title: HDS: Establishment of a New Cell Line from a Hairy Cell Leukemia Patient with Resistance to Alpha-Interferon Therapy.
abstract: A new cell line, designated "HDS", was established in a suspension culture derived from the peripheral blood of a patient with hairy cell leukemia (HCL) who developed clinical resistance to alpha-interferon (aIFN) therapy. The patient exhibited a clinical picture characteristic of HCL, including splenomegaly, cytopenias, and tartrate-resistant acid phosphatase (TRAP)-positive "hairy" cells in blood and marrow. Chromosomal studies revealed that the cultured cells possess the chromosomal abnormality +12. Cytochemical and immunologic studies show the HDS cell line had the phenotype of a B-lymphocyte. HDS cells expressed the HLA-DR and CD19 surface antigens, but were negative for early B (CD10) and T (CD2, CD3) cell markers. The cells are also negative for other T-cell, granulocytic and monocytic markers and for typical HCL markers such as CD11c and CD22. However, the expression of these antigens was induced by in-vitro treatment of the cells with the differentiation-inducing agent tetradecanoyl phorbol acetate (TPA). Ultrastructural analyses of the cultured cells revealed a display of surface microvilli mixed with ruffles in a classical hairy cell pattern. It is therefore highly likely that the HDS cells represent HCL cells in an atypical stage of differentiation.
authors.affiliation: a Section of Hematology/Oncology, Department of Medicine, University of Chicago Medical Center, Chicago, Illinois, USA.
authors.forename: S
authors.initials: S
authors.lastname: Vedantham
authors.affiliation: b Section of Hematology, Department of Medicine, Second Teaching Hospital, Xi'an Medical University, Xi'an, Shaanxi, The People's Republic of China.
authors.forename: S H
authors.initials: SH
authors.lastname: Wu
authors.affiliation: a Section of Hematology/Oncology, Department of Medicine, University of Chicago Medical Center, Chicago, Illinois, USA.
authors.forename: D
authors.initials: D
authors.lastname: Gurfel
authors.affiliation: a Section of Hematology/Oncology, Department of Medicine, University of Chicago Medical Center, Chicago, Illinois, USA.
authors.forename: R
authors.initials: R
authors.lastname: Lovis
authors.affiliation: a Section of Hematology/Oncology, Department of Medicine, University of Chicago Medical Center, Chicago, Illinois, USA.
authors.forename: H M
authors.initials: HM
authors.lastname: Golomb
authors.affiliation: a Section of Hematology/Oncology, Department of Medicine, University of Chicago Medical Center, Chicago, Illinois, USA.
authors.forename: H
authors.initials: H
authors.lastname: Gamliel
keywords: Hairy cell; TPA; cell line; drug resistance; immunolabeling; interferon; leukemia