title: Amplified voltammetric detection of miRNA from serum samples of glioma patients via combination of conducting magnetic microbeads and ferrocene-capped gold nanoparticle/streptavidin conjugates.
abstract: MicroRNA (miRNA) plays a key regulatory role in many biological processes, emerging as an important biomarker for a large variety of cancer diseases. Employing gold nanoparticle (AuNP)-coated magnetic microbeads (AuNP-MMBs) as an immobilization matrix for higher loading density of hairpin-structured DNA probes and then ferrocene (Fc)-capped gold nanoparticle/streptavidin conjugates, amplified electrochemical assay of miRNA has been performed. In the presence of target miRNA, a novel assembly was formed via linking biotinylated hairpin DNA probe-covered AuNP-MMBs with Fc-capped gold nanoparticle/streptavidin conjugates and then collected by magnetic electrodes for voltammetric detection. The enlarged surface area, good conductivity of AuNP-MMBs and the multiple Fc tags on the electrode surface ensure high sensitivity of the method. The oxidation peak current of Fc tags is proportional to the concentrations of miRNA ranging from 5 fM to 100 fM, and a detection limit of 0.14 fM was achieved. The proposed assay is highly selective and reproducible, serving as a viable alternative for the detection of miRNA-182 from serum samples of glioma patients.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China.
authors.forename: Zhixuan
authors.initials: Z
authors.lastname: Lu
authors.affiliation: SunYat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong 510060, PR China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Tang
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China.
authors.forename: Daohong
authors.initials: D
authors.lastname: Wu
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Cancer Research Institute, Central South University, Changsha, Hunan 410013, PR China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Wu
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China; School of Materials Science and Engineering, Central South University, Changsha, Hunan 410083, PR China. Electronic address: yixinyao@csu.edu.cn.
authors.forename: Xinyao
authors.initials: X
authors.lastname: Yi
authors.affiliation: School of Materials Science and Engineering, Central South University, Changsha, Hunan 410083, PR China.
authors.forename: Hengfeng
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China. Electronic address: jxiuwang@csu.edu.cn.
authors.forename: Jianxiu
authors.initials: J
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D015342:DNA Probes / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D005296:Ferrous Compounds / Q000737:chemistry*; D005910:Glioma / Q000097:blood*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry; D000075163:Metallocenes; D035683:MicroRNAs / Q000032:analysis / Q000097:blood*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D005296:Ferrous Compounds; C000597776:MIRN1182 microRNA, human; D000075163:Metallocenes; D035683:MicroRNAs; D006046:Gold; D019809:Streptavidin; C004998:ferrocene
keywords: Fc-capped gold nanoparticle/streptavidin conjugates; Gold nanoparticle-coated magnetic microbeads; Hairpin DNA probe; Target miRNA; Voltammetry