title: Epitope analysis of DQ6-reactive antibodies in sera from a DQ6-positive transplant candidate sensitized during pregnancy.
abstract: This case report describes DQ6-reactive serum antibody reactivity in a patient who types as DQ6. DNA typing showed DQB1*06:09 on the antibody producer and serum reactivity with DQB1*06:01, *06:02 and *06:03 but not with *06:04 and *06:09. HLAMatchmaker serum analysis showed antibody reactivity with a new antibody-verified 85VA eplet on DQB but additional reactivity with DQB1*02:01 could not be readily interpreted. After applying the nonself-self algorithm of HLA immunogenicity we have identified a new DQB epitope structurally described as 140A2+130R+135D and shared by DQB1*02:01 and DQB1*05:01 and DQB1*06:02 of the immunizer.
authors.affiliation: Histocompatibility and Immunogenetics Laboratory (HILA), Red Cross-Flanders, Mechelen, Belgium. Electronic address: liesbeth.daniels@rodekruis.be.
authors.forename: Liesbeth
authors.initials: L
authors.lastname: Daniëls
authors.affiliation: Histocompatibility and Immunogenetics Laboratory (HILA), Red Cross-Flanders, Mechelen, Belgium.
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Emonds
authors.affiliation: Department of Nephrology, Antwerp University Hospital, Edegem, Belgium.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Bosmans
authors.affiliation: Division of Transplantation Pathology, Thomas E. Starzl Transplantation Institute, University of Pittsburgh Medical Center, PA, USA.
authors.forename: Marilyn
authors.initials: M
authors.lastname: Marrari
authors.affiliation: Division of Transplantation Pathology, Thomas E. Starzl Transplantation Institute, University of Pittsburgh Medical Center, PA, USA.
authors.forename: Rene J
authors.initials: RJ
authors.lastname: Duquesnoy
mesh_terms: D000465:Algorithms; D000483:Alleles; D001324:Autoantigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018604:Epitope Mapping / Q000379:methods*; D005260:Female; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D006650:Histocompatibility Testing; D006801:Humans; D007114:Immunization; D007518:Isoantibodies / Q000097:blood; D007519:Isoantigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008875:Middle Aged; D011247:Pregnancy; D011487:Protein Conformation; D012042:Registries; D066027:Transplant Recipients; D014850:Waiting Lists
chemical_list: D001324:Autoantigens; D006683:HLA-DQ Antigens; C063831:HLA-DQ6 antigen; D007518:Isoantibodies; D007519:Isoantigens
keywords: Epitope; HLA; HLA antibodies; HLAMatchmaker; Nonself-self