title: [Determination of 6 kinds of plant growth regulator in bean sprout by ultra high performance liquid chromatography-tandem mass spectrometry].
abstract: OBJECTIVE
  A method for the simultaneous determination of 6 plant growth regulator (PGR) residues in bean sprout was developed by ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). 6-Benzylaminopurine, isopentennyladenine, 4-chlorophenoxyacetic acid, 4-fluorophenoxyacetic acid, indole-3- acetic acid and indole-3-butyric acid were concerned.
  
  
  METHODS
  Bean sprout samples were extracted by acetonitrile and QuEChERS extraction kit, purified by C18 powers. After centrifugation, the sample liquids was diluted 10 times by ultrapure water. The chromatographic analysis was carried out on an waters acquity UPLC BEH C18 column( 100 mm x 2.1 mm, 1.7 microm). The analyzer confirmed and quantified by mass spectrum of triple quadrupole in the multiple reaction monitoring (MRM) mode and quantified by matrix-matched external standard method.
  
  
  RESULTS
  The calibration curves showed good linearity in each range with correlation coefficients greater than 0.998. 3 levels spiked recoveries were carried out using blank bean sprout extraction as substrate, the recoveries ranged from 84.2% to 107.5%, the relative standard deviations (RSDs) ranged from 3.08% to 12.71%. The qualitative limits of detections (S/N = 3) were 0.03-3.0 microg/kg and the quantitative limits(S/N = 10) were 0.1-10.0 microg/kg for the 6 PGRs.
  
  
  CONCLUSIONS
  The method is simple and easy to operate, with less organic reagent, high sensitivity and good stability. It is suitable for the detection of 6 kinds of plant growth regulators in bean sprouts.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Sai
authors.initials: S
authors.lastname: Fan
authors.forename: Guohua
authors.initials: G
authors.lastname: Wu
authors.forename: Rong
authors.initials: R
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhao
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000031:analogs & derivatives / Q000032:analysis; D001593:Benzyl Compounds; D002851:Chromatography, High Pressure Liquid; D007210:Indoleacetic Acids / Q000032:analysis; D007211:Indoles / Q000032:analysis; D007541:Isopentenyladenosine / Q000032:analysis; D007701:Kinetin / Q000032:analysis; D027805:Phaseolus / Q000737:chemistry*; D010937:Plant Growth Regulators / Q000032:analysis*; D011687:Purines; D053719:Tandem Mass Spectrometry
chemical_list: D001593:Benzyl Compounds; D007210:Indoleacetic Acids; D007211:Indoles; D010937:Plant Growth Regulators; D011687:Purines; C014612:indolebutyric acid; C001478:N(6)-(delta(2)-isopentenyl)adenine; D015084:2,4-Dichlorophenoxyacetic Acid; C007017:4-chlorophenoxyacetic acid; C030737:indoleacetic acid; D007541:Isopentenyladenosine; C480551:benzylaminopurine; D007701:Kinetin