title: DNA Extraction Protocols for Whole-Genome Sequencing in Marine Organisms.
abstract: The marine environment harbors a large proportion of the total biodiversity on this planet, including the majority of the earths' different phyla and classes. Studying the genomes of marine organisms can bring interesting insights into genome evolution. Today, almost all marine organismal groups are understudied with respect to their genomes. One potential reason is that extraction of high-quality DNA in sufficient amounts is challenging for many marine species. This is due to high polysaccharide content, polyphenols and other secondary metabolites that will inhibit downstream DNA library preparations. Consequently, protocols developed for vertebrates and plants do not always perform well for invertebrates and algae. In addition, many marine species have large population sizes and, as a consequence, highly variable genomes. Thus, to facilitate the sequence read assembly process during genome sequencing, it is desirable to obtain enough DNA from a single individual, which is a challenge in many species of invertebrates and algae. Here, we present DNA extraction protocols for seven marine species (four invertebrates, two algae, and a marine yeast), optimized to provide sufficient DNA quality and yield for de novo genome sequencing projects.
authors.affiliation: Department of Marine Sciences, University of Gothenburg, Strömstad, Sweden. marina.panova@gu.se.
authors.forename: Marina
authors.initials: M
authors.lastname: Panova
authors.affiliation: Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Aronsson
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
authors.forename: R Andrew
authors.initials: RA
authors.lastname: Cameron
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Dahl
authors.affiliation: Department of Marine Sciences, University of Gothenburg, Strömstad, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Godhe
authors.affiliation: Department of Marine Sciences, University of Gothenburg, Strömstad, Sweden.
authors.forename: Ulrika
authors.initials: U
authors.lastname: Lind
authors.affiliation: Department of Marine Sciences, University of Gothenburg, Strömstad, Sweden.
authors.forename: Olga
authors.initials: O
authors.lastname: Ortega-Martinez
authors.affiliation: Department of Marine Sciences, University of Gothenburg, Strömstad, Sweden.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Pereyra
authors.affiliation: Department of Marine Sciences, University of Gothenburg, Strömstad, Sweden.
authors.forename: Sylvie V M
authors.initials: SV
authors.lastname: Tesson
authors.affiliation: Department of Marine Sciences, University of Gothenburg, Strömstad, Sweden.
authors.forename: Anna-Lisa
authors.initials: AL
authors.lastname: Wrange
authors.affiliation: Department of Marine Sciences, University of Gothenburg, Strömstad, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Blomberg
authors.affiliation: Department of Marine Sciences, University of Gothenburg, Strömstad, Sweden.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Johannesson
mesh_terms: D000818:Animals; D059001:Aquatic Organisms / Q000145:classification / Q000235:genetics*; D044822:Biodiversity; D023281:Genomics; D007448:Invertebrates / Q000145:classification / Q000235:genetics; D008386:Marine Biology / Q000379:methods*; D000459:Phaeophyceae / Q000145:classification / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D001468:Thoracica / Q000145:classification / Q000235:genetics; D014871:Water Microbiology; D015003:Yeasts / Q000145:classification / Q000235:genetics
keywords: Barnacle Balanus; Brittle star Amphiura; Brown alga Fucus; Diatom Skeletonema; Gastropod Littorina; Genomic DNA extraction; Isopod Idotea; Marine yeast Debaryomyces