title: Mutation-based selection and analysis of Komagataeibacter hansenii HDM1-3 for improvement in bacterial cellulose production.
abstract: OBJECTIVE
  A low yield of bacterial cellulose (BC) always results from an excessive accumulation of organic acids. Screening and the selection of bacterial mutants with a low accumulation of organic acids is an efficient approach for improving BC production.
  
  
  RESULTS
  In combination with the proton suicide method (medium containing NaBr-NaBrO3), diethyl sulphate chemical mutagenesis coupled with60Co-γ irradiation treatment were performed for the screening and selection of desired mutant lines with a high yield of BC. Two high-yield strains, Br-3 and Co-5, as well as a low-yield strain, Br-12, were obtained. Amplified fragment length polymorphism (AFLP) was applied to explore the differences between the mutant lines and the wild type. For the Br-12 line, three specific fragments were verified, corresponding to TonB-dependent transport (TBDT), exopolysaccharides output protein (PePr) and an unknown gene. For Co-5, two specific fragments were matched, acsD and UDP-galactose-4-epimerase. In addition, metabolic analysis for the mutant lines indicated that BC production may be limited by excessive accumulation of organic acids in the fermentation. The limitation would be resolved by the cross-talk of genes involved in BC biosynthesis.
  
  
  CONCLUSIONS
  Reduced organic acid by-products from glucose in bypasses were found to be responsible for the high-yield BC synthesis in Komagataeibacter hansenii mutant strains.
  
  
  CONCLUSIONS
  The metabolic process was varied by mutagenesis-induced gene disruption of the metabolic products. A new idea was provided for the targeted screening and characterization of mutants in the future.
authors.affiliation: Laboratory of Microbiology and Laboratory of Crop Physiology, Chinese Academic Institute of Geography and Agroecology in Northeast, Changchun, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.
authors.forename: J
authors.initials: J
authors.lastname: Tian
authors.affiliation: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.
authors.forename: H
authors.initials: H
authors.lastname: Tian
authors.affiliation: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.affiliation: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.|Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Harbin, China.
authors.forename: W
authors.initials: W
authors.lastname: Ping
authors.affiliation: Laboratory of Microbiology and Laboratory of Crop Physiology, Chinese Academic Institute of Geography and Agroecology in Northeast, Changchun, China. tiancj@neigae.ac.cn.
authors.forename: C
authors.initials: C
authors.lastname: Tian
authors.affiliation: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China. leihong@hlju.edu.cn.|Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Harbin, China. leihong@hlju.edu.cn.
authors.forename: H
authors.initials: H
authors.lastname: Lei
mesh_terms: D016947:Acetobacteraceae / Q000235:genetics* / Q000378:metabolism*; D054458:Amplified Fragment Length Polymorphism Analysis; D002482:Cellulose / Q000096:biosynthesis*; D005285:Fermentation; D016296:Mutagenesis; D009154:Mutation
chemical_list: D002482:Cellulose
keywords: 
  Komagataeibacter hansenii
  ; amplified fragment length polymorphism; bacterial cellulose; metabolite; mutagenesis