title: MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression.
abstract: Brucella may establish chronic infection by regulating the expression of miRNAs. However, the role of miRNAs in modulating the intracellular growth of Brucella remains unclear.
  
  
  
  In this study, we show that Brucella. abortus infection leads to downregulation of miR-125b-5p in macrophages. We establish that miR-125b-5p targets A20, an inhibitor of the NF-kB activation. Additionally, expression of miR-125b-5p decreases A20 expression in B. abortus-infected macrophages and leads to NF-kB activation and increased production of TNFα. Furthermore, B. abortus survival is attenuated in the presence of miR-125b-5p.
  
  
  
  These results uncover a role for miR-125b-5p in the regulation of B. abortus intracellular survival via the control of A20 expression.
authors.affiliation: Central Laboratory, The Second Hospital of Jilin University, Changchun, 130041, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Sun
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
authors.forename: Wanchun
authors.initials: W
authors.lastname: Sun
authors.affiliation: Central Laboratory, The Second Hospital of Jilin University, Changchun, 130041, China. Qishengpeng@yahoo.com.|Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China. Qishengpeng@yahoo.com.
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Peng
mesh_terms: D000818:Animals; D002003:Brucella abortus / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D002006:Brucellosis / Q000382:microbiology*; D003593:Cytoplasm / Q000187:drug effects* / Q000378:metabolism; D015536:Down-Regulation; D015964:Gene Expression Regulation, Bacterial; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000235:genetics*; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D000067996:RAW 264.7 Cells / Q000382:microbiology; D013534:Survival / Q000502:physiology*; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3 / Q000187:drug effects* / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3; C513261:Tnfaip3 protein, mouse
keywords: A20; Brucella abortus; Macrophage; MiR-125