title: Cry1Ab-expressing rice did not influence expression of fecundity-related genes in the wolf spider Pardosa pseudoannulata.
abstract: The impact of Bacillus thuringiensis (Bt) toxin proteins on non-target predatory arthropods is not well understood at the cellular and molecular levels. Here, we investigated the potential effects of Cry1Ab expressing rice on fecundity of the wolf spider, Pardosa pseudoannulata, and some of the underlying molecular mechanisms. The results indicated that brown planthoppers (BPHs) reared on Cry1Ab-expressing rice accumulated the Cry toxin and that reproductive parameters (pre-oviposition period, post-oviposition stage, number of eggs, and egg hatching rate) of the spiders that consumed BPHs reared on Bt rice were not different from those that consumed BPHs reared on the non-Bt control rice. The accumulated Cry1Ab did not influence several vitellin (Vt) parameters, including stored energy and amino acid composition, during one generation. We considered the possibility that the Cry toxins exert their influence on beneficial predators via more subtle effects detectable at the molecular level in terms of gene expression. This led us to transcriptome analysis to detect differentially expressed genes in the ovaries of spiders exposed to dietary Cry1Ab and their counterpart control spiders. Eight genes, associated with vitellogenesis, vitellogenin receptor activity, and vitellin membrane formation were not differentially expressed between ovaries from the treated and control spiders, confirmed by qPCR analysis. We infer that dietary Cry1Ab expressing rice does not influence fecundity, nor expression levels of Vt-associated genes in P. pseudoannulata.
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha, Hunan 410128, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China.
authors.forename: Yuan-De
authors.initials: YD
authors.lastname: Peng
authors.affiliation: Longping College, Central South University, Changsha, Hunan 410001,China.
authors.forename: Chao
authors.initials: C
authors.lastname: He
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha, Hunan 410128, China.
authors.forename: Bao-Yang
authors.initials: BY
authors.lastname: Wei
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha, Hunan 410128, China.
authors.forename: Yun-Shan
authors.initials: YS
authors.lastname: Liang
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha, Hunan 410128, China.
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Yang
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha, Hunan 410128, China. Electronic address: wangzhisipder@hotmail.com.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: USDA-ARS, Biological Control of Insects Research Laboratory, Columbia, MO 65203, USA.
authors.forename: David
authors.initials: D
authors.lastname: Stanley
authors.affiliation: Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA. Electronic address: SongQ@missouri.edu.
authors.forename: Qi-Sheng
authors.initials: QS
authors.lastname: Song
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005298:Fertility / Q000235:genetics*; D019476:Insect Proteins; D008297:Male; D012275:Oryza / Q000235:genetics* / Q000469:parasitology; D010053:Ovary / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D013112:Spiders / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D059467:Transcriptome; D019076:Transgenes; D047048:Vitellins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C095416:Cry toxin receptors; D019476:Insect Proteins; D011956:Receptors, Cell Surface; D047048:Vitellins
keywords: Cry1Ab protein; P. pseudoannulata; Transcriptome; Vitellin