title: [Comparative immunohistochemical assessment of the effect of repetitive anesthesia with isoflurane and sevoflurane on rat liver].
abstract: OBJECTIVE
  Inhalation anesthetics are used in human, as well as veterinary medical practice. In the present study we investigated the effect of isoflurane and sevoflurane on rat hepatocytes.
  
  
  METHODS
  A total of 40 Wistar female rats were used in this study. Animals were divided in groups of 5 rats. Groups IM, SM served as control groups. Groups I1, I2, I3 were used to study isoflurane and S1, S2, S3 for sevoflurane study. They were anesthetized 3 times, for 2h long, at 2 days interval with a concentration of: 1.5% isoflurane (I1, I2, I3) and 2% sevoflurane (S1, S2, S3). The oxygen supply throughout the anesthesia was 1LO2/min. Groups IM, IS, I1, S1 were sacrificed immediately after the last anesthesia. Groups I2, S2 were sacrificed 6h after the last anesthesia, and groups I3, S3, 24h post-anesthesia. Liver samples were harvested to highlight caspase-3 in apoptotic hepatocytes.
  
  
  RESULTS
  Following isoflurane administration, there were less than 1% cells in apoptosis highlighted in rat livers from groups IM, I1 and I2. At 24h post-anesthesia (group I3), a small number of apoptotic hepatocytes was highlighted (around 3.23% cells in apoptosis), with a strictly periacinar disposition, randomly distributed in a small number of hepatic lobules. After sevoflurane administration, less than 1% apoptotic hepatocytes were identified at all control moments throughout the study.
  
  
  CONCLUSIONS
  The results suggest that the anesthetics do not present a considerable hepatotoxicity. The comparative assessment of the two anesthetics shows that sevoflurane is superior to isoflurane.
authors.affiliation: Department of Cell Biology, Histology and Embriology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romênia.
authors.forename: Flavia
authors.initials: F
authors.lastname: Ruxanda
authors.affiliation: Department of Pathological Anatomy, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romênia. Electronic address: adrianfloringal@yahoo.com.
authors.forename: Adrian Florin
authors.initials: AF
authors.lastname: Gal
authors.affiliation: Department of Implantology, Faculty of Medicine and Pharmacy, University of Oradea, Oradea, Romênia.
authors.forename: Cristian
authors.initials: C
authors.lastname: Raţiu
authors.affiliation: Department of Cell Biology, Histology and Embriology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romênia.
authors.forename: Viorel
authors.initials: V
authors.lastname: Miclăuş
authors.affiliation: Department of Cell Biology, Histology and Embriology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romênia.
authors.forename: Vasile
authors.initials: V
authors.lastname: Rus
authors.affiliation: Department of Anesthesiology and Surgical Propedeutics, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romênia.
authors.forename: Liviu Ioan
authors.initials: LI
authors.lastname: Oana
keywords: Anestesia; Anesthesia; Caspase‐3; Fígado; Isoflurane; Isoflurano; Liver; Sevoflurane; Sevoflurano