title: TBC1D20 mediates autophagy as a key regulator of autophagosome maturation.
abstract: In humans, loss of TBC1D20 (TBC1 domain family, member 20) protein function causes Warburg Micro syndrome 4 (WARBM4), an autosomal recessive disorder characterized by congenital eye, brain, and genital abnormalities. TBC1D20-deficient mice exhibit ocular abnormalities and male infertility. TBC1D20 is a ubiquitously expressed member of the family of GTPase-activating proteins (GAPs) that increase the intrinsically slow GTP-hydrolysis rate of small RAB-GTPases when bound to GTP. Biochemical studies have established TBC1D20 as a GAP for RAB1B and RAB2A. However, the cellular role of TBC1D20 still remains elusive, and there is little information about how the functional loss of TBC1D20 causes clinical manifestations in WARBM4-affected children. Here we evaluate the role of TBC1D20 in cells carrying a null mutant allele, as well as TBC1D20-deficient mice, which display eye and testicular abnormalities. We demonstrate that TBC1D20, via its RAB1B GAP function, is a key regulator of autophagosome maturation, a process required for maintenance of autophagic flux and degradation of autophagic cargo. Our results provide evidence that TBC1D20-mediated autophagosome maturation maintains lens transparency by mediating the removal of damaged proteins and organelles from lens fiber cells. Additionally, our results show that in the testes TBC1D20-mediated maturation of autophagosomes is required for autophagic flux, but is also required for the formation of acrosomes. Furthermore TBC1D20-deficient mice, while not mimicking severe developmental brain abnormalities identified in WARBM4 affected children, display disrupted neuronal autophagic flux resulting in adult-onset motor dysfunction. In summary, we show that TBC1D20 has an essential role in the maturation of autophagosomes and a defect in TBC1D20 function results in eye, testicular, and neuronal abnormalities in mice implicating disrupted autophagy as a mechanism that contributes to WARBM4 pathogenesis.
authors.affiliation: a Department of Cell Biology, Neurobiology, and Anatomy , Human and Molecular Genetics Center, Medical College of Wisconsin , Milwaukee , WI , USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Sidjanin
authors.affiliation: a Department of Cell Biology, Neurobiology, and Anatomy , Human and Molecular Genetics Center, Medical College of Wisconsin , Milwaukee , WI , USA.
authors.forename: Anna K
authors.initials: AK
authors.lastname: Park
authors.affiliation: a Department of Cell Biology, Neurobiology, and Anatomy , Human and Molecular Genetics Center, Medical College of Wisconsin , Milwaukee , WI , USA.
authors.forename: Adam
authors.initials: A
authors.lastname: Ronchetti
authors.affiliation: b Microbiology and Molecular Genetics, Human and Molecular Genetics Center, Medical College of Wisconsin , Milwaukee , WI , USA.
authors.forename: Jamaria
authors.initials: J
authors.lastname: Martins
authors.affiliation: c Department of Microbiology and Immunology , University of Maryland School of Medicine , Baltimore , MD , USA.
authors.forename: William T
authors.initials: WT
authors.lastname: Jackson
mesh_terms: D000177:Acrosome / Q000378:metabolism / Q000473:pathology; D000818:Animals; D000071182:Autophagosomes / Q000378:metabolism* / Q000648:ultrastructure; D001343:Autophagy; D002386:Cataract / Q000378:metabolism / Q000473:pathology; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D007908:Lens, Crystalline / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008954:Models, Biological; D009043:Motor Activity; D013737:Testis / Q000473:pathology; D054875:Ubiquitination; D020761:rab1 GTP-Binding Proteins / Q000172:deficiency / Q000378:metabolism*
chemical_list: C586297:TBC1D20 protein, mouse; D020761:rab1 GTP-Binding Proteins
keywords: GAPs; GEFs; RAB GTPase; RAB1B; TBC domain; TBC1D20; Warburg micro syndrome; acrosome; autophagic flux; autophagosome; brain; cataracts; infertility; lens; lens fiber cells; lysosome; neurodegeneration; neuron; polymicrogyria; testis