title: Optimizing multi-dimensional high throughput screening using zebrafish.
abstract: The use of zebrafish for high throughput screening (HTS) for chemical bioactivity assessments is becoming routine in the fields of drug discovery and toxicology. Here we report current recommendations from our experiences in zebrafish HTS. We compared the effects of different high throughput chemical delivery methods on nominal water concentration, chemical sorption to multi-well polystyrene plates, transcription responses, and resulting whole animal responses. We demonstrate that digital dispensing consistently yields higher data quality and reproducibility compared to standard plastic tip-based liquid handling. Additionally, we illustrate the challenges in using this sensitive model for chemical assessment when test chemicals have trace impurities. Adaptation of these better practices for zebrafish HTS should increase reproducibility across laboratories.
authors.affiliation: Department of Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, and the Environmental Health Sciences Center at Oregon State University, Corvallis, OR, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Truong
authors.affiliation: Department of Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, and the Environmental Health Sciences Center at Oregon State University, Corvallis, OR, USA.
authors.forename: Sean M
authors.initials: SM
authors.lastname: Bugel
authors.affiliation: Department of Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, and the Environmental Health Sciences Center at Oregon State University, Corvallis, OR, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Chlebowski
authors.affiliation: Department of Biology, Baylor University, Waco, TX, USA.
authors.forename: Crystal Y
authors.initials: CY
authors.lastname: Usenko
authors.affiliation: Department of Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, and the Environmental Health Sciences Center at Oregon State University, Corvallis, OR, USA.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Simonich
authors.affiliation: Department of Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, and the Environmental Health Sciences Center at Oregon State University, Corvallis, OR, USA.
authors.forename: Staci L Massey
authors.initials: SL
authors.lastname: Simonich
authors.affiliation: Department of Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, and the Environmental Health Sciences Center at Oregon State University, Corvallis, OR, USA. Electronic address: Robert.Tanguay@oregonstate.edu.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Tanguay
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian; D004958:Estradiol / Q000494:pharmacology; D004997:Ethinyl Estradiol / Q000494:pharmacology; D057166:High-Throughput Screening Assays; D013893:Thiram / Q000494:pharmacology; D015027:Zebrafish
chemical_list: D013893:Thiram; D004997:Ethinyl Estradiol; D004958:Estradiol
keywords: Developmental toxicity; High throughput screening; Zebrafish