title: A Role of Suppressor of Cytokine Signaling 2 in the Regulation of Ecdysteroid Signaling Pathway in Procambarus clarkii.
abstract: A suppressor of cytokine signaling 2 (SOCS-2) homolog was identified from the red crayfish Procambarus clarkii. Phylogenic analysis showed that P. clarkii SOCS-2 (Pc-SOCS-2) was very similar to that of invertebrates. The sequence contains a central SH2 domain, a conserved SOCS box, and a variable N-terminal domain. Real-time polymerase chain reaction showed that Pc-SOCS-2 mRNA level was higher in muscle and heart than in the other tissues examined. A 40-kDa recombinant protein was successfully expressed and purified from Escherichia coli cells as confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Pc-SOCS-2 was significantly downregulated in the hepatopancreas after 20-hydroxyecdysone injection. Knockdown of Pc-SOCS-2 gene by small interfering RNA transfection in the hepatopancreas increased the expression levels of 20-hydroxyecdysone-responsive genes at 48 hr. Taken together, these results suggest that Pc-SOCS-2 is a negative regulator of ecdysteroid signaling transduction.
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, 230036, People's Republic of China. zhubaojian@ahau.edu.cn.
authors.forename: Baojian
authors.initials: B
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
authors.forename: Lishang
authors.initials: L
authors.lastname: Dai
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Yu
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
authors.forename: Daojun
authors.initials: D
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
authors.forename: Chaoliang
authors.initials: C
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003400:Astacoidea / Q000378:metabolism*; D004441:Ecdysterone / Q000494:pharmacology*; D015870:Gene Expression; D009928:Organ Specificity; D010802:Phylogeny; D016415:Sequence Alignment; D015398:Signal Transduction / Q000187:drug effects; D050826:Suppressor of Cytokine Signaling Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D060829:Arthropod Proteins; D050826:Suppressor of Cytokine Signaling Proteins; D004441:Ecdysterone