title: Association of APOA5 Gene Promoter Region -1131T>C Polymorphism (rs662799) to Plasma Triglyceride Level in Patients with Type 2 Diabetic Nephropathy.
abstract: BACKGROUND
  Diabetic Nephropathy (DN), a serious complication of Type 2 Diabetic Mellitus (T2DM), is progressive and susceptibility to DN varies among T2DM patients. ApoA5-1131T>C polymorphism revealed that is strongly associated with triglyceride levels and proposed as a predisposing factor for DN.
  
  
  OBJECTIVE
  The purpose of this study was to investigate the association -1131T>C ApoA5 gene polymorphism with serum lipids levels in Type 2 diabetic (DM) patients with or without DN in north of Iran (Mazandaran province).
  
  
  METHODS
  This study comprised patients with established T2DM (n=161) and controls (n=58). Genotyping of APOA5 -1131T>C polymorphisms was performed by PCR-RFLP. Diabetic patients were divided into two groups: with nephropathy (DN+, n = 90) and without nephropathy (DN-, n = 71). Lipids and lipoproteins were assessed by enzymatic methods.
  
  
  RESULTS
  The genotype frequencies were 63.8 % TT, 31 % TC, 5.2 % CC in controls, 33.8% TT, 52.1 % TC, 14.1 % CC in DN- and 44.4 % TT, 36.7 % TC, 18.9 % CC in DN+ patients. The TC genotype and the CC genotype were overexpressed among DN+ and DN-population in comparison to the control group. The highest and the lowest TG levels in both diabetic patients and controls belonged to CC+TC and TT genotypes, respectively. Furthermore in both patients TG increased with this order: TT< TC<CC.
  
  
  CONCLUSIONS
  These results suggest that APOA5 -1131T>C polymorphisms influence lipid levels in type 2 diabetic patients.
authors.affiliation: Faculty of Medicine, Department of Biochemistry, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences , Sari, Iran .
authors.forename: Abdolkarim
authors.initials: A
authors.lastname: Mahrooz
authors.affiliation: Faculty of Medicine, Department of Biochemistry, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences , Sari, Iran .
authors.forename: Mehryar
authors.initials: M
authors.lastname: Zargari
authors.affiliation: Faculty of Medicine, Department of Biochemistry, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences , Sari, Iran .
authors.forename: Vahid
authors.initials: V
authors.lastname: Ansari
authors.affiliation: Faculty, Department of Nephrology, Imam Khomeini Hospital, Mazandaran University of Medical Sciences , Sari, IR Iran .
authors.forename: Atieh
authors.initials: A
authors.lastname: Makhlough
authors.affiliation: Faculty of Medicine, Department of Biochemistry, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences , Sari, Iran .
authors.forename: Mohammad-Bagher
authors.initials: MB
authors.lastname: Hashemi-Sooteh
keywords: Genotyping; Kidney; Lipids; PCR-RFLP