title: Functional and Proteomic Investigations Reveal Major Royal Jelly Protein 1 Associated with Anti-hypertension Activity in Mouse Vascular Smooth Muscle Cells.
abstract: Vascular smooth muscle cells (VSMCs) are a major cell type of the arterial wall and their functionality is associated with blood pressure regulation. Although royal jelly (RJ) has reported effects on anti-hypertension, the mechanism of blood pressure regulation by major royal jelly protein 1 (MRJP1), the most abundant RJ protein, is still unknown. The mrjp1 gene was inserted into mouse VSMCs to investigate how MRJP1 influences VSMC functionality by functional and proteomic analysis. The expression of MRJP1 in VSMCs significantly reduced cell contraction, migration, and proliferation, suggesting a potential role in decreasing hypertension via action on VSMCs. These anti-hypertension activities were further observed in the changes of the proteome setting of mouse VSMCs. Among 675 different proteins after MRJP1 expression, 646 were down-regulated and significantly enriched in pathways implicated in VSMC contraction and migration, which suggest MRJP1 lowers VSMC contraction and migration by inhibiting muscle filament movement. The down-regulated proteins also enriched pathways in proliferation, indicating that MRJP1 hinders VSMC proliferation by reducing the supply of energy and genetic material. This is the first report integrating MRJP1 into VSMC, revealing the function and mechanism correlated with anti-hypertensive activity. This offers a therapeutic potential to control hypertension by gene-therapy using bee-products.
authors.affiliation: Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100093, China.|College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Fan
authors.affiliation: Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Han
authors.affiliation: Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Feng
authors.affiliation: Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fang
authors.affiliation: College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
authors.forename: Han
authors.initials: H
authors.lastname: Hu
authors.affiliation: Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Qi
authors.affiliation: College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
authors.forename: Jianke
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001516:Bees / Q000235:genetics; D001794:Blood Pressure / Q000235:genetics* / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics; D000072669:Gene Editing; D005786:Gene Expression Regulation / Q000235:genetics; D015316:Genetic Therapy; D016678:Genome / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D006973:Hypertension / Q000235:genetics* / Q000503:physiopathology / Q000628:therapy; D019476:Insect Proteins / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D020543:Proteome / Q000235:genetics*
chemical_list: D006023:Glycoproteins; D019476:Insect Proteins; C559157:MRJP1 protein, Apis mellifera; D020543:Proteome