title: Targeted Quantitative Screening of Chromosome 18 Encoded Proteome in Plasma Samples of Astronaut Candidates.
abstract: This work was aimed at estimating the concentrations of proteins encoded by human chromosome 18 (Chr 18) in plasma samples of 54 healthy male volunteers (aged 20-47). These young persons have been certified by the medical evaluation board as healthy subjects ready for space flight training. Over 260 stable isotope-labeled peptide standards (SIS) were synthesized to perform the measurements of proteins encoded by Chr 18. Selected reaction monitoring (SRM) with SIS allowed an estimate of the levels of 84 of 276 proteins encoded by Chr 18. These proteins were quantified in whole and depleted plasma samples. Concentration of the proteins detected varied from 10-6M (transthyretin, P02766) to 10-11M (P4-ATPase, O43861). A minor part of the proteins (mostly representing intracellular proteins) was characterized by extremely high inter individual variations. The results provide a background for studies of a potential biomarker in plasma among proteins encoded by Chr 18. The SRM raw data are available in ProteomeXchange repository (PXD004374).
authors.affiliation: Institute of Biomedical Chemistry , 119121, Moscow, Russia.
authors.forename: Artur T
authors.initials: AT
authors.lastname: Kopylov
authors.affiliation: Institute of Biomedical Chemistry , 119121, Moscow, Russia.
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Ilgisonis
authors.affiliation: Institute of Biomedical Chemistry , 119121, Moscow, Russia.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Moysa
authors.affiliation: Institute of Biomedical Chemistry , 119121, Moscow, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Tikhonova
authors.affiliation: Institute of Biomedical Chemistry , 119121, Moscow, Russia.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Zavialova
authors.affiliation: Institute of Biomedical Chemistry , 119121, Moscow, Russia.
authors.forename: Svetlana E
authors.initials: SE
authors.lastname: Novikova
authors.affiliation: Institute of Biomedical Chemistry , 119121, Moscow, Russia.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Lisitsa
authors.affiliation: Institute of Biomedical Chemistry , 119121, Moscow, Russia.
authors.forename: Elena A
authors.initials: EA
authors.lastname: Ponomarenko
authors.affiliation: Institute of Biomedical Chemistry , 119121, Moscow, Russia.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Moshkovskii
authors.affiliation: Institute of Medico-Biological Problems, Russian Academy of Sciences , 123007, Moscow, Russia.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Markin
authors.affiliation: Institute of Medico-Biological Problems, Russian Academy of Sciences , 123007, Moscow, Russia.
authors.forename: Anatoly I
authors.initials: AI
authors.lastname: Grigoriev
authors.affiliation: Institute of Biomedical Chemistry , 119121, Moscow, Russia.
authors.forename: Victor G
authors.initials: VG
authors.lastname: Zgoda
authors.affiliation: Institute of Biomedical Chemistry , 119121, Moscow, Russia.
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis; D000328:Adult; D018480:Astronauts; D002887:Chromosomes, Human, Pair 18; D064368:Healthy Volunteers; D006801:Humans; D008875:Middle Aged; D010949:Plasma / Q000737:chemistry*; D011228:Prealbumin / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D055815:Young Adult
chemical_list: D011228:Prealbumin; D020543:Proteome; D000251:Adenosine Triphosphatases
keywords: C-HPP; human chromosome 18 (Chr 18); plasma proteome; selected reaction monitoring (SRM); targeted proteomics