title: Soft Tissue Augmentation with Autologous Platelet Gel and β-TCP: A Histologic and Histometric Study in Mice.
abstract: Background. Facial aging is a dynamic process involving both soft tissue and bony structures. Skin atrophy, with loss of tone, elasticity, and distribution of facial fat, coupled with gravity and muscle activity, leads to wrinkling and folds. Purpose. The aim of the study was to evaluate microporous tricalcium phosphate (β-TCP) and autologous platelet gel (APG) mix in mice for oral and maxillofacial soft tissue augmentation. The hypothesis was that β-TCP added with APG was able to increase the biostimulating effect on fibroblasts and quicken resorption. Materials and Methods. Ten female, 6-8-week-old black-haired mice were selected. β-TCP/APG gel was injected into one cheek; the other was used as control. The animals were sacrificed at 8 weeks and histologically evaluated. Results. The new fibroblast was intensively stained with acid fuchsin and presented in contact with β-TCP. At higher magnification, actively secreting fibroblasts were observed at the periphery of β-TCP with a well differentiated fibroblast cell line and blood vessels. Acid fuchsin stained cutaneous structures in pink: no epidermal/dermal alterations or pathological inflammatory infiltrates were detected. The margins of β-TCP granules were clear and not diffused near tissues. Conclusion. APG with β-TCP preserves skin morphology, without immune response, with an excellent tolerability and is a promising scaffold for cells and biomaterial for soft tissue augmentation.
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences and CeSI-MeT, University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Antonio
authors.identifier: 0000-0001-5528-0853
authors.initials: A
authors.lastname: Scarano
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Ceccarelli
authors.affiliation: Department of Surgery Sciences, University of Rome "La Sapienza", Piazzale Aldo Moro 5, 00185 Roma, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Marchetti
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Piattelli
authors.affiliation: Oral Surgery Unit, Regina Margherita Pediatric Hospital and University of Eastern Piedmont, Viale Piazza d'Armi 1, 28100 Novara, Italy.
authors.forename: Carmen
authors.initials: C
authors.lastname: Mortellaro
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D000818:Animals; D002130:Calcium Phosphates / Q000008:administration & dosage*; D002454:Cell Differentiation / Q000187:drug effects; D005145:Face / Q000503:physiopathology*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D051379:Mice; D017713:Platelet Transfusion; D015595:Skin Aging / Q000187:drug effects / Q000473:pathology; D064746:Therapy, Soft Tissue; D014182:Transplantation, Autologous
chemical_list: D002130:Calcium Phosphates; C018392:tricalcium phosphate