title: Legionella saoudiensis sp. nov., isolated from a sewage water sample.
abstract: A Gram-stain-negative, bacilli-shaped bacterial strain, LS-1T, was isolated from a sewage water sample collected in Jeddah, Saudi Arabia. The taxonomic position of strain LS-1T was investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences and those of four other genes indicated that strain LS-1T belongs to the genus Legionella in the family Legionellaceae. Regarding the 16S rRNA gene, the most closely related species are Legionella rowbothamii LLAP-6T (98.6 %) and Legionella lytica L2T (98.5 %). The mip gene sequence of strain LS-1T showed 94 % sequence similarity with that of L. lytica L2T and 93 % similarity with that of L. rowbothamii LLAP-6T. Strain LS-1T grew optimally at a temperature of 32 °C on a buffered charcoal yeast extract (BCYE) agar plate in a 5 % CO2 atmosphere and had a flagellum. The combined phylogenetic, phenotypic and genomic sequence data suggest that strain LS-1T represents a novel species of the genus Legionella, for which the name Legionella saoudiensis sp. nov. is proposed. The type strain is LS-1T (=DSM 101682T=CSUR P2101T).
authors.affiliation: Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.|Unité des Rickettsies, URMITE UMR CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, IHU Méditerranée Infection, Aix-Marseille Université, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
authors.forename: Leena Hussein
authors.initials: LH
authors.lastname: Bajrai
authors.affiliation: Department of Medical Laboratory Technology, Special Infectious Agents Unit, King Fahd Medical Research Center, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Esam Ibraheem
authors.initials: EI
authors.lastname: Azhar
authors.affiliation: Department of Medical Laboratory Technology, Special Infectious Agents Unit, King Fahd Medical Research Center, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Yasir
authors.affiliation: Unité des Rickettsies, URMITE UMR CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, IHU Méditerranée Infection, Aix-Marseille Université, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Jardot
authors.affiliation: Unité des Rickettsies, URMITE UMR CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, IHU Méditerranée Infection, Aix-Marseille Université, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
authors.forename: Lina
authors.initials: L
authors.lastname: Barrassi
authors.affiliation: Unité des Rickettsies, URMITE UMR CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, IHU Méditerranée Infection, Aix-Marseille Université, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.affiliation: Unité des Rickettsies, URMITE UMR CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, IHU Méditerranée Infection, Aix-Marseille Université, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: La Scola
authors.affiliation: Unité des Rickettsies, URMITE UMR CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, IHU Méditerranée Infection, Aix-Marseille Université, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Pagnier
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D007875:Legionella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012529:Saudi Arabia; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage