title: Lactobacillus Casei Decreases Organophosphorus Pesticide Diazinon Cytotoxicity in Human HUVEC Cell Line.
abstract: OBJECTIVE
  Exposure to diazinon can trigger acute and chronic toxicity and significantly induces DNA damage and proapoptotic effects in different human cells. Due to the significance of probiotic bacteria antitoxin effect, this study aimed to investigate the effect of Lactobacillus casei on diazinon (DZN) cytotoxicity in human umbilical vein endothelial cells (HUVEC) in vitro.
  
  
  METHODS
  The cytotoxicity assessments were performed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test, DAPI (4',6-diamidino-2-phenylindole) staining and flow cytometric methodologies.
  
  
  RESULTS
  Cytotoxic assessments through flow cytometry/ DAPI staining demonstrated that apoptosis is the main cytotoxic mechanism of diazinon in HUVEC cells and L. casei could decrease the diazinon cytotoxic effects on toxicants.
  
  
  CONCLUSIONS
  the screen of total bacterial secreted metabolites can be considered as a wealthy source to find the new active compounds to introduce as reducing agricultural remained pesticide cytotoxicity effects on the human food chain.
authors.affiliation: Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.; Dryland Agricultural Research Institute (DARI), Agricultural Research, Education and Extension Organization (AREEO), Maragheh, Iran.
authors.forename: Hasan
authors.initials: H
authors.lastname: Bagherpour Shamloo
authors.affiliation: Dryland Agricultural Research Institute (DARI), Agricultural Research, Education and Extension Organization (AREEO), Maragheh, Iran.
authors.forename: Saber
authors.initials: S
authors.lastname: Golkari
authors.affiliation: Tuberculosis & Lung Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.; Student Research Committee, Faculty of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Faghfoori
authors.affiliation: Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: AliAkbar
authors.initials: A
authors.lastname: Movassaghpour
authors.affiliation: Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
authors.forename: Hajie
authors.initials: H
authors.lastname: Lotfi
authors.affiliation: Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Barzegari
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.; Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Yari Khosroushahi
keywords: Apoptosis; Cytotoxicity; Diazinon; Lactobacillus casei; Probiotic