title: Establishment of a γ-H2AX foci-based assay to determine biological dose of radon to red bone marrow in rats.
abstract: The biodosimetric information is critical for assessment of cancer risk in populations exposed to high radon. However, no tools are available for biological dose estimation following radon exposure. Here, we established a γ-H2AX foci-based assay to determine biological dose to red bone marrow (RBM) in radon-inhaled rats. After 1-3 h of in vitro radon exposure, a specific pattern of γ-H2AX foci, linear tracks with individual p-ATM and p-DNA-PKcs foci, was observed, and the yield of γ-H2AX foci and its linear tracks displayed a linear dose-response manner in both rat peripheral blood lymphocytes (PBLs) and bone-marrow lymphocytes (BMLs). When the cumulative doses of radon inhaled by rats reached 14, 30 and 60 working level months (WLM), the yields of three types of foci markedly increased in both PBLs and BMLs, and γ-H2AX foci-based dose estimates to RBM were 0.97, 2.06 and 3.94 mGy, respectively. Notably, BMLs displayed a more profound increase of three types of foci than PBLs, and the absorbed dose ratio between BMLs and PBLs was similar between rats exposed to 30 and 60 WLM of radon. Taken together, γ-H2AX foci quantitation in PBLs is able to estimate RBM-absorbed doses with the dose-response curve of γ-H2AX foci after in vitro radon exposure and the ratio of RBM- to PBL-absorbed doses in rats following radon exposure.
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, 200032 Shanghai, China.|Department of Radiology, Ningbo Medical Center, Lihuili Eastern Hospital, Ningbo 315100, Zhejiang, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Division of Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, 201203 Shanghai, China.
authors.forename: Linfeng
authors.initials: L
authors.lastname: He
authors.affiliation: Department of Radiological Health, Institute of Radiation Medicine, Fudan University, 200032 Shanghai, China.
authors.forename: Dunhuang
authors.initials: D
authors.lastname: Fan
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, 200032 Shanghai, China.
authors.forename: Defang
authors.initials: D
authors.lastname: Ding
authors.affiliation: Institute of Modern Physics, Department of Nuclear Science and Technology, Fudan University, 200433 Shanghai, China.
authors.forename: Xufei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, 200032 Shanghai, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, 200032 Shanghai, China.
authors.forename: Xuxia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, 200032 Shanghai, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, 200032 Shanghai, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000396:Air Pollutants, Radioactive / Q000633:toxicity*; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D006657:Histones / Q000032:analysis*; D008214:Lymphocytes / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity*; D011874:Radiometry / Q000379:methods*; D011886:Radon / Q000633:toxicity*; D051381:Rats
chemical_list: D000396:Air Pollutants, Radioactive; D006657:Histones; D009153:Mutagens; D011886:Radon