title: Focusing on probe-modified peptides: a quick and effective method for target identification.
abstract: A new and efficient method focusing on probe-modified peptides was developed to identify the target protein and modification site of a hit compound or a drug. This method exhibited high click conjugation efficiency and few false-positive results. The modification site further facilitated target validation, biological mechanism study and new indications exploration.
authors.affiliation: College of Life Sciences, Beijing Normal University, Beijing 100875, China and National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: Huan
authors.initials: H
authors.lastname: Sun
authors.affiliation: National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Life Sciences, Beijing Normal University, Beijing 100875, China and National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: Weijie
authors.initials: W
authors.lastname: Hou
authors.affiliation: National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: Fanqi
authors.initials: F
authors.lastname: Zeng
authors.affiliation: National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: Sisi
authors.initials: S
authors.lastname: Li
authors.affiliation: National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: Yongfen
authors.initials: Y
authors.lastname: Ma
authors.affiliation: National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn.
authors.forename: She
authors.initials: S
authors.lastname: Chen
authors.affiliation: National Institute of Biological Sciences (NIBS), Beijing 102206, China. zhangzhiyuan@nibs.ac.cn and Collaborative Innovation Center for Cancer Medicine, Beijing, 102206, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D057930:Click Chemistry; D006801:Humans; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*
chemical_list: D015335:Molecular Probes; D010455:Peptides; D011506:Proteins