title: Regeneration of mandibular defects using adipose tissue mesenchymal stromal cells in combination with human serum-derived scaffolds.
abstract: Bone regeneration is a challenging issue. Traditional solutions bring risks, potential complications, and morbidity. The aim of the present study was to regenerate critical-sized mandible defects in athymic rats with adipose tissue mesenchymal stromal cells (AT-MSCs) in combination with human serum-derived scaffolds. Two approaches to treatment were performed. The first approach used differentiated stromal cells that became osteogenic cell lines. The second approach used no pre-differentiation. Follow-up periods were 45 days and 90 days. Both cell types were combined with human serum-derived scaffolds. Afterward, histological (haematoxylin-eosin and Masson's Trichrome stain modified by Goldner), immunohistochemical (human vimentin and Stro-1), and radiological (microCT) studies were performed. The level of calcification between the groups was compared by analysis of variance, and statistical significance was set at p < 0.05. The results demonstrate that bone regeneration can be achieved with both undifferentiated and pre-differentiated cells, but that the structure and level of calcification were better achieved with pre-differentiated cells (p < 0.05). The scaffold is suitable for this cell type, is osteoconductive and simple to perform. This article highlights the possible application of adipose tissue mesenchymal stromal cells in combination with a non-mineralized scaffold in bone regeneration.
authors.affiliation: Oral and Maxillofacial Surgery Department (Head: Prof. J.C. de Vicente Rodríguez), Hospital Universitario Central de Asturias, Avda de Roma s/n, Oviedo, Asturias, 33011, Spain. Electronic address: napegomaxilo@gmail.com.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Peña González
authors.affiliation: Cell Therapy and Regenerative Medicine Unit, Hospital Universitario Central de Asturias, Avda de Roma s/n, Oviedo, Asturias, 33011, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Álvarez-Viejo
authors.affiliation: Bone and Mineral Research Unit, Hospital Universitario Central de Asturias, Avda de Roma s/n, Oviedo, Asturias, 33011, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Alonso-Montes
authors.affiliation: Cell Therapy and Regenerative Medicine Unit, Hospital Universitario Central de Asturias, Avda de Roma s/n, Oviedo, Asturias, 33011, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Menéndez-Menéndez
authors.affiliation: Private Practice, Clínica Calipso, Doctor Aquilino Hurlé 32 Bajo izda, 33203, Gijón, Asturias, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Gutiérrez Álvarez
authors.affiliation: Oral and Maxillofacial Surgery Department (Head: Prof. J.C. de Vicente Rodríguez), Hospital Universitario Central de Asturias, Avda de Roma s/n, Oviedo, Asturias, 33011, Spain.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: de Vicente Rodríguez
authors.affiliation: Cell Therapy and Regenerative Medicine Unit, Hospital Universitario Central de Asturias, Avda de Roma s/n, Oviedo, Asturias, 33011, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Otero Hernández
authors.affiliation: Community Center of Blood and Tissues of Asturias, CIBERER U714, c/Emilio Rodríguez Vigil, s/n, 33006, Oviedo, Asturias, Spain.
authors.forename: Álvaro
authors.initials: Á
authors.lastname: Meana Infiesta
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008334:Mandible / Q000601:surgery*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D011923:Rats, Nude; D054457:Tissue Scaffolds
keywords: AT-MSCs; Autologous; Bone; Regeneration; Scaffold; Serum