title: miR-1299 suppresses cell proliferation of hepatocellular carcinoma (HCC) by targeting CDK6.
abstract: microRNA (miRNA) plays critical role in HCC initiation and development, many miRNAs have been reported to regulate HCC progression. In this study, we studied the role of miR-1299 in cell proliferation of HCC. We found miR-1299 was significantly downregulated in HCC cells and tissues. miR-1299 overexpression inhibited cell proliferation and arrested cell cycle in G0/G1 phase analyzed by MTT assay, soft agar assay, BrdU cell proliferation assay and cell cycle assay, while miR-1299 knockdown promoted cell proliferation and accelerated G1/S transition. Further analysis suggested the key regulator of G1/S transition, cyclin-dependent kinase 6 (CDK6) was the target of miR-1299, miR-1299 inhibited CDK6 expression and bound to the 3'UTR of CDK6. When double knockdown of miR-1299 and CDK6 promoted cell proliferation copied the phenotype caused by miR-1299 overexpression, suggesting miR-1299 inhibits cell proliferation by targeting CDK6. In summary, our data revealed miR-1299 inhibits cell proliferation, and might be a target for HCC therapy.
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China.
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, ‍Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China.
authors.forename: Guangchuan
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China.
authors.forename: Xie
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China.
authors.forename: Hengjun
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chang
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China.
authors.forename: Hongguang
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China.
authors.forename: Fang-Feng
authors.initials: FF
authors.lastname: Liu
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Anesthesiology, Shandong Provincial Hospital Affiliated to Shandong University, No. 9677 Jingshi Road Jinan 250014, Shandong Province, China. Electronic address: majinben@sina.com.
authors.forename: Jinben
authors.initials: J
authors.lastname: Ma
mesh_terms: D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051361:Cyclin-Dependent Kinase 6 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
chemical_list: C000610113:MIRN1299 microRNA, human; D035683:MicroRNAs; C495911:CDK6 protein, human; D051361:Cyclin-Dependent Kinase 6
keywords: CDK6; Cell proliferation; HCC; miR-1299