title: Multitask Imidazolium Salt Additives for Innovative Poly(l-lactide) Biomaterials: Morphology Control, Candida spp. Biofilm Inhibition, Human Mesenchymal Stem Cell Biocompatibility, and Skin Tolerance.
abstract: Candida species have great ability to colonize and form biofilms on medical devices, causing infections in human hosts. In this study, poly(l-lactide) films with different imidazolium salt (1-n-hexadecyl-3-methylimidazolium chloride (C16MImCl) and 1-n-hexadecyl-3-methylimidazolium methanesulfonate (C16MImMeS)) contents were prepared, using the solvent casting process. Poly(l-lactide)-imidazolium salt films were obtained with different surface morphologies (spherical and directional), and the presence of the imidazolium salt in the surface was confirmed. These films with different concentrations of the imidazolium salts C16MImCl and C16MImMeS presented antibiofilm activity against isolates of Candida tropicalis, Candida parapsilosis, and Candida albicans. The minor antibiofilm concentration assay enabled one to determine that an increasing imidazolium salt content promoted, in general, an increase in the inhibition percentage of biofilm formation. Scanning electron microscopy micrographs confirmed the effective prevention of biofilm formation on the imidazolium salt containing biomaterials. Lower concentrations of the imidazolium salts showed no cytotoxicity, and the poly(l-lactide)-imidazolium salt films presented good cell adhesion and proliferation percentages with human mesenchymal stem cells. Furthermore, no acute microscopic lesions were identified in the histopathological evaluation after contact between the films and pig ear skin. In combination with the good morphological, physicochemical, and mechanical properties, these poly(l-lactide)-based materials with imidazolium salt additives can be considered as promising biomaterials for use in the manufacturing of medical devices.
authors.forename: Clarissa M L
authors.initials: CM
authors.lastname: Schrekker
authors.forename: Yuri C A
authors.initials: YC
authors.lastname: Sokolovicz
authors.affiliation: Institute of Polymers, Composites and Biomaterials, National Research Council of Italy , Naples, Italy.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Raucci
authors.forename: Balaji S
authors.initials: BS
authors.lastname: Selukar
authors.forename: Joice S
authors.initials: JS
authors.lastname: Klitzke
authors.forename: William
authors.initials: W
authors.lastname: Lopes
authors.forename: Claudio A M
authors.initials: CA
authors.lastname: Leal
authors.forename: Igor O P
authors.initials: IO
authors.lastname: de Souza
authors.forename: Griselda B
authors.initials: GB
authors.lastname: Galland
authors.forename: João Henrique Z
authors.initials: JH
authors.lastname: Dos Santos
authors.forename: Raquel S
authors.initials: RS
authors.lastname: Mauler
authors.affiliation: School of Chemistry, Tel Aviv University , Tel Aviv, Israel.
authors.forename: Moshe
authors.initials: M
authors.lastname: Kol
authors.affiliation: Laboratoire DECOMET, Institut de Chimie de Strasbourg, CNRS-Université de Strasbourg , Strasbourg, France.
authors.forename: Samuel
authors.initials: S
authors.lastname: Dagorne
authors.forename: Luigi
authors.initials: L
authors.lastname: Ambrosio
authors.affiliation: Laboratory of Biochemistry and Toxicology, Instituto Federal Catarinense , Concórdia, SC, Brazil.
authors.forename: Mário L
authors.initials: ML
authors.lastname: Teixeira
authors.forename: Jonder
authors.initials: J
authors.lastname: Morais
authors.affiliation: Institute of Physics "Gleb Wataghin", Universidade Estadual de Campinas-UNICAMP , Campinas, SP, Brazil.
authors.forename: Richard
authors.initials: R
authors.lastname: Landers
authors.forename: Alexandre M
authors.initials: AM
authors.lastname: Fuentefria
authors.forename: Henri S
authors.initials: HS
authors.lastname: Schrekker
mesh_terms: D000818:Animals; D000935:Antifungal Agents; D001672:Biocompatible Materials; D018441:Biofilms; D002175:Candida; D006801:Humans; D059630:Mesenchymal Stem Cells; D011091:Polyesters; D012867:Skin; D013552:Swine
chemical_list: D000935:Antifungal Agents; D001672:Biocompatible Materials; D011091:Polyesters; C033616:poly(lactide)
keywords: Candida albicans; Candida parapsilosis; Candida tropicalis; antibiofilm biomaterial; human mesenchymal stem cell biocompatibility; imidazolium salt-ionic liquid; polylactic acid/poly(l-lactide)