title: Cross-Reactivity between Schistosoma mansoni Antigens and the Latex Allergen Hev b 7: Putative Implication of Cross-Reactive Carbohydrate Determinants (CCDs).
abstract: IgG antibodies produced by rabbits immunized against S. mansoni antigens cross-reacted with aqueous soluble constituents of a variety of allergens. The antibody cross-reactivity was largely sensitive to degradation by treatment of the target antigens with sodium meta-periodate, suggesting the cross-reactivity was due to carbohydrate determinants that were common to both the schistosome and the allergens (CCDs). The reaction between the rabbit antibodies and a 43 kDa molecule in a rubber latex extract was analysed further: tandem mass spectrometry identified the latex molecule as allergen Hev b 7. Rabbit anti-schistosome IgG antibodies purified by acid-elution from solid-phase latex Hev b 7 reacted with the S. mansoni egg antigens IPSE/alpha-1 and kappa-5 and cercarial antigens SPO-1 and a fatty acid-binding protein. Moreover, purified anti-S. mansoni egg, latex cross-reactive antibodies reacted with antigenic constituents of some fruits, a result of potential relevance to the latex-fruit syndrome of allergic reactions. We propose that IgG anti-schistosome antibodies that cross-react with allergens may be able to block IgE-induced allergic reactions and thus provide a possible explanation for the hygiene hypothesis.
authors.affiliation: School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Doenhoff
authors.affiliation: School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.|Department of Medical Parasitology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
authors.forename: Marwa
authors.initials: M
authors.lastname: El-Faham
authors.affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Nottingham, LE12 5RD, United Kingdom.
authors.forename: Susan
authors.initials: S
authors.lastname: Liddell
authors.affiliation: School of Biological Sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, United Kingdom.
authors.forename: Heidi R
authors.initials: HR
authors.lastname: Fuller
authors.affiliation: School of Biological Sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, United Kingdom.
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Stanley
authors.affiliation: Research Center Borstel, Priority Area Asthma and Allergy, Experimental Pneumology, Parkallee 22, D-23845, Borstel, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Schramm
authors.affiliation: School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.|Department of Animal and Environmental Biology, Faculty of Life Sciences, University of Benin, Benin City, Edo State, Nigeria.
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Igetei
mesh_terms: D000818:Animals; D052179:Antigens, Plant / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D002241:Carbohydrates / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D010940:Plant Proteins / Q000276:immunology*; D011817:Rabbits; D012550:Schistosoma mansoni / Q000276:immunology*; D053719:Tandem Mass Spectrometry
chemical_list: D052179:Antigens, Plant; D000953:Antigens, Protozoan; D002241:Carbohydrates; D010940:Plant Proteins; C113002:allergen Hev b 7