title: Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction.
abstract: Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen.
authors.affiliation: Departamento de Bioquímica y Biología Molecular e Instituto Andaluz de Biotecnología, Edificio Severo Ochoa (C6), Universidad de Córdoba Córdoba, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Amil-Ruiz
authors.affiliation: Departamento de Bioquímica y Biología Molecular e Instituto Andaluz de Biotecnología, Edificio Severo Ochoa (C6), Universidad de Córdoba Córdoba, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Garrido-Gala
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas Valencia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Gadea
authors.affiliation: Departamento de Bioquímica y Biología Molecular e Instituto Andaluz de Biotecnología, Edificio Severo Ochoa (C6), Universidad de Córdoba Córdoba, Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Blanco-Portales
authors.affiliation: Departamento de Arquitectura de Computación, Universidad de Málaga, Campus de Teatinos Málaga, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Muñoz-Mérida
authors.affiliation: Departamento de Arquitectura de Computación, Universidad de Málaga, Campus de Teatinos Málaga, Spain.
authors.forename: Oswaldo
authors.initials: O
authors.lastname: Trelles
authors.affiliation: Centro Andalusian Institute of Agricultural and Fishering Research and Training (IFAPA) Las Torres-Tomejil, CAPMA-Junta de Andalucía Sevilla, Spain.
authors.forename: Berta
authors.initials: B
authors.lastname: de Los Santos
authors.affiliation: Centro Andalusian Institute of Agricultural and Fishering Research and Training (IFAPA) Las Torres-Tomejil, CAPMA-Junta de Andalucía Sevilla, Spain.
authors.forename: Francisco T
authors.initials: FT
authors.lastname: Arroyo
authors.affiliation: Centro Andalusian Institute of Agricultural and Fishering Research and Training (IFAPA) Las Torres-Tomejil, CAPMA-Junta de Andalucía Sevilla, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Aguado-Puig
authors.affiliation: Centro Andalusian Institute of Agricultural and Fishering Research and Training (IFAPA) Las Torres-Tomejil, CAPMA-Junta de Andalucía Sevilla, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Romero
authors.affiliation: Departamento de Biología Vegetal, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos Málaga, Spain.
authors.forename: José-Ángel
authors.initials: JÁ
authors.lastname: Mercado
authors.affiliation: Departamento de Biología Vegetal, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos Málaga, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Pliego-Alfaro
authors.affiliation: Departamento de Bioquímica y Biología Molecular e Instituto Andaluz de Biotecnología, Edificio Severo Ochoa (C6), Universidad de Córdoba Córdoba, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Muñoz-Blanco
authors.affiliation: Departamento de Bioquímica y Biología Molecular e Instituto Andaluz de Biotecnología, Edificio Severo Ochoa (C6), Universidad de Córdoba Córdoba, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Caballero
keywords: Colletotrichum acutatum; Fragaria × ananassa; quantification of gene expression; salicylic and jasmonic acid; strawberry defense response