title: Characterizing a novel and sensitive method to measure dsRNA in soil.
abstract: Performing environmental assessments for double-stranded RNA-based agricultural products require the development of sensitive and selective methods to measure biodegradation rates of dsRNAs. We developed and characterized a novel analytical procedure that uses a molecular hybridization assay (QuantiGene(®)) to accurately measure dsRNA extracted from diverse soils. In this report, we utilize this method to demonstrate that two dsRNAs with distinct size, structure, and sequence degrade rapidly in soil with indistinguishable kinetics.
authors.affiliation: Regulatory Sciences, Monsanto Company, 800 N. Lindbergh Blvd., Saint Louis, MO 63167, USA. Electronic address: joshua.r.fischer@monsanto.com.
authors.forename: Joshua R
authors.initials: JR
authors.lastname: Fischer
authors.affiliation: Regulatory Sciences, Monsanto Company, 800 N. Lindbergh Blvd., Saint Louis, MO 63167, USA.
authors.forename: Fatima
authors.initials: F
authors.lastname: Zapata
authors.affiliation: Regulatory Sciences, Monsanto Company, 800 N. Lindbergh Blvd., Saint Louis, MO 63167, USA.
authors.forename: Samuel
authors.initials: S
authors.lastname: Dubelman
authors.affiliation: Regulatory Sciences, Monsanto Company, 800 N. Lindbergh Blvd., Saint Louis, MO 63167, USA.
authors.forename: Geoffrey M
authors.initials: GM
authors.lastname: Mueller
authors.affiliation: Regulatory Sciences, Monsanto Company, 800 N. Lindbergh Blvd., Saint Louis, MO 63167, USA.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Jensen
authors.affiliation: Regulatory Sciences, Monsanto Company, 800 N. Lindbergh Blvd., Saint Louis, MO 63167, USA.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Levine
mesh_terms: D000383:Agriculture; D004784:Environmental Monitoring / Q000379:methods*; D019476:Insect Proteins / Q000235:genetics; D057230:Limit of Detection; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified / Q000235:genetics; D012330:RNA, Double-Stranded / Q000032:analysis*; D018749:RNA, Plant / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D019476:Insect Proteins; D012330:RNA, Double-Stranded; D018749:RNA, Plant; D012987:Soil
keywords: DvSnf7; Environmental fate; QuantiGene; Soil; dsRNA