title: Combined use of ionic liquid and sulfobutylether-β-cyclodextrin as electrolyte additives for separation and determination of camptothecin alkaloids by CZE.
abstract: This work reported that ionic liquid (IL) ([Bmim] [PF6]) and sulfobutylether-β-CD (SBE-β-CD) were used as electrolyte additives for the separation and determination of camptothecin (CPT) alkaloids by CZE. Separation parameters such as the buffer type, pH, and concentration of the running buffer, the concentration of SBE-β-CD and IL, temperature, and separation voltage were all investigated in order to achieve the maximum possible resolution. The four analytes were baseline separated within 10 min in capillary at the separation voltage of 15 kV with a running buffer consisting of 20 mM borate buffer, 20 mM IL, and 100 mM SBE-β-CD at pH 9.0. Under such conditions, good linearity about two orders of magnitudes of peak areas was achieved for the investigated CPT alkaloids with the correlation coefficients ranging from 0.9946 to 0.9985. For all analytes, detection limits (S/N = 3) and quantitation limits (S/N = 10) range from 0.05 to 0.92 μg/mL and 0.17 to 3.06 μg/mL, respectively. The proposed method has not only been successfully applied to the separation and determination of CPT alkaloids but also showed that IL seemed to be a promising additive in CZE separation.
authors.affiliation: College of Pharmacy, Shanxi Medical University, Taiyuan, China.
authors.forename: Yayun
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Translatinal Medicine Research Center, Shanxi Medical University, Taiyuan, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Yu
authors.affiliation: College of Pharmacy, Shanxi Medical University, Taiyuan, China.
authors.forename: Lou
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Pharmacy, Shanxi Medical University, Taiyuan, China.
authors.forename: Chenwu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Shanxi Medical University, Taiyuan, China.
authors.forename: Hongfen
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Shanxi Medical University, Taiyuan, China.
authors.forename: Guangbin
authors.initials: G
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Shanxi Medical University, Taiyuan, China.
authors.forename: Anjia
authors.initials: A
authors.lastname: Chen
mesh_terms: D002166:Camptothecin / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D007093:Imidazoles; D052578:Ionic Liquids; D047392:beta-Cyclodextrins; C093196:SBE4-beta-cyclodextrin; D002166:Camptothecin
keywords: CE; Camptothecin alkaloids; Ionic liquid; SBE-β-CD