title: Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser.
abstract: Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.
authors.affiliation: Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.|Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Tomas
authors.initials: T
authors.lastname: Ekeberg
authors.affiliation: Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Svenda
authors.affiliation: Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors.forename: M Marvin
authors.initials: MM
authors.lastname: Seibert
authors.affiliation: Information Génomique et Structurale (UMR7256) CNRS &Aix-Marseille Université, Institut de Microbiologie de la Méditerranée (FR3479), Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France.
authors.forename: Chantal
authors.identifier: http://orcid.org/0000-0003-1875-4049
authors.initials: C
authors.lastname: Abergel
authors.affiliation: Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors.forename: Filipe R N C
authors.initials: FR
authors.lastname: Maia
authors.affiliation: Information Génomique et Structurale (UMR7256) CNRS &Aix-Marseille Université, Institut de Microbiologie de la Méditerranée (FR3479), Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Seltzer
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.|LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: DePonte
authors.affiliation: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.|European XFEL, Albert-Einstein-Ring 19, 22761 Hamburg, Germany.
authors.forename: Andrew
authors.initials: A
authors.lastname: Aquila
authors.affiliation: Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors.forename: Jakob
authors.initials: J
authors.lastname: Andreasson
authors.affiliation: Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.|Commissariat à l'énergie atomique et aux énergies alternatives, Centre d'études de Saclay, 91191 Gif sur Yvette cedex, France.
authors.forename: Bianca
authors.initials: B
authors.lastname: Iwan
authors.affiliation: Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.|Molekyl- och kondenserade materiens fysik, Institutionen för Fysik och Astronomi, Uppsala University, Lägerhyddsvägen 1 (Box 524), SE-751 20 Uppsala, Sweden.
authors.forename: Olof
authors.initials: O
authors.lastname: Jönsson
authors.affiliation: Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors.forename: Daniel
authors.initials: D
authors.lastname: Westphal
authors.affiliation: Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors.forename: Duško
authors.initials: D
authors.lastname: Odić
authors.affiliation: Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors.forename: Inger
authors.initials: I
authors.lastname: Andersson
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Anton
authors.initials: A
authors.lastname: Barty
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.|LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Liang
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.|The University of Melbourne, 161 Barry Street, Parkville, 3010 Victoria, Australia.
authors.forename: Andrew V
authors.initials: AV
authors.lastname: Martin
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Gumprecht
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Fleckenstein
authors.affiliation: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Saša
authors.initials: S
authors.lastname: Bajt
authors.affiliation: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Miriam
authors.initials: M
authors.lastname: Barthelmess
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Nicola
authors.initials: N
authors.lastname: Coppola
authors.affiliation: Information Génomique et Structurale (UMR7256) CNRS &Aix-Marseille Université, Institut de Microbiologie de la Méditerranée (FR3479), Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Claverie
authors.affiliation: Stanford PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.|Centre for BioImaging Sciences, National University of Singapore, 14 Science Drive 4 Blk S1 A, Singapore 117546, Singapore.
authors.forename: N Duane
authors.initials: ND
authors.lastname: Loh
authors.affiliation: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors.forename: Christoph
authors.initials: C
authors.lastname: Bostedt
authors.affiliation: Synchrotron SOLEIL, L'orme des Merisiers roundabout of St Aubin, 91190 Saint Aubin, France.
authors.forename: John D
authors.initials: JD
authors.lastname: Bozek
authors.affiliation: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors.forename: Jacek
authors.initials: J
authors.lastname: Krzywinski
authors.affiliation: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors.forename: Marc
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authors.affiliation: Stanford PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
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authors.initials: MJ
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authors.affiliation: Stanford PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors.forename: Christina Y
authors.initials: CY
authors.lastname: Hampton
authors.affiliation: Stanford PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors.forename: Raymond G
authors.initials: RG
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authors.affiliation: Lawrence Livermore National Laboratory, 7000 East Avenue, Mail Stop L-211, Livermore, California 94551, USA.
authors.forename: Matthias
authors.identifier: http://orcid.org/0000-0002-5244-0791
authors.initials: M
authors.lastname: Frank
authors.affiliation: Max-Planck-Institut für medizinische Forschung, Jahnstr. 29, 69120 Heidelberg, Germany.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Shoeman
authors.affiliation: Max-Planck-Institut für medizinische Forschung, Jahnstr. 29, 69120 Heidelberg, Germany.
