title: Role of CD133+cells in tongue squamous carcinomas: Characteristics of 'stemness'in vivoandin vitro.
abstract: The objective of the present study was to determine the 'stemness' characteristics of CD133+cells (harvested from the squamous cell tongue carcinoma Tca-8113 cell line)in vitroand to observe the tumourigenicity of the CD133+cells in the bodies of NOD/SCID mice. Single cells from the Tca-8113 cell line were observed for multiplication capacityin vitro. The suspending and pelletizing phenomena of Tca-8113 cellsin vitrowere also observed, and the expression of CD133 in squamous cell carcinoma of the tongue was measured. The CD133+cells from the Tca-8113 cell line were purified, and their multiplication capacity and differentiation potency were observed. The NOD/SCID mouse model was established, and the tumourigenicity of the CD133+cells was determined. The Tca-8113 cells were observed to emerge in the form of suspending tumour spheres in squamous cell carcinoma of the tongue. Monoplasts with sustainable multiplication capacity accounted for ~5.32% of the spheres, and 0.95% of the CD133+cells were expressed in squamous cell carcinoma of the tongue, with stronger multiplication capacity and differentiation potencyin vitro. Stronger tumourigenicity was also observed in the bodies of the NOD/SCID mice. CD133-cells exhibited a multiplication capacity to a certain extent. Overall, the CD133+cells in squamous cell carcinoma of the tongue are characterised by relatively strong tumourigenicity capacityin vivoandin vitro. To a certain extent, these CD133+cells demonstrate the characteristics of 'stemness'.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China; Department of Oral Stomatology, Gongli Hospital, The Second Military Medical University, Shanghai 200135, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China.
authors.forename: Xiao-Kang
authors.initials: XK
authors.lastname: Zhou
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China.
authors.forename: Fei-Wu
authors.initials: FW
authors.lastname: Kang
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China.
authors.forename: Zuo-Lin
authors.initials: ZL
authors.lastname: Wang
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
keywords: CD133+ cell; NOD/SCID mice; tongue squamous carcinoma; tumour stem cell; tumourigenicity