title: In Vivo19F MR Imaging Cell Tracking of Inflammatory Macrophages and Site-specific Development of Colitis-associated Dysplasia.
abstract: Purpose To investigate whether the magnitude of in vivo fluorine 19 (19F) magnetic resonance (MR) imaging signal is associated with subsequent development of colitis-associated dysplasia after in situ fluorination of inflammatory macrophages in a mouse model of inflammatory bowel disease (IBD). Materials and Methods Experiments were approved by the institutional animal care and use committee. Mice in the experimental group (n = 10) were administered azoxymethane and dextran sulfate sodium to induce colitis-associated dysplasia. Five mice were in the noninduced control group. Animals were injected with a commercially available perfluorocarbon and were examined in vivo with an 11.7-T MR imager for up to 110 days. Colons were then harvested followed by high-spatial-resolution ex vivo MR imaging. Multiple colon segments with or without19F signal were histologically graded and were correlated with19F signal intensity by using a Spearman correlation test. The signal intensity in mice with colitis-associated dysplasia was compared with that in control mice with a two-tailed Mann-Whitney U test. Results Patchy distributions of19F signal intensity in the colon wall were seen on in vivo and ex vivo images, representing chronic inflammation as shown by immunohistochemistry. Histologic scores of inflammation and site-specific development of colitis-associated dysplasia in the descending colon showed good correlation with normalized19F signal intensity (r = 0.88, P = .033 for the ascending colon; r = 0.82, P = .006 for the descending colon). A significantly (P = .002) higher normalized19F signal-to-noise ratio was found at sites that developed dysplasia (mean, 0.58 ± 0.09 [standard deviation]) as compared with sites that did not (mean, 0.17 ± 0.22). Conclusion19F MR imaging cell tracking of macrophages can be used to assess local inflammation in a mouse model of IBD. The resulting local19F signal intensity, representing the magnitude of inflammation, has a positive correlation with the development of colitis-associated dysplasia.©RSNA, 2016 Online supplemental material is available for this article.
authors.affiliation: From the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (S.H.S., D.K.K., J.W.M.B.), Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research (S.H.S., D.K.K., J.W.M.B.), Department of Oncology (D.K.K., J.W.M.B.), Department of Biomedical Engineering (S.H.S., J.W.M.B.), and Department of Chemical & Biomolecular Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, 217 Traylor Bldg, 720 Rutland Ave, Baltimore, MD 21205.
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Shin
authors.affiliation: From the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (S.H.S., D.K.K., J.W.M.B.), Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research (S.H.S., D.K.K., J.W.M.B.), Department of Oncology (D.K.K., J.W.M.B.), Department of Biomedical Engineering (S.H.S., J.W.M.B.), and Department of Chemical & Biomolecular Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, 217 Traylor Bldg, 720 Rutland Ave, Baltimore, MD 21205.
authors.forename: Deepak K
authors.initials: DK
authors.lastname: Kadayakkara
authors.affiliation: From the Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering (S.H.S., D.K.K., J.W.M.B.), Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research (S.H.S., D.K.K., J.W.M.B.), Department of Oncology (D.K.K., J.W.M.B.), Department of Biomedical Engineering (S.H.S., J.W.M.B.), and Department of Chemical & Biomolecular Engineering (J.W.M.B.), The Johns Hopkins University School of Medicine, 217 Traylor Bldg, 720 Rutland Ave, Baltimore, MD 21205.
authors.forename: Jeff W M
authors.initials: JW
authors.lastname: Bulte
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D003092:Colitis / Q000000981:diagnostic imaging* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005462:Fluorine Radioisotopes; D007091:Image Processing, Computer-Assisted; D008264:Macrophages / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D059629:Signal-To-Noise Ratio
chemical_list: D005462:Fluorine Radioisotopes