title: Rapid Preparation of a Plasma Membrane Fraction: Western Blot Detection of Translocated Glucose Transporter 4 from Plasma Membrane of Muscle and Adipose Cells and Tissues.
abstract: Membrane proteins account for 70% to 80% of all pharmaceutical targets, indicating their clinical relevance and underscoring the importance of identifying differentially expressed membrane proteins that reflect distinct disease properties. The translocation of proteins from the bulk of the cytosol to the plasma membrane is a critical step in the transfer of information from membrane-embedded receptors or transporters to the cell interior. To understand how membrane proteins work, it is important to separate the membrane fraction of cells. This unit provides a protocol for rapidly obtaining plasma membrane fractions for western blot analysis. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Research & Development Institute, House Wellness Foods Corporation, Itami, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yamashita
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, Kobe, Japan.
authors.forename: Yasukiyo
authors.initials: Y
authors.lastname: Yoshioka
authors.affiliation: Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Nishiumi
authors.affiliation: Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ashida
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000737:chemistry; D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry*; D002478:Cells, Cultured; D051275:Glucose Transporter Type 4 / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D032342:Muscle Cells / Q000737:chemistry; D018482:Muscle, Skeletal / Q000166:cytology; D051381:Rats
chemical_list: D051275:Glucose Transporter Type 4; D008565:Membrane Proteins
keywords: membrane fraction; membrane protein; plasma membrane; protein extraction; western blotting