title: [Antiviral activities against influenza virus (FM1) of bioactive fractions and representative compounds extracted from Banlangen (Radix Isatidis)].
abstract: OBJECTIVE
  To study the antiviral activities of clemastanin B (CB), epigoitrin, phenylpropanoids portion (PEP) and the mixture of phenylpropanoids, alkaloids and organic acid fractions (PEP+ALK+OA) from Banlangen (Radix Isatidis).
  
  
  METHODS
  The experiment consisted of four parts: therapeutic action, prophylaxsis action, inhibition of virus attachment, and direct virucidal action. Cytopathic effect (CPE) and 3-(4,5-Dimethylthiazol-2-yI)-2,5-diphenyltetrazolium (MTT) were used to assess antiviral activity.
  
  
  RESULTS
  CB, epigoitrin, PEP and PEP + ALK +OA fractions from Banlangen (Radix Isatidis) extract significantly increased the viability of MDCK cells pre-infected with the virus compared with the virus control group in all the dilutions (P < 0.01). Pretreated with either pure compounds or chemical fractions of Banlangen (Radix Isatidis) extract in all the dilutions significantly improved the viability of MDCK cells (P < 0.01). The inhibition of virus absorption to the host cells by CB, epigoitrin and PEP was in a dose dependent manner.
  
  
  CONCLUSIONS
  CB, epigoitrin, PEP and PEP+ ALK+ OA exert their anti-influenza activity by inhibiting the virus multiplication, prophylaxsis and blocking the virus attachment. The primary mode of action of PEP and PEP + ALK + OA is the inhibition of virus replication. The inhibitory effects on virus attachment and multiplication are the main modes for epigoitrin.
authors.forename: Ping
authors.initials: P
authors.lastname: Xiao
authors.forename: Weiyang
authors.initials: W
authors.lastname: Ye
authors.forename: Jianwei
authors.initials: J
authors.lastname: Chen
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D007251:Influenza, Human / Q000188:drug therapy / Q000821:virology*; D009975:Orthomyxoviridae / Q000187:drug effects* / Q000502:physiology
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; C540579:banlangen