title: Expression of a Grapevine NAC Transcription Factor Gene Is Induced in Response to Powdery Mildew Colonization in Salicylic Acid-Independent Manner.
abstract: Tissue colonization by grape powdery mildew (PM) pathogen Erysiphe necator (Schw.) Burr triggers a major remodeling of the transcriptome in the susceptible grapevine Vitis vinifera L. While changes in the expression of many genes bear the signature of salicylic acid (SA) mediated regulation, the breadth of PM-induced changes suggests the involvement of additional regulatory networks. To explore PM-associated gene regulation mediated by other SA-independent systems, we designed a microarray experiment to distinguish between transcriptome changes induced by E. necator colonization and those triggered by elevated SA levels. We found that the majority of genes responded to both SA and PM, but certain genes were responsive to PM infection alone. Among them, we identified genes of stilbene synthases, PR-10 proteins, and several transcription factors. The microarray results demonstrated that the regulation of these genes is either independent of SA, or dependent, but SA alone is insufficient to bring about their regulation. We inserted the promoter-reporter fusion of a PM-responsive transcription factor gene into a wild-type and two SA-signaling deficient Arabidopsis lines and challenged the resulting transgenic plants with an Arabidopsis-adapted PM pathogen. Our results provide experimental evidence that this grape gene promoter is activated by the pathogen in a SA-independent manner.
authors.affiliation: Institute of Genetics and Biotechnology, Szent Istvan University, 2100-Godollo, Hungary.
authors.forename: Zsofia
authors.initials: Z
authors.lastname: Toth
authors.affiliation: Institute of Crop Science, University of Hohenheim, 70599-Stuttgart, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Winterhagen
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, 2462-Martonvasar, Hungary.
authors.forename: Balazs
authors.initials: B
authors.lastname: Kalapos
authors.affiliation: Department of Mathematics, Missouri State University, 65897-Springfield, USA.
authors.forename: Yingcai
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Biology, Missouri State University, 65897-Springfield, USA.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Kovacs
authors.affiliation: Institute of Genetics and Biotechnology, Szent Istvan University, 2100-Godollo, Hungary.
authors.forename: Erzsebet
authors.initials: E
authors.lastname: Kiss
mesh_terms: D000217:Acyltransferases / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics*; D002473:Cell Wall / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000187:drug effects / Q000235:genetics; D054884:Host-Pathogen Interactions / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D004718:Saccharomycetales / Q000254:growth & development*; D020156:Salicylic Acid / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics; D027843:Vitis / Q000235:genetics* / Q000382:microbiology*
chemical_list: D010940:Plant Proteins; D000217:Acyltransferases; C022674:stilbene synthase; D020156:Salicylic Acid