title: Characterization of circular RNAs landscape in multiple system atrophy brain.
abstract: Circular RNAs (circRNAs) have been recently identified as a naturally occurring family of widespread and diverse endogenous non-coding RNAs that may regulate gene expression in mammals. They are unusually stable RNA molecules with cell type- or developmental stage-specific expression patterns. However, the role of circRNAs in pathology of complex disease is entirely unknown. Here, we report the specific circular transcriptome in the multiple system atrophy (MSA) brain as determined by RNA sequencing. Five circRNAs, namely IQCK, MAP4K3, EFCAB11, DTNA, and MCTP1, were identified and validated as specifically over-expressed in MSA frontal cortex. The expression levels of linear transcripts were not significantly altered and thus did not follow the pattern of their circular counterparts. Further analysis of expression of five MSA-specific circRNAs revealed their over-expression in the white matter of the MSA cortical tissue. Together, this is the first report describing perturbation of circular transcriptome in α-synucleinopathies.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Bei Jun
authors.initials: BJ
authors.lastname: Chen
authors.affiliation: Deptartment of (Neuro) Pathology, Academic Medical Center and Swammerdam Institute for Life Sciences, Centre for Neuroscience, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: James D
authors.initials: JD
authors.lastname: Mills
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Konii
authors.initials: K
authors.lastname: Takenaka
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Nicola
authors.initials: N
authors.lastname: Bliim
authors.affiliation: Neuroscience Research Australia, Sydney, New South Wales, Australia.|School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Glenda M
authors.initials: GM
authors.lastname: Halliday
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia. m.janitz@unsw.edu.au.
authors.forename: Michael
authors.initials: M
authors.lastname: Janitz
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002540:Cerebral Cortex / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D019578:Multiple System Atrophy / Q000235:genetics* / Q000378:metabolism; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D000079962:RNA, Circular; D017423:Sequence Analysis, RNA; D059467:Transcriptome; D066127:White Matter / Q000378:metabolism
chemical_list: D000079962:RNA, Circular; D012313:RNA
keywords: brain transcriptome; circular RNAs; disease markers; grey matter; multiple system atrophy; white matter