title: Dynamics of glucosinolate-myrosinase system during Plutella xylostella interaction to a novel host Lepidium latifolium L.
abstract: Plutella xylostella L. is a notorious pest of cruciferous crops causing worldwide losses of $4-5 billion per year. Developing classical biological control to this pest include an introduction of host plants that act as natural enemies showing deviation from the preference-performance regimen in the evolutionary ecology of plant-insect interactions. The present study was designed to understand the role of glucosinolate-myrosinase system during P. xylostella interactions with a novel host. Adult moth preference and larval performance study were conducted on a novel host Lepidium latifolium L. (LL) that has high sinigrin content and was compared with its laboratory host Arabidopsis thaliana (AT). The glucosinolate-myrosinase system was studied in a time course experiment during larval feeding in choice and no-choice experiments. Adult moths visit and prefers LL over AT for oviposition. Conversely, LL leaves were not preferred and proved detrimental for P. xylostella larvae. Aliphatic and indolic glucosinolates were found to decrease significantly (p≤0.05) in AT during initial 12h of P. xylostella challenge, whereas, they were not affected in LL. Also, MYB transcription factor expression and myrosinase activity in LL do not suggest a typical host response to a specialist insect. This preference-performance mismatch of P. xylostella on LL mediated by glucosinolate pattern suggests that this novel plant could be utilized in P. xylostella management.
authors.affiliation: Biodiversity and Applied Botany Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu, J & K 180001, India; Academy of Scientific and Innovative Research, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu, J & K 180001 India.
authors.forename: Tarandeep
authors.initials: T
authors.lastname: Kaur
authors.affiliation: Biodiversity and Applied Botany Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu, J & K 180001, India; Academy of Scientific and Innovative Research, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu, J & K 180001 India.
authors.forename: Rohini
authors.initials: R
authors.lastname: Bhat
authors.affiliation: Biodiversity and Applied Botany Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu, J & K 180001, India.
authors.forename: Manu
authors.initials: M
authors.lastname: Khajuria
authors.affiliation: Formulation and Drug Development Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu, J & K 180001, India.
authors.forename: Ruchika
authors.initials: R
authors.lastname: Vyas
authors.affiliation: Entomology Laboratory, Institute of Himalayan Bioresource Technology (CSIR), Post Box No. 6, Palampur, HP 176061, India.
authors.forename: Anika
authors.initials: A
authors.lastname: Kumari
authors.affiliation: Entomology Laboratory, Institute of Himalayan Bioresource Technology (CSIR), Post Box No. 6, Palampur, HP 176061, India.
authors.forename: Gireesh
authors.initials: G
authors.lastname: Nadda
authors.affiliation: Natural Product Chemistry Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu, J & K 180001, India.
authors.forename: Ram
authors.initials: R
authors.lastname: Vishwakarma
authors.affiliation: Biodiversity and Applied Botany Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu, J & K 180001, India; Academy of Scientific and Innovative Research, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu, J & K 180001 India. Electronic address: dhirajvyas@rediffmail.com.
authors.forename: Dhiraj
authors.initials: D
authors.lastname: Vyas
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000737:chemistry* / Q000201:enzymology; D005518:Food Preferences; D005961:Glucosinolates / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007814:Larva / Q000254:growth & development / Q000502:physiology; D029686:Lepidium / Q000737:chemistry* / Q000201:enzymology; D009036:Moths / Q000254:growth & development / Q000502:physiology*; D010058:Oviposition; D010940:Plant Proteins / Q000378:metabolism*
chemical_list: D005961:Glucosinolates; D010940:Plant Proteins; C010330:sinigrin; D006026:Glycoside Hydrolases; C022739:thioglucosidase
keywords: Larval feeding; Lepidium latifolium; MYB transcription factor; Myrosinase; Oviposition; Preference-performance; Sinigrin