title: Adjusting irradiance to enhance growth and lipid production of Chlorella vulgaris cultivated with monosodium glutamate wastewater.
abstract: Light is one of the most important factors affecting microalgae growth and biochemical composition. The influence of illumination on Chlorella vulgaris cultivated with diluted monosodium glutamate wastewater (MSGW) was investigated. Six progressive illumination intensities (0, 30, 90, 150, 200 and 300μmol·m(-2)s(-1)), were used for C. vulgaris cultivation at 25°C. Under 150μmol·m(-2)s(-1), the corresponding specific light intensity of 750×10(-6)μmol·m(-2)s(-1) per cell, algae obtained the maximum biomass concentration (1.46g·L(-1)) on the 7th day, which was 3.5 times of that under 0μmol·m(-2)s(-1), and the greatest average specific growth rate (0.79 d(-1)) in the first 7days. The results showed the importance role of light in mixotrophic growth of C. vulgaris. High light intensities of 200 and 300μmol·m(-2)s(-1) would inhibit microalgae growth to a certain degree. The algal lipid content was the greatest (30.5%) at 150μmol·m(-2)s(-1) light intensity, which was 2.42 times as high as that cultured in dark. The protein content of C. vulgaris decreased at high light intensities of 200 and 300μmol·m(-2)s(-1). The effect of irradiance on carbohydrate content was inversely correlated with that on protein. The available light at an appropriate intensity, not higher than 200μmol·m(-2)s(-1), was feasible for economical cultivation of C. vulgaris in MSGW.
authors.affiliation: School of Environmental Science and Engineering, Shandong University, 27 Shanda Nan Road, Jinan 250100, Shandong, China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Jiang
authors.affiliation: School of Environmental Science and Engineering, Shandong University, 27 Shanda Nan Road, Jinan 250100, Shandong, China; School of Biological Science and Technology, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan 250022, Shandong, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ji
authors.affiliation: School of Environmental Science and Engineering, Shandong University, 27 Shanda Nan Road, Jinan 250100, Shandong, China; Shandong Provincial Engineering Centre on Environmental Science and Technology, 17923 Jingshi Road, Jinan 250061, Shandong, China.
authors.forename: Wenrong
authors.initials: W
authors.lastname: Hu
authors.affiliation: School of Environmental Science and Engineering, Shandong University, 27 Shanda Nan Road, Jinan 250100, Shandong, China; Shandong Provincial Engineering Centre on Environmental Science and Technology, 17923 Jingshi Road, Jinan 250061, Shandong, China. Electronic address: haiyanhup@126.com.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Pei
authors.affiliation: School of Environmental Science and Engineering, Shandong University, 27 Shanda Nan Road, Jinan 250100, Shandong, China.
authors.forename: Changliang
authors.initials: C
authors.lastname: Nie
authors.affiliation: School of Environmental Science and Engineering, Shandong University, 27 Shanda Nan Road, Jinan 250100, Shandong, China.
authors.forename: Guixia
authors.initials: G
authors.lastname: Ma
authors.affiliation: School of Environmental Science and Engineering, Shandong University, 27 Shanda Nan Road, Jinan 250100, Shandong, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Song
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D018533:Biomass; D048408:Chlorella vulgaris / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008027:Light; D050356:Lipid Metabolism / Q000187:drug effects* / Q000528:radiation effects; D012970:Sodium Glutamate / Q000494:pharmacology*; D062065:Wastewater / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D012970:Sodium Glutamate; D062065:Wastewater
keywords: Biochemical composition; Chlorella vulgaris; Light intensity; Monosodium glutamate wastewater; Specific growth rate