title: Amperometric Glucose Biosensor Based on Effective Self-Assembly Technology for Preparation of Poly(allylamine hydrochloride)/Au Nanoparticles Multilayers.
abstract: Novel nanomaterials and nanotechnology for use in bioassay applications represent a rapidly advancing field. This study developed a novel method to fabricate the glucose biosensor with good gold nanoparticles (AuNPs) fixed efficiency based on effective self-assembly technology for preparation of multilayers composed of poly(allylamine hydrochloride) (PAH) and AuNPs. The electrochemical properties of the biosensor based on (AuNPs/PAH)n/AuNPs/glucose oxide (GOD) with different multilayers were systematically investigated. Among the resulting glucose biosensors, electrochemical properties of the biosensor with three times self-assembly processes ((AuNPs/PAH)3/AuNPs/GOD) is best. The GOD biosensor exhibited a fast amperometric response (5 s) to glucose, a good linear current-time relation over a wide range of glucose concentrations from 0.05 to 162 mM, and a low detection limit of 0.029 mM. The GOD biosensor modified with (AuNPs/PAH)n layers will have essential significance and practical application in future owing to the simple method of fabrication and good performance.
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Ye
authors.forename: Hangqing
authors.initials: H
authors.lastname: Xie
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Shao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wei
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Liu
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhao
authors.forename: Xinyi
authors.initials: X
authors.lastname: Xia
mesh_terms: D000499:Allylamine / Q000737:chemistry*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D005947:Glucose / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011108:Polymers; D000499:Allylamine; D006046:Gold; D005947:Glucose