title: Centrosomal Protein of 55 Regulates Glucose Metabolism, Proliferation and Apoptosis of Glioma Cells via the Akt/mTOR Signaling Pathway.
abstract: BACKGROUND
  Glioma is one of the most common and most aggressive brain tumors in humans. The molecular and cellular mechanisms responsible for the onset and the progression of glioma are elusive and controversial. Centrosomal protein of 55 (CEP55) was initially described as a highly coiled-coil protein that plays critical roles in cell division, but was recently identified as being overexpressed in many human cancers. The function of CEP55 has not previously been characterized in glioma. We aim to discover the effect and mechanism of CEP55 in glioma development.
  
  
  METHODS
  qRT-PCR and immunohistochemistry were used to analyze CEP55 expression. Glucose uptake, western blot, MTS, CCK-8, Caspase-3 activity and TUNEL staining assays were performed to investigate the role and mechanism of CEP55 on glioma cell process.
  
  
  RESULTS
  We found that the levels of CEP55 expression were upregulated in glioma. In addition, CEP55 appeared to regulate glucose metabolism of glioma cells. Furthermore, knockdown of CEP55 inhibited cell proliferation and induced cell apoptosis in glioma. Finally, we provided preliminary evidence that knockdown of CEP55 inhibited glioma development via suppressing the activity of Akt/mTOR signaling.
  
  
  CONCLUSIONS
  Our results demonstrated that CEP55 regulates glucose metabolism, proliferation and apoptosis of glioma cells via the Akt/mTOR signaling pathway, and its promotive effect on glioma tumorigenesis can be a potential target for glioma therapy in the future.
authors.affiliation: 1. Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China;; 2. Department of Medical Service Management, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China;
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Wang
authors.affiliation: 3. Department of Gastroenterology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China;
authors.forename: Mingna
authors.initials: M
authors.lastname: Liu
authors.affiliation: 1. Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China;
authors.forename: Hongjun
authors.initials: H
authors.lastname: Wang
authors.affiliation: 4. Department of Pathology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China;
authors.forename: Shan
authors.initials: S
authors.lastname: Yu
authors.affiliation: 5. Department of Neurosurgery, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China;
authors.forename: Zhenfeng
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: 1. Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China;
authors.forename: Jiahang
authors.initials: J
authors.lastname: Sun
authors.affiliation: 6. School of Computer and Information Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150086, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Han
authors.affiliation: 7. Division of Growth and Development, Section of Orthodontics, School of Dentistry, University of California, Los Angeles, California 90095, USA.
authors.forename: Jia
authors.initials: J
authors.lastname: Shen
authors.affiliation: 5. Department of Neurosurgery, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China;
authors.forename: Minwei
authors.initials: M
authors.lastname: Zhu
authors.affiliation: 5. Department of Neurosurgery, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China;
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Lin
authors.affiliation: 1. Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China;
authors.forename: Chuanlu
authors.initials: C
authors.lastname: Jiang
authors.affiliation: 1. Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China;
authors.forename: Mian
authors.initials: M
authors.lastname: Guo
keywords: Akt; CEP55; Glioma; apoptosis.; mTOR; proliferation