title: Hypolipidemic, antiobesity and cardioprotective effects of sardinelle meat flour and its hydrolysates in high-fat and fructose diet fed Wistar rats.
abstract: OBJECTIVE
  The present study aims to evaluate the antiobesity, hypolipidemic and cardioprotective effects of fermented sardinelle (Sardinella aurita) protein hydrolysates (FSPHs) produced with two proteolytic bacteria, Bacillus subtilis A26 (FSPH-A26) and Bacillus amyloliquefaciens An6 (FSPH-An6).
  
  
  METHODS
  Wistar rats were fed during 10weeks a standard laboratory diet, a high caloric diet (HCD) and a HCD coupled with the oral administration of sardinelle meat flour (SMF) or FSPHs.
  
  
  RESULTS
  HCD caused hyperlipidemia and increased body weight (BW). The daily oral administration of FSPHs or SMF reduced the total cholesterol (TC), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-c) serum levels, and increased the level of high-density lipoprotein cholesterol (HDL-c). Nevertheless, FSPHs were found to be more efficient than SMF. FSPHs also lowered hepatic TC and TG content and decreased the pancreatic lipase activity. Further, the administration of FSPHs or SMF decreased the BW gain, the food intake and the relative epididymal adipose tissue weight. FSPHs exhibited a potent cardioprotective effect against heart attack, which was demonstrated by returning atherogenic indexes to their normal levels and the conservation of standard histological structure of the heart and aorta.
  
  
  CONCLUSIONS
  The overall results indicate that FSPHs contained bioactive peptides which significantly attenuated hyperlipidemia, and might reduce the risk of cardiovascular disease (CVD) in rats fed HCD.
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P.O. Box 1173, Sfax 3038, Tunisia. Electronic address: inesjemil1987@gmail.com.
authors.forename: Ines
authors.initials: I
authors.lastname: Jemil
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P.O. Box 1173, Sfax 3038, Tunisia.
authors.forename: Ola
authors.initials: O
authors.lastname: Abdelhedi
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P.O. Box 1173, Sfax 3038, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Nasri
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenue Agustín Escardino 7, Paterna, 46980, Valencia, Spain.
authors.forename: Leticia
authors.initials: L
authors.lastname: Mora
authors.affiliation: Laboratory of Biochemistry, CHU Hedi Chaker, Sfax 3029, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Marrekchi
authors.affiliation: Laboratory of Biochemistry, CHU Hedi Chaker, Sfax 3029, Tunisia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Jamoussi
authors.affiliation: Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax (FSS), University of Sfax, P.O. Box 95, Sfax 3052, Tunisia.
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: ElFeki
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P.O. Box 1173, Sfax 3038, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hajji
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenue Agustín Escardino 7, Paterna, 46980, Valencia, Spain.
authors.forename: Fidel
authors.initials: F
authors.lastname: Toldrá
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P.O. Box 1173, Sfax 3038, Tunisia.
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000009:adverse effects* / Q000494:pharmacology; D005394:Fish Flour; D029941:Fish Proteins / Q000737:chemistry / Q000494:pharmacology; D005632:Fructose / Q000009:adverse effects* / Q000494:pharmacology; D000960:Hypolipidemic Agents / Q000737:chemistry / Q000494:pharmacology; D008055:Lipids / Q000097:blood; D008297:Male; D009765:Obesity / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy / Q000503:physiopathology; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004041:Dietary Fats; D029941:Fish Proteins; D000960:Hypolipidemic Agents; D008055:Lipids; D011492:Protein Hydrolysates; D005632:Fructose
keywords: Antiobesity; Cardioprotective; Fermented sardinelle protein hydrolysates; High caloric diet; Hypolipidemic