title: Studying Lipid Metabolism and Transport During Zebrafish Development.
abstract: The zebrafish model facilitates the study of lipid metabolism and transport during development. Here, we outline methods to introduce traceable fluorescent or radiolabeled fatty acids into zebrafish embryos and larvae at various developmental stages. Labeled fatty acids can be injected into the large yolk cell prior to the development of digestive organs when the larvae is entirely dependent on the yolk for its nutrition (lecithotrophic state). Once zebrafish are able to consume exogenous food, labeled fatty acids can be incorporated into their food. Our group and others have demonstrated that the transport and processing of these injected or ingested fatty acid analogs can be followed through microscopy and/or biochemical analysis. These techniques can be easily combined with targeted antisense approaches, transgenics, or drug treatments (see Note 1 ), allowing studies of lipid cell biology and metabolism that are exceedingly difficult or impossible in mammals.
authors.affiliation: Department of Embryology, Carnegie Institution for Science, 3520 San Martin Drive, Baltimore, MD, USA.
authors.forename: Erin M
authors.initials: EM
authors.lastname: Zeituni
authors.affiliation: Department of Embryology, Carnegie Institution for Science, 3520 San Martin Drive, Baltimore, MD, USA. farber@ciwemb.edu.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Farber
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D007814:Larva / Q000235:genetics / Q000378:metabolism*; D050356:Lipid Metabolism / Q000235:genetics / Q000502:physiology*; D015027:Zebrafish / Q000196:embryology / Q000378:metabolism*
keywords: Lipid; Metabolism; Microscopy; Thin layer chromatography; Transport; Zebrafish