title: Inhibitor effect of paricalcitol in rat model of pentylenetetrazol-induced seizures.
abstract: Vitamin D has various systemic effects on bone metabolism, modulation of the immune system, stabilization of the cell membrane, oxidative stress, inflammation, apoptosis, and various other hormones. Differing from active vitamin D, paricalcitol is a relatively safe VDR agonist due to its relatively few side effects. This study has investigated the anticonvulsant effect of paricalcitol in convulsions induced by pentylenetetrazole (PTZ). 36 male Sprague-Dawley rats were divided randomly into two groups: 18 for EEG recording (PTZ 35 mg/kg) and 18 for behavioral studies (PTZ 70 mg/kg). Forty-five minutes before the PTZ injection, both groups of rats were given 5 and 10 μg/kg of paricalcitol i.p., respectively. Racine convulsion scores, first myoclonic jerk time, spike percentages, and antioxidant status were evaluated in the groups. Our results showed that the Racine's Convulsion Scale (RCS) score significantly dropped in the paricalcitol-treated group, analysis of the first myoclonic jerk (FMJ) latencies demonstrated a significantly longer latency in the paricalcitol-applied group, and spike percentages at EEG recordings significantly decreased with paricalcitol. Moreover, MDA levels were lower and SOD activity were higher in the 5 μg/kg paricalcitol group compared to the saline group; these results were more prominent in 10 μg/kg paricalcitol group. Our study has demonstrated that paricalcitol has protective effects on PTZ-induced convulsions. Based on the SOD and MDA levels in our study, these effects may result from the antioxidant characteristics of paricalcitol.
authors.affiliation: Department of Histology and Embryology, Ege University School of Medicine, Izmir, Turkey. yigit.uyanikgil@ege.edu.tr.|Cord Blood, Cell-Tissue Application and Research Center, Ege University, Izmir, Turkey. yigit.uyanikgil@ege.edu.tr.
authors.forename: Yiğit
authors.initials: Y
authors.lastname: Uyanıkgil
authors.affiliation: Department of Neurology, Trakya University Medical Faculty, Edirne, Turkey.
authors.forename: Volkan
authors.initials: V
authors.lastname: Solmaz
authors.affiliation: Department of Histology and Embryology, Ege University School of Medicine, Izmir, Turkey.|Cord Blood, Cell-Tissue Application and Research Center, Ege University, Izmir, Turkey.
authors.forename: Türker
authors.initials: T
authors.lastname: Çavuşoğlu
authors.affiliation: Department of Neurology, Samsun Training and Research Hospital, Samsun, Turkey.
authors.forename: Bilge Piri
authors.initials: BP
authors.lastname: Çınar
authors.affiliation: Department of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey.
authors.forename: Emel Öykü
authors.initials: EÖ
authors.lastname: Çetin
authors.affiliation: Department of Internal Medicine, Division of Pathophysiology, Ankara University Faculty of Medicine, Ankara, Turkey.
authors.forename: Halil Yılmaz
authors.initials: HY
authors.lastname: Sur
authors.affiliation: Department of Physiology, Bilim University School of Medicine, Istanbul, Turkey.
authors.forename: Oytun
authors.initials: O
authors.lastname: Erbaş
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D058256:Brain Waves / Q000187:drug effects*; D004195:Disease Models, Animal; D004569:Electroencephalography; D004872:Ergocalciferols / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010433:Pentylenetetrazole; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control* / Q000523:psychology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000927:Anticonvulsants; D000975:Antioxidants; D015415:Biomarkers; D004872:Ergocalciferols; D008315:Malondialdehyde; C084656:paricalcitol; D013482:Superoxide Dismutase; D010433:Pentylenetetrazole
keywords: Electroencephalogram; Experimental epilepsy model; Paricalcitol; Pentylenetetrazol