title: Angiogenic Synergistic Effect of Adipose-Derived Stromal Cell Spheroids with Low-Level Light Therapy in a Model of Acute Skin Flap Ischemia.
abstract: Human adipose-derived mesenchymal stem cells (hASCs) are an attractive cell source for tissue engineering. However, one obstacle to this approach is that the transplanted hASC population can decline rapidly in the recipient tissue. The aim of this study was to investigate the effects of low-level light therapy (LLLT) on transplanted spheroid hASCs in skin flaps of mice. hASCs were cultured in monolayers or spheroids. LLLT, hASCs, spheroids and spheroids transplanted with LLLT were applied to the skin flaps. Healing of the skin flaps was assessed by gross evaluation and by hematoxylin and eosin staining and elastin van Gieson staining. Compared with the spheroid group, skin flap healing was enhanced in the spheroid + LLLT group, including the neovascularization and regeneration of skin appendages. The survival of hASCs was enhanced by decreased apoptosis of hASCs in the skin flaps of the spheroid + LLLT group. The secretion of growth factors was stimulated in the spheroid + LLLT group compared with the ASC and spheroid groups. These data suggest that LLLT was an effective biostimulator of spheroid hASCs in the skin flaps, enhancing the survival of hASCs and stimulating the secretion of growth factors.
authors.forename: In-Su
authors.initials: IS
authors.lastname: Park
authors.forename: Phil-Sang
authors.initials: PS
authors.lastname: Chung
authors.forename: Jin Chul
authors.initials: JC
authors.lastname: Ahn
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007511:Ischemia / Q000532:radiotherapy*; D028022:Low-Level Light Therapy; D051379:Mice; D018919:Neovascularization, Physiologic; D012867:Skin / Q000473:pathology* / Q000528:radiation effects*; D018874:Spheroids, Cellular / Q000166:cytology*; D033581:Stem Cell Transplantation; D017154:Stromal Cells / Q000166:cytology; D054457:Tissue Scaffolds
chemical_list: D043925:Angiogenesis Inducing Agents; D036341:Intercellular Signaling Peptides and Proteins