title: Tendon and ligament as novel cell sources for engineering the knee meniscus.
abstract: The application of cell-based therapies in regenerative medicine is hindered by the difficulty of acquiring adequate numbers of competent cells. For the knee meniscus in particular, this may be solved by harvesting tissue from neighboring tendons and ligaments. In this study, we have investigated the potential of cells from tendon and ligament, as compared to meniscus cells, to engineer scaffold-free self-assembling fibrocartilage.
  
  
  
  Self-assembling meniscus-shaped constructs engineered from a co-culture of articular chondrocytes and either meniscus, tendon, or ligament cells were cultured for 4 weeks with TGF-β1 in serum-free media. After culture, constructs were assessed for their mechanical properties, histological staining, gross appearance, and biochemical composition including cross-link content. Correlations were performed to evaluate relationships between biochemical content and mechanical properties.
  
  
  
  In terms of mechanical properties as well as biochemical content, constructs engineered using tenocytes and ligament fibrocytes were found to be equivalent or superior to constructs engineered using meniscus cells. Furthermore, cross-link content was found to be correlated with engineered tissue tensile properties.
  
  
  
  Tenocytes and ligament fibrocytes represent viable cell sources for engineering meniscus fibrocartilage using the self-assembling process. Due to greater cross-link content, fibrocartilage engineered with tenocytes and ligament fibrocytes may maintain greater tensile properties than fibrocartilage engineered with meniscus cells.
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: P
authors.initials: P
authors.lastname: Hadidi
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: N K
authors.initials: NK
authors.lastname: Paschos
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Huang
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: A
authors.initials: A
authors.lastname: Aryaei
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Hu
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA; Department of Orthopedic Surgery, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA. Electronic address: athanasiou@ucdavis.edu.
authors.forename: K A
authors.initials: KA
authors.lastname: Athanasiou
mesh_terms: D002478:Cells, Cultured; D019902:Chondrocytes; D006801:Humans; D008022:Ligaments; D000072600:Meniscus; D013710:Tendons; D023822:Tissue Engineering
keywords: Knee surgery; Meniscus fibrocartilage; Primary cell sources; Self-assembling process; Tissue engineering