title: Expression of a mitochondrial gene orfH79 from CMS-Honglian rice inhibits Escherichia coli growth via deficient oxygen consumption.
abstract: Cytoplasmic male sterility (CMS) has often been associated with abnormal mitochondrial open frames (ORF), orfH79 is a mitochondrial chimeric gene responsible for the CMS trait in Honglian (HL) rice. In this study, the weakly produced ORFH79 protein significantly inhibited the growth of E. coli in an oxygen culture, however, the growth of the transformants producing ORFH79 was indistinguishable from the control under anaerobic incubation conditions. In addition, a lower respiration rate, wrinkled bacterial surfaces, and decreased pyruvate kinase and α-ketoglutarate dehydrogenase activities were observed in the ORFH79 produced E. coli. These results indicate that ORFH79 impairs the oxygen respiration of E. coli, which may inhibit E. coli growth.
authors.affiliation: Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang, 330031 People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Ding
authors.affiliation: Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang, 330031 People's Republic of China.
authors.forename: Qiusheng
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang, 330031 People's Republic of China.
authors.forename: Canming
authors.initials: C
authors.lastname: Bao
authors.affiliation: Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang, 330031 People's Republic of China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Ai
authors.affiliation: Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang, 330031 People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang, 330031 People's Republic of China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Li
authors.affiliation: Jiangxi Super-Rice Research and Development Center, Nanchang, 330200 People's Republic of China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Xie
authors.affiliation: Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang, 330031 People's Republic of China.
authors.forename: Youlin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Jiangxi Super-Rice Research and Development Center, Nanchang, 330200 People's Republic of China.
authors.forename: Yaohui
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang, 330031 People's Republic of China.
authors.forename: Xiaojue
authors.initials: X
authors.lastname: Peng
keywords: Cytoplasmic male sterility; E. coli growth; Oxygen respiration; orfH79