title: Evaluation of micro Electroretinograms Recorded with Multiple Electrode Array to Assess Focal Retinal Function.
abstract: Full-field electroretinograms (ERGs) are used to objectively assess the mass function of the retina, whereas focal ERGs are used to evaluate the focal retinal function. The purpose of this study was to determine the usefulness of a multiple electrode array (MEA) system for recording ex vivo micro ERGs (mERGs) together with multiunit spike responses of the retinal ganglion cells (RGCs) to assess focal retinal function in isolated mouse retinas. The a- and b-waves of the full-field ERGs were present in the mERG. The b-wave was blocked by L-AP4, an inhibitor of the mGluR6 receptor, and the OFF-component was blocked by exposure to PDA, an antagonist of ionotropic glutamate receptors, with a corresponding RGC responses. mERGs were also recorded from mice with progressive retinal degeneration, the C57BL/6J-Pde6b(rd1-2J)/J (rd1) mice, from which conventional full-field ERGs are non-recordable. A blockade of the glutamate receptors indicated that the negative wave of rd1 mice do not originate from the photoreceptors but from the second or third order neurons. This technique of recording mERGs will be useful in assessing the focal properties of the retinas obtained from eyes with pathology and also to follow the recovery of the physiology of the retina in regenerative studies.
authors.affiliation: Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, 650-0047, Japan.
authors.forename: Momo
authors.initials: M
authors.lastname: Fujii
authors.affiliation: Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, 650-0047, Japan.
authors.forename: Genshiro A
authors.initials: GA
authors.lastname: Sunagawa
authors.affiliation: Department of Ophthalmology, Mie University Graduate School of Medicine, 2-174, Edobashi, Tsu, Mie, 514-8507, Japan.
authors.forename: Mineo
authors.initials: M
authors.lastname: Kondo
authors.affiliation: Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, 650-0047, Japan.
authors.forename: Masayo
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, 650-0047, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Mandai
mesh_terms: D000818:Animals; D003839:Deoxyadenosines / Q000494:pharmacology; D004195:Disease Models, Animal; D004566:Electrodes; D004596:Electroretinography / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology*; D011422:Propionates / Q000494:pharmacology; D012160:Retina / Q000503:physiopathology*; D012162:Retinal Degeneration / Q000503:physiopathology*; D012165:Retinal Ganglion Cells / Q000502:physiology
chemical_list: C114785:2-amino-4-phosphono-propinate; C101165:7-(1-propynyl)-7-deaza-2'-deoxyadenosine; D003839:Deoxyadenosines; D011422:Propionates