title: Investigation of the effect of safranal and crocin pre-treatment on hepatic injury induced by infrarenal aortic occlusion.
abstract: Ischemia-reperfusion (IR) injury of the liver is an unresolved problem that occurs during certain surgical approaches, including hepatic, cardiac and aortic operations. In this study we aimed to investigate whether crocin and safranal had protective effects on liver IR injury induced in an infrarenal aortic clamping (IRAC) model. Male Wistar-Albino rats (n=32) were divided into four groups with 8 animals each as follows: Sham, IR, IR+crocin, and IR+safranal. The infrarenal aorta (IRA) was clamped for 60min for the ischemic period and allowed to reperfuse for 120min. Blood and tissue samples were collected for biochemical, histological and immunohistological analysis. Plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were found to be significantly higher in the IR group than the sham group (respectively; p=0.015, p<0.001). There were significant differences between the IR group and the IR+crocin group or the IR+safranal group in AST levels (respectively; p=0.02, p<0.001). ALT showed a significant decrease in the IR+crocin group compared to the IR group (p<0.05). We also observed histopathological changes among the groups. Bax and Caspase-3 expression in the IR group was remarkably higher than in the other groups. Caspase-3 and Bax expression in the IR+crocin and the IR+safranal groups were significantly lower than in the IR group. Nevertheless, there were no significant differences in BCL2 expression among the groups. IRAC is a cause of IR injury in the liver. This study showed that crocin and safranal have protective effects on IR induced liver injury.
authors.affiliation: Department of General Surgery, Faculty of Medicine, Afyon Kocatepe University, Afyon, 03200, Turkey. Electronic address: tanerozkececi@gmail.com.
authors.forename: Ziya Taner
authors.initials: ZT
authors.lastname: Ozkececi
authors.affiliation: Department of Anatomy, Faculty of Medicine, Afyon Kocatepe University, Afyon, 03200, Turkey.
authors.forename: Yucel
authors.initials: Y
authors.lastname: Gonul
authors.affiliation: Department of Histology and Embryology, Zekai Tahir Burak Women's Health and Research Hospital, Ankara, 06260, Turkey.
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Yuksel
authors.affiliation: Department of Pediatric Surgery, Faculty of Medicine, Afyon Kocatepe University, Afyon, 03200, Turkey.
authors.forename: Afra
authors.initials: A
authors.lastname: Karavelioglu
authors.affiliation: Department of Emergency Medicine, Faculty of Medicine, Afyon Kocatepe University, Afyon, 03200, Turkey.
authors.forename: Kamil
authors.initials: K
authors.lastname: Tunay
authors.affiliation: Department of Anatomy, Faculty of Medicine, Afyon Kocatepe University, Afyon, 03200, Turkey.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Gulsari
authors.affiliation: Department of Anatomy, Faculty of Medicine, Afyon Kocatepe University, Afyon, 03200, Turkey.
authors.forename: Onder
authors.initials: O
authors.lastname: Cartilli
authors.affiliation: Department of Chemistry, Biochemistry Division, Faculty of Science and Arts, Afyon Kocatepe University, Afyonkarahisar 03200, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Hazman
authors.affiliation: Department of General Surgery, Faculty of Medicine, Afyon Kocatepe University, Afyon, 03200, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Bal
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001157:Arterial Occlusive Diseases / Q000097:blood / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D001219:Aspartate Aminotransferases / Q000097:blood; D002338:Carotenoids / Q000627:therapeutic use*; D053148:Caspase 3 / Q000378:metabolism; D053138:Cyclohexenes / Q000494:pharmacology* / Q000627:therapeutic use; D007150:Immunohistochemistry; D007668:Kidney / Q000473:pathology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D008111:Liver Function Tests; D008297:Male; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D013729:Terpenes / Q000494:pharmacology* / Q000627:therapeutic use; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D053138:Cyclohexenes; D013729:Terpenes; D051028:bcl-2-Associated X Protein; D002338:Carotenoids; C087963:safranal; C029036:crocin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D053148:Caspase 3
keywords: Apoptosis; Bax; Blc-2; Caspase-3; Ischemia-reperfusion; Liver injury