title: Transcriptomic Analysis of the Endangered Neritid Species Clithon retropictus: De Novo Assembly, Functional Annotation, and Marker Discovery.
abstract: An aquatic gastropod belonging to the family Neritidae, Clithon retropictus is listed as an endangered class II species in South Korea. The lack of information on its genomic background limits the ability to obtain functional data resources and inhibits informed conservation planning for this species. In the present study, the transcriptomic sequencing and de novo assembly of C. retropictus generated a total of 241,696,750 high-quality reads. These assembled to 282,838 unigenes with mean and N50 lengths of 736.9 and 1201 base pairs, respectively. Of these, 125,616 unigenes were subjected to annotation analysis with known proteins in Protostome DB, COG, GO, and KEGG protein databases (BLASTX; E ≤ 0.00001) and with known nucleotides in the Unigene database (BLASTN; E ≤ 0.00001). The GO analysis indicated that cellular process, cell, and catalytic activity are the predominant GO terms in the biological process, cellular component, and molecular function categories, respectively. In addition, 2093 unigenes were distributed in 107 different KEGG pathways. Furthermore, 49,280 simple sequence repeats were identified in the unigenes (>1 kilobase sequences). This is the first report on the identification of transcriptomic and microsatellite resources for C. retropictus, which opens up the possibility of exploring traits related to the adaptation and acclimatization of this species.
authors.affiliation: Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunghyangro, Shinchang-myeon, Asan, Chungcheongnam-do 31538, Korea. cindysory@naver.com.
authors.forename: So Young
authors.initials: SY
authors.lastname: Park
authors.affiliation: Trident School of Biotech Sciences, Trident Academy of Creative Technology (TACT), Chandaka Industrial Estate, Chandrasekharpur, Bhubaneswar, Odisha 751024, India. drbharatbhusan4@gmail.com.
authors.forename: Bharat Bhusan
authors.initials: BB
authors.lastname: Patnaik
authors.affiliation: Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunghyangro, Shinchang-myeon, Asan, Chungcheongnam-do 31538, Korea. bioksw@naver.com.
authors.forename: Se Won
authors.initials: SW
authors.lastname: Kang
authors.affiliation: Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunghyangro, Shinchang-myeon, Asan, Chungcheongnam-do 31538, Korea. hwamux@naver.com.
authors.forename: Hee-Ju
authors.initials: HJ
authors.lastname: Hwang
authors.affiliation: Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunghyangro, Shinchang-myeon, Asan, Chungcheongnam-do 31538, Korea. jong6922@daum.net.
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Chung
authors.affiliation: Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunghyangro, Shinchang-myeon, Asan, Chungcheongnam-do 31538, Korea. elegangce@naver.com.
authors.forename: Dae Kwon
authors.initials: DK
authors.lastname: Song
authors.affiliation: Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunghyangro, Shinchang-myeon, Asan, Chungcheongnam-do 31538, Korea. gabriermingu@naver.com.
authors.forename: Min Kyu
authors.initials: MK
authors.lastname: Sang
authors.affiliation: Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunghyangro, Shinchang-myeon, Asan, Chungcheongnam-do 31538, Korea. hhowrelia.patnaik@gmail.com.
authors.forename: Hongray Howrelia
authors.initials: HH
authors.lastname: Patnaik
authors.affiliation: Korea Zoonosis Research Institute (KOZRI), Chonbuk National University, 820-120 Hana-ro, Iksan, Jeollabuk-do 54528, Korea. jblee@jbnu.ac.kr.
authors.forename: Jae Bong
authors.initials: JB
authors.lastname: Lee
authors.affiliation: College of Agriculture and Life Science, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Korea. annemi@hanmail.net.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Noh
authors.affiliation: National Institute of Biological Resources, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea. snubull@korea.kr.
authors.forename: Changmu
authors.initials: C
authors.lastname: Kim
authors.affiliation: National Institute of Biological Resources, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea. sokim90@korea.kr.
authors.forename: Soonok
authors.initials: S
authors.lastname: Kim
authors.affiliation: Research Institute, GnC BIO Co., LTD., 621-6 Banseok-dong, Yuseong-gu, Daejeon 34069, Korea. 5022daniel@gmail.com.
authors.forename: Hong Seog
authors.initials: HS
authors.lastname: Park
authors.affiliation: Institute of Environmental Research, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do 243341, Korea. sljun@kangwon.ac.kr.
authors.forename: Jun Sang
authors.initials: JS
authors.lastname: Lee
authors.affiliation: College of Agriculture and Life Science, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Korea. hanys@chonnam.ac.kr.
authors.forename: Yeon Soo
authors.initials: YS
authors.lastname: Han
authors.affiliation: Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunghyangro, Shinchang-myeon, Asan, Chungcheongnam-do 31538, Korea. yslee@sch.ac.kr.
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Lee
keywords: Clithon retropictus; de novo assembly; functional annotation; simple sequence repeats; transcriptome