title: Isolation and characterization of butanol-tolerant Staphylococcus aureus.
abstract: OBJECTIVE
  A new solvent-tolerant species, Staphylococcus aureus, was isolated and characterized during the screening of butanol-tolerant microorganisms.
  
  
  RESULTS
  Three isolates of S. aureus were obtained as contaminants during improvement of butanol tolerance of E. coli K12. Their cell dry weights were 135 % that of K12 in the absence of butanol stress. S. aureus had a growth advantage over K12 when cultured with various concentrations of butanol. It can tolerate up to 3 % (v/v) butanol, while most solventogenic bacteria can tolerate only 2 % (v/v) butanol. The addition of 10-20 g glucose/l enhanced its butanol tolerance. The relative cell biomass of the S. aureus was 71-306 % that of E. coli under 5.5-10 % (v/v) ethanol stress, indicating ethanol resistance.
  
  
  CONCLUSIONS
  This is the first study to observe butanol-tolerant S. aureus. As this organism can be genetically manipulated, it could have a wide array of applications.
authors.affiliation: R&D Center for Petrochemical Technology, Tianjin University, Tianjin, 300072, China.|Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
authors.forename: Junyan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: R&D Center for Petrochemical Technology, Tianjin University, Tianjin, 300072, China.|Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
authors.forename: Suzhen
authors.initials: S
authors.lastname: Huang
authors.affiliation: R&D Center for Petrochemical Technology, Tianjin University, Tianjin, 300072, China. myy@tju.edu.cn.|Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, 300072, China. myy@tju.edu.cn.|Key Laboratory for Green Chemical Technology of Ministry of Education, R&D Center for Petrochemical Technology, Tianjin University, Tianjin, 300072, China. myy@tju.edu.cn.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: R&D Center for Petrochemical Technology, Tianjin University, Tianjin, 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, 300072, China.|Key Laboratory for Green Chemical Technology of Ministry of Education, R&D Center for Petrochemical Technology, Tianjin University, Tianjin, 300072, China.
authors.forename: Minhua
authors.initials: M
authors.lastname: Zhang
authors.affiliation: R&D Center for Petrochemical Technology, Tianjin University, Tianjin, 300072, China.
authors.forename: Shaolan
authors.initials: S
authors.lastname: Zou
mesh_terms: D000440:Butanols / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D048168:Escherichia coli K12 / Q000254:growth & development; D005947:Glucose / Q000494:pharmacology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*
chemical_list: D000440:Butanols; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005947:Glucose
keywords: Butanol; Ethanol; Glucose; Staphylococcus aureus; Tolerance