title: Decreased Dp71 expression is associated with gastric adenocarcinoma prognosis.
abstract: For the first time, dramatically decreased Dp71 protein and mRNA was found in 34 pairs of resected primary gastric adenocarcinoma. Immunohistochemistry identified Dp71 expression suppressed in 72.2% of 104 gastric cancer patients. The decreased Dp71 expression was significantly correlated with cancer differentiation (P=0.001) and lymph vascular invasion (p=0.041). Decreased Dp71 expression was associated with a poor gastric adenocarcinoma prognosis (P=0.001). Significantly less Dp71 mRNA and protein were found in BGC823, SGC7901, AGS compared with GES-1. Via increasing lamin B1 mRNA and protein, enforced Dp71d and Dp71f expression resulted in SGC7901 proliferation inhibition. Co-IP proved interaction of Dp71 with lamin B1 in GES-1 cells. Further expression characterization showed reduced lamin B1 in gastric cancer tissue and cancer cells. Increasing lamin B1 expression results in the growth inhibition of SGC7901, which suggests that Dp71-lamin B1 protein complex plays an important role for the newly identified tumor suppressive function of Dp71.
authors.affiliation: Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, People's Republic of China.
authors.forename: Sipin
authors.initials: S
authors.lastname: Tan
authors.affiliation: Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Tan
authors.affiliation: Department of Thoracic Surgery, Second Xiangya Hospital, Central South University, Hunan 410011, People's Republic of China.
authors.forename: Sichuang
authors.initials: S
authors.lastname: Tan
authors.affiliation: Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, People's Republic of China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, People's Republic of China.
authors.forename: Zhikang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, People's Republic of China.
authors.forename: Qiaocheng
authors.initials: Q
authors.lastname: Weng
authors.affiliation: Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, People's Republic of China.
authors.forename: Huali
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, People's Republic of China.
authors.forename: Kangkai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, People's Republic of China.
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D016189:Dystrophin / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D013274:Stomach Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D016189:Dystrophin; C085339:apo-dystrophin 1
keywords: Dp71; gastric cancer; gastric cancer cells; lamin B1; prognosis