title: Prior Exercise Training Prevent Hyperglycemia in STZ Mice by Increasing Hepatic Glycogen and Mitochondrial Function on Skeletal Muscle.
abstract: Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. We investigated the effect of a prior 30 days voluntary exercise protocol on STZ-diabetic CF1 mice. Glycemia, and the liver and skeletal muscle glycogen, mitochondrial function, and redox status were analyzed up to 5 days after STZ injection. Animals were engaged in the following groups: Sedentary vehicle (Sed Veh), Sedentary STZ (Sed STZ), Exercise Vehicle (Ex Veh), and Exercise STZ (Ex STZ). Exercise prevented fasting hyperglycemia in the Ex STZ group. In the liver, there was decreased on glycogen level in Sed STZ group but not in EX STZ group. STZ groups showed decreased mitochondrial oxygen consumption compared to vehicle groups, whereas mitochondrial H2O2production was not different between groups. Addition of ADP to the medium did not decrease H2O2production in Sed STZ mice. Exercise increased GSH level. Sed STZ group increased nitrite levels compared to other groups. In quadriceps muscle, glycogen level was similar between groups. The Sed STZ group displayed decreased O2consumption, and exercise prevented this reduction. The H2O2production was higher in Ex STZ when compared to other groups. Also, GSH level decreased whereas nitrite levels increased in the Sed STZ compared to other groups. The PGC1 α levels increased in Sed STZ, Ex Veh, and Ex STZ groups. In summary, prior exercise training prevents hyperglycemia in STZ-mice diabetic associated with increased liver glycogen storage, and oxygen consumption by the mitochondria of skeletal muscle implying in increased oxidative/biogenesis capacity, and improved redox status of both tissues. J. Cell. Biochem. 118: 678-685, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Departamento de Bioquímica, ICBS, UFRGS, Programa de Pós Graduação em Ciências Biológicas-Bioquímica, Rua Ramiro Barcelos, 2600 anexo, Porto Alegre, Rio Grande do Sul, CEP 90035-003, Brazil.
authors.forename: Afonso Kopczynski
authors.initials: AK
authors.lastname: de Carvalho
authors.affiliation: Unidade de Ciências da Saúde, Laboratório de Bioquímica e Fisiologia do Exercício Universidade do Extremo Sul Catarinense-UNESC, Av. Universitária, 1105-Bairro Universitário, Criciúma, Santa Catarina, CEP 88806-000, Brazil.
authors.forename: Sabrina
authors.initials: S
authors.lastname: da Silva
authors.affiliation: Unidade de Ciências da Saúde, Laboratório de Bioquímica e Fisiologia do Exercício Universidade do Extremo Sul Catarinense-UNESC, Av. Universitária, 1105-Bairro Universitário, Criciúma, Santa Catarina, CEP 88806-000, Brazil.
authors.forename: Edenir
authors.initials: E
authors.lastname: Serafini
authors.affiliation: Unidade de Ciências da Saúde, Laboratório de Bioquímica e Fisiologia do Exercício Universidade do Extremo Sul Catarinense-UNESC, Av. Universitária, 1105-Bairro Universitário, Criciúma, Santa Catarina, CEP 88806-000, Brazil.
authors.forename: Daniela Roxo
authors.initials: DR
authors.lastname: de Souza
authors.affiliation: Unidade de Ciências da Saúde, Laboratório de Bioquímica e Fisiologia do Exercício Universidade do Extremo Sul Catarinense-UNESC, Av. Universitária, 1105-Bairro Universitário, Criciúma, Santa Catarina, CEP 88806-000, Brazil.
authors.forename: Hemelin Resende
authors.initials: HR
authors.lastname: Farias
authors.affiliation: Unidade de Ciências da Saúde, Laboratório de Bioquímica e Fisiologia do Exercício Universidade do Extremo Sul Catarinense-UNESC, Av. Universitária, 1105-Bairro Universitário, Criciúma, Santa Catarina, CEP 88806-000, Brazil.
authors.forename: Gustavo
authors.initials: G
authors.lastname: de Bem Silveira
authors.affiliation: Unidade de Ciências da Saúde, Laboratório de Bioquímica e Fisiologia do Exercício Universidade do Extremo Sul Catarinense-UNESC, Av. Universitária, 1105-Bairro Universitário, Criciúma, Santa Catarina, CEP 88806-000, Brazil.
authors.forename: Paulo Cesar Lock
authors.initials: PC
authors.lastname: Silveira
authors.affiliation: Unidade de Ciências da Saúde, Laboratório de Bioquímica e Fisiologia do Exercício Universidade do Extremo Sul Catarinense-UNESC, Av. Universitária, 1105-Bairro Universitário, Criciúma, Santa Catarina, CEP 88806-000, Brazil.
authors.forename: Claudio Teodoro
authors.initials: CT
authors.lastname: de Souza
authors.affiliation: Departamento de Bioquímica, ICBS, UFRGS, Programa de Pós Graduação em Ciências Biológicas-Bioquímica, Rua Ramiro Barcelos, 2600 anexo, Porto Alegre, Rio Grande do Sul, CEP 90035-003, Brazil.
authors.forename: Luis Valmor
authors.initials: LV
authors.lastname: Portela
authors.affiliation: Unidade de Ciências da Saúde, Laboratório de Bioquímica e Fisiologia do Exercício Universidade do Extremo Sul Catarinense-UNESC, Av. Universitária, 1105-Bairro Universitário, Criciúma, Santa Catarina, CEP 88806-000, Brazil.
authors.forename: Alexandre Pastoris
authors.initials: AP
authors.lastname: Muller
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000628:therapy; D006943:Hyperglycemia / Q000378:metabolism* / Q000517:prevention & control*; D008112:Liver Glycogen / Q000378:metabolism*; D051379:Mice; D008931:Mitochondria, Muscle / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010805:Physical Conditioning, Animal / Q000502:physiology*
chemical_list: D008112:Liver Glycogen
keywords: GLYCOGEN; HEPATIC; HYPERGLYCEMIA; MUSCLE MITOCHONDRIA; PRIOR EXERCISE