title: A novel member of the Tombusviridae from a wild legume, Gompholobium preissii.
abstract: As part of an investigation into viruses of wild plants in Australia, a contiguous sequence of 3935 nucleotides was obtained after shotgun sequencing of RNA isolated from an asymptomatic wild legume, Gompholobium preissii. Phylogenetic analysis of the sequence revealed that it most closely resembled that of Trailing lespedeza virus 1 (TLV1), a virus isolated from a wild legume in America. The proposed virus, named Gompholobium virus A, and TLV1 are genetically closest to viruses in the genera Alphacarmovirus and Pelarspovirus, family Tombusviridae, but they share features distinguishing them from both groups.
authors.affiliation: Plant Biotechnology Group-Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, WA, 6150, Australia.
authors.forename: Shu Hui
authors.initials: SH
authors.lastname: Koh
authors.affiliation: Plant Biotechnology Group-Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, WA, 6150, Australia.
authors.forename: Jamie W L
authors.initials: JW
authors.lastname: Ong
authors.affiliation: School of Engineering and Information Technology, Mathematics and Statistics, Murdoch University, Perth, WA, 6150, Australia.
authors.forename: Ryan
authors.initials: R
authors.lastname: Admiraal
authors.affiliation: Plant Biotechnology Group-Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, WA, 6150, Australia.
authors.forename: Krishnapillai
authors.initials: K
authors.lastname: Sivasithamparam
authors.affiliation: Plant Biotechnology Group-Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, WA, 6150, Australia.
authors.forename: Michael G K
authors.initials: MG
authors.lastname: Jones
authors.affiliation: Plant Biotechnology Group-Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, WA, 6150, Australia. s.wylie@murdoch.edu.au.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Wylie
mesh_terms: D001315:Australia; D016000:Cluster Analysis; D007887:Fabaceae / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D019183:Tombusviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral
keywords: Australian plant viruses; Deep sequencing; Gompholobium virus A; Next-generation sequencing; Wild plant virus