title: Effect of Endometrial Stem Cell-derived Cytokine Cocktail on a Mouse Model of Myocardial Reperfusion Injury.
abstract: Objective To study the effect of endometrial stem cells (EnSCs) derived cytokine cocktail (EdCC) on myocardial ischemic reperfusion injury (I/R) in a mouse model. Methods EdCC was concentrated from the culture medium of EnSCs with Millipore ultra-filtration technology and was administrated to a myocardial I/R mouse models through tail vein injection. The infarct area was determined by TTC/Evans Blue staining. The apoptotic cells were counted by TUNEL assay and the protein level of cleaved caspase 3 was evaluated by Western blotting.  Results  The EdCC extraction efficiency was (222.4±29.3) Μg/10(6) cells in every 24 h,but the protein gradually degraded under-80 ℃ storage. As compared with I/R group,100 Μg fresh EdCC decreased infarct area (P=0.001),reduced apoptotic nuclei in the infarct border (P=0.019),and inhibited cleaved caspase 3 expression (P=0.002). Increasing EdCC dosage did not further reduce the infarct area. The myocardial protective effect of EdCC diminished after 90 days' storage under-80 ℃.  Conclusion EdCC reduces myocardial I/R injury through protecting cardiomyocytes from apoptosis within 30 days storage under-80 ℃.
authors.affiliation: Department of Cardiology,Guizhou Provincial People’s Hospital,Guizhou 550002,China
authors.forename: Zhi
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Clinical Medicine,Guizhou Medical University,Guizhou 550025,China
authors.forename: Zhong-shen
authors.initials: ZS
authors.lastname: Jia
authors.affiliation: Department of Cardiology,Guizhou Provincial People’s Hospital,Guizhou 550002,China|Department of Clinical Medicine,Guizhou Medical University,Guizhou 550025,China
authors.forename: Yu-jia
authors.initials: YJ
authors.lastname: Pan
authors.affiliation: Department of Cardiology,Guizhou Provincial People’s Hospital,Guizhou 550002,China
authors.forename: An-jie
authors.initials: AJ
authors.lastname: Li
authors.affiliation: Department of Cardiology,Guizhou Provincial People’s Hospital,Guizhou 550002,China
authors.forename: Fang
authors.initials: F
authors.lastname: Wei
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004717:Endometrium / Q000166:cytology*; D005260:Female; D051379:Mice; D015428:Myocardial Reperfusion Injury / Q000628:therapy*; D032383:Myocytes, Cardiac / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology*
chemical_list: D016207:Cytokines; C505409:Casp3 protein, mouse; D053148:Caspase 3