title: High-Throughput Baculovirus Expression System for Membrane Protein Production.
abstract: The ease of use, robustness, cost-effectiveness, and posttranslational machinery make baculovirus expression system a popular choice for production of eukaryotic membrane proteins. This system can be readily adapted for high-throughput operations. This chapter outlines the techniques and procedures for cloning, transfection, small-scale production, and purification of membrane protein samples in a high-throughput manner.
authors.affiliation: New York Structural Biology Center, New York Consortium on Membrane Protein Structure (NYCOMPS), 89 Convent Avenue, New York, NY, 10027, USA. ravik@nysbc.org.
authors.forename: Ravi C
authors.initials: RC
authors.lastname: Kalathur
authors.affiliation: New York Structural Biology Center, New York Consortium on Membrane Protein Structure (NYCOMPS), 89 Convent Avenue, New York, NY, 10027, USA.
authors.forename: Marinela
authors.initials: M
authors.lastname: Panganiban
authors.affiliation: New York Structural Biology Center, New York Consortium on Membrane Protein Structure (NYCOMPS), 89 Convent Avenue, New York, NY, 10027, USA.
authors.forename: Renato
authors.initials: R
authors.lastname: Bruni
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D015870:Gene Expression; D057166:High-Throughput Screening Assays; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D061987:Sf9 Cells; D014162:Transfection
chemical_list: D008565:Membrane Proteins
keywords: Bacmid; Baculovirus; Expression; High-throughput; Insect cell; Membrane protein; Nickel affinity chromatography; Purification; Sf9