title: Detection of selected periodontal bacteria in preschool children affected by early childhood caries.
abstract: The aim of this study was to compare the detection frequency of periodontal bacteria in dental plaque in children with early childhood caries (ECC) with and without gingival inflammation. A convenience sample of 25 preschool children (mean age 3.61 years, SD 1.42) was recruited. Dental plaque was taken from periodontal areas with and without visible signs of inflammation and processed using the StomaGene® (Protean s.r.o. Czech Republic) and ParoCheck® 20 (Greiner Bio-one GmbH, Germany) detection kits. The two sample t tests between percents for differences between inflammatory and healthy sites and kappa statistics for the agreement of both systems were used. At the inflammatory sites, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were significantly more frequently detected by StomaGene® while Fusobacterium nucleatum, A. actinomycetemcomitans, Tanarella forsythia and Prevotella intermedia were significantly more frequently identified by ParoCheck® test. The agreement between the two detection systems was substantial for A. actinomycetemcomitans and F. nucleatum in the samples collected from inflamed sites and only for F. nucleatum from clinically healthy sites. Therefore, we recommend that the same system should be used when the same patient is examined repeatedly.
authors.affiliation: Clinic of Stomatology, Department of Paediatric Dentistry, St. Anne's Faculty Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Pavla
authors.initials: P
authors.lastname: Pantuckova
authors.affiliation: Clinic of Stomatology, Department of Paediatric Dentistry, St. Anne's Faculty Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic.|RECETOX, Research Centre for Toxic Compounds in the Environment, Masaryk University, Brno, Czech Republic.
authors.forename: Michaela
authors.initials: M
authors.lastname: Bartosova
authors.affiliation: School of Dental Medicine, the 1st Faculty of Medicine, Charles University, Prague, Czech Republic. broukal@vus.cz.
authors.forename: Zdenek
authors.identifier: http://orcid.org/0000-0002-5421-8625
authors.initials: Z
authors.lastname: Broukal
authors.affiliation: Clinic of Stomatology, Department of Paediatric Dentistry, St. Anne's Faculty Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Kukletova
authors.affiliation: Clinic of Stomatology, Department of Paediatric Dentistry, St. Anne's Faculty Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic.|Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Lydie Izakovicova
authors.initials: LI
authors.lastname: Holla
mesh_terms: D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000150:complications / Q000382:microbiology*; D001431:Bacteriological Techniques; D002675:Child, Preschool; D003731:Dental Caries / Q000150:complications / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D025202:Molecular Diagnostic Techniques; D010518:Periodontitis / Q000382:microbiology*