title: Rapid Bedside Inactivation of Ebola Virus for Safe Nucleic Acid Tests.
abstract: Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport, and high-throughput or rapid diagnostic testing during an outbreak. We show that the commercially available Magna Pure lysis/binding buffer used for nucleic acid extraction inactivates Ebola virus. A rapid bedside inactivation method for nucleic acid tests is obtained by simply adding Magna Pure lysis/binding buffer directly into vacuum blood collection EDTA tubes using a thin needle and syringe prior to sampling. The ready-to-use inactivation vacuum tubes are stable for more than 4 months, and Ebola virus RNA is preserved in the Magna Pure lysis/binding buffer for at least 5 weeks independent of the storage temperature. We also show that Ebola virus RNA can be manually extracted from Magna Pure lysis/binding buffer-inactivated samples using the QIAamp viral RNA minikit. We present an easy and convenient method for bedside inactivation using available blood collection vacuum tubes and reagents. We propose to use this simple method for fast, safe, and easy bedside inactivation of Ebola virus for safe transport and routine nucleic acid detection.
authors.affiliation: Virus Research & Development Laboratory Statens Serum Institut, Copenhagen, Denmark mwr@ssi.dk.
authors.forename: Maiken Worsøe
authors.initials: MW
authors.lastname: Rosenstierne
authors.affiliation: Public Health Agency of Sweden, Solna, Sweden.
authors.forename: Helen
authors.initials: H
authors.lastname: Karlberg
authors.affiliation: National Influenza Centre Norway, Norwegian Institute of Public Health, Oslo, Norway.
authors.forename: Karoline
authors.initials: K
authors.lastname: Bragstad
authors.affiliation: Public Health Agency of Sweden, Solna, Sweden.
authors.forename: Gunnel
authors.initials: G
authors.lastname: Lindegren
authors.affiliation: Public Health Agency of Sweden, Solna, Sweden.
authors.forename: Malin Lundahl
authors.initials: ML
authors.lastname: Stoltz
authors.affiliation: Department of Molecular Medicine, University of Padova, Padua, Italy.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Salata
authors.affiliation: Department of Microbiology, Oslo University Hospital, Oslo, Norway.
authors.forename: Anne-Marte Bakken
authors.initials: AM
authors.lastname: Kran
authors.affiliation: National Influenza Centre Norway, Norwegian Institute of Public Health, Oslo, Norway.
authors.forename: Susanne Gjeruldsen
authors.initials: SG
authors.lastname: Dudman
authors.affiliation: Public Health Agency of Sweden, Solna, Sweden Karolinska Institutet, Solna, Sweden.
authors.forename: Ali
authors.initials: A
authors.lastname: Mirazimi
authors.affiliation: Virus Research & Development Laboratory Statens Serum Institut, Copenhagen, Denmark Infectious Disease Research Unit, University of Southern Denmark, Odense, Denmark.
authors.forename: Anders
authors.initials: A
authors.lastname: Fomsgaard
mesh_terms: D004203:Disinfection / Q000379:methods*; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis* / Q000821:virology*; D006801:Humans; D019095:Point-of-Care Systems; D012367:RNA, Viral / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D038301:Virus Inactivation
chemical_list: D012367:RNA, Viral