title: Magnetic Solid Phase Extraction Based on Oleic Acid Coated Fe3O4 for the Determination of Benzo[α]pyrene in Cigarette Smoke Coupled with High-Performance Liquid Chromatography.
abstract: In this work, a simple and effective method based on magnetic solid-phase extraction combined with high-performance liquid chromatography was developed for the determination of benzo[α]pyrene (BaP) in cigarette smoke. Oleic acid coated Fe3O4 (Fe3O4-OA) was synthesized and directly used as an efficient sorbent for the first time in magnetic solid-phase extraction (MSPE) procedure for the clean-up of BaP in cigarette smoke extracts. The synthesized Fe3O4-OA was characterized by transmission electron microscopy, X-ray diffraction and Fourier transformed infrared spectroscopy. The extraction via Fe3O4-OA was dispersed in the extracts of cigarette smoke followed by the magnetic isolation, acetonitrile-tetrahydrofuran (ACN-THF; v/v = 9:1) was used for desorption of the analyte. The effects of important parameters such as the amount of adsorbent, solution pH, the content of acetonitrile, temperature and sorption time were investigated. The method showed good linearity for the determination of BaP in the concentration range of 0.5-50 ng mL-1 with a regression coefficient (R2) of 0.9987. The limit of detection and limit of quantification for BaP were obtained to be 0.12 and 0.41 ng mL-1, respectively. The mean recoveries were in the range from 81.0% to 97.6% at low, medium, high spiked levels, and the relative standard deviations were in the range of 2.7-6.8%. Combined with high-performance liquid chromatography and fluorescence detection, a simple and effective method was developed for the analysis of BaP in cigarette smoke.
authors.affiliation: Technology Centre of China Tobacco Guangxi Industrial Co., LTD, Guangxi Province530001, China.
authors.forename: Shouai
authors.initials: S
authors.lastname: Feng
authors.affiliation: Technology Centre of China Tobacco Guangxi Industrial Co., LTD, Guangxi Province530001, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Wei
authors.affiliation: Technology Centre of China Tobacco Guangxi Industrial Co., LTD, Guangxi Province530001, China.
authors.forename: Zhaofu
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Technology Centre of China Tobacco Guangxi Industrial Co., LTD, Guangxi Province530001, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Li
authors.affiliation: Technology Centre of China Tobacco Guangxi Industrial Co., LTD, Guangxi Province530001, China.
authors.forename: Dongling
authors.initials: D
authors.lastname: Meng
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province650500, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Liao
authors.affiliation: Technology Center of Yunnan Tobacco Industry Limited Liability Company, Yunnan Province650231, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Miao
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province650500, China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Yang