title: The HPV16 E7 Oncoprotein Disrupts Dendritic Cell Function and Induces the Systemic Expansion of CD11b(+)Gr1(+) Cells in a Transgenic Mouse Model.
abstract: Objective. The aim of this study was to analyze the effects of the HPV16 E7 oncoprotein on dendritic cells (DCs) and CD11b(+)Gr1(+) cells using the K14E7 transgenic mouse model. Materials and Methods. The morphology of DCs was analyzed in male mouse skin on epidermal sheets using immunofluorescence and confocal microscopy. Flow cytometry was used to determine the percentages of DCs and CD11b(+)Gr1(+) cells in different tissues and to evaluate the migration of DCs. Results. In the K14E7 mouse model, the morphology of Langerhans cells and the migratory activity of dendritic cells were abnormal. An increase in CD11b(+)Gr1(+) cells was observed in the blood and skin of K14E7 mice, and molecules related to CD11b(+)Gr1(+) chemoattraction (MCP1 and S100A9) were upregulated. Conclusions. These data suggest that the HPV16 E7 oncoprotein impairs the function and morphology of DCs and induces the systemic accumulation of CD11b(+)Gr1(+) cells.
authors.affiliation: Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), 07360 Mexico City, CDMX, Mexico.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Damian-Morales
authors.affiliation: Unit of Health Sciences, Faculty of Odontology, Universidad Autónoma de Baja California, 21100 Mexicali, BC, Mexico.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Serafín-Higuera
authors.affiliation: Research Unit, Hospital Juárez de México, 07760 Mexico City, CDMX, Mexico.
authors.forename: Mario Adán
authors.initials: MA
authors.lastname: Moreno-Eutimio
authors.affiliation: Research Unit, Hospital Juárez de México, 07760 Mexico City, CDMX, Mexico.
authors.forename: Enoc M
authors.initials: EM
authors.lastname: Cortés-Malagón
authors.affiliation: Research Unit, Hospital Juárez de México, 07760 Mexico City, CDMX, Mexico.
authors.forename: José
authors.initials: J
authors.lastname: Bonilla-Delgado
authors.affiliation: Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), 07360 Mexico City, CDMX, Mexico.
authors.forename: Genaro
authors.initials: G
authors.lastname: Rodríguez-Uribe
authors.affiliation: Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), 07360 Mexico City, CDMX, Mexico.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Ocadiz-Delgado
authors.affiliation: McArdle Laboratory for Cancer Research, University of Wisconsin, School of Medicine and Public Health, Madison, WI 53726, USA.
authors.forename: Paul F
authors.initials: PF
authors.lastname: Lambert
authors.affiliation: Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), 07360 Mexico City, CDMX, Mexico.
authors.forename: Patricio
authors.identifier: 0000-0003-4603-3249
authors.initials: P
authors.lastname: Gariglio
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism*; D002452:Cell Count; D002465:Cell Movement; D049109:Cell Proliferation; D048430:Cell Shape; D003713:Dendritic Cells / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D004817:Epidermis / Q000473:pathology; D006965:Hyperplasia; D037181:Lectins, C-Type / Q000378:metabolism; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D037241:Mannose-Binding Lectins / Q000378:metabolism; D008822:Mice, Transgenic; D023421:Models, Animal; D050725:Papillomavirus E7 Proteins / Q000378:metabolism*; D013154:Spleen / Q000473:pathology
chemical_list: D000954:Antigens, Surface; D039481:CD11b Antigen; C498497:Cd207 protein, mouse; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16