title: The culture of gut explants: A model to study the mucosal response.
abstract: Various experimental model designs have been used to analyze the inflammatory pathways in human gastrointestinal illnesses. Traditionally, analytical techniques and animal models are popular experimental tools to study the inflammation process of intestinal diseases. However, the comparison of results between animal and human models is difficult for the inconsistency of outcomes. Although there are different animal models for studying the intestinal diseases, none of them fully represents the physiological and environmental conditions typical of the human species. Also, there is currently a concerted effort to decrease the experimental use of animals. On the converse, experimental protocols using the culture of gut mucosa had become popular with the advent of endoscopy which allows explanting multiple fragments from the intestine. The peculiar characteristic of this model is the ability to preserve in vitro the features that we found in vivo, thus also the response to various stimuli that differs from person to person. The aim of the present paper is to provide a review of some of the possible uses of the organ intestinal mucosa culture.
authors.affiliation: Department of Medicine and Surgery, University Hospital San Giovanni di Dio e Ruggi d'Aragona, University of Salerno, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Russo
authors.affiliation: Department of Medicine and Surgery, University Hospital San Giovanni di Dio e Ruggi d'Aragona, University of Salerno, Italy.
authors.forename: Pio
authors.initials: P
authors.lastname: Zeppa
authors.affiliation: Department of Medicine and Surgery, University Hospital San Giovanni di Dio e Ruggi d'Aragona, University of Salerno, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Iovino
authors.affiliation: Department of Medicine and Surgery, University Hospital San Giovanni di Dio e Ruggi d'Aragona, University of Salerno, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Del Giorno
authors.affiliation: Department of Medicine and Surgery, University Hospital San Giovanni di Dio e Ruggi d'Aragona, University of Salerno, Italy.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Zingone
authors.affiliation: Department of Medicine and Surgery, University Hospital San Giovanni di Dio e Ruggi d'Aragona, University of Salerno, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Bucci
authors.affiliation: Department of Medicine and Surgery, University Hospital San Giovanni di Dio e Ruggi d'Aragona, University of Salerno, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Puzziello
authors.affiliation: Department of Medicine and Surgery, University Hospital San Giovanni di Dio e Ruggi d'Aragona, University of Salerno, Italy. Electronic address: cciacci@unisa.it.
authors.forename: Carolina
authors.initials: C
authors.lastname: Ciacci
mesh_terms: D000818:Animals; D035843:Biomedical Research / Q000379:methods; D003106:Colon / Q000473:pathology*; D006801:Humans; D018928:Immunity, Mucosal; D007249:Inflammation / Q000503:physiopathology*; D007413:Intestinal Mucosa / Q000502:physiology* / Q000503:physiopathology; D008954:Models, Biological; D009924:Organ Culture Techniques / Q000379:methods*
keywords: Challenge; Gut explants; In vitro culture response; Inflammation; Model of study