title: Thermo-Responsive Polyplex Micelles with PEG Shells and PNIPAM Layer to Protect DNA Cores for Systemic Gene Therapy.
abstract: Simultaneous achievement of prolonged retention in blood circulation and efficient gene transfection activity in target tissues has always been a major challenge hindering in vivo applications of nonviral gene vectors via systemic administration. The engineered strategies for efficient systemic gene delivery are under wide investigation. These approaches include the thermo-responsive formation of a hydrophobic intermediate layer on PEG-shielded polyplex micelles. Herein, we constructed novel rod-shaped ternary polyplex micelles (TPMs) via complexation between the mixed block copolymers of poly(ethylene glycol)-b-poly{N'-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} (PEG-b-PAsp(DET)) and poly(N-isopropylacrylamide)-b-PAsp(DET) (PNIPAM-b-PAsp(DET)) and plasmid DNA (pDNA) at room temperature (RT), exhibiting distinct temperature-responsive formation of a hydrophobic intermediate layer between PEG shells and pDNA cores through facile temperature increase from RT to body temperature (~37 °C).
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, No. 96, Jinzhai Road, Hefei, Anhui, 230026, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, No. 96, Jinzhai Road, Hefei, Anhui, 230026, China.
authors.forename: Zengshi
authors.initials: Z
authors.lastname: Zha
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, No. 96, Jinzhai Road, Hefei, Anhui, 230026, China. gezs@ustc.edu.cn.
authors.forename: Zhishen
authors.initials: Z
authors.lastname: Ge
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis* / Q000737:chemistry; D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D015316:Genetic Therapy; D057927:Hydrophobic and Hydrophilic Interactions; D008113:Liver Neoplasms / Q000235:genetics / Q000628:therapy*; D008297:Male; D051379:Mice; D008823:Micelles; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D008823:Micelles; C052970:poly-N-isopropylacrylamide; D011092:Polyethylene Glycols; D004247:DNA
keywords: Cancer gene therapy; Nonviral gene delivery system; Polyplex micelle; Prolonged blood circulation; Temperature-responsive