title: Chemical and Instrumental Approaches for Capillary Electrophoresis (CE)-Fluorescence Analysis of Proteins.
abstract: Capillary electrophoresis (CE) coupled to fluorescence detection is an invaluable technique for the quantitative analysis of proteins of interest in the field of clinical diagnosis and quality control of novel biotechnology products. The various chemical and instrumental approaches that have been reported to carry out such sensitive analysis are described in this paper. To illustrate the contribution of CE to the analysis of therapeutic proteins, a detailed protocol for impurities profiling of a recombinant antibody sample using CE-LEDIF is given.
authors.affiliation: Institut Galien Paris Sud, UMR8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, 5 rue Jean Baptiste Clément, Châtenay-Malabry, 92290, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Le Potier
authors.affiliation: Picometrics Technologies, 478 rue de la Découverte, Labège, 31670, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Boutonnet
authors.affiliation: IPBS, Université Paul Sabatier, Université de Toulouse, 205 Route de Narbonne, Toulouse, 31062, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Ecochard
authors.affiliation: Laboratoire des IMRCP, Université Paul Sabatier, Université de Toulouse, 118 Route de Narbonne, Toulouse, 31062, France. couderc@chimie.ups-tlse.fr.
authors.forename: François
authors.initials: F
authors.lastname: Couderc
mesh_terms: D000906:Antibodies / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D005453:Fluorescence; D006801:Humans; D011994:Recombinant Proteins / Q000302:isolation & purification; D013194:Staining and Labeling
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins
keywords: Capillary electrophoresis; Labeling procedures; Laser-induced fluorescence; Light-emitting diodes; Proteins