title: Abnormal histone acetylation of CD8+T cells in patients with severe aplastic anemia.
abstract: Severe aplastic anemia (SAA) is a rare autoimmune disease characterized by severe pancytopenia and bone marrow failure, which is caused by activated T lymphocytes. In the present study, we evaluated histone H3 acetylation levels of bone marrow CD8+T cells in SAA patients, and analyzed its correlation with clinical condition parameters. We found that the percentages of CD8+T cell histone H3 acetylation in patients with untreated SAA, recovering SAA (R-SAA) and normal control, were 1.21 ± 0.08, 1.05 ± 0.36, and 1.00 ± 0.41, respectively, with no significant statistical differences. However, the amount of CD8+T cell histone H3 acetylation from untreated SAA was 176.21 ± 32.22 μg/mg protein, which was significantly higher than that of complete response (CR)-SAA (104.29 ± 62.06 μg/mg protein) and normal control (133.94 ± 56.27 μg/mg protein) (P < 0.05) groups. Moreover, histone H3 acetylation amount of CD8+T cell was significantly and negatively correlated with absolute neutrophil count, proportion of reticulocytes, ratio of CD4+to CD8+T cell in peripheral blood, and percentage of bone marrow erythroid (P < 0.05). To some extent, it also negatively correlated with hemoglobin level, platelet count, percentage of bone marrow granulocyte, and megakaryocyte count. Abnormal histone H3 acetylation of CD8+T cells may thus play a role in the immune pathogenesis of SAA.
authors.affiliation: Department of Hematology, General Hospital of Tianjin Medical University, 154 Anshandao, Heping District, Tianjin, 300052, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Qi
authors.affiliation: Department of Hematology, General Hospital of Tianjin Medical University, 154 Anshandao, Heping District, Tianjin, 300052, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Hematology, General Hospital of Tianjin Medical University, 154 Anshandao, Heping District, Tianjin, 300052, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Hematology, General Hospital of Tianjin Medical University, 154 Anshandao, Heping District, Tianjin, 300052, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.affiliation: Department of Hematology, General Hospital of Tianjin Medical University, 154 Anshandao, Heping District, Tianjin, 300052, China.
authors.forename: Huaquan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Hematology, General Hospital of Tianjin Medical University, 154 Anshandao, Heping District, Tianjin, 300052, China. shaozonghong@sina.com.
authors.forename: Zonghong
authors.initials: Z
authors.lastname: Shao
mesh_terms: D000107:Acetylation; D000741:Anemia, Aplastic / Q000276:immunology / Q000473:pathology*; D001772:Blood Cell Count; D001853:Bone Marrow / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D002452:Cell Count; D006657:Histones / Q000378:metabolism*; D006801:Humans
chemical_list: D006657:Histones
keywords: Acetylation; Aplastic anemia; CD8+ T cell; Histone