title: Characterization of a Monoclonal Antibody against Syringate Derivatives: Application of Immunochemical Detection of Methyl Syringate in Honey.
abstract: Syringic acid is one of the key skeletal structures of plant-derived chemicals. The derivatives of syringic acid have certain biological functions. In this study, a monoclonal antibody to syringic acid-based phytochemicals was prepared and characterized. The obtained antibody reacted with methyl syringate, syringic acid, and leonurine. Methyl syringate is a characteristic compound found in manuka honey, other honey varieties, and plants. Manuka honey was fractionated using HPLC, and the reactivity of the fractions with the antibody was examined. The antibody reacted with the fraction in which methyl syringate was eluted. The amount of methyl syringate in honeys as estimated by ELISA using the antibody had a good linearity compared with that estimated by HPLC. These results suggest that the antibody is applicable for the immunochemical detection of syringic acid derivatives in plants and foods.
authors.forename: Yoji
authors.initials: Y
authors.lastname: Kato
authors.forename: Rie
authors.initials: R
authors.lastname: Fujinaka
authors.forename: Maki
authors.initials: M
authors.lastname: Juri
authors.forename: Yui
authors.initials: Y
authors.lastname: Yoshiki
authors.forename: Akari
authors.initials: A
authors.lastname: Ishisaka
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Kitamoto
authors.affiliation: Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University , 111 Kuboki, Soja-shi, Okayama 719-1197, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nitta
authors.affiliation: Healthcare Systems, Company, Ltd. , 2-22-8 Chikusa-ku, Nagoya, Aichi 464-0858, Japan.
authors.forename: Hirohito
authors.initials: H
authors.lastname: Ishikawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005504:Food Analysis; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D006722:Honey / Q000032:analysis*; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; C506133:methyl syringate; C013587:leonurine; D005707:Gallic Acid; C001945:syringic acid
keywords: immunochemical detection; manuka honey; methyl syringate; monoclonal antibody; syringic acid