title: Study of Binding Interaction between Pif80 Protein Fragment and Aragonite.
abstract: Pif is a crucial protein for the formation of the nacreous layer in Pinctada fucata. Three non-acidic peptide fragments of the aragonite-binding domain (Pif80) are selected, which contain multiple copies of the repeat sequence DDRK, to study the interaction between non-acidic peptides and aragonite. The polypeptides DDRKDDRKGGK (Pif80-11) and DDRKDDRKGGKDDRKDDRKGGK (Pif80-22) have similar binding affinity to aragonite. Solid-state NMR data indicate that the backbones of Pif80-11 and Pif80-22 peptides bound on aragonite adopt a random-coil conformation. Pif80-11 is a lot more effective than Pif80-22 in promoting the nucleation of aragonite on the substrate of β-chitin. Our results suggest that the structural arrangement at a protein-mineral interface depends on the surface structure of the mineral substrate and the protein sequence. The side chains of the basic residues, which function as anchors to the aragonite surface, have uniform structures. The role of basic residues as anchors in protein-mineral interaction may play an important role in biomineralization.
authors.affiliation: Department of Chemistry, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.
authors.forename: Yuan-Peng
authors.initials: YP
authors.lastname: Du
authors.affiliation: Department of Chemistry, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.
authors.forename: Hsun-Hui
authors.initials: HH
authors.lastname: Chang
authors.affiliation: Department of Chemistry, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.
authors.forename: Sheng-Yu
authors.initials: SY
authors.lastname: Yang
authors.affiliation: Instrumentation Center, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.
authors.forename: Shing-Jong
authors.initials: SJ
authors.lastname: Huang
authors.affiliation: Institute of Chemistry, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei 115, Taiwan.
authors.forename: Yu-Ju
authors.initials: YJ
authors.lastname: Tsai
authors.affiliation: Institute of Chemistry, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei 115, Taiwan.
authors.forename: Joseph Jen-Tse
authors.initials: JJ
authors.lastname: Huang
authors.affiliation: Department of Chemistry, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.
authors.forename: Jerry Chun Chung
authors.initials: JC
authors.lastname: Chan
mesh_terms: D000818:Animals; D002119:Calcium Carbonate / Q000737:chemistry / Q000378:metabolism*; D003460:Crystallization; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D049911:Pinctada / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D016326:Extracellular Matrix Proteins; D010446:Peptide Fragments; D002119:Calcium Carbonate