title: Cyclophilin and enolase are the most prevalent conidial antigens of Lomentospora prolificans recognized by healthy human salivary IgA and cross-react with Aspergillus fumigatus.
abstract: The study of the immunocompetent airways immune response may provide important information to improve the therapeutic efficacy against Lomentospora (Scedosporium) prolificans. So, this study aimed to identify the most prevalent conidial antigens of this multiresistant fungus recognized by healthy human salivary immunoglobulin A, and to study their expression and cross-reactivity with other fungal species.
  
  
  
  Twenty saliva from immunocompetent donors were used to detect and identify the immunoreactive proteins by 2D immunoblotting and LC-MS/MS. Moreover, anti-Aspergillus antibodies were purified to study their cross-reactivity.
  
  
  
  Ten proteins of L. prolificans conidia showed reactivity with more than 50% of the saliva samples. Among them, cyclophilin and enolase were the most prevalent antigens recognized by 85 and 80% of the samples, respectively. These enzymes were also identified on the cell wall surface of L. prolificans and on the immunomes of Scedosporium apiospermum and Scedosporium aurantiacum. Additionally, they showed cross-reactivity with the most common pathogenic filamentous fungus Aspergillus fumigatus.
  
  
  
  These results show that the immunocompetent immune response might offer a pan-fungal recognition of conserved antigens such as enolase and cyclophilins, making them potential candidates for study as therapeutic targets.
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, Fungal and Bacterial Biomics Research Group, University of the Basque Country (UPV/EHU), Leioa, Spain.
authors.forename: Idoia
authors.initials: I
authors.lastname: Buldain
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, Fungal and Bacterial Biomics Research Group, University of the Basque Country (UPV/EHU), Leioa, Spain. andoni.ramirez@ehu.eus.
authors.forename: Andoni
authors.initials: A
authors.lastname: Ramirez-Garcia
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, Fungal and Bacterial Biomics Research Group, University of the Basque Country (UPV/EHU), Leioa, Spain.
authors.forename: Aize
authors.initials: A
authors.lastname: Pellon
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, Fungal and Bacterial Biomics Research Group, University of the Basque Country (UPV/EHU), Leioa, Spain.
authors.forename: Aitziber
authors.initials: A
authors.lastname: Antoran
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, Fungal and Bacterial Biomics Research Group, University of the Basque Country (UPV/EHU), Leioa, Spain.
authors.forename: Maria Jesus
authors.initials: MJ
authors.lastname: Sevilla
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, Fungal and Bacterial Biomics Research Group, University of the Basque Country (UPV/EHU), Leioa, Spain.
authors.forename: Aitor
authors.initials: A
authors.lastname: Rementeria
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, Fungal and Bacterial Biomics Research Group, University of the Basque Country (UPV/EHU), Leioa, Spain.
authors.forename: Fernando L
authors.initials: FL
authors.lastname: Hernando
mesh_terms: D000946:Antigens, Fungal / Q000276:immunology / Q000378:metabolism; D001232:Aspergillus fumigatus / Q000276:immunology*; D003429:Cross Reactions; D021983:Cyclophilins / Q000276:immunology* / Q000378:metabolism; D064368:Healthy Volunteers; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D010751:Phosphopyruvate Hydratase / Q000276:immunology* / Q000378:metabolism; D021381:Protein Transport; D012463:Saliva / Q000276:immunology*; D021681:Scedosporium / Q000276:immunology* / Q000378:metabolism; D013172:Spores, Fungal / Q000276:immunology
chemical_list: D000946:Antigens, Fungal; D007070:Immunoglobulin A; D010751:Phosphopyruvate Hydratase; D021983:Cyclophilins
keywords: 2DE; Aspergillus; Cross-reactivity; Immunome; Scedosporium