title: Restore a 9 mm diameter osteochondral defect with gene enhanced tissue engineering followed mosaicplasty in a goat model.
abstract: OBJECTIVE
  The aim of this study was to evaluate the efficacy of gene enhanced tissue engineering followed mosaicplasty in a goat model.
  
  
  METHODS
  An acute cylindrical defect 9 mm in diameter was created in the weight bearing area of the medial femoral condyle in a goat model. Thirty-six medial femoral condyles were divided into 6 groups using different proportion of gene enhanced tissue engineering and mosaicplasty to restore the defects. The specimen received gross and histology observation, which was evaluated by the histological grading scale of O'Driscoll, Keeley and Salter. Transmission electron microscope observation was also performed. Two factors analysis of variance and Student-Newman-Kewls test were used to compare the specimen.
  
  
  RESULTS
  The gross and histology observation revealed that each defects of six groups had different restoration. The scores of the reparative tissue of three groups with gene enhancement were significantly higher than those in other three groups without gene enhancement (p > 0.05).
  
  
  CONCLUSIONS
  Gene enhanced tissue engineering followed mosaicplasty could restore a 9 mm diameter osteochondral defects in a goat model effectively. With the reduction of covering area of the graft, the advantages of the combined gene enhanced tissue engineering method can be better reflected.
authors.affiliation: Department of Orthopedics, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200021, China. Electronic address: drsunjun@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Orthopedics, No. 9 Hospital Affiliated to Shanghai Jiaotong University, China. Electronic address: mengshi897euie3@163.com.
authors.forename: Xiao-Kui
authors.initials: XK
authors.lastname: Hou
authors.affiliation: Department of Orthopedics, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200021, China. Electronic address: prozrm1@126.com.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000473:pathology*; D005269:Femur / Q000601:surgery*; D006041:Goats; D007719:Knee Joint / Q000601:surgery*; D045164:Mesenchymal Stem Cell Transplantation; D023822:Tissue Engineering / Q000379:methods*; D014161:Transduction, Genetic
keywords: Bone mesenchymal stem cells (BMSCs); Gene transduction; Osteochondral repair; Tissue engineering