title: Chx10 Consolidates V2a Interneuron Identity through Two Distinct Gene Repression Modes.
abstract: During development, two cell types born from closely related progenitor pools often express identical transcriptional regulators despite their completely distinct characteristics. This phenomenon implies the need for a mechanism that operates to segregate the identities of the two cell types throughout differentiation after initial fate commitment. To understand this mechanism, we investigated the fate specification of spinal V2a interneurons, which share important developmental genes with motor neurons (MNs). We demonstrate that the paired homeodomain factor Chx10 functions as a critical determinant for V2a fate and is required to consolidate V2a identity in postmitotic neurons. Chx10 actively promotes V2a fate, downstream of the LIM-homeodomain factor Lhx3, while concomitantly suppressing the MN developmental program by preventing the MN-specific transcription complex from binding and activating MN genes. This dual activity enables Chx10 to effectively separate the V2a and MN pathways. Our study uncovers a widely applicable gene regulatory principle for segregating related cell fates.
authors.affiliation: Pediatric Neuroscience Research Program, Papé Family Pediatric Research Institute, Department of Pediatrics, Oregon Health and Science University, Portland, OR 97239, USA.
authors.forename: Yoanne M
authors.initials: YM
authors.lastname: Clovis
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Korea.
authors.forename: So Yeon
authors.initials: SY
authors.lastname: Seo
authors.affiliation: Pediatric Neuroscience Research Program, Papé Family Pediatric Research Institute, Department of Pediatrics, Oregon Health and Science University, Portland, OR 97239, USA.
authors.forename: Ji-Sun
authors.initials: JS
authors.lastname: Kwon
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Korea.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Rhee
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Korea.
authors.forename: Sujeong
authors.initials: S
authors.lastname: Yeo
authors.affiliation: Pediatric Neuroscience Research Program, Papé Family Pediatric Research Institute, Department of Pediatrics, Oregon Health and Science University, Portland, OR 97239, USA.
authors.forename: Jae W
authors.initials: JW
authors.lastname: Lee
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Korea. Electronic address: leeseung@snu.ac.kr.
authors.forename: Seunghee
authors.initials: S
authors.lastname: Lee
authors.affiliation: Pediatric Neuroscience Research Program, Papé Family Pediatric Research Institute, Department of Pediatrics, Oregon Health and Science University, Portland, OR 97239, USA; Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA. Electronic address: leesoo@ohsu.edu.
authors.forename: Soo-Kyung
authors.initials: SK
authors.lastname: Lee
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002645:Chickens; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D018398:Homeodomain Proteins / Q000378:metabolism*; D007395:Interneurons / Q000378:metabolism*; D060850:LIM-Homeodomain Proteins / Q000378:metabolism*; D009046:Motor Neurons / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation / Q000502:physiology
chemical_list: D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; D014157:Transcription Factors
keywords: Chx10; Lhx3; Sox14; V2a interneurons; Vsx2; development; motor neurons; spinal cord; transcription factor