title: Using Image-Based Flow Cytometry to Assess Monocyte Oxidized LDL Phagocytosis Capacity.
abstract: The examination of monocyte phagocytosis of modified lipoproteins is important to the understanding of plaque deposition and the development of atherosclerosis. Current methods lack the high-throughput image-based analysis capabilities, which may yield novel information concerning monocyte activity in disease processes. Specifically, this method identifies monocyte phagocytosis of oxidized LDL along with a change in adhesion molecules and scavenger receptors. Our laboratory is currently implementing this method as a means to study how acute dietary modifications alter risk of developing atherosclerosis.
authors.affiliation: Applied Physiology Laboratory, University of North Texas, Denton, TX, 76203, USA.|Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Andrea L
authors.initials: AL
authors.lastname: Henning
authors.affiliation: Applied Physiology Laboratory, University of North Texas, Denton, TX, 76203, USA.|Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Adam S
authors.initials: AS
authors.lastname: Venable
authors.affiliation: Applied Physiology Laboratory, University of North Texas, Denton, TX, 76203, USA.|Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Prado
authors.affiliation: Applied Physiology Laboratory, University of North Texas, Denton, TX, 76203, USA. brian.mcfarlin@unt.edu.|Department of Biological Sciences, University of North Texas, Denton, TX, USA. brian.mcfarlin@unt.edu.
authors.forename: Brian K
authors.initials: BK
authors.lastname: McFarlin
mesh_terms: D050197:Atherosclerosis / Q000473:pathology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism / Q000473:pathology*; D010587:Phagocytosis
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein
keywords: Adhesion molecules; Atherosclerosis; FlowSight; Foam cells; Imaging flow cytometry; Macrophage; Monocyte activity; Oxidized LDL; Phagocytosis