title: D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamicum Cells Expressing D-Allulose 3-Epimerase Flavonifractor plautii.
abstract: A d-allulose 3-epimerase from Flavonifractor plautii was cloned and expressed in Escherichia coli and Corynebacterium glutamicum. The maximum activity of the enzyme purified from recombinant E. coli cells was observed at pH 7.0, 65°C, and 1 mM Co2+ with a half-life of 40 min at 65°C, Km of 162 mM, and kcat of 25280 1/s. For increased d-allulose production, recombinant C. glutamicum cells were permeabilized via combined treatments with 20 mg/L penicillin and 10% (v/v) toluene. Under optimized conditions, 10 g/L permeabilized cells produced 235 g/L d-allulose from 750 g/L d-fructose after 40 min, with a conversion rate of 31% (w/w) and volumetric productivity of 353 g/L/h, which were 1.4- and 2.1-fold higher than those obtained for nonpermeabilized cells, respectively.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, South Korea.
authors.forename: Chul-Soon
authors.initials: CS
authors.lastname: Park
authors.affiliation: Department of Food Science and Technology, Sejong University, Seoul, 05006, South Korea.
authors.forename: Taeyong
authors.initials: T
authors.lastname: Kim
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, South Korea.
authors.forename: Seung-Hye
authors.initials: SH
authors.lastname: Hong
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, South Korea.
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Shin
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, South Korea.
authors.forename: Kyoung-Rok
authors.initials: KR
authors.lastname: Kim
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, South Korea.
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D020134:Catalytic Domain; D048230:Corynebacterium glutamicum / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005632:Fructose / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010539:Permeability; D019998:Racemases and Epimerases / Q000378:metabolism*; D013696:Temperature
chemical_list: C003243:psicose; D005632:Fructose; D019998:Racemases and Epimerases