title: LrABCF1, a GCN-type ATP-binding cassette transporter from Lilium regale, is involved in defense responses against viral and fungal pathogens.
abstract: The L. regale ATP-binding cassette transporter gene, LrABCF1 belonging to GCN subfamily, functions as a positive regulator of plant defense against Cucumber mosaic virus, Tobacco rattle virus , and Botrytis cinerea in petunia. ATP-binding cassette (ABC) transporters are essential for membrane translocation in diverse biological processes, such as plant development and defense response. Here, a general control non-derepressible (GCN)-type ABC transporter gene, designated LrABCF1, was identified from Cucumber mosaic virus (CMV)-induced cDNA library of L. regale. LrABCF1 was up-regulated upon inoculation with CMV and Lily mottle virus (LMoV). Salicylic acid (SA) and ethylene (ET) application and treatments with abiotic stresses such as cold, high salinity, and wounding increased the transcript abundances of LrABCF1. Constitutive overexpression of LrABCF1 in petunia (Petunia × hybrida) resulted in an impairment of plant growth and development. LrABCF1 overexpression conferred reduced susceptibility to CMV, Tobacco rattle virus (TRV), and B. cinerea infection in transgenic petunia plants, accompanying by elevated transcripts of PhGCN2 and a few defense-related genes in SA-signaling pathway. Our data indicate that LrABCF1 positively modulates viral and fungal resistance.
authors.affiliation: College of Landscape Architecture and Arts, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Daoyang
authors.initials: D
authors.lastname: Sun
authors.affiliation: College of Landscape Architecture and Arts, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Xinguo
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Landscape Architecture and Arts, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Li
authors.affiliation: Crops Pathology and Genetic Research Unit, United States Department of Agriculture, Agricultural Research Service, Davis, CA, 95616, USA. cjiang@ucdavis.edu.|Department of Plant Sciences, University of California at Davis, Davis, CA, 95616, USA. cjiang@ucdavis.edu.
authors.forename: Cai-Zhong
authors.initials: CZ
authors.lastname: Jiang
authors.affiliation: College of Landscape Architecture and Arts, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. zhangyanlong@nwsuaf.edu.cn.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Landscape Architecture and Arts, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. niulixin@nwsuaf.edu.cn.
authors.forename: Lixin
authors.initials: L
authors.lastname: Niu
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000502:physiology*; D020171:Botrytis; D017799:Cucumovirus; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D027762:Lilium / Q000382:microbiology / Q000502:physiology* / Q000821:virology; D057865:Plant Immunity / Q000502:physiology*; D010942:Plant Viruses
chemical_list: D018528:ATP-Binding Cassette Transporters
keywords: ABC transporter; Botrytis cinerea; Cucumber mosaic virus; Lily mottle virus; Tobacco rattle virus; Wild Lilium