title: Bonding performance of experimental bioactive/biomimetic self-etch adhesives doped with calcium-phosphate fillers and biomimetic analogs of phosphoproteins.
abstract: This study examined the bonding performance and dentin remineralization potential of an experimental adhesive containing calcium-phosphate (Ca/P) micro-fillers, and self-etching primers doped with phosphoprotein biomimetic analogs (polyacrylic acid-(PAA) and/or sodium trimetaphosphate-(TMP)).
  
  
  
  Experimental self-etching primers doped with biomimetic analogs (PAA and/or TMP), and an adhesive containing Ca(2+), PO4(-3)-releasing micro-fillers (Ca/P) were formulated. Sound human dentin specimens were bonded and cut into sticks after aging (24h or 6 months) under simulated pulpal pressure (20cm H2O), and tested for microtensile bond strength (μTBS). Results were analyzed using two-way ANOVA and Tukey's test (p<0.05). Interfacial silver nanoleakage was assessed using SEM. Remineralization of EDTA-demineralized dentin was assessed through FTIR and TEM ultrastructural analysis.
  
  
  
  Application of the Ca/P-doped adhesive with or without dentin pre-treatments with the primer containing both biomimetic analogs (PAA and TMP) promoted stable μTBS over 6 months. Conversely, μTBS of the control primer and filler-free adhesive significantly decreased after 6 months. Nanoleakage decreased within the resin-dentin interfaces created using the Ca/P-doped adhesives. EDTA-demineralized dentin specimens treated the Ca/P-doped adhesive and the primer containing PAA and TMP showed phosphate uptake (FTIR analysis), as well as deposition of needle-like crystallites at intrafibrillar level (TEM analysis).
  
  
  
  The use of Ca/P-doped self-etching adhesives applied in combination with analogs of phosphoproteins provides durable resin-dentin bonds. This approach may represent a suitable bonding strategy for remineralization of intrafibrillar dentin collagen within the resin-dentin interface.
authors.affiliation: Department of Restorative Dentistry, Dental Materials Division, School of Dentistry, Campinas State University (UNICAMP), Piracicaba, SP, Brazil.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Abuna
authors.affiliation: Department of Restorative Dentistry, Federal University of Ceara, Fortaleza, Brazil.
authors.forename: Victor P
authors.initials: VP
authors.lastname: Feitosa
authors.affiliation: Department of Restorative Dentistry, Dental Materials Division, School of Dentistry, Campinas State University (UNICAMP), Piracicaba, SP, Brazil.
authors.forename: Americo Bortolazzo
authors.initials: AB
authors.lastname: Correr
authors.affiliation: Polymer Chemistry & Biomaterials Group, University of Ghent, Ghent, Belgium.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cama
authors.affiliation: Department of Restorative Dentistry, Dental Materials Division, School of Dentistry, Campinas State University (UNICAMP), Piracicaba, SP, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Giannini
authors.affiliation: Department of Restorative Dentistry, Dental Materials Division, School of Dentistry, Campinas State University (UNICAMP), Piracicaba, SP, Brazil.
authors.forename: Mario A
authors.initials: MA
authors.lastname: Sinhoreti
authors.affiliation: Department of Oral Biology, The Dental College of Georgia, Augusta, GA, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.affiliation: Dental Biomaterials, Preventive and Minimally Invasive Dentistry, Departamento de Odontologia, Facultad de Ciencias de la Salud, Universidad CEU-Cardenal Herrera,, Valencia, Spain. Electronic address: salvatore.sauro@uch.ceu.es.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sauro
mesh_terms: D000269:Adhesives; D032701:Biomimetics; D002118:Calcium; D001840:Dental Bonding; D003738:Dental Cements; D003804:Dentin; D017220:Dentin-Bonding Agents; D006801:Humans; D008422:Materials Testing; D010750:Phosphoproteins; D019279:Resin Cements; D013718:Tensile Strength
chemical_list: D000269:Adhesives; D003738:Dental Cements; D017220:Dentin-Bonding Agents; D010750:Phosphoproteins; D019279:Resin Cements; D002118:Calcium
keywords: Adhesive; Biomimetic remineralization; Collagen; Dentin; Self-etching