title: An extract from pork bones containing osteocalcin improves glucose metabolism in mice by oral administration.
abstract: Osteocalcin (OC) is a bone-derived hormone that regulates energy metabolism. OC exists in two forms, carboxylated (GlaOC) and uncaboxylated (GluOC), but only the latter appears to have an endocrine function. In this study, we prepared an extract containing both Gla- and GluOC from boiled pork bone using 0.2 M carbonate buffer at pH 9.5, and tested whether the extract had beneficial effects on improving metabolic parameters in obese mice. The extract equivalent of 1.2 μg of GluOC/mouse was orally administrated to C57BL/6 female mice fed a high-fat, high-sucrose diet. Daily oral administration of the extract for four weeks decreased blood glucose levels and promoted glucose tolerance as well as insulin sensitivity. Our study shows for the first time that boiled pork bones are a source material for osteocalcin in the large-scale production of supplements designed to improve glucose metabolism.
authors.affiliation: a Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science , Kyushu University , Fukuoka , Japan.|b OBT Research Center, Faculty of Dental Science , Kyushu University , Fukuoka , Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Mizokami
authors.affiliation: a Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science , Kyushu University , Fukuoka , Japan.
authors.forename: DaGuang
authors.initials: D
authors.lastname: Wang
authors.affiliation: c Faculty of Agriculture , Graduate School of Kyushu University , Fukuoka , Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: a Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science , Kyushu University , Fukuoka , Japan.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: d Division of Applied Pharmacology , Kyushu Dental University , Kitakyushu , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takeuchi
authors.affiliation: c Faculty of Agriculture , Graduate School of Kyushu University , Fukuoka , Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Matsui
authors.affiliation: a Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science , Kyushu University , Fukuoka , Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Hirata
keywords: adipocyte; glucose metabolism; oral administration; osteocalcin; pork bone