title: HBME-1 is expressed by erythroid precursors in early maturation stage and can be a valuable tool for evaluation of dyserythropoiesis in bone marrow core biopsy specimens.
abstract: The reaction of Hector Battifora mesothelial epitope-1 (HBME-1) antibody with scattered pronormoblasts in normal bone marrow core biopsy specimens has been reported. This study evaluated the immunohistochemical profile of HBME-1 in a panel of 52 normal, dyserythropoietic and neoplastic marrow samples. We compared the staining property of HBME-1 with that of the commonly used erythroid marker, glycophorin A (CD235a) and in each case, we semi-quantitatively evaluated the HBME-1/CD235a-positive cells ratio. In normal samples, HBME-1 labelled scattered immature erythroid precursors. In dyserythropoietic specimens, HBME-1 stained nucleated erythroid precursors in varying degrees, from pronormoblast through normoblast stages, with the highest intensity in immature forms. Overall, the cellular background of non-erythroid progenitors, erythrocytes and neoplastic cells did not react with HBME-1, except in leukaemia cases with myelodysplasia-related changes. Our study shows that HBME-1 is a useful marker to identify immature erythroid precursors and that an HBME-1/CD235a-positive cells ratio ≥10% is associated with dyserythropoiesis.
authors.affiliation: Department of Pathology, Avicenne Hospital, Bobigny, France.
authors.forename: Riad
authors.initials: R
authors.lastname: Arana
authors.affiliation: Department of Biological Haematology, Avicenne Hospital, Bobigny, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lusina
authors.affiliation: Department of Clinical Haematology, Avicenne Hospital, Bobigny, France.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Braun
authors.affiliation: Department of Biological Haematology, Avicenne Hospital, Bobigny, France University of Paris 13, Bobigny, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Letestu
authors.affiliation: Department of Clinical Haematology, Avicenne Hospital, Bobigny, France University of Paris 13, Bobigny, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Gardin
authors.affiliation: Department of Pathology, Avicenne Hospital, Bobigny, France University of Paris 13, Bobigny, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Martin
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D014408:Biomarkers, Tumor / Q000276:immunology / Q000378:metabolism*; D062005:Biopsy, Large-Core Needle; D001853:Bone Marrow / Q000473:pathology*; D015672:Erythroid Precursor Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D006021:Glycophorins / Q000378:metabolism; D006801:Humans; D009190:Myelodysplastic Syndromes / Q000175:diagnosis / Q000378:metabolism*; D012189:Retrospective Studies; D000951:Antigens, Neoplasm
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D006021:Glycophorins; C432845:HBME-1 antigen; D000951:Antigens, Neoplasm
keywords: HEMATOPATHOLOGY; HISTOPATHOLOGY; IMMUNOHISTOCHEMISTRY