title: Principles of Amnis Imaging Flow Cytometry.
abstract: Over the last decade imaging flow cytometry (IFC) has become an established technique, as evidenced by its use in over 500 peer-reviewed scientific articles. Nevertheless, it is still an emerging technique with an installed base of less than 5 % that of conventional flow cytometers. In parallel with its adoption, the technology has evolved rapidly, increasing in speed, sensitivity, and real-time data analysis capacity by over an order of magnitude since its introduction. This chapter summarizes IFC's basic principles of operation and describes the current state of the art.
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, WA, USA. dbasiji@comcast.net.
authors.forename: David A
authors.initials: DA
authors.lastname: Basiji
mesh_terms: D000818:Animals; D002477:Cells / Q000737:chemistry; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D019044:Image Cytometry / Q000295:instrumentation / Q000379:methods*; D008322:Mammals; D055095:Optics and Photonics
keywords: Flow cytometry; Imaging; Time delay integration