title: Augmented delivery of gemcitabine in lung cancer cells exploring mannose anchored solid lipid nanoparticles.
abstract: Gemcitabine (GmcH) is an effective anti-cancer agent used in the chemotherapy of lung cancer. However, the clinical applications of GmcH has been impeded primarily due to its low blood residence time, unfavorable pharmacokinetic and pharmacodynamic (PK/PD) profile, and poor penetration in the complex environment of lung cancer cells. Thus, the present study aims to formulate GmcH loaded mannosylated solid lipid nanoparticles (GmcH-SLNs) for improving its drug uptake into the lung cancer cells. GmcH-SLNs were prepared by emulsification and solvent evaporation process, and surface modification was done with mannose using ring opening technique. The cellular toxicity and cell uptake studies were performed in A549 lung adenocarcinoma cell line. The developed nanoformulation appears to be proficient in targeted delivery of GmcH with improved therapeutic effectiveness and enhanced safety.
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Deemed University), Allahabad, UP, India.
authors.forename: Namrata
authors.initials: N
authors.lastname: Soni
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Deemed University), Allahabad, UP, India.
authors.forename: Neetu
authors.initials: N
authors.lastname: Soni
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Deemed University), Allahabad, UP, India.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Pandey
authors.affiliation: Department of Pharmaceutics, BM College of Pharmaceutical Education & Research, Indore 452010, MP, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Maheshwari
authors.affiliation: Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 259 Mack Avenue, Detroit, MI 48201, United States.
authors.forename: Prashant
authors.initials: P
authors.lastname: Kesharwani
authors.affiliation: The International Medical University, School of Pharmacy, Department of Pharmaceutical Technology, Jalan Jalil Perkasa 19, 57000 Kuala Lumpur, Malaysia; National Institute of Pharmaceutical Education and Research (NIPER), Sarkhej - Gandhinagar Highway, Thaltej, Ahmedabad 380054, Gujarat, India. Electronic address: rakeshtekade@gmail.com.
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Tekade
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000493:pharmacokinetics* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D008358:Mannose / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D013329:Structure-Activity Relationship; D000093542:Gemcitabine
chemical_list: D000964:Antimetabolites, Antineoplastic; D008055:Lipids; D003841:Deoxycytidine; D008358:Mannose; D000093542:Gemcitabine
keywords: Drug delivery; Gemcitabine; Lung cancer; Solid lipid nanoparticles