title: Dendritic cells induce Tc1 cell differentiation via the CD40/CD40L pathway in mice after exposure to cigarette smoke.
abstract: Dendritic cells and CD8(+) T cells participate in the pathology of chronic obstructive pulmonary disease, including emphysema, but little is known of the involvement of the CD40/CD40L pathway. We investigated the role of the CD40/CD40L pathway in Tc1 cell differentiation induced by dendritic cells in a mouse model of emphysema, and in vitro. C57BL/6J wild-type and CD40(-/-) mice were exposed to cigarette smoke (CS) or not (control), for 24 wk. In vitro experiments involved wild-type and CD40(-/-) dendritic cells treated with CS extract (CSE) or not. Compared with the control groups, the CS mice (both wild type and CD40(-/-)) had a greater percentage of lung dendritic cells and higher levels of major histocompatability complex (MHC) class I molecules and costimulatory molecules CD40 and CD80. Relative to the CS CD40(-/-) mice, the CS wild type showed greater signs of lung damage and Tc1 cell differentiation. In vitro, the CSE-treated wild-type cells evidenced more cytokine release (IL-12/p70) and Tc1 cell differentiation than did the CSE-treated CD40(-/-) cells. Exposure to cigarette smoke increases the percentage of lung dendritic cells and promotes Tc1 cell differentiation via the CD40/CD40L pathway. Blocking the CD40/CD40L pathway may suppress development of emphysema in mice exposed to cigarette smoke.
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; and.
authors.forename: Liang-Jian
authors.initials: LJ
authors.lastname: Kuang
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; and.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Deng
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; and.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; and.
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Qiu
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; and.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; and.
authors.forename: Zhi-Yi
authors.initials: ZY
authors.lastname: He
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; and.
authors.forename: Jian-Quan
authors.initials: JQ
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; and.
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; and.
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; and.
authors.forename: Jing-Min
authors.initials: JM
authors.lastname: Deng
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; and.
authors.forename: Guang-Nan
authors.initials: GN
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, Tenth Affiliated Hospital of Guangxi Medical University, Qinzhou, Guangxi, China.
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; and xnzhong101@sina.com.
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Zhong
mesh_terms: D000818:Animals; D019013:CD40 Antigens / Q000502:physiology*; D023201:CD40 Ligand / Q000502:physiology*; D018414:CD8-Positive T-Lymphocytes; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D003713:Dendritic Cells / Q000502:physiology*; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018655:Lymphocyte Count; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011656:Pulmonary Emphysema / Q000209:etiology / Q000276:immunology* / Q000378:metabolism; D015398:Signal Transduction; D012906:Smoke / Q000009:adverse effects*; D012907:Smoking / Q000009:adverse effects; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000009:adverse effects; D000098815:T-bet Transcription Factor
chemical_list: D019013:CD40 Antigens; D016207:Cytokines; D012906:Smoke; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D023201:CD40 Ligand
keywords: CD40; Tc1 cell; cigarette smoke; dendritic cell; emphysema