title: Quantitative patterns of Hsps in tubular adenoma compared with normal and tumor tissues reveal the value of Hsp10 and Hsp60 in early diagnosis of large bowel cancer.
abstract: Large bowel carcinogenesis involves accumulation of genetic alterations leading to transformation of normal mucosa into dysplasia and, lastly, adenocarcinoma. It is pertinent to elucidate the molecular changes occurring in the pre-neoplastic lesions to facilitate early diagnosis and treatment. Heat shock proteins (Hsps), many of which are molecular chaperones, are implicated in carcinogenesis, and their variations with tumor progression encourage their study as biomarkers. There are many reports on Hsps and cancer but none to our knowledge on their systematic quantification in pre-neoplastic lesions of the large bowel. We performed immunohistochemical determinations of Hsp10, Hsp60, Hsp70, and Hsp90 in biopsies of large bowel tubular adenomas with moderate grade of dysplasia and compared to normal mucosa and adenocarcinoma with a moderate grade of differentiation (G2). A significant elevation of Hsp10 and Hsp60 only, i.e., in the absence of elevation of Hsp70 or Hsp90, in both epithelium and lamina propria was found in tubular adenoma by comparison with normal mucosa. In contrast, adenocarcinoma was characterized by the highest levels of Hsp10 and Hsp60 in epithelium and lamina propria, accompanied by the highest levels of Hsp70 only in epithelium and of Hsp90 only in lamina propria, by comparison with normal and tubular adenoma counterparts. Hsp10 and Hsp60 are promising biomarkers for early diagnosis of tubular adenoma and for its differentiation from more advanced malignant lesions. Hsp10 and Hsp60 may be implicated in carcinogenesis from its very early steps and, thus, are potentially convenient targets for therapy.
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Human Anatomy, University of Palermo, Via del Vespro 129, 90127, Palermo, Italy. francyrappa@hotmail.com.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy. francyrappa@hotmail.com.
authors.forename: Francesca
authors.initials: F
authors.lastname: Rappa
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Human Anatomy, University of Palermo, Via del Vespro 129, 90127, Palermo, Italy.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pitruzzella
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Human Anatomy, University of Palermo, Via del Vespro 129, 90127, Palermo, Italy.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Marino Gammazza
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Human Anatomy, University of Palermo, Via del Vespro 129, 90127, Palermo, Italy.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Barone
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Human Anatomy, University of Palermo, Via del Vespro 129, 90127, Palermo, Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Mocciaro
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Human Anatomy, University of Palermo, Via del Vespro 129, 90127, Palermo, Italy.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Tomasello
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Human Anatomy, University of Palermo, Via del Vespro 129, 90127, Palermo, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Carini
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Human Anatomy, University of Palermo, Via del Vespro 129, 90127, Palermo, Italy.
authors.forename: Felicia
authors.initials: F
authors.lastname: Farina
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Human Anatomy, University of Palermo, Via del Vespro 129, 90127, Palermo, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Zummo
authors.affiliation: Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.|Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore, Baltimore, MD, USA.|IMET, Columbus Center, Baltimore, MD, USA.
authors.forename: Everly
authors.initials: E
authors.lastname: Conway de Macario
authors.affiliation: Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.|Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore, Baltimore, MD, USA.|IMET, Columbus Center, Baltimore, MD, USA.
authors.forename: Alberto Jl
authors.initials: AJ
authors.lastname: Macario
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Human Anatomy, University of Palermo, Via del Vespro 129, 90127, Palermo, Italy.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cappello
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000378:metabolism*; D000236:Adenoma / Q000175:diagnosis / Q000378:metabolism*; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D016022:Case-Control Studies; D018835:Chaperonin 10 / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism*; D003106:Colon / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D018835:Chaperonin 10; D018834:Chaperonin 60; C060293:HSPD1 protein, human; D024101:Mitochondrial Proteins
keywords: Biomarker; Chaperone; Dysplasia; Hsps; Large bowel; Tubular adenoma