title: Lipid profile of platelets and platelet-derived microparticles in ovarian cancer.
abstract: BACKGROUND
  Ovarian cancer patients have a high risk of developing venous thrombosis. The membrane lipid bilayer of platelets and platelet-derived microparticles (PMP) provides a platform for assembly of coagulation proteins and generation of blood clots.
  
  
  METHODS
  We compared the lipid composition of platelets and PMPs in patients with ovarian cancer to those in healthy subjects. We used shotgun lipidomics to quantify 12 classes and 177 species of lipids.
  
  
  RESULTS
  We found a significant change in 2 classes of lipids in platelets and PMPs isolated from ovarian cancer patients: higher phosphatidylinositol and lower lyso-phosphatidylcholine. The level of 28 species of lipids was also significantly altered in the direction of an increase in the pro-coagulant and a reduction in the anticoagulant lipids. We found that cancer platelets expressed less lipid phosphate phosphatase 1 (LPP1), a key enzyme in phospholipid biosynthesis pathways, than normal platelets. The reduction in LPP1 might contribute to the changes in the lipid profile of cancer platelets.
  
  
  CONCLUSIONS
  Our results support a procoagulant lipid profile of platelets in ovarian cancer patients that can play a role in the increased risk of venous thrombosis in these patients.
  
  
  CONCLUSIONS
  As far as we are aware, our study is the first study on platelet lipidomics in ovarian cancer. The importance of our findings for the future studies are: 1) a similar change in lipid profile of platelets and PMP may be responsible for hypercoagulability in other cancers, and 2) plasma level of high-risk lipids for venous thrombosis may be useful biomarkers.
authors.affiliation: Department of Benign Hematology, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Qianghua
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute, 6400 Sanger Road, Orlando, FL 32827, USA.
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Benign Hematology, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Min Soon
authors.initials: MS
authors.lastname: Cho
authors.affiliation: Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute, 6400 Sanger Road, Orlando, FL 32827, USA.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Alpa M
authors.initials: AM
authors.lastname: Nick
authors.affiliation: Center for Translational Research on Inflammatory Diseases, Michael E. DeBakey Veterans Affairs Medical Center, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Perumal
authors.initials: P
authors.lastname: Thiagarajan
authors.affiliation: Department of Laboratory Medicine, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Fleur M
authors.initials: FM
authors.lastname: Aung
authors.affiliation: Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute, 6400 Sanger Road, Orlando, FL 32827, USA.
authors.forename: Xianlin
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Sood
authors.affiliation: Department of Benign Hematology, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Vahid
authors.initials: V
authors.lastname: Afshar-Kharghan
keywords: Hypercoagulability; Lipid phosphate phosphatase 1; Lipidomics; Ovarian cancer; Platelet; Venous thrombosis