title: Ozonation as an effective way to stabilize new kinds of fermentation media used in biotechnological production of liquid fuel additives.
abstract: BACKGROUND
  Intermediates from processing sugar beets are considered an attractive feedstock for ethanol fermentation due to their high fermentable sugar content. In particular, medium prepared from raw sugar beet juice seems to be suitable for use in fermentation processes, but it is microbiologically unstable and requires sterilization.
  
  
  RESULTS
  This study investigates the effect of ozone treatment on the activity of microbial cells from Bacillus subtilis, Leuconostoc mesenteroides, Geobacillus stearothermophilus, Candida vini, and Aspergillus brasiliensis in raw sugar beet juice. Raw sugar beet juice contaminated with 10(5) cfu/mL of the microbial strains was treated with gaseous ozone (ozone concentration in the oxygen stream 0.1 g O3/L O2, flow rate 6 L/h, 10-30 min, 18-20 °C). The number of microflora decreased to 0 cfu/mL after 30 min of ozone treatment in all studied samples.
  
  
  CONCLUSIONS
  Medium prepared from raw sugar beet juice and sterilized by ozonation is suitable for use in fermentation processes.
authors.affiliation: Faculty of Biotechnology and Food Sciences, Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171-173, 90-924 Lodz, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziugan
authors.affiliation: Faculty of Biotechnology and Food Sciences, Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171-173, 90-924 Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Balcerek
authors.affiliation: Faculty of Chemistry, Institute of General and Ecological Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.
authors.forename: Michal J
authors.initials: MJ
authors.lastname: Binczarski
authors.affiliation: Faculty of Biotechnology and Food Sciences, Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171-173, 90-924 Lodz, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Kregiel
authors.affiliation: Faculty of Biotechnology and Food Sciences, Institute of Technology and Food Analysis, Lodz University of Technology, Wolczanska 171-173, 90-924 Lodz, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Kucner
authors.affiliation: Faculty of Biotechnology and Food Sciences, Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171-173, 90-924 Lodz, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Kunicka-Styczynska
authors.affiliation: Faculty of Biotechnology and Food Sciences, Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171-173, 90-924 Lodz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pielech-Przybylska
authors.affiliation: Faculty of Biotechnology and Food Sciences, Institute of Food Chemistry, Lodz University of Technology, Wolczanska 171-173, 90-924 Lodz, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Smigielski
authors.affiliation: Faculty of Chemistry, Institute of General and Ecological Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.
authors.forename: Izabela A
authors.initials: IA
authors.lastname: Witonska
keywords: Bioethanol production; Ozonation; Raw sugar beet juice; Sterilization