title: A Long Noncoding RNA Regulates Sister Chromatid Cohesion.
abstract: Long noncoding RNAs (lncRNAs) are involved in diverse cellular processes through multiple mechanisms. Here, we describe a previously uncharacterized human lncRNA, CONCR (cohesion regulator noncoding RNA), that is transcriptionally activated by MYC and is upregulated in multiple cancer types. The expression of CONCR is cell cycle regulated, and it is required for cell-cycle progression and DNA replication. Moreover, cells depleted of CONCR show severe defects in sister chromatid cohesion, suggesting an essential role for CONCR in cohesion establishment during cell division. CONCR interacts with and regulates the activity of DDX11, a DNA-dependent ATPase and helicase involved in DNA replication and sister chromatid cohesion. These findings unveil a direct role for an lncRNA in the establishment of sister chromatid cohesion by modulating DDX11 enzymatic activity.
authors.affiliation: Center for Applied Medical Research (CIMA), Department of Gene Therapy and Regulation of Gene Expression, University of Navarra, 55 Pio XII Avenue, 31008 Pamplona, Spain; Institute of Health Research of Navarra (IdiSNA), 31008 Pamplona, Spain.
authors.forename: Francesco P
authors.initials: FP
authors.lastname: Marchese
authors.affiliation: Center for Applied Medical Research (CIMA), Department of Gene Therapy and Regulation of Gene Expression, University of Navarra, 55 Pio XII Avenue, 31008 Pamplona, Spain; Institute of Health Research of Navarra (IdiSNA), 31008 Pamplona, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Grossi
authors.affiliation: Center for Applied Medical Research (CIMA), Department of Gene Therapy and Regulation of Gene Expression, University of Navarra, 55 Pio XII Avenue, 31008 Pamplona, Spain; Institute of Health Research of Navarra (IdiSNA), 31008 Pamplona, Spain.
authors.forename: Oskar
authors.initials: O
authors.lastname: Marín-Béjar
authors.affiliation: Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, NIH Biomedical Research Center, 251 Bayview Boulevard, Baltimore, MD 21224, USA.
authors.forename: Sanjay Kumar
authors.initials: SK
authors.lastname: Bharti
authors.affiliation: Center for Applied Medical Research (CIMA), Department of Gene Therapy and Regulation of Gene Expression, University of Navarra, 55 Pio XII Avenue, 31008 Pamplona, Spain; Institute of Health Research of Navarra (IdiSNA), 31008 Pamplona, Spain.
authors.forename: Ivan
authors.initials: I
authors.lastname: Raimondi
authors.affiliation: Center for Applied Medical Research (CIMA), Department of Gene Therapy and Regulation of Gene Expression, University of Navarra, 55 Pio XII Avenue, 31008 Pamplona, Spain; Institute of Health Research of Navarra (IdiSNA), 31008 Pamplona, Spain.
authors.forename: Jovanna
authors.initials: J
authors.lastname: González
authors.affiliation: Center for Applied Medical Research (CIMA), Department of Gene Therapy and Regulation of Gene Expression, University of Navarra, 55 Pio XII Avenue, 31008 Pamplona, Spain; Institute of Health Research of Navarra (IdiSNA), 31008 Pamplona, Spain.
authors.forename: Dannys Jorge
authors.initials: DJ
authors.lastname: Martínez-Herrera
authors.affiliation: Center for Applied Medical Research (CIMA), Department of Gene Therapy and Regulation of Gene Expression, University of Navarra, 55 Pio XII Avenue, 31008 Pamplona, Spain; Institute of Health Research of Navarra (IdiSNA), 31008 Pamplona, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Athie
authors.affiliation: Center for Applied Medical Research (CIMA), Department of Gene Therapy and Regulation of Gene Expression, University of Navarra, 55 Pio XII Avenue, 31008 Pamplona, Spain; Institute of Health Research of Navarra (IdiSNA), 31008 Pamplona, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Amadoz
authors.affiliation: Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, NIH Biomedical Research Center, 251 Bayview Boulevard, Baltimore, MD 21224, USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Brosh
authors.affiliation: Center for Applied Medical Research (CIMA), Department of Gene Therapy and Regulation of Gene Expression, University of Navarra, 55 Pio XII Avenue, 31008 Pamplona, Spain; Institute of Health Research of Navarra (IdiSNA), 31008 Pamplona, Spain. Electronic address: maitehuarte@unav.es.
authors.forename: Maite
authors.initials: M
authors.lastname: Huarte
mesh_terms: D000072283:A549 Cells; D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D002842:Chromatids / Q000235:genetics / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006367:HeLa Cells; D006801:Humans; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection; D047368:Tumor Burden; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: C000622172:CONCR long non coding RNA, human; D004273:DNA, Neoplasm; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D062085:RNA, Long Noncoding; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D004265:DNA Helicases; C101830:DDX11 protein, human; D053487:DEAD-box RNA Helicases