title: The Anti-Rotaviral and Anti-Inflammatory Effects ofHyrtiosandHaliclonaSpecies.
abstract: The marine spongesHyrtiosandHaliclonaspecies, both of which are known to produce secondary bioactive metabolites, were used to extract 1304KO-327 and 1304KO-328. Such secondary metabolites are potentially antibacterial, antiviral, anti-inflammatory, antitumoral, antifungal, and antiplasmodial. In the present study, the effects of 1304KO-327 and 1304KO-328 were studied for their clinical and pathological importance. The cytotoxicity of 1304KO-327 and 1304KO-328 was assessed via MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on HT-29, Caco-2, and Raw 264.7 cells. Rotavirus-infected Caco-2 cells were used to prove the antiviral effects of the marine sponge extracts. The test results cogently proved that the virus-inhibiting effects of the sponge extracts improved with extract concentration. Anti-inflammatory effects of the marine sponge extracts were tested on Lipopolysaccharide-treated Raw 264.7 cells. Nitric oxide and cytokine were produced by treatment of the cells with LPS and the inhibiting effects of the sponge extracts on IL-1β formation were investigated. This study found that the NO production was decreased dose dependently, and IL-1β formation was significantly reduced by the marine sponge extracts.
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 01369, Republic of Korea.
authors.forename: Su-Im
authors.initials: SI
authors.lastname: Koh
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 01369, Republic of Korea.
authors.forename: Hea-Soon
authors.initials: HS
authors.lastname: Shin
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D016207:Cytokines / Q000276:immunology; D047129:Haliclona / Q000737:chemistry*; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000276:immunology; D011161:Porifera / Q000737:chemistry*; D000067996:RAW 264.7 Cells; D012401:Rotavirus / Q000187:drug effects*; D064210:Secondary Metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000998:Antiviral Agents; D016207:Cytokines; D009569:Nitric Oxide
keywords: Haliclona species; Hyrtios species; anti-inflammation; anti-rotaviral; marine sponge