title: Effect of resistance exercise intensity on the expression of PGC-1α isoforms and the anabolic and catabolic signaling mediators, IGF-1 and myostatin, in human skeletal muscle.
abstract: The purpose of this study was to investigate the acute messenger (mRNA) expression of the peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) isoforms, insulin-like growth factor-1Ea (IGF-1Ea), and myostatin in response to 2 resistance exercise intensities. In a uniform-balanced, crossover design, 10 participants performed 2 separate testing sessions involving a lower body resistance exercise component consisting of a lower intensity (50% of 1-repetition maximum; 1RM) protocol and a higher intensity (80% of 1RM) protocol of equal volumes. Muscle samples were obtained at before exercise, 45 min, 3 h, 24 h, and 48 h postexercise. Resistance exercise did not alter total PGC-1α mRNA expression; however, distinct responses of each PGC-1α isoform were observed. The response of each isoform was consistent between sessions, suggesting no effect of resistance exercise intensity on the complex transcriptional expression of the PGC-1α gene. IGF-1Ea mRNA expression significantly increased following the higher intensity session compared with pre-exercise and the lower intensity session. Myostatin mRNA expression was significantly reduced compared with pre-exercise values at all time points with no difference between exercise intensity. Further research is needed to determine the effects of the various isoforms of PGC-1α in human skeletal muscle on the translational level as well as their relation to the expression of IGF-1 and myostatin.
authors.affiliation: Exercise and Biochemical Nutrition Laboratory, Department of Health, Human Performance, and Recreation, One Bear Place #97313, Baylor University, Waco, TX 76798-7313, USA.|Exercise and Biochemical Nutrition Laboratory, Department of Health, Human Performance, and Recreation, One Bear Place #97313, Baylor University, Waco, TX 76798-7313, USA.
authors.forename: Neil A
authors.initials: NA
authors.lastname: Schwarz
authors.affiliation: Exercise and Biochemical Nutrition Laboratory, Department of Health, Human Performance, and Recreation, One Bear Place #97313, Baylor University, Waco, TX 76798-7313, USA.|Exercise and Biochemical Nutrition Laboratory, Department of Health, Human Performance, and Recreation, One Bear Place #97313, Baylor University, Waco, TX 76798-7313, USA.
authors.forename: Sarah K
authors.initials: SK
authors.lastname: McKinley-Barnard
authors.affiliation: Exercise and Biochemical Nutrition Laboratory, Department of Health, Human Performance, and Recreation, One Bear Place #97313, Baylor University, Waco, TX 76798-7313, USA.|Exercise and Biochemical Nutrition Laboratory, Department of Health, Human Performance, and Recreation, One Bear Place #97313, Baylor University, Waco, TX 76798-7313, USA.
authors.forename: Mike B
authors.initials: MB
authors.lastname: Spillane
authors.affiliation: Exercise and Biochemical Nutrition Laboratory, Department of Health, Human Performance, and Recreation, One Bear Place #97313, Baylor University, Waco, TX 76798-7313, USA.|Exercise and Biochemical Nutrition Laboratory, Department of Health, Human Performance, and Recreation, One Bear Place #97313, Baylor University, Waco, TX 76798-7313, USA.
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Andre
authors.affiliation: Exercise and Biochemical Nutrition Laboratory, Department of Health, Human Performance, and Recreation, One Bear Place #97313, Baylor University, Waco, TX 76798-7313, USA.|Exercise and Biochemical Nutrition Laboratory, Department of Health, Human Performance, and Recreation, One Bear Place #97313, Baylor University, Waco, TX 76798-7313, USA.
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Gann
authors.affiliation: Exercise and Biochemical Nutrition Laboratory, Department of Health, Human Performance, and Recreation, One Bear Place #97313, Baylor University, Waco, TX 76798-7313, USA.|Exercise and Biochemical Nutrition Laboratory, Department of Health, Human Performance, and Recreation, One Bear Place #97313, Baylor University, Waco, TX 76798-7313, USA.
authors.forename: Darryn S
authors.initials: DS
authors.lastname: Willoughby
mesh_terms: D000293:Adolescent; D000328:Adult; D015992:Body Mass Index; D018592:Cross-Over Studies; D005786:Gene Expression Regulation; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D055435:Myostatin / Q000235:genetics / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000235:genetics / Q000378:metabolism*; D010807:Physical Endurance; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055070:Resistance Training; D015398:Signal Transduction; D055815:Young Adult
chemical_list: C583557:IGF1 protein, human; D055435:Myostatin; D047495:PPAR gamma; C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I
keywords: IGF-1; PGC-1α; exercice contre résistance; exercise intensity; expression génique; gene expression; intensité de l’exercice; muscle squelettique; myostatin; myostatine; resistance exercise; skeletal muscle