title: Evaluation of anxiolytic-like activity of Vitis vinifera juice in mice.
abstract: OBJECTIVE
  Scientific studies have shown that Vitis vinifera (V. vinifera) contains flavonoids and stillbenoids. Flavonoids are well known to possess anxiolytic activities. In view of the idea that flavonoids present in V. vinifera could be useful in anxiety, we evaluated anxiolytic-like activity of V. vinifera juice (VVJ).
  
  
  METHODS
  Light/dark box and the open field test were used to assess the anxiolytic potential of V. vinifera juice (VVJ). The juice was given orally by gavage at the dose of 4 and 8 mL/kg body weight. Diazepam (1 mg/kg i.p.) was used as the standard drug.
  
  
  RESULTS
  It was observed that the juice produced significant and dose dependent increase in the time spent in light cubicle (p<0.001), transfer latency from the light to dark cubicle (p<0.001) and the number of transitions between the two cubicles (p<0.001) as compared with the control group. V. vinifera also demonstrated significant and dose dependent increase in ambulation (P<0.001) and rearing (p<0.001) in open field test as compared to the control group.
  
  
  CONCLUSIONS
  In conclusion, the present study establishes the anxiolytic-like activity of VVJ in animal models of anxiety.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Karachi-75270, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Aslam
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Karachi-75270, Pakistan.
authors.forename: Nuzhat
authors.initials: N
authors.lastname: Sultana
keywords: Anxiety; Flavonoids; Open field test; The light/dark box test; Vitis vinifera