title: Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics.
abstract: The skin provides protection against environmental stress. However, intrinsic and extrinsic aging causes significant alteration to skin structure and components, which subsequently impairs molecular characteristics and biochemical processes. Here, we have conducted an immunohistological investigation and established the proteome profiles on nude mice skin to verify the specific responses during aging caused by different factors. Our results showed that UVB-elicited aging results in upregulation of proliferating cell nuclear antigen and strong oxidative damage in DNA, whereas chronological aging abolished epidermal cell growth and increased the expression of caspase-14, as well as protein carbonylation. Network analysis indicated that the programmed skin aging activated the ubiquitin system and triggered obvious downregulation of 14-3-3 sigma, which might accelerate the loss of cell growth capacity. On the other hand, UVB stimulation enhanced inflammation and the risk of skin carcinogenesis. Collectively, functional proteomics could provide large-scale investigation of the potent proteins and molecules that play important roles in skin subjected to both intrinsic and extrinsic aging.
authors.affiliation: Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Taoyuan, Taiwan.|Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Jia-You
authors.initials: JY
authors.lastname: Fang
authors.affiliation: School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Pei-Wen
authors.initials: PW
authors.lastname: Wang
authors.affiliation: Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
authors.forename: Chun-Hsun
authors.initials: CH
authors.lastname: Huang
authors.affiliation: Department of Psychiatry, Taipei Veterans General Hospital, Taipei, Taiwan.|Department of Psychiatry, College of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Mu-Hong
authors.initials: MH
authors.lastname: Chen
authors.affiliation: Graduate Institute of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Yun-Ru
authors.initials: YR
authors.lastname: Wu
authors.affiliation: School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan. pan@mail.cgu.edu.tw.|Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan. pan@mail.cgu.edu.tw.|Liver Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan. pan@mail.cgu.edu.tw.|Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan, Taiwan. pan@mail.cgu.edu.tw.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan. pan@mail.cgu.edu.tw.
authors.forename: Tai-Long
authors.initials: TL
authors.lastname: Pan
mesh_terms: D048948:14-3-3 Proteins / Q000032:analysis / Q000378:metabolism; D000375:Aging; D000818:Animals; D002454:Cell Differentiation / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D005260:Female; D008819:Mice, Nude; D018384:Oxidative Stress / Q000528:radiation effects; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics; D012867:Skin / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D015595:Skin Aging; D014452:Ubiquitins / Q000032:analysis / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D048948:14-3-3 Proteins; D020543:Proteome; C499130:Sfn protein, mouse; D014452:Ubiquitins
keywords: Animal Proteomics; Chronological; Network analysis; Proteome; UVB; Ubiquitin