title: Controlled release of recombinant human cementum protein 1 from electrospun multiphasic scaffold for cementum regeneration.
abstract: Periodontitis is a major cause for tooth loss, which affects about 15% of the adult population. Cementum regeneration has been the crux of constructing the periodontal complex. Cementum protein 1 (CEMP1) is a cementum-specific protein that can induce cementogenic differentiation. In this study, poly(ethylene glycol) (PEG)-stabilized amorphous calcium phosphate (ACP) nanoparticles were prepared by wet-chemical method and then loaded with recombinant human CEMP1 (rhCEMP1) for controlled release. An electrospun multiphasic scaffold constituted of poly(ε-caprolactone) (PCL), type I collagen (COL), and rhCEMP1/ACP was fabricated. The effects of rhCEMP1/ACP/PCL/COL scaffold on the attachment proliferation, osteogenic, and cementogenic differentiations of human periodontal ligament cells, (PDLCs) were systematically investigated. A critical size defect rat model was introduced to evaluate the effect of tissue regeneration of the scaffolds in vivo. The results showed that PEG-stabilized ACP nanoparticles formed a core-shell structure with sustained release of rhCEMP1 for up to 4 weeks. rhCEMP1/ACP/PCL/COL scaffold could suppress PDLCs proliferation behavior and upregulate the expression of cementoblastic markers including CEMP1 and cementum attachment protein while downregulating osteoblastic markers including osteocalcin and osteopontin when it was cocultured with PDLCs in vitro for 7 days. Histology analysis of cementum after being implanted with the scaffold in rats for 8 weeks showed that there was cementum-like tissue formation but little bone formation. These results indicated the potential of using electrospun multiphasic scaffolds for controlled release of rhCEMP1 for promoting cementum regeneration in reconstruction of the periodontal complex.
authors.affiliation: Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Leiying
authors.initials: L
authors.lastname: Miao
authors.affiliation: Department of Materials Science and Engineering, College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Ren
authors.affiliation: Biomaterials and Tissue Engineering Group, Leeds Dental Institute, University of Leeds, Leeds, UK.
authors.forename: Xuebin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Materials Engineering, National Laboratory of Solid State Micro Structure, College of Engineering and Applied Sciences, Nanjing University, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Weibin
authors.initials: W
authors.lastname: Sun
mesh_terms: D000293:Adolescent; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects / Q000235:genetics; D002130:Calcium Phosphates / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D025021:Cementogenesis / Q000187:drug effects; D024042:Collagen Type I / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000494:pharmacology; D003739:Dental Cementum / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects; D011091:Polyesters / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology*; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology*; D012038:Regeneration / Q000187:drug effects*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C510886:CEMP1 protein, human; D002130:Calcium Phosphates; D024042:Collagen Type I; D003692:Delayed-Action Preparations; D011091:Polyesters; D011506:Proteins; D011994:Recombinant Proteins; C519480:amorphous calcium phosphate; C016240:polycaprolactone
keywords: cementum regeneration; controlled release; in vivo; nanofiber scaffold; rhCEMP1