title: Preparation of Polypyrrole-Protein Composite Films and the Electrochemically Controlled Release of Proteins.
abstract: It is fabricated that an electrically controlled release system based on the (poly-L-lactic acid)-mixed polypyrrole (PLLA-PPy) films through casting film of PPy and PLLA mixed solution on the glass plate, in which polyglutamic acid (PGlu)-doped PPy nanoparticles (NPs) with -50 nm diameter are synthesized via chemical oxidation. Surface conductivity of the composite film is (3.33 ± 2.01) x 10(-3) S/cm. Bovine serum albumin (BSA), as a model protein drug, is chemically linked onto the composite film via carbodiimide chemistry due to the good surface nano-structure of PLLA-PPy film. The releases of BSA from PLLA-PPy film under constant current and constant voltage can be achieved using the two-electrode electrochemical system. 6 h accumulative releases of BSA are 276 μg/cm2 and 176 μg/cm2 under 3 mA and 1 V electrical stimulation, respectively, accompanied with de-doping of PGlu and separation of a part of PPy NPs from the composite film. The results of cell experiment indicate that PGlu-doped PPy NPs in the prepared composite film have good cyto-compatibility. These results suggest that PPy-PLLA composite film would be able to be applied in the construction of degradable protein-drug-loaded scaffold for nerve tissue repair.
authors.forename: Juan
authors.initials: J
authors.lastname: Jin
authors.forename: Zhongbing
authors.initials: Z
authors.lastname: Huang
authors.forename: Guangfu
authors.initials: G
authors.lastname: Yin
authors.forename: Jiangli
authors.initials: J
authors.lastname: Lin
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Li
authors.forename: Donghui
authors.initials: D
authors.lastname: Han
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002460:Cell Line; D055664:Electrochemical Techniques; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D011506:Proteins; D011758:Pyrroles; C067635:polypyrrole