title: Red-Green-Blue Trichromophoric Nanoparticles with Dual Fluorescence Resonance Energy Transfer: Highly Sensitive Fluorogenic Response Toward Polyanions.
abstract: A red-green-blue (RGB) trichromophoric fluorescent organic nanoparticle exhibiting multi-colour emission was constructed; the blue-emitting cationic oligofluorene nanoparticle acted as an energy-donor scaffold to undergo fluorescence resonance energy transfer (FRET) to a red-emitting dye embedded in the nanoparticle (interior FRET) and to a green-emitting dye adsorbed on the surface through electrostatic interactions (exterior FRET). Each FRET event occurs independently and is free from sequential FRET, thus the resultant dual-FRET system exhibits multi-colour emission, including white, in aqueous solution and film state. A characteristic white-emissive nanoparticle showed visible responses upon perturbation of the exterior FRET efficiency by acceptor displacement, leading to highly sensitive responses toward polyanions in a ratiometric manner. Specifically, our system exhibits high sensitivity toward heparin with an extremely low detection limit.
authors.affiliation: Molecular Design & Function Group, National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba, Ibaraki, 305-0047, Japan.|Department of Materials Science and Engineering, Graduate School of Pure and Applied Science, University of Tsukuba, 1-1-1, Tennoudai, Tsukuba, Ibaraki, 305-8571, Japan.
authors.forename: Jinjia
authors.initials: J
authors.lastname: Xu
authors.affiliation: Molecular Design & Function Group, National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba, Ibaraki, 305-0047, Japan.
authors.forename: Atsuro
authors.initials: A
authors.lastname: Takai
authors.affiliation: Molecular Design & Function Group, National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba, Ibaraki, 305-0047, Japan. TAKEUCHI.Masayuki@nims.go.jp.|Department of Materials Science and Engineering, Graduate School of Pure and Applied Science, University of Tsukuba, 1-1-1, Tennoudai, Tsukuba, Ibaraki, 305-8571, Japan. TAKEUCHI.Masayuki@nims.go.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Takeuchi
mesh_terms: D000838:Anions / Q000737:chemistry; D003116:Color; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010569:Perylene / Q000031:analogs & derivatives / Q000737:chemistry; D011714:Pyrans / Q000737:chemistry; D012680:Sensitivity and Specificity; D013343:Styrenes / Q000737:chemistry; D014867:Water
chemical_list: C560550:3,4,9,10-perylene tetracarboxylic acid; D000838:Anions; D005456:Fluorescent Dyes; D011714:Pyrans; D013343:Styrenes; D014867:Water; D010569:Perylene; D006493:Heparin; C082825:4-(dicyanomethylene)-2-methyl-6-(4-(dimethylamino)styryl)-4H-pyran
keywords: FRET; biosensors; multi-colour emission; nanoparticles; supramolecular assembly