title: Extensive characterization of feline intra-abdominal adipose-derived mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) isolated from various tissues have been well characterized for therapeutic application to clinical diseases. However, in contrast to MSCs from other animal species, the characteristics of feline MSCs have not been fully documented. In this study, we conducted extensive characterization of feline adipose tissue-derived MSCs (fAD-MSCs). Study fAD-MSCs were individually isolated from the intra-abdominal adipose tissues of six felines. The expression levels of cell surface markers and pluripotent markers were evaluated. Next, proliferation capacity was analyzed by performing cumulative population doubling level (CPDL) and doubling time (DT) calculation assays. Differentiation potentials of fAD-MSCs into mesodermal cell lineages were analyzed by examining specific staining and molecular markers. All fAD-MSCs positively expressed cell surface markers such as CD29, CD44, CD90, CD105, CD166, and MHC-I, while CD14, CD34, CD45, and CD73 were negatively expressed. The CPDL of the fAD-MSCs was maintained until passage 5 to 6 (P5 to P6), whereas DT increased after P3 to P4. Also, stem cell-specific pluripotent markers (Oct3/4, Nanog, and SSEA-4) were detected. Importantly, all fAD-MSCs demonstrated mesodermal differentiation capacity. These results suggest that fully characterized fAD-MSCs could be beneficial when considering the use of these cells in feline disease research.
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: Hee-Ryang
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: Jienny
authors.initials: J
authors.lastname: Lee
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: Jeong Su
authors.initials: JS
authors.lastname: Byeon
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: Na-Yeon
authors.initials: NY
authors.lastname: Gu
authors.affiliation: Kangnam Animal Hospital, Pyeongtaek 17982, Korea.
authors.forename: Jiyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Cho
authors.affiliation: Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Cha
mesh_terms: D000005:Abdomen; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002415:Cats / Q000033:anatomy & histology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D005434:Flow Cytometry / Q000662:veterinary; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D039902:Multipotent Stem Cells / Q000166:cytology / Q000502:physiology
chemical_list: D015415:Biomarkers
keywords: adipose tissue-derived mesenchymal stem cells; differentiation; feline; multipotent; proliferation