title: Nestin-Based Reporter Transgenic Mouse Lines.
abstract: Nestin expression marks stem and progenitor cells of the neural lineage. Transgenic mouse lines, originally generated to identify neural stem cells, can also help to identify, track, and isolate stem and progenitor cells in a range of tissues of the ectodermal, endodermal, and mesodermal origin. Here, we describe the generation of transgenic mouse lines expressing fluorescent proteins (FP) under the control of critical regulatory elements of the nestin gene and their use for identifying and analyzing adult stem and progenitor cells in various tissues.
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA.
authors.forename: John
authors.initials: J
authors.lastname: Mignone
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Peunova
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA. enik@csh.edu.
authors.forename: Grigori
authors.initials: G
authors.lastname: Enikolopov
mesh_terms: D000818:Animals; D015870:Gene Expression; D017930:Genes, Reporter; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D064231:Nestin / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D013234:Stem Cells / Q000378:metabolism
chemical_list: D064231:Nestin; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
keywords: Hair-follicle-associated pluripotent (HAP); Immunocytochemistry; Nestin; Nestin-GFP; Reporter lines; Stem cells; Transgenic animals