title: Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression.
abstract: We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25≤BCS≤3.5) and high (4.0≤BCS≤4.25). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, β-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IRβ), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IRβ, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.
authors.affiliation: Department of Ruminants and Swine Diseases, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Radiša
authors.initials: R
authors.lastname: Prodanović
authors.affiliation: Laboratory for Molecular Biology and Endocrinology, Vinča Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Goran
authors.initials: G
authors.lastname: Korićanac
authors.affiliation: Department of Ruminants and Swine Diseases, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Vujanac
authors.affiliation: Department of Biochemistry, Institute for Biological Research "Siniša Stankovic", University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Ana
authors.initials: A
authors.lastname: Djordjević
authors.affiliation: Laboratory for Molecular Biology and Endocrinology, Vinča Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Marija
authors.initials: M
authors.lastname: Pantelić
authors.affiliation: Laboratory for Molecular Biology and Endocrinology, Vinča Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Snježana
authors.initials: S
authors.lastname: Romić
authors.affiliation: Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Zoran
authors.initials: Z
authors.lastname: Stanimirović
authors.affiliation: Department of Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia. Electronic address: dani@vet.bg.ac.rs.
authors.forename: Danijela
authors.initials: D
authors.lastname: Kirovski
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005234:Fatty Liver / Q000209:etiology / Q000378:metabolism / Q000662:veterinary*; D005260:Female; D005947:Glucose / Q000378:metabolism; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D007774:Lactation; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism / Q000662:veterinary*; D011247:Pregnancy; D051780:Sterol Regulatory Element Binding Protein 1 / Q000097:blood / Q000235:genetics / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D018955:CD36 Antigens; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D051780:Sterol Regulatory Element Binding Protein 1; D005947:Glucose; D020155:3-Hydroxybutyric Acid
keywords: CD36; Dairy cows; Fatty liver; Sterol regulatory element-binding protein-1