title: Indirubin-3'-monoxime suppresses amyloid-beta-induced apoptosis by inhibiting tau hyperphosphorylation.
abstract: Indirubin-3'-monoxime is an effective inhibitor of cyclin-dependent protein kinases, and may play an obligate role in neuronal apoptosis in Alzheimer's disease. Here, we found that indirubin-3'-monoxime improved the morphology and increased the survival rate of SH-SY5Y cells exposed to amyloid-beta 25-35 (Aβ25-35), and also suppressed apoptosis by reducing tau phosphorylation at Ser199 and Thr205. Furthermore, indirubin-3'-monoxime inhibited phosphorylation of glycogen synthase kinase-3β (GSK-3β). Our results suggest that indirubin-3'-monoxime reduced Aβ25-35-induced apoptosis by suppressing tau hyperphosphorylation via a GSK-3β-mediated mechanism. Indirubin-3'-monoxime is a promising drug candidate for Alzheimer's disease.
authors.affiliation: Department of Neurology, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Shu-Gang
authors.initials: SG
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Xiao-Shan
authors.initials: XS
authors.lastname: Wang
authors.affiliation: Department of Neurology, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China; Department of Neurology, Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Ying-Dong
authors.identifier: 0000-0002-6470-5064
authors.initials: YD
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Di
authors.affiliation: Department of Neurology, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Jing-Ping
authors.initials: JP
authors.lastname: Shi
authors.affiliation: Department of Neurology, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Qian
authors.affiliation: Department of Neurology, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Li-Gang
authors.initials: LG
authors.lastname: Xu
authors.affiliation: Department of Neurology, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Xing-Jian
authors.initials: XJ
authors.lastname: Lin
authors.affiliation: Department of Neurology, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
keywords: Alzheimer's disease; amyloid-beta; indirubin-3′-monoxime; nerve regeneration; neural regeneration; neuronal apoptosis; phosphorylated c-Jun N-terminal kinase; phosphorylated glycogen synthase kinase-3β; tau hyperphosphorylation