title: Cathelicidin-encoding Lactococcus lactis promotes mucosal repair in murine experimental colitis.
abstract: OBJECTIVE
  The preventive effect of intrarectal administration of mouse cathelicidin (mCRAMP) and oral administration of mCRAMP-encoding Lactococcus lactis (N4I) has been shown in murine experimental colitis. It is pivotal to understand the ability of N4I whether it can promote mucosal repair in existing colitis.
  
  
  METHODS
  Mice with dextran sulfate sodium-induced ulcerative colitis (UC) were treated orally with L. lactis or its transformed strain with or without nisin induction. The body weight, clinical symptoms, and histological changes of colonic tissues were determined. Sulfasalazine was used as a reference drug. Young adult mouse colon cells were used to further elucidate the direct action and possible mechanisms of mCRAMP to promote colonic wound repair.
  
  
  RESULTS
  Results showed that N4I could improve the clinical symptoms, maintain crypt integrity and preserve mucus-secreting layer in colitis animals. The preparation also could prevent cell death and promote cell proliferation. In contrast, effective dose of sulfasalazine only alleviated clinical symptoms but not the mucosal damage and repair in the colon. In vitro study further showed that mCRAMP could directly promote wound repair by accelerating cell migration but not cell proliferation through the GPCR/MAPK pathway.
  
  
  CONCLUSIONS
  mCRAMP-encoding L. lactis could be a potential therapeutic preparation better than the traditional anti-inflammatory agent in the treatment of UC.
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Clover C M
authors.initials: CC
authors.lastname: Wong
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.|Department of Anaesthesia and Intensive Care, The Chinese University of Hong Kong, Hong Kong, China.|Institute of Digestive Disease, Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Anaesthesia and Intensive Care, The Chinese University of Hong Kong, Hong Kong, China.|Institute of Digestive Disease, Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: William K K
authors.initials: WK
authors.lastname: Wu
authors.affiliation: Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shen
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Ruby L Y
authors.initials: RL
authors.lastname: Chan
authors.affiliation: Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Lu
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, China.
authors.forename: Ming X
authors.initials: MX
authors.lastname: Li
authors.affiliation: Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, China.
authors.forename: Long F
authors.initials: LF
authors.lastname: Li
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Shun X
authors.initials: SX
authors.lastname: Ren
authors.affiliation: Central Laboratory, Affiliated Nanshan Hospital, Guangdong Medical College, Shenzhen, Guangdong, 518052, China.
authors.forename: Yi F
authors.initials: YF
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Affiliated Nanshan Hospital, Guangdong Medical College, Shenzhen, Guangdong, 518052, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Chi H
authors.initials: CH
authors.lastname: Cho
mesh_terms: D000284:Administration, Oral; D000285:Administration, Rectal; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000008:administration & dosage* / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D003106:Colon / Q000166:cytology; D004847:Epithelial Cells; D007413:Intestinal Mucosa / Q000473:pathology / Q000502:physiology*; D013294:Lactococcus lactis; D008297:Male; D008807:Mice, Inbred BALB C; D014945:Wound Healing / Q000187:drug effects*; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins
keywords: Lactococcus lactis; cathelicidin; experimental colitis