title: RNAi pathway participates in chromosome segregation in mammalian cells.
abstract: The RNAi machinery is a mighty regulator in a myriad of life events. Despite lines of evidence that small RNAs and components of the RNAi pathway may be associated with structure and behavior of mitotic chromosomes in diverse organisms, a direct role of the RNAi pathway in mammalian mitotic chromosome segregation remains elusive. Here we report that Dicer and AGO2, two central components of the mammalian RNAi pathway, participate in the chromosome segregation. Knockdown of Dicer or AGO2 results in a higher incidence of chromosome lagging, and this effect is independent from microRNAs as examined with DGCR8 knockout cells. Further investigation has revealed that α-satellite RNA, a noncoding RNA derived from centromeric repeat region, is managed by AGO2 under the guidance of endogenous small interference RNAs (ASAT siRNAs) generated by Dicer. Furthermore, the slicer activity of AGO2 is essential for the chromosome segregation. Level and distribution of chromosome-associated α-satellite RNA have crucial regulatory effect on the localization of centromeric proteins such as centromere protein C1 (CENPC1). With these results, we also provide a paradigm in which the RNAi pathway participates in vital cellular events through the maintenance of level and distribution of noncoding RNAs in cells.
authors.affiliation: School of Life Sciences, CAS Key Laboratory of Brain Function and Disease, University of Science and Technology of China , Hefei, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Huang
authors.affiliation: School of Life Sciences, CAS Key Laboratory of Brain Function and Disease, University of Science and Technology of China , Hefei, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Sciences, CAS Key Laboratory of Brain Function and Disease, University of Science and Technology of China , Hefei, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Life Sciences, CAS Key Laboratory of Brain Function and Disease, University of Science and Technology of China , Hefei, China.
authors.forename: Shuhuan
authors.initials: S
authors.lastname: Cao
authors.affiliation: School of Life Sciences, CAS Key Laboratory of Brain Function and Disease, University of Science and Technology of China , Hefei, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Shan
keywords: AGO2; ASAT siRNA; CENPC1; Dicer; RNAi pathway; chromosome segregation; α-satellite RNA