title: Deep sequencing reveals global patterns of mRNA recruitment during translation initiation.
abstract: In this work, we developed a method to systematically study the sequence preference of mRNAs during translation initiation. Traditionally, the dynamic process of translation initiation has been studied at the single molecule level with limited sequencing possibility. Using deep sequencing techniques, we identified the sequence preference at different stages of the initiation complexes. Our results provide a comprehensive and dynamic view of the initiation elements in the translation initiation region (TIR), including the S1 binding sequence, the Shine-Dalgarno (SD)/anti-SD interaction and the second codon, at the equilibrium of different initiation complexes. Moreover, our experiments reveal the conformational changes and regional dynamics throughout the dynamic process of mRNA recruitment.
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), and State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Gao
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), and State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yu
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), and State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Jukui
authors.initials: J
authors.lastname: Nie
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), and State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Tengfei
authors.initials: T
authors.lastname: Lian
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), and State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Jianshi
authors.initials: J
authors.lastname: Jin
authors.affiliation: Department of Biochemistry and Structural Biology, Lund University, Lund, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Liljas
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), and State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Su
mesh_terms: D001483:Base Sequence; D018387:Codon, Initiator / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D010442:Peptide Chain Initiation, Translational / Q000235:genetics*; D014176:Protein Biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D018387:Codon, Initiator; D012333:RNA, Messenger