title: [The Protective Effect of Target Temperature Management Combined with Prostaglandin E₁ on Ischemia/Reperfusion Injury of Cerebral Micro-vascular Endothelium of ROSC Rat].
abstract: OBJECTIVE
  To investigate the protective effect of target temperature management (TTM) combined with prostaglandin E₁ (PGE₁) on ischemia/reperfusion (I/R) injury of cerebral micro-vascular endothelium cell (CMEC) in the return of spontaneous circulation (ROSC) rats with successful cardiopulmonary resuscitation.
  
  
  METHODS
  Transoesophageal cardiac pacing with alternating current was used to induce ventricular fibrillation in rats. Five groups were set: Sham group (S group), ROSC group (R group), PGE₁ group (P group), TTM group (T group) and PGE₁/TTM group (PT group). Cell edema and micro-thrombus formation in cerebral tissue were evaluated through HE staining. I/R injury of CMEC was evaluated through CD34/TUNEL and vascular endothelial (VE)-cadherin/VE growth factor receptor double fluorescent immunohistochemistry staining. VE-cadherin mRNA and vascular cell adhesion molecule-1 (VCAM-1) mRNA expression in cerebral tissue lysate was analyzed by fluorescence quantitative PCR.
  
  
  RESULTS
  TTM, PGE₁ and PGE₁/TTM could significantly improve cerebral interstitial edema, micro-thrombus, and inflammatory cells infiltration in the brain tissue, and reduce the apoptosis and VE-cadherin protein loss of CMEC. PGE₁/TTM showed better protective effect. These 3 interventions also inhibited the rapid elevation of VE-cadherin mRNA (0. 5 h) and VCAM-1 mRNA (4 h and 8 h) expression (P < 0.05), which might indicate the less I/R injury of CMEC of ROSC rat.
  
  
  CONCLUSIONS
  Both PGE₁ and TTM could alleviate I/R injury of CMEC from ROSC rat after CPR separately, while PGE₁/TTM combined intervention might have synergistic better effect.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Shi-chao
authors.initials: SC
authors.lastname: Lai
authors.forename: Yong
authors.initials: Y
authors.lastname: Xie
authors.forename: Bo-fu
authors.initials: BF
authors.lastname: Liu
authors.forename: Ya-rong
authors.initials: YR
authors.lastname: He
authors.forename: Hai
authors.initials: H
authors.lastname: Hu
authors.forename: Zhia
authors.initials: Z
authors.lastname: Wan
mesh_terms: D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy*; D013696:Temperature; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism; D000099152:Cadherin 5
chemical_list: D000527:Alprostadil; D015703:Antigens, CD; D015820:Cadherins; D019010:Vascular Cell Adhesion Molecule-1; D000099152:Cadherin 5