title: Efficient double-quenching of electrochemiluminescence from CdS:Eu QDs by hemin-graphene-Au nanorods ternary composite for ultrasensitive immunoassay.
abstract: A novel ternary composite of hemin-graphene-Au nanorods (H-RGO-Au NRs) with high electrocatalytic activity was synthesized by a simple method. And this ternary composite was firstly used in construction of electrochemiluminescence (ECL) immunosensor due to its double-quenching effect of quantum dots (QDs). Based on the high electrocatalytic activity of ternary complexes for the reduction of H2O2 which acted as the coreactant of QDs-based ECL, as a result, the ECL intensity of QDs decreased. Besides, due to the ECL resonance energy transfer (ECL-RET) strategy between the large amount of Au nanorods (Au NRs) on the ternary composite surface and the CdS:Eu QDs, the ECL intensity of QDs was further quenched. Based on the double-quenching effect, a novel ultrasensitive ECL immunoassay method for detection of carcinoembryonic antigen (CEA) which is used as a model biomarker analyte was proposed. The designed immunoassay method showed a linear range from 0.01 pg mL(-1) to 1.0 ng mL(-1) with a detection limit of 0.01 pg mL(-1). The method showing low detection limit, good stability and acceptable fabrication reproducibility, provided a new approach for ECL immunoassay sensing and significant prospect for practical application.
authors.affiliation: Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China.|Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan 250014, P. R. China.
authors.forename: Meirong
authors.initials: M
authors.lastname: Cui
authors.affiliation: Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000097:blood; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D005063:Europium / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006427:Hemin / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D043942:Nanotubes / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D015203:Reproducibility of Results; D018036:Selenium Compounds / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D002272:Carcinoembryonic Antigen; D018036:Selenium Compounds; D005063:Europium; D006427:Hemin; D006108:Graphite; C058667:cadmium selenide