title: Evaluation of adiponectin profile in Italian patients affected by obstructive sleep apnea syndrome.
abstract: Obstructive sleep apnea syndrome (OSAS) is a common breathing disorder; obesity represents a major risk factor for the development and progression of OSAS although obese patients do not necessarily suffer from OSAS. Adipose tissue communicates with lung in both physiologic and pathologic conditions through the production of adipokines, hormones active in metabolic and inflammatory processes. To explore the extent to which Acrp30 impacts on pathophysiology of OSAS and whether these proteins could be considered as targets for both diagnosis and therapy through enzyme-linked immunosorbent assay, western blotting analysis and fast protein liquid chromatography we have analyzed total levels as well as oligomer distribution in OSAS patients. Our data demonstrated that total Acrp30 serum levels were statistically reduced in OSAS patients compared to controls (p value = 0.02). Within a selected subgroup of OSAS patients with a BMI<30, Acrp30 levels were still statistically lower in OSAS than in control group (p value < 0.05). In addition, more severe patients (AHI>15) exhibited a more pronounced reduction of Acrp30 levels. Interestingly, this reduction is mainly due to a specific decrease of the HMW oligomers, those exhibiting major biological significance. In conclusion, our results strongly suggest a role for Acrp30 oligomerization in OSAS; in fact, the down-regulation of Acpr30 and its HMW oligomers seems to be correlated to illness status independently of concomitant presence of obesity. In addition, this reduction is mainly due to the high-weight oligomers of Acrp30 suggesting a functional role of this adipokine in OSAS.
authors.affiliation: Dipartimento di Scienze Mediche e Chirurgiche, Instituto di Malattie Respiratorie, Università di Foggia, Via S. Pinto 1, 71121, Foggia, Italy.
authors.forename: Donato
authors.initials: D
authors.lastname: Lacedonia
authors.affiliation: CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145, Napoli, Italy.
authors.forename: Ersilia
authors.initials: E
authors.lastname: Nigro
authors.affiliation: Dipartimento di Medicina Sperimentale, Seconda Università degli Studi di Napoli, Via L. Bianchi, 80131, Napoli, Italy. Electronic address: mariagabriella.matera@unina2.it.
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Matera
authors.affiliation: CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145, Napoli, Italy; Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, 80131, Napoli, Italy.
authors.forename: Olga
authors.initials: O
authors.lastname: Scudiero
authors.affiliation: CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145, Napoli, Italy.
authors.forename: Maria Ludovica
authors.initials: ML
authors.lastname: Monaco
authors.affiliation: CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145, Napoli, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Polito
authors.affiliation: Dipartimento di Scienze Mediche e Chirurgiche, Instituto di Malattie Respiratorie, Università di Foggia, Via S. Pinto 1, 71121, Foggia, Italy.
authors.forename: Giovanna Elisiana
authors.initials: GE
authors.lastname: Carpagnano
authors.affiliation: Dipartimento di Scienze Mediche e Chirurgiche, Instituto di Malattie Respiratorie, Università di Foggia, Via S. Pinto 1, 71121, Foggia, Italy.
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Foschino Barbaro
authors.affiliation: Dipartimento di Scienze Cardio-Toraciche e Respiratorie, Seconda Università degli Studi di Napoli, Via L. Bianchi, 80131, Napoli, Italy.
authors.forename: Gennaro
authors.initials: G
authors.lastname: Mazzarella
authors.affiliation: Dipartimento di Scienze Cardio-Toraciche e Respiratorie, Seconda Università degli Studi di Napoli, Via L. Bianchi, 80131, Napoli, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Bianco
authors.affiliation: Dipartimento di Scienze Mediche e Chirurgiche, Instituto di Malattie Respiratorie, Università di Foggia, Via S. Pinto 1, 71121, Foggia, Italy; Dipartimento di Scienze e Tecnologie Ambientali Biologiche Farmaceutiche, Seconda Università degli Studi di Napoli, Via G. Vivaldi 42, 81100, Caserta, Italy.
authors.forename: Aurora
authors.initials: A
authors.lastname: Daniele
mesh_terms: D052242:Adiponectin / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D015992:Body Mass Index; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007558:Italy; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009765:Obesity / Q000150:complications*; D012720:Severity of Illness Index; D020181:Sleep Apnea, Obstructive / Q000209:etiology / Q000503:physiopathology*
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin
keywords: Adiponectin; Lung; Obesity; Obstructive sleep apnea