title: Isolation and identification of cellulose-producing strain Komagataeibacter intermedius from fermented fruit juice.
abstract: A bacterial cellulose (BC) producing strain isolated from fermented fruit juice was identified as Komagataeibacter intermedius (K. intermedius) FST213-1 by 16s rDNA sequencing analysis and biochemical characteristics test. K. intermedius FST213-1 can produce BC within pH 4-9 and exhibit maximum BC production (1.2g/L) at pH 8 in short-term (4-day) cultivation. Results of Fourier transform infrared spectroscopy, X-ray diffraction, water content, thermogravimetric analysis and mechanical property indicated that BC produced from K. intermedius FST213-1 exhibits higher water content ability (99.5%), lower thermostability (315°C), lower crystallinity (79.3%) and similar mechanical properties in comparison with the specimen from model BC producer, Gluconacetobacter xylinus 23769. Based on these analyses, the novel based-resistant strain K. intermedius FST213-1 can efficiently produce BC, which can be applied for industrial manufacturing with potential features.
authors.affiliation: Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan. Electronic address: d00642009@ntu.edu.tw.
authors.forename: Shin-Ping
authors.initials: SP
authors.lastname: Lin
authors.affiliation: Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan. Electronic address: b02603033@ntu.edu.tw.
authors.forename: Yin-Hsuan
authors.initials: YH
authors.lastname: Huang
authors.affiliation: Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan. Electronic address: twhsu72@gmail.com.
authors.forename: Kai-Di
authors.initials: KD
authors.lastname: Hsu
authors.affiliation: Department of Food Science, National Quemoy University, Kinmen 89250, Taiwan. Electronic address: d91641006@gmail.com.
authors.forename: Ying-Jang
authors.initials: YJ
authors.lastname: Lai
authors.affiliation: Department of Food Science, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. Electronic address: chenyk@mail.npust.edu.tw.
authors.forename: Yu-Kuo
authors.initials: YK
authors.lastname: Chen
authors.affiliation: Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan; Graduate Institute of Food Science Technology, National Taiwan University, Taipei 10617, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan. Electronic address: kccheng@ntu.edu.tw.
authors.forename: Kuan-Chen
authors.initials: KC
authors.lastname: Cheng
mesh_terms: D016947:Acetobacteraceae / Q000302:isolation & purification* / Q000378:metabolism*; D002482:Cellulose / Q000096:biosynthesis*; D005285:Fermentation; D000067030:Fruit and Vegetable Juices / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D013696:Temperature
chemical_list: D002482:Cellulose
keywords: Bacterial cellulose; Base-resistant strain; Komagataeibacter intermedius; Materials property analysis; Strain selection