title: Folate-PEG-decorated docetaxel lipid nanoemulsion for improved antitumor activity.
abstract: To develop a folate-based docetaxel lipid nanoemulsion (FLNE) for tumor-targeted treatment.
  
  
  
  The docetaxel LNEs were prepared and characterized. In vitro cytotoxic and cell uptake studies were performed. The tissue distribution and targeting of drug were studied by fluorescence imaging and tumor regression in mice.
  
  
  
  The IC50 values of FLNE on cancer cells were significant. The cell uptake studies showed an increase in fluorescence with time. Imaging studies found that FLNE was superior in tumor targeting by 4.81- and 2.08-fold over controls. The tumor regression proved the superiority of FLNEs.
  
  
  
  The folate strategy was superior over PEGylation, albumin and transferrin strategies. The study demonstrated great potential of FLNE as a prospective targeted delivery system.
authors.affiliation: Nanotechnology Research Lab, Department of Pharmaceutics, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana 506009, India.
authors.forename: Syed Muzammil
authors.initials: SM
authors.lastname: Afzal
authors.affiliation: Department of Pharmacology, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana 506009, India.
authors.forename: Mohammad Zubair
authors.initials: MZ
authors.lastname: Shareef
authors.affiliation: National Institute of Pharmaceutical Education & Research, Balanagar, Hyderabad, Telangana 500037, India.
authors.forename: Tummuri
authors.initials: T
authors.lastname: Dinesh
authors.affiliation: Nanotechnology Research Lab, Department of Pharmaceutics, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana 506009, India.
authors.forename: Veerabrahma
authors.initials: V
authors.lastname: Kishan
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D001940:Breast / Q000187:drug effects* / Q000473:pathology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D000077143:Docetaxel; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D004655:Emulsions / Q000737:chemistry; D005260:Female; D005492:Folic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D061986:MCF-7 Cells; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D043823:Taxoids / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D004655:Emulsions; D008055:Lipids; D043823:Taxoids; C552831:poly(ethylene glycol)-folate; D000077143:Docetaxel; D011092:Polyethylene Glycols; D005492:Folic Acid
keywords: fluorescent LNE cell uptake; fluorescent tissue distribution; folate-containing emulsion; improved targeting; noninvasive imaging study; targeted docetaxel lipid emulsion