title: Anti-obesity effect of Crinum asiaticum var. japonicum Baker extract in high-fat diet-induced and monogenic obese mice.
abstract: This study determined the anti-obesity effect of Crinum asiaticum var. japonicum Baker extract (CAE) on adipocytes and obese mice. The inhibitory effects of CAE on adipocyte differentiation and adipogenesis were determined using differentiation induction medium in 3T3-L1 cells. To get an insight into underlying molecular actions of CAE, we investigated the changes in the expression levels of genes involved in lipogenesis by CAE treatment using qRT-PCR. CAE strongly suppressed adipocyte differentiation through downregulation of PPARγ, C/EBPα, C/EBP β, and aP2. CAE treatment could also suppress the expression levels of ACC, FAS, LPL and HMGCR gene in 3T3-L1 cells. Male C57BL/6 strain and C57BL/6J-ob/ob strain mice were fed with HFD containing 60% fat and normal diet in the presence or absence of 25, 50, and 100mg/kg CAE for 7 weeks. CAE supplementation could highly suppress the body weight gain and epididymal fat accumulation without changes in food uptake in both obese models. Increases in total cholesterol, LDL-cholesterol and triglyceride were highly suppressed in the presence of CAE. In summary, CAE has an anti-obesity effect and this anti-obesity potential might be associated with downregulation of genes involved in adipocyte differentiation and lipogenesis.
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Yong Joon
authors.initials: YJ
authors.lastname: Jeong
authors.affiliation: Department of Herbal Medicine Resource, Kangwon National University, Samcheok 25949, Republic of Korea.
authors.forename: Eun-Hwa
authors.initials: EH
authors.lastname: Sohn
authors.affiliation: Jeju Bio-Science Park, Jeju Technopark, Jeju 63241, Republic of Korea.
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Jung
authors.affiliation: Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 63608, Republic of Korea.
authors.forename: Weon-Jong
authors.initials: WJ
authors.lastname: Yoon
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Cho
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Inhye
authors.initials: I
authors.lastname: Kim
authors.affiliation: College of Medicine, Cardiovascular and Metabolic Disease Center and Department of Integrated Biomedical Science, Inje University, Busan 48108, Republic of Korea. Electronic address: lsr1113@inje.ac.kr.
authors.forename: Sung Ryul
authors.initials: SR
authors.lastname: Lee
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University, Yongin 17104, Republic of Korea. Electronic address: sckang@khu.ac.kr.
authors.forename: Se Chan
authors.initials: SC
authors.lastname: Kang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D031407:Crinum / Q000737:chemistry*; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D005247:Feeding Behavior / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D066292:Lipid Droplets / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D047495:PPAR gamma / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
chemical_list: D008055:Lipids; D047495:PPAR gamma; D010936:Plant Extracts; D012333:RNA, Messenger; D014157:Transcription Factors; D048031:JNK Mitogen-Activated Protein Kinases
keywords: Adipocyte differentiation; Crinum asiaticum var. japonicum Baker; High fat diet; Lipogenesis; Monogenic obese mice; Obesity