title: Radioprotective Effect of Grape Seed Proanthocyanidins In Vitro and In Vivo.
abstract: We have demonstrated that grape seed proanthocyanidins (GSPs) could effectively scavenge hydroxyl radical (•OH) in a dose-dependent manner. Since most of the ionizing radiation- (IR-) induced injuries were caused by •OH, this study was to investigate whether GSPs would mitigate IR-induced injuries in vitro and in vivo. We demonstrated that GSPs could significantly reduce IR-induced DNA strand breaks (DSBs) and apoptosis of human lymphocyte AHH-1 cells. This study also showed that GSPs could protect white blood cells (WBC) from IR-induced injuries, speed up the weight of mice back, and decrease plasma malondialdehyde (MDA), thus improving the survival rates of mice after ionizing radiation. It is suggested that GSPs have a potential as an effective and safe radioprotective agent.
authors.affiliation: Department of Radiology, The First Hospital of Jiaxing, 1882 Zhonghuan South Road, Jiaxing, Zhejiang 314000, China.
authors.forename: Yijuan
authors.identifier: 0000-0002-8594-2720
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Hainan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Cao
authors.affiliation: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Sun
authors.affiliation: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Yanyong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Bailong
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Cai
authors.affiliation: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Fu
authors.initials: F
authors.lastname: Gao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D056604:Grape Seed Extract / Q000494:pharmacology*; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008807:Mice, Inbred BALB C; D044945:Proanthocyanidins / Q000494:pharmacology*; D011839:Radiation, Ionizing; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D015996:Survival Rate
chemical_list: D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D044945:Proanthocyanidins; D011837:Radiation-Protective Agents; D008315:Malondialdehyde