title: Acclimation capacity of the three-spined stickleback (Gasterosteus aculeatus, L.) to a sudden biological stress following a polymetallic exposure.
abstract: To get closer to the environmental reality, ecotoxicological studies should no longer consider the evaluation of a single pollutant, but rather combination of stress and their interaction. The aim of this study was to determine if responses of a fish to a sudden biological stress could be modified by a prior exposure to a chemical stress (a polymetallic contamination). For this purpose, in situ experiment was conducted in three ponds in the Haute-Vienne department (France). One pond was chosen for its high uranium concentration due to uranium mine tailings, and the two other ponds, which were not submitted to these tailings. Three-spined sticklebacks (Gasterosteus aculeatus) were caged in these ponds for 14 days. After this period, fish were submitted to a biological stress, exerted by lipopolysaccharides injection after anesthesia, and were sacrificed 4 days after these injections for multi-biomarkers analyses (leucocyte viability, phagocytic capacity and reactive oxygen species production, antioxidant peptide and enzymes, lipid peroxidation and DNA damage). The pond which received uranium mine tailings had higher metallic concentrations. Without biological stress, sticklebacks caged in this pond presented an oxidative stress, with increasing of reactive oxygen species levels, modification of some parts of the antioxidant system, and lipid peroxidation. Caging in the two most metal-contaminated ponds resulted in an increase of susceptibility of sticklebacks to the biological stress, preventing their phagocytic responses to lipopolysaccharides and modifying their glutathione contents and glutathione-S-transferase activity.
authors.affiliation: UMR-I 02 SEBIO (Stress Environnementaux et Biosurveillance des milieux aquatiques), Institut National de l'Environnement Industriel et des Risques (INERIS), BP 2, 60550, Verneuil-En-Halatte, France. antoineleguernic@gmail.com.|Centre de Cadarache, PRP-ENV/SERIS/LECO, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), 13115, Saint-Paul-Lez-Durance, France. antoineleguernic@gmail.com.|UFR Sciences Exactes et Naturelles, UMR-I 02 SEBIO, Université de Reims Champagne-Ardenne (URCA), Campus Moulin de la Housse, B.P. 1039, 51687, Reims, France. antoineleguernic@gmail.com.
authors.forename: Antoine
authors.initials: A
authors.lastname: Le Guernic
authors.affiliation: UMR-I 02 SEBIO (Stress Environnementaux et Biosurveillance des milieux aquatiques), Institut National de l'Environnement Industriel et des Risques (INERIS), BP 2, 60550, Verneuil-En-Halatte, France.
authors.forename: Wilfried
authors.initials: W
authors.lastname: Sanchez
authors.affiliation: UMR-I 02 SEBIO (Stress Environnementaux et Biosurveillance des milieux aquatiques), Institut National de l'Environnement Industriel et des Risques (INERIS), BP 2, 60550, Verneuil-En-Halatte, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Palluel
authors.affiliation: UMR-I 02 SEBIO (Stress Environnementaux et Biosurveillance des milieux aquatiques), Institut National de l'Environnement Industriel et des Risques (INERIS), BP 2, 60550, Verneuil-En-Halatte, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Bado-Nilles
authors.affiliation: Centre de Cadarache, PRP-ENV/SERIS/LECO, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), 13115, Saint-Paul-Lez-Durance, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Floriani
authors.affiliation: UMR-I 02 SEBIO (Stress Environnementaux et Biosurveillance des milieux aquatiques), Institut National de l'Environnement Industriel et des Risques (INERIS), BP 2, 60550, Verneuil-En-Halatte, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Turies
authors.affiliation: UMR-I 02 SEBIO (Stress Environnementaux et Biosurveillance des milieux aquatiques), Institut National de l'Environnement Industriel et des Risques (INERIS), BP 2, 60550, Verneuil-En-Halatte, France.
authors.forename: Edith
authors.initials: E
authors.lastname: Chadili
authors.affiliation: Centre de Cadarache, PRP-ENV/SERIS/LECO, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), 13115, Saint-Paul-Lez-Durance, France.
authors.forename: Claire Della
authors.initials: CD
authors.lastname: Vedova
authors.affiliation: Centre de Cadarache, PRP-ENV/SERIS/LECO, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), 13115, Saint-Paul-Lez-Durance, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Cavalié
authors.affiliation: Centre de Cadarache, PRP-ENV/SERIS/LECO, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), 13115, Saint-Paul-Lez-Durance, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Adam-Guillermin
authors.affiliation: UMR-I 02 SEBIO (Stress Environnementaux et Biosurveillance des milieux aquatiques), Institut National de l'Environnement Industriel et des Risques (INERIS), BP 2, 60550, Verneuil-En-Halatte, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Porcher
authors.affiliation: UFR Sciences Exactes et Naturelles, UMR-I 02 SEBIO, Université de Reims Champagne-Ardenne (URCA), Campus Moulin de la Housse, B.P. 1039, 51687, Reims, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Geffard
authors.affiliation: UFR Sciences Exactes et Naturelles, UMR-I 02 SEBIO, Université de Reims Champagne-Ardenne (URCA), Campus Moulin de la Housse, B.P. 1039, 51687, Reims, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Betoulle
authors.affiliation: Centre de Cadarache, PRP-ENV/SERIS/LECO, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), 13115, Saint-Paul-Lez-Durance, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Gagnaire
mesh_terms: D000064:Acclimatization; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004784:Environmental Monitoring; D005602:France; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation; D008670:Metals / Q000633:toxicity*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D023701:Smegmamorpha / Q000502:physiology*; D013312:Stress, Physiological; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D008670:Metals; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D005978:Glutathione
keywords: Acclimation capacity; Biological stress; Caging; Chemical stress; Lipopolysaccharides; Polymetallic contamination; Three-spined stickleback