title: Antibiotic resistance genes fate and removal by a technological treatment solution for water reuse in agriculture.
abstract: In order to mitigate the potential effects on the human health which are associated to the use of treated wastewater in agriculture, antibiotic resistance genes (ARGs) are required to be carefully monitored in wastewater reuse processes and their spread should be prevented by the development of efficient treatment technologies. Objective of this study was the assessment of ARGs reduction efficiencies of a novel technological treatment solution for agricultural reuse of municipal wastewaters. The proposed solution comprises an advanced biological treatment (Sequencing Batch Biofilter Granular Reactor, SBBGR), analysed both al laboratory and pilot scale, followed by sand filtration and two different disinfection final stages: ultraviolet light (UV) radiation and peracetic acid (PAA) treatments. By Polymerase Chain Reaction (PCR), the presence of 9 ARGs (ampC, mecA, ermB, sul1, sul2, tetA, tetO, tetW, vanA) were analysed and by quantitative PCR (qPCR) their removal was determined. The obtained results were compared to the reduction of total bacteria (16S rDNA gene) and of a faecal contamination indicator (Escherichia coli uidA gene). Only four of the analysed genes (ermB, sul1, sul2, tetA) were detected in raw wastewater and their abundance was estimated to be 3.4±0.7 x10(4) - 9.6±0.5 x10(9) and 1.0±0.3 x10(3) to 3.0±0.1 x10(7) gene copies/mL in raw and treated wastewaters, respectively. The results show that SBBGR technology is promising for the reduction of ARGs, achieving stable removal performance ranging from 1.0±0.4 to 2.8±0.7 log units, which is comparable to or higher than that reported for conventional activated sludge treatments. No reduction of the ARGs amount normalized to the total bacteria content (16S rDNA), was instead obtained, indicating that these genes are removed together with total bacteria and not specifically eliminated. Enhanced ARGs removal was obtained by sand filtration, while no reduction was achieved by both UV and PAA disinfection treatments tested in our study.
authors.affiliation: Water Research Institute, CNR, Via Salaria Km 29.600, 00015 Monterotondo, RM, Italy.
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Luprano
authors.affiliation: Water Research Institute, CNR, Via F. De Blasio 5, 70123 Bari, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: De Sanctis
authors.affiliation: Water Research Institute, CNR, Via F. De Blasio 5, 70123 Bari, Italy.
authors.forename: Guido
authors.initials: G
authors.lastname: Del Moro
authors.affiliation: Water Research Institute, CNR, Via F. De Blasio 5, 70123 Bari, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Di Iaconi
authors.affiliation: Water Research Institute, CNR, Via F. De Blasio 5, 70123 Bari, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Lopez
authors.affiliation: Water Research Institute, CNR, Via Salaria Km 29.600, 00015 Monterotondo, RM, Italy. Electronic address: levantesi@irsa.cnr.it.
authors.forename: Caterina
authors.initials: C
authors.lastname: Levantesi
mesh_terms: D000383:Agriculture / Q000379:methods*; D001419:Bacteria / Q000235:genetics*; D000074067:Conservation of Water Resources / Q000379:methods*; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D014865:Waste Disposal, Fluid / Q000295:instrumentation / Q000379:methods*; D062065:Wastewater / Q000382:microbiology
chemical_list: D062065:Wastewater
keywords: Antibiotic resistance genes; Disinfection; E.coli; Sequencing Batch Biofilter Granular Reactor; Wastewater treatment; Water reuse