title: The secretome of MUSE cells contains factors that may play a role in regulation of stemness, apoptosis and immunomodulation.
abstract: Mesenchymal stromal cells (MSCs) are a heterogeneous population, which contain several cell phenotypes: mesenchymal stem cells, progenitor cells, fibroblasts and other type of cells. Previously, we identified unique stem cells that we named multilineage-differentiating stress enduring (Muse) cells as one to several percent of MSCs of the bone marrow, adipose tissue and dermis. Among different cell populations in MSCs, Muse cells, positive for pluripotent surface marker SSEA-3, may represent cells responsible for pluripotent-like property of MSCs, since they express pluripotency genes, able to differentiated into triploblastic cells from a single cells and are self-renewable. MSCs release biologically active factors that have profound effects on local cellular dynamics. A thorough examination of MSC secretome seems essential for understanding the physiological functions exerted by these cells in our organism and also for rational cellular therapy design. In this setting, studies on secretome of Muse cells may shed light on pathways that are associated with their specific features. Our findings evidenced that secretomes of MSCs and Muse cells contain factors that regulate extracellular matrix remodeling, ox-redox activities and immune system. Muse cells appear to secrete factors that may preserve their stem cell features, allow survival under stress conditions and may contribute to their immunomodulation capacity. In detail, the proteins belonging to protein kinase A signaling, FXR/RXR activation and LXR/RXR activation pathways may play a role in regulation of Muse stem cell features. These last 2 pathways together with proteins associated with antigen presentation pathway and coagulation system may play a role in immunomodulation.
authors.affiliation: a Department of Experimental Medicine , Biotechnology and Molecular Biology Section, Second University of Naples , Naples , Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Alessio
authors.affiliation: b Genome and Stem Cell Center (GENKOK), Erciyes University , Kayseri , Turkey.|c Graduate School of Health Sciences, Erciyes Universty , Kayseri , Turkey.
authors.forename: Servet
authors.initials: S
authors.lastname: Özcan
authors.affiliation: d Department of Stem Cell Biology and Histology , Tohoku University Graduate School of Medicine , Sendai , Japan.|e Tohoku Laboratory Non-clinical Research Division, Clio, Inc. , Sendai , Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Tatsumi
authors.affiliation: c Graduate School of Health Sciences, Erciyes Universty , Kayseri , Turkey.
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Murat
authors.affiliation: f Institute of Bioscience and Bioresources, CNR , Naples , Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Peluso
authors.affiliation: e Tohoku Laboratory Non-clinical Research Division, Clio, Inc. , Sendai , Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Dezawa
authors.affiliation: a Department of Experimental Medicine , Biotechnology and Molecular Biology Section, Second University of Naples , Naples , Italy.|b Genome and Stem Cell Center (GENKOK), Erciyes University , Kayseri , Turkey.|g Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, Temple University , Philadelphia , PA , USA.
authors.forename: Umberto
authors.initials: U
authors.lastname: Galderisi
mesh_terms: D017209:Apoptosis; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D006801:Humans; D056747:Immunomodulation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D055442:Metabolome; D015398:Signal Transduction; D013312:Stress, Physiological
keywords: Mesenchymal stem cells; apoptosis; cancer; secretome; senescence