title: Melatonin modulates the functions of porcine granulosa cells via its membrane receptor MT2 in vitro.
abstract: Melatonin (N-acetyl-5-methoxytryptamine) is documented as a hormone involved in the circadian regulation of physiological and neuroendocrine function in mammals. Herein, the effects of melatonin on the functions of porcine granulosa cells in vitro were investigated. Porcine granulosa cells were cultivated with variable concentrations of melatonin (0, 0.001, 0.01, 0.1, 1.0, and 10ng/mL) for 48h. Melatonin receptor agonist (IIK7) and antagonist (Luzindole, 4P-PDOT) were used to further examine the action of melatonin. The results showed optimum cell viability and colony-forming efficiency of porcine granulosa cells at 0.01ng/mL melatonin for 48-h incubation period. The percentage of apoptotic granulosa cells was significantly reduced by 0.01 and 0.1ng/mL melatonin within the 48-h incubation period as compared with the rest of the treatments. Estradiol biosynthesis was significantly stimulated by melatonin supplementation and suppressed for the progesterone secretion; the minimum ratio of progesterone to estradiol was 1.82 in 0.01ng/mL melatonin treatment after 48h of cultivation. Moreover, the expression of BCL-2, CYP17A1, CYP19A1, SOD1, and GPX4 were up-regulated by 0.01ng/mL melatonin or combined with IIK7, but decreased for the mRNA levels of BAX, P53, and CASPASE-3, as compared with control or groups treated with Luzindole or 4P-PDOT in the presence of melatonin. In conclusion, the study demonstrated that melatonin mediated proliferation, apoptosis, and steroidogenesis in porcine granulosa cells predominantly through the activation of melatonin receptor MT2 in vitro, which provided evidence of the beneficial role of melatonin as well as its functional mechanism in porcine granulosa cells in vitro.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Ya-Mei
authors.initials: YM
authors.lastname: He
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Deng
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Mei-Hong
authors.initials: MH
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Bello Musa
authors.initials: BM
authors.lastname: Bodinga
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Hua-Li
authors.initials: HL
authors.lastname: Chen
authors.affiliation: College of Environment and Chemistry Engineering, Yanshan University, Qinhuangdao 066004, Hebei, PR China.
authors.forename: Zeng-Sheng
authors.initials: ZS
authors.lastname: Han
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Zhong-Liang
authors.initials: ZL
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, PR China; College of Environment and Chemistry Engineering, Yanshan University, Qinhuangdao 066004, Hebei, PR China. Electronic address: liqw@nwsuaf.edu.cn.
authors.forename: Qing-Wang
authors.initials: QW
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000502:physiology*; D054833:Isoindoles / Q000494:pharmacology; D008550:Melatonin / Q000494:pharmacology*; D044123:Receptor, Melatonin, MT2 / Q000235:genetics / Q000378:metabolism*; D013552:Swine / Q000502:physiology*; D014363:Tryptamines / Q000494:pharmacology
chemical_list: C121417:IIK7; D054833:Isoindoles; D044123:Receptor, Melatonin, MT2; D014363:Tryptamines; C057154:luzindole; D008550:Melatonin
keywords: Apoptosis; Granulosa cells; Melatonin; Porcine; Steroidogenesis