title: Proteomic Analysis of Pachytene Spermatocytes of Sterile Hybrid Male Mice.
abstract: Incompatibilities in interspecific hybrids, such as reduced hybrid fertility and lethality, are common features resulting from reproductive isolation that lead to speciation. Subspecies crosses of house mice produce offspring in which one sex is infertile or absent, yet the molecular mechanisms of hybrid sterility are poorly understood. In this study, we observed extensive asynapsis of chromosomes and disturbance of the sex body in pachytene spermatocytes of sterile F1 males (PWK/Ph female × C57BL/6J male). We report the high-confidence identification of 4005 proteins in the pachytene spermatocytes of fertile F1 males (PWK/Ph male × C57BL/6J female) and sterile F1 males (PWK/Ph female × C57BL/6J male), of which 215 were upregulated and 381 were downregulated. Bioinformatics analysis of the proteome led to the identification of 43 and 59 proteins known to be essential for male meiosis and spermatogenesis in mice, respectively. Characterization of the proteome of pachytene spermatocytes associated with hybrid male sterility provides an inventory of proteins that is useful for understanding meiosis and the mechanisms of hybrid male infertility.
authors.affiliation: Shanghai Key Laboratory for Reproductive Medicine, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, State Key Laboratory of Reproductive Medicine, Key Laboratory of Model Animal Research, Animal Core Facility, Nanjing Medical University, Nanjing, China.
authors.forename: Yueshuai
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Life Science and Technology, Southwest University of Science and Technology, Mianyang, Sichuan, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Liu
authors.affiliation: Engineering College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, Henan, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Engineering College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, Henan, China.
authors.forename: Gendi
authors.initials: G
authors.lastname: Song
authors.affiliation: Department of Histology and Embryology, State Key Laboratory of Reproductive Medicine, Key Laboratory of Model Animal Research, Animal Core Facility, Nanjing Medical University, Nanjing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Shanghai Key Laboratory for Reproductive Medicine, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Hefeng
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Histology and Embryology, State Key Laboratory of Reproductive Medicine, Key Laboratory of Model Animal Research, Animal Core Facility, Nanjing Medical University, Nanjing, China guo_xuejiang@njmu.edu.cn.
authors.forename: Xuejiang
authors.initials: X
authors.lastname: Guo
authors.affiliation: Shanghai Key Laboratory for Reproductive Medicine, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China sunfei@shsmu.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D007248:Infertility, Male / Q000378:metabolism*; D008297:Male; D008540:Meiosis / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D040901:Proteomics; D013090:Spermatocytes / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000378:metabolism
keywords: hybrid male sterility; meiosis; pachytene spermatocytes; spermatogenesis