title: Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays.
abstract: Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mean read length 289 ± 183 bp) detecting a total of 5399 contigs (mean contig length: 396 ± 91 bp). Mining this data set, a panel of 143 in silico SNPs was selected. Eighty-two of these SNPs were successfully validated and 61 were polymorphic: 14 in P. falkneri, 21 in P. motoro, 3 in both species and 26 fixed for alternative variants in both species, thus being useful for population analyses and hybrid detection.
authors.affiliation: Laboratório de Biologia e Genética de Peixes, Instituto de Biociências de Botucatu, UNESP, Botucatu, SP, 18618-970, Brazil.
authors.forename: Vanessa P
authors.initials: VP
authors.lastname: Cruz
authors.affiliation: Laboratori d'Ictiologia Genètica, Department of Biology, Faculty of Sciences, University of Girona, Campus de Montilivi, Girona, 17071, Spain.|Departamento de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, 27002, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Vera
authors.affiliation: Departamento de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, 27002, Spain.
authors.forename: Belén G
authors.initials: BG
authors.lastname: Pardo
authors.affiliation: Institute of Aquaculture, University of Stirling, Pathfoot Building, Stirling, FK9 4LA, UK.
authors.forename: John
authors.initials: J
authors.lastname: Taggart
authors.affiliation: Departamento de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, 27002, Spain.
authors.forename: Paulino
authors.initials: P
authors.lastname: Martinez
authors.affiliation: Laboratório de Biologia e Genética de Peixes, Instituto de Biociências de Botucatu, UNESP, Botucatu, SP, 18618-970, Brazil.
authors.forename: Claudio
authors.initials: C
authors.lastname: Oliveira
authors.affiliation: Laboratório de Biologia e Genética de Peixes, Instituto de Biociências de Botucatu, UNESP, Botucatu, SP, 18618-970, Brazil.
authors.forename: Fausto
authors.initials: F
authors.lastname: Foresti
mesh_terms: D000818:Animals; D020451:Contig Mapping; D005618:Fresh Water; D005819:Genetic Markers; D005828:Genetics, Population; D006824:Hybridization, Genetic; D020641:Polymorphism, Single Nucleotide; D012860:Skates, Fish / Q000235:genetics*
chemical_list: D005819:Genetic Markers
keywords: 
  Potamotrygon
  ; NGS techniques; SNPs; conservation tools; genetic fish; hybridization