title: Effect of Genetic Diversity in Swine Leukocyte Antigen-DRA Gene on Piglet Diarrhea.
abstract: The swine leukocyte antigens (SLAs) are the multigene families related to immune responses. Little is known about the effect of the DRA gene on diarrheal disease. This study reported the genetic diversity of the DRA gene in exons 1, 3 and 4 in 290 Chinese Yantai black pigs. No variation was identified in exon 3. In exon 1, three genotypes and two alleles were identified, generated by two single nucleotide polymorphisms (SNPs). In exon 4, there were eight genotypes and five alleles containing seven SNPs were detected with four SNPs being novel SNPs. The low polymorphism found in swine DRA is consistent with the concept that the DRA gene is highly conserved among all mammalian species. Statistical analyses indicated that the genotypes of exon 1 were not significantly associated with piglet diarrhea (p > 0.05); however, genotypes C₄C₄ (1.80 ± 0.33) and A₄E₄ (1.66 ± 0.25) of exon 4 were significantly susceptible to diarrhea (p < 0.01). These indicate that the particular genotypes of the DRA gene are susceptible to diarrheal disease, which provides valuable information for disease-resistance breeding in swine.
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China. huanghxy100@163.com.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China. yangql0112@163.com.
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Ruzhou Animal Husbandry Bureau, Ruzhou 467500, China. 18893703880@163.com.
authors.forename: Junhu
authors.initials: J
authors.lastname: Yuan
authors.affiliation: College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730070, China. skllx@xbmu.edu.cn.
authors.forename: Lixia
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China. sun_china@outlook.com.
authors.forename: Wenyang
authors.initials: W
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China. 15600660313@163.com.
authors.forename: Yingdi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China. zhaosg@gsau.edu.cn.
authors.forename: Shengguo
authors.initials: S
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China. zhangsw66@sina.cn.
authors.forename: Shengwei
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China. huangwangz@126.com.
authors.forename: Wangzhou
authors.initials: W
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China. gunsbao056@126.com.|Gansu Research Center for Swine Production Engineering and Technology, Lanzhou 730070, China. gunsbao056@126.com.
authors.forename: Shuangbao
authors.initials: S
authors.lastname: Gun
keywords: PCR-SSCP; SLA-DRA; diarrhea; genetic diversity; swine