title: Structural basis for the roles of starch and sucrose in homo-exopolysaccharide formation by Lactobacillus reuteri 35-5.
abstract: Lactic acid bacteria (LAB) produce exopolysaccharides (EPS) that are important for biofilm formation in the mammalian oral cavity and gastrointestinal tract. Sucrose is a well-known substrate for homo-EPS formation by Lactobacillus reuteri glucansucrases (GS). Starch is the main fermentable carbohydrate in the human diet, and often consumed simultaneously with sucrose. Recently we have characterized L. reuteri strains that also possess 4,6-α-glucanotransferases (4,6-α-GTases) that act on starch yielding isomalto-/malto-polysaccharides. In this study we have characterized the EPS formed by L. reuteri 35-5 cells and enzymes from sucrose plus starch. The data show that both in vivo and in vitro the L. reuteri 35-5 GS and 4,6-α-GTase enzymes, incubated with sucrose plus starch, cross-react and contribute to synthesis of the final hybrid EPS products. This may have strong effects on the EPS functional properties, influence biofilm formation, and affect the relationship between dietary intake of sucrose and starch, and dental caries formation.
authors.affiliation: Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands; The State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands.
authors.forename: Justyna M
authors.initials: JM
authors.lastname: Dobruchowska
authors.affiliation: Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands.
authors.forename: Rachel M
authors.initials: RM
authors.lastname: van der Kaaij
authors.affiliation: Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands; NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, NL-3584 CH Utrecht, The Netherlands.
authors.forename: Gerrit J
authors.initials: GJ
authors.lastname: Gerwig
authors.affiliation: Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands. Electronic address: L.Dijkhuizen@rug.nl.
authors.forename: Lubbert
authors.initials: L
authors.lastname: Dijkhuizen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001704:Biopolymers / Q000096:biosynthesis*; D006004:Glycogen Debranching Enzyme System / Q000235:genetics / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism; D052200:Limosilactobacillus reuteri / Q000235:genetics / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis*; D013213:Starch / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001704:Biopolymers; D006004:Glycogen Debranching Enzyme System; D011134:Polysaccharides; D013395:Sucrose; D013213:Starch; D016695:Glycosyltransferases; C033837:alternansucrase; C020569:4 alpha-glucanotransferase
keywords: 4,6-α-glucanotransferase; Exopolysaccharides; Glucansucrase; Lactobacillus reuteri 35-5; Starch; Sucrose