title: Impaired Cytotoxic Activity of Interleukin 2 (IL 2)-cultured Large Granular Lymphocytes (LGL) in Childhood Acute Lymphoblastic Leukemia. Quantitative Measurement of IL 2 Receptor Expression of IL 2-cultured LGL.
abstract: Large granular lymphocytes (LGL) of the natural killer (NK) cell lineage were highly purified and their interleukin 2 (IL 2) receptors (IL 2R) were investigated in childhood acute lymphoblastic leukemia (ALL). Not only NK activity but also 3-day recombinant IL 2-cultured lymphokine activated killer (LAK) activity were decreased in ALL, despite normal percentages of LGL, CD16 (Leul1)(+) and CD56 (NKH1)(+) cells. The cytotoxic activity of IL 2-cultured LGL, but not IL 2-cultured T cells, was significantly decreased in ALL, indicating a selective defect of LGL among the killer cells. IL 2R (CD25) numbers/cell of 3-day IL 2-cultured LGL were not decreased in ALL by flow cytometric analysis. Scatchard plot analysis demonstrated that high affinity IL 2R numbers/cell of 3-day IL 2-cultured LGL were normal but the binding affinity level of the receptors in these cells in ALL was one-third of that in the similarly-cultured control cells, suggesting inadequate IL 2-IL 2R interaction is responsible, at least in part, for their reduced cytotoxic activity.
authors.affiliation: a Department of Pediatrics, Shinshu University School of Medicine, 3-1-1, Asahi, Matsumoto, 390, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yamada
authors.affiliation: a Department of Pediatrics, Shinshu University School of Medicine, 3-1-1, Asahi, Matsumoto, 390, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ichikawa
authors.affiliation: a Department of Pediatrics, Shinshu University School of Medicine, 3-1-1, Asahi, Matsumoto, 390, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Komiyama
keywords: Large granular lymphocytes; childhood acute lymphoblastic leukemia; interleukin 2; interleukin 2 receptor