title: Antiproliferative and genotoxic effects of nature identical and artificial synthetic food additives of aroma and flavor.
abstract: This study aimed to analyze the antiproliferative and genotoxic potential of synthetic food flavorings, nature identical passion fruit and artificial vanilla. This assessment used root meristem cells of Allium cepa L., in exposure times of 24 and 48 hours and using doses of 0.2; 0.4 and 0.6 mL. Roots were fixed in Carnoy's solution, hydrolyzed in hydrochloric acid, stained with acetic orcein and analyzed with optical microscope at 400× magnification, 5,000 cells for each treatment. For data analysis, it was used Chi-square test at 5%. Doses of 0.2 mL at ET 48 h; 0.4 and 0.6 mL at ET 24 and 48 h of passion fruit flavor, and the three doses of the vanilla flavor at ET 24 and 48 h significantly reduced the cell division rate in the meristems of roots, proving to be cytotoxic. Doses of 0.2; 0.4 and 0.6 mL of the passion fruit additive, and the three doses of vanilla tested, in the two exposure times, induced mitotic spindle changes and micronuclei formation in the cells of the test organism used, proving to be genotoxic. Therefore, under the studied conditions, flavoring solutions of vanilla and passion fruit, marketed nationally and internationally, significantly altered the functioning of the cell cycle in root meristem cells of A. cepa.
authors.affiliation: Curso de Ciências Biológicas, Departamento de Ciências Biológicas, Universidade Federal do Piauí - UFPI, Campus Senador Helvídio Nunes de Barros, Avenida Cicero Duarte, 950, Bairro Junco, CEP 64600-000, Picos, PI, Brazil.
authors.forename: R D M
authors.initials: RD
authors.lastname: Nunes
authors.affiliation: Curso de Ciências Biológicas, Departamento de Ciências Biológicas, Universidade Federal do Piauí - UFPI, Campus Senador Helvídio Nunes de Barros, Avenida Cicero Duarte, 950, Bairro Junco, CEP 64600-000, Picos, PI, Brazil.
authors.forename: I M S
authors.initials: IM
authors.lastname: Sales
authors.affiliation: Curso de Ciências Biológicas, Departamento de Ciências Biológicas, Universidade Federal do Piauí - UFPI, Campus Senador Helvídio Nunes de Barros, Avenida Cicero Duarte, 950, Bairro Junco, CEP 64600-000, Picos, PI, Brazil.
authors.forename: S I O
authors.initials: SI
authors.lastname: Silva
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal do Piauí - UFPI, Campus Senador Helvídio Nunes de Barros, Avenida Cicero Duarte, 950, Bairro Junco, CEP 64600-000, Picos, PI, Brazil.
authors.forename: J M C
authors.initials: JM
authors.lastname: Sousa
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal do Piauí - UFPI, Campus Senador Helvídio Nunes de Barros, Avenida Cicero Duarte, 950, Bairro Junco, CEP 64600-000, Picos, PI, Brazil.|Programa de Pós-graduação em Genética e Melhoramento - PPGM, Centro de Ciências Agrárias, Campus Ministro Petrônio Portella, Avenida Universitária, Bairro Iningá, lado ímpar, CEP 64049-550, Teresina, PI, Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: Peron
mesh_terms: D002467:Cell Nucleus; D004249:DNA Damage; D005503:Food Additives / Q000633:toxicity*; D018519:Meristem / Q000187:drug effects; D048629:Micronuclei, Chromosome-Defective; D008938:Mitosis; D019697:Onions / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects
chemical_list: D005503:Food Additives