title: A Vesicle Supra-Assembly Approach to Synthesize Amine-Functionalized Hollow Dendritic Mesoporous Silica Nanospheres for Protein Delivery.
abstract: Intracellular delivery of proteins is a promising strategy of intervention in disease, which relies heavily on the development of efficient delivery platforms due to the cell membrane impermeability of native proteins, particularly for negatively charged large proteins. This work reports a vesicle supra-assembly approach to synthesize novel amine-functionalized hollow dendritic mesoporous silica nanospheres (A-HDMSN). An amine silica source is introduced into a water-oil reaction solution prior to the addition of conventional silica source tetraethylorthosilicate. This strategy favors the formation of composite vesicles as the building blocks which further assemble into the final product. The obtained A-HDMSN have a cavity core of ≈170 nm, large dendritic mesopores of 20.7 nm in the shell and high pore volume of 2.67 cm3g-1. Compared to the calcined counterpart without amine groups (C-HDMSN), A-HDMSN possess enhanced loading capacity to large negative proteins (IgG and β-galactosidase) and improved cellular uptake performance, contributed by the cationic groups. A-HDMSN enhance the intracellular uptake of β-galactosidase by up to 5-fold and 40-fold compared to C-HDMSN and free β-galactosidase, respectively. The active form of β-galactosidase delivered by A-HDMSN retains its intracellular catalytic functions.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Anand Kumar
authors.initials: AK
authors.lastname: Meka
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Prasanna Lakshmi
authors.initials: PL
authors.lastname: Abbaraju
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Hao
authors.initials: H
authors.lastname: Song
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Chun
authors.initials: C
authors.lastname: Xu
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia. m.yu2@uq.edu.au.
authors.forename: Meihua
authors.initials: M
authors.lastname: Yu
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia. c.yu@uq.edu.au.
authors.forename: Chengzhong
authors.initials: C
authors.lastname: Yu
mesh_terms: D000588:Amines / Q000737:chemistry*; D000818:Animals; D016466:CHO Cells; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry; D007074:Immunoglobulin G / Q000008:administration & dosage; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D011506:Proteins / Q000008:administration & dosage*; D012822:Silicon Dioxide / Q000737:chemistry*; D013997:Time Factors; D001616:beta-Galactosidase / Q000008:administration & dosage
chemical_list: D000588:Amines; D007074:Immunoglobulin G; D011506:Proteins; D012822:Silicon Dioxide; D001616:beta-Galactosidase
keywords: amine functionalization; cellular uptake; hollow nanospheres; protein delivery; silica nanoparticles