title: Jabuticaba (Myrciaria cauliflora) Seeds: Chemical Characterization and Extraction of Antioxidant and Antimicrobial Compounds.
abstract: This study was aimed to assess the effect of time and temperature on the extraction of antioxidant compounds from jabuticaba seeds (Myrciaria cauliflora cv. Sabará), to optimize the solvent proportion (water, ethyl alcohol, and propanone), and to characterize the extract according to the chemical composition, antioxidant, and antimicrobial properties. Proximal composition, total phenolic content (TPC), antioxidant, and antimicrobial activities were analyzed. The optimized solvent ratio of 60% water and 40% propanone provided a mean TPC of 8.65 g GAE/100 g seeds and the antioxidant activity toward 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 82.79% ± 0.50%. Time and temperature parameters did not influence the yield of TPC. The gross seed extract was partially purified and both exhibited a high antioxidant activity and antimicrobial potential toward Gram-positive and Gram-negative bacteria. The purified jabuticaba seed lyophilized extract contained a higher (P < 0.05) TPC, o-diphenols, flavonols, and antioxidant activity measured by the DPPH assay and total reducing capacity as compared to the gross lyophilized extract. Electrospray ionization coupled with tandem mass spectrometry (ESI-MS/MS) data showed the presence of ellagitannins and ellagic acid in the extracts, which are probably the responsible for the antimicrobial and antioxidant activities.
authors.affiliation: Dept. of Chemistry, State Univ. of Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil.
authors.forename: Ana Carolina Mendes
authors.initials: AC
authors.lastname: Hacke
authors.affiliation: Graduation Program in Food Science and Technology, State Univ. of Ponta Grossa, Ponta Grossa, PR, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Granato
authors.affiliation: Graduation Program in Food Science and Technology, State Univ. of Ponta Grossa, Ponta Grossa, PR, Brazil.
authors.forename: Laércio Galvão
authors.initials: LG
authors.lastname: Maciel
authors.affiliation: Dept. of Chemistry, State Univ. of Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil.
authors.forename: Patrícia Los
authors.initials: PL
authors.lastname: Weinert
authors.affiliation: Dept. of Food Science, Univ. of Campinas, Campinas, SP, Brazil.
authors.forename: Leonardo do
authors.initials: Ld
authors.lastname: Prado-Silva
authors.affiliation: Dept. of Food Science, Univ. of Campinas, Campinas, SP, Brazil.
authors.forename: Verônica Ortiz
authors.initials: VO
authors.lastname: Alvarenga
authors.affiliation: Dept. of Food Science, Univ. of Campinas, Campinas, SP, Brazil.
authors.forename: Anderson
authors.initials: A
authors.lastname: de Souza Sant'Ana
authors.affiliation: ThoMSon Mass Spectrometry Laboratory, Inst. of Chemistry, Univ. of Campinas, Campinas, SP, Brazil.
authors.forename: Giovana Anceski
authors.initials: GA
authors.lastname: Bataglion
authors.affiliation: ThoMSon Mass Spectrometry Laboratory, Inst. of Chemistry, Univ. of Campinas, Campinas, SP, Brazil.
authors.forename: Marcos Nogueira
authors.initials: MN
authors.lastname: Eberlin
authors.affiliation: Dept. of Chemistry, State Univ. of Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil. ndrosso@uepg.br.|Graduation Program in Food Science and Technology, State Univ. of Ponta Grossa, Ponta Grossa, PR, Brazil. ndrosso@uepg.br.
authors.forename: Neiva Deliberali
authors.initials: ND
authors.lastname: Rosso
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D004610:Ellagic Acid / Q000032:analysis / Q000494:pharmacology; D044948:Flavonols / Q000737:chemistry / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D047348:Hydrolyzable Tannins / Q000032:analysis / Q000494:pharmacology; D027822:Myrtaceae / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D044948:Flavonols; D047348:Hydrolyzable Tannins; D010636:Phenols; D010936:Plant Extracts; D004610:Ellagic Acid
keywords: ESI-MS/MS; antimicrobial activity; phenolic compounds; simplex-centroid design; solvent effect