title: Capillary electrophoresis hyphenated with UV-native-laser induced fluorescence detection (CE/UV-native-LIF).
abstract: Native laser-induced fluorescence using UV lasers associated to CE offers now a large related literature, for now 30 years. The main works have been performed using very expensive Ar-ion lasers emitting at 257 and 275 nm. They are not affordable for routine analyses, but have numerous applications such as protein, catecholamine, and indolamine analysis. Some other lasers such as HeCd 325 nm have been used but only for few applications. Diode lasers, emitting at 266 nm, cheaper, are extensively used for the same topics, even if the obtained sensitivity is lower than the one observed using the costly UV-Ar-ion lasers. This review presents various CE or microchips applications and different UV lasers used for the excitation of native fluorescence. We showed that CE/Native UV laser induced fluorescence detection is very sensitive for detection as well as small aromatic biomolecules than proteins containing Trp and Tyr amino acids. Moreover, it is a simple way to analyze biomolecules without derivatization.
authors.affiliation: Laboratoire des IMRCP, Université de Toulouse, Université Paul Sabatier, TOULOUSE Cedex 04, France.
authors.forename: François
authors.initials: F
authors.lastname: Couderc
authors.affiliation: Laboratoire des IMRCP, Université de Toulouse, Université Paul Sabatier, TOULOUSE Cedex 04, France.
authors.forename: Varravaddheay
authors.initials: V
authors.lastname: Ong-Meang
authors.affiliation: Laboratoire des IMRCP, Université de Toulouse, Université Paul Sabatier, TOULOUSE Cedex 04, France.
authors.forename: Véréna
authors.initials: V
authors.lastname: Poinsot
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D002395:Catecholamines / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D007834:Lasers; D018377:Neurotransmitter Agents / Q000032:analysis*; D011506:Proteins / Q000032:analysis; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
chemical_list: D000596:Amino Acids; D002395:Catecholamines; D018377:Neurotransmitter Agents; D011506:Proteins
keywords: Biomolecules; Capillary electrophoresis; Catecholamine; Native laser-induced fluorescence; Proteins