title: Microencapsulation of norfloxacin in chitosan/chitosan oligosaccharides and its application in shrimp culture.
abstract: Norfloxacin chitosan/chitosan oligosaccharide microcapsules (NCCM) were prepared by emulsion-chemical crosslinking method. The characteristics of obtained microcapsules were evaluated by scanning electron microscopy, Fourier transform infrared spectroscopy and release experiments. Cumulative release profile of norfloxacin from the chitosan microcapsules in natural seawater was measured and the controlled release of drugs was at a uniform rate in 48h. The chitosan microcapsules were applied onto the antibacterial study of the shrimp culture in natural seawater. It is observed that the seawater in the NCCM added groups was relatively clear and the biomass of Vibrio increased slowly in contrast to the control and norfloxacin groups. The inhibition rate of Vibrio in norfloxacin groups obvioursly decreased after the 5thday, whereas, it remained high and stable during experiment period in NCCM groups. The results showed that the chitosan microcapsules as release materials have excellent antibacterial effects on Vibrio in the farming of Penaeus vannamei Boone. The controlled release could obviously reduce dosage of antibiotics and delivery times, and effectively improve the utilization rate of norfloxacin drugs for shrimps.
authors.affiliation: Marine College, Shandong University, Weihai 264209, China.
authors.forename: Ziru
authors.initials: Z
authors.lastname: Lian
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Pan
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China. Electronic address: jtwang@ouc.edu.cn.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017756:Aquaculture / Q000379:methods*; D018533:Biomass; D002214:Capsules / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D033364:Decapoda / Q000187:drug effects / Q000254:growth & development*; D004339:Drug Compounding; D009643:Norfloxacin / Q000494:pharmacology*; D009844:Oligosaccharides / Q000737:chemistry*; D012623:Seawater; D017550:Spectroscopy, Fourier Transform Infrared; D014733:Vibrio / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D002214:Capsules; D009844:Oligosaccharides; D048271:Chitosan; D009643:Norfloxacin
keywords: Antibacterial activity; Chitosan microcapsules; Controlled release; Norfloxacin; Shrimp culture