title: In vitro toxicity testing of cigarette smoke based on the air-liquid interface exposure: A review.
abstract: Cigarette smoke is a complex aerosol comprising particulate phase and gaseous vapour phase. The air-liquid interface exposure provides a possible technical means to implement whole smoke exposure for the assessment of tobacco products. In this review, the research progress in the in vitro toxicity testing of cigarette smoke based on the air-liquid interface exposure is summarized. The contents presented involve mainly cytotoxicity, genotoxicity, oxidative stress, inflammation, systems toxicology, 3D culture and cigarette smoke dosimetry related to cigarette smoke, as well as the assessment of electronic cigarette aerosol. Prospect of the application of the air-liquid interface exposure method in assessing the biological effects of tobacco smoke is discussed.
authors.affiliation: Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou 450001, China. Electronic address: lixiang79ben@sina.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
mesh_terms: D000336:Aerosols / Q000633:toxicity; D000818:Animals; D018929:Cell Culture Techniques; D066300:Electronic Nicotine Delivery Systems; D006801:Humans; D007249:Inflammation / Q000139:chemically induced; D018384:Oxidative Stress / Q000187:drug effects; D012906:Smoke / Q000009:adverse effects*; D062789:Tobacco Products / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods
chemical_list: D000336:Aerosols; D012906:Smoke
keywords: Air-liquid interface; Cigarette smoke; Whole smoke; in vitro toxicity