title: 5-Hydroxymethylcytosine (5-hmC) Specific Enrichment.
abstract: 5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA modification in mammalian genomes. This protocol is to be a highly efficient and selective chemical approach to label and capture 5-hmC, taking advantage of a bacteriophage enzyme that adds a glucose moiety to 5-hmC specifically, which could in turn be used for high-throughput mapping via next-generation sequencing.
authors.affiliation: Department of Human Genetics, Emory University School of Medicine, tlanta, USA.
authors.forename: Keith E
authors.initials: KE
authors.lastname: Szulwach
authors.affiliation: Department of Chemistry and Institute for Biophysical Dynamics, University of Chicago, Chicago, USA.
authors.forename: Chun-Xiao
authors.initials: CX
authors.lastname: Song
authors.affiliation: Department of Chemistry and Institute for Biophysical Dynamics, University of Chicago, Chicago, USA.
authors.forename: Chuan
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Chemistry and Institute for Biophysical Dynamics, University of Chicago, Chicago, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Jin