title: Antiproliferative Effect of Synadenium grantii Hook f. stems (Euphorbiaceae) and a Rare Phorbol Diterpene Ester.
abstract: Synadenium grantii is frequently used for the treatment of various diseases such as allergies, gastric disorders, and especially cancer. The aim of this study was to evaluate the possible antiproliferative potential of the methanol extract, fractions, and pure compounds from the stems of S grantii Phytochemical analysis was carried out by conventional chromatographic techniques, and the antiproliferative activity was analyzed using the sulforhodamine B assay and an MTT-based assay. Nonpolar fraction and its subfractions from the stems of S grantii exhibited promising cytostatic effect against several human tumor cell lines (glioma, breast, kidney, and lung), with total grown inhibition values ranging from 0.37 to 2.9 μg/mL. One of the active principles of this plant was identified as a rare phorbol diterpene ester, denoted as 3,4,12,13-tetraacetylphorbol-20-phenylacetate. This compound demonstrated antiproliferative activity against glioma, kidney, lung, and triple-negative breast cancer cell lines. These results demonstrate that S grantii stems produce active principles with relevant antiproliferative potential.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas and Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí-UNIVALI, Itajaí, Santa Catarina, Brazil adriicampos@hotmail.com.
authors.forename: Adriana
authors.initials: A
authors.lastname: Campos
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas-Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.
authors.forename: Débora Barbosa
authors.initials: DB
authors.lastname: Vendramini-Costa
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas-Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.
authors.forename: Giovanna Barbarini
authors.initials: GB
authors.lastname: Longato
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas and Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí-UNIVALI, Itajaí, Santa Catarina, Brazil.
authors.forename: Tailyn
authors.initials: T
authors.lastname: Zermiani
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas-Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.
authors.forename: Ana Lúcia Tasca Gois
authors.initials: AL
authors.lastname: Ruiz
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas-Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.
authors.forename: João Ernesto
authors.initials: JE
authors.lastname: de Carvalho
authors.affiliation: Centro de Investigación del Cáncer (CSIC-USAL), Salamanca, Spain.
authors.forename: Atanasio
authors.initials: A
authors.lastname: Pandiella
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas and Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí-UNIVALI, Itajaí, Santa Catarina, Brazil.
authors.forename: Valdir
authors.initials: V
authors.lastname: Cechinel Filho
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019665:Euphorbiaceae; D006801:Humans; D010703:Phorbol Esters / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D018547:Plant Stems
chemical_list: C000625973:3,4,12,13-tetraacetylphorbol-20-phenylacetate; D000970:Antineoplastic Agents; D010703:Phorbol Esters; D010936:Plant Extracts
keywords: Synadenium grantii; antiproliferative action; nonpolar fraction; phorbol ester