title: Ochratoxin A Detection on Antibody- Immobilized on BSA-Functionalized Gold Electrodes.
abstract: Ochratoxin A (OTA)-a toxin produced by Aspergillus carbonarius, Aspergillus ochraceus, and Penicillium verrucosum-is one of the most-abundant food-contaminating mycotoxins. To avoid the risk of OTA consumption for humans and animals, the rapid detection and quantitation of OTA level in different commodities are of great importance. In this work, an impedimetric immunosensor for ochratoxin A (OTA) detection, a common toxic botanical contaminant, was developed via the immobilization of anti-OTA antibody on bovine serum albumin modified gold electrodes. A four-step reaction protocol was tested to modify the gold electrode and obtain the sensing substrate. All the steps of the immunosensor elaboration and also the immunochemical reaction between surface-bound antibody and ochratoxin A were analyzed using cyclic voltammetry and electrochemical impedance spectroscopy. Modification of the impedance due to the specific antigen-antibody reaction at immunosensor surface, was used in order to detect ochratoxin A. Linear proportionality of the charge transfer resistance to the concentration of OTA allows ochratoxin A detection in the range of 2.5-100 ng/mL.
authors.affiliation: Fundamental, Prophylactic and Clinical Specialties Department, Transilvania University of Brasov, Brasov, Romania.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Badea
authors.affiliation: Automation and Information Technology Department, Transilvania University of Brasov, Romania.
authors.forename: Laura
authors.initials: L
authors.lastname: Floroian
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milano, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Restani
authors.affiliation: Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania.
authors.forename: Simona Codruta Aurora
authors.initials: SC
authors.lastname: Cobzac
authors.affiliation: Department of Medical And Surgical Specialties, Transilvania University of Brasov, Brasov, Romania.
authors.forename: Marius
authors.initials: M
authors.lastname: Moga
mesh_terms: D000906:Antibodies / Q000276:immunology*; D015374:Biosensing Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D000906:Antibodies; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A; D012710:Serum Albumin, Bovine; D006046:Gold