title: Normal human mitral valve proteome: A preliminary investigation by gel-based and gel-free proteomic approaches.
abstract: The mitral valve is a highly complex structure which regulates blood flow from the left atrium to the left ventricle (LV) avoiding a significant forward gradient during diastole or regurgitation during systole. The integrity of the mitral valve is also essential for the maintenance of normal LV size, geometry, and function. Significant advances in the comprehension of the biological, functional, and mechanical behavior of the mitral valve have recently been made. However, current knowledge of protein components in the normal human mitral valve is still limited and complicated by the low cellularity of this tissue and the presence of high abundant proteins from the extracellular matrix. We employed here an integrated proteomic approach to analyse the protein composition of the normal human mitral valve and reported confident identification of 422 proteins, some of which have not been previously described in this tissue. In particular, we described the ability of pre-MS separation technique based on liquid-phase IEF and SDS-PAGE to identify the largest number of proteins. We also demonstrated that some of these proteins, e.g. αB-Crystallin, septin-11, four-and-a-half LIM domains protein 1, and dermatopontin, are synthesised by interstitial cells isolated from human mitral valves. These initial results provide a valuable basis for future studies aimed at analysing in depth the mitral valve protein composition and at investigating potential pathogenetic molecular mechanisms. Data are available via ProteomeXchange with identifier PXD004397.
authors.affiliation: Centro Cardiologico Monzino, IRCCS, Milan, Italy.
authors.forename: Maura
authors.initials: M
authors.lastname: Brioschi
authors.affiliation: Centro Cardiologico Monzino, IRCCS, Milan, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Baetta
authors.affiliation: Centro Cardiologico Monzino, IRCCS, Milan, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Ghilardi
authors.affiliation: Centro Cardiologico Monzino, IRCCS, Milan, Italy.
authors.forename: Erica
authors.initials: E
authors.lastname: Gianazza
authors.affiliation: Centro Cardiologico Monzino, IRCCS, Milan, Italy.|Banca Tessuti Cardiovascolari Regione Lombardia, Centro Cardiologico Monzino IRCCS Milan, Milan, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Guarino
authors.affiliation: Dipartimento di Chirurgia cardiaca, IRCCS Policlinico San Donato, Milan, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Parolari
authors.affiliation: Centro Cardiologico Monzino, IRCCS, Milan, Italy.|Università degli Studi di Milano, Dipartimento di Scienze Cliniche e di Comunità, Sezione Cardiovascolare, Milan, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Polvani
authors.affiliation: Centro Cardiologico Monzino, IRCCS, Milan, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Tremoli
authors.affiliation: Centro Cardiologico Monzino, IRCCS, Milan, Italy. cristina.banfi@ccfm.it.
authors.forename: Cristina
authors.initials: C
authors.lastname: Banfi
mesh_terms: D000328:Adult; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D008943:Mitral Valve / Q000737:chemistry*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
chemical_list: D020543:Proteome
keywords: 2-dimensional electrophoresis (2-DE); Human normal mitral valve; LC/MSE; Liquid-phase IEF; Valvular interstitial cells