title: Different expression levels of glycans on leukemic cells-a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid.
abstract: Sialic acid (SA) is normally expressed on the cell membranes and is located at the terminal position of the sugar chains. SA plays an important role for regulation of the innate immunity, function as markers of the cells and can be recognized by a variety of receptors. Interestingly, the level of SA expression is increased on metastatic cancer cells. The availability of specific antibodies against SA is limited and, therefore, biomarker tools for detection of SA are lacking. We have recently presented a novel method for specific fluorescence labeling of SA molecular imprinted polymers (MIP). Here, we have performed an extended screening of SA expression by using SA-MIP and included four different chronic lymphocytic leukemia (CLL) cell lines, conveniently analyzed by flow cytometry and fluorescence microscopy. SA expression was detected in four cell lines at different levels, and the SA expression were verified with lectin-FITC. These results show that SA-MIP can be used as a plastic antibody for detection of SA using both flow cytometry and fluorescence microscopy. We suggest that SA-MIP can be used for screening of different tumor cells of various stages, including CLL cells.
authors.affiliation: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Malmö, Sweden. Zahra.el-schich@mah.se.
authors.forename: Zahra
authors.initials: Z
authors.lastname: El-Schich
authors.affiliation: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Malmö, Sweden.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Abdullah
authors.affiliation: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Malmö, Sweden.
authors.forename: Sudhirkumar
authors.initials: S
authors.lastname: Shinde
authors.affiliation: Department of Translational Medicine, Lund University, Malmö, Sweden.
authors.forename: Nishtman
authors.initials: N
authors.lastname: Dizeyi
authors.affiliation: Department of Clinical and Experimental Medicine, Division of Cell Biology, Linköping University, Linköping, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Rosén
authors.affiliation: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Malmö, Sweden.
authors.forename: Börje
authors.initials: B
authors.lastname: Sellergren
authors.affiliation: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Malmö, Sweden.
authors.forename: Anette Gjörloff
authors.identifier: http://orcid.org/0000-0002-2993-0354
authors.initials: AG
authors.lastname: Wingren
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D005453:Fluorescence; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000378:metabolism; D054802:Molecular Imprinting; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011134:Polysaccharides / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D011108:Polymers; D011134:Polysaccharides; D019158:N-Acetylneuraminic Acid
keywords: Chronic lymphocytic leukemia; Lectin; Molecular imprinting polymers; Sialic acid