title: Histologic characterization of regenerated tissues after pulp revascularization of immature dog teeth with apical periodontitis using tri-antibiotic paste and platelet-rich plasma.
abstract: BACKGROUND
  This study evaluates histologically the efficacy of 4 revascularization protocols in necrotic-infected immature dog teeth with apical periodontitis (AP).
  
  
  METHODS
  Forty double-rooted immature premolar teeth from 4 female Beagle dogs aged 5 months were used. Four teeth were left untouched as negative controls; the other 36 teeth were infected to develop pulp necrosis and AP. Four teeth were left untreated and assigned to the positive control group. The last 28 teeth were randomly assigned into four experimental groups of 8 teeth, each one treated with a different treatment protocol: A1, sodium hypochlorite (SH)+blood clot (BC); A2, SH+platelet-rich plasma (PRP); B1, SH+modified tri-antibiotic paste (mTAP)+BC; B2, SH+mTAP+PRP. The animals were sacrificed, histologic sections were prepared and three parameters were assessed: (1) presence or absence of new hard tissue on the internal root dentinal walls, (2) presence or absence of continued apical closure, and (3) presence or absence of vital tissue within the canal space.
  
  
  RESULTS
  Significant differences (p<0.05) between the four experimental groups were evident in the percentage of teeth showing histological apical closure (34.5%) and vital tissue within the canal space (68.8%). Group B2 showed the maximal improvement in the three variables assessed (p<0.05). Group A1 showed the minimum percentages in the three parameters assessed (p<0.05).
  
  
  CONCLUSIONS
  These results suggest that an intracanal dressing of mTAP, and the use of PRP as scaffold, improves the success rate of the revascularization procedure.
authors.affiliation: Department of Stomatology, School of Dentistry, University of Sevilla, C/Avicena s/n, 41009 Sevilla, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Stambolsky
authors.affiliation: Department of Stomatology, School of Dentistry, University of Sevilla, C/Avicena s/n, 41009 Sevilla, Spain.
authors.forename: Soledad
authors.initials: S
authors.lastname: Rodríguez-Benítez
authors.affiliation: Department of Stomatology, School of Dentistry, Virgen Macarena University Hospital, Andalusian Health Service, University of Sevilla, C/Avicena s/n, 41009 Sevilla, Spain.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Gutiérrez-Pérez
authors.affiliation: Department of Stomatology, School of Dentistry, University of Sevilla, C/Avicena s/n, 41009 Sevilla, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Torres-Lagares
authors.affiliation: Department of Stomatology, School of Dentistry, University of Sevilla, C/Avicena s/n, 41009 Sevilla, Spain.
authors.forename: Jenifer
authors.initials: J
authors.lastname: Martín-González
authors.affiliation: Department of Stomatology, School of Dentistry, University of Sevilla, C/Avicena s/n, 41009 Sevilla, Spain. Electronic address: segurajj@us.es.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Segura-Egea
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D001641:Bicuspid; D017610:Calcium Compounds / Q000494:pharmacology; D020682:Cefixime / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D003131:Combined Modality Therapy; D003782:Dental Pulp / Q000098:blood supply*; D003790:Dental Pulp Necrosis / Q000000981:diagnostic imaging / Q000628:therapy*; D004285:Dogs; D004338:Drug Combinations; D005260:Female; D008795:Metronidazole / Q000494:pharmacology; D010087:Oxides / Q000494:pharmacology; D010485:Periapical Periodontitis / Q000000981:diagnostic imaging / Q000628:therapy*; D053657:Platelet-Rich Plasma; D011897:Random Allocation; D012388:Root Canal Irrigants / Q000494:pharmacology; D012390:Root Canal Therapy / Q000379:methods*; D017640:Silicates / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012388:Root Canal Irrigants; D017640:Silicates; C086631:mineral trioxide aggregate; D008795:Metronidazole; D002939:Ciprofloxacin; D020682:Cefixime; D012973:Sodium Hypochlorite
keywords: Apical negative pressure irrigation; Immature tooth; Mineral trioxide aggregate; Platelet-rich plasma; Pulp regeneration; Pulp revascularization; Scaffold; Tri-antibiotic paste