title: Protective effect of grape seed and skin extract against diabetes-induced oxidative stress and renal dysfunction in virgin and pregnant rat.
abstract: The present work deal with the effect of alloxan-induced diabetes on kidney oxidative stress and dysfunction of virgin and pregnant rat as well as the protection that may be afforded by high dosage GSSE (4g/kg) treatment. Diabetes affected negatively several kidney function parameters as creatinemia, uremia, uricemia and proteinuria without affecting kidney index. Diabetes also induced an oxidative stress characterized by increased lipid and protein oxidation, a drop in antioxidant enzyme defenses as catalase, superoxide-dismutase, glutathione-peroxidase, an alteration in transition metals as free iron, copper, selenium and associated enzymes and an increase in calpain and acetyl-cholinesterase activities. Tremendously, GSSE treatment protected efficiently against all the deleterious effects of diabetes-induced kidney dysfunction in both virgin and pregnant animals. High dosage GSSE is a safe and potent anti-oxidant that may be further tested in clinical trials for the long-term preservation of kidney function especially in multiple pregnancies.
authors.affiliation: University of Carthage, Faculty of Sciences of Bizerte, 7021 Jarzouna, Tunisia; Laboratory of Bioactives Substances, Center of Biotechnology of Borj Cedria, BP 901, 2050 Hammam Lif, Tunisia.
authors.forename: Nourhene
authors.initials: N
authors.lastname: Oueslati
authors.affiliation: University of Carthage, Faculty of Sciences of Bizerte, 7021 Jarzouna, Tunisia; Laboratory of Bioactives Substances, Center of Biotechnology of Borj Cedria, BP 901, 2050 Hammam Lif, Tunisia. Electronic address: charradi3@yahoo.com.
authors.forename: Kamel
authors.initials: K
authors.lastname: Charradi
authors.affiliation: University of Carthage, Faculty of Sciences of Bizerte, 7021 Jarzouna, Tunisia; Laboratory of Bioactives Substances, Center of Biotechnology of Borj Cedria, BP 901, 2050 Hammam Lif, Tunisia.
authors.forename: Takwa
authors.initials: T
authors.lastname: Bedhiafi
authors.affiliation: Laboratory of Bioactives Substances, Center of Biotechnology of Borj Cedria, BP 901, 2050 Hammam Lif, Tunisia.
authors.forename: Ferid
authors.initials: F
authors.lastname: Limam
authors.affiliation: University of Carthage, Faculty of Sciences of Bizerte, 7021 Jarzouna, Tunisia; Laboratory of Bioactives Substances, Center of Biotechnology of Borj Cedria, BP 901, 2050 Hammam Lif, Tunisia.
authors.forename: Ezzedine
authors.initials: E
authors.lastname: Aouani
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002853:Chromatography, Liquid; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D042541:Intracellular Space / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008670:Metals / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011247:Pregnancy; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D050050:Protein Carbonylation / Q000187:drug effects; D012639:Seeds / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D008670:Metals; D010636:Phenols; D010936:Plant Extracts; D020011:Protective Agents
keywords: Diabetes; Grape seed and skin extract; Kidney protection; Oxidative stress; Rat