title: Stereotactic injection of shrna GSK-3β-AAV promotes axonal regeneration after spinal cord injury.
abstract: Evidence suggested that glycogen synthase kinase-3β (GSK-3β) is involved in Nogo-66 inhibiting axonal regeneration in vitro, but its effect in vivo was poorly understood. We showed that stereotactic injection of shRNA GSK-3β-adeno associated virus (GSK-3β-AAV) diminished syringomyelia and promoted axonal regeneration after spinal cord injury (SCI), using stereotactic injection of shRNA GSK-3β-AAV (tested with Western blotting and RT-PCR) into the sensorimotor cortex of rats with SCI and by the detection of biotin dextran amine (BDA)-labeled axonal regeneration. We also determined the right position to inject into the sensorimotor cortex. Our findings consolidate the hypothesis that downregulation of GSK-3β promotes axonal regeneration after SCI.
authors.affiliation: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.|Department of Neurosurgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Yu-Chao
authors.initials: YC
authors.lastname: Zuo
authors.affiliation: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. mozhuoxiong@163.com.
authors.forename: Nan-Xiang
authors.initials: NX
authors.lastname: Xiong
authors.affiliation: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. hyzhao750@sina.com.
authors.forename: Hong-Yang
authors.initials: HY
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects / Q000378:metabolism; D000229:Dependovirus / Q000235:genetics; D000071679:Glycogen Synthase Kinase 3 beta / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009416:Nerve Regeneration / Q000235:genetics*; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D051381:Rats; D066191:Sensorimotor Cortex / Q000187:drug effects / Q000473:pathology; D013119:Spinal Cord Injuries / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D013595:Syringomyelia / Q000235:genetics* / Q000473:pathology / Q000628:therapy
chemical_list: D034741:RNA, Small Interfering; D000071679:Glycogen Synthase Kinase 3 beta
keywords: myelin proteins; neurites; protein-serine-threonine kinases; wound healing