title: Rufibacter ruber sp. nov., isolated from fragmentary rock.
abstract: A red-pigmented, Gram-stain-negative, rod-shaped, aerobic bacterium, designated strain CCM 8646T, was isolated from stone fragments in James Ross Island, Antarctica. Strain CCM 8646T was able to grow from 10 to 40 °C, in the presence of up to 1 % (w/v) NaCl and at pH 7.0-11.0. Analysis of the 16S rRNA gene sequence placed strain CCM 8646T in the genus Rufibacter with the closest relative being Rufibacter roseus H359T (97.07 % 16S rRNA gene sequence similarity). The digital DNA-DNA hybridization values between strain CCM 8646T and R. roseus H359T were low (21.30±2.34 %). The major quinone was menaquinone MK-7. The polar lipids comprised phosphatidylethanolamine, an unknown aminoglycolipid and six unknown polar lipids. The G+C content of strain CCM 8646T was 51.54 mol%. On the basis of phenotypic, chemotaxonomic and genotyping results, strain CCM 8646T is considered to represent a novel species within the genus Rufibacter, for which the name Rufibacter ruber sp. nov. is proposed. The type strain is CCM 8646T (=LMG 29438T).
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Kamila
authors.initials: K
authors.lastname: Kýrová
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Ivo
authors.initials: I
authors.lastname: Sedláček
authors.affiliation: Section of Molecular Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Roman
authors.initials: R
authors.lastname: Pantůček
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Stanislava
authors.initials: S
authors.lastname: Králová
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Pavla
authors.initials: P
authors.lastname: Holochová
authors.affiliation: Section of Molecular Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Ivana
authors.initials: I
authors.lastname: Mašlaňová
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Staňková
authors.affiliation: Institut für Mikrobiologie, Veterinärmedizinische Universität Wien, Veterinärplatz 1, Wien A-1210, Austria.
authors.forename: Tanita
authors.initials: T
authors.lastname: Kleinhagauer
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Tereza
authors.initials: T
authors.lastname: Gelbíčová
authors.affiliation: Centre Algatech, Institute of Microbiology, Academy of Sciences, Novohradská 237, 379 81, Třeboň, Czech Republic.
authors.forename: Roman
authors.initials: R
authors.lastname: Sobotka
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Švec
authors.affiliation: Institut für Mikrobiologie, Veterinärmedizinische Universität Wien, Veterinärplatz 1, Wien A-1210, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
mesh_terms: D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7