title: BMP2, 4 and 6 and BMPR1B are altered from early stages of bovine cystic ovarian disease development.
abstract: Cystic ovarian disease (COD) is an important cause of subfertility in dairy cattle. Bone morphogenetic proteins (BMPs), mainly BMP2, BMP4 and BMP6, play a key role in female fertility. In this study, we hypothesized that an altered BMP system is associated with ovarian alterations contributing to COD pathogenesis. Therefore, we examined the expression of BMP2, BMP4 and BMP6 and BMP receptor 1B (BMPR1B) in the ovaries of animals with spontaneous or ACTH-induced COD, as well as during the development of the disease, in a model of follicular persistence induced by low doses of progesterone (at 5, 10 and 15 days of follicular persistence). Results showed changes in BMP2, BMP4 and BMP6 expression during folliculogenesis, in granulosa and theca cells in the COD groups, as well as at different stages of follicular persistence. Results also showed changes in BMPR1B expression in developing follicles in animals with COD, and at the initial stages of follicular persistence (P5). Comparison between groups showed significant differences, mainly in BMP4 and BMP6 expression, in granulosa and theca cells of different follicular categories. The expression of these BMPs also increased in cystic and persistent follicles, in relation to antral follicles of the control group. BMPR1B showed high expression in cystic follicles. Together, these results may indicate an alteration in BMPs, especially in BMP4 and BMP6, as well as in BMPR1B, which occurs early in folliculogenesis and incipiently during the development of COD, which could be a major cause of recurrence of this disease in cattle.Free Spanish abstract: A Spanish translation of this abstract is freely available at http://www.reproduction-online.org/content/early/2016/08/01/REP-15-0315/suppl/DC1.
authors.affiliation: Instituto de Ciencias Veterinarias del Litoral (ICiVET-Litoral)Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina Facultad de Ciencias VeterinariasUniversidad Nacional del Litoral, Esperanza, Santa Fe, Argentina.
authors.forename: Pablo U
authors.initials: PU
authors.lastname: Díaz
authors.affiliation: Instituto de Ciencias Veterinarias del Litoral (ICiVET-Litoral)Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina Centro Universitario GálvezUniversidad Nacional del Litoral, Gálvez, Santa Fe, Argentina.
authors.forename: Gustavo J
authors.initials: GJ
authors.lastname: Hein
authors.affiliation: Instituto de Ciencias Veterinarias del Litoral (ICiVET-Litoral)Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina Facultad de Ciencias VeterinariasUniversidad Nacional del Litoral, Esperanza, Santa Fe, Argentina.
authors.forename: Eduardo M
authors.initials: EM
authors.lastname: Belotti
authors.affiliation: Instituto de Ciencias Veterinarias del Litoral (ICiVET-Litoral)Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina.
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Rodríguez
authors.affiliation: Instituto de Ciencias Veterinarias del Litoral (ICiVET-Litoral)Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina Facultad de Ciencias VeterinariasUniversidad Nacional del Litoral, Esperanza, Santa Fe, Argentina.
authors.forename: Florencia
authors.initials: F
authors.lastname: Rey
authors.affiliation: Instituto de Ciencias Veterinarias del Litoral (ICiVET-Litoral)Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina.
authors.forename: Ayelén N
authors.initials: AN
authors.lastname: Amweg
authors.affiliation: Instituto de Ciencias Veterinarias del Litoral (ICiVET-Litoral)Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina Facultad de Ciencias VeterinariasUniversidad Nacional del Litoral, Esperanza, Santa Fe, Argentina.
authors.forename: Valentina
authors.initials: V
authors.lastname: Matiller
authors.affiliation: Instituto de Ciencias Veterinarias del Litoral (ICiVET-Litoral)Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina.
authors.forename: María E
authors.initials: ME
authors.lastname: Baravalle
authors.affiliation: Instituto de Ciencias Veterinarias del Litoral (ICiVET-Litoral)Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina Facultad de Ciencias VeterinariasUniversidad Nacional del Litoral, Esperanza, Santa Fe, Argentina.
authors.forename: Hugo H
authors.initials: HH
authors.lastname: Ortega
authors.affiliation: Instituto de Ciencias Veterinarias del Litoral (ICiVET-Litoral)Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina Facultad de Ciencias VeterinariasUniversidad Nacional del Litoral, Esperanza, Santa Fe, Argentina salvetti@fcv.unl.edu.ar.
authors.forename: Natalia R
authors.initials: NR
authors.lastname: Salvetti
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism*; D055415:Bone Morphogenetic Protein 4 / Q000235:genetics / Q000378:metabolism*; D055418:Bone Morphogenetic Protein 6 / Q000235:genetics / Q000378:metabolism*; D052004:Bone Morphogenetic Protein Receptors / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002478:Cells, Cultured; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism / Q000473:pathology; D010048:Ovarian Cysts / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006080:Ovarian Follicle / Q000378:metabolism / Q000473:pathology*; D013799:Theca Cells / Q000378:metabolism / Q000473:pathology
chemical_list: D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D055418:Bone Morphogenetic Protein 6; D052004:Bone Morphogenetic Protein Receptors