title: Sesquiterpenes from Curcuma wenyujin with their inhibitory activities on nitric oxide production in RAW 264.7 cells.
abstract: One new sesquiterpene, 7α,11-epoxy-6α-hydroxy-carabrane-4,8-dione, along with 10 known ones were isolated from the essential oil of Curcuma wenyujin Y.H. Chen et C. Ling. Their structures were established based on extensive spectroscopic analysis. The absolute configuration of compound 1 was determined by the CD analysis of the insitu formed [Rh2(OCOCF3)4] complex, and the CD data analysis based on the octane rule of cyclohexanone. The inhibitory effects of these sesquiterpenes on nitric oxide production in lipopolysaccharide-activated macrophages were also evaluated. Furthermore, the biosynthesis pathway of the isolated compounds was proposed.
authors.affiliation: a Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Department of Natural Products Chemistry , School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang , People's Republic of China.
authors.forename: Suyu
authors.initials: S
authors.lastname: Gao
authors.affiliation: a Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Department of Natural Products Chemistry , School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang , People's Republic of China.
authors.forename: Guiyang
authors.initials: G
authors.lastname: Xia
authors.affiliation: a Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Department of Natural Products Chemistry , School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang , People's Republic of China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Department of Natural Products Chemistry , School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang , People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: b Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, School of Pharmacy , Yantai University , Yantai , People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.affiliation: a Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Department of Natural Products Chemistry , School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang , People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.affiliation: a Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Department of Natural Products Chemistry , School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang , People's Republic of China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D030024:Curcuma / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D000067996:RAW 264.7 Cells; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D008070:Lipopolysaccharides; D012717:Sesquiterpenes; D009569:Nitric Oxide
keywords: Curcuma wenyujin; biosynthesis pathway; essential oil; nitric oxide; sesquiterpenoids