title: Simultaneous determination of penflufen and one metabolite in vegetables and cereals using a modified quick, easy, cheap, effective, rugged, and safe method and liquid chromatography coupled to tandem mass spectrometry.
abstract: The present study attempts to investigate a rapid, effective and sensitive analytical method to determine a new fungicide penflufen and its metabolite residues in potato, cowpea, corn, wheat and rice using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). The compounds were extracted using acetonitrile and cleaned up by primary secondary amine (PSA) and octadecylsilane (C18). Both compounds were determined in less than 5.0min using an electrospray ionization source in positive mode (ESI+). The calculated limits of detection and quantification were below 0.018μgkg(-1) and 0.060μgkg(-1) in different matrices. The method revealed excellent linearity (R(2)>0.9957) for both compounds. The recoveries ranged from 76.1 to 101.0% for both compounds with RSD values lower than 9.8% in all matrices. The results demonstrated that the method is reliable and effective enough for the routine detecting penflufen and its metabolite residues in vegetables and cereals.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Henan Institute of Science and Technology, Xinxiang 453003, China.
authors.forename: Fajun
authors.initials: F
authors.lastname: Tian
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: liuxingang@caas.cn.
authors.forename: Xingang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Henan Institute of Science and Technology, Xinxiang 453003, China. Electronic address: wybhist@126.com.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Fengshou
authors.initials: F
authors.lastname: Dong
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D002523:Edible Grain / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D005659:Fungicides, Industrial / Q000032:analysis* / Q000378:metabolism*; D012275:Oryza / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors; D014675:Vegetables / Q000737:chemistry*
chemical_list: D005659:Fungicides, Industrial
keywords: Food; Metabolite; Penflufen; Residue; UHPLC–MS/MS