title: Membrane Protein Production in Lactococcus lactis for Functional Studies.
abstract: Due to their unique properties, expression and study of membrane proteins in heterologous systems remains difficult. Among the bacterial systems available, the Gram-positive lactic bacterium, Lactococcus lactis, traditionally used in food fermentations, is nowadays widely used for large-scale production and functional characterization of bacterial and eukaryotic membrane proteins. The aim of this chapter is to describe the different possibilities for the functional characterization of peripheral or intrinsic membrane proteins expressed in Lactococcus lactis.
authors.affiliation: Laboratoire de Physiologie Cellulaire et Végétale, CNRS (UMR-5168)/CEA/INRA (UMR1417)/Université Grenoble Alpes, BIG, CEA, Grenoble, France.
authors.forename: Daphne
authors.initials: D
authors.lastname: Seigneurin-Berny
authors.affiliation: Medical Research Council, Mitochondrial Biology Unit, Hills Road, Cambridge, CB2 2XY, UK.
authors.forename: Martin S
authors.initials: MS
authors.lastname: King
authors.affiliation: Laboratoire de Physiologie Cellulaire et Végétale, CNRS (UMR-5168)/CEA/INRA (UMR1417)/Université Grenoble Alpes, BIG, CEA, Grenoble, France.
authors.forename: Emiline
authors.initials: E
authors.lastname: Sautron
authors.affiliation: Laboratoire de Physiologie Cellulaire et Végétale, CNRS (UMR-5168)/CEA/INRA (UMR1417)/Université Grenoble Alpes, BIG, CEA, Grenoble, France.
authors.forename: Lucas
authors.initials: L
authors.lastname: Moyet
authors.affiliation: Laboratoire de Chimie et Biologie des Métaux, CNRS (UMR-5249)/CEA/Université Grenoble Alpes, BIG, CEA, Grenoble, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Catty
authors.affiliation: Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris Saclay, Gif-sur-Yvette, France.
authors.forename: François
authors.initials: F
authors.lastname: André
authors.affiliation: Laboratoire de Physiologie Cellulaire et Végétale, CNRS (UMR-5168)/CEA/INRA (UMR1417)/Université Grenoble Alpes, BIG, CEA, Grenoble, France.
authors.forename: Norbert
authors.initials: N
authors.lastname: Rolland
authors.affiliation: Medical Research Council, Mitochondrial Biology Unit, Hills Road, Cambridge, CB2 2XY, UK.
authors.forename: Edmund R S
authors.initials: ER
authors.lastname: Kunji
authors.affiliation: Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris Saclay, Gif-sur-Yvette, France. annie.frelet-barrand@femto-st.fr.|FEMTO-ST Institute, UMR CNRS 6174, University of Bourgogne Franche-Comte, Besançon, France. annie.frelet-barrand@femto-st.fr.
authors.forename: Annie
authors.initials: A
authors.lastname: Frelet-Barrand
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D013294:Lactococcus lactis / Q000235:genetics / Q000254:growth & development*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins
keywords: Expression; Lactococcus lactis; Membrane proteins; Transport assays