title: Neuropeptide Treatment with Cerebrolysin Enhances the Survival of Grafted Neural Stem Cell in an α-Synuclein Transgenic Model of Parkinson's Disease.
abstract: Neuronal stem cell (NSC) grafts have been investigated as a potential neuro-restorative therapy in Parkinson's disease (PD) but their use is compromised by the death of grafted cells. We investigated the use of Cerebrolysin (CBL), a neurotrophic peptide mixture, as an adjunct to NSC therapy in the α-synuclein (α-syn) transgenic (tg) model of PD. In vehicle-treated α-syn tg mice, there was decreased survival of NSCs. In contrast, CBL treatment enhanced the survival of NSCs in α-syn tg groups and ameliorated behavioral deficits. The grafted NSCs showed lower levels of terminal deoxynucleotidyl transferase dUTP nick end labeling positive cells in the CBL-treated mice when compared with vehicle-treated α-syn tg mice. No evidence of tumor growth was detected. Levels of α-syn were similar in the vehicle in CBL-treated tg mice. In conclusion, CBL treatment might be a potential adjuvant for therapeutic NSC grafting in PD.
authors.affiliation: Department of Neurosciences, University of California San Diego, La Jolla, CA, USA.
authors.forename: Edward
authors.initials: E
authors.lastname: Rockenstein
authors.affiliation: Department of Neurosciences, University of California San Diego, La Jolla, CA, USA.
authors.forename: Paula
authors.initials: P
authors.lastname: Desplats
authors.affiliation: Department of Neurosciences, University of California San Diego, La Jolla, CA, USA.
authors.forename: Kiren
authors.initials: K
authors.lastname: Ubhi
authors.affiliation: Department of Neurosciences, University of California San Diego, La Jolla, CA, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Mante
authors.affiliation: Department of Neurosciences, University of California San Diego, La Jolla, CA, USA.
authors.forename: Jazmin
authors.initials: J
authors.lastname: Florio
authors.affiliation: Department of Neurosciences, University of California San Diego, La Jolla, CA, USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Adame
authors.affiliation: Clinical Research and Pharmacology, EVER Neuro Pharma GmbH, Unterach, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Winter
authors.affiliation: Clinical Research and Pharmacology, EVER Neuro Pharma GmbH, Unterach, Austria.
authors.forename: Hemma
authors.initials: H
authors.lastname: Brandstaetter
authors.affiliation: Clinical Research and Pharmacology, EVER Neuro Pharma GmbH, Unterach, Austria.
authors.forename: Dieter
authors.initials: D
authors.lastname: Meier
authors.affiliation: Clinical Research and Pharmacology, EVER Neuro Pharma GmbH, Unterach, Austria.
authors.forename: Herbert
authors.initials: H
authors.lastname: Moessler
authors.affiliation: Department of Neurosciences, University of California San Diego, La Jolla, CA, USA.; Department of Pathology, University of California San Diego, La Jolla, CA, USA.
authors.forename: Eliezer
authors.initials: E
authors.lastname: Masliah
keywords: Parkinson’s disease; dementia with Lewy bodies; neurotrophic factors; stem cells; α-synuclein