title: Combinatorial epigenetic mechanisms and efficacy of early breast cancer inhibition by nutritive botanicals.
abstract: Aberrant epigenetic events are important contributors to the pathogenesis of different types of cancers and dietary botanicals with epigenetic properties can influence early cancer development leading to cancer prevention effects. We sought to investigate potential combinatorial effects of bioactive dietary components including green tea polyphenols (GTPs) and broccoli sprouts (BSp) on neutralizing epigenetic aberrations during breast tumorigenesis.
  
  
  
  The combinatorial effects were evaluated in a breast cancer transformation cellular system and breast cancer mouse xenografts.
  
  
  
  Combined treatment with epigallocatechin-3-gallate in GTPs and sulforaphane in BSp resulted in a synergistic inhibition of breast cancer cellular growth. Further studies revealed this combination led to genome-wide epigenetic alterations. Combinatorial diets significantly inhibited tumor growth in breast cancer mouse xenografts. Collectively, these studies indicate that combined GTPs and BSp are highly effective in inhibiting early breast cancer development by, at least in part, regulating epigenetic mechanisms.
authors.affiliation: Department of Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.|Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA.|Nutrition Obesity Research Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Drug Discovery & Biomedical Sciences, University of South Carolina, Columbia, SC 29208, USA.
authors.forename: Phillip
authors.initials: P
authors.lastname: Buckhaults
authors.affiliation: Department of Biostatistics, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Xiangqin
authors.initials: X
authors.lastname: Cui
authors.affiliation: Department of Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.|Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA.|Nutrition Obesity Research Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA.|Comprehensive Center for Healthy Aging, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Trygve O
authors.initials: TO
authors.lastname: Tollefsbol
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D001937:Brassica / Q000737:chemistry; D001943:Breast Neoplasms / Q000188:drug therapy / Q000235:genetics*; D063646:Carcinogenesis / Q000187:drug effects / Q000235:genetics; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004359:Drug Therapy, Combination; D044127:Epigenesis, Genetic; D005260:Female; D006801:Humans; D017879:Isothiocyanates / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D008819:Mice, Nude; D013454:Sulfoxides; D013662:Tea / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017879:Isothiocyanates; D013454:Sulfoxides; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate; C016766:sulforaphane
keywords: EGCG; breast tumorigenesis; broccoli sprouts; epigenetic; green tea polyphenols; sulforaphane