title: [A murine model of local allergic rhinitis].
abstract: OBJECTIVE
  To establish the murine models of local allergic rhinitis (LAR) and allergic rhinitis (AR) by using ovalbumin (OVA), and to investigate the relationship between them.
  
  
  METHODS
  Thirty BALB/c mice were divided into 5 groups, (1) the nasally sensitized group (group A1) that was challenged with OVA by a 10 d procedure, (2) the control group of A1 that was challenged with phosphate-buffered saline (PBS), (3) the nasally sensitized group (group A2) that was challenged with OVA by a 25 d procedure, (4)the control group of A2 that was challenged with PBS, (5) the intraperitoneally sensitized group (group B) .The numbers of sneezing after final challenge were counted, and the serum OVA-specific immunoglobulin E (OVA-sIgE), interleukin (IL) -4, IL-13, IL-5 levels in nasal lavage fluid were measured by ELISA. Hematoxylin-eosin staining was performed to evaluate the histological change of nose and lung tissues. Graph Pad Prism 6 software was used to analyze the data.
  
  
  RESULTS
  Nasally sensitized group A1 displayed LAR symptoms of sneezing and eosinophilic infiltrating, but without increased OVA-sIgE in serum on day 10 compared with the control group of A1(t=0.697, P>0.05), OVA-sIgE in serum of group A2(2.710±1.406)ng/ml reached to statistical significance and with airway remodeling on day 25 compared with the control group of A2((0.221±0.080)ng/ml, t=4.329, P<0.05). IL-5 and IL-13 in nasal fluid showed a significant increase in the nasally sensitized group A1, compared with the group A2(t values were 2.442, 2.804, P values were less then 0.05).
  
  
  CONCLUSIONS
  A short time intranasal instillation with OVA could establish LAR murine model, continuing OVA challenge could increase serum sIgE level and with airway remodeling. LAR mice show a unique characteristic by expressing higher IL-5 and IL-13 in nose than AR mice, but sIgE in serum remains at a normal level.
authors.affiliation: Guangxi University of Traditional Chinese Medicine, Nanning 530001, China; Department of Otorhinolaryngology, the People's Hospital of Guangxi Zhuang Autonmous Region, Nanning 530021, China.
authors.forename: B W
authors.initials: BW
authors.lastname: Chen
authors.affiliation: Department of Otorhinolaryngology, the People's Hospital of Guangxi Zhuang Autonmous Region, Nanning 530021, China.
authors.forename: S H
authors.initials: SH
authors.lastname: Qu
authors.affiliation: Department of Otorhinolaryngology, the People's Hospital of Guangxi Zhuang Autonmous Region, Nanning 530021, China.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology, the People's Hospital of Guangxi Zhuang Autonmous Region, Nanning 530021, China.
authors.forename: L S
authors.initials: LS
authors.lastname: Ye
authors.affiliation: Department of Otorhinolaryngology, the People's Hospital of Guangxi Zhuang Autonmous Region, Nanning 530021, China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Zhang
authors.affiliation: Guangxi University of Traditional Chinese Medicine, Nanning 530001, China; Department of Otorhinolaryngology, the People's Hospital of Guangxi Zhuang Autonmous Region, Nanning 530021, China.
authors.forename: T J
authors.initials: TJ
authors.lastname: Qin
authors.affiliation: Guangxi University of Traditional Chinese Medicine, Nanning 530001, China; Department of Otorhinolaryngology, the People's Hospital of Guangxi Zhuang Autonmous Region, Nanning 530021, China.
authors.forename: H
authors.initials: H
authors.lastname: Fan
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils; D007073:Immunoglobulin E / Q000097:blood; D018793:Interleukin-13 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D015848:Interleukin-5 / Q000032:analysis; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009666:Nose / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D065631:Rhinitis, Allergic / Q000097:blood / Q000209:etiology*; D012912:Sneezing; D012965:Sodium Chloride
chemical_list: C508598:IL4 protein, human; D018793:Interleukin-13; D015848:Interleukin-5; D015847:Interleukin-4; D007073:Immunoglobulin E; D012965:Sodium Chloride; D010047:Ovalbumin