title: Deployment of a Reverse Transcription Loop-Mediated Isothermal Amplification Test for Ebola Virus Surveillance in Remote Areas in Guinea.
abstract: To strengthen the laboratory diagnostic capacity for Ebola virus disease (EVD) in the remote areas of Guinea, we deployed a mobile field laboratory and implemented reverse transcription loop-mediated isothermal amplification (RT-LAMP) for postmortem testing. We tested 896 oral swab specimens and 21 serum samples, using both RT-LAMP and reverse transcription-polymerase chain reaction (RT-PCR). Neither test yielded a positive result, and the results from RT-LAMP and RT-PCR were consistent. More than 95% of the samples were tested within 2 days of sample collection. These results highlight the usefulness of the RT-LAMP assay as an EVD diagnostic testing method in the field or remote areas.
authors.affiliation: Institute of Tropical Medicine.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Kurosaki
authors.affiliation: Laboratoire des Fièvres Hémorragiques en Guinée Hôpital National Donka, Service des Maladies Infectieuses et Tropicales Université Gamal Abdel Nasser de Conakry.
authors.forename: N'Faly
authors.initials: N
authors.lastname: Magassouba
authors.affiliation: Laboratoire des Fièvres Hémorragiques en Guinée Hôpital National Donka, Service des Maladies Infectieuses et Tropicales.
authors.forename: Hadja Aïssatou
authors.initials: HA
authors.lastname: Bah
authors.affiliation: Laboratoire des Fièvres Hémorragiques en Guinée Hôpital National Donka, Service des Maladies Infectieuses et Tropicales.
authors.forename: Barré
authors.initials: B
authors.lastname: Soropogui
authors.affiliation: Laboratoire des Fièvres Hémorragiques en Guinée Hôpital National Donka, Service des Maladies Infectieuses et Tropicales.
authors.forename: Amadou
authors.initials: A
authors.lastname: Doré
authors.affiliation: Laboratoire des Fièvres Hémorragiques en Guinée Hôpital National Donka, Service des Maladies Infectieuses et Tropicales.
authors.forename: Fodé
authors.initials: F
authors.lastname: Kourouma
authors.affiliation: Institute of Tropical Medicine.
authors.forename: Mahamoud Sama
authors.initials: MS
authors.lastname: Cherif
authors.affiliation: Ministry of Health, Conakry, Guinea.
authors.forename: Sakoba
authors.initials: S
authors.lastname: Keita
authors.affiliation: Institute of Tropical Medicine Graduate School of Biomedical Sciences, Nagasaki University, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Yasuda
mesh_terms: D029043:Ebolavirus / Q000235:genetics / Q000302:isolation & purification*; D016701:Guinea / Q000453:epidemiology; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D019095:Point-of-Care Systems; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral
keywords: Ebola virus disease; RT-LAMP; field surveillance; mobile laboratory; postmortem testing