title: The effect of melatonin and vitamin C treatment on the experimentally induced tympanosclerosis: study in rats.
abstract: BACKGROUND
  The ethiopathogenesis of tympanosclerosis has not been completely under- stood yet. Recent studies have shown that free oxygen radicals are important in the formation of tympanosclerosis. Melatonin and Vitamin C are known to be a powerful antioxidant, interacts directly with Reactive Oxygen Species and controls free radical-mediated tissue damage.
  
  
  OBJECTIVE
  To demonstrate the possible preventative effects of melatonin and Vitamin C on tympanosclerosis in rats by using histopathology and determination of total antioxidant status total antioxidant status.
  
  
  METHODS
  Standard myringotomy and standard injury were performed in the middle ear of 24 rats. The animals were divided into three groups: Group 1 received melatonin, Group 2 received vitamin C, and Group 3 received saline solution.
  
  
  RESULTS
  The mean values of total antioxidant status were similar in the all study groups before the treatment period. The mean values of total antioxidant status were significantly higher in the melatonin and vitamin C groups compared to control group but vitamin C with melatonin groups were similar after the treatment period (p<0.001). Minimum and maximum wall thicknesses were lower in the melatonin and vitamin C groups compared to the control group but the differences were insignificant.
  
  
  CONCLUSIONS
  Melatonin increases total antioxidant status level and might have some effect on tympanosclerosis that develops after myringotomy.
authors.affiliation: Antalya Education and Research Hospital, Department of ENT Head and Neck Surgery, Antalya, Turkey. Electronic address: drsemakoc@gmail.com.
authors.forename: Sema
authors.initials: S
authors.lastname: Koc
authors.affiliation: Mevlana University, School of Medicine, Department of Pathology, Konya, Turkey.
authors.forename: Halil
authors.initials: H
authors.lastname: Kıyıcı
authors.affiliation: Necmettin Erbakan University, School of Medicine, Department of Biochemistry and Clinical Biochemistry, Konya, Turkey.
authors.forename: Aysun
authors.initials: A
authors.lastname: Toker
authors.affiliation: Gaziosmanpasa University, School of Medicine, Department of Otorhinolaryngology, Tokat, Turkey.
authors.forename: Harun
authors.initials: H
authors.lastname: Soyalıç
authors.affiliation: Gaziosmanpasa University, School of Medicine, Department of Histology and Embryology, Tokat, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Aslan
authors.affiliation: Gaziosmanpasa University, School of Medicine, Department of Histology and Embryology, Tokat, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Kesici
authors.affiliation: Gaziosmanpasa University, School of Medicine, Department of Histology and Embryology, Tokat, Turkey.
authors.forename: Zafer I
authors.initials: ZI
authors.lastname: Karaca
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001205:Ascorbic Acid / Q000008:administration & dosage*; D004195:Disease Models, Animal; D008297:Male; D008550:Melatonin / Q000008:administration & dosage*; D063371:Myringosclerosis / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D014432:Tympanic Membrane / Q000187:drug effects; D014815:Vitamins / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D014815:Vitamins; D008550:Melatonin; D001205:Ascorbic Acid
keywords: Capacidade antioxidante total; Melatonin; Melatonina; Timpanoesclerose; Total antioxidant status; Tympanosclerosis; Vitamin C; Vitamina C