title: Treatment with soy isoflavones during early adulthood improves metabolism in early postnatally overfed rats.
abstract: OBJECTIVE
  The incidences of obesity and related diseases have reached epidemic proportions, and new therapeutic approaches are needed. Soy isoflavones have been identified as an important dietary factor for preventing and treating metabolic dysfunction. This study examined the effects of high doses of isoflavone on glucose and fat metabolism in a model of programmed obesity and evaluated its effects on the autonomic nervous system.
  
  
  METHODS
  Litters of Wistar rats were standardized at nine pups per dam in normal litters (NL) or reduced to three pups per dam at the third day of life (P3) in small litters (SL) to induce postnatal overfeeding. Gavage with a soy bean isoflavone mixture (1 g/day) diluted in water was started at P60 and continued for 30 days. The control animals received vehicle gavage. At P90, biometric and metabolic parameters as well as direct autonomic nerve activity were measured.
  
  
  RESULTS
  Increases in glycaemia and insulinaemia observed in SL rats were reduced by isoflavone treatment, which also caused lower glucose-induced insulin secretion by pancreatic islets. Sympathetic activity in the major splanchnic nerve was increased, while vagus nerve activity was reduced by isoflavone treatment. The dyslipidaemia induced by overfeeding in SL rats was restored by isoflavone treatment.
  
  
  CONCLUSIONS
  The present study shows that treatment with isoflavone reduces adiposity and improves glucose and lipid metabolism. Collectively, these effects may depend on autonomic changes.
authors.affiliation: a Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology , State University of Maringá , PR , Brazil.
authors.forename: Pamelli
authors.initials: P
authors.lastname: Silva
authors.affiliation: a Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology , State University of Maringá , PR , Brazil.
authors.forename: Tatiane Aparecida
authors.initials: TA
authors.lastname: Ribeiro
authors.affiliation: a Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology , State University of Maringá , PR , Brazil.
authors.forename: Laize Peron
authors.initials: LP
authors.lastname: Tófolo
authors.affiliation: a Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology , State University of Maringá , PR , Brazil.
authors.forename: Kelly Valério
authors.initials: KV
authors.lastname: Prates
authors.affiliation: a Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology , State University of Maringá , PR , Brazil.
authors.forename: Flávio Andrade
authors.initials: FA
authors.lastname: Francisco
authors.affiliation: a Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology , State University of Maringá , PR , Brazil.
authors.forename: Sandra da Silva
authors.initials: SDS
authors.lastname: Silveira
authors.affiliation: a Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology , State University of Maringá , PR , Brazil.
authors.forename: Ananda
authors.initials: A
authors.lastname: Malta
authors.affiliation: a Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology , State University of Maringá , PR , Brazil.
authors.forename: Denise Alves
authors.initials: DA
authors.lastname: Lopes
authors.affiliation: a Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology , State University of Maringá , PR , Brazil.
authors.forename: Rosiane Aparecida
authors.initials: RA
authors.lastname: Miranda
authors.affiliation: a Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology , State University of Maringá , PR , Brazil.
authors.forename: Kesia
authors.initials: K
authors.lastname: Palma-Rigo
authors.affiliation: a Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology , State University of Maringá , PR , Brazil.
authors.forename: Rosana
authors.initials: R
authors.lastname: Torrezan
authors.affiliation: a Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology , State University of Maringá , PR , Brazil.
authors.forename: Paulo Cezar de Freitas
authors.initials: PCF
authors.lastname: Mathias
mesh_terms: D050154:Adiposity / Q000187:drug effects; D000818:Animals; D000831:Animals, Newborn; D001786:Blood Glucose / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood; D007529:Isoflavones / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D009765:Obesity / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism*; D044343:Overnutrition / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013025:Glycine max / Q000737:chemistry; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D007328:Insulin; D007529:Isoflavones; D014280:Triglycerides; D002784:Cholesterol
keywords: Autonomic nervous system; Glucose metabolism; Isoflavone; Lipid panel; Metabolic dysfunction; Small litters