title: Total Cellular RNA Modulates Protein Activity.
abstract: RNA constitutes up to 20% of a cell's dry weight, corresponding to ∼20 mg/mL. This high concentration of RNA facilitates low-affinity protein-RNA quinary interactions, which may play an important role in facilitating and regulating biological processes. In the yeast Pichia pastoris, the level of ubiquitin-RNA colocalization increases when cells are grown in the presence of dextrose and methanol instead of methanol as the sole carbon source. Total RNA isolated from cells grown in methanol increases β-galactosidase activity relative to that seen with RNA isolated from cells grown in the presence of dextrose and methanol. Because the total cellular RNA content changes with growth medium, protein-RNA quinary interactions can alter in-cell protein biochemistry and may play an important role in cell adaptation, critical to many physiological and pathological states.
authors.affiliation: Department of Chemistry, State University of New York at Albany , 1400 Washington Avenue, Albany, New York 12222, United States.
authors.forename: Subhabrata
authors.initials: S
authors.lastname: Majumder
authors.affiliation: Department of Chemistry, State University of New York at Albany , 1400 Washington Avenue, Albany, New York 12222, United States.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: DeMott
authors.affiliation: Department of Chemistry, State University of New York at Albany , 1400 Washington Avenue, Albany, New York 12222, United States.
authors.forename: Sergey
authors.initials: S
authors.lastname: Reverdatto
authors.affiliation: Department of Chemistry, State University of New York at Albany , 1400 Washington Avenue, Albany, New York 12222, United States.
authors.forename: David S
authors.initials: DS
authors.lastname: Burz
authors.affiliation: Department of Chemistry, State University of New York at Albany , 1400 Washington Avenue, Albany, New York 12222, United States.
authors.forename: Alexander
authors.initials: A
authors.lastname: Shekhtman
mesh_terms: D010843:Pichia / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D012331:RNA, Fungal / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D012331:RNA, Fungal; D001616:beta-Galactosidase