title: Proteomic Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) Degradation and Detoxification inSphingobium chungbukenseDJ77.
abstract: Polycyclic aromatic hydrocarbons (PAHs) are commonly present xenobiotics in natural and contaminated soils. We studied three (phenanthrene, naphthalene, and biphenyl) xenobiotics, catabolism, and associated proteins inSphingobium chungbukenseDJ77 by two-dimensional gel electrophoresis (2-DE) analysis. Comparative analysis of the growth-dependent 2-DE results revealed that the intensity of 10 protein spots changed identically upon exposure to the three xenobiotics. Among the upregulated proteins, five protein spots, which were putative dehydrogenase, dioxygenase, and hydrolase and involved in the catabolic pathway of xenobiotic degradation, were induced. Identification of these major multifunctional proteins allowed us to map the multiple catabolic pathway for phenanthrene, naphthalene, and biphenyl degradation. A part of the initial diverse catabolism was converged into the catechol degradation branch. Detection of intermediates from 2,3-dihydroxy-biphenyl degradation to pyruvate and acetyl-CoA production by LC/MS analysis showed that ring-cleavage products of PAHs entered the tricarboxylic acid cycle, and were mineralized inS. chungbukenseDJ77. These results suggest thatS. chungbukenseDJ77 completely degrades a broad range of PAHs via a multiple catabolic pathway.
authors.affiliation: School of Biological Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Soo Youn
authors.initials: SY
authors.lastname: Lee
authors.affiliation: School of Biological Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Simranjeet Singh
authors.initials: SS
authors.lastname: Sekhon
authors.affiliation: School of Biological Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Ban
authors.affiliation: School of Biological Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Ahn
authors.affiliation: College of Veterinary Medicine, Western University of Health Sciences, Pomona, CA 91766, USA.
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Ko
authors.affiliation: College of Veterinary Medicine, Western University of Health Sciences, Pomona, CA 91766, USA.
authors.forename: Lyon
authors.initials: L
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology, Shin Ansan University, Ansan 15435, Republic of Korea.
authors.forename: Sang Yong
authors.initials: SY
authors.lastname: Kim
authors.affiliation: School of Biological Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Young-Chang
authors.initials: YC
authors.lastname: Kim
authors.affiliation: School of Biological Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D042301:Sphingomonadaceae / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011084:Polycyclic Aromatic Hydrocarbons
keywords: Sphingobium chungbukense DJ77; detoxification; multiple catabolic pathway; polycyclic aromatic hydrocarbons (PAHs); two-dimensional gel electrophoresis