title: Ethylene Response Factor TERF1, Regulated by ETHYLENE-INSENSITIVE3-like Factors, Functions in Reactive Oxygen Species (ROS) Scavenging in Tobacco (Nicotiana tabacum L.).
abstract: The phytohormone ethylene plays a crucial role in the production and accumulation of reactive oxygen species (ROS) in plants under stress conditions. Ethylene response factors (ERFs) are important ethylene-signaling regulators functioning in plant defense responses against biotic and abiotic stresses. However, the roles of ERFs during plant adapting to ROS stress have not yet been well documented. Our studies previously reported that a tomato ERF transcription factor TERF1 functions in the regulation of plant ethylene responses and stress tolerance. Here, we report our findings regarding the roles of TERF1 in ROS scavenging. In this study, we revealed that the transcription of TERF1 is regulated by upstream EIN3-like (EIN3, ethylene-insensitive 3) regulators LeEIL3 and LeEIL4 in tomato (Solanum lycopersicum), and is also inducible by exogenous applied ROS-generating reagents. Ectopic expression of TERF1 in tobacco promoted the expression of genes involved in oxidative stress responses, including carbonic anhydrase functioning in hypersensitive defense, catalase and glutathione peroxidase catalyzing oxidative reactions, and GDP-D-mannose pyrophosphorylase functioning in ascorbic acid biosynthesis, reduced the ROS content induced by ethylene treatment, and enhanced stress tolerance of tobacco seedlings to hydrogen peroxide (H2O2). Cumulatively, these findings suggest that TERF1 is an ethylene inducible factor regulating ROS scavenging during stress responses.
authors.affiliation: Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.|Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Zhijin
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Pingli
authors.initials: P
authors.lastname: Lu
authors.affiliation: Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
authors.forename: Dingyu
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
authors.forename: Xinmin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
authors.forename: Zhong-Feng
authors.initials: ZF
authors.lastname: Zhang
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Huang
mesh_terms: D005030:Ethylenes / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D018551:Solanum lycopersicum / Q000378:metabolism*; D010084:Oxidation-Reduction; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D005030:Ethylenes; D016166:Free Radical Scavengers; D010940:Plant Proteins; D017382:Reactive Oxygen Species; C036216:ethylene; D006861:Hydrogen Peroxide