title: In vivo effect of copper status on cisplatin-induced nephrotoxicity.
abstract: Cisplatin is a widely used antitumor agent; however, tumor resistance and severe side effects limit its use. It is well accepted that cisplatin toxicity can be modulated in vitro in cell cultures by copper salts. In the present work, mice with different blood serum copper status were treated with a single intraperitoneal cisplatin injection at a dose of 5 mg/kg, monitored for 3 days in metabolic cages and analyzed for renal function. Both copper-deficient and copper-overloaded mice displayed more severe early proteinuria and retarded platinum excretion than control mice. The effects of copper status on cisplatin-induced nephrotoxicity are discussed.
authors.affiliation: Laboratory of Trace Elements Metabolism, ITMO University, Kronverkskiy Pr. 49, St. Petersburg, Russia, 197101.|Department of Molecular Genetics, Institute of Experimental Medicine, Pavlova Str., 12, St. Petersburg, Russia, 197376.|Biophysics Department, Institute of Physics, Nanotechnology and Telecommunications, Peter the Great St. Petersburg Polytechnic University, Politekhnicheskaya Str., 29, St. Petersburg, Russia, 195251.
authors.forename: Ludmila V
authors.initials: LV
authors.lastname: Puchkova
authors.affiliation: Biophysics Department, Institute of Physics, Nanotechnology and Telecommunications, Peter the Great St. Petersburg Polytechnic University, Politekhnicheskaya Str., 29, St. Petersburg, Russia, 195251. colbug@mail.ru.
authors.forename: Alexey N
authors.initials: AN
authors.lastname: Skvortsov
authors.affiliation: Laboratory of Molecular Pharmacology, IRCCS - Istituto di Ricerche Farmacologiche "Mario Negri", Via La Masa, 19, 20156, Milan, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Rusconi
authors.affiliation: Laboratory of Trace Elements Metabolism, ITMO University, Kronverkskiy Pr. 49, St. Petersburg, Russia, 197101.|Department of Molecular Genetics, Institute of Experimental Medicine, Pavlova Str., 12, St. Petersburg, Russia, 197376.
authors.forename: Ekaterina Yu
authors.initials: EY
authors.lastname: Ilyechova
authors.affiliation: Laboratory of Molecular Pharmacology, IRCCS - Istituto di Ricerche Farmacologiche "Mario Negri", Via La Masa, 19, 20156, Milan, Italy.|Laboratory of Trace Elements Metabolism, ITMO University, Kronverkskiy Pr. 49, St. Petersburg, Russia, 197101.
authors.forename: Massimo
authors.initials: M
authors.lastname: Broggini
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000633:toxicity*; D002945:Cisplatin / Q000008:administration & dosage / Q000633:toxicity*; D019327:Copper Sulfate / Q000008:administration & dosage / Q000097:blood* / Q000633:toxicity; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010984:Platinum / Q000032:analysis / Q000493:pharmacokinetics; D011507:Proteinuria / Q000139:chemically induced* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D000970:Antineoplastic Agents; D010984:Platinum; D019327:Copper Sulfate; D002945:Cisplatin
keywords: Cisplatin; Murine model of copper status; Nephrotoxicity