title: Surviving nebulization-induced stress: dexamethasone in pH-sensitive archaeosomes.
abstract: To increase the subcellular delivery of dexamethasone phosphate (DP) and stability to nebulization stress, pH-sensitive nanoliposomes (LpH) exhibiting archaeolipids, acting as ligands for scavenger receptors (pH-sensitive archaeosomes [ApH]), were prepared.
  
  
  
  The anti-inflammatory effect of 0.18 mg DP/mg total lipid, 100-150 nm DP-containing ApH (dioleylphosphatidylethanolamine: Halorubrum tebenquichense total polar archaeolipids:cholesteryl hemisuccinate 4.2:2.8:3 w:w) was tested on different cell lines. Size and HPTS retention of ApH and conventional LpH (dioleylphosphatidylethanolamine:cholesteryl hemisuccinate 7:3 w:w) before and after nebulization were determined.
  
  
  
  DP-ApH suppressed IL-6 and TNF-α on phagocytic cells. Nebulized after 6-month storage, LpH increased size and completely lost its HPTS while ApH3 conserved size and polydispersity, fully retaining its original HPTS content.
authors.affiliation: Nanomedicine Research Program, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes. Roque Saenz Peña 352, Bernal B1876BXD, Argentina.
authors.forename: Maria Julia
authors.initials: MJ
authors.lastname: Altube
authors.affiliation: Nanomedicine Research Program, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes. Roque Saenz Peña 352, Bernal B1876BXD, Argentina.
authors.forename: Solange Mailen
authors.initials: SM
authors.lastname: Selzer
authors.affiliation: Brazilian Nanotechnology National Laboratory, CNPEM, Caixa Postal 6192, CEP 13.083-970, Campinas, São Paulo, Brazil.
authors.forename: Marcelo Alexandre
authors.initials: MA
authors.lastname: de Farias
authors.affiliation: Brazilian Nanotechnology National Laboratory, CNPEM, Caixa Postal 6192, CEP 13.083-970, Campinas, São Paulo, Brazil.
authors.forename: Rodrigo Villares
authors.initials: RV
authors.lastname: Portugal
authors.affiliation: Nanomedicine Research Program, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes. Roque Saenz Peña 352, Bernal B1876BXD, Argentina.
authors.forename: Maria Jose
authors.initials: MJ
authors.lastname: Morilla
authors.affiliation: Nanomedicine Research Program, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes. Roque Saenz Peña 352, Bernal B1876BXD, Argentina.
authors.forename: Eder Lilia
authors.initials: EL
authors.lastname: Romero
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002460:Cell Line; D003692:Delayed-Action Preparations / Q000737:chemistry*; D003907:Dexamethasone / Q000008:administration & dosage* / Q000494:pharmacology; D004355:Drug Stability; D055352:Halorubrum / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015850:Interleukin-6 / Q000276:immunology; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008807:Mice, Inbred BALB C; D009330:Nebulizers and Vaporizers; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000276:immunology; D000093242:Interleukin-6 Inhibitors
chemical_list: D000893:Anti-Inflammatory Agents; D003692:Delayed-Action Preparations; D003907:Dexamethasone; D015850:Interleukin-6; D008055:Lipids; D008081:Liposomes; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors
keywords: lung; macrophages targeting; nebulization; subcellular delivery