title: Immunochromatographic Strip Assay for Detection ofCronobacter sakazakiiin Pure Culture.
abstract: Cronobacter sakazakii(C. sakazakii) is a foodborne pathogen, posing a high risk of disease to infants and immunocompromised individuals. In order to develop a quick, easy, and sensitive assay for detectingC. sakazakii, a rabbit anti-C. sakazakiiimmunoglobulin G (IgG) was developed using sonicated cell protein fromC. sakazakii. The developed anti-C. sakazakii(IgG) was of good quality and purity, as well as species-specific. The developed rabbit anti-C. sakazakiiIgG was attached to the surface of a sulforhodamine B-encapsulated liposome to form an immunoliposome. A test strip was then prepared by coating goat anti-rabbit IgG onto the control line and rabbit anti-C. sakazakiiIgG onto the test line, respectively, of a plastic-backed nitrocellulose membrane. A purple color signal both on the test line and the control line indicated the presence ofC. sakazakiiin the sample, whereas purple color only on the control line indicated the absence ofC. sakazakiiin the sample. This immunochromatographic strip assay could produce results in 15 min with a limit of detection of 107CFU/ml inC. sakazakiiculture. The immunochromatographic strip assay also showed very good specificity without cross-reactivity with other testedCronobacterspecies. Based on these results, the developed immunochromatographic strip assay is efficient for the detection ofC. sakazakiiand has high potential for on-site detection.
authors.affiliation: Department of Food Science and Technology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Food Science and Technology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
authors.forename: Shruti
authors.initials: S
authors.lastname: Shukla
authors.affiliation: Department of Food Science and Technology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
authors.forename: Gibaek
authors.initials: G
authors.lastname: Lee
authors.affiliation: Department of Food Science and Technology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
authors.forename: Myunghee
authors.initials: M
authors.lastname: Kim
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D044083:Cronobacter sakazakii / Q000276:immunology / Q000302:isolation & purification*; D003429:Cross Reactions; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D011817:Rabbits
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G
keywords: Antibody; Cronobacter sakazakii; immunochromatographic strip assay; liposome; rapid detection