title: Synergistic reversal effect of epithelial-to-mesenchymal transition by miR-223 inhibitor and genistein in gemcitabine-resistant pancreatic cancer cells.
abstract: Emerging studies have demonstrated that EMT phenotype is closely related with tumor progression and drug resistance in a variety of human cancers. Recently, it has been extensively demonstrated that microRNAs (miRNAs) play a pivotal role in regulating EMT. In our previously reports, we have reported that inhibition of miR-223 could reverse EMT phenotype and improve chemotherapeutic drug sensitivity. We also reported that genistein down-regulated miR-223 expression in gemcitabine-resistant (GR) pancreatic cancer cells. Here, we explored whether there was the synergistic effect between miR-223 inhibitor and genistein on cell growth, migration, invasion and reversal of EMT in GR pancreatic cancer. We found that the combination of miR-223 inhibitor and genistein synergistically reduced cell motility and invasion and enhanced gemcitabine sensitivity in GR cells. In addition, we further observed that miR-223 inhibitor and genistein reversed EMT features in GR cells. This study suggests that the combination of miR-223 inhibitor and genistein may be a potential therapeutic strategy for the treatment of pancreatic cancer.
authors.affiliation: The Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Jiangsu Institute of Hematology, The First Affiliated Hospital, Soochow UniversitySuzhou 215123, China; Department of Biochemistry and Molecular Biology, Bengbu Medical CollegeAnhui 233030, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Ma
authors.affiliation: Research Center of Clinical Laboratory Science, Bengbu Medical College Anhui 233030, China.
authors.forename: Fanpeng
authors.initials: F
authors.lastname: Zeng
authors.affiliation: Research Center of Clinical Laboratory Science, Bengbu Medical College Anhui 233030, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Ma
authors.affiliation: Research Center of Clinical Laboratory Science, Bengbu Medical College Anhui 233030, China.
authors.forename: Haijie
authors.initials: H
authors.lastname: Pang
authors.affiliation: Research Center of Clinical Laboratory Science, Bengbu Medical College Anhui 233030, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Fang
authors.affiliation: Department of Biochemistry and Molecular Biology, Bengbu Medical College Anhui 233030, China.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Lian
authors.affiliation: The Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Jiangsu Institute of Hematology, The First Affiliated Hospital, Soochow University Suzhou 215123, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yin
authors.affiliation: Department of Anesthesiology, Shenzhen People's Hospital, 2nd Clinical Medical College of Jinan University Shenzhen 518020, China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Jiangsu Institute of Hematology, The First Affiliated Hospital, Soochow UniversitySuzhou 215123, China; Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical SchoolBoston, MA 02215, USA.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Bengbu Medical College Anhui 233030, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
keywords: EMT; Gemcitabine; genistein; invasion; miR-223; pancreatic cancer