title: Minocycline Loaded Hybrid Composites Nanoparticles for Mesenchymal Stem Cells Differentiation into Osteogenesis.
abstract: Bone transplants are used to treat fractures and increase new tissue development in bone tissue engineering. Grafting of massive implantations showing slow curing rate and results in cell death for poor vascularization. The potentials of biocomposite scaffolds to mimic extracellular matrix (ECM) and including new biomaterials could produce a better substitute for new bone tissue formation. A purpose of this study is to analyze polycaprolactone/silk fibroin/hyaluronic acid/minocycline hydrochloride (PCL/SF/HA/MH) nanoparticles initiate human mesenchymal stem cells (MSCs) proliferation and differentiation into osteogenesis. Electrospraying technique was used to develop PCL, PCL/SF, PCL/SF/HA and PCL/SF/HA/MH hybrid biocomposite nanoparticles and characterization was analyzed by field emission scanning electron microscope (FESEM), contact angle and Fourier transform infrared spectroscopy (FT-IR). The obtained results proved that the particle diameter and water contact angle obtained around 0.54 ± 0.12 to 3.2 ± 0.18 µm and 43.93 ± 10.8° to 133.1 ± 12.4° respectively. The cell proliferation and cell-nanoparticle interactions analyzed using (3-(4,5-dimethyl thiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt) MTS assay (Promega, Madison, WI, USA), FESEM for cell morphology and 5-Chloromethylfluorescein diacetate (CMFDA) dye for imaging live cells. Osteogenic differentiation was proved by expression of osteocalcin, alkaline phosphatase activity (ALP) and mineralization was confirmed by using alizarin red (ARS). The quantity of cells was considerably increased in PCL/SF/HA/MH nanoparticles when compare to all other biocomposite nanoparticles and the cell interaction was observed more on PCL/SF/HA/MH nanoparticles. The electrosprayed PCL/SF/HA/MH biocomposite nanoparticle significantly initiated increased cell proliferation, osteogenic differentiation and mineralization, which provide huge potential for bone tissue engineering.
authors.affiliation: Cellular and Molecular Epigenetics Lab, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore. THAM0115@e.ntu.edu.sg.
authors.forename: Allister Yingwei
authors.initials: AY
authors.lastname: Tham
authors.affiliation: Cellular and Molecular Epigenetics Lab, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore. CGandhimathi@ntu.edu.sg.
authors.forename: Chinnasamy
authors.initials: C
authors.lastname: Gandhimathi
authors.affiliation: Cellular and Molecular Epigenetics Lab, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore. jayaraman.p@ntu.edu.sg.
authors.forename: Jayaraman
authors.initials: J
authors.lastname: Praveena
authors.affiliation: Center for Nanofibers and Nanotechnology, Department of Mechanical Engineering, National University of Singapore, Singapore 119260, Singapore. mpejrv@nus.edu.sg.
authors.forename: Jayarama Reddy
authors.initials: JR
authors.lastname: Venugopal
authors.affiliation: Center for Nanofibers and Nanotechnology, Department of Mechanical Engineering, National University of Singapore, Singapore 119260, Singapore. seeram@nus.edu.sg.
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
authors.affiliation: Cellular and Molecular Epigenetics Lab, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore. dineshkumar@ntu.edu.sg.
authors.forename: Srinivasan Dinesh
authors.initials: SD
authors.lastname: Kumar
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000187:drug effects; D005349:Fibroins / Q000494:pharmacology; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008911:Minocycline / Q000008:administration & dosage / Q000494:pharmacology*; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000008:administration & dosage*; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters / Q000494:pharmacology; D047011:Silk / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000900:Anti-Bacterial Agents; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011091:Polyesters; D047011:Silk; C069306:5-chloromethylfluorescein; C016240:polycaprolactone; D005349:Fibroins; D008911:Minocycline
keywords: electrospraying; hyaluronic acid; minocycline hydrochloride; nanoparticles; polycaprolactone; silk fibroin