title: Plasma glutamine and cystine are decreased and negatively correlated with endomysial antibody in children with celiac disease.
abstract: OBJECTIVE
  Glutamine is a nonessential amino acid which improves intestinal mucosal regeneration and absorption. Glutathione is a vital molecule for antioxidant reactions and is synthesized from cystine. The first aim of the study is to measure the plasma glutamine and cystine in children with celiac disease (CD) and compare them with controls. The second aim of this study is to investigate whether these amino acids are correlated with endomysial antibody (EMA) or not.
  
  
  METHODS
  Fifty children with CD were compared to 50 healthy, age, and sex matched normal children as control. Plasma glutamine and cystine levels of the children were measured by using tandem mass spectrometry.
  
  
  RESULTS
  Plasma glutamine (808 vs 870 μmol/L) and cystine (19 vs 48.5 μmol/L) were significantly lower in the celiac group than the controls (p<0.05). The levels of plasma glutamine (797 vs 928 μmol/L, n=42) and cystine (18 vs 31.5 μmol/L, n=8) were lower (p<0.05) in the EMA-positive than the EMA-negative celiac patients. We could not find any statistically significance between EMA-negative celiac patients and controls for the plasma glutamine (928 vs 870 μmol/L) and cystine (31.5 vs 48.5 μmol/L) (p>0.05). Serum EMA was negatively correlated with plasma cystine (r=-0,321, p=0.023), glutamine (r=-0.413, p=0.003).
  
  
  CONCLUSIONS
  Our study indicated that plasma glutamine and cystine were significantly lower in the celiac children than the controls. Also, these amino acids were negatively correlated with EMA.
authors.affiliation: Department of Pediatric Gastroenterology, Erciyes University, Kayseri, Turkey. Email: dr.eylemsevinc@gmail.com.
authors.forename: Eylem
authors.initials: E
authors.lastname: Sevinc
authors.affiliation: Department of Public Health, Erciyes University, Kayseri, Turkey.
authors.forename: Nergiz
authors.initials: N
authors.lastname: Sevinc
authors.affiliation: Department of Pediatric Allergy and Immunology, Erciyes University, Kayseri, Turkey.
authors.forename: Himmet Haluk
authors.initials: HH
authors.lastname: Akar
authors.affiliation: Department of Gastroenterology, Erciyes University, Kayseri, Turkey.
authors.forename: Banu Demet
authors.initials: BD
authors.lastname: Ozelcoskun
authors.affiliation: Department of Gastroenterology, Erciyes University, Kayseri, Turkey.
authors.forename: Gülten Can
authors.initials: GC
authors.lastname: Sezgin
authors.affiliation: Department of Pediatric Gastroenterology, Erciyes University, Kayseri, Turkey.
authors.forename: Duran
authors.initials: D
authors.lastname: Arslan
authors.affiliation: Department of Pediatric Metabolism, Erciyes University, Kayseri, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Kendirci
mesh_terms: D000293:Adolescent; D001323:Autoantibodies / Q000097:blood*; D002446:Celiac Disease / Q000097:blood* / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003553:Cystine / Q000097:blood*; D005260:Female; D005973:Glutamine / Q000097:blood*; D006801:Humans; D008297:Male
chemical_list: D001323:Autoantibodies; D005973:Glutamine; D003553:Cystine