title: [Effects of Jianpi Jiedu Recipe on TCRVβCDR3 Spectratyping of Liver Cancer Rats with Pi Deficiency Syndrome].
abstract: OBJECTIVE
  To observe anti-cancer effects of Jianpi Jiedu Recipe (JJR) on liver cancer (LC) rats with Pi deficiency syndrome (PDS) and its relation with the third complementary-determining region gene spectratyping of TCRVβ-chain (TCRVβCDR3).
  
  
  METHODS
  Rats were divided into 8 groups according to random digit table, i.e., the blank control group (normal), the PDS group, the LC model group, the LC-PDS group, high, middle, and low dose JJR groups (75.00, 37.50, 18.75 g/kg, respectively by gastrogavage, once per day), the thymus pentapeptide group (5 mg/kg, intramuscular injection, twice per week), 8 in each group. Rats in the normal group were administered with physiological saline by gastrogavage once per day. PDS rat model was prepared by bitter-cold purgation. LC model was prepared by orthotopic transplantation method. Twenty gene subfamilies of TCRβCDR3 in the thymus, liver, and LC tissues were detected by Gene Scan.
  
  
  RESULTS
  High and middle dose JJR could postpone the growth of LC volume (P < 0.05), with equivalent liver index and thymus index to those of the normal group (P > 0.05). In thymus and liver tissue of the normal group, the number of clones (20 and 19), gene fragment number (220 and 113), Quasi-Gaussian distribution ratio of TCRVβCDR3 gene repertoire (100.0% and 42.1%), and fragment fluorescence peak area (6,539 ± 2,325 and 1,238 ± 439) were at the highest level among the 8 groups. TCRVβCDR3 expressions in thymus and liver tissue of high and middle dose JJR groups were approximate to those of the normal group. They were in the middle of the thymus pentapeptide group, the PDS group, the LC model group, and poorest in the LC-PDS group. TCRVβCDR3 in liver tissue expressed the best in the thymus pentapeptide group.
  
  
  CONCLUSIONS
  JJR might inhibit the growth of LC cells, and its mechanism might be related to enhancing TCRVβCDR3 spectratype expression.
authors.forename: Bao-guo
authors.initials: BG
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Ting
authors.initials: T
authors.lastname: Xiang
authors.forename: Ze-xiong
authors.initials: ZX
authors.lastname: Chen
authors.forename: Shi-jun
authors.initials: SJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D022801:Complementarity Determining Regions / Q000235:genetics*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D019674:Genes, T-Cell Receptor beta; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000235:genetics; D011897:Random Allocation; D051381:Rats
chemical_list: D022801:Complementarity Determining Regions; D004365:Drugs, Chinese Herbal; C000596357:jianpi jiedu