title: Boswellic acid disables signal transduction of IL-6-STAT-3 in Ehrlich ascites tumor bearing irradiated mice.
abstract: Boswellic acid (BA) is known for its ability to trigger apoptosis as well as to inhibit angiogenesis in tumor tissue. In this study, we investigated the effect of BA on the IL-6-STAT-3 signalling pathway in irradiated mice bearing solid tumors of Ehrlich ascites carcinoma (EAC). For this, we administered BA (25 mg·(kg body mass)(-1)·day(-1), by intraperitoneal injection) to mice with EAC, and then exposed them to 4 Gy of gamma radiation. Data analyses of the results revealed a specific impact from BA on IL-6R mRNA and survivin mRNA in EACs and irradiated EAC-bearing mice. Also, significant improvements were observed in the protein expression of JAK-1, P-JAK-1, STAT-3, P-STAT-3, and caspase-3, as well as VEGF and IL-6 levels. We propose that BA interfered with IL-6-STAT-3 signal transduction, thereby preventing the activation of caspase-3 and subsequently triggering the process of apoptosis. However, the alternative angiogenesis pathway, which includes the over-expression of VEGF and which depends on IL-6-STAT-3 signalling, was inhibited by the action of BA. Thus, we recommend that therapeutic strategies for cancer should include treatment with BA.
authors.affiliation: Radiation Biology Department National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.|Radiation Biology Department National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
authors.forename: Enas Mahmoud
authors.initials: EM
authors.lastname: Moustafa
authors.affiliation: Radiation Biology Department National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.|Radiation Biology Department National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
authors.forename: Noura Magdy
authors.initials: NM
authors.lastname: Thabet
authors.affiliation: Radiation Biology Department National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.|Radiation Biology Department National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
authors.forename: Khaled Shaaban
authors.initials: KS
authors.lastname: Azab
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D015153:Blotting, Western; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000528:radiation effects; D007124:Immunoenzyme Techniques; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015850:Interleukin-6; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D014315:Triterpenes; C054625:boswellic acid
keywords: IL-6R; P-JAK-1; P-STAT-3; STAT-3; acide boswellique; boswellic acid; gamma radiation; irradiation gamma