title: A simple and efficient design to improve the detection of biotin-streptavidin interaction with plasmonic nanobiosensors.
abstract: In this manuscript we propose a simple and efficient strategy to improve the sensitivity of localized surface plasmon resonance (LSPR) shift-based biosensors using biotin-streptavidin recognition interaction as a proof-of-concept. Specifically, biotin molecules are immobilized on a low-cost plasmonic LSPR biosensor based on annealed self-assembled spherical gold nanoparticles (AuNSs) and successively incubated with increasing concentrations of streptavidin, achieving a limit of detection (LOD) of 5nM. Interestingly, when the detection is performed by the same biotin-functionalized plasmonic AuNSs substrate but against streptavidin previously conjugated to gold nanorods, the LSPR shift is 26-fold enhanced. Moreover, we confirm these results through numerical simulations and demonstrate that the proposed sensing architecture can operate as transducer not only to confirm the adsorption of bioanalyte but also to provide the chemical identity of the capture and targeted molecules from their vibrational Raman fingerprints. Therefore, we are confident that the development of such plasmonic biosensors that use metallic labels for improving the sensitivity of detection could become highly promising for future point-of-care diagnostic assays, pushing sensitivity towards single-molecule detection limit.
authors.affiliation: Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, Treboniu Laurean Str. 42, Cluj-Napoca, 400271, Romania.
authors.forename: Monica
authors.initials: M
authors.lastname: Focsan
authors.affiliation: Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, Treboniu Laurean Str. 42, Cluj-Napoca, 400271, Romania.
authors.forename: Andreea
authors.initials: A
authors.lastname: Campu
authors.affiliation: Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, Treboniu Laurean Str. 42, Cluj-Napoca, 400271, Romania.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Craciun
authors.affiliation: Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, Treboniu Laurean Str. 42, Cluj-Napoca, 400271, Romania.
authors.forename: Monica
authors.initials: M
authors.lastname: Potara
authors.affiliation: Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, Treboniu Laurean Str. 42, Cluj-Napoca, 400271, Romania.
authors.forename: Cosmin
authors.initials: C
authors.lastname: Leordean
authors.affiliation: Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, Treboniu Laurean Str. 42, Cluj-Napoca, 400271, Romania; Biomolecular Physics Department, Faculty of Physics, Babes-Bolyai University, M Kogalniceanu Str. 1, Cluj-Napoca, 400084, Romania.
authors.forename: Dana
authors.initials: D
authors.lastname: Maniu
authors.affiliation: Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, Treboniu Laurean Str. 42, Cluj-Napoca, 400271, Romania; Biomolecular Physics Department, Faculty of Physics, Babes-Bolyai University, M Kogalniceanu Str. 1, Cluj-Napoca, 400084, Romania. Electronic address: simion.astilean@phys.ubbcluj.ro.
authors.forename: Simion
authors.initials: S
authors.lastname: Astilean
mesh_terms: D000937:Antigen-Antibody Reactions / Q000276:immunology*; D015374:Biosensing Techniques / Q000295:instrumentation; D001710:Biotin / Q000032:analysis / Q000276:immunology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold; D007118:Immunoassay / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000032:analysis / Q000276:immunology*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D001710:Biotin; D006046:Gold; D019809:Streptavidin
keywords: Improved detection; LSPR biosensors; SERS platform