title: Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest.
abstract: Although various parts of J. curcas (Jatropha curcas L., Euphorbiaceae) have long been used as traditional folk medicines for their antiviral, analgesic, and/or antidotal efficacies, we are the first to investigate the role of anti-carcinogenicity of isoamericanol A (IAA) from the seed extract. Our results showed that IAA is capable of inhibiting cell proliferation in a dose-dependent manner on the human cancer cell lines of MCF-7, MDA-MB231, HuH-7, and HeLa. Flow cytometry analysis showed IAA significantly induces cell cycle arrest at G2/M on MCF-7 cells. At both protein and mRNA levels examined by western blot and real-time PCR, the results revealed increased expression of BTG2 (B-cell translocation gene 2), p21 (p21(WAF1/CIPI) ), and GADD45A (growth arrest and DNA-damage-inducible, alpha) after IAA treatment, but inversed expression in CDK1 (cyclin-dependent kinase 1) and cyclins B1 and B2. All these effects contribute to G2/M cell cycle arrest. Furthermore, these results coincide with the changes in molecular expressions determined by DNA-microarray analysis. Our findings indicate that IAA has an inhibitory effect on cell proliferation of MCF-7 through cell cycle arrest, giving it great potential as a future therapeutic reagent for cancers.
authors.affiliation: Department of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Katagi
authors.affiliation: Department of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 Japan.
authors.forename: Li
authors.initials: L
authors.lastname: Sui
authors.affiliation: Department of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 Japan.
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Kamitori
authors.affiliation: Laboratory of Biomass Chemistry, Biological Molecular Chemistry, Kagawa University, Faculty of Agriculture / Graduate School of Agriculture, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 Japan.
authors.forename: Toshisada
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Laboratory of Biomass Chemistry, Biological Molecular Chemistry, Kagawa University, Faculty of Agriculture / Graduate School of Agriculture, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Katayama
authors.affiliation: Department of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 Japan.
authors.forename: Akram
authors.initials: A
authors.lastname: Hossain
authors.affiliation: Department of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 Japan.
authors.forename: Chisato
authors.initials: C
authors.lastname: Noguchi
authors.affiliation: Department of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 Japan.
authors.forename: Youyi
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Yamaguchi
authors.affiliation: Department of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tokuda
keywords: Cancer; Cancer treatment; Health sciences; Pharmaceutical science; Plant biology