title: Long noncoding ribonucleic acids maternally expressed gene 3 inhibits lung cancer tumor progression through downregulation of MYC.
abstract: OBJECTIVE
  Long noncoding ribonucleic acids (RNAs) nowadays emerge as important biomarkers or potential therapeutic targets discussed in human cancers. Among them, maternally expressed gene 3 (MEG3) is known to be decreased in a variety of malignancies.
  
  
  METHODS
  Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed to detect the expression of MEG3 in forty pairs of lung cancer (LC) tissues. Overexpression of MEG3 was carried out, and we determined its effect on cell proliferation, apoptosis, and migration evaluated by cell counting kit-8, flow cytometric, and transwell analysis. Messenger RNA and protein expression of MYC were determined by qRT-PCR and western blot, respectively.
  
  
  RESULTS
  The expression of MEG3 was downregulated in LC tissues. Forced expression of MEG3 led to reduced abilities of cell proliferation and elevated apoptosis rate. It also slightly inhibited cell migration capacity in vitro. In addition, MYC was inhibited by MEG3 overexpression at both transcriptional and translational levels.
  
  
  CONCLUSIONS
  Our findings revealed MEG3 could regulate LC progression and serve as an important target for LC treatment.
authors.affiliation: Department of Oncology, Huaihe Hospital of Henan University, Kaifeng, China.
authors.forename: L
authors.initials: L
authors.lastname: Yan-Hua
authors.affiliation: Department of Oncology, Huaihe Hospital of Henan University, Kaifeng, China.
authors.forename: L
authors.initials: L
authors.lastname: Xiang-Lei
authors.affiliation: Department of Oncology, Huaihe Hospital of Henan University, Kaifeng, China.
authors.forename: L
authors.initials: L
authors.lastname: Hong
authors.affiliation: Department of Oncology, Huaihe Hospital of Henan University, Kaifeng, China.
authors.forename: W
authors.initials: W
authors.lastname: Jian-Jun
mesh_terms: D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D015536:Down-Regulation; D016259:Genes, myc; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D062085:RNA, Long Noncoding / Q000235:genetics*; D014162:Transfection
chemical_list: C498907:MEG3 non-coding RNA, human; D062085:RNA, Long Noncoding