title: Kynurenic Acid Induces Impairment of Oligodendrocyte Viability: On the Role of Glutamatergic Mechanisms.
abstract: Kynurenic acid (KYNA) is an end stage product of tryptophan metabolism with a variety of functions in the human body, both in the central nervous system (CNS) and in other organs. Although its activity in the human brain has been widely studied and effects on neural cells were emphasized, the effect of KYNA on oligodendroglial cells remains unknown. Present study aims at describing the activity of high concentration of KYNA in OLN-93 cells. The inhibition of OLN-93 oligodendrocytes viability by KYNA in a medium with reduced serum concentration has been demonstrated. Although decreased metabolic activity of KYNA treated OLN-93 cells was shown, the cells proliferation was not altered. KYNA treatment did not alter morphology as well as expression level of cell cycle and proliferation regulating proteins. Furthermore, glutamate receptor antagonists and agonists did not alter the inhibitory effect of KYNA on viability of OLN-93 oligodendrocytes. This study contributes to the elucidation of effects of KYNA on oligodendrocytes in vitro, yet further analyses are necessary to explain the mechanisms behind the damage and loss of myelin sheaths.
authors.affiliation: Department of Medical Biology, Institute of Rural Health, Lublin, Poland.|Department of Pharmacology, Medical University, Lublin, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Langner
authors.affiliation: Department of Medical Biology, Institute of Rural Health, Lublin, Poland.
authors.forename: Marta K
authors.initials: MK
authors.lastname: Lemieszek
authors.affiliation: Department of Pathology and Molecular Medicine, M. deGroote School of Medicine, McMaster University, Hamilton, Canada.|Department of Clinical Pathomorphology, Medical University, Lublin, Poland.
authors.forename: Jacek M
authors.initials: JM
authors.lastname: Kwiecień
authors.affiliation: Department of Pharmacology, Medical University, Lublin, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Rajtar
authors.affiliation: Department of Medical Biology, Institute of Rural Health, Lublin, Poland.|Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Rzeski
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University, Lublin, Poland. turskiwa@op.pl.
authors.forename: Waldemar A
authors.initials: WA
authors.lastname: Turski
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005971:Glutamates / Q000378:metabolism*; D007736:Kynurenic Acid / Q000378:metabolism* / Q000494:pharmacology; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
chemical_list: D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates; D007736:Kynurenic Acid
keywords: Cell viability; Glutamate receptors; Kynurenic acid (KYNA); Myelin; OLN-93; Oligodendrocytes