title: Two Consecutive Days of Crossfit Training Affects Pro and Anti-inflammatory Cytokines and Osteoprotegerin without Impairments in Muscle Power.
abstract: The aim of this study was to investigate the effects of two consecutive Crossfit® training sessions (24 h apart) designed to enhance work-capacity that involved both cardiovascular and muscular exercises on cytokines, muscle power, blood lactate and glucose. Nine male members of the CrossFit® community (age 26.7 ± 6.6 years; body mass 78.8 ± 13.2 kg; body fat 13.5 ± 6.2%; training experience 2.5 ± 1.2 years) completed two experimental protocols (24 h apart): (1) strength and power exercises, (2) gymnastic movements, and (3) metabolic conditioning as follows: 10 min of as many rounds as possible (AMRAP) of 30 double-unders and 15 power snatches (34 kg). The same sequence as repeated on session 2 with the following metabolic conditioning: 12 min AMRAP of: row 250 m and 25 target burpees. Serum interleukin-6 (IL-6), IL-10, and osteoprotegerin were measured before, immediately post and 24 h after workout of the day (WOD) 1, immediately post, 24 and 48 h after WOD 2. Peak and mean power were obtained for each repetition (back squat with 50% of 1 repetition maximum) using a linear position transducer measured before, immediately post and 24 h after WOD 1, immediately post and 24 h after WOD 2. Blood lactate and glucose were measured pre and immediately post WOD 1 and 2. Although both sessions of exercise elicited an significant increase in blood lactate (1.20 ± 0.41 to 11.84 ± 1.34 vs. 0.94 ± 0.34 to 9.05 ± 2.56 mmol/l) and glucose concentration (81.59 ± 10.27 to 114.99 ± 12.52 vs. 69.47 ± 6.97 to 89.95 ± 19.26 mg/dL), WOD 1 induced a significantly greater increase than WOD 2 (p ≤ 0.05). The training sessions elicited significant changes (p ≤ 0.05) in IL-6, IL-10 and osteoprotegerin concentration over time. IL-6 displayed an increase immediately after training WOD 1 [197 ± 109%] (p = 0.009) and 2 [99 ± 58%] (p = 0.045). IL-10 displayed an increase immediately after only WOD 1 [44 ± 52%] (p = 0.046), and decreased 24 and 48 h following WOD 2 (~40%; p = 0.018) as compared to pre-exercise values. Osteoprotegerin displayed a decrease 48 h following WOD 2 (~25%; p = 0.018) as compared with pre intervention. In conclusion, two consecutive Crossfit® training sessions increase pro/anti-inflammatory cytokines with no interference on muscle performance in the recovery period.
authors.affiliation: Graduation Program on Physical Education, Catholic University of Brasilia Brasilia, Brazil.
authors.forename: Ramires A
authors.initials: RA
authors.lastname: Tibana
authors.affiliation: Graduation Program on Physical Education, Catholic University of Brasilia Brasilia, Brazil.
authors.forename: Leonardo M
authors.initials: LM
authors.lastname: de Almeida
authors.affiliation: Laboratory of Exercise Physiology, Faculty Estacio of Vitoria Vitoria, Brazil.
authors.forename: Nuno M
authors.initials: NM
authors.lastname: Frade de Sousa
authors.affiliation: Graduation Program on Physical Education, Catholic University of Brasilia Brasilia, Brazil.
authors.forename: Dahan da Cunha
authors.initials: Dda C
authors.lastname: Nascimento
authors.affiliation: Graduation Program on Physical Education, Catholic University of Brasilia Brasilia, Brazil.
authors.forename: Ivo V de Sousa
authors.initials: IV
authors.lastname: Neto
authors.affiliation: Graduation Program on Physical Education, Catholic University of Brasilia Brasilia, Brazil.
authors.forename: Jeeser A
authors.initials: JA
authors.lastname: de Almeida
authors.affiliation: Medical Sciences, Faculty of Medicine, University of Brasilia Brasilia, Brazil.
authors.forename: Vinicius C
authors.initials: VC
authors.lastname: de Souza
authors.affiliation: Graduation Program on Physical Education, Catholic University of Brasilia Brasilia, Brazil.
authors.forename: Maria de Fátima T P L
authors.initials: Mde F
authors.lastname: Lopes
authors.affiliation: Medical Sciences, Faculty of Medicine, University of Brasilia Brasilia, Brazil.
authors.forename: Otávio de Tolêdo
authors.initials: Ode T
authors.lastname: Nobrega
authors.affiliation: Graduation Program on Physical Education, Catholic University of BrasiliaBrasilia, Brazil; UDF-Centro UniversitárioBrasília, Brazil.
authors.forename: Denis C L
authors.initials: DC
authors.lastname: Vieira
authors.affiliation: Department of Kinesiology and Nutrition Sciences, University of Nevada Las Vegas, NV, USA.
authors.forename: James W
authors.initials: JW
authors.lastname: Navalta
authors.affiliation: Graduation Program on Physical Education, Catholic University of Brasilia Brasilia, Brazil.
authors.forename: Jonato
authors.initials: J
authors.lastname: Prestes
keywords: extreme condition; inflammatory response; muscle power; weight training