title: Transcriptome Analysis and Postprandial Expression of Amino Acid Transporter Genes in the Fast Muscles and Gut of Chinese Perch (Siniperca chuatsi).
abstract: The characterization of the expression and regulation of growth-related genes in the muscles of Chinese perch is of great interest to aquaculturists because of the commercial value of the species. The transcriptome annotation of the skeletal muscles is a crucial step in muscle growth-related gene analysis. In this study, we generated 52 504 230 reads of mRNA sequence data from the fast muscles of the Chinese perch by using Solexa/Illumina RNA-seq. Twenty-one amino acid transporter genes were annotated by searching protein and gene ontology databases, and postprandial changes in their transcript abundance were assayed after administering a single satiating meal to Chinese perch juveniles (body mass, approximately 100 g), following fasting for 1 week. The gut content of the Chinese perch increased significantly after 1 h and remained high for 6 h following the meal and emptied within 48-96 h. Expression of eight amino acid transporter genes was assayed in the fast muscles through quantitative real-time polymerase chain reaction at 0, 1, 3, 6, 12, 24, 48, and 96 h. Among the genes, five transporter transcripts were markedly up-regulated within 1 h of refeeding, indicating that they may be potential candidate genes involved in the rapid-response signaling system regulating fish myotomal muscle growth. These genes display coordinated regulation favoring the resumption of myogenesis responding to feeding.
authors.affiliation: Department of Bioengneering and Environmental Science, Changsha University, Changsha, 410003, China.|Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, 415000, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.affiliation: Department of Bioengneering and Environmental Science, Changsha University, Changsha, 410003, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Bioengneering and Environmental Science, Changsha University, Changsha, 410003, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Bioengneering and Environmental Science, Changsha University, Changsha, 410003, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Hunan Aquaculture and Fishes, Changsha, 410005, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zeng
authors.affiliation: Institute of Hunan Aquaculture and Fishes, Changsha, 410005, China.
authors.forename: Yuanan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Bioengneering and Environmental Science, Changsha University, Changsha, 410003, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Bioengneering and Environmental Science, Changsha University, Changsha, 410003, China.|Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, 415000, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Bioengneering and Environmental Science, Changsha University, Changsha, 410003, China.|Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, 415000, China.
authors.forename: Wuying
authors.identifier: http://orcid.org/0000-0003-1248-122X
authors.initials: W
authors.lastname: Chu
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism*; D010471:Perches / Q000235:genetics* / Q000378:metabolism; D019518:Postprandial Period; D059467:Transcriptome
chemical_list: D026905:Amino Acid Transport Systems; D029941:Fish Proteins