title: Use of magnetic resonance imaging to assess ovarian maturation in live Rhinogobio ventralis (Sauvage & Dabry de Thiersant, 1874).
abstract: The aim of this study is to evaluate the application of magnetic resonance imaging (MRI) to assess ovarian maturation in live female Rhinogobio ventralis (Sauvage & Dabry de Thiersant, 1874). The fish were randomly collected from the Jiangjin area of the Yangtze River between January and April 2014. Magnetic resonance imaging was performed using a 3.0 T clinical MRI scanner with a brain coil and two pulse sequences (IDEAL and 3D CUBE) were employed. Magnetic resonance and histologic images at different stages of ovarian maturation (I-IV) were acquired. An empirical equation (y = -0.1 + 1.56 × x) was derived by traditional method to describe the relationship between the gonadosomatic index (y) and the percentage volume of the ovary (x). A significant correlation (R(2) = 0.977, P < 0.01, N = 53) was found between measurements of the percentage volume of the ovary by MRI and traditional methods. The research findings suggested that MRI was a reliable, rapid, and noninvasive method to assess stages of ovarian maturity in female R. ventralis.
authors.affiliation: Key Laboratory of Ecological Impacts of Hydraulic-projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan, People's Republic of China; Faculty of Resources and Environmental Science, Hubei University, Wuhan, People's Republic of China; Regional Development and Environmental Response, Key Laboratory of Hubei Province, Wuhan, People's Republic of China. Electronic address: gali3721@gmail.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Radiology, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
authors.forename: Junwu
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Radiology, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
authors.forename: Chengdong
authors.initials: C
authors.lastname: Peng
authors.affiliation: Department of Radiology, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: Ziheng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Ecological Impacts of Hydraulic-projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan, People's Republic of China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Xie
authors.affiliation: Key Laboratory of Ecological Impacts of Hydraulic-projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan, People's Republic of China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Qin
mesh_terms: D000818:Animals; D005260:Female; D005399:Fishes / Q000033:anatomy & histology / Q000502:physiology*; D008279:Magnetic Resonance Imaging / Q000662:veterinary*; D010053:Ovary / Q000000981:diagnostic imaging* / Q000502:physiology*; D012741:Sexual Maturation / Q000502:physiology*
keywords: Assessment; Magnetic resonance imaging; Ovarian maturation; Rhinogobio ventralis