title: Affinity Monolith-Integrated Microchips for Protein Purification and Concentration.
abstract: Affinity chromatography is a valuable method to purify and concentrate minute amount of proteins. Monoliths with epoxy groups for affinity immobilization were prepared by direct in-situ photopolymerization of glycidyl methacrylate and ethylene glycol dimethacrylate in porogenic solvents consisting of 1-dodecanol and cyclohexanol. By integrating affinity monoliths onto a microfluidic system, targeted biomolecules can be captured and retained on affinity column, while other biomolecules having no specific interactions toward the immobilized ligands flow through the microchannel. Therefore, proteins which remain on the affinity column are purified and concentrated, and then eluted by appropriate solutions and finally, separated by microchip capillary electrophoresis. This integrated microfluidic device has been applied to the purification and separation of specific proteins (FITC-labeled human serum albumin and IgG) in a mixture.
authors.affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, 2 West Wenhua Road, Shandong, 264209, China. clgao@hitwh.edu.cn.
authors.forename: Changlu
authors.initials: C
authors.lastname: Gao
authors.affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, 2 West Wenhua Road, Shandong, 264209, China. xsunwendy@163.com.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Sun
authors.affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, 2 West Wenhua Road, Shandong, 264209, China.
authors.forename: Huaixin
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, 2 West Wenhua Road, Shandong, 264209, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qiao
authors.affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, 2 West Wenhua Road, Shandong, 264209, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Hu
mesh_terms: D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004852:Epoxy Compounds / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000302:isolation & purification; D056656:Lab-On-A-Chip Devices; D008689:Methacrylates / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D000075462:Serum Albumin, Human / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D004852:Epoxy Compounds; D007074:Immunoglobulin G; D008689:Methacrylates; D011506:Proteins; C004919:ethylene dimethacrylate; D016650:Fluorescein-5-isothiocyanate; C007870:glycidyl methacrylate; D000075462:Serum Albumin, Human
keywords: Affinity; Concentration; Microchip; Monolith; Purification