title: Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles.
abstract: The hair follicle undergoes lifelong cycling and growth. Previous studies have been focused on epithelial stem cells in the hair follicles. Neural crest stem cells (NCSCs) are pluripotent cells that can persist in adult tissues. We have previously demonstrated that human NCSCs can be isolated from hair follicles. Here, we present a protocol to isolate NCSCs from human hair follicles based on their specific surface-marker expression of CD271/HNK1 or CD271/CD49D (alpha4 integrin). NCSCs can be expanded in the culture as neural spheres or attached cells.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104, USA.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104, USA. xug@mail.med.upenn.edu.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xu
mesh_terms: D015415:Biomarkers; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005434:Flow Cytometry; D018859:Hair Follicle / Q000166:cytology*; D006801:Humans; D009432:Neural Crest / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D015415:Biomarkers
keywords: Bulge; Hair follicle; Neural crest stem cells; Protocol