title: Interaction properties of human TRAMP-like proteins and their role in pre-rRNA 5'ETS turnover.
abstract: In yeast, the Trf4/5-Air1/2-Mtr4 polyadenylation (TRAMP) complex acts as a cofactor for the nuclear exosome to promote degradation of various RNAs. However, the corresponding machinery in mammals is less characterized. We analyzed the interactions of the human TRAMP-like proteins, PAPD5, ZCCHC7, and MTR4, with the nuclear exosome. PAPD5 and ZCCHC7 exhibited mutual interactions in presence of the exosome catalytic subunit RRP6, whereas MTR4 was dispensable for their assembly. Furthermore, the human TRAMP-like proteins were involved in the RRP6-catalyzed turnover of pre-rRNA 5'ETS fragments. These results suggest the significant role for RRP6 in the assembly of TRAMP-like proteins during nucleolar RNA surveillance.
authors.affiliation: Laboratory of Molecular and Cellular Biochemistry, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Sudo
authors.affiliation: Laboratory of Molecular and Cellular Biochemistry, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Nozaki
authors.affiliation: Laboratory of Molecular and Cellular Biochemistry, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Uno
authors.affiliation: Laboratory of Molecular and Cellular Biochemistry, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
authors.forename: Yo-Ichi
authors.initials: Y
authors.lastname: Ishida
authors.affiliation: Laboratory of Molecular and Cellular Biochemistry, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Nagahama
mesh_terms: D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism; D063326:Exosome Multienzyme Ribonuclease Complex / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism*; D012316:RNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D012322:RNA Precursors / Q000235:genetics; D012323:RNA Processing, Post-Transcriptional; D020871:RNA Stability / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D012322:RNA Precursors; D014157:Transcription Factors; C545342:ZCCHC7 protein, human; D012316:RNA Nucleotidyltransferases; C567954:TENT4B protein, human; D005095:Exoribonucleases; D063326:Exosome Multienzyme Ribonuclease Complex; C077044:EXOSC10 protein, human; C512513:MTREX protein, human; D020365:RNA Helicases
keywords: PAP-associated domain-containing protein 5; RRP6; Trf4/5-Air1/2-Mtr4 polyadenylation; exosome; pre-rRNA; zinc finger CCHC domain-containing protein 7