title: Exenatide Alters Gene Expression of Neural Cell Adhesion Molecule (NCAM), Intercellular Cell Adhesion Molecule (ICAM), and Vascular Cell Adhesion Molecule (VCAM) in the Hippocampus of Type 2 Diabetic Model Mice.
abstract: BACKGROUND Glucagon-like peptide-1 (GLP-1), a potent and selective agonist for the GLP-1 receptor, ameliorates the symptoms of diabetes through stimulation of insulin secretion. Exenatide is a potent and selective agonist for the GLP-1 receptor. Cell adhesion molecules are members of the immunoglobulin superfamily and are involved in synaptic rearrangements in the mature brain. MATERIAL AND METHODS The present study demonstrated the effects of exenatide treatment (0.1 µg/kg, subcutaneously, twice daily for 2 weeks) on the gene expression levels of cell adhesion molecules, neural cell adhesion molecule (NCAM), intercellular cell adhesion molecule (ICAM), and vascular cell adhesion molecule (VCAM) in the brain tissue of diabetic BALB/c male mice by real-time quantitative polymerase chain reaction (PCR). Diabetes was induced by streptozotocin/nicotinamide (STZ-NA) injection to male mice. RESULTS The results of this study revealed that hippocampal gene expression of NCAM, ICAM, and VCAM were found to be up-regulated in STZ-NA-induced diabetic mice compared to those of controls. A significant decrease in the gene expression levels of NCAM, ICAM, and VCAM were determined after 2 weeks of exenatide administration. CONCLUSIONS Cell adhesion molecules may be involved in the molecular mechanism of diabetes. Exenatide has a strong beneficial action in managing diabetes induced by STZ/NA by altering gene expression of NCAM, ICAM, and VCAM.
authors.affiliation: Department of Genetics, Kocaeli University, Medical Faculty, Kocaeli, Turkey.
authors.forename: Esen
authors.initials: E
authors.lastname: Gumuslu
authors.affiliation: Department of Genetics, Kocaeli University, Medical Faculty, Kocaeli, Turkey.
authors.forename: Naci
authors.initials: N
authors.lastname: Cine
authors.affiliation: Department of Genetics, Kocaeli University, Medical Faculty, Kocaeli, Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Ertan Gökbayrak
authors.affiliation: Department of Pharmacology, Kocaeli University, Medical Faculty, Kocaeli, Turkey.
authors.forename: Oguz
authors.initials: O
authors.lastname: Mutlu
authors.affiliation: Department of Pharmacology, Kocaeli University, Medical Faculty, Kocaeli, Turkey.
authors.forename: Ipek
authors.initials: I
authors.lastname: Komsuoglu Celikyurt
authors.affiliation: Department of Pharmacology, Kocaeli University, Medical Faculty, Kocaeli, Turkey.
authors.forename: Guner
authors.initials: G
authors.lastname: Ulak
keywords: Diabetes Mellitus; Genetic Association Studies; Mice, Inbred BALB C