title: Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source.
abstract: Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 μm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 Ångström were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.
authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
authors.forename: Anna
authors.identifier: 0000-0002-5510-2245
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.|Institute of Physics ASCR, v.v.i. (FZU), ELI-Beamlines Project, Prague 182 21, Czech Republic.
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authors.affiliation: SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
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authors.affiliation: Center for Free Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg 22607, Germany.
authors.forename: Salah
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authors.affiliation: Center for Free Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg 22607, Germany.
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authors.affiliation: Center for Free Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg 22607, Germany.
authors.forename: Anton
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authors.affiliation: European XFEL GmbH, Holzkoppel 4, Schenefeld 22869, Germany.
authors.forename: Richard J
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authors.affiliation: Australian Research Council Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia.
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authors.lastname: Berntsen
authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.affiliation: SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
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authors.affiliation: SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.|Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439, USA.|Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstraße 36, Berlin 10623, Germany.
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authors.identifier: 0000-0001-7896-473X
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authors.affiliation: Center for Free Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg 22607, Germany.|Department of Physics, University of Hamburg, Hamburg 22761, Germany.
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.identifier: 0000-0002-1887-7551
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authors.affiliation: SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.|Stanford PULSE Institute, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
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authors.affiliation: Arizona State University, School of Molecular Sciences (SMS), Tempe, Arizona 85287-1604, USA.|Biodesign Center for Applied Structural Discovery, Biodesign Institute at Arizona State University, Tempe 85287, USA.
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.affiliation: Biodesign Center for Applied Structural Discovery, Biodesign Institute at Arizona State University, Tempe 85287, USA.|Arizona State University, School of Life Sciences (SOLS), Tempe, Arizona 85287-5401, USA.|Biodesign Center for Infectious Diseases and Vaccinology, Biodesign Institute at Arizona State University, Tempe 85287, USA.
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authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
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authors.affiliation: Biodesign Center for Applied Structural Discovery, Biodesign Institute at Arizona State University, Tempe 85287, USA.|Arizona State University, Department of Physics, Tempe, Arizona 85287, USA.
authors.forename: Richard A
authors.initials: RA
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.initials: HK
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authors.affiliation: Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, New York 14853, USA.
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.initials: DS
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.identifier: 0000-0002-2141-438X
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authors.affiliation: European XFEL GmbH, Holzkoppel 4, Schenefeld 22869, Germany.
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.affiliation: Department of Physics, Pohang University of Science and Technology, Pohang 37673, Korea.
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authors.affiliation: Arizona State University, Department of Physics, Tempe, Arizona 85287, USA.
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.|Department of Information Technology, Science for Life Laboratory, Uppsala University, Lägerhyddsvägen 2 (Box 337), Uppsala SE-75105, Sweden.
authors.forename: Carl
authors.initials: C
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.identifier: 0000-0001-9946-3889
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authors.affiliation: Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, Hamburg D-22607, Germany.
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.affiliation: Department of Physics, University of Wisconsin Milwaukee, 3135 North Maryland Ave., Milwaukee, Wisconsin 53211, USA.
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.|Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, Stockholm SE-106 91, Sweden.
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authors.identifier: 0000-0003-2793-5052
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authors.affiliation: SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.|Stanford PULSE Institute, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
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authors.affiliation: Department of Physics, Pohang University of Science and Technology, Pohang 37673, Korea.
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.|Department of Physics and Astronomy, Uppsala University, Lägerhyddsvägen 1 (Box 516), Uppsala SE-75120, Sweden.
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authors.affiliation: Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, Hamburg D-22607, Germany.|National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Kashirskoe shosse 31, Moscow 115409, Russia.
authors.forename: Ivan A
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authors.affiliation: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
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authors.affiliation: Center for Free Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg 22607, Germany.|Department of Physics, University of Hamburg, Hamburg 22761, Germany.
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authors.affiliation: Brookhaven National Laboratory, NSLS-II, Upton, New York 11973, USA.
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authors.affiliation: Center for Free Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg 22607, Germany.|Department of Physics, University of Hamburg, Hamburg 22761, Germany.|Max-Planck Institute for the Structure and Dynamics of Matter, CFEL, Hamburg 22607, Germany.
authors.forename: Paulraj Lourdu
authors.initials: PL
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authors.affiliation: SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
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authors.initials: CH
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authors.affiliation: Arizona State University, School of Molecular Sciences (SMS), Tempe, Arizona 85287-1604, USA.|Biodesign Center for Applied Structural Discovery, Biodesign Institute at Arizona State University, Tempe 85287, USA.
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mesh_terms: D000465:Algorithms; D012275:Oryza / Q000821:virology*; D010315:Particle Accelerators; D012087:Reoviridae / Q000302:isolation & purification*; D014771:Virion; D014965:X-Rays