title: Dynamic phosphorylation of Histone Deacetylase 1 by Aurora kinases during mitosis regulates zebrafish embryos development.
abstract: Histone deacetylases (HDACs) catalyze the removal of acetyl molecules from histone and non-histone substrates playing important roles in chromatin remodeling and control of gene expression. Class I HDAC1 is a critical regulator of cell cycle progression, cellular proliferation and differentiation during development; it is also regulated by many post-translational modifications (PTMs). Herein we characterize a new mitosis-specific phosphorylation of HDAC1 driven by Aurora kinases A and B. We show that this phosphorylation affects HDAC1 enzymatic activity and it is critical for the maintenance of a proper proliferative and developmental plan in a complex organism. Notably, we find that Aurora-dependent phosphorylation of HDAC1 regulates histone acetylation by modulating the expression of genes directly involved in the developing zebrafish central nervous system. Our data represent a step towards the comprehension of HDAC1 regulation by its PTM code, with important implications in unravelling its roles both in physiology and pathology.
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Loponte
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Chiara V
authors.initials: CV
authors.lastname: Segré
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Senese
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Miccolo
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Santaguida
authors.affiliation: The FIRC Institute for Molecular Oncology (IFOM), via Adamello 16, 20139 Milan, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Deflorian
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Citro
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Mattoscio
authors.affiliation: The FIRC Institute for Molecular Oncology (IFOM), via Adamello 16, 20139 Milan, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Pisati
authors.affiliation: Department of Medical Biochemistry Max F.Perutz Laboratories Medical University of Vienna, Austria.
authors.forename: Mirjam A
authors.initials: MA
authors.lastname: Moser
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Rosella
authors.initials: R
authors.lastname: Visintin
authors.affiliation: Department of Medical Biochemistry Max F.Perutz Laboratories Medical University of Vienna, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Seiser
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Susanna
authors.initials: S
authors.lastname: Chiocca
mesh_terms: D000107:Acetylation; D000818:Animals; D064127:Aurora Kinases / Q000378:metabolism*; D047108:Embryonic Development; D005809:Genes, Regulator; D056284:Histone Deacetylase 1 / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D008938:Mitosis; D010766:Phosphorylation; D015027:Zebrafish / Q000196:embryology*
chemical_list: D006657:Histones; D064127:Aurora Kinases; D056284:Histone Deacetylase 1