title: Comparative Proteomic Analysis of Extracellular Vesicles Isolated by Acoustic Trapping or Differential Centrifugation.
abstract: Extracellular vesicles (ECVs), including microparticles and exosomes, are submicrometer membrane vesicles released by diverse cell types upon activation or stress. Circulating ECVs are potential reservoirs of disease biomarkers, and the complexity of these vesicles is significantly lower compared to their source, blood plasma, which makes ECV-based biomarker studies more promising. Proteomic profiling of ECVs is important not only to discover new diagnostic or prognostic markers but also to understand their roles in biological function. In the current study, we investigated the protein composition of plasma-derived ECVs isolated by acoustic seed trapping. Additionally, the protein composition of ECVs isolated with acoustic trapping was compared to that isolated with a conventional differential centrifugation protocol. Finally, the proteome of ECVs originating from ST-elevation myocardial infarction patients was compared with that of healthy controls using label-free LC-MS quantification. The acoustic trapping platform allows rapid and automated preparation of ECVs from small sample volumes, which are therefore well-suited for biobank repositories. We found that the protein composition of trapped ECVs is very similar to that isolated by the conventional differential centrifugation method.
authors.forename: Melinda
authors.initials: M
authors.lastname: Rezeli
authors.forename: Olof
authors.initials: O
authors.lastname: Gidlöf
authors.forename: Mikael
authors.initials: M
authors.lastname: Evander
authors.forename: Paulina
authors.initials: P
authors.lastname: Bryl-Górecka
authors.forename: Ramasri
authors.initials: R
authors.lastname: Sathanoori
authors.forename: Patrik
authors.initials: P
authors.lastname: Gilje
authors.affiliation: Department of Experimental Design and Bioinformatics, Faculty of Agriculture and Biology, Warsaw University of Life Sciences , Warsaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Pawłowski
authors.affiliation: Analytical Biochemistry, Department of Pharmacy, University of Groningen , Groningen, The Netherlands.
authors.forename: Péter
authors.initials: P
authors.lastname: Horvatovich
authors.forename: David
authors.initials: D
authors.lastname: Erlinge
authors.forename: György
authors.initials: G
authors.lastname: Marko-Varga
authors.affiliation: Department of Biomedical Engineering, Dongguk University , Seoul, Korea.
authors.forename: Thomas
authors.initials: T
authors.lastname: Laurell
mesh_terms: D000162:Acoustics; D001798:Blood Proteins / Q000032:analysis*; D002498:Centrifugation; D000067128:Extracellular Vesicles / Q000737:chemistry*; D006801:Humans; D046210:Microfluidic Analytical Techniques; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000473:pathology; D040901:Proteomics
chemical_list: D001798:Blood Proteins