title: [The Study of Lipofibroblasts Differentiation of CD90⁺ and CD90⁻ Orbital Fibroblasts Subsets in Patients with TAO].
abstract: OBJECTIVE
  Divided orbital fibroblasts from patients with thyroid-associated ophthalmopathy (TAO) into CD90⁺ and CD90⁻ subsets respect to surface CD90 expression, then determined whether CD90⁺ and/or CD90⁻ fibroblasts were capable of differentiating into lipofibroblasts.
  
  
  METHODS
  Fibroblasts subset separation into CD90⁺ and CD90⁻ subsets was accomplished by three to four rounds of magnetic bead selection, then treated with 3-isobutyl-1-lmethylxanthine (IBMX), insulin and dexamethasone which was an known inducer of the lipofibroblastic phenotype, then the cells were observed every day to find Lipid droplets. Oil red O staining were conducted at 5 d, 10 d, 15 d, 20 d and 25 d after inducing. The percent of lipofibroblasts were calculated.
  
  
  RESULTS
  The ratio in fibroblast derived from extraocular muscles of differentiating into lipofibroblast is less than from connective/adipose tissue (P < 0.05). The ratio of CD90⁺ fibroblast is less than CD90⁻ fibroblast (P < 0.05). CD90⁺ cells derived from extraocular miscles could not be induced to differentiate into lipofibroblast. The ratio in CD90⁻ fibroblast from connective/adipose tissue is highest (P < 0.05).
  
  
  CONCLUSIONS
  Orbital fibroblast which has the function of differentiating into lipofibroblast is mainly CD90⁻ connective/adipose tissue.
authors.forename: Lu
authors.initials: L
authors.lastname: Gan
authors.forename: Qing-li
authors.initials: QL
authors.lastname: Luo
authors.forename: Fei
authors.initials: F
authors.lastname: Tang
authors.forename: Wen-wen
authors.initials: WW
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Tang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D049970:Graves Ophthalmopathy / Q000473:pathology*; D006801:Humans; D018482:Muscle, Skeletal / Q000166:cytology; D009915:Orbit / Q000166:cytology*; D018800:Thy-1 Antigens / Q000378:metabolism*
chemical_list: D018800:Thy-1 Antigens