title: MALAT1 promotes the proliferation and invasion of thyroid cancer cells via regulating the expression of IQGAP1.
abstract: BACKGROUND
  Increasing evidence indicated that metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) acted as a key regulator in the proliferation and invasion of several cancers. However, the function of MALAT1 in the development of thyroid cancer has not been experimentally established.
  
  
  METHODS
  The expression of MALAT1 and IQGAP1 in thyroid cancer tissues and cells were detected by quantitative real-time PCR and western blot. The effects of MALAT1 and IQGAP1 on the cell proliferation and invasion of thyroid cancer cells were detected with a 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium 4 (MTT) assay and a Transwell assay, respectively. FTC-133 or SW1736 transfected with si-MALAT1 or pcDNA-MALAT1 were injected subcutaneously into 4-week-olds BALB/c mice to examine the impact of MALAT1 on the tumor development of thyroid cancer in vivo.
  
  
  RESULTS
  In this study, we discovered the higher level of MALAT-1 and expression of IQGAP1 in thyroid cancer tissues and in thyroid cancer cells compared to that in the control. MTT and Transwell assay showed that the proliferation and invasion of FTC-133 cells with MALAT-1 knockdown were inhibited. Moreover, MALAT-1 could upregulate the expression of IQGAP1 in thyroid cancer cells. In addition, IQGAP1 knockdown reversed the decreasing cell proliferation and invasion of thyroid cancer induced by MALAT-1 overexpression. Finally, the study in vivo verified that MALAT-1 promoted the tumor growth of thyroid cancer.
  
  
  CONCLUSIONS
  Our study indicated that MALAT1 promoted the proliferation and invasion of thyroid cancer cells via regulating the expression of IQGAP1.
authors.affiliation: Department of Oncological Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Huang
authors.affiliation: Department of Gynecologic Oncology, The First Affiliated Hospital of Bengbu Medical College, No. 287, Changhuai Road, Bengbu, Anhui Province, Bengbu 233004, China. Electronic address: ml1970222@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Oncological Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Song
authors.affiliation: Department of Minimally Invasive Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
authors.forename: Bang-Yu
authors.initials: BY
authors.lastname: Lu
authors.affiliation: Department of Minimally Invasive Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
authors.forename: Yu-Bin
authors.initials: YB
authors.lastname: Huang
authors.affiliation: Department of Oncological Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Dong
authors.affiliation: Department of Oncological Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
authors.forename: Xiao-Kai
authors.initials: XK
authors.lastname: Ma
authors.affiliation: Department of Oncological Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
authors.forename: Zheng-Zhi
authors.initials: ZZ
authors.lastname: Zhu
authors.affiliation: Department of Oncological Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D040521:Subcutaneous Tissue / Q000473:pathology; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology*; D015854:Up-Regulation; D023041:Xenograft Model Antitumor Assays; D020703:ras GTPase-Activating Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C101203:IQ motif containing GTPase activating protein 1; C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding; D020703:ras GTPase-Activating Proteins
keywords: IQGAP1; Invasion; MALAT1; Proliferation; Thyroid cancer