title: Hydrogen sulfide improves colonic barrier integrity in DSS-induced inflammation in Caco-2 cells and mice.
abstract: Intestinal barrier involves in the pathogeny of inflammatory bowel disease (IBD) and hydrogen sulfide (H2S) has been reported to improve intestinal barrier integrity. Thus, this study investigated the effects of GYY4137, a slow-release H2S donor, on DSS-induced inflammation and intestinal dysfunction. In vitro model, cellular permeability was significantly increased and expression of tight junctions (ZO-1, Cauldin4, and Occludin) was downregulated in Caco-2 cells. GYY4137 treatment markedly attenuated DSS-induced inflammation and barrier dysfunction. Cystathionine β-synthase (CBS)-siRNA transfection further demonstrated that endogenous H2S system involves in DSS-induced inflammation and mediates barrier function. In vivo model, DSS exposure caused colonic inflammation and injury in mice and GYY4137 injection alleviated inflammatory response and improved intestinal barrier via reducing intestinal permeability and upregulating of tight junctions. In conclusion, endogenous H2S system involves in DSS-induced inflammation and H2S addition alleviated inflammation and intestinal dysfunction in vitro and in vivo.
authors.affiliation: Department of Anesthesiology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yan
authors.affiliation: Department of General Surgery, People's Hospital of Changji Hui Autonomous Prefecture, Changji, Xinjiang 831100, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Anesthesiology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ji
authors.affiliation: Department of Anesthesiology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, China. Electronic address: zhangnich2015@sina.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability / Q000187:drug effects; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D003541:Cystathionine beta-Synthase / Q000235:genetics / Q000378:metabolism*; D016264:Dextran Sulfate; D005260:Female; D006801:Humans; D006862:Hydrogen Sulfide / Q000627:therapeutic use*; D051379:Mice; D008815:Mice, Inbred Strains; D009025:Morpholines / Q000627:therapeutic use*; D009946:Organothiophosphorus Compounds / Q000627:therapeutic use*; D034741:RNA, Small Interfering / Q000235:genetics; D019108:Tight Junctions / Q000187:drug effects* / Q000378:metabolism
chemical_list: C529376:GYY 4137; D009025:Morpholines; D009946:Organothiophosphorus Compounds; D034741:RNA, Small Interfering; D016264:Dextran Sulfate; D003541:Cystathionine beta-Synthase; D006862:Hydrogen Sulfide
keywords: Barrier integrity; H(2)S; Inflammation