title: Inhibitor degradation and lipid accumulation potentials of oleaginous yeast Trichosporon cutaneum using lignocellulose feedstock.
abstract: Oleaginous yeast Trichosporon cutaneum is robust to high levels of lignocellulose derived inhibitor compounds with considerable lipid accumulation capacity. The potential of lipid accumulation of T. cutaneum ACCC 20271 was investigated using corn stover hydrolysates with varying sugar and inhibitor concentrations. Biodiesel was synthesized using the extracted lipid and the product satisfied the ASTM standards. Among the typical inhibitors, T. cutaneum ACCC 20271 is relatively sensitive to furfural and 4-hydroxybenzaldehyde, but strongly tolerant to high titers of formic acid, acetic acid, levulinic acid, HMF, vanillin, and syringaldehyde. It is capable of complete degradation of formic acid, acetic acid, vanillin and 4-hydroxybenzaldehyde. Finally, the inhibitor degradation pathways of T. cutaneum ACCC 20271 were constructed based on the newly sequenced whole genome information and the experimental results. The study provided the first insight to the inhibitor degradation of T. cutaneum and demonstrated the potentials of lipid production from lignocellulose.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Qiuqiang
authors.initials: Q
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Huizhan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address: jbao@ecust.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Bao
mesh_terms: D056804:Biofuels; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D014250:Trichosporon / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry
chemical_list: D056804:Biofuels; D008055:Lipids; C036909:lignocellulose; D008031:Lignin
keywords: Biodiesel; Inhibitor degradation; Lignocellulose; Lipid; Trichosporon cutaneum ACCC 20271