title: IL-33 Contributes to Schistosoma japonicum-induced Hepatic Pathology through Induction of M2 Macrophages.
abstract: Interleukin (IL)-33 is involved in T helper (Th)2-biased immune responses in mice infected with Schistosoma, but the precise mechanism remains to be elucidated. Herein, we investigated the role of IL-33 and its receptor ST2L in hepatic granuloma pathology induced by Schistosoma japonicum infection. We found that IL-33 induced the increased production of IL-5 and IL-13 from splenocytes and liver mononuclear cells (MNCs) of infected mice. The infected mice developed significantly higher number of ST2L-expressing cells in spleen and liver. Most of the ST2L-expressing cells in liver were F4/80(+) macrophages, indicating the key role of macrophages in the response to IL-33. However, the liver MNCs in male-only worm infection had a poor response to IL-33, though elevated serum IL-33 was observed. ST2L(+)F4/80(+) cells were lower in male-only worm infection than that of mixed infection. IL-33 and soluble egg antigen (SEA) upregulated ST2L expression on macrophages in vitro and ST2L-expressing macrophage displayed MHCII(-)CD11b(+)M2 phenotype. Macrophage deletion significantly attenuated IL-33-induced type 2 immunity and egg granuloma formation during S. japonicum infection. These data demonstrate that IL-33 contributes to hepatic granuloma pathology through induction of M2 macrophages during S. japonicum infection.
authors.affiliation: Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education PRC, Guangzhou, 510080, China.|Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.affiliation: Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education PRC, Guangzhou, 510080, China.|Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, China.|School of Pharmaceutical Sciences, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, China.
authors.forename: Qixian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: The Third Affiliated Hospital of Sun Yet-sen University, Guangzhou, 510630, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education PRC, Guangzhou, 510080, China.|Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education PRC, Guangzhou, 510080, China.|Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Shen
authors.affiliation: Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education PRC, Guangzhou, 510080, China.|Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Sun
authors.affiliation: Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education PRC, Guangzhou, 510080, China.|Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.affiliation: Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education PRC, Guangzhou, 510080, China.|Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education PRC, Guangzhou, 510080, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education PRC, Guangzhou, 510080, China.|Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Feng
authors.affiliation: Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, 2011 Zonal Avenue, HMR 605, Los Angeles, CA 90033, USA.
authors.forename: Shuping
authors.initials: S
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education PRC, Guangzhou, 510080, China.|Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education PRC, Guangzhou, 510080, China.|Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, China.
authors.forename: Zhongdao
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D016764:Cell Polarity; D002478:Cells, Cultured; D005260:Female; D006099:Granuloma / Q000276:immunology / Q000469:parasitology; D006801:Humans; D000067596:Interleukin-33 / Q000502:physiology*; D008099:Liver / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology; D008264:Macrophages / Q000276:immunology* / Q000469:parasitology; D008297:Male; D008807:Mice, Inbred BALB C; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000378:metabolism; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000097:blood / Q000276:immunology* / Q000473:pathology; D015533:Transcriptional Activation
chemical_list: C506536:IL33 protein, human; D000067596:Interleukin-33; D017472:Receptors, Interleukin-1; C530318:ST2L protein, mouse