title: Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay.
abstract: One of the most commonly used approaches for determining the quantity of infectious RSV particles in a given sample is the plaque assay. RSV infectious particles can be quantified by various direct and indirect methods. Here, we explain two simple methods for RSV titration: plaque assay and immunostaining assay.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Animal Health Research Center, University of Georgia, 111 Carlton Street, Athens, GA, 30602, USA.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Jorquera
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Animal Health Research Center, University of Georgia, 111 Carlton Street, Athens, GA, 30602, USA. ratripp@uga.edu.
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Tripp
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism*; D045744:Cell Line, Tumor / Q000821:virology; D002522:Chlorocebus aethiops; D006801:Humans; D007118:Immunoassay; D012136:Respiratory Syncytial Viruses / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity; D014709:Vero Cells / Q000821:virology; D010948:Viral Plaque Assay / Q000379:methods*; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral
keywords: F protein; G protein; HEp-2 cells; Immunostaining; Plaque assay; RSV; VERO cells