title: The bridge between thin layer chromatography-mass spectrometry and high-performance liquid chromatography-mass spectrometry: The realization of liquid thin layer chromatography-mass spectrometry (LTLC-MS).
abstract: The combination of thin layer chromatography (TLC) and mass spectrometry (MS) has been studied for decades, but for most cases MS detection is done after TLC separation is finished. Here, an online simultaneous TLC-MS analysis system, liquid thin layer chromatography-mass spectrometry (LTLC-MS), is developed which successfully synchronize TLC separation process and MS detection process like GC-MS and HPLC-MS do. And there's no need to use specially designed TLC, just regular TLC plates are enough. LTLC-MS method is composed of a newly developed ambient ionization method, glow discharge-matrix assisted infrared desorption ionization (GD-MAIRDI), and forced-flow TLC (FFTLC) technique, which guarantees the MS detection process does not disturb the TLC separation process throughout the whole analysis. The whole LTLC-MS analysis only need two steps and less than 15min. Mixtures as well as the two main components of a pain relief pills have been successfully analyzed by LTLC-MS. This proof of concept study opens up new possibilities of combining TLC with MS, and will further broaden the application abilities of TLC.
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing 100190, China; Beijing National Laboratory for Molecular Sciences, Beijing 100190, China.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing 100190, China; Beijing National Laboratory for Molecular Sciences, Beijing 100190, China.
authors.forename: Jianing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing 100190, China; Beijing National Laboratory for Molecular Sciences, Beijing 100190, China.
authors.forename: Lingpeng
authors.initials: L
authors.lastname: Zhan
authors.affiliation: Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University, West Lafayette, IN 47907, United States.
authors.forename: Michael
authors.initials: M
authors.lastname: Wleklinski
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing 100190, China; Beijing National Laboratory for Molecular Sciences, Beijing 100190, China.
authors.forename: Jiyun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing 100190, China; Beijing National Laboratory for Molecular Sciences, Beijing 100190, China.
authors.forename: Caiqiao
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing 100190, China; Beijing National Laboratory for Molecular Sciences, Beijing 100190, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Chemistry, Biology and Materials Science, East China University of Technology, Nanchang, 330013, China.
authors.forename: Yueming
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing 100190, China; Beijing National Laboratory for Molecular Sciences, Beijing 100190, China; National Center for Mass Spectrometry in Beijing, Beijing 100190, China. Electronic address: znie@iccas.ac.cn.
authors.forename: Zongxiu
authors.initials: Z
authors.lastname: Nie
mesh_terms: D000632:Aminopyrine / Q000032:analysis; D000700:Analgesics / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods*; D010615:Phenacetin / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013607:Tablets / Q000737:chemistry
chemical_list: D000700:Analgesics; D013607:Tablets; D000632:Aminopyrine; D010615:Phenacetin
keywords: Ambient ionization; Glow discharge ionization; Interface of MS and TLC; On-line separation and detection; Pencil mark; Reaction monitoring