title: Analysis of Ambient Influences Affecting Interleukin-6 Secretion in the Context of Clinical Trials of Stem Cell Therapy for Myocardial Infarction.
abstract: BACKGROUND
  Contradictory results of large clinical trials of stem cell therapy in acute myocardial infarction (AMI) have impeded a wider clinical use. As signalling via paracrine factors in AMI has received more and more attention recently, we sought to compare processing protocols with special emphasis on interleukin-6 (IL-6), a factor of major relevance for triggering cardioprotective signals via STAT3 and PI3K.
  
  
  METHODS
  Bone marrow and peripheral blood mononuclear cells were processed according to protocols used in the REPAIR-AMI and ASTAMI study.
  
  
  RESULTS
  Keeping cells at higher temperatures significantly boosted secretion of IL-6. Moreover, the use of autologous serum and X-Vivo medium was superior over reagents used in the protocol of the ASTAMI study.
  
  
  CONCLUSIONS
  External influencing factors (higher temperature, use of a modern cell culture medium supplemented with serum) led to higher concentrations of IL-6. These results could provide an explanation for the superior results found in the REPAIR-AMI study.
authors.forename: Christoph
authors.initials: C
authors.lastname: Edlinger
authors.forename: Bernhard
authors.initials: B
authors.lastname: Wernly
authors.forename: Michael
authors.initials: M
authors.lastname: Leisch
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kammler
authors.forename: Alexander
authors.initials: A
authors.lastname: Kypta
authors.forename: Sarah
authors.initials: S
authors.lastname: Eder
authors.forename: Christian
authors.initials: C
authors.lastname: Jung
authors.forename: Uta C
authors.initials: UC
authors.lastname: Hoppe
authors.forename: Michael
authors.initials: M
authors.lastname: Lichtenauer
mesh_terms: D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002986:Clinical Trials as Topic / Q000379:methods*; D003470:Culture Media / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery*; D012107:Research Design; D015398:Signal Transduction; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000378:metabolism*; D013696:Temperature; D016896:Treatment Outcome
chemical_list: D003470:Culture Media; C508600:IL6 protein, human; D015850:Interleukin-6