title: Trophoblastic debris modifies endothelial cell transcriptome in vitro: a mechanism by which fetal cells might control maternal responses to pregnancy.
abstract: The mechanisms by which the fetus induces maternal physiological adaptations to pregnancy are unclear. Cellular debris, shed from the placental syncytiotrophoblast into the maternal blood and phagocytosed by maternal endothelial and immune cells, may be one of these mechanisms. Here we show that trophoblastic debris from normal first trimester placentae induces changes in the transcriptome and proteome of endothelial cells in vitro, which might contribute to the adaptation of the maternal cardiovascular system to pregnancy. Trophoblastic debris also induced endothelial cells to transcribe placenta-specific genes, including the vasodilator hormone CSH1, thereby expanding the effective functional size of the placenta. Our data suggest that the deportation of trophoblastic debris is an important part of the complex network of feto-maternal communication.
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Auckland, New Zealand.
authors.forename: J
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Auckland, New Zealand.
authors.forename: S Y
authors.initials: SY
authors.lastname: Lau
authors.affiliation: Department of Surgery, The University of Auckland, New Zealand.|Department of Molecular Medicine and Pathology, The University of Auckland, New Zealand.
authors.forename: C
authors.initials: C
authors.lastname: Blenkiron
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Auckland, New Zealand.
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Auckland, New Zealand.
authors.forename: J L
authors.initials: JL
authors.lastname: James
authors.affiliation: Department of Biochemistry, University of Otago, New Zealand.
authors.forename: T
authors.initials: T
authors.lastname: Kleffmann
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Auckland, New Zealand.
authors.forename: M
authors.initials: M
authors.lastname: Wise
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Auckland, New Zealand.
authors.forename: P R
authors.initials: PR
authors.lastname: Stone
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Auckland, New Zealand.
authors.forename: L W
authors.initials: LW
authors.lastname: Chamley
mesh_terms: D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D020543:Proteome / Q000032:analysis; D014327:Trophoblasts / Q000502:physiology*
chemical_list: D020543:Proteome