title: Z-DNA Binding Protein Mediates Host Control of Toxoplasma gondii Infection.
abstract: Intrinsic to Toxoplasma gondii infection is the parasite-induced modulation of the host immune response, which ensures establishment of a chronic lifelong infection. This manipulation of the host immune response allows T. gondii to not only dampen the ability of the host to eliminate the parasite but also trigger parasite differentiation to the slow-growing, encysted bradyzoite form. We previously used RNA sequencing (RNA-seq) to profile the transcriptomes of mice and T. gondii during acute and chronic stages of infection. One of the most abundant host transcripts during acute and chronic infection was Z-DNA binding protein 1 (ZBP1). In this study, we determined that ZBP1 functions to control T. gondii growth. In activated macrophages isolated from ZBP1 deletion (ZBP1(-/-)) mice, T. gondii has an increased rate of replication and a decreased rate of degradation. We also identified a novel function for ZBP1 as a regulator of nitric oxide (NO) production in activated macrophages, even in the absence of T. gondii infection. Upon stimulation, T. gondii-infected ZBP1(-/-) macrophages display increased proinflammatory cytokines compared to wild-type macrophages under the same conditions. These in vitro phenotypes were recapitulated in vivo, with ZBP1(-/-) mice having increased susceptibility to oral challenge, higher cyst burdens during chronic infection, and elevated inflammatory cytokine responses. Taken together, these results highlight a role for ZBP1 in assisting host control of T. gondii infection.
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA.
authors.forename: Kelly J
authors.initials: KJ
authors.lastname: Pittman
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Patrick W
authors.initials: PW
authors.lastname: Cervantes
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA ljknoll@wisc.edu.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Knoll
mesh_terms: D000208:Acute Disease; D000818:Animals; D002908:Chronic Disease; D016207:Cytokines / Q000378:metabolism; D043542:DNA, Z-Form; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006790:Host-Parasite Interactions / Q000502:physiology*; D008264:Macrophages / Q000378:metabolism / Q000469:parasitology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000378:metabolism; D017423:Sequence Analysis, RNA; D014122:Toxoplasma / Q000254:growth & development / Q000472:pathogenicity*; D014124:Toxoplasmosis, Animal / Q000276:immunology / Q000382:microbiology*
chemical_list: D016207:Cytokines; D043542:DNA, Z-Form; D004268:DNA-Binding Proteins; D009569:Nitric Oxide