title: Isolation and identification of a new saponin from Cephalaria aytachii.
abstract: A new hederagenin-type triterpenoid glycoside (1) named Aytachoside A, along with eight known triterpene glycosides, were isolated from the aerial parts of Cephalaria aytachii Gokturk and Sumbul (Dipsacaceae). The structures of compounds 1-9 were determined by spectroscopic (1D and 2D NMR, HRESIMS) and chemical examinations. The antimicrobial effect of compound 1 was found considerably active against Escherichia coli, Pseudomonas aeruginosa and especially Salmonella typhimurium microorganisms using the MIC method. Although compound 1 was found not to have a remarkable toxic effect at a concentration lower than 300 μg/mL, cytotoxic activity tests demonstrated that prosapogenin 1a exhibits a significant cytotoxic activity against HeLa cell lines using the MTT assay for the first time.
authors.affiliation: a Faculty of Science, Department of Chemistry , Ege University , Izmir , Turkey.
authors.forename: Peyker
authors.initials: P
authors.lastname: Kayce
authors.affiliation: a Faculty of Science, Department of Chemistry , Ege University , Izmir , Turkey.
authors.forename: Suheyla
authors.initials: S
authors.lastname: Kirmizigul
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D049917:Dipsacaceae / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D004926:Escherichia coli / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects; D012503:Saponins / Q000737:chemistry* / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; C000618176:aytachoside A
keywords: Aytachoside A; Cephalaria aytachii; Dipsacaceae; antimicrobial activity; cytotoxic activity; triterpene saponin