title: Analysis of Somatropin by Double-Injection Capillary-Zone Electrophoresis in Polybrene/Chondroitin Sulfate A Double-Coated Capillaries.
abstract: Purity determination of somatropin as a recombinant protein is important to ensure its safety and quality. This is carried out by capillary zone electrophoresis in double-injection mode using polybrene/chondroitin sulfate A double-coated capillaries. Modification of the capillary wall eliminates protein-wall interactions which results in improved accuracy and precision of the determinations. In the double-injection mode two somatropin samples are analyzed within a single electrophoretic run. Prior to the second injection, the first injected plug is electrophoresed for a predetermined time period in order to adjust the inter-plug distance. Here, the principle for the separation of somatropin charge variants is described.
authors.affiliation: Medical Products Agency (MPA), Box 26, Dag Hammarskjölds väg 42, Uppsala, 751 03, Sweden. ahmad.amini@mpa.se.|Division of Analytical Pharmaceutical Chemistry, Biomedical Centre, Uppsala University, Box 574, Uppsala, 751 23, Sweden. ahmad.amini@mpa.se.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Amini
mesh_terms: D002809:Chondroitin Sulfates / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006583:Hexadimethrine Bromide / Q000737:chemistry; D019382:Human Growth Hormone / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D019382:Human Growth Hormone; D006583:Hexadimethrine Bromide; D002809:Chondroitin Sulfates
keywords: Double-injection capillary-zone electrophoresis (DICZE); Partial electrophoresis time period (t PE); Polybrene/chondroitin sulfate A (PB/CA) double-coated capillary; Somatropin