title: Defining the Protein-Protein Interaction Network of the Human Protein Tyrosine Phosphatase Family.
abstract: Protein tyrosine phosphorylation, which plays a vital role in a variety of human cellular processes, is coordinated by protein tyrosine kinases and protein tyrosine phosphatases (PTPs). Genomic studies provide compelling evidence that PTPs are frequently mutated in various human cancers, suggesting that they have important roles in tumor suppression. However, the cellular functions and regulatory machineries of most PTPs are still largely unknown. To gain a comprehensive understanding of the protein-protein interaction network of the human PTP family, we performed a global proteomic study. Using a Minkowski distance-based unified scoring environment (MUSE) for the data analysis, we identified 940 high confidence candidate-interacting proteins that comprise the interaction landscape of the human PTP family. Through a gene ontology analysis and functional validations, we connected the PTP family with several key signaling pathways or cellular functions whose associations were previously unclear, such as the RAS-RAF-MEK pathway, the Hippo-YAP pathway, and cytokinesis. Our study provides the first glimpse of a protein interaction network for the human PTP family, linking it to a number of crucial signaling events, and generating a useful resource for future studies of PTPs.
authors.affiliation: From the ‡Department of Experimental Radiation Oncology, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030;
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: From the ‡Department of Experimental Radiation Oncology, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030;
authors.forename: Kim My
authors.initials: KM
authors.lastname: Tran
authors.affiliation: From the ‡Department of Experimental Radiation Oncology, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030;
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Aziz
authors.affiliation: From the ‡Department of Experimental Radiation Oncology, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030;
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Sorokin
authors.affiliation: From the ‡Department of Experimental Radiation Oncology, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030; jchen8@mdanderson.rg wenqiw6@uci.edu.
authors.forename: Junjie
authors.initials: J
authors.lastname: Chen
authors.affiliation: §Department of Developmental and Cell Biology, University of California, Irvine, California 92697 jchen8@mdanderson.rg wenqiw6@uci.edu.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Wang
mesh_terms: D063990:Gene Ontology; D053263:Gene Regulatory Networks; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D005810:Multigene Family; D060066:Protein Interaction Maps; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013379:Substrate Specificity
chemical_list: D017027:Protein Tyrosine Phosphatases