title: A facile label-free aptasensor for detecting ATP based on fluorescence enhancement of poly(thymine)-templated copper nanoparticles.
abstract: A label-free fluorescence assay has been developed for sensitive and selective detection of adenosine triphosphate (ATP) by using poly(thymine) (poly T)-templated copper nanoparticles (CuNPs) as fluorescent indicator. In our design, ATP aptamer was split into two fragments, both of which were elongated with poly T strands that can be utilized as efficient template for the formation of copper nanoparticles through the reduction of copper ions by sodium ascorbate. In the presence of ATP, the two split aptamers could be dragged to form aptamer-ATP aptamer complex, which drew the poly T strands close to each other and induced a remarkable fluorescence enhancement of poly T-templated CuNPs. Thus, an elevated fluorescence enhancement of poly T-templated CuNPs was obtained with the increase in ATP concentration. Under optimized conditions, a good linear range for ATP detection was realized from 100 nM to 100 μM with a detection limit of 10.29 nM. In addition, the application of this biosensing system in complex biological matrix was demonstrated with satisfactory results. This assay provided a simple, label-free, cost-effective, and sensitive platform for the detection of ATP.
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, No. 100 Science Road, Zhengzhou, Henan, 450001, China.
authors.forename: Sai-Sai
authors.initials: SS
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, No. 100 Science Road, Zhengzhou, Henan, 450001, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, No. 100 Science Road, Zhengzhou, Henan, 450001, China.
authors.forename: Qi-Yong
authors.initials: QY
authors.lastname: Cai
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, No. 100 Science Road, Zhengzhou, Henan, 450001, China.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Dong
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, No. 100 Science Road, Zhengzhou, Henan, 450001, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Geng
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, No. 100 Science Road, Zhengzhou, Henan, 450001, China. jiage0630@hnu.edu.cn.
authors.forename: Jia
authors.initials: J
authors.lastname: Ge
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, No. 100 Science Road, Zhengzhou, Henan, 450001, China. zhaohui.li@zzu.edu.cn.
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Li
mesh_terms: D000072283:A549 Cells; D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013941:Thymine / Q000031:analogs & derivatives*
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D003300:Copper; D000255:Adenosine Triphosphate; D013941:Thymine
keywords: ATP detection; Label-free; Poly(thymine)-templated CuNPs; Split aptamers