title: Neuroprotection induced by post-conditioning following ischemia/reperfusion in mice is associated with altered microRNA expression.
abstract: Ischemic preconditioning and ischemic postconditioning (IPostC) represent promising strategies to reduce ischemia-reperfusion (I/R) injury and attenuate the lethal ischemic damage following stroke. However, the mechanism underlying this attenuation remains to be elucidated. It was hypothesized that alterations in microRNA (miRNA) expression in the cerebral cortex and hippocampus of mice following I/R is associated with the functional improvement induced by IPostC. Behavioral changes were assessed in a mouse model of I/R in the absence or presence of IPostC, followed by microarray analyses to investigate the expressional alterations of miRNAs in the cerebral cortex and hippocampus of mice. The results of the present study revealed that IPostC abrogated the neurological impairment and hippocampus‑associated cognitive deficits induced by I/R, and upregulated or downregulated the expression levels of numerous miRNAs. Furthermore, the upregulation of miR‑19a, and the downregulation of miR‑1, let‑7f and miR‑124 expression levels following IPostC was confirmed utilizing reverse transcription‑quantitative polymerase chain reaction. The results of the present study demonstrated that alterations in miRNA expression in the cerebral cortex and hippocampus of mice following I/R was associated with the neuroprotection induced by IPostC.
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Miao
authors.affiliation: Department of Geratology, Psychiatric Hospital of Yunnan Kunming, Yunnan 650224, P.R. China.
authors.forename: Tian-Hao
authors.initials: TH
authors.lastname: Bao
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, P.R. China.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Han
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, P.R. China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yin
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, P.R. China.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism; D016000:Cluster Analysis; D003071:Cognition; D019295:Computational Biology / Q000379:methods; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000098:blood supply / Q000378:metabolism; D057775:Ischemic Postconditioning; D008297:Male; D018782:Maze Learning; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D000066829:Neuroprotection; D015427:Reperfusion Injury / Q000235:genetics / Q000503:physiopathology
chemical_list: D035683:MicroRNAs