title: Enhanced Signal and Quantitative Detection of Anti-Interferon-Gamma Antibody by Using a Nanometer Biolinker.
abstract: For rapid screening and quantification of an antisera antibody, a nanometer bithiophene-based conductive biolinker can enhanced signal performance and can be used to verify the interaction of an anti-IFN-γ antibody with an IFN-γ protein. The experimental measurements take a generic approach which takes advantage of the functionality of thiophene-based linkers for biosensors. Effects associated with using bithiophene as a biolinker for surface plasmon resonance (SPR) spectroscopy are examined in this paper. By using an atomic force microscope (AFM), it was observed that the morphology of the bithiophene modified gold sensor surface became smoother than the original gold surface. We compared the response and concentration of the anti-IFN-γ antibody on a bithiophene-coated and dextran-coated biochip as well as on different thickness-modified surfaces under SPR relevant conditions. The results indicate that a response to IFN-γ molecules immobilized on a sensor using a bithiophene biolinker improved more than 8-fold when compared to that of a sensor using a dextran biolinker. Furthermore, the regeneration ability of the sensor surface shows good repeatability as only less than a 1% decrease was found after repeating the experimental work over 6 cycles. The characteristics provided us with a good platform for rapid screening, real-time monitoring and quantitative concentration of the autoimmune antibody activities.
authors.affiliation: Department of Engineering Science & Ocean Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Pei-I
authors.initials: PI
authors.lastname: Tsai
authors.affiliation: Department of Chemistry, Tamkang University, New Taipei City, Taiwan.
authors.forename: Adam Shih-Yuan
authors.initials: AS
authors.lastname: Lee
authors.affiliation: Department of Systems Engineering & Naval Architecture, National Taiwan Ocean University, Keelung, Taiwan.
authors.forename: Shu-Sheng
authors.initials: SS
authors.lastname: Lee
authors.affiliation: Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan.
authors.forename: Ming-Han
authors.initials: MH
authors.lastname: Chung
authors.affiliation: Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan.
authors.forename: Meng-Wei
authors.initials: MW
authors.lastname: Liu
authors.affiliation: Department of Engineering Science & Ocean Engineering, National Taiwan University, Taipei, Taiwan.|Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan.
authors.forename: Chih-Kung
authors.initials: CK
authors.lastname: Lee
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology*; D015374:Biosensing Techniques / Q000379:methods*; D003911:Dextrans / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology*; D051379:Mice; D036103:Nanotechnology / Q000379:methods*; D012680:Sensitivity and Specificity; D013876:Thiophenes / Q000737:chemistry
chemical_list: D000906:Antibodies; D003911:Dextrans; D055767:Immobilized Proteins; D013876:Thiophenes; D006046:Gold; D007371:Interferon-gamma