title: [Role of farnesoid X receptor in rats with acute cholestatic hepatitis].
abstract: OBJECTIVE
  To investigate the role of farnesoid X receptor (FXR) and its downstream molecules small heterodimer partner (SHP), UDP-glucuronosyltransferase 2B4 (UGT2B4), and bile salt export pump (BSEP) in rats with acute cholestatic hepatitis.
  
  
  METHODS
  A total of 20 Sprague-Dawley rats were randomly divided into normal control group and model group, with 10 rats in each group. The rats in the model group were given a single dose (50 mg/kg) ofα-naphthyl isothiocyanate by gavage to establish the animal model of acute cholestatic hepatitis. Quantitative real-time PCR was used to measure the mRNA expression of FXR, UGT2B4, and BSEP in liver tissue at 48 hours after gavage. An automatic biochemical analyzer was used to measure the serum levels of total bilirubin, direct bilirubin, alanine aminotransferase, total bile acid, aspartate aminotransferase, alkaline phosphatase, andγ-glutamyl transferase. The independent samples t-test was used for comparison of means between groups.
  
  
  RESULTS
  The model group had significantly lower mRNA expression of FXR, SHP, UGT2B4, and BSEP in liver tissue than the normal control group (0.152±0.088/0.559±0.194/0.177±0.039/0.561±0.123 vs 1.137±0.215/1.512±0.309/2.394±0.462/1.631±0.376, t = 13.408, 8.260, 15.121, and 8.553, all P < 0.05). The model group had significantly higher liver function parameters than the normal control group (all P < 0.01).
  
  
  CONCLUSIONS
  FXR, SHP, UGT2B4, and BSEP are involved in the development of acute cholestatic hepatitis. Reduced expression of FXR may cause reduced expression of downstream SHP, UGT2B4, and BSEP, increase the synthesis of bile acid, weaken detoxicating and transporting functions, and thus mediate the development of cholestatic hepatitis.
authors.affiliation: Department of Internal Medicine, Wuhan Children's Hospital, Wuhan 430016, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Internal Medicine, Wuhan Children's Hospital, Wuhan 430016, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Xiong
authors.affiliation: Department of Infectious Disease, Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Infectious Disease, Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: H R
authors.initials: HR
authors.lastname: Li
mesh_terms: D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001647:Bile Acids and Salts; D001663:Bilirubin / Q000097:blood; D002779:Cholestasis / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D014453:Glucuronosyltransferase / Q000378:metabolism; D006505:Hepatitis / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D000099150:Receptor, Farnesoid X-Activated
chemical_list: D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters; D000410:Alanine Transaminase; D001647:Bile Acids and Salts; D001663:Bilirubin; D014453:Glucuronosyltransferase; D018160:Receptors, Cytoplasmic and Nuclear; D000099150:Receptor, Farnesoid X-Activated; C490548:Abcb11 protein, rat