title: Significance of immune response to Mycobacterium tuberculosis culture filtrate protein antigens in cerebrospinal fluid of tuberculous meningitis patients: A search for diagnostic marker.
abstract: Mycobacterium tuberculosis (H37Ra) culture filtrate proteins (CFP) are explored as a diagnostic marker for tuberculous meningitis (TBM). Cerebrospinal fluid (CSF) samples from patients were categorized as confirmed (n = 47), suspected (n = 20), and non-TBM (n = 25) cases. Immune response by Western blot revealed TBM CSF samples are having heterogeneous response to CFP. CFP ELISA was 92% sensitive and 38.30% specific. ODs of confirmed TBM and non-TBM cases were significantly different (P < 0.0001) and also the suspected TBM and non-TBM cases (P = 0.0001). No significant difference noticed in TBM and suspected TBM (P = 0.90). Thus, CFP can be a better biomarker for the diagnosis of TBM.
authors.affiliation: a Department of Neuromicrobiology , National Institute of Mental Health and Neurosciences (NIMHANS) , Bangalore , Karnataka , India.
authors.forename: Prashant
authors.initials: P
authors.lastname: Giribhattanavar
authors.affiliation: a Department of Neuromicrobiology , National Institute of Mental Health and Neurosciences (NIMHANS) , Bangalore , Karnataka , India.
authors.forename: Kavitha
authors.initials: K
authors.lastname: Kumar
authors.affiliation: b Medical Biotechnology Division , School of Biosciences and Technology, VIT University , Vellore , India.
authors.forename: Siddarth
authors.initials: S
authors.lastname: Raajasekar
authors.affiliation: a Department of Neuromicrobiology , National Institute of Mental Health and Neurosciences (NIMHANS) , Bangalore , Karnataka , India.
authors.forename: Nagarathna
authors.initials: N
authors.lastname: Chandrashekar
authors.affiliation: a Department of Neuromicrobiology , National Institute of Mental Health and Neurosciences (NIMHANS) , Bangalore , Karnataka , India.
authors.forename: Shripad A
authors.initials: SA
authors.lastname: Patil
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology*; D002555:Cerebrospinal Fluid / Q000276:immunology* / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000276:immunology*; D014390:Tuberculosis, Meningeal / Q000134:cerebrospinal fluid* / Q000175:diagnosis* / Q000382:microbiology; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial
keywords: ELISA; Western blot; diagnosis; heterogeneous; meningitis