title: (1)H, (15)N and (13)C resonance assignments of the C-terminal domain of Vibrio cholerae TolA protein.
abstract: Vibrio cholerae is the bacterial causative agent of the human disease cholera. Non-pathogenic bacterium can be converted to pathogenic following infection by a filamentous phage, CTXΦ, that carries the cholera toxin encoding genes. A crucial step during phage infection requires a direct interaction between the CTXΦ minor coat protein (pIII(CTX)) and the C-terminal domain of V. cholerae TolA protein (TolAIIIvc). In order to get a better understanding of TolA function during the infection process, we have initiated a study of the V. cholerae TolAIII domain by 2D and 3D heteronuclear NMR. With the exception of the His-tag (H123-H128), 97 % of backbone (1)H, (15)N and (13)C resonances were assigned and the side chain assignments for 92 % of the protein were obtained (BMRB deposit with accession number 25689).
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR 7255, CNRS, Aix-Marseille Université, Marseille, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Navarro
authors.affiliation: Institut de Microbiologie de la Méditerranée, FR 3479, CNRS, Aix-Marseille Université, Marseille, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Bornet
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR 7255, CNRS, Aix-Marseille Université, Marseille, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Houot
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR 7255, CNRS, Aix-Marseille Université, Marseille, France.
authors.forename: Roland
authors.initials: R
authors.lastname: Lloubes
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR 7255, CNRS, Aix-Marseille Université, Marseille, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Guerlesquin
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR 7255, CNRS, Aix-Marseille Université, Marseille, France. nouailler@imm.cnrs.fr.
authors.forename: Matthieu
authors.identifier: 0000-0003-2061-466X
authors.initials: M
authors.lastname: Nouailler
mesh_terms: D002772:Cholera Toxin / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D014734:Vibrio cholerae
chemical_list: D002772:Cholera Toxin
keywords: CTXΦ phage; Heteronuclear NMR; TolAIII domain; Vibrio cholerae; pIIICTX