title: A Female-Biased Odorant Receptor from Apolygus lucorum (Meyer-Dür) Tuned to Some Plant Odors.
abstract: Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae) is a serious pest of cotton, jujube, grape and many other crops around the world. Understanding how olfactory information directs this insect to its host plants may provide environment-friendly approaches to the control of its population in agriculture. In our study, we cloned an odorant receptor gene, AlucOR46, that was specifically expressed in antennae and female-biased. Functional expression of AlucOR46 in Xenopus oocytes showed that it is tuned to six plant volatiles (S)-(-)-Limonene, (R)-(+)-Limonene, (E)-2-Hexenal, (E)-3-Hexenol, 1-Heptanol and (1R)-(-)-Myrtenol. Electroantennogram (EAG) recordings revealed that all six compounds could elicit electrophysiological responses from the antennae of A. lucorum, higher in females. Our results are in agreement with previous reports showing that (E)-2-Hexenal could attract female A. lucorum in behavior experiments. These results suggest that AlucOR46 might play an important role in locating the host plants of A. lucorum and therefore represents a suitable target for green pest control.
authors.affiliation: School of Life Science, Shanxi Normal University, Linfen 041000, China. zhang3121414@126.com.|State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. zhang3121414@126.com.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Life Science, Shanxi Normal University, Linfen 041000, China. zhangmp2006@163.com.
authors.forename: Meiping
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. ysw_0220@163.com.
authors.forename: Shuwei
authors.initials: S
authors.lastname: Yan
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. grwang@ippcaas.cn.
authors.forename: Guirong
authors.initials: G
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. yangliu@ippcaas.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000447:Aldehydes / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D055724:Electrophysiological Phenomena; D005260:Female; D020062:Heteroptera / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D009812:Odorants / Q000032:analysis*; D010944:Plants / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014835:Volatilization
chemical_list: D000447:Aldehydes; D019476:Insect Proteins; D018035:Receptors, Odorant; C051750:2-hexenal
keywords: Apolygus lucorum; Xenopus oocytes; electroantennogram; odorant receptor; plant volatile