title: Development and validation of a quantification method for oleanolic acid and hederagenin in rat plasma: application to the pharmacokinetic study.
abstract: A rapid and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed and validated for simultaneous quantification of oleanolic acid and hederagenin in rat plasma. After the two analytes were extracted with liquid-liquid extraction, chromatographic separation was performed on a C18column with acetonitrile and water (85:15, v/v) as mobile phase at a flow rate of 0.4 mL/min. Calibration curves exhibited good linearity (r > 0.995) over the ranges of 0.41-82.0 ng/mL for oleanolic acid and 0.32-64.0 ng/mL for hederagenin, respectively. The lower limit of quantifications (LLOQs) in plasma were 0.41 ng/mL for oleanolic acid and 0.32 ng/mL for hederagenin. The established LLOQs were within the concentration needed for the assay in plasma, which met the requirements to evaluate their pharmacokinetics of oleanolic acid and hederagenin. This developed assay was successfully applied in the pharmacokinetic study of oleanolic acid and hederagenin in rats after oral administration of Rhizoma Clematidis extract.
authors.affiliation: Department of Pharmacy, Hiser Medical Center of Qingdao, Qingdao, People's Republic of China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, the Affiliated Hospital of Qingdao University, People's Republic of China.
authors.forename: Fanbo
authors.initials: F
authors.lastname: Jing
authors.affiliation: Department of Pharmacy, Linyi People's Hospital, Linyi, People's Republic of China.
authors.forename: Zonglin
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D039324:Clematis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000493:pharmacokinetics; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction / Q000379:methods; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D009828:Oleanolic Acid; C025763:hederagenin
keywords: Rhizoma Clematidis; hederagenin; oleanolic acid; pharmacokinetic studies; rat plasma