title: Vanillin attenuates negative effects of ultraviolet A on the stemness of human adipose tissue-derived mesenchymal stem cells.
abstract: Ultraviolet A (UVA) irradiation induces various changes in cell biology. The objective of this study was to determine the effect of vanillin on UVA irradiation-induced damages in the stemness properties of human adipose tissue-derived mesenchymal stem cells (hAMSCs). UVA-antagonizing mechanisms of vanillin were also examined. The results revealed that vanillin attenuated UVA-induced reduction of the proliferative potential and stemness of hAMSCs evidenced by increased proliferative activity in BrdU incorporation assay and upregulation of stemness-related genes (OCT4, NANOG and SOX2) in response to vanillin treatment. UVA-induced reduction in mRNA level of hypoxia-inducible factor (HIF)-1α was significantly recovered by vanillin. In addition, the antagonizing effect of vanillin on UVA was found to be mediated by reduced production of PGE2 through inhibiting JNK and p38 MAPK. Taken together, these findings showed that vanillin could improve the reduced stemness of hAMSCs induced by UVA. The effect of vanillin is mediated by upregulating HIF-1α via inhibiting PGE2-cAMP signaling. Therefore, vanillin might be used as an antagonizing agent to mitigate the effects of UVA.
authors.affiliation: Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, 131-20 Gyunggi Do, Republic of Korea.
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Bio and Chemical Engineering, Hongik University, 300-16 Sejong City, Republic of Korea.
authors.forename: See-Hyoung
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon City, 164-19 Gyunggi Do, Republic of Korea.
authors.forename: Mi Ok
authors.initials: MO
authors.lastname: Kim
authors.affiliation: Labatory of Molecular and Cellular Biology, School of Pharmacy, Sungkyunkwan University, Suwon City, 164-19 Gyunggi Do, Republic of Korea.
authors.forename: Inhwan
authors.initials: I
authors.lastname: Lim
authors.affiliation: Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon City, 164-19 Gyunggi Do, Republic of Korea.
authors.forename: Mingyeong
authors.initials: M
authors.lastname: Kang
authors.affiliation: Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon City, 164-19 Gyunggi Do, Republic of Korea.
authors.forename: Sae Woong
authors.initials: SW
authors.lastname: Oh
authors.affiliation: Skincure Life Science Institute, Seongnam City, 132-16 Gyunggi Do, Republic of Korea.
authors.forename: Kwangseon
authors.initials: K
authors.lastname: Jung
authors.affiliation: Labatory of Molecular and Cellular Biology, School of Pharmacy, Sungkyunkwan University, Suwon City, 164-19 Gyunggi Do, Republic of Korea.
authors.forename: Dong Gyu
authors.initials: DG
authors.lastname: Jo
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Science, Eulji University, Seongnam City, 131-35 Gyunggi Do, Republic of Korea. Electronic address: chiuchun0716@gmail.com.
authors.forename: Il-Hoon
authors.initials: IH
authors.lastname: Cho
authors.affiliation: Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon City, 164-19 Gyunggi Do, Republic of Korea. Electronic address: bioneer@skku.edu.
authors.forename: Jongsung
authors.initials: J
authors.lastname: Lee
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000473:pathology* / Q000528:radiation effects; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001547:Benzaldehydes / Q000494:pharmacology*; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000473:pathology* / Q000528:radiation effects; D010766:Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D001547:Benzaldehydes; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C100058:vanillin
keywords: HIF; Human adipose tissue-derived mesenchymal stem cells; PGE(2); Stemness gene; UVA toxicity; Vanillin