title: A step change in the transfer of interspecific variation into wheat from Amblyopyrum muticum.
abstract: Despite some notable successes, only a fraction of the genetic variation available in wild relatives has been utilized to produce superior wheat varieties. This is as a direct result of the lack of availability of suitable high-throughput technologies to detect wheat/wild relative introgressions when they occur. Here, we report on the use of a new SNP array to detect wheat/wild relative introgressions in backcross progenies derived from interspecific hexaploid wheat/Ambylopyrum muticum F1hybrids. The array enabled the detection and characterization of 218 genomewide wheat/Am. muticum introgressions, that is a significant step change in the generation and detection of introgressions compared to previous work in the field. Furthermore, the frequency of introgressions detected was sufficiently high to enable the construction of seven linkage groups of the Am. muticum genome, thus enabling the syntenic relationship between the wild relative and hexaploid wheat to be determined. The importance of the genetic variation from Am. muticum introduced into wheat for the development of superior varieties is discussed.
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
authors.forename: Julie
authors.initials: J
authors.lastname: King
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
authors.forename: Surbhi
authors.initials: S
authors.lastname: Grewal
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
authors.forename: Cai-Yun
authors.initials: CY
authors.lastname: Yang
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
authors.forename: Stella
authors.initials: S
authors.lastname: Hubbart
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
authors.forename: Duncan
authors.initials: D
authors.lastname: Scholefield
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
authors.forename: Stephen
authors.initials: S
authors.lastname: Ashling
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Edwards
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Allen
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Amanda
authors.initials: A
authors.lastname: Burridge
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Claire
authors.initials: C
authors.lastname: Bloor
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Davassi
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, UK.|Federal University of Pelotas, Pelotas, Brazil.
authors.forename: Glacy J
authors.initials: GJ
authors.lastname: da Silva
authors.affiliation: School of Agriculture, Food and Wine, The University of Adelaide, Adelaide, SA, Australia.
authors.forename: Ken
authors.initials: K
authors.lastname: Chalmers
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
authors.forename: Ian P
authors.initials: IP
authors.lastname: King
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D008040:Genetic Linkage; D014644:Genetic Variation; D018745:Genome, Plant; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods; D009693:Nucleic Acid Hybridization; D006109:Poaceae / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D026801:Synteny; D014908:Triticum / Q000235:genetics*
keywords: Introgression; Wheat; genotyping; synteny; wild relatives