title: Protective Effect of Quercetin on Posttraumatic Cardiac Injury.
abstract: Quercetin is an important dietary flavonoid present in fruits and vegetables and has attracted attention because of its anti-inflammatory and anti-oxidative properties. Inflammation and oxidative stress play important roles in posttraumatic cardiomyocyte apoptosis, which contributes to secondary cardiac dysfunction. This study investigates the protective effect of quercetin on trauma-induced secondary cardiac injury and the mechanisms involved. Widely accepted nonlethal mechanical trauma models were established. In vivo, cardiomyocyte apoptosis and cardiac dysfunction in rats were assessed using TUNEL staining and a biological mechanic experiment system. In vitro, cell viability, tumour necrosis factor-α (TNF-α), reactive oxygen species (ROS) and [Ca(2+)]i of H9c2 cells were detected using an MTT assay, ELISA, and 2',7'-dichlorofluorescin diacetate and fluo-4 acetoxymethyl ester assays respectively. Quercetin pretreatment (20 mg/kg i.p.; 0.5 h before trauma) significantly improved posttraumatic cardiomyocyte apoptosis and cardiac dysfunction. Pretreatment with quercetin (20 μM; 24 h before trauma plasma addition) significantly attenuated trauma-induced viability decreases, TNF-α increases, ROS overproduction and [Ca(2+)]i overload in H9c2 cells. In conclusion, quercetin may reverse posttraumatic cardiac dysfunction by reducing cardiomyocyte apoptosis through the suppression of TNF-α increases, ROS overproduction and Ca(2+) overload in cardiomyocytes, representing a potential preventive approach for the treatment of secondary cardiac injury after mechanical trauma.
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Zehao
authors.initials: Z
authors.lastname: Jing
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Zhuorun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Xiujie
authors.initials: X
authors.lastname: Li
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Xintao
authors.initials: X
authors.lastname: Li
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Cao
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Deqin
authors.initials: D
authors.lastname: Yu
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Shuzhuang
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D006335:Heart Injuries / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects; D011794:Quercetin / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D011794:Quercetin; D002118:Calcium