title: Wheat bran components modulate intestinal bacteria and gene expression of barrier function relevant proteins in a piglet model.
abstract: The objective of this study was to determine the impact of wheat bran and its main polysaccharides on intestinal bacteria and gene expression of intestinal barrier function relevant proteins. Thirty freshly weaned male piglets were assigned randomly to five dietary treatment groups with six piglets per group. Accordingly, five synthetic diets including a basal control diet without fiber components (CON), wheat bran diet (10% wheat bran, WB), arabinoxylan diet (AX), cellulose diet (CEL) and combined diet of arabinoxylan and cellulose (CB) were studied. The piglets were fed ad libitum for 30 d. Lower Escherichia coli (E. coli) populations in WB group and higher probiotic (Lactobacillus and Bifidobacterium) populations in groups fed diets containing arabinoxylan (WB, AX and CB) were observed and compared with CON group. Compared with CON group, the gene expressions of cystic fibrosis transmembrane conductance regulator (CFTR), calcium-activated chloride channel regulator 1 (CLCA1) and voltage-gated chloride channel 2 (CIC2) were suppressed in the WB group. And wheat bran down-regulated gene expression of pro-inflammation (TNF-α, IL-1β, IL-6) and TLRs/MyD88/NF-κB pathway compared with CON group. In conclusion, wheat bran and its main polysaccharides could change intestinal microflora and down-regulate the gene expression of intestinal barrier function relevant proteins in the distal small intestinal mucosa.
authors.affiliation: a College of Food Science, Sichuan Agricultural University , Ya'an , Sichuan , PR China.|b Department of Animal Production, Laboratory for Animal Nutrition and Animal Product Quality (LANUPRO) , Ghent University , Melle , Belgium.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: c Institute of Animal Nutrition, Sichuan Agricultural University , Chengdu , Sichuan , PR China.
authors.forename: Daiwen
authors.initials: D
authors.lastname: Chen
authors.affiliation: a College of Food Science, Sichuan Agricultural University , Ya'an , Sichuan , PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Qin
authors.affiliation: a College of Food Science, Sichuan Agricultural University , Ya'an , Sichuan , PR China.
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: c Institute of Animal Nutrition, Sichuan Agricultural University , Chengdu , Sichuan , PR China.
authors.forename: Lianqiang
authors.initials: L
authors.lastname: Che
authors.affiliation: c Institute of Animal Nutrition, Sichuan Agricultural University , Chengdu , Sichuan , PR China.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Huang
authors.affiliation: c Institute of Animal Nutrition, Sichuan Agricultural University , Chengdu , Sichuan , PR China.
authors.forename: Yuheng
authors.initials: Y
authors.lastname: Luo
authors.affiliation: a College of Food Science, Sichuan Agricultural University , Ya'an , Sichuan , PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: a College of Food Science, Sichuan Agricultural University , Ya'an , Sichuan , PR China.
authors.forename: Derong
authors.initials: D
authors.lastname: Lin
authors.affiliation: a College of Food Science, Sichuan Agricultural University , Ya'an , Sichuan , PR China.
authors.forename: Yaowen
authors.initials: Y
authors.lastname: Liu
authors.affiliation: a College of Food Science, Sichuan Agricultural University , Ya'an , Sichuan , PR China.
authors.forename: Guoquan
authors.initials: G
authors.lastname: Han
authors.affiliation: b Department of Animal Production, Laboratory for Animal Nutrition and Animal Product Quality (LANUPRO) , Ghent University , Melle , Belgium.
authors.forename: Stefaan
authors.initials: S
authors.lastname: DeSmet
authors.affiliation: b Department of Animal Production, Laboratory for Animal Nutrition and Animal Product Quality (LANUPRO) , Ghent University , Melle , Belgium.|d Department of Applied Biosciences , Ghent University , Gent , Belgium.
authors.forename: Joris
authors.initials: J
authors.lastname: Michiels
mesh_terms: D000818:Animals; D002482:Cellulose / Q000627:therapeutic use; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D004043:Dietary Fiber / Q000627:therapeutic use*; D004195:Disease Models, Animal; D064806:Dysbiosis / Q000378:metabolism / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D000069196:Gastrointestinal Microbiome; D018507:Gene Expression Regulation, Developmental; D007082:Ileum / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000378:metabolism; D007413:Intestinal Mucosa / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D008297:Male; D056692:Prebiotics; D021122:Protein Subunits / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D034421:Sus scrofa; D014908:Triticum / Q000737:chemistry*; D014886:Weaning; D014990:Xylans / Q000627:therapeutic use
chemical_list: D018118:Chloride Channels; D004043:Dietary Fiber; D018836:Inflammation Mediators; D056692:Prebiotics; D021122:Protein Subunits; D014990:Xylans; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D002482:Cellulose; C085118:arabinoxylan
keywords: Arabinoxylan; cellulose; chloride channel; gene expression