title: Expression of resistance gene analogs in woodland strawberry (Fragaria vesca) during infection with Phytophthora cactorum.
abstract: Important losses in strawberry production are often caused by the oomycete Phytophthora cactorum, the causal agent of crown rot. However, very limited studies at molecular levels exist of the mechanisms related to strawberry resistance against this pathogen. To begin to rectify this situation, a PCR-based approach (NBS profiling) was used to isolate strawberry resistance gene analogs (RGAs) with altered expression in response to P. cactorum during a time course (2, 4, 6, 24, 48, 96 and 192 h post-infection). Twenty-three distinct RGA fragments of the NB-LRR type were identified from a resistance genotype (Bukammen) of the wild species Fragaria vesca. The gene transcriptional profiles after infection showed that the response of most RGAs was quicker and stronger in the resistance genotype (Bukammen) than in the susceptible one (FDP821) during the early infection stage. The transcriptional patterns of one RGA (RGA109) were further monitored and compared during the P. cactorum infection of two pairs of resistant and susceptible genotype combinations (Bukammen/FDP821 and FDR1218/1603). The 5' end sequence was cloned, and its putative protein was characteristic of NBS-LRR R protein. Our results yielded a first insight into the strawberry RGAs responding to P. cactorum infection at molecular level.
authors.affiliation: Norwegian Institute of Bioeconomy Research, Box 115, 1431, Ås, Norway.|College of Horticulture and Plant Protection, Yangzhou University, Wenhui Eastern Road 48, Yangzhou, 225009, Jiangsu Province, China.
authors.forename: Xiao-Ren
authors.initials: XR
authors.lastname: Chen
authors.affiliation: Norwegian Institute of Bioeconomy Research, Box 115, 1431, Ås, Norway.
authors.forename: May Bente
authors.initials: MB
authors.lastname: Brurberg
authors.affiliation: Norwegian Institute of Bioeconomy Research, Box 115, 1431, Ås, Norway.
authors.forename: Abdelhameed
authors.initials: A
authors.lastname: Elameen
authors.affiliation: Norwegian Institute of Bioeconomy Research, Box 115, 1431, Ås, Norway.
authors.forename: Sonja Sletner
authors.initials: SS
authors.lastname: Klemsdal
authors.affiliation: Norwegian Institute of Bioeconomy Research, Box 115, 1431, Ås, Norway. inger.martinussen@nibio.no.
authors.forename: Inger
authors.initials: I
authors.lastname: Martinussen
mesh_terms: D003001:Cloning, Molecular; D060467:Disease Resistance; D031985:Fragaria / Q000235:genetics / Q000469:parasitology*; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D010838:Phytophthora / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D010940:Plant Proteins
keywords: Crown rot disease; NBS-LRR genes; Phytophthora cactorum; Resistance gene analogs; Strawberry