title: Sinomenine inhibits A549 human lung cancer cell invasion by mediating the STAT3 signaling pathway.
abstract: Increasing evidence suggests that the failure of lung cancer treatment may occur as a result of tumor invasion and metastasis. Signal transducer and activator of transcription 3 (STAT3), an epithelial-mesenchymal transition-inducing transcription factor, is a key signaling molecule involved in the proliferation, apoptosis, invasion and metastasis of tumor cells. Sinomenine is an alkaloid compound with an antineoplastic potential against a variety of cancer cells. The aim of the present study was to assess the antitumor mechanisms of sinomenine in the A549 human lung cancer cell line. The results demonstrated that sinomenine manifested dose-dependent cytotoxicity and induced apoptosis in A549 cells. The protein expression of Janus kinase 2, STAT3, phosphorylated-STAT3, Snail, N-cadherin and vimentin decreased in sinomenine-treated cells, while E-cadherin protein expression increased. The regulation of STAT3, N-cadherin and E-cadherin by sinomenine was further confirmed by reverse transcription-quantitative polymerase chain reaction and immunofluorescent staining. It was demonstrated that sinomenine exerts inhibitory effects on A549 human lung cancer cell invasion, possibly through the inhibition of STAT3 signaling. These results provide a novel insight into the role of sinomenine in the treatment of non-small cell lung cancer.
authors.affiliation: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, P.R. China; Department of Oncology, Jining First People's Hospital, Jining, Shandong 272111, P.R. China.
authors.forename: Shulong
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, P.R. China; Beijing University of Chinese Medicine, Beijing 100029, P.R. China.
authors.forename: Yebo
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.affiliation: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.affiliation: Department of Oncology, Jining First People's Hospital, Jining, Shandong 272111, P.R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Oncology, Jining First People's Hospital, Jining, Shandong 272111, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, P.R. China.
authors.forename: Yanju
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, P.R. China.
authors.forename: Honggang
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, P.R. China.
authors.forename: Baojin
authors.initials: B
authors.lastname: Hua
keywords: epithelial-mesenchymal transition; lung cancer cell invasion; signal transducer and activator of transcription 3; sinomenine