title: Ligustrazine attenuates inflammation and the associated chemokines and receptors in ovalbumine-induced mouse asthma model.
abstract: Ligustrazine which is isolated from Chinese herb ligusticum chuanxiong hort, has been widely used in traditional Chinese medicine (TCM) for asthma treatment. In this study, we aim to observe the effect of ligustrazine on inflammation and the associated chemokines and receptors in ovalbumin (OVA)-induced mouse asthma model. Our data demonstrates that ligustrazine suppresses airway hyperresponsiveness to methacholine and lung inflammation in OVA-induced mouse asthma model. Ligustrazine also induces inhibition of inflammatory cells including neutrophils, lymphocytes and eosinophils. In addition, ligustrazine significantly reduces IL-4, IL-5, IL-17A, CCL3, CCL19 and CCL21 level in BALF of asthma mice. Furthermore, ligustrazine induces down-regulation of CCL19 receptor CCR7, STAT3 and p38 MAPK protein expression. Collectively, these results suggest that ligustrazine is effective in attenuation of allergic airway inflammatory changes and related chemokines and receptors in OVA-induced asthma model, and this action might be associated with inhibition of STAT3 and p38 MAPK pathway, which indicates that ligustrazine may be used as a potential therapeutic method to treat asthma.
authors.affiliation: Department of Integrative Medicine, Huashan Hospital, Fudan University, China; The Academy of Integrative Medicine, Fudan University, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Integrative Medicine, Huashan Hospital, Fudan University, China; The Academy of Integrative Medicine, Fudan University, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Integrative Medicine, Huashan Hospital, Fudan University, China; The Academy of Integrative Medicine, Fudan University, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Integrative Medicine, Huashan Hospital, Fudan University, China; The Academy of Integrative Medicine, Fudan University, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Integrative Medicine, Huashan Hospital, Fudan University, China; The Academy of Integrative Medicine, Fudan University, China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Department of Integrative Medicine, Huashan Hospital, Fudan University, China; The Academy of Integrative Medicine, Fudan University, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Integrative Medicine, Huashan Hospital, Fudan University, China; The Academy of Integrative Medicine, Fudan University, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Integrative Medicine, Huashan Hospital, Fudan University, China; The Academy of Integrative Medicine, Fudan University, China.
authors.forename: Mihui
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Integrative Medicine, Huashan Hospital, Fudan University, China; The Academy of Integrative Medicine, Fudan University, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Integrative Medicine, Huashan Hospital, Fudan University, China; The Academy of Integrative Medicine, Fudan University, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wei
authors.affiliation: Department of Integrative Medicine, Huashan Hospital, Fudan University, China; The Academy of Integrative Medicine, Fudan University, China. Electronic address: jcdong2004@126.com.
authors.forename: Jingcheng
authors.initials: J
authors.lastname: Dong
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid; D018925:Chemokines / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D007378:Interleukins / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000473:pathology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology / Q000633:toxicity; D011719:Pyrazines / Q000494:pharmacology*; D054400:Receptors, CCR7 / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism
chemical_list: D018927:Anti-Asthmatic Agents; C515371:Ccr7 protein, mouse; D018925:Chemokines; D007378:Interleukins; D011719:Pyrazines; D054400:Receptors, CCR7; D050796:STAT3 Transcription Factor; D010047:Ovalbumin; C017953:tetramethylpyrazine
keywords: Airway inflammation; Asthma; Chemokines; Ligustrazine; STAT3; p38 MAPK