title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal.
abstract: Prunus persica fruits were removed from 1-year-old shoots to analysis photosynthesis, chlorophyll fluorescence and genes changes in leaves to low sink demand caused by fruit removal (-fruit) during the final stage of rapid fruit growth. A decline in net photosynthesis rate was observed, accompanied with a decrease in stomatal conductance. The intercellular CO2 concentrations and leaf temperature increased as compared with a normal fruit load (+fruit). Moreover, low sink demand significantly inhibited the donor side and the reaction center of photosystem II. 382 genes in leaf with an absolute fold change ≥1 change in expression level, representing 116 up- and 266 down-regulated genes except for unknown transcripts. Among these, 25 genes for photosynthesis were down-regulated, 69 stress and 19 redox related genes up-regulated under the low sink demand. These studies revealed high leaf temperature may result in a decline of net photosynthesis rate through down-regulation in photosynthetic related genes and up-regulation in redox and stress related genes, especially heat shock proteins genes. The complex changes in genes at the transcriptional level under low sink demand provided useful starting points for in-depth analyses of source-sink relationship in P. persica.
authors.affiliation: Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Duan
authors.affiliation: Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences Beijing, China.
authors.forename: Hongguo
authors.initials: H
authors.lastname: Xu
authors.affiliation: Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences Beijing, China.
authors.forename: Guotian
authors.initials: G
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences Beijing, China.
authors.forename: Peige
authors.initials: P
authors.lastname: Fan
authors.affiliation: Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences Beijing, China.
authors.forename: Zhenchang
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences Beijing, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Li
keywords: fruit removal; low sink demand; peach; photosynthesis; transcriptional profile