title: l-Alpha Glycerylphosphorylcholine as a Potential Radioprotective Agent in Zebrafish Embryo Model.
abstract: This work establishes the zebrafish embryo model for ionizing radiation (IR) modifier research and also evaluates the protective effect of l-alpha glycerylphosphorylcholine (GPC). Embryos were exposed to a single-fraction whole-body gamma irradiation (5, 10, 15, and 20 Gy) at different postfertilization time points and were serially assessed for viability and macro- and micromorphologic abnormalities. After toxicity evaluation, 194 μM of GPC was added for certain groups with 3-h incubation before the radiation. Nuclear factor kappa B (NF-κB) and interleukin-1β (IL-1β) expression changes were measured using quantitative real-time polymerase chain reaction. A higher sensitivity could be observed at earlier stages of the embryogenesis. The lethal dose (LD50) for 6 hours postfertilization (hpf) embryos was 15 Gy and for 24 hpf was 20 Gy on day 7, respectively. GPC administration resulted in a significant improvement in both the distortion rate and survival of the 24 hpf embryos. Qualitative evaluation of the histological changes confirmed the protective effect of GPC. IL-1β and NF-κB overexpression due to 10 Gy irradiation was also reduced by GPC. GPC exhibited promising radioprotective effects in our zebrafish embryo model, decreasing the irradiation-induced morphological damage and lethality with significant reduction of IR-caused pro-inflammatory activation.
authors.affiliation: 1 Attosecond Light Pulse Source, ELI-HU Nonprofit Ltd., Szeged, Hungary .
authors.forename: Emília Rita
authors.initials: ER
authors.lastname: Szabó
authors.affiliation: 2 MTA-SE NAP B Cognitive Translational Behavioural Pharmacology Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University , Budapest, Hungary .
authors.forename: Imola
authors.initials: I
authors.lastname: Plangár
authors.affiliation: 1 Attosecond Light Pulse Source, ELI-HU Nonprofit Ltd., Szeged, Hungary .|3 Institute of Surgical Research, University of Szeged , Szeged, Hungary .
authors.forename: Tünde
authors.initials: T
authors.lastname: Tőkés
authors.affiliation: 4 Department of Oncotherapy, University of Szeged , Szeged, Hungary .
authors.forename: Imola
authors.initials: I
authors.lastname: Mán
authors.affiliation: 1 Attosecond Light Pulse Source, ELI-HU Nonprofit Ltd., Szeged, Hungary .
authors.forename: Róbert
authors.initials: R
authors.lastname: Polanek
authors.affiliation: 5 Institute of Aquaculture and Environmental Safety, Szent István University of Gödöllő , Gödöllő, Hungary .
authors.forename: Róbert
authors.initials: R
authors.lastname: Kovács
authors.affiliation: 4 Department of Oncotherapy, University of Szeged , Szeged, Hungary .
authors.forename: Gábor
authors.initials: G
authors.lastname: Fekete
authors.affiliation: 1 Attosecond Light Pulse Source, ELI-HU Nonprofit Ltd., Szeged, Hungary .|4 Department of Oncotherapy, University of Szeged , Szeged, Hungary .
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szabó
authors.affiliation: 5 Institute of Aquaculture and Environmental Safety, Szent István University of Gödöllő , Gödöllő, Hungary .
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Csenki
authors.affiliation: 6 Department of Pathology and Forensic Veterinary Medicine, Szent István University , Budapest, Hungary .
authors.forename: Ferenc
authors.initials: F
authors.lastname: Baska
authors.affiliation: 1 Attosecond Light Pulse Source, ELI-HU Nonprofit Ltd., Szeged, Hungary .|4 Department of Oncotherapy, University of Szeged , Szeged, Hungary .
authors.forename: Katalin
authors.initials: K
authors.lastname: Hideghéty
mesh_terms: D000818:Animals; D004307:Dose-Response Relationship, Radiation; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000528:radiation effects; D005997:Glycerylphosphorylcholine / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000378:metabolism; D007928:Lethal Dose 50; D023421:Models, Animal; D016328:NF-kappa B / Q000378:metabolism; D011839:Radiation, Ionizing; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D015027:Zebrafish / Q000502:physiology*
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D011837:Radiation-Protective Agents; D005997:Glycerylphosphorylcholine
keywords: L-alpha glycerylphosphorylcholine; NFκB; radiobiology; radioprotection; zebrafish