title: Evaluation of xylitol production using corncob hemicellulosic hydrolysate by combining tetrabutylammonium hydroxide extraction with dilute acid hydrolysis.
abstract: In this paper, we produced hemicellulosic hydrolysate from corncob by tetrabutylammonium hydroxide (TBAH) extraction and dilute acid hydrolysis combined, further evaluating the feasibility of the resultant corncob hemicellulosic hydrolysate used in xylitol production by Candida tropicalis. Optimized conditions for corncob hemicellulose extraction by TBAH was obtained via response surface methodology: time of 90min, temperature of 60°C, liquid/solid ratio of 12 (v/w), and TBAH concentration of 55%, resulting in a hemicellulose extraction of 80.07% under these conditions. The FT-IR spectrum of the extracted corncob hemicellulose is consistent with that of birchwood hemicellulose and exhibits specific absorbance of hemicelluloses at 1380, 1168, 1050, and 900cm(-1). In addition, we found that C. tropicalis can ferment the resulting corncob hemicellulosic hydrolysate with pH adjustment and activated charcoal treatment leading to a high xylitol yield and productivity of 0.77g/g and 2.45g/(Lh), respectively.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China. Electronic address: hhjia@njtech.edu.cn.
authors.forename: Honghua
authors.initials: H
authors.lastname: Jia
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Shao
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhong
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Hengxiang
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
mesh_terms: D041022:Candida tropicalis / Q000378:metabolism; D005240:Feasibility Studies; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D011134:Polysaccharides / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D014993:Xylitol / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D003313:Zea mays / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D000644:Quaternary Ammonium Compounds; C007916:hemicellulose; C009405:tetrabutylammonium; D014993:Xylitol
keywords: Candida tropicalis; Corncob; Hemicellulosic hydrolysate; Tetrabutylammonium hydroxide; Xylitol