title: Blimp-1-mediated CD4 T cell exhaustion causes CD8 T cell dysfunction during chronic toxoplasmosis.
abstract: CD8, but not CD4, T cells are considered critical for control of chronic toxoplasmosis. Although CD8 exhaustion has been previously reported in Toxoplasma encephalitis (TE)-susceptible model, our current work demonstrates that CD4 not only become exhausted during chronic toxoplasmosis but this dysfunction is more pronounced than CD8 T cells. Exhausted CD4 population expressed elevated levels of multiple inhibitory receptors concomitant with the reduced functionality and up-regulation of Blimp-1, a transcription factor. Our data demonstrates for the first time that Blimp-1 is a critical regulator for CD4 T cell exhaustion especially in the CD4 central memory cell subset. Using a tamoxifen-dependent conditional Blimp-1 knockout mixed bone marrow chimera as well as an adoptive transfer approach, we show that CD4 T cell-intrinsic deletion of Blimp-1 reversed CD8 T cell dysfunction and resulted in improved pathogen control. To the best of our knowledge, this is a novel finding, which demonstrates the role of Blimp-1 as a critical regulator of CD4 dysfunction and links it to the CD8 T cell dysfunctionality observed in infected mice. The critical role of CD4-intrinsic Blimp-1 expression in mediating CD4 and CD8 T cell exhaustion may provide a rational basis for designing novel therapeutic approaches.
authors.affiliation: Department of Microbiology, Immunology, and Tropical Medicine, George Washington University, Washington, DC 20037.
authors.forename: SuJin
authors.identifier: 0000-0001-8531-4575
authors.initials: S
authors.lastname: Hwang
authors.affiliation: Department of Microbiology, Immunology, and Tropical Medicine, George Washington University, Washington, DC 20037 Department of Microbiology, Immunology, and Cancer Biology, Beirne B. Carter Center for Immunology Research, University of Virginia, Charlottesville, VA 22908.
authors.forename: Dustin A
authors.initials: DA
authors.lastname: Cobb
authors.affiliation: Department of Microbiology, Immunology, and Tropical Medicine, George Washington University, Washington, DC 20037.
authors.forename: Rajarshi
authors.initials: R
authors.lastname: Bhadra
authors.affiliation: Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105.
authors.forename: Ben
authors.identifier: 0000-0002-1261-9957
authors.initials: B
authors.lastname: Youngblood
authors.affiliation: Department of Microbiology, Immunology, and Tropical Medicine, George Washington University, Washington, DC 20037 imit56@gwu.edu.
authors.forename: Imtiaz A
authors.identifier: 0000-0002-0663-9556
authors.initials: IA
authors.lastname: Khan
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology*; D002908:Chronic Disease; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D000074462:Positive Regulatory Domain I-Binding Factor 1; D061026:Programmed Cell Death 1 Receptor / Q000502:physiology; D014123:Toxoplasmosis / Q000276:immunology*; D014157:Transcription Factors / Q000502:physiology*
chemical_list: C491383:Pdcd1 protein, mouse; C513258:Prdm1 protein, mouse; D061026:Programmed Cell Death 1 Receptor; D014157:Transcription Factors; D000074462:Positive Regulatory Domain I-Binding Factor 1