title: Molecular cloning of kisspeptin receptor genes (gpr54-1 and gpr54-2) and their expression profiles in the brain of a tropical damselfish during different gonadal stages.
abstract: The kisspeptin receptor (GPR54) mediates neuroendocrine control of kisspeptin in the brain and acts as a gateway for a pulsatile release of hypothalamic gonadotropin-releasing hormone. This study aimed to clone two GPR54 genes (gpr54-1 and gpr54-2) from the brain of the sapphire devil Chrysiptera cyanea, a tropical damselfish, and to study their involvement in reproduction. The partial sequences of the sapphire devil gpr54-1 cDNA (1059bp) and gpr54-2 cDNA (1098bp) each had an open reading frame encoding a protein of 353 and 366 amino acids, respectively, both of which had structural features of a G-protein-coupled receptor. The expression of gpr54-1 mRNA was observed in the diencephalon and telencephalon, and gpr54-2 mRNA was found in the optic tectum of sapphire devil. When gpr54-1 and gpr54-2 mRNA levels were examined in the brain of sapphire devil by real-time quantitative polymerase chain reaction (qPCR), they were found to increase during late vitellogenesis and post-spawning. Treatment of fish with estradiol-17β (Ε2) resulted in an increase in gpr54-1 and gpr54-2 expression in the brain of sapphire devil. Thus, kisspeptin receptors likely mediate the activity of kisspeptin in the brain and are involved in controlling reproductive events in a tropical damselfish.
authors.affiliation: Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Imamura
authors.affiliation: Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan.
authors.forename: Sung-Pyo
authors.initials: SP
authors.lastname: Hur
authors.affiliation: Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takeuchi
authors.affiliation: Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan.
authors.forename: Selma
authors.initials: S
authors.lastname: Bouchekioua
authors.affiliation: Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan. Electronic address: takemura@sci.u-ryukyu.ac.jp.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Takemura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005399:Fishes / Q000145:classification / Q000378:metabolism*; D020869:Gene Expression Profiling; D059648:Kisspeptins / Q000378:metabolism*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D059648:Kisspeptins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface
keywords: Coral reef; Damselfish; Kisspeptin; Real-time PCR; Sapphire devil; Sex steroids; Vitellogenesis