title: Radio-frequency plasma polymerized biodegradable carrier for in vivo release of cis-platinum.
abstract: A low pressure plasma process based on plasma deposition has been used to develop a drug delivery strategy. In this study, a drug delivery system based on different layers of plasma co-polymerized Poly ε-caprolactone-Polyethylene glycol (PCL-PEG) co-polymers was deposited on biocompatible substrates. Cis-platinum (118 μgm/cm2) was used as an anti-cancer drug and incorporated for local delivery of the chemotherapeutic agent. The co-polymer layers and their interaction with cancer cells were analyzed by scanning electron microscopy. Our study showed that the plasma-PCL-PEG coated cellophane membranes, in which the drug, was included did not modify the flexibility and appearance of the membranes. This system was actively investigated as an alternative method of controlling localized delivery of drug in vivo. The loading of the anti-cancer drug was investigated by UV-VIS spectroscopy and its release from plasma deposited implants against BALB/c mice liver tissues were analyzed through histological examination and apoptosis by TUNEL assay. The histological examination of liver tissues revealed that when the plasma-modified membranes encapsulated the cis-platinum, the Glisson's capsule and liver parenchyma were damaged. In all cases, inflammatory tissues and fibrosis cells were observed in contact zones between the implant and the liver parenchyma. In conclusion, low pressure plasma deposited uniform nano-layers of the co-polymers can be used for controlled release of the drug in vivo.
authors.affiliation: Sorbonne Universités, UPMC Université Paris 6, CNRS, Laboratoire Interfaces et Systèmes Electrochimiques, 75005, Paris, France.|Current address: Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, 23508, VA, USA.
authors.forename: Sudhir
authors.initials: S
authors.lastname: Bhatt
authors.affiliation: Sorbonne Paris Cité Universités, UMR Université Paris 7, INSERM U965 Carcinose, Angiogenèse et Recherche Translationnelle, L'Hôpital Lariboisière, 75010, Paris, France.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Valamanesh
authors.affiliation: Sorbonne Universités, UPMC Université Paris 6, CNRS, Laboratoire Interfaces et Systèmes Electrochimiques, 75005, Paris, France.
authors.forename: Jerome
authors.initials: J
authors.lastname: Pulpytel
authors.affiliation: Sorbonne Paris Cité Universités, UMR Université Paris 7, INSERM U965 Carcinose, Angiogenèse et Recherche Translationnelle, L'Hôpital Lariboisière, 75010, Paris, France.
authors.forename: Rea
authors.initials: R
authors.lastname: Lo Dico
authors.affiliation: Sorbonne Universités, UPMC Université Paris 6, CNRS, Laboratoire Interfaces et Systèmes Electrochimiques, 75005, Paris, France.
authors.forename: Aliby
authors.initials: A
authors.lastname: Baiyukha
authors.affiliation: Sorbonne Paris Cité Universités, UMR Université Paris 7, INSERM U965 Carcinose, Angiogenèse et Recherche Translationnelle, L'Hôpital Lariboisière, 75010, Paris, France.
authors.forename: Iman
authors.initials: I
authors.lastname: Al-Dybiat
authors.affiliation: Sorbonne Paris Cité Universités, UMR Université Paris 7, INSERM U965 Carcinose, Angiogenèse et Recherche Translationnelle, L'Hôpital Lariboisière, 75010, Paris, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Pocard
authors.affiliation: Sorbonne Universités, UPMC Université Paris 6, CNRS, Laboratoire Interfaces et Systèmes Electrochimiques, 75005, Paris, France.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Arefi-Khonsari
authors.affiliation: Sorbonne Paris Cité Universités, UMR Université Paris 7, INSERM U965 Carcinose, Angiogenèse et Recherche Translationnelle, L'Hôpital Lariboisière, 75010, Paris, France.
authors.forename: Massoud
authors.initials: M
authors.lastname: Mirshahi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D063566:Biodegradable Plastics / Q000737:chemistry / Q000528:radiation effects; D002208:Caproates / Q000737:chemistry / Q000528:radiation effects; D002476:Cellophane / Q000737:chemistry / Q000528:radiation effects; D002945:Cisplatin / Q000008:administration & dosage*; D003692:Delayed-Action Preparations / Q000008:administration & dosage; D004337:Drug Carriers / Q000737:chemistry* / Q000528:radiation effects; D004343:Drug Implants; D005260:Female; D007783:Lactones / Q000737:chemistry / Q000528:radiation effects; D008099:Liver / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D011092:Polyethylene Glycols / Q000737:chemistry / Q000528:radiation effects; D058105:Polymerization / Q000528:radiation effects*; D011108:Polymers / Q000737:chemistry / Q000528:radiation effects; D011846:Radio Waves; D013057:Spectrum Analysis
chemical_list: D000970:Antineoplastic Agents; D063566:Biodegradable Plastics; D002208:Caproates; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004343:Drug Implants; D007783:Lactones; D011108:Polymers; D011092:Polyethylene Glycols; C121056:caprolactone; D002476:Cellophane; D002945:Cisplatin
keywords: anti-cancer drug; biodegradable coatings; in vivo drug delivery system; plasma polymers; targeted therapy