title: Prevalence of H. pylori Infection and Atrophic Gastritis in a Population-based Screening with Serum Biomarker Panel (GastroPanel®) in St. Petersburg.
abstract: OBJECTIVE
  Russian Federation is among the high-incidence countries for gastric cancer (GC), with the incidence being projected to continue increasing. Using a non-invasive blood test with four stomach-specific biomarkers (pepsinogen-I (PG-I) and -II (PG-II), amidated gastrin-17 (G-17) and Helicobacter pylori (HP) IgG antibodies) in a hospital-based screening setting, we aimed to determine the prevalence of GC risk conditions: HP-infection and atrophic gastritis (AG).
  
  
  METHODS
  A population-derived cohort of 918 asymptomatic subjects (646 women and 272 men) with a mean age of 51.8 years (range=26-83) was examined with the GastroPanel® (GP) test. GP results were verified by gastroscopy and biopsies (the Updated Sydney System (USS) classification for all test-positive AG cases and for random 5% test-negatives (n=263) to correct for the verification bias.
  
  
  RESULTS
  Of the 918 subjects, only 199 (21.7%) tested completely normal, while 76.7% (704/918) had HP-infection. Altogether, in 99 subjects (10.8%), GP suggested AG: atrophic gastritis in the antrum (AGA) (n=21), atrophic gastritis in the corpus (AGC) (n=69) or atrophic pangastritis (AGpan) (n=9). The overall concordance between GP and USS classification was 82.5% (217/263) with weighted kappa intra-class correlation coefficient (ICC)=0.875 (95% confidence interval (CI)=0.840-0.901). The sensitivity/specificity balance in receiver operating characteristic (ROC) analysis for PG-I as a marker of moderate/severe AGC (AGC2+) had area under the curve (AUC)=0.895 (95%CI=0.837-0.953). Using the AGC2+ end-point, verification bias-corrected specificity of PGI reached 96.4% (95%CI=94.7-97.9) and that of PGI/PGII ratio 94.6% (95%CI=92.6-96.3), with inevitable erosion in sensitivities.
  
  
  CONCLUSIONS
  While capable of detecting the subjects at risk for GC (HP and/or AG), GP should be the cost-effective means to break the current ominous trend in GC incidence in Russian Federation.
authors.affiliation: Department of Endoscopy, the Leningrad Regional Oncological Dispensary, St. Petersburg, Russian Federation.
authors.forename: Laslo D
authors.initials: LD
authors.lastname: Roman
authors.affiliation: Department of Endoscopy, the Leningrad Regional Oncological Dispensary, St. Petersburg, Russian Federation.
authors.forename: Ruslan
authors.initials: R
authors.lastname: Lukyanchuk
authors.affiliation: The Federal State Institute of Public Health 'The Nikiforov Russian Center of Emergency and Radiation Medicine', St. Petersburg, Russian Federation.
authors.forename: Oleg A
authors.initials: OA
authors.lastname: Sablin
authors.affiliation: The Federal State Institute of Public Health 'The Nikiforov Russian Center of Emergency and Radiation Medicine', St. Petersburg, Russian Federation.
authors.forename: Elena I
authors.initials: EI
authors.lastname: Araslanova
authors.affiliation: Department of Research and Development, Biohit Oyj, Helsinki, Finland.
authors.forename: Carita
authors.initials: C
authors.lastname: Eklund
authors.affiliation: Department of Research and Development, Biohit Oyj, Helsinki, Finland.
authors.forename: Panu
authors.initials: P
authors.lastname: Hendolin
authors.affiliation: Department of Research and Development, Biohit Oyj, Helsinki, Finland.
authors.forename: Lea
authors.initials: L
authors.lastname: Paloheimo
authors.affiliation: Department of Clinical Research, Biohit Oyj, Helsinki, Finland Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, SP, Brazil kari.syrjanen@biohit.fi.
authors.forename: Kari
authors.initials: K
authors.lastname: Syrjänen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D005260:Female; D005757:Gastritis, Atrophic / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D016481:Helicobacter Infections / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008403:Mass Screening; D008875:Middle Aged; D015995:Prevalence; D012372:ROC Curve; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D014408:Biomarkers, Tumor
keywords: GastroPanel; Helicobacter pylori; antrum; atrophic gastritis; biomarker; corpus; gastric cancer; gastrin-17; pepsinogens; population-based; risk factors; screening; verification bias