title: Electrospun silk fibroin/poly (L-lactide-ε-caplacton) graft with platelet-rich growth factor for inducing smooth muscle cell growth and infiltration.
abstract: The construction of a smooth muscle layer for blood vessel through electrospinning method plays a key role in vascular tissue engineering. However, smooth muscle cells (SMCs) penetration into the electrospun graft to form a smooth muscle layer is limited due to the dense packing of fibers and lack of inducing factors. In this paper, silk fibroin/poly (L-lactide-ε-caplacton) (SF/PLLA-CL) vascular graft loaded with platelet-rich growth factor (PRGF) was fabricated by electrospinning. The in vitro results showed that SMCs cultured in the graft grew fast, and the incorporation of PRGF could induce deeper SMCs infiltrating compared to the SF/PLLA-CL graft alone. Mechanical properties measurement showed that PRGF-incorporated graft had proper tensile stress, suture retention strength, burst pressure and compliance which could match the demand of native blood vessel. The success in the fabrication of PRGF-incorporated SF/PLLA-CL graft to induce fast SMCs growth and their strong penetration into graft has important application for tissue-engineered blood vessels.
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China;
authors.forename: Anlin
authors.initials: A
authors.lastname: Yin
authors.affiliation: Department of Biomedical Engineering, University of Memphis, Memphis, TN 38152, USA.
authors.forename: Gary L
authors.initials: GL
authors.lastname: Bowlin
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China;
authors.forename: Rifang
authors.initials: R
authors.lastname: Luo
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China;
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China;
authors.forename: Yunbing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
keywords: PRGF; electrospinning; smooth muscle cell infiltration; vascular graft