title: Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis.
abstract: We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other OCT-based techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions.
authors.affiliation: Institut Langevin-ESPCI ParisTech, PSL Research University 1 Rue Jussieu, 75005, Paris, France; LLTech SAS, Pépinière Paris Santé Cochin 29 Rue du Faubourg Saint Jacques, 75014, Paris, France.
authors.forename: Clement
authors.initials: C
authors.lastname: Apelian
authors.affiliation: LLTech SAS, Pépinière Paris Santé Cochin 29 Rue du Faubourg Saint Jacques, 75014, Paris, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Harms
authors.affiliation: Institut Langevin-ESPCI ParisTech, PSL Research University 1 Rue Jussieu, 75005, Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Thouvenin
authors.affiliation: Institut Langevin-ESPCI ParisTech, PSL Research University 1 Rue Jussieu, 75005, Paris, France; LLTech SAS, Pépinière Paris Santé Cochin 29 Rue du Faubourg Saint Jacques, 75014, Paris, France.
authors.forename: A Claude
authors.initials: AC
authors.lastname: Boccara
keywords: (100.0100) Image processing; (110.4500) Optical coherence tomography; (110.6915) Time imaging; (170.1530) Cell analysis; (170.6930) Tissue; (180.6900) Three-dimensional microscopy