title: A Bordetella pertussis proteoliposome induces protection in mice without affecting the immunogenicity of diphtheria and tetanus toxoids in a trivalent formulation.
abstract: In this study, a formulation of Bordetella pertussis proteoliposome (PLBp), diphtheria, and tetanus toxoids and alum (DT-PLBp) was evaluated as a trivalent vaccine candidate in BALB/c mice. Vaccine-induced protection was estimated using the intranasal challenge for pertussis and enzyme-linked immunosorbent assay fvto assess serological responses for diphtheria or tetanus. Both, diphtheria-tetanus-whole cell pertussis (DTP) and diphtheria-tetanus vaccines (DT) were used as controls. Animals immunized with DT-PLBp, PLBp alone, and DTP showed total reduction of CFU in lungs 7 days after intranasal challenge. Likewise, formulations DT-PLBp, DTP, and DT elicited antibody levels ≥2 IU/mL against tetanus and diphtheria, considered protective when neutralization tests are used. Overall, results showed that combination of PLBp with tetanus and diphtheria toxoids did not affect the immunogenicity of each antigen alone.
authors.affiliation: Finlay Institute, Havana, Cuba.
authors.forename: Sonsire Fernández
authors.identifier: 0000-0001-5329-5971
authors.initials: SF
authors.lastname: Castillo
authors.affiliation: Finlay Institute, Havana, Cuba.
authors.forename: Mario Landys
authors.identifier: 0000-0002-6991-7007
authors.initials: ML
authors.lastname: Chovel
authors.affiliation: Finlay Institute, Havana, Cuba.
authors.forename: Niurka Gutiérrez
authors.identifier: 0000-0002-3896-9648
authors.initials: NG
authors.lastname: Hernández
authors.affiliation: Finlay Institute, Havana, Cuba.
authors.forename: Lorena Corcho
authors.identifier: 0000-0002-0084-281X
authors.initials: LC
authors.lastname: González
authors.affiliation: Medical Faculty and Institute of Basic and Preclinical Science "Victoria de Girón", University of Medical Sciences of Havana, Havana, Cuba.
authors.forename: Amaya
authors.identifier: 0000-0002-5049-4024
authors.initials: A
authors.lastname: Blanco
authors.affiliation: Finlay Institute, Havana, Cuba.
authors.forename: Daily Serrano
authors.identifier: 0000-0001-9233-2376
authors.initials: DS
authors.lastname: Hernández
authors.affiliation: Finlay Institute, Havana, Cuba.
authors.forename: Mildrey Fariñas
authors.identifier: 0000-0001-6530-9904
authors.initials: MF
authors.lastname: Medina
authors.affiliation: Finlay Institute, Havana, Cuba.
authors.forename: Maydelis Álvarez
authors.identifier: 0000-0002-7507-6013
authors.initials: MÁ
authors.lastname: Tito
authors.affiliation: Finlay Institute, Havana, Cuba.
authors.forename: José Luis Pérez
authors.identifier: 0000-0001-8325-2120
authors.initials: JL
authors.lastname: Quiñoy
keywords: Bordetella pertussis; Proteoliposome; Vaccines; Whooping cough