title: Attribution of NKG2DL to the inhibition of early stage allogeneic tumors in mice.
abstract: Allogeneic tumors are eventually rejected by adaptive immune responses, however, little is known about how allogeneic tumors are eradicated at the early stage of tumor development. In present study, we found that NKG2DL low expressing cancer cells were developed into palpable allogeneic tumors in mice within a week after the inoculation, while NKG2DL high expressing cancer cells failed to. The NKG2DL high expressing cancer cells could increase NKG2D+ NK cells in the allogeneic mice after being inoculated for 3 days. Artificially up-regulating NKG2DL on cancer cells with low level expressed NKG2DL by a CpG ODN resulted in the retardation and rejection of the allogeneic tumors at the early stage. The contribution of up-regulated NKG2DL to the early rejection was further confirmed by the results that the development of allogeneic tumors from cancer cells transfected with NKG2DL genes was significantly inhibited in mice at the early stage. Overall, hopefully, the data may provide insights for combining the allogeneic NK cell adoptive transfer with the approaches of up-regulating NKG2DL to treat cancer patients.
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Li
authors.initials: L
authors.lastname: Hua
authors.affiliation: Department of Molecular Biology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Mingli
authors.initials: M
authors.lastname: Fang
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Boqi
authors.initials: B
authors.lastname: Dong
authors.affiliation: Department of Molecular Biology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Cuiyun
authors.initials: C
authors.lastname: Cui
authors.affiliation: Department of Molecular Biology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Molecular Biology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Molecular Biology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Molecular Biology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Molecular Biology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Yunjia
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Hao
authors.affiliation: Department of Molecular Biology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Peiyan
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Yilan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Molecular Biology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D005910:Glioma / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008546:Melanoma, Experimental / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008565:Membrane Proteins; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D009368:Neoplasm Transplantation; D015398:Signal Transduction; D012878:Skin Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D014162:Transfection; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic; D047368:Tumor Burden
chemical_list: D002352:Carrier Proteins; D015395:Histocompatibility Antigens Class I; C527086:Klrk1 protein, mouse; D008565:Membrane Proteins; D055655:NK Cell Lectin-Like Receptor Subfamily K; C468260:UL16 binding protein 1, mouse
keywords: NK cells; NKG2D+ cells; NKG2DL; allogeneic tumors; early stage