title: Effects of ATP and adenosine on contraction amplitude of rat soleus muscle at different temperatures.
abstract: The aim of this study was to evaluate the effects of adenosine 5'-triphosphate (ATP) and adenosine on the contractility of mammalian skeletal muscle under hypothermic conditions.
  
  
  
  Contractions of isolated rat soleus muscle were induced by either electrical stimulation (ES) or carbachol at physiological temperatures (37°C) and hypothermic conditions (30-14°C) and recorded in the presence of ATP, adenosine, suramin, and 8-(p-sulfophenyl)-theophylline (8-SPT).
  
  
  
  At 37°C, incubation of the muscles with ATP inhibited ES-induced contractions; the inhibitory effect of ATP disappeared at 14°C. Adenosine inhibited ES-induced contractions at all temperature levels; 8-SPT fully prevented the action of adenosine. ATP and adenosine did not significantly affect carbachol-induced contractions at 37°C, while at lower temperatures ATP potentiated them. Suramin fully prevented effects of ATP.
  
  
  
  ATP is involved in both pre- and postsynaptic regulation of rat soleus muscle contractility, and these processes are significantly more pronounced at low temperatures. Muscle Nerve 55: 417-423, 2017.
authors.affiliation: Department of Pharmacology, Pharmacognosy, and Botany, Kazan State Medical University, 49 Butlerov Street, Kazan, 420012, Russia.
authors.forename: Ayrat U
authors.initials: AU
authors.lastname: Ziganshin
authors.affiliation: Department of Pharmacology, Pharmacognosy, and Botany, Kazan State Medical University, 49 Butlerov Street, Kazan, 420012, Russia.
authors.forename: Adel E
authors.initials: AE
authors.lastname: Khairullin
authors.affiliation: A.E. Arbuzov Institute of Organic and Physical Chemistry, Russian Academy of Sciences, Kazan, Russia.|Department of Applied Ecology, Kazan Federal University, Kazan, Russia.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Zobov
authors.affiliation: Department of Fundamental and Clinical Pharmacology, Kazan Federal University, Kazan, Russia.
authors.forename: Liliya E
authors.initials: LE
authors.lastname: Ziganshina
authors.affiliation: Department of Fundamental and Clinical Pharmacology, Kazan Federal University, Kazan, Russia.
authors.forename: Azat I
authors.initials: AI
authors.lastname: Gabdrakhmanov
authors.affiliation: Department of Pharmacology, Pharmacognosy, and Botany, Kazan State Medical University, 49 Butlerov Street, Kazan, 420012, Russia.|Aortic Institute at Yale-New Haven Hospital, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: Bulat A
authors.initials: BA
authors.lastname: Ziganshin
authors.affiliation: Department of Medical and Biological Physics, Kazan State Medical University, Kazan, Russia.|Department of Television and Multimedia Systems, Kazan National Research Technical University, Kazan, Russia.
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Grishin
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D018679:Cholinergic Agonists / Q000494:pharmacology; D004558:Electric Stimulation; D007035:Hypothermia / Q000139:chemically induced; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000502:physiology*; D018733:Nicotinic Antagonists / Q000494:pharmacology; D058915:Purinergic P1 Receptor Antagonists / Q000494:pharmacology; D058919:Purinergic P2 Receptor Antagonists / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013498:Suramin / Q000494:pharmacology; D013696:Temperature; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology; D014403:Tubocurarine / Q000494:pharmacology
chemical_list: D018679:Cholinergic Agonists; D018733:Nicotinic Antagonists; D058915:Purinergic P1 Receptor Antagonists; D058919:Purinergic P2 Receptor Antagonists; D013498:Suramin; C031433:8-(4-sulfophenyl)theophylline; D000255:Adenosine Triphosphate; D002217:Carbachol; D013806:Theophylline; D000241:Adenosine; D014403:Tubocurarine
keywords: ATP; P2 receptors; adenosine; hypothermia; skeletal muscle; suramin