title: Hyperbaric oxygen preconditioning protects the lung against acute pancreatitis induced injury via attenuating inflammation and oxidative stress in a nitric oxide dependent manner.
abstract: This study aimed to investigate the protective effects of hyperbaric oxygen preconditioning (HBO-PC) on acute pancreatitis AP associated acute lung injury (ALI) and the potential mechanisms. Rats were randomly divided into sham group, AP group, HBO-PC + AP group and HBO-PC + L-NAME group. Rats in HBO-PC + AP group received HBO-PC once daily for 3 days, and AP was introduced 24 h after last HBO-PC. In HBO-PC + L-NAME group, L-NAME (40 mg/kg) was intraperitoneally injected before each HBO-PC. At 24 h after AP, the blood lipase and amylase activities were measured; the lung and pancreas were harvested for pathological examination; the bronchoalveolar lavage fluid was collected for the detection of lactate dehydrogenase (LDH) and proteins; inflammatory factors, superoxide dismutase (SOD) activity and malonaldehyde content were measured in the lung and blood; the Nrf2, SOD-1 and haem oxygenase-1 (HO-1) protein expression was measured in the lung. The lung nitric oxide (NO) and NO synthase activity increased significantly after HBO-PC. HBO-PC was able to reduce blood lipase and amylase activities, improve lung and pancreatic pathology, decrease LDH and proteins in BALF, inhibit the production of inflammatory factors, reduce malonaldehyde content and increase SOD activity in the lung and blood as well as increase protein expression of Nrf2, SOD-1 and HO-1 in the lung. However, L-NAME before HBO-PC significantly attenuated protective effects of HBO-PC. HBO-PC is able to protect the lung against AP induced injury by attenuating inflammation and oxidative stress in the lung via a NO dependent manner.
authors.affiliation: Department of Gastroenterology, Changhai Hospital, The Second Military Medical University, Shanghai, PR China.
authors.forename: Qi-Hong
authors.initials: QH
authors.lastname: Yu
authors.affiliation: Department of Cardiothoracic Surgery, The First Hospital of Jining City, No 6, Jiankang Road, Jining City, Shandong, 272011, PR China.
authors.forename: Pei-Xi
authors.initials: PX
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Yantaishan Hospital, No 91, Jiefang Road, Zhigang District, Yantai City, Shandong, 264001, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Diving and Hyperbaric Medicine, The Second Military Medical University, Shanghai, PR China. Electronic address: liuwenwu1980@hotmail.com.
authors.forename: Wenwu
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology & Critical Care Medicine, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, PR China. Electronic address: anne_yin2016@163.com.
authors.forename: Na
authors.initials: N
authors.lastname: Yin
mesh_terms: D000818:Animals; D006931:Hyperbaric Oxygenation / Q000379:methods*; D007249:Inflammation / Q000209:etiology* / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D008168:Lung / Q000276:immunology / Q000473:pathology; D055370:Lung Injury / Q000209:etiology* / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D008297:Male; D009569:Nitric Oxide / Q000032:analysis / Q000276:immunology; D018384:Oxidative Stress; D010179:Pancreas / Q000276:immunology / Q000473:pathology; D010195:Pancreatitis / Q000150:complications* / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D017207:Rats, Sprague-Dawley
chemical_list: D009569:Nitric Oxide
keywords: Acute pancreatitis associated lung injury; Hyperbaric oxygen preconditioning; Inflammation; Nitric oxide; Oxidative stress