title: Resveratrol mitigates hepatic injury in rats by regulating oxidative stress, nuclear factor-kappa B, and apoptosis.
abstract: Resveratrol is a naturally occurring polyphenol, possesses several pharmacological activities including anticancer, antioxidant, antidiabetic, antinociceptive, and antiasthmatic activity. Little is known about its hepatoprotective action mechanisms. This study was conceived to explore the possible protective mechanisms of resveratrol compared with the hepatoprotective silymarin in thioacetamide (TAA)-induced hepatic injury in rats. Thirty-two rats were equally divided into four groups; normal control (i), TAA (100 mg/kg) (ii), TAA + silymarin (50 mg/kg) (iii), and TAA + resveratrol (10 mg/kg) (iv). Liver function and histopathology, pro-inflammatory cytokines, oxidative stress, and apoptotic markers were examined. Data were analyzed using ANOVA test followed by Tukey post hoc test. Compared to TAA-intoxicated group, resveratrol mitigated liver damage, and inflammation as noted by less inflammatory infiltration, hydropic degeneration with decreased levels of tumor necrosis factor-alpha, interleukin-6, and interferon-gamma by 78.83, 18.12, and 64.49%, respectively. Furthermore, it reduced (P < 0.05) alanine and aspartate aminotransferases by 36.64 and 48.09%, respectively, restored hepatic glutathione content and normalized superoxide dismutase and malondialdehyde levels. While it inhibited nuclear factor-kappa B, cytochrome 2E1, and enhanced apoptosis of necrotic hepatocytes via increasing caspase-3 activity. Our findings indicated that the potential hepatoprotective mechanisms of resveratrol are associated with inhibition of inflammation, enhancing the apoptosis of necrotic hepatocytes, and suppression of oxidative stress.
authors.affiliation: Department of Pharmacology, Theodor Bilharz Research Institute, Warak El-Hadar, Imbaba, Giza 12411, Egypt.
authors.forename: Sayed Hassan
authors.initials: SH
authors.lastname: Seif El-Din
authors.affiliation: Department of Pharmacology, Theodor Bilharz Research Institute, Warak El-Hadar, Imbaba, Giza 12411, Egypt.
authors.forename: Naglaa Mohamed
authors.initials: NM
authors.lastname: El-Lakkany
authors.affiliation: Department of Pharmacology, Theodor Bilharz Research Institute, Warak El-Hadar, Imbaba, Giza 12411, Egypt.
authors.forename: Maha Badr
authors.initials: MB
authors.lastname: Salem
authors.affiliation: Department of Pathology, Theodor Bilharz Research Institute, Warak El-Hadar, Imbaba, Giza 12411, Egypt.
authors.forename: Olfat Ali
authors.initials: OA
authors.lastname: Hammam
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.
authors.forename: Samira
authors.initials: S
authors.lastname: Saleh
authors.affiliation: Department of Pharmacology, Theodor Bilharz Research Institute, Warak El-Hadar, Imbaba, Giza 12411, Egypt.
authors.forename: Sanaa Sabet
authors.initials: SS
authors.lastname: Botros
keywords: Caspase-3; cytotoxicity; liver inflammation; pro-inflammatory cytokines; resveratrol