title: Multiple analytical methods for identification and quality evaluation of Fritillariae Thunbergii Bulbus based on biological single molecules by high-performance liquid chromatography.
abstract: Quantitative analysis for biological single molecules in Fritillariae Thunbergii Bulbus and identification method by chemometrics was established by using high-performance liquid chromatography. Amino acids, nucleosides, and nucleobases were quantitatively analyzed, and 11 peaks were selected for species identification. A Common pattern was established for chemometrics, and then similarity analysis, principal component analysis, and hierarchical cluster analysis heatmap were applied, and the results indicated that species were ideally identified from the adulterants as Fritillariae Cirrhosae Bulbus. This evaluation method was valuable for further quality control to select the characteristic components.
authors.affiliation: Suzhou Institute for Food and Drug Control, Suzhou, Jiangsu, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: National Institutes for Food and Drug Control, Beijing, China.|Xinjiang Institute for Food and Drug Control, Urumqi, Xinjiang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Suzhou Institute for Food and Drug Control, Suzhou, Jiangsu, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.affiliation: Suzhou Institute for Food and Drug Control, Suzhou, Jiangsu, China.
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Su
authors.affiliation: Suzhou Institute for Food and Drug Control, Suzhou, Jiangsu, China.
authors.forename: Hua-Feng
authors.initials: HF
authors.lastname: Zhang
authors.affiliation: Suzhou Institute for Food and Drug Control, Suzhou, Jiangsu, China. gu_bingren@yeah.net.
authors.forename: Bing-Ren
authors.initials: BR
authors.lastname: Gu
authors.affiliation: Suzhou Institute for Food and Drug Control, Suzhou, Jiangsu, China.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Xing
authors.affiliation: Suzhou Institute for Food and Drug Control, Suzhou, Jiangsu, China.
authors.forename: Man
authors.initials: M
authors.lastname: Xue
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D031410:Fritillaria / Q000737:chemistry*; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Chemometrics; Fingerprint analysis; Fritillariae Thunbergii Bulbus; High-performance liquid chromatography; Traditional Chinese medicine