title: TALEN-Mediated Mutagenesis and Genome Editing.
abstract: Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifs for locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.
authors.affiliation: Department of Medicine, LKS Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Alvin C H
authors.initials: AC
authors.lastname: Ma
authors.affiliation: Mayo Clinic, Rochester, MN, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Mayo Clinic, Rochester, MN, USA.
authors.forename: Patrick R
authors.initials: PR
authors.lastname: Blackburn
authors.affiliation: Mayo Clinic, Rochester, MN, USA. ekker001@mail.med.umn.edu.
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Ekker
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems / Q000235:genetics; D053903:DNA Breaks, Double-Stranded; D000072669:Gene Editing / Q000379:methods*; D016296:Mutagenesis / Q000235:genetics*; D000069896:Transcription Activator-Like Effector Nucleases / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics
chemical_list: D000069896:Transcription Activator-Like Effector Nucleases
keywords: Customized nucleases; FusX; Genome editing; Golden Gate; TALEN; Zebrafish