title: Single-wall and multi-wall carbon nanotubes promote rice root growth by eliciting the similar molecular pathways and epigenetic regulation.
abstract: Organisms are constantly exposed to environmental stimuli and have evolved mechanisms of protection and adaptation. Various effects of nanoparticles (NPs) on crops have been described and some results confirm that NPs could enhance plant growth at the physiological and genetic levels. This study comparatively analysed the effect of carbon nanotubes (CNTs) on rice growth. The results showed that single-wall CNTs were located in the intercellular space while multi-wall CNTs penetrated cell walls in roots. CNTs could promote rice root growth through the regulation of expression of the root growth related genes and elevated global histone acetylation in rice root meristem zones. These responses were returned to normal levels after CNTs were removed from medium. CNTs caused the similar histone acetylation and methylation statuses across the local promoter region of the Cullin-RING ligases 1 (CRL1) gene and increased micrococcal nuclease accessibility of this region, which enhanced this gene expression. The authors results suggested that CNTs could cause plant responses at the cellular, genetic, and epigenetic levels and these responses were independent on interaction modes between root cells and CNTs.
authors.affiliation: Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, People's Republic of China.
authors.forename: Shihan
authors.initials: S
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Synergy Innovation Center of Biological Peptide Antidiabetics of Hubei Province, School of Life Science, Wuchang University of Technology, Wuhan 430223, People's Republic of China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Yapei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Haoli
authors.initials: H
authors.lastname: Hou
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, No. 2 Tiansheng Road, BeiBei District, Chongqing 400715, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China. ljli@whu.edu.cn.
authors.forename: Lijia
authors.initials: L
authors.lastname: Li
mesh_terms: D002244:Carbon / Q000008:administration & dosage* / Q000737:chemistry; D044127:Epigenesis, Genetic; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000502:physiology; D008422:Materials Testing; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D014018:Tissue Distribution
chemical_list: D037742:Nanotubes, Carbon; D010940:Plant Proteins; D002244:Carbon