title: Five and four dimensional experiments for robust backbone resonance assignment of large intrinsically disordered proteins: application to Tau3x protein.
abstract: New experiments dedicated for large IDPs backbone resonance assignment are presented. The most distinctive feature of all described techniques is the employment of MOCCA-XY16 mixing sequences to obtain effective magnetization transfers between carbonyl carbon backbone nuclei. The proposed 4 and 5 dimensional experiments provide a high dispersion of obtained signals making them suitable for use in the case of large IDPs (application to 354 a. a. residues of Tau protein 3x isoform is presented) as well as provide both forward and backward connectivities. What is more, connecting short chains interrupted with proline residues is also possible. All the experiments employ non-uniform sampling.
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, 02093, Warsaw, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Żerko
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, 02093, Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Byrski
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, 02093, Warsaw, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Włodarczyk-Pruszyński
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, 02093, Warsaw, Poland.|Section of Biophysics, Faculty of Physics, University of Warsaw, 02093, Warsaw, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Górka
authors.affiliation: Department of Computational and Structural Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
authors.forename: Karin
authors.initials: K
authors.lastname: Ledolter
authors.affiliation: Departments of Neuroscience and Pathology, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Eliezer
authors.initials: E
authors.lastname: Masliah
authors.affiliation: Department of Computational and Structural Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
authors.forename: Robert
authors.initials: R
authors.lastname: Konrat
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, 02093, Warsaw, Poland. kozmin@chem.uw.edu.pl.
authors.forename: Wiktor
authors.initials: W
authors.lastname: Koźmiński
mesh_terms: D000595:Amino Acid Sequence; D064267:Intrinsically Disordered Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D020033:Protein Isoforms; D051844:alpha-Synuclein / Q000737:chemistry; D016875:tau Proteins / Q000737:chemistry
chemical_list: D064267:Intrinsically Disordered Proteins; D020033:Protein Isoforms; D051844:alpha-Synuclein; D016875:tau Proteins
keywords: High-dimensionality NMR; Intrinsically disordered proteins; Isotropic mixing; MOCCA-XY16; Non-uniform sampling; Resonance assignment