title: Modulation of LINE-1 Retrotransposition by a Human SAMHD1 Polymorphism.
abstract: The HIV-1 restriction factor SAMHD1 has the ability to negatively modulate retrotransposition of the long interspersed element 1(LINE-1). By exploring the ability of human SAMHD1 polymorphisms to inhibit LINE-1, we found that the single nucleotide polymorphism S33A present in the Korean population lose the ability to inhibit LINE-1 retrotransposition. Because SAMHD1 residue S33 is phosphorylated in human cycling and non-cycling cells, we demonstrated that SAMHD1 requires to be either phosphorylated on position 33 or to contain a bulky residue in order to inhibit LINE-1 retrotransposition. Therefore this unique mutation uncouples functions in this important restriction factor.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine Bronx, NY 10461.
authors.forename: Tommy E
authors.initials: TE
authors.lastname: White
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine Bronx, NY 10461.
authors.forename: Alberto
authors.initials: A
authors.lastname: Brandariz-Nuñez
authors.affiliation: Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea.
authors.forename: Kyudong
authors.initials: K
authors.lastname: Han
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, 80309, USA.
authors.forename: Sara L
authors.initials: SL
authors.lastname: Sawyer
authors.affiliation: Department of Pediatrics, Emory University, Atlanta, GA 30322.
authors.forename: Baek
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine Bronx, NY 10461.
authors.forename: Felipe
authors.initials: F
authors.lastname: Diaz-Griffero
keywords: HIV-1; LINE-1; SAMHD1; SNPs; restrcition; retrotransposition