title: Cultivation of Nannochloropsis oceanica biomass rich in eicosapentaenoic acid utilizing wastewater as nutrient resource.
abstract: The eicosapentaenoic acid rich marine eustigmatophyte Nannochloropsis oceanica was grown in wastewaters sampled from four different industries (i.e. pesticides industry, pharmaceutical industry, activated sludge treatment plant of municipality sewage and petroleum (oil) industry). Under the wastewater based growth conditions used in this study, the biomass productivity ranged from 21.78±0.87 to 27.78±0.22mgL(-1)d(-1) in relation to freeze dried biomass, while the lipid productivity varied between 5.59±0.02 and 6.81±0.04mgL(-1)d(-1). Although comparatively higher biomass, lipid and EPA productivity was observed in Conway medium, the %EPA content was similarly observed in pesticides industry and municipal effluents. The results highlight the possibility of selectively using wastewater as a growth medium, demonstrating the elevated eicosapentaenoic acid content and biodiesel properties, that complies with the European standards for biodiesel.
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Madhusree
authors.initials: M
authors.lastname: Mitra
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Freny
authors.initials: F
authors.lastname: Shah
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: S V Vamsi
authors.initials: SV
authors.lastname: Bharadwaj
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Shailesh Kumar
authors.initials: SK
authors.lastname: Patidar
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. Electronic address: smishra@csmcri.org.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Mishra
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D015118:Eicosapentaenoic Acid / Q000737:chemistry*; D058086:Microalgae / Q000737:chemistry* / Q000254:growth & development; D012722:Sewage / Q000737:chemistry; D058009:Stramenopiles / Q000737:chemistry / Q000254:growth & development; D062065:Wastewater / Q000382:microbiology*
chemical_list: D056804:Biofuels; D015118:Eicosapentaenoic Acid; D012722:Sewage; D062065:Wastewater
keywords: Biodiesel properties; Eicosapentaenoic acid; Nannochloropsis oceanica; Principal component analysis; Wastewater