title: Live imaging of stem cells: answering old questions and raising new ones.
abstract: Stem cells are essential for both tissue maintenance and injury repair, but many aspects of stem cell biology remain incompletely understood. Recent advances in live imaging technology have allowed the direct visualization and tracking of a wide variety of tissue-resident stem cells in their native environments over time. Results from these studies have helped to resolve long-standing debates about stem cell regulation and function while also revealing previously unanticipated phenomena that raise new questions for future work. Here we review recent discoveries of both types, with a particular emphasis on how stem cells behave and interact with their niches during homeostasis, as well as how these behaviours change in response to wounding.
authors.affiliation: Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA.
authors.forename: Sangbum
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA; Departments of Dermatology & Cell Biology, Yale Stem Cell Center, Yale Cancer Center, Yale School of Medicine, New Haven, CT 06510, USA. Electronic address: valentina.greco@yale.edu.
authors.forename: Valentina
authors.initials: V
authors.lastname: Greco
authors.affiliation: Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA. Electronic address: katherine.cockburn@yale.edu.
authors.forename: Katie
authors.initials: K
authors.lastname: Cockburn
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000473:pathology; D049109:Cell Proliferation; D006801:Humans; D021621:Imaging, Three-Dimensional; D055153:Stem Cell Niche; D013234:Stem Cells / Q000166:cytology*; D014945:Wound Healing