title: The Expression Levels of XLF and Mutant P53 Are Inversely Correlated in Head and Neck Cancer Cells.
abstract: XRCC4-like factor (XLF), also known as Cernunnos, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. In this study, we have found that XLF is over-expressed in HPV(+) versus HPV(-) head and neck squamous cell carcinoma (HNSCC) and significantly down-regulated in the HNSCC cell lines expressing high level of mutant p53 protein versus those cell lines harboring wild-type TP53 gene with low p53 protein expression. We have also demonstrated that Werner syndrome protein (WRN), a member of the NHEJ repair pathway, binds to both mutant p53 protein and NHEJ1 gene promoter, and siRNA knockdown of WRN leads to the inhibition of XLF expression in the HNSCC cells. Collectively, these findings suggest that WRN and p53 are involved in the regulation of XLF expression and the activity of WRN might be affected by mutant p53 protein in the HNSCC cells with aberrant TP53 gene mutations, due to the interaction of mutant p53 with WRN. As a result, the expression of XLF in these cancer cells is significantly suppressed. Our study also suggests that XLF is over-expressed in HPV(+) HNSCC with low expression of wild type p53, and might serve as a potential biomarker for HPV(+) HNSCC. Further studies are warranted to investigate the mechanisms underlying the interactive role of WRN and XLF in NHEJ repair pathway.
authors.affiliation: 1. School of Dentistry and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA; 2. North Hospital, Shenyang, China.
authors.forename: Sizhe
authors.initials: S
authors.lastname: Feng
authors.affiliation: 1. School of Dentistry and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA.
authors.forename: Ramin
authors.initials: R
authors.lastname: Rabii
authors.affiliation: 2. North Hospital, Shenyang, China.
authors.forename: Guobiao
authors.initials: G
authors.lastname: Liang
authors.affiliation: 1. School of Dentistry and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA.
authors.forename: Chenxi
authors.initials: C
authors.lastname: Song
authors.affiliation: 1. School of Dentistry and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: 1. School of Dentistry and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA.
authors.forename: Mian
authors.initials: M
authors.lastname: Guo
authors.affiliation: 2. North Hospital, Shenyang, China.
authors.forename: Xuezhong
authors.initials: X
authors.lastname: Wei
authors.affiliation: 1. School of Dentistry and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA.
authors.forename: Diana
authors.initials: D
authors.lastname: Messadi
authors.affiliation: 1. School of Dentistry and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA.
authors.forename: Shen
authors.initials: S
authors.lastname: Hu
keywords: HNSCC; HPV; XLF; biomarker; mutant p53