title: Secondary Metabolites Produced by an Endophytic Fungus Pestalotiopsis sydowiana and Their 20S Proteasome Inhibitory Activities.
abstract: Fungal endophytes have attracted attention due to their functional diversity. Secondary metabolites produced by Pestalotiopsis sydowiana from a halophyte, Phragmites communis Trinus, were investigated. Eleven compounds, including four penicillide derivatives (1-4) and seven α-pyrone analogues (5-10) were isolated from cultures of P. sydowiana. The compounds were identified based on spectroscopic data. The inhibitory activities against the 20S proteasome were evaluated. Compounds 1-3, 5, and 9-10 showed modest proteasome inhibition activities, while compound 8 showed strong activity with an IC50 of 1.2 ± 0.3 μM. This is the first study on the secondary metabolites produced by P. sydowiana and their proteasome inhibitory activities. The endophytic fungus P. sydowiana might be a good resource for proteasome inhibitors.
authors.affiliation: Key Biosensor Laboratory of Shandong Province, Biology Institute, Shandong Academy of Sciences, Jinan 250014, China. summerxkgao@163.com.
authors.forename: Xuekui
authors.initials: X
authors.lastname: Xia
authors.affiliation: National Institute of Biological Resources, Incheon 22689, Korea. sokim90@gmail.com.
authors.forename: Soonok
authors.initials: S
authors.lastname: Kim
authors.affiliation: Key Biosensor Laboratory of Shandong Province, Biology Institute, Shandong Academy of Sciences, Jinan 250014, China. changhengliu@163.com.
authors.forename: Changheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Duksung IDC, College of Pharmacy, Duksung Women's University, Seoul 01369, Korea. sangheeshim@duksung.ac.kr.
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Shim
mesh_terms: D001203:Ascomycota / Q000737:chemistry* / Q000378:metabolism; D001688:Biological Products / Q000737:chemistry* / Q000494:pharmacology*; D060026:Endophytes; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D046988:Proteasome Endopeptidase Complex; D061988:Proteasome Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D064210:Secondary Metabolism
chemical_list: D001688:Biological Products; D061988:Proteasome Inhibitors; D046988:Proteasome Endopeptidase Complex
keywords: 20S proteasome inhibitory activities; Pestalotiopsis sydowiana; secondary metabolites