title: Primary cultures of human colon cancer as a model to study cancer stem cells.
abstract: The principal cause of death in cancer involves tumor progression and metastasis. Since only a small proportion of the primary tumor cells, cancer stem cells (CSCs), which are the most aggressive, have the capacity to metastasize and display properties of stem cells, it is imperative to characterize the gene expression of diagnostic markers and to evaluate the drug sensitivity in the CSCs themselves. Here, we have examined the key genes that are involved in the progression of colorectal cancer and are expressed in cancer stem cells. Primary cultures of colorectal cancer cells from a patient's tumors were studied using the flow cytometry and cytological methods. We have evaluated the clinical and stem cell marker expression in these cells, their resistance to 5-fluorouracil and irinotecan, and the ability of cells to form tumors in mice. The data shows the role of stem cell marker Oct4 in the resistance of primary colorectal cancer tumor cells to 5-fluorouracil.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, Tikhoretsky ave. 4, St. Petersburg, Russia, 194064. Koshkin31@mail.ru.
authors.forename: Sergey
authors.initials: S
authors.lastname: Koshkin
authors.affiliation: N.N. Petrov Oncology Research Institute, St. Petersburg, 197758, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Danilova
authors.affiliation: Russian Research Centre for Radiology and Surgical Technologies, St. Petersburg, 197758, Russia.
authors.forename: Grigory
authors.initials: G
authors.lastname: Raskin
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, Tikhoretsky ave. 4, St. Petersburg, Russia, 194064.
authors.forename: Nikolai
authors.initials: N
authors.lastname: Petrov
authors.affiliation: Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, St. Petersburg, 199034, Russia.|Department of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, 199034, Russia.
authors.forename: Olga
authors.initials: O
authors.lastname: Bajenova
authors.affiliation: Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, St. Petersburg, 199034, Russia.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: O'Brien
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, Tikhoretsky ave. 4, St. Petersburg, Russia, 194064.
authors.forename: Alexey
authors.initials: A
authors.lastname: Tomilin
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, Tikhoretsky ave. 4, St. Petersburg, Russia, 194064.
authors.forename: Elena
authors.initials: E
authors.lastname: Tolkunova
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002166:Camptothecin / Q000031:analogs & derivatives / Q000494:pharmacology; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005472:Fluorouracil / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D000077146:Irinotecan; D051379:Mice; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D000077146:Irinotecan; D005472:Fluorouracil; D002166:Camptothecin
keywords: 5-Fu; CD133; Cancer stem cells; Colon cancer; Drug resistance; Oct4