title: Effects of a Small Interfering RNA Targeting YKL-40 Gene on the Proliferation and Invasion of Endometrial Cancer HEC-1A Cells.
abstract: The purpose of this study was to explore the effects of a small interfering RNA (siRNA) targeting YKL-40 on the proliferation and invasion of endometrial cancer (EC) HEC-1A cells.
  
  
  
  We used an siRNA targeting a sequence in YKL-40 (si-YKL-40) to transfect HEC-1A cells. Quantitative real-time polymerase chain reaction assay was performed to investigate the mRNA levels of YKL-40. MTT, migration, and invasion assays were performed to identify the effects of si-YKL-40 on the proliferation, migration, and invasive abilities of the HEC-1A cells.
  
  
  
  mRNA expression of YKL-40 was down-regulated in HEC-1A cells after transfection with si-YKL-40 (P < 0.05). The proliferation, migration, and invasive abilities of HEC-1A cells were inhibited by siRNA (P < 0.05).
  
  
  
  YKL-40 targeting siRNA specifically blocks the activity of YKL-40 in human EC HEC-1A cells, resulting in tumor suppression. This indicates that YKL-40 might serve as a potential small molecule target in the treatment of EC.
authors.affiliation: *Department of Gynecology, First Affiliated Hospital of Guangxi Medical University, Nanning; and †Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Li
authors.forename: Jiang-Tao
authors.initials: JT
authors.lastname: Fan
authors.forename: Da-Hai
authors.initials: DH
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D054443:Cell Migration Assays; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D000071451:Chitinase-3-Like Protein 1 / Q000235:genetics*; D004354:Drug Screening Assays, Antitumor; D016889:Endometrial Neoplasms / Q000188:drug therapy*; D005260:Female; D006801:Humans; D034741:RNA, Small Interfering / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1; D034741:RNA, Small Interfering