title: Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation.
abstract: Clinical islet transplantation achieves insulin independence in selected patients, yet current methods for extracting islets from their surrounding pancreatic matrix are suboptimal. The islet basement membrane (BM) influences islet function and survival and is a critical marker of islet integrity following rodent islet isolation. No studies have investigated the impact of islet isolation on BM integrity in human islets, which have a unique duplex structure. To address this, samples were taken from 27 clinical human islet isolations (donor age 41-59, BMI 26-38, cold ischemic time < 10 h). Collagen IV, pan-laminin, perlecan and laminin-α5 in the islet BM were significantly digested by enzyme treatment. In isolated islets, laminin-α5 (found in both layers of the duplex BM) and perlecan were lost entirely, with no restoration evident during culture. Collagen IV and pan-laminin were present in the disorganized BM of isolated islets, yet a significant reduction in pan-laminin was seen during the initial 24 h culture period. Islet cytotoxicity increased during culture. Therefore, the human islet BM is substantially disrupted during the islet isolation procedure. Islet function and survival may be compromised as a consequence of an incomplete islet BM, which has implications for islet survival and transplanted graft longevity.
authors.affiliation: Islet Transplant Research Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford, UK.
authors.forename: S E
authors.initials: SE
authors.lastname: Cross
authors.affiliation: Islet Transplant Research Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford, UK.
authors.forename: R H
authors.initials: RH
authors.lastname: Vaughan
authors.affiliation: Islet Transplant Research Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Willcox
authors.affiliation: Islet Transplant Research Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: McBride
authors.affiliation: Islet Transplant Research Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford, UK.
authors.forename: A A
authors.initials: AA
authors.lastname: Abraham
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.
authors.forename: B
authors.initials: B
authors.lastname: Han
authors.affiliation: Islet Transplant Research Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford, UK.
authors.forename: J D
authors.initials: JD
authors.lastname: Johnson
authors.affiliation: Islet Transplant Research Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford, UK.
authors.forename: E
authors.initials: E
authors.lastname: Maillard
authors.affiliation: Islet Transplant Research Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford, UK.
authors.forename: P A
authors.initials: PA
authors.lastname: Bateman
authors.affiliation: Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford, UK.
authors.forename: R D
authors.initials: RD
authors.lastname: Ramracheya
authors.affiliation: Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford, UK.
authors.forename: P
authors.initials: P
authors.lastname: Rorsman
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.
authors.forename: K E
authors.initials: KE
authors.lastname: Kadler
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Dunne
authors.affiliation: Islet Transplant Research Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Hughes
authors.affiliation: Islet Transplant Research Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK.|Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford, UK.
authors.forename: P R V
authors.initials: PR
authors.lastname: Johnson
mesh_terms: D000328:Adult; D001485:Basement Membrane / Q000378:metabolism*; D002469:Cell Separation; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000378:metabolism*; D005260:Female; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism*; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation; D007797:Laminin / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged
chemical_list: D024141:Collagen Type IV; D019812:Heparan Sulfate Proteoglycans; D007797:Laminin; D008565:Membrane Proteins; C097043:laminin alpha5; C071927:perlecan
keywords: basic (laboratory) research/science; clinical research/practice; islet isolation; islet transplantation; islets of Langerhans