title: Performance and microbial community of simultaneous anammox and denitrification (SAD) process in a sequencing batch reactor.
abstract: A sequencing batch reactor (SBR) was used to test the simultaneous anammox and denitrification process. Optimal nitrogen removal was achieved with chemical oxygen demand (COD) of 150mg/L, during which almost all of ammonia, nitrite and nitrate could be removed. Organic matter was a key factor to regulate the synergy of anammox and denitrification. Both experimental ΔNO2(-)-N/ΔNH4(+)-N and ΔNO3(-)-N/ΔNH4(+)-N values deviated from their theoretical values with increasing COD. Denitrifying bacteria exhibited good diversity and abundance, but the diversity of anammox bacteria was less abundant. Brocadia sinica was able to grow in the presence of organic matter and tolerate high nitrite concentration. Anammox bacteria were predominant at low COD contents, while denitrifying bacteria dominated the microbial community at high COD contents. Anammox and denitrifying bacteria could coexist in one reactor to achieve the simultaneous carbon and nitrogen removal through the synergy of anammox and denitrification.
authors.affiliation: School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China. Electronic address: ljin0532@126.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-environmental Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Qiang
authors.affiliation: School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China.
authors.forename: Deshuang
authors.initials: D
authors.lastname: Yu
authors.affiliation: National Marine Environmental Forecasting Center, State Oceanic Administration, Beijing 100081, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China.
authors.forename: Peiyu
authors.initials: P
authors.lastname: Zhang
authors.affiliation: School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000295:instrumentation*; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D058440:Denitrification; D009584:Nitrogen / Q000302:isolation & purification; D010084:Oxidation-Reduction; D010802:Phylogeny
chemical_list: D000641:Ammonia; D009584:Nitrogen
keywords: Microbial community; Nitrogen removal; Organic matter concentration; Simultaneous anammox and denitrification (SAD) process