title: Characterization of Vibrio cholerae Strains Isolated from the Nigerian Cholera Outbreak in 2010.
abstract: We examined clinical samples from Nigerian patients with acute watery diarrhea for Vibrio cholerae during the 2010 cholera outbreak. A total of 109 suspected isolates were characterized, but only 57 V. cholerae strains could be confirmed using multiplex real-time PCR as well as rpoB sequencing and typed as V. cholerae O:1 Ogawa biotype El Tor. This finding highlighted the need for accurate diagnosis of cholera in epidemic countries to implement life-saving interventions.
authors.affiliation: Robert Koch Institute, Highly Pathogenic Microorganisms (ZBS 2), Berlin, Germany.
authors.forename: Susann
authors.initials: S
authors.lastname: Dupke
authors.affiliation: Nigerian Institute of Medical Research, Molecular Biology and Biotechnology, Yaba, Lagos, Nigeria.
authors.forename: Kehinde A
authors.initials: KA
authors.lastname: Akinsinde
authors.affiliation: Robert Koch Institute, Highly Pathogenic Microorganisms (ZBS 2), Berlin, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Grunow
authors.affiliation: Nigerian Institute of Medical Research, Molecular Biology and Biotechnology, Yaba, Lagos, Nigeria.
authors.forename: Bamidele A
authors.initials: BA
authors.lastname: Iwalokun
authors.affiliation: Danifol Biotechnology Consult, Onipanu, Lagos, Nigeria.
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Olukoya
authors.affiliation: Olabisi Onabanjo University, Sagamu, Ogun State, Nigeria.
authors.forename: Afolabi
authors.initials: A
authors.lastname: Oluwadun
authors.affiliation: Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany Fondation Congolaise pour la Recherche Medicale, Brazzaville, Republic of Congo.
authors.forename: Thirumalaisamy P
authors.initials: TP
authors.lastname: Velavan
authors.affiliation: Robert Koch Institute, Highly Pathogenic Microorganisms (ZBS 2), Berlin, Germany jacobd@rki.de.
authors.forename: Daniela
authors.initials: D
authors.lastname: Jacob
mesh_terms: D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology*; D003430:Cross-Sectional Studies; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004196:Disease Outbreaks; D005838:Genotype; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction; D009549:Nigeria / Q000453:epidemiology; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit