title: Fluorescent vinblastine probes for live cell imaging.
abstract: Herein we describe the synthesis of several fluorescent analogues of the clinically approved microtubule destabilizing agent vinblastine. The evaluated probes are the most potent described and provides the first example of uptake, distribution and live cell imaging using this well known antimitotic agent.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, USA. rweissleder@mgh.harvard.edu.
authors.forename: Labros G
authors.initials: LG
authors.lastname: Meimetis
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, USA. rweissleder@mgh.harvard.edu.
authors.forename: Randy J
authors.initials: RJ
authors.lastname: Giedt
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, USA. rweissleder@mgh.harvard.edu.
authors.forename: Hannes
authors.initials: H
authors.lastname: Mikula
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, USA. rweissleder@mgh.harvard.edu.
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Carlson
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, USA. rweissleder@mgh.harvard.edu.
authors.forename: Rainer H
authors.initials: RH
authors.lastname: Kohler
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, USA. rweissleder@mgh.harvard.edu.
authors.forename: David B
authors.initials: DB
authors.lastname: Pirovich
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, USA. rweissleder@mgh.harvard.edu and Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA.
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D017433:Protein Structure, Secondary; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism; D014747:Vinblastine / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D014404:Tubulin; D014747:Vinblastine