title: Protocols for Efficient Differentiation of Hair Follicle-Associated Pluripotent (HAP) Stem Cells to Beating Cardiac Muscle Cells.
abstract: We have previously demonstrated that the nestin-expressing cells from the upper part of the hair follicle can differentiate to neurons and other cell types. We have termed these cells as hair-associated-pluripotent (HAP) stem cells. In the present chapter, we describe methods for HAP stem cells to differentiate to beating cardiac muscle cells. The mouse vibrissa hair follicle was divided into three parts (upper, middle, and lower), and each part was suspended separately in DMEM containing 10 % fetal bovine serum (FBS). All three parts of hair follicle differentiate to neurons, glial cells, keratinocytes, smooth muscle cells, and cardiac muscle cells. The differentiation potential to cardiac muscle is greatest in the upper part of the follicle. Hair spheres comprised of HAP stem cells formed from the upper part of vibrissa hair follicle can differentiate to cardiac muscle cells.
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Minami Ward, Sagamihara, 252-0374, Japan.
authors.forename: Masateru
authors.initials: M
authors.lastname: Yashiro
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Minami Ward, Sagamihara, 252-0374, Japan.
authors.forename: Sumiyuki
authors.initials: S
authors.lastname: Mii
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Minami Ward, Sagamihara, 252-0374, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Aki
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Minami Ward, Sagamihara, 252-0374, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hamada
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Minami Ward, Sagamihara, 252-0374, Japan.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Arakawa
authors.affiliation: Department of Physiology, Kitasato University School of Medicine, Minami Ward, Sagamihara, 252-0374, Japan.|Department of Cellular & Molecular Physiology, Kitasato University Graduate School Medical Science, Sagamihara, Japan.
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Kawahara
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, CA, 92111, USA.|Department of Surgery, University of California, San Diego, San Diego, CA, 92103, USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Minami Ward, Sagamihara, 252-0374, Japan. yasuyukiamoh@aol.com.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Amoh
mesh_terms: D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D005455:Fluorescent Antibody Technique; D018859:Hair Follicle / Q000166:cytology*; D016130:Immunophenotyping; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D014738:Vibrissae
chemical_list: D015415:Biomarkers
keywords: Cardiac muscle cells; Differentiation; HAP stem cell; Hair follicle; Hair spheres; Vibrissa