title: Lactobionic acid and carboxymethyl chitosan functionalized graphene oxide nanocomposites as targeted anticancer drug delivery systems.
abstract: In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA). Analogous systems without LA were prepared as controls. Doxorubicin (DOX) was loaded onto the composites through adsorption. The release behavior from both the LA-functionalized and the LA-free material is markedly pH sensitive. The modified GOs have high biocompatibility with the liver cancer cell line SMMC-7721, but can induce cell death after 24h incubation if loaded with DOX. Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also. These findings show that the modified GO materials are strong potential candidates for targeted anticancer drug delivery systems.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: 18201763956@163.com.
authors.forename: Qixia
authors.initials: Q
authors.lastname: Pan
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: LvYao2014year@126.com.
authors.forename: Yao
authors.initials: Y
authors.lastname: Lv
authors.affiliation: UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK. Electronic address: g.williams@ucl.ac.uk.
authors.forename: Gareth R
authors.initials: GR
authors.lastname: Williams
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: taolei89757@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Tao
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: yhhdr_2009@163.com.
authors.forename: Huihui
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: heyu.li@qq.com.
authors.forename: Heyu
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: lzhu@dhu.edu.cn.
authors.forename: Limin
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D045744:Cell Line, Tumor; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D004187:Disaccharides / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D065546:Drug Liberation; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004187:Disaccharides; D004337:Drug Carriers; D010087:Oxides; C514968:carboxymethyl-chitosan; C005608:lactobionic acid; D006108:Graphite; D048271:Chitosan
keywords: ASGPRS receptor; Doxorubicin; Graphene oxide; Lactobionic acid; Targeted delivery; pH sensitive