title: Effect of protein S-nitrosylation on autolysis and catalytic ability of μ-calpain.
abstract: The effect of S-nitrosylation on the autolysis and catalytic ability of μ-calpain in vitro in the presence of 50μM Ca(2 +) was investigated. μ-Calpain was incubated with different concentrations of nitric oxide donor S-nitrosoglutathione (GSNO) and subsequently reacted with purified myofibrils. Results showed that the amount of 80kDa μ-calpain subunit significantly decreased as GSNO increased from 0 to 300μM, but increases of GSNO to 300, 500 and 1000μM did not result in further inhibition. The catalytic ability of nitrosylated μ-calpain to degrade titin, nebulin, troponin-T and desmin was significantly reduced when the GSNO concentration was higher than 300μM. The cysteine residues of μ-calpain at positions 49, 351, 384, and 592 in the catalytic subunit and at 142 in small subunit were S-nitrosylated, which could be responsible for decreased μ-calpain activity. Thus, S-nitrosylation can negatively regulate the activation of μ-calpain resulting in decreased proteolytic ability on myofibrils.
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yupin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Mengqin
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Guanghong
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wangang.zhang@njau.edu.cn.
authors.forename: Wangang
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001329:Autolysis / Q000378:metabolism*; D002154:Calpain / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002384:Catalysis; D003893:Desmin / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D009124:Muscle Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D009210:Myofibrils / Q000378:metabolism; D017293:Protein S / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D059748:Proteolysis; D013552:Swine; D020107:Troponin T / Q000032:analysis / Q000235:genetics / Q000378:metabolism
chemical_list: D003893:Desmin; D009124:Muscle Proteins; D017293:Protein S; D020107:Troponin T; C035620:nebulin; D002154:Calpain; C449002:mu-calpain
keywords: Autolysis and proteolysis; Myofibril degradation; Nitric oxide; Protein S-nitrosylation; μ-Calpain