title: Heat-shock protein 60 ofPorphyromonas gingivalismay induce dysfunction of human umbilical endothelial cells via regulation of endothelial-nitric oxide synthase and vascular endothelial-cadherin.
abstract: Accumulating evidence has established that periodontitis was an independent risk factor for coronary heart disease (CAD).Porphyromonus gingivalis(P. gingivalis), a major periodontal pathogen, has already been shown to have a significant role in the inflammatory response of CADin vivo. The aim of the present study was to identify whetherP. gingivalisheat-shock protein 60 (HSP60) induced the dysfunction of human umbilical vein endothelial cells (HUVECs)in vitro. HUVECs were stimulated with a range ofP. gingivalisHSP60 concentrations (1, 10 and 100 ng/l) at different time-points. The levels of vascular endothelial (VE)-cadherin, endothelial nitric oxide synthase (eNOS) and cysteinyl aspartate-specific protease-3 (caspase-3) were measured using western blot analysis. The apoptotic rate of HUVECs was detected using flow cytometry.P. gingivalisHSP60 at a concentration of 10 ng/l significantly decreased the expression levels of VE-cadherin and eNOS protein at 24 h stimulation, whereas no difference in these proteins was identified following a low dose ofP. gingivalisHSP60 (1 ng/l).P. gingivalisHSP60 at 100 ng/l significantly downregulated the expression levels of VE-cadherin and eNOS protein at 12 h in HUVECs. However, the cleavage of caspase-3 showed an opposing change at different concentrations. Consistently,P. gingivalisHSP60 induced apoptosis of HUVECs in a concentration-dependent manner. These results indicated thatP. gingivalisHSP60 may induce dysfunction and apoptosis in HUVECs via downregulating the expression levels of VE-cadherin and eNOS, and promoting the cleavage of caspase-3.
authors.affiliation: Department of Geratology, The Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China.
authors.forename: Cunjin
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Geratology, The Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China.
authors.forename: Yuanjie
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Department of Geratology, The Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China.
authors.forename: Limin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Geratology, The Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China.
authors.forename: Bainian
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Su
authors.affiliation: Department of Geratology, The Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
keywords: E-cadherin; Porphyromonas gingivalis; apoptosis; endothelial nitric oxide synthase; heat-shock protein