title: Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants.
abstract: Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juvenile silver catfish (60-80 g) of both sexes were intraperitoneally vaccinated with BSA (200 µg/fish) alone or mixed to the following adjuvants: Freund's complete adjuvant (FCA), Freund's incomplete adjuvant (FIA), aluminum hydroxide (AlOH), Montanide, four types of cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) and three concentrations of β-glucan, and the immune enhancing property was evaluated by measuring anti-BSA antibodies in blood samples at biweekly intervals. Our results demonstrated that CpGs ODNs and β-glucan were as effective as classical adjuvants (FCA, FIA, AlOH and Montanide) in promoting anti-BSA antibodies and that the kinetics of antibody production induced by all adjuvants used in our study had a similar trend to that observed in other fish species, with a peak at 28 days post-vaccination. These results may be useful for the selection of adjuvants for vaccine formulation intended for silver catfish and for the development of vaccine and vaccination strategies to other fish species.
authors.affiliation: Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo, Passo Fundo, RS, Brasil.
authors.forename: T R
authors.initials: TR
authors.lastname: Pavan
authors.affiliation: Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo, Passo Fundo, RS, Brasil.
authors.forename: J
authors.initials: J
authors.lastname: Di Domenico
authors.affiliation: Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo, Passo Fundo, RS, Brasil.
authors.forename: K S
authors.initials: KS
authors.lastname: Kirsten
authors.affiliation: Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo, Passo Fundo, RS, Brasil.
authors.forename: C O
authors.initials: CO
authors.lastname: Nied
authors.affiliation: Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo, Passo Fundo, RS, Brasil.
authors.forename: R
authors.initials: R
authors.lastname: Frandoloso
authors.affiliation: Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo, Passo Fundo, RS, Brasil.
authors.forename: L C
authors.initials: LC
authors.lastname: Kreutz
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000536:Aluminum Hydroxide / Q000276:immunology; D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D002397:Catfishes / Q000276:immunology*; D002417:Cattle; D005260:Female; D005620:Freund's Adjuvant / Q000276:immunology; D008055:Lipids / Q000276:immunology; D008297:Male; D009838:Oligodeoxyribonucleotides / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D014611:Vaccination / Q000662:veterinary*; D047071:beta-Glucans / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D008055:Lipids; D009838:Oligodeoxyribonucleotides; D047071:beta-Glucans; C114843:incomplete Freund's adjuvant; D012710:Serum Albumin, Bovine; D000536:Aluminum Hydroxide; D005620:Freund's Adjuvant