title: Zinc asparaginate supplementation induces redistribution of toxic trace elements in rat tissues and organs.
abstract: The primary objective of the current study was the investigation of the influence of zinc asparaginate supplementation for 7 and 14 days on toxic metal and metalloid content in rat organs and tissues. Rats obtained zinc asparaginate in doses of 5 and 15 mg/kg/day for 7 and 14 days. At the end of the experiment rat tissues and organs (liver, kidney, heart, m. gastrocnemius, serum, and hair) were collected for subsequent analysis. Estimation of Zn, Al, As, Li, Ni, Sn, Sr content in the harvested organs was performed using inductively coupled plasma mass spectrometry at NexION 300D. The obtained data showed that intragastric administration of zinc significantly increased liver, kidney and serum zinc concentrations. Seven-day zinc treatment significantly affected the toxic trace element content in the animals' organs. Zinc supplementation significantly decreased particularly liver aluminium, nickel, and tin content, whereas lead tended to increase. Zinc-induced changes in kidney metal content were characterized by elevated lithium and decreased nickel concentration. Zinc-induced alteration of myocardical toxic element content was multidirectional. Muscle aluminium and lead concentration were reduced in response to zinc supplementation. At the same time, serum and hair toxic element concentrations remained relatively stable after 7-day zinc treatment. Zinc asparaginate treatment of 14 days significantly depressed liver and elevated kidney lithium content, whereas a significant zinc-associated decrease was detected in kidney strontium content. Zinc supplementation for 14 days resulted also in multidirectional changes in the content of heart toxic elements. At the same time, significant zinc-associated decrease in muscle lithium and nickel levels was observed. Fourteen-day zinc treatment resulted in significantly increased serum arsenic and tin concentrations, whereas hair trace element content remained relatively stable. Generally, the obtained data indicate a significant redistribution of toxic metals in the animal organism under zinc supplementation.
authors.affiliation: Federal State Scientific Institution "Institute of Toxicology", Federal Medico-Biological Agency, Bekhtereva str. 1, St. Petersburg 192019, Russia; Russian Society of Trace Elements in Medicine, ANO "Centre for Biotic Medicine", Zemlyanoy Val St. 46, Moscow 105064, Russia.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Skalny
authors.affiliation: Russian Society of Trace Elements in Medicine, ANO "Centre for Biotic Medicine", Zemlyanoy Val St. 46, Moscow 105064, Russia; Laboratory of Biotechnology and Applied Bioelementology, Yaroslavl State University, Sovetskaya st., 14, Yaroslavl, 150000, Russia; Department of Biochemistry, Orenburg State Medical University, Sovetskaya St., 6, Orenburg, 460000, Russia.
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Tinkov
authors.affiliation: Laboratory of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Baltiyskaya St., 8, Moscow, 125315, Russia.
authors.forename: Yulia S
authors.initials: YS
authors.lastname: Medvedeva
authors.affiliation: Laboratory of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Baltiyskaya St., 8, Moscow, 125315, Russia.
authors.forename: Irina B
authors.initials: IB
authors.lastname: Alchinova
authors.affiliation: Laboratory of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Baltiyskaya St., 8, Moscow, 125315, Russia.
authors.forename: Mikhail Yu
authors.initials: MY
authors.lastname: Karganov
authors.affiliation: Department of Chemistry and Methods of Chemistry Teaching, Orenburg State Pedagogical University, Sovetskaya st., 19, Orenburg, 460014, Russia.
authors.forename: Olga P
authors.initials: OP
authors.lastname: Ajsuvakova
authors.affiliation: Russian Society of Trace Elements in Medicine, ANO "Centre for Biotic Medicine", Zemlyanoy Val St. 46, Moscow 105064, Russia; Laboratory of Biotechnology and Applied Bioelementology, Yaroslavl State University, Sovetskaya st., 14, Yaroslavl, 150000, Russia; Institute of Bioelementology (Russian Satellite Centre of Trace Element - Institute for UNESCO), Orenburg State University, Pobedy Ave. 13, Orenburg 460352, Russia.
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Skalny
authors.affiliation: Department of Biochemistry, Orenburg State Medical University, Sovetskaya St., 6, Orenburg, 460000, Russia.
authors.forename: Alexandr A
authors.initials: AA
authors.lastname: Nikonorov
keywords: antagonism; lithium; metal distribution; toxic trace elements; zinc