title: Uniform Superparamagnetic Fe3O4/CMCS Composite Nanospheres for Lysozyme Adsorption.
abstract: In this study, uniform superparamagnetic Fe3O4/carboxymethyl chitosan composite nanospheres with high saturation magnetization were successfully synthesized via a modified inverse emulsion crosslinking approach, using genipin as a cross-linking agent. These nanospheres were then characterized, and their protein adsorption capacity was further investigated under various conditions. The implementation of a sonication treatment of a mixture containing Fe3O4 nanoparticles and carboxymethyl chitosan before the emulsion process significantly promoted the homogeneity of Fe3O4 nanoparticles in an aqueous phase system. The Fe3O4/carboxymethyl chitosan composite nanospheres were of uniform spherical structure, were approximately 230 nm in size, and possessed superparamagnetic characteristics with a mean saturation magnetization as high as 35 emu g(-1), corresponding to a magnetite content of 43%. Lysozyme was then employed as a model protein to investigate the effects of pH, incubation time and ion strength on the protein adsorption capacity of the as-synthesized composite nanospheres. The as-obtained composite nanospheres could serve as a promising candidate for fast and efficient protein adsorption.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.forename: Fang
authors.initials: F
authors.lastname: Lan
authors.forename: Ziyao
authors.initials: Z
authors.lastname: Liu
authors.forename: Shaohua
authors.initials: S
authors.lastname: Ma
authors.forename: Wenliao
authors.initials: W
authors.lastname: Li
authors.forename: Yao
authors.initials: Y
authors.lastname: Wu
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Gu
mesh_terms: D000327:Adsorption; D048271:Chitosan / Q000031:analogs & derivatives*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000737:chemistry*; D054118:Nanospheres; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D048271:Chitosan; D009113:Muramidase; D052203:Ferrosoferric Oxide