title: Paenibacillus methanolicus sp. nov., a xylanolytic, methanol-utilizing bacterium isolated from the phyllosphere of bamboo (Pseudosasa japonica).
abstract: Strain BL24T, isolated from bamboo phyllosphere collected in Coimbatore, India, was studied for taxonomic classification. Cells of the strain were aerobic, Gram-stain-positive, motile, catalase- and oxidase-positive rods and grew on media containing methanol. In 16S rRNA gene sequence analysis, strain BL24Tshowed the highest sequence similarities with Paenibacillus phyllosphaeraeKACC 11473T (97.8 %) and Paenibacillus sacheonensisSY01 (95.1 %). DNA-DNA hybridization with P. phyllosphaerae KACC 11473T, phylogenetically the most closely related species, was 21.6 %; this value showed that strain BL24Tbelonged to a different species. The cell-wall peptidoglycan was found to possess meso-diaminopimelic acid and the G+C content of genomic DNA was 52.1 mol %. It contained menaquinone (MK)-7 as the predominant respiratory quinone and the major cellular fatty acids are C16 : 0, anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0. Based on the molecular and chemotaxonomic markers and physiological properties, strain BL24T (=NRRL B-51698T=CCM 7577T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillusmethanolicusis proposed.
authors.affiliation: Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore 641003, Tamilnadu, India.|Temasek Lifesciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore.
authors.forename: Munusamy
authors.initials: M
authors.lastname: Madhaiyan
authors.affiliation: Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore 641003, Tamilnadu, India.|Temasek Lifesciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore.
authors.forename: Selvaraj
authors.initials: S
authors.lastname: Poonguzhali
authors.affiliation: Department of Microbiology, Indira Gandhi College of Arts and Science, Pondicherry - 605009, India.
authors.forename: Venkatakrishnan Sivaraj
authors.initials: VS
authors.lastname: Saravanan
authors.affiliation: School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, New Zealand.
authors.forename: Dhandapani
authors.initials: D
authors.lastname: Pragatheswari
authors.affiliation: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Veeramuthu
authors.initials: V
authors.lastname: Duraipandiyan
authors.affiliation: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Naif Abdullah
authors.initials: NA
authors.lastname: Al-Dhabi
authors.affiliation: Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore 641003, Tamilnadu, India.
authors.forename: Palani
authors.initials: P
authors.lastname: Santhanakrishnan
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D007194:India; D000432:Methanol; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology*; D006109:Poaceae / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7; D000432:Methanol