title: Synthesis of Leucas Aspera Extract Loaded Gold-PLA-PEG-PLA Amphiphilic Copolymer Nanoconjugates: In Vitro Cytotoxicity and Anti-Inflammatory Activity Studies.
abstract: Gold nanoparticles (GNPs) are synthesized using the medicinal plant Leucas Aspera extract (LAE) and poly lactic acid-co-poly ethylene glycol-co-poly lactic acid (PLA-PEG-PLA) copolymer by water-in-oil (W/O) emulsion method. The proposed method of W/O emulsion technique involves synthesis of GNPs and loading of Leucas Aspera extract on to the PLA-PEG-PLA copolymer matrix simultaneously. The synthesized GNPs are characterized by Fourier transform infra-red (FTIR) spectroscopy, dynamic light scattering (DLS), X-ray diffractometry (XRD) and transmission electron microscopy (TEM). The GNPs-LAE loaded polymer NPs are examined for the in vitro cytotoxicity on South African green monkey's kidney cells. The GNPs-LAE loaded polymer nanoconjugates exhibit maximum up to 95% of cell viability with 100 μg concentration of GNPs in the sample. The GNPs-LAE loaded polymer NPs exhibit better anti-inflammatory activity when compared to the pure LAE.
authors.forename: K
authors.initials: K
authors.lastname: Reena
authors.forename: P
authors.initials: P
authors.lastname: Balashanmugam
authors.forename: M
authors.initials: M
authors.lastname: Gajendiran
authors.forename: S Arul
authors.initials: SA
authors.lastname: Antony
mesh_terms: D065966:Absorption, Physicochemical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000138:chemical synthesis; D001769:Blood / Q000187:drug effects / Q000276:immunology*; D002522:Chlorocebus aethiops; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000633:toxicity; D006046:Gold / Q000008:administration & dosage / Q000633:toxicity*; D007773:Lactates / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000633:toxicity*; D053769:Nanocapsules; D058726:Nanoconjugates / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D014709:Vero Cells
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D007773:Lactates; D053769:Nanocapsules; D058726:Nanoconjugates; D013501:Surface-Active Agents; C088513:poly(lactic acid-ethylene glycol); D011092:Polyethylene Glycols; D006046:Gold