title: Establishment of reference intervals of thyroid function tests from cord blood of neonates in two selected hospitals, Addis Ababa, Ethiopia.
abstract: Reference intervals are affected by different factors such as lifestyle, ethnicity, age/developmental stage, gender, nutrition and other environmental factors (Clin Biochem Rev: 29,2008). Therefore, it is obvious that it should be established for every population in different regions even within a country. Then the aim of this study is to establish population specific reference intervals of thyroid stimulating hormone, free thyroxine and free triidothyronine levels of cord blood.
  
  
  
  One hundred twenty three cord blood samples collected from the umbilical cord of newborns were analyzed for thyroid stimulating hormone, free thyroxine and free triidothyronine values. The birth weights ranged between 2500 and 4700 g with mean (SD) value of 3241.46 (459.495) gram. Their gestational age ranged between 37 and 44 weeks with an average of 39.74 weeks. The 2.5(th) and 97.5(th) percentiles of values were found to be 3.48 mIU/L and 27.57 mIU/L for thyroid stimulating hormone, 0.89 ng/dl and 1.53 ng/dl for free thyroxine and 1.19 pg/ml and 2.51 pg/ml for free triidothyronine respectively.
  
  
  
  In the present study the reference intervals of thyroid stimulating hormone, free thyroxine and free triidothyronine were established and based on the results obtained, were 3.48-27.56 mIU/L for thyroid stimulating hormone, 0.89-1.53 ng/dl for free thyroxine and 1.19-2.51 pg/ml for free triidothyronine. It has been concluded that the result can provide us with an important baseline to establish population specific reference intervals for our country using large scale studies.
authors.affiliation: Department of Medical Biochemistry and Molecular Biology, Biomedical Institute, College of Health Sciences, Mekelle University, Mekelle, Ethiopia. amanmehari71@gmail.com.
authors.forename: Aman
authors.initials: A
authors.lastname: Mehari
authors.affiliation: Clinical Chemistry Department, Directorate of TB & HIV research, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
authors.forename: Feyssa
authors.initials: F
authors.lastname: Challa
authors.affiliation: Department of Pediatrics, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
authors.forename: Goitom
authors.initials: G
authors.lastname: Gebreyesus
authors.affiliation: Department of Pediatrics, Gandhi Memorial Hospital, Addis Ababa, Ethiopia.
authors.forename: Dereje
authors.initials: D
authors.lastname: Alemayehu
authors.affiliation: Department of Biochemistry, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Seifu
mesh_terms: D001724:Birth Weight; D003409:Congenital Hypothyroidism / Q000175:diagnosis; D003430:Cross-Sectional Studies; D005002:Ethiopia; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D015997:Neonatal Screening / Q000592:standards*; D012016:Reference Values; D013960:Thyroid Function Tests / Q000592:standards*; D013972:Thyrotropin / Q000097:blood*; D013974:Thyroxine / Q000097:blood*; D014284:Triiodothyronine / Q000097:blood*
chemical_list: D014284:Triiodothyronine; D013972:Thyrotropin; D013974:Thyroxine
keywords: Congenital hypothyroidism; Reference intervals; Screening program; Thyroid function tests