title: Microarrays/DNA Chips for the Detection of Waterborne Pathogens.
abstract: DNA microarrays are useful for the simultaneous detection of microorganisms in water samples. Specific probes targeting waterborne pathogens are selected with bioinformatics tools, synthesized and spotted onto a DNA array. Here, the construction of a DNA chip for waterborne pathogen detection is described, including the processes of probe in silico selection, synthesis, validation, and data analysis.
authors.affiliation: Host-Pathogen Unit, Research Institute for Medicine (iMed-ULisboa), Faculdade de Farmácia da Universidade de Lisboa, Universidade de Lisboa, Lisbon, Portugal. f.vale@ff.ul.pt.|Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Universidade de Lisboa, Lisbon, Portugal. f.vale@ff.ul.pt.
authors.forename: Filipa F
authors.initials: FF
authors.lastname: Vale
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D019295:Computational Biology; D004269:DNA, Bacterial / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial
keywords: Bacterial detection; DNA chip; DNA probe; Indicator bacteria; Microarray; Microbial detection; Molecular method; Water quality; Waterborne detection; Waterborne pathogen