title: Bone Healing Properties of Autoclaved Autogenous Bone Grafts Incorporating Recombinant Human Bone Morphogenetic Protein-2 and Comparison of Two Delivery Systems in a Segmental Rabbit Radius Defect.
abstract: OBJECTIVE
  This study aims to validate the effect of autoclaved autogenous bone (AAB), incorporating Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2), on critical-sized, segmental radius defects in rabbits. Delivery systems using absorbable collagen sponge (ACS) and fibrin glue (FG) were also evaluated.
  
  
  METHODS
  Radius defects were made in 12 New Zealand white rabbits. After autoclaving, the resected bone was reinserted and fixed. The animals were classified into three groups: only AAB reinserted (group 1, control), and AAB and ErhBMP-2 inserted using an ACS (group 2) or FG (group 3) as a carrier. Animals were sacrificed six or 12 weeks after surgery. Specimens were evaluated using radiology and histology.
  
  
  RESULTS
  Micro-computed tomography images showed the best bony union in group 2 at six and 12 weeks after operation. Quantitative analysis showed all indices except trabecular thickness were the highest in group 2 and the lowest in group 1 at twelve weeks. Histologic results showed the greatest bony union between AAB and radial bone at twelve weeks, indicating the highest degree of engraftment.
  
  
  CONCLUSIONS
  ErhBMP-2 increases bony healing when applied on AAB graft sites. In addition, the ACS was reconfirmed as a useful delivery system for ErhBMP-2.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry; Department of Oral and Maxillofacial Surgery, Wonkwang University School of Dentistry.
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry; Department of Oral and Maxillofacial Surgery, National Health Insurance Service Ilsan Hospital.
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Kang
authors.affiliation: Department of Oral Biology, Yonsei University College of Dentistry.
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Kwon
authors.affiliation: Department of Oral Biology, Yonsei University College of Dentistry.
authors.forename: Sung-Won
authors.initials: SW
authors.lastname: Cho
authors.affiliation: Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry; Research Center for Orofacial Hard Tissue Regeneration, Yonsei University College of Dentistry.
authors.forename: Hyung Jun
authors.initials: HJ
authors.lastname: Kim
keywords: Autogenous autoclaved bone; BMP carrier; Bone morphogenetic protein 2; Critical bone defect; Rabbit radius