title: Mesenchymal stem cells attenuate ischemia-reperfusion injury after prolonged cold ischemia in a mouse model of lung transplantation: a preliminary study.
abstract: OBJECTIVE
  Mesenchymal stem cells (MSCs) suppress inflammation and immune responses. We conducted this study to find out if MSCs attenuate ischemia-reperfusion injury in a mouse model of lung transplantation.
  
  
  METHODS
  C57BL/6J mouse lungs perfused with low-potassium dextran glucose solution were preserved at 4 °C for 18 h. Human MSCs were slowly injected into the left pulmonary artery of the lung grafts, and orthotopic left lung transplantation was then performed. The lung isografts were reperfused for 6 h, and bronchoalveolar lavage fluid (BALF) from the left lung graft was collected. We measured the protein concentration, cell count, and proinflammatory cytokine concentrations in the BALF.
  
  
  RESULTS
  The protein concentration and cell count in the BALF were significantly lower in the MSC-administered grafts than in the PBS-administered controls. Concentrations of proinflammatory cytokines, including IL-1β, IL-17A, and TNF-α, in BALF tended to be lower in the MSC-administered grafts than in the controls, but the difference was not significant.
  
  
  CONCLUSIONS
  The pre-transplant administration of MSCs via the pulmonary artery of the lung graft attenuated ischemia-reperfusion injury after prolonged cold ischemia in this mouse model of lung transplantation.
authors.affiliation: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai, 980-8575, Japan. tatsuaki_watanabe@yahoo.co.jp.
authors.forename: Tatsuaki
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai, 980-8575, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hoshikawa
authors.affiliation: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai, 980-8575, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Ishibashi
authors.affiliation: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai, 980-8575, Japan.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai, 980-8575, Japan.
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Notsuda
authors.affiliation: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai, 980-8575, Japan.
authors.forename: Yui
authors.initials: Y
authors.lastname: Watanabe
authors.affiliation: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai, 980-8575, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Noda
authors.affiliation: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai, 980-8575, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kanehira
authors.affiliation: Department of Respiratory Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Ohkouchi
authors.affiliation: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai, 980-8575, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.affiliation: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai, 980-8575, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Okada
mesh_terms: D000818:Animals; D003080:Cold Temperature / Q000009:adverse effects; D007511:Ischemia / Q000209:etiology / Q000628:therapy*; D008168:Lung / Q000098:blood supply*; D016040:Lung Transplantation; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D011651:Pulmonary Artery; D015427:Reperfusion Injury / Q000209:etiology / Q000628:therapy*; D013997:Time Factors
keywords: Ischemia–reperfusion injury; Lung transplantation; Mesenchymal stem cells