title: Validation of a Chiral Liquid Chromatographic Method for the Degradation Behavior of Flumequine Enantiomers in Mariculture Pond Water.
abstract: In this work, flumequine (FLU) enantiomers were separated using a Chiralpak OD-H column, with n-hexane-ethanol (20:80, v/v) as the mobile phase at a flow rate of 0.6 mL/min. Solid phase extraction (SPE) was used for cleanup and enrichment. The limit of detection, limit of quantitation, linearity, precision, and intra/interday variation of the chiral high-performance liquid chromatography (HPLC) method were determined. The developed method was then applied to investigate the degradation behavior of FLU enantiomers in mariculture pond water samples. The results showed that the degradation of FLU enantiomers under natural, sterile, or dark conditions was not enantioselective. Chirality 28:649-655, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, P.R. China.
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Wang
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, P.R. China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Gao
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, P.R. China.
authors.forename: Huo-Xi
authors.initials: HX
authors.lastname: Jin
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, P.R. China.
authors.forename: Yang-Guang
authors.initials: YG
authors.lastname: Wang
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, P.R. China.
authors.forename: Wei-Jian
authors.initials: WJ
authors.lastname: Wu
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, P.R. China.
authors.forename: Xiao-Kun
authors.initials: XK
authors.lastname: Ouyang
mesh_terms: D017756:Aquaculture; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D024841:Fluoroquinolones / Q000032:analysis* / Q000737:chemistry*; D061005:Ponds; D052616:Solid Phase Extraction; D013237:Stereoisomerism; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D024841:Fluoroquinolones; D014874:Water Pollutants, Chemical; C012976:flumequine
keywords: chiral analysis; enantioselective behavior; flumequine enantiomer; mariculture pond water