title: [A preliminary analysis on potentially targeted genes of induced chemotherapy in supraglottic laryngeal squamous cell carcinoma].
abstract: OBJECTIVE
  To analyze the differentially expressed genes produced by paclitaxel, cisplatin and 5-fluorouracil(TPF) regimen induction chemotherapy and the potentially functionally targeted genes of the induced chemotherapy in supraglottic laryngeal squamous cell carcinoma.
  
  
  METHODS
  A total of 11 tissue samples from patients diagnosed as supraglottic carcinoma who didn't receive any treatment before were analyzed with microarray. The patients were divided into two groups based on their responses to the induction chemotherapy: 7 were sensitive to chemotherapy and 4 were non-sensitive. Gene expressions were detected by Illumina Human HT-12 BeadChip. The bioinformatics analysis online was used to analyze the differentially expressed genes.
  
  
  RESULTS
  A total of 1 554 differentially expressed genes related to chemosensitivity were found. Analyzed with GO database, the up-regulated genes included the functional sets of biological adhesion, immune system development and stem cell proliferation, and the down-regulated genes included the functional sets of cell junction organization, phosphorus metabolic process and cell morphogenesis involved in differentiation. Analyzed with KEGG database, the up-regulated pathways included p53, cell adhesion and Ras signaling pathways, and the down-regulated pathways included focal adhesion, endocytosis and ErbB signaling pathways. There were statistically significant differences in the expressions of MAPK10, PIK3R5 and JUN genes, which had biological significance, between sensitive patients and non-sensitive patients.
  
  
  CONCLUSIONS
  MAPK10, PIK3R5 and JUN may be considered as potentially functional genes of the induced chemotherapy in supraglottic carcinoma.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology Head and Neck Surgery Ministry of Education (Capital Medical University), Key Laboratory of Molecular Pathology of Head and Neck of Beijing, Beijing 100730, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology Head and Neck Surgery Ministry of Education (Capital Medical University), Key Laboratory of Molecular Pathology of Head and Neck of Beijing, Beijing 100730, China.
authors.forename: M
authors.initials: M
authors.lastname: Lian
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology Head and Neck Surgery Ministry of Education (Capital Medical University), Key Laboratory of Molecular Pathology of Head and Neck of Beijing, Beijing 100730, China.
authors.forename: J G
authors.initials: JG
authors.lastname: Fang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology Head and Neck Surgery Ministry of Education (Capital Medical University), Key Laboratory of Molecular Pathology of Head and Neck of Beijing, Beijing 100730, China.
authors.forename: H G
authors.initials: HG
authors.lastname: Liu
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology Head and Neck Surgery Ministry of Education (Capital Medical University), Key Laboratory of Molecular Pathology of Head and Neck of Beijing, Beijing 100730, China.
authors.forename: L Z
authors.initials: LZ
authors.lastname: Meng
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology Head and Neck Surgery Ministry of Education (Capital Medical University), Key Laboratory of Molecular Pathology of Head and Neck of Beijing, Beijing 100730, China.
authors.forename: H Z
authors.initials: HZ
authors.lastname: Ma
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital of Capital Medical University, Key Laboratory of Otorhinolaryngology Head and Neck Surgery Ministry of Education (Capital Medical University), Key Laboratory of Molecular Pathology of Head and Neck of Beijing, Beijing 100730, China.
authors.forename: L
authors.initials: L
authors.lastname: Feng
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000235:genetics*; D002945:Cisplatin / Q000008:administration & dosage; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005472:Fluorouracil / Q000008:administration & dosage; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D016158:Genes, p53; D006801:Humans; D060828:Induction Chemotherapy; D007822:Laryngeal Neoplasms / Q000188:drug therapy* / Q000235:genetics*; D017239:Paclitaxel / Q000008:administration & dosage; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D016283:Proto-Oncogene Proteins p21(ras); D017239:Paclitaxel; D002945:Cisplatin; D005472:Fluorouracil