title: MicroRNA Profiling from RSV-Infected Biofluids, Whole Blood, and Tissue Samples.
abstract: Several studies have shown that respiratory syncytial virus (RSV) can modulate the host innate immune response by dysregulation of host microRNAs (miRNAs) related to the antiviral response, a feature that also affects the memory immune response to RSV (Thornburg et al. MBio 3(6), 2012). miRNAs are small, endogenous, noncoding RNAs that function in posttranscriptional gene regulation. Here, we explain a compilation of methods for the purification, quantification, and characterization of miRNA expression profiles in biofluids, whole blood samples, and tissue samples obtained from in vivo studies. In addition, this chapter describes methods for the isolation of exosomal miRNA populations. Understanding alterations in miRNA expression profiles and identifying miRNA targets genes, and their contribution to the pathogenesis of RSV, may help elucidate novel mechanism of host-virus interaction (Rossi et al., Pediatr Pulmonol, 2015).
authors.affiliation: Animal Health Research Center, Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, 111 Carlton Street, Athens, GA, 30602, USA.
authors.forename: Lydia
authors.initials: L
authors.lastname: Anderson
authors.affiliation: Animal Health Research Center, Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, 111 Carlton Street, Athens, GA, 30602, USA.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Jorquera
authors.affiliation: Animal Health Research Center, Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, 111 Carlton Street, Athens, GA, 30602, USA. ratripp@uga.edu.
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Tripp
mesh_terms: D001201:Ascites / Q000235:genetics; D055354:Exosomes / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007113:Immunity, Innate; D035683:MicroRNAs / Q000032:analysis / Q000097:blood / Q000235:genetics*; D018357:Respiratory Syncytial Virus Infections / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics* / Q000652:urine; D018113:Respiratory Syncytial Virus, Human / Q000472:pathogenicity
chemical_list: D035683:MicroRNAs
keywords: Exosomes; MicroRNAs; RNA isolation; RT-qPCR; Respiratory syncytial virus