title: Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV.
abstract: BACKGROUND
  Canine reticulated platelets (r-PLTs) i.e., juvenile PLTs reflecting thrombopoiesis can be measured automatically with the hematology analyzer Sysmex XT-2000iV using manual gating options. However, the impact of interferences on r-PLT measurements performed with the gates published previously (Pankraz et al., Vet Clin Path 38:30-38, 2009; Gelain et al., High fluorescent platelets fraction in macrothrombocytopenic Norfolk terrier, 2010) is largely unknown. The aim was to compare different published gates for measurement of r-PLTs with the Sysmex XT-2000iV with an own, optimized gate ("Oellers-gate") and to establish reference intervals (RIs) in > 120 dogs. Data of 362 measurements of diseased and healthy dogs were analyzed retrospectively. Several gates were applied and RIs for r-PLTs and platelet indices were established for pet dogs and a group of 153 healthy Beagles kept under defined housing conditions. Intra-assay precision (CV) was also assessed.
  
  
  RESULTS
  In 30/362 samples, interferences consistent with small erythrocytes/reticulocytes were seen in the previously published gates but not in the "Oellers-gate". Good correlation was found between the different gates (rs: 0.88-1.00). RIs for the "Pankraz-gate", the "Gelain-gate", and the "Oellers-gate" were 0.0-1.2, 0.2-3.7 and 0.2-3.9 % respectively. CVs were ranging between 22 and 41 %.
  
  
  CONCLUSIONS
  Optimization of previously published gates minimized interferences of small erythrocytes with r-PLT measurements.
authors.affiliation: Department of Veterinary Clinical Sciences, Clinical Pathophysiology and Clinical Pathology, Justus-Liebig University Giessen, Frankfurter Str. 126, 35392, Giessen, Germany. danaoellers@gmail.com.
authors.forename: Dana E
authors.initials: DE
authors.lastname: Oellers
authors.affiliation: Department of Veterinary Clinical Sciences, Clinical Pathophysiology and Clinical Pathology, Justus-Liebig University Giessen, Frankfurter Str. 126, 35392, Giessen, Germany.
authors.forename: Natali
authors.initials: N
authors.lastname: Bauer
authors.affiliation: IDEXX BioResearch Europe, A Division of IDEXX Laboratories, Ludwigsburg, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Ginder
authors.affiliation: Merck Serono, Global Non-Clinical Safety, Merck KGaA, Darmstadt, Germany.
authors.forename: Sigrid
authors.initials: S
authors.lastname: Johannes
authors.affiliation: Merck Serono, Global Non-Clinical Safety, Merck KGaA, Darmstadt, Germany.
authors.forename: Iris
authors.initials: I
authors.lastname: Pernecker
authors.affiliation: Department of Veterinary Clinical Sciences, Clinical Pathophysiology and Clinical Pathology, Justus-Liebig University Giessen, Frankfurter Str. 126, 35392, Giessen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Moritz
mesh_terms: D000818:Animals; D001772:Blood Cell Count / Q000295:instrumentation / Q000662:veterinary*; D001792:Blood Platelets / Q000145:classification* / Q000166:cytology*; D004285:Dogs / Q000097:blood*; D005260:Female; D008297:Male; D012016:Reference Values; D012680:Sensitivity and Specificity; D013045:Species Specificity
keywords: Canine; Hematology analyzer; RNA-rich platelets