title: Differentiation of Trypanosoma cruzi I (TcI) and T. cruzi II (TcII) genotypes using genes encoding serine carboxypeptidases.
abstract: The parasite Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) can be classified based on biochemical and molecular markers, into six lineages or discrete typing units (DTUs), T. cruzi I-VI (TcI-VI), from which TcI and TcII are the parental genotypes. Trying to understand the dispersion of the subpopulations of T. cruzi in nature and its complex transmission cycles, the serine carboxypeptidase genes of T. cruzi were used as a molecular marker in the present study. DTUs of 25 T. cruzi isolates derived from different hosts and from different regions of Brazil were classified. Using specific primers, the complete serine carboxypeptidase open reading frame of 1401 bp was sequenced. The obtained data shows significant differences in the sequences of TcI and TcII. The analysis of the T. cruzi significantly different serine carboxypeptidase genes allowed distinguishing between the parental DTUs TcI to TcII and the hybrid DTU TcVI which grouped within the latter branch. The sequence diversity within the T. cruzi subpopulations was rather low. The analysis using the genes encoding proteases seems to be an interesting approach for the reconstruction of the origin and genotype evolution of T. cruzi.
authors.affiliation: Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz (IOC), Av. Brasil 4365 Manguinhos, CEP: 21045-900, Rio de Janeiro, Brazil. cacatarinarj@gmail.com.
authors.forename: Catarina Andréa Chaves
authors.initials: CA
authors.lastname: de Araújo
authors.affiliation: Museum Alexander Koenig, Adenauerallee 160, 53113, Bonn, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Mayer
authors.affiliation: Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Av. Brasil 4365 Manguinhos, CEP: 21045-900, Rio de Janeiro, Brazil.
authors.forename: Peter Josef
authors.initials: PJ
authors.lastname: Waniek
authors.affiliation: Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Av. Brasil 4365 Manguinhos, CEP: 21045-900, Rio de Janeiro, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Azambuja
authors.affiliation: Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz (IOC), Av. Brasil 4365 Manguinhos, CEP: 21045-900, Rio de Janeiro, Brazil.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Jansen
mesh_terms: D005075:Biological Evolution; D001938:Brazil; D002268:Carboxypeptidases / Q000235:genetics*; D014355:Chagas Disease / Q000469:parasitology*; D005838:Genotype; D006801:Humans; D015800:Protozoan Proteins / Q000235:genetics*; D014349:Trypanosoma cruzi / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D015800:Protozoan Proteins; D002268:Carboxypeptidases; C022633:serine carboxypeptidase
keywords: Chagas disease; Genotypes; Serine carboxypeptidases; Transmission cycles; Trypanosoma cruzi I and II