title: Magnetic Nano-Sponges for High-Capacity Protein Enrichment and Immobilization.
abstract: On the basis of the gel effect of the living RAFT (Reversible Addition-Fragmentation Chain Transfer) polymerization, magnetic nano-sponges with ultrasoft PAA net-like shells are prepared and then used for enriching the entire proteins from real biological samples with ultrahigh capacity. In addition, the immobilized enzyme Cyt c or HRP surprisingly show much higher catalytic activity than free enzymes.
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science and Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, P. R. China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science and Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, P. R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science and Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, P. R. China.
authors.forename: Zihao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science and Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, P. R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science and Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, P. R. China. ccwang@fudan.edu.cn.
authors.forename: Changchun
authors.initials: C
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism*; D060328:Magnetic Phenomena; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011817:Rabbits; D014466:Ultraviolet Rays
chemical_list: D055767:Immobilized Proteins
keywords: 3D functional structures; enhanced catalytic performance; high enrichment capacity; nano-sponges; ultrasoft network