title: Ion Mobility-Mass Spectrometry as a Tool for the Structural Characterization of Peptides Bearing Intramolecular Disulfide Bond(s).
abstract: Disulfide bonds are post-translationnal modifications that can be crucial for the stability and the biological activities of natural peptides. Considering the importance of these disulfide bond-containing peptides, the development of new techniques in order to characterize these modifications is of great interest. For this purpose, collision cross cections (CCS) of a large data set of 118 peptides (displaying various sequences) bearing zero, one, two, or three disulfide bond(s) have been measured in this study at different charge states using ion mobility-mass spectrometry. From an experimental point of view, CCS differences (ΔCCS) between peptides bearing various numbers of disulfide bonds and peptides having no disulfide bonds have been calculated. The ΔCCS calculations have also been applied to peptides bearing two disulfide bonds but different cysteine connectivities (Cys1-Cys2/Cys3-Cys4; Cys1-Cys3/Cys2-Cys4; Cys1-Cys4/Cys2-Cys3). The effect of the replacement of a proton by a potassium adduct on a peptidic structure has also been investigated. Graphical Abstract ᅟ.
authors.affiliation: Laboratory of Mass Spectrometry, University of Liege, Quartier Agora, Allée du six Aout 11, B-4000, Liege, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Massonnet
authors.affiliation: Laboratory of Mass Spectrometry, University of Liege, Quartier Agora, Allée du six Aout 11, B-4000, Liege, Belgium.
authors.forename: Jean R N
authors.initials: JR
authors.lastname: Haler
authors.affiliation: Commissariat à l'Energie Atomique, DRF/iBiTec-S/SIMOPRO, CE Saclay, 91191, Gif-sur-Yvette, France.
authors.forename: Gregory
authors.initials: G
authors.lastname: Upert
authors.affiliation: Laboratory of Mass Spectrometry, University of Liege, Quartier Agora, Allée du six Aout 11, B-4000, Liege, Belgium.
authors.forename: Michel
authors.initials: M
authors.lastname: Degueldre
authors.affiliation: Laboratory of Mass Spectrometry, University of Liege, Quartier Agora, Allée du six Aout 11, B-4000, Liege, Belgium.
authors.forename: Denis
authors.initials: D
authors.lastname: Morsa
authors.affiliation: Laboratory of Mass Spectrometry, University of Liege, Quartier Agora, Allée du six Aout 11, B-4000, Liege, Belgium.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Smargiasso
authors.affiliation: Commissariat à l'Energie Atomique, DRF/iBiTec-S/SIMOPRO, CE Saclay, 91191, Gif-sur-Yvette, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Mourier
authors.affiliation: Commissariat à l'Energie Atomique, DRF/iBiTec-S/SIMOPRO, CE Saclay, 91191, Gif-sur-Yvette, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gilles
authors.affiliation: Laboratory of Mass Spectrometry, University of Liege, Quartier Agora, Allée du six Aout 11, B-4000, Liege, Belgium.
authors.forename: Loïc
authors.initials: L
authors.lastname: Quinton
authors.affiliation: Laboratory of Mass Spectrometry, University of Liege, Quartier Agora, Allée du six Aout 11, B-4000, Liege, Belgium. e.depauw@ulg.ac.be.
authors.forename: Edwin
authors.initials: E
authors.lastname: De Pauw
mesh_terms: D000595:Amino Acid Sequence; D003545:Cysteine; D004220:Disulfides / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D011522:Protons
chemical_list: D004220:Disulfides; D010455:Peptides; D011522:Protons; D003545:Cysteine
keywords: Disulfide bond; Ion-mobility mass spectrometry; Peptides