title: Lobaplatin for the treatment of SK-MES-1 lung squamous cell line in vitro and in vivo.
abstract: Lung squamous cell carcinoma is the second-largest histological subtype of lung cancer, which is the leading cause of cancer-related death worldwide. Lobaplatin, one of the third-generation platinum compounds, has shown encouraging anticancer activity in a variety of tumors. The aim of this study was to determine the therapeutic efficacy of lobaplatin on p53-mutant lung squamous cancer cells SK-MES-1. In order to evaluate the antitumor effect of lobaplatin, several in vitro and in vivo analyses were carried out, including Cell Counting Kit-8 (CCK-8), fluorescence-activated cell sorter, Western blot, and terminal deoxynucleotidyl transferase dUTP nick end labeling. Findings showed that lobaplatin could inhibit cell proliferation and induce apoptosis of SK-MES-1 cells in vitro through both intrinsic and extrinsic apoptotic pathways in a time- and dose-dependent manner. In addition, lobaplatin could arrest cell cycle at S phase in SK-MES-1. Lobaplatin has obvious antitumor efficacy in human SK-MES-1 xenograft models; therefore, it seems to be a promising candidate in lung squamous cancer therapy.
authors.affiliation: Department of Pulmonary Medicine.
authors.forename: Hongming
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu Province.
authors.forename: Runzhe
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Pulmonary Oncology, The 307th Hospital of Chinese People's Liberation Army, Beijing.
authors.forename: Shaoxing
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Pulmonary Oncology, The 307th Hospital of Chinese People's Liberation Army, Beijing.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Medical Oncology, Jiangsu Cancer Hospital, Nanjing, Jiangsu Province, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu Province.
authors.forename: Haijun
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Pulmonary Medicine.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu Province.
authors.forename: Baoan
authors.initials: B
authors.lastname: Chen
keywords: apoptosis; cell cycle arrest; lobaplatin; lung squamous cell carcinoma