title: Ack promotes tissue growth via phosphorylation and suppression of the Hippo pathway component Expanded.
abstract: Non-receptor tyrosine kinase activated cdc42 kinase was reported to participate in several types of cancers in mammals. It is also believed to have an anti-apoptotic function in Drosophila. Here, we report the identification of Drosophila activated cdc42 kinase as a growth promoter and a novel Hippo signaling pathway regulator. We find that activated cdc42 kinase promotes tissue growth through modulating Yorkie activity. Furthermore, we demonstrate that activated cdc42 kinase interacts with Expanded and induces tyrosine phosphorylation of Expanded on multiple sites. We propose a model that activated cdc42 kinase negatively regulates Expanded by changing its phosphorylation status to promote tissue growth. Moreover, we show that ack genetically interacts with merlin and expanded. Thus, we identify Drosophila activated cdc42 kinase as a Hippo pathway regulator.
authors.affiliation: State Key Laboratory of Cell Biology, Innovation Center for Cell Signaling Network, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences , Shanghai, China.
authors.forename: Lianxin
authors.initials: L
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Cell Biology, Innovation Center for Cell Signaling Network, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences , Shanghai, China.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Cell Biology, Innovation Center for Cell Signaling Network, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences , Shanghai, China.
authors.forename: Meng-Xin
authors.initials: MX
authors.lastname: Yin
authors.affiliation: Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign , Urbana, IL, USA.
authors.forename: Liguo
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Cell Biology, Innovation Center for Cell Signaling Network, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences , Shanghai, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Cell Biology, Innovation Center for Cell Signaling Network, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences , Shanghai, China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Wu
authors.affiliation: School of Life Sciences, Tsinghua University , Beijing, China.
authors.forename: Zhaoyu
authors.initials: Z
authors.lastname: Xue
authors.affiliation: Department of Anatomy and Neurobiology, School of Medicine, Tongji University , Shanghai, China.
authors.forename: Margaret S
authors.initials: MS
authors.lastname: Ho
authors.affiliation: School of Life Sciences, Tsinghua University , Beijing, China.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Cell Biology, Innovation Center for Cell Signaling Network, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Cell Biology, Innovation Center for Cell Signaling Network, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
keywords: Hippo pathway; Yorkie; activated cdc43 kinase; expanded