title: Comparison of the Hemagglutination Inhibition Test and IgG ELISA in Categorizing Primary and Secondary Dengue Infections Based on the Plaque Reduction Neutralization Test.
abstract: Secondary dengue infection by heterotypic serotypes is associated with severe manifestations of disease, that is, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The World Health Organization (WHO) has recommended criteria based on the hemagglutination inhibition (HI) test to distinguish between primary and secondary dengue infections. Since the HI test has practical limitations and disadvantages, we evaluated the accuracy of WHO HI criteria and compared it with criteria based on an IgG enzyme-linked immunosorbent assay (ELISA) using a plaque reduction neutralization test (PRNT) as the gold standard. Both WHO HI criteria and IgG ELISA criteria performed strongly (16/16) in determining primary infection. However, to determine secondary infection, the IgG ELISA criteria performed better (72/73) compared to the WHO HI criteria (23/73).
authors.affiliation: Indonesia Research Partnership on Infectious Diseases (INA-RESPOND), Jalan Percetakan Negara No. 29, Jakarta 10560, Indonesia; U.S. Naval Medical Research Unit No. 2, Jakarta 10560, Indonesia.
authors.forename: Nurhayati
authors.initials: N
authors.lastname: Lukman
authors.affiliation: U.S. Naval Medical Research Unit No. 2, Jakarta 10560, Indonesia.
authors.forename: Gustiani
authors.identifier: 0000-0002-8940-4428
authors.initials: G
authors.lastname: Salim
authors.affiliation: Indonesia Research Partnership on Infectious Diseases (INA-RESPOND), Jalan Percetakan Negara No. 29, Jakarta 10560, Indonesia; U.S. Naval Medical Research Unit No. 2, Jakarta 10560, Indonesia.
authors.forename: Herman
authors.identifier: 0000-0003-1095-3422
authors.initials: H
authors.lastname: Kosasih
authors.affiliation: U.S. Naval Medical Research Unit No. 2, Jakarta 10560, Indonesia; Health Research Unit, Universitas Padjadjaran, Jalan Eijkman No. 38, Bandung 40161, Indonesia.
authors.forename: Nugroho Harry
authors.initials: NH
authors.lastname: Susanto
authors.affiliation: Clinical Pathology Department, Hasan Sadikin Hospital, Faculty of Medicine, Universitas Padjadjaran, Jalan Pasteur No. 38, Bandung 40161, Indonesia.
authors.forename: Ida
authors.initials: I
authors.lastname: Parwati
authors.affiliation: Health Research Unit, Universitas Padjadjaran, Jalan Eijkman No. 38, Bandung 40161, Indonesia.
authors.forename: Silvita
authors.initials: S
authors.lastname: Fitri
authors.affiliation: Health Research Unit, Universitas Padjadjaran, Jalan Eijkman No. 38, Bandung 40161, Indonesia.
authors.forename: Bachti
authors.initials: B
authors.lastname: Alisjahbana
authors.affiliation: U.S. Naval Medical Research Unit No. 2, Jakarta 10560, Indonesia.
authors.forename: Susana
authors.initials: S
authors.lastname: Widjaja
authors.affiliation: U.S. Naval Medical Research Unit No. 2, Jakarta 10560, Indonesia.
authors.forename: Maya
authors.initials: M
authors.lastname: Williams
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D060085:Coinfection / Q000097:blood* / Q000276:immunology / Q000821:virology; D003715:Dengue / Q000097:blood* / Q000276:immunology / Q000821:virology; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006385:Hemagglutination Inhibition Tests / Q000379:methods*; D006462:Hemolytic Plaque Technique / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests / Q000379:methods
chemical_list: D007074:Immunoglobulin G