title: Study on the bio-methane yield and microbial community structure in enzyme enhanced anaerobic co-digestion of cow manure and corn straw.
abstract: The use of enzymes to improve anaerobic co-digestion (AcoD) of cow manure and corn straw was explored in this study, including cellulase pretreatment and direct additions of amylase and protease. The effects of enzymes on microbial community structure were investigated though PCR-DGGE method. Results showed that AcoD with amylase achieved the highest methane yield of 377.63ml·CH4/g·VS, which was an increase of 110.79%. The methane increment consumed the amylase of 4.18×10(-5)g/ml·CH4. Enzymes mainly affected the bacteria in the hydrolysis stage rather than the bacteria in the hydrogenesis and acetogenesis stage and the archaea in the methanogenesis stage. However, the experimental results demonstrated that enzymes had no negative influence on microbial communities; the predominant microbial communities were similar. Therefore, AcoD with amylase was an effective way to improve the bio-methane yield of cow manure and corn straw.
authors.affiliation: School of Energy and Environmental Engineering, Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, Beijing 100083, PR China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Energy and Environmental Engineering, Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, Beijing 100083, PR China. Electronic address: zifulee@aliyun.com.
authors.forename: Zifu
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Energy and Environmental Engineering, Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, Beijing 100083, PR China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Energy and Environmental Engineering, Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, Beijing 100083, PR China.
authors.forename: Qiqi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: University College London, UK.
authors.forename: Yanga
authors.initials: Y
authors.lastname: Wu
authors.affiliation: School of Energy and Environmental Engineering, Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, Beijing 100083, PR China.
authors.forename: Mayiani
authors.initials: M
authors.lastname: Saino
authors.affiliation: School of Energy and Environmental Engineering, Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, Beijing 100083, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Bai
mesh_terms: D000818:Animals; D001105:Archaea / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism; D018533:Biomass; D002417:Cattle; D004798:Enzymes; D005285:Fermentation; D006868:Hydrolysis; D008372:Manure / Q000382:microbiology*; D008697:Methane / Q000096:biosynthesis*; D003313:Zea mays
chemical_list: D004798:Enzymes; D008372:Manure; D008697:Methane
keywords: Anaerobic co-digestion; Enzymatic treatment; Methane yield; PCR-DGGE