title: Effect of oxidization and chitosan on the surface activity of soy protein isolate.
abstract: The objective of this research was to study the effect of oxidization of performic acid and chitosan on the structure and surface properties of soy protein isolate. As the degree of oxidization increased, the emulsifying capacity and stability of all the oxidized soy protein isolate and chitosan (SPI/CHI) systems increased substantially, which were 29.7%, 31.7%, 34.1%, 31.9% and 31.9% respectively compared. Fluorescent spectrum showed that the fluorescence intensity of SPI/CHI conjugates decreased and the higher the oxidized degree was, the lower the fluorescence intensity. Results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the location of acidic bands of SPI/CHI conjugates moved upwards and broadened. Meanwhile, the basic bands lightened or even disappeared gradually as the oxidization increased. Scanning electron microscope (SEM) showed that the particles became lager as the degree of oxidization increased. Better thermostability of the oxidized SPI/CHI systems was shown in the differential scanning calorimetry (DSC).
authors.affiliation: Faculty of Biological and Chemical Engineering, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China. Electronic address: weiwangbiochem@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Faculty of Biological and Chemical Engineering, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China.
authors.forename: Junsheng
authors.initials: J
authors.lastname: Li
authors.affiliation: Faculty of Biological and Chemical Engineering, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China. Electronic address: 274187443@qq.com.
authors.forename: Liujuan
authors.initials: L
authors.lastname: Yan
authors.affiliation: Faculty of Biological and Chemical Engineering, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China. Electronic address: 251493952@qq.com.
authors.forename: Guoxia
authors.initials: G
authors.lastname: Huang
authors.affiliation: Faculty of Biological and Chemical Engineering, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China. Electronic address: 497257411@qq.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Dong
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D010084:Oxidation-Reduction; D010316:Particle Size; D055550:Protein Stability; D030262:Soybean Proteins / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature
chemical_list: D030262:Soybean Proteins; D048271:Chitosan
keywords: Chitosan; Glycosylation; Oxidization; Performic acid; Soy protein