title: Expression Pattern of Mouse Vasa Homologue (MVH) in the Ovaries of C57BL/6 Female Mice.
abstract: BACKGROUND Vasa (a DEAD-box helicase, also known as Ddx4) is an ATP-dependent RNA helicase highly conserved among all animals. Research on the presence and function of DDX4 in female mammals is limited. To gain greater insight into its distribution and role in female mice, we detected the expression of DDX4 protein in the ovaries and analyzed its expression pattern. MATERIAL AND METHODS MVH was detected in the cytoplasm of oocytes in all non-apoptotic follicles. RESULTS In the present study, we found that higher expression levels of ~55-60 kDa MVH isoform in the ovaries were followed by the accumulations of preovulatory follicles. CONCLUSIONS Higher levels of MVH protein in the ovaries might prepare oocytes for the competence to resume meiosis.
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan, Hubei, China (mainland).
authors.forename: Kunkun
authors.initials: K
authors.lastname: Song
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan, Hubei, China (mainland).
authors.forename: Wenwen
authors.initials: W
authors.lastname: Ma
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan, Hubei, China (mainland).
authors.forename: Cong
authors.initials: C
authors.lastname: Huang
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan, Hubei, China (mainland).
authors.forename: Jiahui
authors.initials: J
authors.lastname: Ding
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan, Hubei, China (mainland).
authors.forename: Dandan
authors.initials: D
authors.lastname: Cui
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan, Hubei, China (mainland).
authors.forename: Mingmin
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D053487:DEAD-box RNA Helicases / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000378:metabolism; D010053:Ovary / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; C090864:Ddx4 protein, mouse; D053487:DEAD-box RNA Helicases