title: Diagnostic and prognostic value of molecular and serological investigation of human parvovirus B19 infection during pregnancy.
abstract: To define diagnostic and prognostic markers of parvovirus B19 (B19V) fetal infection, two groups were investigated: 1) pregnant women with specific symptoms or contacts with symptomatic households (n=37); 2) mothers with pathological ultrasound findings and the relevant fetus at the time of prenatal diagnosis (n=16). In the first group, diagnosis of B19V infection was achieved using IgM detection in 29/37 (78.3%) of patients, while B19V DNA was detected in 36/37 (97.3%) of infected women. In the second group, intrauterine infection was investigated by amniocentesis (n=5), cordocentesis (n=3) or both (n=5). Median B19V DNA load in amniotic fluid was 8.2x107 copies/ml and in fetal blood was 2x109 copies/ml. Maternal blood was positive for B19V DNA (median 3.8x104 copies/ml) in 14/16 (87.5%) women examined. At time of fetal US investigation, all mothers were B19V IgG positive and B19V IgM were detected in 10/16 (62.5%), while fetal B19V IgG and IgM were detected in 1/8 (12.5%) and 5/8 (62.5%), respectively. Phylogenetic analysis revealed that all B19V maternal and fetal strains belonged to genotype 1A. Diagnosis of maternal, fetal and neonatal B19V infection should be based on both IgM and DNA detection. Prognostic markers of congenital B19V infection need to be defined.
authors.affiliation: Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Zavattoni
authors.affiliation: Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Paolucci
authors.affiliation: Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Sarasini
authors.affiliation: Obstetric and Gynecology Unit, Fondazione IRCCS Ospedale Maggiore Policlinico di Milano, Italy.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Tassis
authors.affiliation: Departments of Obstetrics and Gynecology, "V.Buzzi" Hospital, ICP, University of Milan, Italy.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Rustico
authors.affiliation: Departments of Obstetrics and Gynecology, "V.Buzzi" Hospital, ICP, University of Milan, Italy.
authors.forename: Aida
authors.initials: A
authors.lastname: Quarenghi
authors.affiliation: Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Piralla
authors.affiliation: Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.|Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of.
authors.forename: Fausto
authors.initials: F
authors.lastname: Baldanti
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D004279:DNA, Viral / Q000097:blood; D016731:Erythema Infectiosum / Q000097:blood* / Q000473:pathology / Q000821:virology; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D016732:Parvovirus B19, Human; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000097:blood / Q000175:diagnosis / Q000821:virology*; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral
keywords: Fetal and neonatal diagnosis; Maternal; Parvovirus B19