title: Analysis and Purification of Mouse Intestinal Dendritic Cell and Macrophage Subsets by Flow Cytometry.
abstract: The unit presents a method for analysis of intestinal dendritic cell (DC) and macrophage subsets by flow cytometry in the single cell suspension prepared from the mouse small and large intestine (Basic Protocol). describes a strategy to enrich the hematopoietic cell fraction in the sample by Percoll gradient centrifugation, and describes preparation of single cell suspensions from specific tissue layers of the small intestine, such as the epithelium, villi mucosa, submucosa, and muscularis externa. Finally, Support Protocol explains how to purify specific intestinal DC and macrophage subsets by flow-cytometry-based cell sorting. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Penn State University College of Medicine, Hershey, Pennsylvania.
authors.forename: Balázs
authors.initials: B
authors.lastname: Koscsó
authors.affiliation: Penn State University College of Medicine, Hershey, Pennsylvania.
authors.forename: Milena
authors.initials: M
authors.lastname: Bogunovic
mesh_terms: D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003713:Dendritic Cells / Q000166:cytology*; D005434:Flow Cytometry; D007413:Intestinal Mucosa / Q000166:cytology*; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D059010:Single-Cell Analysis
keywords: flow cytometry; macrophage subsets; mouse intestinal dendritic cells