title: Electrochemical sensing of biomarker for diagnostics of bacteria-specific infections.
abstract: Pseudomonas aeruginosa is a pathogen that is prevalent in serious infections in compromised patients worldwide. A unique virulence factor of this bacterium is the redox-active molecule pyocyanin, which is a potential biomarker for the identification of P. aeruginosa infections. Here we report a direct, selective and rapid detection technique of pyocyanin.
  
  
  
  Pyocyanin was detected by amperometry at a relatively high potential where the pyocyanin signal was unaffected by background contributions.
  
  
  
  Pyocyanin was detected at concentrations down to 125 nM in a 50 µM mixture of interfering compounds with a reproducibility of r(2) = 0.999 (n = 5) within 200 s. The results document a step toward a point-of-care technique for diagnosis of P. aeruginosa infections.
authors.affiliation: Department of Micro- & Nanotechnology, Technical University of Denmark, Kgs-Lyngby, Denmark.|Department of Biotechnology & Biomedicine, Technical University of Denmark, Kgs-Lyngby, Denmark.|Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.
authors.forename: Fatima AlZahra'a
authors.initials: FA
authors.lastname: Alatraktchi
authors.affiliation: Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.|Cystisk Fibrose Klinikken & Klinisk Mikrobiologisk Afdeling - Rigshospitalet, Copenhagen, Denmark.
authors.forename: Helle Krogh
authors.initials: HK
authors.lastname: Johansen
authors.affiliation: Department of Biotechnology & Biomedicine, Technical University of Denmark, Kgs-Lyngby, Denmark.|Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.
authors.forename: Søren
authors.initials: S
authors.lastname: Molin
authors.affiliation: Department of Micro- & Nanotechnology, Technical University of Denmark, Kgs-Lyngby, Denmark.
authors.forename: Winnie Edith
authors.initials: WE
authors.lastname: Svendsen
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D019095:Point-of-Care Systems; D011552:Pseudomonas Infections / Q000175:diagnosis*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D011710:Pyocyanine / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D011710:Pyocyanine
keywords: Pseudomonas aeruginosa; amperometry; bacteria; biosensors; cystic fibrosis; detection; diagnosis; electrochemistry; electrodes; pyocyanin