title: Loss of Cdk5 function in the nucleus accumbens decreases wheel running and may mediate age-related declines in voluntary physical activity.
abstract: Physical inactivity, which drastically increases with advancing age, is associated with numerous chronic diseases. The nucleus accumbens (the pleasure and reward 'hub' in the brain) influences wheel running behaviour in rodents. RNA-sequencing and subsequent bioinformatics analysis led us to hypothesize a potential relationship between the regulation of dendritic spine density, the molecules involved in synaptic transmission, and age-related reductions in wheel running. Upon completion of follow-up studies, we developed the working model that synaptic plasticity in the nucleus accumbens is central to age-related changes in voluntary running. Testing this hypothesis, inhibition of Cdk5 (comprising a molecule central to the processes described above) in the nucleus accumbens reduced wheel running. The results of the present study show that reductions in synaptic transmission and Cdk5 function are related to decreases in voluntary running behaviour and provide guidance for understanding the neural mechanisms that underlie age-dependent reductions in the motivation to be physically active.
  
  
  
  Increases in age are often associated with reduced levels of physical activity, which, in turn, associates with the development of numerous chronic diseases. We aimed to assess molecular differences in the nucleus accumbens (NAc) (a specific brain nucleus postulated to influence rewarding behaviour) with respect to wheel running and sedentary female Wistar rats at 8 and 14 weeks of age. RNA-sequencing was used to interrogate transcriptomic changes between 8- and 14-week-old wheel running rats, and select transcripts were later analysed by quantitative RT-PCR in age-matched sedentary rats. Voluntary wheel running was greatest at 8 weeks and had significantly decreased by 12 weeks. From 619 differentially expressed mRNAs, bioinformatics suggested that cAMP-mediated signalling, dopamine- and cAMP-regulated neuronal phosphoprotein of 32 kDa feedback, and synaptic plasticity were greater in 8- vs. 14-week-old rats. In depth analysis of these networks showed significant (∼20-30%; P < 0.05) decreases in cell adhesion molecule (Cadm)4 and p39 mRNAs, as well as their proteins from 8 to 14 weeks of age in running and sedentary rats. Furthermore, Cadm4, cyclin-dependent kinase 5 (Cdk5) and p39 mRNAs were significantly correlated with voluntary running distance. Analysis of dendritic spine density in the NAc showed that wheel access increased spine density (P < 0.001), whereas spine density was lower in 14- vs. 8-week-old sedentary rats (P = 0.03). Intriguingly, intra-NAc injection of the Cdk5 inhibitor roscovitine, dose-dependently decreased wheel running. Collectively, these experiments suggest that an age-dependent loss in synaptic function and Cdk5/p39 activity in the NAc may be partially responsible for age-related declines in voluntary running behaviour.
authors.affiliation: Department of Biomedical Sciences, University of Missouri, Columbia, MO, USA.
authors.forename: Gregory N
authors.initials: GN
authors.lastname: Ruegsegger
authors.affiliation: Department of Biomedical Sciences, University of Missouri, Columbia, MO, USA.
authors.forename: Ryan G
authors.initials: RG
authors.lastname: Toedebusch
authors.affiliation: Department of Biomedical Sciences, University of Missouri, Columbia, MO, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Childs
authors.affiliation: Department of Biomedical Sciences, University of Missouri, Columbia, MO, USA.
authors.forename: Kolter B
authors.initials: KB
authors.lastname: Grigsby
authors.affiliation: Department of Biomedical Sciences, University of Missouri, Columbia, MO, USA.|Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, MO, USA.|Department of Nutrition and Exercise Physiology, University of Missouri, Columbia, MO, USA.|Dalton Cardiovascular Research Center, University of Missouri, Columbia, MO, USA.
authors.forename: Frank W
authors.initials: FW
authors.lastname: Booth
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D051360:Cyclin-Dependent Kinase 5 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D005260:Female; D009042:Motivation / Q000502:physiology*; D009043:Motor Activity / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology; D009714:Nucleus Accumbens / Q000502:physiology*; D011687:Purines / Q000494:pharmacology; D017208:Rats, Wistar; D000077546:Roscovitine; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D011687:Purines; D000077546:Roscovitine; D051360:Cyclin-Dependent Kinase 5; C495907:Cdk5 protein, rat
keywords: Cdk5; nucleus accumbens; physical activity; reward; synapse; wheel running