title: Arabidopsis AtERF014 acts as a dual regulator that differentially modulates immunity against Pseudomonas syringae pv. tomato and Botrytis cinerea.
abstract: ERF transcription factors play critical roles in plant immune responses. Here, we report the function of AtERF014, a nucleus-localized transcriptional activator, in Arabidopsis immunity. Expression of AtERF014 was induced by Pseudomonas syringae pv. tomato (Pst) and Botrytis cinerea (Bc). AtERF014-overexpressing (OE) plants displayed increased Pst resistance but decreased Bc resistance, whereas AtERF014-RNAi plants exhibited decreased Pst resistance but increased Bc resistance. After Pst infection, expression of salicylic acid (SA)-responsive genes AtPR1 and AtPR5 in AtERF014-OE plants and of a jasmonic acid/ethylene-responsive gene AtPDF1.2 in AtERF014-RNAi plants was intensified but expression of AtPDF1.2 in AtERF014-OE plants and of AtPR1 and AtPR5 in AtERF014-RNAi plants was weakened. After Bc infection, expression of AtPR1 and AtPR5 in AtERF014-OE plants was attenuated but expression of AtPR1, AtPR5 and AtPDF1.2 in AtERF014-RNAi plants was strengthened. Pathogen- and flg22-induced ROS burst, expression of PTI genes and SA-induced defense were partially suppressed in AtERF014-RNAi plants, whereas pathogen-induced ROS and flg22-induced immune response were strengthened in AtER014-OE plants. Altered expression of AtERR014 affected expression of pectin biosynthetic genes and pectin content in AtERF014-RNAi plants was decreased. These data demonstrate that AtERF014 acts as a dual regulator that differentially modulates immunity against Pst and Bc in Arabidopsis.
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Hong
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Li
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Fengming
authors.initials: F
authors.lastname: Song
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics*; D020171:Botrytis / Q000276:immunology / Q000472:pathogenicity; D003517:Cyclopentanes / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D023082:Defensins / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics* / Q000276:immunology; D005030:Ethylenes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D054883:Oxylipins / Q000378:metabolism; D010368:Pectins / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity / Q000235:genetics*; D044224:Pseudomonas syringae / Q000276:immunology / Q000472:pathogenicity; D020156:Salicylic Acid / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D004268:DNA-Binding Proteins; D023082:Defensins; D005030:Ethylenes; D054883:Oxylipins; D010368:Pectins; D020156:Salicylic Acid; D014157:Transcription Factors; C000627070:ERF014 protein, Arabidopsis; C487542:At5g44420 protein, Arabidopsis; C077262:PR-1 protein, Arabidopsis; C011006:jasmonic acid; C036216:ethylene