title: Advanced oxidation protein products induce catabolic effect through oxidant-dependent activation of NF-κ B pathway in human chondrocyte.
abstract: Advanced oxidation protein products (AOPPs) have been shown to participate in the progression of rheumatoid arthritis (RA). However, the effect of AOPPs accumulation on catabolic effect in human chondrocyte and the underlying mechanism(s) remain unclear. The present study demonstrated that AOPPs inhibited cell viability and glycosaminoglycan (GAG) production in human chondrocyte. Exposure of chondrocyte to AOPPs significantly increased the production of catabolic factors, such as cyclooxygenase-2 (COX-2), matrix metalloproteinase 3 (MMPs)-3 and MMP-13. AOPPs stimulation induced ROS generation and NF-κ B p65 phosphorylation, which could be inhibited by soluble receptor for advanced glycan end products (sRAGE), NADPH oxidase inhibitor (apocynin), ROS scavenger (N-acetyl-cysteine, NAC). Furthermore, NF-κ B inhibitor Bay11-7082 significantly reversed the AOPPs-induced expression of catabolic factors and phosphorylation of NF-κ B p65. Targeting AOPPs-triggered catabolic effect might be as a promising option for patients with RA.
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Ye
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, China.
authors.forename: Zhaoming
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, China.
authors.forename: Siyuan
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Orthopedic Joint Surgery, Nanfang Hospital, Southern Medical University, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Pharmacy, Nanfang Hospital, Southern Medical University, China.
authors.forename: Shaolian
authors.initials: S
authors.lastname: Song
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, China. Electronic address: chenjianting99@126.com.
authors.forename: Jianting
authors.initials: J
authors.lastname: Chen
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000378:metabolism*; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D019902:Chondrocytes / Q000502:physiology*; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D052246:Cyclooxygenase 2 Inhibitors / Q000378:metabolism; D005260:Female; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D008875:Middle Aged; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology; D010084:Oxidation-Reduction; D015398:Signal Transduction / Q000187:drug effects; D013450:Sulfones / Q000494:pharmacology
chemical_list: C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; D062107:Advanced Oxidation Protein Products; D002373:Cyclic AMP Receptor Protein; D052246:Cyclooxygenase 2 Inhibitors; D017127:Glycation End Products, Advanced; D006025:Glycosaminoglycans; D016328:NF-kappa B; D009570:Nitriles; D013450:Sulfones; D053509:Matrix Metalloproteinase 13; D019278:Matrix Metalloproteinase 3
keywords: Advanced oxidation protein products (AOPPs); Catabolism; Chondrocytes; Oxidative; Rheumatoid arthritis; Stress