title: A novel leucine-rich repeat protein, CaLRR51, acts as a positive regulator in the response of pepper to Ralstonia solanacearum infection.
abstract: The leucine-rich repeat (LRR) proteins play important roles in the recognition of corresponding ligands and signal transduction networks in plant defence responses. Herein, a novel LRR protein from Capsicum annuum, CaLRR51, was identified and characterized. It was localized to the plasma membrane and transcriptionally up-regulated by Ralstonia solanacearum infection (RSI), as well as the exogenous application of salicylic acid (SA), jasmonic acid (JA) and ethephon (ETH). Virus-induced gene silencing of CaLRR51 significantly increased the susceptibility of pepper to RSI. By contrast, transient overexpression of CaLRR51 in pepper plants activated hypersensitive response (HR)-like cell death, and up-regulated the defence-related marker genes, including PO2, HIR1, PR1, DEF1 and ACO1. Moreover, ectopic overexpression of CaLRR51 in transgenic tobacco plants significantly enhanced the resistance to RSI. Transcriptional expression of the corresponding defence-related marker genes in transgenic tobacco plants was also found to be enhanced by the overexpression of CaLRR51, which was potentiated by RSI. These loss- and gain-of-function assays suggest that CaLRR51 acts as a positive regulator in the response of pepper to RSI. In addition, the putative signal peptide and transmembrane region were found to be required for plasma membrane targeting of CaLRR51, which is indispensable for the role of CaLRR51 in plant immunity.
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cheng
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Zhuoli
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Hanyang
authors.initials: H
authors.lastname: Cai
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Chuanqing
authors.initials: C
authors.lastname: Wang
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Yueping
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shen
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Shaoliang
authors.initials: S
authors.lastname: Mou
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Ailian
authors.initials: A
authors.lastname: Qiu
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Deyi
authors.initials: D
authors.lastname: Guan
authors.affiliation: National Education Ministry, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Shuilin
authors.initials: S
authors.lastname: He
mesh_terms: D000595:Amino Acid Sequence; D002212:Capsicum / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D016923:Cell Death / Q000187:drug effects; D003001:Cloning, Molecular; D060467:Disease Resistance / Q000235:genetics; D017353:Gene Deletion; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing / Q000187:drug effects; D000090263:Leucine-Rich Repeat Proteins; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism*; D010942:Plant Viruses / Q000502:physiology; D030821:Plants, Genetically Modified; D000072417:Protein Domains; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043368:Ralstonia solanacearum / Q000187:drug effects / Q000472:pathogenicity*; D020539:Sequence Analysis, Protein; D013347:Subcellular Fractions / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000090263:Leucine-Rich Repeat Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011506:Proteins; D012333:RNA, Messenger
keywords: Capsicum annuum; Ralstonia solanacearum; leucine-rich repeat (LRR) protein; plant defence response