title: Fluorous modified magnetic mesoporous silica composites-incorporated fluorous solid-phase extraction for the specific enrichment of N-linked glycans with simultaneous exclusion of proteins.
abstract: Taking advantage of fluorine-fluorine interactions, fluorous solid-phase extraction (FSPE) is emerging as a novel approach in proteomics research. Notably, silica gel bound with perfluoroalkyl groups was applied to the FSPE of N-linked glycans. Based on previous studies, mesoporous silica coated magnetic nanoparticles bound with perfluoroalkyl groups were synthesized for the specific enrichment of N-linked glycans in this study. The magnetic nanoparticles-incorporated FSPE strategy successfully identified 22 N-linked glycans from the OVA digest with a concentration of 0.5μg/μL, and achieved a detection limit of 5ng/μL (with 16 N-linked glycans identified). It also showed good day-to-day reproducibility. Its selectivity towards BSA protein is 1:200 (molar ratio), showing excellent size-exclusion effect. In addition, the present method proved to be effective for the analysis of the human serum digest, opening up new prospect for the identification of glycans and proteins with other post-translational modifications in biological environment.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China. Electronic address: chdeng@fudan.edu.cn.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
mesh_terms: D005461:Fluorine / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification*; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction
chemical_list: D011134:Polysaccharides; D011506:Proteins; D005461:Fluorine; D012822:Silicon Dioxide
keywords: Fluorous derivatization; Fluorous solid-phase extraction; Mass spectrometry; Mesoporous silica; N-linked glycan