title: Surface Roughness Modulates Diffusion and Fibrillation of Amyloid-β Peptide.
abstract: The presence of surfaces influences the kinetics of amyloid-β (Aβ) peptide fibrillation. Although it has been generally recognized that the fibrillation process can be assisted or accelerated by surface chemistry, the impact of surface topography, i.e., roughness, on peptide fibrillation is relatively little understood. Here we study the role of surface roughness on surface-mediated fibrillation using polymer coatings of varying roughness as well as polymer microparticles. Using single-molecule tracking, atomic force microscopy, and the thioflavin T fluorescence technique, we show that a rough surface decelerates the two-dimensional (2D) diffusion of peptides and retards the surface-mediated fibrillation. A higher degree of roughness that presents an obstacle to peptide diffusion is found to inhibit the fibrillation process.
authors.affiliation: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
authors.forename: Khurram
authors.initials: K
authors.lastname: Shezad
authors.affiliation: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
authors.forename: Kejun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
authors.forename: Mubashir
authors.initials: M
authors.lastname: Hussain
authors.affiliation: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Dong
authors.affiliation: College of Chemistry and Environmental Engineering, Shenzhen University , Shenzhen 518060, China.
authors.forename: Chuanxin
authors.initials: C
authors.lastname: He
authors.affiliation: School of Materials Science and Engineering, South China University of Technology , Guangzhou 510640, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Gong
authors.affiliation: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Xie
authors.affiliation: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
authors.forename: Jintao
authors.initials: J
authors.lastname: Zhu
authors.affiliation: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shen
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D052160:Benzothiazoles; D006801:Humans; D018625:Microscopy, Atomic Force; D013499:Surface Properties; D013844:Thiazoles / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D013844:Thiazoles; C009462:thioflavin T