title: S18 family of mitochondrial ribosomal proteins: evolutionary history and Gly132 polymorphism in colon carcinoma.
abstract: S18 family of mitochondrial ribosomal proteins (MRPS18, S18) consists of three members, S18-1 to -3. Earlier, we found that overexpression of S18-2 protein resulted in immortalization and eventual transformation of primary rat fibroblasts. The S18-1 and -3 have not exhibited such abilities. To understand the differences in protein properties, the evolutionary history of S18 family was analyzed. The S18-3, followed by S18-1 and S18-2 emerged as a result of ancient gene duplication in the root of eukaryotic species tree, followed by two metazoan-specific gene duplications. However, the most conserved metazoan S18 homolog is the S18-1; it shares the most sequence similarity with S18 proteins of bacteria and of other eukaryotic clades. Evolutionarily conserved residues of S18 proteins were analyzed in various cancers. S18-2 is mutated at a higher rate, compared with S18-1 and -3 proteins. Moreover, the evolutionarily conserved residue, Gly132 of S18-2, shows genetic polymorphism in colon adenocarcinomas that was confirmed by direct DNA sequencing.Concluding, S18 family represents the yet unexplored important mitochondrial ribosomal proteins.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institute, Stockholm, S-17177, Sweden.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Mushtaq
authors.affiliation: KTH Royal Institute of Technology, Science for Life Laboratory, School of Computer Science and Communication, Solna, SE-17 177, Sweden.
authors.forename: Raja Hashim
authors.initials: RH
authors.lastname: Ali
authors.affiliation: Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institute, Stockholm, S-17177, Sweden.|Institute of Molecular Biology and Genetics of NASU, Kyiv, 03680, Ukraine.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Kashuba
authors.affiliation: Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institute, Stockholm, S-17177, Sweden.
authors.forename: George
authors.initials: G
authors.lastname: Klein
authors.affiliation: Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institute, Stockholm, S-17177, Sweden.|R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU, Kyiv, 03022, Ukraine.
authors.forename: Elena
authors.initials: E
authors.lastname: Kashuba
mesh_terms: D003110:Colonic Neoplasms / Q000235:genetics*; D019143:Evolution, Molecular; D006801:Humans; D024101:Mitochondrial Proteins / Q000235:genetics*; D009154:Mutation; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012269:Ribosomal Proteins / Q000235:genetics*
chemical_list: C000621827:MRPS18C protein, human; D024101:Mitochondrial Proteins; D012269:Ribosomal Proteins; C041184:ribosomal protein S18
keywords: MRPS18 family; evolutionary trace analysis; genetic polymorphism; mitochondrial ribosomal proteins; phylogenetic analysis