title: Structural characterization and functional properties of antihypertensive Cymodocea nodosa sulfated polysaccharide.
abstract: A sulfated polysaccharide was successfully isolated from Cymodocea nodosa (CNSP). This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide. The CNSP consisted mainly of sulfate (23.17%), total sugars (54.90%), galactose (44.89%), mannose (17.30%), arabinose (12.05%), xylose (9.18%), maltose (1.07%) and uronic acid (11.03%) with low water activity (0.49). CNSP had an XRD pattern that was typical for a semi-crystalline polymer with homogeneous structure. It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL). Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
authors.affiliation: Laboratory of Plant Biotechnology Applied to the Improvement of Cultures, Faculty of Sciences of Sfax, 3038 Sfax, Tunisia. Electronic address: rihab_b86@hotmail.com.
authors.forename: Rihab Ben Abdallah
authors.initials: RBA
authors.lastname: Kolsi
authors.affiliation: Chemistry Laboratory of Natural Substances, Faculty of Sciences of Sfax, PB 802, 3018 Sfax, Tunisia.
authors.forename: Jawhar
authors.initials: J
authors.lastname: Fakhfakh
authors.affiliation: Laboratory Enzyme and Bioconversion, National School of Engineering of Sfax, University of Sfax, 3038 Sfax, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Krichen
authors.affiliation: Biodiversity Unit and aquatic ecosystems, Faculty of Sciences of Sfax, 3038 Sfax, Tunisia.
authors.forename: Imed
authors.initials: I
authors.lastname: Jribi
authors.affiliation: Stazione Zoologica 'A. Dohrn', Functional and Evolutionary Ecology Laboratory, Punta S. Pietro, 80077 Ischia, Naples, Italy.
authors.forename: Antonia
authors.initials: A
authors.lastname: Chiarore
authors.affiliation: Stazione Zoologica 'A. Dohrn', Functional and Evolutionary Ecology Laboratory, Punta S. Pietro, 80077 Ischia, Naples, Italy.
authors.forename: Francesco Paolo
authors.initials: FP
authors.lastname: Patti
authors.affiliation: Unité de Technologie des Industries Agro-alimentaires, Faculté des Sciences Agronomiques de Gembloux, passage des Déportés 2, 5030 Gembloux, Belgium.
authors.forename: Christophe
authors.initials: C
authors.lastname: Blecker
authors.affiliation: Chemistry Laboratory of Natural Substances, Faculty of Sciences of Sfax, PB 802, 3018 Sfax, Tunisia.
authors.forename: Noureddine
authors.initials: N
authors.lastname: Allouche
authors.affiliation: Biotechnology Center of Sfax, Enzyme and Bioconversion Unit, University of Sfax, PB 1177, 3018 Sfax, Tunisia.
authors.forename: Hafedh
authors.initials: H
authors.lastname: Belghith
authors.affiliation: Laboratory of Plant Biotechnology Applied to the Improvement of Cultures, Faculty of Sciences of Sfax, 3038 Sfax, Tunisia.
authors.forename: Karima
authors.initials: K
authors.lastname: Belghith
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry*; D000959:Antihypertensive Agents / Q000737:chemistry*; D019684:Magnoliopsida; D015394:Molecular Structure; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D011134:Polysaccharides; D013431:Sulfates; D007703:Peptidyl-Dipeptidase A
keywords: Antihypertensive properties; LC–ESI–MS/MS analysis; Marine angiosperm; NMR spectroscopy; Sulfated polysaccharide