title: PTH/SDF-1α cotherapy induces CD90+CD34- stromal cells migration and promotes tissue regeneration in a rat periodontal defect model.
abstract: Stromal cell-derived factor-1α (SDF-1α) is a key stem cell homing factor that is crucial for recruitment of stem cells to many diseased organs. However, the therapeutic activity of SDF-1α is potentially limited by N-terminal cleavage at position-2 proline by a cell surface protein CD26/dipeptidyl peptidase-IV (DPP-IV). Parathyroid hormone (PTH) is a DPP-IV inhibitor and has been suggested as a promising agent for periodontal tissue repair. The purpose of this study was to explore the effects of a cell-free system comprising SDF-1α and scaffold plus PTH systemic application on periodontal tissue regeneration in vivo. The results showed that PTH/SDF-1α cotherapy improved the quantity of regenerated bone and resulted in better organization of ligament interface. We further investigated the possible mechanisms, and found that PTH/SDF-1α cotherapy enhanced CD90+CD34- stromal cells migration in vivo, increased the number of CXCR4 + cells in periodontal defects, induced early bone osteoclastogenesis and enhanced the expression of runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP) and collagen I (Col I) in newly formed bone tissue. In conclusion, this cell-free tissue engineering system with local administration of SDF-1α and systemic application of PTH could be employed to induce CD90+CD34- stromal cells recruitment and promote periodontal tissue regeneration.
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, China.|Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, China.|Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
authors.forename: Lingqian
authors.initials: L
authors.lastname: Du
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, China.|Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Ge
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001842:Bone and Bones / Q000473:pathology; D002465:Cell Movement; D002474:Cell-Free System; D054377:Chemokine CXCL12 / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D005786:Gene Expression Regulation; D010010:Osteoclasts / Q000166:cytology; D010281:Parathyroid Hormone / Q000378:metabolism*; D010510:Periodontal Diseases / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D017154:Stromal Cells / Q000166:cytology*; D018800:Thy-1 Antigens / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D018952:Antigens, CD34; C575935:CXCL12 protein, rat; D054377:Chemokine CXCL12; D050659:Core Binding Factor Alpha 1 Subunit; D010281:Parathyroid Hormone; C493717:Runx2 protein, rat; D018800:Thy-1 Antigens; D003094:Collagen; D000469:Alkaline Phosphatase