title: Extracellular synthesis of cuprous selenide nanospheres by a biological-chemical coupling reduction process in an anaerobic microbial system.
abstract: Biosynthesis of metal nanoparticles represents a clean, eco-friendly and sustainable "green chemistry" engineering. Lately, a number of metal selenides were successfully synthesized by biological methods. Here, cuprous selenide (Cu2Se) nanospheres were prepared under mild conditions by a novel biological-chemical coupling reduction process. The simple process takes place between EDTA-Cu and Na2SeO3in presence of an alkaline solution containing NaBH4and a selenite-reducing bacteria, Pantoea agglomerans. It is noteworthy that the isolated Pantoea agglomerans and Cu+ions, where the latter are obtained from reducing Cu2+ions by NaBH4, play a key role, and Cu+ions not only can promote the generation of Se2-ions as a catalyst, but also can react with Se2-ions to form Cu2Se. XRD pattern, SEM, and TEM images indicated that Cu2Se nanoparticles were tetragonal crystal structure and the nanospheres diameter were about 100 nm. EDX, UV-vis, and FTIR spectra show that the biosynthesized Cu2Se nanospheres are wrapped by protein and have a better stability. This work first proposes a new biosynthesis mechanism, and has important reference value for biological preparation of metal selenide nanomaterials. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1264-1270, 2016.
authors.affiliation: Department of Energy & Environment Material, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yue
authors.affiliation: Department of Energy & Environment Material, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, P.R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Energy & Environment Material, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, P.R. China.
authors.forename: Shiyue
authors.initials: S
authors.lastname: Qi
authors.affiliation: Department of Energy & Environment Material, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, P.R. China. xinbaoping@bit.edu.cn.
authors.forename: Baoping
authors.initials: B
authors.lastname: Xin
mesh_terms: D003300:Copper / Q000378:metabolism*; D054118:Nanospheres / Q000378:metabolism*; D020636:Pantoea / Q000302:isolation & purification / Q000378:metabolism*; D013440:Sulfides / Q000378:metabolism*
chemical_list: D013440:Sulfides; C099343:cuprous sulfide; D003300:Copper
keywords: Cu2Se; Pantoea agglomerans; biological-chemical coupling reduction; biosynthesis; nanospheres