title: Chicken parvovirus and its associations with malabsorption syndrome.
abstract: Malabsorption syndrome (MAS) is a multifactorial syndrome which is characterized by enteric disorders and reduced growth rates of broilers. Such condition is responsible for significant economic losses to the poultry industry. A possible association between chicken parvovirus (ChPV) infections and the occurrence of MAS has been proposed. However, such association has not to date been elucidated in view that ChPV has been detected in healthy as well as in MAS-affected chickens. This study aimed to detect and quantify ChPV loads in sera and tissues of MAS-affected, as well as in healthy broilers. Fifty nine, 39-day-old broilers (50 diseased, 9 healthy birds), obtained from the same flocks, were examined. The highest ChPV DNA loads were detected in MAS-affected broilers, particularly in fecal samples and intestinal tissues (~5500 genomic copies/300ng of total DNA). The average viral genome load in serum in MAS-affected birds was 1134copies/mL, whereas no viral DNA was found in sera and thymus tissues from healthy animals. These findings reveal that MAS-affected broilers consistently carry ChPV DNA is serum, whereas healthy animals do not. In addition, viral loads in tissues (bursa of Fabricius, spleen, intestine and liver) of MAS-affected birds were significantly higher in comparison to the same tissues from healthy broilers. Although preliminary, the results obtained here indicate an association between the detection of ChPV DNA in serum, in addition to high ChPV viral loads in tissues, and the occurrence of MAS in broilers. Further experiments should be performed to confirm such results.
authors.affiliation: FEPAGRO Saúde Animal - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, CEP 92990-000 Rio Grande, do Sul, Brazil; Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, sala 08, Porto Alegre, CEP 90050-170 Rio Grande, do Sul, Brazil. Electronic address: fabrinefinkler@gmail.com.
authors.forename: F
authors.initials: F
authors.lastname: Finkler
authors.affiliation: FEPAGRO Saúde Animal - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, CEP 92990-000 Rio Grande, do Sul, Brazil.
authors.forename: D A
authors.initials: DA
authors.lastname: Lima
authors.affiliation: FEPAGRO Saúde Animal - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, CEP 92990-000 Rio Grande, do Sul, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Cerva
authors.affiliation: FEPAGRO Saúde Animal - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, CEP 92990-000 Rio Grande, do Sul, Brazil.
authors.forename: L B
authors.initials: LB
authors.lastname: Moraes
authors.affiliation: FEPAGRO Saúde Animal - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, CEP 92990-000 Rio Grande, do Sul, Brazil; Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, sala 08, Porto Alegre, CEP 90050-170 Rio Grande, do Sul, Brazil.
authors.forename: S P
authors.initials: SP
authors.lastname: Cibulski
authors.affiliation: FEPAGRO Saúde Animal - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, CEP 92990-000 Rio Grande, do Sul, Brazil; Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, sala 08, Porto Alegre, CEP 90050-170 Rio Grande, do Sul, Brazil.
authors.forename: T F
authors.initials: TF
authors.lastname: Teixeira
authors.affiliation: FEPAGRO Saúde Animal - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, CEP 92990-000 Rio Grande, do Sul, Brazil; Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, sala 08, Porto Alegre, CEP 90050-170 Rio Grande, do Sul, Brazil.
authors.forename: H F
authors.initials: HF
authors.lastname: Santos
authors.affiliation: FEPAGRO Saúde Animal - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, CEP 92990-000 Rio Grande, do Sul, Brazil.
authors.forename: L L
authors.initials: LL
authors.lastname: Almeida
authors.affiliation: FEPAGRO Saúde Animal - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, CEP 92990-000 Rio Grande, do Sul, Brazil; Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, sala 08, Porto Alegre, CEP 90050-170 Rio Grande, do Sul, Brazil.
authors.forename: P M
authors.initials: PM
authors.lastname: Roehe
authors.affiliation: Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, sala 08, Porto Alegre, CEP 90050-170 Rio Grande, do Sul, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Franco
mesh_terms: D000818:Animals; D002060:Bursa of Fabricius; D002645:Chickens; D007422:Intestines / Q000821:virology; D008286:Malabsorption Syndromes / Q000662:veterinary* / Q000821:virology; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017992:Parvovirus / Q000235:genetics*; D011201:Poultry Diseases / Q000821:virology*
keywords: Aveparvovirus; Parvoviridae; Poultry; Quantitative PCR; Runting-stunting syndrome (RSS)