title: Immunohistochemistry and Polymerase Chain Reaction for Detection Human Papilloma Virus in Warts: A Comparative Study.
abstract: BACKGROUND
  Immunohistochemistry and polymerase chain reaction (PCR) are the most widely used methods for the detection of viruses. PCR is known to be a more sensitive and specific method than the immunohistochemical method at this time, but PCR has the disadvantages of high cost and skilled work to use widely. With the progress of technology, the immunohistochemical methods used in these days has come to be highly sensitive and actively used in the diagnostic fields.
  
  
  OBJECTIVE
  To evaluate and compare the usefulness of immunohistochemistry and PCR for detection human papilloma virus (HPV) in wart lesions.
  
  
  METHODS
  Nine biopsy samples of verruca vulgaris and 10 of condyloma accuminatum were examined. Immunohistochemical staining using monoclonal antibody to HPV L1 capsid protein and PCR were done for the samples. DNA sequencing of the PCR products and HPV genotyping were also done.
  
  
  RESULTS
  HPV detection rate was 78.9% (88.9% in verruca vulgaris, 70.0% in condyloma accuminatum) on immunohistochemistry and 100.0% for PCR. HPV-6 genotype showed a lower positivity rate on immunohistochemistry (50.0%) as compared to that of the other HPV genotypes.
  
  
  CONCLUSIONS
  Immunohistochemistry for HPV L1 capsid protein showed comparable sensitivity for detection HPV. Considering the high cost and great effort needed for the PCR methods, we can use immunohistochemistry for HPV L1 capsid protein with the advantage of lower cost and simple methods for HPV detection.
authors.affiliation: Department of Dermatology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Hong Sun
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Department of Dermatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Dermatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Ji Yoon
authors.initials: JY
authors.lastname: Choo
authors.affiliation: Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea.; Institute of Dermatological Science, Medical Research Center, Seoul National University, Seoul, Korea.; Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea.
authors.forename: Hee Jin
authors.initials: HJ
authors.lastname: Byun
authors.affiliation: Department of Bio-Medical Laboratory Science, Eulji Medi-Bio Research Institute, Eulji University, Seongnam, Korea.
authors.forename: Jin Hyun
authors.initials: JH
authors.lastname: Jun
authors.affiliation: Department of Dermatology, College of Medicine, The Catholic University of Korea, Seoul, Korea.; Department of Dermatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Jun Young
authors.initials: JY
authors.lastname: Lee
keywords: HPV L1 protein; Immunohistochemistry; Papillomaviridae; Polymerase chain reaction; human papillomavirus