title: Insight into the messenger role of reactive oxygen intermediates in immunostimulated hemocytes from the scallop Argopecten purpuratus.
abstract: Reactive oxygen intermediates (ROI) are metabolites produced by aerobic cells which have been linked to oxidative stress. Evidence reported in vertebrates indicates that ROI can also act as messengers in a variety of cellular signaling pathways, including those involved in innate immunity. In a recent study, an inhibitor of NF-kB transcription factors was identified in the scallop Argopecten purpuratus, and its functional characterization suggested that it may regulate the expression of the big defensin antimicrobial peptide ApBD1. In order to give new insights into the messenger role of ROI in the immune response of bivalve mollusks, the effect of ROI production on gene transcription of ApBD1 was assessed in A. purpuratus. The results showed that 48 h-cultured hemocytes were able to display phagocytic activity and ROI production in response to the β-glucan zymosan. The immune stimulation also induced the transcription of ApBD1, which was upregulated in cultured hemocytes. After neutralizing the ROI produced by the stimulated hemocytes with the antioxidant trolox, the transcription of ApBD1 was reduced near to base levels. The results suggest a potential messenger role of intracellular ROI on the regulation of ApBD1 transcription during the immune response of scallops.
authors.affiliation: Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, 2373223 Valparaíso, Chile.
authors.forename: Daniel
authors.initials: D
authors.lastname: Oyanedel
authors.affiliation: Laboratorio de Fisiología y Genética Marina (FIGEMA), Centro de Estudios Avanzados en Zonas Áridas (CEAZA), Universidad Católica Del Norte, 1781421 Coquimbo, Chile.
authors.forename: Roxana
authors.initials: R
authors.lastname: Gonzalez
authors.affiliation: Laboratorio de Fisiología y Genética Marina (FIGEMA), Centro de Estudios Avanzados en Zonas Áridas (CEAZA), Universidad Católica Del Norte, 1781421 Coquimbo, Chile.
authors.forename: Katherina
authors.initials: K
authors.lastname: Brokordt
authors.affiliation: Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, 2373223 Valparaíso, Chile. Electronic address: paulina.schmitt@pucv.cl.
authors.forename: Paulina
authors.initials: P
authors.lastname: Schmitt
authors.affiliation: Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, 2373223 Valparaíso, Chile.
authors.forename: Luis
authors.initials: L
authors.lastname: Mercado
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology; D023082:Defensins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D006434:Hemocytes / Q000276:immunology*; D007113:Immunity, Innate; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress; D049897:Pectinidae / Q000276:immunology*; D010587:Phagocytosis; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D015054:Zymosan / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D002839:Chromans; D023082:Defensins; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D015054:Zymosan; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
keywords: Antimicrobial peptide; Big defensin; Immune regulation; Invertebrate immunity; Reactive oxygen intermediates; Scallop