title: Biomimetic Porous PLGA Scaffolds Incorporating Decellularized Extracellular Matrix for Kidney Tissue Regeneration.
abstract: Chronic kidney disease is now recognized as a major health problem, but current therapies including dialysis and renal replacement have many limitations. Consequently, biodegradable scaffolds to help repairing injured tissue are emerging as a promising approach in the field of kidney tissue engineering. Poly(lactic-co-glycolic acid) (PLGA) is a useful biomedical material, but its insufficient biocompatibility caused a reduction in cell behavior and function. In this work, we developed the kidney-derived extracellular matrix (ECM) incorporated PLGA scaffolds as a cell supporting material for kidney tissue regeneration. Biomimetic PLGA scaffolds (PLGA/ECM) with different ECM concentrations were prepared by an ice particle leaching method, and their physicochemical and mechanical properties were characterized through various analyses. The proliferation of renal cortical epithelial cells on the PLGA/ECM scaffolds increased with an increase in ECM concentrations (0.2, 1, 5, and 10%) in scaffolds. The PLGA scaffold containing 10% of ECM has been shown to be an effective matrix for the repair and reconstitution of glomerulus and blood vessels in partially nephrectomized mice in vivo, compared with only PLGA control. These results suggest that not only can the tissue-engineering techniques be an effective alternative method for treatment of kidney diseases, but also the ECM incorporated PLGA scaffolds could be promising materials for biomedical applications including tissue engineered scaffolds and biodegradable implants.
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology , Seoul 02792, Republic of Korea.
authors.forename: Eugene
authors.initials: E
authors.lastname: Lih
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology , Seoul 02792, Republic of Korea.|Department of Chemical and Biomolecular Engineering, Sogang University , Seoul 04107, Republic of Korea.
authors.forename: Ki Wan
authors.initials: KW
authors.lastname: Park
authors.affiliation: Department of Urology, School of Medicine, Kyungpook National University , Daegu 41566, Republic of Korea.
authors.forename: So Young
authors.initials: SY
authors.lastname: Chun
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University , Seoul 04107, Republic of Korea.
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Urology, School of Medicine, Kyungpook National University , Daegu 41566, Republic of Korea.
authors.forename: Tae Gyun
authors.initials: TG
authors.lastname: Kwon
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology , Seoul 02792, Republic of Korea.|Department of Biomedical Engineering, Korea University of Science and Technology , Daejeon 34113, Republic of Korea.
authors.forename: Yoon Ki
authors.initials: YK
authors.lastname: Joung
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology , Seoul 02792, Republic of Korea.|Department of Biomedical Engineering, Korea University of Science and Technology , Daejeon 34113, Republic of Korea.
authors.forename: Dong Keun
authors.initials: DK
authors.lastname: Han
mesh_terms: D000818:Animals; D032701:Biomimetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D005109:Extracellular Matrix; D007668:Kidney; D019344:Lactic Acid; D051379:Mice; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: biomimetic scaffold; decellularized extracellular matrix; ice particle leaching method; kidney tissue regeneration; poly(lactide-co-glycolide)