title: MicroRNA Expression in a Readily Accessible Common Hepatic Artery Lymph Node Predicts Time to Pancreatic Cancer Recurrence Postresection.
abstract: Lymph node involvement in pancreatic adenocarcinoma (PAC) predicts postresection survival, but early lymph node metastasis detection is not easily accomplished. We assessed a panel of microRNAs (miRNAs) in a common hepatic artery lymph node (station 8) that is readily accessible during pancreatoduodenectomy (PD) to determine if increased miRNA levels correlate with postresection recurrence. Station 8 lymph nodes overlying the common hepatic artery collected during PD were assayed for miRNA-10b, miRNA-30c, miRNA-21, and miRNA-155 and cytokeratin-19 (CK19), an epithelial cell marker, using quantitative PCR. Expression was correlated with disease recurrence, recurrence-free survival (RFS), and overall survival (OS). Station 8 lymph nodes from 37 patients (30 periampullary carcinomas (PCs), 2 chronic pancreatitis, 5 other cancers) exhibited increased miRNA-10b levels in 14/30 PCs, and in 10 of these 14 patients, cancer recurred during the study period (2012-2015). High miRNA-10b was also associated with shorter RFS (42.5 vs. 92.4 weeks, p < 0.05) but not OS, whereas miRNA-30c, miRNA-21, and miRNA-155 levels and CK19 mRNA levels in station 8 nodes were variable and did not correlate with RFS or OS. We conclude that elevated miRNA-10b levels in station 8 lymph nodes could be utilized to assess risk for early disease progression in patients with periampullary tumors.
authors.affiliation: Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
authors.forename: Hai V
authors.initials: HV
authors.lastname: Nguyen
authors.affiliation: Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.|The Pancreatic Cancer Signature Center, Indiana University Simon Cancer Center, Indianapolis, IN, 46202, USA.
authors.forename: Jesse
authors.initials: J
authors.lastname: Gore
authors.affiliation: Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhong
authors.affiliation: Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
authors.forename: Sudha S
authors.initials: SS
authors.lastname: Savant
authors.affiliation: Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
authors.forename: Samantha
authors.initials: S
authors.lastname: Deitz-McElyea
authors.affiliation: Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.|The Pancreatic Cancer Signature Center, Indiana University Simon Cancer Center, Indianapolis, IN, 46202, USA.
authors.forename: C Max
authors.initials: CM
authors.lastname: Schmidt
authors.affiliation: Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.|The Pancreatic Cancer Signature Center, Indiana University Simon Cancer Center, Indianapolis, IN, 46202, USA.
authors.forename: Michael G
authors.initials: MG
authors.lastname: House
authors.affiliation: Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, 46202, USA. mkorc@iu.edu.|The Pancreatic Cancer Signature Center, Indiana University Simon Cancer Center, Indianapolis, IN, 46202, USA. mkorc@iu.edu.|Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, 46202, USA. mkorc@iu.edu.|IU Simon Cancer Center, Indiana University School of Medicine, 980 West Walnut Street, Rm 528, Indianapolis, IN, 46202, USA. mkorc@iu.edu.
authors.forename: Murray
authors.identifier: 0000-0003-1566-0899
authors.initials: M
authors.lastname: Korc
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology / Q000601:surgery*; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006499:Hepatic Artery; D006801:Humans; D053539:Keratin-19 / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism* / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000473:pathology / Q000601:surgery*; D016577:Pancreaticoduodenectomy
chemical_list: D053539:Keratin-19; D035683:MicroRNAs
keywords: Lymph node; MicroRNA; Pancreatic cancer