title: Validation of the HIV Tropism Test TROCAI Using the Virological Response to a Short-Term Maraviroc Monotherapy Exposure.
abstract: TROCAI is a phenotypic tropism test developed using the virological response to a short-term exposure to maraviroc monotherapy (Maraviroc Clinical Test [MCT]). It was found that with TROCAI, a cutoff of <0.5% of dual/mixed viruses was needed to predict R5 HIV tropism. Here, we have validated TROCAI, using this cutoff, in a new cohort of 42 patients, finding a very high concordance between TROCAI and MCT (98%), and a good concordance (71 to 87%) with other genotypic/phenotypic methods.
authors.affiliation: Molecular Immunobiology Laboratory, General Universitary Hospital Gregorio Marañon, Health Research Institute Gregorio Marañon, Spanish HIV HGM BioBank, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, IBIS/CSIC/SAS/University of Seville, Seville, Spain alextantalo@hotmail.com ezequiel.ruizmateos@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Gonzalez-Serna
authors.affiliation: Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, IBIS/CSIC/SAS/University of Seville, Seville, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Genebat
authors.affiliation: Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, IBIS/CSIC/SAS/University of Seville, Seville, Spain.
authors.forename: M
authors.initials: M
authors.lastname: De Luna-Romero
authors.affiliation: Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, IBIS/CSIC/SAS/University of Seville, Seville, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Tarancon-Diez
authors.affiliation: Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, IBIS/CSIC/SAS/University of Seville, Seville, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Dominguez-Molina
authors.affiliation: Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, IBIS/CSIC/SAS/University of Seville, Seville, Spain.
authors.forename: Y M
authors.initials: YM
authors.lastname: Pacheco
authors.affiliation: Molecular Immunobiology Laboratory, General Universitary Hospital Gregorio Marañon, Health Research Institute Gregorio Marañon, Spanish HIV HGM BioBank, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain Viral and Immune Infection Unit Center, Institute of Health Carlos III, Majadahonda Campus, Molecular Immunobiology Laboratory, General Universitary Hospital Gregorio Marañon, Madrid, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Muñoz-Fernández
authors.affiliation: Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, IBIS/CSIC/SAS/University of Seville, Seville, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Leal
authors.affiliation: Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, IBIS/CSIC/SAS/University of Seville, Seville, Spain alextantalo@hotmail.com ezequiel.ruizmateos@gmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Ruiz-Mateos
mesh_terms: D003510:Cyclohexanes / Q000494:pharmacology*; D006678:HIV / Q000187:drug effects* / Q000502:physiology; D023581:HIV Fusion Inhibitors / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D000077592:Maraviroc; D014230:Triazoles / Q000494:pharmacology*; D056189:Viral Tropism / Q000187:drug effects* / Q000502:physiology; D014773:Virology / Q000379:methods*
chemical_list: D003510:Cyclohexanes; D023581:HIV Fusion Inhibitors; D014230:Triazoles; D000077592:Maraviroc