title: Optimization, characterization, sulfation and antitumor activity of neutral polysaccharides from the fruit of Borojoa sorbilis cuter.
abstract: Extraction optimization, purification, characterization, sulfation and antitumor activity of polysaccharides from the fruit body of Borojoa sorbilis cuter were investigated in present study. The optimal Ultrahigh Pressure extraction condition was determined as: extraction once with the solid-liquid ratio of 1:10 in 30°C and 1500Mpa for crude polysaccharide (BP) and experimental yield was 8.28%. Four water-soluble polysaccharides named as BP1-1, BP1-2, BP1-3 and BP1-4, with molecular weight of 35.8, 32.4, 30.1 and 27.7kDa, were purified by DEAE Sepharose and Superdex 200 chromatography. On the basis of chemical and spectroscopic analyses, BP1-1-BP1-4 were found to be neutral β-d-galactan containing a (1→4)-linked backbone. S-BP1s with the DSS of 1.18, was sulfated by chloro-sulfonic acid-pyridine method. Furthermore, S-BP1s exhibited significant in vitro antitumor activity against liver cancer HepG2 and lung cancer A549 cells in a dose-dependent manner. The results indicated that S-BP1s could be potentially developed as functional antitumor drug.
authors.affiliation: The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Xu
authors.affiliation: The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China.
authors.forename: Kangsheng
authors.initials: K
authors.lastname: Liao
authors.affiliation: The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China.
authors.forename: Yunshan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Pan
authors.affiliation: The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China.
authors.forename: Lilan
authors.initials: L
authors.lastname: Wu
authors.affiliation: Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510006, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jiao
authors.affiliation: The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China; Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai 201203, China. Electronic address: daguo@simm.ac.cn.
authors.forename: Dean
authors.initials: D
authors.lastname: Guo
authors.affiliation: The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China. Electronic address: lcyxlb@126.com.
authors.forename: Ben
authors.initials: B
authors.lastname: Li
authors.affiliation: The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China. Electronic address: doctliu@263.net.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
mesh_terms: D000072283:A549 Cells; D000970:Antineoplastic Agents / Q000494:pharmacology; D002845:Chromatography / Q000379:methods; D005638:Fruit / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019666:Rubiaceae / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D013451:Sulfonic Acids
keywords: Antitumor activity; Borojoa sorbilis cuter; Polysaccharide; Structural characterization; Sulfation