title: Elevated expression of WWP2 in human lung adenocarcinoma and its effect on migration and invasion.
abstract: Lung cancer has been a hot area of research because of its high incidence and mortality. In this study, WWP2, an E3 ubiquitin ligase, is proposed to be an oncoprotein contributing to lung tumorigenesis. We attempted to determine if WWP2 gene expression is correlated with the development of human lung adenocarcinoma. Real-time PCR and western blotting were used to detect the expression of WWP2 in 65 paired lung adenocarcinoma and adjacent normal lung tissues. We found that WWP2 expression was elevated in lung adenocarcinoma tissues and was correlated with the tumor differentiation stage, TNM stage and presence of lymph node metastasis. We performed CCK-8 and colony formation assays and found that down-regulation of WWP2 inhibited proliferation in A549 and SPC-A-1 cells. A wound healing assay and trans-well invasion assays showed that down-regulation of WWP2 inhibited the migration and invasion of lung adenocarcinoma cells. It could be predicted from these data that elevated expression of WWP2 may play a role in facilitating the development of lung adenocarcinoma.
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Ultrasound, People's Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China. Electronic address: zgj@zzu.edu.cn.
authors.forename: Guojun
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014410:Tumor Stem Cell Assay; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C465283:WWP2 protein, human; D044767:Ubiquitin-Protein Ligases
keywords: Lung adenocarcinoma; Migration and invasion; Proliferation; WWP2