title: Biosynthesis of xanthan gum by Xanthomonas campestris LRELP-1 using kitchen waste as the sole substrate.
abstract: Herein, we report the production of xanthan gum by fermentation using kitchen waste as the sole substrate. The kitchen waste was firstly pretreated by a simple hydrolysis method, after which the obtained kitchen waste hydrolysate was diluted with an optimal ratio 1:2. In a 5-L fermentor, the maximum xanthan production, reducing sugar conversion and utilization rates reached 11.73g/L, 67.07% and 94.82%, respectively. The kinetics of batch fermentation was also investigated. FT-IR and XRD characterizations confirmed the fermentation product as xanthan gum. TGA analyses showed that the thermal stability of the xanthan gum obtained in this study was similar to commercial sample. The molecular weights of xanthan gum were measured to be 0.69-1.37×10(6)g/mol. The maximum pyruvate and acetyl contents in xanthan gum were 6.11% and 2.49%, respectively. This study provides a cost-effective solution for the reusing of kitchen waste and a possible low-cost approach for xanthan production.
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, China. Electronic address: lipanyu920124@sina.com.
authors.forename: Panyu
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, China. Electronic address: 869312527@qq.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, China. Electronic address: 407369800@qq.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, China. Electronic address: 424329309@qq.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, China. Electronic address: 215927760@qq.com.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, China. Electronic address: ybwang@scu.edu.cn.
authors.forename: Yabo
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, China. Electronic address: xietonghui@scu.edu.cn.
authors.forename: Tonghui
authors.initials: T
authors.lastname: Xie
authors.affiliation: Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, China. Electronic address: zhangyongkui@scu.edu.cn.
authors.forename: Yongkui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001709:Biotechnology; D005285:Fermentation; D006868:Hydrolysis; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D013696:Temperature; D014866:Waste Products; D016959:Xanthomonas campestris / Q000378:metabolism*
chemical_list: D011135:Polysaccharides, Bacterial; D014866:Waste Products; C002563:xanthan gum
keywords: 2,4-Dinitrophenylhydrazone (PubChem CID: 9659690); 5-Hydroxymethylfurfural (PubChem CID: 237332); Acetyl substituent; Furfural (PubChem CID: 7362); Kinetic analysis; Kitchen waste; Pyruvate (PubChem CID: 107735); Pyruvate substituent; Waste reuse; Xanthan gum; Xanthan gum (PubChem CID: 7107)