title: Magnetic nanoparticles as a drug delivery system that enhance fungicidal activity of polyene antibiotics.
abstract: This study was designed to assess the antifungal/anti-biofilm and hemolytic properties of two polyene antibiotics, amphotericin B (AMF) and nystatin (NYS), attached to the surface of magnetic nanoparticles (MNP) against clinical isolates of Candida species and human red blood cells, respectively. The developed nanosystems, MNP@AMF and MNP@NYS, displayed stronger fungicidal activity than unbound AMF or NYS. Synergistic activity was observed with a combination of polyenes and MNPs against all tested Candida strains. Nanosystems were more potent than unbound agents when tested against Candida strains in the presence of pus, and as agents able to prevent Candida biofilm formation. The observed inactivation of catalase Cat1 in Candida cells upon treatment with the nanosystems suggests that disruption of the oxidation-reduction balance is a mechanism leading to inhibition of Candida growth. The significant decrease of polyenes lytic activity against host cells after their attachment to MNPs surface indicates improvement in their biocompatibility.
authors.affiliation: Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok, Białystok, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Niemirowicz
authors.affiliation: Holy Cross Oncology Center of Kielce, Kielce, Poland.
authors.forename: Bonita
authors.initials: B
authors.lastname: Durnaś
authors.affiliation: Department of Periodontal and Oral Mucosa Diseases, Medical University of Białystok, Białystok, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Tokajuk
authors.affiliation: Holy Cross Oncology Center of Kielce, Kielce, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Głuszek
authors.affiliation: Institute of Chemistry, University of Białystok, Białystok, Poland.
authors.forename: Agnieszka Z
authors.initials: AZ
authors.lastname: Wilczewska
authors.affiliation: Institute of Chemistry, University of Białystok, Białystok, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Misztalewska
authors.affiliation: Department of Materials and Biomedical Engineering, Białystok University of Technology, Białystok, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Mystkowska
authors.affiliation: Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok, Białystok, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Michalak
authors.affiliation: Regional District Hospital in Kielce, Kielce, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sodo
authors.affiliation: Holy Cross Oncology Center of Kielce, Kielce, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Wątek
authors.affiliation: Department of General Biology, Medical University of Białystok, Białystok, Poland.
authors.forename: Bożena
authors.initials: B
authors.lastname: Kiziewicz
authors.affiliation: Holy Cross Oncology Center of Kielce, Kielce, Poland.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Góźdź
authors.affiliation: Department of Surgery and Surgical Nursing with the Scientific Research Laboratory, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, Poland.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Głuszek
authors.affiliation: Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok, Białystok, Poland; Department of Physiology, Pathophysiology and Microbiology of Infections, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, Poland. Electronic address: buckirobert@gmail.com.
authors.forename: Robert
authors.initials: R
authors.lastname: Bucki
mesh_terms: D000666:Amphotericin B / Q000008:administration & dosage*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D000935:Antifungal Agents; D016503:Drug Delivery Systems; D006801:Humans; D058185:Magnetite Nanoparticles; D008826:Microbial Sensitivity Tests; D011090:Polyenes / Q000008:administration & dosage*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D058185:Magnetite Nanoparticles; D011090:Polyenes; D000666:Amphotericin B
keywords: Biofilm; Candida sp.; Fungal infections; Magnetic nanoparticles; Polyenes antibiotic