title: Small molecule perturbations of septins.
abstract: Progress on the study of the molecular and cellular biology of septins would be greatly accelerated by the development of small molecules that directly inhibit higher-order septin assembly in vivo. By comparison, molecules like latrunculin, paclitaxil, benomyl, etc. allow researchers to acutely perturb the actin or tubulin cytoskeletal networks. Two small molecules, forchlorfenuron (FCF; N-(2-chloro-4pyridyl)-N-phenylurea) and 1-ethyl-3-(4-methoxyphenyl)-6-methylpyrimido[5,4-e][1,2,4]triazine-5,7-dione (PubChem CID 906558), have documented effects on septin localization and/or function, although for each molecule there is also strong evidence for off-target effects. In this chapter we provide a summary of ways to utilize FCF to alter higher-order septin assembly properties in living cells.
authors.affiliation: University of Colorado Anschutz Medical Campus, Aurora, CO, United States.
authors.forename: L R
authors.initials: LR
authors.lastname: Heasley
authors.affiliation: University of Colorado Anschutz Medical Campus, Aurora, CO, United States.
authors.forename: M A
authors.initials: MA
authors.lastname: McMurray
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000302:isolation & purification; D003599:Cytoskeleton / Q000737:chemistry / Q000187:drug effects; D006801:Humans; D046912:Multiprotein Complexes / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification; D010671:Phenylurea Compounds / Q000737:chemistry / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D011725:Pyridines / Q000737:chemistry / Q000494:pharmacology*; D058112:Septins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000199:Actins; D046912:Multiprotein Complexes; D010671:Phenylurea Compounds; D011725:Pyridines; D058112:Septins; C116093:N-(2-chloro-4-pyridyl)-N'-phenylurea
keywords: Drug; Forchlorfenuron; GTP; Molecule; Off-target; Perturbations