title: Parishin from Gastrodia elata Extends the Lifespan of Yeast via Regulation of Sir2/Uth1/TOR Signaling Pathway.
abstract: Parishin is a phenolic glucoside isolated from Gastrodia elata, which is an important traditional Chinese medicine; this glucoside significantly extended the replicative lifespan of K6001 yeast at 3, 10, and 30 μM. To clarify its mechanism of action, assessment of oxidative stress resistance, superoxide dismutase (SOD) activity, malondialdehyde (MDA), and reactive oxygen species (ROS) assays, replicative lifespans of sod1, sod2, uth1, and skn7 yeast mutants, and real-time quantitative PCR (RT-PCR) analysis were conducted. The significant increase of cell survival rate in oxidative stress condition was observed in parishin-treated groups. Silent information regulator 2 (Sir2) gene expression and SOD activity were significantly increased after treating parishin in normal condition. Meanwhile, the levels of ROS and MDA in yeast were significantly decreased. The replicative lifespans of sod1, sod2, uth1, and skn7 mutants of K6001 yeast were not affected by parishin. We also found that parishin could decrease the gene expression of TORC1, ribosomal protein S26A (RPS26A), and ribosomal protein L9A (RPL9A) in the target of rapamycin (TOR) signaling pathway. Gene expression levels of RPS26A and RPL9A in uth1, as well as in uth1, sir2 double mutants, were significantly lower than those of the control group. Besides, TORC1 gene expression in uth1 mutant of K6001 yeast was inhibited significantly. These results suggested that parishin exhibited antiaging effects via regulation of Sir2/Uth1/TOR signaling pathway.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Lin
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou, China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou, China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Weng
authors.affiliation: Department of Nanobiology, Graduate School of Advanced Integration Science, Chiba University, Chiba 263-8522, Japan.
authors.forename: Akira
authors.identifier: 0000-0002-2300-7227
authors.initials: A
authors.lastname: Matsuura
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Xiang
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qi
mesh_terms: D031668:Gastrodia / Q000737:chemistry*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D005960:Glucosides; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D008315:Malondialdehyde; D013482:Superoxide Dismutase