title: Controlled release hydrogen sulfide delivery system based on mesoporous silica nanoparticles protects graft endothelium from ischemia-reperfusion injury.
abstract: Hydrogen sulfide (H2S) functions as a protective gas transmitter in various physiological and pathological processes, but the lack of ideal donors severely hampers the clinical application of H2S. This study aims to construct a controlled release H2S donor and evaluate its protective effect on graft endothelium. Mesoporous silica nanoparticles (MSNs) were synthesized using the sol-gel method and loaded with diallyl trisulfide (DATS), an H2S-releasing agent named DATS-MSN. In vitro experiments showed that DATS-MSN could alleviate endothelial cell inflammation and enhance endothelial cell proliferation and migration. In vivo experiments demonstrated that the apoptosis of graft endothelium was mitigated in the presence of DATS-MSN. Our results indicated that DATS-MSN, releasing H2S in a controlled release fashion, could serve as an ideal H2S donor.
authors.affiliation: Department of Cardiac Surgery, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital.
authors.forename: Wenshuo
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Cardiac Surgery, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital; Department of Cardiothoracic Surgery, Huashan Hospital, Fudan University.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University.
authors.forename: Huili
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cardiac Surgery, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital.
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers; Department of Macromolecular Science, Fudan University, Shanghai, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers; Department of Macromolecular Science, Fudan University, Shanghai, People's Republic of China.
authors.forename: Wuli
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Cardiac Surgery, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital.
authors.forename: Changfa
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Cardiac Surgery, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Wang
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D006862:Hydrogen Sulfide / Q000494:pharmacology / Q000627:therapeutic use*; D020287:In Situ Nick-End Labeling; D007249:Inflammation / Q000473:pathology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008807:Mice, Inbred BALB C; D050997:Nanomedicine; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D020011:Protective Agents; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000517:prevention & control*; D012822:Silicon Dioxide / Q000737:chemistry*; D013440:Sulfides / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000498:Allyl Compounds; D003692:Delayed-Action Preparations; D020011:Protective Agents; D013440:Sulfides; C042577:diallyl trisulfide; D012822:Silicon Dioxide; D006862:Hydrogen Sulfide
keywords: cardiac allograft vasculopathy; cellular uptake; inflammatory response; proliferation; rejection