title: Design of Laccase-Metal Organic Framework-Based Bioelectrodes for Biocatalytic Oxygen Reduction Reaction.
abstract: Laccase in combination with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as a mediator is a well-known bioelectrocatalyst for the 4-electron oxygen reduction reactions (ORR). The present work deals with the first exploitation of mesoporous iron(III) trimesate-based metal organic frameworks (MOF) MIL-100(Fe) (MIL stands for materials from Institut Lavoisier) as a new and efficient immobilization matrix of laccase for the building up of biocathodes for ORR. First, the immobilization of ABTS in the pores of the MOF was studied by combining micro-Raman spectroscopy, X-ray powder diffraction (XRPD), and N2 porosimetry. The ABTS-MIL-100(Fe)-based modified electrode presents excellent properties in terms of charge transfer kinetics and ionic conductivity as well as a very stable and reproducible electrochemical response, showing that MIL-100(Fe) provides a suitable and stabilizing microenvironment for electroactive ABTS molecules. In a second step, laccase was further immobilized on the MIL-100(Fe)-ABTS matrix. The Lac-ABTS-MIL-100(Fe)-CIE bioelectrode presents a high electrocatalytic current density of oxygen reduction and a reproducible electrochemical response characterized by a high stability over a long period of time (3 weeks). These results constitute a significant advance in the field of laccase-based bioelectrocatalysts for ORR. According to our work, it appears that the high catalytic efficiency of Lac-ABTS-MIL-100(Fe) for ORR may result from a synergy of chemical and catalytic properties of MIL-100(Fe) and laccase.
authors.affiliation: CNRS UMR 8587 , Bd François Mitterrand, 91025 Evry, France.|Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement (LAMBE), Université Evry, Université Paris Saclay , Bd François Mitterrand, 91025 Evry, France.|Institut Lavoisier de Versailles, UMR CNRS 8180, Université de Versailles St Quentin en Yvelines, Université Paris Saclay , 45 Avenue des Etats-Unis 78035 Versailles Cedex, France.
authors.forename: Snehangshu
authors.initials: S
authors.lastname: Patra
authors.affiliation: Institut Lavoisier de Versailles, UMR CNRS 8180, Université de Versailles St Quentin en Yvelines, Université Paris Saclay , 45 Avenue des Etats-Unis 78035 Versailles Cedex, France.
authors.forename: Saad
authors.initials: S
authors.lastname: Sene
authors.affiliation: Institut de Chimie de Clermont-Ferrand, UMR-CNRS 6296, Clermont Université, Université Blaise Pascal , BP 10448 F-63000 Clermont-Ferrand, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Mousty
authors.affiliation: Institut Lavoisier de Versailles, UMR CNRS 8180, Université de Versailles St Quentin en Yvelines, Université Paris Saclay , 45 Avenue des Etats-Unis 78035 Versailles Cedex, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Serre
authors.affiliation: CNRS UMR 8587 , Bd François Mitterrand, 91025 Evry, France.|Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement (LAMBE), Université Evry, Université Paris Saclay , Bd François Mitterrand, 91025 Evry, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Chaussé
authors.affiliation: CNRS UMR 8587 , Bd François Mitterrand, 91025 Evry, France.|Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement (LAMBE), Université Evry, Université Paris Saclay , Bd François Mitterrand, 91025 Evry, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Legrand
authors.affiliation: Institut Lavoisier de Versailles, UMR CNRS 8180, Université de Versailles St Quentin en Yvelines, Université Paris Saclay , 45 Avenue des Etats-Unis 78035 Versailles Cedex, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Steunou
keywords: ABTS; MIL-100(Fe); MOFs; bioelectrocatalysis; laccase; oxygen reduction reaction