title: Obtusifoliol related steroids from Euphorbia sogdiana with cell growth inhibitory activity and apoptotic effects on breast cancer cells (MCF-7 and MDA-MB231).
abstract: From the aerial parts of Euphorbia sogdiana Popov, obtusifoliol (1) and two related steroids (2-3) have been isolated and characterized along with a known cycloartane derivative (4). The chemical structure of the obtusifoliol-related compounds, obtained by 1D and 2D NMR, and MS measurements, have been determined as: 3β,7α-dihydroxy-4α,14α-dimethyl-5α-ergosta-8,24(28)-diene-11-one (2) and 3β-hydroxy-4α,14α-dimethyl-5α-ergosta-8,24(28)-diene-1-one (3). Compound 2 has been previously isolated from Euphorbia chamaesyce while compound 3 was never reported before. The isolated compounds 1-4 were subjected to cytotoxic tests on the breast cancer cells, MCF-7 and MDA-MB231. Further pharmacological tests on the more active compounds 2 and 3 indicated their action to be related to cell growth inhibitory activity and apoptotic effects on the tested cells.
authors.affiliation: Department of Biochemistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Aghaei
authors.affiliation: Department of Pharmacognosy, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Yazdiniapour
authors.affiliation: Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. Electronic address: Ghannadian@gmail.com.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ghanadian
authors.affiliation: Department of Pharmacognosy, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Zolfaghari
authors.affiliation: Dipartimento di Agraria, Università di Napoli Federico II, Portici, Napoli, Italy. Electronic address: lanzotti@unina.it.
authors.forename: Virginia
authors.initials: V
authors.lastname: Lanzotti
authors.affiliation: Department of Pharmacognosy, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Mirsafaee
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002775:Cholestadienols / Q000737:chemistry*; D028481:Euphorbia / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013256:Steroids / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D002775:Cholestadienols; D013256:Steroids; C008693:obtusifoliol
keywords: 4α-Methyl sterols; Anticancer; Apoptosis; Breast cancer; Euphorbia sogdiana; Obtusifoliol analogues