title: Measurement of Inositol Triphosphate Levels from Rat Hippocampal Slices.
abstract: Inositol triphosphate (IP3) is an important second messenger that participates in signal transduction pathways in diverse cell types including hippocampal neurons. Stimulation of phospholipase C in response to various stimuli (hormones, growth factors, neurotransmitters, neurotrophins, neuromodulators, odorants, light,etc) results in hydrolysis of phosphatidylinositol 4, 5-bisphosphate (PIP2), a phospholipid that is located in the plasma membrane, and leads to the production of IP3and diacylglycerol. Binding of IP3to the IP3receptor (IP3R) induces Ca2+release from intracellular stores and enables the initiation of intracellular Ca2+-dependent signaling. Here we describe a procedure for the measurement of cellular IP3levels in tissue homogenates prepared from rat hippocampal slices.
authors.affiliation: Department of Neurobiology, Northwestern University, Evanston, USA.
authors.forename: Nino
authors.initials: N
authors.lastname: Tabatadze
authors.affiliation: Department of Neurobiology, Northwestern University, Evanston, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Woolley