title: Rapid protein immobilization for thin film continuous flow biocatalysis.
abstract: A versatile enzyme immobilization strategy for thin film continuous flow processing is reported. Here, non-covalent and glutaraldehyde bioconjugation are used to immobilize enzymes on the surfaces of borosilicate reactors. This approach requires only ng of protein per reactor tube, with the stock protein solution readily recycled to sequentially coat >10 reactors. Confining reagents to thin films during immobilization reduced the amount of protein, piranha-cleaning solution, and other reagents by ∼96%. Through this technique, there was no loss of catalytic activity over 10 h processing. The results reported here combines the benefits of thin film flow processing with the mild conditions of biocatalysis.
authors.affiliation: Departments of Chemistry, Molecular Biology and Biochemistry, University of California, Irvine, California 92697-2025, USA. gweiss@uci.edu and Centre for NanoScale Science and Technology, School of Chemical and Physical Sciences, Flinders University, Bedford Park, Adelaide, South Australia 5001, Australia. colin.raston@flinders.edu.au.
authors.forename: Joshua
authors.initials: J
authors.lastname: Britton
authors.affiliation: Centre for NanoScale Science and Technology, School of Chemical and Physical Sciences, Flinders University, Bedford Park, Adelaide, South Australia 5001, Australia. colin.raston@flinders.edu.au.
authors.forename: Colin L
authors.initials: CL
authors.lastname: Raston
authors.affiliation: Departments of Chemistry, Molecular Biology and Biochemistry, University of California, Irvine, California 92697-2025, USA. gweiss@uci.edu.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Weiss
mesh_terms: D055162:Biocatalysis; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004800:Enzymes, Immobilized / Q000032:analysis* / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D004800:Enzymes, Immobilized