title: Impact of shear stress on Src and focal adhesion kinase phosphorylation in fibrinogen-adherent platelets.
abstract: : Shear stress alone can activate platelets resulting in a subsequent platelet aggregation, so-called 'shear-induced platelet aggregation'. In our work, we analyzed how differently elevated shear stress impacts the Src and focal adhesion kinase (FAK) activation in fibrinogen-adherent human platelets. We detected the extents of Src pY418 and FAK pY397 activations in platelets on immobilized fibrinogen and over BSA under shear conditions. Moreover, we analyzed the role of αIIbβ3 in the shear-induced platelet signaling by performing our experiments in the presence of the αIIbβ3-antagonist Abciximab. Abnormally high shear rates (5000 s) significantly increased the extent of phosphorylation of both tyrosine kinases after short (2 min) incubation time independently of the presence or absence of the integrin αIIbβ3 ligand, fibrinogen. We could see considerably greater Src activation on immobilized fibrinogen than on BSA, but the extent of FAK Y397 phosphorylation was independent on the matrix. Abciximab not only reduced the Src and FAK signaling in platelets exposed to 5000 s on immobilized fibrinogen, but in platelets exposed to 5000 s over BSA as well. Our data indicate that whereas Src activation under shear stress is dominantly ligand-dependent, FAK signaling seems to be mostly shear induced.
authors.affiliation: aBiomedical Engineering Department, International University, Vietnam National University, Ho Chi Minh City, Vietnam bDepartment of Hemostasis, Hemotherapy, and Transfusion Medicine, Heinrich Heine University Medical Center, Düsseldorf, Germany.
authors.forename: Khon C
authors.initials: KC
authors.lastname: Huynh
authors.forename: Marianna
authors.initials: M
authors.lastname: Gyenes
authors.forename: Thi-Hiep
authors.initials: TH
authors.lastname: Nguyen
authors.forename: Toi Van
authors.initials: TV
authors.lastname: Vo
authors.forename: Volker R
authors.initials: VR
authors.lastname: Stoldt
mesh_terms: D000077284:Abciximab; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000925:Anticoagulants / Q000494:pharmacology; D001665:Binding Sites; D001696:Biomechanical Phenomena; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D004789:Enzyme Activation; D005340:Fibrinogen / Q000378:metabolism*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology; D010766:Phosphorylation; D010973:Platelet Adhesiveness; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013314:Stress, Mechanical
chemical_list: D000911:Antibodies, Monoclonal; D000925:Anticoagulants; D007140:Immunoglobulin Fab Fragments; D005340:Fibrinogen; D051416:Focal Adhesion Protein-Tyrosine Kinases; D016392:Proto-Oncogene Proteins pp60(c-src); D000077284:Abciximab