title: SnoN suppresses TGF-β-induced epithelial-mesenchymal transition and invasion of bladder cancer in a TIF1γ-dependent manner.
abstract: The transcriptional regulator SnoN (also known as SKI-like proto-oncogene, SKIL), a member of the Ski family, has been reported to influence epithelial-mesenchymal transition (EMT) in response to TGF-β. In the present study, we investigated the role of SnoN in bladder cancer (BC). Differential expression of SnoN was not detected in BC tissues compared with that noted in adjacent non-cancerous tissues. SnoN was upregulated in response to TGF-β treatment, but had no effect on the TGF-β pathway, which may be explained by the low level of SnoN SUMOylation. TIF1γ, which catalyzes the SUMOylation of SnoN, was downregulated in BC tissues. Overexpression of TIF1γ restored the ability of SnoN to suppress the TGF-β pathway. Furthermore, TGF-β-induced EMT and invasion of BC cells were suppressed by TIF1γ in the presence of SnoN. Collectirely, our data suggest that SnoN suppresses TGF-β‑induced EMT and invasion of BC cells in a TIF1γ‑dependent manner and may serve as a novel therapeutic option for the treatment of BC.
authors.affiliation: Department of Urology, Qilu Hospital of Shandong University, Qingdao, Shandong 266035, P.R. China.
authors.forename: Xinbao
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
authors.forename: Chuanshen
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Urology, Qilu Hospital of Shandong University, Qingdao, Shandong 266035, P.R. China.
authors.forename: Huiyang
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Urology, Qilu Hospital of Shandong University, Qingdao, Shandong 266035, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Urology, Qilu Hospital of Shandong University, Qingdao, Shandong 266035, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of Urology, Qilu Hospital of Shandong University, Qingdao, Shandong 266035, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
mesh_terms: D015153:Blotting, Western; D002295:Carcinoma, Transitional Cell / Q000378:metabolism / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D047468:Immunoprecipitation; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000473:pathology; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; C060615:SKIL protein, human; C511956:TRIM33 protein, human; D014157:Transcription Factors; D016212:Transforming Growth Factor beta