title: In Vivo Antitumor Effect of Supercritical CO2Extract of Mango Ginger ( Curcuma amada Roxb) in U-87MG Human Glioblastoma Nude Mice Xenografts.
abstract: Glioblastoma multiforme (GBM) is one the most aggressive and lethal human neoplasms with poor prognosis and very limited positive treatment options. The antitumor effect of supercritical CO2extract of mango ginger ( Curcuma amada Roxb) (CA) with and without irinotecan (IR) was analyzed in U-87MG human glioblastoma multiforme (GBM) cells in vitro and in nude mice xenografts. CA is highly cytotoxic to GBM cells and is synergistic with IR as indicated by the combination index values of <1 in the CompuSyn analysis. CA inhibits tumor growth rate in GBM xenografts, the inhibition rate being higher than in IR treated group. GBM xenograft mice treated with IR + CA combination showed almost complete inhibition of tumor growth rate. Gene expression analysis of xenograft tumors indicated that IR + CA treatment significantly downregulated anti-apoptotic (Bcl-2 and mutant p53), inflammation-associated (COX-2) and cell division-associated (CCNB2) genes and upregulated pro-apoptotic genes (p21 and caspase-3). These results confirmed the therapeutic efficiency of IR + CA combination against GBM and the need for further clinical investigations.
authors.affiliation: 1 Nicklaus Children's Hospital, Miami, FL, USA.|2 Dharma Biomedical LLC, Miami, FL, USA.
authors.forename: Cheppail
authors.initials: C
authors.lastname: Ramachandran
authors.affiliation: 2 Dharma Biomedical LLC, Miami, FL, USA.
authors.forename: Gilda M
authors.initials: GM
authors.lastname: Portalatin
authors.affiliation: 2 Dharma Biomedical LLC, Miami, FL, USA.
authors.forename: Adriana M
authors.initials: AM
authors.lastname: Prado
authors.affiliation: 3 Flavex Naturextrakte GmbH, Rehlingen, Germany.
authors.forename: Karl-Werner
authors.initials: KW
authors.lastname: Quirin
authors.affiliation: 1 Nicklaus Children's Hospital, Miami, FL, USA.
authors.forename: Enrique
authors.initials: E
authors.lastname: Escalon
authors.affiliation: 1 Nicklaus Children's Hospital, Miami, FL, USA.|2 Dharma Biomedical LLC, Miami, FL, USA.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Melnick
keywords: Curcuma amada; gene expression; glioblastoma; mango ginger; therapeutic ratio; xenografts