title: DNA Cleavage and Condensation Activities of Mono- and Binuclear Hybrid Complexes and Regulation by Graphene Oxide.
abstract: Hybrid complexes with N,N'-bis(2-benzimidazolylmethyl)amine and cyclen moieties are novel enzyme mimics and controlled DNA release materials, which could interact with DNA through three models under different conditions. In this paper, the interactions between plasmid DNA and seven different complexes were investigated, and the methods to change the interaction patterns by graphene oxide (GO) or concentrations were also investigated. The cleavage of pUC19 DNA promoted by target complexes were via hydrolytic or oxidative mechanisms at low concentrations ranging from 3.13 × 10(-7) to 6.25 × 10(-5) mol/L. Dinuclear complexes 2a and 2b can promote the cleavage of plasmid pUC19 DNA to a linear form at pH values below 7.0. Furthermore, binuclear hybrid complexes could condense DNA as nanoparticles above 3.13 × 10(-5) mol/L and partly release DNA by graphene oxide with π-π stacking. Meanwhile, the results also reflected that graphene oxide could prevent DNA from breaking down. Cell viability assays showed dinuclear complexes were safe to normal human hepatic cells at relative high concentrations. The present work might help to develop novel strategies for the design and synthesis of DNA controllable releasing agents, which may be applied to gene delivery and also to exploit the new application for GO.
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China. lishuo@cqut.edu.cn.|School of Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China. lishuo@cqut.edu.cn.
authors.forename: Shuo
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China. dai_mingxing@yeah.net.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Dai
authors.affiliation: Jiangsu Key Laboratory of Target Drug and Clinical Application, Xuzhou Medical College, Xuzhou 221004, China. gaoshan210@163.com.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: School of Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China. jiangby@cqut.edu.cn.
authors.forename: Bingying
authors.initials: B
authors.lastname: Jiang
authors.affiliation: School of Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China. xujunqiang@cqut.edu.cn.
authors.forename: Junqiang
authors.initials: J
authors.lastname: Xu
authors.affiliation: School of Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China. dewen@cqut.edu.cn.
authors.forename: Dewen
authors.initials: D
authors.lastname: Zhou
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China. zwgu@scu.edu.cn.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Gu
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry*; D053837:DNA Cleavage / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009690:Nucleic Acid Conformation; D010087:Oxides / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry; D013997:Time Factors
chemical_list: D016166:Free Radical Scavengers; D010087:Oxides; D006108:Graphite; D004247:DNA
keywords: DNA condenstion; DNA release; N,N′-bis(2-benzimidazolylmethyl)amine; cyclen; enzyme mimic; graphene oxide