title: Inflammations mediators and circulating levels of matrix metalloproteinases: Biomarkers of diabetes in Tunisians metabolic syndrome patients.
abstract: This study investigates the relationships between matrix metalloproteinases, inflammations mediators and type 2 diabetes mellitus in Tunisians metabolic syndrome (Mets) patients.
  
  
  
  The study has included 239 MetS patients and 247 controls. Mets was defined according to the NCEP-ATPIII report. Mets patients were also divided into two categories: 29 MetS non-diabetics and 210 MetS diabetics. Dysglycemia markers, matrix metalloproteinase-9 (MMP-9), Tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2), tumor necrosis factor α (TNF-α), C-reactive protein (CRP) levels and White Blood Cells (WBC) counts were determined in patients and controls.
  
  
  
  In our study, the level of inflammatory markers WBC, TNF-α and matrix metalloproteinases (MMP-8 and MMP-9) were significantly higher in diabetic patients with MetS, as compared with non-diabetic MetS patients. Inflammation mediators and MMP-9 were significantly associated with many clinical characteristics of MetS. The use of ROC "Receiver Operating Characteristic" analysis revealed the impact of TNF alpha on diabetes patients with MetS. In fact TNF alpha was found as a sensitive parameter in these patients with a sensitivity of 85%.
  
  
  
  Inflammation, matrix metalloproteinases and dysglycemia markers are not expressed in isolation but rather concurrently and are continuously interacting with each other, in MetS and diabetics patients. These markers fit with an early stage of cardiovascular disease (CVD); and measuring them could improve the risk evaluation, an early diagnosis, and the prognosis of CVD.
authors.affiliation: LR99ES11 Research Laboratory and Department of Biochemistry, Rabta Hospital, Manar University, Tunis, Tunisia.
authors.forename: Yosra
authors.initials: Y
authors.lastname: Zayani
authors.affiliation: LMBA, Department of Biology, Faculty of Sciences, El Manar University, Tunis, Tunisia.
authors.forename: Nargess
authors.initials: N
authors.lastname: El Golli
authors.affiliation: LR99ES11 Research Laboratory and Department of Biochemistry, Rabta Hospital, Manar University, Tunis, Tunisia.
authors.forename: Wiam
authors.initials: W
authors.lastname: Zidi
authors.affiliation: LR99ES11 Research Laboratory and Department of Biochemistry, Rabta Hospital, Manar University, Tunis, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Guizani
authors.affiliation: LR99ES11 Research Laboratory and Department of Biochemistry, Rabta Hospital, Manar University, Tunis, Tunisia.
authors.forename: Sameh
authors.initials: S
authors.lastname: Boussairi
authors.affiliation: LR99ES11 Research Laboratory and Department of Biochemistry, Rabta Hospital, Manar University, Tunis, Tunisia.
authors.forename: Sonia
authors.initials: S
authors.lastname: Aloui
authors.affiliation: LR99ES11 Research Laboratory and Department of Biochemistry, Rabta Hospital, Manar University, Tunis, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Ayadi
authors.affiliation: Department of Endocrinology, Rabta Hospital, El Manar University, Tunis, Tunisia.
authors.forename: Bochra
authors.initials: B
authors.lastname: Ftouhi
authors.affiliation: LR99ES11 Research Laboratory and Department of Biochemistry, Rabta Hospital, Manar University, Tunis, Tunisia.
authors.forename: Moncef
authors.initials: M
authors.lastname: Feki
authors.affiliation: Department of Hematology, Rabta Hospital, El Manar University, Tunis, Tunisia.
authors.forename: Neila
authors.initials: N
authors.lastname: Ben Romdhane
authors.affiliation: Department of Endocrinology, Rabta Hospital, El Manar University, Tunis, Tunisia.
authors.forename: Hedia
authors.initials: H
authors.lastname: Ben Sliman
authors.affiliation: LR99ES11 Research Laboratory and Department of Biochemistry, Rabta Hospital, Manar University, Tunis, Tunisia. Electronic address: monia_elasmi@yahoo.fr.
authors.forename: Monia
authors.initials: M
authors.lastname: Allal-Elasmi
authors.affiliation: LR99ES11 Research Laboratory and Department of Biochemistry, Rabta Hospital, Manar University, Tunis, Tunisia.
authors.forename: Naziha
authors.initials: N
authors.lastname: Kaabachi
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D002097:C-Reactive Protein / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000097:blood / Q000175:diagnosis; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000175:diagnosis* / Q000208:ethnology; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood; D018836:Inflammation Mediators / Q000097:blood*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood; D020782:Matrix Metalloproteinases / Q000097:blood*; D024821:Metabolic Syndrome / Q000097:blood* / Q000208:ethnology; D008875:Middle Aged; D012372:ROC Curve; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators; C560822:TIMP1 protein, human; C560823:TIMP2 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D014409:Tumor Necrosis Factor-alpha; D019716:Tissue Inhibitor of Metalloproteinase-2; D002097:C-Reactive Protein; D020782:Matrix Metalloproteinases; D020780:Matrix Metalloproteinase 9
keywords: Inflammation markers; Matrix metalloproteinases; Metabolic syndrome; Type 2 diabetes mellitus