title: Studying T Cells N-Glycosylation by Imaging Flow Cytometry.
abstract: Imaging flow cytometry is an emerging imaging technology that combines features of both conventional flow cytometry and fluorescence microscopy allowing quantification of the imaging parameters. The analysis of protein posttranslational modifications by glycosylation using imaging flow cytometry constitutes an important bioimaging tool in the glycobiology field. This technique allows quantification of the glycan fluorescence intensity, co-localization with proteins, and evaluation of the membrane/cytoplasmic expression. In this chapter we provide the guidelines to analyze glycan expression, particularly the β1,6 GlcNAc branched N-glycans, on the membrane of intestinal T cells from inflammatory bowel disease patients.
authors.affiliation: Institute for Research and Innovation in Health, Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Rua Júlio Amaral de Carvalho 45, 4200-135, Porto, Portugal.|Institute of Biomedical Sciences of Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Dias
authors.affiliation: Institute for Research and Innovation in Health, Instituto de Engenharia Biomédica (INEB), University of Porto, Porto, Portugal.
authors.forename: Catarina R
authors.initials: CR
authors.lastname: Almeida
authors.affiliation: Institute for Research and Innovation in Health, Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Rua Júlio Amaral de Carvalho 45, 4200-135, Porto, Portugal.|Institute of Biomedical Sciences of Abel Salazar (ICBAS), University of Porto, Porto, Portugal.|Medical Faculty, University of Porto, Porto, Portugal.
authors.forename: Celso A
authors.initials: CA
authors.lastname: Reis
authors.affiliation: Institute for Research and Innovation in Health, Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Rua Júlio Amaral de Carvalho 45, 4200-135, Porto, Portugal. salomep@ipatimup.pt.|Institute of Biomedical Sciences of Abel Salazar (ICBAS), University of Porto, Porto, Portugal. salomep@ipatimup.pt.
authors.forename: Salomé S
authors.initials: SS
authors.lastname: Pinho
mesh_terms: D003093:Colitis, Ulcerative / Q000276:immunology / Q000473:pathology*; D005434:Flow Cytometry / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D019044:Image Cytometry; D007422:Intestines / Q000276:immunology / Q000473:pathology; D008856:Microscopy, Fluorescence / Q000379:methods*; D037121:Plant Lectins / Q000276:immunology; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism* / Q000473:pathology*
chemical_list: D037121:Plant Lectins; D011134:Polysaccharides
keywords: Imaging flow cytometry; N-Glycosylation; T-Cell Receptor; T-cells; Ulcerative colitis