title: Impact of poplar-based phytomanagement on soil properties and microbial communities in a metal-contaminated site.
abstract: Despite a long history of use in phytomanagement strategies, the impacts of poplar trees on the structure and function of microbial communities that live in the soil remain largely unknown. The current study combined fungal and bacterial community analyses from different management regimes using Illumina-based sequencing with soil analysis. The poplar phytomanagement regimes led to a significant increase in soil fertility and a decreased bioavailability of Zn and Cd, in concert with changes in the microbial communities. The most notable changes in the relative abundance of taxa and operational taxonomic units unsurprisingly indicated that root and soil constitute distinct ecological microbial habitats, as exemplified by the dominance of Laccaria in root samples. The poplar cultivar was also an important driver, explaining 12% and 6% of the variance in the fungal and bacterial data sets, respectively. The overall dominance of saprophytic fungi, e.g. Penicillium canescens, might be related to the decomposition activities needed at the experimental site. Our data further highlighted that the mycorrhizal colonization of poplar cultivars varies greatly between the species and genotypes, which is exemplified by the dominance of Scleroderma under Vesten samples. Further interactions between fungal and bacterial functional groups stressed the potential of high-throughput sequencing technologies in uncovering the microbial ecology of disturbed environments.
authors.affiliation: Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Foulon
authors.affiliation: Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Zappelini
authors.affiliation: Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Durand
authors.affiliation: Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Valot
authors.affiliation: CNRS, LIEC UMR7360, Faculté des Sciences et Technologies, BP70239, 54506 Vandoeuvre-lès-Nancy, France Université de Lorraine, LIEC UMR7360, Faculté des Sciences et Technologies, BP 70239, 54506 Vandoeuvre-lès-Nancy, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Blaudez
authors.affiliation: Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France Université de Lorraine, Faculté des Sciences et Technologies, BP 70239, 54506 Vandoeuvre-les-Nancy, France michel.chalot@univ-fcomte.fr.
authors.forename: Michel
authors.initials: M
authors.lastname: Chalot
mesh_terms: D001673:Biodegradation, Environmental; D017753:Ecosystem; D004777:Environment; D059014:High-Throughput Nucleotide Sequencing; D008670:Metals / Q000032:analysis* / Q000378:metabolism; D059013:Microbial Consortia; D038821:Mycorrhizae; D018517:Plant Roots / Q000382:microbiology; D032107:Populus; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
chemical_list: D008670:Metals; D012987:Soil; D012989:Soil Pollutants
keywords: Illumina-based sequencing; bacterial 16S; fungal ITS; metal-contaminated soils; phytomanagement, poplar cultivars