title: Novel diagnostic biomarker for patients with Non-Hodgkin's Lymphoma by IgH gene rearrangement.
abstract: OBJECTIVE
  Novel biomarkers for improving accuracy could be beneficial for disease monitoring and surveillance of Non-Hodgkin's lymphomas (NHL). So we explored the viability of analytical methods for identifying the rearranged immunoglobulin (Ig) H genes sequence.
  
  
  METHODS
  Next-generation sequencing (NGS) was used to sequence deoxyribonucleic acid (DNA) extracted directly from the tumor tissues of patients with NHL, and then specific rearranged DNA fragments from plasma was detected by polymerase chain reaction (PCR).
  
  
  RESULTS
  By parallel DNA capturing and sequencing of IgH genomic regions (IgCap), the sequence of rearranged IgH loci could be detected and precisely determined in tumor tissues of 12 patients with NHL. The circulating rearranged DNA fragments had been identified in the plasma of one patient.
  
  
  CONCLUSIONS
  IgCap may be the favorable diagnostic method for patients with NHL in clinical.
authors.affiliation: Department of Hematology, Chinese PLA (People's Liberation Army) General Hospital, Beijing 100853, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Bo
authors.affiliation: Departments of Pathology, Chinese PLA (People's Liberation Army) General Hospital, Beijing 100853, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Hematology, Tangdu Hospital, Fourth Military Medical University, Xian 710038, China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Hematology, Chinese PLA (People's Liberation Army) General Hospital, Beijing 100853, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Hematology, Chinese PLA (People's Liberation Army) General Hospital, Beijing 100853, China.
authors.forename: Honghua
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Hematology, Chinese PLA (People's Liberation Army) General Hospital, Beijing 100853, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D001483:Base Sequence; D014408:Biomarkers, Tumor; D019295:Computational Biology / Q000379:methods; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D007143:Immunoglobulin Heavy Chains