title: The plasma 8-OHdG levels and oxidative stress following cholecystectomy: a randomised multicentre study of patients with minilaparotomy cholecystectomy versus laparoscopic cholecystectomy.
abstract: OBJECTIVE
  The aim of the study was to evaluate the role of 8-OHdG (8-hydroxy-2'-deoxyguanosine) detecting oxidative stress response following cholecystectomy in a randomised multicentre study of patients with minilaparotomy cholecystectomy (MC) versus laparoscopic cholecystectomy (LC).
  
  
  METHODS
  Initially, 106 patients with non-complicated symptomatic gallstone disease were randomised into MC (n = 56) or LC (n = 50) groups. Plasma levels of the oxidative stress marker 8-OHdG measured at three time points; before (PRE), immediately after (POP1) and 6 h after operation (POP2).
  
  
  RESULTS
  The demographic variables and the surgical data were similar in the study groups. The plasma oxidative stress marker 8-OHdG concentrations following surgery in the MC versus LC patients were quite similar. There was no significant correlation between the individual values of the11-point numeric rating pain scale (NRS) versus the plasma 8-OHdG post-operatively in the MC and LC patients. However, there was a statistically significant correlation between the individual values of the plasma 8-OHdG (PRE) versus IL-10 (PRE) for the MC and LC patients (r = 0.214, p = 0.037). There was also a statistically significant correlation between the individual values of the plasma 8-OHdG (POP2) versus IL-1β (POP2) for the MC and LC patients (r = 0.25, p = 0.01).
  
  
  CONCLUSIONS
  Our results suggest that the oxidative stress marker 8-OHdG concentrations following surgery in MC versus LC patients were quite similar. A new finding with possible clinical relevance is a correlation between the individual plasma values of the 8-OHdG versus anti-inflammatory interleukin IL-10 and 8-OHdG versus IL-1β (proinflammatory) in the MC and LC patients suggesting that inflammation and oxidative stress are related.
authors.affiliation: a Department of Surgery , Kuopio University Hospital and School of Medicine, University of Eastern , Kuopio , Finland.
authors.forename: Samuli
authors.initials: S
authors.lastname: Aspinen
authors.affiliation: b Department of Surgery , Helsinki University Central Hospital , Helsinki , Finland.
authors.forename: Jukka
authors.initials: J
authors.lastname: Harju
authors.affiliation: a Department of Surgery , Kuopio University Hospital and School of Medicine, University of Eastern , Kuopio , Finland.
authors.forename: Petri
authors.initials: P
authors.lastname: Juvonen
authors.affiliation: c Science Service Centre, Kuopio University Hospital, University of Eastern Finland , Kuopio , Finland.
authors.forename: Tuomas
authors.initials: T
authors.lastname: Selander
authors.affiliation: d Department of Anaesthesia and Operative Services , Kuopio University Hospital and School of Medicine, University of Eastern Finland , Kuopio , Finland.
authors.forename: Hannu
authors.initials: H
authors.lastname: Kokki
authors.affiliation: e Department of Clinical Chemistry , Institute of Clinical Medicine, University of Eastern Finland and Eastern Finland Laboratory Centre , Kuopio , Finland.
authors.forename: Kari
authors.initials: K
authors.lastname: Pulkki
authors.affiliation: a Department of Surgery , Kuopio University Hospital and School of Medicine, University of Eastern , Kuopio , Finland.
authors.forename: Matti Johannes
authors.initials: MJ
authors.lastname: Eskelinen
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000097:blood; D017081:Cholecystectomy, Laparoscopic / Q000009:adverse effects / Q000379:methods*; D002769:Cholelithiasis / Q000097:blood* / Q000601:surgery*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D005387:Finland; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010149:Postoperative Pain / Q000209:etiology; D055815:Young Adult
chemical_list: D015415:Biomarkers; D053583:Interleukin-1beta; D016753:Interleukin-10; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: Cholecystectomy; laparoscopy; minilaparotomy; oxidative response