title: Development of an Immunochromatography Assay (QuickNavi-Ebola) to Detect Multiple Species of Ebolaviruses.
abstract: The latest outbreak of Ebola virus disease (EVD) in West Africa has highlighted the urgent need for the development of rapid and reliable diagnostic assays. We used monoclonal antibodies specific to the ebolavirus nucleoprotein to develop an immunochromatography (IC) assay (QuickNavi-Ebola) for rapid diagnosis of EVD. The IC assay was first evaluated with tissue culture supernatants of infected Vero E6 cells and found to be capable of detecting 103-104focus-forming units/mL of ebolaviruses. Using serum samples from experimentally infected nonhuman primates, we confirmed that the assay could detect the viral antigen shortly after disease onset. It was also noted that multiple species of ebolaviruses could be detected by the IC assay. Owing to the simplicity of the assay procedure and absence of requirements for special equipment and training, QuickNavi-Ebola is expected to be a useful tool for rapid diagnosis of EVD.
authors.affiliation: Division of Global Epidemiology, Research Center for Zoonosis Control.
authors.forename: Reiko
authors.initials: R
authors.lastname: Yoshida
authors.affiliation: Denka Seiken, Niigata, Japan.
authors.forename: Shino
authors.initials: S
authors.lastname: Muramatsu
authors.affiliation: Denka Seiken, Niigata, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akita
authors.affiliation: Denka Seiken, Niigata, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Saito
authors.affiliation: Denka Seiken, Niigata, Japan.
authors.forename: Miwa
authors.initials: M
authors.lastname: Kuwahara
authors.affiliation: Denka Seiken, Niigata, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kato
authors.affiliation: School of Veterinary Medicine, University of Zambia, Lusaka.
authors.forename: Katendi
authors.initials: K
authors.lastname: Changula
authors.affiliation: Division of Global Epidemiology, Research Center for Zoonosis Control.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Miyamoto
authors.affiliation: Division of Global Epidemiology, Research Center for Zoonosis Control.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kajihara
authors.affiliation: Division of Global Epidemiology, Research Center for Zoonosis Control.
authors.forename: Rashid
authors.initials: R
authors.lastname: Manzoor
authors.affiliation: Division of Global Epidemiology, Research Center for Zoonosis Control.
authors.forename: Wakako
authors.initials: W
authors.lastname: Furuyama
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana.
authors.forename: Andrea
authors.initials: A
authors.lastname: Marzi
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana.
authors.forename: Heinz
authors.initials: H
authors.lastname: Feldmann
authors.affiliation: School of Veterinary Medicine, University of Zambia, Lusaka.
authors.forename: Aaron
authors.initials: A
authors.lastname: Mweene
authors.affiliation: Institut National de Recherche Biomédicale Faculté de Médecine Vétérinaire, Université Pédagogique Nationale, Kinshasa, Democratic Republic of the Congo.
authors.forename: Justin
authors.initials: J
authors.lastname: Masumu
authors.affiliation: Institut National de Recherche Biomédicale.
authors.forename: Jimmy
authors.initials: J
authors.lastname: Kapeteshi
authors.affiliation: Institut National de Recherche Biomédicale.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Muyembe-Tamfum
authors.affiliation: Division of Global Epidemiology, Research Center for Zoonosis Control Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo School of Veterinary Medicine, University of Zambia, Lusaka.
authors.forename: Ayato
authors.initials: A
authors.lastname: Takada
mesh_terms: D000354:Africa, Western / Q000453:epidemiology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology*; D002846:Chromatography, Affinity / Q000379:methods*; D004196:Disease Outbreaks; D029043:Ebolavirus / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D006801:Humans; D009698:Nucleoproteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D009698:Nucleoproteins
keywords: EVD; Ebola virus; diagnosis; ebolavirus; filovirus; immunochromatography assay; monoclonal antibody; nucleoprotein