title: A Search for the Optimum Selenium Source to Obtain Mushroom-Derived Chemopreventive Preparations.
abstract: The objective of this research was to test whether selenium-yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides. Culture media were enriched in selenium to a concentration of 20 µg/mL. Selenium was added to the medium either in the form of sodium selenite or in form of Se-yeast (Sel-Plex; Alltech Inc., Lexington, KY). The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides were determined using atomic absorption spectroscopy. We found that selenium accumulated more efficiently in cultures enriched with Se-yeast. A higher concentration of selenium was also found in the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media. With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
authors.affiliation: Department of Food and Industrial Microbiology, Faculty of Agriculture, University of Belgrade, Belgrade, Serbia.
authors.forename: Milena
authors.initials: M
authors.lastname: Savic
authors.affiliation: Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Marzenna
authors.initials: M
authors.lastname: Klimaszewska
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bamburowicz-Klimkowska
authors.affiliation: Department of Drug Analysis, Medical University of Warsaw, Warsaw, Poland; National Medicines Institute, Department of Pharmaceutical Chemistry, Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Suchocki
authors.affiliation: Department of Food and Industrial Microbiology, Faculty of Agriculture, University of Belgrade, Belgrade, Serbia.
authors.forename: Miomir
authors.initials: M
authors.lastname: Niksic
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Szutowski
authors.affiliation: Department of Drug Analysis, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Wroczynski
authors.affiliation: Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Turlo
mesh_terms: D002473:Cell Wall / Q000737:chemistry; D003470:Culture Media; D008401:Gas Chromatography-Mass Spectrometry; D025282:Mycelium / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D012643:Selenium / Q000302:isolation & purification* / Q000378:metabolism; D020942:Shiitake Mushrooms / Q000737:chemistry* / Q000378:metabolism; D018038:Sodium Selenite / Q000378:metabolism*; D013054:Spectrophotometry, Atomic
chemical_list: D003470:Culture Media; D011134:Polysaccharides; D012643:Selenium; D018038:Sodium Selenite