title: Lichen secondary metabolite evernic acid as potential quorum sensing inhibitor against Pseudomonas aeruginosa.
abstract: Cystic Fibrosis is a genetic disease and it affects the respiratory and digestive systems. Pseudomonas aeruginosa infections in Cystic Fibrosis are presented as the main cause for high mortality and morbidity rates. Pseudomonas aeruginosa populations can regulate their virulence gene expressions via the bacterial communication system: quorum sensing. Inhibition of quorum sensing by employing quorum sensing inhibitors can leave the bacteria vulnerable. Therefore, determining natural sources to obtain potential quorum sensing inhibitors is essential. Lichens have ethnobotanical value for their medicinal properties and it is possible that their secondary metabolites have quorum sensing inhibitor properties. This study aims to investigate an alternative treatment approach by utilizing lichen secondary metabolite evernic acid to reduce the expressions of Pseudomonas aeruginosa virulence factors by inhibiting quorum sensing. For this purpose, fluorescent monitor strains were utilized for quorum sensing inhibitor screens and quantitative reverse-transcriptase PCR analyses were conducted for comparison. Results indicate that evernic acid is capable of inhibiting Pseudomonas aeruginosa quorum sensing systems.
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Marmara University, Göztepe Campus, 34722, Istanbul, Turkey.
authors.forename: Barış
authors.initials: B
authors.lastname: Gökalsın
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Marmara University, Göztepe Campus, 34722, Istanbul, Turkey. csesal@marmara.edu.tr.
authors.forename: Nüzhet Cenk
authors.initials: NC
authors.lastname: Sesal
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006801:Humans; D062385:Hydroxybenzoates / Q000494:pharmacology*; D008011:Lichens / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics; D053038:Quorum Sensing / Q000187:drug effects*; D064210:Secondary Metabolism; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D062385:Hydroxybenzoates; D010936:Plant Extracts; D037521:Virulence Factors; C050847:evernic acid
keywords: Evernic acid; Lichen secondary metabolites; Pseudomonas aeruginosa; Quorum sensing