title: Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies.
abstract: Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can induce various clinical tumors. The capsid protein P27 is the group-specific antigen of ALV and has many viral antigen sites that are easy to detect. In this study, we produced a monoclonal antibody (mAb), 3A9, that is specific for the P27 protein. A series of partially overlapping peptides were screened to define (181)PPSAR(185) as the minimal linear epitope recognized by mAb 3A9. The identified epitope could be recognized by chicken anti-ALV and mouse anti-ALV P27 sera. The epitope was highly conserved among a number of ALV-A, ALV-B and ALV-J strains. MAb 3A9 might be a valuable tool for the development of new immunodiagnostic approaches for ALV, and the defined linear epitope might help further our understanding of the antigenic structure of the P27 protein.
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nan Gang District, Harbin, Heilongjiang Province, People's Republic of China.|New Hope Liuhe Co., Ltd., Qingdao, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Li
authors.affiliation: New Hope Liuhe Co., Ltd., Qingdao, China.
authors.forename: Liting
authors.initials: L
authors.lastname: Qin
authors.affiliation: Harbin National Engineering Research Center of Veterinary Biologics Co., Ltd., Harbin, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhu
authors.affiliation: New Hope Liuhe Co., Ltd., Qingdao, China.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Sun
authors.affiliation: New Hope Liuhe Co., Ltd., Qingdao, China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Cui
authors.affiliation: New Hope Liuhe Co., Ltd., Qingdao, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nan Gang District, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Xiaole
authors.initials: X
authors.lastname: Qi
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nan Gang District, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nan Gang District, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Honglei
authors.initials: H
authors.lastname: Gao
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nan Gang District, Harbin, Heilongjiang Province, People's Republic of China. ylg@hvri.ac.cn.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nan Gang District, Harbin, Heilongjiang Province, People's Republic of China. xmw@hvri.ac.cn.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology / Q000302:isolation & purification; D001354:Avian Leukosis Virus / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D002645:Chickens; D017124:Conserved Sequence; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology*; D005260:Female; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; D018985:Epitopes, B-Lymphocyte
keywords: P27 protein; avian leukosis virus; epitope; monoclonal antibodies