title: Structural identification of triacylglycerol isomers using electron impact excitation of ions from organics (EIEIO).
abstract: Electron-induced dissociation or electron impact excitation of ions from organics (EIEIO) was applied to triacylglycerols (TAGs) for in-depth molecular structure analysis using MS. In EIEIO, energetic electrons (∼10 eV) fragmented TAG ions to allow for regioisomeric assignment of identified acyl groups at the sn-2 or sn-1/3 positions of the glycerol backbone. In addition, carbon-carbon double bond locations within the acyl chains could also be assigned by EIEIO. Beyond the analysis of lipid standards, this technique was applied to edible oils and natural lipid extracts to demonstrate the power of this method to provide in-depth structural elucidation of TAG molecular species.
authors.affiliation: SCIEX, Concord, Ontario, Canada takashi.baba@sciex.com.
authors.forename: Takashi
authors.initials: T
authors.lastname: Baba
authors.affiliation: SCIEX, Concord, Ontario, Canada.
authors.forename: J Larry
authors.initials: JL
authors.lastname: Campbell
authors.affiliation: SCIEX, Concord, Ontario, Canada.
authors.forename: J C Yves
authors.initials: JC
authors.lastname: Le Blanc
authors.affiliation: SCIEX, Redwood Shores, CA.
authors.forename: Paul R S
authors.initials: PR
authors.lastname: Baker
mesh_terms: D004583:Electrons; D005990:Glycerol / Q000737:chemistry*; D007477:Ions / Q000737:chemistry*; D007536:Isomerism; D008055:Lipids / Q000737:chemistry*; D013058:Mass Spectrometry; D010938:Plant Oils / Q000737:chemistry; D014280:Triglycerides / Q000737:chemistry*
chemical_list: D007477:Ions; D008055:Lipids; D010938:Plant Oils; D014280:Triglycerides; D005990:Glycerol
keywords: electron induced dissociation; fish oil; lipidomics; lipids/chemistry; mass spectrometry; triglycerides