title: Arabidopsis JINGUBANG Is a Negative Regulator of Pollen Germination That Prevents Pollination in Moist Environments.
abstract: The molecular mechanism of pollen germination and pollen tube growth has been revealed in detail during the last decade, while the mechanism that suspends pollen grains in a dormant state is largely unclear. Here, we identified theJINGUBANG(JGB) gene by screening pollen-specific genes for those that are unnecessary for pollen germination. We showed that the pollen of thejgbloss-of-function mutant exhibited hyperactive germination in sucrose-only medium and inside the anther, while this phenotype was rescued by the transgenic expression ofJGBinjgbplants. JGB contains seven WD40 repeats and is highly conserved in flowering plants. Overexpression ofJGBinhibits pollen germination. These results indicate that JGB is a novel negative regulator of pollen germination. In addition, we found that jasmonic acid (JA) abundance was significantly elevated injgbpollen, while exogenous application of methyl jasmonate rescued the inhibition of pollen germination in plants overexpressingJGBBased on the molecular features of JGB and on the finding that it interacts with a known JA biosynthesis-related transcription factor, TCP4, we propose that JGB, together with TCP4, forms a regulatory complex that controls pollen JA synthesis, ensuring pollination in moist environments.
authors.affiliation: Key Laboratory of Ministry of Education for Cell Proliferation and Differentiation, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Yan
authors.identifier: http://orcid.org/0000-0002-5022-2053
authors.initials: Y
authors.lastname: Ju
authors.affiliation: Key Laboratory of Ministry of Education for Cell Proliferation and Differentiation, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Liang
authors.identifier: http://orcid.org/0000-0002-1954-1179
authors.initials: L
authors.lastname: Guo
authors.affiliation: Key Laboratory of Ministry of Education for Cell Proliferation and Differentiation, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Qiang
authors.identifier: http://orcid.org/0000-0001-5871-2519
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Key Laboratory of Ministry of Education for Cell Proliferation and Differentiation, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ma
authors.affiliation: Key Laboratory of Ministry of Education for Cell Proliferation and Differentiation, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Qiao-Yun
authors.initials: QY
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Ministry of Education for Cell Proliferation and Differentiation, College of Life Sciences, Peking University, Beijing 100871, China zhang.q@water.pku.edu.cn sodmergn@pku.edu.cn.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Ministry of Education for Cell Proliferation and Differentiation, College of Life Sciences, Peking University, Beijing 100871, China zhang.q@water.pku.edu.cn sodmergn@pku.edu.cn.
authors.lastname: Sodmergen