title: Insights into Abundant Rumen Ureolytic Bacterial Community Using Rumen Simulation System.
abstract: Urea, a non-protein nitrogen for dairy cows, is rapidly hydrolyzed to ammonia by urease produced by ureolytic bacteria in the rumen, and the ammonia is used as nitrogen for rumen bacterial growth. However, there is limited knowledge with regard to the ureolytic bacteria community in the rumen. To explore the ruminal ureolytic bacterial community, urea, or acetohydroxamic acid (AHA, an inhibitor of urea hydrolysis) were supplemented into the rumen simulation systems. The bacterial 16S rRNA genes were sequenced by Miseq high-throughput sequencing and used to reveal the ureoltyic bacteria by comparing different treatments. The results revealed that urea supplementation significantly increased the ammonia concentration, and AHA addition inhibited urea hydrolysis. Urea supplementation significantly increased the richness of bacterial community and the proportion of ureC genes. The composition of bacterial community following urea or AHA supplementation showed no significant difference compared to the groups without supplementation. The abundance of Bacillus and unclassified Succinivibrionaceae increased significantly following urea supplementation. Pseudomonas, Haemophilus, Neisseria, Streptococcus, and Actinomyces exhibited a positive response to urea supplementation and a negative response to AHA addition. Results retrieved from the NCBI protein database and publications confirmed that the representative bacteria in these genera mentioned above had urease genes or urease activities. Therefore, the rumen ureolytic bacteria were abundant in the genera of Pseudomonas, Haemophilus, Neisseria, Streptococcus, Actinomyces, Bacillus, and unclassified Succinivibrionaceae. Insights into abundant rumen ureolytic bacteria provide the regulation targets to mitigate urea hydrolysis and increase efficiency of urea nitrogen utilization in ruminants.
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesBeijing, China; Animal Science Unit, Gembloux Agro-Bio Tech, University of LiègeGembloux, Belgium.
authors.forename: Di
authors.initials: D
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Shengguo
authors.initials: S
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Dengpan
authors.initials: D
authors.lastname: Bu
authors.affiliation: Animal Science Unit, Gembloux Agro-Bio Tech, University of Liège Gembloux, Belgium.
authors.forename: Yves
authors.initials: Y
authors.lastname: Beckers
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
keywords: acetohydroxamic acid; high-throughput sequencing; rumen; urea; ureolytic bacteria