title: Demonstration of d-Octaarginine-Linked Polymers as Promising Adjuvants for Mucosal Vaccination through Influenza Virus Challenge.
abstract: Mucosal vaccination is one of the most effective ways to reduce the risk of pandemics as a result of incorrect prediction of epidemic strains of influenza viruses or virus mutation. However, adjuvants and antigen carriers with potent immunostimulatory activities are a prerequisite for significant induction of mucosal immunity because most antigens are poorly immunogenic when solely applied to the mucosa. Our previous studies demonstrated that poly(N-vinylacetamide-co-acrylic acid) bearing d-octaarginine induced the secretion of antigen-specific immunoglobulin A (IgA) on the mucosa when nasally administered with virus antigens and that intranasal IgA reacts to viral strains other than the one used for immunization. Therefore, the present study evaluated capabilities of secreted IgA for protection against virus infection. When mice were inoculated with a mixture of inactivated H1N1 A/Puerto Rico/8/34 influenza viruses and d-octaarginine-linked polymers, antigen-specific secreted IgA was induced on the nasal mucosa. Immunized mice were completely protected from virus infection of the inoculated strain. To the contrary, mice nasally inoculated with inactivated viruses alone were infected with the homologous viruses presumably because of insignificant induction of secreted IgA. Results demonstrated that our polymer would be a promising adjuvant for mucosal vaccination.
authors.affiliation: Faculty of Pharmaceutical Sciences, Setsunan University , 45-1, Nagaotoge-cho, Hirakata, Osaka 573-0101, Japan.|Life Science Materials Laboratory, ADEKA Co. , 7-2-34 Higashiogu, Arakawa-ku, Tokyo 116-8553, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Miyata
authors.affiliation: Faculty of Pharmaceutical Sciences, Setsunan University , 45-1, Nagaotoge-cho, Hirakata, Osaka 573-0101, Japan.
authors.forename: Kohta
authors.initials: K
authors.lastname: Mohri
authors.affiliation: Faculty of Pharmaceutical Sciences, Setsunan University , 45-1, Nagaotoge-cho, Hirakata, Osaka 573-0101, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Egawa
authors.affiliation: Faculty of Pharmaceutical Sciences, Setsunan University , 45-1, Nagaotoge-cho, Hirakata, Osaka 573-0101, Japan.
authors.forename: Rikito
authors.initials: R
authors.lastname: Endo
authors.affiliation: Life Science Materials Laboratory, ADEKA Co. , 7-2-34 Higashiogu, Arakawa-ku, Tokyo 116-8553, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Morimoto
authors.affiliation: Life Science Materials Laboratory, ADEKA Co. , 7-2-34 Higashiogu, Arakawa-ku, Tokyo 116-8553, Japan.
authors.forename: Kyohei
authors.initials: K
authors.lastname: Ochiai
authors.affiliation: Life Science Materials Laboratory, ADEKA Co. , 7-2-34 Higashiogu, Arakawa-ku, Tokyo 116-8553, Japan.
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Hiwatari
authors.affiliation: Life Science Materials Laboratory, ADEKA Co. , 7-2-34 Higashiogu, Arakawa-ku, Tokyo 116-8553, Japan.
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Tsubaki
authors.affiliation: Life Science Materials Laboratory, ADEKA Co. , 7-2-34 Higashiogu, Arakawa-ku, Tokyo 116-8553, Japan.
authors.forename: Etsuo
authors.initials: E
authors.lastname: Tobita
authors.affiliation: School of Medicine, Miyazaki University , Kihara 5200 Kiyotake-cho, Miyazaki, Miyazaki 889-1692, Japan.
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Uto
authors.affiliation: Graduate School of Medical and Dental Sciences, Kagoshima University , 8-35-1, Sakuragaoka, Kagoshima, Kagoshima 890-8544, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Baba
authors.affiliation: Faculty of Pharmaceutical Sciences, Setsunan University , 45-1, Nagaotoge-cho, Hirakata, Osaka 573-0101, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakuma
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D005260:Female; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000276:immunology*; D009842:Oligopeptides / Q000737:chemistry* / Q000276:immunology; D011108:Polymers / Q000737:chemistry*; D014611:Vaccination
chemical_list: D000180:Acrylic Resins; D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D009842:Oligopeptides; D011108:Polymers; C448619:octaarginine