title: Separation of high-purity syringol and acetosyringone from rice straw-derived bio-oil by combining the basification-acidification process and column chromatography.
abstract: Numerous technologies have been used to reclaim valuable chemicals from bio-oil. In this study, a combination of the basification-acidification process and column chromatography was employed for the separation of high-purity syringol and acetosyringone from rice straw-derived bio-oil. The optimal conditions for the basification-acidification process and the possible precipitation mechanism of the basification were explored. The results showed the following as the optimal conditions for the basification process: mass ratio of calcium hydroxide (Ca(OH)2) to bio-oil, 2.0; reaction temperature, 70°C; and reaction time, 30 min. The results also showed that 1.6 mol of hydrochloric acid (HCl) per gram of bio-oil was optimal for the acidification. The precipitation was found to proceed via a possible mechanism involving the reaction of the phenolic compounds in the bio-oil with Ca(OH)2to produce a precipitate. After further separation by column chromatography, purities of 91.4 and 96.2% (from gas chromatography-mass spectrometry) were obtained for syringol and acetosyringone, respectively. Their recoveries for the whole process were 73.0 and 39.3%, respectively.
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai, P. R. China.
authors.forename: Shilai
authors.initials: S
authors.lastname: Hao
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai, P. R. China.
authors.forename: Kaifei
authors.initials: K
authors.lastname: Chen
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai, P. R. China.
authors.forename: Leichang
authors.initials: L
authors.lastname: Cao
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai, P. R. China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Luo
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai, P. R. China. zhangsc@fudan.edu.cn.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai, P. R. China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Chen
mesh_terms: D000098:Acetophenones / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D056804:Biofuels / Q000032:analysis*; D002126:Calcium Hydroxide / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006358:Hot Temperature; D006851:Hydrochloric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D012275:Oryza / Q000737:chemistry*; D011748:Pyrogallol / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000098:Acetophenones; D056804:Biofuels; D011748:Pyrogallol; C010120:pyrogallol 1,3-dimethyl ether; C051667:acetosyringone; D002126:Calcium Hydroxide; D006851:Hydrochloric Acid
keywords: Acetosyringone; Basification-acidification; Bio-oil; Column chromatography; Syringol