title: Elaboration and Physicochemical Characterization of Niosome-Based Nioplexes for Gene Delivery Purposes.
abstract: Niosome formulations for gene delivery purposes are based on nonionic surfactants, helper lipids, and cationic lipids that interact electrostatically with negatively charged DNA molecules to form the so-called nioplexes. Niosomes are elaborated by different techniques, such as solvent emulsion-evaporation, thin film hydration, hand-shaking, dissolvent injection, and microfluidization method, among many others. In this chapter, we have described some protocols for the elaboration of niosomes and nioplexes and their physicochemical characterization that guarantees the quality criteria of the formulation in terms of size, morphology, ζ-potential, and stability.
authors.affiliation: NanoBioCel Group, University of the Basque Country (UPV-EHU), Vitoria-Gasteiz, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
authors.forename: Edilberto
authors.initials: E
authors.lastname: Ojeda
authors.affiliation: NanoBioCel Group, University of the Basque Country (UPV-EHU), Vitoria-Gasteiz, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
authors.forename: Mireia
authors.initials: M
authors.lastname: Agirre
authors.affiliation: NanoBioCel Group, University of the Basque Country (UPV-EHU), Vitoria-Gasteiz, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
authors.forename: Ilia
authors.initials: I
authors.lastname: Villate-Beitia
authors.affiliation: NanoBioCel Group, University of the Basque Country (UPV-EHU), Vitoria-Gasteiz, Spain.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Mashal
authors.affiliation: NanoBioCel Group, University of the Basque Country (UPV-EHU), Vitoria-Gasteiz, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Puras
authors.affiliation: NanoBioCel Group, University of the Basque Country (UPV-EHU), Vitoria-Gasteiz, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
authors.forename: Jon
authors.initials: J
authors.lastname: Zarate
authors.affiliation: NanoBioCel Group, University of the Basque Country (UPV-EHU), Vitoria-Gasteiz, Spain. joseluis.pedraz@ehu.es.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain. joseluis.pedraz@ehu.es.|Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of the Basque Country, 01006, Vitoria-Gasteiz, Spain. joseluis.pedraz@ehu.es.
authors.forename: Jose L
authors.initials: JL
authors.lastname: Pedraz
mesh_terms: D002412:Cations / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018014:Gene Transfer Techniques; D008081:Liposomes / Q000138:chemical synthesis* / Q000737:chemistry; D010316:Particle Size; D013501:Surface-Active Agents / Q000737:chemistry; D014162:Transfection
chemical_list: D002412:Cations; D008081:Liposomes; D013501:Surface-Active Agents; D004247:DNA
keywords: Cationic lipid; Gene delivery; Niosomes; Nonionic surfactant; Nonviral vector; Transfection