title: Effects of geographic area, feedstock, temperature, and operating time on microbial communities of six full-scale biogas plants.
abstract: The objective of this study was to investigate the effect of different animal feedings operated in two distinct PDO (protected designation of origin) cheese production areas (Parmigiano Reggiano and Grana Padano) on the microbiome of six full-scale biogas plants, by means of Illumina sequencing and qPCR techniques. The effects of feedstock (cattle slurry manure, energy crops, agro-industrial by-products), temperature (mesophilic/thermophilic), and operating time were also examined, as were the relationships between the predominant bacterial and archaeal taxa and process parameters. The different feedstocks and temperatures strongly affected the microbiomes. A more biodiverse archaeal population was highlighted in Parmigiano Reggiano area plants, suggesting an influence of the different animal feedings. Methanosarcina and Methanosaeta showed an opposite distribution among anaerobic plants, with the former found to be related to ammonium concentration. The Methanoculleus genus was more abundant in the thermophilic digester whereas representation of the Thermotogales order correlated with hydraulic retention time.
authors.affiliation: Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, 29122 Piacenza, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Fontana
authors.affiliation: Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, 29122 Piacenza, Italy.
authors.forename: Vania
authors.initials: V
authors.lastname: Patrone
authors.affiliation: Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, 29122 Piacenza, Italy.
authors.forename: Edoardo
authors.initials: E
authors.lastname: Puglisi
authors.affiliation: Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, 29122 Piacenza, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Morelli
authors.affiliation: Centro Ricerche Biotecnologiche, Università Cattolica del Sacro Cuore, Via Milano, 24, 26100 Cremona, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Bassi
authors.affiliation: Centro Ricerche Produzioni Animali, C.R.P.A. S.p.A., Viale Timavo, 43/2, 42121 Reggio Emilia, Italy.
authors.forename: Mirco
authors.initials: M
authors.lastname: Garuti
authors.affiliation: Centro Ricerche Produzioni Animali, C.R.P.A. S.p.A., Viale Timavo, 43/2, 42121 Reggio Emilia, Italy.
authors.forename: Lorella
authors.initials: L
authors.lastname: Rossi
authors.affiliation: Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, 29122 Piacenza, Italy; Centro Ricerche Biotecnologiche, Università Cattolica del Sacro Cuore, Via Milano, 24, 26100 Cremona, Italy. Electronic address: fabrizio.cappa@unicatt.it.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Cappa
mesh_terms: D000821:Animal Feed; D000818:Animals; D056804:Biofuels / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology*; D002417:Cattle; D002611:Cheese; D008372:Manure; D013696:Temperature
chemical_list: D056804:Biofuels; D008372:Manure
keywords: 16S rRNA gene; Anaerobic digestion; Illumina sequencing; Metagenomics; Microbiome