title: Analysis of polymorphism and hepatic expression of duodenal cytochrome b in chronic hepatitis C.
abstract: OBJECTIVE
  Pathological iron overload is commonly found in chronic hepatitis C (CHC) patients and considered as a negative prognostic factor of the disease. A single nucleotide polymorphism (SNP) rs884409 in duodenal cytochrome b gene (CYBRD1) is implicated in the pathogenesis of hemochromatosis. In our study we investigated the impact of the CYBRD1 genotype and expression on iron overload in CHC patients.
  
  
  METHODS
  Liver biopsy specimens and whole blood samples from 243 patients with CHC were included in the study. Iron deposits in hepatocytes, serum markers of iron overload, and expression profile of gene-regulators of iron homeostasis were analyzed. Genotyping and analysis of gene expression of the CYBRD1 were performed. The frequency of SNP and the expression levels of CYBRD1 were compared between the groups of patients with and without markers of iron overload.
  
  
  RESULTS
  The single nucleotide variant rs884409 G was associated with elevated serum iron levels, increased markers of liver inflammation, and oxidative stress. Hepatic expression of CYBRD1 was associated with the expression of Tfr2, Id1, and HO-1 genes, serum ferritin levels, and with increased iron accumulation in liver.
  
  
  CONCLUSIONS
  These results implicate CYBRD1 involvement in iron homeostasis in CHC.
authors.affiliation: Department of Molecular Diagnostics, Intercollegiate Faculty of Biotechnology, University of Gdansk-Medical University of Gdansk, Gdansk, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Rudnicka
authors.affiliation: Department of Molecular Diagnostics, Intercollegiate Faculty of Biotechnology, University of Gdansk-Medical University of Gdansk, Gdansk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Woziwodzka
authors.affiliation: Department of Molecular Diagnostics, Intercollegiate Faculty of Biotechnology, University of Gdansk-Medical University of Gdansk, Gdansk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Wróblewska
authors.affiliation: Department of Molecular Diagnostics, Intercollegiate Faculty of Biotechnology, University of Gdansk-Medical University of Gdansk, Gdansk, Poland.
authors.forename: Magda
authors.initials: M
authors.lastname: Rybicka
authors.affiliation: Department of Molecular Diagnostics, Intercollegiate Faculty of Biotechnology, University of Gdansk-Medical University of Gdansk, Gdansk, Poland.
authors.forename: Krzysztof P
authors.initials: KP
authors.lastname: Bielawski
authors.affiliation: Department of Tropical Medicine and Epidemiology, Medical University of Gdansk, Gdynia, Poland.|Department of Infectious Diseases, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Sikorska
authors.affiliation: Department of Molecular Diagnostics, Intercollegiate Faculty of Biotechnology, University of Gdansk-Medical University of Gdansk, Gdansk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bernat
mesh_terms: D003573:Cytochrome b Group / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000235:genetics; D006432:Hemochromatosis / Q000235:genetics; D019698:Hepatitis C, Chronic / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism*; D019190:Iron Overload / Q000235:genetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide
chemical_list: D003573:Cytochrome b Group; D007501:Iron; D010088:Oxidoreductases; C422926:CYBRD1 protein, human
keywords: CHC; CYBRD1; SNP; iron overload