title: A comparison of nanoscale and multiscale PCL/gelatin scaffolds prepared by disc-electrospinning.
abstract: Electrospinning is a versatile and convenient technology to generate nanofibers suitable for tissue engineering. However, the low production rate of traditional needle electrospinning hinders its applications. Needleless electrospinning is a potential strategy to promote the application of electrospun nanofiber in various fields. In this study, disc-electrospinning (one kind of needleless electrospinning) was conducted to produce poly(ε-caprolactone)/gelatin (PCL/GT) scaffolds of different structure, namely the nanoscale structure constructed by nanofiber and multiscale structure consisting of nanofiber and microfiber. It was found that, due to the inhomogeneity of PCL/GT solution, disc-electrospun PCL-GT scaffold presented multiscale structure with larger pores than that of the acid assisted one (PCL-GT-A). Scanning electron microscopy images indicated the PCL-GT scaffold was constructed by nanofibers and microfibers. Mouse fibroblasts and rat bone marrow stromal cells both showed higher proliferation rates on multiscale scaffold than nanoscale scaffolds. It was proposed that the nanofibers bridged between the microfibers enhanced cell adhesion and spreading, while the large pores on the three dimensional (3D) PCL-GT scaffold provide more effective space for cells to proliferate and migrate. However, the uniform nanofibers and densely packed structure in PCL-GT-A scaffold limited the cells on the surface. This study demonstrated the potential of disc-electrospun PCL-GT scaffold containing nanofiber and microfiber for 3D tissue regeneration.
authors.affiliation: Engineering Research Center of Technical Textiles, College of Textiles, Donghua University, Shanghai 201620, China; State Key Lab. for Modification of Chemical Fibers & Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Lab. for Modification of Chemical Fibers & Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Chen
authors.affiliation: State Key Lab. for Modification of Chemical Fibers & Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Sun
authors.affiliation: Engineering Research Center of Technical Textiles, College of Textiles, Donghua University, Shanghai 201620, China.
authors.forename: Haoxuan
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Lab. for Modification of Chemical Fibers & Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Wu
authors.affiliation: Engineering Research Center of Technical Textiles, College of Textiles, Donghua University, Shanghai 201620, China; Shanghai Normal University, Shanghai 200234, China.
authors.forename: Qinfei
authors.initials: Q
authors.lastname: Ke
authors.affiliation: Engineering Research Center of Technical Textiles, College of Textiles, Donghua University, Shanghai 201620, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt.
authors.forename: Hany
authors.initials: H
authors.lastname: Ei-Hamshary
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Salem S
authors.initials: SS
authors.lastname: Al-Deyab
authors.affiliation: Engineering Research Center of Technical Textiles, College of Textiles, Donghua University, Shanghai 201620, China; State Key Lab. for Modification of Chemical Fibers & Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China. Electronic address: xmm@dhu.edu.cn.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D057139:Nanofibers / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D005780:Gelatin
keywords: Acetic acid; Disc-electrospinning; Multiscale structure; Nanofiber; Three-dimensional scaffolds