title: The use of serum anti-Mullerian hormone (AMH) levels and antral follicle count (AFC) to predict the number of oocytes collected and availability of embryos for cryopreservation in IVF.
abstract: OBJECTIVE
  To investigate the predictive value of anti-Mullerian hormone (AMH) and antral follicle count (AFC) on the final number of oocytes retrieved and the availability of embryos for cryopreservation in in vitro fertilization (IVF) cycles.
  
  
  METHODS
  In this prospective study, one hundred and twenty women in their first IVF treatment were enrolled. The short stimulation agonist protocol was used for controlled ovarian hyperstimulation in all cases. Serum AMH levels were measured during the menstrual cycle preceding treatment. AFC was measured in cycle day 2, just before starting ovarian stimulation.
  
  
  RESULTS
  A strong, positive correlation between AMH, AFC and the number of collected oocytes was found. The patients with available and suitable supplementary embryos for cryopreservation had higher levels of AMH and larger numbers of AFC.
  
  
  CONCLUSIONS
  AMH and AFC appear to be valuable markers mainly for ovarian reserve and response to IVF treatment. Serum AMH levels and AFC are significantly associated with the number of retrieved oocytes. Also, a positive correlation with the availability of supernumerary embryos suitable for cryopreservation was observed.
authors.affiliation: Laboratory of Reproductive Physiology - In Vitro Fertilization, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece. drkotanidis@gmail.com.|, Florou 22A Kordelio, 56334, Thessaloníki, Greece. drkotanidis@gmail.com.
authors.forename: L
authors.initials: L
authors.lastname: Kotanidis
authors.affiliation: Embryokosmogenesis IVF Unit, Alexandroupolis, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Nikolettos
authors.affiliation: 3rd Department of Obstetrics and Gynaecology, Aristotle University of Thessaloniki, Thessaloníki, Greece.
authors.forename: S
authors.initials: S
authors.lastname: Petousis
authors.affiliation: Laboratory of Physiology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
authors.forename: B
authors.initials: B
authors.lastname: Asimakopoulos
authors.affiliation: 4th DYPE, Thessaloníki, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Chatzimitrou
authors.affiliation: Laboratory of Pharmacology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Kolios
authors.affiliation: Laboratory of Reproductive Physiology - In Vitro Fertilization, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.|Embryokosmogenesis IVF Unit, Alexandroupolis, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Nikolettos
mesh_terms: D000328:Adult; D054304:Anti-Mullerian Hormone / Q000097:blood*; D015415:Biomarkers / Q000097:blood*; D015925:Cryopreservation / Q000379:methods*; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology* / Q000000981:diagnostic imaging / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D009865:Oocytes / Q000166:cytology*; D006080:Ovarian Follicle / Q000166:cytology* / Q000000981:diagnostic imaging / Q000378:metabolism; D011446:Prospective Studies; D014463:Ultrasonography; D055815:Young Adult
chemical_list: D015415:Biomarkers; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone
keywords: AFC; AMH; Cryopreservation; Embryos; IVF; Oocytes