title: A Living Eukaryotic Autocementation Kit from Surface Display of Silica Binding Peptides on Yarrowia lipolytica.
abstract: With the development of civil engineering, the demand for suitable cementation materials is increasing rapidly. However, traditional cementation methods are not eco-friendly enough and more sustainable approach such as biobased cementation is required. To meet such demand, Euk.cement, a living eukaryotic cell-based biological autocementation kit, was created in this work. Through the surface display of different silica binding peptides on the fungus Yarrowia lipolytica, Euk.cement cells can immobilize onto any particles with a silica containing surface with variable binding intensity. Meanwhile, recombinant MCFP3 released from the cells will slowly consolidate this binding of cells to particles. The metabolism of immobilized living cells will finally complete the carbonate sedimentation and tightly stick the particles together. The system is designed to be initiated by blue light, making it controllable. This autocementation kit can be utilized for industrial and environmental applications that fit our concerns on making the cementation process eco-friendly.
authors.forename: Shuyan
authors.initials: S
authors.lastname: Tang
authors.forename: Wang
authors.initials: W
authors.lastname: Xi
authors.forename: Zhangyu
authors.initials: Z
authors.lastname: Cheng
authors.forename: Lei
authors.initials: L
authors.lastname: Yin
authors.forename: Ruihao
authors.initials: R
authors.lastname: Li
authors.forename: Guozhao
authors.initials: G
authors.lastname: Wu
authors.forename: Wangjie
authors.initials: W
authors.lastname: Liu
authors.forename: Junjie
authors.initials: J
authors.lastname: Xu
authors.affiliation: Bei Shizhang Advanced Class of Life Science Research, co-founded by Huazhong University of Science and Technology, 430074, Wuhan, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, and University of Chinese Academy of Sciences , 100049, Beijing, P. R. China.
authors.forename: Shuaiying
authors.initials: S
authors.lastname: Xiang
authors.forename: Yanxiao
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Bei Shizhang Advanced Class of Life Science Research, co-founded by Huazhong University of Science and Technology, 430074, Wuhan, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, and University of Chinese Academy of Sciences , 100049, Beijing, P. R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ge
authors.forename: Kang
authors.initials: K
authors.lastname: Ning
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Bei Shizhang Advanced Class of Life Science Research, co-founded by Huazhong University of Science and Technology, 430074, Wuhan, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, and University of Chinese Academy of Sciences , 100049, Beijing, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhan
mesh_terms: D018914:Cells, Immobilized; D056890:Eukaryota / Q000145:classification*; D010455:Peptides / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D025062:Yarrowia
chemical_list: D010455:Peptides; D011994:Recombinant Proteins; D012822:Silicon Dioxide
keywords: Yarrowia lipolytica; autocementation; silica binding peptides; surface display