title: Hepatoprotective and antioxidant potential of Asphodeline lutea (L.) Rchb. roots extract in experimental models in vitro/in vivo.
abstract: The aim of this study was to investigate the effect of Asphodeline lutea (L.) Rchb. dry root extract (ALE) administered alone and against carbon tetrachloride (CCl4)-induced liver injury in vitro/in vivo. The dried roots of A. lutea were extracted with 70% ethanol and was characterized with HPLC-UV. Hepatoprotective potential was investigated by in vivo/in vitro assays in Wistar rats as well as antioxidant properties. At concentrations ranging from 10 to 200μg/mL of ALE significant cytotoxic effects on isolated hepatocytes were found. ALE showed some toxicity in Wistar rats discerned by increased ALT (Alanine transaminase), ALP (Alkaline phosphatase) activities and MDA (malondialdehyde) quantity, decreased GSH (reduced glutathione) levels without affecting the activity of the antioxidant enzymes (GPx (Gluthatione peroxidase), GR (Glutathione reductase) and GST (Glutathione-S-transferase activity)). The antioxidant and hepatoprotective potential of ALE was also observed in vitro/in vivo against CCl4-induced liver injury, where ALE normalizes all the examined parameters perturbated by CCl4administration. In addition, ALE preserved the decreased cytochrome P450 level and EMND (Ethylmorphine-N-Demethylase) activity without affecting AH (Aniline 4-Hydroxylase) activity. ALE is rich in anthraquinones, naphthalenes and caffeic acid. The pro-oxidant effects of ALE could be due to naphthalene and anthraquinone bioactivation pathways involving toxic metabolites.
authors.affiliation: Department of Chemistry, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Str., 1000 Sofia, Bulgaria.
authors.forename: Irina
authors.initials: I
authors.lastname: Lazarova
authors.affiliation: Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Str., 1000 Sofia, Bulgaria.
authors.forename: Rumyana
authors.initials: R
authors.lastname: Simeonova
authors.affiliation: Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Str., 1000 Sofia, Bulgaria.
authors.forename: Vessela
authors.initials: V
authors.lastname: Vitcheva
authors.affiliation: Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Str., 1000 Sofia, Bulgaria.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kondeva-Burdina
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Str., 1000 Sofia, Bulgaria.
authors.forename: Reneta
authors.initials: R
authors.lastname: Gevrenova
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Str., 1000 Sofia, Bulgaria.
authors.forename: Dimitrina
authors.initials: D
authors.lastname: Zheleva-Dimitrova
authors.affiliation: Department of Biology, Faculty of Science, Selcuk University, Afyon-Konya Road, 42250 Konya, Turkey. Electronic address: gokhanzengin@selcuk.edu.tr.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Zengin
authors.affiliation: Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Str., 1000 Sofia, Bulgaria.
authors.forename: Nikolay D
authors.initials: ND
authors.lastname: Danchev
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D000070357:Asparagaceae / Q000737:chemistry*; D002251:Carbon Tetrachloride; D002469:Cell Separation; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D008297:Male; D064209:Phytochemicals / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D020011:Protective Agents / Q000494:pharmacology*; D017208:Rats, Wistar; D012015:Reference Standards; D012838:Silymarin / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts; D020011:Protective Agents; D012838:Silymarin; D002251:Carbon Tetrachloride
keywords: Anthraquinones; Asphodeline lutea; CCl(4); Cytochrome P450; Hepatocytes; Oxidative stress