title: A Novel Cell Line (NCU-L-1) from a Patient with Acute Lymphoblastic Leukemia, FAB, L3, Burkitt's Type.
abstract: A novel cultured cell line, NCU-L-1, was established from a 71-year-old Japanese woman with acute lymphoblastic leukemia, L3 FAB, Burkitt's type. The NCU-L-1 cells were shown to have a mature B-cell phenotype on the basis of immunologic surface marker analysis; including IgG Lambda surface immunoglobulins, CD19, CD20 and la-like antigen which were all detected on the cells. Intracytoplasmic immunoglobulin was not detected, but IgG was present in the cell culture supernatant. Cytogenetic studies revealed that the NCU-L-1 cells had t(2; 8) and an additional 14q+, a genotype which has not been identified previously in the usual Burkitt's cell lines. The NCU-L-1 cell line should prove to be useful for studying oncogenic events associated with the t(2; 8) translocation and karyotype evolution.
authors.affiliation: a The Second Department of Internal Medicine, Nagoya City University Medical School Nagoya, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nitta
authors.affiliation: a The Second Department of Internal Medicine, Nagoya City University Medical School Nagoya, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Iwaki
authors.affiliation: a The Second Department of Internal Medicine, Nagoya City University Medical School Nagoya, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Wakita
authors.affiliation: a The Second Department of Internal Medicine, Nagoya City University Medical School Nagoya, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kodama
authors.affiliation: a The Second Department of Internal Medicine, Nagoya City University Medical School Nagoya, Japan.
authors.forename: G
authors.initials: G
authors.lastname: Takeuchi
authors.affiliation: a The Second Department of Internal Medicine, Nagoya City University Medical School Nagoya, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takada
authors.affiliation: a The Second Department of Internal Medicine, Nagoya City University Medical School Nagoya, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Mitomo
authors.affiliation: a The Second Department of Internal Medicine, Nagoya City University Medical School Nagoya, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.affiliation: b Department of Internal Medicine, Tosei Hospital Nagoya, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Amano
authors.affiliation: c The First Department of Internal Medicine, Nagoya University, School of Medicine Nagoya, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Murate
authors.affiliation: d Aichi Cancer Center Fujita-Gakuen Health University, School of Medicine, Nagoya, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Koike
authors.affiliation: d Aichi Cancer Center Fujita-Gakuen Health University, School of Medicine, Nagoya, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Osada
authors.affiliation: d Aichi Cancer Center Fujita-Gakuen Health University, School of Medicine, Nagoya, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Ueda
authors.affiliation: e Department of Pathological Cytology, Fujita-Gakuen Health University, School of Medicine, Nagoya, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shamoto
keywords: 14q+; Acute lymphoblastic leukemia; L3 (Burkitt's type); cell line; t(2; 8) translocation