title: Dysregulated function of normal human epidermal keratinocytes in the absence of filaggrin.
abstract: The aim of the present study was to investigate the impact of filaggrin knockdown on the function of normal human epidermal keratinocytes (NHEKs). Filaggrin expression levels in NHEKs were knocked down by lentivirus (LV) encoding small hairpin RNA (shRNA), with control cells infected with nonsense shRNA or not infected. Cell migration and invasion were assayed using Transwell inserts, cell adhesion and proliferation by the Cell Counting kit‑8 assay, and apoptosis and cell cycle progression by flow cytometry. shRNA efficiently suppressed expression of filaggrin protein. The LV group had significantly decreased cell migration, adhesion and proliferation, and increased apoptosis compared with the control groups (P=0.027). In addition, the proportion of cells in G1 and G2 phases were significantly increased in the LV group compared with control groups (P=0.018). The results of the present study demonstrate that filaggrin knockdown inhibits NHEK migration, adhesion and proliferation, promotes apoptosis and disturbs cell cycle progression.
authors.affiliation: Department of Dermatology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Dang
authors.affiliation: Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Dermatology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China.
authors.forename: Xianguang
authors.initials: X
authors.lastname: Meng
authors.affiliation: College of Life Science, Shandong Normal University, Jinan, Shandong 250014, P.R. China.
authors.forename: Liguo
authors.initials: L
authors.lastname: An
authors.affiliation: Department of Endocrinology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Pang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002448:Cell Adhesion / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D000078404:Epidermal Cells; D000091344:Filaggrin Proteins; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006801:Humans; D007381:Intermediate Filament Proteins / Q000172:deficiency*; D015603:Keratinocytes / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D034741:RNA, Small Interfering