title: Ameliorating effects of 1,8-cineole on LPS-induced human umbilical vein endothelial cell injury by suppressing NF-κB signaling in vitro.
abstract: 1,8-Cineole (also known as eucalyptol) is a monoterpene that occurs naturally in many aromatic plants, 1,8-cineole has been reported to ameliorate dysfunction of endothelial cells. However, the mechanism of action of 1,8-cineole is incompletely understood. We investigated the protective effect of 1,8-cineole on lipopolysaccharide (LPS)-induced human umbilical vein endothelial cell (HUVEC) injury and the underlying mechanisms. HUVECs were preincubated with 1,8-cineole for 1.5h, then exposed to LPS for 12h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase leakage assays showed 1,8-cineole reduced LPS-induced HUVEC injury significantly. Results from enzyme linked immunosorbent assays revealed that 1,8-cineole suppressed LPS-induced secretion of interleukin-6 and interleukin-8, and recovered nitric oxide to normal levels. 1,8-Cineole decreased phosphorylation of nuclear factor-kappa B (NF-κB) p65 and expression of inducible nitric oxide synthase, and simultaneously improved protein levels of endothelial nitric oxide synthase. Immunofluorescence confirmed 1,8-cineole moderates nuclear translocation of NF-κB. These results suggest that 1,8-cineole ameliorates HUVEC dysfunction significantly, and that this effect at least involves NF-κB suppression.
authors.affiliation: The Key Lab of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China.
authors.forename: Kegang
authors.initials: K
authors.lastname: Linghu
authors.affiliation: The Key Lab of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Lin
authors.affiliation: The Key Lab of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: The Key Lab of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China.
authors.forename: Yini
authors.initials: Y
authors.lastname: Xu
authors.affiliation: The Key Lab of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Key Lab of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Tao
authors.affiliation: The Key Lab of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China; Department of Pharmacology of Materia Medica, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The Key Lab of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China; Department of Pharmacology of Materia Medica, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China. Electronic address: shenxiangchun@126.com.
authors.forename: Xiangchun
authors.initials: X
authors.lastname: Shen
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003511:Cyclohexanols / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D000077591:Eucalyptol; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D039821:Monoterpenes / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D003511:Cyclohexanols; D016207:Cytokines; D008070:Lipopolysaccharides; D039821:Monoterpenes; D016328:NF-kappa B; D051996:Transcription Factor RelA; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D000077591:Eucalyptol
keywords: 1,8-Cineole; Human umbilical vein endothelial cell (HUVEC); Lipopolysaccharide (LPS); Nitric oxide (NO); Nuclear transcription factor-kappa B (NF-κB)