title: Validation studies of the latex agglutination test for the detection of Trichinella larvae in meat products.
abstract: Human trichinellosis is a foodborne disease caused by ingestion of meat infected with Trichinella muscle larvae. To control Trichinella spp. infection in the European Union, all slaughtered pigs from holdings that are not officially recognized as applying controlled housing conditions and other animals susceptible to Trichinella infection and intended for human consumption should be examined by one of the approved digestion methods described in Regulation (EU) No. 2015/1375. In the past, Trichinella outbreaks due to the consumption of cured wild boar or pork products have been described in several European countries, making the identification of the larvae from these products relevant for Trichinella control. Therefore, this study aimed to validate the newly approved latex agglutination test (Trichin-L) for routine testing of cured meat products. The test was validated based on the OIE Guidelines using pork products spiked with Trichinella larvae. The sensitivity of the method varied greatly depending on the investigated meat product and was usually lower than for the gold standard, the magnetic stirrer method. The detection rate reached 80% for three larvae and 60% for one larva in cured pork sausages. A detection rate of 100% for three larvae and 50% for one larva was found in bacon. For frozen samples (-20°C) the Trichin-L kit is similarly sensitive as for cured samples. Further, to determine the performance of the test under field conditions, pork products from regions with known high Trichinella prevalences confiscated by customs authorities at two German international airports were analyzed. Problems associated with the Trichin-L test were incomplete digestion due to fatty ingredients, spices and very dry meat products, resulting in data which could not be evaluated. Therefore, the test is currently not suitable for the detection of Trichinella larvae in cured meat products and needs further adaptation steps to increase both usability and sensitivity.
authors.affiliation: Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany. Electronic address: jennifer.gayda@bfr.bund.de.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Gayda
authors.affiliation: Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Reckinger
authors.affiliation: Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany.
authors.forename: Nora
authors.initials: N
authors.lastname: Thaben
authors.affiliation: Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: Nöckler
authors.affiliation: Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Mayer-Scholl
mesh_terms: D000818:Animals; D005513:Food Inspection; D016266:Food Parasitology / Q000379:methods* / Q000592:standards; D007841:Latex Fixation Tests / Q000379:methods* / Q000592:standards; D008461:Meat Products / Q000469:parasitology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014234:Trichinella / Q000302:isolation & purification*
keywords: Cured meat products; Detection; Latex agglutination test; Sausage; Trichinella spiralis; Validation