title: Environmental metabarcoding reveals contrasting microbial communities at two poplar phytomanagement sites.
abstract: The aim of the present study is to deepen the current understanding of the microbial communities at two poplar phytomanagement sites to reveal the environmental factors that drive the abundance, diversity and composition of microbial communities. A soil analysis revealed that the two soils displayed contrasting physico-chemical characteristics, with significant lower pH and higher Cd, Zn and Mn CaCl2-extractable fractions at Leforest site, compared with Pierrelaye site. The fungal and bacterial community profiles in the poplar roots and soils were assessed through Illumina MiSeq sequencing. Diversity indices and β-diversity measures illustrated that the root microbial communities were well separated from the soil microbial communities at both sites. A detailed study of the fungal composition showed that Ascomycota dominated the overall fungal communities on poplar soil, the root samples at Pierrelaye, and the unplanted soil at the experimental sites. Conversely, Basidiomycota accounted for a much higher percentage of the fungal community in poplar root samples from the Leforest site. The root bacterial communities were dominated by Alphaproteobacteria and Actinobacteria, and the soil samples were dominated by Alphaproteobacteria and Acidobacteria. The occurrence and dominance of the ectomycorrhizal community at Leforest but not at Pierrelaye is the major feature of our data set. Overall, ectomycorrhizal root symbionts appeared to be highly constrained by soil characteristics at the phytomanagement sites. Our data support the view that mycorrhizal inoculation is needed in highly stressed and nutrient-poor environments.
authors.affiliation: Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Foulon
authors.affiliation: Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Zappelini
authors.affiliation: Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Durand
authors.affiliation: Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Valot
authors.affiliation: Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Girardclos
authors.affiliation: CNRS, LIEC UMR7360, Faculté des Sciences et Technologies, BP70239, 54506 Vandoeuvre-lès-Nancy, France; Université de Lorraine, LIEC UMR7360, Faculté des Sciences et Technologies, BP 70239, 54506 Vandoeuvre-lès-Nancy, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Blaudez
authors.affiliation: Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France; Université de Lorraine, Faculté des Sciences et Technologies, BP 70239, 54506 Vandoeuvre-les-Nancy, France. Electronic address: michel.chalot@univ-fcomte.fr.
authors.forename: Michel
authors.initials: M
authors.lastname: Chalot
mesh_terms: D001419:Bacteria / Q000145:classification*; D001673:Biodegradation, Environmental; D058893:DNA Barcoding, Taxonomic; D005602:France; D005658:Fungi / Q000145:classification*; D064307:Microbiota; D038821:Mycorrhizae; D032107:Populus / Q000254:growth & development; D012988:Soil Microbiology; D064091:Waste Disposal Facilities
keywords: Bacterial 16S; Fungal ITS; Illumina MiSeq sequencing; Phytomanagement; Poplar plantation; Trace element