title: Cytomolecular discrimination of the Amchromosomes of Triticum monococcum and the A chromosomes of Triticum aestivum using microsatellite DNA repeats.
abstract: The cytomolecular discrimination of the Am- and A-genome chromosomes facilitates the selection of wheat-Triticum monococcum introgression lines. Fluorescence in situ hybridisation (FISH) with the commonly used DNA probes Afa family, 18S rDNA and pSc119.2 showed that the more complex hybridisation pattern obtained in T. monococcum relative to bread wheat made it possible to differentiate the Amand A chromosomes within homoeologous groups 1, 4 and 5. In order to provide additional chromosomal landmarks to discriminate the Amand A chromosomes, the microsatellite repeats (GAA)n, (CAG)n, (CAC)n, (AAC)n, (AGG)nand (ACT)nwere tested as FISH probes. These showed that T. monococcum chromosomes have fewer, generally weaker, simple sequence repeat (SSR) signals than the A-genome chromosomes of hexaploid wheat. A differential hybridisation pattern was observed on 6Amand 6A chromosomes with all the SSR probes tested except for the (ACT)nprobe. The 2Amand 2A chromosomes were differentiated by the signals given by the (GAA)n, (CAG)nand (AAC)nrepeats, while only (GAA)ndiscriminated the chromosomes 3Amand 3A. Chromosomes 7Amand 7A could be differentiated by the lack of (GAA)nand (AGG)nsignals on 7A. As potential landmarks for identifying the Amchromosomes, SSR repeats will facilitate the introgression of T. monococcum chromatin into wheat.
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, P.O. Box 19, 2462, Martonvásár, Hungary.
authors.forename: Mária
authors.initials: M
authors.lastname: Megyeri
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, P.O. Box 19, 2462, Martonvásár, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Mikó
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, P.O. Box 19, 2462, Martonvásár, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Farkas
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, P.O. Box 19, 2462, Martonvásár, Hungary.
authors.forename: Márta
authors.initials: M
authors.lastname: Molnár-Láng
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, P.O. Box 19, 2462, Martonvásár, Hungary. molnar.istvan@agrar.mta.hu.
authors.forename: István
authors.identifier: http://orcid.org/0000-0002-7167-9319
authors.initials: I
authors.lastname: Molnár
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D015342:DNA Probes; D018744:DNA, Plant / Q000235:genetics; D018745:Genome, Plant; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats; D014908:Triticum / Q000145:classification / Q000235:genetics*
chemical_list: D015342:DNA Probes; D018744:DNA, Plant
keywords: FISH; Karyotypic analysis; Microsatellite repeats; Triticum monococcum