title: [Role of glucose-6-phosphate dehydrogenase in hepatitis B virus replication and its possible mechanism of action].
abstract: OBJECTIVE
  To investigate the role of glucose-6-phosphate dehydrogenase (G6PD) in hepatitis B virus (HBV) replication and its possible mechanism of action.
  
  
  METHODS
  Tissue microarray, quantitative real-time PCR, and Western blot were performed to analyze the differences in G6PD expression levels in the HBV-positive and HBV-negative liver tissues, HepG2.2.15 cells, and HepG2 cells. The siRNA transfection technique was used to knock down G6PD gene in HepG2.2.15 cells for 48 hours. Chemiluminescence was used for HBsAg and HBeAg quantification in supernatant, and quantitative real-time PCR was used to measure HBV DNA, type I interferon (IFN), and downstream IFN-stimulated genes. The t-test was used for comparison between groups.
  
  
  RESULTS
  G6PD expression was significantly upregulated in the HBV-positive liver tissues and cells compared with HBV-negative liver tissues and cells, and the stain intensity and immunohistochemical scores were 89.69±54.92 and 31.90±18.62, respectively (P < 0.05). After G6PD expression in HepG2.2.15 cells was interfered by siRNA, the quantitative levels of HBV DNA, HBsAg, and HBeAg in supernatant were reduced significantly, and the mRNA expression levels of IFNα1, IFNβ1, and five downstream IFN-stimulated genes (OAS1, ISG15, OAS3, EIF2α, and PKR) increased significantly (all P < 0.05).
  
  
  CONCLUSIONS
  G6PD plays a vital role in HBV replication, and its mechanism of action in regulating HBV replication may be related to type I IFN signaling pathway.
authors.affiliation: Department of Infectious Disease and Emergency Department, General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Chen
authors.affiliation: Department of Infectious Disease and Emergency Department, General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Guan
authors.affiliation: Department of Infectious Disease and Emergency Department, General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: L N
authors.initials: LN
authors.lastname: Ma
authors.affiliation: Department of Infectious Disease and Emergency Department, General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Liu
authors.affiliation: The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Yang
authors.affiliation: The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
authors.forename: H D
authors.initials: HD
authors.lastname: Hu
authors.affiliation: Department of Infectious Disease and Emergency Department, General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: X C
authors.initials: XC
authors.lastname: Ding
authors.affiliation: The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
authors.forename: P
authors.initials: P
authors.lastname: Hu
mesh_terms: D004279:DNA, Viral / Q000302:isolation & purification; D055785:Gene Knockdown Techniques; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D056945:Hep G2 Cells; D006509:Hepatitis B; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification; D006513:Hepatitis B e Antigens / Q000302:isolation & purification; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000821:virology; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D007370:Interferon Type I; D034741:RNA, Small Interfering; D005954:Glucosephosphate Dehydrogenase