title: Ablation of STAT3 in the B Cell Compartment Restricts Gammaherpesvirus Latency In Vivo.
abstract: A challenging property of gammaherpesviruses is their ability to establish lifelong persistence. The establishment of latency in B cells is thought to involve active virus engagement of host signaling pathways. Pathogenic effects of these viruses during latency or following reactivation can be devastating to the host. Many cancers, including those associated with members of the gammaherpesvirus family, Kaposi's sarcoma-associated herpesvirus and Epstein-Barr virus, express elevated levels of active host signal transducer and activator of transcription-3 (STAT3). STAT3 is activated by tyrosine phosphorylation in response to many cytokines and can orchestrate effector responses that include proliferation, inflammation, metastasis, and developmental programming. However, the contribution of STAT3 to gammaherpesvirus pathogenesis remains to be completely understood. This is the first study to have identified STAT3 as a critical host determinant of the ability of gammaherpesvirus to establish long-term latency in an animal model of disease. Following an acute infection, murine gammaherpesvirus 68 (MHV68) established latency in resident B cells, but establishment of latency was dramatically reduced in animals with a B cell-specific STAT3 deletion. The lack of STAT3 in B cells did not impair germinal center responses for immunoglobulin (Ig) class switching in the spleen and did not reduce either total or virus-specific IgG titers. Although ablation of STAT3 in B cells did not have a global effect on these assays of B cell function, it had long-term consequences for the viral load of the host, since virus latency was reduced at 6 to 8 weeks postinfection. Our findings establish host STAT3 as a mediator of gammaherpesvirus persistence.
  
  
  
  The insidious ability of gammaherpesviruses to establish latent infections can have detrimental consequences for the host. Identification of host factors that promote viral latency is essential for understanding latency mechanisms and for therapeutic interventions. We provide the first evidence that STAT3 expression is needed for murine gammaherpesvirus 68 to establish latency in primary B cells during an active immune response to infection. STAT3 deletion in B cells does not impair adaptive immune control of the virus, but loss of STAT3 in B cells has a long-lasting impact on viral persistence. These results indicate a potential therapeutic benefit of STAT3 inhibitors for combating gammaherpesvirus latency and, thereby, associated pathologies.
authors.affiliation: Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Sandeep Steven
authors.initials: SS
authors.lastname: Reddy
authors.affiliation: Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Hui-Chen Chang
authors.initials: HC
authors.lastname: Foreman
authors.affiliation: Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Thubten Ozula
authors.initials: TO
authors.lastname: Sioux
authors.affiliation: Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Gee Ho
authors.initials: GH
authors.lastname: Park
authors.affiliation: Department of Molecular Biotechnology and Health Sciences, University of Torino, Turin, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Poli
authors.affiliation: Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Nancy C
authors.initials: NC
authors.lastname: Reich
authors.affiliation: Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA laurie.krug@stonybrook.edu.
authors.forename: Laurie T
authors.initials: LT
authors.lastname: Krug
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000821:virology*; D004195:Disease Models, Animal; D006566:Herpesviridae Infections / Q000821:virology*; D054884:Host-Pathogen Interactions; D051379:Mice; D018144:Rhadinovirus / Q000502:physiology*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D017735:Virus Latency
chemical_list: D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse