title: Molecular and phylogenetic analysis ofAnaplasmaspp. in sheep and goats from six provinces of China.
abstract: Members of the genusAnaplasmaare important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence ofAnaplasmaspp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction (PCR) and DNA sequencing were conducted to determine the prevalence ofAnaplasma (A.) phagocytophilum, A. ovisandA. bovistargeting the 16S ribosomal RNA or the major surface protein 4 gene. PCR revealedAnaplasmain 39.0% (240/621) of sheep and 45.5% (323/710) of goats. The most frequently detected species wasA. ovis(88/621, 14.2% for sheep; 129/710, 18.2% for goats), followed byA. bovis(60/621, 9.7% for sheep; 74/710, 10.4% for goats) andA. phagocytophilum(33/621, 5.3% for sheep; 15/710, 2.1% for goats). Additionally, eight sheep and 20 goats were found to be infected with three pathogens simultaneously. DNA sequencing confirmed the presence of these threeAnaplasmaspecies in the investigated areas, and phylogenetic analysis indicated that there was geographic segregation to a certain extent, as well as a relationship between the host and cluster ofA. ovis.The results of the present study provide valuable data that helps understand the epidemiology of anaplasmosis in ruminants from China.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Lv
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Cui
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Rongjun
authors.initials: R
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Fuchun
authors.initials: F
authors.lastname: Jian
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Longxian
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Changshen
authors.initials: C
authors.lastname: Ning
mesh_terms: D000709:Anaplasma / Q000235:genetics / Q000502:physiology*; D000712:Anaplasmosis / Q000453:epidemiology* / Q000469:parasitology; D000818:Animals; D018834:Chaperonin 60 / Q000235:genetics; D002681:China / Q000453:epidemiology; D016054:DNA, Protozoan / Q000235:genetics; D005260:Female; D015511:Goat Diseases / Q000453:epidemiology*; D006041:Goats; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015800:Protozoan Proteins / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000469:parasitology; D013045:Species Specificity
chemical_list: D018834:Chaperonin 60; D016054:DNA, Protozoan; D015800:Protozoan Proteins; D012336:RNA, Ribosomal, 16S
keywords: Anaplasma bovis; Anaplasma ovis; Anaplasma phagocytophilum; phylogenetic analysis; prevalence