title: Structural and Dynamic Characterization of the Molecular Hub Early Region 1A (E1A) from Human Adenovirus.
abstract: The small-DNA human adenovirus encodes one of the most versatile molecular hubs, the E1A protein. This protein is essential for productive viral infection in human cells and a vast amount of biologically relevant data are available on its interactions with host proteins. Up to now, however, no high-resolution structural and dynamic information on E1A is available despite its important biological role. Among the different spliced variants of E1A, two are expressed at high level in the early stage of infection. These are 243 and 289 residues isoforms. Herein, we present their NMR characterization, showing that they are both highly disordered, but also demonstrate a certain heterogeneous behavior in terms of structural and dynamic properties. Furthermore, we present the characterization of the isolated domain of the longer variant, known as CR3. This study opens the way to understanding at the molecular level how E1A functions.
authors.affiliation: CERM and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy.
authors.forename: Tomáš
authors.identifier: http://orcid.org/0000-0001-8091-2648
authors.initials: T
authors.lastname: Hošek
authors.affiliation: CERM and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy.
authors.forename: Eduardo O
authors.initials: EO
authors.lastname: Calçada
authors.affiliation: CERM and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy.
authors.forename: Marcela Oliveira
authors.initials: MO
authors.lastname: Nogueira
authors.affiliation: CERM and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Salvi
authors.affiliation: CERM and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy.
authors.forename: Talita Duarte
authors.initials: TD
authors.lastname: Pagani
authors.affiliation: CERM and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy. felli@cerm.unifi.it.
authors.forename: Isabella C
authors.initials: IC
authors.lastname: Felli
authors.affiliation: CERM and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy. pierattelli@cerm.unifi.it.
authors.forename: Roberta
authors.identifier: http://orcid.org/0000-0001-7755-0885
authors.initials: R
authors.lastname: Pierattelli
mesh_terms: D017500:Adenovirus E1A Proteins / Q000737:chemistry*; D000260:Adenoviruses, Human / Q000737:chemistry*; D006801:Humans; D066329:Protein Aggregates; D000072417:Protein Domains; D020033:Protein Isoforms / Q000737:chemistry
chemical_list: D017500:Adenovirus E1A Proteins; D066329:Protein Aggregates; D020033:Protein Isoforms
keywords: NMR spectroscopy; human adenovirus; intrinsically disordered proteins (IDP); molecular hub; viral proteins