title: Roles of endoplasmic reticulum stress, apoptosis and autophagy in 2,2',4,4'-tetrabromodiphenyl ether-induced rat ovarian injury.
abstract: Endocrine disruptor 2,2',4,4'-tetrabromodiphenylether (PBDE-47) can harm the female reproductive system. Recent studies showed that PBDE-47 neurotoxicity is associated with endoplasmic reticulum stress (ERS); however, the role of ERS in PBDE-47-induced ovarian injury is unclear. New-born female Sprague-Dawley rats were orally exposed to PBDE-47 (1, 5, or 10mg/kg bw) on postnatal day 10. An additional 10mg/kg bw PBDE-47 group was given the ERS inhibitor 4-PBA intraperitoneally for three weeks beginning on postnatal day 8. At 2 months of age, PBDE-47 exposure significantly reduced the ovarian coefficients, increased the expression of ERS and autophagy markers, including GRP78, IRE1, Caspase-12, Beclin1, LC3 and P62. In the 10mg/kg bw PBDE-47 group, PARP and Caspase-3 were markedly activated, indicative of apoptosis. These were accompanied by histopathological damage. Intriguingly, 4-PBA attenuated all these effects. Thus, these results suggest that ERS plays a vital role in PBDE-47-induced ovarian injury by regulating autophagy and apoptosis.
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Shun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Rulin
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Bei
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Niu
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xia
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Pei
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China. Electronic address: wangaiguo@mails.tjmu.edu.cn.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects*; D002082:Butylamines / Q000494:pharmacology; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D005260:Female; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D010053:Ovary / Q000187:drug effects* / Q000473:pathology; D020011:Protective Agents / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley
chemical_list: C121358:4-phenylbutylamine; D002082:Butylamines; D055768:Halogenated Diphenyl Ethers; D020011:Protective Agents; C511295:2,2',4,4'-tetrabromodiphenyl ether
keywords: 2,2′,4,4′-Tetrabromodiphenyl ether; Apoptosis; Autophagy; Endoplasmic reticulum stress; Ovary