title: Overexpression of Δ12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids.
abstract: Under nutrient-limited conditions, the red yeast Rhodosporidium toruloides can accumulate neutral lipids, of which the compositional fatty acids are mainly saturated and mono-unsaturated ones with 16 or 18 carbon atoms. To improve the linoleic acid content in the lipids, we enabled galactose-inducible expression of the gene encoding Δ12-fatty acid desaturase (FADS) from Mortierella alpina or Fusarium verticillioides by integration of the corresponding expression cassettes into the genome of R. toruloides haploid and diploid strains. The relative linoleic acid content increased up to fivefold and the final linoleic acid titer reached 1.3 g/L under flask culture conditions. Our results suggested that R. toruloides may be further explored as cell factory for production of high-valued lipids and other fatty acid derivatives as bio-based chemicals and fuels.
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China. zsfjxy@dicp.ac.cn.
authors.forename: Sufang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Evonik Degussa (China) Co. Ltd., Shanghai Branch, Shanghai, 201108, China.
authors.forename: Markus
authors.initials: M
authors.lastname: Pötter
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Sun
authors.affiliation: Evonik Degussa (China) Co. Ltd., Shanghai Branch, Shanghai, 201108, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Yang
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Jiao
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China. zhaozb@dicp.ac.cn.
authors.forename: Zongbao K
authors.initials: ZK
authors.lastname: Zhao
mesh_terms: D001487:Basidiomycota / Q000235:genetics / Q000378:metabolism*; D004271:DNA, Fungal / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005670:Fusarium / Q000201:enzymology; D016681:Genome, Fungal; D019787:Linoleic Acid / Q000096:biosynthesis*; D010957:Plasmids / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D019787:Linoleic Acid; D044943:Fatty Acid Desaturases; C040448:delta-12 fatty acid desaturase
keywords: Genetic engineering; Linoleic acid; Microbial lipids; Rhodosporidium toruloides; Δ12-fatty acid desaturase