title: Genome editing comes of age.
abstract: Genome editing harnesses programmable nucleases to cut and paste genetic information in a targeted manner in living cells and organisms. Here, I review the development of programmable nucleases, including zinc finger nucleases (ZFNs), TAL (transcription-activator-like) effector nucleases (TALENs) and CRISPR (cluster of regularly interspaced palindromic repeats)-Cas9 (CRISPR-associated protein 9) RNA-guided endonucleases (RGENs). I specifically highlight the key advances that set the foundation for the rapid and widespread implementation of CRISPR-Cas9 genome editing approaches that has revolutionized the field.
authors.affiliation: Center for Genome Engineering, Institute for Basic Science, Seoul, Republic of Korea.|Department of Chemistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems / Q000235:genetics; D002875:Chromosomes / Q000235:genetics; D003851:Deoxyribonucleases / Q000378:metabolism; D000072669:Gene Editing / Q000379:methods*; D015316:Genetic Therapy; D016678:Genome / Q000235:genetics*; D006801:Humans
chemical_list: D003851:Deoxyribonucleases