title: Functionalized photonic crystal for the sensing of Sarin agents.
abstract: The indiscriminate use of nerve agents by terrorist groups has attracted attention of the scientific communities toward the development of novel sensor technique for these deadly chemicals. A photonic crystal (PhC) hydrogel immobilized with butyrylcholinesterase (BuChE) was firstly prepared for the sensing of Sarin agents. Periodic polystyrene colloidal (240nm) array was embedded inside an acrylamide hydrogel, and then BuChE was immobilized inside the hydrogel matrix via condensation with 3-(diethoxyphosphoryloxy)-1,2,3-benzotriazin-4(3h)-one (DEPBT). It indicated that a total of 3.7 units of BuChE were immobilized onto the PhC hydrogel. The functionalized hydrogel recognized the Sarin agent and then shrunk, thus the diffraction of PhC hydrogel blue shifted significantly, and a limit of detection (LOD) of 10(-15)molL(-1) was achieved.
authors.affiliation: Institute of Chemical Defence, Beijing 102205, PR China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Yan
authors.affiliation: School of Chemical Engineering and Environment, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Fenglian
authors.initials: F
authors.lastname: Qi
authors.affiliation: Institute of Chemical Defence, Beijing 102205, PR China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Chemical Defence, Beijing 102205, PR China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Xu
authors.affiliation: School of Chemical Engineering and Environment, Beijing Institute of Technology, Beijing 100081, PR China; Institute of Chemical Defence, Beijing 102205, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: School of Chemical Engineering and Environment, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: m_zihui@yahoo.com.
authors.forename: Zihui
authors.initials: Z
authors.lastname: Meng
authors.affiliation: School of Chemical Engineering and Environment, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: liliqiu@bit.edu.cn.
authors.forename: Lili
authors.initials: L
authors.lastname: Qiu
authors.affiliation: School of Chemical Engineering and Environment, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: minxue@bit.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Xue
authors.affiliation: School of Chemical Engineering and Environment, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: School of Chemical Engineering and Environment, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Zequn
authors.initials: Z
authors.lastname: Yan
mesh_terms: D002091:Butyrylcholinesterase / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D057230:Limit of Detection; D010755:Organophosphates / Q000737:chemistry; D017785:Photons; D012524:Sarin / Q000032:analysis*; D014227:Triazines / Q000737:chemistry
chemical_list: C410327:3-(diethoxyphosphoryloxy)-1,2,3-benzotriazin-4(3H)-one; D010755:Organophosphates; D014227:Triazines; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012524:Sarin; D002091:Butyrylcholinesterase
keywords: Biosensor; Butyrylcholinesterase; Nerve agents; Photonic crystal; Sarin agent