title: Preparation of chitosan grafted graphite composite for sensitive detection of dopamine in biological samples.
abstract: The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics. In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels. The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine. The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely. Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06μM. The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
authors.affiliation: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan.
authors.forename: Selvakumar
authors.initials: S
authors.lastname: Palanisamy
authors.affiliation: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan.
authors.forename: Kokulnathan
authors.initials: K
authors.lastname: Thangavelu
authors.affiliation: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan. Electronic address: smchen@ntut.edu.tw.
authors.forename: Shen-Ming
authors.initials: SM
authors.lastname: Chen
authors.affiliation: Department of Nanoscience and Nanotechnology, Karunya University, Coimbatore 64114, India.
authors.forename: P
authors.initials: P
authors.lastname: Gnanaprakasam
authors.affiliation: Division of Electrical and Electronic Engineering, School of Engineering, Manchester Metropolitan University, Manchester M1 5GD, United Kingdom.
authors.forename: Vijayalakshmi
authors.initials: V
authors.lastname: Velusamy
authors.affiliation: Key Laboratory of Education Ministry for Soft Chemistry and Functional Materials, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: xhliu@mail.njust.edu.cn.
authors.forename: Xiao-Heng
authors.initials: XH
authors.lastname: Liu
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D004298:Dopamine / Q000097:blood* / Q000652:urine*; D055664:Electrochemical Techniques; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans
chemical_list: D006108:Graphite; D048271:Chitosan; D004298:Dopamine
keywords: Biopolymer; Chitosan; Differential pulse voltammetry; Dopamine; Electro-oxidation; Graphite