title: The inflammatory effect of infection with Hymenolepis diminuta via the increased expression and activity of COX-1 and COX-2 in the rat jejunum and colon.
abstract: The aim of this study was to determine whether Hymenolepis diminuta may affect the expression and activity of cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2), resulting in the altered levels of their main products - prostaglandins (PGE2) and thromboxane B2 (TXB2). The study used the same experimental model as in our previous studies in which we had observed changes in the transepithelial ion transport, tight junctions and in the indicators of oxidative stress, in both small and large intestines of rats infected with H. diminuta. In this paper, we investigated not only the site of immediate presence of the tapeworm (jejunum), but also a distant site (colon). Inflammation related to H. diminuta infection is associated with the increased expression and activation of cyclooxygenase (COX), enzyme responsible for the synthesis of PGE2 and TXB2, local hormones contributing to the enhanced inflammatory reaction in the jejunum and colon in the infected rats. The increased COX expression and activity is probably caused by the increased levels of free radicals and the weakening of the host's antioxidant defense induced by the presence of the parasite. Our immunohistochemical analysis showed that H. diminuta infection affected not only the intensity of the immunodetection of COX but also the enzyme protein localization within intestinal epithelial cells - from the entire cytoplasm to apical/basal regions of cells, or even to the nucleus.
authors.affiliation: Department of Biology and Medical Parasitology, Pomeranian Medical University, Szczecin, Poland. Electronic address: kodan@pum.edu.pl.
authors.forename: D I
authors.initials: DI
authors.lastname: Kosik-Bogacka
authors.affiliation: Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Baranowska-Bosiacka
authors.affiliation: Department of Histology and Embryology, Pomeranian Medical University, Szczecin, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Kolasa-Wołosiuk
authors.affiliation: Department of Biology and Medical Parasitology, Pomeranian Medical University, Szczecin, Poland.
authors.forename: N
authors.initials: N
authors.lastname: Lanocha-Arendarczyk
authors.affiliation: Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Gutowska
authors.affiliation: Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Korbecki
authors.affiliation: Department of Biology and Medical Parasitology, Pomeranian Medical University, Szczecin, Poland.
authors.forename: H
authors.initials: H
authors.lastname: Namięta
authors.affiliation: Independent Laboratory of Medical Rehabilitation, Pomeranian Medical University, Szczecin, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Rotter
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003106:Colon / Q000201:enzymology* / Q000469:parasitology; D051545:Cyclooxygenase 1 / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism; D006925:Hymenolepiasis / Q000201:enzymology* / Q000469:parasitology / Q000473:pathology; D048190:Hymenolepis diminuta / Q000502:physiology*; D007150:Immunohistochemistry; D007249:Inflammation; D007583:Jejunum / Q000201:enzymology* / Q000469:parasitology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013929:Thromboxane B2 / Q000378:metabolism; D014231:Tribolium
chemical_list: D013929:Thromboxane B2; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D015232:Dinoprostone
keywords: Cyclooxygenase 1 (COX-1); Cyclooxygenase 2 (COX-2); Hymenolepis diminuta; Prostaglandin E(2) (PGE(2)); Thromboxane B(2) (TXB(2))