title: Nephroprotection of punicalagin in rat model of endotoxemic acute kidney injury.
abstract: The potential nephroprotection of punicalagin (PNG) against lipopolysaccharide (LPS)-induced acute kidney injury in rats was investigated. Rats received a single i.v. dose of LPS (5 mg/kg), and treated with PNG (50 mg/kg, i.p.), 1 h before, and 1 h following LPS administration. LPS caused significant increases of serum creatinine and neutrophil gelatinase-associated lipocalin. LPS also resulted in significant increases in interleukin-18, tumor necrosis factor-α, interleukin-6, malondialdehyde, nitric oxide, Bax/Bcl-2 ratio and myeloperoxidase, inducible nitric oxide synthase, caspases 3, 8 and 9 activities, and a significant decrease in total antioxidant capacity in kidney tissues. PNG significantly ameliorated the alterations in the measured parameters. Additionally, PNG attenuated the histopathological injury and reduced kidney injury molecule-1 expression in kidneys of rats that received LPS. It was concluded that PNG ameliorated endotoxemic acute kidney injury in rats by counteracting inflammation, oxidative/nitrative stress and apoptosis.
authors.affiliation: a Department of Biomedical Sciences, Pharmacology Division, College of Medicine , King Faisal University , Al-Ahsa , Saudi Arabia.
authors.forename: Amr A
authors.initials: AA
authors.lastname: Fouad
authors.affiliation: b Department of Internal Medicine, College of Medicine , King Faisal University , Al-Ahsa , Saudi Arabia.
authors.forename: Hatem O
authors.initials: HO
authors.lastname: Qutub
authors.affiliation: c Department of Biomedical Sciences, Histopathology Division, College of Medicine , King Faisal University , Al-Ahsa , Saudi Arabia.
authors.forename: Walid N
authors.initials: WN
authors.lastname: Al-Melhim
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D047348:Hydrolyzable Tannins / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007677:Kidney Function Tests; D008070:Lipopolysaccharides / Q000633:toxicity*; D029561:Lythraceae / Q000737:chemistry; D008297:Male; D010936:Plant Extracts / Q000737:chemistry; D020011:Protective Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley
chemical_list: D047348:Hydrolyzable Tannins; D008070:Lipopolysaccharides; D010936:Plant Extracts; D020011:Protective Agents; C440499:lipopolysaccharide, Escherichia coli O111 B4; C115642:punicalagin
keywords: Endotoxemia; kidney; punicalagin; rats