title: Morphological study of dynamic culture of thermosensitive collagen hydrogel in constructing tissue engineering complex.
abstract: ABSTACT The purpose of this study is to research the morphologies and functional characteristics of the cell-scaffold complex in vitro constructed under dynamic culture conditions. BMSCs were isolated from the long bones of Fischer344 rats, and performed in vitro amplification to the third generation as seed cells, together with thermosensitive collagen hydrogel (TCH) as cell adhesion matrix, and poly-L-lactic acid (PLLA) as scaffold, to construct cell-scaffold complex. The cell-scaffold complexes in the experiment group and the control group were then performed dynamic culture and static culture. After 7 d of in vitro culture, the complexes in the 2 groups were performed gross observation and SEM; meanwhile, the total DNA content in the complex was detected on D0,1,3, and 7 of culture. After cultured using these 2 ways, collagen could both wrap the PLLA scaffold, forming dense film-like structures on the PLLA surface. The total DNA contents in the cell-scaffold complex of the experiment group on D1,3, and 7 were significantly higher than the control group (P < 0.05). Compared with D0, the total DNA contents on D1,3, and 7 in both groups were gradually increased, but only the total DNA contents on D7 showed statistically significant difference than D0 (P < 0.05). TCH -PLLA fiber joint-constructed complex extracellular matrix had good biocompatibility, and dynamic culture could promote the distribution of BMSCs on the surface and inside the structure, thus promoting cell proliferation, so it could be used for the in vitro construction of tissue engineering complex.
authors.affiliation: a Department of Orthopedics , The Second Hospital of Jilin University , Changchun , China.
authors.forename: Lanfeng
authors.initials: L
authors.lastname: Huang
authors.affiliation: a Department of Orthopedics , The Second Hospital of Jilin University , Changchun , China.
authors.forename: Feixiang
authors.initials: F
authors.lastname: Xu
authors.affiliation: a Department of Orthopedics , The Second Hospital of Jilin University , Changchun , China.
authors.forename: Bin
authors.initials: B
authors.lastname: Guo
authors.affiliation: a Department of Orthopedics , The Second Hospital of Jilin University , Changchun , China.
authors.forename: Jianchao
authors.initials: J
authors.lastname: Ma
authors.affiliation: b Department of Ophthalmology , The Second Hospital of Jilin University , Changchun , China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry; D006358:Hot Temperature; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D051381:Rats; D011916:Rats, Inbred F344; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D011091:Polyesters; C033616:poly(lactide); D003094:Collagen
keywords: dynamic culture; extracellular matrix; scaffold; seed cells; tissue engineering