title: Loss of miR-26a-5p promotes proliferation, migration, and invasion in prostate cancer through negatively regulating SERBP1.
abstract: The biological role of miR-26a involved in the carcinogenesis of prostate cancer (PC) has been controversial. Besides, the underlying mechanism by which miR-26a plays a role in PC has been unclear. To investigate the role of miR-26a-5p in the PC, miR-26a-5p was detected and statistically analyzed in clinical PC tissues and a panel of PC cell lines. Using bioinformatics analysis, we found that serpine1 messenger RNA (mRNA) binding protein 1 (SERBP1) was a potential downstream target of miR-26a-5p. Using luciferase reporter and western blot, we identified that miR-26a-5p negatively regulated SERBP1 on the PC cell line level. It was confirmed that miR-26a-5p was markedly downregulated in PC tissues compared with normal controls whose reduced expression was significantly associated with metastasis and poor overall prognosis and found that miR-26a-5p was able to prevent proliferation and motility of PC cells in vitro. Additionally, SERBP1 was identified as a downstream target of miR-26a-5p. Moreover, it was observed that SERBP1 was markedly upregulated in prostate cancer tissues and was significantly associated with tissue metastasis and Gleason score. Taken together, our results for the first time demonstrate that the loss of miR-26a-5p promotes proliferation, migration, and invasion through targeting SERBP1 in PC, supporting the tumor-suppressing role of miR-26a-5p in PC.
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Guo
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.
authors.forename: Abai
authors.initials: A
authors.lastname: Xu
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.
authors.forename: Binshen
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China. doctor_ywen@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wen
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D017063:Outcome Assessment, Health Care / Q000379:methods / Q000706:statistics & numerical data; D011379:Prognosis; D016016:Proportional Hazards Models; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D016601:RNA-Binding Proteins; C528163:MIRN26 microRNA, human; C428129:SERBP1 protein, human
keywords: Metastasis; Prognosis; Proliferation; Prostate cancer; SERBP1; miR-26a-5p