title: Biodiesel production from crude jatropha oil catalyzed by immobilized lipase/acyltransferase from Candida parapsilosis in aqueous medium.
abstract: The lipase/acyltransferase from Candida parapsilosis (CpLIP2) immobilized on two synthetic resins (Accurel MP 1000 and Lewatit VP OC 1600) was used as catalyst for the production of biodiesel (fatty acid methyl esters, FAME) by transesterification of jatropha oil with methanol, in a lipid/aqueous system. The oil was dispersed in a buffer solution (pH 6.5) containing methanol in excess (2M in the biphasic system; molar ratio methanol/acyl chains 2:1). Transesterification was carried out at 30°C, under magnetic stirring, using 10% (w/w) of immobilized enzyme in relation to oil. The maximum FAME yields were attained after 8h reaction time: 80.5% and 93.8%, when CpLIP2 immobilized on Accurel MP 1000 or on Lewatit VP OC 1600 were used, respectively. CpLIP2 on both Accurel MP 1000 and Lewatit VP OC 1600 showed high operational stability along 5 consecutive 8h batches.
authors.affiliation: University of Lisbon, Instituto Superior de Agronomia, LEAF, Lisbon, Portugal.
authors.forename: Joana
authors.initials: J
authors.lastname: Rodrigues
authors.affiliation: Montpellier SupAgro, UMR IATE, F-34060 Montpellier, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Perrier
authors.affiliation: CIRAD, UMR IATE, F-34398 Montpellier, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Lecomte
authors.affiliation: Montpellier SupAgro, UMR IATE, F-34060 Montpellier, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Dubreucq
authors.affiliation: University of Lisbon, Instituto Superior de Agronomia, LEAF, Lisbon, Portugal. Electronic address: suzanafdias@mail.telepac.pt.
authors.forename: Suzana
authors.initials: S
authors.lastname: Ferreira-Dias
mesh_terms: D000217:Acyltransferases / Q000737:chemistry; D056804:Biofuels; D002175:Candida / Q000201:enzymology*; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D031286:Jatropha / Q000737:chemistry; D008049:Lipase / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D014867:Water
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D010938:Plant Oils; D014867:Water; D000217:Acyltransferases; D008049:Lipase
keywords: Biodiesel; Candida parapsilosis; CpLIP2; Jatropha oil; Lipase/acyltransferase; Operational stability