title: Differential Virtual Screening (DVS) with Active and Inactive Molecular Models for Finding and Profiling GPCR Modulators: Case of the CCK1 Receptor.
abstract: We discovered a constitutively activating mutation (CAM) V308E for the neurotensin NT1 receptor. Molecular dynamics (MD) performed for the CAM NT1-V308E exhibiting a high spontaneous activity, and for the wild-type NT1 without basal activity, show dramatic conformational changes for the CAM. To test if the two MD models could be valuable active and inactive templates for building molecular models for other class-A GPCR, supposed active and inactive models were built by homology for the cholecystokinin CCK1 receptor. Virtual screening of a corporate library with 250 000 compounds was performed with the two CCK1 models, and a differential virtual screening analysis (DVS), led us to isolate 250 predicted agonists and 250 predicted antagonists. The two sets were merged and the compounds were tested in CCK1 agonist and antagonist cellular assays. An excellent correlation was obtained between predictions and biological results. The effective profiling provided by DVS with active and inactive molecular models, opens new perspectives for finding agonists and antagonists for other class-A GPCR, notably for orphan GPCRs for which no ligands are known.
authors.affiliation: Sanofi-Aventis Recherche & Développement, Centre de Toulouse, 195 Route d'Espagne, 31036 Toulouse, France fax: +33 534632156. constantino.diaz@sanofi-aventis.com.
authors.forename: Constantino
authors.initials: C
authors.lastname: Diaz
authors.affiliation: Sanofi-Aventis Recherche & Développement, Centre de Toulouse, 195 Route d'Espagne, 31036 Toulouse, France fax: +33 534632156.
authors.forename: Pascal
authors.initials: P
authors.lastname: Leplatois
authors.affiliation: Sanofi-Aventis Recherche & Développement, Centre de Toulouse, 195 Route d'Espagne, 31036 Toulouse, France fax: +33 534632156.
authors.forename: Patricia
authors.initials: P
authors.lastname: Angelloz-Nicoud
authors.affiliation: Sanofi-Aventis Recherche & Développement, Centre de Toulouse, 195 Route d'Espagne, 31036 Toulouse, France fax: +33 534632156.
authors.forename: Marinette
authors.initials: M
authors.lastname: Lecomte
authors.affiliation: Sanofi-Aventis Recherche & Développement, Centre de Toulouse, 195 Route d'Espagne, 31036 Toulouse, France fax: +33 534632156.
authors.forename: Annick
authors.initials: A
authors.lastname: Josse
authors.affiliation: Sanofi-Aventis Recherche & Développement, Centre de Toulouse, 195 Route d'Espagne, 31036 Toulouse, France fax: +33 534632156.
authors.forename: Monique
authors.initials: M
authors.lastname: Delpech
authors.affiliation: Sanofi-Aventis Recherche & Développement, Centre de Toulouse, 195 Route d'Espagne, 31036 Toulouse, France fax: +33 534632156.
authors.forename: Florence
authors.initials: F
authors.lastname: Pecceu
authors.affiliation: Sanofi-Aventis Recherche & Développement, Centre de Toulouse, 195 Route d'Espagne, 31036 Toulouse, France fax: +33 534632156.
authors.forename: Gérard
authors.initials: G
authors.lastname: Loison
authors.affiliation: Sanofi-Aventis Recherche & Développement, Centre de Toulouse, 195 Route d'Espagne, 31036 Toulouse, France fax: +33 534632156.
authors.forename: David
authors.initials: D
authors.lastname: Shire
authors.affiliation: Sanofi-Aventis Recherche & Développement, Centre de Toulouse, 195 Route d'Espagne, 31036 Toulouse, France fax: +33 534632156.
authors.forename: Marc
authors.initials: M
authors.lastname: Pascal
authors.affiliation: Sanofi-Aventis Recherche & Développement, Centre de Toulouse, 195 Route d'Espagne, 31036 Toulouse, France fax: +33 534632156.
authors.forename: Pascual
authors.initials: P
authors.lastname: Ferrara
authors.affiliation: Sanofi-Aventis Recherche & Développement, Centre de Toulouse, 195 Route d'Espagne, 31036 Toulouse, France fax: +33 534632156.
authors.forename: Edgardo
authors.initials: E
authors.lastname: Ferran
keywords: CCK1; Differential analysis; GPCR; Molecular dynamics; Molecular modeling; Mutagenesis; NT1; Profiling; Virtual screening