title: Use of the potential probiotic strain Lactobacillus salivarius SMXD51 to control Campylobacter jejuni in broilers.
abstract: Campylobacteriosis is the most frequently reported zoonotic disease in humans in the EU since 2005. As chicken meat is the main source of contamination, reducing the level of Campylobacter in broiler chicken will lower the risk to consumers. The aim of this project was to evaluate the ability of Lactobacillus salivarius SMXD51 to control Campylobacter jejuni in broilers and to investigate the mechanisms that could be involved. Thirty broilers artificially contaminated with C. jejuni were treated by oral gavage with MRS broth or a bacterial suspension (107CFU) of Lb. salivarius SMXD51 (SMXD51) in MRS broth. At 14 and 35days of age, Campylobacter and Lb. salivarius loads were assessed in cecal contents. The impact of the treatment on the avian gut microbiota at day 35 was also evaluated. At day 14, the comparison between the control and treated groups showed a significant reduction (P<0.05) of 0.82 log. After 35days, a significant reduction (P<0.001) of 2.81 log in Campylobacter loads was observed and 73% of chickens treated with the culture exhibited Campylobacter loads below 7log10CFU/g. Taxonomic analysis revealed that SMXD51 treatment induced significant changes (P<0.05) in a limited number of bacterial genera of the avian gut microbiota and partially limited the impact of Campylobacter on Anaerotruncus sp. decrease and Subdoligranulum sp. increase. Thus, SMXD51 exhibits an anti-Campylobacter activity in vivo and can partially prevent the impact of Campylobacter on the avian gut microbiota.
authors.affiliation: SECALIM Unit UMR1014, Oniris, INRA, Université Bretagne Loire, Nantes, France.
authors.forename: Manuel Jimmy
authors.initials: MJ
authors.lastname: Saint-Cyr
authors.affiliation: SECALIM Unit UMR1014, Oniris, INRA, Université Bretagne Loire, Nantes, France.
authors.forename: Nabila
authors.initials: N
authors.lastname: Haddad
authors.affiliation: FARAH, Department of Food Sciences, Faculty of Veterinary Medicine, Liège University, Liège, Belgium.
authors.forename: Bernard
authors.initials: B
authors.lastname: Taminiau
authors.affiliation: Hygiene and Quality of Poultry and Pork Products Unit, ANSES, Ploufragan/Plouzané Laboratory, Université Bretagne Loire, Ploufragan, France.
authors.forename: Typhaine
authors.initials: T
authors.lastname: Poezevara
authors.affiliation: Hygiene and Quality of Poultry and Pork Products Unit, ANSES, Ploufragan/Plouzané Laboratory, Université Bretagne Loire, Ploufragan, France.
authors.forename: Ségolène
authors.initials: S
authors.lastname: Quesne
authors.affiliation: Department of Poultry Experimentation, ANSES, Ploufragan/Plouzané Laboratory, Université Bretagne Loire, Ploufragan, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Amelot
authors.affiliation: FARAH, Department of Food Sciences, Faculty of Veterinary Medicine, Liège University, Liège, Belgium.
authors.forename: Georges
authors.initials: G
authors.lastname: Daube
authors.affiliation: Hygiene and Quality of Poultry and Pork Products Unit, ANSES, Ploufragan/Plouzané Laboratory, Université Bretagne Loire, Ploufragan, France.
authors.forename: Marianne
authors.initials: M
authors.lastname: Chemaly
authors.affiliation: SECALIM Unit UMR1014, Oniris, INRA, Université Bretagne Loire, Nantes, France. Electronic address: xavier.dousset@oniris-nantes.fr.
authors.forename: Xavier
authors.initials: X
authors.lastname: Dousset
authors.affiliation: Hygiene and Quality of Poultry and Pork Products Unit, ANSES, Ploufragan/Plouzané Laboratory, Université Bretagne Loire, Ploufragan, France. Electronic address: muriel.guyard@anses.fr.
authors.forename: Muriel
authors.initials: M
authors.lastname: Guyard-Nicodème
mesh_terms: D000818:Animals; D002169:Campylobacter Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000502:physiology*; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D006801:Humans; D000069996:Ligilactobacillus salivarius / Q000502:physiology*; D011201:Poultry Diseases / Q000188:drug therapy* / Q000382:microbiology; D019936:Probiotics / Q000008:administration & dosage*
keywords: Avian gut microbiota; Broiler chickens; Campylobacter; Immune response; Lactobacillus