title: Anti-inflammatory effects of 6'-O-acetyl mangiferin from Iris rossii Baker via NF-κb signal blocking in lipopolysaccharide-stimulated RAW 264.7 cells.
abstract: A series of acylated xanthone C-glucosides were identified from the methanolic extract of whole Iris rossii Baker. The major constituent was characterized as 6'-O-acetyl mangiferin (OAM), and complete structure elucidation was carried out using 2D NMR (COSY, HSQC, and HMBC) and LC-IT-TOF-MS analyses. The present study is the first to report the anti-inflammatory effects of 6'-O-acetyl mangiferin from Iris rossii Baker on the lipopolysaccharide (LPS)-induced inflammatory response in RAW264.7 macrophage cells. OAM strongly suppressed protein expression of inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX-2), thereby inhibiting the production of nitric oxide (NO), prostaglandin E2 (PGE2), and cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). Furthermore, OAM inhibited the LPS-induced phosphorylation of ERK, JNK, and p38, which led to the blockade of nuclear factor-kappa B (NF-κB) and inhibitor kappa B (IκB)-α activation. These results suggest that the anti-inflammatory effects of OAM may be attributed to the downregulation of COX-2 and iNOS via the suppression of NF-κB and the MAPK signaling pathway in RAW264.7 macrophages.
authors.affiliation: National Development Institute of Korean Medicine, Jangheung-gun, 59338, Republic of Korea.
authors.forename: Ji-Hun
authors.initials: JH
authors.lastname: Jang
authors.affiliation: National Development Institute of Korean Medicine, Jangheung-gun, 59338, Republic of Korea.
authors.forename: Ki-Ho
authors.initials: KH
authors.lastname: Lee
authors.affiliation: National Development Institute of Korean Medicine, Jangheung-gun, 59338, Republic of Korea.
authors.forename: Ho-Kyung
authors.initials: HK
authors.lastname: Jung
authors.affiliation: National Development Institute of Korean Medicine, Jangheung-gun, 59338, Republic of Korea.
authors.forename: Mi-Ok
authors.initials: MO
authors.lastname: Sim
authors.affiliation: National Development Institute of Korean Medicine, Jangheung-gun, 59338, Republic of Korea.
authors.forename: Tae-Muk
authors.initials: TM
authors.lastname: Kim
authors.affiliation: National Development Institute of Korean Medicine, Jangheung-gun, 59338, Republic of Korea.
authors.forename: Kyeong-Wan
authors.initials: KW
authors.lastname: Woo
authors.affiliation: National Development Institute of Korean Medicine, Jangheung-gun, 59338, Republic of Korea.
authors.forename: Byeong-Kwan
authors.initials: BK
authors.lastname: An
authors.affiliation: National Development Institute of Korean Medicine, Jangheung-gun, 59338, Republic of Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Cho
authors.affiliation: National Development Institute of Korean Medicine, Jangheung-gun, 59338, Republic of Korea. Electronic address: jjhjktm@naver.com.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Cho
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000187:drug effects / Q000378:metabolism; D031323:Iris Plant / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects; D000067996:RAW 264.7 Cells; D015398:Signal Transduction / Q000187:drug effects*; D044004:Xanthones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: C000613755:6'-O-acetylmangiferin; D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D016328:NF-kappa B; D044004:Xanthones; D052247:Nitric Oxide Synthase Type II; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D020928:Mitogen-Activated Protein Kinases
keywords: 6′-O-Acetyl mangiferin; Inflammation; Iris rossii baker; LPS; Raw264.7