title: Metabolic activities of five botryticides against Botrytis cinerea examined using the Biolog FF MicroPlate.
abstract: Tobacco grey mold caused by Botrytis cinerea is an important fungal disease worldwide. Boscalid, carbendazim, iprodione, pyrimethanil and propiconazole are representative botryticides for grey mold management. This research investigated the sensitivities of B. cinerea from tobacco to these chemicals using the Biolog FF Microplate. All five chemicals showed inhibitory activity, with average EC50 values of 0.94, 0.05, 0.50, 0.61 and 0.31 μg ml(-1), respectively. B. cinerea metabolized 96.8% of tested carbon sources, including 29 effectively and 33 moderately, but the metabolic fingerprints differed under pressures imposed by these botryticides. For boscalid, B. cinerea was unable to metabolize many substrates related to tricarboxylic acid cycle. For carbendazim, carbon sources related to glycolysis were not metabolized. For iprodione, use of most carbon substrates was weakly inhibited, and the metabolic profile was similar to that of the control. For propiconazole, no carbon substrates were metabolized and the physiological and biochemical functions of the pathogen were totally inhibited. These findings provide useful information on metabolic activities of these botryticides, and may lead to future applications of the Biolog FF Microplate for examining metabolic effects of other fungicides on other fungi, as well as providing a metabolic fingerprint of B. cinerea that could be useful for identification.
authors.affiliation: Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, Guiyang 550081, P.R. China.
authors.forename: Hancheng
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Life Science, Yangtze University, Jingzhou 434025, P.R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Life Science, Yangtze University, Jingzhou 434025, P.R. China.
authors.forename: Licui
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Environmental Sciences, University of Guelph, Guelph, ON, N1G2W1, Canada.
authors.forename: Tom
authors.initials: T
authors.lastname: Hsiang
authors.affiliation: Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, Guiyang 550081, P.R. China.
authors.forename: Maosheng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, Guiyang 550081, P.R. China.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Shang
authors.affiliation: College of Life Science, Yangtze University, Jingzhou 434025, P.R. China.
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Yu
mesh_terms: D020171:Botrytis / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D005659:Fungicides, Industrial / Q000378:metabolism*; D008660:Metabolism / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D014026:Nicotiana / Q000382:microbiology
chemical_list: D005659:Fungicides, Industrial; D002244:Carbon