title: Complete Sequence of pEC012, a Multidrug-Resistant IncI1 ST71 Plasmid Carrying bla CTX-M-65, rmtB, fosA3, floR, and oqxAB in an Avian Escherichia coli ST117 Strain.
abstract: A 139,622-bp IncI1 ST71 conjugative plasmid pEC012 from an avian Escherichia coli D-ST117 strain was sequenced, which carried five IS26-bracketed resistance modules: IS26-fosA3-orf1-orf2-Δorf3-IS26, IS26-fip-ΔISEcp1-bla CTX-M-65-IS903D-iroN-IS26, IS26-ΔtnpR-bla TEM-1-rmtB-IS26, IS26-oqxAB-IS26, and IS26-floR-aac(3)-IV-IS26. The backbone of pEC012 was similar to that of several other IncI1 ST71 plasmids: pV408, pM105, and pC271, but these plasmids had different arrangements of multidrug resistance region. In addition, the novel ISEc57 element was identified, which is in the IS21 family. The stepwise emergence of multi-resistance regions demonstrated the accumulation of different resistance determinants through homologous recombination. To the best of our knowledge, this is the first study to identify a multidrug-resistant IncI1 ST71 plasmid carrying bla CTX-M-65, rmtB, fosA3, floR, and oqxAB in an avian E. coli ST117 strain.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University Zhengzhou, China.
authors.forename: Yu-Shan
authors.initials: YS
authors.lastname: Pan
authors.affiliation: West China Hospital, Sichuan University Chengdu, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zong
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University Zhengzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University Zhengzhou, China.
authors.forename: Xiang-Dang
authors.initials: XD
authors.lastname: Du
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University Zhengzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University Zhengzhou, China.
authors.forename: Xing-Hao
authors.initials: XH
authors.lastname: Zhong
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University Zhengzhou, China.
authors.forename: Gong-Zheng
authors.initials: GZ
authors.lastname: Hu
keywords: 16S rRNA methylase; IS26-composite transposon; Tn2; extended-spectrum β-lactamases; fosfomycin