title: Effect of Modified Alkaline Supplementation on Syngenic Melanoma Growth in CB57/BL Mice.
abstract: Tumor extracellular acidity is a hallmark of malignant cancers. Thus, in this study we evaluated the effects of the oral administration of a commercially available water alkalizer (Basenpulver®) (BP) on tumor growth in a syngenic melanoma mouse model. The alkalizer was administered daily by oral gavage starting one week after tumor implantation in CB57/BL mice. Tumors were calipered and their acidity measured by in vivo MRI guided 31P MRS. Furthermore, urine pH was monitored for potential metabolic alkalosis. BP administration significantly reduced melanoma growth in mice; the optimal dose in terms of tolerability and efficacy was 8 g/l (p< 0.05). The in vivo results were supported by in vitro experiments, wherein BP-treated human and murine melanoma cell cultures exhibited a dose-dependent inhibition of tumor cell growth. This investigation provides the first proof of concept that systemic buffering can improve tumor control by itself and that this approach may represent a new strategy in prevention and/or treatment of cancers.
authors.affiliation: Department of Drug Research and Medicine Evaluation, National Institute of Health, Rome, Italy.
authors.forename: Tommaso
authors.initials: T
authors.lastname: Azzarito
authors.affiliation: Department of Drug Research and Medicine Evaluation, National Institute of Health, Rome, Italy.
authors.forename: Luana
authors.initials: L
authors.lastname: Lugini
authors.affiliation: SAFU Department, Regina Elena Cancer Institute, Rome, Italy.
authors.forename: Enrico Pierluigi
authors.initials: EP
authors.lastname: Spugnini
authors.affiliation: Department of Cell Biology and Neurosciences, National Institute of Health, Rome, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Canese
authors.affiliation: Department of Drug Research and Medicine Evaluation, National Institute of Health, Rome, Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Gugliotta
authors.affiliation: Department of Drug Research and Medicine Evaluation, National Institute of Health, Rome, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Fidanza
authors.affiliation: Department of Drug Research and Medicine Evaluation, National Institute of Health, Rome, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Fais
mesh_terms: D000821:Animal Feed; D000818:Animals; D045744:Cell Line, Tumor; D019587:Dietary Supplements; D004195:Disease Models, Animal; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008279:Magnetic Resonance Imaging / Q000379:methods; D008545:Melanoma / Q000473:pathology*; D008546:Melanoma, Experimental; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D047368:Tumor Burden