title: CD4+/CD25(high)/FoxP3+/CD127- regulatory T cells in bronchoalveolar lavage fluid of lung cancer patients.
abstract: The aim of the study was to compare the presence of regulatory T cells (Tregs) in the local lung cancer environment versus systemic immune response based on the examination of bronchoalveolar lavage fluid (BALf) and peripheral blood (PB) from the same patient. 35 patients with lung cancer were investigated. Flow cytometry method with panel of antibodies: anti CD4/CD25/FoxP3/CD127 for Tregs identification was used. We observed significantly higher proportion of Tregs in the BALF than in PB (median 9.4 vs. 5.4%, p<0.05). The increased proportion of Tregs in patients with advanced disease and in adenocarcinoma was found. This study confirmed the usefulness of BALF analysis in evaluation of immune response in lung cancer. Detection of Tregs in the local tumour environment may have therapeutic relevance in individual indication for anti-cancer immune-therapies.
authors.affiliation: Department of Pathology, Medical University of Warsaw, Warsaw, Poland; Department of Pneumonology and Allergology, Medical University of Warsaw, Warsaw, Poland. Electronic address: osinskaiwi@gmail.com.
authors.forename: Iwona
authors.initials: I
authors.lastname: Osińska
authors.affiliation: Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Medical University of Warsaw, Warsaw, Poland. Electronic address: anna.stelmaszczyk-emmel@wum.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Stelmaszczyk-Emmel
authors.affiliation: Department of Surgery, National Institute of Tuberculosis and Lung Diseases, Warsaw, Poland. Electronic address: mpk@poczta.neostrada.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Polubiec-Kownacka
authors.affiliation: Department of Surgery, National Institute of Tuberculosis and Lung Diseases, Warsaw, Poland. Electronic address: drdariuszdziedzic@gmail.com.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Dziedzic
authors.affiliation: Department of Pneumonology and Allergology, Medical University of Warsaw, Warsaw, Poland. Electronic address: domagalakulawik@gmail.com.
authors.forename: Joanna
authors.initials: J
authors.lastname: Domagała-Kulawik
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology*; D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D015704:CD4 Antigens / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D053699:Interleukin-7 Receptor alpha Subunit / Q000378:metabolism; D008175:Lung Neoplasms / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D059016:Tumor Microenvironment
chemical_list: D015704:CD4 Antigens; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit; D053699:Interleukin-7 Receptor alpha Subunit
keywords: Bronchoalveolar lavage fluid; Flow cytometry; Lung cancer; T regulatory cells