title: Systematic Analysis of Long Noncoding RNAs in the Senescence-accelerated Mouse Prone 8 Brain Using RNA Sequencing.
abstract: Long noncoding RNAs (lncRNAs) may play an important role in Alzheimer's disease (AD) pathogenesis. However, despite considerable research in this area, the comprehensive and systematic understanding of lncRNAs in AD is still limited. The emergence of RNA sequencing provides a predictor and has incomparable advantage compared with other methods, including microarray. In this study, we identified lncRNAs in a 7-month-old mouse brain through deep RNA sequencing using the senescence-accelerated mouse prone 8 (SAMP8) and senescence-accelerated mouse resistant 1 (SAMR1) models. A total of 599,985,802 clean reads and 23,334 lncRNA transcripts were obtained. Then, we identified 97 significantly upregulated and 114 significantly downregulated lncRNA transcripts from all cases in SAMP8 mice relative to SAMR1 mice. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analyses revealed that these significantly dysregulated lncRNAs were involved in regulating the development of AD from various angles, such as nerve growth factor term (GO: 1990089), mitogen-activated protein kinase signaling pathway, and AD pathway. Furthermore, the most probable AD-associated lncRNAs were predicted and listed in detail. Our study provided the systematic dissection of lncRNA profiling in SAMP8 mouse brain and accelerated the development of lncRNA biomarkers in AD. These attracting biomarkers could provide significant insights into AD therapy in the future.
authors.affiliation: Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Beijing, China.|Engineering Research Center of Natural Medicine, Ministry of Education, Beijing Normal University, Beijing, China.|College of Resources Science Technology, Beijing Normal University, Beijing, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Beijing, China.|Engineering Research Center of Natural Medicine, Ministry of Education, Beijing Normal University, Beijing, China.|College of Resources Science Technology, Beijing Normal University, Beijing, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Qin
authors.affiliation: Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Beijing, China.|Engineering Research Center of Natural Medicine, Ministry of Education, Beijing Normal University, Beijing, China.|College of Resources Science Technology, Beijing Normal University, Beijing, China.
authors.forename: Guoqiong
authors.initials: G
authors.lastname: Cao
authors.affiliation: Engineering Research Center of Natural Medicine, Ministry of Education, Beijing Normal University, Beijing, China.|National & Local United Engineering Research Center for Sanqi Resources Protection and Utilization Technology, Kunming, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Xin
authors.affiliation: Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Beijing, China.|Engineering Research Center of Natural Medicine, Ministry of Education, Beijing Normal University, Beijing, China.|College of Resources Science Technology, Beijing Normal University, Beijing, China.
authors.forename: Chengqiang
authors.initials: C
authors.lastname: Feng
authors.affiliation: Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Beijing, China.|Engineering Research Center of Natural Medicine, Ministry of Education, Beijing Normal University, Beijing, China.|College of Resources Science Technology, Beijing Normal University, Beijing, China.|National & Local United Engineering Research Center for Sanqi Resources Protection and Utilization Technology, Kunming, China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Zhang