title: miR-137 plays tumor suppressor roles in gastric cancer cell lines by targeting KLF12 and MYO1C.
abstract: Aberrant expression of miR-137 has been reported in many kinds of cancers, but its mechanisms seem to be diversely. In the present study, we compared the expression level of miR-137 in 18 paired gastric cancer (GC) samples and surgical margin (SM) samples by RNA extraction and quantitative real-time PCR (QRT-PCR). Then, we investigated the effects of miR-137 on cell proliferation, cell cycle, and cell migration separately by cell growth counting assay, cell cycle analysis, and transwell assay. Candidate targets of miR-137 were selected by biological information analysis from the intersection of miRDB, Pictar, and TarScan. Finally, mRNA and protein expression level of Krűppel-like factor 12 (KLF12) and Myosin 1C (MYO1C) were tested by QRT-PCR and western blotting assay, followed by the Luciferase reporter assay to investigate the direct interaction between them and miR-137. The results showed that miR-137 was down-regulated in GC samples than in SM samples. The expression level of miR-137 was significantly higher in patients without the vascular embolus than those with vascular embolus. And the overall survival time of patients with high miR-137 expression was longer than those with low miR-137 expression. Over expression of miR-137 could inhibit the cell migration, proliferation, and promote cell cycle arrest in G0/G1 stage in BGC-823 and SGC-7901 cell lines. KLF12 and MYO1C might be the candidate target genes of miR-137 with direct interactions between them and miR-137. In conclusion, miR-137 plays tumor suppressor roles in gastric cancer cell lines by targeting KLF12 and MYO1C.
authors.affiliation: Ningbo Institute of Medical Science, Yang-Shan-Lu, No.42-46, Jiangbei District, Ningbo, China. duyantao_2000@163.com.
authors.forename: Yantao
authors.initials: Y
authors.lastname: Du
authors.affiliation: Ningbo Institute of Medical Science, Yang-Shan-Lu, No.42-46, Jiangbei District, Ningbo, China.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Ningbo Institute of Medical Science, Yang-Shan-Lu, No.42-46, Jiangbei District, Ningbo, China.
authors.forename: Furong
authors.initials: F
authors.lastname: Wang
authors.affiliation: Peking University Cancer Hospital and Institute, Fu-Cheng-Lu, No.52, Haidian District, Beijing, China.
authors.forename: Liankun
authors.initials: L
authors.lastname: Gu
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D024461:Myosin Type I / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; C436492:KLF12 protein, human; D051741:Kruppel-Like Transcription Factors; C545567:MIRN137 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D024461:Myosin Type I; C460367:MYO1C protein, human
keywords: Gastric cancer; KLF12; MYO1C; miR-137