title: Running for REST: Physical activity attenuates neuroinflammation in the hippocampus of aged mice.
abstract: Exercise improves mental health and synaptic function in the aged brain. However, the molecular mechanisms involved in exercise-induced healthy brain aging are not well understood. Evidence supports the role of neurogenesis and neurotrophins in exercise-induced neuroplasticity. The gene silencing transcription factor neuronal RE1-silencing transcription factor (REST)/neuron-restrictive silencer factor (NRSF) and an anti-inflammatory role of exercise are also candidate mechanisms. We evaluate the effect of 8weeks of physical activity on running wheels (RW) on motor and depressive-like behavior and hippocampal gene expression of brain-derived neurotrophic factor (BDNF), REST, and interleukins IL-1β and IL-10 of adult and aged C57BL/6 mice. The aged animals exhibited impaired motor function and a depressive-like behavior: decreased mobility in the RW and open field and severe immobility in the tail suspension test. The gene expression of REST, IL-1β, and IL-10 was increased in the hippocampus of aged mice. Physical activity was anxiolytic and antidepressant and improved motor behavior in aged animals. Physical activity also boosted BDNF and REST expression and decreased IL-1β and IL-10 expression in the hippocampus of aged animals. These results support the beneficial role of REST in the aged brain, which can be further enhanced by regular physical activity.
authors.affiliation: Laboratório de Bioenergética e Estresse Oxidativo (LABOX), Departamento de Bioquímica, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil; Grupo de Pesquisa Neurobiologia do Exercício Físico, Departamento de Fisioterapia, Universidade Federal de Santa Catarina, 88906-072 Araranguá, Brazil.
authors.forename: Karine Mathilde Campestrini
authors.initials: KMC
authors.lastname: Dallagnol
authors.affiliation: Laboratório de Bioenergética e Estresse Oxidativo (LABOX), Departamento de Bioquímica, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil; Grupo de Pesquisa Neurobiologia do Exercício Físico, Departamento de Fisioterapia, Universidade Federal de Santa Catarina, 88906-072 Araranguá, Brazil.
authors.forename: Aline Pertile
authors.initials: AP
authors.lastname: Remor
authors.affiliation: Laboratório de Bioenergética e Estresse Oxidativo (LABOX), Departamento de Bioquímica, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil.
authors.forename: Rodrigo Augusto
authors.initials: RA
authors.lastname: da Silva
authors.affiliation: Laboratório Experimental de Doenças Neurodegenerativas (LEXDON), Departamento de Farmacologia, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil.
authors.forename: Rui Daniel
authors.initials: RD
authors.lastname: Prediger
authors.affiliation: Laboratório de Bioenergética e Estresse Oxidativo (LABOX), Departamento de Bioquímica, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil; Grupo de Pesquisa Neurobiologia do Exercício Físico, Departamento de Fisioterapia, Universidade Federal de Santa Catarina, 88906-072 Araranguá, Brazil.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Latini
authors.affiliation: Laboratório de Bioenergética e Estresse Oxidativo (LABOX), Departamento de Bioquímica, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil; Grupo de Pesquisa Neurobiologia do Exercício Físico, Departamento de Fisioterapia, Universidade Federal de Santa Catarina, 88906-072 Araranguá, Brazil. Electronic address: aderbal.aguiar@ufsc.br.
authors.forename: Aderbal Silva
authors.initials: AS
authors.lastname: Aguiar
mesh_terms: D000339:Affect / Q000502:physiology; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D003863:Depression / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D007249:Inflammation / Q000378:metabolism*; D016753:Interleukin-10 / Q000097:blood; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D010805:Physical Conditioning, Animal / Q000502:physiology*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D053583:Interleukin-1beta; D016753:Interleukin-10
keywords: Aging; BDNF; Exercise; Interleukin-1 beta; Interleukin-10; Mood; Neuroinflammation; Physical activity; RE1-silencing transcription factor; REST