title: Fungal Isolate Optimized for Biogenesis of Silver Nanoparticles with Enhanced Colloidal Stability.
abstract: Understanding the synthesis and properties of nanomaterials is critical for reliable applications. Biological systems, such as fungi, have been described as a "green" alternative to synthesis, yet knowledge gaps exist in terms of production variability, comparison with commercial products, and identifying a clear biological advantage over other synthesis methods. In this study, we evaluated 12 fungal isolates of Fusarium oxysporum for Ag nanoparticle production and characterized the resultant biologically produced (biogenic) nanoparticles. We show evidence that isolate selection, temperature, and pH can influence the quantity, size, and shape of nanoparticles. All F. oxysporum isolates produced Ag nanoparticles, but in varied quantities. Increasing incubation temperature increased the quantity, yet nanoparticle diameter was inversely related to temperature. Variations in pH predominately influenced nanoparticle morphology. A direct comparison with commercial, chemically produced Ag nanoparticles yielded physical similarities; however, important differences in surface chemistry are observed. Biogenic nanoparticles show a greater degree of colloidal stability in high-ionic-strength solutions, pointing to a biological advantage associated with the fungal produced layer (corona) surrounding the nanoparticles. It is clear that understanding the organic layer and interfacial interactions will be beneficial in developing innovative applications, particularly in the field of biosensing.
authors.affiliation: Ecosystems and Plant Sciences, Alberta Innovates Technology Futures, P.O. Bag 4000, Vegreville, Alberta, Canada T9C 1T4.
authors.forename: Sunil
authors.initials: S
authors.lastname: Rajput
authors.affiliation: Ecosystems and Plant Sciences, Alberta Innovates Technology Futures, P.O. Bag 4000, Vegreville, Alberta, Canada T9C 1T4.
authors.forename: Rodney
authors.initials: R
authors.lastname: Werezuk
authors.affiliation: Ecosystems and Plant Sciences, Alberta Innovates Technology Futures, P.O. Bag 4000, Vegreville, Alberta, Canada T9C 1T4.
authors.forename: Ralph M
authors.initials: RM
authors.lastname: Lange
authors.forename: Mark T
authors.initials: MT
authors.lastname: McDermott
mesh_terms: D003102:Colloids; D004355:Drug Stability; D000067493:Dynamic Light Scattering; D005670:Fusarium / Q000302:isolation & purification* / Q000378:metabolism* / Q000648:ultrastructure; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D010316:Particle Size; D012834:Silver / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature
chemical_list: D003102:Colloids; D012834:Silver