title: Detection of glucose based on the peroxidase-like activity of reduced state carbon dots.
abstract: It was found that reduced state carbon dots (r-CDs) possessed intrinsic peroxidase-like activity, and could catalytically oxidize 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 to produce a color reaction. The effects of temperature, pH, incubating time and the concentration of H2O2 and TMB on catalytic activity of r-CDs were investigated. Finally we calculated the kinetic constant was ca. 0.00729min(-1) and applied r-CDs to glucose sensing by coupling glucose oxidase. As low as 2µM H2O2 could be detected with a linear range from 0.010 to 0.40mM via this method. This study offered a simple, sensitive, and high selectivity method for glucose determination even in serum.
authors.affiliation: Key Laboratory on Luminescent and Real-Time Analytic Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Yijuan
authors.initials: Y
authors.lastname: Long
authors.affiliation: Key Laboratory on Luminescent and Real-Time Analytic Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory on Luminescent and Real-Time Analytic Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Dongjun
authors.initials: D
authors.lastname: Shen
authors.affiliation: Key Laboratory on Luminescent and Real-Time Analytic Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: zhenghz@swu.edu.cn.
authors.forename: Huzhi
authors.initials: H
authors.lastname: Zheng
mesh_terms: D001560:Benzidines / Q000737:chemistry; D002244:Carbon / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003124:Colorimetry; D005947:Glucose / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D009195:Peroxidase / Q000378:metabolism*; D013696:Temperature
chemical_list: D001560:Benzidines; D002244:Carbon; D006861:Hydrogen Peroxide; D009195:Peroxidase; D005947:Glucose
keywords: Chromogenic reaction; Glucose; Peroxidase-like activity; Reduced carbon dots