title: Relation between clinical mature and immature lymphocyte cells in human peripheral blood and their spatial label free scattering patterns.
abstract: A single living cell's light scattering pattern (LSP) in the horizontal plane, which has been denoted as the cell's "2D fingerprint," may provide a powerful label-free detection tool in clinical applications. We have recently studied the LSP in spatial scattering planes, denoted as the cell's "3D fingerprint," for mature and immature lymphocyte cells in human peripheral blood. The effects of membrane size, morphology, and the existence of the nucleus on the spatial LSP are discussed. In order to distinguish clinical label-free mature and immature lymphocytes, the special features of the spatial LSP are studied by statistical method in both the spatial and frequency domains. Spatial LSP provides rich information on the cell's morphology and contents, which can distinguish mature from immature lymphocyte cells and hence ultimately it may be a useful label-free technique for clinical leukemia diagnosis.
authors.affiliation: State Key Laboratory for Manufacturing System Engineering, School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Manufacturing System Engineering, School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.
authors.forename: Zhenxi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Manufacturing System Engineering, School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000473:pathology*; D002462:Cell Membrane / Q000473:pathology*; D005260:Female; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000175:diagnosis / Q000473:pathology; D008027:Light; D008297:Male; D008875:Middle Aged; D012542:Scattering, Radiation; D013601:T-Lymphocytes / Q000473:pathology*