title: Regulation of B-cell development and tolerance by different members of the miR-17∼92 family microRNAs.
abstract: The molecular mechanisms that regulate B-cell development and tolerance remain incompletely understood. In this study, we identify a critical role for the miR-17∼92 microRNA cluster in regulating B-cell central tolerance and demonstrate that these miRNAs control early B-cell development in a cell-intrinsic manner. While the cluster member miR-19 suppresses the expression of Pten and plays a key role in regulating B-cell tolerance, miR-17 controls early B-cell development through other molecular pathways. These findings demonstrate differential control of two closely linked B-cell developmental stages by different members of a single microRNA cluster through distinct molecular pathways.
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Maoyi
authors.initials: M
authors.lastname: Lai
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Alicia
authors.initials: A
authors.lastname: Gonzalez-Martin
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Anthony B
authors.identifier: 0000-0002-6485-5855
authors.initials: AB
authors.lastname: Cooper
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Hiroyo
authors.initials: H
authors.lastname: Oda
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.|Kellogg School of Science and Technology, The Scripps Research Institute, La Jolla, California 92037, USA.
authors.forename: Hyun Yong
authors.initials: HY
authors.lastname: Jin
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Jovan
authors.initials: J
authors.lastname: Shepherd
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Linling
authors.initials: L
authors.lastname: He
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: David
authors.initials: D
authors.lastname: Nemazee
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Changchun
authors.identifier: 0000-0001-8754-3208
authors.initials: C
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D007108:Immune Tolerance / Q000235:genetics*; D008213:Lymphocyte Activation / Q000235:genetics*; D008297:Male; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000502:physiology*; D023421:Models, Animal; D051059:PTEN Phosphohydrolase / Q000235:genetics*
chemical_list: C575895:MIRN17-92 microRNA, mouse; C561642:MIRN19 microRNA, mouse; D035683:MicroRNAs; C543614:Mirn17 microRNA, mouse; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse