title: Investigation of septin biology in vivo using zebrafish.
abstract: The zebrafish (Danio rerio) is an important animal model to study cell biology in vivo. Benefits of the zebrafish include a fully annotated reference genome, an easily manipulable genome (for example, by morpholino oligonucleotide or CRISPR-Cas9), and transparent embryos for noninvasive, real-time microscopy using fluorescent transgenic lines. Zebrafish have orthologues of most human septins, and studies using larvae were used to investigate the role of septins in vertebrate development. The zebrafish larva is also an established model to study the cell biology of infection and has recently been used to visualize septin assembly during bacterial infection in vivo. Here, we describe protocols for the study of septins in zebrafish, with emphasis on techniques used to investigate the role of septins in host defense against bacterial infection.
authors.affiliation: Imperial College London, London, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: Willis
authors.affiliation: Imperial College London, London, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Mazon-Moya
authors.affiliation: Imperial College London, London, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Mostowy
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001419:Bacteria / Q000235:genetics / Q000472:pathogenicity; D001424:Bacterial Infections / Q000235:genetics* / Q000382:microbiology; D004195:Disease Models, Animal; D004625:Embryo, Nonmammalian / Q000382:microbiology; D047108:Embryonic Development / Q000235:genetics*; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D060172:Morpholinos / Q000235:genetics; D058112:Septins / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology
chemical_list: D060172:Morpholinos; D058112:Septins
keywords: Cytoskeleton; Host defense; Septin; Shigella; Zebrafish