title: Apparent seroprevalence, isolation and identification of risk factors for brucellosis among dairy cattle in Goa, India.
abstract: Brucellosis is a highly contagious zoonotic infection affecting livestock and human beings. The disease has been reported worldwide except in few countries where it has been eradicated. The prevalence of brucellosis among cattle from 11 farms having a history of abortions was studied. A total of 481 samples comprising of blood, milk, vaginal swabs, vaginal discharges, placental tissues and fetal tissues were collected from 296 animals. Clinical samples were processed for the isolation of Brucella. Serum samples (n=296) were tested by Rose Bengal Plate Test (RBPT) and indirect ELISA. A total of 90 (30.40%) and 123 (41.55%) samples were positive by RBPT and indirect ELISA, respectively. Also 27.02% samples were positive by both the tests. Brucella isolates (n= 8) were recovered from clinical samples using Brucella selective media. All the isolates demonstrated PCR amplification for the bcsp31 and IS711 genes. Amplification of Brucella abortus specific primer was demonstrated by all the isolates in AMOS PCR indicating isolates to be of either B. abortus biotype 1, 2 or 4. Risk factors for transmission of brucellosis among cattle population were studied by field surveys. It was observed that lack of awareness about brucellosis (OR=8.739, P=0.138) and inadequate floor space (OR=0.278, P=0.128) were crucial risk factors for transmission of bovine brucellosis.
authors.affiliation: Animal Sciences Section, ICAR Research Complex for Goa, Old Goa 403 402, India.
authors.forename: Ajay D
authors.initials: AD
authors.lastname: Pathak
authors.affiliation: Animal Sciences Section, ICAR Research Complex for Goa, Old Goa 403 402, India.
authors.forename: Z B
authors.initials: ZB
authors.lastname: Dubal
authors.affiliation: Animal Sciences Section, ICAR Research Complex for Goa, Old Goa 403 402, India.
authors.forename: M
authors.initials: M
authors.lastname: Karunakaran
authors.affiliation: Animal Sciences Section, ICAR Research Complex for Goa, Old Goa 403 402, India.
authors.forename: Swapnil P
authors.initials: SP
authors.lastname: Doijad
authors.affiliation: Animal Sciences Section, ICAR Research Complex for Goa, Old Goa 403 402, India.
authors.forename: Abhay V
authors.initials: AV
authors.lastname: Raorane
authors.affiliation: Goa State Co-Operative Milk Producers' Union Limited, Curti, Ponda 403 401, India.
authors.forename: R B
authors.initials: RB
authors.lastname: Dhuri
authors.affiliation: Goa State Co-Operative Milk Producers' Union Limited, Curti, Ponda 403 401, India.
authors.forename: M A
authors.initials: MA
authors.lastname: Bale
authors.affiliation: Animal Sciences Section, ICAR Research Complex for Goa, Old Goa 403 402, India.
authors.forename: Eaknath B
authors.initials: EB
authors.lastname: Chakurkar
authors.affiliation: Network Project on Brucellosis, Department of Microbiology and Animal Biotechnology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India.
authors.forename: Dewanand R
authors.initials: DR
authors.lastname: Kalorey
authors.affiliation: Network Project on Brucellosis, Department of Microbiology and Animal Biotechnology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India.
authors.forename: Nitin V
authors.initials: NV
authors.lastname: Kurkure
authors.affiliation: Animal Sciences Section, ICAR Research Complex for Goa, Old Goa 403 402, India. Electronic address: barbuddhesb@yahoo.com.
authors.forename: Sukhadeo B
authors.initials: SB
authors.lastname: Barbuddhe
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000235:genetics; D002003:Brucella abortus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D002007:Brucellosis, Bovine / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission; D002417:Cattle; D003612:Dairying; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D008892:Milk / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012307:Risk Factors; D016036:Seroepidemiologic Studies
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins
keywords: AMOS PCR; Brucella; Risk factors; Seroprevalence; Strain identification