title: Ratiometric Detection of β-Amyloid and Discrimination from Lectins by a Supramolecular AIE Glyconanoparticle.
abstract: While the development of AIE (aggregation-induced-emission) based fluorimetric probes for biological applications has been an active research area, probes with a ratiometric signal for biomolecular recognition have been rare. Here, a ratiometric AIE glyconanoparticle formed by the supramolecular assembly between a silole-based AIEgen and fluorescent glycoprobes for the detection of amyloid β (Aβ) peptides and fibrils, which are a signature of neurological disorders such as the Alzheimer's disease, is shown. Complexation of glycoprobes with the AIEgen produces an intensive fluorescence emission of the former because of a Förster resonance energy transfer between the two molecules. Subsequently, the presence of Aβ dissembles the particle, producing a fluorescence emission of the AIEgen. Interestingly, the addition of lectins that selectively recognize the glycoprobes results in a different ratiometric response of the particle, thereby enabling a discrimination from Aβ detection. This research offers insight into the simple construction of multifunctional ratiometric probes based on the supramolecular hybridization of a wide variety of AIEgens with fluorescent molecular probes.
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, P. R. China.
authors.forename: Jun-Da
authors.initials: JD
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, P. R. China.
authors.forename: Ju
authors.initials: J
authors.lastname: Mei
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, P. R. China.
authors.forename: Xi-Le
authors.initials: XL
authors.lastname: Hu
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, P. R. China.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: He
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, P. R. China.
authors.forename: He
authors.initials: H
authors.lastname: Tian
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D037102:Lectins / Q000032:analysis / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology
chemical_list: D016229:Amyloid beta-Peptides; D005456:Fluorescent Dyes; D037102:Lectins
keywords: aggregation-induced-emission; fluorescence; fret; glyconanoparticles; lectin; β-amyloid