title: Coexistence of mcr-1 and blaNDM-1 in Escherichia coli from Venezuela.
abstract: We studied the presence of the mobile colistin resistance gene mcr-1 in human, animal, and environmental Enterobacteriaceae samples from Cumana, Venezuela, that were collected in 2015. The mcr-1 gene was detected in 2/93 Escherichia coli isolates from swine (novel ST452) and human (ST19) samples that were resistant to colistin. Whole-genome sequencing and transformation experiments identified mcr-1 on an IncI2 plasmid. One of the isolates also bore the widely spread carbapenemase NDM-1. A One Health approach is necessary to further elucidate the flux of these high-risk genes.
authors.affiliation: Department of Animal Health and Centro de Vigilancia Sanitaria Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Jose F
authors.initials: JF
authors.lastname: Delgado-Blas
authors.affiliation: Department of Animal Health and Centro de Vigilancia Sanitaria Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Cristina M
authors.initials: CM
authors.lastname: Ovejero
authors.affiliation: Department of Biomedicine, Instituto de Investigaciones en Biomedicina y Ciencias Aplicadas, Universidad de Oriente, Cumana, Venezuela.
authors.forename: Lorena
authors.initials: L
authors.lastname: Abadia-Patiño
authors.affiliation: Department of Animal Health and Centro de Vigilancia Sanitaria Veterinaria, Universidad Complutense de Madrid, Madrid, Spain bgzorn@ucm.es.
authors.forename: Bruno
authors.initials: B
authors.lastname: Gonzalez-Zorn
mesh_terms: D000818:Animals; D003091:Colistin / Q000494:pharmacology; D004285:Dogs; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D011200:Poultry; D013552:Swine; D014687:Venezuela; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; C000614037:MCR-1 protein, E coli; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1; D003091:Colistin