title: Simultaneous determination of polycyclic aromatic hydrocarbons and tobacco-specific N-nitrosamines in mainstream cigarette smoke using in-pipette-tip solid-phase extraction and on-line gel permeation chromatography-gas chromatography-tandem mass spectrometry.
abstract: In this study, a silica/primary secondary amine (SiO2/PSA) was used as an in-pipette-tip solid phase extraction (SPE) sorbent for the simultaneous determination of polycyclic aromatic hydrocarbons (PAHs) and tobacco-specific N-nitrosamines (TSNAs) in mainstream cigarette smoke (MSS). We investigated several parameters including an extraction procedure of total particulate matter, type and amount of sorbent and on-line gel permeation chromatography parameters to obtain optimum conditions for a new strategy to target analytes. Under the optimized conditions, we developed a method for the simultaneous determination of PAHs and TSNAs in MSS by coupling in-pipette-tip SPE procedures to an on-line gel permeation chromatography-gas chromatography-tandem mass spectrometry (on-line GPC-GC-MS(2)). Our method had limits of detection for target analytes ranging from 0.01 to 0.23ng/cig. Good linearities were obtained with coefficients of determination (R(2)) greater than 0.9984 for all target analytes. Good reproducibility was obtained as intra- and inter-day precisions, and the relative standard deviations were less than 11.4 and 13.3%, respectively. The recoveries were in the range of 77.1-108.6% at different concentrations for real samples. Compared to previous standard methods for the determination of PAHs and TSNAs in MSS, our method was highly effective, fast, and had low consumption of organic solvent and a high degree of automation. Finally, our method successfully analyzed PAHs and TSNAs in real samples, and no significant deviations were observed when compared to similar analysis using standard methods.
authors.affiliation: China National Tobacco Quality Supervision and Test Center, No. 2 Fengyang Street, Zhengzhou High and New Technology Industries Development Zone, Zhengzhou 450001, China.
authors.forename: Yan-Bo
authors.initials: YB
authors.lastname: Luo
authors.affiliation: China National Tobacco Quality Supervision and Test Center, No. 2 Fengyang Street, Zhengzhou High and New Technology Industries Development Zone, Zhengzhou 450001, China.
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Chen
authors.affiliation: China National Tobacco Quality Supervision and Test Center, No. 2 Fengyang Street, Zhengzhou High and New Technology Industries Development Zone, Zhengzhou 450001, China.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Zhang
authors.affiliation: China National Tobacco Quality Supervision and Test Center, No. 2 Fengyang Street, Zhengzhou High and New Technology Industries Development Zone, Zhengzhou 450001, China.
authors.forename: Xing-Yi
authors.initials: XY
authors.lastname: Jiang
authors.affiliation: China National Tobacco Quality Supervision and Test Center, No. 2 Fengyang Street, Zhengzhou High and New Technology Industries Development Zone, Zhengzhou 450001, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: China National Tobacco Quality Supervision and Test Center, No. 2 Fengyang Street, Zhengzhou High and New Technology Industries Development Zone, Zhengzhou 450001, China.
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Li
authors.affiliation: China National Tobacco Quality Supervision and Test Center, No. 2 Fengyang Street, Zhengzhou High and New Technology Industries Development Zone, Zhengzhou 450001, China.
authors.forename: Feng-Peng
authors.initials: FP
authors.lastname: Zhu
authors.affiliation: China National Tobacco Quality Supervision and Test Center, No. 2 Fengyang Street, Zhengzhou High and New Technology Industries Development Zone, Zhengzhou 450001, China. Electronic address: 36140501@qq.com.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Pang
authors.affiliation: China National Tobacco Quality Supervision and Test Center, No. 2 Fengyang Street, Zhengzhou High and New Technology Industries Development Zone, Zhengzhou 450001, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Hou
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards; D002850:Chromatography, Gel; D008401:Gas Chromatography-Mass Spectrometry; D057230:Limit of Detection; D009602:Nitrosamines / Q000032:analysis*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D012906:Smoke / Q000032:analysis*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D009602:Nitrosamines; D011084:Polycyclic Aromatic Hydrocarbons; D012906:Smoke; D012822:Silicon Dioxide
keywords: In-pipette-tip solid phase extraction (SPE); On-line gel permeation chromatography-gas chromatography/tandem mass spectrometry (on-line GPC-GC–MS(2)); Polycyclic aromatic hydrocarbons (PAHs); Silica/primary secondary amine (SiO(2)/PSA); Tobacco-specific N-nitrosamines (TSNAs)