title: In vivo analysis of septin heteropolymer rods and higher-order structures in filamentous fungi.
abstract: Individual septins from separate phylogenetic groups assemble into heteropolymer rods with defined compositions. Heteropolymer rods can in turn assemble into larger higher-order structures (HOS). Using a combination of immunoprecipitation and fluorescence microscopy in Aspergillus nidulans wild-type and septin-mutant strains, we have shown that even when septin HOS are not visible by fluorescence microscopy, heteropolymer rods can still be present and that a septin found largely in filamentous fungi is required for a subset of HOS. We raise several factors that are important for analysis of heteropolymer rods and HOS.
authors.affiliation: University of Georgia, Athens, GA, United States.
authors.forename: A
authors.initials: A
authors.lastname: Smith
authors.affiliation: University of Georgia, Athens, GA, United States.
authors.forename: M
authors.initials: M
authors.lastname: Momany
mesh_terms: D001233:Aspergillus nidulans / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000737:chemistry / Q000235:genetics; D047468:Immunoprecipitation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010802:Phylogeny; D058105:Polymerization; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics; D058112:Septins / Q000737:chemistry* / Q000235:genetics
chemical_list: D058112:Septins
keywords: Aspergillus nidulans; HOS; Heteropolymers; S-tag septin; Saccharomyces cerevisiae; Septins