title: The anti-bacterial activity of titanium-copper sintered alloy against Porphyromonas gingivalis in vitro.
abstract: This study investigates the anti-bacterial property of Ti-Cu sintered alloys against Porphyromonas gingivalis. The anti-anaerobic property of Ti-Cu sintered alloys against P. gingivalis was investigated by antibacterial activity test, DNA measurement, DAPI staining and morphology observation. The antibacterial rates of the Ti-5Cu against P. gingivalis after 18 and 24 h incubation were 36.04 and 54.39%, and those of Ti-10Cu were 68.69 and 75.39%, which were lower than their anti-aerobic abilities. The concentration of P. gingivalis DNA gradually decreased with the increasing Cu content, which was nearly 50% after 24 h incubation on Ti-10Cu. SEM results showed that the shape of P. gingivalis changed and the bacteria broke apart with the addition of Cu and the extension of the culture time. Ti-Cu sintered alloys could not only kill anaerobic bacteria but also reduce the activity of the survived bacteria. The anti-anaerobic mechanism was thought to be in associated with the Cu ion released from Ti-Cu alloy.
authors.affiliation: Department of Prosthodontics, School of Stomatology, China Medical University.
authors.forename: Bing
authors.initials: B
authors.lastname: Bai
authors.forename: Erlin
authors.initials: E
authors.lastname: Zhang
authors.forename: Junchao
authors.initials: J
authors.lastname: Liu
authors.forename: Jingtao
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000900:Anti-Bacterial Agents; D003300:Copper; D003722:Dental Alloys; D008422:Materials Testing; D016966:Porphyromonas gingivalis; D014025:Titanium
chemical_list: D000900:Anti-Bacterial Agents; D003722:Dental Alloys; D003300:Copper; D014025:Titanium