title: Genome-Wide QTL Mapping for Wheat Processing Quality Parameters in a Gaocheng 8901/Zhoumai 16 Recombinant Inbred Line Population.
abstract: Dough rheological and starch pasting properties play an important role in determining processing quality in bread wheat (Triticum aestivum L.). In the present study, a recombinant inbred line (RIL) population derived from a Gaocheng 8901/Zhoumai 16 cross grown in three environments was used to identify quantitative trait loci (QTLs) for dough rheological and starch pasting properties evaluated by Mixograph, Rapid Visco-Analyzer (RVA), and Mixolab parameters using the wheat 90 and 660 K single nucleotide polymorphism (SNP) chip assays. A high-density linkage map constructed with 46,961 polymorphic SNP markers from the wheat 90 and 660 K SNP assays spanned a total length of 4121 cM, with an average chromosome length of 196.2 cM and marker density of 0.09 cM/marker; 6596 new SNP markers were anchored to the bread wheat linkage map, with 1046 and 5550 markers from the 90 and 660 K SNP assays, respectively. Composite interval mapping identified 119 additive QTLs on 20 chromosomes except 4D; among them, 15 accounted for more than 10% of the phenotypic variation across two or three environments. Twelve QTLs for Mixograph parameters, 17 for RVA parameters and 55 for Mixolab parameters were new. Eleven QTL clusters were identified. The closely linked SNP markers can be used in marker-assisted wheat breeding in combination with the Kompetitive Allele Specific PCR (KASP) technique for improvement of processing quality in bread wheat.
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural SciencesBeijing, China; Department of Plant Genetics & Breeding/State Key Laboratory for Agrobiotechnology, China Agricultural UniversityBeijing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Weie
authors.initials: W
authors.lastname: Wen
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Liu
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Zhai
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Wheat and Maize Research Center, Institute of Cotton Research, Chinese Academy of Agricultural Sciences Anyang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Plant Genetics & Breeding/State Key Laboratory for Agrobiotechnology, China Agricultural University Beijing, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Xianchun
authors.initials: X
authors.lastname: Xia
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural SciencesBeijing, China; International Maize and Wheat Improvement Center (CIMMYT) China officeBeijing, China.
authors.forename: Zhonghu
authors.initials: Z
authors.lastname: He
keywords: Mixograph; Mixolab; QTL mapping; Triticum aestivum; high-density linkage map; quality parameters; rapid visco-analyzer; single nucleotide polymorphism