title: Structural investigation of the lipopolysaccharide O-chain isolated from Burkholderia fungorum strain DSM 17061.
abstract: Gram-negative bacteria exhibit lipopolysaccharides (LPSs) on their outer membrane surface. LPS is considered one of the most potent bacterial virulence factors. Here we report the elucidation of the LPS O-chain structure isolated from Burkholderia fungorum, a bacterium isolated from the white-rot fungus Phanerochaete chrysosporium that can act as a pathogen for plants and domesticated animals. The structure was determined by the employment of detailed chemical and NMR spectroscopy analyses as the following.
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126, Naples, Italy.
authors.forename: Antonia
authors.initials: A
authors.lastname: De Felice
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126, Naples, Italy.
authors.forename: Flaviana
authors.initials: F
authors.lastname: Di Lorenzo
authors.affiliation: Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI), Jena, Germany; Friedrich Schiller University, Jena, Germany.
authors.forename: Kirstin
authors.initials: K
authors.lastname: Scherlach
authors.affiliation: Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI), Jena, Germany; Friedrich Schiller University, Jena, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Ross
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126, Naples, Italy. Electronic address: silipo@unina.it.
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.affiliation: Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI), Jena, Germany; Friedrich Schiller University, Jena, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Hertweck
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126, Naples, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
mesh_terms: D019117:Burkholderia / Q000737:chemistry / Q000378:metabolism*; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry*
chemical_list: D008070:Lipopolysaccharides; D019081:O Antigens
keywords: Burkholderia fungorum; Lipopolysaccharide (LPS); NMR spectroscopy; O-chain; Structural characterization