title: The role of porcine reproductive and respiratory syndrome virus infection in immune phenotype and Th1/Th2 balance of dendritic cells.
abstract: The aim of this study was to characterize the immune response of dendritic cells derived from monocytes (Mo-DCs) in the porcine peripheral blood following infection with porcine reproductive and respiratory syndrome virus (PRRSV). Viral load assays indicated that PRRSV efficiently infected Mo-DCs but failed to replicate, whereas PRRSV infection of Mo-DCs decreased the expression of SLA-I, SLA-II, CD80 and CD40 compared with those of mock Mo-DCs. Furthermore, we analyzed the cytokine profiles using quantitative RT-PCR and ELISA. Results indicated apparent changes in IL-10 and IL-12 p40 expression but not in IFN-γ and TNF-α among Mo-DCs infected with PRRSV and uninfected Mo-DCs. Additionally, flow cytometry analysis of the altered Mo-DCs together with IL-4 and GM-CSF induction for 7days revealed the typical morphology and phenotype with 91.73% purity before infection with PRRSV. Overall, our data demonstrate that PRRSV impaired the normal antigen presentation of Mo-DCs and led to inadequate adaptive immune response by down-regulating the expression of SLA-I,SLA-II, CD80 and CD40. Enhanced Th2 -type cytokine IL-10 secretion and reduced Th1-type cytokines IL-12p40,IFN-γ and TNF-α secretion results in Th1/Th2 imbalance.
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, PR China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Liu
authors.affiliation: The Preventive Center of Animal Disease of LiaoNing Province, No.95, Renhe Road, Shenbei District, Shenyang 110164, PR China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wei
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, PR China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Immunology, Basic School of Medicine, China Medical University, No. 92, North Second Road, Shenyang 110001, PR China.
authors.forename: Fengping
authors.initials: F
authors.lastname: Shan
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, PR China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, PR China. Electronic address: Shenguoshun@sohu.com.
authors.forename: Guoshun
authors.initials: G
authors.lastname: Shen
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D017951:Antigen Presentation; D018018:Arterivirus / Q000502:physiology*; D018174:Arterivirus Infections / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D006649:Histocompatibility Antigens / Q000378:metabolism; D057131:Immune Evasion; D009000:Monocytes / Q000276:immunology*; D013552:Swine; D058408:Th1-Th2 Balance; D059467:Transcriptome; D019562:Viral Load
chemical_list: D016207:Cytokines; D006649:Histocompatibility Antigens
keywords: Dendritic cell; Immunophenotype; PRRSV; Thl /Th2