title: Screening for Drugs Against the Plasmodium falciparum Digestive Vacuole by Imaging Flow Cytometry.
abstract: Phenotypic assays are increasingly employed to provide clues about drug mechanisms. In antimalarial drug screening, however, the majority of assays are designed to only measure parasite-killing activity. We describe here a high-content assay to detect drug-mediated perturbation of the digestive vacuole integrity in the trophozoite stage of Plasmodium falciparum, using the ImageStream imaging flow cytometer.
authors.affiliation: Department of Microbiology, National University of Singapore, Blk MD4, Level 3, 5 Science Drive 2, Singapore, 117545, Singapore.|NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore.
authors.forename: Yan Quan
authors.initials: YQ
authors.lastname: Lee
authors.affiliation: EMD Millipore, 645 Elliott Ave West, Suite 100, Seattle, WA, 98119, USA.
authors.forename: Brian E
authors.initials: BE
authors.lastname: Hall
authors.affiliation: Department of Microbiology, National University of Singapore, Blk MD4, Level 3, 5 Science Drive 2, Singapore, 117545, Singapore. kevin_tan@nuhs.edu.sg.|NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore. kevin_tan@nuhs.edu.sg.
authors.forename: Kevin S W
authors.initials: KS
authors.lastname: Tan
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D016778:Malaria, Falciparum / Q000188:drug therapy / Q000469:parasitology / Q000473:pathology; D010963:Plasmodium falciparum / Q000166:cytology* / Q000187:drug effects* / Q000254:growth & development; D014617:Vacuoles / Q000187:drug effects*
keywords: Apoptosis; Digestive vacuole; Drug discovery; Fluo-4; Imaging flow cytometry; Malaria; Plasmodium falciparum; Programmed cell death