title: Stereological Quantification of Cell-Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing.
abstract: We describe a stereology method to obtain reliable estimates of the total number of proliferative and migratory epithelial cells after wounding. Using pulse and chase experiments with halogenated thymidine analogs such as iododeoxyuridine (IdU) and chlorodeoxyuridine (CldU), it is possible to track epithelial populations with heterogeneous proliferative characteristics through skin compartments. The stereological and tissue processing methods described here apply widely to address important questions of skin stem-cell biology.
authors.affiliation: Laboratory of RNA & Extracellular Vesicles, Instituto Nacional de Medicina Genómica, Periférico Sur 4809, Tlalpan, Ciudad de México, 14610, México. emartinez@inmegen.gob.mx.|Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 741, Boston, MA, 02115, USA. emartinez@inmegen.gob.mx.|Departamento de Biología Celular y Fisiología and Grupo Multidisciplinario de Investigación en Células Troncales IMPULSA 02, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Coyoacán, Ciudad de México, 04510, Mexico. emartinez@inmegen.gob.mx.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Martínez-Martínez
authors.affiliation: División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma deMéxico, Coyoacán, Ciudad de México, 04510, Mexico.
authors.forename: Eileen
authors.initials: E
authors.lastname: Uribe-Querol
authors.affiliation: Departamento de Biología Celular y Fisiología and Grupo Multidisciplinario de Investigación en Células Troncales IMPULSA 02, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Coyoacán, Ciudad de México, 04510, Mexico.
authors.forename: Claudio I
authors.initials: CI
authors.lastname: Galván-Hernández
authors.affiliation: Departamento de Biología Celular y Fisiología and Grupo Multidisciplinario de Investigación en Células Troncales IMPULSA 02, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Coyoacán, Ciudad de México, 04510, Mexico.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Gutiérrez-Ospina
mesh_terms: D002453:Cell Cycle; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D014945:Wound Healing
keywords: Bromodeoxyuridine; Bulge; Chlorodeoxyuridine; Confocal microscopy; Epidermis; Hair follicles; Halogenated thymidine analogs; Iododeoxyuridine; Keratinocytes; Nucleoside; Random sampling; Stem cells; Stereology; Wound