title: Nanoscale investigation of the interaction of colistin with model phospholipid membranes by Langmuir technique, and combined infrared and force spectroscopies.
abstract: Colistin (Polymyxin E), an antimicrobial peptide, is increasingly put forward as salvage for severe multidrug-resistant infections. Unfortunately, colistin is potentially toxic to mammalian cells. A better understanding of the interaction with specific components of the cell membranes may be helpful in controlling the factors that may enhance toxicity. Here, we report a physico-chemical study of model phospholipid (PL) mono- and bilayers exposed to colistin at different concentrations by Langmuir technique, atomic force microscopy (AFM) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The effect of colistin on chosen PL monolayers was examined. Insights into the topographical and elastic changes in the PL bilayers within time after peptide injection are presented via AFM imaging and force spectra. Finally, changes in the PL bilayers' ATR-FTIR spectra as a function of time within three bilayer compositions, and the influence of colistin on their spectral fingerprint are examined together with the time-evolution of the Amide II and νCO band integrated intensity ratios. Our study reveals a great importance in the role of the PL composition as well as the peptide concentration on the action of colistin on PL model membranes.
authors.affiliation: Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME, UMR 7564, Villers-lès-Nancy F-54600, France; CNRS, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME, UMR 7564, Villers-lès- Nancy F-54600, France; Université de Lorraine, Structure et Réactivité des Systèmes Moléculaires Complexes, SRSMC, UMR7565, Vandœuvre-lès-Nancy, cedex, F-54506, France.
authors.forename: Oona
authors.initials: O
authors.lastname: Freudenthal
authors.affiliation: Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME, UMR 7564, Villers-lès-Nancy F-54600, France; CNRS, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME, UMR 7564, Villers-lès- Nancy F-54600, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Quilès
authors.affiliation: Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME, UMR 7564, Villers-lès-Nancy F-54600, France; CNRS, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME, UMR 7564, Villers-lès- Nancy F-54600, France. Electronic address: gregory.francius@univ-lorraine.fr.
authors.forename: Grégory
authors.initials: G
authors.lastname: Francius
authors.affiliation: Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, ul. R. Ingardena 3, 30-060 Krakow, Poland; Université de Lorraine, Structure et Réactivité des Systèmes Moléculaires Complexes, SRSMC, UMR7565, Vandœuvre-lès-Nancy, cedex, F-54506, France.
authors.forename: Kamila
authors.initials: K
authors.lastname: Wojszko
authors.affiliation: Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, ul. R. Ingardena 3, 30-060 Krakow, Poland; Université de Lorraine, Structure et Réactivité des Systèmes Moléculaires Complexes, SRSMC, UMR7565, Vandœuvre-lès-Nancy, cedex, F-54506, France.
authors.forename: Marcelina
authors.initials: M
authors.lastname: Gorczyca
authors.affiliation: Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, ul. R. Ingardena 3, 30-060 Krakow, Poland; Université de Lorraine, Structure et Réactivité des Systèmes Moléculaires Complexes, SRSMC, UMR7565, Vandœuvre-lès-Nancy, cedex, F-54506, France.
authors.forename: Beata
authors.initials: B
authors.lastname: Korchowiec
authors.affiliation: Université de Lorraine, Structure et Réactivité des Systèmes Moléculaires Complexes, SRSMC, UMR7565, Vandœuvre-lès-Nancy, cedex, F-54506, France; CNRS, Structure et Réactivité des Systèmes Moléculaires Complexes, SRSMC, UMR7565, Vandœuvre-lès-Nancy, cedex, F-54506, France.
authors.forename: Ewa
authors.initials: E
authors.lastname: Rogalska
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D003091:Colistin / Q000737:chemistry*; D004548:Elasticity; D008051:Lipid Bilayers / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D053835:Unilamellar Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; C017251:1,2-oleoylphosphatidylcholine; D003091:Colistin
keywords: ATR-FTIR; Antimicrobial peptide; Atomic force microscopy; Colistin; Compression isotherms; Monolayer; Supported lipid bilayer