title: Royal jelly promotes DAF-16-mediated proteostasis to tolerate β-amyloid toxicity in C. elegans model of Alzheimer's disease.
abstract: Numerous studies have demonstrated that dietary intervention may promote health and help prevent Alzheimer's disease (AD). We recently reported that bee products of royal jelly (RJ) and enzyme-treated royal jelly (eRJ) were potent to promote healthy aging in C. elegans. Here, we examined whether RJ/eRJ consumption may benefit to mitigate the AD symptom in the disease model of C. elegans. Our results showed that RJ/eRJ supplementation significantly delayed the body paralysis in AD worms, suggesting the β-amyloid (Aβ) toxicity attenuation effects of RJ/eRJ. Genetic analyses suggested that RJ/eRJ-mediated alleviation of Aβ toxicity in AD worms required DAF-16, rather than HSF-1 and SKN-1, in an insulin/IGF signaling dependent manner. Moreover, RJ/eRJ modulated the transactivity of DAF-16 and dramatically improved the protein solubility in aged worms. Given protein solubility is a hallmark of healthy proteostasis, our findings demonstrated that RJ/eRJ supplementation improved proteostasis, and this promotion depended on the transactivity of DAF-16. Collectively, the present study not only elucidated the possible anti-AD mechanism of RJ/eRJ, but also provided evidence from a practical point of view to shed light on the extensive correlation of proteostasis and the prevention of neurodegenerative disorders.
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC, USA.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC, USA.|Institute for Engaged Aging, Clemson University, Clemson, SC, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Cao
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC, USA.|Institute for Engaged Aging, Clemson University, Clemson, SC, USA.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Dong
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000494:pharmacology*; D051858:Forkhead Transcription Factors / Q000502:physiology*; D000074702:Proteostasis; D014157:Transcription Factors / Q000502:physiology; D000099219:Royal Jelly
chemical_list: D016229:Amyloid beta-Peptides; D029742:Caenorhabditis elegans Proteins; D004268:DNA-Binding Proteins; D005227:Fatty Acids; D051858:Forkhead Transcription Factors; D014157:Transcription Factors; D000099219:Royal Jelly; C108831:daf-16 protein, C elegans; C474710:heat shock factor-1, C elegans; C073702:skn-1 protein, C elegans
keywords: Alzheimer’s disease; DAF-16; Gerotarget; proteostasis; royal jelly; β-amyloid