title: Validation of a Fluorescence Sensor Microtiterplate for Biogenic Amines in Meat and Cheese.
abstract: An optical sensor microtiterplate for quantitative analysis of the total content of biogenic amines (TAC) in meat and cheese was developed and validated for the first time. In the plate, a chameleon dye (Py-1) is embedded in a polymeric cocktail which is deposited on the bottom of the wells in a common microtiterplate. On reaction with biogenic amines (BAs), the fluorescence of Py-1 at 620 nm rapidly delivers a precise TAC. After 10 min incubation at 25 °C the determination of the TAC in various (real) samples is possible in high-throughput with a standard microplate reader. The optimized fluorescence method was validated for linearity, sensitivity, accuracy, precision (intraday and inter day repeatability) and recovery using histamine (HIS) as a representative BA. The sensor microtiterplate was successfully applied to quantitatively analyze the TAC in 10 real samples of cheese and meat obtained from various Egyptian markets. The TAC of these real samples obtained by the sensor microtiterplate was validated against the contents of BAs obtained by GC-MS at various times of storage. The data of the sensor microtiterplate agreed well with those of GC-MS. This demonstrates that the sensor microtiterplate is a reliable screening tool for the degradation status of food samples.
authors.affiliation: Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt.|Chemistry Department, Faculty of Science and Arts, Aljouf University, P.O. Box # 2014, Skaka-41421, Kingdom of Saudi Arabia.
authors.forename: Gasser M
authors.initials: GM
authors.lastname: Khairy
authors.affiliation: Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt.
authors.forename: Hassan A
authors.initials: HA
authors.lastname: Azab
authors.affiliation: Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt.
authors.forename: Sabry A
authors.initials: SA
authors.lastname: El-Korashy
authors.affiliation: Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93040, Regensburg, Germany.
authors.forename: Mark-Steven
authors.initials: MS
authors.lastname: Steiner
authors.affiliation: Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93040, Regensburg, Germany. axel.duerkop@ur.de.
authors.forename: Axel
authors.identifier: http://orcid.org/0000-0003-3007-6471
authors.initials: A
authors.lastname: Duerkop
mesh_terms: D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D002611:Cheese / Q000032:analysis*; D005453:Fluorescence; D005504:Food Analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D057166:High-Throughput Screening Assays / Q000379:methods*; D008460:Meat / Q000032:analysis*
chemical_list: D001679:Biogenic Amines
keywords: Biogenic amines; Cheese; Fluorescence; GC-MS; Meat; Microtiterplate; Sensor