title: A novel nitrogen-dependent gene associates with the lesion mimic trait in wheat.
abstract: Using bulk segregant analysis (BSA) coupling with RNA-seq and DNA markers identified a potentially novel nitrogen-dependent lesion mimic gene Ndhrl1 on 2BS in wheat. Lesion mimic (LM) refers to hypersensitive reaction-like (HRL) traits that appear on leaf tissue in the absence of plant pathogens. In a wheat line P7001, LM showed up on the leaves under the 0 g nitrogen (N) treatment, but disappeared when sufficient N was supplied, suggesting that LM is N-responsive and N dosage dependent. Using BSA strategy together with RNA-seq and DNA markers, we identified an N-dependent LM gene (Ndhrl1) and mapped it to the short arm of chromosome 2B using an F5recombinant inbred population developed from the cross of P7001 × P216. The putative gene was delimited into an interval of 8.1 cM flanked by the CAPS/dCAPS markers 7hrC9 and 7hr2dc14, and co-segregated with the dCAPS marker 7hrdc2. This gene is most likely a novel gene for LM in wheat based on its chromosomal location. Further analysis of RNA-seq data showed that plant-pathogen interaction, nitrogen metabolism, zeatin biosynthesis and plant hormone signal transduction pathways were significantly differentially expressed between LM and non-LM lines.
authors.affiliation: Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops; Key Laboratory of Plant Functional Genomics of Ministry of Education; Wheat Research Center, Yangzhou University, Yangzhou, 225009, Jiangsu, China.|College of Plant Protection, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops; Key Laboratory of Plant Functional Genomics of Ministry of Education; Wheat Research Center, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Shi
authors.affiliation: Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops; Key Laboratory of Plant Functional Genomics of Ministry of Education; Wheat Research Center, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zheng
authors.affiliation: Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops; Key Laboratory of Plant Functional Genomics of Ministry of Education; Wheat Research Center, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
authors.forename: Changcheng
authors.initials: C
authors.lastname: Li
authors.affiliation: Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops; Key Laboratory of Plant Functional Genomics of Ministry of Education; Wheat Research Center, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225000, China.|Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA.
authors.forename: Guihua
authors.initials: G
authors.lastname: Bai
authors.affiliation: USDA-ARS Hard Winter Wheat Genetics Research Unit, Manhattan, KS, 66506, USA.
authors.forename: Derong
authors.initials: D
authors.lastname: Gao
authors.affiliation: College of Plant Protection, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Jincai
authors.initials: J
authors.lastname: Wu
authors.affiliation: Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops; Key Laboratory of Plant Functional Genomics of Ministry of Education; Wheat Research Center, Yangzhou University, Yangzhou, 225009, Jiangsu, China. taoli@yzu.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
mesh_terms: D002874:Chromosome Mapping; D005799:Genes, Dominant; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D009584:Nitrogen / Q000502:physiology*; D010641:Phenotype; D018749:RNA, Plant / Q000235:genetics; D017423:Sequence Analysis, RNA; D014908:Triticum / Q000235:genetics* / Q000502:physiology
chemical_list: D005819:Genetic Markers; D018749:RNA, Plant; D009584:Nitrogen