title: Improved antioxidant and anti-tyrosinase activity of polysaccharide from Sargassum fusiforme by degradation.
abstract: An efficient method for the degradation of polysaccharides isolated from Sargassum fusiforme (PSF) was developed by using ascorbic acid in combination with H2O2. The degradation conditions were optimized using a Box-Behnken response surface design (BBRS). The optimum conditions were established as: concentration of ascorbic acid (VC) and H2O217.26mM, degradation temperature 51°C and degradation time 1.6h. The DPPH radical scavenging rate of the degraded polysaccharides from S. fusiforme (DPSF) obtained under the optimal conditions was determined to be 75.22±0.02%, which was well matched with the value (75.21%) predicted by the BBRS model. In vitro antioxidant activity of the polysaccharides was evaluated by determining their radical (hydroxyl radical, superoxide anion radical and DPPH radical) scavenging abilities, and ferric iron reducing power. The inhibitory activity on tyrosinase of DPSF was also evaluated. The results indicate that the degraded polysaccharide has superior antioxidant activity and anti-tyrosinase effect to those of the original polysaccharide.
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, Xiasha, Hangzhou, Zhejiang 310018, PR China.
authors.forename: Bing-Jie
authors.initials: BJ
authors.lastname: Chen
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, Xiasha, Hangzhou, Zhejiang 310018, PR China.
authors.forename: Mei-Jia
authors.initials: MJ
authors.lastname: Shi
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, Xiasha, Hangzhou, Zhejiang 310018, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Cui
authors.affiliation: South China Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, PR China.
authors.forename: Shu-Xian
authors.initials: SX
authors.lastname: Hao
authors.affiliation: Institute of Pharmaceutical Science, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Hider
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, Xiasha, Hangzhou, Zhejiang 310018, PR China. Electronic address: taozhou@zjgsu.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D001205:Ascorbic Acid / Q000737:chemistry; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D005163:Factor Analysis, Statistical; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D006861:Hydrogen Peroxide / Q000737:chemistry; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D008970:Molecular Weight; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D010084:Oxidation-Reduction; D010851:Picrates / Q000037:antagonists & inhibitors; D044647:Sargassum / Q000737:chemistry*; D013481:Superoxides / Q000037:antagonists & inhibitors; D013696:Temperature
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D062610:Fungal Polysaccharides; D010851:Picrates; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D014442:Monophenol Monooxygenase; D001205:Ascorbic Acid
keywords: Anti-tyrosinase activity; Antioxidant activity; Polysaccharide from Sargassum fusiforme