title: Recycled de-Oiled Algal Biomass Extract as a Feedstock for Boosting Biodiesel Production from Chlorella minutissima.
abstract: The investigation for the first time assesses the efficacy of recycled de-oiled algal biomass extract (DABE) as a cultivation media to boost lipid productivity in Chlorella minutissima and its comparison with Bold's basal media (BBM) used as control. Presence of organic carbon (3.8 ± 0.8 g/l) in recycled DABE resulted in rapid growth with twofold increase in biomass productivity as compared to BBM. These cells expressed four folds higher lipid productivity (126 ± 5.54 mg/l/d) as compared to BBM. Cells cultivated in recycled DABE showed large sized lipid droplets accumulating 54.12 % of lipid content. Decrement in carbohydrate (17.76 %) and protein content (28.12 %) with loss of photosynthetic pigments compared to BBM grown cells were also recorded. The fatty acid profiles of cells cultivated in recycled DABE revealed the dominance of C16:0 (39.66 %), C18:1 (29.41 %) and C18:0 (15.82 %), respectively. This model is self-sustained and aims at neutralizing excessive feedstock consumption by exploiting recycled de-oiled algal biomass for cultivation of microalgae, making the process cost effective.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, 247667, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Arora
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, 247667, India.
authors.forename: Alok
authors.initials: A
authors.lastname: Patel
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, 247667, India.
authors.forename: Parul A
authors.initials: PA
authors.lastname: Pruthi
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, 247667, India. vikasfbs@iitr.ernet.in.
authors.forename: Vikas
authors.initials: V
authors.lastname: Pruthi
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D048429:Cell Size / Q000187:drug effects; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000494:pharmacology; D004952:Esters / Q000378:metabolism; D009821:Oils / Q000302:isolation & purification*; D010860:Pigments, Biological / Q000378:metabolism; D059027:Recycling
chemical_list: D056804:Biofuels; D003470:Culture Media; D004952:Esters; D009821:Oils; D010860:Pigments, Biological
keywords: Biodiesel; Chlorella minutissima; De-oiled algal extract; Lipid productivity