title: A novel carbon material with nanopores prepared using a metal-organic framework as precursor for highly selective enrichment of N-linked glycans.
abstract: Protein glycosylation plays a key role in many biological processes. In this study, a novel carbon material with nanopores was prepared by carbonization of metal-organic framework (MOF) Mil-101(Cr). The parent MOF assembled from metal ions with bridging organic linkers had many fascinating properties, such as ultrahigh surface area, suitable nanopore structure, and especially a large amount of carbon after being calcined. Due to the strong interactions between carbon and glycans as well as the size-exclusion effect of pore against protein, the N-linked glycans from standard glycoprotein or complex human serum proteins could be identified with high efficiency. The simple synthesis method as well as good enrichment efficiency made this novel carbon material a promising tool for glycosylation research.
authors.affiliation: Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, China.
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, China. mxgao@fudan.edu.cn.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, China.
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D000073396:Metal-Organic Frameworks / Q000737:chemistry*; D058608:Nanopores; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D000073396:Metal-Organic Frameworks; D011134:Polysaccharides; D002244:Carbon
keywords: Carbon material; Enrichment; MOF; N-linked glycans; Nanopores; Protein glycosylation