title: Evolutionary and functional analysis of mulberry type III polyketide synthases.
abstract: Type III polyketide synthases are important for the biosynthesis of flavonoids and various plant polyphenols. Mulberry plants have abundant polyphenols, but very little is known about the mulberry type III polyketide synthase genes. An analysis of these genes may provide new targets for genetic improvement to increase relevant secondary metabolites and enhance the plant tolerance to biotic and abiotic stresses.
  
  
  
  Eighteen genes encoding type III polyketide synthases were identified, including six chalcone synthases (CHS), ten stilbene synthases (STS), and two polyketide synthases (PKS). Functional characterization of four genes representing most of the MnCHS and MnSTS genes by coexpression with 4-Coumaroyl-CoA ligase in Escherichia coli indicated that their products were able to catalyze p-coumaroyl-CoA and malonyl-CoA to generate naringenin and resveratrol, respectively. Microsynteny analysis within mulberry indicated that segmental and tandem duplication events contributed to the expansion of the MnCHS family, while tandem duplications were mainly responsible for the generation of the MnSTS genes. Combining the evolution and expression analysis results of the mulberry type III PKS genes indicated that MnCHS and MnSTS genes evolved mainly under purifying selection to maintain their original functions, but transcriptional subfunctionalization occurred during long-term species evolution. Moreover, mulberry leaves can rapidly accumulated oxyresveratrol after UV-C irradiation, suggesting that resveratrol was converted to oxyresveratrol.
  
  
  
  Characterizing the functions and evolution of mulberry type III PKS genes is crucial for advancing our understanding of these genes and providing the basis for further studies on the biosynthesis of relevant secondary metabolites in mulberry plants.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing, 400715, People's Republic of China.
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing, 400715, People's Republic of China.
authors.forename: Jiubo
authors.initials: J
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing, 400715, People's Republic of China.
authors.forename: Hu
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing, 400715, People's Republic of China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing, 400715, People's Republic of China.
authors.forename: Bi
authors.initials: B
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing, 400715, People's Republic of China. hejia@swu.edu.cn.
authors.forename: Ningjia
authors.identifier: 0000-0002-7322-7113
authors.initials: N
authors.lastname: He
mesh_terms: D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005075:Biological Evolution; D053898:Biosynthetic Pathways; D016000:Cluster Analysis; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D008958:Models, Molecular; D031625:Morus / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects; D005810:Multigene Family; D016366:Open Reading Frames; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D011487:Protein Conformation; D013267:Stilbenes / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D013267:Stilbenes; D000217:Acyltransferases; C020141:flavanone synthetase
keywords: Chalcone synthase; Evolutionary analysis; Functional analysis; Gene expression; Mulberry; Stilbene synthase