title: miR-338-3p suppresses epithelial-mesenchymal transition and metastasis in human nonsmall cell lung cancer.
abstract: OBJECTIVE
  MicroRNAs are important modulators of the cellular epithelial-mesenchymal transition (EMT) process and are associated with metastasis in human nonsmall cell lung cancer (NSCLC). In this study, we tried to investigate the role of miR-338-3p in NSCLC cells.
  
  
  METHODS
  Real-time polymerase chain reaction was applied to quantify the expression levels of miR-338-3p, as well as EMT-associated molecules in NSCLC cells. Wound healing and transwell assays were performed to evaluate the migration and invasion capacities, respectively. Dual-luciferase reporter assay was finally performed to determine the targeting of zinc finger E-box-binding protein 2 (ZEB2) by miR-338-3p.
  
  
  RESULTS
  We found that miR-338-3p was significantly reduced in NSCLC cell lines. Forced expression of miR-338-3p in A549 cells led to the suppression of migration/invasion capacity and inhibition of epithelial markers. In addition, we proved that miR-338-3p could directly target ZEB2.
  
  
  CONCLUSIONS
  In general, we summarized that miR-338-3p could inhibit EMT and metastasis of human NSCLC cells, which probably via directly targeting ZEB2 expression.
authors.affiliation: Department of Respiratory, The Sixth Affiliated Hospital of Wenzhou Medical University, People's Hospital of Lishui City, Zhejiang, China.
authors.forename: W
authors.initials: W
authors.lastname: Hong-Yuan
authors.affiliation: Department of Respiratory, The Sixth Affiliated Hospital of Wenzhou Medical University, People's Hospital of Lishui City, Zhejiang, China.
authors.forename: C
authors.initials: C
authors.lastname: Xiao-Ping
mesh_terms: D000072283:A549 Cells; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics; D000074165:Zinc Finger E-box Binding Homeobox 2
chemical_list: D018398:Homeodomain Proteins; C532050:MIRN338 microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins; C492363:ZEB2 protein, human; D000074165:Zinc Finger E-box Binding Homeobox 2