title: Occurrence and characterisation of calcium oxalate crystals in stems and fruits of Hylocereus costaricensis and Selenicereus megalanthus (Cactaceae: Hylocereeae).
abstract: Detailed description about occurrence of calcium oxalate (CaOx) crystals in the edible vine cactus species Hylocereus costaricensis and Selenicereus megalanthus is scarce. Therefore, we evaluated and characterized the presence, morphology and composition of CaOx crystals in both species. Crystals were isolated from greenhouse and in vitro vegetative stems, and from ripe fruit peels and pulp by enzymatic digestion and density centrifugation and quantified with a haemocytometer. Morphologies were studied using scanning electron microscopy, elemental composition with energy-dispersive X-ray spectroscopy and salt composition with X-ray powder diffraction. Analyses conducted confirmed that isolated crystals were exclusively composed by CaOx, both mono- and dihydrated. Highest crystal contents were measured in greenhouse stems, followed by the fruit peels. While very few crystals were quantified in in vitro plants, they were not detected in the fruit pulp at all, which is of advantage for its human consumption and could be linked to mechanisms of seed dispersal through animals. Different crystal morphologies were observed, sometimes varying between genotypes and tissues analysed. This is the first work known to the authors with a detailed characterization of CaOx crystals in vine cacti.
authors.affiliation: CIGRAS and CIEMic, Universidad de Costa Rica, 2060 San Pedro, Costa Rica. Electronic address: mavime7@gmail.com.
authors.forename: María
authors.initials: M
authors.lastname: Viñas
authors.affiliation: CIGRAS and CIEMic, Universidad de Costa Rica, 2060 San Pedro, Costa Rica; Food Security Center, University of Hohenheim, 70599 Stuttgart, Germany. Electronic address: victor.jimenez@ucr.ac.cr.
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Jiménez
mesh_terms: D029421:Cactaceae / Q000033:anatomy & histology / Q000737:chemistry*; D002129:Calcium Oxalate / Q000032:analysis / Q000737:chemistry*; D003460:Crystallization; D005638:Fruit / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D018547:Plant Stems / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D014961:X-Ray Diffraction
chemical_list: D002129:Calcium Oxalate
keywords: Calcium oxalate crystal; Elemental composition; Energy-dispersive X-ray spectroscopy; Pitahaya; Scanning electron microscopy; X-ray powder diffraction