title: Simultaneous determination of six mycotoxins in peanut by high-performance liquid chromatography with a fluorescence detector.
abstract: BACKGROUND
  Mycotoxins, which may contaminate peanut and peanut products, are responsible for many diseases to humans. Aflatoxin B1 (AFB1), aflatoxin G1 (AFG1), aflatoxin B2 (AFB2), aflatoxin G2 (AFG2), ochratoxin A (OTA) and zearalenone (ZEN) are considered the most relevant groups of mycotoxins found in food. This work aimed to develop a high-performance liquid chromatography method with a fluorescence detector (HPLC-FLD) combined with dispersive liquid-liquid microextraction (DLLME) method for the simultaneous determination of the six mycotoxins in peanuts. The six mycotoxins were simultaneously determined under their best wavelength by means of changing wavelength.
  
  
  RESULTS
  Under the optimum conditions, the linear ranges were 1-100 ng mL-1for AFB1, AFG1 and OTA, 0.3-30 ng mL-1for AFB2 and AFG2, 5-1000 ng mL-1for ZEN, with the correlation coefficient (R2) of 0.9969-0.9997. Limits of detection (LODs) were 0.10, 0.10, 0.30, 0.03, 0.03 and 1.0 µg kg-1, respectively, and the mean recoveries were in the range of 83.1% to 99.3% with RSD < 10% (n = 6, independent analysis). Thirteen (46%) of these tested samples were contaminated with at least one mycotoxin.
  
  
  CONCLUSIONS
  The proposed method was demonstrated to be simple, highly selective, accurate, reliable, and was successfully applied to simultaneously analyse the six mycotoxins in real peanut samples from China. © 2016 Society of Chemical Industry.
authors.affiliation: School of Public Health, Shandong University, Jinan, 250012, Shandong Province, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Chen
authors.affiliation: School of Public Health, Shandong University, Jinan, 250012, Shandong Province, China.
authors.forename: Chuanlei
authors.initials: C
authors.lastname: Luan
authors.affiliation: School of Public Health, Shandong University, Jinan, 250012, Shandong Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Public Health, Shandong University, Jinan, 250012, Shandong Province, China.
authors.forename: Shue
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Public Health, Shandong University, Jinan, 250012, Shandong Province, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Shao
mesh_terms: D010367:Arachis / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D009183:Mycotoxins / Q000737:chemistry*
chemical_list: D009183:Mycotoxins
keywords: HPLC-FLD; peanut; simultaneous determination; six mycotoxins