title: Sequential Optimization Methods for Augmentation of Marine Enzymes Production in Solid-State Fermentation: l-Glutaminase Production a Case Study.
abstract: There is an increased l-glutaminase market worldwide due to its relevant industrial applications. Salt tolerance l-glutaminases play a vital role in the increase of flavor of different types of foods like soya sauce and tofu. This chapter is presenting the economically viable l-glutaminases production in solid-state fermentation (SSF) by Aspergillus flavus MTCC 9972 as a case study. The enzyme production was improved following a three step optimization process. Initially mixture design (MD) (augmented simplex lattice design) was employed to optimize the solid substrate mixture. Such solid substrate mixture consisted of 59:41 of wheat bran and Bengal gram husk has given higher amounts of l-glutaminase. Glucose and l-glutamine were screened as a finest additional carbon and nitrogen sources for l-glutaminase production with help of Plackett-Burman Design (PBD). l-Glutamine also acting as a nitrogen source as well as inducer for secretion of l-glutaminase from A. flavus MTCC 9972. In the final step of optimization various environmental and nutritive parameters such as pH, temperature, moisture content, inoculum concentration, glucose, and l-glutamine levels were optimized through the use of hybrid feed forward neural networks (FFNNs) and genetic algorithm (GA). Through sequential optimization methods MD-PBD-FFNN-GA, the l-glutaminase production in SSF could be improved by 2.7-fold (453-1690U/g).
authors.affiliation: Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India. Electronic address: sathish@niot.res.in.
authors.forename: T
authors.initials: T
authors.lastname: Sathish
authors.affiliation: Bioprospecting Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur, India.
authors.forename: K B
authors.initials: KB
authors.lastname: Uppuluri
authors.affiliation: Krishna University, Machilipatnam, India.
authors.forename: P
authors.initials: P
authors.lastname: Veera Bramha Chari
authors.affiliation: Center for Biotechnology, Andhra University, Visakhapatnam, India.
authors.forename: D
authors.initials: D
authors.lastname: Kezia
mesh_terms: D059001:Aquatic Organisms / Q000201:enzymology* / Q000382:microbiology; D001231:Aspergillus flavus / Q000201:enzymology / Q000254:growth & development; D003365:Costs and Cost Analysis; D005285:Fermentation; D005972:Glutaminase / Q000096:biosynthesis* / Q000191:economics
chemical_list: D005972:Glutaminase
keywords: Genetic algorithms; Mixture design; Neural networks; Optimization; Plackett–Burman design; Solis-state fermentation; l-Glutaminase