title: An Expedient Synthesis of Fluorescent Labeled Ceramide-1-phosphate Analogues.
abstract: A synthesis for fluorescent analogs of ceramide-1-phosphate bearing 9-anthrylvinyl or 4,4-difluoro-3a,4a-diaza-s-indacene-8-yl (Me4-BODIPY) fluorophore at co-position of fatty acid residue was carried out. The key stage of the synthesis is hydrolysis of corresponding sphingomyelins catalyzed by phospholipase D fromStreptomyces chromofuscus; the enzymatic yield has been raised to 50-70% by appliance of organic solvent in the incubation medium.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997 Russian.
authors.forename: I A
authors.initials: IA
authors.lastname: Boldyrev
authors.affiliation: Hormel Institute, University of Minnesota, Austin, MN 55912 USA.
authors.forename: R E
authors.initials: RE
authors.lastname: Brown
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997 Russian.
authors.forename: J G
authors.initials: JG
authors.lastname: Molotkovsky
keywords: BODIPY; anthrylvinyl; ceramide-1-phosphate; fluorescent lipid probes; phospholipase D; synthesis