title: The design and fabrication of a three-dimensional bioengineered open ventricle.
abstract: Current treatments in hypoplastic left heart syndrome (HLHS) include multiple surgeries to refunctionalize the right ventricle and/or transplant. The development of a tissue-engineered left ventricle (LV) would provide a therapeutic option to overcome the inefficiencies and limitations associated with current treatment options. This study provides a foundation for the development and fabrication of the bioengineered open ventricle (BEOV) model. BEOV molds were developed to emulate the human LV geometry; molds were used to produce chitosan scaffolds. BEOV were fabricated by culturing 30 million rat neonatal cardiac cells on the chitosan scaffold. The model demonstrated 57% cell retention following 4days culture. The average biopotential output for the model was 1615 µV. Histological assessment displayed the presence of localized cell clusters, with intercellular and cell-scaffold interactions. The BEOV provides a novel foundation for the development of a 3D bioengineered LV for application in HLHS. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2206-2217, 2017.
authors.affiliation: Department of Biomedical Engineering, University of Houston, Houston, Texas.
authors.forename: Nikita M
authors.initials: NM
authors.lastname: Patel
authors.affiliation: Department of Biomedical Engineering, University of Houston, Houston, Texas.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Mohamed
authors.affiliation: Department of Biomedical Engineering, University of Houston, Houston, Texas.|Center for Biomimetic Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.|Department of Orthopedics, Houston Methodist Hospital, Houston, Texas 77030, USA.
authors.forename: Iman K
authors.initials: IK
authors.lastname: Yazdi
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.|Department of Orthopedics, Houston Methodist Hospital, Houston, Texas 77030, USA.
authors.forename: Ennio
authors.initials: E
authors.lastname: Tasciotti
authors.affiliation: Department of Biomedical Engineering, University of Houston, Houston, Texas.
authors.forename: Ravi K
authors.initials: RK
authors.lastname: Birla
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D006352:Heart Ventricles / Q000166:cytology / Q000378:metabolism; D006801:Humans; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D048271:Chitosan
keywords: biocompatibility; cardiac tissue engineering; chitosan; left ventricle; material characterization