title: Directed differentiation of postnatal hippocampal neural stem cells generates nuclear receptor related‑1 protein‑ and tyrosine hydroxylase‑expressing cells.
abstract: Parkinson's disease (PD) is a severe neurodegenerative disorder. Although the detailed underlying molecular mechanism remains to be elucidated, the major pathological feature of PD is the loss of dopaminergic (DA) neurons of the substantia nigra. The use of donor stem cells to replace DA neurons may be a key breakthrough in the treatment of PD. In the present study, the growth kinetics of hippocampal neural stem cells (Hip‑NSCs) isolated from postnatal mice and cultured in vitro were observed, specifically the generation of cells expressing DA neuronal markers nuclear receptor related‑1 protein (Nurr1) and tyrosine hydroxylase (TH). It was revealed that Hip‑NSCs differentiated primarily into astrocytes when cultured in serum‑containing medium. However, in low serum conditions, the number of βIII tubulin‑positive neurons increased markedly. The proportion of Nurr1‑positive cells and TH‑positive neurons, significantly increased with increasing duration of directed differentiation of Hip‑NSCs (P=0.0187 and 0.0254, respectively). The results of the present study reveal that Hip‑NSCs may be induced to differentiate in vitro into neurons expressing Nurr1 and TH, known to be critical regulators of DA neuronal fate. Additionally, their expression may be necessary to facilitate neuronal maturation in vitro. These data suggest that Hip‑NSCs may serve as a source of DA neurons for cell therapy in patients diagnosed with PD.
authors.affiliation: Ningxia Key Laboratory of Cerebrocranial Diseases, Basic Medical College of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Yinxiu
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Radiotherapy, The General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Zixin
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Ningxia Key Laboratory of Cerebrocranial Diseases, Basic Medical College of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Cerebral Surgery, The General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Hechun
authors.initials: H
authors.lastname: Xia
authors.affiliation: Ningxia Key Laboratory of Cerebrocranial Diseases, Basic Medical College of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Ningxia Key Laboratory of Cerebrocranial Diseases, Basic Medical College of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Yinming
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Ningxia Key Laboratory of Cerebrocranial Diseases, Basic Medical College of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Quanrui
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Ningxia Key Laboratory of Cerebrocranial Diseases, Basic Medical College of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Ningxia Key Laboratory of Cerebrocranial Diseases, Basic Medical College of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000378:metabolism* / Q000502:physiology; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2 / Q000235:genetics*; D010300:Parkinson Disease; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics*
chemical_list: C539883:Nr4a2 protein, mouse; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D014446:Tyrosine 3-Monooxygenase