title: Total Protein Profile and Drug Resistance in Candida albicans Isolated from Clinical Samples.
abstract: This study was done to assess the antifungal susceptibility of clinical isolates of Candida albicans and to evaluate its total protein profile based on morphological difference on drug resistance. Hundred and twenty clinical isolates of C. albicans from various clinical specimens were tested for susceptibility against four antifungal agents, namely, fluconazole, itraconazole, amphotericin B, and ketoconazole. A significant increase of drug resistance in clinical isolates of C. albicans was observed. The study showed 50% fluconazole and itraconazole resistance at 32 μg mL(-1) with a MIC50 and MIC90 values at 34 and 47 and 36 and 49 μg mL(-1), respectively. All isolates were sensitive to amphotericin B and ketoconazole. The SDS-PAGE protein profile showed a prevalent band of ~52.5 kDa, indicating overexpression of gene in 72% strains with fluconazole resistance. Since the opportunistic infections of Candida spp. are increasing along with drug resistance, the total protein profile will help in understanding the evolutionary changes in drug resistance and also to characterize them.
authors.affiliation: Biotechnology Pharmacology Laboratory, Centre for Scientific Research & Development, People's University, Bhopal 462037, India.
authors.forename: Kamal Uddin
authors.identifier: 0000-0002-0217-7534
authors.initials: KU
authors.lastname: Zaidi
authors.affiliation: Biotechnology Pharmacology Laboratory, Centre for Scientific Research & Development, People's University, Bhopal 462037, India.
authors.forename: Abin
authors.identifier: 0000-0001-6979-189X
authors.initials: A
authors.lastname: Mani
authors.affiliation: Biotechnology Pharmacology Laboratory, Centre for Scientific Research & Development, People's University, Bhopal 462037, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Thawani
authors.affiliation: Biotechnology Pharmacology Laboratory, Centre for Scientific Research & Development, People's University, Bhopal 462037, India.
authors.forename: Arti
authors.initials: A
authors.lastname: Mehra