title: Pentobarbital modifies the lipid raft-protein interaction: A first clue about the anesthesia mechanism on NMDA and GABAAreceptors.
abstract: Recent studies have shown that anesthetic agents alter the physical properties of lipid rafts on model membranes. However, if this destabilization occurs in brain membranes, altering the lipid raft-protein interaction, remains unknown. We analyzed the effects produced by pentobarbital (PB) on brain plasma membranes and lipid rafts in vivo. We characterized for the first time the thermotropic behavior of plasma membranes, synaptosomes, and lipid rafts from rat brain. We found that the transition temperature from the ordered gel to disordered liquid phase of lipids is close to physiological temperature. We then studied the effect of PB on protein composition of lipid rafts. Our results show a reduction of the total protein associated to rafts, with a higher reduction of the NMDAR compared to the GABAAreceptor. Both receptors are considered the main targets of PB. In general, our results suggest that lipid rafts could be plausible mediators in anesthetic action.
authors.affiliation: Cinvestav-Monterrey, PIIT, Apodaca, Nuevo León, 66600, Mexico.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Sierra-Valdez
authors.affiliation: Cinvestav-Monterrey, PIIT, Apodaca, Nuevo León, 66600, Mexico.
authors.forename: J C
authors.initials: JC
authors.lastname: Ruiz-Suárez
authors.affiliation: Departamento de Farmacología y Toxicología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico; Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Monterrey, Mexico. Electronic address: ilsedelint@hotmail.com.
authors.forename: Ilse
authors.initials: I
authors.lastname: Delint-Ramirez
mesh_terms: D000758:Anesthesia; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression; D006993:Hypnotics and Sedatives / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D021962:Membrane Microdomains / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D010424:Pentobarbital / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000096:biosynthesis / Q000235:genetics*; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D013574:Synaptosomes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D044366:Transition Temperature
chemical_list: D006993:Hypnotics and Sedatives; D011963:Receptors, GABA-A; D016194:Receptors, N-Methyl-D-Aspartate; D010424:Pentobarbital
keywords: Anesthesia; Calorimetry; Lipid membrane; Receptors