title: Attenuation of Isoflurane Preconditioning-Induced Acute Cardioprotection in Hypertensive Hypertrophied Hearts.
abstract: OBJECTIVE
  To evaluate the efficiency of isoflurane-induced anesthetic preconditioning and the role of mitochondrial manganese superoxide dismutase (MnSOD) in hypertensive hypertrophied hearts.
  
  
  METHODS
  A prospective animal investigation.
  
  
  METHODS
  Medical center hospital research laboratory.
  
  
  METHODS
  Male spontaneously hypertensive rats (SHRs) and normotensive control Wistar-Kyoto (WKY) rats.
  
  
  METHODS
  All pentobarbital-anesthetized open-chest rats were subjected to a 45-minute left coronary artery occlusion followed by a 120-minute reperfusion. Before ischemia, both SHR and WKY rats were assigned randomly to receive a 30-minute exposure to 0.9% saline or 1.0 minimum alveolar concentration isoflurane.
  
  
  RESULTS
  The myocardial infarct size, assessed as a percentage of the area at risk, was significantly greater in the hypertrophied SHRs than in the WKY rats (65.3%±8.7% v 51.8%±7.2%, p<0.05). Isoflurane preconditioning appreciably reduced the infarct size in the WKY hearts (30.9%±10.5%, p<0.05) but not in the SHR hearts. MnSOD protein expression and enzymatic activity were increased drastically in response to isoflurane exposure in the hearts of the WKY rats (p<0.05) but not in the SHRs.
  
  
  CONCLUSIONS
  Isoflurane-induced anesthetic preconditioning is attenuated in hypertensive hypertrophied hearts. This impairment may be associated with the loss of MnSOD augmentation during ischemia and reperfusion.
authors.affiliation: Department of Anesthesiology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan; School of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Chen-Hsiu
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Wu
authors.affiliation: Department of Pharmacy and Master Program, Tajen University, Pingtung, Taiwan.
authors.forename: Cheng-Dean
authors.initials: CD
authors.lastname: Shih
authors.affiliation: Department of Anesthesiology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
authors.forename: Wei-Hung
authors.initials: WH
authors.lastname: Lien
authors.affiliation: Department of Anesthesiology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
authors.forename: Shiao-Lin
authors.initials: SL
authors.lastname: Huang
authors.affiliation: Department of Anesthesiology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. Electronic address: kent7892002@msn.com.
authors.forename: Cheng-Cheng
authors.initials: CC
authors.lastname: Huang
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D006973:Hypertension / Q000503:physiopathology*; D017379:Hypertrophy, Left Ventricular / Q000503:physiopathology*; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D007530:Isoflurane / Q000494:pharmacology*; D008297:Male; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
chemical_list: D018685:Anesthetics, Inhalation; D007530:Isoflurane
keywords: hypertension; preconditioning; ventricular hypertrophy; volatile anesthetic