title: Involvement of syk and VEGF-C in invasion of lung adenocarcinoma A549 cells.
abstract: OBJECTIVE
  Lung cancer has become one of the most dangerous malignant tumors in the world nowadays, whose pathogenesis is complex involving multi-genes and multi-elements. This study aims to investigate the values of spleen tyrosine kinase (Syk) and vascular endothelial growth factor-C (VEGF-C) in lymphangiogenesis and metastasis of lung adenocarcinoma A549 cells.
  
  
  METHODS
  The pcDNA3.1-VEGF-C and pLNCX-syk were constructed and transfected into A549 cells. After cells with stable expression were sorted, the level of VEGF-C was tested by RT-PCR and immunohistochemistry and the mRNA of syk was tested by RT-PCR. The cell invasion assay was investigated by transwell chamber in vitro. Restriction enzyme digestion and gel electrophoresis demonstrated successful construction of the pcDNA3.1-VEGF-C.
  
  
  RESULTS
  RT-PCR and immunohistochemistry revealed higher expression of VEGF-C in VEGFC-construct-transfected A549 cells than that in controls (P < 0.05). Successful construction of the pLNCX-syk was demonstrated by restriction enzyme electrophoresis and sequencing. RT-PCR revealed Syk expression higher in syk-construct-transfected cells than in controls (P < 0.05).
  
  
  CONCLUSIONS
  The results indicate a potential link between the upregulation of Syk and VEGF-C expression and lung adenocarcinoma.
authors.affiliation: Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, Shandong, China.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, Shandong, China.
authors.forename: Chuanliang
authors.initials: C
authors.lastname: Peng
authors.affiliation: Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, Shandong, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Cong
authors.affiliation: Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, Shandong, China.
authors.forename: Yingtao
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, Shandong, China.
authors.forename: Jiazhong
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, Shandong, China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, Shandong, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D042583:Lymphangiogenesis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D000072377:Syk Kinase / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D042582:Vascular Endothelial Growth Factor C / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000502:physiology
chemical_list: D042582:Vascular Endothelial Growth Factor C; D040321:Vascular Endothelial Growth Factor Receptor-3; C000606896:SYK protein, human; D000072377:Syk Kinase