title: [Pathological changes in the microstructure of the sensomotor cortex of white rats with experimental subarachnoid hemorrhage and after experimental influences].
abstract: BACKGROUND
  Many works are dedicated to research in the experiment of the cerebrovascular diseases and correction of pathological changes. Among these studies we have not found devoted czuciowo-ruchowej changes cortex of rats subarachnoid hemorrhage.
  
  
  METHODS
  The study was conducted on 18 white rats weighing 220-310 g. The model of subarachnoid hemorrhage (SAH) was created on the base of methodology described by Dudhani RV et al. (2013). SAH develops immediately after injection of autologous arterial blood into the subarachnoid space. Five animals underwent everyday intraperitoneal injection of NO Donator (L-arginine aspartate) for 8 days from the beginning of the experiment. Sampling of the biological material carried on the ninth day after euthanasia of the test animals under anesthesia with diethyl ether.
  
  
  RESULTS
  Swollen, rounded shape neurons were observed while watching the microscope slides of rat brain samples after autologous blood injection. Otherwise, others cells in the same sample looked atrophous, of reduced size, shrunken. There was observed increase in surface area to 623.1 ± 36.0 μm² and volume of neurons to 949.8±78.0 mkm³, significant compared to healthy animals. The severity of pathologic changes was lower in group of therapy by NO donator, namely, the bodies of neurons of the similar size, their shape is almost triangular, most neurons also as macroglial cells have close to normal structure and pericellular zone.
  
  
  CONCLUSIONS
  SAH evokes a number of pathological changes in the rats' brain sensomotor cerebral cortex. Modeling of the SAH leads to significant (p<0,01) morphometric changes in the pyramidal neurons, namely, to increase of the surface of nerve cells in an average up 1.33 times and 1.5 times - of the volume. Therapy by NO donator of animals with model of SAH reduces the severity of cells' lesion, the majority of neurons and macroglial cells have the shape and pericellular spaces close to the normal.
authors.affiliation: Klinika Neurologii i Neurochirurgii, Lwowski Narodowy Uniwersytet Medyczny Imeni Danyla Halitskego, Lwów, Ukraina, address: 79035, Buchmy ul., 22/32, Lviv mob. tel. : 067-934-63-93 e-mail: romaivanova96@gmail.com office. tel. number : (032) 224-91-44.
authors.forename: Andriy
authors.initials: A
authors.lastname: Netlyukh
mesh_terms: D000818:Animals; D009474:Neurons; D051381:Rats; D066191:Sensorimotor Cortex / Q000473:pathology*; D013345:Subarachnoid Hemorrhage / Q000150:complications / Q000473:pathology*; D013346:Subarachnoid Space
keywords: L-arginine aspartate; Subarachnoid hemorrhage (SAH); dystrophic processes; morphometric changes; pyramidal neurons; rats