title: Effect of 3-keto-1,5-bisphosphonates on obese-liver's rats.
abstract: Obesity is associated with an oxidative stress status, which is defined by an excess of reactive oxygen species (ROS) vs. the antioxidant defense system. We report in this present work, the link between fat deposition and oxidative stress markers using a High Fat Diet-(HFD) induced rat obesity and liver-oxidative stress. We further determined the impact of chronic administration of 3-keto-1, 5-BPs 1 (a & b) (40μg/kg/8 weeks/i.p.) on liver's level. In fact, exposure of rats to HFD during 16 weeks induced body and liver weight gain and metabolic disruption with an increase on liver Alanine amino transférase (ALAT) and Aspartate aminotransférase (ASAT) concentration. HFD increased liver calcium level as well as free iron, whereas, it provoked a decrease on liver lipase activity. HFD also induced liver-oxidative stress status vocalized by an increase in reactive oxygen species (ROS) as superoxide radical (O2), hydroxyl radical (OH) and Hydrogen peroxide (H2O2). Consequently, different deleterious damages as an increase on Malon Dialdehyde MDA, Carbonyl protein PC levels with a decrease in non-protein sulfhydryls NPSH concentrations, have been detected. Interestingly, our results demonstrate a decrease in antioxidant enzymes activities such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx) and peroxidases (POD). Importantly, 3-keto-1,5-bisphosphonates treatment corrected the majority of the deleterious effects caused by HFD, but it failed to correct some liver's disruptions as mineral profile, oxidative damages (PC and NPSH levels) as well as SOD and lipase activities. Our investigation point that 3-keto-1,5-bisphosphonates could be considered as safe antioxidant agents on the hepatic level that should also find other potential biological applications.
authors.affiliation: Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerta, University of Carthage, 7021, Jarzouna, Tunisia. Electronic address: lakarima29@gmail.com.
authors.forename: Karima
authors.initials: K
authors.lastname: Lahbib
authors.affiliation: Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerta, University of Carthage, 7021, Jarzouna, Tunisia.
authors.forename: Soufiane
authors.initials: S
authors.lastname: Touil
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004164:Diphosphonates / Q000737:chemistry / Q000494:pharmacology*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008297:Male; D009765:Obesity / Q000097:blood / Q000473:pathology*; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004164:Diphosphonates; D008055:Lipids; D017382:Reactive Oxygen Species
keywords: 3-Keto-1,5-bisphosphonates; High fat diet; Liver lipase activity; Liver-oxidative stress; Metabolic disruption; Mineral profile; Obesity