title: Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China.
abstract: A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1(T), was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1(T) grew optimally at pH 7.0-8.0, at 28-30 °C and in the presence of NaCl concentrations from 0.0% to 0.5 %. 16S rRNA gene sequence analysis indicated that strain 25-1(T) belongs to the genus Chryseobacterium within the family Flavobacteriaceae and is related most closely to C. carnis G81(T) (96.4% similarity), C. lathyri RBA2-6(T) (95.8% similarity), and C. zeae JM1085(T) (95.8% similarity). Its genomic DNA G+C molar composition was 36.2%. The major cellular fatty acids were iso-C15:0 (44.0%), iso-C17:0 3OH (19.8%) and C16:1 ω7c/16:1 ω6c (12.7%). The only isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA-DNA relatedness between strain 25-1(T) and C. lathyri RBA2-6(T) was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1(T) is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1(T) (CCTCC AB2015133(T)=DSM 100396(T)).
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Cai-Fang
authors.initials: CF
authors.lastname: Wen
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Xi
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Zhong-Xiang
authors.initials: ZX
authors.lastname: Hao
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Luo
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liao
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Zhen-Rong
authors.initials: ZR
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Rong
authors.initials: R
authors.lastname: She
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
authors.forename: Guo-Quan
authors.initials: GQ
authors.lastname: Han
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: San-Jie
authors.initials: SJ
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Qi-Gui
authors.initials: QG
authors.lastname: Yan
authors.affiliation: Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Hou
mesh_terms: D000818:Animals; D002681:China; D045247:Chryseobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D001503:Ursidae / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA sequencing; Chryseobacterium chengduensis; Giant panda; Phylogenetic analysis; Strain 25-1T