title: N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier.
abstract: N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in Eukarya. Previously, hexose-bearing dolichol phosphate was detected in a S. acidocaldarius Bligh-Dyer lipid extract. Here, we used a specialized protocol for extracting lipid-linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N-glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N-glycosylation.
authors.affiliation: Department of Biochemistry, Duke University Medical Center, Durham, NC, USA.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Guan
authors.affiliation: Department of Life Sciences, Ben Gurion University of the Negev, Beersheva, Israel.
authors.forename: Antonia
authors.initials: A
authors.lastname: Delago
authors.affiliation: Molecular Biology of Archaea, Institute for Biology II-Microbiology, Albert-Ludwigs-University of Freiburg, Germany.
authors.forename: Phillip
authors.initials: P
authors.lastname: Nußbaum
authors.affiliation: Molecular Biology of Archaea, Institute for Biology II-Microbiology, Albert-Ludwigs-University of Freiburg, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Meyer
authors.affiliation: Molecular Biology of Archaea, Institute for Biology II-Microbiology, Albert-Ludwigs-University of Freiburg, Germany.
authors.forename: Sonja-Verena
authors.initials: SV
authors.lastname: Albers
authors.affiliation: Department of Life Sciences, Ben Gurion University of the Negev, Beersheva, Israel. jeichler@bgu.ac.il.
authors.forename: Jerry
authors.initials: J
authors.lastname: Eichler
mesh_terms: D019843:Archaeal Proteins / Q000378:metabolism*; D004288:Dolichol Phosphates / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D011499:Protein Processing, Post-Translational; D016945:Sulfolobus acidocaldarius / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D004288:Dolichol Phosphates
keywords: N-glycosylation; Sulfolobus acidocaldarius; archaea; dolichol; thermoacidophile