title: Lentivirus mediated RNA interference of EMMPRIN (CD147) gene inhibits the proliferation, matrigel invasion and tumor formation of breast cancer cells.
abstract: BACKGROUND
  Overexpression of extracellular matrix metalloproteinase inducer (EMMPRIN) or cluster of differentiation 147 (CD147), a glycoprotein enriched on the plasma membrane of tumor cells, promotes proliferation, invasion, metastasis, and survival of malignant tumor cells. In this study, we sought to examine the expression of EMMPRIN in breast tumors, and to identify the potential roles of EMMPRIN on breast cancer cells.
  
  
  METHODS
  EMMPRIN expression in breast cancer tissues was assessed by immunohistochemistry. We used a lentivirus vector-based RNA interference (RNAi) approach expressing short hairpin RNA (shRNA) to knockdown EMMPRIN gene in breast cancer cell lines MDA-MB-231 and MCF-7. In vitro, Cell proliferative, invasive potential were determined by Cell Counting Kit (CCK-8), cell cycle analysis and matrigel invasion assay, respectively. In vivo, tumorigenicity was monitored by inoculating tumor cells into breast fat pad of female nude mice.
  
  
  RESULTS
  EMMPRIN was over-expressed in breast tumors and breast cancer cell lines. Down-regulation of EMMPRIN by lentivirus vector-based RNAi led to decreased cell proliferative, decreased matrigel invasion in vitro, and attenuated tumor formation in vivo.
  
  
  CONCLUSIONS
  High expression of EMMPRIN plays a crucial role in breast cancer cell proliferation, matrigel invasion and tumor formation.
authors.affiliation: Department of Thyroid and Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Breast Surgery, the Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Thyroid and Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Thyroid and Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Laboratory of Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Wentong
authors.initials: W
authors.lastname: Meng
authors.affiliation: Department of Thyroid and Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D051926:Basigin / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; D051926:Basigin; D020780:Matrix Metalloproteinase 9
keywords: EMMPRIN; MMP-9; RNA interference (RNAi); breast cancer; lentivirus; short hairpin RNA (shRNA)