title: Detection of Interleukin-1β and Interleukin-6 in Human Multiple Myeloma by Fluorescent in situ Hybridization.
abstract: Using fluorescent in situ hybridization together with cell surface marker staining, we studied the expression of mRNA of IL-6 and mRNA of IL-1β in bone marrow samples from human multiple myeloma patients. It is known that IL-6 can stimulate B cell growth and differentiation and recently it has been suggested that IL-6 is responsible for autocrine growth stimulation of myeloma cells and that IL-1 may play a role in bone resorption. These interleukins have previously been detected in the supernatants of cultured myeloma cells. Here we report the expression of IL-1β mRNA by plasma cells, T cells and macrophages according to morphology and immunologic marker analysis, suggesting that not only myeloma cells but other cell types can also contribute to the production of IL-1β and thus to bone-resorption. IL-6 mRNA could not be detected in plasma cells from bone marrow aspirates but were present in monocytes and T cells, suggesting that in vivo IL-6 stimulates the growth of myeloma cells in a paracrine instead of an autocrine way.
authors.affiliation: a Department of Immunology, Erasmus University Rotterdam and Academic Hospital, Rotterdam-Dijkzigt, Holland.
authors.forename: M H
authors.initials: MH
authors.lastname: Bakkus
authors.affiliation: a Department of Immunology, Erasmus University Rotterdam and Academic Hospital, Rotterdam-Dijkzigt, Holland.
authors.forename: K M
authors.initials: KM
authors.lastname: Brakel-Van Peer
authors.affiliation: a Department of Immunology, Erasmus University Rotterdam and Academic Hospital, Rotterdam-Dijkzigt, Holland.
authors.forename: H J
authors.initials: HJ
authors.lastname: Adriaansen
authors.affiliation: a Department of Immunology, Erasmus University Rotterdam and Academic Hospital, Rotterdam-Dijkzigt, Holland.
authors.forename: T W
authors.initials: TW
authors.lastname: Van Den Akker
authors.affiliation: a Department of Immunology, Erasmus University Rotterdam and Academic Hospital, Rotterdam-Dijkzigt, Holland.
authors.forename: R
authors.initials: R
authors.lastname: Benner
keywords: IL-1β; IL-6; in situ hybridization; multiple myeloma