title: Cytogenetics in the management of Philadelphia-negative myeloproliferative neoplasms: an update by the Groupe francophone de cytogénétique hématologique (GFCH).
abstract: The recent years have witnessed tremendous progress in the molecular characterization of Philadelphia-negative myeloproliferative neoplasms (MPN). Beside a better understanding of pathophysiology, these abnormalities often constitute very useful diagnostic markers in diseases where exclusion of reactive states used to be the strongest argument. However, conventional and molecular cytogenetics keep a major interest in MPN, either as a second line exploration, in cases where no molecular marker is available, for differential diagnosis or as a proof of clonality or in first line for cases with hyperleukocytosis, for differential diagnosis (CML), to evidence druggable targets (ABL1, RET, PDGFR…) or as a prognosis marker. In this article, we will review the interest of cytogenetic techniques in myeloproliferative neoplasms.
authors.affiliation: Laboratoire d'hématologie, Unité de cytogénétique onco-hématologique, Hôpital Saint-Antoine, AP-HP, Paris, France.
authors.forename: Chrystèle
authors.initials: C
authors.lastname: Bilhou-Nabéra
authors.affiliation: Laboratoire d'hématologie-cytogénétique, Hôpital Haut-Lévêque, CHU de Bordeaux, Pessac, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Bidet
authors.affiliation: Laboratoire d'hématologie et de cytogénétique, Hôpital Avicenne, AP-HP, Bobigny, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Eclache
authors.affiliation: Laboratoire d'hématologie biologique, CHRU de Brest & Equipe Ecla, Inserm U1078, Université de Bretagne occidentale, Brest, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Lippert
authors.affiliation: Laboratoire de cytogénétique et biologie moléculaire, Institut Paoli-Calmettes, Marseille, France.
authors.forename: Marie-Joëlle
authors.initials: MJ
authors.lastname: Mozziconacci
mesh_terms: D020732:Cytogenetic Analysis / Q000379:methods / Q000592:standards* / Q000639:trends; D006405:Hematology / Q000458:organization & administration / Q000592:standards*; D006801:Humans; D054438:Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative / Q000175:diagnosis* / Q000235:genetics; D009196:Myeloproliferative Disorders / Q000175:diagnosis* / Q000235:genetics; D010677:Philadelphia Chromosome; D012955:Societies, Medical / Q000592:standards
keywords: FISH; cytogenetic abnormalities; philadelphia-negatives myeloproliferative neoplasms