title: Highly efficient enrichment of phosphopeptides by a magnetic lanthanide metal-organic framework.
abstract: Highly efficient enrichment of phosphopeptides from complex biological samples is crucial prior to mass spectrometry analysis due to the low abundance and ion suppression effects. In this study, a facile route was designed for preparation of a magnetic erbium(Er)-based metal-organic framework (denoted as Fe3O4@PDA@Er(btc)), which was synthesized with 1,3,5-benzenetricarboxylic acid(H3btc) as ligand and grafted on the polydopamine (PDA) - coated Fe3O4. The as-prepared material exhibited ultra-high sensitivity (detection limit of 20amol/μL) and selectivity at a low mass ratio of β-Casein/BSA (1:500). Moreover, it was also investigated for enrichment of phosphopeptides from human serum, which provided a promising technique for highly efficient enrichment of low-abundance phosphorylated peptides in the practical application.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China.
authors.forename: Yiqin
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China. Electronic address: chdeng@fudan.edu.cn.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
mesh_terms: D002364:Caseins / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D057230:Limit of Detection; D008280:Magnetics; D000073396:Metal-Organic Frameworks; D010748:Phosphopeptides / Q000097:blood / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D002364:Caseins; D028581:Lanthanoid Series Elements; D000073396:Metal-Organic Frameworks; D010748:Phosphopeptides; D012710:Serum Albumin, Bovine
keywords: Lanthanide; MOF; Mass spectrometry; Phosphopeptide enrichment