title: HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland.
abstract: Versican expression promotes tumor growth by destabilizing focal cell contacts, thus impeding cell adhesion and facilitating cell migration. It not only presents or recruits molecules to the cell surface, but also modulates gene expression levels and coordinates complex signal pathways. Previously, we suggested that the interaction between versican and human epidermal growth factor receptors may be directly associated with tumor aggressiveness. Thus, the expression of EGFR and HER-2 in these neoplasms may contribute to a better understanding of the progression mechanisms in malignant mammary tumors. The purpose of this study was to correlate the gene and protein expressions of EGFR and HER2 by RNA In Situ Hybridization (ISH) and immunohistochemistry (IHC), respectively, and their relationship with the versican expression in carcinomas in mixed tumors and carcinosarcomas of the canine mammary gland. The results revealed that EGFR mRNA expression showed a significant difference between in situ and invasive carcinomatous areas in low and high versican expression groups. Identical results were observed in HER-2 mRNA expression. In immunohistochemistry analysis, neoplasms with low versican expression showed greater EGFR immunostaining in the in situ areas than in invasive areas, even as the group presenting high versican expression displayed greater EGFR and HER-2 staining in in situ areas. Significant EGFR and HER-2 mRNA and protein expressions in in situ carcinomatous sites relative to invasive areas suggest that these molecules play a role during the early stages of tumor progression.
authors.affiliation: Department of General Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Mina Gerais, Brazil.|Laboratory of Experimental Pathology, Gonçalo Moniz Institute, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil.
authors.forename: Karine Araújo
authors.initials: KA
authors.lastname: Damasceno
authors.affiliation: Department of General Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Mina Gerais, Brazil.
authors.forename: Enio
authors.initials: E
authors.lastname: Ferreira
authors.affiliation: Department of Pathology and Clinics, School of Veterinary Medicine and Zootechny, Universidade Federal da Bahia, Salvador, Bahia, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Estrela-Lima
authors.affiliation: Department of General Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Mina Gerais, Brazil.
authors.forename: Conrado de Oliveira
authors.initials: Cde O
authors.lastname: Gamba
authors.affiliation: Department of General Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Mina Gerais, Brazil.
authors.forename: Fernanda Freitas
authors.initials: FF
authors.lastname: Miranda
authors.affiliation: International Center of Research in Cancer (CIPE), A.C. Camargo Cancer Center, São Paulo, São Paulo, Brazil.
authors.forename: Mariana Rezende
authors.initials: MR
authors.lastname: Alves
authors.affiliation: International Center of Research in Cancer (CIPE), A.C. Camargo Cancer Center, São Paulo, São Paulo, Brazil.
authors.forename: Rafael Malagoli
authors.initials: RM
authors.lastname: Rocha
authors.affiliation: Department of Clinical and Toxicological Analyses, School of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: André Luís Branco
authors.initials: AL
authors.lastname: de Barros
authors.affiliation: Department of General Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Mina Gerais, Brazil.
authors.forename: Geovanni Dantas
authors.initials: GD
authors.lastname: Cassali
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D066246:ErbB Receptors / Q000235:genetics; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015674:Mammary Neoplasms, Animal / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D066246:ErbB Receptors; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D018719:Erb-b2 Receptor Tyrosine Kinases