title: Cosynthesis of l-homophenylalanine and 2-phenylethanol by recombinant Saccharomyces cerevisiae expressing aspartate aminotransferase from Escherichia coli BL21(DE3).
abstract: The Escherichia coli aspartate aminotransferase gene was introduced into a high 2-phenylethanol (2-PEA) producing Saccharomyces cerevisiae YS58, and the recombinant strain of S. cerevisiae was utilized for the co-production of 2-PEA and l-homophenylalanine (L-HPA) via a fermentation process. The L-HPA productivity of the recombinant S. cerevisiae improved 78.9% in comparison to the wild-type S. cerevisiae. High yields of 43.7 mM L-HPA and 32.4 mM 2-PEA were achieved. As a result, the coupling of the biosynthesis process for these two products in the recombinant strain led to a more complete and efficient utilization of the substrate, l-phenylalanine.
authors.affiliation: College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Luo
authors.affiliation: College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China.
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Lin
authors.affiliation: College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China.
authors.forename: Suying
authors.initials: S
authors.lastname: Lin
authors.affiliation: College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Meng
authors.affiliation: College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China. Electronic address: wanghang@fzu.edu.cn.
authors.forename: Hang
authors.initials: H
authors.lastname: Wang
mesh_terms: D000613:Aminobutyrates / Q000378:metabolism*; D001219:Aspartate Aminotransferases / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005285:Fermentation; D015870:Gene Expression; D010626:Phenylethyl Alcohol / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000613:Aminobutyrates; D004274:DNA, Recombinant; C014328:2-amino-4-phenylbutyric acid; D001219:Aspartate Aminotransferases; D010626:Phenylethyl Alcohol
keywords: 2-Phenylethanol; Aspartate aminotransferase; Cosynthesis; Saccharomyces cerevisiae; l-Homophenylalanine