title: Radiation Synthesis of Poly(Starch/Acrylic acid) pH Sensitive Hydrogel for Rutin Controlled Release.
abstract: The copolymerization of starch with acrylic acid AAc using direct gamma radiation technique was performed. The effect of AAc concentrations on the gel (%) and swelling behavior were investigated. It is found that as AAc concentrations increase both gel(%) and swelling behavior increase. The Poly(starch/acrylic acid) (1:10wt%) hydrogel were selected due to its high swelling properties. From the in-vitro release study of the rutin-loaded hydrogel it is observed that it is strong pH-dependent release behavior, thus offering a maximum release as pH increased. The dextran sulphate sodium (DSS)-induced rat colitis model was treated with rutin-loaded Poly(starch/acrylic acid) (1:10wt%) hydrogel and free rutin solution by oral administration. Colitic control group showed a significant elevation in colon/body weight ratio, myeloperoxgidase activity, tumor necrosis factor, nitric oxide and malondialdehyde levels. However, glutathione level was reduced. It was found that the rutin-loaded hydrogel was more efficient than free rutin as evidenced by improvement of all measured parameters. These effects were confirmed histopathologically and may be attributed to its ability to control delivery of rutin to colon with minor early release of rutin before colon. The Poly(starch/acrylic acid) (1:10wt%) can represent a pivotal anti-inflammatory approach for patients with inflammatory bowel disease in order to increase efficacy and reduce toxicity.
authors.affiliation: Radiation Research of Polymer chemistry Department, Industrial Irradiation Division. Electronic address: am_abdelghaffar@yahoo.com.
authors.forename: A M
authors.initials: AM
authors.lastname: Abdel Ghaffar
authors.affiliation: Drug Radiation Research Department, Biotechnology Division.
authors.forename: Rasha R
authors.initials: RR
authors.lastname: Radwan
authors.affiliation: Radiation Chemistry Department, Radiation Research Division, National Center for Radiation Research and Technology, Atomic Energy Authority, P.O. Box 29, Nasr City, Cairo, Egypt.
authors.forename: H E
authors.initials: HE
authors.lastname: Ali
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003106:Colon / Q000187:drug effects / Q000473:pathology; D003692:Delayed-Action Preparations; D005720:Gamma Rays; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis*; D006863:Hydrogen-Ion Concentration; D008297:Male; D009929:Organ Size / Q000187:drug effects; D017208:Rats, Wistar; D012431:Rutin / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013213:Starch / Q000138:chemical synthesis*; D013997:Time Factors
chemical_list: D000180:Acrylic Resins; D003692:Delayed-Action Preparations; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C006903:carbopol 940; D012431:Rutin; D013213:Starch
keywords: In-vitro study and in vivo evaluation; Radiation; Rutin controlled release