title: Discrimination of rat Brunner's gland carbohydrate antigens by site-specific monoclonal antibodies.
abstract: Mucus produced and secreted by gastrointestinal mucosa contains various types of mucins equipped with unique sugar chains considered to play critical roles in protecting mucous membranes; therefore, the identification and verification of mucin sugar chains is important for understanding the underlying mechanisms. In our previous work, we generated three monoclonal antibodies (mAbs), RGM22, RGM26, and RGM42, which recognize sugar chains in rat gastric mucin. Here, we immunohistochemically analyzed the rat gastrointestinal mucosa and found that the antigens recognized by RGM22 and RGM42 were expressed in the rat antrum and Brunner's glands, whereas that recognized by RGM26 was detected in the antrum, but rarely in Brunner's glands. We found that these antibodies reacted with porcine gastric mucin-derived oligosaccharides bearing a common structure: GalNAcα1-3(Fucα1-2)Galβ1-4GlcNAcβ1-6GalNAc-ol. Moreover, epitope analysis revealed that RGM42 and RGM22 recognized α-linked GalNAc and GalNAcα1-3Gal, respectively, on the GalNAcα1-3(Fucα1-2)Gal structure, whereas RGM26 was specific for GalNAcα1-3(Fucα1-2)Gal. These results indicate that rat Brunner's glands express specific antigens bearing GalNAcα1-3Gal that are recognized by RGM22 and RGM42. Thus, RGM22, RGM26, and RGM42 with their unique antigen specificities could be useful tools for investigation of oligosaccharide diversity among mucins.
authors.affiliation: Isehara Research Laboratory, Kanto Chemical Co., Inc., 21 Suzukawa, Isehara, Kanagawa 259-1146, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Chimuro
authors.affiliation: Isehara Research Laboratory, Kanto Chemical Co., Inc., 21 Suzukawa, Isehara, Kanagawa 259-1146, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kuroyama
authors.affiliation: Department of Biochemistry, Kitasato University School of Medicine, 1-15-1, Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Goso
authors.affiliation: Kitasato Junior College of Health and Hygienic Sciences, 500, Kurotsuchishinden, Minami-uonuma, Niigata 949-7241, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishihara
authors.affiliation: Department of Applied Bioscience, Kanagawa Institute of Technology, 1030, Shimo-ogino, Atsugi, Kanagawa 243-0292, Japan. Electronic address: kurihara@bio.kanagawa-it.ac.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kurihara
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002011:Brunner Glands / Q000276:immunology*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D000939:Epitopes / Q000378:metabolism; D005752:Gastric Mucins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology; D051381:Rats; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D000939:Epitopes; D005752:Gastric Mucins
keywords: Carbohydrate antigen; Epitope; Gastrointestinal mucin; Monoclonal antibody; Rat Brunner's gland