title: Reverse Genetics of Respiratory Syncytial Virus.
abstract: Respiratory syncytial virus (RSV) is a negative-strand RNA virus that is associated with severe lower respiratory tract infections in young infants and the elderly. RSV remains a leading cause worldwide of infant mortality, and despite the high clinical and economic burden of the virus there are currently no available vaccines. Here, we describe the methods for recovery of recombinant RSV viruses using a bacterial artificial chromosome and methods related to procurement and expansion of stocks of RSV mutants.
authors.affiliation: Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA.|Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA.
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Stobart
authors.affiliation: Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA.|Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA.
authors.forename: Anne L
authors.initials: AL
authors.lastname: Hotard
authors.affiliation: Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA.|Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA.
authors.forename: Jia
authors.initials: J
authors.lastname: Meng
authors.affiliation: Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA. martin.moore@emory.edu.|Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA. martin.moore@emory.edu.
authors.forename: Martin L
authors.initials: ML
authors.lastname: Moore
mesh_terms: D002460:Cell Line; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007223:Infant; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D059386:Reverse Genetics / Q000379:methods*; D014169:Transformation, Bacterial
keywords: Bacterial artificial chromosome; RSV; Respiratory syncytial virus; Reverse genetics; Virus recovery