title: Deinococcus seoulensis sp. nov., a bacterium isolated from sediment at Han River in Seoul, Republic of Korea.
abstract: Strain 16F1E(T) was isolated from a 3-kGy-irradiated sediment sample collected at Han River in Seoul, Republic of Korea. Cells of this strain were observed to be Gram-positive, pililike structure, and short rod shape, and colonies were red in color. The strain showed the highest degree of 16S rRNA gene sequence similarity to Deinococcus aquaticus PB314(T) (98.8%), Deinococcus depolymerans TDMA-24(T) (98.1%), Deinococcus caeni Ho-08(T) (98.0%), and Deinococcus grandis DSM 3963(T) (97.0%). 16S rRNA gene sequence analysis identified this strain as a member of the genus Deinococcus (Family: Deinococcaceae). The genomic DNA G+C content of strain 16F1ET was 66.9 mol%. The low levels of DNA-DNA hybridization (< 56.2%) with the species mentioned above identified strain 16F1E(T) as a novel Deinococcus species. Its oxidase and catalase activities as well as the production of acid from glucose were positive. Growth of the strain was observed at 10-37°C (optimum: 20-30°C) and pH 4-10 (optimum: pH 7-8). The cells tolerated less than 5% NaCl and had low resistance to gamma radiation (D10 < 4 kGy). Strain 16F1ET possessed the following chemotaxonomic characteristics: C16:0, C15:1 ω6c, and C16:1 ω7c as the major fatty acids; phosphoglycolipid as the predominant polar lipid; and menaquinone-8 as the predominant respiratory isoprenoid quinone. Based on the polyphasic evidence, as well as the phylogenetic, genotypic, phenotypic, and chemotaxonomic characterization results, strain 16F1E(T) (=KCTC 33793(T) =JCM 31404(T)) is proposed to represent the type strain of a novel species, Deinococcus seoulensis sp. nov.
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea.
authors.forename: Sangyong
authors.initials: S
authors.lastname: Lim
authors.affiliation: Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea.
authors.forename: Sun-Wook
authors.initials: SW
authors.lastname: Jeong
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Seung-Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: School of Food Science and Biotechnology/Food and Bio-Industry Research Institute, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Young-Je
authors.initials: YJ
authors.lastname: Cho
authors.affiliation: Department of Bio and Environmental Technology, Seoul Women's University, Seoul, 01797, Republic of Korea.
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea. heeyoung@knu.ac.kr.|Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea. heeyoung@knu.ac.kr.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D034301:Deinococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D045483:Rivers / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: Deinococcus; gamma-radiation resistance; polyphasic taxonomy