title: MiR-433 inhibits retinoblastoma malignancy by suppressing Notch1 and PAX6 expression.
abstract: Retinoblastoma (RB) is the most frequent primary intraocular cancer. It has been demonstrated by previous studies that retinoblastoma is initiated primarily by the inactivation of the retinoblastoma Rb1 gene in retinal cells. However, additional genetic alterations than Rb1 mutation could play important roles in the process of transforming benign retinal cells into retinoblastoma tumor cells. In this study, we identified that microRNA miR-433 is one of such genetic factors. We found that the expression levels of miR-433 were downregulated in RB tissues. We also determined that miR-433 negatively regulated RB cell proliferation, migration and invasion, and induced cell cycle arrest and apoptosis of RB cells. We used bioinformatics method to predict and confirmed that Notch1 and PAX6 were miR-433 target genes in RB cells. Importantly, we demonstrated that restoration of Notch1 and PAX6 expression partially rescued the inhibition of cell proliferation and metastasis induced by miR-433 overexpression, suggesting that miR-433 regulates RB cell proliferation and metastasis through suppressing the expression of Notch1 and PAX6.
authors.affiliation: Department of Ophthalmology, Hongqi Hospital of Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Hongqi Hospital of Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Ophthalmology, Hongqi Hospital of Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, China.
authors.forename: Tianjiao
authors.initials: T
authors.lastname: Shuai
authors.affiliation: Department of Ophthalmology, Hongqi Hospital of Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, China.
authors.forename: Tianhua
authors.initials: T
authors.lastname: Piao
authors.affiliation: Department of Ophthalmology, Hongqi Hospital of Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, China. Electronic address: wangrui214214@163.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D000071841:PAX6 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism*; D012175:Retinoblastoma / Q000235:genetics* / Q000473:pathology*
chemical_list: C548883:MIRN433 microRNA, human; D035683:MicroRNAs; D000071841:PAX6 Transcription Factor; C000605730:PAX6 protein, human; D051880:Receptors, Notch
keywords: MiR-433; Notch1; PAX6; Retinoblastoma