title: Fast method for skeletal tissue gene expression analysis.
abstract: Several chronic diseases have been associated with bone alteration in the last few years. Despite the wealth of information provided by the analysis of the transcriptome in affected tissues, only a limited number of studies evaluated gene expression in bone tissue due to the difficulty to obtain high quality RNA. Therefore, skeletal pathologies have been often associated to a defective maturation process that occurs during recruitment of progenitor stem cells. In order to explore the possibility of analysing the gene expression during osteogenic differentiation in skeletal tissue, a single-step method to extract well-preserved RNA from bone specimens was performed. A comparison between this technique and a traditional method was made by analysing the quality and yield of RNA obtained. In addition, RNAs were assayed by reverse transcription-quantitative polymerase chain reaction to analyse the expression levels of the bone genes associated with the differentiation process in a mouse model. The present data showed that good quality RNA can be obtained from bone tissue by a simple single-step method allowing the expression analysis of the genes encoded by skeletal tissue. In conclusion, the present study allows the possibility to easily obtain good quality RNA from bone tissue that is suitable for gene expression studies of bone diseases.
authors.affiliation: Department of Medicine, Section of Internal Medicine D, University of Verona, I-37134 Verona, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Dalle Carbonare
authors.affiliation: Clinica Medica 1, Department of Medicine, University of Padova, I-35128 Padova, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Vilei
authors.affiliation: Department of Medicine, Section of Internal Medicine D, University of Verona, I-37134 Verona, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Stranieri
authors.affiliation: Department of Medicine, Section of Internal Medicine D, University of Verona, I-37134 Verona, Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Innamorati
authors.affiliation: Department of Surgery Oncology and Gastroenterology, University of Padova, I-35128 Padova, Italy; Veneto Institute of Oncology-IRCCS, I-35128 Padova, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Rosato
authors.affiliation: Clinica Medica 1, Department of Medicine, University of Padova, I-35128 Padova, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Boldrin
authors.affiliation: Clinica Medica 1, Department of Medicine, University of Padova, I-35128 Padova, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Sella
authors.affiliation: Clinica Medica 1, Department of Medicine, University of Padova, I-35128 Padova, Italy.
authors.forename: Sandro
authors.initials: S
authors.lastname: Giannini
authors.affiliation: Department of Medicine, Section of Internal Medicine D, University of Verona, I-37134 Verona, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Valenti
keywords: RNA; bone tissue; gene expression