title: A simultaneous diagnosis and genotyping method for global surveillance of cetacean morbillivirus.
abstract: Cetacean morbillivirus (CeMV) is considered one of the most important viral pathogens in cetaceans. CeMV outbreaks of lethal disease have repeatedly been observed in Europe, the Americas, and Australia, while large herds of gregarious species were found to be the likely reservoirs and sources of CeMV infection to susceptible species in the Atlantic and Pacific Oceans. Furthermore, three new strains were detected recently in Hawaii, Brazil and Australia. To clarify the real global distribution of CeMV and possible carriers, we showed a novel technique successfully diagnosing and distinguishing different virus strains (DMV, PWMV and novel CeMVs) using FFPE samples from 1996 to 2011. This efficient method that combines qRT-PCR and high resolution melting (HRM) could be applied to the future retrospective global studies for better understanding of different prevalence and outbreak conditions among ocean basins and the mechanism of variable host response to pathogens.
authors.affiliation: National Chiayi University, Chiayi, Taiwan.
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Yang
authors.affiliation: National Chiayi University, Chiayi, Taiwan.
authors.forename: Bi-Jhen
authors.initials: BJ
authors.lastname: Wu
authors.affiliation: University of Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, Canary Islands, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Sierra
authors.affiliation: University of Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, Canary Islands, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Fernandez
authors.affiliation: University of São Paulo, São Paulo, Brazil.|Instituto Baleia Jubarte (Humpback Whale Institute), Caravelas, Brazil.
authors.forename: Kátia R
authors.initials: KR
authors.lastname: Groch
authors.affiliation: University of São Paulo, São Paulo, Brazil.
authors.forename: José Luiz
authors.initials: JL
authors.lastname: Catão-Dias
authors.affiliation: Hawaii Pacific University, Kaneohe, Hawaii, USA.
authors.forename: Kristi
authors.initials: K
authors.lastname: West
authors.affiliation: National Chiayi University, Chiayi, Taiwan.
authors.forename: Kun-Wei
authors.initials: KW
authors.lastname: Chan
mesh_terms: D000818:Animals; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D002591:Cetacea / Q000821:virology*; D006254:Hawaii / Q000453:epidemiology; D018103:Morbillivirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D018185:Morbillivirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000662:veterinary; D009691:Nucleic Acid Denaturation; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013030:Spain / Q000453:epidemiology