title: Resveratrol prevents oxidative damage and loss of sperm motility induced by long-term treatment with valproic acid in Wistar rats.
abstract: Valproic acid (VPA) is a drug widely use for the treatment of epilepsy in both children and adults. Evidence suggests that long-term use of VPA may lead to an impairment in the male reproductive function. Oxidative stress is considered to play a major role in VPA associated toxicity. In the present work, we demonstrated that the natural antioxidant compound resveratrol (RSV) can be use to prevent VPA oxidative damage. Wistar rats treated with VPA (400mgkg(-1)) by gavage for 28days showed decrease in sperm motility accompanied by increase in oxidative damage to lipids and proteins. Additionally, VPA administration leaded to depletion of reduced glutathione and decrease in total antioxidant potential in testes and epididymides of Wistar rats. The co-administration of RSV (10mgkg(-1)) efficiently prevented VPA pro-oxidant effects. In summary, RSV was shown to protect the reproductive system from the damage induced by VPA. Altogether, our data strongly suggests that RSV administration might be a valuable strategy to minimize reproductive impairment in patients requiring long-term VPA treatment.
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS 97105-900, Brazil.
authors.forename: Giovana M
authors.initials: GM
authors.lastname: Ourique
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS 97105-900, Brazil.
authors.forename: Tanise S
authors.initials: TS
authors.lastname: Pês
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS 97105-900, Brazil.
authors.forename: Etiane M H
authors.initials: EM
authors.lastname: Saccol
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS 97105-900, Brazil.
authors.forename: Isabela A
authors.initials: IA
authors.lastname: Finamor
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction-BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS 97105-900, Brazil.
authors.forename: Werner G
authors.initials: WG
authors.lastname: Glanzner
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS 97105-900, Brazil.
authors.forename: Bernardo
authors.initials: B
authors.lastname: Baldisserotto
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS 97105-900, Brazil.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Pavanato
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction-BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS 97105-900, Brazil.
authors.forename: Paulo B D
authors.initials: PB
authors.lastname: Gonçalves
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS 97105-900, Brazil. Electronic address: barreto.kp@gmail.com.
authors.forename: Kátia P
authors.initials: KP
authors.lastname: Barreto
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000633:toxicity*; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D005298:Fertility / Q000187:drug effects; D005837:Genitalia, Male / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D017208:Rats, Wistar; D000077185:Resveratrol; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*; D013739:Testosterone / Q000097:blood; D014635:Valproic Acid / Q000633:toxicity*
chemical_list: D000927:Anticonvulsants; D000975:Antioxidants; D013267:Stilbenes; D013739:Testosterone; D014635:Valproic Acid; D000077185:Resveratrol
keywords: Antiepileptic; Antioxidant; Epididymides; Male fertility; Oxidative stress; Testes