title: Identification of a Novel Complex between the Nucleoprotein and PA(1-27) of Influenza A Virus Polymerase.
abstract: The structure of the ribonucleoprotein complexes is crucial to viral transcription and replication of influenza virus, but association of the nucleoprotein (NP) with the polymerase remains to be characterized at the molecular level. Here, we identify a peptide of the polymerase acidic subunit PA(1-27) that associates with NP. Docking and molecular dynamics simulations suggest a similar NP binding site with PA(1-27) and PA(1-186). The PA(1-27)-NP complex is characterized by surface plasmon resonance and fluorescence using recombinant NP proteins and by pull-down assays in infected cells. The PA(1-27)-NP complex may have a role in the final steps of transcription and replication.
authors.affiliation: Paris Saclay University , UR892, INRA, 78350 Jouy en Josas, France.
authors.forename: Jasmina
authors.initials: J
authors.lastname: Vidic
authors.affiliation: UMS IPSIT-Intermol, University Paris Sud, Paris Saclay University , 92296 Châtenay-Malabry, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Noiray
authors.affiliation: Paris Saclay University , UR892, INRA, 78350 Jouy en Josas, France.|Department of Biochemistry and Biophysics, University of Kalyani , Kalyani, India.
authors.forename: Angshuman
authors.initials: A
authors.lastname: Bagchi
authors.affiliation: Paris Saclay University , UR892, INRA, 78350 Jouy en Josas, France.
authors.forename: Anny
authors.initials: A
authors.lastname: Slama-Schwok
mesh_terms: D000818:Animals; D004285:Dogs; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry* / Q000502:physiology; D061985:Madin Darby Canine Kidney Cells; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D046912:Multiprotein Complexes / Q000737:chemistry; D019590:Nucleocapsid Proteins; D054730:Protein Interaction Domains and Motifs; D021122:Protein Subunits / Q000737:chemistry; D016601:RNA-Binding Proteins / Q000737:chemistry*; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D020349:Surface Plasmon Resonance; D014758:Viral Core Proteins / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D046912:Multiprotein Complexes; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; C061290:PA protein, influenza viruses; D021122:Protein Subunits; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D014758:Viral Core Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase