title: Molecular modification of proanthocyanidins.
abstract: Regioselective enzymatic acylation of proanthocyanidin is proposed and investigated as a method by which to improve the solubility of proanthocyanidins in the oil phase and maintain its oxidation resistance. Experimental results indicate that butanol functions as the best solvent in the studied reaction, in which Lipase Novozym435 is used as biological catalyst enzyme and the molar ratio of lauric acid to proanthocyanidins is 4:1. To increase the esterification conversion, we propose the addition of molecular sieve at 5 h. The product was separated by TLC, and results indicate an optimal solvent ratio of ethyl acetate: petroleum ether: acetic acid = 2:3:0.5. This condition can effectively separate the ester and proanthocyanidins, achieving an esterification yield of 60.9%.
authors.affiliation: a Biochemical Engineering College of Beijing Union University , Beijing , China.|b Beijing; Beijing Key Laboratory of Biomass Waste Resource Utilization , Beijing.
authors.forename: Qing
authors.initials: Q
authors.lastname: Huo
authors.affiliation: a Biochemical Engineering College of Beijing Union University , Beijing , China.
authors.forename: Xiangye
authors.initials: X
authors.lastname: Kong
authors.affiliation: a Biochemical Engineering College of Beijing Union University , Beijing , China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Yang
authors.affiliation: a Biochemical Engineering College of Beijing Union University , Beijing , China.|b Beijing; Beijing Key Laboratory of Biomass Waste Resource Utilization , Beijing.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: c Institute of High Energy Physics, Chinese Academy of Sciences , Beijing , China.|d Beijing Engineering Research Center of Radiographic Techniques and Equipment , Beijing , China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Ma
authors.affiliation: c Institute of High Energy Physics, Chinese Academy of Sciences , Beijing , China.|d Beijing Engineering Research Center of Radiographic Techniques and Equipment , Beijing , China.
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.affiliation: c Institute of High Energy Physics, Chinese Academy of Sciences , Beijing , China.|d Beijing Engineering Research Center of Radiographic Techniques and Equipment , Beijing , China.
authors.forename: Diandou
authors.initials: D
authors.lastname: Xu
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000473:Alkanes / Q000737:chemistry; D000440:Butanols / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000378:metabolism; D004951:Esterification; D006863:Hydrogen-Ion Concentration; D007850:Lauric Acids / Q000737:chemistry; D008049:Lipase / Q000378:metabolism; D044945:Proanthocyanidins / Q000737:chemistry*; D012995:Solubility; D012997:Solvents / Q000737:chemistry
chemical_list: D000473:Alkanes; D000440:Butanols; D004800:Enzymes, Immobilized; D007850:Lauric Acids; D044945:Proanthocyanidins; D012997:Solvents; C030358:lauric acid; D008049:Lipase; C004544:naphtha; D019342:Acetic Acid
keywords: Novozym435; esterification; lipase-catalyzed; peroxide value; proanthocyanidins