title: Isolate and Sub-fractionate Cell Membranes fromCaulobacter crescentus.
abstract: Cell membranes fromCaulobactercan be isolated and separated into inner and outer membranes according their characteristic buoyant densities on a sucrose gradient. Fractionation can be used to determine the localisation of uncharacterised proteins and to enrich protein complexes present in either of these membranes for biochemical analysis such blue-native PAGE and immunoprecipitation.
authors.affiliation: Biochemistry and Molecular Biology Department, Monash University, Melbourne, Australia.
authors.forename: Khatira
authors.initials: K
authors.lastname: Anwari