title: In vitro IFN-α release from IFN-α- and pegylated IFN-α-loaded poly(lactic-co-glycolic acid) and pegylated poly(lactic-co-glycolic acid) nanoparticles.
abstract: Interferon alpha (IFN-α) controlled release of nanoparticles was investigated under in vitro conditions.
  
  
  
  IFN-α and pegylated IFN-α (PEG-IFN-α) were encapsulated by poly(lactic-co-glycolic acid) (PLGA) and pegylated PLGA (PEG-PLGA) copolymers using double emulsion solvent evaporation method.
  
  
  
  The size of resulting four nanoparticles (IFN-α in poly(lactic-co-glycolic acids), IFN-α in poly(lactic-co-glycolic acid)-polyethylene glycol, PEG-IFN-α in poly(lactic-co-glycolic acids) and PEG-IFN-α in poly(lactic-co-glycolic acid)-polyethylene glycol) was below 130 nm diameter. IFN-α encapsulation efficiency of the nanoparticles was between 78 and 91%.
  
  
  
  The in vitro drug release studies conducted in phosphate-buffered saline and human plasma highlighted the role of incubation medium on the IFN release from the nanoparticles. The PEG-IFN-α in poly(lactic-co-glycolic acid)-polyethylene glycol was the most promising nanoparticle among the four formulations because of its remarkably constant release in both phosphate-buffered saline and plasma.
authors.affiliation: Department of Medicine 1, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590, Frankfurt am Main, Germany.|Institute of Materials & Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok Körútja 2., 1117, Budapest, Hungary.|Research Institute of Chemical & Process Engineering, University of Pannonia, Egyetem u. 10, H-8200 Veszprém, Hungary.
authors.forename: Tivadar
authors.initials: T
authors.lastname: Feczkó
authors.affiliation: Institute of Materials & Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok Körútja 2., 1117, Budapest, Hungary.|Research Institute of Chemical & Process Engineering, University of Pannonia, Egyetem u. 10, H-8200 Veszprém, Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fodor-Kardos
authors.affiliation: Department of Haematology, Peterborough City Hospital, Edith Cavell Campus, Bretton Gate Peterborough, PE3 9GZ, Peterborough, UK.
authors.forename: Muttuswamy
authors.initials: M
authors.lastname: Sivakumaran
authors.affiliation: Nanomaterials Research Institute, National Institute of Advanced Industrial Science & Technology, Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
authors.forename: Quazi Tanminul
authors.initials: QT
authors.lastname: Haque Shubhra
mesh_terms: D000998:Antiviral Agents / Q000008:administration & dosage* / Q000097:blood / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000737:chemistry*; D065546:Drug Liberation; D006801:Humans; D007155:Immunologic Factors / Q000008:administration & dosage* / Q000097:blood / Q000737:chemistry; D016898:Interferon-alpha / Q000008:administration & dosage* / Q000097:blood / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000998:Antiviral Agents; D003692:Delayed-Action Preparations; D007155:Immunologic Factors; D016898:Interferon-alpha; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols
keywords: IFN-α; in vitro release; nanoparticles