title: Development and optimization of a multiplex lateral flow immunoassay for the simultaneous determination of three mycotoxins in corn, rice and peanut.
abstract: A multiplex lateral flow immunoassay (LFA) is developed for the simultaneous on-site determination of three mycotoxins (aflatoxin B1, zearalenone and ochratoxin A) in corn, rice and peanut. By systematically optimizing the preparation of antibody-gold nanoparticle conjugates, the size of gold nanoparticle and the position of capture antigen, the developed LFA can obtain a visual detection limit of 10μg/kg for aflatoxin B1, 50μg/kg for zearalenone and 15μg/kg for ochratoxin A. For quantitative analysis, the limits of detection were 0.10-0.13μg/kg for aflatoxin B1, 0.42-0.46μg/kg for zearalenone, and 0.19-0.24μg/kg for ochratoxin A, which were far below the regulatory limits set by the European Commission. At the spiked concentrations of 0.5-10.0μg/kg, the mean recoveries of the three mycotoxins ranged from 86.2 to 114.5% with coefficients of variation less than 16.7%. These results demonstrated that the developed immunoassay can be used for routine monitoring of mycotoxin contamination.
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China. Electronic address: yqchen@cau.edu.cn.
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: National Key Laboratory of Bio-membrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Science, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Han
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Niu
authors.affiliation: China Institute of Veterinary Drug Control, Beijing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL, USA.
authors.forename: Lidong
authors.initials: L
authors.lastname: He
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China. Electronic address: zhangliying01@sina.com.
authors.forename: Liying
authors.initials: L
authors.lastname: Zhang
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis; D010367:Arachis / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis; D012275:Oryza / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*; D015025:Zearalenone / Q000032:analysis
chemical_list: D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A; D015025:Zearalenone; D006046:Gold; D016604:Aflatoxin B1
keywords: Aflatoxin B(1); Lateral flow immunoassay; Multiplex; Ochratoxin A; Zearalenone