title: Correlation between Ultrasound-guided Diffuse Optical Tomography and Hypoxia-inducible Factor-1Α of Breast Cancer.
abstract: Objective  To investigate the correlation between ultrasound-guided diffuse optical tomography (US-DOT) and hypoxia-inducible factor-1Α (HIF-1Α) of breast cancer.  Methods Totally 69 patients with pathologically confirmed breast cancer underwent preoperative conventional breast ultrasonography examinations and US-DOT at Peking Union Medical College Hospital From October 2007 to February 2010 were enrolled in this study.After surgery,immunohistochemical staining of HIF-1Α and CD34 were performed,and the differences of total hemoglobin concentration (THC) and microvessel density (MVD) between HIF-1Α positive and negative groups were analyzed.  Results  HIF-1Α was positive in 12 cases (17.4%) and negative in 57 cases (82.6%). The average THC and MVD of HIF-1Α-positive cases were (274.763±77.661) Μmol/L and (33.8±10.8)/0.2 mm(2) respectively. The average THC and MVD of HIF-1Α-negative cases were (228.059±65.760)Μmol/L and (28.4±7.4)/0.2 mm(2). MVD(t=2.049,P=0.04) and THC(t=2.167,P=0.034) of HIF-1Α-positive group were significantly higher than those of HIF-1Α-negative group. Conclusions HIF-1Α can promote tumor angiogenesis and thus increase the blood supply and THC. As an indicator of tumor blood supply,THC can indirectly reflect the angiogenic activity of breast cancer.
authors.affiliation: 1Department of Ultrasound,PUMC Hospital,CAMS and PUMC,Beijing 100730,China;
authors.forename: Si-hua
authors.lastname: Niu
authors.affiliation: 1Department of Ultrasound,PUMC Hospital,CAMS and PUMC,Beijing 100730,China;
authors.forename: Qing-li
authors.lastname: Zhu
authors.affiliation: 1Department of Ultrasound,PUMC Hospital,CAMS and PUMC,Beijing 100730,China;
authors.forename: Yu-xin
authors.lastname: Jiang
authors.affiliation: 2Department of Ultrasound,Peking University People’s Hospital,Beijing,100044,China;
authors.forename: Jia-an
authors.lastname: Zhu
authors.affiliation: 1Department of Ultrasound,PUMC Hospital,CAMS and PUMC,Beijing 100730,China;
authors.forename: Meng-su
authors.lastname: Xiao
authors.affiliation: 1Department of Ultrasound,PUMC Hospital,CAMS and PUMC,Beijing 100730,China;
authors.forename: Shan-shan
authors.lastname: You
authors.affiliation: 3Department of Pathology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
authors.forename: Wei-xun
authors.lastname: Zhou
authors.affiliation: 3Department of Pathology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
authors.forename: Yu
authors.lastname: Xiao
mesh_terms: D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism*; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D009389:Neovascularization, Pathologic; D041622:Tomography, Optical; D016217:Ultrasonography, Mammary
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit