title: Antibacterial effect of copper-bearing titanium alloy (Ti-Cu) against Streptococcus mutans and Porphyromonas gingivalis.
abstract: Formation of bacterial biofilms on dental implant material surfaces (titanium) may lead to the development of peri-implant diseases influencing the long term success of dental implants. In this study, a novel Cu-bearing titanium alloy (Ti-Cu) was designed and fabricated in order to efficiently kill bacteria and discourage formation of biofilms, and then inhibit bacterial infection and prevent implant failure, in comparison with pure Ti. Results from biofilm based gene expression studies, biofilm growth observation, bacterial viability measurements and morphological examination of bacteria, revealed antimicrobial/antibiofilm activities of Ti-Cu alloy against the oral specific bacterial species, Streptococcus mutans and Porphyromonas gingivalis. Proliferation and adhesion assays with mesenchymal stem cells, and measurement of the mean daily amount of Cu ion release demonstrated Ti-Cu alloy to be biocompatible. In conclusion, Ti-Cu alloy is a promising dental implant material with antimicrobial/antibiofilm activities and acceptable biocompatibility.
authors.affiliation: Northeastern University, 3-11 Wenhua Road, Shenyang 110819, China.|Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Institute of Dentistry, Barts and The London School of Medicine &Dentistry, Queen Mary University of London, Newark Street, London E1 2AT UK.
authors.forename: Kaveh
authors.initials: K
authors.lastname: Memarzadeh
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Institute of Dentistry, Barts and The London School of Medicine &Dentistry, Queen Mary University of London, Newark Street, London E1 2AT UK.
authors.forename: Robert P
authors.initials: RP
authors.lastname: Allaker
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ren
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
mesh_terms: D000497:Alloys / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology*; D005453:Fluorescence; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D021621:Imaging, Three-Dimensional; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000235:genetics / Q000648:ultrastructure; D051381:Rats; D013295:Streptococcus mutans / Q000187:drug effects* / Q000235:genetics / Q000648:ultrastructure
chemical_list: D000497:Alloys; D000900:Anti-Bacterial Agents; C586532:Ti-Cu alloy; D003300:Copper