title: Leishmania-based expression systems.
abstract: Production of therapeutic or medical recombinant proteins, such as monoclonal antibodies, proteins, or active enzymes, requires a highly efficient system allowing natural folding and perfect post-translation modifications of the expressed protein. These requirements lead to the generation of a variety of gene expression systems from bacteria to eukaryotes. To achieve the best form of eukaryotic proteins, two factors need to be taken into consideration: choosing a suitable organism to express the protein of interest, and selecting an efficient delivery system. For this reason, the expression of recombinant proteins in eukaryotic nonpathogenic Leishmania parasites is an interesting approach which meets both criteria. Here, new Leishmania-based expression systems are compared with current systems that have long histories in research and industry.
authors.affiliation: Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Pasteur Ave. No. 69, Tehran, 13164, Iran. Tahereh_t@yahoo.com.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Taheri
authors.affiliation: Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Pasteur Ave. No. 69, Tehran, 13164, Iran.
authors.forename: Negar
authors.initials: N
authors.lastname: Seyed
authors.affiliation: Division of Endocrinology, Diabetes, and Clinical Nutrition, University Hospital Zurich, Zurich, Switzerland.
authors.forename: Amir
authors.initials: A
authors.lastname: Mizbani
authors.affiliation: Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Pasteur Ave. No. 69, Tehran, 13164, Iran.
authors.forename: Sima
authors.initials: S
authors.lastname: Rafati
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D015870:Gene Expression / Q000235:genetics; D007891:Leishmania / Q000235:genetics* / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins
keywords: Expression protein systems; Leishmania; Nonpathogenic parasites