title: Turkish propolis supresses MCF-7 cell death induced by homocysteine.
abstract: Elevated plasma homocysteine (Hcy) level is a most important risk factor for various vascular diseases including coronary, cerebral and peripheral arterial and venous thrombosis. Propolis is produced by honeybee from various oils, pollens and wax materials. Therefore, it has various biological properties including antioxidant, antitumor and antimicrobial activities. This study investigated the effects of propolis and Hcy on apoptosis in cancer cells. According to our findings, Hcy induced apoptosis in human breast adenocarcinoma (MCF-7) cells by regulating numerous genes and proteins involved in the apoptotic signal transduction pathway. In contrast, treatment with propolis inhibited caspase- 3 and -9 induced by Hcy in MCF-7 cells. It can be concluded that Hcy may augment the activity of anticancer agents that induce excessive reactive oxygen species (ROS) generation and apoptosis in their target cells. In contrast to the previous studies herein we found that propolis in low doses protected cancer cells inhibiting cellular apoptosis mediated by intracellular ROS-dependent mitochondrial pathway.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Bingol University, 12000 Bingol, Turkey. Electronic address: musatartik@hotmail.com.
authors.forename: Musa
authors.initials: M
authors.lastname: Tartik
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Bingol University, 12000 Bingol, Turkey. Electronic address: edarendelioglu@bingol.edu.tr.
authors.forename: Ekrem
authors.initials: E
authors.lastname: Darendelioglu
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Bingol University, 12000 Bingol, Turkey. Electronic address: gaykutoglu@bingol.edu.tr.
authors.forename: Gurkan
authors.initials: G
authors.lastname: Aykutoglu
authors.affiliation: Council of Higher Education, 06539, Bilkent, Ankara, Turkey. Electronic address: baydas@hotmail.com.
authors.forename: Giyasettin
authors.initials: G
authors.lastname: Baydas
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006710:Homocysteine / Q000633:toxicity*; D006801:Humans; D008054:Lipid Peroxides / Q000378:metabolism; D061986:MCF-7 Cells; D010636:Phenols / Q000032:analysis; D011429:Propolis / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014421:Turkey; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D008054:Lipid Peroxides; D010636:Phenols; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D006710:Homocysteine; D011429:Propolis
keywords: Homocysteine; LPO; MCF-7; Propolis; ROS; cDNA