title: Feasibility of Human Amniotic Fluid Derived Stem Cells in Alleviation of Neuropathic Pain in Chronic Constrictive Injury Nerve Model.
abstract: OBJECTIVE
  The neurobehavior of neuropathic pain by chronic constriction injury (CCI) of sciatic nerve is very similar to that in humans, and it is accompanied by a profound local inflammation response. In this study, we assess the potentiality of human amniotic fluid derived mesenchymal stem cells (hAFMSCs) for alleviating the neuropathic pain in a chronic constriction nerve injury model.
  
  
  METHODS
  This neuropathic pain animal model was conducted by four 3-0 chromic gut ligatures loosely ligated around the left sciatic nerve in Sprague-Dawley rats. The intravenous administration of hAFMSCs with 5x105 cells was conducted for three consecutive days.
  
  
  RESULTS
  The expression IL-1β, TNF-α and synaptophysin in dorsal root ganglion cell culture was remarkably attenuated when co-cultured with hAFMSCs. The significant decrease of PGP 9.5 in the skin after CCI was restored by administration of hAFMSCs. Remarkably increased expression of CD 68 and TNF-α and decreased S-100 and neurofilament expression in injured nerve were rescued by hAFMSCs administration. Increases in synaptophysin and TNF-α over the dorsal root ganglion were attenuated by hAFMSCs. Significant expression of TNF-α and OX-42 over the dorsal spinal cord was substantially attenuated by hAFMSCs. The increased amplitude of sensory evoked potential as well as expression of synaptophysin and TNF-α expression was alleviated by hAFMSCs. Human AFMSCs significantly improved the threshold of mechanical allodynia and thermal hyperalgesia as well as various parameters of CatWalk XT gait analysis.
  
  
  CONCLUSIONS
  Human AFMSCs administration could alleviate the neuropathic pain demonstrated in histomorphological alteration and neurobehavior possibly through the modulation of the inflammatory response.
authors.affiliation: Institute of Biomedical Sciences, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Chien-Yi
authors.initials: CY
authors.lastname: Chiang
authors.affiliation: Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Shih-An
authors.initials: SA
authors.lastname: Liu
authors.affiliation: Institute of Biomedical Sciences, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Meei-Ling
authors.initials: ML
authors.lastname: Sheu
authors.affiliation: Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: Fu-Chou
authors.initials: FC
authors.lastname: Chen
authors.affiliation: Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Chen
authors.affiliation: Institute of Life Sciences, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Su
authors.affiliation: Department of Neurosurgery, Taichung Veterans General Hospital, Taichung, Taiwan.|Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Hung-Chuan
authors.initials: HC
authors.lastname: Pan
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D000818:Animals; D001522:Behavior, Animal; D002478:Cells, Cultured; D002908:Chronic Disease; D003250:Constriction; D004195:Disease Models, Animal; D005073:Evoked Potentials, Somatosensory; D005240:Feasibility Studies; D005684:Gait; D006801:Humans; D006930:Hyperalgesia / Q000150:complications / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D007249:Inflammation / Q000150:complications / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism; D009416:Nerve Regeneration; D009437:Neuralgia / Q000150:complications / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000473:pathology* / Q000503:physiopathology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D016708:Synaptophysin / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D016708:Synaptophysin; D014409:Tumor Necrosis Factor-alpha; C489034:UCHL1 protein, rat; D043222:Ubiquitin Thiolesterase