title: Capillary electrophoresis and phenylboronic acid solid phase extraction for the determination of S-adenosylmethionine/S-adenosylhomocysteine ratio in human urine.
abstract: An approach that allows direct analysis of the ratio of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) by using CE is presented. The analytes were extracted on phenylboronic acid phase and eluted with 100 mmol/L HCl. CE separation of the analytes took place in the transient isotachophoresis mode with addition of NaCl and meglumine to the samples. The sensitivity (S/N = 3) and quantification limit (S/N = 10) of the method were 0.07 and 0.2 μmol/L, respectively, using a silica capillary with 50 μm internal diameter and 30.5 cm total length. The BGE was 0.02 mol/L Tris with 1 mol/L HCOOH (pH 2.2), and the separation voltage was 15-17 kV. Accuracy of SAM and SAH analysis in urine was 96 and 105%, respectively; interday precision for the SAM/SAH ratio was within 6%. The theoretical plate number exceeded a million. Total analysis time was 8.5 min.
authors.affiliation: Federal State Budgetary Scientific Institution "Institute of General Pathology and Pathophysiology,", Moscow, Russia. Ivanov_av82@mail.ru.
authors.forename: Alexander Vladimirovich
authors.initials: AV
authors.lastname: Ivanov
authors.affiliation: Federal State Budgetary Scientific Institution "Institute of General Pathology and Pathophysiology,", Moscow, Russia.
authors.forename: Edward Danielevich
authors.initials: ED
authors.lastname: Virus
authors.affiliation: Federal State Budgetary Scientific Institution "Institute of General Pathology and Pathophysiology,", Moscow, Russia.
authors.forename: Ksenya Alexandrovna
authors.initials: KA
authors.lastname: Nikiforova
authors.affiliation: N. N. Blokhin Russian Cancer Research Center, Moscow, Russia.
authors.forename: Nicolai Evgenevich
authors.initials: NE
authors.lastname: Kushlinskii
authors.affiliation: Federal State Budgetary Scientific Institution "Institute of General Pathology and Pathophysiology,", Moscow, Russia.
authors.forename: Boris Petrovich
authors.initials: BP
authors.lastname: Luzyanin
authors.affiliation: Research Center of Neurology, Moscow, Russia.
authors.forename: Marina Yurievna
authors.initials: MY
authors.lastname: Maksimova
authors.affiliation: Research Center of Neurology, Moscow, Russia.
authors.forename: Mikhail Aleksanrovich
authors.initials: MA
authors.lastname: Piradov
authors.affiliation: Federal State Budgetary Scientific Institution "Institute of General Pathology and Pathophysiology,", Moscow, Russia.
authors.forename: Aslan Amirkhanovich
authors.initials: AA
authors.lastname: Kubatiev
mesh_terms: D000328:Adult; D000368:Aged; D001897:Boronic Acids / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012435:S-Adenosylhomocysteine / Q000652:urine*; D012436:S-Adenosylmethionine / Q000652:urine*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D055815:Young Adult
chemical_list: D001897:Boronic Acids; D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine; C010686:benzeneboronic acid
keywords: Capillary electrophoresis; S-adenosylhomocysteine; S-adenosylmethionine; Urine