title: A subchronic toxicity study of Radix Dipsaci water extract by oral administration in F344 rats.
abstract: Radix Dipsaci, the dried root of Dipsacus asperoides C.Y. Cheng & T.M.Ai, has therapeutic effects on various disorders, and in particular, bone and joint disease. Despite such ethnomedicinal benefits, there is very little information regarding its in vivo toxicity or adverse effects. This study was conducted to evaluate the potential toxicity of the Radix Dipsaci water Extract (RD-wE) by using F344 rats. The RD-wE was administered orally to rats at doses of 0, 125, 250, 500, 1000, and 2000 mg/kg body weight (bw)/day for 13 weeks. During the treatment period there were no mortalities attributed to RD-wE. Moreover, no toxic effects were observed with regard to body weight, clinical pathology (hematology, clinical biochemistry, and urinalysis), and anatomic pathology (gross findings, organ weight, and microscopic examination). The changes related to the treatment were excessive salivation at the mouth and soft feces, observed in male and female rats at 1000 or 2000 mg/kg bw/day, but these were not accompanied by any microscopic correlate or other pathophysiological changes. Based on these results, the oral no-observed-adverse-effect level of the RD-wE was considered to be 2000 mg/kg bw/day in both genders, although the target organs were not determined under the current experimental conditions.
authors.affiliation: Research Center for Toxicologic Pathology, Korea Institute of Toxicology (KIT), 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-343, Republic of Korea.
authors.forename: Ji-Seok
authors.initials: JS
authors.lastname: Han
authors.affiliation: Research Center for Toxicologic Pathology, Korea Institute of Toxicology (KIT), 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-343, Republic of Korea.
authors.forename: Byoung-Seok
authors.initials: BS
authors.lastname: Lee
authors.affiliation: Research Center for General and Applied Toxicology, Korea Institute of Toxicology (KIT), 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-343, Republic of Korea.
authors.forename: So-Ri
authors.initials: SR
authors.lastname: Han
authors.affiliation: Research Center for General and Applied Toxicology, Korea Institute of Toxicology (KIT), 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-343, Republic of Korea.
authors.forename: Hyoung-Yun
authors.initials: HY
authors.lastname: Han
authors.affiliation: Research Center for General and Applied Toxicology, Korea Institute of Toxicology (KIT), 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-343, Republic of Korea.
authors.forename: Moon-Koo
authors.initials: MK
authors.lastname: Chung
authors.affiliation: College of Pharmacy, Catholic University of Daegu, 13-13 Hayang-ro, Hayang-eup, Gyeongsan-si, Gyeongsangbuk-do, 712-702, Republic of Korea.
authors.forename: Byung-Sun
authors.initials: BS
authors.lastname: Min
authors.affiliation: Toxicological Research Division, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Osong Health Technology Administration Complex, 187 Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do, 363-951, Republic of Korea.
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Seok
authors.affiliation: Research Center for Toxicologic Pathology, Korea Institute of Toxicology (KIT), 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-343, Republic of Korea. Electronic address: ybkim@kitox.re.kr.
authors.forename: Yong-Bum
authors.initials: YB
authors.lastname: Kim
mesh_terms: D000284:Administration, Oral; D000818:Animals; D049917:Dipsacaceae / Q000737:chemistry / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000737:chemistry; D005260:Female; D008297:Male; D019055:No-Observed-Adverse-Effect Level; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000633:toxicity*; D018517:Plant Roots; D010946:Plants, Medicinal; D011916:Rats, Inbred F344; D018570:Risk Assessment; D012472:Salivation / Q000187:drug effects; D012737:Sex Factors; D012798:Sialorrhea / Q000139:chemically induced / Q000503:physiopathology; D012997:Solvents / Q000737:chemistry*; D013997:Time Factors; D059550:Toxicity Tests, Subchronic / Q000379:methods*; D014867:Water / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D012997:Solvents; D014867:Water
keywords: Radix Dipsaci; Subchronic toxicity; Traditional medicines