title: Differential abundance of muscle proteome in cultured channel catfish (Ictalurus punctatus) subjected to ante-mortem stressors and its impact on fillet quality.
abstract: The effects of environmental and handling stress during catfish (Ictalurus punctatus) aquaculture were evaluated to identify the biochemical alterations they induce in the muscle proteome and their impacts on fillet quality. Temperature (25°C and 33°C) and oxygen (~2.5mg/L [L] and >5mg/L [H]) were manipulated followed by sequential socking (S) and transport (T) stress to evaluate changes in quality when fish were subjected to handling (25-H-ST; temperature-oxygen-handling), oxygen stress (25-L-ST), temperature stress (33-H-ST) and severe stress (33-L-ST). Instrumental color and texture of fillets were evaluated, and muscle proteome profile was analyzed. Fillet redness, yellowness and chroma decreased, and hue angle increased in all treatments except temperature stress (33-H-ST). Alterations in texture compared to controls were observed when oxygen levels were held high. In general, changes in the abundance of structural proteins and those involved in protein regulation and energy metabolism were identified. Rearing under hypoxic conditions demonstrated a shift in metabolism to ketogenic pathways and a suppression of the stress-induced changes as the severity of the stress increased. Increased proteolytic activity observed through the down-regulation of various structural proteins could be responsible for the alterations in color and texture.
authors.affiliation: Mississippi State University, Department of Food Science, Nutrition and Health Promotion, Herzer Building, 945 Stone Blvd, Box 9805, Mississippi State, MS 39762, United States; Mississippi State University, Department Wildlife, Fisheries and Aquaculture, Box 9690, Mississippi State, MS 39762, United States. Electronic address: mc2544@cornell.edu.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Ciaramella
authors.affiliation: University of Kentucky, Department of Animal and Food Sciences, Lexington, KY 40546, United States.
authors.forename: Mahesh N
authors.initials: MN
authors.lastname: Nair
authors.affiliation: University of Kentucky, Department of Animal and Food Sciences, Lexington, KY 40546, United States. Electronic address: spsuma2@uky.edu.
authors.forename: Surendranath P
authors.initials: SP
authors.lastname: Suman
authors.affiliation: Mississippi State University, Department Wildlife, Fisheries and Aquaculture, Box 9690, Mississippi State, MS 39762, United States. Electronic address: peter.allen@msstate.edu.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Allen
authors.affiliation: Mississippi State University, Department of Food Science, Nutrition and Health Promotion, Herzer Building, 945 Stone Blvd, Box 9805, Mississippi State, MS 39762, United States. Electronic address: schilling@foodscience.msstate.edu.
authors.forename: M Wes
authors.initials: MW
authors.lastname: Schilling
mesh_terms: D000818:Animals; D017756:Aquaculture; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004777:Environment; D005511:Food Handling; D007059:Ictaluridae / Q000254:growth & development / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D011786:Quality Control; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013312:Stress, Physiological
chemical_list: D009124:Muscle Proteins; D020543:Proteome
keywords: Color; Environmental stress; Handling stress; Proteomics; Texture