title: Reductive Transformation of p-chloronitrobenzene in the upflow anaerobic sludge blanket reactor coupled with microbial electrolysis cell: performance and microbial community.
abstract: A microbial electrolysis cell (MEC) combined with an upflow anaerobic sludge blanket (UASB) reactor was operated to degrade p-chloronitrobenzenes (p-ClNB) effectively. The results indicated that p-ClNB was transformed to p-chloroaniline (p-ClAn) and then reduced via dechlorination pathways. In the MEC-UASB coupled system, p-ClNB, p-ClAn removal efficiency and dechlorination efficiency reached 99.63±0.37%, 40.39±9.26% and 32.16±8.12%, respectively, which was significantly improved in comparison with the control UASB system. In addition, the coupled system could maintain appropriate pH and promote anaerobic sludge granulation to exert a positive effect on reductive transformation of p-ClNB. PCR-DGGE experiment and 454 pyrophosphate sequencing analysis indicated that applied voltage would significantly influence the succession of microbial community and promote oriented enrichment of the functional bacteria, which could be the underlying reasons for the improved performance. This study demonstrated that MEC-UASB coupled system had a promising application prospect to remove the recalcitrant pollutants effectively.
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China; Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou 310058, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Shao
authors.affiliation: Department of Chemistry and Environmental Science, New Jersey Institute of Technology University, NJ 07102, United States.
authors.forename: Mengyan
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China; Equipment Engineering Company, China United Engineering Corporation, Hangzhou 310052, China.
authors.forename: Kaituo
authors.initials: K
authors.lastname: Gao
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jin
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China; Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou 310058, China. Electronic address: felix79cn@hotmail.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000693:Anaerobiosis; D000814:Aniline Compounds / Q000737:chemistry; D019149:Bioreactors / Q000382:microbiology*; D009578:Nitrobenzenes / Q000737:chemistry; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D000814:Aniline Compounds; D009578:Nitrobenzenes; D012722:Sewage; C010407:4-chloronitrobenzene; C004658:4-chloroaniline
keywords: Chloronitrobenzenes (ClNBs); Dechlorination; Microbial community; Microbial electrolysis cell (MEC); Upflow anaerobic sludge blanket (UASB)