title: A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria.
abstract: Transcription activator-like effectors (TALEs) are sequence-specific DNA binding proteins found in a range of plant pathogenic bacteria, where they play important roles in host-pathogen interactions. However, it has been unclear how TALEs, after they have been injected into the host cells, activate transcription of host genes required for infection success. Here, we show that the basal transcription factor IIA gamma subunit TFIIAγ5 from rice is a key component for infection by the TALE-carrying bacterium Xanthomonas oryzae pv. oryzae, the causal agent for bacterial blight. Direct interaction of several TALEs with TFIIAγ5 is required for activation of disease susceptibility genes. Conversely, reduced expression of the TFIIAγ5 host gene limits the induction of susceptibility genes and thus decreases bacterial blight symptoms. Suppression or mutation of TFIIAγ5 can also reduce bacterial streak, another devastating disease of rice caused by TALE-carrying X. oryzae pv. oryzicola. These results have important implications for formulating a widely applicable strategy with which to improve resistance of plants to TALE-carrying pathogens.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yuan
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.
authors.forename: Yinggen
authors.initials: Y
authors.lastname: Ke
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.
authors.forename: Renyan
authors.initials: R
authors.lastname: Huang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ma
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.
authors.forename: Zeyu
authors.initials: Z
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Taian, China.
authors.forename: Zhaohui
authors.identifier: 0000-0001-8320-7872
authors.initials: Z
authors.lastname: Chu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Xiao
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.
authors.forename: Xianghua
authors.initials: X
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.
authors.forename: Shiping
authors.identifier: 0000-0002-8743-3129
authors.initials: S
authors.lastname: Wang
mesh_terms: D054884:Host-Pathogen Interactions; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D000071336:Transcription Activator-Like Effectors / Q000378:metabolism*; D035561:Transcription Factor TFIIA / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism*; D014974:Xanthomonas / Q000472:pathogenicity*
chemical_list: D000071336:Transcription Activator-Like Effectors; D035561:Transcription Factor TFIIA; D037521:Virulence Factors
keywords: Xanthomonas; plant biology; rice; transcription factor IIA