title: Antioxidative response of Lepidium sativum L. during assisted phytoremediation of Hg contaminated soil.
abstract: In this study, Lepidium sativum L. was used in repeated phytoextraction processes to remove Hg from contaminated soil, assisted by combined use of compost and iodide (KI). L. sativum L. is sensitive to changes in environmental conditions and has been used in environmental tests. Its short vegetation period and ability to accumulate heavy metals make it suitable for use in repeated phytoextraction. The antioxidant enzymatic system of the plant (catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD) and glutathione S-transferase (GST)) was analysed, to understand the effects of increasing Hg accumulation and translocation. Phytoextraction was repeated six times to decrease Hg contamination in soil, and the efficiency of each step was assessed. The results indicate that L. sativum L. is able to take up and accumulate Hg from contaminated soil. A corresponding increase in enzymatic antioxidants shows that the plant defence system is activated in response to Hg stress. Using compost and KI increases total Hg accumulation and translocation to the above-ground parts of L. sativum L. Repeating the process decreases Hg contamination in pot experiments in all variants of the process. The combined use of compost and KI during repeated phytoextraction increases the efficiency of Hg removal from contaminated soil.
authors.affiliation: Lodz University of Technology, Department of Biotechnology and Food Sciences, Institute of General Food Chemistry, Stefanowskiego 4/10, 90-924 Lodz, Poland. Electronic address: beata.smolinska@p.lodz.pl.
authors.forename: Beata
authors.initials: B
authors.lastname: Smolinska
authors.affiliation: Lodz University of Technology, Department of Biotechnology and Food Sciences, Institute of General Food Chemistry, Stefanowskiego 4/10, 90-924 Lodz, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Szczodrowska
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004787:Environmental Pollution; D031220:Lepidium sativum / Q000378:metabolism*; D008628:Mercury / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D012989:Soil Pollutants; D010088:Oxidoreductases; D008628:Mercury
keywords: Antioxidant enzymes; Assisted phytoextraction; Hg; Lepidium sativum L.