title: Protocols for Gelfoam(®) Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Containing Nestin-GFP-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells for Long Time Periods.
abstract: Gelfoam(®)-histocultured whisker follicles from nestin-driven-green fluorescent protein (ND-GFP) mice produced growing pigmented and unpigmented hair shafts. Hair-shaft length increased rapidly by day 4 and continued growing until at least day 12 after which the hair-shaft length was constant. By day 63 in histoculture, the number of ND-GFP hair follicle-associated pluripotent (HAP) stem cells increased significantly and the follicles were intact. Three-dimensional Gelfoam(®) histoculture of hair follicles can provide a very long-term period for evaluating novel agents to promote hair growth.
authors.affiliation: AntiCancer Inc., San Diego, CA, USA.|Department of Surgery, University of California, San Diego, San Diego, CA, USA.|Department of Anatomy, Institute of Biomedical Engineering, Second Military Medical University, Shanghai, China.
authors.forename: Wenluo
authors.initials: W
authors.lastname: Cao
authors.affiliation: AntiCancer Inc., San Diego, CA, USA.|Department of Anatomy, Institute of Biomedical Engineering, Second Military Medical University, Shanghai, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: AntiCancer Inc., San Diego, CA, USA.|Department of Surgery, University of California, San Diego, San Diego, CA, USA.|Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Amoh
authors.affiliation: AntiCancer Inc., San Diego, CA, USA. all@anticancer.com.|Department of Surgery, University of California, San Diego, San Diego, CA, USA. all@anticancer.com.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D005781:Gelatin Sponge, Absorbable; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology*; D051379:Mice; D064231:Nestin / Q000235:genetics*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D014738:Vibrissae
chemical_list: D064231:Nestin; D049452:Green Fluorescent Proteins
keywords: GFP; Gelfoam®; HAP stem cells; Hair-shaft growth; Nestin; Transgenic mice; Whisker culture