title: 'tieredScreen' - Layered Virtual Screening Tool for the Identification of Novel Estrogen Receptor Alpha Modulators.
abstract: A novel tiered Structure-Based (SB) Virtual Screening (VS) workflow called tieredScreen was designed and implemented. The automated protocol utilises diverse computational tools in a synergistic manner to reduce false positives and increase the likelihood of converging on putative active molecules. The performance of the novel VS workflow was validated using the Directory of Useful Decoys (DUD) Estrogen Receptor α (ERα) antagonist dataset, and successfully deployed for the identification of novel antagonists of ERα from a screening collection of ca. 160 000 commercially available compounds. As well as yielding nanomolar (nM) active ligands identified previously through a docking only protocol, from a selection of eight virtual hits suggested by tieredScreen, four novel nM ERα binding chemotypes were identified and biologically validated - demonstrating the applicability of a tiered intervention for virtual screening.
authors.affiliation: Molecular Design Group, School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland, Telephone: +353-1-8961634, Fax: +353-1-6762400.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Molecular Design Group, School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland, Telephone: +353-1-8961634, Fax: +353-1-6762400.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Carta
authors.affiliation: Molecular Design Group, School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland, Telephone: +353-1-8961634, Fax: +353-1-6762400.
authors.forename: Martin B
authors.initials: MB
authors.lastname: Peters
authors.affiliation: Molecular Design Group, School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland, Telephone: +353-1-8961634, Fax: +353-1-6762400.
authors.forename: Trevor
authors.initials: T
authors.lastname: Price
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin 2, Ireland.
authors.forename: Niamh
authors.initials: N
authors.lastname: O'Boyle
authors.affiliation: Molecular Design Group, School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland, Telephone: +353-1-8961634, Fax: +353-1-6762400.
authors.forename: Andrew J S
authors.initials: AJ
authors.lastname: Knox
authors.affiliation: Molecular Design Group, School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland, Telephone: +353-1-8961634, Fax: +353-1-6762400.
authors.forename: Darren
authors.initials: D
authors.lastname: Fayne
authors.affiliation: Molecular Design Group, School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland, Telephone: +353-1-8961634, Fax: +353-1-6762400.
authors.forename: D Clive
authors.initials: DC
authors.lastname: Williams
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin 2, Ireland.
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Meegan
authors.affiliation: Molecular Design Group, School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland, Telephone: +353-1-8961634, Fax: +353-1-6762400. lloyddg@tcd.ie.
authors.forename: David G
authors.initials: DG
authors.lastname: Lloyd
keywords: Docking; Drug Design; Estrogen receptor; Molecular modelling; Nuclear hormone receptor; Pharmacophore; Shape matching; Virtual screening