title: Lysine acetylation in mitochondria: From inventory to function.
abstract: Cellular signaling pathways are regulated in a highly dynamic fashion in order to quickly adapt to distinct environmental conditions. Acetylation of lysine residues represents a central process that orchestrates cellular metabolism and signaling. In mitochondria, acetylation seems to be the most prevalent post-translational modification, presumably linked to the compartmentation and high turnover of acetyl-CoA in this organelle. Similarly, the elevated pH and the higher concentration of metabolites in mitochondria seem to favor non-enzymatic lysine modifications, as well as other acylations. Hence, elucidating the mechanisms for metabolic control of protein acetylation is crucial for our understanding of cellular processes. Recent advances in mass spectrometry-based proteomics have considerably increased our knowledge of the regulatory scope of acetylation. Here, we review the current knowledge and functional impact of mitochondrial protein acetylation across species. We first cover the experimental approaches to identify and analyze lysine acetylation on a global scale, we then explore both commonalities and specific differences of plant and animal acetylomes and the evolutionary conservation of protein acetylation, as well as its particular impact on metabolism and diseases. Important future directions and technical challenges are discussed, and it is pointed out that the transfer of knowledge between species and diseases, both in technology and biology, is of particular importance for further advancements in this field.
authors.affiliation: Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Am Klopferspitz 18, DE-82152 Martinsried, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Hosp
authors.affiliation: Plant Proteomics, Max Planck Institute for Plant Breeding Research, Carl von Linné Weg 10, DE-50829 Cologne, Germany.
authors.forename: Ines
authors.initials: I
authors.lastname: Lassowskat
authors.affiliation: Therapeutic Research Groups/Onc II, Bayer Pharma AG, Muellerstrasse 178, DE-13353 Berlin, Germany.
authors.forename: Valeria
authors.initials: V
authors.lastname: Santoro
authors.affiliation: Bayer CropScience NV, Technologiepark 38, BL-9052 Gent (Zwijnaarde), Belgium.
authors.forename: David
authors.initials: D
authors.lastname: De Vleesschauwer
authors.affiliation: Bayer Pharma AG, Department Heart and Lung Diseases Research, Aprather Weg 18a, DE-42113 Wuppertal, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fliegner
authors.affiliation: Bayer CropScience NV, Technologiepark 38, BL-9052 Gent (Zwijnaarde), Belgium.
authors.forename: Henning
authors.initials: H
authors.lastname: Redestig
authors.affiliation: Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Am Klopferspitz 18, DE-82152 Martinsried, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Mann
authors.affiliation: Therapeutic Research Groups/Onc II, Bayer Pharma AG, Muellerstrasse 178, DE-13353 Berlin, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Christian
authors.affiliation: Bayer CropScience NV, Technologiepark 38, BL-9052 Gent (Zwijnaarde), Belgium.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Hannah
authors.affiliation: Plant Proteomics, Max Planck Institute for Plant Breeding Research, Carl von Linné Weg 10, DE-50829 Cologne, Germany; Plant Physiology, Institute of Plant Biology and Biotechnology, University of Muenster, Schlossplatz 7, DE-48149 Muenster, Germany. Electronic address: iris.finkemeier@uni-muenster.de.
authors.forename: Iris
authors.initials: I
authors.lastname: Finkemeier
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000107:Acetylation; D000818:Animals; D019295:Computational Biology; D008239:Lysine / Q000378:metabolism*; D013058:Mass Spectrometry; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D010944:Plants; D011499:Protein Processing, Post-Translational; D040901:Proteomics
chemical_list: D024101:Mitochondrial Proteins; D000105:Acetyl Coenzyme A; D008239:Lysine
keywords: ATP synthase; Acetyl-CoA; Arabidopsis; Human; Lysine acetylation; Mitochondria; Mouse; Respiration; Rice; TCA cycle