title: Prevailing genotype distribution and characteristics of human respiratory syncytial virus in northeastern China.
abstract: Although human respiratory syncytial virus (RSV) is one of the most common viruses inducing respiratory tract infections in young children and the elderly, the genotype distribution and characteristics of RSV in northeastern China have not been investigated. Here, we identified 25 RSV-A and 8 RSV-B strains from 80 samples of patients with respiratory infections between February 2015 and May 2015. All 25 RSV-A viruses were classified as the ON1 genotype, which rapidly spread and became the dominant genotype in the world since being identified in Ontario (Canada) in December 2010. All eight RSV-B viruses belonged to the BA genotype with a 60-nucleotide duplication, seven of which formed two new genotypes, BA-CCA and BA-CCB. The remaining RSV-B virus clustered with one of the Hangzhou strains belonging to genotype BA11. Construction of a phylogenetic tree and amino acid substitution analysis showed that Changchun ON1 viruses exclusively constituted Lineages 3, 5 and 6, and contained several unique and newly identified amino acid substitutions, including E224G, R244K, L289I, Y297H, and L298P. Selective pressure was also evaluated, and various N and O-glycosylation sites were predicted. This study provides the first genetic analysis of RSV in northeastern China and may facilitate a better understanding of the evolution of this virus locally and globally. J. Med. Virol. 89:222-233, 2017. © 2016 The Authors. Journal of Medical Virology Published by Wiley Periodicals, Inc.
authors.affiliation: Department of Respiratory Medicine, First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Pediatric Respiratory Medicine, First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institute of Virology and AIDS Research, First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Virology and AIDS Research, First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Zhaolong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Virology and AIDS Research, First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Hou
authors.affiliation: Institute of Virology and AIDS Research, First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Jingliang
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Virology and AIDS Research, First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Yu
authors.affiliation: Institute of Virology and AIDS Research, First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Shucheng
authors.initials: S
authors.lastname: Hua
mesh_terms: D019943:Amino Acid Substitution; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010802:Phylogeny; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology* / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral
keywords: genetic variability; glycoproteins; mutation/mutation rate; respiratory syncytial virus