title: Effects of Black Hoof Medicinal Mushroom, Phellinus linteus (Agaricomycetes), Polysaccharide Extract in Streptozotocin-Induced Diabetic Rats.
abstract: In this article we report the healing effects of a Phellinus linteus fruiting body hot water extract (PLE) in streptozotocin (STZ)-induced diabetic rats. PLE was given before and after STZ. The preprotective, protective, and postprotective effects of PLE on STZ-induced oxidative stress were studied using biochemical (caspase 3 activity, cytosolic-to-lysosomal ratio of cathepsin B and L, DNA fragmentation levels), ordinary histological and immuno-histochemical investigation parameters. Following oral administration of PLE after STZ application, the serum glucose concentration significantly decreased up to 41.13% compared with the control group (P < 0.05). The hypoglycemic potential of the PLE was further supported by an increase of insulin secretion in the islets of Langerhans. In addition, the number of cells in Langerhans islets increased by 45.89% when PLE was given after STZ application. On the other hand, the use of PLE before oxidative stress could not prevent the onset of diabetes. This is, to our knowledge, the first study of the effect of application time of orally administered Ph. Linteus hot water extract on STZ-induced diabetes.
authors.affiliation: Eskisehir Osmangazi University, Faculty of Science and Arts, Department of Biology, Eskisehir, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Yamac
authors.affiliation: Emeritus, Anadolu University, Science Faculty, Department of Biology, Eskisehir, Turkey.
authors.forename: Melih
authors.initials: M
authors.lastname: Zeytinoglu
authors.affiliation: Eskisehir Osmangazi University, Science and Arts Faculty, Department of Biology, Eskisehir, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Senturk
authors.affiliation: Eskisehir Osmangazi University, Graduate School of Medical Sciences, Eskisehir, Turkey.
authors.forename: Kazim
authors.initials: K
authors.lastname: Kartkaya
authors.affiliation: Eskisehir Osmangazi University, Medicine Faculty, Department of Biochemistry, Eskisehir, Turkey.
authors.forename: Gungor
authors.initials: G
authors.lastname: Kanbak
authors.affiliation: Artvin Coruh University, Faculty of Health Sciences, Department of Occupational Health and Safety, Artvin, Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Bayramoglu
authors.affiliation: Eskisehir Osmangazi University, Graduate School of Medical Sciences, Eskisehir, Turkey.
authors.forename: Aysegul
authors.initials: A
authors.lastname: Oglakci
authors.affiliation: Wageningen University and Research Centre, Plant Research International, Wageningen, the Netherlands.
authors.forename: Leo J L D
authors.initials: LJ
authors.lastname: Van Griensven
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy*; D004195:Disease Models, Animal; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects; D008297:Male; D000084682:Phellinus; D010936:Plant Extracts / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000009:adverse effects
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; C526145:Phellinus linteus extract; D010936:Plant Extracts; D011134:Polysaccharides; D013311:Streptozocin