title: Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics.
abstract: We present a high-throughput microfluidics platform to identify novel host cell binding partners of respiratory syncytial virus (RSV) matrix (M) protein. The device consists of thousands of reaction chambers controlled by micro-mechanical valves. The microfluidic device is mated to a microarray-printed custom-made gene library. These genes are then transcribed and translated on-chip, resulting in a protein array ready for binding to RSV M protein.Even small viral proteome, such as that of RSV, presents a challenge due to the fact that viral proteins are usually multifunctional and thus their interaction with the host is complex. Protein microarrays technology allows the interrogation of protein-protein interactions, which could possibly overcome obstacles by using conventional high throughput methods. Using microfluidics platform we have identified new host interactors of M involved in various cellular pathways. A number of microfluidics based assays have already provided novel insights into the virus-host interactome, and the results have important implications for future antiviral strategies aimed at targets of viral protein interactions with the host.
authors.affiliation: Nanotechnology Institute, Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel.
authors.forename: Sarit
authors.initials: S
authors.lastname: Kipper
authors.affiliation: Nanotechnology Institute, Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel.
authors.forename: Dorit
authors.initials: D
authors.lastname: Avrahami
authors.affiliation: Section of Virology, Faculty of Medicine, Imperial College London, St. Mary's Campus, Norfolk Place, London, W2 UK, UK. mbajorek@jouy.inra.fr.
authors.forename: Monika
authors.initials: M
authors.lastname: Bajorek
authors.affiliation: Nanotechnology Institute, Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel. Doron.Gerber@biu.ac.il.
authors.forename: Doron
authors.initials: D
authors.lastname: Gerber
mesh_terms: D015723:Gene Library; D057809:HEK293 Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D066330:Printing, Three-Dimensional; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods; D025941:Protein Interaction Mapping / Q000295:instrumentation / Q000379:methods*; D018357:Respiratory Syncytial Virus Infections / Q000378:metabolism* / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000378:metabolism / Q000502:physiology*; D014763:Viral Matrix Proteins / Q000378:metabolism*
chemical_list: D014763:Viral Matrix Proteins
keywords: Integrated microfluidics; Protein arrays; RSV–host interaction; Virus–host interactions