title: Mouse D1Pas1, a DEAD-box RNA helicase, is required for the completion of first meiotic prophase in male germ cells.
abstract: D1Pas1 is a mouse autosomal DEAD-box RNA helicase expressed predominantly in the testis. To assess its possible function, we generated D1Pas1-deficient mice using embryonic stem cells with a targeted D1Pas1 allele. Deletion of D1Pas1 did not cause noticeable embryonic defects or death, indicating that D1Pas1 is not essential for embryogenesis. Whereas homozygous knockout female mice showed normal reproductive performance, homozygous knockout male mice were completely sterile. The seminiferous epithelium of D1Pas1-deficient males contained no spermatids or spermatozoa because of spermatogenic arrest at the late pachytene stage. Upregulation of retrotransposons such as LINE-1 was not found in D1Pas1-deficient males, unlike males lacking Mvh, another testicular DEAD-box RNA helicase. Meiotic chromosome behavior in developing spermatocytes of D1Pas1-deficient males was indistinguishable from that in wild-type males, at least until synaptonemal complex formation. Thus, mouse D1Pas1 is the first-identified DEAD-box RNA helicase that plays critical roles in the final step of the first meiotic prophase in male germ cells.
authors.affiliation: RIKEN BioResource Center, Tsukuba, Ibaraki, 305-0074, Japan; Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Inoue
authors.affiliation: RIKEN BioResource Center, Tsukuba, Ibaraki, 305-0074, Japan.
authors.forename: Narumi
authors.initials: N
authors.lastname: Ogonuki
authors.affiliation: RIKEN BioResource Center, Tsukuba, Ibaraki, 305-0074, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Hirose
authors.affiliation: RIKEN BioResource Center, Tsukuba, Ibaraki, 305-0074, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hatanaka
authors.affiliation: RIKEN BioResource Center, Tsukuba, Ibaraki, 305-0074, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Matoba
authors.affiliation: Institute for Frontier Medical Sciences, Kyoto University, Kyoto, 606-8507, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Chuma
authors.affiliation: RIKEN BioResource Center, Tsukuba, Ibaraki, 305-0074, Japan.
authors.forename: Kimio
authors.initials: K
authors.lastname: Kobayashi
authors.affiliation: RIKEN BioResource Center, Tsukuba, Ibaraki, 305-0074, Japan.
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Wakana
authors.affiliation: Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Noguchi
authors.affiliation: RIKEN BioResource Center, Tsukuba, Ibaraki, 305-0074, Japan; Graduate School of Life and Environmental Science, University of Tsukuba, Ibaraki, 305-8572, Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Inoue
authors.affiliation: Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. Electronic address: kentaro@m.tohoku.ac.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Tanemura
authors.affiliation: RIKEN BioResource Center, Tsukuba, Ibaraki, 305-0074, Japan; Graduate School of Life and Environmental Science, University of Tsukuba, Ibaraki, 305-8572, Japan; The Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Tokyo, 113-0033, Japan. Electronic address: ogura@rtc.riken.go.jp.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ogura
mesh_terms: D000818:Animals; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism; D005260:Female; D055786:Gene Knockout Techniques; D007248:Infertility, Male / Q000235:genetics / Q000378:metabolism; D008297:Male; D008540:Meiosis; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018626:Retroelements; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism; D013091:Spermatogenesis
chemical_list: D018626:Retroelements; C059787:D1Pas1 protein, mouse; D053487:DEAD-box RNA Helicases
keywords: D1Pas1; Meiosis; RNA helicase; Spermatocyte; Spermatogenesis