title: Aluminum Toxicity-Induced Alterations of Leaf Proteome in Two Citrus Species Differing in Aluminum Tolerance.
abstract: Seedlings of aluminum-tolerant 'Xuegan' (Citrus sinensis) and Al-intolerant 'sour pummelo' (Citrus grandis) were fertigated for 18 weeks with nutrient solution containing 0 and 1.2 mM AlCl₃·6H₂O. Al toxicity-induced inhibition of photosynthesis and the decrease of total soluble protein only occurred in C. grandis leaves, demonstrating that C. sinensis had higher Al tolerance than C. grandis. Using isobaric tags for relative and absolute quantification (iTRAQ), we obtained more Al toxicity-responsive proteins from C. sinensis than from C. grandis leaves, which might be responsible for the higher Al tolerance of C. sinensis. The following aspects might contribute to the Al tolerance of C. sinensis: (a) better maintenance of photosynthesis and energy balance via inducing photosynthesis and energy-related proteins; (b) less increased requirement for the detoxification of reactive oxygen species and other toxic compounds, such as aldehydes, and great improvement of the total ability of detoxification; and (c) upregulation of low-phosphorus-responsive proteins. Al toxicity-responsive proteins related to RNA regulation, protein metabolism, cellular transport and signal transduction might also play key roles in the higher Al tolerance of C. sinensis. We present the global picture of Al toxicity-induced alterations of protein profiles in citrus leaves, and identify some new Al toxicity-responsive proteins related to various biological processes. Our results provide some novel clues about plant Al tolerance.
authors.affiliation: Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. lihuanenglish123@126.com.|Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. lihuanenglish123@126.com.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. talstoy@163.com.|Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. talstoy@163.com.
authors.forename: Lin-Tong
authors.initials: LT
authors.lastname: Yang
authors.affiliation: Institute of Materia Medica, Fujian Academy of Medical Sciences, Fuzhou 350001, China. qiyiping2008@hotmail.com.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Qi
authors.affiliation: Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. 6253730@163.com.|Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. 6253730@163.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.affiliation: Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. yibin.07@163.com.|Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. yibin.07@163.com.
authors.forename: Yi-Bin
authors.initials: YB
authors.lastname: Lu
authors.affiliation: Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. lisongchen2002@hotmail.com.|Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. lisongchen2002@hotmail.com.|The Higher Educational Key Laboratory of Fujian Province for Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry University, Fuzhou 350002, China. lisongchen2002@hotmail.com.
authors.forename: Li-Song
authors.initials: LS
authors.lastname: Chen
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D032084:Citrus sinensis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D019295:Computational Biology; D004361:Drug Tolerance; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000187:drug effects*; D013045:Species Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins; D020543:Proteome; D000535:Aluminum
keywords: Citrus grandis; Citrus sinensis; aluminum toxicity; iTRAQ; leaves; proteome