title: Biokinetics of13C in the human body after oral administration of13C-labeled glucose as an index for the biokinetics of14C.
abstract: The retention of13C in the human body after oral administration of13C-labeled glucose was studied in three healthy volunteer subjects to estimate the 50 year cumulative body burden for13C as an index of the committed dose of the radioisotope14C. After administration of13C-labeled glucose, the volunteers ingested controlled diets with a fixed number of calories for 112 d. Samples of breath and urine were collected up to 112 d after administration. Samples of feces were collected up to 14 d after administration. Hair samples were obtained at 119 d after administration and analyzed as a representative index of the rate of excretion of organic13C via pathways such as skin cell exfoliation and mucus secretion. All samples were analyzed for13C/12C atomic ratio to determine the rate of excretion via each pathway. We then constructed a metabolic model with a total of four pathways (breath, urine, feces, and other) comprising seven compartments. We determined the values of the biokinetic parameters in the model by using the obtained excretion data. From 74% to 94% of the13C administered was excreted in breath, whereas  <2% was excreted in urine and feces. In the other pathway, the excretion rate constant in the compartment with the longest residence time stretched to hundreds of days but the rate constant for each subject was not statistically significant (P value  >  0.1). In addition, the dataset for one of the three subjects was markedly different from those of the other two. When we estimated the 50 year cumulative body burden for13C by using our model and we included non-statistically significant parameters, a considerable cumulative body burden was found in the compartments excreting to the other pathway. Although our results on the cumulative body burden of13C from orally administered carbon as glucose were inconclusive, we found that the compartments excreting to the other pathway had a markedly long residence time and therefore should be studied further to clarify the fate of carbon in the human body. In addition to excreta, data for serum and blood cell samples were also collected from the subjects to examine the metabolism of13C in human body.
authors.affiliation: Institute for Environmental Sciences, Aomori, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Masuda
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tako
authors.forename: Kensaku
authors.initials: K
authors.lastname: Matsushita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takeda
authors.forename: Masahiro
authors.initials: M
authors.lastname: Endo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Shun'ichi
authors.initials: S
authors.lastname: Hisamatsu
mesh_terms: D000284:Administration, Oral; D000328:Adult; D002247:Carbon Isotopes / Q000008:administration & dosage / Q000493:pharmacokinetics; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics*; D005947:Glucose / Q000008:administration & dosage / Q000493:pharmacokinetics*; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D011874:Radiometry
chemical_list: D002247:Carbon Isotopes; D002250:Carbon Radioisotopes; D005947:Glucose