title: Identification of Stage-Specific Surface Markers in Early B Cell Development Provides Novel Tools for Identification of Progenitor Populations.
abstract: Whereas the characterization of B lymphoid progenitors has been facilitated by the identification of lineage- and stage-specific surface markers, the continued identification of differentially expressed proteins increases our capacity to explore normal and malignant B cell development. To identify novel surface markers with stage-specific expression patterns, we explored the reactivity of CD19(+) B cell progenitor cells to Abs targeted to 176 surface proteins. Markers with stage-specific expression were identified using a transgenic reporter gene system subdividing the B cell progenitors into four surface IgM(-) stages. This approach affirmed the utility of known stage-specific markers, as well as identifying additional proteins that selectively marked defined stages of B cell development. Among the stage-specific markers were the cell adhesion proteins CD49E, CD11A, and CD54 that are highly expressed selectively on the most immature progenitors. This work identifies a set of novel stage-specific surface markers that can be used as a complement to the classical staining protocols to explore B lymphocyte development.
authors.affiliation: Department of Molecular Hematology, Lund University, 22184 Lund, Sweden; and.
authors.forename: Christina T
authors.identifier: 0000-0002-6753-7983
authors.initials: CT
authors.lastname: Jensen
authors.affiliation: Department of Molecular Hematology, Lund University, 22184 Lund, Sweden; and.
authors.forename: Stefan
authors.identifier: 0000-0002-0854-2328
authors.initials: S
authors.lastname: Lang
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden.
authors.forename: Rajesh
authors.identifier: 0000-0003-0669-9644
authors.initials: R
authors.lastname: Somasundaram
authors.affiliation: Department of Molecular Hematology, Lund University, 22184 Lund, Sweden; and.
authors.forename: Shamit
authors.initials: S
authors.lastname: Soneji
authors.affiliation: Department of Molecular Hematology, Lund University, 22184 Lund, Sweden; and Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden mikael.sigvardsson@med.lu.se.
authors.forename: Mikael
authors.initials: M
authors.lastname: Sigvardsson
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D001854:Bone Marrow Cells / Q000276:immunology; D040881:CD11a Antigen / Q000235:genetics / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology; D002454:Cell Differentiation; D007075:Immunoglobulin M / Q000172:deficiency / Q000235:genetics; D039482:Integrin alpha5 / Q000235:genetics / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D054448:Precursor Cells, B-Lymphoid / Q000276:immunology*
chemical_list: D018941:Antigens, CD19; D040881:CD11a Antigen; D015815:Cell Adhesion Molecules; D007075:Immunoglobulin M; D039482:Integrin alpha5; D018799:Intercellular Adhesion Molecule-1