title: Influence of adenovirus and MVA vaccines on the breadth and hierarchy of T cell responses.
abstract: Viral-vectored vaccines are in clinical development for several infectious diseases where T-cell responses can mediate protection, and responses to sub-dominant epitopes is needed. Little is known about the influence of MVA or adenoviral vectors on the hierarchy of the dominant and sub-dominant T-cell epitopes. We investigated this aspect in mice using a malaria immunogen. Our results demonstrate that the T-cell hierarchy is influenced by the timing of analysis, rather than by the vector after a single immunization, with hierarchy changing over time. Repeated homologous immunization reduced the breadth of responses, while heterologous prime-boost induced the strongest response to the dominant epitope, albeit with only modest response to the sub-dominant epitopes.
authors.affiliation: Oxford Vaccine Group, Department of Paediatrics, University of Oxford and the NIHR Oxford Biomedical Research Centre, Oxford, United Kingdom. Electronic address: christine.rollier@paediatrics.ox.ac.uk.
authors.forename: Christine S
authors.initials: CS
authors.lastname: Rollier
authors.affiliation: The Jenner Institute, University of Oxford, Roosevelt Drive, Oxford OX3 7DQ, United Kingdom.
authors.forename: Adrian V S
authors.initials: AVS
authors.lastname: Hill
authors.affiliation: The Jenner Institute, University of Oxford, Roosevelt Drive, Oxford OX3 7DQ, United Kingdom.
authors.forename: Arturo
authors.initials: A
authors.lastname: Reyes-Sandoval
mesh_terms: D000256:Adenoviridae; D000818:Animals; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D007111:Immunity, Cellular; D007117:Immunization, Secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D019444:Vaccines, DNA; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; C560489:MVA vaccine; D019444:Vaccines, DNA; D014765:Viral Vaccines
keywords: Subdominant T-cell epitopes; T-cell hierarchy; Vaccines; Viral vectors