title: Phenotypic screening with primary neurons to identify drug targets for regeneration and degeneration.
abstract: High-throughput, target-based screening techniques have been utilized extensively for drug discovery in the past several decades. However, the need for more predictive in vitro models of in vivo disease states has generated a shift in strategy towards phenotype-based screens. Phenotype based screens are particularly valuable in studying complex conditions such as CNS injury and degenerative disease, as many factors can contribute to a specific cellular response. In this review, we will discuss different screening frameworks and their relative utility in examining mechanisms of neurodegeneration and axon regrowth, particularly in cell-based in vitro disease models.
authors.affiliation: The Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami, 1400 NW 12th Ave, Miami, FL 33136, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Cooper
authors.affiliation: The Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami, 1400 NW 12th Ave, Miami, FL 33136, USA.
authors.forename: Giulia
authors.initials: G
authors.lastname: Zunino
authors.affiliation: The Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami, 1400 NW 12th Ave, Miami, FL 33136, USA.|Center for Computational Science, University of Miami, 1400 NW 12th Ave, Miami, FL 33136, USA.|Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, 1400 NW 12th Ave, Miami, FL 33136, USA.
authors.forename: John L
authors.initials: JL
authors.lastname: Bixby
authors.affiliation: The Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami, 1400 NW 12th Ave, Miami, FL 33136, USA.|Center for Computational Science, University of Miami, 1400 NW 12th Ave, Miami, FL 33136, USA.
authors.forename: Vance P
authors.initials: VP
authors.lastname: Lemmon
mesh_terms: D000818:Animals; D055808:Drug Discovery; D004353:Drug Evaluation, Preclinical; D006801:Humans; D009410:Nerve Degeneration / Q000188:drug therapy*; D009416:Nerve Regeneration / Q000187:drug effects*; D010641:Phenotype
keywords: Axon degeneration; Axon growth; Axon regeneration; High content analysis; High throughput screening; Phenotypic analysis