title: Application of residual polysaccharide-degrading enzymes in dried shiitake mushrooms as an enzyme preparation in food processing.
abstract: OBJECTIVE
  To examine the activities of residual enzymes in dried shiitake mushrooms, which are a traditional foodstuff in Japanese cuisine, for possible applications in food processing.
  
  
  RESULTS
  Polysaccharide-degrading enzymes remained intact in dried shiitake mushrooms and the activities of amylase, β-glucosidase and pectinase were high. A potato digestion was tested using dried shiitake powder. The enzymes reacted with potato tuber specimens to solubilize sugars even under a heterogeneous solid-state condition and that their reaction modes were different at 38 and 50 °C.
  
  
  CONCLUSIONS
  Dried shiitake mushrooms have a potential use in food processing as an enzyme preparation.
authors.affiliation: Faculty of Life and Environmental Science, Kyoto Prefectural University, Shimogamo-nakaragi-cho, Sakyo-ku, Kyoto, 606-8522, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Tatsumi
authors.affiliation: The Department of Food and Nutrition, Koran Women's Junior College, Yokote 1-2-1, Minami-ku, Fukuoka, 811-1311, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Konishi
authors.affiliation: Graduate School of Life and Environmental Science, Kyoto Prefectural University, Shimogamo-nakaragi-cho, Kyoto, 606-8522, Japan. s_tsuji@kpu.ac.jp.
authors.forename: S
authors.initials: S
authors.lastname: Tsujiyama
mesh_terms: D000681:Amylases / Q000378:metabolism; D050260:Carbohydrate Metabolism; D005511:Food Handling; D062610:Fungal Polysaccharides / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D011096:Polygalacturonase / Q000378:metabolism; D020942:Shiitake Mushrooms / Q000201:enzymology*; D011198:Solanum tuberosum / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D062610:Fungal Polysaccharides; D005656:Fungal Proteins; D000681:Amylases; D011096:Polygalacturonase; D001617:beta-Glucosidase
keywords: Amylase; Cellulase; Dried shiitake mushroom; Food processing; Lentinus edodes; Pectinase; Shiitake enzymes