title: The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models.
abstract: Targeted genome editing technology has been widely used in biomedical studies. The CRISPR-associated RNA-guided endonuclease Cas9 has become a versatile genome editing tool. The CRISPR/Cas9 system is useful for studying gene function through efficient knock-out, knock-in or chromatin modification of the targeted gene loci in various cell types and organisms. It can be applied in a number of fields, such as genetic breeding, disease treatment and gene functional investigation. In this review, we introduce the most recent developments and applications, the challenges, and future directions of Cas9 in generating disease animal model. Derived from the CRISPR adaptive immune system of bacteria, the development trend of Cas9 will inevitably fuel the vital applications from basic research to biotechnology and bio-medicine.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650500, China;Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Shao
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650500, China. xtrgfq@hotmail.com.
authors.forename: Tian-Rui
authors.initials: TR
authors.lastname: Xu
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China. chenc@mail.kiz.ac.cn.
authors.forename: Ce-Shi
authors.initials: CS
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D064113:CRISPR-Cas Systems / Q000235:genetics*; D004195:Disease Models, Animal; D000072669:Gene Editing / Q000379:methods*; D015316:Genetic Therapy; D023281:Genomics; D006801:Humans
keywords: Animal models; CRISPR/Cas9; Gene therapy