title: Rapid development of xylanase assay conditions using Taguchi methodology.
abstract: The present investigation is mainly concerned with the rapid development of extracellular xylanase assay conditions by using Taguchi methodology. The extracellular xylanase was produced from Aspergillus niger (KP874102.1), a new strain isolated from a soil sample of the Baramura forest, Tripura West, India. Four physical parameters including temperature, pH, buffer concentration and incubation time were considered as key factors for xylanase activity and were optimized using Taguchi robust design methodology for enhanced xylanase activity. The main effect, interaction effects and optimal levels of the process factors were determined using signal-to-noise (S/N) ratio. The Taguchi method recommends the use of S/N ratio to measure quality characteristics. Based on analysis of the S/N ratio, optimal levels of the process factors were determined. Analysis of variance (ANOVA) was performed to evaluate statistically significant process factors. ANOVA results showed that temperature contributed the maximum impact (62.58%) on xylanase activity, followed by pH (22.69%), buffer concentration (9.55%) and incubation time (5.16%). Predicted results showed that enhanced xylanase activity (81.47%) can be achieved with pH 2, temperature 50°C, buffer concentration 50 Mm and incubation time 10 min.
authors.affiliation: a Department of Chemical Engineering , National Institute of Technology , Agartala , India.
authors.forename: Uma Shankar
authors.initials: US
authors.lastname: Prasad Uday
authors.affiliation: a Department of Chemical Engineering , National Institute of Technology , Agartala , India.
authors.forename: Tarun Kanti
authors.initials: TK
authors.lastname: Bandyopadhyay
authors.affiliation: b Department of Bio Engineering , National Institute of Technology , Agartala , India.
authors.forename: Biswanath
authors.initials: B
authors.lastname: Bhunia
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D001681:Biological Assay; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008962:Models, Theoretical; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D043364:Endo-1,4-beta Xylanases
keywords: Analysis of variance (ANOVA); Taguchi methodology; optimisation; signal-to-noise (S/N) ratio; xylanase; xylanase activity