title: A comparison study of macrocyclic hosts functionalized reduced graphene oxide for electrochemical recognition of tadalafil.
abstract: The present work described the comparison of β-cyclodextrin (β-CD) and p-sulfonated calix[6]arene (SCX6) functionalized reduced graphene oxide (RGO) for recognition of tadalafil. In this study, tadalafil and two macrocycles (β-CD and SCX6) were selected as the guest and host molecules, respectively. The inclusion complexes of β-CD/tadalafil and SCX6/tadalafil were studied by UV spectroscopy and molecular simulation calculations, proving the higher supermolecular recognition capability of SCX6 than β-CD towards tadalafil. The β-CD@RGO and SCX6@RGO composites were prepared by a wet-chemical route. The obtained composites were characterized by Fourier transform infrared spectrometry, thermogravimetric analysis, atomic force microscopy, and zeta potential. The SCX6@RGO showed a higher electrochemical response than β-CD@RGO, which was caused by the higher recognition capability of SCX6 than β-CD. By combining the merits of SCX6 and the RGO, a sensitive electrochemical sensing platform was developed based on the SCX6@RGO nanohybrids. A linear response range of 0.1-50 μM and 50-1000 μM for tadalafil with a low detection limit of 0.045 μM (S/N=3) was obtained by using this method. The constructed sensing platform was successfully used to determine tadalafil in herbal sexual health products and spiked human serum samples, suggesting its promising analytical applications for the trace level determination of tadalafil.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China.
authors.forename: Yucong
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ran
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China.
authors.forename: Hanzhang
authors.initials: H
authors.lastname: Ye
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China.
authors.forename: Genfu
authors.initials: G
authors.lastname: Zhao
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China.
authors.forename: Yanqiong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China. Electronic address: lcppp1974@sina.com.
authors.forename: Can-Peng
authors.initials: CP
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D047250:Calixarenes / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D013450:Sulfones / Q000737:chemistry; D000068581:Tadalafil / Q000032:analysis / Q000097:blood*; D014665:Vasodilator Agents / Q000032:analysis / Q000097:blood*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D010087:Oxides; D010636:Phenols; D013450:Sulfones; D014665:Vasodilator Agents; D047392:beta-Cyclodextrins; C476540:calix(6)arene; D047250:Calixarenes; D000068581:Tadalafil; D006108:Graphite; C031215:betadex
keywords: Inclusion complex; Molecular recognition; Reduced graphene oxide; p-Sulfonated calix[6]arene; β-cyclodextrin