title: Global transcriptional start site mapping in Geobacter sulfurreducens during growth with two different electron acceptors.
abstract: Geobacter sulfurreducens is an anaerobic soil bacterium that is involved in biogeochemical cycles of elements such as Fe and Mn. Although significant progress has been made in the understanding of the electron transfer processes in G. sulfurreducens, little is known about the regulatory mechanisms involved in their control. To expand the study of gene regulation in G. sulfurreducens, we carried out a genome-wide identification of transcription start sites (TSS) by 5'RACE and by deep RNA sequencing of primary mRNAs in two growth conditions. TSSs were identified along G. sulfurreducens genome and over 50% of them were located in the upstream region of the associated gene, and in some cases we detected genes with more than one TSS. Our global mapping of TSSs contributes with valuable information, which is needed for the study of transcript structure and transcription regulation signals and can ultimately contribute to the understanding of transcription initiation phenomena in G. sulfurreducens.
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Campus Morelos, Av. Universidad 2001, Cuernavaca Morelos, C.P. 62210, México.
authors.forename: Getzabeth
authors.initials: G
authors.lastname: González
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Campus Morelos, Av. Universidad 2001, Cuernavaca Morelos, C.P. 62210, México.
authors.forename: Aurora
authors.initials: A
authors.lastname: Labastida
authors.affiliation: Unidad Universitaria de Secuenciación Masiva y Bioinformática, Instituto de Biotecnología, Universidad Nacional Autónoma de México Campus Morelos, Av. Universidad 2001, Cuernavaca Morelos, C.P. 62210, México.
authors.forename: Verónica
authors.initials: V
authors.lastname: Jímenez-Jacinto
authors.affiliation: Centro de Ciencias Aplicadas y Desarrollo Tecnológico, Universidad Nacional Autónoma de México, Circuito exterior s/n, Ciudad Universitaria, Coyoacán, D.F., C.P. 04510, México.
authors.forename: Leticia
authors.initials: L
authors.lastname: Vega-Alvarado
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Campus Morelos, Av. Universidad 2001, Cuernavaca Morelos, C.P. 62210, México.
authors.forename: Maricela
authors.initials: M
authors.lastname: Olvera
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Campus Morelos, Av. Universidad 2001, Cuernavaca Morelos, C.P. 62210, México.
authors.forename: Enrique
authors.initials: E
authors.lastname: Morett
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Campus Morelos, Av. Universidad 2001, Cuernavaca Morelos, C.P. 62210, México katy@ibt.unam.mx.
authors.forename: Katy
authors.initials: K
authors.lastname: Juárez
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004579:Electron Transport; D004583:Electrons; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D045302:Geobacter / Q000235:genetics* / Q000254:growth & development; D011401:Promoter Regions, Genetic; D017423:Sequence Analysis, RNA; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins
keywords: Geobacter sulfurreducens; RNA; fumarate and Fe(III) reduction; transcriptional start sites