title: Hydrogen peroxide detection with high specificity in living cells and inflamed tissues.
abstract: Hydrogen peroxide (H2O2) detection in biological systems is of significant importance, which act as critical second messenger in fundamental biological processes. Here, we report on a chemoselective fluorescent naphthylimide peroxide probe (NPP) for the H2O2 detection in vitro and in vivo. NPP is a phenylboronic acid-caged chromophore that selectively responds to H2O2 through a self-immolate mechanism. NPP exhibited high sensitivity and selectivity to H2O2 with distinctive fluorescence change due to the excellent two-photon excitation property, which permits the facile detection of inflammation produced H2O2 and offers chance to monitor the inflammatory stages in diseased cells.
authors.affiliation: Department of Chemistry, Key Laboratory of Biomedical Polymers of Ministry of Education; State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Rong
authors.affiliation: Department of Chemistry, Key Laboratory of Biomedical Polymers of Ministry of Education.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Key Laboratory of Biomedical Polymers of Ministry of Education.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, P. R. China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Hu
authors.affiliation: Department of Chemistry, Key Laboratory of Biomedical Polymers of Ministry of Education.
authors.forename: Jun
authors.initials: J
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, P. R. China.
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Shu
authors.affiliation: Department of Chemistry, Key Laboratory of Biomedical Polymers of Ministry of Education.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Key Laboratory of Biomedical Polymers of Ministry of Education.
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
keywords: fluorescence imaging; hydrogen peroxide; inflammation