title: Detection and Discrimination of Non-Melanoma Skin Cancer by Multimodal Imaging.
abstract: Non-melanoma skin cancer (NMSC) belongs to the most frequent human neoplasms. Its exposed location facilitates a fast ambulant treatment. However, in the clinical practice far more lesions are removed than necessary, due to the lack of an efficient pre-operational examination procedure: Standard imaging methods often do not provide a sufficient spatial resolution. The demand for an efficient in vivo imaging technique might be met in the near future by non-linear microscopy. As a first step towards this goal, the appearance of NMSC in various microspectroscopic modalities has to be defined and approaches have to be derived to distinguish healthy skin from NMSC using non-linear optical microscopy. Therefore, in this contribution the appearance of ex vivo NMSC in a combination of coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG) and two photon excited fluorescence (TPEF) imaging-referred as multimodal imaging-is described. Analogous to H&E staining, an overview of the distinct appearances and features of basal cell and squamous cell carcinoma in the complementary modalities is derived, and is expected to boost in vivo studies of this promising technological approach.
authors.affiliation: Institute of Photonic Technology Jena (IPHT), Albert-Einstein-Straße 9, Jena 07745, Germany. sandro.heuke@ipht-jena.de.
authors.forename: Sandro
authors.initials: S
authors.lastname: Heuke
authors.affiliation: Institute of Photonic Technology Jena (IPHT), Albert-Einstein-Straße 9, Jena 07745, Germany. nadine.vogler@uni-jena.de.
authors.forename: Nadine
authors.initials: N
authors.lastname: Vogler
authors.affiliation: Institute of Photonic Technology Jena (IPHT), Albert-Einstein-Straße 9, Jena 07745, Germany. tobias.meyer@ipht-jena.de.
authors.forename: Tobias
authors.initials: T
authors.lastname: Meyer
authors.affiliation: Institute of Photonic Technology Jena (IPHT), Albert-Einstein-Straße 9, Jena 07745, Germany. denis.akimov@ipht-jena.de.
authors.forename: Denis
authors.initials: D
authors.lastname: Akimov
authors.affiliation: Department of Dermatology, Venerology and Allergology, Charit&#233;-Universit&#228;tsmedizin Berlin, Charit&#233;platz 1, Berlin 10117, Germany. Franziska.Kluschke@gmail.com.
authors.forename: Franziska
authors.initials: F
authors.lastname: Kluschke
authors.affiliation: Department of Dermatology, Venerology and Allergology, Charit&#233;-Universit&#228;tsmedizin Berlin, Charit&#233;platz 1, Berlin 10117, Germany.
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Röwert-Huber
authors.affiliation: Department of Dermatology, Venerology and Allergology, Charit&#233;-Universit&#228;tsmedizin Berlin, Charit&#233;platz 1, Berlin 10117, Germany. juergen.lademann@charite.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Lademann
authors.affiliation: Institute of Photonic Technology Jena (IPHT), Albert-Einstein-Straße 9, Jena 07745, Germany. benjamin.dietzek@uni-jena.de.|Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 4, Jena 07743, Germany. benjamin.dietzek@uni-jena.de.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Dietzek
authors.affiliation: Institute of Photonic Technology Jena (IPHT), Albert-Einstein-Straße 9, Jena 07745, Germany. rgen.Popp@ipht-jena.de.|Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 4, Jena 07743, Germany. rgen.Popp@ipht-jena.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
keywords: CARS; SHG; TPEF; basal cell carcinoma; coherent Raman scattering; multimodal imaging; non-melanoma skin cancer; skin cancer; squamous cell carcinoma