title: Heterogeneity of Leukemic Cells with Basophilic Features: Cytochemical, Ultrastructural and Immunophonotypic Analysis of 8 Cases.
abstract: We have studied leukemic cells, derived from acute nonlymphocytic leukemia with basophilic features and basophilic crisis of chronic myelogenous leukemia (CML), by cytochemical and ultrastructural examination and analysis of surface markers. Cytochemical results varied from case to case, while the ultrastructural appearances of the granules were different from normal granules. The granules had more delicate granular matrices with or without myelinoid figures, whorled or scroll matrix, multivesicular bodies structures, theta granules, and crystalloid structures. Leukemic cells in all cases had myeloid surface markers with some degree of variability. In addition, they were occasionally positive for lymphoid markers, but not for CD10 and IgE receptors. The present results show that leukemic cells with basophilic features are heterogeneous in their morphology, cytochemistry and surface markers.
authors.affiliation: a Second Department of Pathology, Niigata University School of Medicine, Niigata, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fukuda
authors.affiliation: a Second Department of Pathology, Niigata University School of Medicine, Niigata, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kakihara
authors.affiliation: a Second Department of Pathology, Niigata University School of Medicine, Niigata, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohnishi
authors.affiliation: b First Department of Internal Medicine, Niigata University School of Medicine, Niigata, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kishi
authors.affiliation: b First Department of Internal Medicine, Niigata University School of Medicine, Niigata, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Shibata
authors.affiliation: b First Department of Internal Medicine, Niigata University School of Medicine, Niigata, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Narita
authors.affiliation: c Department of Internal Medicine, Nagaoka Red Cross Hospital, Nagaoka, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Kurokawa
authors.affiliation: d Department of Internal Medicine, Niigata Prefectural Central Hospital, Joetsu, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Abe
authors.affiliation: e Department of Oral Pathology, Niigata University School of Dentistry, Niigata, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
keywords: Heterogeneity; cytochemistry ultrastructure; leukemia with basophilic features; surface marker analysis