title: Hepatocyte Isolation After Laparoscopic Liver Resection.
abstract: Liver tissue obtained from partial hepatectomy is a common source for isolation of primary human hepatocytes. Until now, liver resections were most commonly performed by conventional open surgery. Although the laparoscopic approach is currently emerging in liver surgery, data on the outcome of hepatocyte isolation from laparoscopically resected liver tissue are not available. A total of 22 hepatocyte isolations were performed using the two-step collagenase perfusion technique from October 2015 to March 2016. Liver tissue was obtained from n = 15 open liver resections (OLRs) and n = 7 laparoscopic liver resections (LLRs). Isolation parameters (cell yield, viability, and Percoll survival) were assessed and hepatocyte function (plating efficiency, urea, albumin, and aspartate aminotransferase) was measured over a culture period of 6 days (OLR: n = 13; LLR: n = 3). Total cell yield (OLR: 36.81 ± 6.77 × 10(6) cells/g vs. LLR 16.84 ± 10.66 × 10(6) cells/g, p = 0.0318) as well as viable yield (OLR 31.70 ± 6.05 × 10(6) cells/g vs. LLR 14.70 ± 9.89 × 10(6) cells/g, p = 0.0260) was significantly higher in the OLR group. Subgroup analysis revealed that the worse outcome of isolation of laparoscopically resected liver tissue was associated with right-lateral LLRs, whereas hepatocyte isolation from left-lateral LLRs was as effective as from open surgery. Hepatocyte function did not differ between hepatocytes from openly resected versus left-lateral laparoscopically resected liver tissue. We here present the first data on hepatocyte isolation from laparoscopic liver surgery. Although the overall outcome is worse compared with open surgery, our data suggest that liver tissue from laparoscopic resection of the left lobe is an excellent source for primary human hepatocytes.
authors.affiliation: 1 Department of Surgery, Campus Charité Mitte, Campus Virchow-Klinikum, Experimental Surgery and Regenerative Medicine, Charité-Universitätsmedizin , Berlin, Germany .
authors.forename: Rosa
authors.initials: R
authors.lastname: Horner
authors.affiliation: 1 Department of Surgery, Campus Charité Mitte, Campus Virchow-Klinikum, Experimental Surgery and Regenerative Medicine, Charité-Universitätsmedizin , Berlin, Germany .
authors.forename: Martin
authors.initials: M
authors.lastname: Kluge
authors.affiliation: 1 Department of Surgery, Campus Charité Mitte, Campus Virchow-Klinikum, Experimental Surgery and Regenerative Medicine, Charité-Universitätsmedizin , Berlin, Germany .
authors.forename: Joseph
authors.initials: J
authors.lastname: Gassner
authors.affiliation: 1 Department of Surgery, Campus Charité Mitte, Campus Virchow-Klinikum, Experimental Surgery and Regenerative Medicine, Charité-Universitätsmedizin , Berlin, Germany .
authors.forename: Maximilian
authors.initials: M
authors.lastname: Nösser
authors.affiliation: 1 Department of Surgery, Campus Charité Mitte, Campus Virchow-Klinikum, Experimental Surgery and Regenerative Medicine, Charité-Universitätsmedizin , Berlin, Germany .
authors.forename: Rebeka Dalma
authors.initials: RD
authors.lastname: Major
authors.affiliation: 1 Department of Surgery, Campus Charité Mitte, Campus Virchow-Klinikum, Experimental Surgery and Regenerative Medicine, Charité-Universitätsmedizin , Berlin, Germany .
authors.forename: Anja
authors.initials: A
authors.lastname: Reutzel-Selke
authors.affiliation: 1 Department of Surgery, Campus Charité Mitte, Campus Virchow-Klinikum, Experimental Surgery and Regenerative Medicine, Charité-Universitätsmedizin , Berlin, Germany .
authors.forename: Annekatrin K
authors.initials: AK
authors.lastname: Leder
authors.affiliation: 1 Department of Surgery, Campus Charité Mitte, Campus Virchow-Klinikum, Experimental Surgery and Regenerative Medicine, Charité-Universitätsmedizin , Berlin, Germany .
authors.forename: Benjamin
authors.initials: B
authors.lastname: Struecker
authors.affiliation: 1 Department of Surgery, Campus Charité Mitte, Campus Virchow-Klinikum, Experimental Surgery and Regenerative Medicine, Charité-Universitätsmedizin , Berlin, Germany .
authors.forename: Mehmet H
authors.initials: MH
authors.lastname: Morgul
authors.affiliation: 1 Department of Surgery, Campus Charité Mitte, Campus Virchow-Klinikum, Experimental Surgery and Regenerative Medicine, Charité-Universitätsmedizin , Berlin, Germany .
authors.forename: Johann
authors.initials: J
authors.lastname: Pratschke
authors.affiliation: 1 Department of Surgery, Campus Charité Mitte, Campus Virchow-Klinikum, Experimental Surgery and Regenerative Medicine, Charité-Universitätsmedizin , Berlin, Germany .
authors.forename: Igor M
authors.initials: IM
authors.lastname: Sauer
authors.affiliation: 1 Department of Surgery, Campus Charité Mitte, Campus Virchow-Klinikum, Experimental Surgery and Regenerative Medicine, Charité-Universitätsmedizin , Berlin, Germany .|2 BIH Charité Clinican Scientist Program, Berlin Institute of Health (BIH) , Berlin, Germany .
authors.forename: Nathanael
authors.initials: N
authors.lastname: Raschzok
mesh_terms: D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005260:Female; D006498:Hepatectomy; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D010535:Laparoscopy; D008107:Liver Diseases / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D010477:Perfusion