title: In Vitro Modeling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs).
abstract: The choice of model used to study human respiratory syncytial virus (RSV) infection is extremely important. RSV is a human pathogen that is exquisitely adapted to infection of human hosts. Rodent models, such as mice and cotton rats, are semi-permissive to RSV infection and do not faithfully reproduce hallmarks of RSV disease in humans. Furthermore, immortalized airway-derived cell lines, such as HEp-2, BEAS-2B, and A549 cells, are poorly representative of the complexity of the respiratory epithelium. The development of a well-differentiated primary pediatric airway epithelial cell models (WD-PAECs) allows us to simulate several hallmarks of RSV infection of infant airways. They therefore represent important additions to RSV pathogenesis modeling in human-relevant tissues. The following protocols describe how to culture and differentiate both bronchial and nasal primary pediatric airway epithelial cells and how to use these cultures to study RSV cytopathogenesis.
authors.affiliation: Dentistry and Biomedical Sciences, School of Medicine, Centre for Infection and Immunity, Queen's University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Broadbent
authors.affiliation: Dentistry and Biomedical Sciences, School of Medicine, Centre for Infection and Immunity, Queen's University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK.
authors.forename: Remi
authors.initials: R
authors.lastname: Villenave
authors.affiliation: Dentistry and Biomedical Sciences, School of Medicine, Centre for Infection and Immunity, Queen's University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK.
authors.forename: Hong
authors.initials: H
authors.lastname: Guo-Parke
authors.affiliation: The Royal Belfast Hospital for Sick Children, Belfast Health and Social Services Trust, Belfast, BT12 6BA, UK.
authors.forename: Isobel
authors.initials: I
authors.lastname: Douglas
authors.affiliation: Dentistry and Biomedical Sciences, School of Medicine, Centre for Infection and Immunity, Queen's University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK.|The Royal Belfast Hospital for Sick Children, Belfast Health and Social Services Trust, Belfast, BT12 6BA, UK.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Shields
authors.affiliation: Dentistry and Biomedical Sciences, School of Medicine, Centre for Infection and Immunity, Queen's University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK. u.power@qub.ac.uk.
authors.forename: Ultan F
authors.initials: UF
authors.lastname: Power
mesh_terms: D000072283:A549 Cells; D001980:Bronchi / Q000166:cytology* / Q000821:virology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000821:virology; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D008954:Models, Biological; D009666:Nose / Q000166:cytology* / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000472:pathogenicity*
keywords: Cytopathogenesis; Primary human airway epithelial cells; Respiratory syncytial virus; Well-differentiated human airway epithelial cell culture (WD-PAECs)