title: Separation of Peptides on HALO 2-Micron Particles.
abstract: Reversed-phase high-performance liquid chromatography (RP-HPLC) is of fundamental importance to the isolation and separation of peptides, proteins, and other biomolecules. Hence, there is a continuing high demand for the development of RP-HPLC stationary-phase materials with enhanced separation efficiency. HALO packing materials began the revolution in "core-shell" technology with the advantages of faster separations, higher resolution and peak capacity, high temperature stability, and rugged reliable performance compared to traditional HPLC and UHPLC. These materials are characterized by a solid core surrounded by a thin layer of porous material, and represent a technology for the future with continuing refinements. Such refinements are aided via the use of designed synthetic peptide standards during stationary-phase development. Concomitantly, such standards also enable the researcher to monitor RP-HPLC column performance and develop optimized separation protocols for peptides from a wide array of sources. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Colorado, School of Medicine, Aurora, Colorado.
authors.forename: Colin T
authors.initials: CT
authors.lastname: Mant
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Colorado, School of Medicine, Aurora, Colorado.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Hodges
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D010316:Particle Size; D010455:Peptides / Q000302:isolation & purification*; D016062:Porosity; D011506:Proteins / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D014269:Trifluoroacetic Acid / Q000737:chemistry
chemical_list: D010455:Peptides; D011506:Proteins; D012822:Silicon Dioxide; D014269:Trifluoroacetic Acid
keywords: 2.0 micron particles; anionic ion-pairing reagent; core-shell; fused-core particles; reversed-phase HPLC; separation of peptides; solid core; trifluoroacetic acid (TFA)