title: Rare Spontaneous Loss of Multiresistance Gene Carrying IncI/ST12 Plasmid in Escherichia coli in Pig Microbiota.
abstract: Resistance to extended-spectrum cephalosporins (ESCs) is a matter of considerable concern for public health. Here, we studied the spontaneous loss of an extended-spectrum beta-lactamase (ESBL)-encoding plasmid from a rifampin-resistant Escherichia coli isolate orally inoculated into pigs under controlled conditions. Fecal samples were collected and cultured on rifampin-supplemented medium, and the resistance of the E. coli isolates to ESCs was studied by phenotypic tests, PCR detection of plasmid genes, and complete sequencing. The results showed that only 3 out of 353 rifampin-resistant E. coli isolates were ESC susceptible, and PCR and bioinformatics analysis confirmed the loss of the plasmid. These in vivo experiments indicate that the loss of an ESBL-encoding plasmid seems a rare event in gut microbiota.
authors.affiliation: Laboratoire de Ploufragan-Plouzané, Agence Nationale de Sécurité Sanitaire (Anses), Université Bretagne Loire, Ploufragan, France.
authors.forename: Gwenaelle
authors.initials: G
authors.lastname: Mourand
authors.affiliation: Laboratoire de Ploufragan-Plouzané, Agence Nationale de Sécurité Sanitaire (Anses), Université Bretagne Loire, Ploufragan, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Touzain
authors.affiliation: Laboratoire de Ploufragan-Plouzané, Agence Nationale de Sécurité Sanitaire (Anses), Université Bretagne Loire, Ploufragan, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Jouy
authors.affiliation: Laboratoire de Ploufragan-Plouzané, Agence Nationale de Sécurité Sanitaire (Anses), Université Bretagne Loire, Ploufragan, France.
authors.forename: Mickael Alain
authors.initials: MA
authors.lastname: Fleury
authors.affiliation: INSERM, IAME, UMR1137, Paris, France Université Paris Diderot, IAME, UMR1137, Sorbonne Paris Cité, Paris, France AP-HP, Laboratoire de Génétique Moléculaire, GH Paris Nord Val de Seine, Paris, France.
authors.forename: Erick
authors.initials: E
authors.lastname: Denamur
authors.affiliation: Laboratoire de Ploufragan-Plouzané, Agence Nationale de Sécurité Sanitaire (Anses), Université Bretagne Loire, Ploufragan, France isabelle.kempf@anses.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Kempf
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000737:chemistry; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression; D059014:High-Throughput Nucleotide Sequencing; D010957:Plasmids / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012293:Rifampin / Q000494:pharmacology; D017384:Sequence Deletion; D013552:Swine; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases; D012293:Rifampin