title: Mitofusin 2 regulates the oocytes development and quality by modulating meiosis and mitochondrial function.
abstract: Mitofusin-2 (Mfn2), one of the mitochondrial dynamic proteins plays a key role in maintaining the integrity of mitochondrial morphology and function. However, it is unknown if Mfn2 influences the quality of oocytes in the process of development by modulating mitochondrial function in vitro. In this study, immature oocytes were transfected with Mfn2-siRNA for 16 h. We found that the expression level of the Mfn2 gene was significantly lower than those of the control group. The rates of maturation and fertility were also found to have declined. Moreover, mitochondrial structure and function, especially the morphogenesis of spindles, were observed as abnormal during meiosis. Thus, the above findings indicate that down-regulation of Mfn2 may have an impact on the maturation and fertilization of immature oocytes in vitro by modulating meiosis and mitochondrial function.
authors.affiliation: Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Kang
authors.affiliation: Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Lingjuan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Wenpei
authors.initials: W
authors.lastname: Xiang
mesh_terms: D000818:Animals; D005260:Female; D020558:GTP Phosphohydrolases / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008540:Meiosis; D051379:Mice; D008813:Mice, Inbred ICR; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*
chemical_list: D020558:GTP Phosphohydrolases; C488751:Mfn2 protein, mouse