title: Identification and Characterization of Salvia miltiorrhizain miRNAs in Response to Replanting Disease.
abstract: Replanting disease is a major factor limiting the artificial cultivation of the traditional Chinese medicinal herb Salvia miltiorrhiza. At present, little information is available regarding the role of miRNAs in response to replanting disease. In this study, two small RNA libraries obtained from first-year (FPR) and second-year plant (SPR) roots were subjected to a high-throughput sequencing method. Bioinformatics analysis revealed that 110 known and 7 novel miRNAs were annotated in the roots of S. miltiorrhiza. Moreover, 39 known and 2 novel miRNAs were identified and validated for differential expression in FPR compared with SPR. Thirty-one of these miRNAs were further analyzed by qRT-PCR, which revealed that 5 miRNAs negatively regulated the expression levels of 7 target genes involved in root development or stress responses. This study not only provides novel insights into the miRNA content of S. miltiorrhiza in response to replanting disease but also demonstrates that 5 miRNAs may be involved in these responses. Interactions among the differentially expressed miRNAs with their targets may form an important component of the molecular basis of replanting disease in S. miltiorrhiza.
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, 712100, China.|College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Haihua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Weibo
authors.initials: W
authors.lastname: Jin
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, 712100, China.
authors.forename: Xiaole
authors.initials: X
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, 712100, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, 712100, China.
authors.forename: Zhigui
authors.initials: Z
authors.lastname: He
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, 712100, China.
authors.forename: Shushen
authors.initials: S
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, 712100, China.|College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Zongsuo
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Tasly R&D Institute, Tasly Holding Group Co. Ltd, Tianjin, 300410, China.
authors.forename: Xijun
authors.initials: X
authors.lastname: Yan
authors.affiliation: Tasly R&D Institute, Tasly Holding Group Co. Ltd, Tianjin, 300410, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: He
authors.affiliation: Tianjin Tasly Modern TCM Resources Co., Ltd., Tianjin, 300402, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics*; D000069600:Plant Breeding; D010935:Plant Diseases / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D018749:RNA, Plant / Q000235:genetics*; D027561:Salvia miltiorrhiza / Q000235:genetics* / Q000254:growth & development*; D017423:Sequence Analysis, RNA
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant