title: Novel electrochemiluminescence of perylene derivative and its application to mercury ion detection based on a dual amplification strategy.
abstract: In this paper, a novel covalently crosslinked perylene derivative (PTC-PEI) composed of polyethylenimine (PEI) and perylenetetracarboxylic acid (PTCA) has been first investigated for cathodic electrochemiluminescence (ECL) in an aqueous system with dissolved O2 as coreactant. The promising novel ECL materials of PTC-PEI exhibited admirable physical and chemical stability and high ECL intensity, which held an alternative way to construct ECL sensor with improved sensitivity. Thus, it was applied to construct a dual amplified "signal-on" mercury ion (Hg(2+)) sensor by the employment of nicking endonuclease (NEase)-assisted target recycling and rolling circle amplification (RCA) for signal amplification. Herein, a long G-rich sequence was generated by RCA process to capture abundant hemin on the electrode surface, and then a significantly amplified ECL signal of PTC-PEI was obtained. Based on dual signal amplification strategy, the devised sensor showed a linear range from 0.1pM to 0.1μΜ with a detection limit down to 33fM (S/N=3), and was successfully used in the direct detection of real water sample with high sensitivity and selectivity.
authors.affiliation: The Key Laboratory of Eco-Environments in the Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715 PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: The Key Laboratory of Eco-Environments in the Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715 PR China.
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Lei
authors.affiliation: The Key Laboratory of Eco-Environments in the Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715 PR China.
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Chai
authors.affiliation: The Key Laboratory of Eco-Environments in the Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715 PR China. Electronic address: yuanruo@swu.edu.cn.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.affiliation: The Key Laboratory of Eco-Environments in the Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715 PR China. Electronic address: yingzhuo@swu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
mesh_terms: D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007477:Ions / Q000032:analysis / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation*; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry*; D010569:Perylene / Q000031:analogs & derivatives* / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007477:Ions; D010569:Perylene; D008628:Mercury; D010100:Oxygen
keywords: Dual signal amplification strategy; Electrochemiluminescence; Hemin; Mercury ion; Perylene derivative