title: Acoustofluidic harvesting of microalgae on a single chip.
abstract: We present an on-chip acoustofluidic platform for harvesting a target microalgal species from a heterogeneous population of cells and particles based on their size, density, and compressibility in a rapid, non-invasive, energy-efficient, continuously running, and automated manner. For our proof-of-principle demonstration, we use Euglena gracilis as a target species. Specifically, we show the simultaneous separation and enrichment of E. gracilis from a mixed population of E. gracilis in pond water (consisting of other microalgae and various kinds of particles as contaminants) on a single acoustofluidic chip with a recovery ratio of 92.6%, a target separation ratio of 90.1%, a concentration factor of 3.43, an enrichment factor of 12.76, and a cell viability rate of 98.3% at a high volume rate of 500 μl/min. Our results indicate that the on-chip acoustofluidic platform is an effective tool for harvesting target microalgae from mixed populations of microalgae and other contaminants.
authors.affiliation: Department of Chemistry, University of Tokyo , Tokyo 113-0033, Japan.
authors.forename: Jee-Woong
authors.initials: JW
authors.lastname: Park
authors.affiliation: Institute of Industrial Science, University of Tokyo , Tokyo 153-8505, Japan.
authors.forename: Soo Hyeon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Institute for Advanced Biosciences, Keio University , Tsuruoka 997-0035, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Ito
authors.affiliation: Institute of Industrial Science, University of Tokyo , Tokyo 153-8505, Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Fujii
authors.affiliation: Department of Biomedical Engineering, Dongguk University , Seoul, South Korea.
authors.forename: So Youn
authors.initials: SY
authors.lastname: Kim
authors.forename: Thomas
authors.initials: T
authors.lastname: Laurell
authors.affiliation: Department of Chemistry, University of Tokyo , Tokyo 113-0033, Japan.
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Lee
authors.forename: Keisuke
authors.initials: K
authors.lastname: Goda