title: Identification of Natural Infections in Sheep/Goats with HoBi-like Pestiviruses in China.
abstract: The natural infections of HoBi-like pestiviruses in cattle have been reported in South America, Europe and Asia. In China, although the detections of HoBi-like pestivirus have been reported, the epidemiological investigation was limited. From January 2014 to October 2015, several flocks of sheep/goats in Henan province in central China suffered respiratory diseases which were recovered slowly after antibiotics treatment. To test whether it is the HoBi-like pestivirus caused this symptom, 49 serum samples and 22 nasal swabs were then collected for analysis by serology and RT-PCR. Serological result revealed that prevalence of pestivirus in small ruminants was 12.2% (6/49) in central China. Sequence analysis of partial 5'-UTR nucleotides of pestivirus-positive samples suggested that HoBi-like pestivirus might have circulated in sheep/goats of China for a period and have evolved into new genotype clusters. It is apparent that the study provides the molecular evidence of natural infections in goat/sheep species with HoBi-like pestiviruses in China.
authors.affiliation: Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Libratory of Insect Biology, Nanyang Normal University, Nanyang, China.
authors.forename: H
authors.initials: H
authors.lastname: Shi
authors.affiliation: Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Libratory of Insect Biology, Nanyang Normal University, Nanyang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Kan
authors.affiliation: Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Libratory of Insect Biology, Nanyang Normal University, Nanyang, China.
authors.forename: L
authors.initials: L
authors.lastname: Yao
authors.affiliation: Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Libratory of Insect Biology, Nanyang Normal University, Nanyang, China.
authors.forename: C
authors.initials: C
authors.lastname: Leng
authors.affiliation: Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Libratory of Insect Biology, Nanyang Normal University, Nanyang, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Libratory of Insect Biology, Nanyang Normal University, Nanyang, China.
authors.forename: J
authors.initials: J
authors.lastname: Ji
authors.affiliation: Liao Ning Center for Animal Disease Control and Prevention, Shenyang, China.
authors.forename: S
authors.initials: S
authors.lastname: Sun
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D005838:Genotype; D006041:Goats / Q000821:virology; D010576:Pestivirus / Q000235:genetics*; D018182:Pestivirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D012756:Sheep / Q000821:virology
keywords: Bovine viral diarrhoea virus; China; HoBi-like pestiviruses; goat; sheep