title: Quantification of A. fumigatus-specific CD154+ T-cells-preanalytic considerations.
abstract: Fungal specific CD154+T-cells have been described as a biomarker in invasive aspergillosis. The influence of sample storage on the detection of these cells was assessed. Six-hour delay prior to PBMC isolation is associated with an 18% decrease of cell viability and alterations of the cellular composition of the sample. This results in 87% reduction of CD154+A. fumigatus specific cells due to reduced assay sensitivity and increased background values in unstimulated samples. If prompt cell measurement is not feasible, isolated PBMCs can be frozen (at -20°C and -80°C) and processed later with comparable assay reliability (mean value fresh vs. thawing: 0.126, 0.133; Pearson-Coefficient: 0.962).
authors.affiliation: University Hospital of Wuerzburg, Department of Internal Medicine II, Infectious Diseases, Oberduerr-bacher Str. 6, 97080 Wuerzburg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Wurster
authors.affiliation: University Hospital of Wuerzburg, Department of Internal Medicine II, Infectious Diseases, Oberduerr-bacher Str. 6, 97080 Wuerzburg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Weis
authors.affiliation: University Hospital of Wuerzburg, Department of Internal Medicine II, Infectious Diseases, Oberduerr-bacher Str. 6, 97080 Wuerzburg, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Page
authors.affiliation: University Hospital of Wuerzburg, Department of Internal Medicine II, Infectious Diseases, Oberduerr-bacher Str. 6, 97080 Wuerzburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Lazariotou
authors.affiliation: University Hospital of Wuerzburg, Department of Internal Medicine II, Infectious Diseases, Oberduerr-bacher Str. 6, 97080 Wuerzburg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Einsele
authors.affiliation: University Hospital of Wuerzburg, Department of Internal Medicine II, Infectious Diseases, Oberduerr-bacher Str. 6, 97080 Wuerzburg, Germany andrew.ullmann@uni-wuerzburg.de.
authors.forename: A J
authors.initials: AJ
authors.lastname: Ullmann
mesh_terms: D001232:Aspergillus fumigatus / Q000276:immunology*; D023201:CD40 Ligand / Q000032:analysis*; D002470:Cell Survival; D005260:Female; D005615:Freezing; D006801:Humans; D055744:Invasive Pulmonary Aspergillosis / Q000175:diagnosis*; D018655:Lymphocyte Count; D008297:Male; D011309:Preservation, Biological; D013048:Specimen Handling / Q000379:methods*; D016176:T-Lymphocyte Subsets / Q000737:chemistry* / Q000276:immunology*; D013696:Temperature; D013997:Time Factors; D055815:Young Adult
chemical_list: D023201:CD40 Ligand
keywords: Aspergillus fumigatus; Biomarker; Immune response; Immunology