title: Enhanced biglycan gene expression in the adipose tissues of obese women and its association with obesity-related genes and metabolic parameters.
abstract: Extracellular matrix (ECM) remodeling dynamically occurs to accommodate adipose tissue expansion during obesity. One non-fibrillar component of ECM, biglycan, is released from the matrix in response to tissue stress; the soluble form of biglycan binds to toll-like receptor 2/4 on macrophages, causing proinflammatory cytokine secretion. To investigate the pattern and regulatory properties of biglycan expression in human adipose tissues in the context of obesity and its related diseases, we recruited 21 non-diabetic obese women, 11 type 2 diabetic obese women, and 59 normal-weight women. Regardless of the presence of diabetes, obese patients had significantly higher biglycan mRNA in both visceral and subcutaneous adipose tissue. Biglycan mRNA was noticeably higher in non-adipocytes than adipocytes and significantly decreased during adipogenesis. Adipose tissue biglycan mRNA positively correlated with adiposity indices and insulin resistance parameters; however, this relationship disappeared after adjusting for BMI. In both fat depots, biglycan mRNA strongly correlated with the expression of genes related to inflammation and endoplasmic reticulum stress. In addition, culture of human preadipocytes and differentiated adipocytes under conditions mimicking the local microenvironments of obese adipose tissues significantly increased biglycan mRNA expression. Our data indicate that biglycan gene expression is increased in obese adipose tissues by altered local conditions.
authors.affiliation: Department of Physiology, Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Jimin
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Physiology, Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Seul Ki
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Department of Physiology, Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Shin
authors.affiliation: Department of Physiology, Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Un-Woo
authors.initials: UW
authors.lastname: Jeoun
authors.affiliation: Department of Physiology, Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Yeon Jin
authors.initials: YJ
authors.lastname: Jang
authors.affiliation: Department of Family Medicine, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Hye Soon
authors.initials: HS
authors.lastname: Park
authors.affiliation: Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Jong-Hyeok
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Pathology, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Gyung-Yub
authors.initials: GY
authors.lastname: Gong
authors.affiliation: Department of Plastic Surgery, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Taik Jong
authors.initials: TJ
authors.lastname: Lee
authors.affiliation: Department of Plastic Surgery, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Joon Pio
authors.initials: JP
authors.lastname: Hong
authors.affiliation: Department of Family Medicine, Inha University, College of Medicine, Incheon, Korea.
authors.forename: Yeon Ji
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of General Surgery, Inha University, College of Medicine, Incheon, Korea.
authors.forename: Yoon-Suk
authors.initials: YS
authors.lastname: Heo
mesh_terms: D050153:Abdominal Fat / Q000502:physiology; D017667:Adipocytes / Q000473:pathology / Q000502:physiology; D000273:Adipose Tissue / Q000502:physiology*; D000328:Adult; D058578:Biglycan / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D002454:Cell Differentiation / Q000235:genetics; D048429:Cell Size; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics; D059865:Endoplasmic Reticulum Stress / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D006943:Hyperglycemia / Q000235:genetics; D008875:Middle Aged; D009765:Obesity / Q000235:genetics*; D050151:Subcutaneous Fat / Q000502:physiology
chemical_list: C546884:BGN protein, human; D058578:Biglycan