title: Investigation of injection dose and camera integration time on quantifying pharmacokinetics of a Cy5.5-GX1 probe with dynamic fluorescence imaging in vivo.
abstract: The aim of this article is to investigate the influence of a tracer injection dose (ID) and camera integration time (IT) on quantifying pharmacokinetics of Cy5.5-GX1 in gastric cancer BGC-823 cell xenografted mice. Based on three factors, including whether or not to inject free GX1, the ID of Cy5.5-GX1, and the camera IT, 32 mice were randomly divided into eight groups and received 60-min dynamic fluorescence imaging. Gurfinkel exponential model (GEXPM) and Lammertsma simplified reference tissue model (SRTM) combined with a singular value decomposition analysis were used to quantitatively analyze the acquired dynamic fluorescent images. The binding potential (Bp) and the sum of the pharmacokinetic rate constants (SKRC) of Cy5.5-GX1 were determined by the SRTM and EXPM, respectively. In the tumor region, the SKRC value exhibited an obvious trend with change in the tracer ID, but the Bp value was not sensitive to it. Both the Bp and SKRC values were independent of the camera IT. In addition, the ratio of the tumor-to-muscle region was correlated with the camera IT but was independent of the tracer ID. Dynamic fluorescence imaging in conjunction with a kinetic analysis may provide more quantitative information than static fluorescence imaging, especially for a priori information on the optimal ID of targeted probes for individual therapy.
authors.affiliation: Xidian University, Engineering Research Center of Molecular and Neuro Imaging of Ministry of Education and School of Life Science and Technology, 266 Xinglong Section of Xifeng Road, Xi'an 710071, China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Xidian University, Engineering Research Center of Molecular and Neuro Imaging of Ministry of Education and School of Life Science and Technology, 266 Xinglong Section of Xifeng Road, Xi'an 710071, China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Chen
authors.affiliation: Fourth Military Medical University, State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, 127 Changle Road, Xi'an 710032, China.
authors.forename: Jipeng
authors.initials: J
authors.lastname: Yin
authors.affiliation: Fourth Military Medical University, State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, 127 Changle Road, Xi'an 710032, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Kang
authors.affiliation: Fourth Military Medical University, State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, 127 Changle Road, Xi'an 710032, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Wang
authors.affiliation: Xidian University, Engineering Research Center of Molecular and Neuro Imaging of Ministry of Education and School of Life Science and Technology, 266 Xinglong Section of Xifeng Road, Xi'an 710071, China.
authors.forename: Xianghan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Fourth Military Medical University, State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, 127 Changle Road, Xi'an 710032, China.
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Fourth Military Medical University, State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, 127 Changle Road, Xi'an 710032, China.
authors.forename: Kaichun
authors.initials: K
authors.lastname: Wu
authors.affiliation: Xidian University, Engineering Research Center of Molecular and Neuro Imaging of Ministry of Education and School of Life Science and Technology, 266 Xinglong Section of Xifeng Road, Xi'an 710071, China.
authors.forename: Jimin
authors.initials: J
authors.lastname: Liang
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D009369:Neoplasms / Q000000981:diagnostic imaging*; D061848:Optical Imaging
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005456:Fluorescent Dyes