title: Age-dependent differential expression of death-associated protein 6 (Daxx) in various peripheral tissues and different brain regions of C57BL/6 male mice.
abstract: Death-associated protein 6 (DAXX) is a ubiquitous protein implicated in various cellular processes such as apoptosis, tumorigenesis, development and transcription. The role of DAXX is however ambiguous and many contradictory results regarding its function in apoptosis upon various cellular stresses are described in the literature. In order to have a better understanding of the role of DAXX throughout the entire organism under physiological stress conditions, we have characterized the mRNA levels, protein expression and the proteolytic processing of DAXX in the normal aging process in peripheral organs and brain regions in C57BL/6 male mice. Overall, Daxx mRNA expression decreases with aging in the liver, kidney, heart, cortex and cerebellum. In contrast, an increase is observed in the striatum. The protein expression of DAXX and of its proteolytic fragments increases with aging in the kidney, heart and cortex. In liver and spleen, no changes are observed while in the striatum and cerebellum, certain forms increase and others decrease with age, suggesting that the functions of DAXX may be cell type dependent. This study provides important details regarding the expression and post-translational modifications of DAXX in aging in the entire organism and provides reference data for the deregulation observed in age-associated diseases.
authors.affiliation: Research Centre on Aging, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada.|Department of Pharmacology and Physiology, Faculty of Medicine and Health Sciences, University of Sherbrooke, 3001 12e Avenue Nord, Sherbrooke, QC, J1H 5N4, Canada.
authors.forename: Mélissa
authors.initials: M
authors.lastname: Lessard-Beaudoin
authors.affiliation: Research Centre on Aging, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada.|Department of Pharmacology and Physiology, Faculty of Medicine and Health Sciences, University of Sherbrooke, 3001 12e Avenue Nord, Sherbrooke, QC, J1H 5N4, Canada.
authors.forename: Mélissa
authors.initials: M
authors.lastname: Laroche
authors.affiliation: Research Centre on Aging, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada.|Department of Pharmacology and Physiology, Faculty of Medicine and Health Sciences, University of Sherbrooke, 3001 12e Avenue Nord, Sherbrooke, QC, J1H 5N4, Canada.
authors.forename: Marie-Josée
authors.initials: MJ
authors.lastname: Demers
authors.affiliation: Department of Pharmacology and Physiology, Faculty of Medicine and Health Sciences, University of Sherbrooke, 3001 12e Avenue Nord, Sherbrooke, QC, J1H 5N4, Canada.
authors.forename: Catherine
authors.initials: C
authors.lastname: Duclos
authors.affiliation: Department of Pharmacology and Physiology, Faculty of Medicine and Health Sciences, University of Sherbrooke, 3001 12e Avenue Nord, Sherbrooke, QC, J1H 5N4, Canada.
authors.forename: Jean-Bernard
authors.initials: JB
authors.lastname: Denault
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada.|CHUS Research Centre, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Grenier
authors.affiliation: Max Delbrück Centrum für Molekulare Medizin, Berlin-Buch, 13125, Berlin, Germany.
authors.forename: Sean-Patrick
authors.initials: SP
authors.lastname: Riechers
authors.affiliation: Max Delbrück Centrum für Molekulare Medizin, Berlin-Buch, 13125, Berlin, Germany.
authors.forename: Erich E
authors.initials: EE
authors.lastname: Wanker
authors.affiliation: Research Centre on Aging, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada. Rona.Graham@USherbooke.ca.|Department of Pharmacology and Physiology, Faculty of Medicine and Health Sciences, University of Sherbrooke, 3001 12e Avenue Nord, Sherbrooke, QC, J1H 5N4, Canada. Rona.Graham@USherbooke.ca.
authors.forename: Rona K
authors.initials: RK
authors.lastname: Graham
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D056970:Co-Repressor Proteins; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018832:Molecular Chaperones; D009687:Nuclear Proteins / Q000378:metabolism*; D009928:Organ Specificity / Q000502:physiology; D014781:Viscera / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D056970:Co-Repressor Proteins; C491473:Daxx protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D018832:Molecular Chaperones; D009687:Nuclear Proteins
keywords: Aging; Brain region; C57BL/6 mice; Caspase; DAXX; Peripheral organs