title: A single Mediterranean meal does not impair postprandial flow-mediated dilatation in healthy men with subclinical metabolic dysregulations.
abstract: Cardiovascular risk factors are known to exacerbate high-saturated fatty acid meal (HSFAM)-induced endothelial dysfunction, but the influence of subclinical metabolic dysregulations and the acute impact of a single mixed Mediterranean-type meal (MMM) remains unknown. Thus, this study has the objective to evaluate the metabolic and vascular effect of such meals in healthy subjects with or without subclinical fasting metabolic dysregulations. Twenty-eight healthy males without overt cardiovascular risk factors randomly ingested 1 of 2 isocaloric meals on separate days. Plasma metabolic markers, fatty acid (FA) profile, and endothelial function (flow-mediated dilatation; FMD) were assessed at baseline and 2 and 4 h after meal ingestion. Unsupervised hierarchical clustering identified 2 subgroups of participants (n = 11 and 17) differing by their baseline metabolic profiles. The MMM did not significantly alter postprandial endothelial function in all subjects, irrespective of baseline metabolic parameters. In contrast, the HSFAM induced postprandial endothelial dysfunction (Δ%FMDabsolute = -5.28 ± 2.54, p < 0.01 vs. MMM) in a subgroup of individuals with significantly greater body mass index, fasting insulinemia, and lipid parameters (n = 11). Finally, the postprandial plasma FA profiles were differentially enriched by the HSFAM and MMM, notably with saturated FAs and omega-3 polyunsaturated FAs, respectively. Collectively, our results highlight the detrimental impact of a single HSFAM on endothelial function in healthy individuals displaying subclinical fasting metabolic dysregulations. Such individuals could benefit from MMM, demonstrated herein to be without any acute detriment to endothelial function.
authors.affiliation: a Cardiovascular Prevention and Rehabilitation Centre, Montreal Heart Institute, 5055 St-Zotique Street East, Montreal, QC H1T 1N6, Canada.|b Montreal Heart Institute Research Center, 5000 Belanger Street, Montreal, QC H1T 1C8, Canada.|c Department of Nutrition, Faculty of Medicine, Université de Montréal, 2405, Côte-Sainte-Catherine Road, Montréal, QC H3T 1A8, Canada.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lacroix
authors.affiliation: a Cardiovascular Prevention and Rehabilitation Centre, Montreal Heart Institute, 5055 St-Zotique Street East, Montreal, QC H1T 1N6, Canada.|b Montreal Heart Institute Research Center, 5000 Belanger Street, Montreal, QC H1T 1C8, Canada.|c Department of Nutrition, Faculty of Medicine, Université de Montréal, 2405, Côte-Sainte-Catherine Road, Montréal, QC H3T 1A8, Canada.
authors.forename: Christine
authors.initials: C
authors.lastname: Des Rosiers
authors.affiliation: a Cardiovascular Prevention and Rehabilitation Centre, Montreal Heart Institute, 5055 St-Zotique Street East, Montreal, QC H1T 1N6, Canada.|b Montreal Heart Institute Research Center, 5000 Belanger Street, Montreal, QC H1T 1C8, Canada.|d Department of Medicine, Université de Montréal, 2900, Édouard-Montpetit Blvd., Montréal, QC H3T 1J4, Canada.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Gayda
authors.affiliation: e Montreal Heart Institute Coordinating Center, 5000 Belanger Street, Montreal, QC H1T 1C8, Canada.
authors.forename: Anna
authors.initials: A
authors.lastname: Nozza
authors.affiliation: b Montreal Heart Institute Research Center, 5000 Belanger Street, Montreal, QC H1T 1C8, Canada.|f Department of Surgery, Université de Montréal, 2900, Édouard-Montpetit Blvd., Montréal, QC H3T 1J4, Canada.
authors.forename: Éric
authors.initials: É
authors.lastname: Thorin
authors.affiliation: b Montreal Heart Institute Research Center, 5000 Belanger Street, Montreal, QC H1T 1C8, Canada.|d Department of Medicine, Université de Montréal, 2900, Édouard-Montpetit Blvd., Montréal, QC H3T 1J4, Canada.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Tardif
authors.affiliation: a Cardiovascular Prevention and Rehabilitation Centre, Montreal Heart Institute, 5055 St-Zotique Street East, Montreal, QC H1T 1N6, Canada.|b Montreal Heart Institute Research Center, 5000 Belanger Street, Montreal, QC H1T 1C8, Canada.|c Department of Nutrition, Faculty of Medicine, Université de Montréal, 2405, Côte-Sainte-Catherine Road, Montréal, QC H3T 1A8, Canada.|d Department of Medicine, Université de Montréal, 2900, Édouard-Montpetit Blvd., Montréal, QC H3T 1J4, Canada.
authors.forename: Anil
authors.initials: A
authors.lastname: Nigam
mesh_terms: D000293:Adolescent; D000328:Adult; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D018592:Cross-Over Studies; D038441:Diet, Mediterranean; D004106:Dilatation; D004730:Endothelium, Vascular / Q000502:physiology*; D005215:Fasting; D005227:Fatty Acids / Q000097:blood; D015525:Fatty Acids, Omega-3 / Q000097:blood; D064368:Healthy Volunteers; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D062407:Meals; D008875:Middle Aged; D019518:Postprandial Period; D014280:Triglycerides / Q000097:blood; D055815:Young Adult
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D007328:Insulin; D014280:Triglycerides
keywords: cardiovascular disease risk factors; composition d’acides gras plasmatiques; endothelial function; facteurs de risque de maladie cardiovasculaire; fonction endothéliale; inflammation; oxidative stress; plasma fatty acid composition; stress oxydatif