title: Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish.
abstract: The zebrafish has emerged as an important model organism for understanding the cellular and molecular mechanisms of tissue regeneration. Adult zebrafish efficiently replace cardiac muscle after partial resection of their ventricle, or after transgenic ablation of cardiomyocytes. Here, we describe methodology for inducing these injuries and assaying indicators of regeneration.
authors.affiliation: Department of Cell Biology, Duke University Medical Center, 466 Nanaline Duke Building, Box 3709, Durham, NC, 27710, USA.
authors.forename: Jinhu
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cell Biology, Duke University Medical Center, 466 Nanaline Duke Building, Box 3709, Durham, NC, 27710, USA. kenneth.poss@duke.edu.
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Poss
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D006321:Heart / Q000502:physiology* / Q000503:physiopathology*; D006335:Heart Injuries / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D006352:Heart Ventricles / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology; D012038:Regeneration / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D014945:Wound Healing / Q000502:physiology; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029961:Zebrafish Proteins
keywords: Ablation; Cardiomyocyte; Heart; Regeneration; Surgery; Zebrafish