title: Capillary electrophoresis coupled to contactless conductivity detection for analysis of amino acids of agricultural interest in composting.
abstract: Composting is a sustainable approach to manage animal and vegetal waste generated in the Fundação Parque Zoológico de São Paulo. The resulting compost is often used in ZOO's premises as an organic fertilizer for the production of vegetables, which is further used to feed the animals. The composting product provides many forms of mineral and also amino acids (AA) that are absorbed by plants as nutrients. Since most amino acids absorb only slightly or not at all in the UV wavelengths, we developed a method for the determination of AA of agricultural interest in the composting samples. Due to the complexity of samples, we used ion exchange chromatography for the purification of AA prior to analysis. The proposed CZE-C4D method allowed a separation of the AA in a short analysis time (less than 3.0 min), with great linearity (with R2ranging from 0.993 to 0.998). Using a BGE of 10 mmol/L TEA, reduction of high-frequency noise and lower baseline fluctuations were obtained. The LOQ for the five AA were around 35 μmol/L, and were adequate for our purpose. In addition, the method showed good precision (RSD of peak area and migration time less than 1.55 and 1.16%, respectively).
authors.affiliation: Universidade Federal de São Paulo - UNIFESP - Rua 3 de Maio, São Paulo, Brazil.|Instituto de Química de São Carlos - IQSC - USP - Avenida Trabalhador São-carlense 400, São Carlos, Brazil.
authors.forename: Luciana Teresa Dias
authors.initials: LT
authors.lastname: Cappelini
authors.affiliation: Instituto de Química de São Carlos - IQSC - USP - Avenida Trabalhador São-carlense 400, São Carlos, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: de Fátima Menegoci Eugênio
authors.affiliation: Instituto de Química de São Carlos - IQSC - USP - Avenida Trabalhador São-carlense 400, São Carlos, Brazil.
authors.forename: Paulo Augusto Gomes Carneiro
authors.initials: PA
authors.lastname: Leão
authors.affiliation: Instituto de Química de São Carlos - IQSC - USP - Avenida Trabalhador São-carlense 400, São Carlos, Brazil.
authors.forename: Juliana Vieira
authors.initials: JV
authors.lastname: Alberice
authors.affiliation: Instituto de Química de São Carlos - IQSC - USP - Avenida Trabalhador São-carlense 400, São Carlos, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Urbaczek
authors.affiliation: Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de Sao Paulo - UNIFESP, Rua Prof. Artur Riedel, 275, Diadema, Brazil.
authors.forename: Nilson Antonio
authors.initials: NA
authors.lastname: Assunção
authors.affiliation: Universidade Federal de São Paulo - UNIFESP - Rua 3 de Maio, São Paulo, Brazil.
authors.forename: Luiz
authors.initials: L
authors.lastname: Juliano
authors.affiliation: Instituto de Química de São Carlos - IQSC - USP - Avenida Trabalhador São-carlense 400, São Carlos, Brazil. emanuel@iqsc.usp.br.|Instituto Nacional de Ciência e Tecnologia de Bioanalítica - INCTBio, Campinas, Brazil. emanuel@iqsc.usp.br.
authors.forename: Emanuel
authors.initials: E
authors.lastname: Carrilho
mesh_terms: D000383:Agriculture; D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012987:Soil / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D012987:Soil
keywords: Amino acids; CE-C4D; Cation exchange chromatography; Composting