title: An enzyme-responsive conjugate improves the delivery of a PI3K inhibitor to prostate cancer.
abstract: An enzyme-responsive peptide drug conjugate was developed for TGX-D1, a promising PI3K inhibitor for prostate cancer therapy. LNCaP-specific KYL peptide was used as the targeting ligand and the prostate specific antigen (PSA) cleavable peptide (SSKYQSL) was used as the enzyme-responsive linker. SSKYQSL is cleaved by recombinant human PSA at 10-250 μg/mL. By contrast, the linker is stable in the serum of prostate cancer patients with high PSA levels (>500 ng/mL), indicating that this linker can survive the systemic circulation in prostate cancer patients but be cleaved in the tumor microenvironment. Cellular uptake of the peptide drug conjugate in prostate cancer cells is improved by about nine times. Biodistribution study reveals significant tumor accumulation of the peptide drug conjugate in nude mice bearing C4-2 tumor xenografts. Meanwhile, distribution of the conjugate in other major tissues is the same as the parent drug, indicating a high specificity of the conjugate to prostate cancers in vivo.
authors.affiliation: Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, Kansas City, MO.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Barve
authors.affiliation: Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, Kansas City, MO.
authors.forename: Akshay
authors.initials: A
authors.lastname: Jain
authors.affiliation: Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, Kansas City, MO.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, Kansas City, MO.
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.affiliation: Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, Kansas City, MO. Electronic address: chengkun@umkc.edu.
authors.forename: Kun
authors.initials: K
authors.lastname: Cheng
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D019869:Phosphatidylinositol 3-Kinases; D011471:Prostatic Neoplasms / Q000188:drug therapy*; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
chemical_list: D004791:Enzyme Inhibitors; D019869:Phosphatidylinositol 3-Kinases
keywords: C4-2; LNCaP; PSA; Patient serum; Peptide drug conjugate; Prostate cancer