title: Recombinant Newcastle disease virus expressing P53 demonstrates promising antitumor efficiency in hepatoma model.
abstract: BACKGROUND
  Numerous studies have demonstrated that the NDV-mediated gene therapy is a promising new approach for treatment of cancers. P53 plays a vital role in tumor suppression and surveillance. Therefore, we hypothesize that a recombinant NDV expressing P53 would be an ideal agent for the hepatoma therapy.
  
  
  RESULTS
  In the essay, the human P53 gene was incorporated into the genome of a lentogenic strain (named rNDV-P53), which did not affect viral replication kinetics and magnitude in HepG2 cells. Compared to the vehicle virus, rNDV-P53 increased cell growth suppressor ratio and early apoptosis by 2 folds, and decreased the mitochondrial membrane potential in HepG2 cells. In vivo studies, treatment with rNDV-P53 reduced tumor volume of tumor-bearing mice by more than 4 folds, tumor weight by more than 5 folds comparing with rNDV. The 120-day survival rate of rNDV-P53-treated mice was 75 %, survival rate of rNDV-treated mice was 12.5 %. TUNEL analysis showed a significant increase in the apoptosis rate in the tumor tissues of rNDV-P53-treated mice than that of rNDV-treated mice. Moreover, serum chemistries revealed an insignificant change of blood urea nitrogen (BUN), creatinine levels, alanine aminotransferase (ALT) and aspartate transaminase (AST) in rNDV-P53-treated group compared to normal mice, suggesting treatment with the recombinant virus was not toxic.
  
  
  CONCLUSIONS
  rNDV-P53 is a potent candidate for carcinoma therapy especially for hepatocarcinoma.
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: An
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China.
authors.forename: Tianyan
authors.initials: T
authors.lastname: Liu
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China.
authors.forename: Jinjiao
authors.initials: J
authors.lastname: He
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China.
authors.forename: Hongsong
authors.initials: H
authors.lastname: Wu
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China.
authors.forename: Yunye
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China.
authors.forename: Yunzhou
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Guo
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China.
authors.forename: Jiechao
authors.initials: J
authors.lastname: Yin
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China. deshanli@163.com.|Key Laboratory of Agricultural Biological Functional Gene, Northeast Agricultural University, Harbin, 150030, China. deshanli@163.com.
authors.forename: Deshan
authors.initials: D
authors.lastname: Li
authors.affiliation: Biopharmaceutical Lab, College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang district, Harbin, People's Republic of China. renguiping@126.com.|Key Laboratory of Agricultural Biological Functional Gene, Northeast Agricultural University, Harbin, 150030, China. renguiping@126.com.
authors.forename: Guiping
authors.initials: G
authors.lastname: Ren
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D015870:Gene Expression; D015316:Genetic Therapy; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009522:Newcastle disease virus / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: Anti-tumor; Gene therapy; P53; Recombinant NDV