title: Insecticidal Activity and Histopathological Effects of Vip3Aa Protein fromBacillus thuringiensisonSpodoptera litura.
abstract: Vegetative insecticidal proteins (Vips) are insecticidal proteins synthesized byBacillus thuringiensisduring the vegetative stage of growth. In this study, Vip3Aa protein, obtained by in vitro expression of thevip3Aagene fromB. thuringiensisWB5, displayed high insecticidal activity againstSpodoptera lituraaside fromSpodoptera exiguaandHelicoverpa armigera. Bioassay results showed that the toxicity of Vip3Aa protein againstS. lituralarvae statistically decreased along with the increase of the age of the larvae, with LC50= 2.609 ng/cm2for neonatal larvae, LC50= 28.778 ng/cm2for first instar larvae, LC50= 70.460 ng/cm2for second instar larvae, and LC50= 200.627 ng/cm2for third instar larvae. The accumulative mortality of 100% larvae appeared at 72 h for all instars ofS. lituralarvae, when feeding respectively with 83.22, 213.04, 341.40, and 613.20 ng/cm2of Vip3Aa toxin to the neonatal and first to third instar larvae. The histopathological effects of Vip3Aa toxin on the midgut epithelial cells ofS. lituralarvae was also investigated. The TEM observations showed wide damage of the epithelial cell in the midgut ofS. lituralarvae fed with Vip3Aa toxin.
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, P.R. China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Song
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, P.R. China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, P.R. China.|China National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, P.R. China.
authors.forename: Ensi
authors.initials: E
authors.lastname: Shao
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, P.R. China.|Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, P.R. China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Guan
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, P.R. China.|Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, P.R. China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007306:Insecticides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects*; D010572:Pest Control, Biological; D018411:Spodoptera / Q000187:drug effects*
chemical_list: D001426:Bacterial Proteins; D007306:Insecticides; C447139:Vip3A protein, Bacillus thuringiensis
keywords: Spodoptera litura; Vip3Aa protein; histopathological effects; toxicity