title: Reversibly monitoring oxidation and reduction events in living biological systems: Recent development of redox-responsive reversible NIR biosensors and their applications in in vitro/in vivo fluorescence imaging.
abstract: Reactive oxygen species (ROS) and changes in their redox cycles have great therapeutic potential for treating serious redox-related human diseases such as acute and chronic inflammation, diabetes, cancer and neurodegenerative disorders. This article presents a survey of the recently (2011-2016) developed NIR small-molecule biosensors for reversibly monitoring oxidation and reduction events in living cells and small animals through in vitro/in vivo fluorescence imaging. Emission and absorption profile, design strategy and fluorescence sensing mechanism, ROS selectivity and sensitivity, reversibility, ability of subcellular location and cytotoxicity are discussed for the NIR small-molecule biosensors capable of quantitatively, continuously and reversibly detecting transient ROS burst and redox changes at cellular level.
authors.affiliation: Institute for Computational Sciences and Engineering, Laboratory of New Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory and College of Physics, Qingdao University, Qingdao, 266071 China; State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023 People's Republic of China. Electronic address: tschu@dicp.ac.cn.
authors.forename: Tian-Shu
authors.initials: TS
authors.lastname: Chu
authors.affiliation: Laboratory of Pathogenic Biology, Medical College, Qingdao University, Qingdao, 266071 China.
authors.forename: Rui
authors.initials: R
authors.lastname: Lü
authors.affiliation: College of Chemical Science and Engineering, Qingdao University, Qingdao, 266071 China; State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023 People's Republic of China.
authors.forename: Bai-Tong
authors.initials: BT
authors.lastname: Liu
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002468:Cell Physiological Phenomena; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000295:instrumentation*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation*
chemical_list: D017382:Reactive Oxygen Species
keywords: In vitro/in vivo fluorescence imaging; Living cells and small animals; Reactive oxygen species; Redox changes; Redox-responsive reversible NIR biosensor