title: MicroRNA-203 As a Stemness Inhibitor of Glioblastoma Stem Cells.
abstract: Glioblastoma stem cells (GBM-SCs) are believed to be a subpopulation within all glioblastoma (GBM) cells that are in large part responsible for tumor growth and the high grade of therapeutic resistance that is so characteristic of GBM. MicroRNAs (miR) have been implicated in regulating the expression of oncogenes and tumor suppressor genes in cancer stem cells, including GBM-SCs, and they are a potential target for cancer therapy. In the current study, miR-203 expression was reduced in CD133(+) GBM-SCs derived from six human GBM biopsies. MicroRNA-203 transfected GBM-SCs had reduced capacity for self-renewal in the cell sphere assay and increased expression of glial and neuronal differentiation markers. In addition, a reduced proliferation rate and an increased rate of apoptosis were observed. Therefore, miR-203 has the potential to reduce features of stemness, specifically in GBM-SCs, and is a logical target for GBM gene therapy.
authors.affiliation: Department of Neurosurgery, Huizhou Municipal Central Hospital, Huizhou City, Guangdong Province, 516000, The People's Republic of China.|Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, The People's Republic of China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Neurosurgery, Huizhou Municipal Central Hospital, Huizhou City, Guangdong Province, 516000, The People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, Huizhou Municipal Central Hospital, Huizhou City, Guangdong Province, 516000, The People's Republic of China.
authors.forename: Honghai
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, The People's Republic of China.
authors.forename: Shiguang
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000071916:AC133 Antigen / Q000378:metabolism; D000328:Adult; D017209:Apoptosis / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics / Q000628:therapy*; D063646:Carcinogenesis / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D000066673:Cell Self Renewal / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015316:Genetic Therapy; D005909:Glioblastoma / Q000235:genetics / Q000628:therapy*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D000071916:AC133 Antigen; C081876:CKAP4 protein, human; C546703:MIRN203 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs
keywords: differentiation; glioblastoma stem cells; miR-203; self-renewal ability