title: Physiological characteristics and metabolomics of transgenic wheat containing the maize C4phosphoenolpyruvate carboxylase (PEPC) gene under high temperature stress.
abstract: In this paper, two transgenic wheat lines, PC27 and PC51, containing the maize PEPC gene and its wild-type (WT) were used as experimental material to study the effects of high temperature on their photosynthetic physiological characteristics and metabolome. The results showed that transgenic wheat lines had higher photosynthetic rate (Pn) than WT under non-stress treatment (NT) and high temperature stress treatment (HT), and more significantly under HT. The change trends of Fv/Fm, ФPSII, and qPwere similar to Pn, whereas that of non-photochemical quenching (NPQ) was the opposite. Compared with WT, no differences in chlorophyll content between the transgenic wheat and WT were observed under NT, but two transgenic lines had relatively higher contents than WT under HT. The change trends of Chlorophyll a/b radio, the decreased values of Fm, Wk, and Vj, and the activity of the antioxidant enzyme were consistent with the chlorophyll content. Compared with WT, transgenic wheat lines exhibited lower rate of superoxide anion production, H2O2and malondialdehyde content under HT, and no significant differences were observed under NT. The expression pattern of the ZmPEPC gene and wheat endogenous photosynthesis-related genes were in agreement with that of Pn. Compared with WT, about 13 different metabolites including one organic acid, six amino acids, four sugars, and two polyols were identified under NT; 25 different metabolites including six organic acids, 12 amino acids, four sugars, and three polyols were identified under HT. Collectively, our results indicate that ZmPEPC gene can enhance photochemical and antioxidant enzyme activity, upregulate the expression of photosynthesis-related genes, delay degradation of chlorophyll, change contents of proline and other metabolites in wheat, and ultimately improves its heat tolerance.
authors.affiliation: College of Agronomy, Henan Agricultural University, Zhengzhou, Henan, 450002, China.|Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Qi
authors.affiliation: Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China. xuwg1958@163.com.
authors.forename: Weigang
authors.initials: W
authors.lastname: Xu
authors.affiliation: Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.
authors.forename: Jianzhou
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.
authors.forename: Mingzhong
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Hu
authors.affiliation: Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.
authors.forename: Huiwei
authors.initials: H
authors.lastname: Wang
authors.affiliation: Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Dong
authors.affiliation: Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D005453:Fluorescence; D005740:Gases / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D055442:Metabolome; D055432:Metabolomics; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics*; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D013312:Stress, Physiological / Q000235:genetics*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014908:Triticum / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
chemical_list: D005740:Gases; D010940:Plant Proteins; D013481:Superoxides; D002734:Chlorophyll; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D010730:Phosphoenolpyruvate Carboxylase
keywords: Heat tolerance; Metabolomic; Phosphoenolpyruvate carboxylase (PEPC); Photosynthesis; Wheat