title: Human Papillomavirus Genotyping Testing Practice in 2014: Results of a College of American Pathologists National Survey.
abstract: BACKGROUND
  - College of American Pathologists (CAP) surveys are used to establish national benchmarks for laboratories.
  
  
  OBJECTIVE
  - To investigate human papillomavirus (HPV) genotyping testing practice patterns in laboratories in 2014.
  
  
  METHODS
  - Data were analyzed from the CAP HPV Genotyping Practices Supplemental Questionnaire distributed to 749 laboratories participating in the CAP Human Papillomavirus (High Risk) for Cytology Program.
  
  
  RESULTS
  - Six hundred four of 749 laboratories (80.6%) responded to the survey. More laboratories offered HPV genotyping testing and performed in-house HPV genotyping testing as compared to previous surveys. The Roche cobas HPV test was the most commonly used genotyping method (37.0%; 160 of 433), followed by Hologic Aptima HPV16 18/45 (26.1%; 113 of 433) and Hologic Cervista HPV16/18 (14.3%; 62 of 433). Most laboratories (287 of 399; 71.9%) offered HPV genotyping for high-risk HPV cases regardless of Papanicolaou (Pap) test results and patient age; this pattern was more common in laboratories using cobas. The remaining laboratories specifically offered testing to women with a negative Pap test result at age 30 years and older (65.2%, 73 of 112) or all ages (37.5%, 42 of 112). The median reporting rates of HPV16 and/or HPV18 positivity were 20.6%, 25.7%, 21.1%, and 57.4% for women with positive high-risk HPV adjunctive negative Pap results, atypical squamous cells of undermined significance, low-grade squamous intraepithelial lesion, and high-grade squamous lesion, respectively.
  
  
  CONCLUSIONS
  - Human papillomavirus genotyping testing has increased. Roche cobas and Hologic Aptima genotype methods were the most common, and laboratories using cobas usually offered genotyping regardless of Pap test result and age. The data provide a baseline and trend of HPV genotyping test practices in 2014.
authors.affiliation: From the Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania (Dr Zhao); the Department of Pathology, Walter Reed National Military Medical Center, Bethesda, Maryland (Dr Crothers); the Department of Pathology, PeaceHealth Laboratories, Vancouver, Washington (Dr Ghofrani); the Department of Pathology, Ohio State University Medical Center, Columbus (Drs Li and Shen); the Departments of Biostatistics (Ms Souers) and Surveys (Ms Goodrich), College of American Pathologists, Northfield, Illinois; the Department of Clinical Sciences, University of Central Florida, Orlando (Drs Hussain and Davey); the Department of Pathology, University of Kansas Medical Center, Kansas City (Dr Fan); and the Department of Pathology and Laboratory Medicine, Mayo Clinic, Scottsdale, Arizona (Dr Ocal).
authors.forename: Chengquan
authors.initials: C
authors.lastname: Zhao
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Crothers
authors.forename: Mohiedean
authors.initials: M
authors.lastname: Ghofrani
authors.forename: Zaibo
authors.initials: Z
authors.lastname: Li
authors.forename: Rhona J
authors.initials: RJ
authors.lastname: Souers
authors.forename: Mujtaba
authors.initials: M
authors.lastname: Hussain
authors.forename: Fang
authors.initials: F
authors.lastname: Fan
authors.forename: Idris Tolgay
authors.initials: IT
authors.lastname: Ocal
authors.forename: Kelly
authors.initials: K
authors.lastname: Goodrich
authors.forename: Rulong
authors.initials: R
authors.lastname: Shen
authors.forename: Diane D
authors.initials: DD
authors.lastname: Davey
mesh_terms: D000328:Adult; D003695:Delivery of Health Care; D005260:Female; D019538:Health Care Surveys; D061809:Human Papillomavirus DNA Tests / Q000592:standards / Q000639:trends; D052162:Human papillomavirus 16 / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D052161:Human papillomavirus 18 / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000378:metabolism / Q000821:virology; D010338:Pathology, Clinical / Q000379:methods / Q000639:trends; D015995:Prevalence; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D012306:Risk; D012958:Societies, Scientific; D014481:United States / Q000453:epidemiology; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000453:epidemiology / Q000378:metabolism / Q000821:virology; D014626:Vaginal Smears; D000078329:Workforce; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000453:epidemiology / Q000378:metabolism / Q000821:virology
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral