title: Premature mammary gland involution with repeated corticosterone injection in interleukin 10-deficient mice.
abstract: Recently, we found that maternal stress could induce premature mammary gland involution in interleukin 10 knock out (IL-10-/-) mice. To elucidate correlation between stress, IL-10, and mammary gland involution, corticosterone was injected into the lactating wild type and IL-10-deficient mice and assessed mammary gland phenotype. Repetitive corticosterone injection developed premature mammary gland involution only in B6.IL-10-/-mice; moreover, it induced alopecia in nursing pups. Corticosterone injection induced several typical changes such as mammary gland epithelial cell apoptosis, macrophage infiltration, fat deposition in adipocyte, STAT3 phosphorylation, and upregulation of tyrosine hydroxylase gene in adrenal gland. Overall incidence of pup alopecia and mammary gland involution was relatively high in corticosterone than control B6.IL-10-/-group (57% vs. 20%). Our finding demonstrates that IL-10 is important for stress modulation, and B6.Il-10-/-with corticosterone has several advantage such as simple to establish, well-defined onset of mammary gland involution, high incidence, and inducing pup alopecia.
authors.affiliation: a Designed Animal and Transplantation Research Institute , Institute of Greenbio Science and Technology, Seoul National University , Pyeongchang , Korea.
authors.forename: Woo-Sung
authors.initials: WS
authors.lastname: Hwang
authors.affiliation: a Designed Animal and Transplantation Research Institute , Institute of Greenbio Science and Technology, Seoul National University , Pyeongchang , Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Bae
authors.affiliation: b Graduate School of International Agricultural Technology , Seoul National University , Pyeongchang , Korea.
authors.forename: Su-Cheong
authors.initials: SC
authors.lastname: Yeom
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D000505:Alopecia / Q000209:etiology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003345:Corticosterone / Q000494:pharmacology*; D005260:Female; D016753:Interleukin-10 / Q000172:deficiency*; D007774:Lactation / Q000187:drug effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D016753:Interleukin-10; D003345:Corticosterone
keywords: interleukin 10; mammary gland involution; stress