title: Characteristics of Lymphoproliferative Disorders with More Than One Aberrant Cell Population as Detected by 10-Color Flow Cytometry.
abstract: We have evaluated the frequency of lymphoproliferative disorders with more than one aberrant population of monotypic B-cells detected during routine hematopathological diagnostics.
  
  
  
  2600 samples peripheral (blood, bone marrow, fine-needle aspirate, lymph node, and pleural fluid cell suspensions) were analyzed using a 10-color B-cell panel and a 10-color T-cell panel. A 10-color plasma cell/lymphoplasmacytic panel was performed when appropriate.
  
  
  
  790/2600 samples (30%) showed at least one aberrant B-cell population and 27(1%) showed an aberrant T-cell population. 41/790 samples (5.1%) showed two aberrant B-cell populations. Thirteen patients had two B-cell populations with different surface immunoglobulin restriction (one kappa+ and one lambda+), most with B-cell chronic lymphocytic leukemia-related phenotype. Five cases showed two B-cell populations with the same light chain restriction but distinctly different immunophenotypes. In 23 cases, two populations had the same light chain restriction and differed by expression of one or 2 markers, thus, a possibility of intraclonal differentiation could not be excluded. Cases with possible intraclonal differentiation had a significantly higher proportion of aberrant B-cells than those with two coexisting aberrant B-cell populations (49.9% vs. 27.7%, p = 0.008). In only one sample one population of clonal B-cell and one clonal T-cell population with large granular lymphocyte related phenotype were found.
  
  
  
  Using our panels 5.1% of cases with lymphoproliferative disorder-associated aberrant findings show two aberrant (clonal) lymphoid and/or plasma cell populations. © 2016 International Clinical Cytometry Society.
authors.affiliation: Department of Pathology and Laboratory Medicine, the Ottawa Hospital and Eastern Ontario Regional Laboratory Association and University of Ottawa, ON, Canada.|Department of Laboratory Hematology, Flow Cytometry Laboratory, Laboratory Medicine Program, University Health Network, Toronto, ON, Canada.
authors.forename: Talal
authors.initials: T
authors.lastname: Mahdi
authors.affiliation: Department of Laboratory Hematology, Flow Cytometry Laboratory, Laboratory Medicine Program, University Health Network, Toronto, ON, Canada.
authors.forename: Amr
authors.initials: A
authors.lastname: Rajab
authors.affiliation: Department of Pathology and Laboratory Medicine, the Ottawa Hospital and Eastern Ontario Regional Laboratory Association and University of Ottawa, ON, Canada.
authors.forename: Ruth
authors.initials: R
authors.lastname: Padmore
authors.affiliation: Department of Laboratory Hematology, Flow Cytometry Laboratory, Laboratory Medicine Program, University Health Network, Toronto, ON, Canada.
authors.forename: Anna
authors.initials: A
authors.lastname: Porwit
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000473:pathology; D002999:Clone Cells / Q000473:pathology; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010950:Plasma Cells / Q000473:pathology; D013601:T-Lymphocytes / Q000473:pathology
keywords: B-cell; bi-clonal; flow cytometry; lymphoproliferative disorders