title: [Change in plasma nesfatin-1 concentration within high-fat diet induced nonalcoholic fatty liver disease rat models].
abstract: OBJECTIVE
  To investigate the change of plasma nesfatin-1 concentration in a nonalcoholic fatty liver disease rat model induced by high-fat diet, and explore its effect on the dysfunction of glucose and lipid metabolism.
  
  
  METHODS
  The nonalcoholic fatty liver disease rat model was established through introduction of a high-fat diet, and four weeks later, the intraperitoneal glucose tolerance test was conducted. Serum concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), total cholesterol (TC) triglyceride (TG), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) were detected using chemiluminescence technique. The plasma levels of nesfatin-1, leptin, and insulin (INS) were measured via enzyme-linked immunosorbent assay (ELISA), the histological changes of the liver was observed via HE staining, and the protein expressions of beta-catenin, p-beta-catenin and cyclin D1 in the liver were detected using western blot and compared with beta-actin.
  
  
  RESULTS
  The bodyweight, liver weight, liver index, and area under the curve of the intraperitoneal glucose tolerance test were all higher in the model rats than those in the controls. Compared with the control, serum concentrations of ALT, TBIL, IBIL, TC and LDL-C in the model rats were all increased. The plasma nesfatin-1 level was increased in model rats while the plasma concentrations of leptin and insulin were decreased, and a negative correlation was found between the plasma concentration of nesfatin-1 and leptin. Inflammation and hepatocyte steatosis were detected in the livers of model rats, and the protein expression of cyclinD1 was upregulated while the phosphorylation of beta-catenin was decreased in the livers of the model rats.
  
  
  CONCLUSIONS
  Post-creation of nonalcoholic fatty liver disease rat models through high fat diets, changes were observed in plasma nesfatin-1 concentration, perhaps a vital part of glucose and lipid metabolism dysfunction.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chen
authors.forename: Yayun
authors.initials: Y
authors.lastname: Xu
authors.forename: Jinfang
authors.initials: J
authors.lastname: Ge
authors.forename: Xiongwen
authors.initials: X
authors.lastname: Lü
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001835:Body Weight; D002135:Calcium-Binding Proteins / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D019938:Cyclin D1 / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000097:blood*; D059305:Diet, High-Fat; D004797:Enzyme-Linked Immunosorbent Assay; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D020738:Leptin / Q000097:blood; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000097:blood*; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood*; D000080866:Nucleobindins; D051381:Rats; D014280:Triglycerides / Q000097:blood; D051176:beta Catenin / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; C530106:Ccnd1 protein, rat; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; C495268:Ctnnb1 protein, rat; D004268:DNA-Binding Proteins; D007328:Insulin; D020738:Leptin; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D014280:Triglycerides; D051176:beta Catenin; D019938:Cyclin D1; D002784:Cholesterol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin