title: Prevalence of naturally occurring antibodies against dog erythrocyte antigen 7 in a population of dog erythrocyte antigen 7-negative dogs from Spain and Italy.
abstract: OBJECTIVE To determine the prevalence of naturally occurring anti-dog erythrocyte antigen (DEA) 7 antibodies in DEA 7-negative dogs from Spain and Italy. ANIMALS 252 DEA 7-negative dogs from a population of 312 dogs that were previously tested for DEA 1, DEA 4, and DEA 7. PROCEDURES A plasma sample was obtained from each dog and evaluated for anti-DEA 7 antibodies by the use of gel column agglutination. Each plasma sample underwent major crossmatching with RBCs from DEA 7-positive dogs. Samples that resulted in agglutination were then crossmatched with RBCs from DEA 1-negative, DEA 4-positive, and DEA 7-negative dogs to confirm the presence of anti-DEA 7 antibodies. Results were then used to calculate the risk for a delayed transfusion reaction in a DEA 7-negative dog with anti-DEA 7 antibodies after a transfusion with blood that was not crossmatched or typed for DEA 7. RESULTS 96 of 252 (38.1%) plasma samples contained anti-DEA 7 antibodies. A DEA 7-negative dog with anti-DEA 7 antibodies had a 5.9% chance of developing a delayed hemolytic reaction after transfusion with blood not crossmatched or typed for DEA 7. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that canine blood used for transfusion should be crossmatched with the blood or plasma of the intended recipient prior to transfusion to minimize the likelihood that the recipient will develop a hemolytic reaction associated with anti-DEA 7 antibodies. Ideal canine blood donors should be negative for both DEA 1 and DEA 7.
authors.forename: Eva
authors.initials: E
authors.lastname: Spada
authors.forename: Daniela
authors.initials: D
authors.lastname: Proverbio
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: Viñals Flórez
authors.forename: Maria
authors.initials: M
authors.lastname: Del Rosario Perlado Chamizo
authors.forename: Blanca
authors.initials: B
authors.lastname: Serra Y Gómez de la Serna
authors.forename: Roberta
authors.initials: R
authors.lastname: Perego
authors.forename: Luciana
authors.initials: L
authors.lastname: Baggiani
mesh_terms: D000818:Animals; D001782:Blood Donors; D001789:Blood Group Antigens / Q000097:blood*; D001787:Blood Group Incompatibility / Q000453:epidemiology / Q000662:veterinary*; D001788:Blood Grouping and Crossmatching / Q000662:veterinary*; D001803:Blood Transfusion / Q000662:veterinary; D004285:Dogs; D007558:Italy / Q000453:epidemiology; D010375:Pedigree; D015995:Prevalence; D013030:Spain / Q000453:epidemiology
chemical_list: D001789:Blood Group Antigens