title: The malfunction of peroxisome has an impact on the oxidative stress sensitivity in Candida albicans.
abstract: The peroxisome plays an essential role in eukaryotic cellular metabolism, including β-oxidation of fatty acids and detoxification of hydrogen peroxide. However, its functions in the important fungal pathogen, C. albicans, remain to be investigated. In this study, we identified a homologue of Saccharomyces cerevisiae peroxisomal protein Pex1 in this pathogen, and explored its functions in stress tolerance. Fluorescence observation revealed that C. albicans Pex1 was localized in the peroxisomes, and its loss led to the defect in peroxisome formation. Interestingly, the pex1Δ/Δ mutant had increased tolerance to oxidative stress, which was neither associated with the Cap1 pathway, nor related to the altered distribution of catalase. However, under oxidative stress, the pex1Δ/Δ mutant showed increased expression of autophagy-related genes, with enhanced cytoplasm-to-vacuole transport and degradation of the autophagy markers Atg8 and Lap41. Moreover, the double mutants pex1Δ/Δatg8Δ/Δ and pex1Δ/Δatg1Δ/Δ, both of which were defective in autophagy and peroxisome formation, showed remarkable attenuated tolerance to oxidative stress. These results indicated that autophagy is involved in resistance to oxidative stress in pex1Δ/Δ mutant. Taken together, this study provides evidence that the peroxisomal protein Pex1 regulates oxidative stress tolerance in an autophagy-dependent manner in C. albicans.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, PR China.
authors.forename: Yulu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, PR China.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, PR China.
authors.forename: Honggang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, PR China.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Jia
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, PR China.
authors.forename: Chenpeng
authors.initials: C
authors.lastname: Xiao
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin, PR China.
authors.forename: Biao
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, PR China.
authors.forename: Laijun
authors.initials: L
authors.lastname: Xing
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, PR China. Electronic address: nklimingchun@163.com.
authors.forename: Mingchun
authors.initials: M
authors.lastname: Li
mesh_terms: D000074582:AAA Proteins; D000074183:ATPases Associated with Diverse Cellular Activities / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D001343:Autophagy / Q000235:genetics / Q000502:physiology; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism; D002176:Candida albicans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D002374:Catalase / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D006861:Hydrogen Peroxide / Q000378:metabolism; D008264:Macrophages / Q000382:microbiology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D009154:Mutation; D018384:Oxidative Stress / Q000502:physiology*; D020675:Peroxisomes / Q000201:enzymology* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D013312:Stress, Physiological; D014617:Vacuoles / Q000378:metabolism; D014774:Virulence
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; C426523:CAP1 protein, Candida albicans; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D006861:Hydrogen Peroxide; D002374:Catalase; D000074582:AAA Proteins; D000074183:ATPases Associated with Diverse Cellular Activities; C511586:PEX1 protein, S cerevisiae
keywords: Autophagy; Cap1; Oxidative stress; Peroxisome; Pex1