title: Effects of Chain Length and Saturability of Fatty Acids on Phospholipids and Proteins in Plasma Membranes of Bovine Mammary Gland.
abstract: Free fatty acids (FFAs) in plasma are essential substrates for de novo synthesis of milk fat, or directly import into mammary cells. The physico-chemical properties of mammary cells membrane composition affected by FFAs with different chain lengths and saturability are unclear yet. Employing GC, FTIR and fluorescence spectroscopy, the adsorption capacity, phospholipids content, membrane proteins conformation, lipid peroxidation product, and free sulfhydryl of plasma membranes (PMs) interacted with different FFAs were determined. The mammary cells PMs at 38 and 39.5 °C showed different adsorption capacities: acetic acid (Ac) > stearic acid (SA) > β-hydroxybutyric acid (BHBA) > trans10, cis12 CLA. In the FTIR spectrum, the major adsorption peaks appeared at 2920 and 2850 cm-1for phospholipids, and at 1628 and 1560 cm-1for membrane proteins. The intensities of PMs-FFAs complexes were varied with the FFAs species and their initial concentrations. The β-sheet and turn structures of membrane proteins were transferred into random coil and α-helix after BHBA, SA and trans10, cis12 CLA treatments compared with Ac treatment. The quenching effects on the fluorescence of endogenous membrane protein, 1, 8-ANS, NBD-PE, and DHPE entrapped in PMs by LCFA were different from those of short chain FFAs. These results indicate that the adsorption of FFAs could change membrane protein conformation and polarity of head group in phospholipids. This variation of the mammary cells PMs was regulated by carbon chain length and saturability of FFAs.
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock and Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, P.O. Box 10#, 410125, Changsha, Hunan, People's Republic of China.
authors.forename: Qiongxian
authors.identifier: 0000-0003-0035-6819
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock and Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, P.O. Box 10#, 410125, Changsha, Hunan, People's Republic of China.
authors.forename: Shaoxun
authors.initials: S
authors.lastname: Tang
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock and Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, P.O. Box 10#, 410125, Changsha, Hunan, People's Republic of China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Animal Science Faculty of Agriculture, University of Benin, P.M.B.1154, Benin, Nigeria.
authors.forename: Musibau Adungbe
authors.initials: MA
authors.lastname: Bamikole
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock and Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, P.O. Box 10#, 410125, Changsha, Hunan, People's Republic of China.
authors.forename: Chuanshe
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock and Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, P.O. Box 10#, 410125, Changsha, Hunan, People's Republic of China.
authors.forename: Jinhe
authors.initials: J
authors.lastname: Kang
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock and Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, P.O. Box 10#, 410125, Changsha, Hunan, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock and Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, P.O. Box 10#, 410125, Changsha, Hunan, People's Republic of China. zltan@isa.ac.cn.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Tan
mesh_terms: D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005260:Female; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D008565:Membrane Proteins; D010743:Phospholipids
keywords: Bovine; Free fatty acids; Phospholipids; Plasma membranes; Protein conformation