title: Hyperargininemia and renal oxidative stress: Prevention by antioxidants and NG-nitro-l-arginine methyl ester.
abstract: We investigated the in vitro and in vivo effects of arginine (Arg) on thiobarbituric acid-reactive substances (TBA-RS) and on the activities of catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) in renal tissues of rats. We also studied the influence of antioxidants (α-tocopherol plus ascorbic acid) and nitric oxide synthase inhibitor NG-nitro-l-arginine methyl ester (l-NAME) on the effects elicited by Arg. Results showed that Arg in vitro (1.5 mM) decreased SOD activity and increased the levels of TBA-RS in the renal medulla. Acute administration of Arg [0.8 g/kg, intraperitoneal injection] decreased CAT activity, increased SOD activity and TBA-RS levels in the renal medulla, and decreased CAT activity in the renal cortex of rats. Most results were prevented by antioxidants and/or l-NAME. Data indicate that Arg causes an oxidative imbalance in the renal tissues studied; however, in the presence of antioxidants and l-NAME, some of these alterations in oxidative stress were prevented.
authors.affiliation: Departamento de Medicina, Universidade da Região de Joinville- UNIVILLE, Rua Paulo Malschitzki, 10 - Zona Industrial Norte, CEP, 89201-972, Joinville, SC, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Delwing-de Lima
authors.affiliation: Departamento de Ciências Naturais, Centro de Ciências Exatas e Naturais, Universidade Regional de Blumenau, Rua Antônio da Veiga, 140, CEP 89012-900, Blumenau, SC, Brazil.
authors.forename: Débora
authors.initials: D
authors.lastname: Delwing-Dal Magro
authors.affiliation: Departamento de Farmácia, Universidade da Região de Joinville- UNIVILLE, Rua Paulo Malschitzki, 10 - Zona Industrial Norte, CEP, 89201-972, Joinville, SC, Brazil.
authors.forename: Cindy Laís Pett
authors.initials: CL
authors.lastname: Vieira
authors.affiliation: Departamento de Farmácia, Universidade da Região de Joinville- UNIVILLE, Rua Paulo Malschitzki, 10 - Zona Industrial Norte, CEP, 89201-972, Joinville, SC, Brazil.
authors.forename: Gislaine Maria Marestoni
authors.initials: GM
authors.lastname: Grola
authors.affiliation: Departamento de Farmácia, Universidade da Região de Joinville- UNIVILLE, Rua Paulo Malschitzki, 10 - Zona Industrial Norte, CEP, 89201-972, Joinville, SC, Brazil.
authors.forename: Débora Adriana
authors.initials: DA
authors.lastname: Fischer
authors.affiliation: Laboratório deNeuroproteção e Doenças Neurometabólicas, Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, Porto Alegre, RS, Brazil.
authors.forename: Angela Terezinha
authors.initials: AT
authors.lastname: de Souza Wyse
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D020162:Hyperargininemia / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D024502:alpha-Tocopherol; D019331:NG-Nitroarginine Methyl Ester
keywords: Arginine; antioxidant enzymes; antioxidants; oxidative stress; renal tissues