title: Construction and Immunological Evaluation of CpG-Au@HBc Virus-Like Nanoparticles as a Potential Vaccine.
abstract: Different types of vaccines have been developed to elicit active immunization to treat various diseases, while suffer from limitation of efficacy. Herein, a novel immunostimulatory nanocomposite (CpG-Au@HBc VLP) was rationally designed by self-assembling engineered virus-like particles encapsulating CpG-gold nanoparticle conjugates through electrostatic interactions. The monodispersed and uniformly sized CpG-Au@HBc VLP showed increased CD4(+), CD8(+) T cell numbers and stronger secretion of cytokine interferon-gamma than HBc VLPs adjuvanted with conventional Freund's adjuvant. Furthermore, the use of Au nanoparticles also generated enhanced immunogenicity of CpG and VLPs on both humoral and cellular immune pathways, as followed from increased expressions of total HBc-specific antibody titer, CD4(+) T cells, CD8(+) T cells, cytokine interleukin-4, and interferon-gamma. These findings demonstrated that CpG-Au@HBc VLP nanocomposite could induce robust cellular and humoral immune response, which could be a potential vaccine for future prophylactic and therapeutic application.
authors.affiliation: Department of Biomaterials and Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, College of Materials, Xiamen University, Xiamen, 361005, People's Republic of China.
authors.forename: Yarun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Organ Transplantation Institute, Anti-Cancer Research Center, Medical College, Xiamen University, Xiamen, 361005, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biomaterials and Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, College of Materials, Xiamen University, Xiamen, 361005, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Kang
authors.affiliation: Department of Biomaterials and Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, College of Materials, Xiamen University, Xiamen, 361005, People's Republic of China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Biomaterials and Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, College of Materials, Xiamen University, Xiamen, 361005, People's Republic of China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Shan
authors.affiliation: National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing, 102206, People's Republic of China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Bi
authors.affiliation: Department of Biomaterials and Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, College of Materials, Xiamen University, Xiamen, 361005, People's Republic of China. renlei@xmu.edu.cn.|State Key Laboratory for Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen, 361005, People's Republic of China. renlei@xmu.edu.cn.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen University, Xiamen, 361005, People's Republic of China. renlei@xmu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Ren
authors.affiliation: Organ Transplantation Institute, Anti-Cancer Research Center, Medical College, Xiamen University, Xiamen, 361005, People's Republic of China. zhgh@xmu.edu.cn.
authors.forename: Guohong
authors.initials: G
authors.lastname: Zhuang
keywords: CpG ODNs; Gold nanoparticles; Immune response; Virus-like nanoparticles