title: A novel biomarker for marine environmental pollution of HSP90 from Mytilus coruscus.
abstract: Heat shock protein 90 (HSP90) is a conserved molecular chaperone contributing to cell cycle control, organism development and the proper regulation of cytosolic proteins. The full-length HSP90 cDNA of Mytilus coruscus (McHSP90, KT946644) was 2420bp, including an ORF of 2169bp encoding a polypeptide of 722 amino acids with predicted pI/MW 4.89/83.22kDa. BLASTp analysis and phylogenetic relationship strongly suggested McHSP90 was a member of HSP90 family, and it was highly conserved with other known HSP90, especially in the HSP90 family signatures, ATP/GTP-Binding sites and 'EEVD' motif. The mRNA of McHSP90 in haemolymph was upregulated in all treatments including Vibrio alginolyticus and Vibrio harveyi challenge, metals stresses (copper and cadmium) and 180 CST fuel exposure. All the results implied the expression of McHSP90 could be affected by Vibrio challenge and environmental stress, which might help us gain more insight into the molecular mechanism of HSP against adverse stresses in mollusca.
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China. Electronic address: liuhuihui2004@126.com.
authors.forename: Huihui
authors.initials: H
authors.lastname: Liu
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Wu
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors.forename: Mengshan
authors.initials: M
authors.lastname: Xu
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors.forename: Jianyu
authors.initials: J
authors.lastname: He
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D015415:Biomarkers / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity; D017124:Conserved Sequence; D003300:Copper / Q000633:toxicity; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006458:Hemolymph / Q000378:metabolism; D049878:Mytilus / Q000187:drug effects / Q000502:physiology*; D010802:Phylogeny; D013312:Stress, Physiological; D014733:Vibrio / Q000472:pathogenicity; D014874:Water Pollutants, Chemical / Q000633:toxicity; D014876:Water Pollution / Q000032:analysis*
chemical_list: D015415:Biomarkers; D018076:DNA, Complementary; D018841:HSP90 Heat-Shock Proteins; D014874:Water Pollutants, Chemical; D002104:Cadmium; D003300:Copper
keywords: Environmental stress; Heat shock protein 90 (HSP90); Mytilus coruscus; Vibrio infection