title: Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cells.
abstract: Respiratory syncytial virus (RSV) is one of the leading causes of range of symptoms from mild upper to serious lower respiratory virus infections in infants, immunocompromised individuals, and the elderly. Despite many decades of research and development, a licensed RSV vaccine is not available for use in human. Since the RSV F and G proteins induce neutralizing antibodies and confer protection from infection, they are important for understanding disease and for developing vaccines and access to purified, expressed proteins is important to RSV research and diagnostics. We describe methods to produce recombinant RSV F and G proteins in human cells and purify these proteins using Ni Sepharose affinity chromatography.
authors.affiliation: Department of Pediatrics, School of Medicine, Emory University and Childrens Healthcare of Atlanta, 2015 UppergateDr, Atlanta, GA, 30322, USA.
authors.forename: Samadhan J
authors.initials: SJ
authors.lastname: Jadhao
authors.affiliation: Department of Pediatrics, School of Medicine, Emory University and Childrens Healthcare of Atlanta, 2015 UppergateDr, Atlanta, GA, 30322, USA. larry.anderson@emory.edu.
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Anderson
mesh_terms: D045744:Cell Line, Tumor; D002846:Chromatography, Affinity; D006801:Humans; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D018357:Respiratory Syncytial Virus Infections / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D014759:Viral Envelope Proteins
keywords: Expression; F protein; G protein; Human cell; Respiratory syncytial virus; Western blot