title: Preparation, characterization and biodistribution in quails of99mTc-folic acid/chitosan nanostructure.
abstract: Chitosan (CS) of low molecular weight is prepared using γ-irradiation method in presence of H2O2as oxidizing agent. The chemical treatment of folic acid (FA) with low molecular weight CS is carried out to prepare FACS complex based on the reaction between NH2group of CS and γ-COOH group of FA. The structure and properties of FACS complex was characterized by FT-IR,1H NMR, UV, SEM, TEM, DLS and XRD analyses. TEM and DLS results showed that FACS complex has nanostructure and the mean size of particles was unimodal with average diameters in the range of 165-252nm. Radiolabeling of FACS complex (99mTc-FACS) was done with Technetium-99m (99mTc). The optimum conditions of labeling were investigated. The labeling yield was 85% at pH=6 and 30min reaction time. The effect of time on the stability of99mTc-FACS complex was studied and the results revealed that it is stable up to 6h after labeling. Biodistribution studies of99mTc-FACS complex in Quail showed that it distributed to different organs mainly in blood, liver and kidney. The results revealed that the uptake of99mTc-FACS in the sexual organs (ovary and ovarian) of female Quail was higher than that in the sexual organs (testes) of male Quail, so99mTc-FACS could be used to differentiate between them. It also showed that FACS is consumed and more essential in female than that in male.
authors.affiliation: Nuclear Research Center, Atomic Energy Authority, Cairo, Egypt. Electronic address: othman_sameh@yahoo.com.
authors.forename: Sameh H
authors.initials: SH
authors.lastname: Othman
authors.affiliation: Labeled Compound dept, Hot Laboratories Center, Atomic Energy Authority, Cairo, Egypt.
authors.forename: I A
authors.initials: IA
authors.lastname: Ibrahim
authors.affiliation: Nuclear Research Center, Atomic Energy Authority, Cairo, Egypt.
authors.forename: M H
authors.initials: MH
authors.lastname: Hatab
authors.affiliation: Radiation Research of Polymer Chemistry dept, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Elbarbary
mesh_terms: D000818:Animals; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics*; D002850:Chromatography, Gel; D005260:Female; D005492:Folic Acid / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration; D008297:Male; D008970:Molecular Weight; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D066244:Proton Magnetic Resonance Spectroscopy; D011784:Quail / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013667:Technetium / Q000493:pharmacokinetics*; D013997:Time Factors; D014001:Tin / Q000032:analysis; D014018:Tissue Distribution; D014961:X-Ray Diffraction
chemical_list: D013667:Technetium; D014001:Tin; D048271:Chitosan; D005492:Folic Acid
keywords: Biodistribution; Chitosan; Folic acid; Quail; Radiation; Radiolabeling