title: Propolis Inhibits Neurite Outgrowth in Differentiating SH-SY5Y Human Neuroblastoma Cells.
abstract: Propolis is a multicomponent, active, complex resinous substance collected by honeybees from a variety of plant sources. We have studied the effect of propolis on neurite outgrowth of SH-SY5Y human neuroblastoma cells induced to differentiate by all-trans-retinoic acid (RA). Propolis, at a concentration of 3 μg/mL, had no significant effect on the viability of differentiating SH-SY5Y cells. However, the neurite outgrowth of the differentiating SH-SY5Y cells treated with propolis (0.3~3 μg/mL) for 48 hr was significantly inhibited in a dose-dependent manner. Treatment of RA-stimulated differentiating SH-SY5Y cells with 0.3 to 3 μg/mL propolis resulted in decreased level of transglutaminase and 43-kDa growth-associated protein (GAP-43) in a dose-dependent manner. The results indicate that propolis is able to inhibit neurite outgrowth of differentiating SH-SY5Y cells.
authors.affiliation: Dept. of Life Science, Kyonggi University, Suwon, Korea.
authors.forename: Han Bit
authors.initials: HB
authors.lastname: Kim
authors.affiliation: Dept. of Life Science, Kyonggi University, Suwon, Korea.
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Yoo
keywords: GAP-43; Neurite outgrowth; Propolis; SH-SY5Y neuroblastoma cell; TGase