title: A SIMPLE AND-SENSITIVE STABILITY-INDICATING UHPLC-DAD METHOD FOR THE DETERMINATION OF CEFETAMET PIVOXIL HYDROCHLORIDE.
abstract: A fast and sensitive UHPLC-DAD method was developed and subsequently validated for determination of cefetamet pivoxil hydrochloride in the presence of its degradation products. The chromatographic separation was carried out on a Waters Acquity BEH C18, (2.1 x 100 mm, 1.7 µm) column. The mobile phase was composed of 0.1% formic acid and acetonitrile (40 : 60, v/v) at the flow rate 0.7 mL/min. The detection wavelength was 265 nm and the temperature was 30 °C. Cefetamet pivoxil hydrochloride was susceptible to degradation under the influence of sodium hydroxide, hydrochloric acid and in the conditions of increased temperature and relative humidity. However, it was stable after irradiation, in increased temperature in dry air and in the presence of oxidizing agent. The developed UHPLC-DAD method was linear over the concentration range of 10-240 µg/mL (r2 = 0.9999; n = 12). The obtained RSD values were less than 2%, demonstrating that the described procedure is precise. The accuracy was also confirmed (mean recoveries were 97.79-102.08%). Under applied chromatographic conditions LOD and LOQ values were 2.08 mg/mL and 6.29 mg/mL, respectively. The proposed method was successfully applied in determination of cefetamet pivoxil hydrochloride in aqueous solutions as well as in the solid state.
authors.forename: Piotr
authors.initials: P
authors.lastname: Garbacki
authors.forename: Judyta
authors.initials: J
authors.lastname: Cielecka-Piontek
authors.forename: Przemysław
authors.initials: P
authors.lastname: Zalewski
authors.forename: Irena
authors.initials: I
authors.lastname: Oszczapowicz
authors.forename: Anna
authors.initials: A
authors.lastname: Jelińska
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D015296:Ceftizoxime / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D057230:Limit of Detection; D015203:Reproducibility of Results
chemical_list: D000900:Anti-Bacterial Agents; D015296:Ceftizoxime; C047445:cefetamet pivoxyl