title: Effect of strontium ions on calcification of preosteoblasts cultured on porous calcium- and phosphate-containing titanium oxide layers formed by micro-arc oxidation.
abstract: Strontium (Sr) ions were added to calcium- and phosphate-containing porous titanium oxide layers formed by micro-arc oxidation (MAO) of titanium (Ti) substrates to improve their osseointegration. An MC3T3-E1 preosteoblast was used to evaluate the effect of the incorporated Sr species on cell calcification. Similar surface microporous morphologies of the oxide layers were observed for all specimens produced by MAO, while the contents of the incorporated Sr ions increased with increasing Sr concentrations in MAO electrolytes. The calcium- and phosphate-containing porous layers promoted the cell alkaline phosphatase (ALP) activity, while cell calcification was promoted by the Sr addition. In particular, the ALP activity significantly increased after 10 days of culture, and larger areas of calcified deposits were observed for the specimens treated with MAO electrolytes containing 0.15 mol L(-1) of Sr species. The effect of Sr addition on the calcification of the MAO-treated Ti oxide layers was established in this study.
authors.affiliation: Oral Implantology and Regenerative Dental Medicine, Tokyo Medical and Dental University.
authors.forename: Mizuki
authors.initials: M
authors.lastname: Sato
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tsutsumi
authors.forename: Makoto
authors.initials: M
authors.lastname: Shiota
authors.forename: Takao
authors.initials: T
authors.lastname: Hanawa
authors.forename: Shohei
authors.initials: S
authors.lastname: Kasugai
mesh_terms: D002118:Calcium; D020099:Coated Materials, Biocompatible; D006801:Humans; D007477:Ions; D016348:Osseointegration; D010006:Osteoblasts; D010084:Oxidation-Reduction; D010710:Phosphates; D013324:Strontium; D013499:Surface Properties; D014025:Titanium
chemical_list: D020099:Coated Materials, Biocompatible; D007477:Ions; D010710:Phosphates; D014025:Titanium; D002118:Calcium; D013324:Strontium