title: Phage-Directed Synthesis of Photoluminescent Zinc Oxide Nanoparticles under Benign Conditions.
abstract: Biological systems, especially bacteriophages and peptides, are an attractive green alternative to other known methods of nanoparticle synthesis. In this work, for the first time, bacteriophages were employed to synthesize a specific peptide, capable of producing nanoparticles (NPs). Derivatives of M13 bacteriophage exposing a ZnO-binding peptide (TMGANLGLKWPV) on either pIII or pVIII phage coat protein were constructed and used as a biotemplate. The exposition of the ZnO-binding peptide, synthesized by phages during their propagation in bacteria, on M13 virions provided a groundwork for growing ZnO nanostructures. Depending on the recombinant phage type used (M13-pIII-ZnO or M13-pVIII-ZnO), well separated ZnO NPs or complex 3D structures of ZnO NPs of ca. 20-40 nm were synthesized at room temperature. The synthesized ZnO nanoparticles served as a luminescent material that emitted light near the short wavelength end of the visible region (at ca. 400 nm). The next very low intensity emission band at 530 nm demonstrated that the ZnO material obtained is characterized by a low concentration of surface defects.
authors.affiliation: Solid State Physics Department, Faculty of Applied Physics and Mathematics, Gdansk University of Technology , Narutowicza 11/12, 80-233 Gdansk, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Żelechowska
authors.affiliation: Department of Molecular Biology, University of Gdansk , Wita Stwosza 59, 80-308 Gdansk, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Karczewska-Golec
authors.affiliation: Solid State Physics Department, Faculty of Applied Physics and Mathematics, Gdansk University of Technology , Narutowicza 11/12, 80-233 Gdansk, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Karczewski
authors.affiliation: Department of Molecular Biology, University of Gdansk , Wita Stwosza 59, 80-308 Gdansk, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Łoś
authors.affiliation: Faculty of Chemistry, University of Gdansk , Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Andrzej M
authors.initials: AM
authors.lastname: Kłonkowski
authors.affiliation: Department of Molecular Biology, University of Gdansk , Wita Stwosza 59, 80-308 Gdansk, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Węgrzyn
authors.affiliation: Laboratory of Molecular Biology (affiliated with the University of Gdansk), Institute of Biochemistry and Biophysics, Polish Academy of Sciences , Wita Stwosza 59, 80-308 Gdansk, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Golec
mesh_terms: D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000378:metabolism*; D049408:Luminescent Agents / Q000737:chemistry* / Q000378:metabolism*; D053758:Nanoparticles; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D014771:Virion / Q000378:metabolism; D015034:Zinc Oxide / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D049408:Luminescent Agents; D010455:Peptides; D015034:Zinc Oxide