title: Up-regulated microRNA-155 expression is associated with poor prognosis in cervical cancer patients.
abstract: BACKGROUND
  MicroRNAs (miRNAs) play important roles in tumor development and progression. The purposes of the study was to investigate the role of miR-155 in cervical cancer.
  
  
  METHODS
  Quantitative real-time RT-PCR (qRT-PCR) was performed to examine miR-155 expression in cervical cancer tissues and adjacent non-cancerous tissues. The association with overall survival of patients was analyzed by Kaplan-Meier survival analysis. Small interfering RNA (siRNA) was used to suppress miR-155 expression in cervical cancer cells. In vitro assays were performed to further explore the biological functions of miR-155 in cervical cancer.
  
  
  RESULTS
  We found that miR-155 expression was markedly up-regulated in cervical cancer tissues and correlated with FIGO stage, lymph nodes metastasis, vascular invasion and HPV. Patients with high miR-155 expression level had poorer overall survival than those with low miR-155 expression. Furthermore, multivariate Cox regression analysis suggested that increased miR-155 was an independent prognostic indicator for cervical cancer (P=0.007; HR=2.320; 95%CI: 1.259-4.276). Moreover, knockdown of miR-155 was demonstrated to inhibit cell proliferation, migration, and invasion in vitro.
  
  
  CONCLUSIONS
  Our study presents that miR-155 is a novel molecule involved in cervical cancer progression, which provide a potential prognostic biomarker and therapeutic target.
authors.affiliation: Department of Cancer Biotherapy Center, Hebei Cancer Hospital, Shijiazhuang 050000, Hebei, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Fang
authors.affiliation: Department of Cancer Biotherapy Center, Hebei Cancer Hospital, Shijiazhuang 050000, Hebei, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Shuang
authors.affiliation: Department of Cancer Biotherapy Center, Hebei Cancer Hospital, Shijiazhuang 050000, Hebei, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Yi
authors.affiliation: Department of Cancer Biotherapy Center, Hebei Cancer Hospital, Shijiazhuang 050000, Hebei, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Sheng
authors.affiliation: Department of Cancer Biotherapy Center, Hebei Cancer Hospital, Shijiazhuang 050000, Hebei, China. Electronic address: liuysbdhyt@126.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D053208:Kaplan-Meier Estimate; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D011379:Prognosis; D016016:Proportional Hazards Models; D015854:Up-Regulation / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs
keywords: Cervical cancer; MiR-155; Overall survival; Prognosis