title: IL-10 Promotes Neurite Outgrowth and Synapse Formation in Cultured Cortical Neurons after the Oxygen-Glucose Deprivation via JAK1/STAT3 Pathway.
abstract: As a classic immunoregulatory and anti-inflammatory cytokine, interleukin-10 (IL-10) provides neuroprotection in cerebral ischemia in vivo or oxygen-glucose deprivation (OGD)-induced injury in vitro. However, it remains blurred whether IL-10 promotes neurite outgrowth and synapse formation in cultured primary cortical neurons after OGD injury. In order to evaluate its effect on neuronal apoptosis, neurite outgrowth and synapse formation, we administered IL-10 or IL-10 neutralizing antibody (IL-10NA) to cultured rat primary cortical neurons after OGD injury. We found that IL-10 treatment activated the Janus kinase 1 (JAK1)/signal transducers and activators of transcription 3 (STAT3) signaling pathway. Moreover, IL-10 attenuated OGD-induced neuronal apoptosis by down-regulating the Bax expression and up-regulating the Bcl-2 expression, facilitated neurite outgrowth by increasing the expression of Netrin-1, and promoted synapse formation in cultured primary cortical neurons after OGD injury. These effects were partly abolished by JAK1 inhibitor GLPG0634. Contrarily, IL-10NA produced opposite effects on the cultured cortical neurons after OGD injury. Taken together, our findings suggest that IL-10 not only attenuates neuronal apoptosis, but also promotes neurite outgrowth and synapse formation via the JAK1/STAT3 signaling pathway in cultured primary cortical neurons after OGD injury.
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.|Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.|Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.affiliation: Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.|Department of Rehabilitation, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.
authors.forename: Yixian
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.|Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Longzai
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.|Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Jianhao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.|Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.|Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Mouwei
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.|Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Zezhong
authors.initials: Z
authors.lastname: Zhuang
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.|Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Houwei
authors.initials: H
authors.lastname: Du
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.|Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.|Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.|Department of Rehabilitation, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D048430:Cell Shape; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D005260:Female; D005947:Glucose / Q000172:deficiency*; D016753:Interleukin-10 / Q000494:pharmacology*; D053613:Janus Kinase 1 / Q000378:metabolism; D000075388:Netrin-1 / Q000378:metabolism; D000071446:Neuronal Outgrowth / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D053701:Receptors, Interleukin-10 / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013569:Synapses / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D053701:Receptors, Interleukin-10; D050796:STAT3 Transcription Factor; D016753:Interleukin-10; D000075388:Netrin-1; D053613:Janus Kinase 1; D005947:Glucose; D010100:Oxygen