title: Upregulation of Human ST8Sia VI (α2,8-Sialyltransferase) Gene Expression by Physcion in SK-N-BE(2)-C Human Neuroblastoma Cells.
abstract: In this research, we firstly demonstrated that physcion, an anthraquinone derivative, specifically increased the expression of the human α2,8-sialyltransferase (hST8Sia VI) gene in SK-N-BE(2)-C human neuroblastoma cells. To establish the mechanism responsible for the up-regulation of hST8Sia VI gene expression in physcion-treated SK-N-BE(2)-C cells, the putative promoter region of the hST8Sia VI gene was functionally characterized. Promoter analysis with serially truncated fragments of the 5'-flanking region showed that the region between -320 and -240 is crucial for physcion-induced transcription of hST8Sia VI in SK-N-BE(2)-C cells. Putative binding sites for transcription factors Pax-5 and NF-Y are located at this region. The Pax-5 binding site at -262 to -256 was essential for the expression of the hST8Sia VI gene by physcion in SK-N-BE(2)-C cells. Moreover, the transcription of hST8Sia VI induced by physcion in SK-N-BE(2)-C cells was inhibited by extracellular signal-regulated protein kinase (ERK) inhibitor U0126 and p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580, but not c-Jun N-terminal kinase (JNK) inhibitor SP600125. These results suggest that physcion upregulates hST8Sia VI gene expression via ERK and p38 MAPK pathways in SK-N-BE(2)-C cells.
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A university, Busan 49315, Korea. gusrud073@naver.com.
authors.forename: Hyun-Kyoung
authors.initials: HK
authors.lastname: Yoon
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A university, Busan 49315, Korea. beastne@nate.com.
authors.forename: Hyun-Kyu
authors.initials: HK
authors.lastname: An
authors.affiliation: Department of Horticultural Bioscience, Pusan National University, Miryang 50463, Korea. komj99@pusan.ac.kr.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Ko
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A university, Busan 49315, Korea. kskim@dau.ac.kr.
authors.forename: Kyoung-Sook
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A university, Busan 49315, Korea. ss11033@hanmail.net.
authors.forename: Seo-Won
authors.initials: SW
authors.lastname: Mun
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A university, Busan 49315, Korea. mose79@dau.ac.kr.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Research Center for Anti-Aging Technology Development, Pusan National University, Busan 46241, Korea. kimcm@pusan.ac.kr.
authors.forename: Cheol Min
authors.initials: CM
authors.lastname: Kim
authors.affiliation: Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Kyunggi-Do 16419, Korea. chkimbio@skku.edu.
authors.forename: Cheorl-Ho
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Department of Horticultural Bioscience, Pusan National University, Miryang 50463, Korea. ywchoi@pusan.ac.kr.
authors.forename: Young Whan
authors.initials: YW
authors.lastname: Choi
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A university, Busan 49315, Korea. yclee@dau.ac.kr.
authors.forename: Young-Choon
authors.initials: YC
authors.lastname: Lee
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D001932:Brain Neoplasms / Q000201:enzymology / Q000235:genetics*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004642:Emodin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D009447:Neuroblastoma / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D012799:Sialyltransferases / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects* / Q000235:genetics; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012799:Sialyltransferases; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C000607061:ST8SIA6 protein, human; C008905:physcione; D004642:Emodin
keywords: SK-N-BE(2)-C; hST8Sia VI; physcion; signal pathway; transcription factor Pax-5