title: Diagnostic significance of suppressor of cytokine signalling 3 (SOCS3) methylation and its correlation with IDH1 mutation in Chinese glioma patients.
abstract: BACKGROUND
  Epigenetic alterations in suppressor of cytokine signalling 3 (SOCS3) have been suggested as a potential biomarker in glioma.
  
  
  OBJECTIVE
  To investigate whether SOCS3 methylation could act as a biomarker for glioma grading and prognosis.
  
  
  METHODS
  Glioma samples were evaluated by pyrosequencing and quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  SOCS3 promoter methylation was more frequent in the WHO III and (anaplastic) oligoastrocytomas group. SOCS3 methylation status was significantly inversely correlated with mRNA expression level. Mutant IDH1 caused marked increase in SOCS3 methylation.
  
  
  CONCLUSIONS
  SOCS3 methylation is a potential biomarker for grading and prognosis in human glioma.
authors.affiliation: a The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University , Xi'an , PR China.
authors.forename: Weili
authors.initials: W
authors.lastname: Jiao
authors.affiliation: a The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University , Xi'an , PR China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Xun
authors.affiliation: a The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University , Xi'an , PR China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Liu
authors.affiliation: a The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University , Xi'an , PR China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Yang
authors.affiliation: a The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University , Xi'an , PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: a The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University , Xi'an , PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: a The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University , Xi'an , PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: a The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University , Xi'an , PR China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Wang
authors.affiliation: a The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University , Xi'an , PR China.
authors.forename: Penggao
authors.initials: P
authors.lastname: Dai
keywords: Biomarker; IDH1 mutation; SOCS3 methylation; glioma; pyrosequencing