title: High expression of BAG3 predicts a poor prognosis in human medulloblastoma.
abstract: Bcl2-associated athanogene 3 (BAG3), a co-chaperone of the heat shock protein (Hsp) 70, regulates various physiological and pathological processes. However, its role in human medulloblastoma has not been clarified. First of all, the expression of BAG3 was examined in formalin-fixed, paraffin-embedded specimens by immunohistochemical staining. And then, the prognostic role of BAG3 was analyzed in 51 medulloblastoma samples. Finally, the roles of BAG3 in the proliferation, migration, and invasion of Daoy medulloblastoma cell were investigated using a specific short hairpin RNA (shRNA). The expression of BAG3 in medulloblastoma tissues was higher than nontumorous samples. Furthermore, BAG3 overexpression significantly correlated with poor prognosis of patients with medulloblastoma. The overall survival and tumor-free survival in patients with BAG3 low expression were higher than high expression. Univariate and multivariate analysis showed that BAG3 overexpression was an independent prognostic marker for medulloblastoma. After the BAG3 knockdown, the Daoy cells exhibited decreased the ability to proliferate and form neurosphere. The preliminary mechanism study showed that overexpression of BAG3 might facilitate the cell cycle transition from G1 to S phase by modulating the cyclin-dependent kinase 2 (CDK2) and cyclin E expression. Additionally, we found that BAG3 might enhance the medulloblastoma cell migratory and invasive ability. In summary, BAG3 overexpression may regulate the survival and invasive properties of medulloblastoma and may serve as a potential therapy target for medulloblastoma.
authors.affiliation: Department of Neurosurgery, Third Affiliated Hospital, Chongqing Medical University, Chongqing, 401120, China.|Department of Neurosurgery, Rocket Force General Hospital, Chinese People's Liberation Army, Beijing, 100088, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Rocket Force General Hospital, Chinese People's Liberation Army, Beijing, 100088, China.|Department of Healthy Management, Daping Hospital, Third Military Medical University, Chongqing, 400042, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, Third Affiliated Hospital, Chongqing Medical University, Chongqing, 401120, China.|Department of Healthy Management, Daping Hospital, Third Military Medical University, Chongqing, 400042, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Third Affiliated Hospital, Chongqing Medical University, Chongqing, 401120, China.|Department of Neurosurgery, Rocket Force General Hospital, Chinese People's Liberation Army, Beijing, 100088, China.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Third Affiliated Hospital, Chongqing Medical University, Chongqing, 401120, China.|Department of Neurosurgery, Rocket Force General Hospital, Chinese People's Liberation Army, Beijing, 100088, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Third Affiliated Hospital, Chongqing Medical University, Chongqing, 401120, China. zhanggxh68@126.com.|Department of Neurosurgery, Rocket Force General Hospital, Chinese People's Liberation Army, Beijing, 100088, China. zhanggxh68@126.com.
authors.forename: Yundong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003131:Combined Modality Therapy; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008527:Medulloblastoma / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality* / Q000628:therapy; D009367:Neoplasm Staging; D011379:Prognosis; D016896:Treatment Outcome; D047368:Tumor Burden; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C414431:BAG3 protein, human; D014408:Biomarkers, Tumor
keywords: BAG3; Immunohistochemistry; Medulloblastoma; Tumor