title: Capturing Identity and Fate Ex Vivo: Stem Cells from the Mouse Blastocyst.
abstract: During mouse preimplantation development, three molecularly, morphologically, and spatially distinct lineages are formed, the embryonic epiblast, the extraembryonic primitive endoderm, and the trophectoderm. Stem cell lines representing each of these lineages have now been derived and can be indefinitely maintained and expanded in culture, providing an unlimited source of material to study the interplay of tissue-specific transcription factors and signaling pathways involved in these fundamental cell fate decisions. Here we outline our current understanding of the derivation, maintenance, and properties of these in vitro stem cell models representing the preimplantation embryonic lineages.
authors.affiliation: Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States; Biochemistry, Cell and Molecular Biology Program, Weill Cornell Graduate School of Medical Sciences, New York, NY, United States.
authors.forename: V
authors.initials: V
authors.lastname: Garg
authors.affiliation: Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States.
authors.forename: S
authors.initials: S
authors.lastname: Morgani
authors.affiliation: Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States; Biochemistry, Cell and Molecular Biology Program, Weill Cornell Graduate School of Medical Sciences, New York, NY, United States. Electronic address: hadj@mskcc.org.
authors.forename: A-K
authors.initials: AK
authors.lastname: Hadjantonakis
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D019070:Cell Lineage; D004475:Ectoderm / Q000166:cytology; D004707:Endoderm / Q000166:cytology; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology*
keywords: Embryonic stem cells; Epiblast stem cells; Extraembryonic stem cells; Preimplantation development; Trophoblast stem cells