title: Histopathological and Radiographic Analysis of Dental Follicle of Impacted Teeth Using Modified Gallego's Stain.
abstract: BACKGROUND
  In the WHO classification of odontogenic tumours, hard tissue formation has been considered as a sub-classification however, this parameter has not been much explored in dental follicle in literature. Epithelial-mesenchymal interactions play an important role in odontogenesis and its associated pathologies; therefore research on dental follicle should also include mesenchymal components along with epithelial components. Additionally, special stains to identify the nature of such depositions in dental follicle have been less explored. Modified Gallego's stain is such an example which has not been tried in odontogenic lesions which makes this study unique.
  
  
  OBJECTIVE
  Aim of this study was to study histopathological variations in dental follicle, the nature of calcification and depositions using Modified Gallego's stain and to correlate histological features of dental follicle with pericoronal width radiographically.
  
  
  METHODS
  A prospective histological study of the dental follicles of 50 impacted teeth was carried out to microscopically evaluate the dental follicular tissues for pathological changes, and to correlate it with pericoronal radiolucency. Impacted teeth with pericoronal radiographic width less than 3mm were included in the study and symptomatic teeth were excluded. Further Modified Gallego stain was used to differentiate the nature of hard tissue formation in dental follicle tissues.
  
  
  RESULTS
  Dental follicle histologically showed pathological changes resembling dentigerous cyst, ameloblastoma, odontogenic fibroma (Simple and WHO Type), clear cell odontogenic tumour, neurofibroma, neurilemmoma and mucoepidermoid carcinoma.
  
  
  CONCLUSIONS
  The dental follicle surrounding an impacted tooth has the potential to differentiate into a wide variety of tissue types, and thus shows the potential for cyst and tumour development which was observed in this study in most of the specimens with normal follicular width radiographically.
authors.affiliation: Post Graduate Student, Department of Oral and Maxillofacial Pathology and Microbiology, Padmashree Dr D Y Patil Dental College and Hospital , Navi Mumbai, Maharashtra, India .
authors.forename: Evie
authors.initials: E
authors.lastname: Satheesan
authors.affiliation: Professor and PG Guide, Department of Oral and Maxillofacial Pathology and Microbiology, Padmashree Dr D Y Patil Dental College and Hospital , Navi Mumbai, Maharashtra, India .
authors.forename: Sandhya
authors.initials: S
authors.lastname: Tamgadge
authors.affiliation: Professor and HOD, Department of Oral and Maxillofacial Pathology and Microbiology, Padmashree Dr D Y Patil Dental College and Hospital , Navi Mumbai, Maharashtra, India .
authors.forename: Avinash
authors.initials: A
authors.lastname: Tamgadge
authors.affiliation: Professor and PG Guide, Department of Oral and Maxillofacial Pathology and Microbiology, Padmashree Dr D Y Patil Dental College and Hospital , Navi Mumbai, Maharashtra, India .
authors.forename: Sudhir
authors.initials: S
authors.lastname: Bhalerao
authors.affiliation: Professor and PG Guide, Department of Oral and Maxillofacial Pathology and Microbiology, Padmashree Dr D Y Patil Dental College and Hospital , Navi Mumbai, Maharashtra, India .
authors.forename: Treville
authors.initials: T
authors.lastname: Periera
keywords: Benign neoplasms; Dental follicle; Odontogenic cysts; Odontogenic tumours; Oral pathology; Pathologies; Radiographic width