title: Effect of leptin combined with CoCl2 on healing in Sprague Dawley Rat fracture model.
abstract: To evaluate the effect of leptin combined with CoCl2 on rat femur fracture healing. 48 male Sprague Dawley rats were randomly divided into two main groups. Then standardized femur fractures were created to all rats. Control group rats were treated with 0.5 mL physiological saline, and experimental group rats were treated with 5 μg/Kg.d leptin and 15 mg/Kg.d CoCl2 along with 0.5 mL physiological saline for 42 days intraperitoneally. Each main group was divided into three subgroups for each evaluation at second, fourth and sixth weeks, each subgroup included eight rats. The radiological evaluation showed that the fracture healing progress of experimental group was superior to control group from second week. At fourth week, experimental group had better fracture healing progress than control group significantly. Results of biomechanics show the ultimate load (N) and deflection ultimate load (mm) of experimental group was significantly increased than that in control group from fourth week. The present result demonstrated that leptin combined with CoCl2 significantly increased the mRNA expression levels of HIF1A, Vegfa, Runx2, Bmp2, Bglap and Alpl. It suggested that leptin combined with CoCl2 have a positive effect on rat femur fracture healing by activating the HIF1A pathway.
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Kuo
authors.initials: K
authors.lastname: Xia
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Liyang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D003035:Cobalt / Q000008:administration & dosage* / Q000494:pharmacology; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D005264:Femoral Fractures / Q000188:drug therapy* / Q000235:genetics; D017102:Fracture Healing / Q000187:drug effects*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics; D007274:Injections, Intraperitoneal; D020738:Leptin / Q000008:administration & dosage* / Q000494:pharmacology; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D020738:Leptin; C493716:Runx2 protein, mouse; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D003035:Cobalt; C018021:cobaltous chloride