title: Selective adsorption of hemoglobin with polyoxometalate-derived hybrid by solidification of super-lacunary phosphotungstate polyoxoanions.
abstract: A novel polyoxometalate (POM)-based hybrid P8W48-APTS is prepared by the solidification of super-lacunary P8W48O184(40-) polyoxoanions with APTS in an acidic medium. The oxygen (O(-)) atoms in P8W48O184(40-) are bound to silicon atoms in APTS by the formation of Si-O linkage through dehydration condensation. The solidification is confirmed by characterizations with XRD, FT-IR, TGA, SEM and EDXS. Selective isolation of proteins of interest, hemoglobin (Hb) in this case, from complex sample matrices is achieved by using P8W48-APTS hybrid as adsorbent under controlled conditions. 5.0mg of P8W48-APTS hybrid results in an adsorption efficiency of 93% for 100mgL(-1) hemoglobin in 1.0mL sample solution at pH 7. The adsorption behavior of Hb onto P8W48-APTS hybrid fits Langmuir adsorption model, corresponding to an adsorption capacity of 355.0mgg(-1). The retained Hb could be readily recovered with either a SDS solution (0.1molL(-1)) or a Na3PO4 (0.1molL(-1)) solution as stripping reagent, providing recoveries of 94.6% or 83.9%, respectively. The biological activity of Hb remains 96.7% after an adsorption/desorption process (with elution by SDS), which illustrates virtually no change on the conformation of hemoglobin. The P8W48-APTS hybrid has been applied for the selective adsorption of Hb from human whole blood, and the results are demonstrated by SDS-PAGE assay.
authors.affiliation: Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Zhang
authors.affiliation: Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Hu
authors.affiliation: Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China. Electronic address: chenxuwei@mail.neu.edu.cn.
authors.forename: Xu-Wei
authors.initials: XW
authors.lastname: Chen
authors.affiliation: Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China. Electronic address: jianhuajrz@mail.neu.edu.cn.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D001774:Blood Chemical Analysis / Q000379:methods*; D006454:Hemoglobins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared; D017973:Tungsten Compounds / Q000737:chemistry / Q000378:metabolism*; D000099294:Polyoxometalates
chemical_list: D006454:Hemoglobins; D017973:Tungsten Compounds; C102296:polyoxometalate I; D000099294:Polyoxometalates
keywords: Hemoglobin; Selective adsorption; Super-lacunary phosphotungstate