title: Oral exposure to commercially available coal tar-based pavement sealcoat induces murine genetic damage and mutations.
abstract: Coal tar (CT) is a thick black liquid produced as a by-product of coal carbonization to produce coke or manufactured gas. It is comprised a complex mixture of polycyclic aromatic compounds, including a wide range of polycyclic aromatic hydrocarbons (PAHs), many of which are genotoxic and carcinogenic. CT is used in some pavement sealants (also known as sealcoat), which are applied to pavement in order to seal and beautify the surface. Human exposure is known to occur not only during application, but also as a result of the weathering process, as elevated levels of PAHs have been found in settled house dust in residences adjacent to CT-sealed surfaces. In this study we examined the genotoxicity of an extract of a commercially available CT-based sealcoat in the transgenic Muta™Mouse model. Mice were orally exposed to 3 doses of sealcoat extract daily for 28 days. We evaluated genotoxicity by examining: (1) stable DNA adducts and (2) lacZ mutations in bone marrow, liver, lung, small intestine, and glandular stomach, as well as (3) micronucleated red blood cells. Significant increases were seen for each endpoint and in all tissues. The potency of the response differed across tissues, with the highest frequency of adducts occurring in liver and lung, and the highest frequency of mutations occurring in small intestine. The results of this study are the first demonstration of mammalian genotoxicity following exposure to CT-containing pavement sealcoat. This work provides in vivo evidence to support the contention that there may be adverse health effects in mammals, and potentially in humans, from exposure to coal tar. Environ. Mol. Mutagen. 57:535-545, 2016. © 2016 Her Majesty the Queen in Right of Canada.
authors.affiliation: Department of Biology, University of Ottawa, Ottawa, Ontario, Canada.|Mechanistic Studies Division, Environmental Health Science and Research Bureau, Environmental and Radiation Health Sciences Directorate, HECSB, Health Canada, Ottawa, Ontario, Canada.
authors.forename: Alexandra S
authors.initials: AS
authors.lastname: Long
authors.affiliation: Mechanistic Studies Division, Environmental Health Science and Research Bureau, Environmental and Radiation Health Sciences Directorate, HECSB, Health Canada, Ottawa, Ontario, Canada.
authors.forename: Margaret
authors.initials: M
authors.lastname: Watson
authors.affiliation: Analytical and Environmental Sciences Division, MRC-PHE Centre for Environment and Health, King's College London, London, United Kingdom.
authors.forename: Volker M
authors.initials: VM
authors.lastname: Arlt
authors.affiliation: Department of Biology, University of Ottawa, Ottawa, Ontario, Canada.|Mechanistic Studies Division, Environmental Health Science and Research Bureau, Environmental and Radiation Health Sciences Directorate, HECSB, Health Canada, Ottawa, Ontario, Canada.
authors.forename: Paul A
authors.initials: PA
authors.lastname: White
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003033:Coal Tar / Q000737:chemistry / Q000633:toxicity*; D003252:Construction Materials / Q000633:toxicity*; D018736:DNA Adducts / Q000032:analysis*; D005434:Flow Cytometry; D007763:Lac Operon / Q000235:genetics*; D008822:Mice, Transgenic; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D009152:Mutagenicity Tests / Q000379:methods; D009154:Mutation; D009928:Organ Specificity; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis
chemical_list: D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; D003033:Coal Tar
keywords: BaP; PAH; coal tar; driveway sealant; genotoxicity; sealcoat