title: New evidences on efficacy of boronic acid-based derivatization method to identify sugars in plant material by gas chromatography-mass spectrometry.
abstract: This work presents an analytical procedure based on gas chromatography-mass spectrometry which allows the determination of aldoses (glucose, mannose, galactose, arabinose, xylose, fucose, rhamnose) and chetoses (fructose) in plant material. One peak for each target carbohydrate was obtained by using an efficient derivatization employing methylboronic acid and acetic anhydride sequentially, whereas the baseline separation of the analytes was accomplished using an ionic liquid capillary column. First, the proposed method was optimized and validated. Successively, it was applied to identify the carbohydrates present in plant material. Finally, the procedure was successfully applied to samples from a XVII century painting, thus highlighting the occurrence of starch glue and fruit tree gum as polysaccharide materials.
authors.affiliation: Laboratorio di Spettrometria di Massa Analitica ed Isotopica, Dipartimento di Beni Culturali, Università del Salento, Lecce, Italy. Electronic address: marianna.faraco@unisalento.it.
authors.forename: Marianna
authors.initials: M
authors.lastname: Faraco
authors.affiliation: Laboratorio di Spettrometria di Massa Analitica ed Isotopica, Dipartimento di Beni Culturali, Università del Salento, Lecce, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fico
authors.affiliation: Laboratorio di Spettrometria di Massa Analitica ed Isotopica, Dipartimento di Beni Culturali, Università del Salento, Lecce, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Pennetta
authors.affiliation: Laboratorio di Spettrometria di Massa Analitica ed Isotopica, Dipartimento di Beni Culturali, Università del Salento, Lecce, Italy.
authors.forename: Giuseppe E
authors.initials: GE
authors.lastname: De Benedetto
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D002241:Carbohydrates; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D009005:Monosaccharides; D010944:Plants / Q000737:chemistry*; D000073893:Sugars / Q000032:analysis*
chemical_list: D001897:Boronic Acids; D002241:Carbohydrates; D009005:Monosaccharides; D000073893:Sugars
keywords: Carbohydrate; Gas chromatography–mass spectrometry; Methylboronic acid; Plant material