title: Prediction of the antiglycation activity of polysaccharides from Benincasa hispida using a response surface methodology.
abstract: Benincasa hispida is a popular vegetable in China. Our previous experiments suggested that polysaccharides isolated from B. hispida fruits (PBH) have antiglycation effect and DPPH free radical scavenging activity. Ultrasonic treatments can be used to extract polysaccharides from Benincasa hispida (PBH). The aim of this study was to investigate the correlation between the ultrasonic treatment conditions and the antiglycation activity of PBH. A mathematical model was generated with an artificial neural network (ANN) toolbox from MATLAB to analyze the effects of ultrasonic treatment conditions on antiglycation activity. The response surface plots showed relationships between ultrasonic extraction conditions and bioactivity. The R(2) value of the model was 0.9919, which suggested good fitness of the neural network. The application of genetic algorithms showed that the optimal ultrasonic extraction conditions resulted in the highest antiglycation activity for PBH. These were 150W, 46°C, and 35min. These conditions produced a predicted antiglycation activity of 41.2%; the actual activity was 40.9% under optimal conditions. This is very close to the predicted value. The experimental data indicated that the PBH possessed both antiglycation and antioxidant activities. The maximum actual value of antiglycation was 101.7% that of the positive control, and the PBH inhibited the DPPH free radicals with an EC50 value of 0.98mg/mL. This is 66.2% that of ascorbic acid. These results explained the observations that B. hispida can decrease glucose levels in diabetic patients. The experimental results also showed that the ANN could be used for optimization and prediction.
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Jiang
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Kuang
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
authors.forename: Fansheng
authors.initials: F
authors.lastname: Kong
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China. Electronic address: ycybridge@163.com.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Yan
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005591:Chemical Fractionation / Q000379:methods*; D019662:Cucurbitaceae / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D006031:Glycosylation / Q000187:drug effects; D008962:Models, Theoretical; D016571:Neural Networks, Computer; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000069453:Ultrasonic Waves
chemical_list: D000975:Antioxidants; D011134:Polysaccharides
keywords: Aminoguanidine (PubChem CID: 2146); Antiglycation activity; Ascorbic Acid (PubChem CID: 54670067); Benincasa hispida; DPPH Free Radical (PubChem CID: 2735032); Disodium Hydrogen Phosphate (PubChem CID: 24203); Glucose (PubChem CID: 5793); Methanol (PubChem CID: 887); Monosodium Phosphate (PubChem CID: 23672064); Na(2)HPO(4); NaH(2)PO(4); Phenol (PubChem CID: 996); Polysaccharides; Response surface methodology; Sodium Azide (PubChem CID: 33557); Sulfuric Acid (PubChem CID: 1118); Ultrasonic extraction