title: Quantification of cefazolin in serum and adipose tissue by ultra high performance liquid chromatography-Tandem mass spectrometry (UHPLC-MS/MS): application to a pilot study of obese women undergoing cesarean delivery.
abstract: Higher doses of cefazolin are required in obese patients for preoperative antibiotic prophylaxis, owing to its low lipophilicity. An ultra high performance liquid chromatography-tandem mass spectrometry method was developed to quantify cefazolin in serum and adipose tissue from 6 obese patients undergoing cesarean delivery, and using stable-isotope labeled cefazolin as an internal standard. The method has a 2μg/g lower limit of quantitation. The concentration in adipose tissue was 3.4±1.6μg/mL, which is less than half of the reported minimum inhibitory concentration of 8μg/mL for cefazolin. Serum cefazolin concentrations were more than 30-fold higher than in adipose tissue.
authors.affiliation: The Rady Faculty of Health Sciences, College of Pharmacy, Pharmaceutical Analysis Laboratory, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Ryan
authors.initials: R
authors.lastname: Lillico
authors.affiliation: The Rady Faculty of Health Sciences, College of Pharmacy, Pharmaceutical Analysis Laboratory, University of Manitoba, Winnipeg, Manitoba, Canada; College of Pharmacy, Roseman University of Health Sciences, South Jordan, UT, USA.
authors.forename: Casey L
authors.initials: CL
authors.lastname: Sayre
authors.affiliation: The Rady Faculty of Health Sciences, College of Pharmacy, Pharmaceutical Analysis Laboratory, University of Manitoba, Winnipeg, Manitoba, Canada; Clinical Pharmacology Section, The Max Rady College of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Sitar
authors.affiliation: The Rady Faculty of Health Sciences, College of Pharmacy, Pharmaceutical Analysis Laboratory, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Neal M
authors.initials: NM
authors.lastname: Davies
authors.affiliation: Department of Anesthesia, The Max Rady College of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Cynthia M
authors.initials: CM
authors.lastname: Baron
authors.affiliation: The Rady Faculty of Health Sciences, College of Pharmacy, Pharmaceutical Analysis Laboratory, University of Manitoba, Winnipeg, Manitoba, Canada. Electronic address: ted.lakowski@umanitoba.ca.
authors.forename: Ted M
authors.initials: TM
authors.lastname: Lakowski
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism*; D000328:Adult; D000900:Anti-Bacterial Agents / Q000097:blood / Q000378:metabolism*; D002437:Cefazolin / Q000097:blood / Q000378:metabolism*; D002585:Cesarean Section; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D009765:Obesity / Q000097:blood / Q000378:metabolism*; D010865:Pilot Projects; D011247:Pregnancy; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D002437:Cefazolin
keywords: Adipose tissue; Cefazolin; Cesarean delivery; Mass spectrometry; Stable-isotope internal standard