title: LATS2 as a poor prognostic marker regulates non-small cell lung cancer invasion by modulating MMPs expression.
abstract: Large tumor suppressor 2 (LATS2) plays significant roles in tumorigenesis and cancer progression. This study was aimed to analyze the correlation between LATS2 expression and clinicopathologic features and its prognostic significance in non-small cell lung cancer (NSCLC). LATS2 expression was examined in 73 NSCLC clinical specimens and 22 normal lung tissues using immunohistochemistry. Low levels of LATS2 protein were inversely associated with the T classification (P=0.001), N classification (P=0.005) and clinical stage (P=0.001) in NSCLC patients. Patients with lower LATS2 expression had a significantly shorter overall survival than patients with high LATS2 expression. Multivariate analysis suggested that low expression of LATS2 was an independent prognostic indicator (P=0.002) for the survival of patients with NSCLC. Furthermore, overexpression of LATS2 resulted in mobility inhibition in NSCLC cell lines A549 and H1299, and reduced protein level of matrix metalloproteinase-2 (MMP-2) and MMP-9. On the contrary, LATS2 siRNA treatment enhanced cell mobility and increased MMP-2 and MMP-9 protein expression level. In conclusion, low expression of LATS2 is a potential unfavorable prognostic factor and promoted cell invasion and migration in NSCLC.
authors.affiliation: Cancer Center, Affiliated Hospital of Guangdong Medical University, No. 57 Peoples Avenue South, Zhanjiang 524001, Guangdong, China.
authors.forename: Aibing
authors.initials: A
authors.lastname: Wu
authors.affiliation: Cancer Center, Affiliated Hospital of Guangdong Medical University, No. 57 Peoples Avenue South, Zhanjiang 524001, Guangdong, China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Li
authors.affiliation: Cancer Center, Heyuan People's Hospital, No. 733 Wenxiang Road, Heyuan 517000, Guangdong, China.
authors.forename: Kunpeng
authors.initials: K
authors.lastname: Wu
authors.affiliation: Cancer Center, Affiliated Hospital of Guangdong Medical University, No. 57 Peoples Avenue South, Zhanjiang 524001, Guangdong, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Mo
authors.affiliation: Cancer Center, Affiliated Hospital of Guangdong Medical University, No. 57 Peoples Avenue South, Zhanjiang 524001, Guangdong, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Cancer Center, Affiliated Hospital of Guangdong Medical University, No. 57 Peoples Avenue South, Zhanjiang 524001, Guangdong, China.
authors.forename: Haiyin
authors.initials: H
authors.lastname: Ye
authors.affiliation: Cancer Center, Affiliated Hospital of Guangdong Medical University, No. 57 Peoples Avenue South, Zhanjiang 524001, Guangdong, China.
authors.forename: Zongjiong
authors.initials: Z
authors.lastname: Mai
authors.affiliation: Cancer Center, Affiliated Hospital of Guangdong Medical University, No. 57 Peoples Avenue South, Zhanjiang 524001, Guangdong, China.
authors.forename: Kangwen
authors.initials: K
authors.lastname: Guo
authors.affiliation: Cancer Center, Affiliated Hospital of Guangdong Medical University, No. 57 Peoples Avenue South, Zhanjiang 524001, Guangdong, China.
authors.forename: Yuzhou
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Cancer Center, Affiliated Hospital of Guangdong Medical University, No. 57 Peoples Avenue South, Zhanjiang 524001, Guangdong, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Li
authors.affiliation: Cancer Center, Affiliated Hospital of Guangdong Medical University, No. 57 Peoples Avenue South, Zhanjiang 524001, Guangdong, China.
authors.forename: Hualin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong, China; Cancer Research Institute, Southern Medical University, Guangzhou 510515, Guangdong, China. Electronic address: luoweiren@hotmail.com.
authors.forename: Weiren
authors.initials: W
authors.lastname: Luo
authors.affiliation: Cancer Center, Affiliated Hospital of Guangdong Medical University, No. 57 Peoples Avenue South, Zhanjiang 524001, Guangdong, China. Electronic address: yangzhixiong068@126.com.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Yang
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008168:Lung / Q000473:pathology; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D011379:Prognosis; D016016:Proportional Hazards Models; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; D025521:Tumor Suppressor Proteins; C406449:LATS2 protein, human; D017346:Protein Serine-Threonine Kinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: Invasion; Large tumor suppressor 2; Migration; Non-small cell lung cancer; Prognosis