title: Construction of a tunable multi-enzyme-coordinate expression system for biosynthesis of chiral drug intermediates.
abstract: Systems that can regulate and coordinate the expression of multiple enzymes for metabolic regulation and synthesis of important drug intermediates are poorly explored. In this work, a strategy for constructing a tunable multi-enzyme-coordinate expression system for biosynthesis of chiral drug intermediates was developed and evaluated by connecting protein-protein expressions, regulating the strength of ribosome binding sites (RBS) and detecting the system capacity for producing chiral amino acid. Results demonstrated that the dual-enzyme system had good enantioselectivity, low cost, high stability, high conversion rate and approximately 100% substrate conversion. This study has paved a new way of exploring metabolic mechanism of functional genes and engineering whole cell-catalysts for synthesis of chiral α-hydroxy acids or chiral amino acids.
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.|The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.|The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China.|The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian, 361005, China.
authors.forename: Baishan
authors.initials: B
authors.lastname: Fang
mesh_terms: D055162:Biocatalysis; D004591:Electrophoresis, Polyacrylamide Gel; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000138:chemical synthesis*
chemical_list: D004798:Enzymes; D004364:Pharmaceutical Preparations; D007930:Leucine