title: CD4 + T Cells from Lymph Nodes Involved by Hodgkin's Disease Showing Response in Autologous Mixed Lymphocyte Reaction, are Polyclonal.
abstract: Thirteen CD4 + T cell clones (TCCs) showing autologous mixed lymphocyte reaction (AMLR) and 12 CD4 + AMLR-negative TCCs derived from involved lymph nodes (LN) of 4 untreated patients with newly-diagnosed Hodgkin's disease (HD) were analyzed for some functional activities and for T cell receptor (TCR) gamma and beta gene rearrangement The majority of the AMLR-positive-, but only two AMLR-negative TCCs showed cytolytic potential when assayed by a lectin-dependent assay. In addition, the proportion of clones able to produce interleukin 2 (IL-2) was higher among AMLR-positive- than AMLR-negative TCCs and the amount of IL-2 synthesized by AMLR-positive TCCs was significantly greater than that of AMLR-negative TCCs. In contrast, no difference in the profile of interleukin 4 (IL-4) and interferon-gamma (IFN-γ) production between AMLR-positive- and AMLR-negative TCCs was detected When AMLR-positive TCCs were analysed for their TCR beta and gamma gene rearrangements, a highly heterogeneous pattern was found. More importantly, TCCs derived from the same donor and displaying the same kind of TCR beta and gamma gene rearrangement showed different patterns of rearrangements, suggesting that CD4 + cells from LN involved by HD, displaying such an unusual functional profile, are polyclonal.
authors.affiliation: a Clinical Immunology Department, University of Florence, Florence.
authors.forename: E
authors.initials: E
authors.lastname: Maggi
authors.affiliation: a Clinical Immunology Department, University of Florence, Florence.
authors.forename: P
authors.initials: P
authors.lastname: Parronchi
authors.affiliation: a Clinical Immunology Department, University of Florence, Florence.
authors.forename: D
authors.initials: D
authors.lastname: Macchia
authors.affiliation: b Experimental Oncology Division, National Institute for Cancer Research and Therapy, Milan, Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Borrello
authors.affiliation: a Clinical Immunology Department, University of Florence, Florence.
authors.forename: P
authors.initials: P
authors.lastname: Biswas
authors.affiliation: b Experimental Oncology Division, National Institute for Cancer Research and Therapy, Milan, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Biassoni
authors.affiliation: a Clinical Immunology Department, University of Florence, Florence.
authors.forename: C
authors.initials: C
authors.lastname: Simonelli
authors.affiliation: c Haematology Departmen, University of Florence, Florence.
authors.forename: G
authors.initials: G
authors.lastname: Bellesi
authors.affiliation: d Othorynolaryngology Departments, University of Florence, Florence.
authors.forename: O
authors.initials: O
authors.lastname: Gallo
authors.affiliation: b Experimental Oncology Division, National Institute for Cancer Research and Therapy, Milan, Italy.
authors.lastname: Pierotti
authors.affiliation: a Clinical Immunology Department, University of Florence, Florence.
authors.forename: S
authors.initials: S
authors.lastname: Romagnani
keywords: Hodgkin's Disease; T cell clones; TCR beta and gamma gene rearrangements; autoreactive T cells