title: Photo-triggered release from liposomes without membrane solubilization, based on binding to poly(vinyl alcohol) carrying a malachite green moiety.
abstract: When working with liposomes analogous to cell membranes, it is important to develop substrates that can regulate interactions with the liposome surface in response to light. We achieved a photo-triggered release from liposomes by using a copolymer of poly(vinyl alcohol) carrying a malachite green moiety (PVAMG). Although PVAMG is a neutral polymer under dark conditions, it is photoionized upon exposure to UV light, resulting in the formation of a cationic site for binding to liposomes with a negatively charged surface. Under UV irradiation, PVAMG showed effective interaction with liposomes, releasing the encapsulated compound; however, this release was negligible under dark conditions. The poly(vinyl alcohol) moiety of PVAMG played an important role in the photo-triggered release. This release was caused by membrane destabilization without lipid solubilization. We also investigated different aspects of liposome/PVAMG interactions, including PVAMG-induced fusion between the liposomes and the change in the liposome morphologies.
authors.affiliation: Department of Chemical Engineering, Nara National College of Technology, Yata 22, Yamato-koriyama, Nara 639-1080, Japan. Electronic address: ryoko@chem.nara-k.ac.jp.
authors.forename: Ryoko M
authors.initials: RM
authors.lastname: Uda
authors.affiliation: Department of Chemical Engineering, Nara National College of Technology, Yata 22, Yamato-koriyama, Nara 639-1080, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Department of Chemical Engineering, Nara National College of Technology, Yata 22, Yamato-koriyama, Nara 639-1080, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Takei
mesh_terms: D055592:Biophysical Phenomena; D002412:Cations; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D008566:Membranes / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D011143:Polyvinyl Chloride / Q000737:chemistry / Q000378:metabolism*; D012394:Rosaniline Dyes / Q000737:chemistry / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D002412:Cations; D008081:Liposomes; D011108:Polymers; D012394:Rosaniline Dyes; C005095:malachite green; D011143:Polyvinyl Chloride
keywords: Binding; Copolymer; Liposome; Malachite green; Photoionization; Release