title: Strawberry tannins inhibit IL-8 secretion in a cell model of gastric inflammation.
abstract: In the present study we chemically profiled tannin-enriched extracts from strawberries and tested their biological properties in a cell model of gastric inflammation. The chemical and biological features of strawberry tannins after in vitro simulated gastric digestion were investigated as well. The anti-inflammatory activities of pure strawberry tannins were assayed to get mechanistic insights. Tannin-enriched extracts from strawberries inhibit IL-8 secretion in TNFα-treated human gastric epithelial cells by dampening the NF-κB signaling. In vitro simulated gastric digestion slightly affected the chemical composition and the biological properties of strawberry tannins. By using pure compounds, we found that casuarictin may act as a pure NF-κB inhibitor while agrimoniin inhibits IL-8 secretion also acting on other biological targets; in our system procyanidin B1 prevents the TNFα-induced effects without interfering with the NF-κB pathway. We conclude that strawberry tannins, even after in vitro simulated gastric digestion, exert anti-inflammatory activities at nutritionally relevant concentrations.
authors.affiliation: Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Fumagalli
authors.affiliation: Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Sangiovanni
authors.affiliation: Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all⿿Adige, Italy.
authors.forename: Urska
authors.initials: U
authors.lastname: Vrhovsek
authors.affiliation: Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Piazza
authors.affiliation: Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Colombo
authors.affiliation: Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all⿿Adige, Italy.
authors.forename: Mattia
authors.initials: M
authors.lastname: Gasperotti
authors.affiliation: Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all⿿Adige, Italy.
authors.forename: Fulvio
authors.initials: F
authors.lastname: Mattivi
authors.affiliation: Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Italy.
authors.forename: Emma
authors.initials: E
authors.lastname: De Fabiani
authors.affiliation: Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Italy. Electronic address: mario.dellagli@unimi.it.
authors.forename: Mario
authors.initials: M
authors.lastname: Dell'Agli
mesh_terms: D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D031985:Fragaria / Q000737:chemistry*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D005756:Gastritis / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D011401:Promoter Regions, Genetic; D015398:Signal Transduction / Q000187:drug effects; D013634:Tannins / Q000302:isolation & purification / Q000494:pharmacology*; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; C508606:CXCL8 protein, human; D016209:Interleukin-8; D016328:NF-kappa B; D010936:Plant Extracts; D013634:Tannins; D014409:Tumor Necrosis Factor-alpha
keywords: Agrimoniin; Casuarictin; Ellagic acid; Ellagitannins; Fragaria spp; Gastric inflammation; IL-8; Procyanidin B1; Procyanidins; Strawberry