title: A highly selective hydrophilic sorbent for enrichment of N-linked glycopeptides.
abstract: Selective enrichment and purification of N-glycopeptides from complex biological samples is often a necessary step for effective identification of low abundance glycopeptides. A polyethylenimine functionalized sorbent described here demonstrates good enrichment ability and excellent selectivity towards acidic glycopeptides (e.g. sialylated glycopeptides), even at 1:3000 mass ratio of target glycopeptides and non-glycopeptides, which is much more superior to commercial ZIC-HILIC and other reported sorbents. It also shows effective enrichment for neutral glycopeptides (IgG). Another desirable feature of such sorbent is its large binding capacity (∼220mg/g for fetuin).
authors.affiliation: School of Pharmacy, East-China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Fu
authors.affiliation: School of Pharmacy, East-China University of Science and Technology, Shanghai 200237, China.
authors.forename: Feifang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, East-China University of Science and Technology, Shanghai 200237, China. Electronic address: bcyang@ecust.edu.cn.
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Yang
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Long
authors.initials: L
authors.lastname: Yu
authors.affiliation: School of Pharmacy, East-China University of Science and Technology, Shanghai 200237, China; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D060748:Fetuins / Q000378:metabolism; D006020:Glycopeptides / Q000032:analysis* / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D011094:Polyethyleneimine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D060748:Fetuins; D006020:Glycopeptides; D012822:Silicon Dioxide; D011094:Polyethyleneimine
keywords: Glycopeptides; Hydrophilic interaction chromatography; Hyperbranched; Polyethylenimine; Solid phase extraction