title: Decompensated liver cirrhosis and neural regulation of mesenteric vascular tone in rats: role of sympathetic, nitrergic and sensory innervations.
abstract: We evaluated the possible alterations produced by liver cholestasis (LC), a model of decompensated liver cirrhosis in sympathetic, sensory and nitrergic nerve function in rat superior mesenteric arteries (SMA). The vasoconstrictor response to electrical field stimulation (EFS) was greater in LC animals. Alpha-adrenoceptor antagonist phentolamine and P2 purinoceptor antagonist suramin decreased this response in LC animals more than in control animals. Both non-specific nitric oxide synthase (NOS) L-NAME and calcitonin gene related peptide (CGRP) (8-37) increased the vasoconstrictor response to EFS more strongly in LC than in control segments. Vasomotor responses to noradrenaline (NA) or CGRP were greater in LC segments, while NO analogue DEA-NO induced a similar vasodilation in both experimental groups. The release of NA was not modified, while those of ATP, nitrite and CGRP were increased in segments from LC. Alpha 1 adrenoceptor, Rho kinase (ROCK) 1 and 2 and total myosin phosphatase (MYPT) expressions were not modified, while alpha 2B adrenoceptor, nNOS expression and nNOS and MYPT phosphorylation were increased by LC. Together, these alterations might counteract the increased splanchnic vasodilation observed in the last phases of decompensated liver cirrhosis.
authors.affiliation: Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, España.|Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, España.
authors.forename: Esther
authors.initials: E
authors.lastname: Sastre
authors.affiliation: Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, España.|Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, España.
authors.forename: Laura
authors.initials: L
authors.lastname: Caracuel
authors.affiliation: Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, España.|Departamento de Cirugía General y Digestiva, Hospital la Paz, Madrid, España.
authors.forename: Isabel
authors.initials: I
authors.lastname: Prieto
authors.affiliation: Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, España.
authors.forename: Pablo
authors.initials: P
authors.lastname: Llévenes
authors.affiliation: Cátedra de Cirugía, Facultad de Medicina, Universidad Complutense de Madrid, España.
authors.forename: M Ángeles
authors.initials: MÁ
authors.lastname: Aller
authors.affiliation: Cátedra de Cirugía, Facultad de Medicina, Universidad Complutense de Madrid, España.
authors.forename: Jaime
authors.initials: J
authors.lastname: Arias
authors.affiliation: Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, España.|Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, España.
authors.forename: Gloria
authors.initials: G
authors.lastname: Balfagón
authors.affiliation: Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, España.|Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, España.
authors.forename: Javier
authors.initials: J
authors.lastname: Blanco-Rivero
mesh_terms: D000818:Animals; D008103:Liver Cirrhosis / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D017538:Mesenteric Artery, Superior / Q000473:pathology / Q000503:physiopathology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology; D054645:Protein Phosphatase 1 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013498:Suramin / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
chemical_list: D013498:Suramin; D052248:Nitric Oxide Synthase Type I; C499850:Ppp1r12a protein, rat; D054645:Protein Phosphatase 1; D019331:NG-Nitroarginine Methyl Ester; D009638:Norepinephrine