title: A novel rodent Chapparvovirus in feces of wild rats.
abstract: Chapparvovirus, a recently determined new genus in the family Parvoviridae, can infect many species of animals including bats, chickens, and pigs. Here, using viral metagenomics method, we identified a novel Chapparvovirus from feces of wild rats and designated it as rat parvovirus 2 (RPV2). The nearly complete genome of RPV2 is 4222-nt long and includes two ORFs encoding a 654-aa nonstructural protein 1 (NS1) and a 472-aa capsid protein (VP), respectively. Phylogenetic analysis over the amino acid sequence of the NS1 showed that RPV2 clustered with Eidolon helvum parvovirus 2 (EHPV2), porcine parvovirus 7 (PPV7), and turkey parvovirus 1 (TP1), forming a separate clade. Sequence analysis indicated that the NS1 protein of RPV2 shared the highest amino acid sequence identity (51 %) with that of EHPV2. According to the genetic distance-based criteria, RPV2 identified here belongs to a novel species of Chapparvovirus.
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of China.
authors.forename: Shixing
authors.initials: S
authors.lastname: Yang
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of China.
authors.forename: Wang
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of China.
authors.forename: Xingli
authors.initials: X
authors.lastname: Fu
authors.affiliation: School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Gansu, 750000, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Shen
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of China. z0216wen@yahoo.com.|School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu, 200240, People's Republic of China. z0216wen@yahoo.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000821:virology; D002645:Chickens; D002681:China; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D016366:Open Reading Frames; D010321:Parvoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D051381:Rats; D012376:Rodent Diseases / Q000821:virology*; D013552:Swine; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Chapparvovirus; Genome structure; Viral Metagenomics; Wild rat