title: Identification of a novel feline large granular lymphoma cell line (S87) as non-MHC-restricted cytotoxic T-cell line and assessment of its genetic instability.
abstract: Feline large granular lymphocyte lymphomas are rare but very aggressive tumors with a poor prognosis. In this study, a cell line from an abdominal effusion of a cat with large granular lymphoma was characterized. Immunophenotype staining was positive for CD3 and CD45R, and negative for CD4, CD8, CD56, CD79α, BLA.36 and NK1. A TCR γ gene rearrangement was detectable by PARR. Neither FeLV antigen nor exogenous FeLV provirus could be detected. A chromosomal instability associated with a centrosome hyperamplification could also be determined. The cell line is able to lyse target cells without antigen presentation or interaction with antigen presenting cells. Therefore, these cells were classified as genetically instable non-MHC-restricted cytotoxic T cells with large granular lymphocyte morphology.
authors.affiliation: Institute of Veterinary Pathology, Justus-Liebig Universtity Giessen, Frankfurter Strasse 96, 35392 Giessen, Germany. Electronic address: Lena.rydzewski@vetmed.uni-giessen.de.
authors.forename: Lena
authors.initials: L
authors.lastname: Rydzewski
authors.affiliation: Institute of Veterinary Pathology, Justus-Liebig Universtity Giessen, Frankfurter Strasse 96, 35392 Giessen, Germany.
authors.forename: Svenja
authors.initials: S
authors.lastname: Scheffold
authors.affiliation: Institute of Veterinary Pathology, Justus-Liebig Universtity Giessen, Frankfurter Strasse 96, 35392 Giessen, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Hecht
authors.affiliation: Institute of Veterinary Pathology, Justus-Liebig Universtity Giessen, Frankfurter Strasse 96, 35392 Giessen, Germany.
authors.forename: Eberhard
authors.initials: E
authors.lastname: Burkhardt
authors.affiliation: Institute of Infectious Disease of Animals, Justus-Liebig University Giessen, Frankfurter Strasse 85-89, 35392 Giessen, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Kerner
authors.affiliation: Veterinary Clinic for Obstetrics, Gynecology and Andrology Justus-Liebig Universtity Giessen, Frankfurter Strasse 106, 35392 Giessen,Germany.
authors.forename: Michele C
authors.initials: MC
authors.lastname: Klymiuk
authors.affiliation: Institute for Medical Psychology, Justus-Liebig University Giessen, Friedrichstrasse 36, Giessen, Germany.
authors.forename: Renate
authors.initials: R
authors.lastname: Deinzer
authors.affiliation: Institute of Veterinary Pathology, Justus-Liebig Universtity Giessen, Frankfurter Strasse 96, 35392 Giessen, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Reinacher
authors.affiliation: Institute of Veterinary Pathology, Justus-Liebig Universtity Giessen, Frankfurter Strasse 96, 35392 Giessen, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Henrich
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000235:genetics* / Q000276:immunology*; D002415:Cats / Q000235:genetics* / Q000276:immunology*; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D005260:Female; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D016158:Genes, p53; D042822:Genomic Instability; D016130:Immunophenotyping; D005256:Leukemia Virus, Feline / Q000302:isolation & purification; D008223:Lymphoma / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D046529:Microscopy, Electron, Transmission; D012333:RNA, Messenger / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D012333:RNA, Messenger
keywords: Centrosome amplification; Cytotoxic T cells; Genetic instability; Large granular lymphocyte; Lymphoma; p53