title: Releasing intracellular product to prepare whole cell biocatalyst for biosynthesis of Monascus pigments in water-edible oil two-phase system.
abstract: Selective releasing intracellular product in Triton X-100 micelle aqueous solution to prepare whole cell biocatalyst is a novel strategy for biosynthesis of Monascus pigments, in which cell suspension culture exhibits some advantages comparing with the corresponding growing cell submerged culture. In the present work, the nonionic surfactant Triton X-100 was successfully replaced by edible plant oils for releasing intracellular Monascus pigments. High concentration of Monascus pigments (with absorbance nearly 710 AU at 470 nm in the oil phase, normalized to the aqueous phase volume approximately 142 AU) was achieved by cell suspension culture in peanut oil-water two-phase system. Furthermore, the utilization of edible oil as extractant also fulfills the demand for application of Monascus pigments as natural food colorant.
authors.affiliation: School of Pharmacy, State Key Laboratory of Microbial Metabolism, and Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Minglue
authors.initials: M
authors.lastname: Hu
authors.affiliation: School of Life Science and Biotechnology, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Xuehong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, State Key Laboratory of Microbial Metabolism, and Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, Shanghai Jiao Tong University, Shanghai, 200240, China. zlwang@sjtu.edu.cn.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Wang
mesh_terms: D005505:Food Coloring Agents / Q000378:metabolism*; D032741:Monascus / Q000254:growth & development*; D017830:Octoxynol / Q000494:pharmacology*; D010860:Pigments, Biological / Q000096:biosynthesis*; D010938:Plant Oils / Q000494:pharmacology*
chemical_list: D005505:Food Coloring Agents; D010860:Pigments, Biological; D010938:Plant Oils; D017830:Octoxynol
keywords: Cell suspension culture; Edible oil; Monascus pigments; Releasing intracellular product; Whole cell biocatalyst