title: Association of six CpG-SNPs in the inflammation-related genes with coronary heart disease.
abstract: Chronic inflammation has been widely considered to be the major risk factor of coronary heart disease (CHD). The goal of our study was to explore the possible association with CHD for inflammation-related single nucleotide polymorphisms (SNPs) involved in cytosine-phosphate-guanine (CpG) dinucleotides. A total of 784 CHD patients and 739 non-CHD controls were recruited from Zhejiang Province, China. Using the Sequenom MassARRAY platform, we measured the genotypes of six inflammation-related CpG-SNPs, including IL1B rs16944, IL1R2 rs2071008, PLA2G7 rs9395208, FAM5C rs12732361, CD40 rs1800686, and CD36 rs2065666). Allele and genotype frequencies were compared between CHD and non-CHD individuals using the CLUMP22 software with 10,000 Monte Carlo simulations.
  
  
  
  Allelic tests showed that PLA2G7 rs9395208 and CD40 rs1800686 were significantly associated with CHD. Moreover, IL1B rs16944, PLA2G7 rs9395208, and CD40 rs1800686 were shown to be associated with CHD under the dominant model. Further gender-based subgroup tests showed that one SNP (CD40 rs1800686) and two SNPs (FAM5C rs12732361 and CD36 rs2065666) were associated with CHD in females and males, respectively. And the age-based subgroup tests indicated that PLA2G7 rs9395208, IL1B rs16944, and CD40 rs1800686 were associated with CHD among individuals younger than 55, younger than 65, and over 65, respectively.
  
  
  
  In conclusion, all the six inflammation-related CpG-SNPs (rs16944, rs2071008, rs12732361, rs2065666, rs9395208, and rs1800686) were associated with CHD in the combined or subgroup tests, suggesting an important role of inflammation in the risk of CHD.
authors.affiliation: Cardiovascular Center of Ningbo First Hospital, Ningbo University, Ningbo, Zhejiang, 315010, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Medicine, Ningbo University, Ningbo, Zhejiang, 315211, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Medicine, Ningbo University, Ningbo, Zhejiang, 315211, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Texas Advanced Computing Center, University of Texas at Austin, 10100 Burnet Road (R8700), Austin, TX, 78758-4497, USA.
authors.forename: William
authors.initials: W
authors.lastname: Yang
authors.affiliation: Cardiovascular Center of Ningbo First Hospital, Ningbo University, Ningbo, Zhejiang, 315010, China.|School of Medicine, Ningbo University, Ningbo, Zhejiang, 315211, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.affiliation: School of Medicine, Ningbo University, Ningbo, Zhejiang, 315211, China.
authors.forename: Huadan
authors.initials: H
authors.lastname: Ye
authors.affiliation: MidSouth Bioinformatics Center, Department of Information Science, George Washington Donaghey College of Engineering and Information Science, and Joint Bioinformatics Graduate Program, University of Arkansas at Little Rock and University of Arkansas for Medical Sciences, 2881 S. University Ave, Little Rock, AR, 72204, USA.
authors.forename: Jack Y
authors.initials: JY
authors.lastname: Yang
authors.affiliation: School of Medicine, Ningbo University, Ningbo, Zhejiang, 315211, China.
authors.forename: Qingxiao
authors.initials: Q
authors.lastname: Hong
authors.affiliation: Center of Safety Evaluation, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, 310007, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Xin
authors.affiliation: MidSouth Bioinformatics Center, Department of Information Science, George Washington Donaghey College of Engineering and Information Science, and Joint Bioinformatics Graduate Program, University of Arkansas at Little Rock and University of Arkansas for Medical Sciences, 2881 S. University Ave, Little Rock, AR, 72204, USA.
authors.forename: Mary Qu
authors.initials: MQ
authors.lastname: Yang
authors.affiliation: Medical College, Wuhan University of Science and Technology, Wuhan, 430064, China.|Department of Internal Medicine and Biochemistry, Rush University Medical Center, Chicago, IL, 60612, USA.
authors.forename: Youping
authors.initials: Y
authors.lastname: Deng
authors.affiliation: School of Medicine, Ningbo University, Ningbo, Zhejiang, 315211, China. duanshiwei@nbu.edu.cn.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Duan
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000235:genetics; D000368:Aged; D044466:Asian People / Q000235:genetics; D018955:CD36 Antigens / Q000235:genetics; D019013:CD40 Antigens / Q000235:genetics; D002681:China; D003327:Coronary Disease / Q000208:ethnology / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007249:Inflammation / Q000208:ethnology / Q000235:genetics*; D053583:Interleukin-1beta / Q000235:genetics; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D053574:Receptors, Interleukin-1 Type II / Q000235:genetics; D012307:Risk Factors
chemical_list: C528897:BRINP3 protein, human; D018955:CD36 Antigens; D019013:CD40 Antigens; D004268:DNA-Binding Proteins; C508592:IL1R2 protein, human; D053583:Interleukin-1beta; D053574:Receptors, Interleukin-1 Type II; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; C526684:PLA2G7 protein, human
keywords: Coronary heart disease; CpG-SNP; Inflammation; Polymorphism; Promoter