title: The Anti-Adipogenic Activity of a New Cultivar,Pleurotus eryngiivar.ferulae'Beesan No. 2', through Down-Regulation of PPAR γ and C/EBP α in 3T3-L1 Cells.
abstract: Adipogenesis is one of the cellular processes and a highly controlled program. Nowadays, inhibition of adipogenesis has received attention as an effective way to regulate obesity. In the current study, we investigated the inhibition effect of a chloroform extract ofPleurotus eryngiivar.ferulae'Beesan No. 2' (CEBT) on adipogenesis in 3T3-L1 murine preadipocytes.Pleurotus eryngiivar.ferulaeis one of many varieties of King oyster mushroom and has been reported to have various biological activities, including antitumor and anti-inflammation effects. Biological activities of 'Beesan No. 2', a new cultivar ofPleurotus eryngiivar.ferulae, have not yet been reported. In this study, we found that CEBT suppressed adipogenesis in 3T3-L1 cells through inhibition of key adipogenic transcription factors, such as peroxisome proliferator-activated receptor γ and CCAAT/enhancer binding protein α. Additionally, CEBT reduced the expression of the IRS/PI3K/Akt signaling pathway and its downstream factors, including mammalian target of rapamycin and p70S6 kinase, which stimulate adipogenesis. Furthermore, β-catenin, a suppressor of adipogenesis, was increased in CEBT-treated cells. These results indicate thatPleurotus eryngiivar.ferulae'Beesan No. 2' effectively inhibited adipogenesis, so this mushroom has potential as an anti-obesity food and drug.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Min-Jae
authors.initials: MJ
authors.lastname: Kang
authors.affiliation: Department of Life Science and Environmental Biochemistry, College of Natural Resource and Life Sciences, Pusan National University, Miryang 50463, Republic of Korea.
authors.forename: Keun Ki
authors.initials: KK
authors.lastname: Kim
authors.affiliation: Blue-Bio Industry Regional Innovation Center, Dongeui University, Busan 47340, Republic of Korea.
authors.forename: Byoung Yil
authors.initials: BY
authors.lastname: Son
authors.affiliation: Department of Biotechnology and Bioengineering, Dongeui University, Busan 47340, Republic of Korea.
authors.forename: Soo-Wan
authors.initials: SW
authors.lastname: Nam
authors.affiliation: Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of Korea.
authors.forename: Pyung-Gyun
authors.initials: PG
authors.lastname: Shin
authors.affiliation: Department of Microbiology, College of Natural Sciences, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Gun-Do
authors.initials: GD
authors.lastname: Kim
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects*; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D020076:Pleurotus / Q000737:chemistry* / Q000254:growth & development; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000235:genetics / Q000378:metabolism; D014675:Vegetables / Q000737:chemistry*
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D047495:PPAR gamma; D010936:Plant Extracts; D038762:Ribosomal Protein S6 Kinases, 70-kDa
keywords: Adipocytes; adipogenesis; lipid metabolism; mushroom; obesity