title: Large-scale image-based screening and profiling of cellular phenotypes.
abstract: Cellular phenotypes are observable characteristics of cells resulting from the interactions of intrinsic and extrinsic chemical or biochemical factors. Image-based phenotypic screens under large numbers of basal or perturbed conditions can be used to study the influences of these factors on cellular phenotypes. Hundreds to thousands of phenotypic descriptors can also be quantified from the images of cells under each of these experimental conditions. Therefore, huge amounts of data can be generated, and the analysis of these data has become a major bottleneck in large-scale phenotypic screens. Here, we review current experimental and computational methods for large-scale image-based phenotypic screens. Our focus is on phenotypic profiling, a computational procedure for constructing quantitative and compact representations of cellular phenotypes based on the images collected in these screens. © 2016 International Society for Advancement of Cytometry.
authors.affiliation: Bioinformatics Institute, Agency for Science, Technology and Research, Singapore, 138671, Singapore.
authors.forename: Nicola
authors.initials: N
authors.lastname: Bougen-Zhukov
authors.affiliation: Bioinformatics Institute, Agency for Science, Technology and Research, Singapore, 138671, Singapore.
authors.forename: Sheng Yang
authors.initials: SY
authors.lastname: Loh
authors.affiliation: Bioinformatics Institute, Agency for Science, Technology and Research, Singapore, 138671, Singapore.
authors.forename: Hwee Kuan
authors.initials: HK
authors.lastname: Lee
authors.affiliation: Bioinformatics Institute, Agency for Science, Technology and Research, Singapore, 138671, Singapore.|Department of Pharmacology, School of Medicine, National University of Singapore, Singapore, 117600, Singapore.
authors.forename: Lit-Hsin
authors.initials: LH
authors.lastname: Loo
mesh_terms: D058948:Cell Tracking; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D010641:Phenotype
keywords: automated image analysis; cellular phenotypes; high-content screening; high-throughput microscopy; imaging-based phenotypic screens; phenotypic profiling