title: Comparison of in vitro digestion model with in vivo relative bioavailability of BDE-209 in indoor dust and combination of in vitro digestion/Caco-2 cell model to estimate the daily intake of BDE-209 via indoor dust.
abstract: There is limited information on the BDE-209 relative bioavailability (RBA) of indoor dust and the absorption of BDE-209 after in vitro digestion was seldom studied. In the present study, BDE-209 RBA in 6 household dust samples measured using an in vivo mouse model was compared to BDE-209 bioaccessibility determined using physiologically based extraction test (PBET) and solubility bioaccessibility research consortium method (SBRC) assays. BDE-209 RBA obtained ranged from 45.9 ± 16.1 to 96.0 ± 17.4% and exhibited a significant relationship with PBET gastric phase (r2= 0.578, p = 0.080), small intestinal phase (r2= 0.728, p = 0.031) and total BDE-209 bioaccessibility (r2= 0.728, p = 0.031), which indicated PBET assay can serve as a surrogate to predict BDE-209 RBA to refine human health exposure. In addition, the absorption of BDE-209 by Caco-2 cell line was assessed. With the consideration of the corresponding bioaccessibility and absorption of BDE-209 by Caco-2 cell line, the human daily intake of BDE-209 via dust ingestion for adults and children was much lower than that estimated by total concentration.
authors.affiliation: School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China; Guangdong Engineering Technology Research Center for Drinking Water Safety, School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China.
authors.forename: Weijian
authors.initials: W
authors.lastname: Pan
authors.affiliation: School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China; Guangdong Engineering Technology Research Center for Drinking Water Safety, School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China. Electronic address: kangyuan@m.scnu.edu.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Kang
authors.affiliation: School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China; Guangdong Engineering Technology Research Center for Drinking Water Safety, School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China.
authors.forename: Lixuan
authors.initials: L
authors.lastname: Zeng
authors.affiliation: School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China; Guangdong Engineering Technology Research Center for Drinking Water Safety, School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China.
authors.forename: Qiuyun
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China; Guangdong Engineering Technology Research Center for Drinking Water Safety, School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China.
authors.forename: Jiwen
authors.initials: J
authors.lastname: Luo
authors.affiliation: Consortium on Health, Environment, Education and Research (CHEER), and Department of Science and Environmental Studies, Hong Kong Institute of Education, Hong Kong, People's Republic of China; School of Environment, Jinan University, Guangzhou, People's Republic of China.
authors.forename: Ming Hung
authors.initials: MH
authors.lastname: Wong
mesh_terms: D000818:Animals; D001681:Biological Assay; D001682:Biological Availability; D018938:Caco-2 Cells; D004391:Dust / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis / Q000378:metabolism*; D005260:Female; D055768:Halogenated Diphenyl Ethers / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D051379:Mice; D012995:Solubility
chemical_list: D004391:Dust; D004785:Environmental Pollutants; D055768:Halogenated Diphenyl Ethers; C010902:decabromobiphenyl ether
keywords: BDE-209; Caco-2 cells; In vitro digestion; Relative bioavailability