title: Mitochondria Biogenesis and Bioenergetics Gene Profiles in Isogenic Prostate Cells with Different Malignant Phenotypes.
abstract: Background. The most significant hallmarks of cancer are directly or indirectly linked to deregulated mitochondria. In this study, we sought to profile mitochondria associated genes in isogenic prostate cell lines with different tumorigenic phenotypes from the same patient. Results. Two isogenic human prostate cell lines RC77N/E (nonmalignant cells) and RC77T/E (malignant cells) were profiled for expression of mitochondrial biogenesis and energy metabolism genes by qRT-PCR using the Human Mitochondria and the Mitochondrial Energy Metabolism RT(2) PCR arrays. Forty-seven genes were differentially regulated between the two cell lines. The interaction and regulatory networks of these genes were generated by Ingenuity Pathway Analysis. UCP2 was the most significantly upregulated gene in primary adenocarcinoma cells in the current study. The overexpression of UCP2 upon malignant transformation was further validated using human prostatectomy clinical specimens. Conclusions. This study demonstrates the overexpression of multiple genes that are involved in mitochondria biogenesis, bioenergetics, and modulation of apoptosis. These genes may play a role in malignant transformation and disease progression. The upregulation of some of these genes in clinical samples indicates that some of the differentially transcribed genes could be the potential targets for therapeutic interventions.
authors.affiliation: Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 651 Colley Avenue, Lester Hall Suite 424, Norfolk, VA 23507, USA; Leroy T. Canoles Jr. Cancer Research Center, Eastern Virginia Medical School, 651 Colley Avenue, Lester Hall Suite 424, Norfolk, VA 23507, USA.
authors.forename: Tanya C
authors.initials: TC
authors.lastname: Burch
authors.affiliation: Department of Surgery, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
authors.forename: Johng S
authors.initials: JS
authors.lastname: Rhim
authors.affiliation: Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 651 Colley Avenue, Lester Hall Suite 424, Norfolk, VA 23507, USA; Leroy T. Canoles Jr. Cancer Research Center, Eastern Virginia Medical School, 651 Colley Avenue, Lester Hall Suite 424, Norfolk, VA 23507, USA.
authors.forename: Julius O
authors.identifier: 0000-0003-2854-7510
authors.initials: JO
authors.lastname: Nyalwidhe
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D017209:Apoptosis / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D018450:Disease Progression; D004734:Energy Metabolism / Q000235:genetics; D053263:Gene Regulatory Networks / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000235:genetics*; D011468:Prostatectomy / Q000379:methods; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D015398:Signal Transduction / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics