title: Integrated Approach To Producing High-Purity Trehalose from Maltose by the Yeast Yarrowia lipolytica Displaying Trehalose Synthase (TreS) on the Cell Surface.
abstract: An alternative strategy that integrated enzyme production, trehalose biotransformation, and bioremoval in one bioreactor was developed in this study, thus simplifying the traditional procedures used for trehalose production. The trehalose synthase gene from a thermophilic archaea, Picrophilus torridus, was first fused to the YlPir1 anchor gene and then inserted into the genome of Yarrowia lipolytica, thus yielding an engineered yeast strain. The trehalose yield reached 73% under optimal conditions. The thermal and pH stabilities of the displayed enzyme were improved compared to those of its free form purified from recombinant Escherichia coli. After biotransformation, the glucose byproduct and residual maltose were directly fermented to ethanol by a Saccharomyces cerevisiae strain. Ethanol can be separated by distillation, and high-purity trehalose can easily be obtained from the fermentation broth. The results show that this one-pot procedure is an efficient approach to the economical production of trehalose from maltose.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Innovation & Application Institute (IAI), Zhejiang Ocean University , Zhoushan 316022, China.
authors.forename: Hengwei
authors.initials: H
authors.lastname: Wang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Li
authors.forename: Huiling
authors.initials: H
authors.lastname: Cheng
authors.forename: Dawen
authors.initials: D
authors.lastname: Liu
authors.forename: Hairong
authors.initials: H
authors.lastname: Cheng
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008320:Maltose / Q000378:metabolism*; D019603:Thermoplasmales / Q000201:enzymology* / Q000235:genetics; D014199:Trehalose / Q000378:metabolism*; D025062:Yarrowia / Q000235:genetics / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D000431:Ethanol; D008320:Maltose; D014199:Trehalose; D005964:Glucosyltransferases; C099692:trehalose synthase; D005947:Glucose
keywords: Picrophilus torridus; Yarrowia lipolytica; cell surface display; ethanol; trehalose; trehalose synthase