title: Protein patterning by a DNA origami framework.
abstract: A spatial arrangement of proteins provides structural and functional advantages in vast technological applications as well as fundamental research. Most protein patterning procedures employ complicated, time consuming and very costly nanofabrication techniques. As an alternative route, we developed a fully biomolecular self-assembly method using DNA Origami Frames (DOF) as a template for both small and large scale protein patterning. We employed a triangular DOF (tDOF) to arrange the Bovine Serum Albumin (BSA) protein. Our in situ protein patterning strategy provides a novel, fully organic platform using a fast and low-cost surface approach with possible utilization in fundamental science and technological applications.
authors.affiliation: Center for DNA Nanotechnology (CDNA) and Interdisciplinary Nanoscience (iNANO) Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark. dong@inano.au.dk.
authors.forename: Hüsnü
authors.initials: H
authors.lastname: Aslan
authors.affiliation: Center for DNA Nanotechnology (CDNA) and Interdisciplinary Nanoscience (iNANO) Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark. dong@inano.au.dk.
authors.forename: Abhichart
authors.initials: A
authors.lastname: Krissanaprasit
authors.affiliation: Center for DNA Nanotechnology (CDNA) and Interdisciplinary Nanoscience (iNANO) Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark. dong@inano.au.dk.
authors.forename: Flemming
authors.initials: F
authors.lastname: Besenbacher
authors.affiliation: Center for DNA Nanotechnology (CDNA) and Interdisciplinary Nanoscience (iNANO) Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark. dong@inano.au.dk.
authors.forename: Kurt V
authors.initials: KV
authors.lastname: Gothelf
authors.affiliation: Center for DNA Nanotechnology (CDNA) and Interdisciplinary Nanoscience (iNANO) Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark. dong@inano.au.dk.
authors.forename: Mingdong
authors.initials: M
authors.lastname: Dong
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D012710:Serum Albumin, Bovine; D004247:DNA