title: Modulation of Dendritic-Epithelial Cell Responses against Sphingomonas Paucimobilis by Dietary Fibers.
abstract: Non-fermenting Gram-negative bacilli, such as Sphingomonas paucimobilis (S.paucimobilis), are among the most widespread causes of nosocomial infections. Up to now, no definitive guidelines exist for antimicrobial therapy for S. paucimobilis infections. As we have shown that some dietary fibers exhibit pronounced immune-regulatory properties, we hypothesized that specific immune active dietary fibers might modulate the responses against S. paucimobilis. We studied the immunomodulatory effects of dietary fibers against S. paucimobilis on cytokine release and maturation of human dendritic cells (DCs) in co-cultures of DCs and intestinal epithelial cells (IECs). S. paucimobilis infection resulted in increased release of pro-inflammatory cytokines and chemokines by DCs/IECs; these effects were strongly attenuated by specific dietary fibers. Chicory inulin, sugar beet pectin, and both starches had the strongest regulatory effects. IL-12 and TNF-α were drastically diminished upon exposure to chicory inulin and sugar beet pectin, or both starches. High-maize 260, was more effective in the reduction of chemokine release than the others fibers tested. In summary, chicory inulin, sugar beet pectin, High-maize 260, and Novelose 330 attenuate S. paucimobilis-induced cytokines. These results demonstrate that dietary fibers with a specific chemical composition can be used to manage immune responses against pathogens such as S. paucimobilis.
authors.affiliation: Top Institute Food and Nutrition, Wageningen, The Netherlands.|University of Groningen, University Medical Center Groningen, dept. Pathology and Medical Biology, Groningen, The Netherlands.
authors.forename: Miriam
authors.initials: M
authors.lastname: Bermudez-Brito
authors.affiliation: University of Groningen, University Medical Center Groningen, dept. Pathology and Medical Biology, Groningen, The Netherlands.
authors.forename: Marijke M
authors.initials: MM
authors.lastname: Faas
authors.affiliation: Top Institute Food and Nutrition, Wageningen, The Netherlands.|University of Groningen, University Medical Center Groningen, dept. Pathology and Medical Biology, Groningen, The Netherlands.
authors.forename: Paul
authors.initials: P
authors.lastname: de Vos
mesh_terms: D018920:Coculture Techniques; D003428:Cross Infection / Q000178:diet therapy* / Q000276:immunology / Q000382:microbiology; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D004043:Dietary Fiber / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D056747:Immunomodulation / Q000187:drug effects; D018664:Interleukin-12 / Q000235:genetics; D007444:Inulin / Q000737:chemistry / Q000494:pharmacology; D010368:Pectins / Q000737:chemistry / Q000494:pharmacology; D020578:Sphingomonas / Q000187:drug effects / Q000276:immunology / Q000472:pathogenicity; D013213:Starch / Q000737:chemistry / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D004043:Dietary Fiber; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D010368:Pectins; D013213:Starch; D007444:Inulin