title: Combination of soya pulp and Bacillus coagulans lilac-01 improves intestinal bile acid metabolism without impairing the effects of prebiotics in rats fed a cholic acid-supplemented diet.
abstract: Intestinal bacteria are involved in bile acid (BA) deconjugation and/or dehydroxylation and are responsible for the production of secondary BA. However, an increase in the production of secondary BA modulates the intestinal microbiota due to the bactericidal effects and promotes cancer risk in the liver and colon. The ingestion of Bacillus coagulans improves constipation via the activation of bowel movement to promote defaecation in humans, which may alter BA metabolism in the intestinal contents. BA secretion is promoted with high-fat diet consumption, and the ratio of cholic acid (CA):chenodeoxycholic acid in primary BA increases with ageing. The dietary supplementation of CA mimics the BA environment in diet-induced obesity and ageing. We investigated whether B. coagulans lilac-01 and soya pulp influence both BA metabolism and the maintenance of host health in CA-supplemented diet-fed rats. In CA-fed rats, soya pulp significantly increased the production of secondary BA such as deoxycholic acid and ω-muricholic acids, and soya pulp ingestion alleviated problems related to plasma adiponectin and gut permeability in rats fed the CA diet. The combination of B. coagulans and soya pulp successfully suppressed the increased production of secondary BA in CA-fed rats compared with soya pulp itself, without impairing the beneficial effects of soya pulp ingestion. In conclusion, it is possible that a combination of prebiotics and probiotics can be used to avoid an unnecessary increase in the production of secondary BA in the large intestine without impairing the beneficial functions of prebiotics.
authors.affiliation: 1Research Faculty of Agriculture,Hokkaido University,Sapporo 060-8589,Japan.
authors.forename: Yeonmi
authors.initials: Y
authors.lastname: Lee
authors.affiliation: 1Research Faculty of Agriculture,Hokkaido University,Sapporo 060-8589,Japan.
authors.forename: Reika
authors.initials: R
authors.lastname: Yoshitsugu
authors.affiliation: 1Research Faculty of Agriculture,Hokkaido University,Sapporo 060-8589,Japan.
authors.forename: Keidai
authors.initials: K
authors.lastname: Kikuchi
authors.affiliation: 1Research Faculty of Agriculture,Hokkaido University,Sapporo 060-8589,Japan.
authors.forename: Ga-Hyun
authors.initials: GH
authors.lastname: Joe
authors.affiliation: 1Research Faculty of Agriculture,Hokkaido University,Sapporo 060-8589,Japan.
authors.forename: Misaki
authors.initials: M
authors.lastname: Tsuji
authors.affiliation: 1Research Faculty of Agriculture,Hokkaido University,Sapporo 060-8589,Japan.
authors.forename: Takuma
authors.initials: T
authors.lastname: Nose
authors.affiliation: 1Research Faculty of Agriculture,Hokkaido University,Sapporo 060-8589,Japan.
authors.forename: Hidehisa
authors.initials: H
authors.lastname: Shimizu
authors.affiliation: 1Research Faculty of Agriculture,Hokkaido University,Sapporo 060-8589,Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hara
authors.affiliation: 3Arterio Bio Co., Ltd,Otaru 047-0261,Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Minamida
authors.affiliation: 3Arterio Bio Co., Ltd,Otaru 047-0261,Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Miwa
authors.affiliation: 1Research Faculty of Agriculture,Hokkaido University,Sapporo 060-8589,Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ishizuka
mesh_terms: D000818:Animals; D000070000:Bacillus coagulans; D001647:Bile Acids and Salts / Q000378:metabolism*; D019826:Cholic Acid / Q000008:administration & dosage*; D019587:Dietary Supplements; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology; D010936:Plant Extracts / Q000378:metabolism*; D056692:Prebiotics; D051381:Rats; D013025:Glycine max; D058616:Synbiotics
chemical_list: D001647:Bile Acids and Salts; D010936:Plant Extracts; D056692:Prebiotics; D019826:Cholic Acid
keywords: ALT alanine aminotransaminase; AST aspartate aminotransferase; BA bile acid; Bacillus coagulans lilac-01; Bile acids; CA cholic acid; CDCA chenodeoxycholic acid; DCA deoxycholic acid; Gut permeability; LCA lithocholic acid; Soya pulp; Syn synbiotic diet; Synbiotics