title: Fisetin Protects DNA Against Oxidative Damage and Its Possible Mechanism.
abstract: OBJECTIVE
  The paper tries to assess the protective effect of fisetin against •OH-induced DNA damage, then to investigate the possible mechanism.
  
  
  METHODS
  The protective effect was evaluated based on the content of malondialdehyde (MDA). The possible mechanism was analyzed using various antioxidant methods in vitro, including •OH scavenging (deoxyribose degradation), •O2 (-) scavenging (pyrogallol autoxidation), DPPH• scavenging, ABTS•(+) scavenging, and Cu(2+)-reducing power assays.
  
  
  RESULTS
  Fisetin increased dose-dependently its protective percentages against •OH-induced DNA damage (IC50 value =1535.00±29.60 µM). It also increased its radical-scavenging percentages in a dose-dependent manner in various antioxidants assays. Its IC50 values in •OH scavenging, •O2(-) scavenging, DPPH• scavenging, ABTS•(+) scavenging, and Cu(2+)-reducing power assays, were 47.41±4.50 µM, 34.05±0.87 µM, 9.69±0.53 µM, 2.43±0.14 µM, and 1.49±0.16 µM, respectively.
  
  
  CONCLUSIONS
  Fisetin can effectively protect DNA against •OH-induced oxidative damage possibly via reactive oxygen species (ROS) scavenging approach, which is assumed to be hydrogen atom (H•) and/or single electron (e) donation (HAT/SET) pathways. In the HAT pathway, the 3',4'-dihydroxyl moiety in B ring of fisetin is thought to play an important role, because it can be ultimately oxidized to a stable ortho-benzoquinone form.
authors.affiliation: School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Waihuang East Road No.232, Guangzhou Higher Education Mega Center, 510006, Guangzhou, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Waihuang East Road No.232, Guangzhou Higher Education Mega Center, 510006, Guangzhou, China.
authors.forename: Huajuan
authors.initials: H
authors.lastname: Lin
authors.affiliation: School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Waihuang East Road No.232, Guangzhou Higher Education Mega Center, 510006, Guangzhou, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Tu
authors.affiliation: School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Waihuang East Road No.232, Guangzhou Higher Education Mega Center, 510006, Guangzhou, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Waihuang East Road No.232, Guangzhou Higher Education Mega Center, 510006, Guangzhou, China.
authors.forename: Xican
authors.initials: X
authors.lastname: Li
keywords: 3’,4’-dihydroxyl; Antioxidant mechanism; Hydrogen atom transfer; Single electron transfer mechanism; •OH-induced DNA damage