title: Zerumbone induces gastric cancer cells apoptosis: Involving cyclophilin A.
abstract: Gastric cancer is one of the leading causes for cancer death. There is an urgent need to develop new therapeutic approaches targeting metastatic gastric cancer. It has been reported that zerumbone has the anti-cancer effects in various malignant cells. However, the effect and the mechanism of zerumbone on melanoma cells is still largely unknown. In the study, we determined the actions of zerumbone on the human gastric cancer cell line SGC-7901.We also observed the mechanism by which zerumbone induced gastric cancer cell apoptosis. Our data indicated that zerumbone significantly inhibited the growth of human gastric cancer cells in a dose-dependent manner and apoptosis was the main cause of decreased cell viability in zerumbone -treated cells. The treatment with zerumbone downregulated Cyp A and Bcl-2 levels, upregulated Bax levels, and caused Cytochrome c (Cyt-C) to release, activating Caspase-3. In summary, our study suggests that zerumbone mightinduced human gastric cancer cells apoptosis through down-regulating Cyp A and mitochondria-mediated pathways.
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuan 050011, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuan 050011, China. Electronic address: liyong195816@tom.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Divison of Medical Affairs, Hebei General Hospital, Shijiazhuang 050051, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Cui
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuan 050011, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuan 050011, China.
authors.forename: Bi-Bo
authors.initials: BB
authors.lastname: Tan
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuan 050011, China.
authors.forename: Zhi-Dong
authors.initials: ZD
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuan 050011, China.
authors.forename: Yv
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuan 050011, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jia
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D021984:Cyclophilin A / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D012717:Sesquiterpenes / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D012717:Sesquiterpenes; D051028:bcl-2-Associated X Protein; C403304:zerumbone; D045304:Cytochromes c; D053148:Caspase 3; D021984:Cyclophilin A
keywords: Apoptosis; Cyclophilin A; Gastric cancer; Mitochondria-mediated apoptosis; Zerumbone