title: miR-10b Inhibits Apoptosis and Promotes Proliferation and Invasion of Endometrial Cancer Cells via Targeting HOXB3.
abstract: MicroRNAs are small RNA that are tightly interrelated with the initiation, development, and metastasis of cancers. Studies have shown that miR-10b is increased in various cancers. However, the underlying mechanisms of miR-10b in the occurrence and metastasis of endometrial cancer are poorly understood. To investigate its roles and correlations with Homeobox box 3 (HOXB3) in endometrial cancer, cancer tissues and adjacent normal endometrium tissues from 20 patients with endometrial cancer were studied. miR-10b expression was significantly up-regulated (p < 0.01) in endometrial cancer tissue, whereas HOXB3 was lowly expressed. The silence of miR-10b resulted in significantly enhanced cell apoptosis, and remarkably reduced cell proliferation, migration, and invasion (p < 0.05). Moreover, the protein levels of HOXB3 were increased in KLE cells with silenced miR-10b, and dual-luciferase reporter assay suggested that miR-10b could directly target HOXB3. Furthermore, overexpression of HOXB3 promoted cell apoptosis but inhibited cell proliferation, migration, and invasion (p < 0.01). To conclude, miR-10b might control cell apoptosis, proliferation, migration, and invasion in endometrial cancer via regulation of HOXB3 expression.
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of GuangXi Medical University , Nanning, Guangxi Province, The People's Republic of China .
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of GuangXi Medical University , Nanning, Guangxi Province, The People's Republic of China .
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of GuangXi Medical University , Nanning, Guangxi Province, The People's Republic of China .
authors.forename: Wensheng
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of GuangXi Medical University , Nanning, Guangxi Province, The People's Republic of China .
authors.forename: Junying
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of GuangXi Medical University , Nanning, Guangxi Province, The People's Republic of China .
authors.forename: Chaohuan
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of GuangXi Medical University , Nanning, Guangxi Province, The People's Republic of China .
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of GuangXi Medical University , Nanning, Guangxi Province, The People's Republic of China .
authors.forename: Di
authors.initials: D
authors.lastname: Fang
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of GuangXi Medical University , Nanning, Guangxi Province, The People's Republic of China .
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D005260:Female; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D014162:Transfection
chemical_list: D018398:Homeodomain Proteins; C542921:HoxB3 protein, human; C547183:MIRN10 microRNA, human; D035683:MicroRNAs
keywords: HOXB3; apoptosis; endometrial cancer; invasion; miR-10b; proliferation