title: Effect of Areca Nut onHelicobacter pylori-Induced Gastric Diseases in Mice.
abstract: Areca nut (AN) chewing is a habit in many countries in Central, Southern, and Southeast Asia. It is strongly associated with the occurrence of oral, pharyngeal, and esophageal cancer as well as systemic inflammation. However, the association between AN intake and the development of gastric lesions has not yet been identified. The aim of this study was to investigate the effect of AN on gastric diseases using a mouse model forHelicobacter pyloriinfection. We studied four groups of mice: those fed a normal diet (ND), those fed a diet containing 2.5% AN (AD), those fed ND and infected withH. pyloriPMSS1 strain (ND/HP), and those fed AD and infected withH. pyloriPMSS1 strain (AD/HP). Food intake and body weight were monitored weekly during the experiments. At 10 weeks, the mice were sacrificed, and the stomach weight,H. pyloricolonization, and gastric inflammation were evaluated. The stomach weight had increased significantly in the ND/HP and AD/HP groups along with increases inH. pyloricolonization; however, there was no significant difference between these two groups with respect to stomach weight and colonization. On histological grading, mononuclear cell infiltration was severer in the AD/HP group than in the ND/HP group. These data suggest that chronic gastric inflammation was aggravated by AN treatment in the mice withH. pylori-induced gastric lesions. Furthermore, as previously suggested, this animal model is useful to determine the effect of potential carcinogens on gastric lesions induced by H. pylori infection.
authors.affiliation: Department of Dentistry, The Graduate School, Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.
authors.forename: Jinwook
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.
authors.forename: Niluka D
authors.initials: ND
authors.lastname: Gunawardhana
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Sungil
authors.initials: S
authors.lastname: Jang
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Yun Hui
authors.initials: YH
authors.lastname: Choi
authors.affiliation: Department of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Peradeniya, Peradeniya, Sri Lanka.
authors.forename: Rasika P
authors.initials: RP
authors.lastname: Illeperuma
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Aeryun
authors.initials: A
authors.lastname: Kim
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Hanfu
authors.initials: H
authors.lastname: Su
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Youngmin A
authors.initials: YA
authors.lastname: Hong
authors.affiliation: Department of Dental Hygiene, Jeonju Kijeon College, Jeonju 54989, Republic of Korea.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.
authors.forename: Jinmoon
authors.initials: J
authors.lastname: Kim
authors.affiliation: New Drug Targets Laboratory, School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea.
authors.forename: Da-Woon
authors.initials: DW
authors.lastname: Jung
authors.affiliation: Department of Dentistry, The Graduate School, Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.
authors.forename: In-Ho
authors.initials: IH
authors.lastname: Cha
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Bak
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul 03722, Republic of Korea.|Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, P.R. China.
authors.forename: Jeong-Heon
authors.initials: JH
authors.lastname: Cha
mesh_terms: D000818:Animals; D001114:Areca / Q000737:chemistry*; D016481:Helicobacter Infections / Q000473:pathology*; D016480:Helicobacter pylori; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009754:Nuts; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D013270:Stomach / Q000187:drug effects / Q000473:pathology; D013272:Stomach Diseases / Q000473:pathology*
chemical_list: D010936:Plant Extracts
keywords: Areca nut; Helicobacter pylori; gastric diseases