title: Physical Chemical and Biomolecular Methods for the Optimization of Cationic Lipid-Based Lipoplexes In Vitro for the Gene Therapy Applications.
abstract: Preparation and application protocols play a very important role while optimizing the cationic lipid-based lipoplexes in vitro. These protocols serve as the basis for the betterment of the lipoplexes with regard to their successful application in animals and eventually human subjects. Starting from the chemical structures of used cationic lipids (CLs), optimization of the additive inclusions, methods of nanoparticle (lipoplex) formation, presence of blood serum, time intervals of lipoplex incubation, and type of efficiency read-outs in various conditions play important roles in reaching insightful conclusions. Such steps of summarizing protocols and requirements of the pertinent events focus on getting improved lipoplexes for achieving optimal effects in terms of post transfection gene and protein expression. The progression of optimization and efficiency evaluation lead to predictable structure-method-activity relationship with involvement of various feedback principles including physical chemical and biomolecular evaluations before and after the use of lipoplexes in biological systems. This chapter discusses some of the focused strategies for the establishment of lipoplexes for a better post transfection activity with reduced risk of failure.
authors.affiliation: Department of Organic Chemistry, Indian Institute of Science, Bangalore, 560 012, India.|Department of Bioengineering, University of Illinois at Urbana-Champaign, Champaign, IL, 61801, USA.
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Misra
authors.affiliation: Department of Organic Chemistry, Indian Institute of Science, Bangalore, 560 012, India. sb@orgchem.iisc.ernet.in.|Indian Association for the Cultivation of Science, Jadavpur, Kolkata, 700032, India. sb@orgchem.iisc.ernet.in.
authors.forename: Santanu
authors.initials: S
authors.lastname: Bhattacharya
mesh_terms: D002412:Cations / Q000737:chemistry* / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D015316:Genetic Therapy; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D014162:Transfection
chemical_list: D002412:Cations; D008055:Lipids; D008081:Liposomes; D004247:DNA
keywords: Cationic lipids; In vitro culture; Lipoplexes; Liposomes; Plasmid DNA; Transfection