title: Synthetic redesign of plant lipid metabolism.
abstract: Plant seed lipid metabolism is an area of intensive research, including many examples of transgenic events in which oil composition has been modified. In the selected examples described in this review, progress towards the predictive manipulation of metabolism and the reconstitution of desired traits in a non-native host is considered. The advantages of a particular oilseed crop, Camelina sativa, as a flexible and utilitarian chassis for advanced metabolic engineering and applied synthetic biology are considered, as are the issues that still represent gaps in our ability to predictably alter plant lipid biosynthesis. Opportunities to deliver useful bio-based products via transgenic plants are described, some of which represent the most complex genetic engineering in plants to date. Future prospects are considered, with a focus on the desire to transition to more (computationally) directed manipulations of metabolism.
authors.affiliation: Biological Chemistry and Crop Protection, Rothamsted Research, West Common, Harpenden, AL5 2JQ, UK.
authors.forename: Richard P
authors.initials: RP
authors.lastname: Haslam
authors.affiliation: Biological Chemistry and Crop Protection, Rothamsted Research, West Common, Harpenden, AL5 2JQ, UK.
authors.forename: Olga
authors.initials: O
authors.lastname: Sayanova
authors.affiliation: Centre for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
authors.forename: Hae Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Centre for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
authors.forename: Edgar B
authors.initials: EB
authors.lastname: Cahoon
authors.affiliation: Biological Chemistry and Crop Protection, Rothamsted Research, West Common, Harpenden, AL5 2JQ, UK.
authors.forename: Johnathan A
authors.initials: JA
authors.lastname: Napier
mesh_terms: D001709:Biotechnology / Q000379:methods*; D019607:Brassicaceae / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics / Q000502:physiology; D060847:Metabolic Engineering / Q000379:methods*; D010938:Plant Oils / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D010938:Plant Oils
keywords: fatty acid metabolism; metabolic engineering; oil crops; plant biotechnology; predictive manipulation