title: A dual-channel fluorescent chemosensor for discriminative detection of glutathione based on functionalized carbon quantum dots.
abstract: A convenient, fluorescent dual-channel chemosensor on the basis of bis(3-pyridylmethyl)amine-functionalized carbon quantum dots (BPMA-CQDs) nanoprobe was constructed, and it can discriminatively detect glutathione from its analogues cysteine and homocysteine based on two distinctive strategies. Two distinct fluorescence responses of BPMA-CQDs probe to Cu(II) and Ag(I) were identified and further employed to achieve selective detection of Cu(II) and Ag(I) respectively. Based on the BPMA-CQDs/Cu(II) conjugate, discriminative detection of GSH was achieved in terms of correlation between the amounts of GSH and fluorescence recovery. The addition of GSH into BPMA-CQDs/Cu(II) system induces the reduction of Cu(II) to Cu(I), which could efficiently block PET process resulting in the following fluorescence recovery. Based on the BPMA-CQDs/Ag(I) conjugate, GSH assay could also be established on the basis of fluorescence response to GSH. The introduction of GSH into the preceding system triggers the competitive coordination to Ag(I) between BPMA and GSH, and silver ions are finally taken away by GSH from the probe, where the fluorescence is restored to its original weak state. Both of the detection strategies can achieve discriminative detection of GSH from Cys and Hcy. The assays showed good stability and repeatability, and covered a broad linear range of up to 13.3μM with a lowest detection limit of 42.0nM. Moreover, both of them were utilized to monitor GSH level in live cells.
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Feng
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Jieyu
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Ma
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Tang
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Ao
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Meizhi
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China. Electronic address: qianzhaosheng@zjnu.cn.
authors.forename: Zhaosheng
authors.initials: Z
authors.lastname: Qian
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry; D005978:Glutathione / Q000032:analysis* / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002244:Carbon; D005978:Glutathione
keywords: Carbon quantum dots; Chemosensor; Fluorometric assay; Glutathione detection