title: Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-α Production in PBMC of Patients with Behçet's Disease.
abstract: Behçet's disease (BD) is a multisystemic chronic inflammatory disease characterized by relapsing oral and genital ulcers, uveitis, and skin lesions. The pathogenesis of BD is still unknown. Aberrant production of some cytokines/chemokines plays an important role in the pathogenesis of various inflammatory diseases. Revealing a key signaling regulatory mechanism involved in proinflammatory cytokines/chemokines production is critical for understanding of the pathogenesis of BD. The aim of this study was to determine the role of Src family kinases (SFKs) in production of some LPS-induced proinflammatory cytokines/chemokines in peripheral blood mononuclear cells (PBMC) of active BD patients. Chemical inhibition of SFKs activity impaired LPS-induced TNF-α production in PBMC of active BD patients, suggesting that modulating SFKs activity may be a potential target for BD treatment.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Dicle University, 21280 Diyarbakır, Turkey.
authors.forename: Sevgi
authors.identifier: 0000-0001-6160-5626
authors.initials: S
authors.lastname: Irtegun
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Dicle University, 21280 Diyarbakır, Turkey.
authors.forename: Gulsum
authors.initials: G
authors.lastname: Pektanc
authors.affiliation: Department of Dermatology, Faculty of Medicine, Dicle University, 21280 Diyarbakır, Turkey.
authors.forename: Zeynep M
authors.initials: ZM
authors.lastname: Akkurt
authors.affiliation: Department of Physical Therapy and Rehabilitation, Faculty of Medicine, Dicle University, 21280 Diyarbakır, Turkey.
authors.forename: Mehtap
authors.initials: M
authors.lastname: Bozkurt
authors.affiliation: Department of Ophthalmology, Faculty of Medicine, Dicle University, 21280 Diyarbakır, Turkey.
authors.forename: Fatih M
authors.initials: FM
authors.lastname: Turkcu
authors.affiliation: Department of Immunology, Faculty of Medicine, Dicle University, 21280 Diyarbakır, Turkey.
authors.forename: Sevgi
authors.initials: S
authors.lastname: Kalkanli-Tas
mesh_terms: D000328:Adult; D001528:Behcet Syndrome / Q000378:metabolism*; D015153:Blotting, Western; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D055815:Young Adult; D019061:src-Family Kinases / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D019061:src-Family Kinases