title: Genetic Manipulations in Dermatophytes.
abstract: Dermatophytes are a group of closely related fungi that nourish on keratinized materials for their survival. They infect stratum corneum, nails, and hair of human and animals, accounting the largest portion of fungi causing superficial mycoses. Huge populations are suffering from dermatophytoses, though the biology of these fungi is largely unknown yet. Reasons are partially attributed to the poor amenability of dermatophytes to genetic manipulation. However, advancements in this field over the last decade made it possible to conduct genetic studies to satisfying extents. These included genetic transformation methods, indispensable molecular tools, i.e., dominant selectable markers, inducible promoter, and marker recycling system, along with improving homologous recombination frequency and gene silencing. Furthermore, annotated genome sequences of several dermatophytic species have recently been available, ensuring an optimal recruitment of the molecular tools to expand our knowledge on these fungi. In conclusion, the establishment of basic molecular tools and the availability of genomic data will open a new era that might change our understanding on the biology and pathogenicity of this fungal group.
authors.affiliation: Laboratory of Space and Environmental Medicine, Graduate School of Medicine, Teikyo University, 2-11-1, Kaga, Itabashi, Tokyo, 173-8605, Japan.
authors.forename: Mohamed Mahdi
authors.initials: MM
authors.lastname: Alshahni
authors.affiliation: Teikyo University Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo, 192-0395, Japan. tsyamada@main.teikyo-u.ac.jp.
authors.forename: Tsuyoshi
authors.identifier: http://orcid.org/0000-0002-1394-5455
authors.initials: T
authors.lastname: Yamada
mesh_terms: D003883:Arthrodermataceae / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D005827:Genetics, Microbial / Q000379:methods*; D006801:Humans; D008967:Molecular Biology / Q000379:methods*
keywords: Copper-repressible promoter; Dermatophytes; Genetic manipulation; Genetic transformation; Homologous recombination; Marker recycling; RNA interference