title: Ameliorative effect of vitamin C against hepatotoxicity induced by emamectin benzoate in rats.
abstract: In the present study, we aimed to assess the potential protective effect of ascorbic acid (AA) against emamectin benzoate (EMB)-induced hepatotoxicity. For this purpose, biochemical, histopathological and analytical investigations were performed. Male Wistar rats were distributed into three groups, that is, a control group, an EMB group given 10 mg EMB/kg body weight (BW) by gavage and an EMB + AA group given 10 mg EMB/kg BW and vitamin C intraperitoneally (200 mg/kg). The duration of the treatment was 28 days and the duration of the study was 42 days. There was a statistically significant increase of all hepatic biomarkers, that is, aspartate aminotransferase, alanine aminotransferase and gamma-glutamyltransferase activities, and glycemia, in EMB-treated group when compared with the control group. Light microscopic observations revealed variable signs of hepatotoxicity in the EMB group, which were represented by alteration of normal hepatic architecture, inflammatory cell infiltration, hepatocellular steatosis and foci of necrosis at 28 and 42 days post-treatment. However, co-treatment with vitamin C reduced EMB-related liver toxicity and diminished the abnormal biochemical and architectural damage. Emamectin B1a and B1b residues were detectable in all plasma samples of treated rats at 14, 21 and 28 days of treatment. The drug liver tissue concentration was significantly lower in EMB + AA group compared with EMB group at 28 and 42 days. In conclusion, the findings of the present study clearly indicate a significant protective action of vitamin C against EMB hepatotoxicity.
authors.affiliation: 1 Département de Biologie et Physiologie cellulaire, faculté des Sciences de la Nature et de la Vie, Université Blida 1, BP 270, route Soumaa, Blida, Algeria.|2 Laboratoire de Recherche d'Éco-Biologie Animale, École Normale Supérieure de Kouba Bachir El Ibrahimi, Algiers, Algeria.
authors.forename: H
authors.initials: H
authors.lastname: Khaldoun Oularbi
authors.affiliation: 3 CHU Lille, Unité Fonctionnelle de Toxicologie, F-59000 Lille, France.
authors.forename: C
authors.initials: C
authors.lastname: Richeval
authors.affiliation: 2 Laboratoire de Recherche d'Éco-Biologie Animale, École Normale Supérieure de Kouba Bachir El Ibrahimi, Algiers, Algeria.
authors.forename: N
authors.initials: N
authors.lastname: Lebaili
authors.affiliation: 4 Laboratoire des Ressorces Naturelles, Universite Mouloud Mammeri, Tizi-Ouzou, Algeria.
authors.forename: N
authors.initials: N
authors.lastname: Zerrouki-Daoudi
authors.affiliation: 2 Laboratoire de Recherche d'Éco-Biologie Animale, École Normale Supérieure de Kouba Bachir El Ibrahimi, Algiers, Algeria.
authors.forename: M
authors.initials: M
authors.lastname: Baha
authors.affiliation: 5 Laboratoire D'anatomie Pathologie CHRU Parnet, Alger, Algeria.
authors.forename: N
authors.initials: N
authors.lastname: Djennas
authors.affiliation: 2 Laboratoire de Recherche d'Éco-Biologie Animale, École Normale Supérieure de Kouba Bachir El Ibrahimi, Algiers, Algeria.|6 Univ. Lille, EA 4483 - IMPECS - IMPact de l'Environnement Chimique sur la Santé humaine, F-59000 Lille, France.
authors.forename: D
authors.initials: D
authors.lastname: Allorge
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000627:therapeutic use*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000473:pathology; D007306:Insecticides / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D020011:Protective Agents / Q000627:therapeutic use*; D017208:Rats, Wistar
chemical_list: D007306:Insecticides; D020011:Protective Agents; D007559:Ivermectin; C108024:emamectin benzoate; D001205:Ascorbic Acid
keywords: Vitamin C; emamectin benzoate; hepatotoxicity; plasma and liver residues