title: Antioxidant and antitumor efficacy of Luteolin, a dietary flavone on benzo(a)pyrene-induced experimental lung carcinogenesis.
abstract: The present study is designed to assess the antioxidant and antitumor potential of luteolin against benzo(a)pyrene [B(a)P]-induced lung carcinogenesis in Swiss albino mice. Here, we reported that oral administration of B(a)P (50mg/kg body weight) to mice resulted in raised lipid peroxides (LPO), lung specific tumor markers such as carcinoembryonic antigen (CEA) and neuron specific enolase (NSE) with concomitant decrease in the levels of both enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione-s-transferase (GST), and non-enzymatic antioxidants such as reduced glutathione (GSH), vitamin E and vitamin C. Luteolin treatment (15mg/kg body weight, p.o) significantly counteracted all these alterations and maintained cellular normalcy. Moreover, assessment of protein expression levels by western blot analysis revealed that luteolin treatment effectively negates B(a)P-induced upregulated expression of proliferating cell nuclear antigen (PCNA), cytochrome P450 1A1 (CYP1A1) and nuclear factor-kappa B (NF-κB). Furthermore, histopathology of lung tissue and immunohistochemistry of CYP1A1 were carried out to substantiate the anti- lung cancer effect of luteolin. Overall, these findings confirm the chemopreventive potential of luteolin against B(a)P induced lung carcinogenesis.
authors.affiliation: Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Guwahati, Bhangagarh, 781032, Assam, India. Electronic address: ishreddy4@gmail.com.
authors.forename: Eshvendar Reddy
authors.initials: ER
authors.lastname: Kasala
authors.affiliation: Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Guwahati, Bhangagarh, 781032, Assam, India.
authors.forename: Lakshmi Narendra
authors.initials: LN
authors.lastname: Bodduluru
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Sciences, Assam Agricultural University, Guwahati, 781022, Assam, India.
authors.forename: Chandana C
authors.initials: CC
authors.lastname: Barua
authors.affiliation: Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Guwahati, Bhangagarh, 781032, Assam, India.
authors.forename: Ranadeep
authors.initials: R
authors.lastname: Gogoi
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001564:Benzo(a)pyrene; D014408:Biomarkers, Tumor / Q000097:blood; D001835:Body Weight / Q000187:drug effects; D002272:Carcinoembryonic Antigen / Q000097:blood; D063646:Carcinogenesis / Q000187:drug effects / Q000473:pathology*; D004032:Diet; D015151:Immunoblotting; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D047311:Luteolin / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010751:Phosphopyruvate Hydratase / Q000097:blood
chemical_list: D016588:Anticarcinogenic Agents; D000975:Antioxidants; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D009363:Neoplasm Proteins; D001564:Benzo(a)pyrene; D010751:Phosphopyruvate Hydratase; D047311:Luteolin
keywords: Benzo(a)pyrene; Chemoprevention; Dietary flavone; Lung cancer; Luteolin