title: Biosequestration of copper by bacteria isolated from an abandoned mine by using microbially induced calcite precipitation.
abstract: Abandoned mine sites are frequently polluted with high concentrations of heavy metals. In this study, 25 calcite-forming bacteria were newly isolated from the soil of an abandoned metal mine in Korea. Based on their urease activity, calcite production, and resistance to copper toxicity, four isolates were selected and further identified by 16S rRNA gene sequencing. Among the isolates, Sporosarcina soli B-22 was selected for subsequent copper biosequestration studies, using the sand impermeability test by production of calcite and extracellular polymeric substance. High removal rates (61.8%) of copper were obtained when the sand samples were analyzed using an inductively coupled plasma-optical emission spectrometer following 72 h of incubation. Scanning electron microscopy showed that the copper carbonate precipitates had a diameter of approximately 5-10 μm. X-ray diffraction further confirmed the presence of copper carbonate and calcium carbonate crystals.
authors.affiliation: Department of Biological Engineering, Inha University.
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Kang
authors.forename: YuJin
authors.initials: Y
authors.lastname: Shin
authors.forename: Periasamy
authors.initials: P
authors.lastname: Anbu
authors.forename: In-Hyun
authors.initials: IH
authors.lastname: Nam
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: So
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002119:Calcium Carbonate / Q000302:isolation & purification / Q000378:metabolism*; D002254:Carbonates / Q000737:chemistry* / Q000302:isolation & purification; D003300:Copper / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008670:Metals; D008855:Microscopy, Electron, Scanning; D008906:Mining; D012336:RNA, Ribosomal, 16S; D056910:Republic of Korea; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D058644:Sporosarcina / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014510:Urease / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D002254:Carbonates; D008670:Metals; D012336:RNA, Ribosomal, 16S; D012987:Soil; D003300:Copper; C086270:copper carbonate; D014510:Urease; D002119:Calcium Carbonate