title: Efficient TALEN-mediated myostatin gene editing in goats.
abstract: Myostatin (MSTN) encodes a negative regulator of skeletal muscle mass that might have applications for promoting muscle growth in livestock. In this study, we aimed to test whether targeted MSTN editing, mediated by transcription activator-like effector nucleases (TALENs), is a viable approach to create myostatin-modified goats (Capra hircus).
  
  
  
  We obtained a pair of TALENs (MTAL-2) that could recognize and cut the targeted MSTN site in the goat genome. Fibroblasts from pedigreed goats were co-transfected with MTAL-2, and 272 monoclonal cell strains were confirmed to have mono- or bi-allelic mutations in MSTN. Ten cell strains with different genotypes were used as donor cells for somatic cell nuclear transfer, which produced three cloned kids (K179/MSTN(-/-), K52-2/MSTN (+/-), and K52-1/MSTN (+/+)).
  
  
  
  The results suggested that the MTAL-2 could disrupt MSTN efficiently in the goat genome. The mutated somatic cells could be used to produce MSTN-site mutated goats without developmental disruption. Thus, TALENs is an effective method for accurate genome editing to produce site-modified goats.
authors.affiliation: College of Veterinary Medicine, Yangzhou University, No. 12 Wenhui Road, Yangzhou, 225009, Jiangsu Province, People's Republic of China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, Yangzhou University, No. 12 Wenhui Road, Yangzhou, 225009, Jiangsu Province, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Lu
authors.affiliation: College of Veterinary Medicine, Yangzhou University, No. 12 Wenhui Road, Yangzhou, 225009, Jiangsu Province, People's Republic of China.
authors.forename: Yuguo
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: College of Veterinary Medicine, Yangzhou University, No. 12 Wenhui Road, Yangzhou, 225009, Jiangsu Province, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Yangzhou University, No. 12 Wenhui Road, Yangzhou, 225009, Jiangsu Province, People's Republic of China.
authors.forename: Shaozheng
authors.initials: S
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Yangzhou University, No. 12 Wenhui Road, Yangzhou, 225009, Jiangsu Province, People's Republic of China.
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Qi
authors.affiliation: College of Veterinary Medicine, Yangzhou University, No. 12 Wenhui Road, Yangzhou, 225009, Jiangsu Province, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shao
authors.affiliation: College of Veterinary Medicine, Yangzhou University, No. 12 Wenhui Road, Yangzhou, 225009, Jiangsu Province, People's Republic of China.
authors.forename: Mengmin
authors.initials: M
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Yangzhou University, No. 12 Wenhui Road, Yangzhou, 225009, Jiangsu Province, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Mi
authors.affiliation: College of Veterinary Medicine, Yangzhou University, No. 12 Wenhui Road, Yangzhou, 225009, Jiangsu Province, People's Republic of China. chengyong@yzu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000473:pathology*; D000072669:Gene Editing; D016678:Genome; D006041:Goats; D008297:Male; D016297:Mutagenesis, Site-Directed; D055435:Myostatin / Q000235:genetics*; D000069896:Transcription Activator-Like Effector Nucleases / Q000378:metabolism*
chemical_list: D055435:Myostatin; D000069896:Transcription Activator-Like Effector Nucleases
keywords: Muscularity; Mutational goat; Myostatin; Nuclear transfer; TALENs