title: Toll-like receptor 7 involves the injury in acute kidney ischemia/reperfusion of STZ-induced diabetic rats.
abstract: OBJECTIVE
  To determine whether Toll-like receptor 7 (TLR7) is the potential targets of prevention or progression in the renal ischemia/reperfusion (I/R) injury of STZ-induced diabetic rats.
  
  
  METHODS
  Thirty six Sprague-Dawley rats were randomly arranged to the nondiabetic (ND) or diabetic group (DM), with each group further divided into sham (no I/R injury), I/R (ischemia-reperfusion) and CD (given by Chloroquine) group. Preoperatively, Chloroquine (40 mg/kg, intraperitoneal injection.) was administrated 6 days for treatment group. I/R animals were subjected to 25 min of bilateral renal ischemia. Renal function, histology, apoptosis, cytokines, expression of TLR7, MyD88 and NF-κB were detected.
  
  
  RESULTS
  The serum levels of blood urea nitrogen, creatinine, IL-6 and TNF-α, apoptotic tubular epithelial cells, expression of TLR7, MyD88 and NF-κB were significantly increased in DM+I/R group, compared with ND+I/R group (p<0.05). All these changes were further improved by TLR7 inhibition Chloroquine except Paller scores (p<0.05).
  
  
  CONCLUSIONS
  Toll-like receptor 7 inhibition attenuates the acute renal ischemia/reperfusion injury of STZ-induced diabetic in SD rats.
authors.affiliation: Master, Department of Anesthesia, Renmin Hospital, Wuhan University, China. Conception and design of the study, acquisition and interpretation of data, manuscript writing.
authors.forename: Huang
authors.initials: H
authors.lastname: Yayi
authors.affiliation: Master, Department of Anesthesia, Renmin Hospital, Wuhan University, China. Acquisition of data, critical revision.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yeda
authors.affiliation: PhD, Department of Anesthesia, Renmin Hospital, Wuhan University, China. Acquisition of data.
authors.forename: Wang
authors.initials: W
authors.lastname: Huaxin
authors.affiliation: PhD, Department of Anesthesia, Renmin Hospital, Wuhan University, China. Acquisition of data.
authors.forename: Wu
authors.initials: W
authors.lastname: Yang
authors.affiliation: PhD, Department of Anesthesia, Renmin Hospital, Wuhan University, China. Acquisition of data.
authors.forename: Sun
authors.initials: S
authors.lastname: Qian
authors.affiliation: PhD, Full Professor, Department of Anesthesia, Renmin Hospital, Wuhan University, China. Design and supervised all phases of the study, critical revision.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhongyuan
mesh_terms: D058186:Acute Kidney Injury / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism*; D004195:Disease Models, Animal; D020287:In Situ Nick-End Labeling / Q000379:methods; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008297:Male; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications / Q000378:metabolism*; D051199:Toll-Like Receptor 7 / Q000097:blood / Q000378:metabolism*
chemical_list: C507781:Myd88 protein, rat; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D051199:Toll-Like Receptor 7