title: A rapid and ultrasensitive SERRS assay for histidine and tyrosine based on azo coupling.
abstract: A simple and highly sensitive surface-enhanced resonance Raman scattering (SERRS)-based approach coupled with azo coupling reaction has been put forward for quantitative analysis of histidine and tyrosine. The SERRS-based assay is simple and rapid by mixing the azo reaction products with silver nanoparticles (AgNPs) for measurements within 2min. The limits of detection (LODs) of the method are as low as 4.33×10(-11) and 8.80×10(-11)M for histidine and tyrosine, respectively. Moreover, the SERRS fingerprint information specific to corresponding amino acids guarantees the selective detection for the target histidine and tyrosine. The results from serum indicated the potential application of the proposed approach into biological samples. Compared with the methods ever reported, the main advantages of this methodology are simpleness, rapidity without time-consuming separation or pretreatment steps, high sensitivity, selectivity and the potential for determination of other molecules containing imidazole or phenol groups.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Sui
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Bionic Engineering of Ministry of Education, Jilin University, Changchun, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Key Laboratory of Bionic Engineering of Ministry of Education, Jilin University, Changchun, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Cong
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, PR China.
authors.forename: Xiao Xia
authors.initials: XX
authors.lastname: Han
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D006639:Histidine / Q000032:analysis* / Q000097:blood; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D014443:Tyrosine / Q000032:analysis* / Q000097:blood
chemical_list: D012834:Silver; D014443:Tyrosine; D006639:Histidine
keywords: Ag nanoparticles; Azo coupling; Histidine; SERRS; Tyrosine