title: Determination of curcumin in biologically active supplements and food spices using a mesofluidic platform with fluorescence detection.
abstract: A mesofluidic platform (MP) with fluorescence detection based on a stepwise injection analysis (SWIA) was used for the determination of curcumin in biologically active supplements and food spices. The main units of the MP are a mixing chamber (MC) and an optical channel with a quartz capillary inside. The MC provides rapid and complete mixing solutions by gas bubbling. The proposed method is based on the new rapid and sensitive reaction of curcumin with a fluorescence reagent - 4-(2,3,3-trimethyl-3H-indolium-1-yl)butane-1-sulfonate (TIBS). The fluorescence intensity of TIBS is greatly quenched in the presence of curcumin in an alkaline medium. The linear range was from 1 to 10µM of curcumin, and the limit of detection, calculated as 3σ of a blank test (n=5), was found to be 0.3µM. The sample throughput was 24h(-1). The proposed method was successfully applied for the determination of curcumin in biologically active supplements and samples of food spices. The obtained data were in good agreement with those measured by a HPLC-UV method.
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, Saint-Petersburg University, St. Petersburg State University, SPbSU, SPbU, 7/9 Universitetskaya Nab., St. Petersburg 199034, Russia. Electronic address: petrovastacy@gmail.com.
authors.forename: Anastasiia
authors.initials: A
authors.lastname: Petrova
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, Saint-Petersburg University, St. Petersburg State University, SPbSU, SPbU, 7/9 Universitetskaya Nab., St. Petersburg 199034, Russia.
authors.forename: Dmitriy
authors.initials: D
authors.lastname: Dar'in
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, Saint-Petersburg University, St. Petersburg State University, SPbSU, SPbU, 7/9 Universitetskaya Nab., St. Petersburg 199034, Russia.
authors.forename: Aleхander
authors.initials: A
authors.lastname: Ivanov
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, Saint-Petersburg University, St. Petersburg State University, SPbSU, SPbU, 7/9 Universitetskaya Nab., St. Petersburg 199034, Russia.
authors.forename: Leonid
authors.initials: L
authors.lastname: Moskvin
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi, Fukuoka 819-0395, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Ishimatsu
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi, Fukuoka 819-0395, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Nakano
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi, Fukuoka 819-0395, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Imato
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, Saint-Petersburg University, St. Petersburg State University, SPbSU, SPbU, 7/9 Universitetskaya Nab., St. Petersburg 199034, Russia.
authors.forename: Andrey
authors.initials: A
authors.lastname: Bulatov
mesh_terms: D003474:Curcumin / Q000032:analysis*; D019587:Dietary Supplements / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D057230:Limit of Detection; D017365:Spices / Q000032:analysis*
chemical_list: D003474:Curcumin
keywords: Biologically active supplements; Curcumin; Flow analysis; Fluorescence; Food spices; Mesofluidic platform; Stepwise injection analysis