title: MiR-375 and miR-200c as predictive biomarkers of islet isolation and transplantation in total pancreatectomy with islet autotransplantation.
abstract: BACKGROUND
  Total pancreatectomy with islet autotransplantation (TPIAT) is a promising treatment for refractory chronic pancreatitis. Predictable biomarkers for the endocrine function after transplantation would be helpful in selecting patients for TPIAT. This study aims to identify novel biomarkers for predicting the outcome of islet isolation and transplantation in TPIAT patients.
  
  
  METHODS
  This paper studied microRNA of 31 TPIAT patients and 11 deceased donors from plasma samples before TPIAT. MiR-7, miR-200a, miR-200c, miR-320, and miR-375 were analyzed along with patient characteristics and the outcomes of islet isolation and transplantation via univariate and multivariate regression analysis.
  
  
  RESULTS
  MiR-375 before TPIAT showed a significant correlation with ∆C-peptide (r = -0.396, P = 0.03) and post-digestion islet count (r = -0.372, P = 0.04). And also miR-200c was significantly correlated with insulin requirement, C-peptide, and SUITO index at 1 year after transplantation. Moreover it was confirmed that miR-200c was a predictable factor of endocrine outcome in multi regression analysis (coefficient = -7.081, P = 0.001).
  
  
  CONCLUSIONS
  We concluded that miR-375 and miR-200c could potentially serve as novel biomarkers in predicting the islet yield in islet isolation and the metabolic function after transplantation for chronic pancreatitis patients.
authors.affiliation: Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas, USA.
authors.forename: Gumpei
authors.initials: G
authors.lastname: Yoshimatsu
authors.affiliation: Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas, USA.
authors.forename: Morihito
authors.initials: M
authors.lastname: Takita
authors.affiliation: Transplant Division, Department of Surgery, Virginia Commonwealth University Medical Center, Richmond, Virginia, USA.
authors.forename: Mazhar A
authors.initials: MA
authors.lastname: Kanak
authors.affiliation: Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas, USA.
authors.forename: Waqas Z
authors.initials: WZ
authors.lastname: Haque
authors.affiliation: Institute of Biomedical Studies, Baylor University, Waco, Texas, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Chang
authors.affiliation: Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas, USA.
authors.forename: Prathab Balaji
authors.initials: PB
authors.lastname: Saravanan
authors.affiliation: Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas, USA.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Lawrence
authors.affiliation: Transplant Division, Department of Surgery, Virginia Commonwealth University Medical Center, Richmond, Virginia, USA.
authors.forename: Marlon F
authors.initials: MF
authors.lastname: Levy
authors.affiliation: Annette C. and Harold C. Simmons Transplant Institute, Baylor University Medical Center, Dallas, Texas, USA. bashoo.naziruddin@bswhealth.org.|Islet Cell Laboratory, Baylor Simmons Transplant Institute, 3410 Worth Street, Suite 950, Dallas, TX, 75246, USA. bashoo.naziruddin@bswhealth.org.
authors.forename: Bashoo
authors.initials: B
authors.lastname: Naziruddin
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D015331:Cohort Studies; D005260:Female; D006084:Graft Rejection; D006085:Graft Survival; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D016015:Logistic Models; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000378:metabolism*; D008875:Middle Aged; D015999:Multivariate Analysis; D010180:Pancreatectomy / Q000379:methods*; D050500:Pancreatitis, Chronic / Q000175:diagnosis / Q000601:surgery*; D011237:Predictive Value of Tests; D020127:Recovery of Function; D012189:Retrospective Studies; D018709:Statistics, Nonparametric; D014182:Transplantation, Autologous; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D007328:Insulin; D035683:MicroRNAs
keywords: Biomarker; Islet; MicroRNA; Pancreas; Transplantation