title: A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells.
abstract: BACKGROUND
  Mesenchymal stromal cells (MSCs) are multipotent progenitor cells used in several cell therapies. MSCs are characterized by the expression of CD73, CD90, and CD105 cell markers, and the absence of CD34, CD45, CD11a, CD19, and HLA-DR cell markers. CD90 is a glycoprotein present in the MSC membranes and also in adult cells and cancer stem cells. The role of CD90 in MSCs remains unknown. Here, we sought to analyse the role that CD90 plays in the characteristic properties of in vitro expanded human MSCs.
  
  
  METHODS
  We investigated the function of CD90 with regard to morphology, proliferation rate, suppression of T-cell proliferation, and osteogenic/adipogenic differentiation of MSCs by reducing the expression of this marker using CD90-target small hairpin RNA lentiviral vectors.
  
  
  RESULTS
  The present study shows that a reduction in CD90 expression enhances the osteogenic and adipogenic differentiation of MSCs in vitro and, unexpectedly, causes a decrease in CD44 and CD166 expression.
  
  
  CONCLUSIONS
  Our study suggests that CD90 controls the differentiation of MSCs by acting as an obstacle in the pathway of differentiation commitment. This may be overcome in the presence of the correct differentiation stimuli, supporting the idea that CD90 level manipulation may lead to more efficient differentiation rates in vitro.
authors.affiliation: Departamento de Genética e Morfologia, Universidade de Brasília, Brasília, DF, Brazil.|Centro Universitario do Distrito Federal UDF, Brasília, DF, Brazil.
authors.forename: Daniela A
authors.initials: DA
authors.lastname: Moraes
authors.affiliation: Departamento de Neurologia e Neurocirurgia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Tatiana T
authors.initials: TT
authors.lastname: Sibov
authors.affiliation: Departamento de Neurologia e Neurocirurgia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Lorena F
authors.initials: LF
authors.lastname: Pavon
authors.affiliation: Departamento de Genética e Morfologia, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Paula Q
authors.initials: PQ
authors.lastname: Alvim
authors.affiliation: Departamento de Genética e Morfologia, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Raphael S
authors.initials: RS
authors.lastname: Bonadio
authors.affiliation: Departamento de Genética e Morfologia, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Jaqueline R
authors.initials: JR
authors.lastname: Da Silva
authors.affiliation: Departamento de Genética e Morfologia, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Pic-Taylor
authors.affiliation: Departamento de Ciências da Saúde, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Orlando A
authors.initials: OA
authors.lastname: Toledo
authors.affiliation: Hospital Israelita Albert Einstein, Instituto de Ensino e Pesquisa - Centro de Pesquisa Experimental São Paulo, São Paulo, SP, Brazil.
authors.forename: Luciana C
authors.initials: LC
authors.lastname: Marti
authors.affiliation: Departamento de Genética e Morfologia, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Ricardo B
authors.initials: RB
authors.lastname: Azevedo
authors.affiliation: Departamento de Genética e Morfologia, Universidade de Brasília, Brasília, DF, Brazil. dmoliveira@unb.br.|IB-Departamento de Genética e Morfologia, Universidade de Brasília - UNB, Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, CEP 70910-970, Brazil. dmoliveira@unb.br.
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Oliveira
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000653:Amniotic Fluid / Q000166:cytology / Q000378:metabolism; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism; D005326:Fetal Proteins / Q000235:genetics / Q000378:metabolism; D020868:Gene Silencing; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism; D016130:Immunophenotyping; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D061251:Primary Cell Culture; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D018800:Thy-1 Antigens / Q000235:genetics* / Q000378:metabolism; D020410:Activated-Leukocyte Cell Adhesion Molecule
chemical_list: D015703:Antigens, CD; D015816:Cell Adhesion Molecules, Neuronal; D005326:Fetal Proteins; D018960:Hyaluronan Receptors; D034741:RNA, Small Interfering; D018800:Thy-1 Antigens; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule; C497483:CD44 protein, human
keywords: CD90; Differentiation; Fibroblast; Mesenchymal stem cells; Mesenchymal stromal cells; Thy-1