title: A protocol for the culture and isolation of murine synovial fibroblasts.
abstract: The culture of synovial fibroblasts (SFs) is one of the most effective tools for investigating the pathology and physiology of synovial tissues and should prove useful for identifying the importance of SFs in disease as well as for the development of novel therapeutic approaches for several chronic joint diseases, such as rheumatoid arthritis. However, thus far, a detailed protocol for the primary culture and isolation of murine SFs has not been established. Therefore, the present study describes an easy and convenient method for isolating and culturing SFs from C57BL/6 mice. This protocol can be divided into 4 stages: Isolation of synovial tissues, isolation of SFs, seeding of SFs for growth in culture and purity analysis of SFs using the four cell markers, vimentin, cluster of differentiation 90.2 (CD90.2; Thy-1.2), intracellular adhesion molecule 1 (CD54) and vascular cell adhesion molecule 1 (CD106). This method is efficient and a purified population of SFs can be obtained 10 days after the initiation of culture.
authors.affiliation: Department of Rheumatology and Immunology, Nanfang Hospital, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Rheumatology and Immunology, Nanfang Hospital, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Ouyang
authors.affiliation: Research Center of Clinical Medicine, Nanfang Hospital, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Ziyou
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Rheumatology and Immunology, Nanfang Hospital, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Rheumatology and Immunology, Nanfang Hospital, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Rheumatology and Immunology, Nanfang Hospital, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Rheumatology and Immunology, Nanfang Hospital, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
keywords: C57BL/6 mouse; cell culture; isolation; rheumatoid arthritis; synovial fibroblasts