title: Repair of segmental radial defects in dogs using tailor-made titanium mesh cages with plates combined with calcium phosphate granules and basic fibroblast growth factor-binding ion complex gel.
abstract: Repair of large segmental defects of long bones are a tremendous challenge that calls for a novel approach to supporting immediate weight bearing and bone regeneration. This study investigated the functional and biological characteristics of a combination of a tailor-made titanium mesh cage with a plate (tTMCP) with tetrapod-shaped alpha tricalcium phosphate granules (TB) and basic fibroblast growth factor (bFGF)-binding ion complex gel (f-IC gel) to repair 20-mm segmental radial defects in dogs. The defects were created surgically in 18 adult beagle dogs and treated by implantation of tTMCPs with TB with (TB-gel group) or without (TB group) f-IC gel. Each tTMCP fitted the defect well, and all dogs could bear weight on the affected limb immediately after surgery. Dogs were euthanized 4, 8 and 24 weeks after implantation. Histomorphometry showed greater infiltration of new vessels and higher bone union rate in the TB-gel group than in the TB group. The lamellar bone volume and mineral apposition rate did not differ significantly between the groups, indicating that neovascularization may be the primary effect of f-IC gel on bone regeneration. This combination method which is tTMCP combined with TB and f-IC gel, would be useful for the treatment of segmental long bone defects.
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.|Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Muneki
authors.initials: M
authors.lastname: Honnami
authors.affiliation: Division of Clinical Biotechnology, Graduate School of Medicine, Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Sungjin
authors.initials: S
authors.lastname: Choi
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: I-Li
authors.initials: IL
authors.lastname: Liu
authors.affiliation: Division of Tissue Engineering, Department of Vascular Regeneration, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.|Nanoscale Materials Division, Biomaterials Unit, Smart Biomaterials Group, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Kamimura
authors.affiliation: Nanoscale Materials Division, Biomaterials Unit, Smart Biomaterials Group, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Taguchi
authors.affiliation: Matsuura Machinery Corporation, 1-1 Urushihara-cho, Fukui, 910-8530, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Ichimura
authors.affiliation: Matsuura Machinery Corporation, 1-1 Urushihara-cho, Fukui, 910-8530, Japan.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Urushisaki
authors.affiliation: Division of Clinical Biotechnology, Graduate School of Medicine, Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Hojo
authors.affiliation: Division of Clinical Biotechnology, Graduate School of Medicine, Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Shimohata
authors.affiliation: Division of Clinical Biotechnology, Graduate School of Medicine, Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ohba
authors.affiliation: Matsuura Machinery Corporation, 1-1 Urushihara-cho, Fukui, 910-8530, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Amaya
authors.affiliation: Division of Tissue Engineering, Department of Vascular Regeneration, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Koyama
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Nishimura
authors.affiliation: Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.|Division of Clinical Biotechnology, Graduate School of Medicine, Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Ung-Il
authors.initials: UI
authors.lastname: Chung
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sasaki
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan. amm@mail.ecc.u-tokyo.ac.jp.
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
mesh_terms: D000818:Animals; D001860:Bone Plates; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D002130:Calcium Phosphates / Q000627:therapeutic use; D004285:Dogs; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use; D011884:Radius / Q000601:surgery*; D014025:Titanium; D016474:Weight-Bearing; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
chemical_list: D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; D016222:Fibroblast Growth Factor 2; C020243:calcium phosphate; D014025:Titanium
keywords: Fibroblast growth factor; Rapid prototyping; Segmental bone defect; Tetrabone®; Titanium mesh cage