title: Tomato yellow leaf curl virus: No evidence for replication in the insect vector Bemisia tabaci.
abstract: Begomovirus ssDNA plant virus (family Geminiviridae) replication within the Bemisia tabaci vector is controversial. Transovarial transmission, alteration to whitefly biology, or detection of viral transcripts in the vector are proposed as indirect evidence of replication of tomato yellow leaf curl virus (TYLCV). Recently, contrasting direct evidence has been reported regarding the capacity of TYLCV to replicate within individuals of B. tabaci based on quantitave PCR approaches. Time-course experiments to quantify complementary and virion sense viral nucleic acid accumulation within B. tabaci using a recently implemented two step qPCR procedure revealed that viral DNA quantities did not increase for time points up to 96 hours after acquisition of the virus. Our findings do not support a recent report claiming TYLCV replication in individuals of B. tabaci.
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Universidad de Málaga - Consejo Superior de Investigaciones Científicas, Estación Experimental "La Mayora", 29750 Algarrobo-Costa, Málaga, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Sánchez-Campos
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Universidad de Málaga - Consejo Superior de Investigaciones Científicas, Área de Genética, Campus de Teatinos, 29071 Málaga, Spain.
authors.forename: Edgar A
authors.initials: EA
authors.lastname: Rodríguez-Negrete
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Universidad de Málaga - Consejo Superior de Investigaciones Científicas, Área de Genética, Campus de Teatinos, 29071 Málaga, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Cruzado
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Universidad de Málaga - Consejo Superior de Investigaciones Científicas, Área de Genética, Campus de Teatinos, 29071 Málaga, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Grande-Pérez
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Universidad de Málaga - Consejo Superior de Investigaciones Científicas, Área de Genética, Campus de Teatinos, 29071 Málaga, Spain.
authors.forename: Eduardo R
authors.initials: ER
authors.lastname: Bejarano
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Universidad de Málaga - Consejo Superior de Investigaciones Científicas, Estación Experimental "La Mayora", 29750 Algarrobo-Costa, Málaga, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Navas-Castillo
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Universidad de Málaga - Consejo Superior de Investigaciones Científicas, Estación Experimental "La Mayora", 29750 Algarrobo-Costa, Málaga, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Moriones
mesh_terms: D000818:Animals; D053839:Begomovirus / Q000502:physiology*; D004279:DNA, Viral; D006430:Hemiptera / Q000235:genetics / Q000821:virology*; D007303:Insect Vectors / Q000821:virology*; D018551:Solanum lycopersicum / Q000821:virology; D010935:Plant Diseases / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction; D014771:Virion / Q000502:physiology*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral