title: In vitro activity of ceftaroline against mecC-positive MRSA isolates.
abstract: Ceftaroline is a new cephalosporin with activity against methicillin-resistant Staphylococcus aureus (MRSA). A collection of 17 clinical and veterinary mecC-positive MRSA isolates was tested to evaluate the in vitro efficacy of ceftaroline against recently emerged mecC-MRSA isolates. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of ceftaroline for the 17 isolates were determined by broth microdilution using the methodology and interpretive criteria of the Clinical and Laboratory Standards Institute (CLSI). Additional susceptibility tests were performed using ceftaroline M.I.C.Evaluator (M.I.C.E.™) strips. All isolates showed susceptibility according to CLSI breakpoints, with MICs of ceftaroline ranging from 0.125mg/L to 0.25mg/L. MBCs were identical or up to a twofold dilution step higher. In conclusion, all tested isolates, from various sources and belonging to several clonal complexes (CCs), but predominantly to CC130, were found to be susceptible to ceftaroline. Ceftaroline could thus be an option for the treatment of mecC-MRSA infections.
authors.affiliation: Labor Staber, Klipphausen, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Armengol-Porta
authors.affiliation: Hospital Juan Ramón Jimenez, Servicio de Microbiología, Huelva, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Tenorio-Abreu
authors.affiliation: Institut Oderland, Frankfurt/Oder, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Bandt
authors.affiliation: Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Dublin, Ireland.
authors.forename: David C
authors.initials: DC
authors.lastname: Coleman
authors.affiliation: Department of Pathology and Wildlife Disease, National Veterinary Institute (SVA), Uppsala, Sweden; Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
authors.forename: Dolores
authors.initials: D
authors.lastname: Gavier-Widen
authors.affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Jena, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Hotzel
authors.affiliation: Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Dublin, Ireland.
authors.forename: Peter
authors.initials: P
authors.lastname: Kinnevey
authors.affiliation: Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Dublin, Ireland.
authors.forename: Alexandros
authors.initials: A
authors.lastname: Lazaris
authors.affiliation: Chemisches und Veterinäruntersuchungsamt Westfalen (AöR), Standort Arnsberg, Arnsberg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Peters
authors.affiliation: Department of Pathology and Wildlife Disease, National Veterinary Institute (SVA), Uppsala, Sweden.
authors.forename: Lena
authors.initials: L
authors.lastname: Rangstrup-Christensen
authors.affiliation: Bavarian Animal Health Service, Poing, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Schlotter
authors.affiliation: Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Dublin, Ireland; Department of Clinical Microbiology, School of Medicine, University of Dublin, Trinity College Dublin, St. James's Hospital, Dublin, Ireland.
authors.forename: Anna C
authors.initials: AC
authors.lastname: Shore
authors.affiliation: Alere Technologies GmbH, Jena, Germany; INFECTOGNOSTICS Research Campus, Jena, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
authors.affiliation: Alere Technologies GmbH, Jena, Germany; INFECTOGNOSTICS Research Campus, Jena, Germany; Institute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany. Electronic address: monecke@rocketmail.com.
authors.forename: Stefan
authors.initials: S
authors.lastname: Monecke
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D000097583:Ceftaroline
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D000097583:Ceftaroline
keywords: Ceftaroline; MRSA; SCCmec XI; Staphylococcus aureus; mecC