title: High-throughput label-free image cytometry and image-based classification of live Euglena gracilis.
abstract: We demonstrate high-throughput label-free single-cell image cytometry and image-based classification of Euglena gracilis (a microalgal species) under different culture conditions. We perform it with our high-throughput optofluidic image cytometer composed of a time-stretch microscope with 780-nm resolution and 75-Hz line rate, and an inertial-focusing microfluidic device. By analyzing a large number of single-cell images from the image cytometer, we identify differences in morphological and intracellular phenotypes between E. gracilis cell groups and statistically classify them under various culture conditions including nitrogen deficiency for lipid induction. Our method holds promise for real-time evaluation of culture techniques for E. gracilis and possibly other microalgae in a non-invasive manner.
authors.affiliation: Department of Chemistry, University of Tokyo, Tokyo, Japan; Department of Electronic Engineering, Tsinghua University, Beijing, China; leicheng@chem.s.u-tokyo.ac.jp.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lei
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan; Systems Biology Program, Graduate School of Media and Governance, Keio University, Fujisawa, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Ito
authors.affiliation: Department of Chemistry, University of Tokyo, Tokyo, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Ugawa
authors.affiliation: Department of Chemistry, University of Tokyo, Tokyo, Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Nozawa
authors.affiliation: euglena Co. Ltd., Tokyo, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Iwata
authors.affiliation: Graduate School of Advanced Integration Science, Chiba University, Chiba, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Maki
authors.affiliation: Graduate School of Advanced Integration Science, Chiba University, Chiba, Japan.
authors.forename: Genki
authors.initials: G
authors.lastname: Okada
authors.affiliation: Department of Chemistry, University of Tokyo, Tokyo, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kobayashi
authors.affiliation: Department of Chemistry, University of Tokyo, Tokyo, Japan; Department of Mechanical Engineering, Tsinghua University, Beijing, China.
authors.forename: Xinlei
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Chemistry, University of Tokyo, Tokyo, Japan; Department of Medicine, Thammasat University, Bangkok, Thailand.
authors.forename: Pimsiri
authors.initials: P
authors.lastname: Tiamsak
authors.affiliation: Graduate School of Advanced Integration Science, Chiba University, Chiba, Japan.
authors.forename: Norimichi
authors.initials: N
authors.lastname: Tsumura
authors.affiliation: euglena Co. Ltd., Tokyo, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Department of Bioengineering, University of California, Los Angeles, USA; California NanoSystems Institute, University of California, Los Angeles, USA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, USA.
authors.forename: Dino
authors.initials: D
authors.lastname: Di Carlo
authors.affiliation: Department of Electrical Engineering and Information Systems, University of Tokyo, Tokyo, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ozeki
authors.affiliation: Department of Chemistry, University of Tokyo, Tokyo, Japan; Department of Electrical Engineering, University of California, Los Angeles, USA; Japan Science and Technology Agency, Tokyo, Japan; goda@chem.s.u-tokyo.ac.jp.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Goda
keywords: (100.0100) Image processing; (110.0180) Microscopy; (120.0120) Instrumentation, measurement, and metrology