title: Ursolic acid regulates aging process through enhancing of metabolic sensor proteins level.
abstract: We previously reported that Ursolic Acid (UA) ameliorates skeletal muscle performance through satellite cells proliferation and cellular energy status. In studying the potential role of the hypothalamus in aging, we developed a strategy to pursue UA effects on the hypothalamus anti-aging proteins such as; SIRT1, SIRT6, PGC-1β and α-Klotho. In this study, we used a model of aging animals (C57BL/6). UA dissolved in Corn oil (20mg/ml) and then administrated (200mg/Kg i.p injection) to mice, twice daily for 7days. After treatment times, the mice perfused and the hypothalamus isolated for preparing of tissue to Immunofluorescence microscopy. The data illustrated that UA significantly increased SIRT1 (∼3.5±0.3 folds) and SIRT-6 (∼1.5±0.2 folds) proteins overexpression (P<0.001). In addition, our results showed that UA enhanced α-Klotho (∼3.3±0.3) and PGC-1β (∼2.6±0.2 folds) proteins levels (P<0. 01). In this study, data were analyzed using SPSS 16 (ANOVA test). To the best of our knowledge, it seems that UA through enhancing of anti-aging biomarkers (SIRT1 and SIRT6) and PGC-1β in hypothalamus regulates aging-process and attenuates mitochondrial-related diseases. In regard to the key role of α-Klotho in aging, our data indicate that UA may be on the horizon to forestall diseases of aging.
authors.affiliation: Department of Biochemistry, Faculty of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
authors.forename: Soroush Alaghehband
authors.initials: SA
authors.lastname: Bahrami
authors.affiliation: Department of Biochemistry, Faculty of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran. Electronic address: Nuredin.bakhtiary@modares.ac.ir.
authors.forename: Nuredin
authors.initials: N
authors.lastname: Bakhtiari
mesh_terms: D000375:Aging / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D000090265:Klotho Proteins; D008810:Mice, Inbred C57BL; D056564:Sirtuin 1 / Q000378:metabolism*; D037761:Sirtuins / Q000378:metabolism*; D014315:Triterpenes / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects; D000097245:Ursolic Acid
chemical_list: D002352:Carrier Proteins; D005966:Glucuronidase; D000090265:Klotho Proteins; D056564:Sirtuin 1; D037761:Sirtuins; D014315:Triterpenes; D000097245:Ursolic Acid; C501860:Sirt6 protein, mouse; C470545:Sirt1 protein, mouse
keywords: PGC-1β; SIRT1; SIRT6; Ursolic acid; α-Klotho