authors.forename: Lukas
authors.initials: L
authors.lastname: Lomb
authors.affiliation: Max-Planck-Institut für medizinische Forschung, Jahnstr. 29, 69120 Heidelberg, Germany.|Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Lutz
authors.initials: L
authors.lastname: Foucar
authors.affiliation: Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.|Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany.
authors.forename: Sascha W
authors.initials: SW
authors.lastname: Epp
authors.affiliation: Max-Planck-Institut für medizinische Forschung, Jahnstr. 29, 69120 Heidelberg, Germany.|Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.|Department of Physics, J.R. Macdonald Laboratory, Kansas State University, 116 Cardwell Hall, Manhattan, Kansas 66506, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Rolles
authors.affiliation: Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.|Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany.|Department of Physics, J.R. Macdonald Laboratory, Kansas State University, 116 Cardwell Hall, Manhattan, Kansas 66506, USA.
authors.forename: Artem
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authors.affiliation: PNSensor GmbH, Otto-Hahn-Ring 6, 81739 Munich, Germany.
authors.forename: Robert
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authors.affiliation: PNSensor GmbH, Otto-Hahn-Ring 6, 81739 Munich, Germany.
authors.forename: Andreas
authors.initials: A
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authors.affiliation: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 Munich, Germany.|Max-Planck-Institut für extraterrestrische Physik, Giessenbachstrasse, 85741 Garching, Germany.
authors.forename: Nils
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authors.affiliation: PNSensor GmbH, Otto-Hahn-Ring 6, 81739 Munich, Germany.
authors.forename: Peter
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authors.affiliation: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 Munich, Germany.|Max-Planck-Institut für extraterrestrische Physik, Giessenbachstrasse, 85741 Garching, Germany.
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authors.affiliation: Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.|Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany.
authors.forename: Benedikt
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authors.affiliation: Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.|Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany.
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authors.affiliation: Max-Planck-Institut für medizinische Forschung, Jahnstr. 29, 69120 Heidelberg, Germany.
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authors.affiliation: Max-Planck-Institut für medizinische Forschung, Jahnstr. 29, 69120 Heidelberg, Germany.|Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Ilme
authors.initials: I
authors.lastname: Schlichting
authors.affiliation: PNSensor GmbH, Otto-Hahn-Ring 6, 81739 Munich, Germany.|Universität Siegen, Emmy-Noether Campus, Walter Flex Str. 3, 57068 Siegen, Germany.
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authors.affiliation: Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.|Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany.|Physikalisch-Technische Bundesanstalt, Bundesallee 100, 38116 Braunschweig, Germany.
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authors.affiliation: Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.|Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany.
authors.forename: Carlo
authors.initials: C
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authors.affiliation: Max-Planck-Institut für medizinische Forschung, Jahnstr. 29, 69120 Heidelberg, Germany.|Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Faton
authors.initials: F
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authors.affiliation: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 Munich, Germany.|Max-Planck-Institut für extraterrestrische Physik, Giessenbachstrasse, 85741 Garching, Germany.
authors.forename: Günter
authors.initials: G
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authors.affiliation: PNSensor GmbH, Otto-Hahn-Ring 6, 81739 Munich, Germany.
authors.forename: Christian
authors.initials: C
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authors.affiliation: PNSensor GmbH, Otto-Hahn-Ring 6, 81739 Munich, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Soltau
authors.affiliation: Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany.
authors.forename: Sebastian
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authors.affiliation: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
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authors.initials: H
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authors.affiliation: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
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authors.affiliation: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Heinz
authors.initials: H
authors.lastname: Graafsma
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.|University of Hamburg, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Henry
authors.identifier: http://orcid.org/0000-0002-4655-1743
authors.initials: H
authors.lastname: Chapman
authors.affiliation: Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.|European XFEL, Albert-Einstein-Ring 19, 22761 Hamburg, Germany.
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mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D003625:Data Collection; D004583:Electrons; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D007834:Lasers; D056686:Mimiviridae; D008962:Models, Theoretical; D010316:Particle Size; D012542:Scattering, Radiation; D014961:X-Ray Diffraction; D014965:X-Rays