title: Association of Polymorphisms in TRAIL and Chronic Hepatitis B in Chinese Han Populations from Shandong Province.
abstract: BACKGROUND
  HBV is the most common and serious infectious disease in China. Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) belongs to the TNF-super family and can induce apoptosis in addition to other biological effects. It has been found that the occurrence of chronic hepatitis B is related to polymorphisms of many apoptotic factors. This study therefore aims to investigate the association of TRAIL polymorphism with chronic hepatitis B by observing the polymorphism and gene frequency of the TRAIL gene at 1525G/A and 1595C/T of the 3'-UTR among people of the Chinese Han ethnicity.
  
  
  METHODS
  This study followed a case-control design. Polymorphisms of the TRAIL gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods in 187 patients with chronic hepatitis B and 142 healthy controls.
  
  
  RESULTS
  The alleles of TRAIL at 1525G/A and 1595C/T have achieved genetic equilibrium in normal individuals (P<0.05) and patients with chronic hepatitis B(P=0.547). The genotype frequencies of TRAIL at 1525GG/1595CC in female patients were significantly higher than those in male patients. The frequencies of the AA genotype at 1525G/A and the TT genotype at 1595C/T sites were significantly lower in patients with chronic hepatitis B with HBeAg positive than in healthy controls (10.5 vs 23.2%, P<0.05). The frequency of the AA genotype at 1525G/A and TT genotype at 1595C/T sites were significantly lower in the chronic hepatitis B individuals than in normal controls (12.3 vs 23.2%, P < 0.05).
  
  
  CONCLUSIONS
  TRAIL may be related to susceptibility to hepatitis B.
authors.affiliation: Department of Clinical Laboratory, The Second Hospital, Shandong University, Jinan, Shandong, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Jinan Blood Center, Jinan, Shandong, China.
authors.forename: Xueliang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Traditional Chinese Medical Hospital, Rongchen, Shandong, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, The Second Hospital, Shandong University, Jinan, Shandong, China szhsdey@163.com.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006513:Hepatitis B e Antigens / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics*
chemical_list: D006513:Hepatitis B e Antigens; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human
keywords: TRAIL; Tumor necrosis factor (TNF)-related apoptosis-inducing ligand; chronic hepatitis B; gene polymorphism