title: Mitochondria-targeting ratiometric fluorescent probe for γ-glutamyltranspeptidase and its application to colon cancer.
abstract: A mitochondria-targeting ratiometric probe was designed for γ-glutamyltranspeptidase (γGT). Mechanistic study by HPLC and an inhibitor assay showed that the probe underwent γGT-mediated amide-to-amine transformation and induced a ratiometric fluorescence response in cellular mitochondria. Further application was successful for the detection of cancerous colons in mice.
authors.affiliation: Department of Chemistry, Hankuk University of Foreign Studies, Yongin 449-791, Korea. haejkim@hufs.ac.kr.
authors.forename: Seokan
authors.initials: S
authors.lastname: Park
authors.affiliation: ASAN Institute for Life Sciences, ASAN Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea.
authors.forename: Dong Jun
authors.initials: DJ
authors.lastname: Bae
authors.affiliation: ASAN Institute for Life Sciences, ASAN Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea.
authors.forename: Yeon-Mi
authors.initials: YM
authors.lastname: Ryu
authors.affiliation: ASAN Institute for Life Sciences, ASAN Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea and Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea.
authors.forename: Sang-Yeob
authors.initials: SY
authors.lastname: Kim
authors.affiliation: ASAN Institute for Life Sciences, ASAN Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea and Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea. sjmyung@amc.seoul.kr.
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Myung
authors.affiliation: Department of Chemistry, Hankuk University of Foreign Studies, Yongin 449-791, Korea. haejkim@hufs.ac.kr.
authors.forename: Hae-Jo
authors.initials: HJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D005973:Glutamine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D045325:HCT116 Cells; D006801:Humans; D006868:Hydrolysis; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D057230:Limit of Detection; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D005723:gamma-Glutamyltransferase / Q000032:analysis* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; D005973:Glutamine; D005723:gamma-Glutamyltransferase