title: Rapid and efficient gene delivery into the adult mouse brain via focal electroporation.
abstract: In vivo gene delivery is required for studying the cellular and molecular mechanisms of various biological events. Virus-mediated gene transfer or generation of transgenic animals is widely used; however, these methods are time-consuming and expensive. Here we show an improved electroporation technique for acute gene delivery into the adult mouse brain. Using a syringe-based microelectrode, local DNA injection and the application of electric current can be performed simultaneously; this allows rapid and efficient gene transduction of adult non-neuronal cells. Combining this technique with various expression vectors that carry specific promoters resulted in targeted gene expression in astrocytic cells. Our results constitute a powerful strategy for the genetic manipulation of adult brains in a spatio-temporally controlled manner.
authors.affiliation: Developmental Neurobiology, Kyoto Prefectural University of Medicine, INAMORI Memorial Building, 1-5 Shimogamo-hangicho, Sakyoku, Kyoto, 606-0823, Japan.|Japan Science and Technology Agency, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nomura
authors.affiliation: Developmental Neurobiology, Kyoto Prefectural University of Medicine, INAMORI Memorial Building, 1-5 Shimogamo-hangicho, Sakyoku, Kyoto, 606-0823, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nishimura
authors.affiliation: Developmental Neurobiology, Kyoto Prefectural University of Medicine, INAMORI Memorial Building, 1-5 Shimogamo-hangicho, Sakyoku, Kyoto, 606-0823, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Gotoh
authors.affiliation: Developmental Neurobiology, Kyoto Prefectural University of Medicine, INAMORI Memorial Building, 1-5 Shimogamo-hangicho, Sakyoku, Kyoto, 606-0823, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Ono
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D008839:Microelectrodes; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D015203:Reproducibility of Results; D014162:Transfection / Q000379:methods
chemical_list: D049452:Green Fluorescent Proteins; D004247:DNA