title: Mutation in the S gene a determinant of the hepatitis B virus associated with concomitant HBsAg and anti-HBs in a population in Northeastern Brazil.
abstract: Mutations in the a determinant of S gene may develop co-existence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) in the serum of infected hepatitis B virus (HBV) individuals. Mutations in this region may change the antigenicity of HBsAg, which in turn, lead to escape of neutralizing action of anti-HBs antibodies. This study identified individuals with concomitant HBsAg and anti-HBs serological markers in individuals of Maranhão, Northeastern Brazil. Samples from a population-based study were evaluated for HBsAg, anti-HBs, and anti-HBc, and those that tested positive for simultaneous HBsAg and anti-HBs were submitted to HBV DNA quantification and S gene characterization by Sanger sequencing. Mutations were investigated in the a determinant located in major hydrophilic region (MHR) of the S gene. Among 3,984 samples analyzed, 92 (2.3%) were positive for HBsAg and three had the atypical HBsAg and anti-HBs-positive profile (3.26%). The frequency of HBsAg and anti-HBs co-existence was similar to previous studies. Only one individual harbored mutation in the S gene a determinant associated with this profile. Little is known about this phenomenon; however, studies as ours may contribute for future enlightenment of this important issue. J. Med. Virol. 89:458-462, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Graduate Program of Health Sciences, Federal University of Maranhão, Maranhão, Brazil.
authors.forename: Ingrid
authors.initials: I
authors.lastname: de Campos Albuquerque
authors.affiliation: Graduate Program of Health Sciences, Federal University of Maranhão, Maranhão, Brazil.
authors.forename: Marinilde Teles
authors.initials: MT
authors.lastname: Sousa
authors.affiliation: Department of Gastroenterology, University of Sao Paulo School of Medicine, São Paulo, Brazil.
authors.forename: Max Diego Cruz
authors.initials: MD
authors.lastname: Santos
authors.affiliation: Graduate Program of Health Sciences, Federal University of Maranhão, Maranhão, Brazil.
authors.forename: Jomar Diogo Costa
authors.initials: JD
authors.lastname: Nunes
authors.affiliation: Maranhão Clinical Research Center, University Hospital of the Federal University of Maranhão, Maranhão, Brazil.
authors.forename: Maria Josélia Diniz
authors.initials: MJ
authors.lastname: Moraes
authors.affiliation: Department of Gastroenterology, University of Sao Paulo School of Medicine, São Paulo, Brazil.
authors.forename: Michele Soares
authors.initials: MS
authors.lastname: Gomes-Gouvêa
authors.affiliation: Department of Gastroenterology, University of Sao Paulo School of Medicine, São Paulo, Brazil.
authors.forename: João Renato Rebelo
authors.initials: JR
authors.lastname: Pinho
authors.affiliation: Department of Gastroenterology, University of Sao Paulo School of Medicine, São Paulo, Brazil.
authors.forename: Flair José
authors.initials: FJ
authors.lastname: Carrilho
authors.affiliation: Department of Nursing, Federal University of Maranhão, Maranhão, Brazil.
authors.forename: Lena Maria Barros
authors.initials: LM
authors.lastname: Fonseca
authors.affiliation: Department of Medicine, Federal University of Maranhão, Maranhão, Brazil.
authors.forename: Adalgisa
authors.initials: A
authors.lastname: de Sousa Paiva Ferreira
mesh_terms: D000328:Adult; D001938:Brazil / Q000453:epidemiology; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000097:blood; D005260:Female; D006509:Hepatitis B / Q000175:diagnosis* / Q000453:epidemiology* / Q000821:virology; D006510:Hepatitis B Antibodies / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000097:blood* / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D017422:Sequence Analysis, DNA; D016036:Seroepidemiologic Studies; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens
keywords: HBsAg; antibody; hepatitis B; mutation