title: Tetraphenylporphyrin as a protein label for triple detection analytical systems.
abstract: Porphyrins and metalloporphyrins are promising new protein labels that can be detected using multiple techniques; improving the reliability of the analysis and broadening the range of the linear response. Here, we investigate the potential of 5,10,15,20-tetraphenyl-21H,23H-porphyrin (Tpp) as a hybrid protein label. The electrochemical and optical properties of porphyrin conjugated with bovine serum albumin (BSA), chicken egg albumin (CEA) and immunoglobulin G (IgG) were determined and optimal conditions for Tpp-protein conjugation established. Model conjugates of carboxylated Tpp with BSA and short peptides were characterized using differential pulse voltammetry, UV-Vis spectrophotometry and spectrofluorimetry. These results reveal that Tpp is a promising molecule to be used in a triple detection protein labelling system.
authors.affiliation: Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology Noakowskiego 3, 00-664 Warsaw, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Konopińska
authors.affiliation: Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology Noakowskiego 3, 00-664 Warsaw, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Pietrzak
authors.affiliation: Department of Metabolic Regulation, Institute of Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Mazur
authors.affiliation: Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology Noakowskiego 3, 00-664 Warsaw, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Malinowska
keywords: Analytical chemistry; Biochemistry; Chemistry; Electrochemistry; Protein modification; Proteins; Spectroscopy; Visible spectrophotometry; Voltammetry