title: Highly efficient production of optically pure l-lactic acid from corn stover hydrolysate by thermophilic Bacillus coagulans.
abstract: A thermophilic strain Bacillus coagulans (NBRC 12714) was employed to produce l-lactic acid from corn stover hydrolysate in membrane integrated continuous fermentation. The strain NBRC 12714 metabolized glucose and xylose by the Embden-Meyerhof-Parnas pathway (EMP) and the pentose phosphate pathway (PPP), producing l-lactic acid with optical purity >99.5%. The overall l-lactic acid titer of 92g/l with a yield of 0.91g/g and a productivity of 13.8g/l/h were achieved at a dilution rate of 0.15h(-1). The productivity obtained was 1.6-fold than that of conventional continuous fermentation without cell recycling, and also was the highest among the relevant studies ever reported. These results indicated that the process developed had great potential for economical industrial production of l-lactic acid from lignocellulosic biomass.
authors.affiliation: College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, PR China.
authors.forename: Kedong
authors.initials: K
authors.lastname: Ma
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Guoquan
authors.initials: G
authors.lastname: Hu
authors.affiliation: College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, PR China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Pan
authors.affiliation: College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, PR China.
authors.forename: Zichao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China. Electronic address: hemingxiong@caas.cn.
authors.forename: Mingxiong
authors.initials: M
authors.lastname: He
mesh_terms: D000070000:Bacillus coagulans / Q000378:metabolism*; D018533:Biomass; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D019344:Lactic Acid / Q000096:biosynthesis*; D010427:Pentose Phosphate Pathway; D014994:Xylose / Q000378:metabolism*; D003313:Zea mays
chemical_list: D019344:Lactic Acid; D014994:Xylose; D005947:Glucose
keywords: Bacillus coagulans; Lignocellulosic hydrolysates; Membrane cell-recycle; Open continuous fermentation; l-lactic acid