title: Universal Parameter Optimization of Density Gradient Ultracentrifugation Using CdSe Nanoparticles as Tracing Agents.
abstract: Density gradient ultracentrifugation (DGUC) has recently emerged as an effective nanoseparation method to sort polydispersed colloidal NPs mainly according to their size differences to reach monodispersed fractions (NPs), but its separation modeling is still lack and the separation parameters' optimization mainly based on experience of operators. In this paper, we gave mathematical descriptions on the DGUC separation, which suggested the best separation parameters for a given system. The separation parameters, including media density, centrifuge speed and time, which affected the separation efficiency, were discussed in details. Further mathematical optimization model was established to calculate and yield the "best" (optimized) linear gradient for a colloidal system with given size and density. The practical experiment results matched well with theoretical prediction, demonstrating the DGUC method, an efficient, practical, and predictable separation technique with universal utilization for colloid sorting.
authors.affiliation: State Key Lab of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Pengsong
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Lab of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Jinyang
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Lab of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Luo
authors.affiliation: State Key Lab of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Kuang
authors.affiliation: State Key Lab of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Sun