title: [Expression profiles of long non-coding RNAs in human liver cell line LO2 with stable expression of hepatitis B x gene].
abstract: OBJECTIVE
  To investigate the differential expression profiles of long non-coding RNAs (lncRNAs) in human liver cell line LO2 with stable expression of hepatitis B x (HBx) gene, and to screen out the lncRNAs which play an important role in HBV-related liver cancer.
  
  
  METHODS
  The lncRNA microarray was used to establish the differential expression profiles of lncRNAs, and the methods such as scatter plots and cluster analysis were used to obtain the HBx-related lncRNAs with differential expression. The qRT-PCR was used to verify some lncRNAs with differential expression. The t-test was used to compare the expression of lncRNAs between the two microarray groups, and hierarchical cluster analysis was used for the original data of lncRNAs with differential expression.
  
  
  RESULTS
  Compared with the control group transfected with blank plasmids (L02/pcDNA3.0), LO2/HBx cells had 323 lncRNAs with > 2-fold upregulation and 421 lncRNAs whose expression was reduced by more than 50% (P < 0.05). The results of qRT-PCR verified 4 upregulated lncRNAs (TCONS_00006195, ENST00000557524, NR_037597, and ENST00000539975) and 3 downregulated lncRNAs (ENST00000508424, ENST00000447433, and uc001lva.4), which were consistent with the results of microassay.
  
  
  CONCLUSIONS
  HBx-related lncRNAs are successfully screened out, which lays a foundation for further investigation of the role of lncRNAs in the pathogenesis of liver cancer.
authors.affiliation: Department of Infectious Diseases, Xiangya Hospital, Central South University, and Hunan Key Laboratory of Viral Hepatitis, Changsha 410008, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Kuang
authors.affiliation: Department of Blood Infusion, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Infectious Diseases, Xiangya Hospital, Central South University, and Hunan Key Laboratory of Viral Hepatitis, Changsha 410008, China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Fu
authors.affiliation: Department of Infectious Diseases, Xiangya Hospital, Central South University, and Hunan Key Laboratory of Viral Hepatitis, Changsha 410008, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Infectious Diseases, Xiangya Hospital, Central South University, and Hunan Key Laboratory of Viral Hepatitis, Changsha 410008, China.
authors.forename: X G
authors.initials: XG
authors.lastname: Fan
mesh_terms: D002460:Cell Line; D015536:Down-Regulation; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics*; D059467:Transcriptome; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D062085:RNA, Long Noncoding; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein