title: Chitinase Assay from Cultured Bone Marrow Derived Macrophages.
abstract: Chitinases are chitin-degrading enzymes. Chitinases play essential roles in combating chitin-containing pathogens as well as established roles in asthmatic inflammation. This assay is designed to detect chitinase activity in macrophage cell lysates. The chitin substrate is labeled with 4-methylumbelliferone. Hydrolysis of chitin releases 4-methylumbelliferone, and is measured fluorometrically to determine chitinase activity.
authors.affiliation: Division of Biomedical Sciences, University of California, Riverside, USA.
authors.forename: Danielle
authors.initials: D
authors.lastname: Worth
authors.affiliation: Division of Biomedical Sciences, University of California, Riverside, USA.
authors.forename: J Philip
authors.initials: JP
authors.lastname: Nance
authors.affiliation: Division of Biomedical Sciences, University of California, Riverside, USA.
authors.forename: Emma H
authors.initials: EH
authors.lastname: Wilson