title: Evaluation of the radioprotective effect of Turkish propolis on foreskin fibroblast cells.
abstract: OBJECTIVE
  Propolis is a resinous bee product, rich of polyphenolic compounds and flavonoids. It is known that in different geographic zones its chemical composition varies due to the different plant sources. Many biological properties including antimicrobial, antioxidative, anti-inflammatory, antitumoral, antigenotoxic, antimutagenic, cytostatic activities have been ascribed to propolis. These biological effects are predominantly attributed to its content of polyphenols. In this study, we aimed to evaluate the radioprotective effect of ethanolic extract of Turkish propolis. (EETP) against γ-ray-induced DNA damage on fibroblast cells using comet assay for the first time.
  
  
  METHODS
  Fibroblast cells were pretreated 15 and 30 min with concentrations of 100, 200 and 300 μg/mL EETP then they were exposed to 3 Gy γ-rays. Amifostine (synthetic aminothiol compound) was used as a positive control.
  
  
  RESULTS
  The results showed a significant decrease in γ-ray-induced DNA damage on cells treated with EETP and amifostine when compared to only irradiated cells. (P < 001).
  
  
  CONCLUSIONS
  It was concluded that EETP prevent γ-ray-induced DNA damage in fibroblast cells and might have radioprotective activity.
authors.affiliation: Department of Pharmaceutical Toxicology, Institute of Health Sciences, Ankara University, 06100 Ankara, Turkey.
authors.forename: Can Ozgur
authors.initials: CO
authors.lastname: Yalcin
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey.
authors.forename: Yuksel
authors.initials: Y
authors.lastname: Aliyazicioglu
authors.affiliation: Department of Medical Biochemistry, Institute of Health Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey.
authors.forename: Selim
authors.initials: S
authors.lastname: Demir
authors.affiliation: Department of Genetic and Bioengineering, Faculty of Engineering and Natural Sciences, Gumushane University; Traditional Medicine Practice and Research Center, Gumushane University, 29100 Gumushane, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Turan
authors.affiliation: Department of Radiation Oncology, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey.
authors.forename: Zumrut
authors.initials: Z
authors.lastname: Bahat
authors.affiliation: Department of Medical Biochemistry, Institute of Health Sciences, Karadeniz Technical University, 61080 Trabzon; Department of Biochemistry, Faculty of Pharmacy, Cumhuriyet University, 58140 Sivas, Turkey.
authors.forename: Sema
authors.initials: S
authors.lastname: Misir
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey.
authors.forename: Orhan
authors.initials: O
authors.lastname: Deger
mesh_terms: D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects; D011429:Propolis / Q000737:chemistry / Q000494:pharmacology*; D011827:Radiation; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors; D014421:Turkey
chemical_list: D011837:Radiation-Protective Agents; D011429:Propolis