title: A functional chitosan membrane with grafted epigallocatechin-3-gallate and lovastatin enhances periodontal tissue regeneration in dogs.
abstract: Currently used guided tissue regeneration (GTR) membranes are mainly used as a barrier to prevent epithelial cells growth into defects before new bone formation. The aim of this study was to develop a tri-layer functional chitosan (CS) membrane with epigallocatechin-3-gallate (EGCG) grafted on the outer layer for bactericidal activity, and lovastatin was included in the middle layer for controlled release. Successful EGCG grafting was demonstrated using Fourier transform infrared spectroscopy and EGCG grafting significantly enhanced adhesion and proliferation of human gingival fibroblasts. The release duration of lovastatin reached 21days. CS-Lovastatin1 produced the highest alkaline phosphatase activity and EGCG14-CS exhibited the best bactericidal activity against periodontopathic bacteria. Finally, the EGCG14-CS-Lovastatin1 membrane showed a higher percentage of bone regeneration than BioMend(®) and control groups in one-walled defects of beagle dogs. These results suggest that the EGCG14-CS-Lovastatin1 membrane has the potential to be used as a novel GTR membrane.
authors.affiliation: Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University and National Taiwan University Hospital, Taipei 10048, Taiwan. Electronic address: leebs@ntu.edu.tw.
authors.forename: Bor-Shiunn
authors.initials: BS
authors.lastname: Lee
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University and National Taiwan University Hospital, Taipei 10048, Taiwan. Electronic address: zionist35@hotmail.com.
authors.forename: Chien-Chen
authors.initials: CC
authors.lastname: Lee
authors.affiliation: Department of Dentistry, MacKay Memorial Hospital, Taipei 10449, Taiwan; Department of Dentistry, School of Dentistry, National Taiwan University, Taipei 10048, Taiwan. Electronic address: hungpin@gmail.com.
authors.forename: Hung-Pin
authors.initials: HP
authors.lastname: Lin
authors.affiliation: Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University and National Taiwan University Hospital, Taipei 10048, Taiwan. Electronic address: v79319@hotmail.com.
authors.forename: Wei-An
authors.initials: WA
authors.lastname: Shih
authors.affiliation: Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University and National Taiwan University Hospital, Taipei 10048, Taiwan. Electronic address: r00450017@ntu.edu.tw.
authors.forename: Wan-Ling
authors.initials: WL
authors.lastname: Hsieh
authors.affiliation: College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. Electronic address: chern@kmu.edu.tw.
authors.forename: Chern-Hsiung
authors.initials: CH
authors.lastname: Lai
authors.affiliation: Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8549, Japan. Electronic address: takeuchi.peri@tmd.ac.jp.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Takeuchi
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University and National Taiwan University Hospital, Taipei 10048, Taiwan. Electronic address: yiwenchen@ntu.edu.tw.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004285:Dogs; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D065546:Drug Liberation; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000187:drug effects; D048091:Guided Tissue Regeneration; D006801:Humans; D008148:Lovastatin / Q000737:chemistry*; D008297:Male; D008567:Membranes, Artificial; D013718:Tensile Strength
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D008567:Membranes, Artificial; D002392:Catechin; D048271:Chitosan; D008148:Lovastatin; C045651:epigallocatechin gallate; D000469:Alkaline Phosphatase
keywords: Chitosan; Collagen membrane; Epigallocatechin-3-gallate; Guided tissue regeneration; Lovastatin; Periodontitis