title: The impact of intestinal resection on the immune function of short bowel syndrome patients.
abstract: Short bowel syndrome (SBS) is characterized by a massive intestinal loss after surgery resection. Likewise, disturbances involving the intestine, which represents a complex immune environment, may result in breakdown of homeostasis and altered responses, thus leading to unpredictable clinical outcomes. However, the consequences of bowel resection were poorly investigated until now. Therefore, this study aimed to evaluate the immunological status of SBS-patients. For this purpose, ten subjects and nine healthy controls were evaluated. Along with some metabolic disturbances, the main results showed higher levels of the inflammatory cytokine IL-6 in plasma among SBS-patients. However, there were no differences in the frequency of CD3+, CD3+CD4+or CD3+CD8+T lymphocytes. An augmented frequency in CD4+and CD8+cells producing IFN-γ was also observed in peripheral blood mononuclear cells (PBMC), together with elevated percentage of CD4+cells producing IL-10. No differences were observed in the frequency of total CD4+CD25-, CD4+CD25+lymphocytes nor in the expression of FoxP3 or GITR. Nevertheless, SBS-patients showed higher frequency of the regulatory T cell population CD4+CD25+CD39+cells in PBMC. In conclusion, these data pointed to SBS as an important disturbance that compromises not only the intestinal environment but also negatively influences systemic immune components.
authors.affiliation: Departamento de Imunologia e Bioquímica - Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: turato@usp.br.
authors.forename: Walter Miguel
authors.initials: WM
authors.lastname: Turato
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas - Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: tonsales@fcfrp.usp.br.
authors.forename: Helioswilton
authors.initials: H
authors.lastname: Sales-Campos
authors.affiliation: Departamento de Clinica Médica, Disciplina de Nutrologia - Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: camilabitu@usp.br.
authors.forename: Camila Bitu Moreno
authors.initials: CB
authors.lastname: Braga
authors.affiliation: Departamento de Clinica Médica, Disciplina de Nutrologia - Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: sfreire@fmrp.usp.br.
authors.forename: Selma Freire Carvalho
authors.initials: SF
authors.lastname: Cunha
authors.affiliation: Departamento de Clinica Médica, Disciplina de Nutrologia - Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: ozeenrique@gmail.com.
authors.forename: José Henrique
authors.initials: JH
authors.lastname: Silvah
authors.affiliation: Departamento de Imunologia e Bioquímica - Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: jsdsilva@fmrp.usp.br.
authors.forename: João Santana
authors.initials: JS
authors.lastname: da Silva
authors.affiliation: Departamento de Clinica Médica, Disciplina de Nutrologia - Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: jsmarchi@fmrp.usp.br.
authors.forename: Julio Sergio
authors.initials: JS
authors.lastname: Marchini
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas - Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: cristina@fcfrp.usp.br.
authors.forename: Cristina Ribeiro
authors.initials: CR
authors.lastname: de Barros Cardoso
mesh_terms: D000328:Adult; D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012778:Short Bowel Syndrome / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D055815:Young Adult
chemical_list: C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: Immune response; Intestinal resection; Regulation; Short bowel syndrome