title: Relationship between elevated soluble CD74 and severity of experimental and clinical ALI/ARDS.
abstract: CD74 is expressed on the cell surface of pulmonary macrophages and contributes to macrophage migration inhibitory factor (MIF)-induced inflammatory response in acute lung injury (ALI). A circulating form of CD74 (soluble CD74, sCD74) was recently discovered in autoimmune liver disease. Using two murine ALI models and cells culture, we examined the presence of sCD74 in circulation and alveolar space and preliminarily assessed the biological function of sCD74. The concentrations of sCD74 were increased in serum and bronchoalveolar lavage fluids (BALF) of murine ALI models. The elevated levels of sCD74 in BALF positively correlated with lung permeability and inflammation. In addition, sCD74 is secreted by macrophages in response to MIF stimulation and itself can stimulate the production of inflammatory cytokines. Our clinical study confirmed some findings of basic research. Moreover, we also found Day 3 serum sCD74 levels were associated with worse clinical outcomes. In conclusion, higher serum sCD74 levels may reflect more severe lung injury and may be used to help physicians determine prognosis of acute respiratory distress syndrome (ARDS).
authors.affiliation: Department of Burn Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, P. R. China.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Burn Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Burn Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, P. R. China.
authors.forename: Kang'an
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Burn Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, P. R. China.
authors.forename: Zhengli
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Burn Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, P. R. China.
authors.forename: Xingtong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Burn Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, P. R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Chang
authors.affiliation: Department of Burn Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, P. R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Burn Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Feng
authors.affiliation: Department of Burn Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, P. R. China.
authors.forename: Zhaofan
authors.initials: Z
authors.lastname: Xia
mesh_terms: D055371:Acute Lung Injury / Q000175:diagnosis / Q000473:pathology*; D000328:Adult; D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis / Q000097:blood*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002460:Cell Line; D004195:Disease Models, Animal; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000097:blood*; D006801:Humans; D008297:Male; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D011379:Prognosis; D044967:Serum / Q000737:chemistry; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; C045084:invariant chain