title: Multichannel silk protein/laminin grafts for spinal cord injury repair.
abstract: The physical, chemical, and bioactive cues provided by biomaterials are critical for spinal cord regeneration following injury. In this study, we investigated the bioactivity of a silk-based scaffold for nerve tissue remodeling that featured morphological guidance in the form of ridges as well as bioactive molecules. Multichannel/laminin (LN) silk scaffolds stimulated growth, development, and the extension of primary hippocampal neurons after 7 days of culture in vitro. And then, the multichannel/LN silk scaffolds were implanted into 2-mm-long hemisection defects in Sprague-Dawley rat spinal cords for 70 days to evaluate their bioactivities of spinal cord remolding. Our results demonstrated that animal behavior was significantly improved in the multichannel/LN group, as evaluated by Basso-Beattie-Bresnahan score, whereas the implantation of multichannels and random pores groups resulted in recurring limps. Moreover, histology and immunohistochemical staining revealed an increase in blood vessels and expression of growth associated protein-43 and neurofilament-200 as well as reduced expression of glial fibrillary acidic protein in the multichannel/LN group, which contributed to the rebuilding of spinal cord defects. Thus, multichannel/LN silk scaffolds mediated cell migration, stimulated blood capillary formation, and promoted axonal extension, suggesting the utility of these scaffolds for spinal cord reconstruction. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 3045-3057, 2016.
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123, China.|School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073, China.|Department of Biomedical Engineering, Tufts University, Medford, 02155 Massachusetts.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123, China.|School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073, China.
authors.forename: Shuqin
authors.initials: S
authors.lastname: Yan
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123, China.|School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073, China.
authors.forename: Renchuan
authors.initials: R
authors.lastname: You
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, 02155 Massachusetts.
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qu
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123, China.
authors.forename: Xiufang
authors.initials: X
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123, China. mzli@suda.edu.cn.
authors.forename: Mingzhong
authors.initials: M
authors.lastname: Li
authors.affiliation: Centre for Advanced Materials and Performance Textiles, School of Fashion and Textiles, RMIT University, Melbourne, 3056, Victoria, Australia.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D048091:Guided Tissue Regeneration / Q000379:methods*; D007797:Laminin / Q000737:chemistry*; D009416:Nerve Regeneration; D017207:Rats, Sprague-Dawley; D047011:Silk / Q000737:chemistry*; D013116:Spinal Cord / Q000502:physiology / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D058630:Spinal Cord Regeneration; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D007797:Laminin; D047011:Silk
keywords: laminin; nerve guide; regeneration; silk scaffold; spinal cord injury