title: Association of Mixed Lineage Kinase Domain-Like Protein Expression With Prognosis in Patients With Colon Cancer.
abstract: The mixed lineage kinase domain-like protein has recently been identified as a key downstream component of tumor necrosis factor-induced necroptosis, which is an important pathway of cancer cell death. The goal of the current study is to explore the expression of mixed lineage kinase domain-like protein in colon cancer tissues and evaluate the prognostic value in patients with colon cancer.
  
  
  
  We collected normal and cancer colon tissues from 135 patients diagnosed with colon cancer after radical operation during July 2007 to April 2009 at The Affiliated Hospital of Qingdao University. Immunohistochemistry analysis was scored using an established scoring system. Kaplan-Meier survival curves were generated for recurrence-free survival and overall survival for all patients and 2 subsets of patients. The relationship between mixed lineage kinase domain-like protein expression and prognosis parameter (recurrence-free survival, overall survival) was analyzed by univariate and multivariate Cox regression analyses.
  
  
  
  The median age of all patients was 67 years and 56.3% were male. Low expression of mixed lineage kinase domain-like protein was associated with decreased overall survival (78.6 vs 81.2 months; P = .011) in all patients. In the subset of 79 patients who received adjuvant chemotherapy, low expression of mixed lineage kinase domain-like protein was associated with decreased recurrence-free survival (60.4 vs 72.8 months; P = .032) and decreased overall survival (66.3 vs 72.9 months; P = .005). Low expression of mixed lineage kinase domain-like protein was associated with decreased overall survival (74.9 vs 79.8 months; P = .006) and recurrence-free survival (69.6 vs 78.8 months; P = .005) among patients with Tumor Node Metastasis (TNM) stage II colon cancer.
  
  
  
  Low expression of mixed lineage kinase domain-like protein was associated with decreased overall survival in all patient-group with resected colon cancer. It is associated with decreased recurrence-free survival and overall survival in the subset of patients who receive adjuvant chemotherapy and patients who were TNM stage II. Mixed lineage kinase domain-like protein may provide important prognostic information in patients with colon cancer.
authors.affiliation: 1 Department of Surgery, Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, Shandong, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.affiliation: 2 Department of Oncology, Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, Shandong, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.affiliation: 2 Department of Oncology, Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, Shandong, China.
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Ding
authors.affiliation: 2 Department of Oncology, Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, Shandong, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Qi
authors.affiliation: 2 Department of Oncology, Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, Shandong, China.
authors.forename: Pei-Hua
authors.initials: PH
authors.lastname: Zhang
authors.affiliation: 2 Department of Oncology, Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, Shandong, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lv
authors.affiliation: 2 Department of Oncology, Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, Shandong, China.
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Qiu
authors.affiliation: 1 Department of Surgery, Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, Shandong, China.
authors.forename: Zhen-Qing
authors.initials: ZQ
authors.lastname: Sun
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D017024:Chemotherapy, Adjuvant; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000628:therapy; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D016016:Proportional Hazards Models; D011494:Protein Kinases / Q000378:metabolism*; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; C570693:MLKL protein, human; D011494:Protein Kinases
keywords: MLKL; colon cancer; necroptosis; prognostic value