title: Safety and protective efficacy of a spiC and crp deletion mutant of Salmonella gallinarum as a live attenuated vaccine for fowl typhoid.
abstract: With an aim to develop a safe, immunogenic fowl typhoid (FT) vaccine, the safety and efficacy of 1009ΔspiCΔcrp, a spiC and crp deletion mutant of Salmonella gallinarum, were evaluated in chickens. Three-day-old chickens were intramuscularly immunized with 1009ΔspiCΔcrp (1×10(7)CFU) and boosted 7days later (at 10-days old) with the same dose and via the same route (vaccinated group). The vaccinated group showed no clinical symptoms and no differences in body weight compared to the unvaccinated control group. 1009ΔspiCΔcrp bacteria colonized and persisted in the liver and spleen of vaccinated chickens for >14days, and significant specific humoral and cellular immune responses were induced. Vaccinated chickens were challenged with S. gallinarum strain SG9 at 21days post-immunization (24-day-old chickens), and efficient protection was observed based on the mortality and clinical symptoms, as compared to those in the control group. These results demonstrate that 1009ΔspiCΔcrp can be used as a live attenuated vaccine.
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Junlei
authors.initials: J
authors.lastname: Yin
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Xilong
authors.initials: X
authors.lastname: Kang
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Geng
authors.affiliation: Testing Center, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Maozhi
authors.initials: M
authors.lastname: Hu
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China. Electronic address: zmpan@yzu.edu.cn.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China. Electronic address: jiao@yzu.edu.cn.
authors.forename: Xinan
authors.initials: X
authors.lastname: Jiao
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002645:Chickens; D017353:Gene Deletion; D007111:Immunity, Cellular; D007114:Immunization / Q000662:veterinary; D007273:Injections, Intramuscular; D008099:Liver / Q000276:immunology; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D012475:Salmonella / Q000235:genetics*; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated
keywords: Fowl typhoid; Live attenuated vaccine; Protective efficacy; Safety; Salmonella gallinarum