title: A proof-of-concept clinical study examining the NRF2 activator sulforaphane against neutrophilic airway inflammation.
abstract: Sulforaphane (SFN), a naturally occurring isothiocyanate found in cruciferous vegetables, is implicated as a possible therapy for airway inflammation via induction of the transcription factor NF-E2-related factor 2 (NRF2). In this proof-of-concept clinical study, we show that supplementation of SFN with broccoli sprout homogenate in healthy human subjects did not induce expression of antioxidant genes or protect against neutrophilic airway inflammation in an ozone-exposure model. Therefore, dietary sulforaphane supplementation is not a promising candidate for larger scale clinical trials targeting airway inflammation.
  
  
  
  NCT01625130 . Registered 19 June, 2012.
authors.affiliation: UNC Center for Environmental Medicine, Asthma, & Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Charity G
authors.initials: CG
authors.lastname: Duran
authors.affiliation: UNC Center for Environmental Medicine, Asthma, & Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Allison J
authors.initials: AJ
authors.lastname: Burbank
authors.affiliation: UNC Center for Environmental Medicine, Asthma, & Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Katherine H
authors.initials: KH
authors.lastname: Mills
authors.affiliation: UNC Center for Environmental Medicine, Asthma, & Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Heather R
authors.initials: HR
authors.lastname: Duckworth
authors.affiliation: UNC Center for Environmental Medicine, Asthma, & Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Maria M
authors.initials: MM
authors.lastname: Aleman
authors.affiliation: Department of Physical Therapy, Methodist University, Fayetteville, NC, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Kesic
authors.affiliation: UNC Center for Environmental Medicine, Asthma, & Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: David B
authors.initials: DB
authors.lastname: Peden
authors.affiliation: Department of Biostatistics, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Yinghao
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Biostatistics, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhou
authors.affiliation: UNC Center for Environmental Medicine, Asthma, & Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. michelle_hernandez@med.unc.edu.|Division of Allergy, Immunology & Rheumatology, UNC School of Medicine, 104 Mason Farm Road, CB #7310, Chapel Hill, NC, 27599-7310, USA. michelle_hernandez@med.unc.edu.
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Hernandez
mesh_terms: D000293:Adolescent; D000328:Adult; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D001937:Brassica / Q000737:chemistry; D018592:Cross-Over Studies; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D017879:Isothiocyanates / Q000302:isolation & purification / Q000627:therapeutic use*; D007960:Leukocyte Disorders / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D051267:NF-E2-Related Factor 2 / Q000819:agonists* / Q000378:metabolism; D020556:Neutrophil Infiltration / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010126:Ozone; D008517:Phytotherapy; D010946:Plants, Medicinal; D011014:Pneumonia / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D013454:Sulfoxides; D055815:Young Adult
chemical_list: D000893:Anti-Inflammatory Agents; D017879:Isothiocyanates; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D013454:Sulfoxides; D010126:Ozone; C016766:sulforaphane
keywords: Airway inflammation; Antioxidants; NRF2; Ozone; Sulforaphane