title: Label-free cellular structure imaging with 82 nm lateral resolution using an electron-beam excitation-assisted optical microscope.
abstract: We present label-free and high spatial-resolution imaging for specific cellular structures using an electron-beam excitation-assisted optical microscope (EXA microscope). Images of the actin filament and mitochondria of stained HeLa cells, obtained by fluorescence and EXA microscopy, were compared to identify cellular structures. Based on these results, we demonstrated the feasibility of identifying label-free cellular structures at a spatial resolution of 82 nm. Using numerical analysis, we calculated the imaging depth region and determined the spot size of a cathodoluminescent (CL) light source to be 83 nm at the membrane surface.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fukuta
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Masuda
authors.forename: Wataru
authors.initials: W
authors.lastname: Inami
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Kawata