title: A chimeric protein composed of the binding domains of Clostridium perfringens phospholipase C and Trueperella pyogenes pyolysin induces partial immunoprotection in a mouse model.
abstract: Trueperella pyogenes and Clostridium perfringens are two kinds of conditional pathogens frequently associated with wound infections and succeeding lethal complications in various economic livestock. Pyolysin (PLO) and phospholipase C (PLC) are the key virulence factors of these two pathogens, respectively. In our study, a chimeric protein called rPC-PD4, which is composed of the binding regions of PLO and PLC, was synthesized. The toxicity of rPC-PD4 was evaluated. Results revealed that rPC-PD4 is a safe chimeric molecule that can be used to develop vaccines. Immunizing BALB/c mice with rPC-PD4 induced high titers of serum antibodies that could efficiently neutralize the hemolytic activity of recombinant PLO and PLC. After the challenge with T. pyogenes or C. perfringens was performed through the intraperitoneal route, we observed that rPC-PD4 immunization could provide partial immunoprotection and reduce lung, intestine, and liver tissue damage to mice. This work demonstrated the efficacy of the rationally designed rPC-PD4 chimeric protein as a potential vaccine candidate against C. perfringens and T. pyogenes.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P. R. China.
authors.forename: Yunhao
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P. R. China. Electronic address: zhangwenlong@neau.edu.cn.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, P. R. China; National Food Safety and Nutrition Collaborative Innovation Center, Wuxi, Jiangsu 214122, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Bao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P. R. China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P. R. China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Yan
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P. R. China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P. R. China.
authors.forename: Lingxiao
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P. R. China; National Food Safety and Nutrition Collaborative Innovation Center, Wuxi, Jiangsu 214122, P. R. China. Electronic address: jwwang@neau.edu.cn.
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D000193:Actinomycetales Infections / Q000517:prevention & control; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000737:chemistry*; D001427:Bacterial Toxins / Q000737:chemistry*; D001428:Bacterial Vaccines / Q000276:immunology; D002135:Calcium-Binding Proteins; D003015:Clostridium Infections / Q000517:prevention & control; D003016:Clostridium perfringens / Q000201:enzymology*; D004195:Disease Models, Animal; D006460:Hemolysin Proteins / Q000737:chemistry*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D000072417:Protein Domains; D011994:Recombinant Proteins / Q000276:immunology; D010738:Type C Phospholipases / Q000737:chemistry*; D014614:Vaccines, Synthetic
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D002135:Calcium-Binding Proteins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D010738:Type C Phospholipases
keywords: Binding domain; Chimeric protein; Immunoprotection; Phospholipase C; Pyolysin