title: Rapid separation of the major phospholipid classes on a single aminopropyl cartridge.
abstract: A rapid method for the separation of the individual phospholipid classes phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylinositol (PI) by a single solid-phase extraction was developed. PC, PE, PS and PI were sequentially eluted from aminopropyl bonded silica with acetonitrile/n-propanol (2∶1, vol/vol), methanol, isopropanol/methanolic HCl (4∶1, vol/vol) and methanol/methanolic HCl (9∶1, vol/vol). Standard recoveries were over 95% for PC and PE and over 85% for PS and PI with undistorted fatty acid composition. The separation of complex lipid mixtures on aminopropyl minicolumns can be refined to the level of individual phospholipid classes.
authors.affiliation: Institute for Prophylaxis of Cardiovascular Disease, University of Munich, D-80336 Munich, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Pietsch
authors.forename: R L
authors.initials: RL
authors.lastname: Lorenz
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D010713:Phosphatidylcholines / Q000032:analysis / Q000145:classification; D010714:Phosphatidylethanolamines / Q000032:analysis / Q000145:classification; D010716:Phosphatidylinositols / Q000032:analysis / Q000145:classification; D010718:Phosphatidylserines / Q000032:analysis / Q000145:classification; D010743:Phospholipids / Q000032:analysis* / Q000145:classification; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D010743:Phospholipids; C483858:phosphatidylethanolamine