title: Amperometric IFN-γ immunosensors with commercially fabricated PCB sensing electrodes.
abstract: Lab-on-a-Chip (LoC) technology has the potential to revolutionize medical Point-of-Care diagnostics. Currently, considerable research efforts are focused on innovative production technologies that will make commercial upscaling of lab-on-chip products financially viable. Printed circuit board (PCB) manufacturing techniques have several advantages in this field. In this paper we focus on transferring a complete IFN-γ enzyme-linked immune-sorbent assay (ELISA) onto a commercial PCB electrochemical biosensing platform, We adapted a commercially available ELISA to detect the enzyme product TMB/H2O2 using amperometry, successfully reproducing the colorimetry-obtained ELISA standard curve. The results demonstrate the potential for the integration of these components into an automated, disposable, electronic ELISA Lab-on-PCB diagnostic platform.
authors.affiliation: Nanoelectronics and Nanotechnology Research Group, Southampton Nanofabrication Centre, Electronics and Computer Science, University of Southampton, Southampton SO17, 1BJ UK. Electronic address: D.Moschou@soton.ac.uk.
authors.forename: Despina
authors.initials: D
authors.lastname: Moschou
authors.affiliation: Centre for Immunology and Vaccinology, Division of Infectious Diseases, Department of Medicine, Imperial College London, London, UK; Infection and Immunity, Imperial College NHS Trust, Charing Cross Hospital, London, UK.
authors.forename: Louise
authors.initials: L
authors.lastname: Greathead
authors.affiliation: Centre for Immunology and Vaccinology, Division of Infectious Diseases, Department of Medicine, Imperial College London, London, UK; Infection and Immunity, Imperial College NHS Trust, Charing Cross Hospital, London, UK.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Pantelidis
authors.affiliation: Centre for Immunology and Vaccinology, Division of Infectious Diseases, Department of Medicine, Imperial College London, London, UK; Infection and Immunity, Imperial College NHS Trust, Charing Cross Hospital, London, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Kelleher
authors.affiliation: Nanoelectronics and Nanotechnology Research Group, Southampton Nanofabrication Centre, Electronics and Computer Science, University of Southampton, Southampton SO17, 1BJ UK.
authors.forename: Hywel
authors.initials: H
authors.lastname: Morgan
authors.affiliation: Nanoelectronics and Nanotechnology Research Group, Southampton Nanofabrication Centre, Electronics and Computer Science, University of Southampton, Southampton SO17, 1BJ UK.
authors.forename: Themistoklis
authors.initials: T
authors.lastname: Prodromakis
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation; D003124:Colorimetry / Q000295:instrumentation; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004581:Electronics / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D007371:Interferon-gamma / Q000097:blood* / Q000276:immunology*; D056656:Lab-On-A-Chip Devices; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007371:Interferon-gamma
keywords: Amperometric biosensor; Immunosensors; Lab-on-PCB; PCB sensors