title: Identification and Characterization of Putative Integron-Like Elements of the Heavy-Metal-Hypertolerant Strains ofPseudomonasspp.
abstract: Pseudomonasstrains isolated from the heavily contaminated Lubin copper mine and Zelazny Most post-flotation waste reservoir in Poland were screened for the presence of integrons. This analysis revealed that two strains carried homologous DNA regions composed of a gene encoding a DNA_BRE_C domain-containing tyrosine recombinase (with no significant sequence similarity to other integrases of integrons) plus a three-component array of putative integron gene cassettes. The predicted gene cassettes encode three putative polypeptides with homology to (i) transmembrane proteins, (ii) GCN5 family acetyltransferases, and (iii) hypothetical proteins of unknown function (homologous proteins are encoded by the gene cassettes of several class 1 integrons). Comparative sequence analyses identified three structural variants of these novel integron-like elements within the sequenced bacterial genomes. Analysis of their distribution revealed that they are found exclusively in strains of the genusPseudomonas.
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw 02-096, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Ciok
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw 02-096, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Adamczuk
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw 02-096, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Bartosik
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw 02-096, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Dziewit
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D032023:Integrons; D019216:Metals, Heavy / Q000378:metabolism*; D008906:Mining; D008969:Molecular Sequence Data; D010802:Phylogeny; D011044:Poland; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D045522:Recombinases / Q000235:genetics / Q000378:metabolism; D062065:Wastewater / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D019216:Metals, Heavy; D045522:Recombinases; D062065:Wastewater
keywords: Integron-like element; Lubin copper mine; Pseudomonas spp.; Zelazny Most; integron gene cassette