title: Transcript Profiling of Toll-Like Receptor mRNAs in Selected Tissues of Mink (Neovison vison).
abstract: Toll-like receptors (TLRs) can recognize conserved molecular patterns and initiate a wide range of innate and adaptive immune responses against invading infectious agents. The aim of this study was to assess the transcript profile of mink TLRs (mTLRs) in mink peripheral blood mononuclear cells (PBMCs) and a range of tissues, and to explore the potential role of mTLRs in the antiviral immune response process. The results indicated that the mTLR partial nucleotide sequences had a high degree of nucleotide identity with ferret sequences (95-98%). Phylogenetic analysis showed that mammalian TLRs grouped into five TLR families, with a closer relationship of the mTLRs with those of ferret than the other mammalian sequences. Moreover, all the mTLRs were ubiquitously expressed in lymphoid organs (spleen and lymph nodes) and PBMCs. Interestingly, the mTLR expression patterns in lung, uterus, and heart showed quite a lot of similarity. Another remarkable observation was the wide expression of mTLR1-3 mRNAs in all tissues. Among the analyzed tissues, skeletal muscle was revealed to being the lowest repertoire of mTLR expression. Additionally, mink PBMCs exposed to the canine distemper virus revealed significant upregulation of mTLR2, mTLR4, mTLR7, and mTLR8 mRNAs, indicating that mTLRs have a role in innate immunity in the mink. Collectively, our results are the first to establish the basic expression patterns of mTLRs and the relationship between mTLRs and a virus, which will contribute to better understanding of the evolution and the functions of mTLRs in the innate immune system in minks.
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, 130122, Jilin Province, P.R. China.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Tong
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, 130122, Jilin Province, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Yi
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, 130122, Jilin Province, P.R. China.
authors.forename: Yuening
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, 130122, Jilin Province, P.R. China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, 130122, Jilin Province, P.R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Cao
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, 130122, Jilin Province, P.R. China.
authors.forename: Jianke
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, 130122, Jilin Province, P.R. China.
authors.forename: Hang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, 130122, Jilin Province, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lin
authors.affiliation: Wu Xi Medical School, Jiangnan University, Wu Xi, 214122, Jiangsu Province, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, 130122, Jilin Province, P.R. China.
authors.forename: Shipeng
authors.initials: S
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D020869:Gene Expression Profiling; D007113:Immunity, Innate; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008168:Lung / Q000276:immunology; D008907:Mink / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000821:virology; D018482:Muscle, Skeletal / Q000276:immunology; D013154:Spleen / Q000276:immunology; D051193:Toll-Like Receptors / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic; D014777:Virus Diseases / Q000235:genetics / Q000276:immunology / Q000662:veterinary* / Q000821:virology
chemical_list: D051193:Toll-Like Receptors
keywords: Neovison vison; Toll-like receptors; innate immunity; transcript profiling