title: Examination and Disruption of the Yeast Cell Wall.
abstract: The cell wall of Saccharomyces cerevisiae is a complicated extracellular organelle. Although the barrier may seem like a technical nuisance for researchers studying intracellular biomolecules or conditions, the rigid wall is an essential aspect of the yeast cell. Without it, yeast cells are unable to proliferate or carry out their life cycle. The chemical composition of the cell wall and the biosynthetic pathways and signal transduction mechanisms involved in cell wall remodeling have been studied extensively, but many unanswered questions remain. This introduction describes techniques for investigating abnormalities in the cell and spore walls and performing cell wall disruption.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture 277-8562, Japan;
authors.forename: Hiroki
authors.initials: H
authors.lastname: Okada
authors.affiliation: Department of Cell Biology, Graduate School of Medical Sciences, Nagoya City University, 1, Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi Prefecture 467-8601, Japan;
authors.forename: Keiko
authors.initials: K
authors.lastname: Kono
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794-5215.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Neiman
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture 277-8562, Japan;
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ohya
mesh_terms: D002473:Cell Wall / Q000737:chemistry* / Q000502:physiology*; D008828:Microbiological Techniques / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000502:physiology*