title: Lowering photosensitizer doses and increasing fluences induce apoptosis in tumor bearing mice.
abstract: The objective of this study was to determine an optimal dose of photodynamic therapy (PDT) for inducing apoptotic tumor cells in vivo. In this context, mice bearing human tongue-squamous epithelium carcinomas were treated with various photosensitizer concentrations and fluences. Tumor apoptosis was imaged after 2 days via a self-designed DY-734-annexin V probe using near-infrared fluorescence (NIRF) optical imaging. Apoptosis was verified ex vivo via TUNEL staining. Apoptotic tumor cells were detected in vivo at a dose of 40 µg photosensitizer and a fluency of 100 J/cm(2). This is the lowest photosensitizer dose reported so far.
authors.affiliation: Experimental Radiology, Institute of Diagnostic and Interventional Radiology, Jena University Hospital, Friedrich-Schiller University Jena, Erlanger Allee 101, D-07747 Jena, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Haedicke
authors.affiliation: Biolitec Research GmbH, Research & Development, Otto-Schott-Straße 15, D-07745 Jena, Germany.
authors.forename: Susanna
authors.initials: S
authors.lastname: Graefe
authors.affiliation: Experimental Radiology, Institute of Diagnostic and Interventional Radiology, Jena University Hospital, Friedrich-Schiller University Jena, Erlanger Allee 101, D-07747 Jena, Germany.
authors.forename: Ulf
authors.initials: U
authors.lastname: Teichgraeber
authors.affiliation: Experimental Radiology, Institute of Diagnostic and Interventional Radiology, Jena University Hospital, Friedrich-Schiller University Jena, Erlanger Allee 101, D-07747 Jena, Germany.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Hilger
keywords: (000.1430) Biology and medicine; (170.0110) Imaging systems; (170.0170) Medical optics and biotechnology; (170.5380) Physiology