title: Development of an Injectable Calcium Phosphate/Hyaluronic Acid Microparticles System for Platelet Lysate Sustained Delivery Aiming Bone Regeneration.
abstract: Despite the biocompatibility and osteoinductive properties of calcium phosphate (CaP) cements their low biodegradability hampers full bone regeneration. Herein the incorporation of CaP cement with hyaluronic acid (HAc) microparticles loaded with platelet lysate (PL) to improve the degradability and biological performance of the cements is proposed. Cement formulations incorporating increasing weight ratios of either empty HAc microparticles or microparticles loaded with PL (10 and 20 wt%) are developed as well as cements directly incorporating PL. The direct incorporation of PL improves the mechanical properties of the plain cement, reaching values similar to native bone. Morphological analysis shows homogeneous particle distribution and high interconnectivity between the HAc microparticles. The cements incorporating PL (with or without the HAc microparticles) present a sustained release of PL proteins for up to 8 d. The sustained release of PL modulates the expression of osteogenic markers in seeded human adipose tissue derived stem cells, thus suggesting the stimulatory role of this hybrid system toward osteogenic commitment and bone regeneration applications.
authors.affiliation: 3B's Research Group, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark Zona Industrial da Gandra, 4805-017, Barco GMR, Portugal.
authors.forename: Pedro S
authors.initials: PS
authors.lastname: Babo
authors.affiliation: 3B's Research Group, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark Zona Industrial da Gandra, 4805-017, Barco GMR, Portugal.
authors.forename: Vítor E
authors.initials: VE
authors.lastname: Santo
authors.affiliation: 3B's Research Group, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark Zona Industrial da Gandra, 4805-017, Barco GMR, Portugal.
authors.forename: Manuela E
authors.initials: ME
authors.lastname: Gomes
authors.affiliation: 3B's Research Group, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark Zona Industrial da Gandra, 4805-017, Barco GMR, Portugal.
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001792:Blood Platelets / Q000737:chemistry*; D001843:Bone Cements / Q000737:chemistry / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation; D001843:Bone Cements; D002130:Calcium Phosphates; D006820:Hyaluronic Acid; C020243:calcium phosphate
keywords: bone regeneration; drug delivery; growth factors; injectable CaP cements; platelet lysate