title: Impact of two different colistin dosing strategies on healthy piglet fecal microbiota.
abstract: Colistin is often used in piglets but underdosing and overdosing are frequent. The impact of such administrations on fecal microbiota was studied. Piglets were given either underdoses of colistin by oral gavage for five days or overdoses by in-feed medication for 14days. The composition of fecal microbiota was studied by quantitative PCR, 16S rRNA sequencing, culture of Enterobacteriaceae, and quantification of short-chain fatty acids (SCFAs). The mean colistin concentrations during the treatment for underdosed and overdosed groups were 14.4μg/g and 64.9μg/g of feces respectively. Whatever the piglet and the sampling day, the two main phyla were Firmicutes and Bacteroidetes, The main families were Lactobacillaceae, Clostridiales, Lachnospiraceae and Ruminococcaceae. The main perturbation was the significant but transitory decrease in the Escherichia coli population during treatment, yet all the E. coli isolates were susceptible to colistin. Moreover, colistin did not affect the production of SCFAs. These results show that under- or overdoses of colistin do not result in any major disturbance of piglet fecal microbiota and rarely select for chromosomal resistance in the dominant E. coli population.
authors.affiliation: ANSES, Laboratoire de Ploufragan-Plouzané, Ploufragan, France; Université Bretagne, Loire, France; Clermont Université, Université d'Auvergne, Centre de Recherche en Nutrition Humaine Auvergne, EA 4678 CIDAM, Conception, Ingénierie et Développement de l'Aliment et du Médicament, Clermont-Ferrand, France.
authors.forename: M A
authors.initials: MA
authors.lastname: Fleury
authors.affiliation: ANSES, Laboratoire de Ploufragan-Plouzané, Ploufragan, France; Université Bretagne, Loire, France.
authors.forename: E
authors.initials: E
authors.lastname: Jouy
authors.affiliation: ANSES, Laboratoire de Ploufragan-Plouzané, Ploufragan, France; Université Bretagne, Loire, France.
authors.forename: F
authors.initials: F
authors.lastname: Eono
authors.affiliation: ANSES, Laboratoire de Ploufragan-Plouzané, Ploufragan, France; Université Bretagne, Loire, France.
authors.forename: R
authors.initials: R
authors.lastname: Cariolet
authors.affiliation: Inserm U1070, Pôle Biologie Santé, Poitiers, France; Université de Poitiers, UFR Médecine-Pharmacie, Poitiers, France; CHU Poitiers, Service de Toxicologie-Pharmacocinétique, Poitiers, France.
authors.forename: W
authors.initials: W
authors.lastname: Couet
authors.affiliation: CHU Poitiers, Service de Toxicologie-Pharmacocinétique, Poitiers, France.
authors.forename: P
authors.initials: P
authors.lastname: Gobin
authors.affiliation: Clermont Université, Université d'Auvergne, Centre de Recherche en Nutrition Humaine Auvergne, EA 4678 CIDAM, Conception, Ingénierie et Développement de l'Aliment et du Médicament, Clermont-Ferrand, France.
authors.forename: O
authors.initials: O
authors.lastname: Le Goff
authors.affiliation: Clermont Université, Université d'Auvergne, Centre de Recherche en Nutrition Humaine Auvergne, EA 4678 CIDAM, Conception, Ingénierie et Développement de l'Aliment et du Médicament, Clermont-Ferrand, France.
authors.forename: S
authors.initials: S
authors.lastname: Blanquet-Diot
authors.affiliation: Clermont Université, Université d'Auvergne, Centre de Recherche en Nutrition Humaine Auvergne, EA 4678 CIDAM, Conception, Ingénierie et Développement de l'Aliment et du Médicament, Clermont-Ferrand, France.
authors.forename: M
authors.initials: M
authors.lastname: Alric
authors.affiliation: ANSES, Laboratoire de Ploufragan-Plouzané, Ploufragan, France; Université Bretagne, Loire, France. Electronic address: isabelle.kempf@anses.fr.
authors.forename: I
authors.initials: I
authors.lastname: Kempf
mesh_terms: D000818:Animals; D003091:Colistin / Q000008:administration & dosage / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013552:Swine / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S; D003091:Colistin
keywords: Colistin; E. coli; Fecal microbiota; Piglet; Resistance