title: Biochemical composition of malignant ascites determines high aggressiveness of undifferentiated ovarian tumors.
abstract: Although undifferentiated tumors are the most lethal among all ovarian cancer histotypes, the exact reasons for this situation are unclear. This report was aimed at investigating whether the high aggressiveness of undifferentiated ovarian cancer may be associated with a biochemical composition of malignant ascites accumulating in the peritoneal cavity. We analyzed ascites from patients with undifferentiated, high-grade serous, endometrioid and clear-cell ovarian cancers, and from non-cancerous patients with respect to a group of soluble agents involved in cancer cell progression. Moreover, the effect of these fluids on proliferation and migration of ovarian cancer cells (A2780, OVCAR-3 and SKOV-3) was evaluated. The study showed that the level of all tested proteins in malignant ascites was higher than in the benign fluids. Concentration of 9/11 agents (CCL2, CXCL1, CXCL5, CXCL8, CXCL12, HGF, PAI-1, TGF-β1 and VEGF) was the greatest in the fluids from undifferentiated cancer, while the level of remaining 2 (IL-6 and uPA) was the highest in ascites from serous carcinoma. Proliferation of cancer cells was the most effective when they were subjected to ascites from patients with undifferentiated and serous cancer, whereas the migration was the highest in the case of undifferentiated tumors. Our findings indicate that the aggressiveness of undifferentiated ovarian tumors may be associated with the composition of malignant ascites, in particular the concentration of specific proinflammatory, cancer-promoting agents.
authors.affiliation: Department of Hypertensiology, Angiology and Internal Medicine, Poznań University of Medical Sciences, Długa 1/2 Str, 61-848, Poznan, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Mikuła-Pietrasik
authors.affiliation: Department of Hypertensiology, Angiology and Internal Medicine, Poznań University of Medical Sciences, Długa 1/2 Str, 61-848, Poznan, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Uruski
authors.affiliation: Division of Gynecological Surgery, Poznań University of Medical Sciences, Polna 33 Str, 60-535, Poznan, Poland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Szubert
authors.affiliation: Division of Gynecological Surgery, Poznań University of Medical Sciences, Polna 33 Str, 60-535, Poznan, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Moszyński
authors.affiliation: Division of Gynecological Surgery, Poznań University of Medical Sciences, Polna 33 Str, 60-535, Poznan, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Szpurek
authors.affiliation: Division of Gynecological Surgery, Poznań University of Medical Sciences, Polna 33 Str, 60-535, Poznan, Poland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Sajdak
authors.affiliation: Department of Hypertensiology, Angiology and Internal Medicine, Poznań University of Medical Sciences, Długa 1/2 Str, 61-848, Poznan, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Tykarski
authors.affiliation: Department of Hypertensiology, Angiology and Internal Medicine, Poznań University of Medical Sciences, Długa 1/2 Str, 61-848, Poznan, Poland. kksiazek@ump.edu.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Książek
mesh_terms: D001201:Ascites / Q000209:etiology / Q000378:metabolism*; D001202:Ascitic Fluid / Q000737:chemistry*; D005260:Female; D006801:Humans; D007118:Immunoassay; D010051:Ovarian Neoplasms / Q000473:pathology*
keywords: Cytokines; Malignant ascites; Ovarian cancer; Undifferentiated tumors