title: Preparation of Hydrochlorothiazide Nanoparticles for Solubility Enhancement.
abstract: Nanoparticles can be considered as a useful tool for improving properties of poorly soluble active ingredients. Hydrochlorothiazide (Class IV of the Biopharmaceutical Classification System) was chosen as a model compound. Antisolvent precipitation-solvent evaporation and emulsion solvent evaporation methods were used for preparation of 18 samples containing hydrochlorothiazide nanoparticles. Water solutions of surfactants sodium dodecyl sulfate, Tween 80 and carboxymethyl dextran were used in mass concentrations of 1%, 3% and 5%. Acetone and dichloromethane were used as solvents of the model compound. The particle size of the prepared samples was measured by dynamic light scattering. The selected sample of hydrochlorothiazide nanoparticles stabilized with carboxymethyl dextran sodium salt with particle size 2.6 nm was characterized additionally by Fourier transform mid-infrared spectroscopy and scanning electron microscopy. It was found that the solubility of this sample was 6.5-fold higher than that of bulk hydrochlorothiazide.
authors.affiliation: Nanotechnology Centre, VSB-Technical University of Ostrava, 17. listopadu 15/2172, 708 33 Ostrava, Czech Republic. eliskavaculikova@seznam.cz.
authors.forename: Eliska
authors.initials: E
authors.lastname: Vaculikova
authors.affiliation: Department of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1/3, 612 42 Brno, Czech Republic. cernikova.aneta@gmail.com.
authors.forename: Aneta
authors.initials: A
authors.lastname: Cernikova
authors.affiliation: Nanotechnology Centre, VSB-Technical University of Ostrava, 17. listopadu 15/2172, 708 33 Ostrava, Czech Republic. daniela.placha@vsb.cz.|IT4 Innovations Centrum Excellence, VSB-Technical University of Ostrava, 17. listopadu 15/2172, 708 33 Ostrava, Czech Republic. daniela.placha@vsb.cz.
authors.forename: Daniela
authors.initials: D
authors.lastname: Placha
authors.affiliation: Department of Chemical Theory of Drugs, Faculty of Pharmacy, Comenius University, Kalinciakova 8, 832 32 Bratislava, Slovakia. pisarcik@fpharm.uniba.sk.
authors.forename: Martin
authors.initials: M
authors.lastname: Pisarcik
authors.affiliation: Nanotechnology Centre, VSB-Technical University of Ostrava, 17. listopadu 15/2172, 708 33 Ostrava, Czech Republic. pavlina.peikertova@vsb.cz.|IT4 Innovations Centrum Excellence, VSB-Technical University of Ostrava, 17. listopadu 15/2172, 708 33 Ostrava, Czech Republic. pavlina.peikertova@vsb.cz.
authors.forename: Pavlina
authors.initials: P
authors.lastname: Peikertova
authors.affiliation: Nanotechnology Centre, VSB-Technical University of Ostrava, 17. listopadu 15/2172, 708 33 Ostrava, Czech Republic. katerina.dedkova@vsb.cz.|Regional Materials Science and Technology Centre, VSB-Technical University of Ostrava, 17. listopadu 15/2172, 708 33 Ostrava, Czech Republic. katerina.dedkova@vsb.cz.
authors.forename: Katerina
authors.initials: K
authors.lastname: Dedkova
authors.affiliation: Department of Chemical Theory of Drugs, Faculty of Pharmacy, Comenius University, Kalinciakova 8, 832 32 Bratislava, Slovakia. devinsky@fpharm.uniba.sk.
authors.forename: Ferdinand
authors.initials: F
authors.lastname: Devinsky
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University, Odbojarov 10, 832 32 Bratislava, Slovakia. josef.jampilek@gmail.com.
authors.forename: Josef
authors.initials: J
authors.lastname: Jampilek
mesh_terms: D004339:Drug Compounding / Q000379:methods; D000067493:Dynamic Light Scattering; D006852:Hydrochlorothiazide / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D012997:Solvents; D013501:Surface-Active Agents; D006852:Hydrochlorothiazide
keywords: dynamic light scattering; hydrochlorothiazide; infrared spectroscopy; nanoparticles; scanning electron microscopy; solubility