title: Regulation of SATB1 during thymocyte development by TCR signaling.
abstract: T lymphocyte development and differentiation is a multi-step process that begins in the thymus and completed in the periphery. Sequential development of thymocytes is dependent on T cell receptor (TCR) signaling and an array of transcription factors. In this study we show that special AT-rich binding protein 1 (SATB1), a T lineage-enriched chromatin organizer and regulator, is induced in response to TCR signaling during early thymocyte development. SATB1 expression profile coincides with T lineage commitment and upregulation of SATB1 correlates with positive selection of thymocytes. CD4 thymocytes exhibit a characteristic bimodal expression pattern that corresponds to immature and mature CD4 thymocytes. We also demonstrate that GATA3, the key transcriptional regulator of αβ T cells positively regulates SATB1 expression in thymocytes suggesting an important role for SATB1 during T cell development.
authors.affiliation: Indian Institute of Science Education and Research, Pune 411008, India.
authors.forename: Kamalvishnu P
authors.initials: KP
authors.lastname: Gottimukkala
authors.affiliation: Indian Institute of Science Education and Research, Pune 411008, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Jangid
authors.affiliation: Indian Institute of Science Education and Research, Pune 411008, India.
authors.forename: Indumathi
authors.initials: I
authors.lastname: Patta
authors.affiliation: National Institute on Aging, NIH and School of Medicine Immunology Graduate Program, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: Dil Afroz
authors.initials: DA
authors.lastname: Sultana
authors.affiliation: National Institute on Aging, NIH and School of Medicine Immunology Graduate Program, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: Archna
authors.initials: A
authors.lastname: Sharma
authors.affiliation: National Institute on Aging, NIH and School of Medicine Immunology Graduate Program, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Misra-Sen
authors.affiliation: Indian Institute of Science Education and Research, Pune 411008, India; National Centre for Cell Science, Ganeshkhind, Pune 411007, India. Electronic address: sanjeev@iiserpune.ac.in.
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Galande
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis* / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology*; D047369:Chromatin Immunoprecipitation; D005434:Flow Cytometry; D050990:GATA3 Transcription Factor / Q000096:biosynthesis / Q000276:immunology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D015151:Immunoblotting; D051379:Mice; D008810:Mice, Inbred C57BL; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology; D060168:Thymocytes / Q000166:cytology*; D059467:Transcriptome
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D011948:Receptors, Antigen, T-Cell; C486732:Stab1 protein, mouse
keywords: GATA-3; SATB1; Signaling; T cell receptor