title: Obesity decreases the oxidant stress induced by tobacco smoke in a rat model.
abstract: Obesity and emphysema are associated with low-grade systemic inflammation and oxidant stress. Assuming that the oxidant stress induced by emphysema would be decreased by obesity, we analyzed the oxidant/antioxidant state in a rat model combining both diseases simultaneously. Obesity was induced using sucrose, while emphysema by exposure to tobacco smoke. End-points evaluated were: body weight, abdominal fat, plasma dyslipidemia and malondialdehyde (MDA), insulin and glucose AUC, activities of Mn-superoxide dismutase (Mn-SOD), glutathione reductase (GR), glutathione transferase (GST) and glutathione peroxidase (GPx); lung MnSOD and 3-nitrotyrosine (3-NT) immunostaining, and expression of αV and β6 integrin subunits. In rats with obesity, the body weight, abdominal fat, plasma triglyceride levels, glucose AUC, insulin levels, GST activity, and αV and β6 integrin expressions were amplified. The rats with emphysema had lower values of body weight, abdominal fat, plasma insulin, triglycerides and glucose AUC but higher values of plasma MDA, GPx activity, and the lung expression of the αV and β6 integrins. The combination of obesity and emphysema compared to either condition alone led to diminished body weight, abdominal fat, plasma insulin MDA levels, GPx and GST activities, and αV and β6 integrin expressions; these parameters were all previously increased by obesity. Immunostaining for MnSOD augmented in all experimental groups, but the staining for 3-NT only increased in rats treated with tobacco alone or combined with sucrose. Results showed that obesity reduces oxidant stress and integrin expression, increasing antioxidant enzyme activities; these changes seem to partly contribute to a protective mechanism of obesity against emphysema development.
authors.affiliation: a Departamento de Investigación en Fibrosis Pulmonar , Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas , México , Mexico.
authors.forename: Martha
authors.initials: M
authors.lastname: Montaño
authors.affiliation: b Departamento de Sistemas Biológicos , Universidad Autónoma Metropolitana-Xochimilco , México , Mexico.
authors.forename: J
authors.initials: J
authors.lastname: Pérez-Ramos
authors.affiliation: b Departamento de Sistemas Biológicos , Universidad Autónoma Metropolitana-Xochimilco , México , Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Esquivel
authors.affiliation: c Departamento de Patología , Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas , México , Mexico.
authors.forename: R
authors.initials: R
authors.lastname: Rivera-Rosales
authors.affiliation: d Departamento de Enfermedades Crónico Degenerativas , Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas (INER) , México , Mexico.
authors.forename: G
authors.initials: G
authors.lastname: González-Avila
authors.affiliation: a Departamento de Investigación en Fibrosis Pulmonar , Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas , México , Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Becerril
authors.affiliation: a Departamento de Investigación en Fibrosis Pulmonar , Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas , México , Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Checa
authors.affiliation: a Departamento de Investigación en Fibrosis Pulmonar , Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas , México , Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Ramos
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000032:analysis; D004646:Emphysema / Q000139:chemically induced / Q000378:metabolism*; D005951:Glucose Tolerance Test; D008054:Lipid Peroxides / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009765:Obesity / Q000150:complications / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D017208:Rats, Wistar; D012906:Smoke / Q000009:adverse effects*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D008054:Lipid Peroxides; D012906:Smoke; D014028:Tobacco Smoke Pollution
keywords: Emphysema; glutathione peroxidase; malondialdehyde; obesity; oxidant stress