title: Single-Cell RNAseq Reveals That Pancreatic β-Cells From Very Old Male Mice Have a Young Gene Signature.
abstract: Aging improves pancreatic β-cell function in mice. This is a surprising finding because aging is typically associated with functional decline. We performed single-cell RNA sequencing of β-cells from 3- and 26-month-old mice to explore how changes in gene expression contribute to improved function with age. The old mice were healthy and had reduced blood glucose levels and increased β-cell mass, which correlated to their body weight. β-Cells from young and old mice had similar transcriptome profiles. In fact, only 193 genes (0.89% of all detected genes) were significantly regulated (≥2-fold; false discovery rate < 0.01; normalized counts > 5). Of these, 183 were down-regulated and mainly associated with pathways regulating gene expression, cell cycle, cell death, and survival as well as cellular movement, function, and maintenance. Collectively our data show that β-cells from very old mice have transcriptome profiles similar to those of young mice. These data support previous findings that aging is not associated with reduced β-cell mass or functional β-cell decline in mice.
authors.affiliation: Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591.
authors.forename: Haruka
authors.initials: H
authors.lastname: Okamoto
authors.affiliation: Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591.
authors.forename: Jinrang
authors.initials: J
authors.lastname: Kim
authors.affiliation: Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591.
authors.forename: Min
authors.initials: M
authors.lastname: Ni
authors.affiliation: Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591.
authors.forename: Christina
authors.initials: C
authors.lastname: Adler
authors.affiliation: Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591.
authors.forename: Katie
authors.initials: K
authors.lastname: Cavino
authors.affiliation: Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591.
authors.forename: Erqian
authors.initials: E
authors.lastname: Na
authors.affiliation: Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Murphy
authors.affiliation: Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591.
authors.forename: George D
authors.initials: GD
authors.lastname: Yancopoulos
authors.affiliation: Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591.
authors.forename: Calvin
authors.initials: C
authors.lastname: Lin
authors.affiliation: Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591.
authors.forename: Jesper
authors.initials: J
authors.lastname: Gromada
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D016922:Cellular Senescence; D005947:Glucose / Q000378:metabolism; D006706:Homeostasis; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D017423:Sequence Analysis, RNA; D059010:Single-Cell Analysis; D014157:Transcription Factors / Q000378:metabolism; D059467:Transcriptome
chemical_list: D014157:Transcription Factors; D005947:Glucose