title: Investigation of serotype distribution and resistance genes profile in group B Streptococcus isolated from pregnant women: a Chinese multicenter cohort study.
abstract: We surveyed the group B Streptococcus (GBS) strains isolated from four teaching hospitals during 1-year period to investigate the current serotypes and antimicrobial resistance status of these strains. A total of 231 non-duplicate colonizing GBS isolates were collected from pregnant women. Antimicrobial susceptibility of these isolates was tested by the disk diffusion method. Serotype was performed by a multiplex polymerase chain reaction (PCR) method. Analysis of the resistance mechanisms was performed by PCR amplification and DNA sequencing. Seven serotypes (Ia, Ib, II, III, V, VI, and VIII) were identified, and the prevalence ranged from 0.9 to 35.9%. All isolates were susceptible to the penicillin, ceftriaxone, and vancomycin. The resistance of all the isolates to erythromycin, clindamycin, and levofloxacin was 61.5, 51.9, and 35.5%, respectively. The erythromycin resistance was mainly associated with the genes ermB and ermB-mef(A/E) (69.8%). The most predominant phenotype was cMLSB (77.5%). Five gene panels, including gyrA, parC, parE, gyrA-parC, and gyrA-parC-parE, were detected. The most predominant genotype was gyrA-parC-parE triple mutation (69.5%). The S81L in gyrA gene, S79Y mutation in parC gene, and H225Y mutation in parE gene were discovered. The isolates with serotype III, V, and Ia were the most important clone concerning the prevalence and resistance.
authors.affiliation: Department of Transfusion Medicine, Shanghai Huashan Hospital, Fudan University, Shanghai, China.|Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University, Shanghai, China.
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Gynaecology and Obstetrics, Shanghai Pudong Hospital, Fudan University, Shanghai, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Clinical Laboratory, Shanghai International Peace Maternity & Child Health Hospital, Jiaotong University, Shanghai, China.
authors.forename: Tingyan
authors.initials: T
authors.lastname: Lu
authors.affiliation: Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University, Shanghai, China.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Clinical Laboratory, Shanghai Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Shanghai First Maternity & Infant Hospital, Tongji University, Shanghai, China.
authors.forename: Xianhua
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University, Shanghai, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Transfusion Medicine, Shanghai Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xia
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002681:China / Q000453:epidemiology; D015331:Cohort Studies; D052978:Disk Diffusion Antimicrobial Tests; D024881:Drug Resistance, Bacterial; D005260:Female; D005838:Genotype; D006784:Hospitals, Teaching; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000453:epidemiology / Q000382:microbiology*; D015995:Prevalence; D017422:Sequence Analysis, DNA; D065288:Serogroup; D013290:Streptococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013292:Streptococcus agalactiae / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Group B Streptococcus; antimicrobial susceptibility; phenotype; serotype