title: Modelling chronotaxicity of cellular energy metabolism to facilitate the identification of altered metabolic states.
abstract: Altered cellular energy metabolism is a hallmark of many diseases, one notable example being cancer. Here, we focus on the identification of the transition from healthy to abnormal metabolic states. To do this, we study the dynamics of energy production in a cell. Due to the thermodynamic openness of a living cell, the inability to instantaneously match fluctuating supply and demand in energy metabolism results in nonautonomous time-varying oscillatory dynamics. However, such oscillatory dynamics is often neglected and treated as stochastic. Based on experimental evidence of metabolic oscillations, we show that changes in metabolic state can be described robustly by alterations in the chronotaxicity of the corresponding metabolic oscillations, i.e. the ability of an oscillator to resist external perturbations. We also present a method for the identification of chronotaxicity, applicable to general oscillatory signals and, importantly, apply this to real experimental data. Evidence of chronotaxicity was found in glycolytic oscillations in real yeast cells, verifying that chronotaxicity could be used to study transitions between metabolic states.
authors.affiliation: Department of Physics, Lancaster University, Lancaster, LA1 4YB, UK.
authors.forename: Gemma
authors.initials: G
authors.lastname: Lancaster
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
authors.forename: Yevhen F
authors.initials: YF
authors.lastname: Suprunenko
authors.affiliation: Randall Division of Cell &Molecular Biophysics, King's College London, London, WC2R 2LS, UK.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Jenkins
authors.affiliation: Department of Physics, Lancaster University, Lancaster, LA1 4YB, UK.
authors.forename: Aneta
authors.initials: A
authors.lastname: Stefanovska
mesh_terms: D001683:Biological Clocks; D004734:Energy Metabolism; D006019:Glycolysis; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013816:Thermodynamics; D013997:Time Factors