title: The expression of inhibitor of growth 4 is reduced in cervical cancer tissues.
abstract: Inhibitor of growth 4 (ING4) is a candidate tumor suppressor which plays an important role in multiple processes including DNA repair, apoptosis, cell cycle control, tumor metastasis and angiogenesis. However, clinical data about the role of ING4 in the development and progression of cervical cancer are still limited. This study aimed to examine ING4 expression in cervical cancer and analyze its correlation with the progression of the malignancy.
  
  
  
  RT-PCR, Western blot and immunohistochemistry analysis were performed to determine ING4 expression in 18 clinical specimens from cervical cancer patients. The correlation of ING4 expression with the clinical-pathological features of the patients was analyzed. Moreover, the correlation between ING4 and HPV E6/E7 transcription level in SiHa cells was analyzed.
  
  
  
  ING4 expression was decreased significantly at mRNA and protein levels in the tissues of cervical cancer compared with paracarcinoma tissues. Analysis of the subcellular localization of ING4 showed that ING4 expression was decreased in the nucleus of cervical cancer tissues. Ectopic expression of ING4 reduced the proliferation of SiHa cells, accompanied by decreased HPV E6/E7 transcription.
  
  
  
  ING4 expression is decreased in human cervical cancer tissues. Reconstitution of ING4 expression in cervical cancer cells is correlated with decreased HPV E6/E7 transcription. These data suggest that ING4 expression has diagnostic and prognostic significance for cervical cancer.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China. wangyan@hrbmu.edu.cn.
authors.forename: J
authors.initials: J
authors.lastname: Yuan
authors.forename: Y-L
authors.initials: YL
authors.lastname: Han
authors.forename: T-Y
authors.initials: TY
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Wu
authors.forename: L
authors.initials: L
authors.lastname: Tong
authors.forename: L-X
authors.initials: LX
authors.lastname: Lin
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: L-L
authors.initials: LL
authors.lastname: Wei
authors.forename: Z-H
authors.initials: ZH
authors.lastname: Zhong
authors.forename: G-M
authors.initials: GM
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D018797:Cell Cycle Proteins; D005260:Female; D018398:Homeodomain Proteins; D006801:Humans; D007150:Immunohistochemistry; D009856:Oncogene Proteins, Viral / Q000378:metabolism; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D018398:Homeodomain Proteins; C483230:ING4 protein, human; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins