title: The Genome of Undifilum oxytropis Provides Insights into Swainsonine Biosynthesis and Locoism.
abstract: Undifilum oxytropis is a fungal endophyte of locoweeds. It produces swainsonine, which is the principal toxic ingredient of locoweeds. However, the genes, pathways and mechanisms of swainsonine biosynthesis are not known. In this study, the genome of U. oxytropis was firstly sequenced and assembled into a 70.05 megabases (Mb) draft genome, which encoded 11,057 protein-coding genes, and 54% of them were similar to current publicly available sequences. U. oxytropis genes were annotated and 164 putative genes were annotated into enzymes, such as Saccharopine dehydrogenase, Saccharopine oxidase, and Pyrroline-5-carboxylate reductase, hypothesized to be involved in the biosynthesis pathway of swainsonine. The genome sequence and gene annotation of U. oxytropis will provide new insights into functional analyses. The characterization of genes in swainsonine biosynthesis will greatly facilitate locoweed poisoning research and help direct locoism management.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Lu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Haiyun
authors.initials: H
authors.lastname: Quan
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Zhenhui
authors.initials: Z
authors.lastname: Ren
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Ruixu
authors.initials: R
authors.lastname: Xue
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Baoyu
authors.initials: B
authors.lastname: Zhao
mesh_terms: D001203:Ascomycota / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D016681:Genome, Fungal / Q000502:physiology*; D017026:Swainsonine / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D017026:Swainsonine