title: Structural identification of the lipopolysaccharide-binding capability of a cupin-family protein from Helicobacter pylori.
abstract: We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry and NMR titration demonstrated that HP0902 is able to bind bacterial endotoxin lipopolysaccharides (LPS) through its surface-exposed loops, where metal-binding sites are usually found in other metallo-cupins. This report constitutes the first identification of an LPS-interacting protein, both in the cupin family and in H. pylori. Furthermore, identification of the ability of HP0902 to bind LPS uncovers a putative role for this protein in H. pylori pathogenicity.
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungbuk, Korea.
authors.forename: Dae-Won
authors.initials: DW
authors.lastname: Sim
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungbuk, Korea.
authors.forename: Ji-Hun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Protein Structure Group, Korea Basic Science Institute, Chungbuk, Korea.
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungbuk, Korea.
authors.forename: Jung-Hwa
authors.initials: JH
authors.lastname: Jang
authors.affiliation: Protein Structure Group, Korea Basic Science Institute, Chungbuk, Korea.|Division of Biotechnology, Korea University, Seoul, Korea.
authors.forename: Woo Cheol
authors.initials: WC
authors.lastname: Lee
authors.affiliation: Protein Structure Group, Korea Basic Science Institute, Chungbuk, Korea.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungbuk, Korea.
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungbuk, Korea.
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungbuk, Korea.
authors.forename: Hyung-Sik
authors.initials: HS
authors.lastname: Won
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D016480:Helicobacter pylori / Q000737:chemistry* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D011485:Protein Binding; D011487:Protein Conformation; D013499:Surface Properties
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C000618191:HP0902 protein, Helicobacter pylori; D008070:Lipopolysaccharides
keywords: HP0902; Helicobacter pylori; NMR spectroscopy; crystal structure; cupin superfamily; lipopolysaccharides