title: Zoonotic Enterocytozoon bieneusi genotypes found in brazilian sheep.
abstract: The presence of Enterocytozoon bieneusi in sheep has been reported in only three countries worldwide. The present study has found E. bieneusi in Brazilian sheep for the first time; in 24/125 (19.2%) fecal samples by PCR and on 8/10 (80%) farms from three diverse locations. A significantly greater number of lambs (34.1%) were found infected than older sheep (11.1%) (P=0.0036); most of the lambs were less than 6months of age. Farms with an intensive production system had a lower infection rate (10.5%) of infection than semi-intensive farms (23%), but this difference was not statistically significant. Sequencing analysis of the internal transcribed spacer (ITS) region of the rRNA gene revealed four known E. bieneusi genotypes (BEB6, BEB7, I, and LW1) and two novel genotypes (BEB18 and BEB19). Genotypes LW1 and BEB19 clustered within designated zoonotic Group 1 while genotypes BEB6, BEB7, I, and BEB18, and clustered within Group 2. BEB6 was the most prevalent (45.8%), followed by BEB7 (33.3%). Genotypes BEB6, I, and LW1 are zoonotic and can pose a risk to human health for immunocompromised individuals.
authors.affiliation: Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, 10300 Baltimore Avenue, BARC-East, Building 173, Beltsville, MD 20705, USA; Departamento de Parasitologia Animal, Instituto de Veterinária, UFRRJ, BR-465 km 7, 23897-970, Seropédica, Rio de Janeiro, Brazil.
authors.forename: Vagner Ricardo da Silva
authors.initials: VRDS
authors.lastname: Fiuza
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, UFRRJ, BR-465 km 7, 23897-970, Seropédica, Rio de Janeiro, Brazil.
authors.forename: Carlos Wilson Gomes
authors.initials: CWG
authors.lastname: Lopes
authors.affiliation: Laboratório Multiusuário de Apoio à Pesquisa em Nefrologia e Ciências Médicas, HUAP, UFF, 24033-900, Niterói, Rio de Janeiro, Brazil.
authors.forename: Rachel Ingrid Juliboni
authors.initials: RIJ
authors.lastname: Cosendey
authors.affiliation: Laboratório de Sanidade Animal, UENF, 28013-602, Campos dos Goytacazes, Rio de Janeiro, Brazil.
authors.forename: Francisco Carlos Rodrigues
authors.initials: FCR
authors.lastname: de Oliveira
authors.affiliation: Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, 10300 Baltimore Avenue, BARC-East, Building 173, Beltsville, MD 20705, USA.
authors.forename: Ronald
authors.initials: R
authors.lastname: Fayer
authors.affiliation: Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, 10300 Baltimore Avenue, BARC-East, Building 173, Beltsville, MD 20705, USA. Electronic address: monica.santin-duran@ars.usda.gov.
authors.forename: Monica
authors.initials: M
authors.lastname: Santín
mesh_terms: D000375:Aging; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D021862:Enterocytozoon / Q000145:classification / Q000235:genetics*; D005243:Feces / Q000469:parasitology; D005838:Genotype; D006801:Humans; D016881:Microsporidiosis / Q000382:microbiology / Q000662:veterinary*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology*; D015047:Zoonoses
keywords: E. bieneusi; Genotyping; Microsporidia; PCR; Public health; Zoonosis