title: Four New Tirucallane Triterpenoids from the Fruits of Melia azedarach and Their Cytotoxic Activities.
abstract: Four new tirucallane triterpenoids, (21S,23R,24R)-21,23-epoxy-21,24-dihydroxy-25-methoxytirucall-7-en-3-one (2), (3S,21S,23R,24S)-21,23-epoxy-21,25-dimethoxytirucall-7-ene-3,24-diol (8), (21S,23R,24R)-21,23-epoxy-24-hydroxy-21-methoxytirucalla-7,25-dien-3-one (11), and (21S,23R,24R)-21,23-epoxy-21,24-dihydroxytirucalla-7,25-dien-3-one (12), along with 16 known analogues, 1, 3 - 7, 9 - 10, and 13 - 20, were isolated from the fruits of Melia azedarach. Their structures were elucidated by spectroscopic methods including 1D- and 2D-NMR techniques and mass spectrometry. These compounds were evaluated for their cytotoxicities against HepG2 (liver), SGC7901 (stomach), K562 (leukemia), and HL60 (leukemia) cancer cell lines. Compound 20 exhibited potent cytotoxicity against HepG2 and SGC7901 cancer cells with the IC50values of 6.9 and 6.9 μm, respectively.
authors.affiliation: Key Laboratory of Natural Drug Pharmacology in Fujian Province, School of Pharmacy, Fujian Medical University, 88 Jiao Tong Road, Fuzhou, 350004, P. R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Natural Drug Pharmacology in Fujian Province, School of Pharmacy, Fujian Medical University, 88 Jiao Tong Road, Fuzhou, 350004, P. R. China.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Ma
authors.affiliation: Key Laboratory of Natural Drug Pharmacology in Fujian Province, School of Pharmacy, Fujian Medical University, 88 Jiao Tong Road, Fuzhou, 350004, P. R. China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Key Laboratory of Natural Drug Pharmacology in Fujian Province, School of Pharmacy, Fujian Medical University, 88 Jiao Tong Road, Fuzhou, 350004, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Natural Drug Pharmacology in Fujian Province, School of Pharmacy, Fujian Medical University, 88 Jiao Tong Road, Fuzhou, 350004, P. R. China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Natural Drug Pharmacology in Fujian Province, School of Pharmacy, Fujian Medical University, 88 Jiao Tong Road, Fuzhou, 350004, P. R. China.
authors.forename: Zhi-Biao
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: Key Laboratory of Natural Drug Pharmacology in Fujian Province, School of Pharmacy, Fujian Medical University, 88 Jiao Tong Road, Fuzhou, 350004, P. R. China.
authors.forename: Xian-Ming
authors.initials: XM
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Natural Drug Pharmacology in Fujian Province, School of Pharmacy, Fujian Medical University, 88 Jiao Tong Road, Fuzhou, 350004, P. R. China.
authors.forename: Kai-Hui
authors.initials: KH
authors.lastname: Sun
authors.affiliation: Key Laboratory of Natural Drug Pharmacology in Fujian Province, School of Pharmacy, Fujian Medical University, 88 Jiao Tong Road, Fuzhou, 350004, P. R. China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D031586:Melia azedarach / Q000737:chemistry*; D008968:Molecular Conformation; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes; C514393:tirucallane
keywords: 
  Melia azedarach
  ; Cytotoxic activities; Limonoids; Tirucallane triterpenoids