title: A simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion RNA.
abstract: The TMPRSS2:ERG gene fusion is one of a series of highly promising prostate cancer (PCa) biomarker alternatives to the controversial serum PSA. Current methods for detecting TMPRSS2:ERG are limited in terms of long processing time, high cost and the need for specialized equipment. Thus, there is an unmet need for less complex, faster, and cheaper methods to enable gene fusion detection in the clinic. We describe herein a simple, rapid and inexpensive assay which combines robust isothermal amplification technique with a novel visualization method for evaluating urinary TMPRSS2:ERG status at less than USD 5 and with minimal equipment. The assay is sensitive, and rapidly detects as low as 10(5) copies of TMPRSS2:ERG transcripts while maintaining high levels of specificity.
authors.affiliation: Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, QLD 4072, Australia.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Koo
authors.affiliation: Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, QLD 4072, Australia.
authors.forename: Eugene J H
authors.initials: EJ
authors.lastname: Wee
authors.affiliation: Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, QLD 4072, Australia.
authors.forename: Paul N
authors.initials: PN
authors.lastname: Mainwaring
authors.affiliation: Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, QLD 4072, Australia.|School of Chemistry and Molecular Biosciences, The University of Queensland, QLD 4072, Australia.
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000652:urine; D003365:Costs and Cost Analysis; D050939:Gene Fusion; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods*; D011471:Prostatic Neoplasms / Q000175:diagnosis*; D012333:RNA, Messenger / Q000235:genetics / Q000652:urine*; D012680:Sensitivity and Specificity; D012697:Serine Endopeptidases / Q000235:genetics*; D013997:Time Factors; D000071230:Transcriptional Regulator ERG / Q000235:genetics; D016482:Urinalysis / Q000191:economics / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; C072312:ERG protein, human; D012333:RNA, Messenger; D000071230:Transcriptional Regulator ERG; D012697:Serine Endopeptidases; C421305:TMPRSS2 protein, human