title: Numerical and experimental study of capillary-driven flow of PCR solution in hybrid hydrophobic microfluidic networks.
abstract: Capillary-driven microfluidics is essential for development of point-of-care diagnostic micro-devices. Polymerase chain reaction (PCR)-based micro-devices are widely developed and used in such point-of-care settings. It is imperative to characterize the fluid parameters of PCR solution for designing efficient capillary-driven microfluidic networks. Generally, for numeric modelling, the fluid parameters of PCR solution are approximated to that of water. This procedure leads to inaccurate results, which are discrepant to experimental data. This paper describes mathematical modeling and experimental validation of capillary-driven flow inside Poly-(dimethyl) siloxane (PDMS)-glass hybrid micro-channels. Using experimentally measured PCR fluid parameters, the capillary meniscus displacement in PDMS-glass microfluidic ladder network is simulated using computational fluid dynamic (CFD), and experimentally verified to match with the simulated data.
authors.affiliation: Fluidigm Corporation, 7000 Shoreline Court, South San Francisco, CA, 94080, USA. naveensma@gmail.com.|School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore, 639798, Singapore. naveensma@gmail.com.
authors.forename: Naveen
authors.initials: N
authors.lastname: Ramalingam
authors.affiliation: School of Mechanical and Manufacturing Engineering, Australian Center for NanoMedicine, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Majid Ebrahimi
authors.initials: ME
authors.lastname: Warkiani
authors.affiliation: Department of Electrical and Computer Engineering, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Neevan
authors.initials: N
authors.lastname: Ramalingam
authors.affiliation: School of Mechanical and Manufacturing Engineering, Australian Center for NanoMedicine, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Keshavarzi
authors.affiliation: School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore, 639798, Singapore.|Wisewater Pte Ltd., 71 Ayer Rajah Crescent, Singapore, 139951, Singapore.
authors.forename: Liu
authors.initials: L
authors.lastname: Hao-Bing
authors.affiliation: School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore, 639798, Singapore. mhqgong@ntu.edu.sg.
authors.forename: Thomas Gong
authors.initials: TG
authors.lastname: Hai-Qing
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics; D008962:Models, Theoretical; D009757:Nylons / Q000737:chemistry; D017830:Octoxynol / Q000737:chemistry; D019095:Point-of-Care Systems; D016133:Polymerase Chain Reaction; D012996:Solutions; D013499:Surface Properties
chemical_list: D004129:Dimethylpolysiloxanes; D009757:Nylons; D012996:Solutions; C085707:poly(dimethylsiloxane)-polyamide copolymer; D017830:Octoxynol
keywords: Capillary; Microfluidics; Polymerase chain reaction (PCR); Simulation; Surface hydrophobicity