title: PELI1 expression is correlated with MYC and BCL6 expression and associated with poor prognosis in diffuse large B-cell lymphoma.
abstract: PELI is a family of E3 ubiquitin ligases that regulate protein activity through a post-translational modification, ubiquitination. While PELI1 has been found to play a pivotal role in inflammatory processes through the activation of Toll-like receptor signaling and the NF-kB pathway, the role of PELI1 in oncogenesis has not been the subject of much investigation. We aimed to explore PELI1 expression in various malignant lymphomas and identify clinicopathologic significance. Immunohistochemistry for PELI1 was performed on a total of 502 cases, including 406 B-cell, 76 T or NK-cell, and 20 Hodgkin lymphomas. High expression of PELI1 was found in high-grade B-cell lymphoma cases such as diffuse large B-cell lymphoma, Burkitt lymphoma, and plasmablastic lymphoma, whereas low-grade B-cell lymphoma, T/NK-cell lymphoma, and Hodgkin lymphoma cases showed very low levels of expression. In vitro cell line studies, the results of western blot, and RT-PCR were concordant with those of the immunohistochemical results; RL7, Pfeiffer, SUDHL-2, DOHH2, and Ramos cell lines showed high levels of PELI1 protein and mRNA expression. In 182 diffuse large B-cell lymphoma, PELI1 expression was positively correlated with the expression of MYC, BCL6, BCL2, and MUM1 (Spearman's ρ=0.427, 0.507, 0.246, and 0.137, respectively; P<0.001, <0.001, 0.001, and 0.066, respectively). In diffuse large B-cell lymphoma, high expression of PELI1 was associated with frequent bone marrow involvement (P=0.013) and shorter relapse-free survival (P=0.002). Our results suggest that PELI1 might participate in B-cell maturation or oncogenic activation of aggressive B-cell lymphomas, both during and after germinal center stages.
authors.affiliation: Department of Pathology, Hallym University Sacred Heart Hospital, Anyang, Republic of Korea.|Department of Pathology, College of Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Ji-Young
authors.identifier: 0000-0001-7967-8487
authors.initials: JY
authors.lastname: Choe
authors.affiliation: Department of Pathology, Seoul National University Boramae Hospital, Seoul, Republic of Korea.
authors.forename: Mira
authors.initials: M
authors.lastname: Park
authors.affiliation: Department of Pathology, College of Medicine, Seoul National University, Seoul, Republic of Korea.|Department of Pathology, Green Cross Laboratories, Yongin, Republic of Korea.
authors.forename: Ji Yun
authors.initials: JY
authors.lastname: Yun
authors.affiliation: Department of Pathology, College of Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Na
authors.affiliation: Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Heounjeong
authors.identifier: 0000-0003-0412-8709
authors.initials: H
authors.lastname: Go
authors.affiliation: Department of Pathology, Inje University Sanggye Paik Hospital, Seoul, Republic of Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Biostatistics, Seoul National University Boramae Hospital, Seoul, Republic of Korea.
authors.forename: Sohee
authors.initials: S
authors.lastname: Oh
authors.affiliation: Department of Pathology, College of Medicine, Seoul National University, Seoul, Republic of Korea.|Department of Pathology, Seoul National University Boramae Hospital, Seoul, Republic of Korea.
authors.forename: Ji Eun
authors.identifier: 0000-0002-2156-9875
authors.initials: JE
authors.lastname: Kim
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002648:Child; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D016403:Lymphoma, Large B-Cell, Diffuse / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis*; D011379:Prognosis; D016016:Proportional Hazards Models; D051560:Proto-Oncogene Proteins c-bcl-6 / Q000096:biosynthesis; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis*; D055815:Young Adult
chemical_list: C084103:BCL6 protein, human; D014408:Biomarkers, Tumor; C489427:MYC protein, human; D009687:Nuclear Proteins; D051560:Proto-Oncogene Proteins c-bcl-6; D016271:Proto-Oncogene Proteins c-myc; C485959:PELI1 protein, human; D044767:Ubiquitin-Protein Ligases