title: Formation and properties of hyaluronan/nano Ag and hyaluronan-lecithin/nano Ag films.
abstract: A facile and environmentally friendly method of the preparation of silver nanoparticles embedded in hyaluronan (Hyal/Ag) and hyaluronan-lecithin (Hyal-L/Ag) matrix was developed. Thin, elastic foils were prepared from gels by an in situ synthesis of Ag in an aqueous solution of sodium hyaluronate (Hyal), using aq. d-(+)-xylose solution as a reducing agent. The gels were applied to a clean, smooth, defatted Teflon surface and left for drying in the air. The dry foils were stored in a closed container. UV-vis spectroscopy, transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) spectra confirmed formation of about 10nm ball-shaped Ag nanoparticles situated within the polysaccharide template. Thermal properties of the composites were characterized involving differential scanning calorimetry (DSC) and thermogravimetric (TGA) analyses, whereas molecular weights of polysaccharide chains of the matrix were estimated with the size exclusion chromatography coupled with multiangle laser light scattering and refractometric detectors (HPSEC-MALLS-RI). An increase in the molecular weight of the hyaluronate after generation of Ag nanoparticles was observed. The foils showed specific properties. The study confirmed that silver nanoparticles can be successfully prepared with environmentally friendly method, using hyaluronan as a stabilizing template. Hyaluronan and hyaluronan-lecithin matrices provide nanocrystals uniform in size and shape. The composites demonstrated a bacteriostatic activity.
authors.affiliation: Department of Chemistry and Physics, Agricultural University, Balicka Street 122, 30-149 Krakow, Poland.
authors.forename: Gohar
authors.initials: G
authors.lastname: Khachatryan
authors.affiliation: Department of Chemistry and Physics, Agricultural University, Balicka Street 122, 30-149 Krakow, Poland. Electronic address: rrchacza@cyf-kr.edu.pl.
authors.forename: Karen
authors.initials: K
authors.lastname: Khachatryan
authors.affiliation: Department of Microbiology, Agricultural University, Mickiewicz ave. 24/28, 30-059 Krakow, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Grzyb
authors.affiliation: Department of Chemistry and Physics, Agricultural University, Balicka Street 122, 30-149 Krakow, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Fiedorowicz
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D055772:Green Chemistry Technology; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology; D054709:Lecithins / Q000737:chemistry / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D012834:Silver / Q000737:chemistry / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D054709:Lecithins; D012834:Silver; D006820:Hyaluronic Acid
keywords: Biocomposites; Hyaluronic acid; Lecithin; Nanometals; Nanosilver