title: Extracellular Proteome Profiling ofBacillus pumilusSCU11 Producing Alkaline Protease for Dehairing.
abstract: Bacillus pumilusis one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. A novelB. pumilusSCU11 strain possessing high alkaline protease activity was obtained in our previous work. The culture supernatant of this strain showed efficient dehairing capability with minimal collagen damage, indicating promising potential applications in the leather industry. In this study, the strain's extracellular proteome was identified by LC-MS/MS-based shotgun proteomic analysis, and their related secretory pathways were characterized by BLAST searches. A total of 513 proteins, including 100 actual secreted and 413 intracellular proteins, were detected in the extracellular proteome. The functions of these secreted proteins were elucidated and four complete secretory systems (Sec, Tat, Com, and ABC transporter) were proposed forB. pumilus. These data provideB. pumilusa comprehensive extracellular proteome profile, which is a valuable theoretical and applicative basis for future genetic modifications and development of industrial enzymes.
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, P.R. China.
authors.forename: Shiqiang
authors.initials: S
authors.lastname: Yu
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Song
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, P.R. China.
authors.forename: Tingting
authors.initials: T
authors.lastname: He
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, P.R. China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Shao
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, P.R. China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
mesh_terms: D000069979:Bacillus pumilus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005110:Extracellular Space / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D006197:Hair / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D010450:Endopeptidases; C087822:alkaline protease
keywords: Bacillus pumilus; protease; secretome; secretory pathway