title: Immunotoxic Effect of Low-Dose Methylmercury Is Negligible in Mouse Models of Ovalbumin or Mite-Induced Th2 Allergy.
abstract: Methylmercury (MeHg) is one of the most toxic environmental pollutants and presents a serious hazard to health worldwide. Although the adverse effects of MeHg, including neurotoxicity, have been studied, its effects on immune function, in particular the immune response, remain unclear. This study examined the effects of low-dose MeHg on immune responses in mice. Mice were orally immunized with ovalbumin (OVA) or subcutaneously injected with mite extract to induce a T-helper 2 (Th2) allergic response. They were then exposed to MeHg (0, 0.02, 1.0, or 5.0 mg·kg(-1)·d(-1)). Immunization with oral OVA or subcutaneous mite extract increased serum levels of OVA-specific immunoglobulin (Ig) E (OVA-IgE), OVA-IgG1, interleukin (IL)-4, and IL-13, and total IgE, total IgG, and IL-13 when compared with levels in non-immunized mice. However, no interferon (IFN)-γ was detected. By contrast, serum levels of OVA-IgE, OVA-IgG1, IL-4, and IL-13, or total IgE, total IgG, and IL-13 in Th2 allergy model mice subsequently treated with MeHg were no higher than those in MeHg-untreated mice. These results suggest that MeHg exposure has no adverse effects on Th2 immune responses in antigen-immunized mice.
authors.affiliation: Department of Public Health, School of Pharmacy, Kitasato University.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Nakamura
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Takanezawa
authors.forename: Yuka
authors.initials: Y
authors.lastname: Sone
authors.forename: Shimpei
authors.initials: S
authors.lastname: Uraguchi
authors.forename: Kou
authors.initials: K
authors.lastname: Sakabe
authors.forename: Masako
authors.initials: M
authors.lastname: Kiyono
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D040002:Dermatophagoides pteronyssinus / Q000276:immunology*; D004195:Disease Models, Animal; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008297:Male; D008767:Methylmercury Compounds / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D016207:Cytokines; D007074:Immunoglobulin G; D008767:Methylmercury Compounds; D007073:Immunoglobulin E; D010047:Ovalbumin