title: A Redox-Nucleophilic Dual-Reactable Probe for Highly Selective and Sensitive Detection of H2S: Synthesis, Spectra and Bioimaging.
abstract: Hydrogen sulfide (H2S) is an important signalling molecule with multiple biological functions. The reported H2S fluorescent probes are majorly based on redox or nucleophilic reactions. The combination usage of both redox and nucleophilic reactions could improve the probe's selectivity, sensitivity and stability. Herein we report a new dual-reactable probe with yellow turn-on fluorescence for H2S detection. The sensing mechanism of the dual-reactable probe was based on thiolysis of NBD (7-nitro-1,2,3-benzoxadiazole) amine (a nucleophilic reaction) and reduction of azide to amine (a redox reaction). Compared with its corresponding single-reactable probes, the dual-reactable probe has higher selectivity and fluorescence turn-on fold with magnitude of multiplication from that of each single-reactable probe. The highly selective and sensitive properties enabled the dual-reactable probe as a useful tool for efficiently sensing H2S in aqueous buffer and in living cells.
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology (BUCT), Beijing, China.
authors.forename: Changyu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Elemento-Organic Chemistry, Department of Chemical Biology, National Engineering Research Center of Pesticide (Tianjin), Nankai University, Tianjin, China.|Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin, China.
authors.forename: Runyu
authors.initials: R
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Elemento-Organic Chemistry, Department of Chemical Biology, National Engineering Research Center of Pesticide (Tianjin), Nankai University, Tianjin, China.|Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin, China.
authors.forename: Longhuai
authors.initials: L
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology (BUCT), Beijing, China.
authors.forename: Bingjie
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Laboratory of Elemento-Organic Chemistry, Department of Chemical Biology, National Engineering Research Center of Pesticide (Tianjin), Nankai University, Tianjin, China.|Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xi
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology (BUCT), Beijing, China.
authors.forename: Long
authors.initials: L
authors.lastname: Yi
mesh_terms: D006862:Hydrogen Sulfide / Q000032:analysis*; D057230:Limit of Detection; D015335:Molecular Probes / Q000378:metabolism*; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D015335:Molecular Probes; D006862:Hydrogen Sulfide