title: MicroRNA-145 Inhibitor Significantly Improves the Development of Bovine Somatic Cell Nuclear Transfer Embryos In Vitro.
abstract: Directly regulating the translation of POU5F1, SOX2, KLF4, and miRNA-145 plays an important role in maintaining the pluripotency of stem cells and the development of early embryos. In the present study, the expression model of miRNA-145 on bovine somatic cell nuclear transfer (SCNT) and in vitro fertilized (IVF) embryos were investigated and compared. Results indicated that (1) the expression level of miRNA-145 was significantly higher in SCNT embryos than that in IVF embryos after the eight-cell stage; (2) miRNA-145 negatively regulated the POU5F1, SOX2, and KLF4 in bovine embryos; (3) decreasing the expression of miRNA-145 by the miRNA-145 inhibitor significantly enhanced the expression of these three genes and the blastocyst formation rate; it also increased the total cell number and inner cell mass ratio of the bovine day 7 SCNT embryos. In conclusion, decreasing miRNA-145 expression might be a feasible means to enhance SCNT efficiency in bovines.
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, China .
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Li
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, China .
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, China .
authors.forename: Fengyu
authors.initials: F
authors.lastname: Wang
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, China .
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, China .
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, China .
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, China .
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, China .
authors.forename: Yaping
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002417:Cattle / Q000196:embryology*; D002478:Cells, Cultured; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000235:genetics*; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D055748:SOXB1 Transcription Factors / Q000378:metabolism
chemical_list: D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D035683:MicroRNAs; D050814:Octamer Transcription Factor-3; D055748:SOXB1 Transcription Factors