title: Ultrahigh resolution metabolomics for S-containing metabolites.
abstract: The advent of the genome-editing era greatly increases the opportunities for synthetic biology research that aims to enhance production of potentially useful bioactive metabolites in heterologous hosts. A wide variety of sulfur (S)-containing metabolites (S-metabolites) are known to possess bioactivities and health-promoting properties, but finding them and their chemical assignment using mass spectrometry-based metabolomics has been difficult. In this review, we highlight recent advances on the targeted metabolomic analysis of S-metabolites (S-omics) in plants using ultrahigh resolution mass spectrometry. The use of exact mass and signal intensity differences between32S-containing monoisotopic ions and counterpart34S isotopic ions exploits an entirely new method to characterize S-metabolites. Finally, we discuss the availability of S-omics for synthetic biology.
authors.affiliation: RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. Electronic address: ryo.nakabayashi@riken.jp.
authors.forename: Ryo
authors.initials: R
authors.lastname: Nakabayashi
authors.affiliation: RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan; Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan. Electronic address: kazuki.saito@riken.jp.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
mesh_terms: D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D010944:Plants / Q000378:metabolism*; D013455:Sulfur / Q000032:analysis*; D013460:Sulfur Isotopes / Q000032:analysis*
chemical_list: D013460:Sulfur Isotopes; D013455:Sulfur