title: TRAIP regulates replication fork recovery and progression via PCNA.
abstract: PCNA is a central scaffold that coordinately assembles replication and repair machineries at DNA replication forks for faithful genome duplication. Here, we describe TRAIP (RNF206) as a novel PCNA-interacting factor that has important roles during mammalian replicative stress responses. We show that TRAIP encodes a nucleolar protein that migrates to stalled replication forks, and that this is accomplished by its targeting of PCNA via an evolutionarily conserved PIP box on its C terminus. Accordingly, inactivation of TRAIP or its interaction with the PCNA clamp compromised replication fork recovery and progression, and leads to chromosome instability. Together, our findings establish TRAIP as a component of the mammalian replicative stress response network, and implicate the TRAIP-PCNA axis in recovery of stalled replication forks.
authors.affiliation: School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong S.A.R., China; Centre for Cancer Research, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong S.A.R., China.
authors.forename: Wanjuan
authors.initials: W
authors.lastname: Feng
authors.affiliation: School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong S.A.R., China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Life Sciences Institute, Zhejiang University, Zhejiang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Life Sciences, South China Agricultural University, Guangzhou, China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Deng
authors.affiliation: School of Life Sciences, University of Science of Technology of China , Hefei, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Zang
authors.affiliation: School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong S.A.R., China; Centre for Cancer Research, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong S.A.R., China; State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Pokfulam, Hong Kong S.A.R., China.
authors.forename: Michael Shing-Yan
authors.initials: MS
authors.lastname: Huen
keywords: DNA damage; PCNA; RNF206; TRAIP; hydroxyurea; replication