title: Casticin inhibits lipopolysaccharide-induced acute lung injury in mice.
abstract: Casticin, an active compound isolated from Vitex Fructus, has been reported to have anti-inflammatory effects in previous studies. The present study was designed to evaluate the protective effects of casticin on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and clarify the underlying mechanisms. ALI was induced by intratracheal instillation of LPS. Casticin was administrated 1h before or after LPS challenge. The results showed that casticin decreased LPS-induced lung histopathological changes, MPO activity, and lung wet/dry weight ratio. Casticin also inhibited the numbers of inflammatory cells and the levels of inflammatory cytokines TNF-α, IL-6, and IL-1β production. Furthermore, casticin suppressed LPS-induced NF-κB activation, and NLRP3, ASC, and caspase-1 expression. In conclusion, the results suggested that casticin had a protective effect against LPS-induced ALI. Casticin protected against LPS-induced ALI by inhibiting inflammatory cytokines production through the inhibition of NF-κB and NLRP3 signaling pathways.
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Wang
authors.affiliation: Harbin Children Hospital, Harbin 150010, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Jiakun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Electronic address: wangwenbolb@sina.com.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Wang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D053468:CARD Signaling Adaptor Proteins; D020170:Caspase 1 / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D005786:Gene Expression Regulation / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D051017:Apoptosis Regulatory Proteins; D053468:CARD Signaling Adaptor Proteins; D005419:Flavonoids; D008070:Lipopolysaccharides; D016328:NF-kappa B; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C498534:Pycard protein, mouse; C054133:casticin; D009195:Peroxidase; D020170:Caspase 1
keywords: Acute lung injury; Casticin; LPS; NF-κB; NLRP3