title: Effects of protein and phosphate buffer concentrations on thermal denaturation of lysozyme analyzed by isoconversional method.
abstract: Thermal denaturation of lysozymes was studied as a function of protein concentration, phosphate buffer concentration, and scan rate using differential scanning calorimetry (DSC), which was then analyzed by the isoconversional method. The results showed that lysozyme thermal denaturation was only slightly affected by the protein concentration and scan rate. When the protein concentration and scan rate increased, the denaturation temperature (Tm) also increased accordingly. On the contrary, the Tm decreased with the increase of phosphate buffer concentration. The denaturation process of lysozymes was accelatated and the thermal stability was reduced with the increase of phosphate concentration. One part of degeneration process was not reversible where the aggregation occurred. The other part was reversible. The apparent activation energy (Ea) was computed by the isoconversional method. It decreased with the increase of the conversion ratio (α). The observed denaturation process could not be described by a simple reaction mechanism. It was not a process involving 2 standard reversible states, but a multi-step process. The new opportunities for investigating the kinetics process of protein denaturation can be supplied by this novel isoconversional method.
authors.affiliation: a Changsha Environmental Protection College , Changsha , China.
authors.forename: X M
authors.initials: XM
authors.lastname: Cao
authors.affiliation: b Environmental Monitoring Center of Hunan Province , Changsha , China.|c State Environmental Protection Key Laboratory of Monitoring for Heavy Metal Pollutants , Changsha , China.
authors.forename: Y
authors.initials: Y
authors.lastname: Tian
authors.affiliation: d College of Chemistry and Molecular Science; Wuhan University , Wuhan , China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: d College of Chemistry and Molecular Science; Wuhan University , Wuhan , China.
authors.forename: Y W
authors.initials: YW
authors.lastname: Liu
authors.affiliation: d College of Chemistry and Molecular Science; Wuhan University , Wuhan , China.
authors.forename: C X
authors.initials: CX
authors.lastname: Wang
mesh_terms: D002021:Buffers; D002152:Calorimetry, Differential Scanning; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008962:Models, Theoretical; D009113:Muramidase / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry*
chemical_list: D002021:Buffers; D010710:Phosphates; D011506:Proteins; D009113:Muramidase
keywords: DSC; isoconversional method; lysozyme; multi-step process; thermal denaturation