title: [Establishment of a screening method for AZF microdeletions by capillary technology and a clinical trial].
abstract: OBJECTIVE
  To establish an accurate, fast and simple screening method for AZF microdeletions using capillary technology and use it for clinical testing.
  
  
  METHODS
  For each pair of primers, the 5' end of either forward or reverse primer was labeled with a FAM, JOE or TAMRA fluorescence dyes to establish multiplex quantitative fluorescence PCR systems for the establishment of a screening method of Y chromosome AZF microdeletions by capillary technology. The detection of Y chromosome AZF microdeletion was carried out on 725 cases of non-obstructive azoospermia, oligospermia or asthenospermia.
  
  
  RESULTS
  A screening method for Y chromosome AZF microdeletions using capillary technology was established. Thirty eight cases of AZF microdeletions were found among 725 cases of non-obstructive azoospermia, oligospermia or asthenospermia, which gave a deletion rate of 5.24%. Y chromosomal microdeletions were found in 8.62% of the azoospermia group, 6.75% of the oligozoospermic group, and 2.23% of the asthenospermia group.
  
  
  CONCLUSIONS
  An accurate, fast and simple screening method of Y chromosome AZF microdeletions by capillary technology has been established, which may have an important clinical value.
authors.affiliation: Medical Genetics Center, Key Laboratory of Metabolic and Genetic Disease in Women and Children, Reproductive Center, Guangdong Women and Children' Hospital, Guangzhou, Guangdong 511442, China. Email: yinaiwa@vip.126.com.
authors.forename: Tianwen
authors.initials: T
authors.lastname: He
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.forename: Yichun
authors.initials: Y
authors.lastname: Zheng
authors.forename: Changbin
authors.initials: C
authors.lastname: Zhang
authors.forename: Aihua
authors.initials: A
authors.lastname: Yin
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics*; D002197:Capillary Action; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y; D006801:Humans; D007248:Infertility, Male; D008297:Male; D060885:Multiplex Polymerase Chain Reaction; D012729:Sex Chromosome Aberrations; D058533:Sex Chromosome Disorders of Sex Development / Q000175:diagnosis*