title: Genome-wide DNA methylation profiling in zebrafish.
abstract: Genomic DNA methylation functions to repress gene expression by interfering with transcription factor binding and/or recruiting repressive chromatin machinery. Recent data support contribution of regulated DNA methylation to embryonic pluripotency, development, and tissue differentiation; this important epigenetic mark is chemically stable yet enzymatically reversible-and heritable through the germline. Importantly, all the major components involved in dynamic DNA methylation are conserved in zebrafish, including the factors that "write, read, and erase" this mark. Therefore, the zebrafish has become an excellent model for studying most biological processes associated with DNA methylation in mammals. Here we briefly review the zebrafish model for studying DNA methylation and describe a series of methods for performing genome-wide DNA methylation analysis. We address and provide methods for methylated DNA immunoprecipitation followed by sequencing (MeDIP-Seq), bisulfite sequencing (BS-Seq), and reduced representation bisulfite sequencing (RRBS-Seq).
authors.affiliation: University of Utah School of Medicine, Salt Lake City, UT, United States.
authors.forename: P J
authors.initials: PJ
authors.lastname: Murphy
authors.affiliation: University of Utah School of Medicine, Salt Lake City, UT, United States.
authors.forename: B R
authors.initials: BR
authors.lastname: Cairns
mesh_terms: D000818:Animals; D019175:DNA Methylation / Q000235:genetics*; D044127:Epigenesis, Genetic / Q000235:genetics*; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D015027:Zebrafish / Q000235:genetics
keywords: BS-Seq; Bisulfite; DNA methylation; Epigenetics; Genome-wide; MeDIP-Seq; RRBS-Seq; Reprogramming