title: Polymerization amplified SPR-DNA assay on noncovalently functionalized graphene.
abstract: A highly efficient surface plasmon resonance (SPR)-based DNA assay was developed, by employing noncovalently functionalized graphene nanosheets as a substrate, and enzymatic catalysis-induced polymerization as mass relay. The objective of this strategy was manifold: first of all, to sensitize the overall SPR output by in situ optimized electrogeneration of graphene thin-film, which was characterized by atomic force microscopic topography; secondly, to regulate the self-assembly and orientation of biotinylated capture probes on nickel-chelated nitrilotriacetic acid (NTA) scaffolds, that anchored onto graphene-supported pyrenyl derivatives; and lastly, to synergize the signal amplification via real-time conversion of the additive aniline into polyaniline precipitation by horseradish peroxidase-tagged reporters. With this setup, a precise and replicable DNA sensing platform for specific targets was achieved with a detection limit down to femtomolar, thus demonstrating a beneficial exploration and exploitation of two-dimensional nanomaterials as unique SPR infrastructure. The possibility of such ″bottom-up″ architecture mounted with ″top-down″ weight reactor would be most likely extensible and adaptable to protein determinations.
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094 China.
authors.forename: Pei-Xin
authors.initials: PX
authors.lastname: Yuan
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094 China. Electronic address: sydeng@njust.edu.cn.
authors.forename: Sheng-Yuan
authors.initials: SY
authors.lastname: Deng
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094 China.
authors.forename: Chuan-Guang
authors.initials: CG
authors.lastname: Yao
authors.affiliation: Intelligent Microsystem Technology and Engineering Center, School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing, 210094 China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094 China; University of Grenoble, Alpes-CNRS, DCM UMR 5250, Grenoble, F-38000 France.
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094 China. Electronic address: danshan@njust.edu.cn.
authors.forename: Dan
authors.initials: D
authors.lastname: Shan
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D019921:Biotinylation; D004247:DNA / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D058105:Polymerization; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000814:Aniline Compounds; D061925:Immobilized Nucleic Acids; D010087:Oxides; C416807:polyaniline; D006108:Graphite; D004247:DNA; D006735:Horseradish Peroxidase; C023650:aniline
keywords: Biocatalytic polymerization; DNA assay; Electrochemically reduced graphene nanosheets; Pyrene-tethered nitrilotriacetic acid; Surface plasmon resonance