title: Variations in Protein Concentration and Nitrogen Sources in Different Positions of Grain in Wheat.
abstract: The distribution patterns of total protein and protein components in different layers of wheat grain were investigated using the pearling technique, and the sources of different protein components and pearling fractions were identified using (15)N isotope tracing methods. It was found that N absorbed from jointing to anthesis (JA) and remobilized to the grain after anthesis was the principal source of grain N, especially in the outer layer. For albumin and globulin, the amount of N absorbed during different stages all showed a decreasing trend from the surface layer to the center part. Whereas, for globulin and glutenin, the N absorbed after anthesis accounted for the main part indicating that for storage protein, the utilization of N assimilated after anthesis is greater than that of the stored N assimilated before anthesis. It is concluded that manipulation of the N application rate during different growth stages could be an effective approach to modulate the distribution of protein fractions in pearled grains for specific end-uses.
authors.affiliation: National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing Agricultural UniversityNanjing, China; Department of Plant and Environmental Sciences, Faculty of Science, University of CopenhagenTaastrup, Denmark.
authors.forename: Xiangnan
authors.initials: X
authors.lastname: Li
authors.affiliation: National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing Agricultural University Nanjing, China.
authors.forename: Longjing
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen Taastrup, Denmark.
authors.forename: Fulai
authors.initials: F
authors.lastname: Liu
authors.affiliation: National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing Agricultural University Nanjing, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing Agricultural University Nanjing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cai
authors.affiliation: National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing Agricultural University Nanjing, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing Agricultural University Nanjing, China.
authors.forename: Tingbo
authors.initials: T
authors.lastname: Dai
authors.affiliation: National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing Agricultural University Nanjing, China.
authors.forename: Weixing
authors.initials: W
authors.lastname: Cao
authors.affiliation: National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing Agricultural University Nanjing, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Jiang
keywords: isotope; nitrogen; pearling; protein; wheat