title: Chemoprotective action of lotus seedpod procyanidins on oxidative stress in mice induced by extremely low-frequency electromagnetic field exposure.
abstract: With the increasing use of electromagnetic technology, the effects of extremely low-frequency electromagnetic fields (ELF-EMF) on biological systems, central neurotransmitter systems, and human health have attracted extensive attention worldwide. In this study, lotus seedpod procyanidins (LSPCs) were evaluated for their protective effects on ELF-EMF induced oxidative stress injury in mice. Sixty male ICR mice were used for the experiment. The mice were randomly divided into five equal groups. The control group did not receive LSPCs or ELF-EMF but orally received normal saline. The ELF-EMF group received ELF-EMF exposure plus normal saline orally. The other three groups received ELF-EMF exposure plus LSPCs orally (60, 90, or 120mg kg(-1).bw, respectively). Each group exposed to ELF-EMF at 8 mT, 4h day(-1) for 28 consecutive days after administration daily of LSPCs or normal saline to mice for 15 consecutive days with the exception of the control group. Thereafter, blood and cerebral cortex of the mice were analyzed for antioxidant indices, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione-S-transferase (GST) and malondialdehyde (MDA). LSPCs administration at different doses significantly inhibited oxidative stress damage of mice induced by ELF-EMF. LSPCs treatment augmented SOD, CAT, GSH-Px, GR and GST activity. Furthermore, administration significantly lowered MDA level in LSPCs treatment groups LSPCs. All results indicated LSPCs can effectively prevent oxidative stress injury induced by ELF-EMF exposure, which may be related to its ability of scavenging free radicals and stimulating antioxidant enzyme activity.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Luo
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Chen
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: dyq101@mail.ujs.edu.cn.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Duan
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Duan
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: zhanghh@mail.ujs.edu.cn.
authors.forename: Haihui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: He
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Chunchun
authors.initials: C
authors.lastname: Yin
authors.affiliation: Institute of Medicinal Plants, Chinese Academy of Medical Sciences, Beijing 100193, China.
authors.forename: Guibo
authors.initials: G
authors.lastname: Sun
authors.affiliation: Institute of Medicinal Plants, Chinese Academy of Medical Sciences, Beijing 100193, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Sun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D044946:Biflavonoids / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002392:Catechin / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D004574:Electromagnetic Fields; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008813:Mice, Inbred ICR; D031653:Nelumbo / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D044945:Proanthocyanidins / Q000494:pharmacology*; D020011:Protective Agents; D012639:Seeds / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D044945:Proanthocyanidins; D020011:Protective Agents; C017674:procyanidin; D008315:Malondialdehyde; D002392:Catechin
keywords: Antioxidant enzyme; Pathological observation; Preventive effects; Ros