title: Paper analytical devices for dynamic evaluation of cell surface N-glycan expression via a bimodal biosensor based on multibranched hybridization chain reaction amplification.
abstract: A novel colorimetric/fluorescence bimodal lab-on-paper cyto-device was fabricated based on concanavalin A (Con A)-integrating multibranched hybridization chain reaction (mHCR). The product of mHCR was modified PtCu nanochains (colorimetric signal label) and graphene quantum dot (fluorescence signal label) for in situ and dynamically evaluating cell surface N-glycan expression. In this strategy, preliminary detection was carried out through colorimetric method, if needed, then the fluorescence method was applied for a precise determination. Au-Ag-paper devices increased the surface areas and active sites for immobilizing larger amount of aptamers, and then specifically and efficiently captured more cancer cells. Moreover, it could effectively reduce the paper background fluorescence. Due to the specific recognition of Con A with mannose and the effective signal amplification of mHCR, the proposed strategy exhibited excellent high sensitivity for the cytosensing of MCF-7 cells ranging from 100 to 1.0×10(7) and 80-5.0×10(7) cellsmL(-1) with the detection limit of 33 and 26 cellsmL(-1) for colorimetric and fluorescence, respectively. More importantly, this strategy was successfully applied to dynamically monitor cell-surface multi-glycans expression on living cells under external stimuli of inhibitors as well as for N-glycan expression inhibitor screening. These results implied that this biosensor has potential in studying complex native glycan-related biological processes and elucidating the N-glycan-related diseases in biological and physiological processes.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022 China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Liang
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022 China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Lan
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022 China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022 China; Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan,, Jinan, 250022 China. Electronic address: chm_gesg@163.com.
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022 China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, 250022 China.
authors.forename: Na
authors.initials: N
authors.lastname: Ren
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022 China.
authors.forename: Haiyun
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022 China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics; D015374:Biosensing Techniques / Q000295:instrumentation; D002462:Cell Membrane / Q000378:metabolism*; D003124:Colorimetry / Q000295:instrumentation*; D004209:Disposable Equipment; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D017403:In Situ Hybridization / Q000295:instrumentation*; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation; D010209:Paper; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D011134:Polysaccharides / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D011952:Receptors, Concanavalin A / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
chemical_list: D052157:Aptamers, Nucleotide; D011134:Polysaccharides; D011952:Receptors, Concanavalin A
keywords: Au-Ag-paper devices; Colorimetric/fluorescence bimodal biosensor; Graphene quantum dot; Multibranched hybridization chain reaction; PtCu nanochains