title: Immunophenotyping of T cells in the peripheral circulation in psoriasis.
abstract: BACKGROUND
  Psoriasis is a T-helper (Th)-1/Th17-mediated chronic inflammatory disease. Cytokine mediated interaction between T lymphocytes and keratinocytes lead to keratinocyte hyper-proliferation, which leads to further inflammation in the psoriatic plaques. There is an increased population of T-helper cells in the skin lesions as well as in the peripheral circulation in psoriasis. However, the relative percentage of each T-cell phenotype in the disease pathogenesis is understudied. Our aim was to study the immune-phenotype of the different T-helper/T-reg cell subsets in patients with psoriasis, with respect to healthy controls.
  
  
  METHODS
  A total of 189 cases of psoriasis and 189 age- and gender-matched healthy controls were recruited in this cross-sectional study. Disease severity was determined by psoriasis area severity index (PASI) scoring. Peripheral blood mononuclear cells were isolated by Ficoll-Paque density centrifugation, and T-cell immunophenotyping was done by flow cytometric analysis.
  
  
  RESULTS
  In psoriasis, we observed an imbalance in T-cell immunophenotype, characterised by an increase in Th1/Th17 cells and a relative decrease in Th2/T-reg cells, as compared to the healthy controls. We also found that the percentage of Th1/Th17 cells showed a linear trend, increasing with increasing disease severity (PASI).
  
  
  CONCLUSIONS
  Our results suggest an immune-dysregulation in psoriasis associated with a predominance of Th1/Th17 phenotype, especially with increasing severity of the disease.
authors.affiliation: a Department of Biochemistry , Jawaharlal Institute of Post Graduate Medical Education and Research , Puducherry , India.
authors.forename: M
authors.initials: M
authors.lastname: Priyadarssini
authors.affiliation: a Department of Biochemistry , Jawaharlal Institute of Post Graduate Medical Education and Research , Puducherry , India.
authors.forename: D
authors.initials: D
authors.lastname: Divya Priya
authors.affiliation: a Department of Biochemistry , Jawaharlal Institute of Post Graduate Medical Education and Research , Puducherry , India.
authors.forename: S
authors.initials: S
authors.lastname: Indhumathi
authors.affiliation: a Department of Biochemistry , Jawaharlal Institute of Post Graduate Medical Education and Research , Puducherry , India.
authors.forename: Medha
authors.initials: M
authors.lastname: Rajappa
authors.affiliation: b Department of Dermatology , Jawaharlal Institute of Post Graduate Medical Education and Research , Puducherry , India.
authors.forename: Laxmisha
authors.initials: L
authors.lastname: Chandrashekar
authors.affiliation: b Department of Dermatology , Jawaharlal Institute of Post Graduate Medical Education and Research , Puducherry , India.
authors.forename: D M
authors.initials: DM
authors.lastname: Thappa
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000097:blood / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism; D018417:Th1 Cells / Q000378:metabolism; D058504:Th17 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
chemical_list: D016207:Cytokines
keywords: Immunophenotyping; T-cell subsets; T-helper cells; T-reg cells; psoriasis