title: Gold nanoparticles labeling with hybridization chain reaction amplification strategy for the sensitive detection of HepG2 cells by inductively coupled plasma mass spectrometry.
abstract: Sensitive detection of circulating tumor cells (CTCs) is of great significance in the early detection of cancer and cancer metastasis. This work reported an efficient, specific, and sensitive immunoassay protocol for detection of tumor cells by using inductively coupled plasma mass spectrometry (ICP-MS) with gold nanoparticles (AuNPs) labeling and hybridization chain reaction (HCR) amplification. In the established approach, antibodies against epithelial cell adhesion molecule (anti-EpCAM) conjugated magnetic beads (MBs) were used for selective capture of tumor cells from peripheral blood, aptamer was applied for the recognition of captured tumor cells, and AuNPs labeled DNA concatamer was used as the signal probe for tumor cell labeling and ICP-MS detection. Due to the dual amplification effect of AuNPs and HCR, the limit of detection of this ICP-MS based method for HepG2 cells was as low as 15 cells, and the linear range was 40-8000 cells with the relative standard deviation for seven replicate detections of 200 HepG2 cells was 8.7%. Furthermore, the applicability of the method for the analysis of peripheral blood samples was demonstrated by the spiking tests. The established method was highly specific and sensitive for the detection of HepG2 cells, and has a good application potential in clinical diagnosis.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072 PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072 PR China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072 PR China.
authors.forename: Man
authors.initials: M
authors.lastname: He
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072 PR China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072 PR China. Electronic address: binhu@whu.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics; D002452:Cell Count / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013054:Spectrophotometry, Atomic / Q000379:methods*; D013194:Staining and Labeling
chemical_list: D052157:Aptamers, Nucleotide; D006046:Gold
keywords: Aptamer; Circulating tumor cells; Gold nanoparticles; Hybridization chain reaction; Inductively coupled plasma mass spectrometry