title: MicroRNA-328a regulates water maze performance in PTZ-kindled rats.
abstract: The down-regulation of microRNA-328a (miR-328a) in pentylenetetrazole (PTZ)-kindled rats with memory impairment was demonstrated in our previous study, while any contribution of miR-328a to cognitive dysfunction of PTZ-kindled rats remains unknown. In this study we have investigated the effect and the underlying mechanism of miR-328a on the cognitive function in PTZ-kindled rats. 48 SD male rats were divided into 4 groups as follows: a PTZ kindled group, a miR-328a antagomir group, an antagomir-control group, and a sham group (n=12 for each). All rats except those from the sham group were treated with PTZ 14 times at intervals of 48h to establish the temporal lobe epilepsy (TLE) models, and miR-328a antagomir was given to the antagomir group as a treatment by lateral-ventricle injection the day after the first injection of PTZ. Morris water maze (MWM) test was performed to assay their learning and memory abilities. The down-regulation of miR-328a in the PTZ group was confirmed using RT-qPCR and the expression of miR-328a was diminished after antagomir treatment (P<0.05). In the probe test of water maze, the time and distance of the PTZ group were both shorter than those of the sham group (P<0.05), and those of the antagomir-control group were both longer than those of the antagomir group (P<0.05). In addition, we found that with the down-regulation of miR-328a, the levels of Beta-site APP-cleaving enzyme (BACE), which is a bioinformatics-predicted target of miR-328a, were up-regulated. These findings suggest that miR-328a may play a role in memory dysfunction in PTZ-kindled rats by regulating the BACE levels and this links the PTZ model with Alzheimer's disease.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Xixia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Luo
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Donghua
authors.initials: D
authors.lastname: Zou
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China; Guangxi Collaborative Innovation Center for Biomedicine, Guangxi Medical University, Nanning 530021, China. Electronic address: wuyuan90@126.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wu
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000378:metabolism; D000818:Animals; D000070416:Antagomirs / Q000494:pharmacology; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects*; D004833:Epilepsy, Temporal Lobe / Q000139:chemically induced*; D019204:GTP-Binding Proteins / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000378:metabolism; D007696:Kindling, Neurologic / Q000187:drug effects* / Q000502:physiology; D008297:Male; D018782:Maze Learning / Q000187:drug effects* / Q000502:physiology; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism; D010433:Pentylenetetrazole / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000070416:Antagomirs; C501227:Bcl9l protein, rat; D020690:GTPase-Activating Proteins; D035683:MicroRNAs; D009363:Neoplasm Proteins; C112202:Syngap1 protein, rat; D053829:Amyloid Precursor Protein Secretases; D016282:Aspartic Acid Endopeptidases; C509281:Bace1 protein, rat; D019204:GTP-Binding Proteins; D010433:Pentylenetetrazole
keywords: Alzheimer's disease; BACE; Cognitive function; Epilepsy; microRNA-328a