title: Chemokine and Chemokine Receptor Profiles in Metastatic Salivary Adenoid Cystic Carcinoma.
abstract: OBJECTIVE
  To characterize the chemokine pattern in metastatic salivary adenoid cystic carcinoma (SACC).
  
  
  METHODS
  Real-time polymerase chain reaction (RT-PCR) was used to compare chemokine and chemokine receptor gene expression in two SACC cell lines: SACC-83 and SACC-LM (lung metastasis). Chemokines and receptor genes were then screened and their expression pattern characterized in human tissue samples of non-recurrent SACC and recurrent SACC with perineural invasion.
  
  
  RESULTS
  Expression of chemokine receptors C5AR1, CCR1, CCR3, CCR6, CCR7, CCR9, CCR10, CXCR4, CXCR6, CXCR7, CCRL1 and CCRL2 were higher in SACC-83 compared to SACC-LM. CCRL1, CCBP2, CMKLR1, XCR1 and CXCR2 and 6 chemokine genes (CCL13, CCL27, CXCL14, CMTM1, CMTM2, CKLF) were more highly expressed in tissues of patients without tumor recurrence/perineural invasion compared to those with tumor recurrence. CCRL1 (receptor), CCL27, CMTM1, CMTM2, and CKLF (chemokine) genes were more highly expressed in SACC-83 and human tissues of patients without tumor recurrence/perineural invasion.
  
  
  CONCLUSIONS
  CCRL1, CCL27, CMTM1, CMTM2 and CKLF may play important roles in the development of tumor metastases in SACC.
authors.affiliation: Department of Otolaryngology, Wake Forest School of Medicine, Winston Salem, NC, U.S.A.
authors.forename: Ashley C
authors.initials: AC
authors.lastname: Mays
authors.affiliation: Department of Otolaryngology, Wake Forest School of Medicine, Winston Salem, NC, U.S.A.
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Otolaryngology, Wake Forest School of Medicine, Winston Salem, NC, U.S.A.
authors.forename: James D
authors.initials: JD
authors.lastname: Browne
authors.affiliation: Department of Otolaryngology, Wake Forest School of Medicine, Winston Salem, NC, U.S.A. csulliva@wakehealth.edu.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Sullivan
mesh_terms: D000368:Aged; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D018925:Chemokines / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000556:secondary; D008297:Male; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis; D019707:Receptors, Chemokine / Q000096:biosynthesis* / Q000235:genetics; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D018925:Chemokines; D019707:Receptors, Chemokine
keywords: Adenoid cystic carcinoma; distant metastasis; metastatic salivary adenoid cystic carcinoma; salivary tumors