title: [Brain Protection of Muscone in Rats with Brain Injury].
abstract: OBJECTIVE
  To observe cerebral protective effect of muscone (nasal administration) on traumatic brain injury model rats.
  
  
  METHODS
  SD rats were divided into the sham-operation group, the model group, and the treatment groups according to random digit table, 50 in each group. Traumatic brain injury model was established by controlled cortical strike. Rats in the sham-operation group received surgery and anesthesia procedures only, with no strike. Muscone (1.8 mg/kg) was delivered to rats in the treatment group using in situ nasal perfusion, 30 min each time, twice daily for 7 successive days. Water content of brain tissue was detected in each group before intervention (T1), at day 3 of intervention (T2), day 5 of intervention (T3), and after intervention (T4), respectively. Expression levels of brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) were detected using immunohistochemical analysis.
  
  
  RESULTS
  Compared with the sham-operated group, water content of brain tissue increased (P < 0.05), and expression levels of NGF and BDNF decreased in the model group at T1, T2, T3, and T4 (P <0. 01). Compared with the model group, water content of brain tissue decreased (P < 0.05), and expression levels of NGF and BDNF increased (P < 0.01) in the treatment group at T1, T2, and T3.
  
  
  CONCLUSIONS
  Nasal administration of muscone could reduce water content of brain tissue, alleviate cerebral edema, promote secretion of BDNF and NGF by olfactory ensheathing cells in traumatic brain injury rats.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Li-fa
authors.initials: LF
authors.lastname: Huang
authors.forename: Shui-jing
authors.initials: SJ
authors.lastname: Zhou
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Cui
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ye
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects; D001930:Brain Injuries / Q000188:drug therapy*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D003516:Cycloparaffins / Q000494:pharmacology*; D020932:Nerve Growth Factor / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D003516:Cycloparaffins; D020932:Nerve Growth Factor; C031021:muscone