title: Immunogenicity and functional evaluation of iPSC-derived organs for transplantation.
abstract: Whether physiologically induced pluripotent stem cell (iPSC)-derived organs are immunogenic and can be used for transplantation is unclear. Here, we generated iPSC-derived skin, islet, and heart representing three germ layers of the body through 4n complementation and evaluated their immunogenicity and therapeutic efficacy. Upon transplantation into recipient mice, iPSC-derived skin successfully survived and repaired local tissue wounds. In diabetic mouse models, explanted iPSC-derived islets effectively produced insulin and lowered blood glucose to basal levels. iPSC-derived heart grafts maintained normal beating for more than 3 months in syngeneic recipients. Importantly, no obvious immune rejection responses against iPSC-derived organs were detected long after transplantation. Our study not only demonstrates the fundamental immunogenicity and function of iPSC derivatives, but also provides preclinical evidence to support the feasibility of using iPSC-derived skin, islet, and heart for therapeutic use.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; Graduate University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Libin
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Jiani
authors.initials: J
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Yukai
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Organ Transplantation Institute, Xiamen University, Xiamen, China.
authors.forename: Tianshu
authors.initials: T
authors.lastname: Lan
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Weixu
authors.initials: W
authors.lastname: Wang
authors.affiliation: Organ Transplantation Institute, Xiamen University, Xiamen, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Teng
authors.affiliation: Organ Transplantation Institute, Xiamen University, Xiamen, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Yan
authors.affiliation: Organ Transplantation Institute, Xiamen University, Xiamen, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: Organ Transplantation Institute, Xiamen University, Xiamen, China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Baoyang
authors.initials: B
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Organ Transplantation Institute, Xiamen University, Xiamen, China.
authors.forename: Zhongquan
authors.initials: Z
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Tongbiao
authors.initials: T
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
keywords: iPSC-derived organs; immunogenicity; transplantation