title: Single nucleotide polymorphisms in an intergenic chromosome 2q region associated with tissue factor pathway inhibitor plasma levels and venous thromboembolism.
abstract: Essentials Tissue factor pathway inhibitor (TFPI) regulates the blood coagulation cascade. We replicated previously reported linkage of TFPI plasma levels to the chromosome 2q region. The putative causal locus, rs62187992, was associated with TFPI plasma levels and thrombosis. rs62187992 was marginally associated with TFPI expression in human aortic endothelial cells. Click to hear Ann Gil's presentation on new insights into thrombin activatable fibrinolysis inhibitor SUMMARY: Background Tissue factor pathway inhibitor (TFPI) regulates fibrin clot formation, and low TFPI plasma levels increase the risk of arterial thromboembolism and venous thromboembolism (VTE). TFPI plasma levels are also heritable, and a previous linkage scan implicated the chromosome 2q region, but no specific genes. Objectives To replicate the finding of the linkage region in an independent sample, and to identify the causal locus. Methods We first performed a linkage analysis of microsatellite markers and TFPI plasma levels in 251 individuals from the F5L Family Study, and replicated the finding of the linkage peak on chromosome 2q (LOD = 3.06). We next defined a follow-up region that included 112 603 single nucleotide polymorphisms (SNPs) under the linkage peak, and meta-analyzed associations between these SNPs and TFPI plasma levels across the F5L Family Study and the Marseille Thrombosis Association (MARTHA) Study, a study of 1033 unrelated VTE patients. SNPs with false discovery rate q-values of < 0.10 were tested for association with TFPI plasma levels in 892 patients with coronary artery disease in the AtheroGene Study. Results and Conclusions One SNP, rs62187992, was associated with TFPI plasma levels in all three samples (β = + 0.14 and P = 4.23 × 10-6combined; β = + 0.16 and P = 0.02 in the F5L Family Study; β = + 0.13 and P = 6.3 × 10-4in the MARTHA Study; β = + 0.17 and P = 0.03 in the AtheroGene Study), and contributed to the linkage peak in the F5L Family Study. rs62187992 was also associated with clinical VTE (odds ratio 0.90, P = 0.03) in the INVENT Consortium of > 7000 cases and their controls, and was marginally associated with TFPI expression (β = + 0.19, P = 0.08) in human aortic endothelial cells, a primary site of TFPI synthesis. The biological mechanisms underlying these associations remain to be elucidated.
authors.affiliation: Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Dennis
authors.affiliation: Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario, Canada.
authors.forename: V
authors.initials: V
authors.lastname: Truong
authors.affiliation: Sorbonne Universités, UPMC Univ. Paris 06, Paris, France.|INSERM, UMR_S 1166, Paris, France.|ICAN Institute for Cardiometabolism and Nutrition, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Aïssi
authors.affiliation: Laboratorio Internacional de Investigación sobre el Genoma Humano, Universidad Nacional Autónoma de México, Santiago de Querétaro, Mexico.|Genetics and Genome Biology, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Medina-Rivera
authors.affiliation: Department of General and Interventional Cardiology, University of Hamburg, Hamburg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Blankenberg
authors.affiliation: Sorbonne Universités, UPMC Univ. Paris 06, Paris, France.|INSERM, UMR_S 1166, Paris, France.|ICAN Institute for Cardiometabolism and Nutrition, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Germain
authors.affiliation: Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Lemire
authors.affiliation: Genetics and Genome Biology, Hospital for Sick Children, Toronto, Ontario, Canada.|Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Antounians
authors.affiliation: Center for Public Health Genomics, Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Civelek
authors.affiliation: Department of General and Interventional Cardiology, University of Hamburg, Hamburg, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Schnabel
authors.affiliation: Ottawa Hospital Research Institute, Ottawa, Ontario, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Wells
authors.affiliation: Genetics and Genome Biology, Hospital for Sick Children, Toronto, Ontario, Canada.|Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
authors.forename: M D
authors.initials: MD
authors.lastname: Wilson
authors.affiliation: INSERM, UMR_S 1062, Marseille, France.|Inra, UMR_INRA 1260, Marseille, France.|Aix Marseille Université, Marseille, France.
authors.forename: P-E
authors.initials: PE
authors.lastname: Morange
authors.affiliation: Sorbonne Universités, UPMC Univ. Paris 06, Paris, France.|INSERM, UMR_S 1166, Paris, France.|ICAN Institute for Cardiometabolism and Nutrition, Paris, France.
authors.forename: D-A
authors.initials: DA
authors.lastname: Trégouët
authors.affiliation: Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario, Canada. france.gagnon@utoronto.ca.
authors.forename: F
authors.initials: F
authors.lastname: Gagnon
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001011:Aorta / Q000473:pathology; D001777:Blood Coagulation; D002648:Child; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D003324:Coronary Artery Disease / Q000097:blood; D042783:Endothelial Cells / Q000166:cytology; D005165:Factor V / Q000235:genetics; D005189:False Positive Reactions; D005260:Female; D008040:Genetic Linkage; D006720:Homozygote; D006801:Humans; D008074:Lipoproteins / Q000097:blood* / Q000235:genetics; D008126:Lod Score; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D013927:Thrombosis / Q000097:blood; D054556:Venous Thromboembolism / Q000097:blood* / Q000235:genetics
chemical_list: D008074:Lipoproteins; C095381:factor V Leiden; C051928:lipoprotein-associated coagulation inhibitor; D005165:Factor V
keywords: blood coagulation; genetic association studies; genetic linkage; thrombosis; tissue factor pathway inhibitor