title: Exploring the Medicinal Potential of the Fruit Bodies of Oyster Mushroom, Pleurotus ostreatus (Agaricomycetes), against Multidrug-Resistant Bacterial Isolates.
abstract: Bacterial resistance to present-generation antibiotics is increasing drastically, which has become a major public health concern. The present study focuses on demonstrating the antimicrobial potential of fruit bodies of the culinary/medicinal oyster mushroom Pleurotus ostreatus against clinical pathogens. Five bacterial isolates were collected from Sagar Hospital in Bangalore, India. The collected strains were grown on selective and differential media and antibiotic susceptibility testing was applied using 48 antibiotics by disc diffusion assay. The antibacterial efficiency of the mushroom extract against clinical pathogens, which were found to be multidrug resistant (MDR) to most of the tested antibiotics, was studied. The yield of cultivated mushrooms was evident at moist, cooler, and humid conditions. The clinical isolates of Staphylococcus aureus, Salmonella typhi, Acinetobacter sp., Proteus mirabilis, and Proteus spp. were found to be MDR to β-lactam, fluoroquinolones, sulfonamides, third- and fourth-generation cephalosporins, aminoglycosides, macrolides, tetracyclines, and carbapenems. The methanolic extracts of mushroom fruit bodies were found to be more effective than present-generation antibiotics against methicillin- and vancomycin- resistant S. aureus, S. typhi, Acinetobacter sp., and P. mirabilis at a concentration ranging from 50 to 100 µg/disc or 50 to 100 µL/well. The current study suggests that the methanolic extract of P. ostreatus can be used as a promising antibacterial agent against MDR bacterial pathogens.
authors.affiliation: Department of Biotechnology Engineering, Dayananda Sagar Institutions, Bengaluru 560 078, Visvesvaraya Technological University, Belagavi, Karnataka, India.
authors.forename: Sinosh
authors.initials: S
authors.lastname: Skariyachan
authors.affiliation: Department of Biotechnology Engineering, Dayananda Sagar Institutions, Bengaluru 560 078, Visvesvaraya Technological University, Belagavi, Karnataka, India.
authors.forename: Apoorva
authors.initials: A
authors.lastname: Prasanna
authors.affiliation: Department of Biotechnology Engineering, Dayananda Sagar Institutions, Bengaluru 560 078, Visvesvaraya Technological University, Belagavi, Karnataka, India.
authors.forename: Sirisha P
authors.initials: SP
authors.lastname: Manjunath
authors.affiliation: Department of Biotechnology Engineering, Dayananda Sagar Institutions, Bengaluru 560 078, Visvesvaraya Technological University, Belagavi, Karnataka, India.
authors.forename: Soujanya S
authors.initials: SS
authors.lastname: Karanth
authors.affiliation: Department of Biotechnology Engineering, Dayananda Sagar Institutions, Bengaluru 560 078, Visvesvaraya Technological University, Belagavi, Karnataka, India.
authors.forename: Ambika
authors.initials: A
authors.lastname: Nazre
mesh_terms: D000150:Acinetobacter / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D045424:Complex Mixtures / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D020076:Pleurotus / Q000737:chemistry*; D011511:Proteus / Q000187:drug effects; D012485:Salmonella typhi / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D045424:Complex Mixtures