title: Reversal of Lead-Induced Acute Toxicity by Lipoic Acid with Nutritional Supplements in Male Wistar Rats.
abstract: Lead (Pb) is a pleiotropic toxicant. The potential role of oxidative stress injury that is associated with Pb poisoning suggests that antioxidants may enhance the efficacy of treatment designed to mitigate Pb-induced toxicity. The aim of this study is to investigate the comparative ameliorative potential of lipoic acid (LA) alone or in combination with calcium (Ca) and zinc (Zn). Pb acetate (50 mg/kg, intraperitoneally) was administered for 3 d. After 24 h of the last toxicant dose, LA (100 mg/kg, orally [po]) alone or in conjuction with Ca (50 mg/kg, po) and Zn (10 mg/kg, po) was administered for 3 d. Significant alterations in the concentration of urea, uric acid, triglycerides, cholesterol, aspartate amino transferase, alanine amino transferase, lipid peroxidation, and reduced glutathione as well as alterations in enzyme activity of δ-aminolevulinic acid (ALA) dehydratase were observed following acute Pb exposure. These findings were also supported by elevated mean DNA damage and Pb body burden in blood and soft tissues compared to controls (p ≤ 0.05). Three d posttreatment with LA along with Zn and Ca could significantly restore the biochemical parameters and Pb body burden to near-normal status through antioxidant activity or by preventing bioaccumulation of Pb within the blood and tissues of experimental rats.
authors.affiliation: School of Studies in Zoology, Jiwaji University, Gwalior 474011, India.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Shukla
authors.affiliation: School of Studies in Zoology, Jiwaji University, Gwalior 474011, India.
authors.forename: Yamini
authors.initials: Y
authors.lastname: Sharma
authors.affiliation: Reproductive Biology and Toxicology Labora¬tory, UNESCO Satellite Center of Trace Element Research, School of Studies in Zoology, Jiwaji University, Gwalior 474011, India.
authors.forename: Sadhana
authors.initials: S
authors.lastname: Shrivastava
mesh_terms: D000818:Animals; D002119:Calcium Carbonate / Q000008:administration & dosage*; D004249:DNA Damage; D004032:Diet; D019587:Dietary Supplements / Q000032:analysis; D008297:Male; D009942:Organometallic Compounds / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D008063:Thioctic Acid / Q000008:administration & dosage*; D019287:Zinc Sulfate / Q000008:administration & dosage*
chemical_list: D009942:Organometallic Compounds; D008063:Thioctic Acid; D019287:Zinc Sulfate; D002119:Calcium Carbonate; C008261:lead acetate