title: Structure elucidation and gene cluster characterization of the O-antigen of Escherichia coli O80.
abstract: Mild alkaline degradation of the lipopolysaccharide of Escherichia coli O80 afforded a polysaccharide, which was studied by sugar analysis, selective cleavage of glycosidic linkages, and (1)H and (13)C NMR spectroscopy. Solvolysis of the polysaccharide with CF3CO2H cleaved the linkages of α-Fuc and β-linked GlcNAc and GalNAc residues to give two disaccharides. The following structure of the hexasaccharide repeating unit of the O-polysaccharide was established: The polysaccharide repeat also contains a minor O-acetyl group but its position was not determined. The O-antigen gene cluster of E. coli O80 between the conserved galF and gnd genes was analyzed and found to be consistent with the O-polysaccharide structure established.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.affiliation: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457, Tianjin, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Guo
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Filatov
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.affiliation: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457, Tianjin, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation. Electronic address: yknirel@gmail.com.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D019081:O Antigens
keywords: Bacterial polysaccharide structure; Escherichia coli; O-antigen; O-antigen gene cluster; O-polysaccharide