title: Sulfated polysaccharide from the marine algae Hypnea musciformis inhibits TNBS-induced intestinal damage in rats.
abstract: Sulfated polysaccharides extracted from seaweed have important pharmacological properties. Thus, the aim of this study was to characterize the sulfated polysaccharide (PLS) from the algae Hypnea musciformis and evaluate its protective effect in colitis induced by trinitrobenzene sulfonic acid in rats. The sulfated polysaccharide possess a high molecular mass (1.24×10(5)gmol(-1)) and is composed of a κ-carrageenan, as depicted by FT-IR and NMR spectroscopic data. PLS was administered orally (10, 30, and 60mg/kg, p.o.) for three days, starting before TNBS (trinitrobenzene sulfonic acid) instillation (day 1). The rats were killed on day three, the portion of distal colon (5cm) was excised and evaluated macroscopic scores and wet weight. Then, samples of the intestinal were used for histological evaluation and quantification of glutathione, malonyldialdehyde acid, myeloperoxidase, nitrate/nitrite and cytokines. Our results demonstrate that PLS reduced the colitis and all analyzed biochemical parameters. Thus, we concluded that the PLS extracted from the marine algae H. musciformis reduced the colitis in animal model and may have an important promising application in the inflammatory bowel diseases.
authors.affiliation: LAFFEX-Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Parnaíba, 64202-020 PI, Brazil.
authors.forename: Tarcisio
authors.initials: T
authors.lastname: V Brito
authors.affiliation: Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, 60455-760 CE, Brazil; Federal Institute of Education, Science and Technology of Ceará, Juazeiro do Norte, CE, Brazil.
authors.forename: Francisco C N
authors.initials: FCN
authors.lastname: Barros
authors.affiliation: LEFFAG-Laboratory of Physiopharmacology Study of Gastrointestinal Tract, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Renan O
authors.initials: RO
authors.lastname: Silva
authors.affiliation: LAFFEX-Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Parnaíba, 64202-020 PI, Brazil.
authors.forename: Genilson J
authors.initials: GJ
authors.lastname: Dias Júnior
authors.affiliation: LAFFEX-Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Parnaíba, 64202-020 PI, Brazil.
authors.forename: José Simião
authors.initials: JS
authors.lastname: C Júnior
authors.affiliation: LEFFAG-Laboratory of Physiopharmacology Study of Gastrointestinal Tract, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Álvaro X
authors.initials: ÁX
authors.lastname: Franco
authors.affiliation: LEFFAG-Laboratory of Physiopharmacology Study of Gastrointestinal Tract, Federal University of Ceará, Fortaleza, CE, Brazil; Department of Morphology, Medical School, Federal University of Ceara, Rua Delmiro de Farias s/n, Rodolfo Teofilo, Fortaleza, CE CEP 60416-030, Brazil.
authors.forename: Pedro M G
authors.initials: PMG
authors.lastname: Soares
authors.affiliation: Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, 60455-760 CE, Brazil.
authors.forename: Luciano S
authors.initials: LS
authors.lastname: Chaves
authors.affiliation: Laboratory of Polymers, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, 60455-760 CE, Brazil.
authors.forename: Clara M W S
authors.initials: CMWS
authors.lastname: Abreu
authors.affiliation: Laboratory of Polymers, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, 60455-760 CE, Brazil.
authors.forename: Regina C M
authors.initials: RCM
authors.lastname: de Paula
authors.affiliation: LEFFAG-Laboratory of Physiopharmacology Study of Gastrointestinal Tract, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Marcellus H L P
authors.initials: MHLP
authors.lastname: Souza
authors.affiliation: Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, 60455-760 CE, Brazil.
authors.forename: Ana Lúcia P
authors.initials: ALP
authors.lastname: Freitas
authors.affiliation: LAFFEX-Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Parnaíba, 64202-020 PI, Brazil. Electronic address: andreluiz@ufpi.edu.br.
authors.forename: André Luiz
authors.initials: AL
authors.lastname: R Barbosa
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D019610:Cytoprotection / Q000187:drug effects; D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008297:Male; D020556:Neutrophil Infiltration / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013431:Sulfates / Q000737:chemistry*; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects*
chemical_list: D016207:Cytokines; D005685:Galactans; D013431:Sulfates; D014302:Trinitrobenzenesulfonic Acid
keywords: Colitis; Inflammatory disease; Marine algae; Sulfated polysaccharide