title: Dynamic Modulation of DNA Hybridization Using Allosteric DNA Tetrahedral Nanostructures.
abstract: The fixed dynamic range of traditional biosensors limits their utility in several real applications. For example, viral load monitoring requires the dynamic range spans several orders of magnitude; whereas, monitoring of drugs requires extremely narrow dynamic range. To overcome this limitation, here, we devised tunable biosensing interface using allosteric DNA tetrahedral bioprobes to tune the dynamic range of DNA biosensors. Our strategy takes the advantage of the readily and flexible structure design and predictable geometric reconfiguration of DNA nanotechnology. We reconfigured the DNA tetrahedral bioprobes by inserting the effector sequence into the DNA tetrahedron, through which, the binding affinity of DNA tetrahedral bioprobes can be tuned. As a result, the detection limit of DNA biosensors can be programmably regulated. The dynamic range of DNA biosensors can be tuned (narrowed or extended) for up to 100-fold. Using the regulation of binding affinity, we realized the capture and release of biomolecules by tuning the binding behavior of DNA tetrahedral bioprobes.
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Song
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University , Shanghai 200241, China.
authors.forename: Juwen
authors.initials: J
authors.lastname: Shen
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University , Shanghai 200241, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Pei
authors.affiliation: Key Laboratory for Organic Electronics and Information Displays (KLOEID), Institute of Advanced Materials (IAM) and School of Materials Science and Engineering, Nanjing University of Posts and Telecommunications , Nanjing, 210046, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chao
authors.affiliation: Key Laboratory for Organic Electronics and Information Displays (KLOEID), Institute of Advanced Materials (IAM) and School of Materials Science and Engineering, Nanjing University of Posts and Telecommunications , Nanjing, 210046, China.
authors.forename: Shao
authors.initials: S
authors.lastname: Su
authors.affiliation: Chemistry Department, King Saud University , Riyadh 11451, Saudi Arabia.
authors.forename: Ali
authors.initials: A
authors.lastname: Aldalbahi
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.affiliation: Kellogg College, University of Oxford , Oxford, OX2 6PN, United Kingdom.
authors.forename: Jiye
authors.initials: J
authors.lastname: Shi
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
authors.affiliation: Key Laboratory for Organic Electronics and Information Displays (KLOEID), Institute of Advanced Materials (IAM) and School of Materials Science and Engineering, Nanjing University of Posts and Telecommunications , Nanjing, 210046, China.
authors.forename: Lianhui
authors.initials: L
authors.lastname: Wang
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
mesh_terms: D000494:Allosteric Regulation; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry* / Q000378:metabolism; D055664:Electrochemical Techniques; D004566:Electrodes; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D004247:DNA