title: A Practical Guide to Light Sheet Microscopy.
abstract: Light sheet fluorescence microscopy is an efficient method for imaging large volumes of biological tissue, including brains of larval zebrafish, at high spatial and fairly high temporal resolution with minimal phototoxicity.Here, we provide a practical guide for those who intend to build a light sheet microscope for fluorescence imaging in live larval zebrafish brains or other tissues.
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.|University of Chicago, Chicago, IL, USA.
authors.forename: Davis V
authors.initials: DV
authors.lastname: Bennett
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. ahrensm@janelia.hhmi.org.
authors.forename: Misha B
authors.initials: MB
authors.lastname: Ahrens
mesh_terms: D000818:Animals; D001921:Brain; D007814:Larva; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence; D015027:Zebrafish
keywords: Calcium indicators; Imaging; Light sheet; Microscopy; Systems neuroscience; Zebrafish