title: Feruloyl esterase production by Aspergillus terreus CECT 2808 and subsequent application to enzymatic hydrolysis.
abstract: Ferulic acid esterases (FAE) were produced by Aspergillus terreus CECT 2808 from vine trimming shoots (VTS) and corn cob. Later, the fungal extracts thus obtained were used to enzymatically release ferulic acid (FA) from both substrates. Our findings showed a higher FAE activity in the enzymatic extracts produced on corn cob (0.070±0.004U/mL). Nevertheless, the enzymatic extracts produced on VTS demonstrated a better performance for FA release from both corn cob (2.05±0.01mg/g) and VTS (0.19±0.003mg/g). This result was probably because of the higher xylanase/FAE ratio determined in VTS extract. Therefore, an additional assay was carried out by supplementing corn cob extract with a commercial xylanase to test the influence of FAE/xylanase ratio in FA release. The results revealed the relevance of the FAE/xylanase ratio for an optimal FA release.
authors.affiliation: Department of Chemical Engineering, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, SPAIN and Laboratory of Agro-food Biotechnology, CITI (University of Vigo)-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Pérez-Rodríguez
authors.affiliation: Department of Chemical Engineering, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, SPAIN and Laboratory of Agro-food Biotechnology, CITI (University of Vigo)-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain; CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: C D
authors.initials: CD
authors.lastname: Moreira
authors.affiliation: Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Torrado Agrasar
authors.affiliation: Department of Chemical Engineering, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, SPAIN and Laboratory of Agro-food Biotechnology, CITI (University of Vigo)-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain. Electronic address: jmanuel@uvigo.es.
authors.forename: J M
authors.initials: JM
authors.lastname: Domínguez
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D013379:Substrate Specificity; D027843:Vitis / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry
chemical_list: D003373:Coumaric Acids; D005656:Fungal Proteins; C036909:lignocellulose; D008031:Lignin; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D043364:Endo-1,4-beta Xylanases
keywords: Corn cob; Enzymatic hydrolysis; Ferulic acid; Feruloyl esterase; Vine trimming shoot