title: Effects of ganoderic acids on epileptiform discharge hippocampal neurons: insights from alterations of BDNF,TRPC3 and apoptosis.
abstract: Recently, Ganoderma lucidum spores (GLS) have shown anti-epileptic effects. However, there are no reports on the anti-epileptic effects of its chemical constituents ganoderic acids (GAs), and more research is needed to better understand the mechanism of GLS activity. In this work, rat primary hippocampal neurons in an in vitro model were used to assess the intervention effects of GAs on epileptiform discharge hippocampal neurons and expression of both BDNF and TRPC3, with the aid of immunofluorescence, MTT method and flow cytometry. It was found that BDNF and TRPC3 are expressed in all cells and were mainly localized in the cytoplasm. The fluorescence intensities of BDNF and TRPC3 in GAs groups were higher than those of normal control and model groups, especially at 80 μg/ml (P < 0.05). The apoptosis rate of neurons was inversely proportional to BDNF and TRPC3 changes (P < 0.01). Therefore, BDNF and TRPC3 should be involved in the occurrence and development of epilepsy. GAs might indirectly inhibit mossy fiber sprouting and adjust the synaptic reconstructions by promoting the expression of BDNF and TRPC3. Besides, GAs could exert a protective effect on hippocampal neurons by promoting neuronal survival and the recovery of injured neurons.
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Yang
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D003593:Cytoplasm / Q000378:metabolism; D004827:Epilepsy / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D019599:Mossy Fibers, Hippocampal / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar; D020940:Reishi / Q000737:chemistry; D050052:TRPC Cation Channels / Q000096:biosynthesis* / Q000235:genetics; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D000927:Anticonvulsants; D019208:Brain-Derived Neurotrophic Factor; D050052:TRPC Cation Channels; C104603:TRPC3 cation channel; D014315:Triterpenes; C556862:ganoderic acid