title: Microfluidic microscopy-assisted label-free approach for cancer screening: automated microfluidic cytology for cancer screening.
abstract: Each year, about 7-8 million deaths occur due to cancer around the world. More than half of the cancer-related deaths occur in the less-developed parts of the world. Cancer mortality rate can be reduced with early detection and subsequent treatment of the disease. In this paper, we introduce a microfluidic microscopy-based cost-effective and label-free approach for identification of cancerous cells. We outline a diagnostic framework for the same and detail an instrumentation layout. We have employed classical computer vision techniques such as 2D principal component analysis-based cell type representation followed by support vector machine-based classification. Analogous to criminal face recognition systems implemented with help of surveillance cameras, a signature-based approach for cancerous cell identification using microfluidic microscopy surveillance is demonstrated. Such a platform would facilitate affordable mass screening camps in the developing countries and therefore help decrease cancer mortality rate.
authors.affiliation: Optics and Microfluidics Instrumentation Lab, Department of Instrumentation and Applied Physics, Indian Institute of Science, Malleshwaram, Bangalore, 560012, India.
authors.forename: Veerendra Kalyan
authors.initials: VK
authors.lastname: Jagannadh
authors.affiliation: Department of Earth and Space Sciences, Indian Institute of Space Science and Technology, Thiruvananthapuram, Kerala, 695547, India.
authors.forename: G
authors.initials: G
authors.lastname: Gopakumar
authors.affiliation: Department of Earth and Space Sciences, Indian Institute of Space Science and Technology, Thiruvananthapuram, Kerala, 695547, India.
authors.forename: Gorthi R K Sai
authors.initials: GRKS
authors.lastname: Subrahmanyam
authors.affiliation: Optics and Microfluidics Instrumentation Lab, Department of Instrumentation and Applied Physics, Indian Institute of Science, Malleshwaram, Bangalore, 560012, India. saisiva.gorthi@iap.iisc.ernet.in.
authors.forename: Sai Siva
authors.initials: SS
authors.lastname: Gorthi
mesh_terms: D055088:Early Detection of Cancer / Q000379:methods*; D006801:Humans; D008403:Mass Screening / Q000379:methods; D044085:Microfluidics / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis*
keywords: Cancer diagnostics; Cell identification; High-throughput imaging; Optofluidic imaging