title: Mature red blood cells: from optical model to inverse light-scattering problem.
abstract: We propose a method for characterization of mature red blood cells (RBCs) morphology, based on measurement of light-scattering patterns (LSPs) of individual RBCs with the scanning flow cytometer and on solution of the inverse light-scattering (ILS) problem for each LSP. We considered a RBC shape model, corresponding to the minimal bending energy of the membrane with isotropic elasticity, and constructed an analytical approximation, which allows rapid simulation of the shape, given the diameter and minimal and maximal thicknesses. The ILS problem was solved by the nearest-neighbor interpolation using a preliminary calculated database of 250,000 theoretical LSPs. For each RBC in blood sample we determined three abovementioned shape characteristics and refractive index, which also allows us to calculate volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration and content.
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia.
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Gilev
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia.
authors.forename: Maxim A
authors.initials: MA
authors.lastname: Yurkin
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia.
authors.forename: Ekaterina S
authors.initials: ES
authors.lastname: Chernyshova
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State Medical University, Krasny Prospect 52, 630091, Novosibirsk, Russia.
authors.forename: Dmitry I
authors.initials: DI
authors.lastname: Strokotov
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Chernyshev
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia; Novosibirsk State Medical University, Krasny Prospect 52, 630091, Novosibirsk, Russia.
authors.forename: Valeri P
authors.initials: VP
authors.lastname: Maltsev
keywords: (170.1530) Cell analysis; (170.3890) Medical optics instrumentation; (290.5820) Scattering measurements