title: Endoplasmic Reticulum Chaperones Are Potential Active Factors in Thyroid Tumorigenesis.
abstract: The study aimed to evaluate the proteomic changes in benign follicular adenoma versus malignant follicular variant of papillary thyroid carcinoma. Tumor and nontumor adjacent samples were analyzed by liquid nanochromatography mass spectrometry, and protein abundance was evaluated by label-free quantification. Western blotting and quantitative real-time polymerase chain reaction were used to validate and complement the mass spectrometry data. The results demonstrated deregulated expression of four endoplasmic reticulum chaperones (78 kDa glucose-regulated protein, endoplasmin, calnexin, protein disulfide-isomerase A4), glutathione peroxidase 3 and thyroglobulin, all of them involved in thyroid hormone synthesis pathway. The altered tissue abundance of endoplasmic reticulum chaperones in thyroid cancer was correlated with serum expression levels. The identified proteins significantly discriminate between adenoma and carcinoma in both thyroid tissue and corresponding sera. Data are available via ProteomeXchange with identifier PXD004322.
authors.affiliation: Institute of Cellular Biology and Pathology "Nicolae Simionescu" , 8, B.P. Hasdeu Street, P.O. Box 35-14, 050568 Bucharest, Romania.
authors.forename: Elena
authors.initials: E
authors.lastname: Uyy
authors.affiliation: Institute of Cellular Biology and Pathology "Nicolae Simionescu" , 8, B.P. Hasdeu Street, P.O. Box 35-14, 050568 Bucharest, Romania.
authors.forename: Viorel I
authors.initials: VI
authors.lastname: Suica
authors.affiliation: Institute of Cellular Biology and Pathology "Nicolae Simionescu" , 8, B.P. Hasdeu Street, P.O. Box 35-14, 050568 Bucharest, Romania.
authors.forename: Raluca M
authors.initials: RM
authors.lastname: Boteanu
authors.affiliation: National Institute of Endocrinology "C.I. Parhon" , 34-36 Aviatorilor Boulevard, 011863 Bucharest, Romania.
authors.forename: Dana
authors.initials: D
authors.lastname: Manda
authors.affiliation: National Institute of Endocrinology "C.I. Parhon" , 34-36 Aviatorilor Boulevard, 011863 Bucharest, Romania.|University of Bucharest, Faculty of Physics , 405 Atomiştilor Street, 077125 Magurele, Romania.
authors.forename: Ancuta E
authors.initials: AE
authors.lastname: Baciu
authors.affiliation: National Institute of Endocrinology "C.I. Parhon" , 34-36 Aviatorilor Boulevard, 011863 Bucharest, Romania.
authors.forename: Corin
authors.initials: C
authors.lastname: Badiu
authors.affiliation: Institute of Cellular Biology and Pathology "Nicolae Simionescu" , 8, B.P. Hasdeu Street, P.O. Box 35-14, 050568 Bucharest, Romania.
authors.forename: Felicia
authors.initials: F
authors.lastname: Antohe
mesh_terms: D000236:Adenoma / Q000737:chemistry / Q000175:diagnosis; D053898:Biosynthetic Pathways; D063646:Carcinogenesis / Q000737:chemistry*; D002277:Carcinoma / Q000737:chemistry / Q000175:diagnosis; D002853:Chromatography, Liquid; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D018832:Molecular Chaperones / Q000032:analysis* / Q000097:blood; D040901:Proteomics / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D013963:Thyroid Hormones / Q000096:biosynthesis; D013964:Thyroid Neoplasms / Q000737:chemistry* / Q000175:diagnosis
chemical_list: D018832:Molecular Chaperones; D013963:Thyroid Hormones
keywords: endoplasmic reticulum chaperone; mass spectrometry; thyroid cancer biomarkers