title: Identification and characterization of novel anticoagulant peptide with thrombolytic effect and nutrient oligopeptides with high branched chain amino acid from Whitmania pigra protein.
abstract: Natural and nutrient substances for cardiovascular disease are promising and capture researchers' minds. Two kinds of novel bioactive peptides (high Fischer's ratio oligopeptides and anticoagulant peptides) were obtained from Whitmania pigra protein via enzymatic hydrolysis. An oligopeptide (MW<874.0 Da) named as HF2 was obtained via chromatography purification procedures with a high Fischer's ratio of 31.92 ± 1.36 and low phenylalanine + tyrosine content of 0.98 ± 0.04 %. Another peptide (WA3-1), prepared by alcalase AF 2.4 L-catalyzed hydrolysis and then purified by DEAE Sepharose FF, gel Sephadex G-15 chromatography, exhibited high anticoagulant activity with prolonging significantly plasma clotting time on activated partial thromboplastin time, prothrombin time, thrombin time (p < 0.01) and powerful thrombolytic activity. Amino acid composition and MALDI-TOF/TOF MS analysis showed that WA3-1 contained 11 amino acids (MW: 1422.0 Da) with the sequence as NH2-His-Asp-Phe-Leu-Asn-Asn-Lys-Leu-Glu-Tyr-Glu-COOH. Abundant negatively charged amino acids in C-terminal, as well as the special residue Lys contribute to its anticoagulant capacity. This research provided a novel natural candidate for the manufacture of nutrient oligopeptides with high branched chain amino acid, and anticoagulant thrombolytic agent in pharmaceutical industry with helping prevent from thrombosis and related cardiovascular diseases.
authors.affiliation: College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu, 610065, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Wushan Road 381, Guangzhou, 510641, China.
authors.forename: Yijing
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Wushan Road 381, Guangzhou, 510641, China.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Wu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Wushan Road 381, Guangzhou, 510641, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Wushan Road 381, Guangzhou, 510641, China. huagongchuanda@yahoo.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Wushan Road 381, Guangzhou, 510641, China. xflibio@scut.edu.cn.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000597:Amino Acids, Branched-Chain; D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects*; D006801:Humans; D007865:Leeches / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D015912:Thrombolytic Therapy
chemical_list: D000597:Amino Acids, Branched-Chain; D000925:Anticoagulants; D009842:Oligopeptides
keywords: Amino acid composition; Anticoagulant activity; Oligopeptide; Thrombolytic effect; Whitmania pigra