title: The Effect of Skin Debris on Gunshot Residue Sampling and Detection.
abstract: These experiments were designed to determine whether skin debris (desquamated epithelial cells and apparent skin oils) affects gunshot residue (GSR) particle detection on the sticky tape lift samples prepared for scanning electron microscopy (SEM). A dabbing experiment showed that GSR particles accumulate not only on the adhesive surface of the sampler, but also on the epithelial cell surfaces. Samplers were loaded with target GSR followed by dabbing 30 times on the back of a hand. Backscatter electron images were taken at 20 kV and for some at 30 kV of the same areas. The samplers were then treated with a sodium/calcium hypochlorite solution (bleach) to remove skin debris and again imaged in the SEM. Comparison of these images shows more GSR particles will likely be revealed at 30 kV than 20 kV and more particles revealed by the bleach treatment in an automated SEM system.
authors.affiliation: Meixa Tech, P.O. Box 844, Cardiff, CA, 92007.
authors.forename: Bryan R
authors.initials: BR
authors.lastname: Burnett
mesh_terms: D006225:Hand; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012867:Skin / Q000473:pathology*; D013048:Specimen Handling; D014948:Wounds, Gunshot / Q000473:pathology*
keywords: backscatter electron imaging; bleach; forensic science; gunshot residue; scanning electron microscopy; skin debris