title: Molecular Scanning and Morpho-Physiological Dissection of Component Mechanism in Lens Species in Response to Aluminium Stress.
abstract: Aluminium (Al) stress was imposed on 285 lentil genotypes at seedling stage under hydroponics to study its effects on morpho-physiological traits where resistant cultigens and wilds showed minimum reduction in root and shoot length and maximum root re-growth (RRG) after staining. Molecular assortment based on 46 simple sequence repeat (SSR) markers clustered the genotypes into 11 groups, where wilds were separated from the cultigens. Genetic diversity and polymorphism information content (PIC) varied between 0.148-0.775 and 0.140-0.739, respectively. Breeding lines which were found to be most resistant (L-7903, L-4602); sensitive cultivars (BM-4, L-4147) and wilds ILWL-185 (resistant), ILWL-436 (sensitive) were grouped into different clusters. These genotypes were also separated on the basis of population structure and Jaccard's similarity index and analysed to study Al resistance mechanism through determination of different attributes like localization of Al and callose, lipid peroxidation, secretion of organic acids and production of antioxidant enzymes. In contrast to sensitive genotypes, in resistant ones most of the Al was localized in the epidermal cells, where its movement to apoplastic region was restricted due to release of citrate and malate. Under acidic field conditions, resistant genotypes produced maximum seed yield/plant as compared to sensitive genotypes at two different locations i.e. Imphal, Manipur, India and Basar, Arunanchal Pradesh, India during 2012-13, 2013-14 and 2014-15. These findings suggest that Al stress adaptation in lentil is through exclusion mechanism and hybridization between the contrasting genotypes from distinct clusters can help in development of resistant varieties.
authors.affiliation: Division of Genetics, Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Singh
authors.affiliation: Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Madan
authors.initials: M
authors.lastname: Pal
authors.affiliation: Division of Genetics, Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Chandan Kumar
authors.initials: CK
authors.lastname: Singh
authors.affiliation: Division of Genetics, Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Taunk
authors.affiliation: Division of Genetics, Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Jain
authors.affiliation: Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Ashish Kumar
authors.initials: AK
authors.lastname: Chaturvedi
authors.affiliation: Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Sadhana
authors.initials: S
authors.lastname: Maurya
authors.affiliation: Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Sourabh
authors.initials: S
authors.lastname: Karwa
authors.affiliation: Division of Soil Science and Agricultural Chemistry, Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Rajendra
authors.initials: R
authors.lastname: Singh
authors.affiliation: National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Ram Sewak Singh
authors.initials: RS
authors.lastname: Tomar
authors.affiliation: KVK West Siang, ICAR RC for NEH Region, A.P. Centre, Basar, Arunachal Pradesh, India.
authors.forename: Rita
authors.initials: R
authors.lastname: Nongthombam
authors.affiliation: KVK Imphal East, Andro, Central Agricultural University, Imphal, Manipur, India.
authors.forename: Nandini
authors.initials: N
authors.lastname: Chongtham
authors.affiliation: Directorate of Extension Education, Central Agricultural University, Imphal, Manipur, India.
authors.forename: Moirangthem Premjit
authors.initials: MP
authors.lastname: Singh
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D016000:Cluster Analysis; D006861:Hydrogen Peroxide / Q000737:chemistry; D027806:Lens Plant / Q000187:drug effects* / Q000502:physiology; D015227:Lipid Peroxidation; D013312:Stress, Physiological
chemical_list: D006861:Hydrogen Peroxide; D000535:Aluminum