title: Serum-based microRNA signatures in early diagnosis and prognosis prediction of colon cancer.
abstract: Early detection of colorectal cancer is the main prerequisite for successful treatment and reduction of mortality. Circulating microRNAs were previously identified as promising diagnostic, prognostic and predictive biomarkers. The purpose of this study was to identify serum microRNAs enabling early diagnosis and prognosis prediction of colon cancer. In total, serum samples from 427 colon cancer patients and 276 healthy donors were included in three-phase biomarker study. Large-scale microRNA expression profiling was performed using Illumina small RNA sequencing. Diagnostic and prognostic potential of identified microRNAs was validated on independent training and validation sets of samples using RT-qPCR. Fifty-four microRNAs were found to be significantly deregulated in serum of colon cancer patients compared to healthy donors (P < 0.01). A diagnostic four-microRNA signature consisting of miR-23a-3p, miR-27a-3p, miR-142-5p and miR-376c-3p was established (AUC = 0.917), distinguishing colon cancer patients from healthy donors with sensitivity of 89% and specificity of 81% (AUC = 0.922). This panel of microRNAs exhibited high diagnostic performance also when analyzed separately in colon cancer patients in early stages of the disease (T1-4N0M0; AUC = 0.877). Further, a prognostic panel based on the expression of miR-23a-3p and miR-376c-3p independent of TNM stage was established (HR 2.30; 95% CI 1.44-3.66; P < 0.0004). In summary, highly sensitive signatures of circulating microRNAs enabling non-invasive early detection and prognosis prediction of colon cancer were identified.
authors.affiliation: Centre for Molecular Medicine, Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.|Department of Comprehensive Cancer Care, Faculty of Medicine, Masaryk Memorial Cancer Institute, Masaryk University, Zluty kopec 7, 656 53 Brno, Czech Republic and.
authors.forename: Petra
authors.initials: P
authors.lastname: Vychytilova-Faltejskova
authors.affiliation: Centre for Molecular Medicine, Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Radova
authors.affiliation: Department of Comprehensive Cancer Care, Faculty of Medicine, Masaryk Memorial Cancer Institute, Masaryk University, Zluty kopec 7, 656 53 Brno, Czech Republic and.
authors.forename: Milana
authors.initials: M
authors.lastname: Sachlova
authors.affiliation: Centre for Molecular Medicine, Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Kosarova
authors.affiliation: Centre for Molecular Medicine, Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Slaba
authors.affiliation: Department of Comprehensive Cancer Care, Faculty of Medicine, Masaryk Memorial Cancer Institute, Masaryk University, Zluty kopec 7, 656 53 Brno, Czech Republic and.
authors.forename: Pavel
authors.initials: P
authors.lastname: Fabian
authors.affiliation: Centre for Molecular Medicine, Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.|Department of Surgery, Institutions Shared with the Faculty Hospital Brno, Faculty of Medicine, Masaryk University, Jihlavska 340/20, 625 00 Brno, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Grolich
authors.affiliation: Department of Surgery, Institutions Shared with the Faculty Hospital Brno, Faculty of Medicine, Masaryk University, Jihlavska 340/20, 625 00 Brno, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Prochazka
authors.affiliation: Centre for Molecular Medicine, Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.|Department of Surgery, Institutions Shared with the Faculty Hospital Brno, Faculty of Medicine, Masaryk University, Jihlavska 340/20, 625 00 Brno, Czech Republic.
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Kala
authors.affiliation: Centre for Molecular Medicine, Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.|Department of Comprehensive Cancer Care, Faculty of Medicine, Masaryk Memorial Cancer Institute, Masaryk University, Zluty kopec 7, 656 53 Brno, Czech Republic and.
authors.forename: Marek
authors.initials: M
authors.lastname: Svoboda
authors.affiliation: Department of Comprehensive Cancer Care, Faculty of Medicine, Masaryk Memorial Cancer Institute, Masaryk University, Zluty kopec 7, 656 53 Brno, Czech Republic and.
authors.forename: Igor
authors.initials: I
authors.lastname: Kiss
authors.affiliation: Department of Comprehensive Cancer Care, Faculty of Medicine, Masaryk Memorial Cancer Institute, Masaryk University, Zluty kopec 7, 656 53 Brno, Czech Republic and.
authors.forename: Rostislav
authors.initials: R
authors.lastname: Vyzula
authors.affiliation: Centre for Molecular Medicine, Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.|Department of Comprehensive Cancer Care, Faculty of Medicine, Masaryk Memorial Cancer Institute, Masaryk University, Zluty kopec 7, 656 53 Brno, Czech Republic and.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Slaby
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D003110:Colonic Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology; D055088:Early Detection of Cancer; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; C555159:MIRN142 microRNA, human; C552430:MIRN23a microRNA, human; C531388:MIRN27 microRNA, human; C573498:MIRN376C microRNA, human; D035683:MicroRNAs