title: Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars.
abstract: The aim of the present study was to provide reliable experimental evidence for the application of autologous skin fibroblasts (asFbs) in the repair of depressed scars. In the experiments, depressed trauma was induced in male Wistar rats, and fibroblasts were separated from the removed skin tissues to culture in medium.In vitrocultured asFbs were injected into the depressed scar sites of rats, and the repair function of asFbs in the depressed scars was then examined at the cellular and whole-animal levels. The expression levels of type I and type III collagen in the dermal layer of the skin injected with asFb cells were significantly higher, as compared with those of the control, and type I collagen expression was significantly higher compared with Type III. Re-injection of asFbs into the dermal layer of depressed scars can markedly improve their repair. These results may prove useful for skin repair in clinical settings.
authors.affiliation: Department of Pathophysiology, Chengde Medical College, Chengde, Hebei 067000, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Pathophysiology, Chengde Medical College, Chengde, Hebei 067000, P.R. China.
authors.forename: Yan-Chun
authors.initials: YC
authors.lastname: Liu
authors.affiliation: Department of Pathophysiology, Chengde Medical College, Chengde, Hebei 067000, P.R. China.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Shi
authors.affiliation: Department of Pathophysiology, Chengde Medical College, Chengde, Hebei 067000, P.R. China.
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Xie
authors.affiliation: Department of Pathophysiology, Chengde Medical College, Chengde, Hebei 067000, P.R. China.
authors.forename: Hai-Peng
authors.initials: HP
authors.lastname: Cui
authors.affiliation: Department of Pathophysiology, Chengde Medical College, Chengde, Hebei 067000, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Experimental Pharmacology and Toxicology, Medical School of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Xiang-Jun
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Department of Experimental Pharmacology and Toxicology, Medical School of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Ren
keywords: collagen; depressed scar; fibroblasts; skin