title: Antibacterial Activity of MTA Fillapex and AH 26 Root Canal Sealers at Different Time Intervals.
abstract: BACKGROUND
  The main goal of endodontic treatment is elimination of bacteria and their by-products from infected root canals. This study compared the antibacterial effect of two different sealers, AH 26 and MTA Fillapex, on 4 microorganisms 24, 48 and 72 h and 7 days after mixing.
  
  
  METHODS
  The microorganisms used in this study consisted of Lactobacillus acidophilus (ATCC 4356), Lactobacillus casei (ATCC 39392), Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 29212). This test is based on the growth of bacteria and turbidity measurement technique using a spectrophotometer, and direct contact was conducted. Multiple comparisons were carried out using repeated-measures ANOVA followed by Tukey's test and student's t-test. The level of significance was set at 0.05.
  
  
  RESULTS
  The antibacterial activity in the indirect technique was more than the technique with both sealers. In the direct technique the antibacterial activity on all microorganisms were lower for MTA Fillapex sealer. In the indirect technique, both sealers exhibited similar antibacterial properties.
  
  
  CONCLUSIONS
  The antibacterial effect of MTA Fillapex sealer was significantly less than that of AH 26 sealer in the direct technique. The antibacterial effects of both sealers were similar in the indirect technique.
authors.affiliation: Department of Endodontic, Dental School, Tabriz University of Medical Sciences, Tabriz, Iran;
authors.forename: Farnaz
authors.initials: F
authors.lastname: Jafari
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;
authors.forename: Hossein
authors.initials: H
authors.lastname: Samadi Kafil
authors.affiliation: Department of Orthodontic, Dental School, Ilam University of Medical Sciences, Ilam, Iran ;
authors.forename: Sanaz
authors.initials: S
authors.lastname: Jafari
authors.affiliation: Department of Microbiology, Medical School, Tabriz University of Medical Sciences, Tabriz, Iran ;
authors.forename: Mohammad
authors.initials: M
authors.lastname: Aghazadeh
authors.affiliation: Dentist, Dental School, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Momeni
keywords: Antibacterial; Enterococcus faecalis; Lactobacillus; MTA Fillapex; Sealers; Staphylococcus