title: Persimmon-Tannin, an α-Amylase Inhibitor, Retards Carbohydrate Absorption in Rats.
abstract: Inhibitors of carbohydrate-hydrolyzing enzymes play an important role in controlling postprandial blood glucose levels. Thus the effect of persimmon tannin on pancreatic α-amylase and intestinal α-glucosidase has been investigated. Persimmon tannin inhibits pancreatic α-amylase and intestinal α-glucosidase in a concentration-dependent manner with the 50% inhibition concentration (IC50) for amylase, maltase and sucrase being 1.7 μg/mL, 632 μg/mL and 308 μg/mL, respectively. The effect of persimmon-tannin extract on carbohydrate absorption in rats has also been investigated. Oral administration of persimmon tannin to normal rats fed cornstarch (2 g/kg body weight) significantly suppressed the increase in blood glucose levels and the area under the curve (AUC) after starch loading in a dose-dependent manner. The effective dose of persimmon tannin required to achieve 50% suppression of the rise in blood glucose level was estimated to be 300 mg/kg body weight. Administration of persimmon tannin to rats fed maltose or sucrose delayed the increase of blood glucose level and slightly suppressed AUC, but not significantly. These results suggest that persimmon tannin retards absorption of carbohydrate and reduces post-prandial hyperglycemia mainly through inhibition of α-amylase.
authors.affiliation: Division of Analytical Bio-medicine, Advanced Research Support Center, Ehime University.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tsujita
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D004040:Dietary Carbohydrates / Q000493:pharmacokinetics*; D029788:Diospyros / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D007408:Intestinal Absorption / Q000187:drug effects; D008297:Male; D008320:Maltose / Q000008:administration & dosage; D019518:Postprandial Period; D051381:Rats; D017208:Rats, Wistar; D013213:Starch / Q000008:administration & dosage / Q000493:pharmacokinetics; D013395:Sucrose / Q000008:administration & dosage; D013634:Tannins / Q000008:administration & dosage / Q000494:pharmacology*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D001786:Blood Glucose; D004040:Dietary Carbohydrates; D004791:Enzyme Inhibitors; D013634:Tannins; D013395:Sucrose; D008320:Maltose; D013213:Starch; D000516:alpha-Amylases