title: Synthetic receptor molecules for selective fluorescence detection of 8-oxo-dGTP in aqueous media.
abstract: A series of 9-hydroxy-1,3-diazaphenoxazine-2-one derivatives were synthesized as fluorescent receptor molecules for 8-oxo-dGTP, which attach the cyclen-zinc complex at the 3-N position as the binding site for the triphosphate and the (2-aryloxycarbonylamino)ethyl group at the 9-O position as the hydrogen bonding site for 8-oxoguanine. Among these molecules, the receptor molecule 5a-Zn constructed of the ethyl linker at 3-N and the (2-benzyloxycarbonyl amino)ethyl group at 9-O displayed the best recognition ability for 8-oxoguanosine triphosphate (8-oxo-dGTP) in aqueous media. The receptor 5a-Zn was also shown to selectively detect 8-oxo-dGTP in a cell lysate solution.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. sasaki@phar.kyushu-u.ac.jp.
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Fuchi
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. sasaki@phar.kyushu-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Fukuda
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. sasaki@phar.kyushu-u.ac.jp.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sasaki
mesh_terms: D001372:Aza Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D003848:Deoxyguanine Nucleotides / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D010078:Oxazines / Q000138:chemical synthesis / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D001372:Aza Compounds; D003848:Deoxyguanine Nucleotides; D005456:Fluorescent Dyes; D010078:Oxazines; D014867:Water; C078206:8-oxodeoxyguanosine triphosphate