title: Inhibition of T-cell activation attenuates hypertension, TNFα, IL-17, and blood-brain barrier permeability in pregnant rats with angiogenic imbalance.
abstract: Angiogenic imbalance during pregnancy is associated with immune activation, hypertension, increased T cell infiltration, and neurological insults.
  
  
  
  On gestational day (GD) 12, timed-pregnant rats were infused with anti-angiogenic factors sFlt-1 and sEndoglin (4.7 and 7 μg/kg) to create HELLP syndrome via mini-osmotic pumps for 8 days, with a subset of these rats having Orencia (2 mg/kg) infused on GD13. On GD19, blood-brain barrier (BBB) permeability was evaluated via Evan's Blue infusion, blood was collected for T-cell measurements, inflammatory cytokine secretion. Brain tissues were also collected to examine inflammatory cytokine infiltration.
  
  
  
  T-cell attenuation with Orencia decreased circulating CD4(+) and CD8(+) T cells, circulating tumor necrosis factor alpha (TNFα) and IL-17, BBB permeability and significantly decreased biochemical evidence of HELLP compared to untreated HELLP rats.
  
  
  
  These data support the hypothesis that T cells have a critical role in contributing to the pathophysiology that is seen in angiogenic imbalance during pregnancy.
authors.affiliation: Department of Obstetrics & Gynecology, University of Mississippi Medical Center, Jackson, MS, USA.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Bean
authors.affiliation: Department of Obstetrics & Gynecology, University of Mississippi Medical Center, Jackson, MS, USA.
authors.forename: Shauna-Kay
authors.initials: SK
authors.lastname: Spencer
authors.affiliation: Department of Obstetrics & Gynecology, University of Mississippi Medical Center, Jackson, MS, USA.
authors.forename: Teylor
authors.initials: T
authors.lastname: Bowles
authors.affiliation: Department of Pathology, University of Mississippi Medical Center, Jackson, MS, USA.
authors.forename: Patrick B
authors.initials: PB
authors.lastname: Kyle
authors.affiliation: Department of Pharmacology & Toxicology, University of Mississippi Medical Center, Jackson, MS, USA.
authors.forename: Jan M
authors.initials: JM
authors.lastname: Williams
authors.affiliation: Department of Obstetrics & Gynecology, University of Mississippi Medical Center, Jackson, MS, USA.
authors.forename: Jacob
authors.initials: J
authors.lastname: Gibbens
authors.affiliation: Department of Obstetrics & Gynecology, University of Mississippi Medical Center, Jackson, MS, USA. kwallace2@umc.edu.
authors.forename: Kedra
authors.initials: K
authors.lastname: Wallace
mesh_terms: D000069594:Abatacept / Q000627:therapeutic use; D020533:Angiogenesis Inhibitors / Q000008:administration & dosage; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D001812:Blood-Brain Barrier; D002199:Capillary Permeability; D004195:Disease Models, Animal; D000071063:Endoglin / Q000008:administration & dosage; D005260:Female; D017359:HELLP Syndrome / Q000188:drug therapy / Q000276:immunology*; D006801:Humans; D006973:Hypertension; D018836:Inflammation Mediators / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D008213:Lymphocyte Activation; D009389:Neovascularization, Pathologic; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000008:administration & dosage / Q000276:immunology
chemical_list: D020533:Angiogenesis Inhibitors; D000893:Anti-Inflammatory Agents; D000071063:Endoglin; C502004:Eng protein, mouse; D018836:Inflammation Mediators; D020381:Interleukin-17; D014409:Tumor Necrosis Factor-alpha; D000069594:Abatacept; C501163:Flt1 protein, mouse; D040281:Vascular Endothelial Growth Factor Receptor-1
keywords: CD4+ T cells; HELLP syndrome; TNFa; blood-brain barrier; hypertension, pregnancy brain