title: Cytotoxic Oxygenated Steroids from the Soft Coral Nephthea erecta.
abstract: A new 10-demethylated steroid, nephtheasteroid A (1), a new 19-oxygenated steroid, nephtheasteroid B (2) as well as five known steroids 3-7 were isolated from the organic extract of a Taiwanese soft coral Nephthea erecta. The structure was determined by means of IR, MS, and NMR techniques. Among these metabolites, 1 is rarely found in steroids possessing a 19-norergostane skeleton. In vitro cytotoxicity study using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay revealed that compounds 3 and 4 exhibited cytotoxicity against human chronic myelogenous leukemia (K562), human acute lymphoblastic leukemia (Molt-4), human T lymphoblastoid (Sup-T1), and human leukemic monocyte lymphoma (U937), with IC50of 6.5-14.0 µM.
authors.affiliation: Department of Food Science and Nutrition, Meiho University.
authors.forename: Tsung-Chang
authors.initials: TC
authors.lastname: Tsai
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Huang
authors.forename: Shih-Kai
authors.initials: SK
authors.lastname: Chou
authors.forename: Ming-Cheng
authors.initials: MC
authors.lastname: Shih
authors.forename: Ching-Ying
authors.initials: CY
authors.lastname: Chiang
authors.forename: Jui-Hsin
authors.initials: JH
authors.lastname: Su
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008968:Molecular Conformation; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013261:Sterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D013256:Steroids; D013261:Sterols