title: Remodeling of Leaf Cellular Glycerolipid Composition under Drought and Re-hydration Conditions in Grasses from the Lolium-Festuca Complex.
abstract: Drought tolerant plant genotypes are able to maintain stability and integrity of cellular membranes in unfavorable conditions, and to regenerate damaged membranes after stress cessation. The profiling of cellular glycerolipids during drought stress performed on model species such as Arabidopsis thaliana does not fully cover the picture of lipidome in monocots, including grasses. Herein, two closely related introgression genotypes of Lolium multiflorum (Italian ryegrass) × Festuca arundinacea (tall fescue) were used as a model for other grass species to describe lipid rearrangements during drought and re-hydration. The genotypes differed in their level of photosynthetic capacity during drought, and in their capacity for membrane regeneration after stress cessation. A total of 120 lipids, comprising the classes of monogalactosyldiacyloglycerol, digalactosyldiacyloglycerol, sulfoquinovosyldiacylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, diacylglicerol, and triacylglicerol, were analyzed. The results clearly showed that water deficit had a significant impact on lipid metabolism in studied forage grasses. It was revealed that structural and metabolic lipid species changed their abundance during drought and re-watering periods and some crucial genotype-dependent differences were also observed. The introgression genotype characterized by an ability to regenerate membranes after re-hydration demonstrated a higher accumulation level of most chloroplast and numerous extra-chloroplast membrane lipid species at the beginning of drought. Furthermore, this genotype also revealed a significant reduction in the accumulation of most chloroplast lipids after re-hydration, compared with the other introgression genotype without the capacity for membrane regeneration. The potential influence of observed lipidomic alterations on a cellular membrane stability and photosynthetic capacity, are discussed. HIGHLIGHTS A higher drought tolerance of grasses could be associated with an earlier lipidome response to a stress signal and with a membrane regeneration after stress cessation accompanied by a turnover of chloroplast lipids.
authors.affiliation: Department of Environmental Stress Biology, Institute of Plant Genetics, Polish Academy of Sciences Poznan, Poland.
authors.forename: Dawid
authors.initials: D
authors.lastname: Perlikowski
authors.affiliation: Max Planck Institute of Molecular Plant Physiology Potsdam, Germany.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Kierszniowska
authors.affiliation: Department of Biometry and Bioinformatics, Institute of Plant Genetics, Polish Academy of Sciences Poznan, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Sawikowska
authors.affiliation: Department of Biometry and Bioinformatics, Institute of Plant Genetics, Polish Academy of Sciences Poznan, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Krajewski
authors.affiliation: Department of Plant Physiology, University of Agriculture in Krakow Krakow, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Rapacz
authors.affiliation: Max Planck Institute of Molecular Plant Physiology Potsdam, Germany.
authors.forename: Änne
authors.initials: Ä
authors.lastname: Eckhardt
authors.affiliation: Department of Environmental Stress Biology, Institute of Plant Genetics, Polish Academy of Sciences Poznan, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Kosmala
keywords: Festuca arundinacea; Lolium multiflorum; cell membranes; drought tolerance; grasses; lipidome profiling