title: From Early Embryonic to Adult Stage: Comparative Study of Action Potentials of Native and Pluripotent Stem Cell-Derived Cardiomyocytes.
abstract: Cardiomyocytes (CMs) derived from induced pluripotent stem cells (iPS-CMs) are promising candidates for cell therapy, drug screening, and developmental studies. It is known that iPS-CMs possess immature electrophysiological properties, but an exact characterization of their developmental stage and subtype differentiation is hampered by a lack of knowledge of electrophysiological properties of native CMs from different developmental stages and origins within the heart. Thus, we sought to systematically investigate action potential (AP) properties of native murine CMs and to establish a database that allows classification of stem cell-derived CMs. Hearts from 129S2PasCrl mice were harvested at days 9-10, 12-14, and 16-18 postcoitum, as well as 1 day, 3-4 days, 1-2 weeks, 3-4 weeks, and 6 weeks postpartum. AP recordings in left and right atria and at apical, medial, and basal left and right ventricles were performed with sharp glass microelectrodes. Measurements revealed significant changes in AP morphology during pre- and postnatal murine development and significant differences between atria and ventricles, enabling a classification of developmental stage and subtype differentiation of stem cell-derived CMs based on their AP properties. For iPS-CMs derived from cell line TiB7.4, a typical ventricular phenotype was demonstrated at later developmental stages, while there were electrophysiological differences from atrial as well as ventricular native CMs at earlier stages. This finding supports that iPS-CMs can develop AP properties similar to native CMs, but points to differences in the maturation process between iPS-CMs and native CMs, which may be explained by dissimilar conditions during in vitro differentiation and in vivo development.
authors.affiliation: 1 Department of Internal Medicine III, University of Cologne , Cologne, Germany .|2 Institute of Neurophysiology, University of Cologne , Cologne, Germany .
authors.forename: Gabriel
authors.initials: G
authors.lastname: Peinkofer
authors.affiliation: 2 Institute of Neurophysiology, University of Cologne , Cologne, Germany .
authors.forename: Karsten
authors.initials: K
authors.lastname: Burkert
authors.affiliation: 1 Department of Internal Medicine III, University of Cologne , Cologne, Germany .
authors.forename: Katja
authors.initials: K
authors.lastname: Urban
authors.affiliation: 1 Department of Internal Medicine III, University of Cologne , Cologne, Germany .
authors.forename: Benjamin
authors.initials: B
authors.lastname: Krausgrill
authors.affiliation: 2 Institute of Neurophysiology, University of Cologne , Cologne, Germany .
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hescheler
authors.affiliation: 2 Institute of Neurophysiology, University of Cologne , Cologne, Germany .
authors.forename: Tomo
authors.initials: T
authors.lastname: Saric
authors.affiliation: 1 Department of Internal Medicine III, University of Cologne , Cologne, Germany .
authors.forename: Marcel
authors.initials: M
authors.lastname: Halbach
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000375:Aging / Q000502:physiology*; D000818:Animals; D016275:Atrial Function / Q000502:physiology; D002454:Cell Differentiation; D004622:Embryo, Mammalian / Q000502:physiology; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000166:cytology*; D016276:Ventricular Function / Q000502:physiology