title: Luminescent platforms for monitoring changes in the solubility of amylin and huntingtin in living cells.
abstract: Luminescent, cell-based assays capable of monitoring the aggregation potential of the disease-relevant proteins known as amylin (type 2 diabetes) and huntingtin (Huntington's disease) are presented. These sensitive platforms report on the influence of mutations as well as small molecules on protein solubility.
authors.affiliation: Department of Chemistry, University of Nebraska - Lincoln, Lincoln, NE 68588, USA. cstains2@unl.edu.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhao
authors.forename: Quyen
authors.initials: Q
authors.lastname: Vu
authors.forename: Cliff I
authors.initials: CI
authors.lastname: Stains
mesh_terms: D001419:Bacteria / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D000071058:Huntingtin Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006816:Huntington Disease / Q000378:metabolism; D058228:Islet Amyloid Polypeptide / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008163:Luminescent Measurements; D066263:Protein Aggregation, Pathological; D012995:Solubility; D013194:Staining and Labeling
chemical_list: D000071058:Huntingtin Protein; D058228:Islet Amyloid Polypeptide