title: Development of a new antibacterial biomaterial by tetracycline immobilization on calcium-alginate beads.
abstract: In recent years, increasing risk of infection, caused by resistant microorganism to antibiotics, has become the limelight discovery of new and natural antibacterial materials. Heavy metals, such as silver, copper, mercury and titanium, have antibacterial activity. Products, which improved these metals, do not have stable antibacterial property. Therefore, use of these products is restricted. The aim of this study was to immobilize tetracycline to alginate and improve an antibacterial biomaterial. For this purpose, calcium-alginate beads were formed by dropping to calcium-chloride solution and tetracycline was immobilized to beads using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide at optimum conditions. After immobilization, actualization of immobilization was investigated by analyzing ATR-FTIR spectrum and SEM images. Also, antibacterial property of obtained product was tested. Improved product demonstrated antibacterial property. It has potential for open wound, surgical drapes, bed and pillow sheath in hospitals and it may also be used for increasing human comfort in daily life.
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, Bornova, Izmir 35100, Turkey.
authors.forename: Emine Erdogan
authors.initials: EE
authors.lastname: Ozseker
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, Bornova, Izmir 35100, Turkey. Electronic address: alp212000@gmail.com.
authors.forename: Alper
authors.initials: A
authors.lastname: Akkaya
mesh_terms: D000464:Alginates / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001458:Bandages; D001512:Bedding and Linens; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002118:Calcium / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013752:Tetracycline / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D013752:Tetracycline; D005022:Ethyldimethylaminopropyl Carbodiimide; D002118:Calcium
keywords: 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide; Antibacterial biomaterial; Calcium-alginate beads; Immobilization; Tetracycline