title: Interactions of α-Factor-1, a Yeast Pheromone, and Its Analogue with Copper(II) Ions and Low-Molecular-Weight Ligands Yield Very Stable Complexes.
abstract: α-Factor-1 (WHWLQLKPGQPMY), a peptidic pheromone of Saccharomyces cerevisiae yeast, contains a XHX type copper(II) binding N-terminal site. Using a soluble analogue, WHWSKNR-amide, we demonstrated that the W(1)H(2)W(3) site alone binds copper(II) with a Kd value of 0.18 pM at pH 7.4 and also binds imidazole (Im) in a ternary complex (Kd of 1 mM at pH 7.4). This interaction boosts the ability of the peptide to sequester copper(II) depending on the Im concentration up to a subfemtomolar range, not available for any oligopeptidic system studied before. Therefore, α-factor-1 and other XHX-type peptides are likely copper(II) carriers in biological systems.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences , Pawińskiego 5a, 02-106 Warsaw, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Bossak
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences , Pawińskiego 5a, 02-106 Warsaw, Poland.|Florey Department of Neuroscience and Mental Health, The University of Melbourne , Melbourne, Victoria 3010, Australia.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Mital
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences , Pawińskiego 5a, 02-106 Warsaw, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Poznański
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences , Pawińskiego 5a, 02-106 Warsaw, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Bonna
authors.affiliation: Florey Department of Neuroscience and Mental Health, The University of Melbourne , Melbourne, Victoria 3010, Australia.
authors.forename: Simon
authors.initials: S
authors.lastname: Drew
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences , Pawińskiego 5a, 02-106 Warsaw, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Bal
mesh_terms: D000577:Amides / Q000737:chemistry; D001665:Binding Sites; D002942:Circular Dichroism; D003300:Copper / Q000378:metabolism*; D007093:Imidazoles / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D000072235:Mating Factor / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000577:Amides; D007093:Imidazoles; D008024:Ligands; C000606786:alpha-factor (Saccharomyces cerevisiae); D000072235:Mating Factor; D003300:Copper; C029899:imidazole