title: An Improved PCR-RFLP Assay for the Detection of a Polymorphism of PLIN1 Gene.
abstract: BACKGROUND
  In recent research, it has been shown that there have been variants of rs894160 within the PLINI gene which have been associated with obesity, type 2 diabetes, and other diseases. But the isoschizomers such as the Mn1I enzyme required for the detection of this polymorphism are expensive.
  
  
  METHODS
  The study used an improved PCR-RFLP method with mismatched base for detection of the single nucleotide polymorphism rs894160.
  
  
  RESULTS
  After detecting 550 Chinese Han individuals, the genotype frequencies were 26.0% for AA, 50.0% for AG, and 24.0% for GG. The allelic frequencies were 51.0% for A and 49.0% for G. The PCR results were confirmed by DNA sequencing. The chi2 test showed the genotype and allele frequencies of PLIN1 do not deviate from Hardy-Weinberg equilibrium, and the sequences of amplified products were consistent with the one published in Genbank with the exception of mismatched base.
  
  
  CONCLUSIONS
  Based on the PCR with mismatched primers we designed, the PLIN1 polymorphisms could be identified effectively.
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Duan
authors.forename: Chunyang
authors.initials: C
authors.lastname: Li
authors.forename: Tuanwei
authors.initials: T
authors.lastname: Wang
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Feng
authors.forename: Liuxin
authors.initials: L
authors.lastname: Cui
authors.forename: Wu
authors.initials: W
authors.lastname: Yao
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D044466:Asian People / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D016009:Chi-Square Distribution; D002681:China; D017931:DNA Primers; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011237:Predictive Value of Tests
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; C000602978:PLIN1 protein, human; D000070778:Perilipin-1; D010750:Phosphoproteins