title: Anti-aging properties of Ribes fasciculatum in Caenorhabditis elegans.
abstract: The present study investigated the effects and underlying mechanism of ethylacetate fraction of Ribes fasciculatum (ERF) on the lifespan and stress tolerance using a Caenorhabditis elegans model. The longevity activity of ERF was determined by lifespan assay under normal culture condition. The survival rate of nematodes under various stress conditions was assessed to validate the effects of ERF on the stress tolerance. To determine the antioxidant potential of ERF, the superoxide dismutase (SOD) activities and intracellular reactive oxygen species (ROS) levels were investigated. The ERF-mediated change in SOD-3 expression was examined using GFP-expressing transgenic strain. The effects of ERF on the aging-related factors were investigated by reproduction assay and pharyngeal pumping assay. The intestinal lipofuscin levels of aged nematodes were also measured. The mechanistic studies were performed using selected mutant strains. Our results indicated that ERF showed potent lifespan extension effects on the wild-type nematode under both normal and various stress conditions. The ERF treatment also enhanced the activity and expression of superoxide dismutase (SOD) and attenuated the intracellular ROS levels. Moreover, ERF-fed nematodes showed decreased lipofuscin accumulation, indicating ERF might affect age-associated changes in C. elegans. The results of mechanistic studies indicated that there was no significant lifespan extension in ERF-treated daf-2, age-1, sir-2.1, and daf-16 null mutants, suggesting that they were involved in ERF-mediated lifespan regulation. In conclusion, R. fasciculatum confers increased longevity and stress resistance in C. elegans via SIR-2.1-mediated DAF-16 activation, dependent on the insulin/IGF signaling pathway.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Woosuk University, Jeonbuk 55338, Republic of Korea.
authors.forename: Hoon
authors.initials: H
authors.lastname: Jeon
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Woosuk University, Jeonbuk 55338, Republic of Korea. Electronic address: cha@woosuk.ac.kr.
authors.forename: Dong Seok
authors.initials: DS
authors.lastname: Cha
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008136:Longevity / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D031965:Ribes / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D029742:Caenorhabditis elegans Proteins; D010936:Plant Extracts; D017382:Reactive Oxygen Species
keywords: Aging; Caenorhabditis elegans; Lifespan; Ribes fasciculatum; Stress tolerance