title: LOC-SERS: A Promising Closed System for the Identification of Mycobacteria.
abstract: A closed droplet based lab-on-a-chip (LOC) device has been developed for the differentiation of six species of mycobacteria, i.e., both Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM), using surface-enhanced Raman spectroscopy (SERS). The combination of a fast and simple bead-beating module for the disruption of the bacterial cell with the LOC-SERS device enables the application of an easy and reliable system for bacteria discrimination. Without extraction or further treatment of the sample, the obtained SERS spectra are dominated by the cell-wall component mycolic acid. For the differentiation, a robust data set was recorded using a droplet based LOC-SERS device. Thus, more than 2100 individual SERS spectra of the bacteria suspension were obtained in 1 h. The differentiation of bacteria using LOC-SERS provides helpful information for physicians to define the conditions for the treatment of individual patients.
authors.affiliation: Leibniz Institute of Photonic Technology (IPHT) , Albert-Einstein-Strasse 9, 07745 Jena, Germany.|Institute for Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena , Helmholtzweg 4, 07743 Jena, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Mühlig
authors.affiliation: Institute for Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena , Helmholtzweg 4, 07743 Jena, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bocklitz
authors.affiliation: Alere Technologies GmbH, Löbstedter Strasse 103-105, 07743 Jena, Germany.
authors.forename: Ines
authors.initials: I
authors.lastname: Labugger
authors.affiliation: Alere Technologies GmbH, Löbstedter Strasse 103-105, 07743 Jena, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Dees
authors.affiliation: Alere Technologies GmbH, Löbstedter Strasse 103-105, 07743 Jena, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Henk
authors.affiliation: MVZ Laboratory Dr. Limbach & Kollegen GbR, Im Breitspiel 15, 69126 Heidelberg, Germany.
authors.forename: Elvira
authors.initials: E
authors.lastname: Richter
authors.forename: Sönke
authors.initials: S
authors.lastname: Andres
authors.forename: Matthias
authors.initials: M
authors.lastname: Merker
authors.affiliation: Institute for Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena , Helmholtzweg 4, 07743 Jena, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Stöckel
authors.affiliation: Leibniz Institute of Photonic Technology (IPHT) , Albert-Einstein-Strasse 9, 07745 Jena, Germany.|Institute for Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena , Helmholtzweg 4, 07743 Jena, Germany.
authors.forename: Karina
authors.initials: K
authors.lastname: Weber
authors.affiliation: Leibniz Institute of Photonic Technology (IPHT) , Albert-Einstein-Strasse 9, 07745 Jena, Germany.|Institute for Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena , Helmholtzweg 4, 07743 Jena, Germany.
authors.forename: Dana
authors.initials: D
authors.lastname: Cialla-May
authors.affiliation: Leibniz Institute of Photonic Technology (IPHT) , Albert-Einstein-Strasse 9, 07745 Jena, Germany.|Institute for Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena , Helmholtzweg 4, 07743 Jena, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D056656:Lab-On-A-Chip Devices; D009161:Mycobacterium / Q000166:cytology / Q000302:isolation & purification*; D013045:Species Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation; D013499:Surface Properties