title: EZH2 is overexpressed in laryngeal squamous cell carcinoma and enhances the stem-like properties of AMC-HN-8 cells.
abstract: The enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) histone methyltransferase is the catalytic subunit of polycomb repressive complex 2 (PRC2), which is important for epigenetic regulation. EZH2 is highly expressed in various types of tumors, and its high-level expression promotes the progression and invasion of certain tumors. However, the expression level of EZH2 and its functions in laryngeal squamous cell carcinomas are unknown. In the present study, the level of EZH2 expression in laryngeal squamous cell carcinomas was evaluated using immunochemical staining and reverse transcription-quantitative polymerase chain reaction. EZH2 was overexpressed in AMC-HN-8 cells with lentiviral transfection. Cell proliferation, apoptosis, cell-cycle, chemotherapy-sensitivity andin vivotumorigenic assays were performed. The results indicated that EZH2 was highly expressed in laryngeal squamous cell carcinomas. Additionally, EZH2 overexpression promoted proliferation, accelerated cell-cycle progression and enhanced the tumorigenicity in laryngeal squamous cancer cells. More importantly, EZH2 enhanced the chemotherapy resistance of these cells. Overall, the results indicated that EZH2 promotes the progression of laryngeal squamous cell cancer and could be a potential chemotherapeutic target for the treatment of such cancer.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Affiliated Eye, Ear, Nose and Throat Hospital of Fudan University, Shanghai 200031, P.R. China.
authors.forename: Jiameng
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Affiliated Eye, Ear, Nose and Throat Hospital of Fudan University, Shanghai 200031, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Affiliated Eye, Ear, Nose and Throat Hospital of Fudan University, Shanghai 200031, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Affiliated Eye, Ear, Nose and Throat Hospital of Fudan University, Shanghai 200031, P.R. China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Affiliated Eye, Ear, Nose and Throat Hospital of Fudan University, Shanghai 200031, P.R. China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Duo
authors.affiliation: Research Center, Affiliated Eye, Ear, Nose and Throat Hospital of Fudan University, Shanghai 200031, P.R. China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
keywords: AMC-HN-8 cell line; enhancer of zeste 2 polycomb repressive complex 2 subunit; expression; laryngeal squamous cell carcinoma; stem-like properties