title: Molecular diagnostic testing for primary biliary cholangitis.
abstract: A reliable liver autoimmune serology for the diagnosis of primary biliary cholangitis (PBC) is of particular importance. Recognition of patients at early stages and prompt treatment initiation may alter the outcome, slow progression, delays liver failure, and improves survival.
  
  
  
  In this review, we summarize and discuss the published data obtained from literature searches from PubMed and The National Library of Medicine (USA) and our own experience on the current and potential molecular based approaches to the diagnosis of PBC. Expert commentary: Standardization of liver diagnostic serology and clinical governance are two major points as antimitochondrial antibodies are the diagnostic hallmark of the disease and PBC-specific antinuclear antibodies could assist in the diagnosis and estimation of prognosis. New biomarkers such as novel autoantibodies, genetic polymorphisms, metabolomic profiling, micro-RNA and epigenetics may assist to the understanding, diagnosis and management of the disease.
authors.affiliation: a Department of Medicine and Research Laboratory of Internal Medicine, Medical School , University of Thessaly , Larissa , Greece.
authors.forename: Nikolaos K
authors.initials: NK
authors.lastname: Gatselis
authors.affiliation: a Department of Medicine and Research Laboratory of Internal Medicine, Medical School , University of Thessaly , Larissa , Greece.
authors.forename: George N
authors.initials: GN
authors.lastname: Dalekos
mesh_terms: D001327:Autoimmune Diseases / Q000097:blood / Q000175:diagnosis / Q000235:genetics; D015415:Biomarkers / Q000097:blood; D002761:Cholangitis / Q000097:blood / Q000175:diagnosis / Q000235:genetics; D006801:Humans; D017093:Liver Failure / Q000097:blood / Q000175:diagnosis / Q000235:genetics / Q000517:prevention & control; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D025202:Molecular Diagnostic Techniques / Q000379:methods / Q000639:trends
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: ELISA; Primary biliary cholangitis; antimitochondrial antibodies; antinuclear antibodies; epigenetics; genetics; immunoblotting; indirect immunofluorescence; metabolomics; multiplexed assays