title: Evaluation of the Antioxidant Activity of Aqueous and Methanol Extracts of Pleurotus ostreatus in Different Growth Stages.
abstract: Total polyphenols and flavonoids contents, as well as ferric reducing antioxidant power (FRAP), metal ions chelating activity, reducing power assay and scavenging activity of DPPH and ABTS radicals in aqueous and methanolic extracts obtained from mycelium, primordium, and fruiting body of Pleurotus ostreatus in both fresh as dry, were evaluated. The total polyphenol content of dried samples was higher in aqueous extracts obtained both in room temperature and boiling. The total polyphenol content of the fresh samples obtained at room temperature and boiling was higher in aqueous extract of mycelium and in the methanolic extract of the fruiting body. In general, flavonoids represented a very small percentage of the total polyphenol content. The antioxidant activity measured by the FRAP method of extracts from fresh samples were higher with respect to the dried samples. The results of the metal ion chelating activity indicate that all extracts tested had acted. The reducing power of all samples was concentration dependent. In general, the extracts of dried samples showed higher reducing power than the extracts of fresh samples and tend to show greater reducing power by aqueous than methanolic extracts. It was observed that the DPPH and ABTS radical scavenging activities were positively correlated to the concentration of the extract. The results suggested that antioxidant activity could be due to polyphenols, but mainly by different molecules or substances present in the extracts. Overall, the fruiting body of P. ostreatus showed the best results and the possibility of continuing to investigate its functional properties of this fungus is opened. This is the first report where the antioxidant activity of P. ostreatus in different growth stage was reported.
authors.affiliation: Department of Biotechnology, Metropolitan Autonomous University, Campus IztapalapaMexico, Mexico; Laboratory of Biotechnology, Research Center for Biological Sciences, Autonomous University of TlaxcalaTlaxcala, Mexico.
authors.forename: Ivette
authors.initials: I
authors.lastname: González-Palma
authors.affiliation: Department of Biotechnology, Metropolitan Autonomous University, Campus Iztapalapa Mexico, Mexico.
authors.forename: Héctor B
authors.initials: HB
authors.lastname: Escalona-Buendía
authors.affiliation: Department of Biotechnology, Metropolitan Autonomous University, Campus Iztapalapa Mexico, Mexico.
authors.forename: Edith
authors.initials: E
authors.lastname: Ponce-Alquicira
authors.affiliation: Laboratory of Mycology, Biological Research Center, Autonomous University of Morelos State Morelos, Mexico.
authors.forename: Maura
authors.initials: M
authors.lastname: Téllez-Téllez
authors.affiliation: Molecular Glycobiotechnology Group, Discipline of Biochemistry, National University of Ireland Galway Galway, Ireland.
authors.forename: Vijai K
authors.initials: VK
authors.lastname: Gupta
authors.affiliation: Laboratory of Biotechnology, Research Center for Biological Sciences, Autonomous University of Tlaxcala Tlaxcala, Mexico.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Díaz-Godínez
authors.affiliation: Department of Biotechnology, Metropolitan Autonomous University, Campus Iztapalapa Mexico, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Soriano-Santos
keywords: Pleurotus ostreatus; antioxidant activity; chelating activity; growth stages; polyphenols compounds