title: Microbial community structure shifts are associated with temperature, dispersants and nutrients in crude oil-contaminated seawaters.
abstract: This study tracked structure shifts of bacterial compositions before, during and after invading by crude oil to determine the microbial response and explore how temperature, dispersants and nutrients affect the composition of microbial communities or their activities of biodegradation in artificial marine environment. During petroleum hydrocarbons exposed, the composition and functional dynamics of marine microbial communities were altered, favoring bacteria that could utilize this rich carbon source such as the Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes phyla. Low temperature as a dominant factor decreased bacterial richness and catabolic diversity due to abated enzymes activities in correlation with the process of biodegradation. Dispersants exerted no negative consequences on microbial composition, however, bacterial composition by the Chloroflexi, TM6, OP8, Cyanobacteria and Gemmatimonadetes phyla increased. It seemed that more frequent fertilizer application could be equally safe to bacteria and increased significantly the abundance of bacterial strains but Actinobacteria phyla decreased.
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Long
authors.initials: L
authors.lastname: Meng
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao 266100, China. Electronic address: mtbao@ouc.edu.cn.
authors.forename: Mutai
authors.initials: M
authors.lastname: Bao
authors.affiliation: Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology, North China Sea Environmental Monitoring Center, State Oceanic Administration, Qingdao 266033, China.
authors.forename: Peiyan
authors.initials: P
authors.lastname: Sun
mesh_terms: D001419:Bacteria; D001673:Biodegradation, Environmental; D006838:Hydrocarbons / Q000378:metabolism; D010578:Petroleum / Q000378:metabolism*; D012623:Seawater / Q000737:chemistry / Q000382:microbiology; D013696:Temperature; D014871:Water Microbiology
chemical_list: D006838:Hydrocarbons; D010578:Petroleum
keywords: Dispersants; Microbial community; Nutrients; Oil spill; Temperature