title: Effect of Spermidine Analogues on Cell Growth of Escherichia coli Polyamine Requiring Mutant MA261.
abstract: The effects of spermidine analogues [norspermidine (NSPD, 33), spermidine (SPD, 34), homospermidine (HSPD, 44) and aminopropylcadaverine (APCAD, 35)] on cell growth were studied using Escherichia coli polyamine-requiring mutant MA261. Cell growth was compared at 32°C, 37°C, and 42°C. All four analogues were taken up mainly by the PotABCD spermidine-preferential uptake system. The degree of stimulation of cell growth at 32°C and 37°C was NSPD ≥ SPD ≥ HSPD > APCAD, and SPD ≥ HSPD ≥ NSPD > APCAD, respectively. However, at 42°C, it was HSPD » SPD > NSPD > APCAD. One reason for this is HSPD was taken up effectively compared with other triamines. In addition, since natural polyamines (triamines and teteraamines) interact mainly with RNA, and the structure of RNA is more flexible at higher temperatures, HSPD probably stabilized RNA more tightly at 42°C. We have thus far found that 20 kinds of protein syntheses are stimulated by polyamines at the translational level. Among them, synthesis of OppA, RpoE and StpA was more strongly stimulated by HSPD at 42°C than at 37°C. Stabilization of the initiation region of oppA and rpoE mRNA was tighter by HSPD at 42°C than 37°C determined by circular dichroism (CD). The degree of polyamine stimulation of OppA, RpoE and StpA synthesis by NSPD, SPD and APCAD was smaller than that by HSPD at 42°C. Thus, the degree of stimulation of cell growth by spermidine analogues at the different temperatures is dependent on the stimulation of protein synthesis by some components of the polyamine modulon.
authors.affiliation: Faculty of Pharmacy, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba, 288-0025, Japan.
authors.forename: Taketo
authors.initials: T
authors.lastname: Yoshida
authors.affiliation: Faculty of Pharmacy, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba, 288-0025, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sakamoto
authors.affiliation: Faculty of Pharmacy, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba, 288-0025, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Terui
authors.affiliation: Laboratory of Bioorganic Chemistry, Department of Pharmaceutical Technology, Josai University, 1-1 Keyaki-dai, Sakado, Saitama, 350-0295, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Takao
authors.affiliation: Laboratory of Bioorganic Chemistry, Department of Pharmaceutical Technology, Josai University, 1-1 Keyaki-dai, Sakado, Saitama, 350-0295, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Sugita
authors.affiliation: Department of Frontier Bioscience, Hosei University, Koganei, Tokyo, 184-8584, Japan.
authors.forename: Kaneyoshi
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Department of Frontier Bioscience, Hosei University, Koganei, Tokyo, 184-8584, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ishihama
authors.affiliation: Amine Pharma Research Institute, Innovation Plaza at Chiba University, 1-8-15, Inohana, Chuo-ku, Chiba, Chiba 260-0856, Japan.|Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, Chiba, 260-8675, Japan.
authors.forename: Kazuei
authors.initials: K
authors.lastname: Igarashi
authors.affiliation: Faculty of Pharmacy, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba, 288-0025, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Kashiwagi
mesh_terms: D002103:Cadaverine / Q000031:analogs & derivatives / Q000494:pharmacology; D002352:Carrier Proteins / Q000096:biosynthesis; D049109:Cell Proliferation / Q000187:drug effects*; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis; D015964:Gene Expression Regulation, Bacterial; D008074:Lipoproteins / Q000096:biosynthesis; D018832:Molecular Chaperones / Q000096:biosynthesis; D050505:Mutant Proteins / Q000235:genetics; D011073:Polyamines / Q000378:metabolism; D012329:RNA, Bacterial / Q000187:drug effects / Q000235:genetics*; D012808:Sigma Factor / Q000096:biosynthesis; D013095:Spermidine / Q000031:analogs & derivatives / Q000494:pharmacology*
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; D018832:Molecular Chaperones; D050505:Mutant Proteins; C499290:OppA protein, E coli; D011073:Polyamines; D012329:RNA, Bacterial; D012808:Sigma Factor; C090104:StpA protein, E coli; C063202:sporulation-specific sigma factors; C006760:norspermidine; C038448:N-(3-aminopropyl)cadaverine; D002103:Cadaverine; D013095:Spermidine