title: Evaluation of potential genotoxic/cytotoxic effects induced by epoxiconazole and fenpropimorph-based fungicide in bovine lymphocytes in vitro.
abstract: Potential genotoxic/cytotoxic effects of the epoxiconazole/fenpropimorph-based fungicide were investigated using single cell gel electrophoresis and cytogenetic assays: chromosomal aberrations, sister chromatid exchanges, micronuclei and fluorescence in situ hybridization in cultured bovine lymphocytes. No statistically significant elevations of DNA damage and increases in cytogenetic endpoints were seen. However, evident cytotoxic effect presented as a decrease in mitotic and proliferation indices were recorded after exposure of bovine lymphocytes to the fungicide for 24 and 48 h at concentrations ranging from 3 to 15 µg mL(-1) (P < 0.05, P < 0.01, P < 0.001). Similarly, for 24 h an inhibition in the cytokinesis block proliferation index (CBPI) was obtained after exposure to the fungicide at concentrations ranging from 1.5 to 15 µg mL(-1) (P < 0.01, P < 0.001) in each donor.
authors.affiliation: a Department of Biology and Genetics , University of Veterinary Medicine and Pharmacy in Košice , Košice , Slovak Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Drážovská
authors.affiliation: a Department of Biology and Genetics , University of Veterinary Medicine and Pharmacy in Košice , Košice , Slovak Republic.
authors.forename: Katarína
authors.initials: K
authors.lastname: Šiviková
authors.affiliation: a Department of Biology and Genetics , University of Veterinary Medicine and Pharmacy in Košice , Košice , Slovak Republic.
authors.forename: Beáta
authors.initials: B
authors.lastname: Holečková
authors.affiliation: a Department of Biology and Genetics , University of Veterinary Medicine and Pharmacy in Košice , Košice , Slovak Republic.
authors.forename: Ján
authors.initials: J
authors.lastname: Dianovský
authors.affiliation: a Department of Biology and Genetics , University of Veterinary Medicine and Pharmacy in Košice , Košice , Slovak Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Galdíková
authors.affiliation: a Department of Biology and Genetics , University of Veterinary Medicine and Pharmacy in Košice , Košice , Slovak Republic.
authors.forename: Viera
authors.initials: V
authors.lastname: Schwarzbacherová
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured / Q000187:drug effects; D002869:Chromosome Aberrations / Q000187:drug effects; D003603:Cytotoxins / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects; D004852:Epoxy Compounds / Q000633:toxicity*; D005659:Fungicides, Industrial / Q000494:pharmacology / Q000633:toxicity*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000187:drug effects*; D008940:Mitotic Index; D009025:Morpholines / Q000633:toxicity*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects; D014230:Triazoles / Q000633:toxicity*
chemical_list: D003603:Cytotoxins; D004852:Epoxy Compounds; D005659:Fungicides, Industrial; D009025:Morpholines; D009153:Mutagens; D014230:Triazoles
keywords: Conazoles; chromosomal aberrations; genotoxic/cytotoxic effect; micronucleus; single cell gel electrophoresis; sister chromatid exchanges