title: Mass Spectrometric Imaging of Wheat (Triticum spp.) and Barley (Hordeum vulgare L.) Cultivars: Distribution of Major Cell Wall Polysaccharides According to Their Main Structural Features.
abstract: Arabinoxylans (AX) and (1→3),(1→4)-β-glucans (BG) are the main components of cereal cell walls and influence many aspects of their end uses. Important variations in the composition and structure of these polysaccharides have been reported among cereals and cultivars of a given species. In this work, the spatial distribution of AX and BG in the endosperm of mature grains was established for nine wheat varieties and eight barley varieties using enzymatically assisted mass spectrometry imaging (MSI). Important structural features of the AX and BG polymers that were previously shown to influence their physicochemical properties were assessed. Differences in the distribution of AX and BG structures were observed, both within the endosperm of a given cultivar and between wheat and barley cultivars. This study provides a unique picture of the structural heterogeneity of AX and BG polysaccharides at the scale of the whole endosperm in a series of wheat and barley cultivars. Thus, it can participate meaningfully in a strategy aiming at understanding the structure-function relationships of these two polymers.
authors.affiliation: INRA , UR1268 Biopolymers Interactions Assemblies, F-44316 Nantes, France.
authors.forename: Dušan
authors.initials: D
authors.lastname: Veličković
authors.affiliation: INRA , UR1268 Biopolymers Interactions Assemblies, F-44316 Nantes, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Saulnier
authors.affiliation: INRA , UR1268 Biopolymers Interactions Assemblies, F-44316 Nantes, France.
authors.forename: Margot
authors.initials: M
authors.lastname: Lhomme
authors.affiliation: INRA , UR1268 Biopolymers Interactions Assemblies, F-44316 Nantes, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Damond
authors.affiliation: INRA , UR1268 Biopolymers Interactions Assemblies, F-44316 Nantes, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Guillon
authors.affiliation: INRA , UR1268 Biopolymers Interactions Assemblies, F-44316 Nantes, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Rogniaux
mesh_terms: D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism*; D056625:Endosperm / Q000737:chemistry* / Q000378:metabolism; D001467:Hordeum / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D014908:Triticum / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011134:Polysaccharides
keywords: MALDI-MS imaging; arabinoxylans; barley (Hordeum vulgare); beta-glucans; cell wall; plant; polysaccharides; wheat (Triticum)