title: Design, synthesis, and negative inotropic evaluation of 4-phenyl-1H-1,2,4-triazol-5(4H)-one derivatives containing triazole or piperazine moieties.
abstract: In this study, four novel series of 4-phenyl-1H-1,2,4-triazol-5(4H)-one derivatives containing triazole or piperazine moieties were designed, synthesized, and evaluated for negative inotropic activity by measuring the left atrium stroke volume in isolated rabbit heart preparations. Almost all of the compounds showed an ability to moderate the cardiac workload by decreasing the heart rate and contractility. Among them, 7h was found to be the most potent with a change in stroke volume of -48.22 ± 0.36% at a concentration of 3 × 10-5mol/L (metoprolol: -9.74 ± 0.14%). The cytotoxicity of these compounds was evaluated using the human cervical cancer cell line HeLa, the liver cancer cell line Hep3B, and the human normal hepatic cell line LO2. A preliminary study of the mechanism of action for the compound 7h on the regulation of atrial dynamics with ATP-sensitive K+channel and L-type Ca2+channel blockers glibenclamide and nifedipine was performed in the isolated perfused beating rabbit atria.
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University College of Pharmacy, Yanji, China.
authors.forename: Zhi-Yu
authors.initials: ZY
authors.lastname: Wei
authors.affiliation: Department of Orthopedics, Yanbian University Hospital, Yanji, China.
authors.forename: Bai-Ri
authors.initials: BR
authors.lastname: Cui
authors.affiliation: Yanbian University College of Medicine, Yanji, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Cui
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University College of Pharmacy, Yanji, China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Wu
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University College of Pharmacy, Yanji, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Yanbian University College of Medicine, Yanji, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Liu
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University College of Pharmacy, Yanji, China.
authors.forename: Hu-Ri
authors.initials: HR
authors.lastname: Piao
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D066298:In Vitro Techniques; D010879:Piperazines / Q000737:chemistry; D011817:Rabbits; D014230:Triazoles / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D002316:Cardiotonic Agents; D010879:Piperazines; D014230:Triazoles
keywords: [1,2,4]triazol-5(4H)-one; negative inotropic activity; piperazine; stroke volume; triazole