title: miR-1303 promotes the proliferation of neuroblastoma cell SH-SY5Y by targeting GSK3β and SFRP1.
abstract: Neuroblastoma (NB) is one of the most common solid tumors in children, many microRNAs regulate progression and development of NB. Here, we found miR-1303 was upregulated in NB cells and tissues, miR-1303 overexpression promoted the proliferation of SH-SY5Y NB cell investigated by MTT assay, colony formation assay and anchorage-independent growth ability assay, while miR-1303 knockdown reduced this effect. mechanism analysis suggested glycogen synthase kinase 3 beta (GSK3β) and secreted frizzled-related protein 1 (SFRP1) were the target of miR-1303, luciferase assay revealed miR-1303 directly bound to the 3'UTR of GSK3β and SFRP1. miR-1303 increased expression of MYC and CyclinD1, and decreased the expression of p21 and p27, and further demonstrated miR-1303 promotes NB proliferation. Moreover, there was a negative correlation between miR-1303 expression and GSK3β and SFRP1 expression in NB tissues, confirming GSK3β and SFRP1 were the targets of miR-1303 in NB tissues. Collectively, our findings suggested miR-1303 promotes NB proliferation by targeting GSK3β and SFRP1, and might be a target for NB therapy.
authors.affiliation: Department of Pediatric Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China, China.
authors.forename: Zuoqing
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Pediatric Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Pediatric Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China, China.
authors.forename: Qigen
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Department of Pediatric Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China, China.
authors.forename: Wenzong
authors.initials: W
authors.lastname: Gao
authors.affiliation: Department of Pediatric Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China, China.
authors.forename: Juntao
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Pediatric Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China, China. Electronic address: lizhou18@hotmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D000071679:Glycogen Synthase Kinase 3 beta / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics; D000099274:Frizzled-Related Proteins
chemical_list: D000071679:Glycogen Synthase Kinase 3 beta; D047908:Intracellular Signaling Peptides and Proteins; D035683:MicroRNAs; D011506:Proteins; D000099274:Frizzled-Related Proteins; C000592164:MIRN1303 microRNA, human
keywords: GSK3β; Neuroblastoma; Proliferation; SFRP1; miR-1303