title: [Senescence-associated Beta Galactosidase Expression in Rat Schwann Cells after Chronic Denervation].
abstract: OBJECTIVE
  To investigate the effect of prolonged axon depletion on senescence-associated beta galactosidase (SA-β-gal) expression in Schwann cells (SCs) of adult rats.
  
  
  METHODS
  Male adult Sprague-Dawley (SD) rats were randomize grouped into sham-operated group and denervation groups for 1 week, 2 weeks, 4 weeks, 5 weeks, 6 weeks, 7 weeks and 8 weeks. Rats were subjected to right sciatic nerve transection. After particular denervation duration for the distal stumps, animals were anesthetized and perfused. Proximal stumps of 5 mm and distal stumps of 10 mm from injured nerves, and the corresponding segments from the sham groups and contralateral nerves were harvested and prepared for SA-β-gal staining to detect SA-β-gal expression. Then, additional injured distal stumps denervated for 8 weeks were employed for determining cellular distribution of SA-β-gal expression by co-labeling of SA-β-gal and SC-specific protein (S100β).
  
  
  RESULTS
  SA-β-gal expression transiently increased in distal tips of proximal stumps 2 weeks after adult rat sciatic nerve transection without suture. In contrast, in the distal stumps of transected adult rat sciatic nerves, axon depletion for 2 weeks increased SA-β-gal expression, and the increased expression of SA-β-gal remained constant after prolonged denervation durations. Furthermore, combination of SA-β-gal staining with S100β immunofluorescence staining showed that SA-β-gal expression. was exclusively present in denervated SCs.
  
  
  CONCLUSIONS
  Prolonged axon depletion increased SA-β-gal expression in adult rat SCs.
authors.forename: Xia
authors.initials: X
authors.lastname: Xiao
authors.forename: Ze-ka
authors.initials: ZK
authors.lastname: Chen
authors.forename: You-quan
authors.initials: YQ
authors.lastname: Ding
authors.forename: Xuan-yang
authors.initials: XY
authors.lastname: Li
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Qi
mesh_terms: D000818:Animals; D003714:Denervation; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit / Q000378:metabolism; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000601:surgery; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D064568:S100 Calcium Binding Protein beta Subunit; C579158:S100b protein, rat; D001616:beta-Galactosidase