title: Effects of a Soluble Epoxide Hydrolase Inhibitor on Lipopolysaccharide-Induced Acute Lung Injury in Mice.
abstract: OBJECTIVE
  Inflammation plays a key role in the pathogenesis of acute lung injury (ALI). Soluble epoxide hydrolase (sEH) is suggested as a vital pharmacologic target for inflammation. In this study, we determined whether a sEH inhibitor, AUDA, exerts lung protection in lipopolysaccharide (LPS)-induced ALI in mice.
  
  
  METHODS
  Male BALB/c mice were randomized to receive AUDA or vehicle intraperitoneal injection 4 h after LPS or phosphate buffered saline (PBS) intratracheal instillation. Samples were harvested 24 h post LPS or PBS administration.
  
  
  RESULTS
  AUDA administration decreased the pulmonary levels of monocyte chemoattractant protein (MCP)-1 and tumor necrosis factor (TNF)-α. Improvement of oxygenation and lung edema were observed in AUDA treated group. AUDA significantly inhibited sEH activity, and elevated epoxyeicosatrienoic acids (EETs) levels in lung tissues. Moreover, LPS induced the activation of nuclear factor (NF)-κB was markedly dampened in AUDA treated group.
  
  
  CONCLUSIONS
  Administration of AUDA after the onset of LPS-induced ALI increased pulmonary levels of EETs, and ameliorated lung injury. sEH is a potential pharmacologic target for ALI.
authors.affiliation: Department of Burn and Plastic Surgery, Subei People's Hospital of Jiangsu province, Jiangsu, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tao
authors.affiliation: Department of Burn and Plastic Surgery, Subei People's Hospital of Jiangsu province, Jiangsu, People's Republic of China.
authors.forename: Ping-Song
authors.initials: PS
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Subei People's Hospital of Jiangsu province, Jiangsu, People's Republic of China.
authors.forename: Liu-Qing
authors.initials: LQ
authors.lastname: Yang
authors.affiliation: Department of Outpatient, Subei People's Hospital of Jiangsu province, Jiangsu, People's Republic of China.
authors.forename: Yong-Bo
authors.initials: YB
authors.lastname: Ma
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy / Q000201:enzymology; D000818:Animals; D018932:Chemokine CCL2 / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004851:Epoxide Hydrolases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D004851:Epoxide Hydrolases