title: Effect of tranilast in comparison with beclomethasone in chronic murine model of asthma.
abstract: The current investigation was taken to scrutinize the action of tranilast on the airway remodeling in chronic asthma in mice.
  
  
  
  Intraperitoneal injection of ovalbumin was applied to mice for sensitization and subsequent inhalation of 1% ovalbumin three times week for 10 weeks for challenge. Beclomethasone or tranilast were given daily for the 10 week challenge period. At the end of the study, lung weight index, total collagen content, bronchoalveolar lavage level of total and differential cell counts, interleukin-13, in addition to lung tissue nitrate/nitrite and transforming growth beta-1 were measured. Also, histological analysis was done.
  
  
  
  Asthmatic mice demonstrated apparent fibrotic changes. Significant airway fibrosis was demonstrated by hyperplasia of goblet cells and thickening of airway epithelium, increased content of lung collagen, lung and bronchoalveolar lavage of transforming growth factor beta-1 and interleukin-13 mutually accompanied by reduction in nitrate/nitrite generation.
  
  
  
  Beclomethasone influence on airway remodeling was mediated mainly via suppression of eosinophilic recruitment into the airways and reduction of interleukin-13 cytokine levels. Whereas, tranilast effects on airway remodeling was found to be mainly mediated via its inhibitory effect on transforming growth beta-1. Both beclomethasone and tranilast influence airway remodeling by different degrees and mechanisms.
authors.affiliation: a Department of Pharmacology & Toxicology , College of Pharmacy, Taibah University , El-Madinah El-Munawarah , Saudi Arabia.|b Department of Pharmacology & Toxicology, Faculty of Pharmacy , Mansoura University , Mansoura , Egypt.
authors.forename: Manar A
authors.initials: MA
authors.lastname: Nader
authors.affiliation: b Department of Pharmacology & Toxicology, Faculty of Pharmacy , Mansoura University , Mansoura , Egypt.
authors.forename: Nariman
authors.initials: N
authors.lastname: Gameil
authors.affiliation: b Department of Pharmacology & Toxicology, Faculty of Pharmacy , Mansoura University , Mansoura , Egypt.
authors.forename: Rania R
authors.initials: RR
authors.lastname: Abdelaziz
authors.affiliation: c Department of Clinical Pathology, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
authors.forename: Khaled R
authors.initials: KR
authors.lastname: Zalata
authors.affiliation: d Department of Pediatrics, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
authors.forename: Amal
authors.initials: A
authors.lastname: Osman
authors.affiliation: d Department of Pediatrics, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Zedan
authors.affiliation: c Department of Clinical Pathology, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
authors.forename: Nermin
authors.initials: N
authors.lastname: Abo-Elkheir
authors.affiliation: e Department of Pathology, Faculty of Medicine , Taibah University , El-Madinah El-Munawarah , Saudi Arabia.
authors.forename: Abeer Abdalla
authors.initials: AA
authors.lastname: Elsiddig
authors.affiliation: d Department of Pediatrics, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
authors.forename: Magdy
authors.initials: M
authors.lastname: Zedan
mesh_terms: D056151:Airway Remodeling / Q000187:drug effects*; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D018927:Anti-Asthmatic Agents / Q000494:pharmacology / Q000627:therapeutic use; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology; D001507:Beclomethasone / Q000494:pharmacology / Q000627:therapeutic use; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002908:Chronic Disease; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D018793:Interleukin-13 / Q000378:metabolism; D007958:Leukocyte Count; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D062367:ortho-Aminobenzoates / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D018926:Anti-Allergic Agents; D018927:Anti-Asthmatic Agents; D018793:Interleukin-13; C508961:Tgfb1 protein, mouse; D053773:Transforming Growth Factor beta1; D062367:ortho-Aminobenzoates; D009569:Nitric Oxide; D003094:Collagen; C012293:tranilast; D001507:Beclomethasone
keywords: beclomethasone; chronic asthma; inflammation; remodeling; tranilast