title: Crystal structure of the CupB6 adhesive tip from the chaperone-usher family of pili from Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa is a Gram-negative opportunistic bacterial pathogen that can cause chronic infection of the lungs of cystic fibrosis patients. Chaperone-usher systems in P. aeruginosa are known to translocate and assemble adhesive pili on the bacterial surface and contribute to biofilm formation within the host. Here, we report the crystal structure of the tip adhesion subunit CupB6 from the cupB1-6 gene cluster. The tip domain is connected to the pilus via the N-terminal donor strand from the main pilus subunit CupB1. Although the CupB6 adhesion domain bears structural features similar to other CU adhesins it displays an unusual polyproline helix adjacent to a prominent surface pocket, which are likely the site for receptor recognition.
authors.affiliation: Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom.
authors.forename: Masooma
authors.initials: M
authors.lastname: Rasheed
authors.affiliation: Queen Mary University of London, Department of Chemistry and Biochemistry, School of Biological and Chemical Sciences, Joseph Priestley Building, Mile End Road, London E1 4NS, United Kingdom.
authors.forename: James
authors.initials: J
authors.lastname: Garnett
authors.affiliation: Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Pérez-Dorado
authors.affiliation: Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom.
authors.forename: Daniela
authors.initials: D
authors.lastname: Muhl
authors.affiliation: Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom.
authors.forename: Alain
authors.initials: A
authors.lastname: Filloux
authors.affiliation: Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom. Electronic address: s.j.matthews@imperial.ac.uk.
authors.forename: Steve
authors.initials: S
authors.lastname: Matthews
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D036781:Fimbriae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D000072417:Protein Domains; D017433:Protein Structure, Secondary; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D018832:Molecular Chaperones; D011994:Recombinant Proteins; D036781:Fimbriae Proteins
keywords: Adhesin; Chaperone-usher; CupB6; From Pseudomonas aeruginosa