title: Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array.
abstract: In this study, HIV-1 Rev response element (RRE) RNA was detected via an Au-coated vertical silicon nanowire electrode array (VSNEA). The VSNEA was fabricated by combining bottom-up and top-down approaches and then immobilized by artificial peptides for the recognition of HIV-1 RRE. Differential pulse voltammetry (DPV) analysis was used to measure the electrochemical response of the peptide-immobilized VSNEA to the concentration and types of HIV-1 RRE RNA. DPV peaks showed linearity to the concentration of RNA with a detection limit down to 1.513 fM. It also showed the clear different peaks to the mutated HIV-1 RRE RNA. The high sensitivity and selectivity of VSNEA for the detection of HIV-1 RRE RNA may be attributed to the high surface-to-volume ratio and total overlap diffusion mode of ions of the one-dimensional nanowire electrodes.
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, Seoul, 03722, South Korea.
authors.forename: Jaehyung
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, Seoul, 03722, South Korea.
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Hong
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, Seoul, 03722, South Korea.
authors.forename: Sanghun
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, Seoul, 03722, South Korea.
authors.forename: Jukwan
authors.initials: J
authors.lastname: Na
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, Seoul, 03722, South Korea.
authors.forename: Ilsoo
authors.initials: I
authors.lastname: Kim
authors.affiliation: Defense Advanced R&D Center, Agency for Defense Development, Daejeon, 34186, South Korea.
authors.forename: Yong-Joon
authors.initials: YJ
authors.lastname: Kwon
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, Seoul, 03722, South Korea. yblim@yonsei.ac.kr.
authors.forename: Yong-Beom
authors.initials: YB
authors.lastname: Lim
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, Seoul, 03722, South Korea. hjc@yonsei.ac.kr.
authors.forename: Heon-Jin
authors.initials: HJ
authors.lastname: Choi
keywords: Biomolecule sensing; HIV-1 RRE RNA; Nanoelectrode; Silicon nanowire