title: A reduction in the butyrate producing species Roseburia spp. and Faecalibacterium prausnitzii is associated with chronic kidney disease progression.
abstract: The human gut microbiota plays an important role in human health and might also be implicated in kidney disease. The interest in butyrate producing bacteria has recently increased and is a poorly understood faecal condition in chronic kidney disease (CKD). Therefore, we evaluated differences of the butyrate producing species Roseburia spp. and Faecalibacterium prausnitzii in the faeces of Chinese patients with CKD. A case-control study was carried out for 65 CKD patients and 20 healthy controls. Differences were quantitatively validated using quantitative real-time polymerase chain reaction (qPCR). Spearman rank correlation was used to analyse the correlation between gut microbiota and clinical variables. Roseburia spp. and F. prausnitzii were significantly different in CKD patients and controls (p = 0.001; p = 0.025, respectively) and reduced more markedly in end stage renal disease (p = 0.000; p = 0.003, respectively) and microinflammation (p = 0.004; p = 0.001, respectively). Roseburia spp. and F. prausnitzii were negatively associated with C-reactive protein in plasma (r = -0.493, p = 0.00; r = -0.528, p = 0.000; respectively) and Cystatin C (r = -0.321, p = 0.006; r = -0.445, p = 0.000; respectively). They were positively associated with eGFR (r = 0.347, p = 0.002; r = 0.416, p = 0.000; respectively). The negative correlation between Roseburia spp., F. prausnitzii and CRP and renal function suggested that the depletion of butyrate producing bacteria may contribute to CKD-associated inflammation and CKD progression. Roseburia spp. and F. prausnitzii may thus serve as 'microbiomarkers'.
authors.affiliation: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, State Key Laboratory of Organ Failure Research, Ministry of Education, Guangzhou, 510000, China.
authors.forename: Shuanghong
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, State Key Laboratory of Organ Failure Research, Ministry of Education, Guangzhou, 510000, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Xie
authors.affiliation: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, State Key Laboratory of Organ Failure Research, Ministry of Education, Guangzhou, 510000, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Lv
authors.affiliation: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, State Key Laboratory of Organ Failure Research, Ministry of Education, Guangzhou, 510000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, State Key Laboratory of Organ Failure Research, Ministry of Education, Guangzhou, 510000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Deng
authors.affiliation: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, State Key Laboratory of Organ Failure Research, Ministry of Education, Guangzhou, 510000, China.
authors.forename: Lishan
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, State Key Laboratory of Organ Failure Research, Ministry of Education, Guangzhou, 510000, China. yechen_fimmu@163.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000328:Adult; D002087:Butyrates / Q000378:metabolism*; D016022:Case-Control Studies; D000068016:Eubacteriales / Q000378:metabolism*; D018450:Disease Progression; D000070037:Faecalibacterium prausnitzii / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D005260:Female; D000069196:Gastrointestinal Microbiome; D006801:Humans; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D051436:Renal Insufficiency, Chronic / Q000382:microbiology*
chemical_list: D002087:Butyrates
keywords: Butyrate; C-reactive protein; Chronic kidney disease; Gut microbiota