title: Biomolecular proteomics discloses ATP synthase as the main target of the natural glycoside deglucoruscin.
abstract: Extracts of Ruscus aculeatus are a rich source of bioactive steroidal glycosides, such as ruscogenins which are reported to act against chronic venous disorders. Nowadays, several preparations of its roots, commonly used in traditional medicine, are on the market as food supplements for health care and maintenance. Although spirostanol deglucoruscin is one of the main metabolites in these extracts, literature reports about its pharmacological profile are scarce. In this paper, a multi-disciplinary approach, based on chemical proteomics, molecular modelling and bio-organic assays, has been used to disclose the whole interactome of deglucoruscin and the F0-F1 ATP synthase complex has been found as its main target.
authors.affiliation: Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084, Fisciano, Italy. mcmonti@unisa.it.
authors.forename: Federica
authors.initials: F
authors.lastname: Del Gaudio
authors.forename: Carmen
authors.initials: C
authors.lastname: Festa
authors.forename: Matteo
authors.initials: M
authors.lastname: Mozzicafreddo
authors.forename: Michele
authors.initials: M
authors.lastname: Vasaturo
authors.forename: Agostino
authors.initials: A
authors.lastname: Casapullo
authors.forename: Simona
authors.initials: S
authors.lastname: De Marino
authors.forename: Raffaele
authors.initials: R
authors.lastname: Riccio
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Monti
mesh_terms: D001688:Biological Products / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002846:Chromatography, Affinity; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D013058:Mass Spectrometry; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D040901:Proteomics / Q000379:methods; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D031443:Ruscus / Q000737:chemistry
chemical_list: D001688:Biological Products; D006027:Glycosides; D010936:Plant Extracts; D006180:Proton-Translocating ATPases