title: Quantitative trait locus mapping of soybean maturity gene E5.
abstract: Time to flowering and maturity in soybean is controlled by loci E1 to E5, and E7 to E9. These loci were assigned to molecular linkage groups (MLGs) except for E5. This study was conducted to map the E5 locus using F2 populations expected to segregate for E5. F2 populations were subjected to quantitative trait locus (QTL) analysis for days to flowering (DF) and maturity (DM). In Harosoy-E5 × Clark-e2 population, QTLs for DF and DM were found at a similar position with E2. In Harosoy × Clark-e2E5 population, QTLs for DF and DM were found in MLG D1a and B1, respectively. In Harosoy-E5Dt2 × Clark-e2 population, a QTL for DF was found in MLG B1. Thus, results from these populations were not fully consistent, and no candidate QTL for E5 was found. In Harosoy × PI 80837 population, from which E5 was originally identified, QTLs corresponding to E1 and E3 were found, but none for E5 existed. Harosoy and PI 80837 had the e2-ns allele whereas Harosoy-E5 had the E2-dl allele. The E2-dl allele of Harosoy-E5 may have been generated by outcrossing and may be responsible for the lateness of Harosoy-E5. We conclude that a unique E5 gene may not exist.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518 , Japan.
authors.forename: Auchithya
authors.initials: A
authors.lastname: Dissanayaka
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518 , Japan.
authors.forename: Tito O
authors.initials: TO
authors.lastname: Rodriguez
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518 , Japan.
authors.forename: Shaokang
authors.initials: S
authors.lastname: Di
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518 , Japan.
authors.forename: Fan
authors.initials: F
authors.lastname: Yan
authors.affiliation: National Institute of Crop Science , 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518 , Japan.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Githiri
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518 , Japan.
authors.forename: Felipe Rojas
authors.initials: FR
authors.lastname: Rodas
authors.affiliation: Research Faculty of Agriculture, Hokkaido University , Sapporo, Hokkaido 060-8589 , Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Abe
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan; National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Takahashi
keywords: E5 gene; QTL mapping; maturity gene; soybean