title: Genetic Ablation, Sensitization, and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels.
abstract: Advances in genetic technologies enable the highly selective expression of transgenes in targeted neuronal cell types. Transgene expression can be used to noninvasively ablate, silence or activate neurons, providing a tool to probe their contribution to the control of behavior or physiology. Here, we describe the use of the tetrodotoxin (TTX)-resistant voltage-gated sodium channel Nav1.5 for either sensitizing neurons to depolarizing input, or isolating targeted neurons from surrounding neural activity, and methods for selective neuronal ablation using the bacterial nitroreductase NfsB.
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, 20892, USA.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Horstick
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, 20892, USA.
authors.forename: Kathryn M
authors.initials: KM
authors.lastname: Tabor
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, 20892, USA.
authors.forename: Diana C
authors.initials: DC
authors.lastname: Jordan
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, 20892, USA. burgessha@mail.nih.gov.|NIH, 6 Center Drive, Building 6B, Room 3B308, Bethesda, MD, 20892, USA. burgessha@mail.nih.gov.
authors.forename: Harold A
authors.initials: HA
authors.lastname: Burgess
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D062554:NAV1.5 Voltage-Gated Sodium Channel / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D009601:Nitroreductases / Q000378:metabolism*; D026941:Sodium Channel Blockers / Q000494:pharmacology*; D013779:Tetrodotoxin / Q000494:pharmacology*; D015027:Zebrafish
chemical_list: D062554:NAV1.5 Voltage-Gated Sodium Channel; D026941:Sodium Channel Blockers; D013779:Tetrodotoxin; D009601:Nitroreductases
keywords: Ablation; Isolation; Nav1.5; NfsB; Nitroreductase; SCN5a; Sensitization; Silencing; Tetrodotoxin; Zebrafish