title: Dual-Modality Positron Emission Tomography/Optical Image-Guided Photodynamic Cancer Therapy with Chlorin e6-Containing Nanomicelles.
abstract: Multifunctional nanoparticles with combined diagnostic and therapeutic functions show great promise in nanomedicine. Herein, we develop an organic photodynamic therapy (PDT) system based on polyethylene glycol (PEG)-coated nanomicelles conjugated with ∼20% chlorin e6 (PEG-Ce 6 nanomicelles), which functions as an optical imaging agent, as well as a PDT agent. The formed PEG-Ce 6 nanomicelles with the size of ∼20 nm were highly stable in various physiological solutions for a long time. Moreover, Ce 6 can also be a (64)Cu chelating agent for in vivo positron emission tomography (PET). By simply mixing, more than 90% of (64)Cu was chelator-free labeled on PEG-Ce 6 nanomicelles, and they also showed high stability in serum conditions. Both fluorescence imaging and PET imaging revealed that PEG-Ce 6 nanomicelles displayed high tumor uptake (13.7 ± 2.2%ID/g) after intravenous injection into tumor-bearing mice at the 48 h time point. In addition, PEG-Ce 6 nanomicelles exhibited excellent PDT properties upon laser irradiation, confirming the theranostic properties of PEG-Ce 6 nanomicelles for imaging and treatment of cancer. In addition, PDT was not shown to render any appreciable toxicity. This work presents a theranostic platform based on polymer nanomicelles with great potential in multimodality imaging-guided photodynamic cancer therapy.
authors.affiliation: Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University , Suzhou, Jiangsu 215123, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Cheng
authors.forename: Anyanee
authors.initials: A
authors.lastname: Kamkaew
authors.forename: Haiyan
authors.initials: H
authors.lastname: Sun
authors.forename: Dawei
authors.initials: D
authors.lastname: Jiang
authors.forename: Hector F
authors.initials: HF
authors.lastname: Valdovinos
authors.affiliation: Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University , Suzhou, Jiangsu 215123, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Gong
authors.forename: Christopher G
authors.initials: CG
authors.lastname: England
authors.forename: Shreya
authors.initials: S
authors.lastname: Goel
authors.forename: Todd E
authors.initials: TE
authors.lastname: Barnhart
authors.forename: Weibo
authors.initials: W
authors.lastname: Cai
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002735:Chlorophyllides; D051379:Mice; D008823:Micelles; D053758:Nanoparticles; D010778:Photochemotherapy; D011166:Porphyrins; D049268:Positron-Emission Tomography
chemical_list: D002735:Chlorophyllides; D008823:Micelles; D011166:Porphyrins; C062985:phytochlorin
keywords: EPR effect; PEG-Ce 6 nanomicelles; PET imaging; fluorescence imaging; photodynamic therapy