title: Reduction Levels and the Effects of High-Molecular-Weight Adiponectin via AMPK/eNOS in Chinese Type 2 Diabetes.
abstract: Aim:This study was to investigate the change of high-molecula-weight (HMW) adiponectin (APN) isoform, the association between type 2 diabetes mellitus (T2DM) and HMW APN isoform, the variation of Disulfide-bond A oxidoreductase-like protein (DsbA-L), the effect of HMW APN isoform on AMP-dependent protein kinase (AMPK) and endothelial nitric oxide synthase (eNOS) in Chinese T2DM.Method:169 patients aged at (48.7±9.4) years and 107 healthy control subjects aged at (42.6±7.8) years took part in this study. Anthropometric measures of the characters were assayed and different APN isoforms, DsbA-L, AMPK and eNOS levels were determined.Results:Ln(sRAGE) and Ln(Adiponectin) were significantly lower and significantly higher for the other characteristics in T2DM. Ln(Adiponectin) was negatively and significantly correlated with WHR, Ln(triglycerides), fasting plasma glucose, HbA1c (%) in control subjects and T2DM patients. Plasma and adipose tissue total APN and HMW APN were significantly reduced in newly diagnosed T2DM patients. DsbA-L was markedly down-regulated in diabetic adipose tissue. HMW APN caused significant decreases in AMPK and eNOS phosphorylation levels of human umbilical vein endothelial cells (HUVECs).Conclusions:Our results demonstrated that total APN levels was closely related to the risk of T2DM and HMW APN reduction was involved in the diabetic vascular AMPK/eNOS signal pathway. The findings will provide insight into novel therapeutic approaches for reducing the elevated cardiovascular risk associated with T2DM.
authors.affiliation: Department of Cardiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: K
authors.initials: K
authors.lastname: Lian
authors.affiliation: Department of Cardiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: X
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Endocrinology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Health Statistics, Fourth Military Medical University, Xi'an, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Department of Cardiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: C
authors.initials: C
authors.lastname: Xia
authors.affiliation: Department of Cardiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: R
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: F
authors.initials: F
authors.lastname: Yi
authors.affiliation: Department of Cardiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: Health Examination Center, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Plastic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: S
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Cardiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Ji
authors.affiliation: Department of Cardiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
authors.forename: L
authors.initials: L
authors.lastname: Tao
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000097:blood*; D052242:Adiponectin / Q000097:blood*; D000328:Adult; D002681:China; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D005982:Glutathione Transferase / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000097:blood*
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; C487896:GSTK1 protein, human; D005982:Glutathione Transferase; D055372:AMP-Activated Protein Kinases