title: Real-time monitoring of amyloid growth in a rigid gel matrix.
abstract: We demonstrate the real-time monitoring of the growth of amyloid-protein aggregates in a semi-rigid gel environment constructed from a 5% w/v gelatin solution. The kinetics of amyloid fibril growth from reduced and carboxy-methylated κ-casein occurring in the gel medium was contrasted against that obtained in a regular solution assay. Aggregation kinetics were recorded using Thioflavin T fluorescence. Transmission electron microscopy was used to confirm the aggregates' existence and morphology. The current demonstration of controlled amyloid growth in a gel environment represents the first step towards development of an experimental model for investigating the role of spatial and medium factors in the kinetics of aggregation-based proteopathies.
authors.affiliation: Research School of Chemistry, Australian National University, Acton ACT 2601, Australia.
authors.forename: Roshan C
authors.initials: RC
authors.lastname: Dalpadado
authors.affiliation: Research School of Chemistry, Australian National University, Acton ACT 2601, Australia.
authors.forename: Hendrik
authors.initials: H
authors.lastname: Maat
authors.affiliation: Research School of Chemistry, Australian National University, Acton ACT 2601, Australia.
authors.forename: John A
authors.initials: JA
authors.lastname: Carver
authors.affiliation: Research School of Chemistry, Australian National University, Acton ACT 2601, Australia; Institute for Protein Research Osaka University, 3-1- Yamada-oka, Suita, Osaka 565-0871, Japan. Electronic address: damien.hall@anu.edu.au.
authors.forename: Damien
authors.initials: D
authors.lastname: Hall
mesh_terms: D000682:Amyloid / Q000737:chemistry* / Q000648:ultrastructure; D000818:Animals; D052160:Benzothiazoles; D002364:Caseins / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D005782:Gels; D006801:Humans; D007700:Kinetics; D066263:Protein Aggregation, Pathological; D013844:Thiazoles / Q000737:chemistry*
chemical_list: D000682:Amyloid; D052160:Benzothiazoles; D002364:Caseins; D005782:Gels; D013844:Thiazoles; C009462:thioflavin T; D005780:Gelatin