title: Chorein interacts with α-tubulin and histone deacetylase 6, and overexpression preserves cell viability during nutrient deprivation in human embryonic kidney 293 cells.
abstract: The autophagy pathway has recently been implicated in several neurodegenerative diseases. Recently, it was reported that chorein-depleted cells showed accumulation of autophagic markers and impaired autophagic flux. Here, we demonstrate that chorein overexpression preserves cell viability from starvation-induced cell death in human embryonic kidney 293 (HEK293) cells. Subsequent coimmunoprecipitation and reverse coimmunoprecipitation assays using extracts from chorein that stably overexpressed HEK293 cells revealed that chorein interacts with α-tubulin and histone deacetylase 6, a known α-tubulin deacetylater and central component of basal autophagy. Indeed, acetylated α-tubulin immunoreactivity was significantly decreased in chorein that stably overexpressed HEK293 cells. These results suggest that chorein/histone deacetylase 6/α-tubulin interactions may play an important role in starvation-induced cell stress, and their disruption may be one of the molecular pathogenic mechanisms of chorea-acanthocytosis.-Sasaki, N., Nakamura, M., Kodama, A., Urata, Y., Shiokawa, N., Hayashi, T., Sano, A. Chorein interacts with α-tubulin and histone deacetylase 6, and overexpression preserves cell viability during nutrient deprivation in human embryonic kidney 293 cells.
authors.affiliation: Department of Psychiatry, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Natsuki
authors.initials: N
authors.lastname: Sasaki
authors.affiliation: Department of Psychiatry, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan nakamu36@m.kufm.kagoshima-u.ac.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nakamura
authors.affiliation: Department of Psychiatry, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kodama
authors.affiliation: Department of Psychiatry, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Urata
authors.affiliation: Department of Psychiatry, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Nari
authors.initials: N
authors.lastname: Shiokawa
authors.affiliation: Department of Psychiatry, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Department of Psychiatry, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Sano
mesh_terms: D000107:Acetylation; D000818:Animals; D001343:Autophagy / Q000502:physiology; D002470:Cell Survival; D002478:Cells, Cultured; D057809:HEK293 Cells; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D007668:Kidney / Q000196:embryology* / Q000378:metabolism*; D018345:Mice, Knockout; D014404:Tubulin / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D014404:Tubulin; D033921:Vesicular Transport Proteins; D006655:Histone Deacetylases
keywords: VPS13A; acetylated tubulin; autophagy; chorea-acanthocytosis; starvation