title: Flexible Molybdenum Electrodes towards Designing Affinity Based Protein Biosensors.
abstract: Molybdenum electrode based flexible biosensor on porous polyamide substrates has been fabricated and tested for its functionality as a protein affinity based biosensor. The biosensor performance was evaluated using a key cardiac biomarker; cardiac Troponin-I (cTnI). Molybdenum is a transition metal and demonstrates electrochemical behavior upon interaction with an electrolyte. We have leveraged this property of molybdenum for designing an affinity based biosensor using electrochemical impedance spectroscopy. We have evaluated the feasibility of detection of cTnI in phosphate-buffered saline (PBS) and human serum (HS) by measuring impedance changes over a frequency window from 100 mHz to 1 MHz. Increasing changes to the measured impedance was correlated to the increased dose of cTnI molecules binding to the cTnI antibody functionalized molybdenum surface. We achieved cTnI detection limit of 10 pg/mL in PBS and 1 ng/mL in HS medium. The use of flexible substrates for designing the biosensor demonstrates promise for integration with a large-scale batch manufacturing process.
authors.affiliation: Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Rd., Richardson, TX 75080, USA. vxk121030@utdallas.edu.
authors.forename: Vikramshankar
authors.initials: V
authors.lastname: Kamakoti
authors.affiliation: Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Rd., Richardson, TX 75080, USA. axp107120@utdallas.edu.
authors.forename: Anjan
authors.initials: A
authors.lastname: Panneer Selvam
authors.affiliation: Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Rd., Richardson, TX 75080, USA. nxr123230@utdallas.edu.
authors.forename: Nandhinee
authors.initials: N
authors.lastname: Radha Shanmugam
authors.affiliation: EnLiSense LLC, 1813 Audubon Pond way, Allen, TX 75013, USA. sxm131031@utdallas.edu.
authors.forename: Sriram
authors.initials: S
authors.lastname: Muthukumar
authors.affiliation: Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Rd., Richardson, TX 75080, USA. shalini.prasad@utdallas.edu.
authors.forename: Shalini
authors.initials: S
authors.lastname: Prasad
mesh_terms: D000906:Antibodies; D015374:Biosensing Techniques / Q000379:methods / Q000592:standards; D058266:Dielectric Spectroscopy; D004566:Electrodes; D007118:Immunoassay / Q000379:methods / Q000592:standards; D008982:Molybdenum; D011506:Proteins; D019210:Troponin I
chemical_list: D000906:Antibodies; D011506:Proteins; D019210:Troponin I; D008982:Molybdenum
keywords: bioassay; cardiac troponin-I; flexible substrate; label-free biosensing; molybdenum