title: Sphingomonas prati sp. nov., isolated from alpine meadow soil.
abstract: A Gram-staining-negative, non-motile, orange-coloured and rod-shaped aerobic bacterium, designated W18RDT, was isolated from the alpine meadow soil of the Tibetan plateau. Phylogenetic analysis based on 16S rRNA gene sequences positioned strain W18RDT as a representative of a novel species under the genus Sphingomonas which was most closely related to Sphingomonas fennica DSM 13665T with a sequence similarity level of 97.14 %. Meanwhile, it also had a high level of sequence similarity with Sphingomonas laterariae DSM 25432T (96.51 %), Sphingomonas haloaromaticamans CGMCC 1.10206T (96.43 %) and Sphingomonas formosensis DSM 24164T (96.26 %). The G+C content of the genomic DNA of the type strain W18RDT was 66.4mol%. DNA-DNA relatedness for the type strain W18RDT with respect to its closest phylogenetic relative Sphingomonas. fennica DSM 13665Twas 21.54±1.2 %. Major cellular fatty acids in strain W18RDT were C16 : 1ω7c and/or C16 : 1ω6c (48.12 %), C18 : 1ω7c and/or C18 : 1ω6c (21.98 %) and C14 : 0 2-OH (14.93 %), with ubiquinone-10 (Q-10) as the predominant respiratory quinone. The polar lipid profile of the strain consisted of phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, diphosphatidylglycerol and two unknown lipids. Based on the evidence from a combination of phenotypic, taxonomic and phylogenetic analyses, strain W18RDT represents a novel species of the genus Sphingomonas, for which the name Sphingomonas prati sp. nov. is proposed. The type strain is W18RDT (=CGMCC 1.15645T=DSM 103336T).
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, PR China.|University of Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Pooja
authors.initials: P
authors.lastname: Manandhar
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Gengxin
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, PR China.|University of Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Yilun
authors.initials: Y
authors.lastname: Hu
authors.affiliation: School of Environment, Tsinghua University, Beijing, PR China.
authors.forename: Arun
authors.initials: A
authors.lastname: Lama
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, PR China.|Hebei University of Engineering, Handan, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: University of Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Zhengquan
authors.initials: Z
authors.lastname: Gu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D065948:Grassland; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D020578:Sphingomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018609:Tibet; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2