title: Potential application of aerobic denitrifying bacterium Pseudomonas aeruginosa PCN-2 in nitrogen oxides (NOx) removal from flue gas.
abstract: Conventional biological removal of nitrogen oxides (NOx) from flue gas has been severely restricted by the presence of oxygen. This paper presents an efficient alternative for NOx removal at varying oxygen levels using the newly isolated bacterial strain Pseudomonas aeruginosa PCN-2 which was capable of aerobic and anoxic denitrification. Interestingly, nitric oxide (NO), as the obligatory intermediate, was negligibly accumulated during nitrate and nitrite reduction. Moreover, normal nitrate reduction with decreasing NO accumulation was realized under O2 concentration ranging from 0 to 100%. Reverse transcription and real-time quantitative polymerase chain reaction (RT-qPCR) analysis revealed that high efficient NO removal was attributed to the coordinate regulation of gene expressions including napA (for periplasmic nitrate reductase), nirS (for cytochrome cd1 nitrite reductase) and cnorB (for NO reductase). Further batch experiments demonstrated the immobilized strain PCN-2 possessed high capability of removing NO and nitrogen dioxide (NO2) at O2 concentration of 0-10%. A biotrickling filter established with present strain achieved high NOx removal efficiencies of 91.94-96.74% at inlet NO concentration of 100-500ppm and O2 concentration of 0-10%, which implied promising potential applications in purifying NOx contaminated flue gas.
authors.affiliation: MOE Key Laboratory of Regional Energy Systems Optimization, Resources and Environmental Research Academy, North China Electric Power University, Beijing 102206, China.
authors.forename: Maosheng
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors.forename: Can
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors.forename: Shufeng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors.forename: Mengyao
authors.initials: M
authors.lastname: Gui
authors.affiliation: Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China. Electronic address: nijinren@iee.pku.edu.cn.
authors.forename: Jinren
authors.initials: J
authors.lastname: Ni
mesh_terms: D000332:Aerobiosis; D005740:Gases; D005786:Gene Expression Regulation; D007220:Industrial Waste; D050901:Nitrate Reductase; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D009589:Nitrogen Oxides / Q000378:metabolism*; D010088:Oxidoreductases; D033903:Periplasmic Proteins; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*
chemical_list: D005740:Gases; D007220:Industrial Waste; D009566:Nitrates; D009573:Nitrites; D009589:Nitrogen Oxides; D033903:Periplasmic Proteins; D010088:Oxidoreductases; C034747:nitric-oxide reductase; D050901:Nitrate Reductase; C077913:periplasmic protein disulfide oxidoreductase
keywords: Aerobic denitrification; Biotrickling filter; Flue gas; NO(x) removal; Pseudomonas aeruginosa