title: Nestin-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term 3D-Gelfoam® Histoculture.
abstract: Mouse whiskers containing hair-follicle-associated pluripotent (HAP) stem cells, from nestin-driven green fluorescent protein (ND-GFP) transgenic mice, were placed in 3D histoculture supported by Gelfoam(®). β-III tubulin-positive fibers, consisting of ND-GFP-expressing HAP stem cells, extended up to 500 mm from the whisker nerve stump in histoculture. The growing fibers had growth cones on their tips expressing F-actin indicating they were growing axons. The growing whisker sensory nerve was highly enriched in ND-GFP HAP stem cells which appeared to play a major role in its elongation and interaction with other nerves placed in 3D culture, including the sciatic nerve, the trigeminal nerve, and the trigeminal nerve ganglion. The results suggested that a major function of HAP stem cells in the hair follicle is for growth of the hair follicle sensory nerve.
authors.affiliation: AntiCancer Inc., San Diego, CA, USA. miisumi@med.kitasato-u.ac.jp.|Department of Surgery, University of California, San Diego, San Diego, CA, USA. miisumi@med.kitasato-u.ac.jp.|Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan. miisumi@med.kitasato-u.ac.jp.
authors.forename: Sumiyuki
authors.initials: S
authors.lastname: Mii
authors.affiliation: AntiCancer Inc., San Diego, CA, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Duong
authors.affiliation: AntiCancer Inc., San Diego, CA, USA.|Department of Surgery, University of California, San Diego, San Diego, CA, USA.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Tome
authors.affiliation: AntiCancer Inc., San Diego, CA, USA.|Department of Biophotonics and Laser Technology, Saarland University, Saarbruecken, Germany.
authors.forename: Aisada
authors.initials: A
authors.lastname: Uchugonova
authors.affiliation: AntiCancer Inc., San Diego, CA, USA.|Department of Anatomy, Second Military Medical University, Shanghai, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Amoh
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.
authors.forename: Norimitsu
authors.initials: N
authors.lastname: Saito
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.
authors.forename: Kensei
authors.initials: K
authors.lastname: Katsuoka
authors.affiliation: AntiCancer Inc., San Diego, CA, USA.|Department of Surgery, University of California, San Diego, San Diego, CA, USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D005724:Ganglia / Q000166:cytology; D015870:Gene Expression; D018859:Hair Follicle / Q000166:cytology*; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D064231:Nestin / Q000235:genetics* / Q000378:metabolism; D059348:Peripheral Nerve Injuries; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology; D014738:Vibrissae / Q000378:metabolism*
chemical_list: D064231:Nestin
keywords: Confocal fluorescence imaging; GFP; Gelfoam®; Hair follicle; Histoculture; Nerve ganglion; Nestin; Peripheral nerve; Pluripotent; Stem cells; Vibrissa