title: Serial femtosecond crystallography datasets from G protein-coupled receptors.
abstract: We describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors are: the human serotonin receptor 2B in complex with an agonist ergotamine, the human δ-opioid receptor in complex with a bi-functional peptide ligand DIPP-NH2, the human smoothened receptor in complex with an antagonist cyclopamine, and finally the human angiotensin II type 1 receptor in complex with the selective antagonist ZD7155. All four datasets have been deposited, with minimal processing, in an HDF5-based file format, which can be used directly for crystallographic processing with CrystFEL or other software. We have provided processing scripts and supporting files for recent versions of CrystFEL, which can be used to validate the data.
authors.affiliation: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: White
authors.affiliation: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
authors.forename: Anton
authors.initials: A
authors.lastname: Barty
authors.affiliation: School of Molecular Sciences, and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona 85287-1604, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Departments of Chemistry and Physics &Astronomy, The Bridge Institute, University of Southern California, Los Angeles, California 90089, USA.
authors.forename: Andrii
authors.initials: A
authors.lastname: Ishchenko
authors.affiliation: Departments of Chemistry and Physics &Astronomy, The Bridge Institute, University of Southern California, Los Angeles, California 90089, USA.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK.
authors.forename: Cornelius
authors.initials: C
authors.lastname: Gati
authors.affiliation: Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Nadia A
authors.initials: NA
authors.lastname: Zatsepin
authors.affiliation: School of Molecular Sciences, and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona 85287-1604, USA.
authors.forename: Shibom
authors.initials: S
authors.lastname: Basu
authors.affiliation: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Oberthür
authors.affiliation: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Metz
authors.affiliation: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Beyerlein
authors.affiliation: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
authors.forename: Chun Hong
authors.initials: CH
authors.lastname: Yoon
authors.affiliation: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany.
authors.forename: Oleksandr M
authors.initials: OM
authors.lastname: Yefanov
authors.affiliation: Paul Scherrer Institute, Villigen CH-5232, Switzerland.
authors.forename: Daniel
authors.identifier: http://orcid.org/0000-0002-8348-6661
authors.initials: D
authors.lastname: James
authors.affiliation: iHuman Institute, ShanghaiTech University, 2F Building 6, 99 Haike Road, Pudong New District, Shanghai 201210, China.
authors.forename: Dingjie
authors.initials: D
authors.lastname: Wang
authors.affiliation: National Science Foundation BioXFEL Science and Technology Center, 700 Ellicott Street, Buffalo, New York 14203, USA.
authors.forename: Marc
authors.initials: M
authors.lastname: Messerschmidt
authors.affiliation: Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
authors.forename: Jason E
authors.initials: JE
authors.lastname: Koglin
authors.affiliation: Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Boutet
authors.affiliation: Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Uwe
authors.initials: U
authors.lastname: Weierstall
authors.affiliation: Departments of Chemistry and Physics &Astronomy, The Bridge Institute, University of Southern California, Los Angeles, California 90089, USA.
authors.forename: Vadim
authors.identifier: http://orcid.org/0000-0002-5265-3914
authors.initials: V
authors.lastname: Cherezov
mesh_terms: D003461:Crystallography; D006801:Humans; D007834:Lasers; D008055:Lipids / Q000737:chemistry; D012984:Software; D014961:X-Ray Diffraction
chemical_list: D008055:Lipids