title: Recombinant protein, AlnA, combined with transgenic alfalfa remediates polychlorinated biphenyl-contaminated soils: efficiency and rhizosphere microbial community response.
abstract: OBJECTIVE
  To investigate the remediation efficiency of polychlorinated biphenyl (PCB)-contaminated soils by the combination of a bioemulsifying protein, AlnA, and alfalfa expressing bphC.
  
  
  RESULTS
  The combination of AlnA and transgenic alfalfa promoted PCB soil remediation through the pot experiments. The removal rates of tri-PCBs (PCB 16/PCB 32 and PCB 31/PCB 28) and tetra-PCB (PCB 49) in transgenic alfalfa/AlnA treatment were 3.6-, 1.1-, and 2-fold higher than in transgenic alfalfa treatment alone. Analysis of gene copy number revealed that the PCB-degrading gene, bphC, of Pseudomonas-like bacterial populations in transgenic alfalfa/AlnA treatment increased 1.5-fold compared with that of unplanted soils. Bacterial community Illumina sequencing showed Pseudomonas, Arthrobacter, and Sphingomonas positively correlated with the removal rates of PCBs.
  
  
  CONCLUSIONS
  PCB removal was unrelated to bacterial community diversity but positively correlated with their specific degraders and bphC gene expression.
authors.affiliation: Key Laboratory of Groud Water Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun, 130021, Jilin, People's Republic of China.
authors.forename: Hejun
authors.initials: H
authors.lastname: Ren
authors.affiliation: Key Laboratory of Groud Water Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun, 130021, Jilin, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Plant Sciences, Jilin University, 5333, Xi'an Road, Changchun, 130062, Jilin, People's Republic of China.
authors.forename: Junxing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Plant Sciences, Jilin University, 5333, Xi'an Road, Changchun, 130062, Jilin, People's Republic of China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Pan
authors.affiliation: College of Plant Sciences, Jilin University, 5333, Xi'an Road, Changchun, 130062, Jilin, People's Republic of China.|Research Institute of Agricultural Resources and Environment, Key Laboratory of Plant Nutrition and Agro-Environment in Northeast Region, Jilin Academy of Agricultural Sciences, MOA, Changchun, Jilin, People's Republic of China.
authors.forename: Baoyu
authors.initials: B
authors.lastname: Chen
authors.affiliation: Key Laboratory of Groud Water Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun, 130021, Jilin, People's Republic of China. wang197911@163.com.|College of Plant Sciences, Jilin University, 5333, Xi'an Road, Changchun, 130062, Jilin, People's Republic of China. wang197911@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D000455:Medicago sativa / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000254:growth & development; D011078:Polychlorinated Biphenyls / Q000737:chemistry / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry / Q000302:isolation & purification
chemical_list: C443986:AlnA protein, Acinetobacter radioresistens; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D012989:Soil Pollutants; D011078:Polychlorinated Biphenyls; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase
keywords: AlnA, bioemulsifying protein; Illumina sequencing; Polychlorinated biphenyl; Transgenic alfalfa