title: Polymorphisms of FLII implicate gene expressions and growth traits in Chinese cattle.
abstract: Flightless-1 (FLII) is essential for early embryogenesis, structural organization of indirect flight muscle and can inhibit adipocyte differentiation. We therefore aimed to identify common variations in FLII gene and to investigate their effects on cattle growth traits. By DNA sequencing and forced PCR-RFLP methods, we evaluated two synonymous mutations (rs41910826 and rs444484913) and one intron mutation (rs522737248) in four Chinese domestic breeds (n = 628). Association analysis indicated that these SNPs were associated with growth traits and gene expressions (P < 0.05). At rs41910826, individuals with TT and/or CT genotypes had some better body sizes in Jiaxian, Nanyang, and Qinchuan breeds. Consistently, among adult Qinchuan cattle muscles, quantitative real-time PCR study witnessed considerable increases of mRNA level of FLII in cattle with CT genotype. For rs444484913, TT and/or TC genotypes were significantly associated with increased body traits of Qinchuan cattle while the qPCR data showed that the TT genotype was more conducive to FLII expression in fetal muscle. At rs522737248, performances of cattle with AA genotype showed compelling superior merits in all four breeds, and allele A had an increasing tendency for the mRNA expression of PPARγ in adult adipose and FLII in fetal muscle. These findings strongly demonstrate that the three SNPs of FLII gene could be utilized as molecular markers for future assisted selection in cattle breeding program.
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, 712100, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, 712100, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, 712100, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, 712100, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, 712100, China.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, 712100, China.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.affiliation: Bureau of Animal Husbandry of Biyang County, Biyang, Henan, 463700, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Qu
authors.affiliation: Bureau of Animal Husbandry of Biyang County, Biyang, Henan, 463700, China.
authors.forename: Xingshan
authors.initials: X
authors.lastname: Qi
authors.affiliation: Animal Health Supervision in Henan Province, Zhengzhou, Henan, 450008, China. Electronic address: postbai@sohu.com.
authors.forename: Yueyu
authors.initials: Y
authors.lastname: Bai
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, 712100, China. Electronic address: chenhong1212@263.net.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D004252:DNA Mutational Analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005828:Genetics, Population; D060005:Genotyping Techniques; D006239:Haplotypes / Q000235:genetics; D015810:Linkage Disequilibrium / Q000235:genetics; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D019655:Quantitative Trait, Heritable
chemical_list: D008840:Microfilament Proteins
keywords: Association analysis; Cattle; FLII; Gene expression; Growth trait; Polymorphism