title: Photochemical Deposition of Silver Nanoparticles on Clays and Exploring Their Antibacterial Activity.
abstract: Photochemical method was used to synthesize silver nanoparticles (AgNPs) in the presence of citrate or clay (SWy-1, SYn-1, and Laponite B) as stabilizers and Lucirin TPO as photoinitiator. During the photochemical synthesis, an appearance of the plasmon absorption band was seen around 400 nm, indicating the formation of AgNPs. X-ray diffraction results suggested that AgNPs prepared in SWy-1 were adsorbed into interlamellar space, and moreover, showed some clay exfoliation. In the case of SYn-1, AgNPs was not intercalated. For the AgNP/Lap B sample, the formation of an exfoliated structure occurred. Transmission electron microscopy revealed the spherical shape of AgNPs for all samples. The particle sizes obtained for AgNP/SWy-1, AgNP/SYn-1, and AgNP/Lap B were 2.6, 5.1, and 3.8 nm, respectively. AgNPs adsorbed on SYn-1 reveal nonuniform size and aggregation of some particles. However, AgNP/SWy-1 and AgNP/Lap B samples are more uniform and have diameters smaller than those prepared with SYn-1. This behavior is due to the ability to exfoliate these clays. The antibacterial activities of pure clays, AgNP/citrate, and AgNP/clays were investigated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). AgNPs in the presence of clays (AgNPs/SYn-1 and AgNPs/SWy-1) showed a lower survival index percentage compared to those obtained for pure clays and AgNPs. The AgNP/SWy-1 sample showed good antibacterial activity against both tested species and the lowest survival index of 3.9 and 4.3 against E. coli and S. aureus, respectively. AgNPs are located in the interlayer region of the SWy-1, which has acid sites. These acidic sites may contribute to the release of Ag(+) ions from the surface of AgNPs. On the other hand, Laponite B and AgNP/Lap B samples did not demonstrate any bactericidal activity.
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo , Caixa Postal 780, 13560-970 São Carlos SP, Brazil.
authors.forename: Patrícia C
authors.initials: PC
authors.lastname: Lombardo
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo , Caixa Postal 780, 13560-970 São Carlos SP, Brazil.
authors.forename: Alessandra L
authors.initials: AL
authors.lastname: Poli
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo , Caixa Postal 780, 13560-970 São Carlos SP, Brazil.
authors.forename: Lucas F
authors.initials: LF
authors.lastname: Castro
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo , Caixa Postal 780, 13560-970 São Carlos SP, Brazil.
authors.forename: Janice R
authors.initials: JR
authors.lastname: Perussi
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo , Caixa Postal 780, 13560-970 São Carlos SP, Brazil.
authors.forename: Carla C
authors.initials: CC
authors.lastname: Schmitt
mesh_terms: D000900:Anti-Bacterial Agents; D004926:Escherichia coli; D053768:Metal Nanoparticles; D012834:Silver; D013211:Staphylococcus aureus
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver
keywords: UV irradiation; antibacterial activity; clay; nanocomposites; silver nanoparticles (AgNPs)