title: High-risk human papilloma virus genotypes in cervical carcinoma of Serbian women: Distribution and association with pathohistological findings.
abstract: A significant role of high-risk Human papilloma viruses (HR HPV) in the development of cervical carcinoma is well known. HR HPV 16 and 18 account for approximately 70% of all cases of cervical cancer worldwide. The incidence of cervical cancer in Serbia, is one of the highest in Europe. The aim of our study was to investigate the distribution of HR HPV types in cervical carcinoma of Serbian women, as well as association between the HPV types and pathohistological findings. The study included 80 archival cervical cancer tissues from the same number of patients. The presence of HPV DNA was determined using MY09/MY11 primers for L1 gene and GP1/GP2 primers for E1 gene. HPV was detected in 78.75% tissues. HR HPV genotypes found in the decreasing order of frequency were: HPV16 (80.39%), HPV33 (7.84%), HPV58 (5.88%), HPV18 (1.96%), HPV45 (1.96%) and HPV53 (1.96%). The examined tissues were 91.25% squamous cell carcinomas and 8.75% adenocarcinoma. The high frequency of HPV 16 was observed in both types of carcinoma (80.8% and 75%, respectively) while the prevalence of HPV18 was low. These results may contribute to the implementation of cervical carcinoma prevention program in Serbia, including the selection of the most appropriate vaccine and immunization program.
authors.affiliation: Medicines and Medical Devices Agency of Serbia, 11000, Belgrade, Serbia.
authors.forename: Miodrag
authors.initials: M
authors.lastname: Stamenković
authors.affiliation: Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000, Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Knežević
authors.affiliation: Department of Essential Medicines and Health Products, Health Systems and Innovation, World Health Organization, 1211, Geneva, Switzerland. Electronic address: knezevici@who.int.
authors.forename: Ivana
authors.initials: I
authors.lastname: Knežević
authors.affiliation: "Narodni Front" University Clinic of Gynecology and Obstetrics, Faculty of Medicine, University of Belgrade, 11000, Belgrade, Serbia.
authors.forename: Igor
authors.initials: I
authors.lastname: Kuzmanović
authors.affiliation: Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000, Belgrade, Serbia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Karalić
authors.affiliation: Department for Clinical Pathology, Clinical-Hospital Center "Zemun", Faculty of Medicine, University of Belgrade, 11000, Belgrade, Serbia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Milenković
authors.affiliation: Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000, Belgrade, Serbia.
authors.forename: Tanja
authors.initials: T
authors.lastname: Jovanović
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000235:genetics / Q000821:virology; D012307:Risk Factors; D055771:Serbia; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000821:virology
chemical_list: D004279:DNA, Viral; D009856:Oncogene Proteins, Viral
keywords: Cervical carcinoma; HPV genotype; Prevention programme; Tumor stage