title: Novel method for the rapid and specific extraction of multiple β2 -agonist residues in food by tailor-made Monolith-MIPs extraction disks and detection by gas chromatography with mass spectrometry.
abstract: A quick and specific pretreatment method based on a series of extraction clean-up disks, consisting of molecularly imprinted polymer monoliths and C18 adsorbent, was developed for the specific enrichment of salbutamol and clenbuterol residues in food. The molecularly imprinted monolithic polymer disk was synthesized using salbutamol as a template through a one-step synthesis process. It can simultaneously and specifically recognize salbutamol and clenbuterol. The monolithic polymer disk and series of C18 disks were assembled with a syringe to form a set of tailor-made devices for the extraction of target molecules. In a single run, salbutamol and clenbuterol can be specifically extracted, cleaned, and eluted by methanol/acetic acid/H2 O. The target molecules, after a silylation derivatization reaction were detected by gas chromatography-mass spectrometry. The parameters including solvent desorption, sample pH, and the cycles of reloading were investigated and discussed. Under the optimized extraction and clean-up conditions, the limits of detection and quantitation were determined as 0.018-0.022 and 0.042-0.049 ng/g for salbutamol and clenbuterol, respectively. The assay described was convenient, rapid, and specific; thereby potentially efficient in the high-throughput analysis of β2 -agonists residues in real food samples.
authors.affiliation: Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Liu
authors.affiliation: Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, China. ganning@nbu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Gan
authors.affiliation: Department of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing, China.
authors.forename: Yinji
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Faculty of Marine Science, Ningbo University, Ningbo, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.affiliation: Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, China.
authors.forename: Saichai
authors.initials: S
authors.lastname: Lin
authors.affiliation: Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, China.
authors.forename: Tianhua
authors.initials: T
authors.lastname: Li
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis / Q000302:isolation & purification*; D000327:Adsorption; D000420:Albuterol / Q000032:analysis / Q000302:isolation & purification*; D000818:Animals; D002976:Clenbuterol / Q000032:analysis / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D008460:Meat / Q000032:analysis*; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013552:Swine
chemical_list: D000318:Adrenergic beta-Agonists; D011108:Polymers; D000420:Albuterol; D002976:Clenbuterol
keywords: Agonists; Foodstuffs; Gas chromatography-mass spectrometry; Molecularly imprinted polymers; Monoliths