title: Application of SPR Imaging Biosensor for the Measurement of 20S Proteasomes in Blood Plasma of Children with Thermal Injury.
abstract: BACKGROUND
  Determination of proteasome concentration in blood plasma in children with burns, with the Surface Plasmon Resonance Imaging biosensor.
  
  
  METHODS
  35 children scalded by hot water, with burns in 4-20% TBSA were included into the study (age 9 months up to 14 years, mean age 2,5+1 years). Blood plasma 20S proteasome concentration was assessed in 2-6h, 12-16h, 3d, 5d, and 7d after injury using Surface Plasmon Resonance Imaging biosensor.
  
  
  RESULTS
  Statistically significant elevation of circulating 20S proteasome concentration was noted in all groups 12-16 hours after the injury; however, differences were more evident in children with the more severe burns. Circulating 20S proteasome concentration in the plasma was increased until day 7 in the group with severe burns, until day 5 in the group with moderate burns and until day 3 in the group with minor burns. Circulating 20S proteasome concentration in the plasma was highly correlated with burn severity.
  
  
  CONCLUSIONS
  The SPR imaging biosensor can be used for determination of 20S proteasome in plasma of patients with burns. Circulating 20S proteasomes are elevated after burn injury and slowly reach the normal range during the wound healing process. Increase in circulating 20S proteasome concentration is proportional to the severity of the burn injury and do not correlate with age or sex.
authors.affiliation: Department of Pediatric Surgery Medical University of Bialystok, University of Bialystok, Bialystok, Poland ewamat@tlen.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Matuszczak
authors.affiliation: Department of Biophysics Medical University of Bialystok, University of Bialystok, Bialystok, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Tylicka
authors.affiliation: Department of Pediatric Surgery Medical University of Bialystok, University of Bialystok, Bialystok, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Hermanowicz
authors.affiliation: Department of Pediatric Surgery Medical University of Bialystok, University of Bialystok, Bialystok, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Debek
authors.affiliation: Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Bialystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sankiewicz
authors.affiliation: Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Bialystok, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Gorodkiewicz
mesh_terms: D000293:Adolescent; D002056:Burns / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D046988:Proteasome Endopeptidase Complex / Q000097:blood*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D046988:Proteasome Endopeptidase Complex
keywords: Surface Plasmon Resonance Imaging; burns; children; circulating 20S proteasome; plasma; proteasomes