title: Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida.
abstract: Living and non-living biomass of Pseudomonas putida A (ATCC 12633) was used as biosorbent for the removing of Al(3+) from aqueous solutions. The process was stable with time, efficient at pH 4.3 and between 15°C and 42°C. Two isotherms models were applied to describe the interaction between the biosorbent and Al(3+). Non-living biomass of P. putida A (ATCC 12633) was found to be the most efficient at adsorbing Al(3+) with a maximum sorption capacity of 0.55mg Al(3+)/gr adsorbent and with 36×10(5) binding sites of Al(3+)/microorganisms. Infrared spectroscopy analysis shows that the biosorbent present some vibrational band of functional groups that change in presence of Al(3+): hydroxyl, carboxyl and phosphate. Considering that Al(3+) binds to the phosphate group of phosphatidylcholine, non-viable biomass of P. putida PB01 (mutant lacking phosphatidylcholine) was used. Aluminum adsorption of the parental strain was 30 times higher than values registered in P. putida PB01 (36×10(5) sites/microorganism vs 1.2×10(5) sites/microorganism, respectively). This result evidenced that the absence of phosphatidylcholine significantly affected the availability of the binding sites and consequently the efficiency of the biomass to adsorb Al(3+).
authors.affiliation: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors.forename: Paola Sabrina
authors.initials: PS
authors.lastname: Boeris
authors.affiliation: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors.forename: María Del Rosario
authors.initials: Mdel R
authors.lastname: Agustín
authors.affiliation: Departamento de Química Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors.forename: Diego Fernando
authors.initials: DF
authors.lastname: Acevedo
authors.affiliation: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina. Electronic address: glucchesi@exa.unrc.edu.ar.
authors.forename: Gloria Inés
authors.initials: GI
authors.lastname: Lucchesi
mesh_terms: D000327:Adsorption; D000535:Aluminum / Q000032:analysis / Q000493:pharmacokinetics*; D001673:Biodegradation, Environmental; D018533:Biomass; D006863:Hydrogen-Ion Concentration; D016958:Pseudomonas putida / Q000737:chemistry / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000493:pharmacokinetics*
chemical_list: D014874:Water Pollutants, Chemical; D000535:Aluminum
keywords: Aluminum; Biosorption; Pseudomonas putida; Toxic metals