title: Profenofos induced modulation in physiological indices, genomic template stability and protein banding patterns of Anabaena sp. PCC 7120.
abstract: To understand the mechanism underlying organophosphate pesticide toxicity, cyanobacterium Anabaena PCC 7120 was subjected to varied concentrations (0, 5, 10, 20 and 30 mg L(-1)) of profenofos and the effects were investigated in terms of changes in cellular physiology, genomic template stability and protein expression pattern. The supplementation of profenofos reduced the growth, total pigment content and photosynthetic efficiency of the test organism in a dose dependent manner with maximum toxic effect at 30 mg L(-1). The high fluorescence intensity of 2', 7' -dichlorofluorescin diacetate and increased production of malondialdehyde confirmed the prevalence of acute oxidative stress condition inside the cells of the cyanobacterium. Rapid amplified polymorphic DNA (RAPD) fingerprinting and SDS-PAGE analyses showed a significant alteration in the banding patterns of DNA and proteins respectively. A marked increase in superoxide dismutase, catalase, peroxidase activity and a concomitant reduction in glutathione content indicated their possible role in supporting the growth of Anabaena 7120 up to 20 mg L(-1). These findings suggest that the uncontrolled use of profenofos in the agricultural fields may not only lead to the destruction of the cyanobacterial population, but it would also disturb the nutrient dynamics and energy flow.
authors.affiliation: a Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University , Varanasi , India.
authors.forename: Balkrishna
authors.initials: B
authors.lastname: Tiwari
authors.affiliation: a Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University , Varanasi , India.
authors.forename: Sindhunath
authors.initials: S
authors.lastname: Chakraborty
authors.affiliation: a Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University , Varanasi , India.
authors.forename: Savita
authors.initials: S
authors.lastname: Singh
authors.affiliation: a Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University , Varanasi , India.
authors.forename: Arun K
authors.initials: AK
authors.lastname: Mishra
mesh_terms: D017033:Anabaena / Q000187:drug effects / Q000201:enzymology*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism*; D048028:DNA, Algal / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D007306:Insecticides / Q000378:metabolism / Q000633:toxicity*; D008315:Malondialdehyde / Q000378:metabolism / Q000633:toxicity*; D063086:Organothiophosphates / Q000378:metabolism / Q000633:toxicity*; D010788:Photosynthesis / Q000187:drug effects; D010829:Physiological Phenomena / Q000187:drug effects*; D019105:Random Amplified Polymorphic DNA Technique; D013482:Superoxide Dismutase / Q000187:drug effects
chemical_list: D048028:DNA, Algal; D007306:Insecticides; D063086:Organothiophosphates; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Anabaena sp. PCC7120; Profenofos; genomic template stability; proteins pattern