title: Effects of Regular Treadmill Exercise on a DNA Oxidative-Damage Marker and Total Antioxidant Capacity in Rat Hippocampal Tissue.
abstract: OBJECTIVE
  Regular exercise can result in changes in the levels of oxidative stress in the hippocampus; however, little attention has been paid to physical-activity-induced neuronal protection to exposure to lead compounds. This study investigated the effects of regular treadmill exercise on a DNA oxidative-damage marker [8-hydroxy-2'-deoxyguanosine (8-OHdG)] and the total antioxidant capacity (TAC) of hippocampal tissue in lead-acetate exposed rats.
  
  
  METHODS
  This study investigated the effects of 8 weeks of regular treadmill exercise on 8-OHdG and the TAC of hippocampal tissue in lead-acetate-exposed rats. Wistar rats were randomly divided into four groups: baseline, sham (control), lead, and exercise+lead. The exercise program involved running on a treadmill with increasing intensity five times a week for 8 weeks. Animals in the lead and exercise+lead groups received lead acetate at 20 mg/kg body weight intraperitoneally three times weekly for 8 weeks. Animals in the sham group received solvent (ethyl oleate) at 30 mg/kg body weight three times weekly for 8 weeks. TAC and 8-OHdG were measured by spectrophotometric and ELISA techniques, respectively. Data were analyzed by ANOVA and Tukey's post-hoc test with a significance cutoff of p≤0.05.
  
  
  RESULTS
  The level of 8-OHdG and the TAC were significantly higher and lower, respectively, in the lead group than in the baseline and sham groups (p<0.01). However, the 8-OHdG level and TAC value in hippocampal tissue were significantly decreased and increased, respectively, in the exercise+lead group relative to the lead group (p<0.05).
  
  
  CONCLUSIONS
  The TAC of hippocampal tissue may be directly associated with neural protection mechanisms of exercise following lead acetate injection, and the beneficial effects of regular exercise in preventing hippocampal neuronal damage could be due to decreased hippocampal oxidative stress such as reflected by a lower 8-OHdG level and increased TAC.
authors.affiliation: Cellular and Molecular Biology Research Center, Health Research Center, Babol University of Medical Sciences, Babol, Iran.|Department of Clinical Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Soleiman
authors.initials: S
authors.lastname: Mahjoub
authors.affiliation: Department of Chemical Engineering, Islamic Azad University, Ayatollah Amoli Branch, Amol, Iran.
authors.forename: Arezoo
authors.initials: A
authors.lastname: Ghadi
authors.affiliation: Cellular and Molecular Biology Research Center, Health Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Roghayeh
authors.initials: R
authors.lastname: Pourbagher
authors.affiliation: Department of Biostatistics and Epidemiology, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Karimollah
authors.initials: K
authors.lastname: Hajian-Tilaki
authors.affiliation: Infectious Diseases and Tropical Medicine Research Center, Health Research Center, Babol University of Medical Sciences, Babol, Iran. masrour_j@yahoo.com.
authors.forename: Jila
authors.initials: J
authors.lastname: Masrour-Roudsari
keywords: 8-OHdG; hippocampus; lead acetate; oxidative stress; regular treadmill exercise; total antioxidant capacity