title: Design and fabrication of 3D-printed anatomically shaped lumbar cage for intervertebral disc (IVD) degeneration treatment.
abstract: Spinal fusion is the gold standard surgical procedure for degenerative spinal conditions when conservative therapies have been unsuccessful in rehabilitation of patients. Novel strategies are required to improve biocompatibility and osseointegration of traditionally used materials for lumbar cages. Furthermore, new design and technologies are needed to bridge the gap due to the shortage of optimal implant sizes to fill the intervertebral disc defect. Within this context, additive manufacturing technology presents an excellent opportunity to fabricate ergonomic shape medical implants. The goal of this study is to design and manufacture a 3D-printed lumbar cage for lumbar interbody fusion. Optimisations of the proposed implant design and its printing parameters were achieved via in silico analysis. The final construct was characterised via scanning electron microscopy, contact angle, x-ray micro computed tomography (μCT), atomic force microscopy, and compressive test. Preliminary in vitro cell culture tests such as morphological assessment and metabolic activities were performed to access biocompatibility of 3D-printed constructs. Results of in silico analysis provided a useful platform to test preliminary cage design and to find an optimal value of filling density for 3D printing process. Surface characterisation confirmed a uniform coating of nHAp with nanoscale topography. Mechanical evaluation showed mechanical properties of final cage design similar to that of trabecular bone. Preliminary cell culture results showed promising results in terms of cell growth and activity confirming biocompatibility of constructs. Thus for the first time, design optimisation based on computational and experimental analysis combined with the 3D-printing technique for intervertebral fusion cage has been reported in a single study. 3D-printing is a promising technique for medical applications and this study paves the way for future development of customised implants in spinal surgical applications.
authors.affiliation: Division of Surgery & Interventional Science, Royal Free Hospital Campus, UCL, NW3 2PF, UK.
authors.forename: T
authors.initials: T
authors.lastname: Serra
authors.forename: C
authors.initials: C
authors.lastname: Capelli
authors.forename: R
authors.initials: R
authors.lastname: Toumpaniari
authors.forename: I R
authors.initials: IR
authors.lastname: Orriss
authors.forename: J J H
authors.initials: JJ
authors.lastname: Leong
authors.forename: K
authors.initials: K
authors.lastname: Dalgarno
authors.forename: D M
authors.initials: DM
authors.lastname: Kalaskar
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D001672:Biocompatible Materials; D019245:Compressive Strength; D003198:Computer Simulation; D006801:Humans; D018625:Microscopy, Atomic Force; D008954:Models, Biological; D066330:Printing, Three-Dimensional; D019736:Prostheses and Implants; D013123:Spinal Fusion / Q000295:instrumentation*; D013234:Stem Cells / Q000502:physiology; D013314:Stress, Mechanical
chemical_list: D001672:Biocompatible Materials