title: Physicochemical and in vitro biocompatibility of films combining reconstituted bacterial cellulose with arabinogalactan and xyloglucan.
abstract: Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process. Arabinogalactan (AG) extracted from Pereskia aculeata leaves and/or a xyloglucan (GHXG) from Guibourtia hymenifolia seeds were incorporating into the RBC at various compositions, and new films were created using the same process. Biocomposite properties were evaluated by scanning electron microscopy, contact angle (CA), and X-ray diffraction measurements. The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated. RBC films with 20-30% GHXG replacement improved film stability and the inclusion of HD increased microfiber aggregation and reduced porous regions. Changes in the hydrophilic characteristics were also observed and owing to the adhesion effect the inclusion of HD on RBC led to a statistically significant effect of the mechanical properties of films. The RBC/AG films supported L929 adhesion similar to that observed for commercial bacterial cellulose, indicating their potential use for biomedical applications.
authors.affiliation: BIOPOL, Department of Chemistry, Universidade Federal do Paraná-UFPR, Centro Politécnico, P.O. Box 19081, PR, Brazil; Chemistry Undergraduate Program, School of Education and Humanities, Pontifícia Universidade Católica do Paraná - PUCPR, Curitiba, PR, Brazil. Electronic address: neoli.lucyszyn@pucpr.br.
authors.forename: Neoli
authors.initials: N
authors.lastname: Lucyszyn
authors.affiliation: Yasuyoshi Hayashi Microbiology Laboratory, Department of Basic Pathology, Universidade Federal do Paraná - UFPR, P.O. Box 19031, Curitiba, PR, Brazil.
authors.forename: Lucy
authors.initials: L
authors.lastname: Ono
authors.affiliation: LITS, Physics Department, Universidade Federal do Paraná-UFPR, P.O. Box 19044, Curitiba, PR, Brazil.
authors.forename: Adriana Freire
authors.initials: AF
authors.lastname: Lubambo
authors.affiliation: BIOPOL, Department of Chemistry, Universidade Federal do Paraná-UFPR, Centro Politécnico, P.O. Box 19081, PR, Brazil.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Woehl
authors.affiliation: BIOPOL, Department of Chemistry, Universidade Federal do Paraná-UFPR, Centro Politécnico, P.O. Box 19081, PR, Brazil.
authors.forename: Camila V
authors.initials: CV
authors.lastname: Sens
authors.affiliation: BIOPOL, Department of Chemistry, Universidade Federal do Paraná-UFPR, Centro Politécnico, P.O. Box 19081, PR, Brazil; Chemistry Undergraduate Program, School of Education and Humanities, Pontifícia Universidade Católica do Paraná - PUCPR, Curitiba, PR, Brazil.
authors.forename: Clayton F
authors.initials: CF
authors.lastname: de Souza
authors.affiliation: BIOPOL, Department of Chemistry, Universidade Federal do Paraná-UFPR, Centro Politécnico, P.O. Box 19081, PR, Brazil.
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: Sierakowski
mesh_terms: D000818:Animals; D001458:Bandages / Q000382:microbiology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002482:Cellulose / Q000737:chemistry* / Q000494:pharmacology*; D055598:Chemical Phenomena; D005685:Galactans / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry*; D008567:Membranes, Artificial; D051379:Mice; D014990:Xylans / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D005685:Galactans; D005936:Glucans; D008567:Membranes, Artificial; D014990:Xylans; C029353:xyloglucan; D002482:Cellulose; C005653:arabinogalactan
keywords: Arabinogalactan; Bacterial cellulose; Cell adhesion; Films; Xyloglucan