title: Synthesis of superparamagnetic carboxymethyl chitosan/sodium alginate nanosphere and its application for immobilizing α-amylase.
abstract: In order to improve catalytic activity, increase recycling times, reduce use cost for enzyme, superparamagnetic carboxymethyl chitosan/sodium alginate nanosphere (SM/CMC/SA) has been synthesized via an improved hydrothermal method, molecular self-assembly technology, electrostatic interaction and amide linkage. Its mean diameter was 65nm, zeta potential was -36.9mV and BET was 53.8m(2)/g. α-Amylase was selected as a simulation object to manufacture an immobilized enzyme (SM/CMC/CA/α-Amy), and its catalytic activity, release behavior, reusability and stability were researched. Immobilization increased 4.67 times to catalytic activity, slowed down release rate and improved reusable performance. SM/CMC/CA/α-Amy showed higher activity over a wider pH range, especially in strong acidic and alkaline environments. The thermal stability and storage stability were improved remarkably too. All these results indicated that SM/CMC/SA was an ideal carrier for immobilizing enzyme.
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi 563000, PR China. Electronic address: jiangjianfang@zmc.edu.cn.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Jiang
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi 563000, PR China.
authors.forename: Yongzheng
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi 563000, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi 563000, PR China.
authors.forename: Baodong
authors.initials: B
authors.lastname: Cui
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi 563000, PR China.
authors.forename: Nanwei
authors.initials: N
authors.lastname: Wan
mesh_terms: D000464:Alginates / Q000737:chemistry*; D055162:Biocatalysis; D060326:Chemistry Techniques, Synthetic; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D059346:Magnets / Q000737:chemistry*; D054118:Nanospheres / Q000737:chemistry*; D000516:alpha-Amylases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; C514968:carboxymethyl-chitosan; D020723:Glucuronic Acid; D048271:Chitosan; D000516:alpha-Amylases
keywords: Carboxymethyl chitosan; Immobilize; Magnetofluid; Sodium alginate; α-Amylase