title: Peripheral blood stem cells collection on spectra optia apheresis system using the continuous mononuclear cell collection protocol: A single center report of 39 procedures.
abstract: BACKGROUND
  The aim of the study was to assess the performance of the new Continuous Mononuclear Cell Collection (CMNC) protocol on the Spectra Optia Apheresis System for collecting autologous Peripheral Blood Stem Cells (PBSC) in adult patients with respect to collection variables, CD34+ cells harvest prediction and engraftment data. In this retrospective study, 39 CMNC procedures on 23 mobilized patients with multiple myeloma and lymphoma were analyzed. CD34+ cells and blood cells yields, collection efficiencies (CE1 and CE2), cell losses were calculated. Engraftment data of 17 autologous transplantations were collected.
  
  
  RESULTS
  Apheresis duration was 239 min for a product volume of 220 mL. Cell product haematocrit, MNC and platelets counts were acceptable (respectively 2.4%, 65%, 834 x 109/L). Median platelet loss was 27.3%. Median CD34+ CE1 and CE2 were 64.6% and 48.5% respectively. We harvested 2.92 × 106CD34+ cells/kg, with a CD34 dose ≥ 2 × 106/kg for 67% of the procedures. Linear correlation between preapheresis CD34 count and the CP CD34 dose/kg allowed a prediction model with a decrease trend for high WBC precount. Procedures were well tolerated. For 17 autologous transplantations, median time to neutrophils and platelets reconstitutions were 12 and 13 days respectively.
  
  
  CONCLUSIONS
  Spectra Optia CMNC protocol successfully collected CD34+ cells with yields permitting the harvest of sufficient enriched grafts for autologous transplantation. The CD34+ cell yield prediction was excellent. PBSC collection with CMNC protocol had advantages of high processing rate, low product volume, and acceptable contamination by platelets. J. Clin. Apheresis 32:182-190, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: CHU La Conception, Service d'hémaphérèse Auto Transfusion.
authors.forename: F
authors.initials: F
authors.lastname: Sanderson
authors.affiliation: CHU La Conception, Service d'hémaphérèse Auto Transfusion.
authors.forename: P
authors.initials: P
authors.lastname: Poullin
authors.affiliation: Terumo BCT, Lakewood, Colorado, USA.
authors.forename: R
authors.initials: R
authors.lastname: Smith
authors.affiliation: CHU La Conception, Laboratoire d'hématologie.
authors.forename: C
authors.initials: C
authors.lastname: Nicolino-Brunet
authors.affiliation: Etablissement Français Du Sang Alpes Méditerranée, Saint Laurent du Var.
authors.forename: P
authors.initials: P
authors.lastname: Philip
authors.affiliation: Service d'hématologie oncologie, Aix-en-Provence.
authors.forename: A
authors.initials: A
authors.lastname: Chaib
authors.affiliation: CHU La Conception, Service d'hématologie et de thérapie cellulaire.
authors.forename: R
authors.initials: R
authors.lastname: Costello
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis; D001781:Blood Component Removal / Q000295:instrumentation* / Q000379:methods; D006085:Graft Survival; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D036102:Peripheral Blood Stem Cell Transplantation; D000072916:Peripheral Blood Stem Cells / Q000166:cytology; D012189:Retrospective Studies; D014182:Transplantation, Autologous
chemical_list: D018952:Antigens, CD34
keywords: apheresis; autologous peripheral blood stem-cell transplantation; neutrophil recovery; optia CMNC; peripheral blood stem-cell collection