title: Plasma MiRNA alterations between NSCLC patients harboring Del19 and L858R EGFR mutations.
abstract: Based on recognition of driver mutations, treatment paradigm for non-small-cell lung cancer (NSCLC) patients has been shifted. However, recently exon 19 deletion mutation (del19) of epidermal growth factor receptor (EGFR) clearly shows better clinical benefit over single-point substitution mutation L858R in exon 21 (L858R). The aim of this study was to investigate the difference by analyzing the expression of plasma microRNAs (miRNAs) of NSCLC patients with EGFR mutation del19 or L858R. MiRNA microarray of plasma from patients' blood identified 79 mapped, network-eligible miRNAs (fold > 5), of which 76 were up regulated and 3 were down regulated. Genetic network was performed with Ingenuity Pathway Analysis (IPA). Among analysis, MYC, Argonaute2 (AGO2), Y-box binding protein 1 (YBX1), cyclin E1 (CCNE1) were involved in organismal abnormalities and cancer. Our findings provide information on the epigenetic signature of the two major sensitive mutations among NSCLC and add to the understanding of mechanisms underlying the different outcomes.
authors.affiliation: Xiamen Cancer Hospital, The First Affiliated Hospital of Xiamen University, 361003, Fujian, China.
authors.forename: Yihan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Reproduction Center, The Second Affiliated Hospital of Kunming Medical University 650101, Yunnan, China.
authors.forename: Peiqi
authors.initials: P
authors.lastname: Xu
authors.affiliation: Xiamen Cancer Hospital, The First Affiliated Hospital of Xiamen University, 361003, Fujian, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Mi
authors.affiliation: Xiamen Cancer Hospital, The First Affiliated Hospital of Xiamen University, 361003, Fujian, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Wang
authors.affiliation: Xiamen Cancer Hospital, The First Affiliated Hospital of Xiamen University, 361003, Fujian, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Pan
authors.affiliation: Xiamen Cancer Hospital, The First Affiliated Hospital of Xiamen University, 361003, Fujian, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Wu
authors.affiliation: Xiamen Cancer Hospital, The First Affiliated Hospital of Xiamen University, 361003, Fujian, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: He
authors.affiliation: Xiamen Cancer Hospital, The First Affiliated Hospital of Xiamen University, 361003, Fujian, China.
authors.forename: Hongming
authors.initials: H
authors.lastname: Liu
authors.affiliation: Xiamen Cancer Hospital, The First Affiliated Hospital of Xiamen University, 361003, Fujian, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Tang
authors.affiliation: Xiamen Cancer Hospital, The First Affiliated Hospital of Xiamen University, 361003, Fujian, China.
authors.forename: Hanxiang
authors.initials: H
authors.lastname: An
mesh_terms: D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020125:Mutation, Missense; D017384:Sequence Deletion
chemical_list: D035683:MicroRNAs; D066246:ErbB Receptors
keywords: EGFR; NSCLC; circulating miRNA; microarray; response