title: The Effects of Aquaporin-1 in Pulmonary Edema Induced by Fat Embolism Syndrome.
abstract: This study was designed to investigate the role of aquaporin1 (AQP1) in the pathologic process of pulmonary edema induced by fat embolism syndrome (FES) and the effects of a free fatty acid (FFA) mixture on AQP1 expression in pulmonary microvascular endothelial cells (PMVECs). In vivo, edema was more serious in FES mice compared with the control group. The expression of AQP1 and the wet-to-dry lung weight ratio (W/D) in the FES group were significantly increased compared with the control group. At the same time, inhibition of AQP1 decreased the pathological damage resulting from pulmonary edema. Then we performed a study in vitro to investigate whether AQP1 was induced by FFA release in FES. The mRNA and protein level of AQP1 were increased by FFAs in a dose- and time-dependent manner in PMVECs. In addition, the up-regulation of AQP1 was blocked by the inhibitor of p38 kinase, implicating the p38 MAPK pathway as involved in the FFA-induced AQP1 up-regulation in PMVECs. Our results demonstrate that AQP1 may play important roles in pulmonary edema induced by FES and can be regarded as a new therapy target for treatment of pulmonary edema induced by FES.
authors.affiliation: Department of Anesthesiology, the Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China. 1030218497@sjtu.edu.cn.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, the Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China. tiankun5282@126.com.
authors.forename: Kun
authors.initials: K
authors.lastname: Tian
authors.affiliation: Department of Anesthesiology, the Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China. wangyanxq1989@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, the Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China. lotuszhangrong@163.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, the Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China. shangjiawei@sina.com.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Shang
authors.affiliation: Department of Anesthesiology, the Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China. jiangw@sjtu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Anesthesiology, the Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China. w19680420@sohu.com.
authors.forename: Aizhong
authors.initials: A
authors.lastname: Wang
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002478:Cells, Cultured; D004620:Embolism, Fat / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008168:Lung / Q000098:blood supply / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D055806:Microvessels / Q000166:cytology / Q000378:metabolism; D009929:Organ Size; D011654:Pulmonary Edema / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D034741:RNA, Small Interfering; D051398:Aquaporin 1
keywords: AQP1; fat embolism syndrome; free fatty acid; p38 MAPK signaling pathway; pulmonary edema