title: Anti-tumor effect of Radix Paeoniae Rubra extract on mice bladder tumors using intravesical therapy.
abstract: Radix Paeoniae Rubra (RPR) is the dried root ofPaeonia lactifloraPallas andPaeonia veitchiiLynch, and is a herbal medicine that is widely used in traditional Chinese medicine for the treatment of blood-heat and blood-stasis syndrome, similarly to Cortex Moutan. The present study identified the same three components in RPR and Cortex Moutan extracts. In addition, it has been reported that RPR has an anti-cancer effect. Bladder cancer is the seventh most common type of cancer worldwide. Due to the high recurrence rate, identifying novel drugs for bladder cancer therapy is essential. In the present study, RPR extract was evaluated as a bladder cancer therapyin vitroandin vivo. The present results revealed that RPR extract reduced the cell viability of bladder cancer cells with a half maximal inhibitory concentration of 1-3 mg/ml, and had an extremely low cytotoxic effect on normal urothelial cells. Additionally, RPR decreased certain cell cycle populations, predominantly cells in the G1 phase, and caused a clear sub-G increase. In a mouse orthotopic bladder tumor model, intravesical application of RPR extract decreased the bladder tumor size without altering the blood biochemical parameters of the mice. In summary, the present results demonstrate the anti-proliferative properties of RPR extract on bladder cancer cells, and its anti-bladder tumor effectin vivo. Compared to Cortex Moutan extract, RPR extract may provide a more effective alternative therapeutic strategy for the intravesical therapy of superficial bladder cancer.
authors.affiliation: Graduate Institute of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan R.O.C.; Department of Chinese Medicine, Chiayi Christian Hospital, College of Life Sciences, National Chiayi University, Chiayi 600, Taiwan R.O.C.
authors.forename: Mei-Yi
authors.initials: MY
authors.lastname: Lin
authors.affiliation: Graduate Institute of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan R.O.C.
authors.forename: Su-Yin
authors.initials: SY
authors.lastname: Chiang
authors.affiliation: Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi 600, Taiwan R.O.C.
authors.forename: Yi-Zhen
authors.initials: YZ
authors.lastname: Li
authors.affiliation: Department of Biochemical Science and Technology, College of Life Sciences, National Chiayi University, Chiayi 600, Taiwan R.O.C.
authors.forename: Mei-Fang
authors.initials: MF
authors.lastname: Chen
authors.affiliation: Graduate Institute of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan R.O.C.; Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung 404, Taiwan R.O.C.
authors.forename: Yueh-Sheng
authors.initials: YS
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi 600, Taiwan R.O.C.
authors.forename: Jin-Yi
authors.initials: JY
authors.lastname: Wu
authors.affiliation: Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi 600, Taiwan R.O.C.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Liu
keywords: Radix Paeoniae Rubra; bladder cancer; gallic acid; intravesical therapy; methyl gallate; mouse orthotopic bladder tumor model; paeoniflorin