title: Effect of polymorphisms in the CAMKMT gene on growth traits in Ujumqin sheep.
abstract: Our previous genome-wide association study in sheep revealed that OAR3-84073899.1 (SNP31) in intron 8 of the CAMKMT gene was significantly associated with post-weaning gain at the genomic level. Herein, we performed a replication study to investigate single nucleotide polymorphisms (SNPs) within the CAMKMT gene exons, and 1000 bp of the 5'- and 3'-intranslated regions (UTRs) and their associations with growth traits in Ujumqin sheep. Five SNPs were identified through DNA pool sequencing technology: SNP26 in the 5'-UTR, SNP06 in exon 5, SNP07 in exon 8 and SNP27 and SNP28 in the 3'-UTR. Six SNPs, including SNP31 in intron 8, were genotyped in the validation group of 343 Ujumqin sheep, and each SNP was classified into three genotypes. The chi-square test suggested that all the variations were in Hardy-Weinberg equilibrium (P > 0.05) except for SNP28 and SNP31. Linkage disequilibrium analysis showed that SNP07 and SNP31 were strongly linked. An association analysis suggested that SNP06 was significantly associated with chest girth at 6 months of age (P < 0.05). SNP07 exhibited significant correlation with body weight and chest girth at 4 months of age and with body weight, chest girth and chest width at 6 months of age (P < 0.05). SNP27 was highly associated with body weight and chest girth at 4 months of age (P < 0.05), and SNP28 was extremely significantly associated with body weight and chest girth at 4 months of age and with chest girth at 6 months of age (P < 0.01). SNP31 was significantly associated with body weight and shin circumference at 4 months of age and with post-weaning gain (P < 0.05). Association analysis of the combined effect of SNP07 and SNP31 showed significant correlation with body weight and chest girth at four of months of age (P < 0.05) and with body weight and chest girth at 6 months of age (P < 0.05). These results indicate that the SNPs could be used as meritorious and available genetic markers in growth traits breeding and that the CAMKMT gene may be one of the key candidate genes that affect Ujumqin economic traits.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Xiaomeng
authors.identifier: http://orcid.org/0000-0001-8679-6780
authors.initials: X
authors.lastname: Ma
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Long
authors.initials: L
authors.lastname: Guan
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Xuan
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Huihua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Zehu
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Wu
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Ruizao
authors.initials: R
authors.lastname: Liu
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Caiye
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Caihong
authors.initials: C
authors.lastname: Wei
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Fuping
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. lxdu@263.net.
authors.forename: Lixin
authors.initials: L
authors.lastname: Du
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. zhangli@263.net.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001835:Body Weight; D001947:Breeding; D005091:Exons; D005819:Genetic Markers; D005838:Genotype; D015810:Linkage Disequilibrium; D008780:Methyltransferases / Q000235:genetics*; D008957:Models, Genetic; D020125:Mutation, Missense; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D034561:Sheep, Domestic / Q000235:genetics* / Q000254:growth & development*
chemical_list: D005819:Genetic Markers; D008780:Methyltransferases; C038999:calmodulin-lysine methyltransferase
keywords: Post-GWAS; SNP; body weight; chest girth; gene validation