title: LC-MS profiling of glucosinolates in the seeds of Brassica elongata Ehrh., and of the two stenoendemic B. botteri Vis and B. cazzae Ginzb. & Teyber.
abstract: The glucosinolates (GLs) present in seed extracts of Brassica elongata Ehrh., B. botteri Vis and B. cazzae Ginzb. & Teyber from Croatia were identified by LC-MS. 4-Hydroxyindol-3-ylmethyl GL (3) was the major GL in the seeds of B. elongata, along with the four minor GLs 2-(R)-hydroxy-3-butenyl- (1), 3-butenyl- (2), 4-pentenyl- (4) and indol-3-ylmethyl (5). The seeds of B. botteri (Vis island) and B. cazzae (Sušac island) contained 2 as the major GL as well as 1, 3, 5 and 4-methoxyindol-3-ylmethyl GL (6). However, the GLs in B. botteri (Palagruža island) differed from other varieties having 2-propenyl GL (7) as the major GL in the seeds, and the four minor GLs 2, 3, 5 and 6. This first report of the GL content in the seeds of B. elongata, B. botteri and B. cazzae indicates that the unique GL profiles could be specific to the geographical origin of the plant.
authors.affiliation: a Department of Chemistry and Biochemistry, Biomolecular Sciences Programme , Laurentian University , Sudbury , Canada.
authors.forename: Sabine
authors.initials: S
authors.lastname: Montaut
authors.affiliation: b Faculty of Chemistry and Technology, Department of Organic Chemistry , University of Split , Split , Croatia.
authors.forename: Ivica
authors.initials: I
authors.lastname: Blažević
authors.affiliation: c Faculty of Science, Department of Biology , University of Split , Split , Croatia.
authors.forename: Mirko
authors.initials: M
authors.lastname: Ruščić
authors.affiliation: d Université d'Orléans et CNRS, ICOA, UMR 7311 , Orléans , France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Rollin
mesh_terms: D001937:Brassica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D017523:Croatia; D005961:Glucosinolates / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: C000618177:4-hydroxyindol-3-ylmethyl GL; D005961:Glucosinolates; D007211:Indoles; D010936:Plant Extracts
keywords: Brassica botteri; Brassica cazzae; Brassica elongata; Brassica incana; LC–MS; glucosinolates