title: Atomic resolution structure determination by the cryo-EM method MicroED.
abstract: The electron cryo-microscopy (cryoEM) method MicroED has been rapidly developing. In this review we highlight some of the key steps in MicroED from crystal analysis to structure determination. We compare and contrast MicroED and the latest X-ray based diffraction method the X-ray free-electron laser (XFEL). Strengths and shortcomings of both MicroED and XFEL are discussed. Finally, all current MicroED structures are tabulated with a view to the future.
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia, 20148.
authors.forename: Shian
authors.initials: S
authors.lastname: Liu
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia, 20148.
authors.forename: Johan
authors.initials: J
authors.lastname: Hattne
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia, 20148.
authors.forename: Francis E
authors.initials: FE
authors.lastname: Reyes
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia, 20148.
authors.forename: Silvia
authors.initials: S
authors.lastname: Sanchez-Martinez
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia, 20148.
authors.forename: M
authors.initials: M
authors.lastname: Jason de la Cruz
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia, 20148.
authors.forename: Dan
authors.initials: D
authors.lastname: Shi
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia, 20148.
authors.forename: Tamir
authors.initials: T
authors.lastname: Gonen
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods*; D003461:Crystallography / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D053758:Nanoparticles / Q000648:ultrastructure*
keywords: MicroED; cryo EM; crystallography; nano crystals