title: Cellular tagging as a neural network mechanism for behavioural tagging.
abstract: Behavioural tagging is the transformation of a short-term memory, induced by a weak experience, into a long-term memory (LTM) due to the temporal association with a novel experience. The mechanism by which neuronal ensembles, each carrying a memory engram of one of the experiences, interact to achieve behavioural tagging is unknown. Here we show that retrieval of a LTM formed by behavioural tagging of a weak experience depends on the degree of overlap with the neuronal ensemble corresponding to a novel experience. The numbers of neurons activated by weak training in a novel object recognition (NOR) task and by a novel context exploration (NCE) task, denoted as overlapping neurons, increases in the hippocampal CA1 when behavioural tagging is successfully achieved. Optical silencing of an NCE-related ensemble suppresses NOR-LTM retrieval. Thus, a population of cells recruited by NOR is tagged and then preferentially incorporated into the memory trace for NCE to achieve behavioural tagging.
authors.affiliation: Department of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.|CREST, JST, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Nomoto
authors.affiliation: Department of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.|CREST, JST, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Ohkawa
authors.affiliation: CREST, JST, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.|Division of Animal Experimental Laboratory, Life Science Research Center, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nishizono
authors.affiliation: Department of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.|CREST, JST, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Yokose
authors.affiliation: Department of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.|CREST, JST, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Akinobu
authors.initials: A
authors.lastname: Suzuki
authors.affiliation: Division of Animal Experimental Laboratory, Life Science Research Center, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Mina
authors.initials: M
authors.lastname: Matsuo
authors.affiliation: Department of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.|CREST, JST, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Tsujimura
authors.affiliation: Department of Neuroscience, Jikei University School of Medicine, Tokyo 105-8461, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Department of Neuroscience, Jikei University School of Medicine, Tokyo 105-8461, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Nagase
authors.affiliation: Department of Neuroscience, Jikei University School of Medicine, Tokyo 105-8461, Japan.
authors.forename: Ayako M
authors.initials: AM
authors.lastname: Watabe
authors.affiliation: Department of Neuroscience, Jikei University School of Medicine, Tokyo 105-8461, Japan.
authors.forename: Fusao
authors.initials: F
authors.lastname: Kato
authors.affiliation: Department of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.|CREST, JST, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Inokuchi
mesh_terms: D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D001522:Behavior, Animal / Q000502:physiology*; D056547:CA1 Region, Hippocampal / Q000166:cytology / Q000502:physiology; D008297:Male; D057567:Memory, Long-Term / Q000502:physiology*; D008570:Memory, Short-Term / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D023421:Models, Animal; D009415:Nerve Net / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D021641:Recognition, Psychology / Q000187:drug effects / Q000502:physiology
chemical_list: D011500:Protein Synthesis Inhibitors; D000841:Anisomycin