title: Clinical potential ofTCF21methylation in the diagnosis of renal cell carcinoma.
abstract: The aim of the present study was to investigate the clinical potential of transcription factor (TCF) 21 methylation in the diagnosis of renal cell carcinoma (RCC).TCF21methylation levels were quantified in renal tissues (55 cases of RCC tissue and 22 cases of normal tissue) and urine samples (33 cases of urine samples with RCC and 15 cases of normal urine samples) using pyrosequencing. Spearman's rank correlation coefficient was used to investigate the correlation betweenTCF21methylation levels and clinical parameters (gender, age, smoking history, Fuhrman grade and clinical stage). The receiver operating characteristic (ROC) curve was utilized to evaluate the accuracy of predictive diagnosis of RCC.TCF21methylation levels were significantly increased in RCC samples compared with normal renal tissues and urine samples. The Spearman's correlation analysis revealed that theTCF21methylation level was positively associated with age (P=0.002), smoking (P=0.017) and Fuhrman grade (P=0.045) in RCC tissues and was positively associated with tumor size (P<0.001), Fuhrman grade (P=0.017) and clinical stage (P=0.017) in urine samples. ROC curves revealed that the cut-off value, sensitivity and specificity were 23.61, 89.00 and 61.90%, respectively in tissue samples, and 26.84, 79 and 100%, respectively in urine samples. Furthermore, there were significant differences in the area under the curve between the tissue and urine samples (P=0.004). The results of the present study indicate thatTCF21may be used as a biomarker for diagnosing RCC, andTCF21methylation levels in urine samples may be a useful means of diagnosing RCC.
authors.affiliation: Department of Urology, The First Hospital of Quanzhou Affiliated Fujian Medical University, Quanzhou, Fujian 362000, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xin
authors.affiliation: Department of Pharmacy, Quanzhou Medical College, Quanzhou, Fujian 362011, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xu
authors.affiliation: Department of Urology, The First Hospital of Quanzhou Affiliated Fujian Medical University, Quanzhou, Fujian 362000, P.R. China.
authors.forename: Shaokun
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Urology, The First Hospital of Quanzhou Affiliated Fujian Medical University, Quanzhou, Fujian 362000, P.R. China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Xin
authors.affiliation: Department of Urology, The First Hospital of Quanzhou Affiliated Fujian Medical University, Quanzhou, Fujian 362000, P.R. China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Cai
authors.affiliation: Department of Urology, The First Hospital of Quanzhou Affiliated Fujian Medical University, Quanzhou, Fujian 362000, P.R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Urology, The First Hospital of Quanzhou Affiliated Fujian Medical University, Quanzhou, Fujian 362000, P.R. China.
authors.forename: Changde
authors.initials: C
authors.lastname: Fu
authors.affiliation: Department of Urology, General Hospital of the People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Guangfu
authors.initials: G
authors.lastname: Zhen
authors.affiliation: Department of Urology, The First Hospital of Quanzhou Affiliated Fujian Medical University, Quanzhou, Fujian 362000, P.R. China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Lai
authors.affiliation: Xiamen Xinchuang Bio-Technology Co. Ltd, Xiamen, Fujian 361021, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Xiamen Xinchuang Bio-Technology Co. Ltd, Xiamen, Fujian 361021, P.R. China.
authors.forename: Pengfeng
authors.initials: P
authors.lastname: Zhang
keywords: methylation level; pyrosequencing; renal cell carcinoma; transcription factor 21