title: Ginsenoside Rb1 alleviates aluminum chloride-induced rat osteoblasts dysfunction.
abstract: Osteoblasts dysfunction, induced by aluminum (Al), plays a critical role in the osteoporosis etiology. Ginsenoside Rb1 (Rb1) has the therapeutic properties for osteoporosis. This study aimed to assess the efficiency of Rb1 in ameliorating Al-induced osteoblasts dysfunction. The osteoblasts were divided into four groups: Rb1-treated group (RG, 0.0145mg/mL Rb1), control group (CG, 0), AlCl3-treated group (AG, 0.126mg/mL AlCl3·6H2O), AlCl3+Rb1-treated group (ARG, 0.0145mg/mL Rb1 and 0.126mg/mL AlCl3·6H2O). After 24h of culture, the osteoblasts viability, the transforming growth factor-β1 (TGF-β1), bone morphogenetic protein-2 (BMP-2), the insulin-like growth factor I (IGF-I), core-binding factor α1 (Cbfα1) mRNA expressions, glutathione perioxidase (GSH-Px)and superoxide dismutase (SOD) activities, and reactive oxygen species (ROS) concentration were determined. The osteoblasts ultrastructural features were also observed. In the ARG, the osteoblasts viability, TGF-β1, BMP-2, IGF-I and Cbfα1 mRNA expressions and the GSH-Pxand SOD activities were significantly increased, the ROS concentration was significantly decreased, and osteoblasts histology lesion was attenuated compared with the AG. These results demonstrated that Rb1 could significantly reverse osteoblasts viability and osteoblasts growth regulation factor, inhibit oxidative stress, and attenuate histology lesion in the osteoblasts with AlCl3. These results indicate that Rb1 can effectively alleviate the AlCl3-induced osteoblasts dysfunction.
authors.affiliation: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China. Electronic address: zhuyanzhu@caas.cn.
authors.forename: Yanzhu
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: College of Animals Science, Fujian Agricultural and Forestry University, Fuzhou 350002, China.
authors.forename: Chongwei
authors.initials: C
authors.lastname: Hu
authors.affiliation: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China.
authors.forename: Peihe
authors.initials: P
authors.lastname: Zheng
authors.affiliation: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China.
authors.forename: Liguang
authors.initials: L
authors.lastname: Miao
authors.affiliation: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China.
authors.forename: Xijun
authors.initials: X
authors.lastname: Yan
authors.affiliation: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.affiliation: Vascular Surgery Department, the First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Zhongying
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address: liyanfei@neau.edu.cn.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Li
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002712:Chlorides / Q000633:toxicity*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D036145:Ginsenosides / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010024:Osteoporosis / Q000139:chemically induced / Q000188:drug therapy; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D017607:Aluminum Compounds; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D002712:Chlorides; D050659:Core Binding Factor Alpha 1 Subunit; D036145:Ginsenosides; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D053773:Transforming Growth Factor beta1; D000077410:Aluminum Chloride; D007334:Insulin-Like Growth Factor I; C442759:ginsenoside Rb1; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Aluminum; Growth regulation factors; Osteoblasts viability; Oxidative stress; Ultrastructural features