title: Enrichment and acclimation of an anaerobic mesophilic microorganism's inoculum for standardization of BMP assays.
abstract: Appropriate enrichment of anaerobic microorganism's consortium is crucial for accurate biochemical methane potential (BMP) assays. An alternative method to produce and maintain a mesophilic methanogenic inoculum was demonstrated. Three sources of inoculum were mixed and acclimated for 857days in order to reach steady conditions (pH=7.90±0.46; VS/TS>50%; VFA/alkalinity=0.16±0.04gAcetic Acid/ [Formula: see text] ). Biogas yield >80% was obtained after 70days of inoculum acclimation in comparison to standard cellulose (>600mLN/gVS). Methanogen community analysis based on 16S rDNA of the inoculum revealed Archaea concentration of 3×10(12) gene copies/g (Methanobacteriales 8×10(10); Methanomicrobiales 8×10(10); and Methanosarcinales 4×10(11) gene copies/g). The proposed method for development and maintenance of microorganism enrichment inoculum demonstrates consistent BMP data which is a requirement for dependable prediction of biogas production at field scale operations.
authors.affiliation: Embrapa Suínos e Aves, BR 153 km 110, 89700-000 Concórdia, SC, Brazil. Electronic address: ricardo.steinmetz@embrapa.br.
authors.forename: Ricardo Luis Radis
authors.initials: RLR
authors.lastname: Steinmetz
authors.affiliation: Universidade do Oeste de Santa Catarina - UNOESC, 89560-000 Videira, SC, Brazil. Electronic address: mmezzari@gmail.com.
authors.forename: Melissa Paola
authors.initials: MP
authors.lastname: Mezzari
authors.affiliation: Embrapa Suínos e Aves, BR 153 km 110, 89700-000 Concórdia, SC, Brazil. Electronic address: marcio.busi@embrapa.br.
authors.forename: Marcio Luis Busi
authors.initials: MLB
authors.lastname: da Silva
authors.affiliation: Embrapa Suínos e Aves, BR 153 km 110, 89700-000 Concórdia, SC, Brazil; Universidade Estadual do Oeste do Paraná - UNIOESTE/CCET/PGEAGRI, 85819110, Cascavel, PR, Brazil. Electronic address: airton.kunz@embrapa.br.
authors.forename: Airton
authors.initials: A
authors.lastname: Kunz
authors.affiliation: Universidade Estadual do Oeste do Paraná - UNIOESTE/CCET/PGEAGRI, 85819110, Cascavel, PR, Brazil. Electronic address: andrec.doamaral@gmail.com.
authors.forename: André Cestonaro
authors.initials: AC
authors.lastname: do Amaral
authors.affiliation: Universidade Estadual do Oeste do Paraná - UNIOESTE/CCET/PGEAGRI, 85819110, Cascavel, PR, Brazil. Electronic address: deisictapparo@gmail.com.
authors.forename: Deisi Cristina
authors.initials: DC
authors.lastname: Tápparo
authors.affiliation: Universidade Federal de Santa Catarina - UFSC, Departamento de Engenharia Química, 88034001 Florianópolis, SC, Brazil. Electronic address: hugo.moreira.soares@ufsc.br.
authors.forename: Hugo Moreira
authors.initials: HM
authors.lastname: Soares
mesh_terms: D000064:Acclimatization; D000468:Alkalies / Q000032:analysis; D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000378:metabolism*; D056804:Biofuels / Q000382:microbiology; D019149:Bioreactors / Q000382:microbiology; D002482:Cellulose / Q000737:chemistry; D004275:DNA, Ribosomal / Q000235:genetics; D005232:Fatty Acids, Volatile / Q000032:analysis; D008697:Methane / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012015:Reference Standards; D013696:Temperature
chemical_list: D000468:Alkalies; D056804:Biofuels; D004275:DNA, Ribosomal; D005232:Fatty Acids, Volatile; D002482:Cellulose; D008697:Methane; C109691:microcrystalline cellulose
keywords: Anaerobic digestion; Biogas; Methanogens; VDI 4630