title: Hematopoietic effect of fermented deer antler extract in iron deficient diet-induced anemic rats.
abstract: OBJECTIVE
  To assess the hematopoietic effects of fermented deer antler extract using a dietinduced anemic animal model to facilitate the utilization of fermented deer antler extract and its derived products.
  
  
  METHODS
  Thirty 3-week-old female Sprague-Dawley rats were treated for 5 weeks. The rats were randomly divided into 6 groups and treated as follows: control, saline; NFA200, non-fermented deer antler extract 200 mg/kg; NFA500, non-fermented deer antler extract 500 mg/kg; FAB200, fermented deer antler extract 200 mg/kg; FAB500, fermented deer antler extract 500 mg/kg; and PC, heme iron 0.2 mg/kg. Blood parameters, iron content in the liver and spleen, hepatic δ-aminolevulinic acid dehydrogenase (ALAD) activity and divalent metal transporter 1 (DMT1) mRNA expression were analyzed.
  
  
  RESULTS
  No detectable significant differences were observed in blood parameters among groups. The decrease in the hepatic ALAD activity in anemic rats was significantly improved by fermented deer antler extract supplementation (P<0.05); however, non-fermented deer antler extract supplementation did not result in a significant improvement (P>0.05). The hepatic DMT1 mRNA expression level was increased significantly by supplementation with both the fermented deer antler extract and the non-fermented deer antler extract in a dose-dependent manner compared with nontreatment in anemic rats (P<0.05).
  
  
  CONCLUSIONS
  The hematopoietic activity induced by deer antler extract in dietinduced anemic rats might be increased through the fermentation process.
authors.affiliation: Department of Home Economic Education, Jeonju University, Jeonju, 55069, Republic of Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Jung
authors.affiliation: Institute of Biotechnology and Medical Converged Science, Dongguk University, Goyang, 10326, Republic of Korea.
authors.forename: Yooheon
authors.initials: Y
authors.lastname: Park
authors.affiliation: Kwang-Dong Pharmaceutical Co., Siheung, 14980, Republic of Korea.
authors.forename: Moon Jea
authors.initials: MJ
authors.lastname: Woo
authors.affiliation: Kwang-Dong Pharmaceutical Co., Siheung, 14980, Republic of Korea.
authors.forename: Sang Hun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Public Health Sciences, Graduate School, Korea University, Seoul, 02841, Republic of Korea. suh1960@korea.ac.kr.
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Suh
keywords: deer antler; divalent metal transporter 1; fermentation; iron deficiency anemia; δ-aminolevulinic acid dehydrogenase