title: Cloning and Characterization of Ginsenoside-Hydrolyzing β-Glucosidase fromLactobacillus brevisThat Transforms Ginsenosides Rb1 and F2 into Ginsenoside Rd and Compound K.
abstract: The ginsenoside-hydrolyzing β-glucosidase gene (bgy2) was cloned fromLactobacillus brevis. We expressed this gene inEscherichia coliBL21(DE3), isolated the resulting protein, and then utilized the enzyme for the biotransformation of ginsenosides. Thebgy2 gene contains 2,223 bp, and encodes a protein of 741 amino acids that is a member of glycosyl hydrolase family 3. β-Glucosidase (Bgy2) cleaved the outer glucose moieties of ginsenosides at the C-20 position, and the inner glucose at the C-3 position. Under optimal conditions (pH 7.0, 30°C), we used 0.1 mg/ml Bgy2 in 20 mM sodium phosphate buffer (PBS) for enzymatic studies. In these conditions, 1.0 mg/ml ginsenoside Rb1 and ginsenoside F2 were converted into 0.59 mg/ml ginsenoside Rd and 0.72mg/ml compound K, with molar conversion productivities of 69% and 91%, respectively. In pharmaceutical and commercial industries, this recombinant Bgy2 would be suitable for producting ginsenoside Rd and compound K.
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, and Department of Chemistry, College of Science, Yanbian University, Yanji 133002, P.R. China.
authors.forename: Fei-Liang
authors.initials: FL
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, and Department of Chemistry, College of Science, Yanbian University, Yanji 133002, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, and Department of Chemistry, College of Science, Yanbian University, Yanji 133002, P.R. China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, and Department of Chemistry, College of Science, Yanbian University, Yanji 133002, P.R. China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Dong
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, and Department of Chemistry, College of Science, Yanbian University, Yanji 133002, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, and Department of Chemistry, College of Science, Yanbian University, Yanji 133002, P.R. China.
authors.forename: Songquan
authors.initials: S
authors.lastname: Wu
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, and Department of Chemistry, College of Science, Yanbian University, Yanji 133002, P.R. China.
authors.forename: Donghao
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, and Department of Chemistry, College of Science, Yanbian University, Yanji 133002, P.R. China.
authors.forename: Lin-Hu
authors.initials: LH
authors.lastname: Quan
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D036145:Ginsenosides / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D052196:Levilactobacillus brevis / Q000201:enzymology / Q000235:genetics*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001617:beta-Glucosidase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D036145:Ginsenosides; D011994:Recombinant Proteins; D001617:beta-Glucosidase
keywords: Ginsenoside; Lactobacillus brevis; transformation; β-glucosidase