title: Investigation into the interaction of losartan with human serum albumin and glycated human serum albumin by spectroscopic and molecular dynamics simulation techniques: A comparison study.
abstract: The interaction between losartan and human serum albumin (HSA), as well as its glycated form (gHSA) was studied by multiple spectroscopic techniques and molecular dynamics simulation under physiological conditions. The binding information, including the binding constants, effective quenching constant and number of binding sites showed that the binding partiality of losartan to HSA was higher than to gHSA. The findings of three-dimensional fluorescence spectra demonstrated that the binding of losartan to HSA and gHSA would alter the protein conformation. The distances between Trp residue and the binding sites of the drug were evaluated on the basis of the Förster theory, and it was indicated that non-radiative energy transfer from HSA and gHSA to the losartan happened with a high possibility. According to molecular dynamics simulation, the protein secondary and tertiary structure changes were compared in HSA and gHSA for clarifying the obtained results.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, 7915893144, Iran. Electronic address: f.moeinpour@gmail.com.
authors.forename: Farid
authors.initials: F
authors.lastname: Moeinpour
authors.affiliation: Department of Chemistry, Faculty of Sciences, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, 7915893144, Iran.
authors.forename: Fatemeh S
authors.initials: FS
authors.lastname: Mohseni-Shahri
authors.affiliation: Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Bizhan
authors.initials: B
authors.lastname: Malaekeh-Nikouei
authors.affiliation: Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Hooriyeh
authors.initials: H
authors.lastname: Nassirli
mesh_terms: D001665:Binding Sites; D004735:Energy Transfer; D017127:Glycation End Products, Advanced; D006801:Humans; D019808:Losartan / Q000737:chemistry / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D011485:Protein Binding; D011487:Protein Conformation; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods; D000093423:Glycated Serum Albumin
chemical_list: D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D019808:Losartan; D000093423:Glycated Serum Albumin
keywords: Glycated human serum albumin; Human serum albumin; Losartan; Molecular dynamics simulation; Spectroscopy