title: Galangin Induces Autophagy via Deacetylation of LC3 by SIRT1 in HepG2 Cells.
abstract: Galangin suppresses proliferation and induces apoptosis and autophagy in hepatocellular carcinoma (HCC) cells, but the precise mechanism is not clear. In this study, we demonstrated that galangin induced autophagy, enhanced the binding of SIRT1-LC3 and reduced the acetylation of endogenous LC3 in HepG2 cells. But this autophagy was inhibited by inactivation of SIRT1 meanwhile, galangin failed to reduce the acetylation of endogenous LC3 after SIRT1 was knocked-down. Collectively, these findings demonstrate a new mechanism by which galangin induces autophagy via the deacetylation of endogenous LC3 by SIRT1.
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
authors.forename: Xv
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.|Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.|Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Ding
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
authors.forename: Liubo
authors.initials: L
authors.lastname: Lan
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000107:Acetylation / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000648:ultrastructure; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000648:ultrastructure; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008954:Models, Biological; D056564:Sirtuin 1 / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D005419:Flavonoids; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; C037032:galangin; D056564:Sirtuin 1