title: In vitro culture of Echinococcus multilocularis producing protoscoleces and mouse infection with the cultured vesicles.
abstract: Alveolar echinococcosis (AE) is a lethal zoonosis caused by the fox-tapeworm Echinococcus multilocularis. The disease is difficult to treat and an effective therapeutic drug is urgently needed. Reliable models are essential for drug development. In this study, we developed both in vitro and in vivo models of larval E. multilocularis.
  
  
  
  The protoscoleces (PSC) of E. multilocularis from jirds were successfully cultured in a modified RPMI1640 based medium containing 25 % (v/v) fetal bovine serum (FBS). After 100 days of culture, PSC developed to larval vesicles (small unilocular cysts) and the fast growing vesicles produced PSC in brood capsules. In addition, mice were intraperitoneally injected with 30 cultured small vesicles and 100 % of the mice had resulting metacestode masses.
  
  
  
  Larval protoscoleces and vesicles of E. multilocularis grow healthily in vitro in the RPMI1640 based medium containing 25 % FBS. Echinococcus multilocularis in vitro and in vivo models provide a valuable platform for investigating the biology of the parasite and screening effective therapeutic drugs against AE.
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China.
authors.forename: Baoping
authors.initials: B
authors.lastname: Guo
authors.affiliation: Veterinary Research Institute, Xinjiang Academy of Animal Science, Urumqi, Xinjiang, 830000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Veterinary Research Institute, Xinjiang Academy of Animal Science, Urumqi, Xinjiang, 830000, China.
authors.forename: Zhuangzhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Molecular Parasitology Laboratory, Infectious Diseases Division, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia.
authors.forename: Donald P
authors.initials: DP
authors.lastname: McManus
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China. dr.haowen@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Wen
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China. wenbaozhang2013@163.com.
authors.forename: Wenbao
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D022162:Cytoplasmic Vesicles / Q000469:parasitology*; D004443:Echinococcosis / Q000469:parasitology / Q000662:veterinary*; D048210:Echinococcus multilocularis / Q000254:growth & development*; D005260:Female; D005849:Gerbillinae / Q000469:parasitology; D007814:Larva / Q000254:growth & development; D051379:Mice; D008807:Mice, Inbred BALB C; D012376:Rodent Diseases / Q000469:parasitology*
keywords: Animal model; Echinococcus multilocularis; In vitro culture; Protoscoleces; Vesicles