title: Differential diagnosis and molecular characterization of Hymenolepis nana and Hymenolepis diminuta (Cestoda: Cyclophyllidea: Hymenolepididae) based on nuclear rDNA ITS2 gene marker.
abstract: Given the widespread distribution and medical implication of members of the genus Hymenolepis, specific identification of the aetiological agent becomes imperative. For precise diagnosis of the species, molecular techniques such as PCR and RFLP of the nuclear ribosomal internal transcribed spacer 2 (rDNA-ITS2) gene marker were carried out. The results showed distinct restriction patterns for both Hymenolepis nana and Hymenolepis diminuta when digested with either of the enzymes RsaI, HaeIII or HhaI. The annotated rDNA-ITS2 sequences from the two species revealed differences in the length; the folded secondary structure also depicted clear demarcation between the two species with variations in length of the helices, pyrimidine-pyrimidine mismatches and sites where motifs occur. In phylogenetic analysis of the evolutionary relationship between the two species as well as with other members of the family Hymenolepididae, the species causing human hymenolepiasis were found to be distantly related as they diverged independently from the ancestral lineage.
authors.affiliation: Department of Zoology, North-Eastern Hill University, Shillong, 793022, Meghalaya, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Zoology, North-Eastern Hill University, Shillong, 793022, Meghalaya, India.
authors.forename: Damanbha
authors.initials: D
authors.lastname: Lyngdoh
authors.affiliation: Department of Zoology, North-Eastern Hill University, Shillong, 793022, Meghalaya, India.
authors.forename: Bishnupada
authors.initials: B
authors.lastname: Roy
authors.affiliation: Department of Zoology, North-Eastern Hill University, Shillong, 793022, Meghalaya, India. tandonveena@gmail.com.|Biotech Park, Lucknow, 226021, Uttar Pradesh, India. tandonveena@gmail.com.
authors.forename: Veena
authors.initials: V
authors.lastname: Tandon
mesh_terms: D000818:Animals; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D003937:Diagnosis, Differential; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D006925:Hymenolepiasis / Q000175:diagnosis* / Q000469:parasitology; D048190:Hymenolepis diminuta / Q000235:genetics*; D048189:Hymenolepis nana / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats
chemical_list: D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers
keywords: Cestodes; Hymenolepiasis; Hymenolepis diminuta; Hymenolepis nana; rDNA ITS2