title: Cimetidine disrupts the renewal of testicular cells and the steroidogenesis in a hermaphrodite fish.
abstract: The importance of histamine in the physiology of the testis in mammals and reptiles has been recently shown. Histamine receptors (Hrs) are well conserved in fish and are functional in several fish species. We report here for the first time that histamine and the mRNA of Hrh1, Hrh2 and Hrh3 are all present in the gonad of the hermaphrodite teleost fish gilthead seabream. Moreover, cimetidine, which acts in vitro as an agonist of Hrh1 and Hrh2 on this species, was intraperitoneally injected in one and two years old gilthead seabream males. After three and five days of cimetidine injection, we found that this compound differently modified the gonadal hrs transcript levels and affects the testicular cell renewal and the gene expression of steroidogenesis-related molecules as well as the serum steroid levels. Our data point to cimetidine as a reproductive disruptor and elucidate a role for histamine in the gonad of this hermaphrodite fish species through Hr signalling.
authors.affiliation: Sección de Microscopía, Servicio de Apoyo a la Investigación, University of Murcia, Murcia 30100, Spain.
authors.forename: María
authors.initials: M
authors.lastname: García-García
authors.affiliation: Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, IMIB-Arrixaca, 30100 Murcia, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Liarte
authors.affiliation: Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, IMIB-Arrixaca, 30100 Murcia, Spain.
authors.forename: Nuria E
authors.initials: NE
authors.lastname: Gómez-González
authors.affiliation: Centro Oceanográfico de Murcia, Instituto Español de Oceanografía (IEO), Carretera de la Azohía s/n. Puerto de Mazarrón, 30860 Murcia, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: García-Alcázar
authors.affiliation: Nutrigenomics and Fish Growth Endocrinology Group, Institute of Aquaculture of Torre la Sal, IATS-CSIC, 12595 Ribera de Cabanes, Castellón, Spain.
authors.forename: Jaume
authors.initials: J
authors.lastname: Pérez-Sánchez
authors.affiliation: Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, IMIB-Arrixaca, 30100 Murcia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Meseguer
authors.affiliation: Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, IMIB-Arrixaca, 30100 Murcia, Spain.
authors.forename: Victoriano
authors.initials: V
authors.lastname: Mulero
authors.affiliation: Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, IMIB-Arrixaca, 30100 Murcia, Spain.
authors.forename: Alfonsa
authors.initials: A
authors.lastname: García-Ayala
authors.affiliation: Centro Oceanográfico de Murcia, Instituto Español de Oceanografía (IEO), Carretera de la Azohía s/n. Puerto de Mazarrón, 30860 Murcia, Spain. Electronic address: elena.chaves@mu.ieo.es.
authors.forename: Elena
authors.initials: E
authors.lastname: Chaves-Pozo
mesh_terms: D000818:Animals; D002927:Cimetidine / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D029941:Fish Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D057850:Hermaphroditic Organisms; D006635:Histamine H2 Antagonists / Q000633:toxicity*; D008297:Male; D011969:Receptors, Histamine H1 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011970:Receptors, Histamine H2 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018100:Receptors, Histamine H3 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects*; D021541:Sea Bream / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D013997:Time Factors
chemical_list: D052244:Endocrine Disruptors; D029941:Fish Proteins; D012739:Gonadal Steroid Hormones; D006635:Histamine H2 Antagonists; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D018100:Receptors, Histamine H3; D002927:Cimetidine
keywords: Cimetidine; Hermaphrodite fish; Histamine receptors; Testis