title: Optical isopropanol biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH).
abstract: Isopropanol (IPA) is an important solvent used in industrial activity often found in hospitals as antiseptic alcohol rub. Also, IPA may have the potential to be a biomarker of diabetic ketoacidosis. In this study, an optical biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH) for IPA measurement was constructed and evaluated. An ultraviolet light emitting diode (UV-LED, λ=340nm) was employed as the excitation light to excite nicotinamide adenine dinucleotide (NADH). A photomultiplier tube (PMT) was connected to a two-way branch optical fiber for measuring the fluorescence emitted from the NADH. S-ADH was immobilized on the membrane to catalyze IPA to acetone and reduce NAD(+) to be NADH. This IPA biosensor shows highly sensitivity and selectivity, the calibration range is from 500 nmol L(-1) to 1mmolL(-1). The optimization of buffer pH, temperature, and the enzyme-immobilized method were also evaluated. The detection of IPA in nail related cosmetic using our IPA biosensor was also carried out. The results showed that large amounts of IPA were used in these kinds of cosmetics. This IPA biosensor comes with the advantages of rapid reaction, good reproducibility, and wide dynamic range, and is also expected to use for clinical IPA detections in serum or other medical and health related applications.
authors.affiliation: Graduate school of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Po-Jen
authors.initials: PJ
authors.lastname: Chien
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
authors.affiliation: Graduate school of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Takuma
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Toma
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Arakawa
authors.affiliation: Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-Cho, Suita-Shi, Osaka 564-0836, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Iwasaki
authors.affiliation: Graduate school of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. Electronic address: m.bdi@tmd.ac.jp.
authors.forename: Kohji
authors.initials: K
authors.lastname: Mitsubayashi
mesh_terms: D019840:2-Propanol / Q000737:chemistry*; D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003358:Cosmetics / Q000737:chemistry; D009243:NAD / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D003358:Cosmetics; D009243:NAD; D000429:Alcohol Oxidoreductases; C025192:isopropanol dehydrogenase (NADP); D019840:2-Propanol
keywords: Biosensor; Fiber-optic; Isopropanol; NADH; Secondary alcohol dehydrogenase; UV-LED