title: The influence of NBD fluorescent probe on model membranes containing POPC and DPPC.
abstract: To investigate the effect of fluorescent probe on the properties of membranes, we studied model membranes composed of 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl 2-oleoyl-sn-glycero-3-phosphocholine (POPC) in the presence and absence of fluorescent probe. The morphology of giant unilamellar vesicles (GUVs) has been observed as a function of temperature and composition by fluorescence microscopy using NBD-DOPE or C6-NBD-PC as the probe. The phase behavior of model membranes containing no fluorescent probe was investigated by2H-NMR spectroscopy. We found that the bright phase observed on GUVs was the fluid phase enriched in POPC and the dark phase was the gel phase enriched in DPPC. NBD-DOPE and C6-NBD-PC preferentially participated in the fluid-phase domains when GUVs were in the gel + fluid phase coexistence. Inclusion of both fluorescent probes (1 mol%) lowered the transition temperature of POPC/DPPC membranes. In addition, C6-NBD-PC exhibited a stronger effect than NBD-DOPE, which was considered to be associated with the structures of fluorescent molecules.
authors.affiliation: a Department of Physics , National Central University , Jhong-li , Taiwan.
authors.forename: Chi-Jung
authors.initials: CJ
authors.lastname: Weng
authors.affiliation: a Department of Physics , National Central University , Jhong-li , Taiwan.
authors.forename: Ju-Ping
authors.initials: JP
authors.lastname: Wu
authors.affiliation: a Department of Physics , National Central University , Jhong-li , Taiwan.
authors.forename: Ming-Yen
authors.initials: MY
authors.lastname: Kuo
authors.affiliation: a Department of Physics , National Central University , Jhong-li , Taiwan.
authors.forename: Ya-Wei
authors.initials: YW
authors.lastname: Hsueh
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D001393:Azoles / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D020404:Glycerophospholipids / Q000737:chemistry*; D005997:Glycerylphosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D008566:Membranes / Q000737:chemistry / Q000648:ultrastructure*; D009578:Nitrobenzenes / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D013696:Temperature; D053835:Unilamellar Liposomes / Q000737:chemistry
chemical_list: C505696:1-palm-itoyl-d31-2-oleoyl-sn-glycero-3-phosphocholine; C510797:7-nitrobenz-2-oxa-1,3-diazol-4-yl; D001393:Azoles; D005456:Fluorescent Dyes; D020404:Glycerophospholipids; D009578:Nitrobenzenes; D053835:Unilamellar Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; C081581:colfosceril palmitate; D005997:Glycerylphosphorylcholine
keywords: 2H NMR; C6-NBD-PC; DPPC; NBD-DOPE; POPC; fluorescence microscopy