title: Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR.
abstract: Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the market. In addition, weeds reduce alfalfa yield by about 50 %. Although weeds are the limiting factor for alfalfa production, little progress has been made in the incorporation of herbicide-tolerant traits into commercial alfalfa. This is partially due to the high times and costs needed for the production of vast numbers of transgenic alfalfa events as an empirical approach to bypass the random transgenic silencing and for the identification of an event with optimal transgene expression. In this focus article, we report the complete sequence of pPZP200BAR and the extremely high efficiency of this binary vector in alfalfa transformation, opening the way for rapid and inexpensive production of transgenic events for alfalfa improvement public programs.
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.|Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los Reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina.
authors.forename: Cintia
authors.initials: C
authors.lastname: Jozefkowicz
authors.affiliation: Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los Reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina.
authors.forename: Emilia
authors.initials: E
authors.lastname: Bottero
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.|Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los Reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Pascuan
authors.affiliation: Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los Reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina.
authors.forename: Elba
authors.initials: E
authors.lastname: Pagano
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.|Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los Reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina.
authors.forename: Nicolás Daniel
authors.initials: ND
authors.lastname: Ayub
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina. soto.gabrielacinthia@inta.gob.ar.|Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los Reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina. soto.gabrielacinthia@inta.gob.ar.
authors.forename: Gabriela
authors.identifier: http://orcid.org/0000-0002-3492-1268
authors.initials: G
authors.lastname: Soto
mesh_terms: D003365:Costs and Cost Analysis; D015723:Gene Library; D005821:Genetic Techniques / Q000191:economics*; D005822:Genetic Vectors / Q000378:metabolism*; D000455:Medicago sativa / Q000235:genetics*; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014170:Transformation, Genetic
keywords: Alfalfa; Binary vector; Complete sequence; Transformation; pPZP200BAR