title: Purification, characterization and in vitro antioxidant activities of polysaccharide from Chaenomeles speciosa.
abstract: A water-soluble polysaccharide named as CSP-2 was isolated and purified from Chaenomeles speciosa by DEAE-Sepharose and Sephadex G-100 column chromatography. CSP-2 was composed of galactose (Gal), rhamnose (Rha), glucose (Glc) and xylose (Xyl) with a relative molar ratio of 3.8:1.6:1.2:0.4. The average molecular weight of CSP-2 was estimated to be about 4.6×104Da. The in vitro antioxidant assay showed that CSP-2 exhibited remarkable antioxidant activities to scavenge the DPPH radical, superoxide radical, hydroxyl radical and ABTS radical and significant cytoprotective effects on H2O2-induced PC12 cells injury in a concentration-dependent manner. Overall, CSP-2 may be useful as a naturally potential antioxidant agent for application in food and medicinal fields.
authors.affiliation: Department of Pharmacology, Basic Medical School, Wuhan University, Wuhan 430072, PR China; College of Life Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan 430072, PR China. Electronic address: xiexianfei001@163.com.
authors.forename: Xianfei
authors.initials: X
authors.lastname: Xie
authors.affiliation: College of Life Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan 430072, PR China.
authors.forename: Guolin
authors.initials: G
authors.lastname: Zou
authors.affiliation: College of Life Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan 430072, PR China.
authors.forename: Chenghai
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D052160:Benzothiazoles / Q000037:antagonists & inhibitors; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D008970:Molecular Weight; D016716:PC12 Cells; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D012210:Rhamnose / Q000737:chemistry; D027824:Rosaceae / Q000737:chemistry*; D012995:Solubility; D013451:Sulfonic Acids / Q000037:antagonists & inhibitors; D013481:Superoxides / Q000037:antagonists & inhibitors; D014867:Water; D014994:Xylose / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D011134:Polysaccharides; D013451:Sulfonic Acids; D014867:Water; D013481:Superoxides; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; D014994:Xylose; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D005947:Glucose; D012210:Rhamnose; D005690:Galactose
keywords: Antioxidant activities; Chaenomeles speciosa; Polysaccharides