title: Removal of Penicillin G and Erythromycin with Ionizing Radiation Followed by Biological Treatment.
abstract: The decomposition of penicillin G and erythromycin antibiotics at concentration of 0.2 mg ml(-1) by gamma irradiation at 50 kGy followed by biological treatment with Cupriavidus metallidurans CH34 was evaluated. Degradation of penicillin G and erythromycin was analyzed using nuclear magnetic resonance analysis (NMR), fourier transform infrared spectroscopy (FTIR), and chemical oxygen demand (COD). The exposure to the absorbed dose of 50 kGy caused degradation of penicillin G and erythromycin in the aqueous solution. The complete disappearance of NMR and FTIR peaks following irradiation confirmed the breakage of the β-lactam ring in penicillin G, and the decarboxylation and cleavage of the thiazolidine ring and for erythromycin, the complete destruction of the three aromatic rings. Irradiation alone removed 52.8 and 65.5 % of penicillin G and erythromycin, respectively. Further reduction to 12.6 and 14 % of the original penicillin G and erythromycin COD, respectively, was achieved using treatment of the irradiation products with C. metallidurans.
authors.affiliation: Laboratory of Biotechnology and Nuclear Technology, National Centre for Nuclear Science and Technology (CNSTN), Sidi Thabet Technopark, 2020, Ariana, Tunisia. issam.bensalem@cnstn.rnrt.tn.
authors.forename: Issam
authors.initials: I
authors.lastname: Ben Salem
authors.affiliation: Methods and Analysis Techniques Laboratory LR 10 INRAP 03, National Institute of Research and Physicochemical Analysis, 2020, Sidi Thabet, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Mezni
authors.affiliation: Laboratory of Natural Substances, National Institute of Research and Physico-chemical Analyses, Biotechnopole of Sidi Thabet, 2020, Ariana, Tunisia.
authors.forename: Abdennacer
authors.initials: A
authors.lastname: Boulila
authors.affiliation: Laboratory of Microbial Ecology and Technology LETMi-05ES08, National Institute of Applied Sciences and Technology, BP 676, 1080, Ariana, Tunisia.
authors.forename: Mokhtar
authors.initials: M
authors.lastname: Hamdi
authors.affiliation: Laboratory of Biotechnology and Nuclear Technology, National Centre for Nuclear Science and Technology (CNSTN), Sidi Thabet Technopark, 2020, Ariana, Tunisia.
authors.forename: Mouldi
authors.initials: M
authors.lastname: Saidi
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism; D001673:Biodegradation, Environmental; D049921:Cupriavidus / Q000378:metabolism* / Q000528:radiation effects; D004917:Erythromycin / Q000737:chemistry* / Q000378:metabolism; D005720:Gamma Rays; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010400:Penicillin G / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000900:Anti-Bacterial Agents; D004917:Erythromycin; D010400:Penicillin G