title: MiR-769 promoted cell proliferation in human melanoma by suppressing GSK3B expression.
abstract: MicroRNAs (miRNAs) are short, non-coding RNAs with post-transcriptional regulatory function, playing crucial roles in cancer development and progression of human melanoma. Previous studies have indicated that miR-769 was implicated in diverse biological processes. However, the underlying mechanism of miR-769 in human melanoma has not been intensively investigated. In this present study, we aimed to investigate the role of miR-769 and its target genes in human melanoma. We found that miR-769 expression was strongly increased in human melanoma cells and clinical tissues compared with their corresponding controls. Overexpression of miR-769 promoted cell proliferation in human melanoma cell line A375, whereas miR-769-in reverses the function. Glycogen synthase kinase-3 Beta (GSK3B), a potential target gene of miR-769, and was validated by luciferase assay. Further studies revealed that miR-769 regulated cell proliferation of human melanoma by directly suppressing GSK3B expression and the knockdown of GSK3B expression reversed the effect of miR-769-in on human melanoma cell proliferation. In summary, our data demonstrated that miR-769 might act as a tumor promoter by targeting GSK3B during development of human melanoma.
authors.affiliation: Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China, People's Republic of China; Department of ophthalmology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, People's Republic of China, People's Republic of China.
authors.forename: Hai-Jiang
authors.initials: HJ
authors.lastname: Qiu
authors.affiliation: Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China, People's Republic of China. Electronic address: xiaohelu2011@sina.com.
authors.forename: Xiao-He
authors.initials: XH
authors.lastname: Lu
authors.affiliation: Department of ophthalmology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, People's Republic of China, People's Republic of China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Yang
authors.affiliation: Department of ophthalmology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, People's Republic of China, People's Republic of China.
authors.forename: Chen-Yin
authors.initials: CY
authors.lastname: Weng
authors.affiliation: Department of ophthalmology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, People's Republic of China, People's Republic of China.
authors.forename: E-Keng
authors.initials: EK
authors.lastname: Zhang
authors.affiliation: Department of ophthalmology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, People's Republic of China, People's Republic of China.
authors.forename: Fang-Chao
authors.initials: FC
authors.lastname: Chen
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D000071679:Glycogen Synthase Kinase 3 beta / Q000378:metabolism*; D006801:Humans; D008545:Melanoma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012878:Skin Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C546957:MIRN769 microRNA, human; D035683:MicroRNAs; D000071679:Glycogen Synthase Kinase 3 beta
keywords: Cell proliferation; GSK3B; Human melanoma; miR-769