title: Charge Effect on the Quantum Dots-Peptide Self-Assembly Using Fluorescence Coupled Capillary Electrophoresis.
abstract: We present a molecular characterization of metal-affinity driven self-assembly between CdSe-ZnS quantum dots and a series of hexahistidine peptides with different charges. In particular, we uti- lized fluorescence coupled capillary electrophoresis to test the self-assembly process of quantum dots with peptides in solution. Four peptides with different charges can be efficiently separated by fluorescence coupled capillary electrophoresis. The migration time appeared to be influenced by the charges of the peptide. In addition, the kinetics of self-assembly process of quantum dots with one of the peptides manifested a bi-phasic kinetics followed by a saturating stage. This work revealed that there exist two types of binding sites on the surface of quantum dots for peptide 1: one type termed "high priority" binding site and a "low priority" site which is occupied after the first binding sites are fully occupied. The total self-assembly process finishes in solution within 80 s. Our work represents the systematic investigation of the details of self-assembly kinetics utilizing high-resolution fluorescence coupled capillary electrophoresis. The charge effect of peptide coating quantum dots provides a new way of preparing bioprobes.
authors.forename: Jianhao
authors.initials: J
authors.lastname: Wang
authors.forename: Jingyan
authors.initials: J
authors.lastname: Li
authors.forename: Yiwan
authors.initials: Y
authors.lastname: Teng
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Bi
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Jinchen
authors.initials: J
authors.lastname: Li
authors.forename: Cheli
authors.initials: C
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Qiu
authors.forename: Pengju
authors.initials: P
authors.lastname: Jiang
mesh_terms: D003460:Crystallization / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D008422:Materials Testing / Q000379:methods; D010455:Peptides; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*; D055672:Static Electricity
chemical_list: D010455:Peptides