title: Sickle Cell Imaging Flow Cytometry Assay (SIFCA).
abstract: Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a partially automated, high-throughput sickling assay using imaging flow cytometry.
authors.affiliation: Department of Clinical Pathology, School of Medical Sciences, University of Campinas, Campinas, Brazil.|Hematology and Hemotherapy Center-Hemocentro Campinas, University of Campinas, Campinas, Brazil.
authors.forename: Kleber Y
authors.initials: KY
authors.lastname: Fertrin
authors.affiliation: NHLBI Flow Cytometry Core Facility, NHLBI, NIH, Building 31, Room 5A52, MSC 2486, 31 Center Drive, Bethesda, MD, 20892, USA.
authors.forename: Leigh
authors.initials: L
authors.lastname: Samsel
authors.affiliation: Department of Clinical Hematology, Academic Medical Centre, Amsterdam, The Netherlands.
authors.forename: Eduard J
authors.initials: EJ
authors.lastname: van Beers
authors.affiliation: Hematology Branch, NHLBI, NIH, Bethesda, MD, 20892, USA.
authors.forename: Laurel
authors.initials: L
authors.lastname: Mendelsohn
authors.affiliation: Division of Hematology/Oncology, Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, 15261, USA.
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Kato
authors.affiliation: NHLBI Flow Cytometry Core Facility, NHLBI, NIH, Building 31, Room 5A52, MSC 2486, 31 Center Drive, Bethesda, MD, 20892, USA. mccoyj@nhlbi.nih.go.
authors.forename: J Philip
authors.initials: JP
authors.lastname: McCoy
mesh_terms: D000755:Anemia, Sickle Cell / Q000473:pathology*; D004912:Erythrocytes / Q000473:pathology*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006451:Hemoglobin, Sickle / Q000032:analysis; D006801:Humans
chemical_list: D006451:Hemoglobin, Sickle
keywords: Imaging flow cytometry; Sickle cell disease; Sickling assay