title: Toxicity of Hoechst 33342: implication in side population analysis.
abstract: In the current study, we discovered that Hoechst 33342 (H342) could produce significant toxicity to the human neuroblastoma SK-N-SH cells at concentrations typically used to identify side population (SP) cells. At 3.75 and 5 µg/ml, H342 induced cell death and apoptosis. Flow cytometry analysis revealed cell cycle arrest at S-phase. Our findings caution against using H342 for SP analysis without considering differential susceptibility of different cells to H342. Specifically, concentration of H342 needs to be adjusted carefully to minimize toxicity for each cell line.
authors.affiliation: Children's Hospital of Fudan University Department of Surgery Shanghai China.
authors.forename: J
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Anhui Proveince Children's Hospital Department of Surgery Anhui China.
authors.forename: S
authors.initials: S
authors.lastname: Qi
authors.affiliation: Children's Hospital of Fudan University Department of Surgery Shanghai China.
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Children's Hospital of Fudan University Department of Surgery Shanghai China xmxiao@shmu.edu.cn.
authors.forename: X
authors.initials: X
authors.lastname: Xiao
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001562:Benzimidazoles / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004273:DNA, Neoplasm / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D058985:Side-Population Cells / Q000166:cytology* / Q000187:drug effects; D018675:Toxicity Tests
chemical_list: D001562:Benzimidazoles; D004273:DNA, Neoplasm; C017807:bisbenzimide ethoxide trihydrochloride