title: Comparative proteomics reveals the underlying toxicological mechanism of low sperm motility induced by iron ion radiation in mice.
abstract: The toxicological mechanism of low sperm motility induced by iron ion radiation (IIR) was investigated in mice. Reproductive organ indices were measured following whole-body irradiation with a 2Gy iron ion beam. Two-dimensional gel electrophoresis, immunoblotting and immunofluorescence were used to analyze protein expression, and real-time polymerase chain reaction was performed to confirm altered gene expression. Reproductive organ indices and sperm motility were lowest 2 weeks after IIR. Sperm function changes via testis and cauda epididymis function were also determined at this time point. Sixteen differentially expressed proteins were identified in sperm 2 weeks after IIR. Bioinformatics analysis indicated that alpha-enolase (Eno1) may be important in the regulation of glycolysis in sperm, and Eno1 expression was correlated with sperm motility. Eno1 may be a potential marker for low sperm motility induced by IIR, and these results may provide a useful reference for changes in astronaut fertility during long space missions.
authors.affiliation: Department of Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Lanzhou 730000, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Lanzhou 730000, China.
authors.forename: Cuixia
authors.initials: C
authors.lastname: Di
authors.affiliation: Department of Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Lanzhou 730000, China. Electronic address: zhangh@impcas.ac.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D020450:Heavy Ions / Q000009:adverse effects*; D007501:Iron / Q000633:toxicity*; D008297:Male; D051379:Mice; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D040901:Proteomics; D011833:Radiation Injuries, Experimental / Q000378:metabolism*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D014916:Whole-Body Irradiation
chemical_list: D007501:Iron; C457379:Eno1 protein, mouse; D010751:Phosphopyruvate Hydratase
keywords: Motility; Proteomics; Space radiation; Sperm