title: miR-34C Disrupts the Stemness of Purified CD133+Prostatic Cancer Stem Cells.
abstract: OBJECTIVE
  To find the potential micro-RNA (miRNA) that could determine the fate of prostate cancer stem cells.
  
  
  METHODS
  We compared miRNA expression between our purified CD133+prostatic cancer stem cells (PCSCs) and CD133-cells. Sphere formation assay and matrigel-based cell invasion assay were applied to determine the stemness of CD133+PCSCs after our manipulation of miRNA using miRNA mimic or miRNA inhibitor.
  
  
  RESULTS
  In this study, we identified that miR-34C was under-expressed in the purified CD133+PCSCs and enforced introduction of miR-34C attenuated the stemness of CD133+PCSCs. Clinically, we also observed a negative correlation between miR-34C and CD133.
  
  
  CONCLUSIONS
  Our data strongly suggest that miR-34C may play essential role in conferring castration resistance by equilibrating PSCS population.
authors.affiliation: Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Gynaecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Rao
authors.affiliation: Center of Reproductive Medicine, Institute of Family Planning Research, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Cuntai
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Qianyuan
authors.initials: Q
authors.lastname: Zhuang
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: qzhangdoc@126.com.
authors.forename: Zhangqun
authors.initials: Z
authors.lastname: Ye
mesh_terms: D000071916:AC133 Antigen / Q000096:biosynthesis; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D014411:Neoplastic Stem Cells / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D000071916:AC133 Antigen; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; C000606023:PROM1 protein, human