title: An Enzyme-based 1:2 Demultiplexer Interfaced with an Electrochemical Actuator.
abstract: An enzyme-based 1:2 demultiplexer is designed in a flow system composed of three cells where each one is modified with a different enzyme: hexokinase, glucose dehydrogenase and glucose-6-phosphate dehydrogenase. The Input signal activating the biocatalytic cascade is represented by glucose, while the Address signal represented by ATP is responsible for directing the Input signal to one of the output channels, depending on the logic value of the Address. The biomolecular 1:2 demultiplexer is extended to include two electrochemical actuators releasing entrapped DNA molecules in the active output channel. The modular design of the system allows for easy exchange and extension of the functional elements. The present demultiplexer can be easily integrated in various biomolecular logic systems, including different logic gates based on the enzyme- or DNA-based reactions, as well as containing different chemical actuators, for example, with a biomolecular release function.
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, 13699, USA.
authors.forename: Brian E
authors.identifier: http://orcid.org/0000-0003-3661-0639
authors.initials: BE
authors.lastname: Fratto
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, 13699, USA.
authors.forename: Nataliia
authors.identifier: http://orcid.org/0000-0003-1265-4864
authors.initials: N
authors.lastname: Guz
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, 13699, USA.
authors.forename: Tyler T
authors.initials: TT
authors.lastname: Fallon
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, 13699, USA.
authors.forename: Evgeny
authors.identifier: http://orcid.org/0000-0002-1618-4620
authors.initials: E
authors.lastname: Katz
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D055664:Electrochemical Techniques; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D006593:Hexokinase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000255:Adenosine Triphosphate; D004247:DNA; D042843:Glucose 1-Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase
keywords: biocomputing; demultiplexers; enzymes; flow cells; logic gates