title: Glutathione-S-transferases and Chemotherapy Resistance of Hodgkin's Lymphoma Cell Lines.
abstract: BACKGROUND
  Glutathione-S-transferases (GSTs) are associated with multidrug resistance of tumor cells and are involved in drug detoxification and control of apoptosis. We analyzed the impact of GSTs on apoptosis of Hodgkin's lymphoma (HL) cells.
  
  
  METHODS
  Expression of GST isoforms in HL cell lines was assessed by analysis of DNA microarray data and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The impact of the GST inhibitor ethacrynic acid (EA) on HL cell survival was analyzed in vitro.
  
  
  RESULTS
  DNA microarray analysis and qRT-PCR analysis demonstrated higher expression of GST isoforms in chemoresistant HL cells. Therefore, GSTs may contribute to chemoresistance of HL cells. Incubation of GST-expressing chemoresistant L-1236 HL cells with EA significantly enhanced the activity of cisplatin against these cells.
  
  
  CONCLUSIONS
  Our data suggest that the combined treatment with chemotherapy and GST inhibitors such as EA might be an interesting option for patients with chemoresistant HL.
authors.affiliation: Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Toralf
authors.initials: T
authors.lastname: Bernig
authors.affiliation: Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Ritz
authors.affiliation: Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Grit
authors.initials: G
authors.lastname: Brodt
authors.affiliation: Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Ines
authors.initials: I
authors.lastname: Volkmer
authors.affiliation: Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany martin.staege@medizin.uni-halle.de.
authors.forename: Martin Sebastian
authors.initials: MS
authors.lastname: Staege
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000627:therapeutic use; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D004976:Ethacrynic Acid / Q000008:administration & dosage; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D006689:Hodgkin Disease / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D005982:Glutathione Transferase; D005978:Glutathione; D004976:Ethacrynic Acid; D002945:Cisplatin
keywords: Hodgkin's lymphoma; chemotherapy; ethacrynic acid; glutathione-S-transferases