title: Effects of abalone (Haliotis discus hannai Ino) gonad polysaccharides on cholecystokinin release in STC-1 cells and its signaling mechanism.
abstract: Abalone gonad polysaccharide (AGP) -31, -32 and -33 prepared in this study had the molecular weight (MW) of 37.8, 32.2 and 27.5kDa, respectively. They all contained mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, arabinose, and fucose, with very similar monosaccharide profile. All the three polysaccharides could significantly increase the secretion of cholecystokinin (CCK) in STC-1 cells. Among them, AGP-32 showed the strongest effect. However, the low-MW fragments of AGP-32 showed significantly lower activity than AGP-32 itself. It was also found that the inhibitors on calcium-sensing receptor (CaSR), protein kinase A (PKA), Ca(2+)⁄calmodulin-dependent protein kinase (CaMK) II, p38- mitogen-activated protein kinases (MAPK), and an intracellular calcium chelator all inhibited AGP-induced CCK secretion. To conclude, Ca(2+)/calmodulin (CaM)/CaMK, cyclic adenosine monophosphate (cAMP)/PKA and MAPK pathways are all involved in AGP-induced CCK secretion.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China; School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian 116034, PR China.
authors.forename: Da-Yong
authors.initials: DY
authors.lastname: Zhou
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian 116034, PR China.
authors.forename: Jing-Feng
authors.initials: JF
authors.lastname: Yang
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian 116034, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Song
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China.
authors.forename: Ce
authors.initials: C
authors.lastname: Zhu
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Song
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA.
authors.forename: Chen-Xu
authors.initials: CX
authors.lastname: Yu
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China; School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian 116034, PR China. Electronic address: zhubeiwei@163.com.
authors.forename: Bei-Wei
authors.initials: BW
authors.lastname: Zhu
keywords: 3-Phenylphenol (PubChem CID: 11381); Abalone (Haliotis discus hannai Ino) gonad; Alkaline protease (PubChem CID: 72355); BAPTA-AM (PubChem CID: 2293); Cholecystokinin; Enteroendocrine STC-1 cells; H89 (PubChem CID: 449241); KN62 (PubChem CID: 3838); NPS2143 (PubChem CID: 6918446); Polysaccharides; SB203580 (PubChem CID:176155); Signaling pathway; Sodium tetraborate (PubChem CID: 13211214); Trifluoroacetic acid (PubChem CID: 6422)