title: Association of multiparametric MRI quantitative imaging features with prostate cancer gene expression in MRI-targeted prostate biopsies.
abstract: Standard clinicopathological variables are inadequate for optimal management of prostate cancer patients. While genomic classifiers have improved patient risk classification, the multifocality and heterogeneity of prostate cancer can confound pre-treatment assessment. The objective was to investigate the association of multiparametric (mp)MRI quantitative features with prostate cancer risk gene expression profiles in mpMRI-guided biopsies tissues.Global gene expression profiles were generated from 17 mpMRI-directed diagnostic prostate biopsies using an Affimetrix platform. Spatially distinct imaging areas ('habitats') were identified on MRI/3D-Ultrasound fusion. Radiomic features were extracted from biopsy regions and normal appearing tissues. We correlated 49 radiomic features with three clinically available gene signatures associated with adverse outcome. The signatures contain genes that are over-expressed in aggressive prostate cancers and genes that are under-expressed in aggressive prostate cancers. There were significant correlations between these genes and quantitative imaging features, indicating the presence of prostate cancer prognostic signal in the radiomic features. Strong associations were also found between the radiomic features and significantly expressed genes. Gene ontology analysis identified specific radiomic features associated with immune/inflammatory response, metabolism, cell and biological adhesion. To our knowledge, this is the first study to correlate radiogenomic parameters with prostate cancer in men with MRI-guided biopsy.
authors.affiliation: Department of Radiation Oncology, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Radka
authors.initials: R
authors.lastname: Stoyanova
authors.affiliation: Department of Radiation Oncology, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Alan
authors.initials: A
authors.lastname: Pollack
authors.affiliation: Reserach and Development, GenomeDx Biosciences, Vancouver, BC, Canada.
authors.forename: Mandeep
authors.initials: M
authors.lastname: Takhar
authors.affiliation: Department of Urology, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Lynne
authors.affiliation: Department of Radiation Oncology, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Nestor
authors.initials: N
authors.lastname: Parra
authors.affiliation: Reserach and Development, GenomeDx Biosciences, Vancouver, BC, Canada.
authors.forename: Lucia L C
authors.initials: LL
authors.lastname: Lam
authors.affiliation: Reserach and Development, GenomeDx Biosciences, Vancouver, BC, Canada.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Alshalalfa
authors.affiliation: Reserach and Development, GenomeDx Biosciences, Vancouver, BC, Canada.
authors.forename: Christine
authors.initials: C
authors.lastname: Buerki
authors.affiliation: Department of Radiology, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Rosa
authors.initials: R
authors.lastname: Castillo
authors.affiliation: Department of Urology, University of Miami Miller School of Medicine, Miami, FL, USA.|Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Merce
authors.initials: M
authors.lastname: Jorda
authors.affiliation: Reserach and Development, GenomeDx Biosciences, Vancouver, BC, Canada.
authors.forename: Hussam Al-Deen
authors.initials: HA
authors.lastname: Ashab
authors.affiliation: Department of Urology, University of Miami Miller School of Medicine, Miami, FL, USA.|Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Oleksandr N
authors.initials: ON
authors.lastname: Kryvenko
authors.affiliation: Department of Urology, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Sanoj
authors.initials: S
authors.lastname: Punnen
authors.affiliation: Department of Urology, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Dipen J
authors.initials: DJ
authors.lastname: Parekh
authors.affiliation: Department of Radiation Oncology, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Abramowitz
authors.affiliation: Cancer Imaging and Metabolism, Moffitt Cancer Center, Tampa, FL, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Gillies
authors.affiliation: Reserach and Development, GenomeDx Biosciences, Vancouver, BC, Canada.
authors.forename: Elai
authors.initials: E
authors.lastname: Davicioni
authors.affiliation: Reserach and Development, GenomeDx Biosciences, Vancouver, BC, Canada.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Erho
authors.affiliation: Department of Radiation Oncology, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Adrian
authors.initials: A
authors.lastname: Ishkanian
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D063990:Gene Ontology; D006801:Humans; D061705:Image-Guided Biopsy / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging* / Q000235:genetics* / Q000473:pathology; D012189:Retrospective Studies
keywords: MRI-targeted biopsies; gene expression; multiparametric MRI; prostate cancer; radiogenomics