title: Epigenetic changes in cancer by Raman imaging, fluorescence imaging, AFM and scanning near-field optical microscopy (SNOM). Acetylation in normal and human cancer breast cells MCF10A, MCF7 and MDA-MB-231.
abstract: This paper examines epigenetic changes in breast cancer by Raman imaging, fluorescence imaging, AFM and SNOM and discusses how they contribute to different aspects of tumourigenesis in malignant human breast epithelial cell lines MCF7 and MDA-MB-231 compared with non-malignant MCF10A cell lines. The paper focuses on information that can be extracted from Raman microscopy and Raman imaging for the biological material of nucleoli contained within the cell nucleus and lipid droplets within the cell cytoplasm. The biochemical composition of the nuclei and lipid droplets in the non-malignant and malignant human breast epithelial cell lines has been monitored. The potential of Raman microspectroscopy to monitor acetylation processes and a prognostic value of Raman biomarkers in breast cancer have been discussed.
authors.affiliation: Institute of Applied Radiation Chemistry, Laboratory of Laser Molecular Spectroscopy, Lodz University of Technology, Lodz, Poland. abramczy@mitr.p.lodz.pl.
authors.forename: Halina
authors.initials: H
authors.lastname: Abramczyk
authors.forename: Jakub
authors.initials: J
authors.lastname: Surmacki
authors.forename: Monika
authors.initials: M
authors.lastname: Kopeć
authors.forename: Alicja Klaudia
authors.initials: AK
authors.lastname: Olejnik
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kaufman-Szymczyk
authors.forename: Krystyna
authors.initials: K
authors.lastname: Fabianowska-Majewska
mesh_terms: D000107:Acetylation; D014408:Biomarkers, Tumor / Q000032:analysis*; D001940:Breast / Q000000981:diagnostic imaging*; D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000235:genetics*; D045744:Cell Line, Tumor; D044127:Epigenesis, Genetic; D006801:Humans; D008853:Microscopy; D018625:Microscopy, Atomic Force; D061848:Optical Imaging; D013059:Spectrum Analysis, Raman
chemical_list: D014408:Biomarkers, Tumor