title: Molecular architecture of the complete COG tethering complex.
abstract: The conserved oligomeric Golgi (COG) complex orchestrates vesicular trafficking to and within the Golgi apparatus. Here, we use negative-stain electron microscopy to elucidate the architecture of the hetero-octameric COG complex from Saccharomyces cerevisiae. Intact COG has an intricate shape, with four (or possibly five) flexible legs, that differs strikingly from that of the exocyst complex and appears to be well suited for vesicle capture and fusion.
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA.
authors.forename: Jun Yong
authors.initials: JY
authors.lastname: Ha
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Hui-Ting
authors.identifier: 0000-0002-8693-8571
authors.initials: HT
authors.lastname: Chou
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ungar
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Calvin K
authors.initials: CK
authors.lastname: Yip
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Walz
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA.
authors.forename: Frederick M
authors.initials: FM
authors.lastname: Hughson
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000648:ultrastructure*; D006056:Golgi Apparatus / Q000648:ultrastructure; D008854:Microscopy, Electron; D046912:Multiprotein Complexes / Q000648:ultrastructure; D020836:Protein Structure, Quaternary; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*; D029701:Saccharomyces cerevisiae Proteins / Q000648:ultrastructure*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D046912:Multiprotein Complexes; D029701:Saccharomyces cerevisiae Proteins