title: Change in peripheral blood lymphocyte count in dogs following adoptive immunotherapy using lymphokine-activated T killer cells combined with palliative tumor resection.
abstract: We evaluated changes in peripheral blood lymphocyte (PBL) count in dogs following adoptive immunotherapy using lymphokine-activated T killer cells (T-LAK) in combination with surgery. Fifteen tumor-bearing dogs treated with T-LAK therapy combined with palliative resection of tumors were enrolled in the present study. T-LAK were generated from autologous peripheral blood mononuclear cells (PBMC) by culture with recombinant human interleukin -2 (rhIL-2) and solid phase anti-canine cluster of differentiation (CD)3 antibody. T-LAK were administrated intravenously at 2-4-week intervals. After the first administration of T-LAK, counts of PBL and T lymphocyte subsets (CD3(+), CD4(+) and CD8(+) cells) increased and the CD4/CD8 ratio decreased, with significant increases in CD8(+) cells (P<0.05). In 8 tumor-bearing dogs that were administered sequential T-LAK, available data on changes in PBL and T lymphocyte phenotypes until the fifth administration were also analyzed. In tumor-bearing dogs administered 5 rounds of T-LAK, CD8(+) cell counts were maintained high until the fifth administration of T-LAK. Moreover, the CD4/CD8 ratio remained low until the fifth administration of T-LAK. These results indicate that T-LAK therapy combined with surgery may increase peripheral blood T lymphocytes, particularly CD8(+) cells, in tumor-bearing dogs.
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-oraikita, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Mie
authors.affiliation: Veterinary Medical Center, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-oraikita, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Terumasa
authors.initials: T
authors.lastname: Shimada
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-oraikita, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Akiyoshi
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-oraikita, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Hayashi
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-oraikita, Izumisano, Osaka, 598-8531, Japan. Electronic address: ohashi@vet.osakafu-u.ac.jp.
authors.forename: Fumihito
authors.initials: F
authors.lastname: Ohashi
mesh_terms: D000818:Animals; D003131:Combined Modality Therapy / Q000662:veterinary; D004285:Dogs; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods / Q000662:veterinary*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D018655:Lymphocyte Count; D008297:Male; D009369:Neoplasms / Q000276:immunology / Q000628:therapy / Q000662:veterinary*; D010166:Palliative Care; D016176:T-Lymphocyte Subsets / Q000276:immunology
keywords: Adoptive immunotherapy; Lymphokie-activated T killer cells; Pallilative resection; Peripheral blood T lymphocytes; Tumor-bearing dogs