title: Inhibition of miR-21 by 3'/5'-Serinyl-Capped 2'-O-Methyl RNA Interspersed with 2'-O-(2-Amino-3-Methoxypropyl) Uridine Units.
abstract: miRNAs are highly conserved class of small ncRNAs whose involvement in human pathophysiologies is extensively investigated. MiR-21 is a well established oncogenic miRNA whose deregulation plays a significant role in onset and progression of cancer. The need of novel approaches to downregulate miR-21 is rapidly expanding. Potent inhibition of miR-21 is achieved by chemically modified 2'-O-methyl RNA oligonucleotide. The serinol capping at 3' and 5'ends and the interspersed 2'-O-(R-2-amino-3-methoxypropyl) uridine units enhance the nuclease resistance and efficacy of 2'-O-methyl RNA for the inhibition of miR-21. This represents a simple and novel modification for developing oligonucleotide-based therapeutics.
authors.affiliation: 1 Academy of Scientific and Innovative Research (AcSIR) , New Delhi, India .|2 CSIR-Institute of Genomics and Integrative Biology , Delhi, India .
authors.forename: Smita
authors.initials: S
authors.lastname: Nahar
authors.affiliation: 3 Department of Chemistry, Binghamton University, State University of New York , Binghamton, New York.
authors.forename: Venubabu
authors.initials: V
authors.lastname: Kotikam
authors.affiliation: 4 Organic Chemistry Division, CSIR-National Chemical Laboratory , Pune, India .
authors.forename: Vaijayanti A
authors.initials: VA
authors.lastname: Kumar
authors.affiliation: 1 Academy of Scientific and Innovative Research (AcSIR) , New Delhi, India .|2 CSIR-Institute of Genomics and Integrative Biology , Delhi, India .|4 Organic Chemistry Division, CSIR-National Chemical Laboratory , Pune, India .
authors.forename: Souvik
authors.initials: S
authors.lastname: Maiti
mesh_terms: D000070416:Antagomirs / Q000138:chemical synthesis / Q000235:genetics* / Q000378:metabolism; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D061986:MCF-7 Cells; D008745:Methylation; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012694:Serine / Q000737:chemistry*; D014529:Uridine / Q000031:analogs & derivatives*
chemical_list: D000070416:Antagomirs; D051017:Apoptosis Regulatory Proteins; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; C114487:PDCD4 protein, human; D016601:RNA-Binding Proteins; D012694:Serine; D008156:Luciferases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; D014529:Uridine
keywords: antagomirs; cancer; microRNA; noncoding RNA; oligonucleotides