title: Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells.
abstract: In this study, we investigated the involvement of Wnt signaling in sphingosine-1-phosphate (S1P)-enhanced osteogenic differentiation of C3H10T1/2 pluripotent stem cells. We found that S1P enhanced the expression of Wnt5a and low-density lipoprotein receptor-related protein 5 or 6 (LRP5/6) during osteogenic differentiation. Wnt5a-neutralizing antibody inhibited S1P-enhanced expression of LRP5/6 and alkaline phosphatase, which are essential for osteogenic differentiation. Conversely, S1P did not affect endogenous canonical Wnt signaling. Taken together, S1P-enhanced Wnt5a promotes LRP5/6 expression, resulting in the trigger of osteogenic differentiation of C3H10T1/2 cells. These findings suggest a potential beneficial role for S1P in bone regeneration.
authors.affiliation: Periodontal Section, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hashimoto
authors.affiliation: Periodontal Section, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: Periodontal Section, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan. matsuzaki@college.fdcnet.ac.jp.|Section of Operative Dentistry and Endodontology, Department of Odontology, Fukuoka Dental College, Fukuoka, Japan. matsuzaki@college.fdcnet.ac.jp.
authors.forename: Etsuko
authors.initials: E
authors.lastname: Matsuzaki
authors.affiliation: Periodontal Section, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Higashi
authors.affiliation: Department of Clinical Pharmacology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Fumi
authors.initials: F
authors.lastname: Takahashi-Yanaga
authors.affiliation: Periodontal Section, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Takano
authors.affiliation: Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Hirata
authors.affiliation: Periodontal Section, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
authors.forename: Fusanori
authors.initials: F
authors.lastname: Nishimura
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D060492:Low Density Lipoprotein Receptor-Related Protein-5 / Q000096:biosynthesis; D060488:Low Density Lipoprotein Receptor-Related Protein-6 / Q000096:biosynthesis; D008246:Lysophospholipids / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008809:Mice, Inbred C3H; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D060449:Wnt Signaling Pathway / Q000187:drug effects; D000071818:Wnt-5a Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C501127:Dkk1 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D060492:Low Density Lipoprotein Receptor-Related Protein-5; D060488:Low Density Lipoprotein Receptor-Related Protein-6; C555483:Lrp5 protein, mouse; C555476:Lrp6 protein, mouse; D008246:Lysophospholipids; D000071818:Wnt-5a Protein; C438615:Wnt5a protein, mouse; C060506:sphingosine 1-phosphate; D013110:Sphingosine
keywords: MSC differentiation; S1P; Wnt signaling; osteoblast