title: Blue and Red Light-Evoked Pupil Responses in Photophobic Subjects with TBI.
abstract: Photophobia is a common symptom in individuals suffering from traumatic brain injury (TBI). Recent evidence has implicated blue light-sensitive intrinsically photosensitive retinal ganglion cells (ipRGCs) in contributing to the neural circuitry mediating photophobia in migraine sufferers. The goal of this work is to test the hypothesis that ipRGC function is altered in TBI patients with photophobia by assessing pupillary responses to blue and red light.
  
  
  
  Twenty-four case participants (mean age 43.3; 58% female), with mild TBI and self-reported photophobia, and 12 control participants (mean age 42.6; 58% female) were in this study. After 10 minutes of dark adaptation, blue (470 nm, 1 × 10 phots/s/cm) and red (625 nm, 7 × 10 phots/s/cm) flashing (0.1 Hz) light stimuli were delivered for 30 seconds to the dilated left eye while the right pupil was recorded. The amplitude of normalized pupil fluctuation (constriction and dilation) was quantified using Fourier fast transforms.
  
  
  
  In both case and control participants, the amplitude of pupil fluctuation was significantly less for the blue light stimuli as compared to the red light stimuli, consistent with a contribution of ipRGCs to these pupil responses. There was no significant difference in the mean pupil fluctuation amplitudes between the two participant groups, but case participants displayed greater variability in their pupil responses to the blue stimulus.
  
  
  
  Case and control participants showed robust ipRGC-mediated components in their pupil responses to blue light. The results did not support the hypothesis that ipRGCs are "hypersensitive" to light in TBI participants with photophobia. However, greater pupil response variability in the case subjects suggests that ipRGC function may be more heterogeneous in this group.
authors.affiliation: *OD, MS †OD, PhD ‡OD, MS, FAAO §OD, PhD, FAAO The Ohio State University College of Optometry, Columbus, Ohio (all authors).
authors.forename: Phillip T
authors.initials: PT
authors.lastname: Yuhas
authors.forename: Patrick D
authors.initials: PD
authors.lastname: Shorter
authors.forename: Catherine E
authors.initials: CE
authors.lastname: McDaniel
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Earley
authors.forename: Andrew T E
authors.initials: AT
authors.lastname: Hartwick
mesh_terms: D000328:Adult; D000070642:Brain Injuries, Traumatic / Q000503:physiopathology*; D005260:Female; D006801:Humans; D008027:Light; D008297:Male; D010775:Photic Stimulation; D020795:Photophobia / Q000503:physiopathology*; D011680:Pupil / Q000528:radiation effects*; D012025:Reflex, Pupillary / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000502:physiology