title: In vitro and in vivo assays on egg white/polyvinyl alcohol/clay nanocomposite hydrogel wound dressings.
abstract: Novel nanocomposite hydrogel wound dressings on the basis of egg white and polyvinyl alcohol, as matrix, and natural Na-montmorillonite clay, as reinforcing agent, were prepared and their performances on wound healing investigated in vitro and in vivo. In vitro cytotoxicity assay revealed non-cytotoxic activity and excellent biocompatibility level of prepared nanocomposite hydrogel wound dressings. The bacterial penetration assay showed the prepared nanocomposite hydrogel wound dressings are excellent barriers against microorganisms and could protect the wound from infection during the wound healing. In vivo animal study showed that the wound healing process was considerably faster in wounds covered with nanocomposite hydrogel wound dressings compared to the conventional wound dressing, i.e. sterile gauze, due to creation of a moist environment on the wound surface and faster migration rate of the epidermal cells. The mechanical properties of healed wounds with nanocomposite hydrogel wound dressings were better than those control wounds covered with sterile gauze due to their better collagen formation ability as a result of created moist healing condition as well as the presence of egg white, as a source of proteins, in their structures.
authors.affiliation: a Faculty of Chemical Engineering , Urmia University of Technology , Urmia , Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Sirousazar
authors.affiliation: a Faculty of Chemical Engineering , Urmia University of Technology , Urmia , Iran.
authors.forename: Azinsadat
authors.initials: A
authors.lastname: Jahani-Javanmardi
authors.affiliation: a Faculty of Chemical Engineering , Urmia University of Technology , Urmia , Iran.
authors.forename: Farshad
authors.initials: F
authors.lastname: Kheiri
authors.affiliation: b Faculty of Medical Sciences, Immunology Department , Tarbiat Modares University , Tehran , Iran.
authors.forename: Zuhair Muhammad
authors.initials: ZM
authors.lastname: Hassan
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry*; D000818:Animals; D001458:Bandages / Q000382:microbiology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity; D000077215:Clay; D004529:Egg White / Q000737:chemistry*; D005260:Female; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000538:Aluminum Silicates; D001672:Biocompatible Materials; D020100:Hydrogels; D011142:Polyvinyl Alcohol; D000077215:Clay
keywords: Hydrogel wound dressing; clay; egg white; in vivo study; nanocomposite; polyvinyl alcohol