title: Emergence of Mycoplasma genitalium strains showing mutations associated with macrolide and fluoroquinolone resistance in the region Dresden, Germany.
abstract: Among 323 specimens from male patients with symptoms of non-gonococcal urethritis, Mycoplasma genitalium was detected in 19 samples by real-time PCR. Mutations of 23S rRNA gene associated with macrolide resistance were confirmed in 10 strains. Amino acid changes at positions 81 and 83 of ParC protein were demonstrated indicating quinolone resistance of two strains.
authors.affiliation: TU Dresden, Institute of Medical Microbiology and Hygiene, Dresden, Germany. Electronic address: roger.dumke@tu-dresden.de.
authors.forename: Roger
authors.initials: R
authors.lastname: Dumke
authors.affiliation: TU Dresden, Institute of Medical Microbiology and Hygiene, Dresden, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Thürmer
authors.affiliation: TU Dresden, Institute of Medical Microbiology and Hygiene, Dresden, Germany.
authors.forename: Enno
authors.initials: E
authors.lastname: Jacobs
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D024841:Fluoroquinolones / Q000494:pharmacology*; D005838:Genotype; D005858:Germany; D006801:Humans; D018942:Macrolides / Q000494:pharmacology*; D008297:Male; D009154:Mutation; D009175:Mycoplasma Infections / Q000382:microbiology; D045704:Mycoplasma genitalium / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D024841:Fluoroquinolones; D018942:Macrolides; D012338:RNA, Ribosomal, 23S
keywords: 23S rRNA mutation; Genotyping; Macrolide resistance; Mycoplasma genitalium; Quinolone resistance; Topoisomerase mutation