title: Calcium Imaging of Neuronal Activity in Free-Swimming Larval Zebrafish.
abstract: Visualization of neuronal activity during animal behavior is a critical step in understanding how the brain generates behavior. In the model vertebrate zebrafish, imaging of the brain has been done mostly by using immobilized fish. Here, we describe a novel method to image neuronal activity of the larval zebrafish brain during prey capture behavior. We expressed a genetically encoded fluorescent calcium indicator, GCaMP, in the optic tectum of the midbrain using the Gal4-UAS system. Tectal activity was then imaged in unrestrained larvae during prey perception. Since larval zebrafish swim only intermittently, detection of the neuronal activity is possible between swimming bouts. Our method makes functional brain imaging under natural behavioral conditions feasible and will greatly benefit the study of neuronal activities that evoke animal behaviors.
authors.affiliation: Division of Molecular and Developmental Biology, Department of Genetics, National Institute of Genetics, SOKENDAI (The Graduate University for Advanced Studies), 1111 Yata, Mishima, Shizuoka, 411-8540, Japan. akimuto@nig.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Muto
authors.affiliation: Division of Molecular and Developmental Biology, Department of Genetics, National Institute of Genetics, SOKENDAI (The Graduate University for Advanced Studies), 1111 Yata, Mishima, Shizuoka, 411-8540, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001921:Brain / Q000502:physiology; D007814:Larva / Q000378:metabolism / Q000502:physiology*; D013550:Swimming / Q000502:physiology*; D014796:Visual Perception / Q000502:physiology; D015027:Zebrafish / Q000378:metabolism / Q000502:physiology
keywords: Calcium imaging; GCaMP; Gal4-UAS system; Paramecium; Prey capture; Tectum; Vision