title: Thymic Stromal Lymphopoietin Neutralization Inhibits the Immune Adjuvant Effect of Di-(2-Ethylhexyl) Phthalate in Balb/c Mouse Asthma Model.
abstract: Di-(2-ethylhexyl) phthalate (DEHP), a commonly used plasticizer, has an adjuvant effect in combination with ovalbumin (OVA). The adjuvant effect of DEHP has already been verified in our previous studies. In this study, to further investigate whether thymic stromal lymphopoietin (TSLP) was involved in the DEHP-adjuvant effect, DEHP was administered through a daily gavage exposure route. Mice were sensitized with ovalbumin (OVA) to trigger allergic responses, and an anti-TSLP monoclonal antibody was used to neutralize the effect of TSLP. Biomarkers including cytokines in bronchoalveolar lavage fluid (BALF), serum total IgE and TSLP content in the lung were detected. In addition, airway hyperreactivity and lung sections were examined. Collectively, these data indicated a salient Th2 response which was characterized by the upregulation of Th2-type cytokines, such as interleukin 4 (IL-4), IL-5 and IL-13. Moreover, the eosinophil number in BALF and the eosinophil cationic protein (ECP) in the lung were seen to have increased significantly. However, neutralization of TSLP with an anti-TSLP mAb reversed the adjuvant effect of DEHP on airway inflammation, structural alterations in the airway wall and increased airway hyperresponsiveness (AHR) to methacholine induced by the OVA allergen, suggesting that TSLP was an effective target site for suppressing the adjuvant effect of DEHP co-exposure.
authors.affiliation: Laboratory of Environmental Biomedicine, School of Life Sciences, Central China Normal University, Wuhan, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: You
authors.affiliation: Laboratory of Environmental Biomedicine, School of Life Sciences, Central China Normal University, Wuhan, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Key Laboratory of Ecological Safety Monitoring and Evaluation, College of Life Sciences, Hunan Normal University, Changsha, China.
authors.forename: Chenxi
authors.initials: C
authors.lastname: Wei
authors.affiliation: Laboratory of Environmental Biomedicine, School of Life Sciences, Central China Normal University, Wuhan, China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Chen
authors.affiliation: Laboratory of Environmental Biomedicine, School of Life Sciences, Central China Normal University, Wuhan, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Mao
authors.affiliation: University Hospital, Central China Normal University, Wuhan, China.
authors.forename: Zhenye
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Laboratory of Environmental Biomedicine, School of Life Sciences, Central China Normal University, Wuhan, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001249:Asthma / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid; D016207:Cytokines / Q000276:immunology*; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D004195:Disease Models, Animal; D007074:Immunoglobulin G / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010047:Ovalbumin / Q000008:administration & dosage; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D007074:Immunoglobulin G; D010047:Ovalbumin; D004051:Diethylhexyl Phthalate; D000094632:Thymic Stromal Lymphopoietin; C000721967:TSLP protein, mouse