title: Multilocus Sequence Typing of Clinical Isolates of Burkholderia pseudomallei Collected in Hainan, a Tropical Island of Southern China.
abstract: Burkholderia pseudomallei is a gram-negative bacterium that causes melioidosis. In this study, we examined the sequence types (STs) of 60 clinical isolates from patients with melioidosis in Hainan, a tropical island in southern China. The 60 clinical isolates were resolved into 30 STs. Among the STs, ST562 was also reported in Australia, and ST90 was also reported in the United States, probably from a traveler who had previously visited Asia. In addition, six novel STs were found in this study, including ST1395, ST1396, ST1397, ST1398, ST1399, and ST1443, whereas the remaining STs were mostly shared with other southeast Asian regions. Phylogenetic analysis of 60 isolates conducted using the eBURST v3 software segregated the 30 STs into three groups and 18 singletons. Our study shows genetic diversity of 60 clinical isolates of B. pseudomallei in Hainan Island.
authors.affiliation: Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China.
authors.forename: Xu-Ming
authors.initials: XM
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Beijing, People's Republic of China. National Institute for Communicable Disease Control and Prevention, Beijing, People's Republic of China. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Zhou
authors.affiliation: Hainan Department, The General Hospital of the Chinese People's Liberation Army, Sanya, People's Republic of China.
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Kuang
authors.affiliation: Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China.
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Guo
authors.affiliation: Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China.
authors.forename: Tian-Jiao
authors.initials: TJ
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China. liu_lingli@126.com liwei@icdc.cn.
authors.forename: Ling-Li
authors.initials: LL
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Beijing, People's Republic of China. National Institute for Communicable Disease Control and Prevention, Beijing, People's Republic of China. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, People's Republic of China. liu_lingli@126.com liwei@icdc.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D000483:Alleles; D015373:Bacterial Typing Techniques; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D014644:Genetic Variation; D005838:Genotype; D005843:Geography; D006801:Humans; D062312:Islands; D008554:Melioidosis / Q000382:microbiology*; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D012984:Software