title: Elevated E2F7 expression predicts poor prognosis in human patients with gliomas.
abstract: E2F transcription factors have been studied extensively in a broad range of organisms as major regulators of cell cycle, apoptosis, and differentiation. The E2F family includes the atypical member E2F7, which has been rarely studied in gliomas. The aim of this study is to determine the expression status of E2F7 in gliomas, its relationship to clinicopathological features, and patients' outcome. The mRNA levels of E2F7 in the human brain and different grades of gliomas were analysed using datasets from the publically available Oncomine database. One of the most significant co-expression factors, CDK1, together with E2F7, was further validated by immunohistochemistry in 90 different grades of gliomas. Furthermore, univariate and multivariate analyses were performed to identify prognostic variables relative to patient and tumour characteristics and treatment modalities. E2F7 mRNA expression was found to be elevated in gliomas by Oncomine-database analysis. Immunohistochemistry showed an increase in E2F7 labelling index in high- versus low-grade gliomas (62.1±11.8% vs. 18.9±10.2%, p<0.0001). There was a positive correlation between E2F7 and CDK1 immunoreactivity (Spearman r=0.446, p=0.037). Clinicopathological evaluation suggested that E2F7 expression was associated with tumour grade (p<0.0001) and recurrence (p=0.025). In Cox multivariate analysis, pathological classification and recurrence were independent prognostic factors of gliomas, and E2F7 was significantly related to progression-free survival (p=0.011), but not overall survival (p=0.062). Our findings suggested that E2F7 might act as an independent prognostic factor of gliomas and might constitute a potential therapeutic target for this disease.
authors.affiliation: The First People's Hospital of Xuzhou, No.19, North Zhongshan Road, Quanshan District, Xuzhou, Jiangsu Province 221002, China.
authors.forename: WenWen
authors.initials: W
authors.lastname: Yin
authors.affiliation: The First People's Hospital of Xuzhou, No.19, North Zhongshan Road, Quanshan District, Xuzhou, Jiangsu Province 221002, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, XuZhou Central Hospital, Xuzhou, China.
authors.forename: MaoHua
authors.initials: M
authors.lastname: Ding
authors.affiliation: The First People's Hospital of Xuzhou, No.19, North Zhongshan Road, Quanshan District, Xuzhou, Jiangsu Province 221002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huo
authors.affiliation: The First People's Hospital of Xuzhou, No.19, North Zhongshan Road, Quanshan District, Xuzhou, Jiangsu Province 221002, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Hu
authors.affiliation: The First People's Hospital of Xuzhou, No.19, North Zhongshan Road, Quanshan District, Xuzhou, Jiangsu Province 221002, China.
authors.forename: RuoNan
authors.initials: R
authors.lastname: Cai
authors.affiliation: The First People's Hospital of Xuzhou, No.19, North Zhongshan Road, Quanshan District, Xuzhou, Jiangsu Province 221002, China.
authors.forename: TingTing
authors.initials: T
authors.lastname: Zhou
authors.affiliation: The First People's Hospital of Xuzhou, No.19, North Zhongshan Road, Quanshan District, Xuzhou, Jiangsu Province 221002, China.
authors.forename: ZaoHua
authors.initials: Z
authors.lastname: Gao
authors.affiliation: The First People's Hospital of Xuzhou, No.19, North Zhongshan Road, Quanshan District, Xuzhou, Jiangsu Province 221002, China. Electronic address: dyyywzl@126.com.
authors.forename: ZaoLing
authors.initials: Z
authors.lastname: Wang
authors.affiliation: The First People's Hospital of Xuzhou, No.19, North Zhongshan Road, Quanshan District, Xuzhou, Jiangsu Province 221002, China. Electronic address: chendezhixz@163.com.
authors.forename: DeZhi
authors.initials: D
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D016203:CDC2 Protein Kinase / Q000096:biosynthesis / Q000235:genetics; D016208:Databases, Factual; D018572:Disease-Free Survival; D050702:E2F7 Transcription Factor / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005910:Glioma / Q000175:diagnosis* / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009364:Neoplasm Recurrence, Local; D011237:Predictive Value of Tests; D011379:Prognosis; D016019:Survival Analysis
chemical_list: D050702:E2F7 Transcription Factor; D016203:CDC2 Protein Kinase
keywords: CDK1; E2F7; Glioma; Prognosis; Tumor grade