title: Characterization of Ciprofloxacin-Resistant and Ciprofloxacin-Susceptible Uropathogenic Escherichia coli Obtained from Patients with Gynecological Cancer.
abstract: The objective of this work was to assess the genetic characteristics of uropathogenic Escherichia coli, ciprofloxacin resistance or susceptibility, obtained from patients with gynecological cancer and urinary tract infection (UTI). Seventy-seven E. coli ciprofloxacin-resistant isolates and 38 ciprofloxacin-susceptible were analyzed by polymerase chain reaction (PCR) to determine the phylogenetic groups, virulence factors as iucC, fyuA, hlyC, cnf1 genes, and pks pathogenicity island. The presence of genes related to ciprofloxacin resistance such as qnrA, qnrB, qnrS, aac(6')-Ib-cr, and qepA, and the sequencing of DNA gyrase genes and topoisomerase IV were determined. The genetic profile of the isolates was determined by pulsed-field gel electrophoresis (PFGE). Statistical analysis was performed using Fisher's exact test and Chi-square test. Phylogenetic group B2 was the most prevalent although a great genetic diversity was observed by PFGE. Only genes associated to siderophores were found in ciprofloxacin-resistant isolates; however, in ciprofloxacin-susceptible isolates, genes related to siderophores and toxin, were detected. Additionally qnrB was detected in both populations, ciprofloxacin resistant and susceptible. DNA mutations in gyrA were Ser-83-Leu and Asp-87-Asn and in parC were Ser-80-Ile and Glu-84-Val, Glu-84-Lys. In conclusion, it was observed a high prevalence of qnrB in the population studied; in addition, it was the first time the pks island was observed only in ciprofloxacin-susceptible isolates.
authors.affiliation: Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
authors.forename: Muniqui S
authors.initials: MS
authors.lastname: Capett
authors.affiliation: Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.|Institut de Recherche en Santé Digestive, INSERM UMR1220, Toulouse, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Vollú-Silva
authors.affiliation: Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
authors.forename: Vanessa A
authors.initials: VA
authors.lastname: Melchiades
authors.affiliation: Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
authors.forename: Luciana C
authors.initials: LC
authors.lastname: Bokehi
authors.affiliation: Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Araújo
authors.affiliation: Faculdade de Medicina, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Ianick Souto
authors.initials: IS
authors.lastname: Martins
authors.affiliation: Instituto Biomédico, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Felipe P G
authors.initials: FPG
authors.lastname: Neves
authors.affiliation: Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
authors.forename: Alice G M
authors.initials: AGM
authors.lastname: Gonzalez
authors.affiliation: Institut de Recherche en Santé Digestive, INSERM UMR1220, Toulouse, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Oswald
authors.affiliation: Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
authors.forename: Geraldo R
authors.initials: GR
authors.lastname: de Paula
authors.affiliation: Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil. tlenise@hotmail.com.
authors.forename: Lenise A
authors.initials: LA
authors.lastname: Teixeira
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005260:Female; D005833:Genital Neoplasms, Female / Q000150:complications*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D014552:Urinary Tract Infections / Q000209:etiology / Q000382:microbiology*; D056689:Uropathogenic Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D002939:Ciprofloxacin