title: An Efficient Particle-Based DNA Circuit System: Catalytic Disassembly of DNA/PEG-Modified Gold Nanoparticle-Magnetic Bead Composites for Colorimetric Detection of miRNA.
abstract: An efficient particle-based DNA circuit system for a new colorimetric miRNA assay is designed and devised based on a catalytic disassembly strategy through a target miRNA-triggered DNA circuit mechanism. The new particle-based DNA circuit system shows a rapid color change as well as significant improvement of sensitivity without need for other enzymes or instruments.
authors.affiliation: Division of Materials Science, Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8573, Japan. oishi@ims.tsukuba.ac.jp.
authors.forename: Motoi
authors.initials: M
authors.lastname: Oishi
authors.affiliation: Division of Materials Science, Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8573, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Sugiyama
mesh_terms: D002384:Catalysis; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D060328:Magnetic Phenomena; D053768:Metal Nanoparticles / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis*; D008863:Microspheres; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D035683:MicroRNAs; D011092:Polyethylene Glycols; D006046:Gold; D004247:DNA
keywords: DNA circuit; gold nanoparticles; magnetic beads; miRNA; toehold-mediated DNA displacement reaction