title: Surface modification of SOI-FET sensors for label-free and specific detection of short RNA analyte.
abstract: A new type of surface modification of SOI-FET sensors with ultrathin sensor-probe transition layer and uncharged probes for highly specific detection of short RNA (saRNA) was suggested.
  
  
  
  Carbonyldiimidazole (CDI) or glycidoxypropyltrimethoxysilane were used as precursors of sensor-probe interface layers, together with peptide nucleic acids and new NA analogues - phosphoryl guanidine oligo(2'-OMe)ribonucleotides (PGO) as probes for RNA hybridization. RNA sequences corresponding to mRNA NELFA (NM_005663) and microRNA-29a (cancer markers) were used as saRNA targets. Real-time saRNA detection by SOI-FET sensors and fluorescence analysis were applied.
  
  
  
  Highly specific response with femtomolar sensitivity to saRNA was demonstrated for CDI-PGO-modified sensors fabricated by optical lithography.
  
  
  
  The proposed CDI-PGO protocol of modification of Si sensor surface is a promising procedure for biomedical applications.
authors.affiliation: SB RAS, Institute of Chemical Biology & Fundamental Medicine, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Dmitrienko
authors.affiliation: SB RAS, A.V. Rzhanov Institute of Semiconductor Physics, Novosibirsk, 13 Lavrentiev Avenue 630090, Russia.
authors.forename: Olga
authors.initials: O
authors.lastname: Naumova
authors.affiliation: SB RAS, A.V. Rzhanov Institute of Semiconductor Physics, Novosibirsk, 13 Lavrentiev Avenue 630090, Russia.
authors.forename: Boris
authors.initials: B
authors.lastname: Fomin
authors.affiliation: SB RAS, Institute of Chemical Biology & Fundamental Medicine, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia.
authors.forename: Maxim
authors.initials: M
authors.lastname: Kupryushkin
authors.affiliation: SB RAS, Institute of Chemical Biology & Fundamental Medicine, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia.
authors.forename: Alena
authors.initials: A
authors.lastname: Volkova
authors.affiliation: SB RAS, Institute of Chemical Biology & Fundamental Medicine, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia.
authors.forename: Nariman
authors.initials: N
authors.lastname: Amirkhanov
authors.affiliation: SB RAS, Institute of Chemical Biology & Fundamental Medicine, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Semenov
authors.affiliation: SB RAS, Institute of Chemical Biology & Fundamental Medicine, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia.
authors.forename: Inna
authors.initials: I
authors.lastname: Pyshnaya
authors.affiliation: SB RAS, Institute of Chemical Biology & Fundamental Medicine, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia.
authors.forename: Dmitrii
authors.initials: D
authors.lastname: Pyshnyi
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D007093:Imidazoles / Q000737:chemistry; D035683:MicroRNAs / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000032:analysis* / Q000235:genetics; D012821:Silanes / Q000737:chemistry; D013499:Surface Properties; D035602:Transcriptional Elongation Factors / Q000235:genetics
chemical_list: C403136:3-glycidoxypropyltrimethoxysilane; D007093:Imidazoles; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; C120899:NELFA protein, human; D015345:Oligonucleotide Probes; D020135:Peptide Nucleic Acids; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012821:Silanes; D035602:Transcriptional Elongation Factors
keywords: SOI-FET biosensor; short RNA analyte; surface modification