title: Comparative proteomic analysis of the shoot apical meristem in maize between a ZmCCT-associated near-isogenic line and its recurrent parent.
abstract: The ZmCCT, one of the most important genes affecting photoperiod response, delays flowering under long-day conditions in maize (Zea mays). In this study we used the isobaric tags for relative and absolute quantification (iTRAQ) technique-based proteomics approach to identify differentially expressed proteins between a near-isogenic line (NIL) and its recurrent parent, contrasting in alleles of ZmCCT. A total of 5,259 distinct proteins were identified. Among them, 386 proteins were differentially expressed between NIL-cml line (ZmCCT-positive) and H4 line (ZmCCT-negative). Functional categorization showed that the differentially proteins were mainly involved in energy production, photosynthesis, signal transduction, and cell organization and biogenesis. Our results showed that during shoot apical meristem (SAM) development cell division proteins, carbohydrate metabolism-related proteins, and flower inhibition-related proteins were more abundant in the ZmCCT-positive line than the ZmCCT-negative line. These results, taken together with morphological observations, showed that the effect of ZmCCT on flowering might be caused by its effect on one or all of these biological processes. Although the exact roles of these putative related proteins remain to be examined, our results obtained using the proteomics approach lead to a better understanding of the photoperiodicity mechanism in maize plants.
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.|Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China.
authors.forename: Liuji
authors.initials: L
authors.lastname: Wu
authors.affiliation: Crop Designing Center, Henan Academy of Agricultural Science, Zhengzhou 450002, China.
authors.forename: Xintao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.|Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China.
authors.forename: Shunxi
authors.initials: S
authors.lastname: Wang
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.|Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China.
authors.forename: Liancheng
authors.initials: L
authors.lastname: Wu
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.|Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tian
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.|Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.|Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.|Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Chen
mesh_terms: D018519:Meristem / Q000737:chemistry* / Q000254:growth & development; D010940:Plant Proteins / Q000032:analysis*; D018520:Plant Shoots / Q000737:chemistry* / Q000254:growth & development; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D003313:Zea mays / Q000737:chemistry* / Q000254:growth & development
chemical_list: D010940:Plant Proteins; D020543:Proteome