title: Expression of Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells.
abstract: The expression of recombinant viral envelope glycoproteins in S2 (Drosophila melanogaster) has been performed with good results. This chapter contains protocols for the utilization of this system for the expression and analysis of proteins presented in cell plasma membrane.
authors.affiliation: Laboratório de Imunologia Viral, Instituto Butantan, Butantan, Brazil. renato.astray@butantan.gov.br.
authors.forename: Renato Mancini
authors.initials: RM
authors.lastname: Astray
authors.affiliation: Laboratório de Imunologia Viral, Instituto Butantan, Butantan, Brazil.
authors.forename: Sandra Fernanda
authors.initials: SF
authors.lastname: Suárez-Patiño
authors.affiliation: Laboratório de Imunologia Viral, Instituto Butantan, Butantan, Brazil.
authors.forename: Soraia Attie Calil
authors.initials: SA
authors.lastname: Jorge
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology; D015870:Gene Expression; D015202:Protein Engineering / Q000379:methods*; D011820:Rabies virus / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D014759:Viral Envelope Proteins
keywords: Drosophila melanogaster; Insect cells; S2 cells; S2 protocols; Viral envelope glycoprotein