title: Do respiratory limitations affect metabolism of insect larvae before moulting? An empirical test at the individual level.
abstract: Recent data suggest that oxygen limitation may induce moulting in larval insects. This oxygen-dependent induction of moulting (ODIM) hypothesis stems from the fact that the tracheal respiratory system of insects grows primarily at moults, whereas tissue mass increases massively between moults. This may result in a mismatch between oxygen supply and demand at the end of each larval instar because oxygen demand of growing tissues exceeds the relatively fixed supply capacity of the respiratory system. The ODIM hypothesis predicts that, within larval instars, respiration and metabolic rates of an individual larva first increase with increasing body mass but eventually level off once the supply capacity of the tracheal system starts to constrain metabolism. Here, we provide the first individual-level test of this key prediction of the ODIM hypothesis. We use a novel methodology where we repeatedly measure respiration and metabolic rates throughout the penultimate- and final-instar larvae in the butterfly Pieris napi In the penultimate instar, respiration and metabolic rates gradually decelerated along with growth, supporting the ODIM hypothesis. However, respiration and metabolic rates increased linearly during growth in the final instar, contradicting the prediction. Moreover, our data suggest considerable variation among individuals in the association between respiration rate and mass in the final instar. Overall, the results provide partial support for the ODIM hypothesis and suggest that oxygen limitation may emerge gradually within a larval instar. The results also suggest that there may be different moult induction mechanisms in larva-to-larva moults compared with the final metamorphic moult.
authors.affiliation: Department of Zoology, Stockholm University, Stockholm SE-10691, Sweden sami.kivela@oulu.fi.
authors.forename: Sami M
authors.identifier: 0000-0002-6844-9168
authors.initials: SM
authors.lastname: Kivelä
authors.affiliation: Department of Zoology, Stockholm University, Stockholm SE-10691, Sweden.
authors.forename: Philipp
authors.initials: P
authors.lastname: Lehmann
authors.affiliation: Department of Zoology, Stockholm University, Stockholm SE-10691, Sweden.
authors.forename: Karl
authors.initials: K
authors.lastname: Gotthard
mesh_terms: D000818:Animals; D001481:Basal Metabolism; D001823:Body Composition; D049628:Body Size; D002080:Butterflies / Q000254:growth & development* / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D005260:Female; D007814:Larva / Q000378:metabolism; D016014:Linear Models; D008055:Lipids / Q000032:analysis; D018978:Molting / Q000502:physiology*; D012044:Regression Analysis; D012119:Respiration; D013217:Starvation / Q000378:metabolism; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D008055:Lipids; D014867:Water; D002245:Carbon Dioxide
keywords: Critical mass; Growth; Larval instar; Metabolic rate; Pieris napi; Respirometry