title: Formation of RNA Beads from Various DNA Nanoring Structures.
abstract: By taking advantages of rolling circle transcription, one of the powerful methods to overcome the instability of RNA, we successfully synthesized RNA particles having high copy numbers of RNA strands. To examine dependence of RNA particle formation to template circular DNA strands, we synthesized RNA beads with 65-nt, 92-nt and 200-nt DNA nanoring structures as template circular DNA. Our conclusion is that characteristics of RNA beads could be controlled with various template circular DNA for RCT. It is feasible that our RNA beads could be used for RNAzyme-based metal sensors such as aqueous lead ion sensing. In this case, we expect that multi-metal ion detection would be possible by using 200-nt circular DNA bearing multiple desired functions as a template for RNA bead formation. Furthermore, certain features of RNA beads such as sensitivity to nuclease digestion and maximum loading amount of drugs when used as a carrier are expected to be further adjusted by choosing appropriate porosity and size.
authors.forename: Hyejin
authors.initials: H
authors.lastname: Kim
authors.forename: Jong Bum
authors.initials: JB
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D003460:Crystallization / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry; D008422:Materials Testing; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D012313:RNA / Q000138:chemical synthesis* / Q000648:ultrastructure*; D014764:Viral Proteins / Q000737:chemistry
chemical_list: D014764:Viral Proteins; D012313:RNA; D004247:DNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases