title: Secretory locations of SIPC in Amphibalanus amphitrite cyprids and a novel function of SIPC in biomineralization.
abstract: Settlement-inducing protein complex (SIPC) is a pheromone that triggers conspecific larval settlement in the barnacle Amphibalanus amphitrite. In the present study, immunostaining and scanning electron microscopy of SIPC revealed signals in the frontal horn pores and the secretions from carapace pores, suggesting that SIPC might be directly secreted from these organs in A. amphitrite cyprids. Further observations showed that the frontal horn pores could contact surfaces while cyprids were "walking". Immunostaining for SIPC on the contacted surfaces displayed SIPC signals. These signals were similar to the frontal horn pores in size and morphology, suggesting that frontal horn pores might deposit SIPC. Besides, full-length SIPC was expressed and subsequent assays indicated that recombinant SIPC was able to bind to chitins and induce the precipitation of CaCO3. Furthermore, recombinant SIPC inhibited the formation of vaterites and regulated the morphology of calcite crystals. The crystals that formed with recombinant SIPC were more stable against water erosion. Overall, these results reported a novel function of recombinant SIPC that regulates crystal formation in barnacle shells.
authors.affiliation: Environmental Science Programs and Division of Life Science, School of Science, the Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong S.A.R., P. R. China.
authors.forename: Gen
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Environmental Science Programs and Division of Life Science, School of Science, the Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong S.A.R., P. R. China.
authors.forename: Xiao-Xue
authors.initials: XX
authors.lastname: Yang
authors.affiliation: Environmental Science Programs and Division of Life Science, School of Science, the Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong S.A.R., P. R. China.
authors.forename: Pok Man
authors.initials: PM
authors.lastname: Leung
authors.affiliation: Sanya Institute of Deep-sea Science and Engineering, Chinese Academy of Science, No. 62, Fenghuang Road, Sanya, Hainan, 572000, P. R. China.
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: He
authors.affiliation: Environmental Science Programs and Division of Life Science, School of Science, the Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong S.A.R., P. R. China.
authors.forename: Tat Yin
authors.initials: TY
authors.lastname: Chan
authors.affiliation: Sanya Institute of Deep-sea Science and Engineering, Chinese Academy of Science, No. 62, Fenghuang Road, Sanya, Hainan, 572000, P. R. China.
authors.forename: Guo-Yong
authors.initials: GY
authors.lastname: Yan
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Science, Shenzhen University, Shenzhen, 518060, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Environmental Science Programs and Division of Life Science, School of Science, the Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong S.A.R., P. R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Sun
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Science, Shenzhen University, Shenzhen, 518060, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Environmental Science Programs and Division of Life Science, School of Science, the Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong S.A.R., P. R. China.
authors.forename: Pei-Yuan
authors.initials: PY
authors.lastname: Qian
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000378:metabolism; D001669:Biochemical Phenomena; D002113:Calcification, Physiologic; D002119:Calcium Carbonate / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry; D002686:Chitin / Q000737:chemistry; D002704:Chlorates / Q000737:chemistry; D003460:Crystallization; D005456:Fluorescent Dyes / Q000737:chemistry; D007313:Insecta; D007814:Larva / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D010675:Pheromones / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D013499:Surface Properties; D001468:Thoracica / Q000502:physiology*
chemical_list: D017610:Calcium Compounds; D002704:Chlorates; D005456:Fluorescent Dyes; D010675:Pheromones; D011994:Recombinant Proteins; D002686:Chitin; C091366:calcium chlorate; D002119:Calcium Carbonate