title: Melanoma Regression and Recurrence in Zebrafish.
abstract: Melanoma is the most lethal form of skin cancer with high mortality rates. Most melanoma cases have activating mutations in BRAF (V600E) and the selective inhibitors of BRAF(V600E) have been successfully used in patients. However, after initial tumor regression, the majority of patients develop drug resistance resulting in tumor regrowth. It is therefore important to understand the mechanisms underlying these processes. We have recently described the role of the master melanocyte transcription factor MITF in tumor growth, regression, and recurrence. Here, we describe protocols to study regression and recurrence in vivo, as well as for histology and immunohistochemistry, using a temperature-sensitive zebrafish model of human melanoma.
authors.affiliation: Institute of Genetics and Molecular Medicine, MRC Human Genetics Unit & The University of Edinburgh Cancer Research UK Centre, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, UK.
authors.forename: Sonia
authors.initials: S
authors.lastname: Wojciechowska
authors.affiliation: Institute of Genetics and Molecular Medicine, MRC Human Genetics Unit & The University of Edinburgh Cancer Research UK Centre, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, UK.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Department of Human and Molecular Genetics, Virginia Commonwealth University, School of Medicine Sanger Hall, 11-014, 1101 E. Marshall St. Richmond, VA, 23298-0033, UK.
authors.forename: James A
authors.initials: JA
authors.lastname: Lister
authors.affiliation: Program in Molecular Medicine and Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, 01605, USA.
authors.forename: Craig J
authors.initials: CJ
authors.lastname: Ceol
authors.affiliation: Institute of Genetics and Molecular Medicine, MRC Human Genetics Unit & The University of Edinburgh Cancer Research UK Centre, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, UK. e.patton@igmm.ed.ac.uk.
authors.forename: E Elizabeth
authors.initials: EE
authors.lastname: Patton
mesh_terms: D000818:Animals; D006801:Humans; D007150:Immunohistochemistry; D008545:Melanoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051739:Microphthalmia-Associated Transcription Factor / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics / Q000473:pathology*; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism*
chemical_list: D051739:Microphthalmia-Associated Transcription Factor; D048493:Proto-Oncogene Proteins B-raf
keywords: Melanoma; Mitf; Recurrence; Regression; Zebrafish cancer models