title: A high-throughput in vitro ring assay for vasoactivity using magnetic 3D bioprinting.
abstract: Vasoactive liabilities are typically assayed using wire myography, which is limited by its high cost and low throughput. To meet the demand for higher throughput in vitro alternatives, this study introduces a magnetic 3D bioprinting-based vasoactivity assay. The principle behind this assay is the magnetic printing of vascular smooth muscle cells into 3D rings that functionally represent blood vessel segments, whose contraction can be altered by vasodilators and vasoconstrictors. A cost-effective imaging modality employing a mobile device is used to capture contraction with high throughput. The goal of this study was to validate ring contraction as a measure of vasoactivity, using a small panel of known vasoactive drugs. In vitro responses of the rings matched outcomes predicted by in vivo pharmacology, and were supported by immunohistochemistry. Altogether, this ring assay robustly models vasoactivity, which could meet the need for higher throughput in vitro alternatives.
authors.affiliation: Nano3D Biosciences, Houston, TX 77030, USA.
authors.forename: Hubert
authors.initials: H
authors.lastname: Tseng
authors.affiliation: Nano3D Biosciences, Houston, TX 77030, USA.
authors.forename: Jacob A
authors.initials: JA
authors.lastname: Gage
authors.affiliation: Nano3D Biosciences, Houston, TX 77030, USA.
authors.forename: William L
authors.initials: WL
authors.lastname: Haisler
authors.affiliation: Nano3D Biosciences, Houston, TX 77030, USA.
authors.forename: Shane K
authors.initials: SK
authors.lastname: Neeley
authors.affiliation: LC Sciences, Houston, TX 77030, USA.
authors.forename: Tsaiwei
authors.initials: T
authors.lastname: Shen
authors.affiliation: LC Sciences, Houston, TX 77030, USA.
authors.forename: Chris
authors.initials: C
authors.lastname: Hebel
authors.affiliation: AstraZeneca, Waltham, MA 02451, USA.
authors.forename: Herbert G
authors.initials: HG
authors.lastname: Barthlow
authors.affiliation: AstraZeneca, Waltham, MA 02451, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Wagoner
authors.affiliation: Nano3D Biosciences, Houston, TX 77030, USA.
authors.forename: Glauco R
authors.initials: GR
authors.lastname: Souza
mesh_terms: D062028:Bioprinting / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D057166:High-Throughput Screening Assays; D006801:Humans; D008280:Magnetics; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000502:physiology; D014662:Vasoconstrictor Agents / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D014662:Vasoconstrictor Agents