title: Hyperplastic stomatitis and esophagitis in a tortoise (Testudo graeca) associated with an adenovirus infection.
abstract: A 2-year-old female, spur-thighed tortoise (Testudo graeca) was presented with poor body condition (1/5) and weakness. Fecal analysis revealed large numbers of oxyurid-like eggs, and radiographs were compatible with gastrointestinal obstruction. Despite supportive medical treatment, the animal died. At gross examination, an intestinal obstruction was confirmed. Histopathology revealed severe hyperplastic esophagitis and stomatitis with marked epithelial cytomegaly and enormous basophilic intranuclear inclusion bodies. Electron microscopy examination revealed a large number of 60-80 nm, nonenveloped, icosahedral virions arranged in crystalline arrays within nuclear inclusions of esophageal epithelial cells, morphologically compatible with adenovirus-like particles. PCR for virus identification was performed with DNA extracted from formalin-fixed, paraffin-embedded tissues. A nested, consensus pan-adenovirus PCR and sequencing analysis showed a novel adenovirus. According to phylogenetic calculations, it clustered to genus Atadenovirus in contrast with all other chelonian adenoviruses described to date. The present report details the pathologic findings associated with an adenovirus infection restricted to the upper digestive tract.
authors.affiliation: Servei de Diagnòstic de Patologia Veterinària, Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, Spain (Garcia-Morante, Martínez)Hospital Clínic Veterinari, Universitat Autònoma de Barcelona, Bellaterra, Spain (Martorell)Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, Bellaterra, Spain (Garcia-Morante, Costa, Martínez)Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary (Pénzes).
authors.forename: Beatriz
authors.initials: B
authors.lastname: Garcia-Morante
authors.affiliation: Servei de Diagnòstic de Patologia Veterinària, Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, Spain (Garcia-Morante, Martínez)Hospital Clínic Veterinari, Universitat Autònoma de Barcelona, Bellaterra, Spain (Martorell)Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, Bellaterra, Spain (Garcia-Morante, Costa, Martínez)Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary (Pénzes).
authors.forename: Judit J
authors.initials: JJ
authors.lastname: Pénzes
authors.affiliation: Servei de Diagnòstic de Patologia Veterinària, Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, Spain (Garcia-Morante, Martínez)Hospital Clínic Veterinari, Universitat Autònoma de Barcelona, Bellaterra, Spain (Martorell)Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, Bellaterra, Spain (Garcia-Morante, Costa, Martínez)Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary (Pénzes).
authors.forename: Taiana
authors.initials: T
authors.lastname: Costa
authors.affiliation: Servei de Diagnòstic de Patologia Veterinària, Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, Spain (Garcia-Morante, Martínez)Hospital Clínic Veterinari, Universitat Autònoma de Barcelona, Bellaterra, Spain (Martorell)Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, Bellaterra, Spain (Garcia-Morante, Costa, Martínez)Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary (Pénzes).
authors.forename: Jaime
authors.initials: J
authors.lastname: Martorell
authors.affiliation: Servei de Diagnòstic de Patologia Veterinària, Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, Spain (Garcia-Morante, Martínez)Hospital Clínic Veterinari, Universitat Autònoma de Barcelona, Bellaterra, Spain (Martorell)Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, Bellaterra, Spain (Garcia-Morante, Costa, Martínez)Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary (Pénzes) jorge.martinez.martinez@uab.cat.
authors.forename: Jorge
authors.initials: J
authors.lastname: Martínez
mesh_terms: D000257:Adenoviridae Infections / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D000818:Animals; D046808:Atadenovirus / Q000235:genetics / Q000302:isolation & purification*; D003937:Diagnosis, Differential; D004941:Esophagitis / Q000209:etiology / Q000662:veterinary; D005260:Female; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013280:Stomatitis / Q000209:etiology / Q000662:veterinary; D014426:Turtles
keywords: Adenovirus; Testudo graeca; esophagitis; reptiles; stomatitis; tortoises