title: Multiparametric magnetic resonance imaging including oxygenation mapping of experimental ischaemic stroke.
abstract: Recent advances in MRI methodology, such as microvascular and brain oxygenation (StO2) imaging, may prove useful in obtaining information about the severity of the acute stroke. We assessed the potential of StO2to detect the ischaemic core in the acute phase compared to apparent diffusion coefficient and to predict the final necrosis. Sprague-Dawley rats (n = 38) were imaged during acute stroke (D0) and 21 days after (D21). A multiparametric MRI protocol was performed at 4.7T to characterize brain damage within three region of interest: 'LesionD0' (diffusion), 'Mismatch' representing penumbra (perfusion/diffusion) and 'Hypoxia' (voxels < 40% of StO2within the region of interest LesionD0). Voxel-based analysis of stroke revealed heterogeneity of the region of interest LesionD0, which included voxels with different degrees of oxygenation decrease. This finding was supported by a dramatic decrease of vascular and perfusion parameters within the region of interest hypoxia. This zone presented the lowest values of almost all parameters analysed, indicating a higher severity. Our study demonstrates the potential of StO2magnetic resonance imaging to more accurately detect the ischaemic core without the inclusion of any reversible ischaemic damage. Our follow-up study indicates that apparent diffusion coefficient imaging overestimated the final necrosis while StO2imaging did not.
authors.affiliation: 1 Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.|2 Inserm, U1216, Grenoble, France.|3 CAPES Foundation, Ministry of Education of Brazil, Brasilia, Brazil.
authors.forename: Ligia Simões Braga
authors.initials: LSB
authors.lastname: Boisserand
authors.affiliation: 1 Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.|2 Inserm, U1216, Grenoble, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Lemasson
authors.affiliation: 1 Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.|2 Inserm, U1216, Grenoble, France.|4 Bruker Biospin, Ettlingen, Germany.
authors.forename: Lydiane
authors.initials: L
authors.lastname: Hirschler
authors.affiliation: 1 Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.|2 Inserm, U1216, Grenoble, France.|5 Cell Therapy and Engineering Unit, EFS Rhône Alpes, Saint Ismier, France.
authors.forename: Anaïck
authors.initials: A
authors.lastname: Moisan
authors.affiliation: 1 Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.
authors.forename: Violaine
authors.initials: V
authors.lastname: Hubert
authors.affiliation: 1 Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.|2 Inserm, U1216, Grenoble, France.
authors.forename: Emmanuel L
authors.initials: EL
authors.lastname: Barbier
authors.affiliation: 1 Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.|2 Inserm, U1216, Grenoble, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Rémy
authors.affiliation: 1 Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.|2 Inserm, U1216, Grenoble, France.|6 CHU Grenoble Alpes, Grenoble, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Detante
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000503:physiopathology; D002545:Brain Ischemia / Q000150:complications / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000000981:diagnostic imaging / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D010100:Oxygen
keywords: magnetic resonance imaging; mqBOLD; oxygenation; penumbra; stroke