title: Automated Non-invasive Video-Microscopy of Oyster Spat Heart Rate during Acute Temperature Change: Impact of Acclimation Temperature.
abstract: We developed an automated, non-invasive method to detect real-time cardiac contraction in post-larval (1.1-1.7 mm length), juvenile oysters (i.e., oyster spat) via a fiber-optic trans-illumination system. The system is housed within a temperature-controlled chamber and video microscopy imaging of the heart was coupled with video edge-detection to measure cardiac contraction, inter-beat interval, and heart rate (HR). We used the method to address the hypothesis that cool acclimation (10°C vs. 22°C-Ta10 or Ta22, respectively; each n = 8) would preserve cardiac phenotype (assessed via HR variability, HRV analysis and maintained cardiac activity) during acute temperature changes. The temperature ramp (TR) protocol comprised 2°C steps (10 min/experimental temperature, Texp) from 22°C to 10°C to 22°C. HR was related to Texp in both acclimation groups. Spat became asystolic at low temperatures, particularly Ta22 spat (Ta22: 8/8 vs. Ta10: 3/8 asystolic at Texp = 10°C). The rate of HR decrease during cooling was less in Ta10 vs. Ta22 spat when asystole was included in analysis (P = 0.026). Time-domain HRV was inversely related to temperature and elevated in Ta10 vs. Ta22 spat (P < 0.001), whereas a lack of defined peaks in spectral density precluded frequency-domain analysis. Application of the method during an acute cooling challenge revealed that cool temperature acclimation preserved active cardiac contraction in oyster spat and increased time-domain HRV responses, whereas warm acclimation enhanced asystole. These physiologic changes highlight the need for studies of mechanisms, and have translational potential for oyster aquaculture practices.
authors.affiliation: Biomedical and Molecular Sciences, Queen's University Kingston, ON, Canada.
authors.forename: Nicolle J
authors.initials: NJ
authors.lastname: Domnik
authors.affiliation: Institute for Marine and Antarctic Studies, University of Tasmania Hobart, TAS, Australia.
authors.forename: Elias T
authors.initials: ET
authors.lastname: Polymeropoulos
authors.affiliation: Commonwealth Science and Industry Research Organization, Agriculture Flagship Hobart, TAS, Australia.
authors.forename: Nicholas G
authors.initials: NG
authors.lastname: Elliott
authors.affiliation: Zoology, University of Tasmania Hobart, TAS, Australia.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Frappell
authors.affiliation: Biomedical and Molecular Sciences, Queen's UniversityKingston, ON, Canada; Medicine, Division of Respirology, Queen's UniversityKingston, ON, Canada.
authors.forename: John T
authors.initials: JT
authors.lastname: Fisher
keywords: acclimation temperature; aquaculture; heart rate; heart rate variability; oyster; oyster spat; video microscopy