title: Generation of a Mouse Full-length Balancer with Versatile Cassette-shuttling Selection Strategy.
abstract: Balancer chromosomes are important tools for a variety of genetic manipulations in lower model organisms, owing to their ability to suppress recombination. In mouse, however, such effort has not been accomplished, mostly due to the size of the chromosomes and the complexity of multiple step chromosomal engineering. We developed an effective and versatile cassette-shuttling selection (CASS) strategy involving only two selection markers to achieve the sequential production of multiple large inversions along the chromosome. Using this strategy, we successfully generated the first full-length balancer in mice and showed that Balancer 17M-GFP can efficiently suppress recombination. Our study has not only generated a useful genetic resource, but also provided a strategy for constructing mammalian balancer chromosomes.
authors.affiliation: 1. State Key Laboratory of Genetic Engineering and National Center for International Research of Development and Disease, Collaborative Innovation Center for Genetics and Development, Fudan-Yale Biomedical Research Center, Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai 200433, China.
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Ye
authors.affiliation: 1. State Key Laboratory of Genetic Engineering and National Center for International Research of Development and Disease, Collaborative Innovation Center for Genetics and Development, Fudan-Yale Biomedical Research Center, Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai 200433, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: 1. State Key Laboratory of Genetic Engineering and National Center for International Research of Development and Disease, Collaborative Innovation Center for Genetics and Development, Fudan-Yale Biomedical Research Center, Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai 200433, China.
authors.forename: Rongbo
authors.initials: R
authors.lastname: Li
authors.affiliation: 1. State Key Laboratory of Genetic Engineering and National Center for International Research of Development and Disease, Collaborative Innovation Center for Genetics and Development, Fudan-Yale Biomedical Research Center, Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai 200433, China.; 2. Howard Hughes Medical Institute, Department of Molecular, Cellular, Developmental Biology, University of Colorado at Boulder, Boulder, CO 80309, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Han
authors.affiliation: 1. State Key Laboratory of Genetic Engineering and National Center for International Research of Development and Disease, Collaborative Innovation Center for Genetics and Development, Fudan-Yale Biomedical Research Center, Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai 200433, China.; 3. Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: 1. State Key Laboratory of Genetic Engineering and National Center for International Research of Development and Disease, Collaborative Innovation Center for Genetics and Development, Fudan-Yale Biomedical Research Center, Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai 200433, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wu
authors.affiliation: 1. State Key Laboratory of Genetic Engineering and National Center for International Research of Development and Disease, Collaborative Innovation Center for Genetics and Development, Fudan-Yale Biomedical Research Center, Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai 200433, China.; 4. Howard Hughes Medical Institute, Department of Genetics, Yale University School of Medicine, New Haven, CT 06536, USA.
authors.forename: Tian
authors.initials: T
authors.lastname: Xu
mesh_terms: D000818:Animals; D002460:Cell Line; D007446:Chromosome Inversion / Q000235:genetics; D002875:Chromosomes / Q000235:genetics*; D005260:Female; D005818:Genetic Engineering / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011995:Recombination, Genetic / Q000235:genetics*
keywords: 17M-GFP.; Mouse full-length balancer chromosome; cassette-shuttling selection (CASS) strategy