Guo
authors.affiliation: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China.
authors.forename: L
authors.initials: L
authors.lastname: Lu
authors.affiliation: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China.
authors.forename: E H
authors.initials: EH
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China.
authors.forename: C Z
authors.initials: CZ
authors.lastname: Shi
mesh_terms: D001572:Benzofurans / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D012996:Solutions / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013696:Temperature; D014465:Ultrasonics / Q000379:methods; D014867:Water / Q000737:chemistry*; D053630:Depsides
chemical_list: D001572:Benzofurans; D012996:Solutions; D014867:Water; C076944:salvianolic acid B; D053630:Depsides; C105430:benzofuran
keywords: Degradation; Kinetics; LC–MS; Salvianolic acid B; Ultrasonic irradiation