title: Collecting Duct Renin Does Not Mediate DOCA-Salt Hypertension or Renal Injury.
abstract: Collecting duct (CD)-derived renin is involved in the hypertensive response to chronic angiotensin-II (Ang-II) administration. However, whether CD renin is involved in Ang-II independent hypertension is currently unknown. To begin to examine this, 12 week old male and female CD-specific renin knock out (KO) mice and their littermate controls were subjected to uni-nephrectomy followed by 2 weeks of deoxycorticosterone acetate (DOCA) infusion combined with a high salt diet. Radiotelemetric blood pressure (BP) was similar between KO and control mice at baseline; BP increased in both groups to a similar degree throughout the 2 weeks of DOCA-salt treatment. Urinary albumin excretion and plasma blood urea nitrogen were comparable between the two groups after DOCA-salt treatment. Fibrosis as assessed by Masson's Trichrome stain/Sirius Red stain and collagen-1 mRNA expression was similar between control and KO mice. Compared to baseline, DOCA-salt treatment decreased plasma renin concentration (PRC), urinary renin excretion and medullary renin mRNA expression in both floxed and CD renin KO mice with no detectable differences between the two groups. Further, in primary culture of rat inner medullary CD, aldosterone treatment did not change renin activity or total renin content. Taken together, these data suggest that CD derived renin does not play a role in DOCA-salt hypertension.
authors.affiliation: Division of Nephrology and Hypertension, University of Utah Health Sciences Center, Salt Lake City, Utah, United States of America.|Department of Nephrology, Second Affiliated Hospital of Soochow University, Soochow City, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Song
authors.affiliation: Division of Nephrology and Hypertension, University of Utah Health Sciences Center, Salt Lake City, Utah, United States of America.
authors.forename: Deborah
authors.initials: D
authors.lastname: Stuart
authors.affiliation: Division of Nephrology and Hypertension, University of Utah Health Sciences Center, Salt Lake City, Utah, United States of America.
authors.forename: Nikita
authors.initials: N
authors.lastname: Abraham
authors.affiliation: Division of Nephrology and Hypertension, University of Utah Health Sciences Center, Salt Lake City, Utah, United States of America.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Division of Nephrology and Hypertension, University of Utah Health Sciences Center, Salt Lake City, Utah, United States of America.
authors.forename: Shuping
authors.initials: S
authors.lastname: Wang
authors.affiliation: Division of Nephrology and Hypertension, University of Utah Health Sciences Center, Salt Lake City, Utah, United States of America.|Veterans Affairs Salt Lake City Health Care System, Salt Lake City, Utah, United States of America.
authors.forename: Tianxin
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Curt D
authors.initials: CD
authors.lastname: Sigmund
authors.affiliation: Division of Nephrology and Hypertension, University of Utah Health Sciences Center, Salt Lake City, Utah, United States of America.|Veterans Affairs Salt Lake City Health Care System, Salt Lake City, Utah, United States of America.
authors.forename: Donald E
authors.initials: DE
authors.lastname: Kohan
authors.affiliation: Division of Nephrology and Hypertension, University of Utah Health Sciences Center, Salt Lake City, Utah, United States of America.
authors.forename: Nirupama
authors.initials: N
authors.lastname: Ramkumar
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002478:Cells, Cultured; D064791:Desoxycorticosterone Acetate / Q000037:antagonists & inhibitors*; D006973:Hypertension / Q000139:chemically induced / Q000503:physiopathology*; D007668:Kidney / Q000293:injuries*; D007679:Kidney Medulla / Q000378:metabolism; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000235:genetics; D017207:Rats, Sprague-Dawley; D012083:Renin / Q000235:genetics / Q000502:physiology* / Q000652:urine
chemical_list: D012333:RNA, Messenger; D064791:Desoxycorticosterone Acetate; D012083:Renin