title: Antioxidant compounds and their bioaccessibility in tomato fruit and puree obtained from a DETIOLATED-1 (DET-1) down-regulated genetically modified genotype.
abstract: The economic value, the ease of cultivation and processing, and the well-known health-promoting properties of tomato fruit, make the tomato an important target for genetic manipulation to increase its nutritional content. A transgenic variety, down-regulated in the DETIOLATED-1 (DET-1) gene, has been studied in comparison with the parental line, for antioxidant levels in fresh and hot break fruit, as well as the bioaccessibility of antioxidants from puree. Differences in the concentrations of antioxidants between the wild-type and the genetically modified raw tomatoes were confirmed, but antioxidant levels were maintained to a greater extent in the GM puree than in the parent. The bioaccessibility of the compounds, tested using an in vitro digestion model, showed an increase in the genetically modified samples.
authors.affiliation: Departamento de Tecnología de Alimentos, Universitat Politècnica de València, Camino de Vera, s/n 46022, Valencia, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Talens
authors.affiliation: Centre for Systems and Synthetic Biology, School of Biological Sciences, Royal Holloway University of London, Egham Hill, Egham, Surrey TW20 OEX, UK.
authors.forename: L
authors.initials: L
authors.lastname: Mora
authors.affiliation: Centre for Systems and Synthetic Biology, School of Biological Sciences, Royal Holloway University of London, Egham Hill, Egham, Surrey TW20 OEX, UK.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Bramley
authors.affiliation: Centre for Systems and Synthetic Biology, School of Biological Sciences, Royal Holloway University of London, Egham Hill, Egham, Surrey TW20 OEX, UK. Electronic address: p.fraser@royalholloway.ac.uk.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Fraser
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D015536:Down-Regulation; D005638:Fruit / Q000737:chemistry*; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry
chemical_list: D000975:Antioxidants; D010940:Plant Proteins
keywords: Antioxidants; Bioaccessibility; Genetically modified tomato; Thermal processing; Tomato puree