title: Thymosin From Bombyx mori Is Down-Regulated in Expression by BmNPV Exhibiting Antiviral Activity.
abstract: Thymosins have been highly conserved during evolution. These hormones exist in many animal species and play an essential role in many biological events. However, little is known regarding the physiological function of silkworm Bombyx mori thymosin (BmTHY). In this study, we investigated the expression pattern of BmTHY in a Bombyx mori larval ovarian cell line (BmN) challenged with Bombyx mori nuclear polyhydrosis virus (BmNPV) and the antiviral effect of recombinant BmTHY (rBmTHY) for Bombyx mori against BmNPV. Western-blot assay and qRT-PCR analysis revealed that the level of BmTHY protein expression and transcription decreased over time when BmN cells were infected by BmNPV. Treatment with endotoxin-free rBmTHY led to a significant reduction in viral titer in the supernatant of BmN cells challenged with BmNPV. The results from antiviral tests performed in vitro and in vivo showed that endotoxin-free rBmTHY improved the survival rate of Bombyx mori infected with BmNPV. These findings suggest that BmTHY exerts immunomodulatory effects on Bombyx mori, rendering them resistant to viral infection.
authors.affiliation: College of Life Science, Institute of Biochemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China (zhangchen94911@126.com; 434700586@qq.com; fangqiangfight@sina.com; lmyywbb@sina.com; liaojx521@163.com; lisi214@126.com; wuxinzm@126. com; wenpingzhang@zstu.edu.cn).
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Life Science, Institute of Biochemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China (zhangchen94911@126.com; 434700586@qq.com; fangqiangfight@sina.com; lmyywbb@sina.com; liaojx521@163.com; lisi214@126.com; wuxinzm@126. com; wenpingzhang@zstu.edu.cn).
authors.forename: Yongdi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Science, Institute of Biochemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China (zhangchen94911@126.com; 434700586@qq.com; fangqiangfight@sina.com; lmyywbb@sina.com; liaojx521@163.com; lisi214@126.com; wuxinzm@126. com; wenpingzhang@zstu.edu.cn).
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, China (minlinxu@gmail.com).
authors.forename: Minlin
authors.initials: M
authors.lastname: Xu
authors.affiliation: College of Life Science, Institute of Biochemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China (zhangchen94911@126.com; 434700586@qq.com; fangqiangfight@sina.com; lmyywbb@sina.com; liaojx521@163.com; lisi214@126.com; wuxinzm@126. com; wenpingzhang@zstu.edu.cn).
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Lv
authors.affiliation: College of Life Science, Institute of Biochemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China (zhangchen94911@126.com; 434700586@qq.com; fangqiangfight@sina.com; lmyywbb@sina.com; liaojx521@163.com; lisi214@126.com; wuxinzm@126. com; wenpingzhang@zstu.edu.cn).
authors.forename: Jinxu
authors.initials: J
authors.lastname: Liao
authors.affiliation: College of Life Science, Institute of Biochemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China (zhangchen94911@126.com; 434700586@qq.com; fangqiangfight@sina.com; lmyywbb@sina.com; liaojx521@163.com; lisi214@126.com; wuxinzm@126. com; wenpingzhang@zstu.edu.cn).
authors.forename: Si
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Life Science, Institute of Biochemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China (zhangchen94911@126.com; 434700586@qq.com; fangqiangfight@sina.com; lmyywbb@sina.com; liaojx521@163.com; lisi214@126.com; wuxinzm@126. com; wenpingzhang@zstu.edu.cn) Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China.
authors.forename: Zuoming
authors.initials: Z
authors.lastname: Nie
authors.affiliation: College of Life Science, Institute of Biochemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China (zhangchen94911@126.com; 434700586@qq.com; fangqiangfight@sina.com; lmyywbb@sina.com; liaojx521@163.com; lisi214@126.com; wuxinzm@126. com; wenpingzhang@zstu.edu.cn) Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China wenpingzhang@zstu.edu.cn.
authors.forename: Wenping
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology*; D012831:Bombyx / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000821:virology*; D002460:Cell Line; D015536:Down-Regulation; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000187:drug effects / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D013947:Thymosin / Q000235:genetics* / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D019476:Insect Proteins; D011994:Recombinant Proteins; D013947:Thymosin
keywords: BmNPV; Bombyx mori; antivirus; thymosin