title: The association between fructosamine-3 kinase 900C/G polymorphism, transferrin polymorphism and human herpesvirus-8 infection in diabetics living in South Kivu.
abstract: Prevalences of human herpesvirus-8 (HHV-8) infection and diabetes mellitus are very common in certain parts of Africa, containing iron-rich soils. We hypothesized that some genetic factors could have a link with susceptibility to HHV-8 infection. We focused on ferroportin Q248H mutation (rs11568350), transferrin (TF) polymorphism and fructosamine-3 kinase (FN3K) 900C/G polymorphism (rs1056534). The study population consisted of 210 type 2 diabetic adults and 125 healthy controls recruited in Bukavu (South Kivu). In the whole study population (diabetics+healthy controls), ferroportin Q248H mutation was detected in 47 subjects (14.0%) with 43 heterozygotes and 4 homozygotes. TF phenotype frequencies were 88.1% (CC), 10.4% (CD) and 1.5% (BC). Genotype frequencies of FN3K 900C/G polymorphism were respectively 9,3% (CC), 43.3% (GC) and 47.4% (GG). Prevalence of HHV8-infection in the study population was 77.3%. HHV-8 infection rate and HHV-8 IgG antibody titer were significantly higher in diabetics then in controls (p<0.0001). Significant differences were observed in HHV-8 infection rate and in HHV-8 IgG antibody titer according to FN3K rs1056534 (p<0.05 and p<0.05, respectively) and TF polymorphism (p<0.05 and p=0.005, respectively). No significant differences in HHV-8 infection rate and in HHV-8 IgG antibody titer were observed in the ferroportin Q248H mutation carriers (rs11568350) in comparison with ferroportin wild type. In a multiple regression analysis, FN3K rs1056534, TF polymorphism and presence of diabetes mellitus were predictors for HHV-8 infection. In contrast to these findings, ferroportin Q248H mutation (rs11568350) did not influence the susceptibility for an HHV-8 infection in sub-Saharan Africans.
authors.affiliation: Department of Internal Medicine, Catholic University of Bukavu, Bukavu, Democratic Republic of the Congo; Department of Clinical Chemistry, Ghent University Hospital, Ghent, Belgium.
authors.forename: Justin C
authors.initials: JC
authors.lastname: Cikomola
authors.affiliation: Laboratory of Molecular Diagnostics and Hematology, Ghent University Hospital, Ghent, Belgium.
authors.forename: Karl
authors.initials: K
authors.lastname: Vandepoele
authors.affiliation: Department of Internal Medicine, Catholic University of Bukavu, Bukavu, Democratic Republic of the Congo.
authors.forename: Philippe B
authors.initials: PB
authors.lastname: Katchunga
authors.affiliation: Department of Laboratory Medicine, Catholic University of Bukavu, Bukavu, Democratic Republic of the Congo.
authors.forename: Antoine S
authors.initials: AS
authors.lastname: Kishabongo
authors.affiliation: Department of Virology, Ghent University Hospital, Ghent, Belgium.
authors.forename: Elizaveta Y
authors.initials: EY
authors.lastname: Padalko
authors.affiliation: Department of Nephrology, Ghent University Hospital, Ghent, Belgium.
authors.forename: Marijn M
authors.initials: MM
authors.lastname: Speeckaert
authors.affiliation: Department of Clinical Chemistry, Ghent University Hospital, Ghent, Belgium. Electronic address: Joris.Delanghe@ugent.be.
authors.forename: Joris R
authors.initials: JR
authors.lastname: Delanghe
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D027682:Cation Transport Proteins / Q000235:genetics; D015023:Democratic Republic of the Congo / Q000453:epidemiology; D003924:Diabetes Mellitus, Type 2 / Q000150:complications; D005260:Female; D005838:Genotype; D006566:Herpesviridae Infections / Q000453:epidemiology* / Q000235:genetics / Q000821:virology; D019288:Herpesvirus 8, Human; D006801:Humans; D008297:Male; D008875:Middle Aged; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D011110:Polymorphism, Genetic; D015995:Prevalence; D012044:Regression Analysis; D014168:Transferrin / Q000235:genetics*; D000098742:Ferroportin
chemical_list: D027682:Cation Transport Proteins; D014168:Transferrin; D000098742:Ferroportin; D017853:Phosphotransferases (Alcohol Group Acceptor); C414347:fructosamine-3-kinase
keywords: Diabetes meliitus; Ferroportin Q248H mutation; Fructosamine-3 kinase 900C/G polymorphism; Human herpes virus-8; TF polymorphism