title: Stress kinases, endoplasmic reticulum stress, and Alzheimer's disease related markers in peripheral blood mononuclear cells from subjects with increased body weight.
abstract: We aimed to characterize endoplasmic reticulum stress, inflammation, and Alzheimer's disease (AD) related markers in peripheral blood mononuclear cells (PBMCs) from males with varied BMI; and to explore whether high glucose and fatty acids (FFAs) might be critical factors for inducing metabolic alterations in PBMCs under obese condition. Approximately 45 middle-aged men were enrolled with varied BMI. At the protein expression level, compared to the lean, the phosphorylation of AMPK, and p-Akt at serine 473 were significantly reduced from the overweight (OW) and/or obese (OB); while the protein expression of p-JNK, cleaved caspase 3, CHOP and p-eIF2α were elevated from the OW and/or OB. At the mRNA expression level, ER stress markers (i.e. GRP78, CHOP and XBP-1), inflammatory markers (i.e.TLR2, TLR4 and CCR2) and AD markers (i.e. APP, PS1 and PS2) were significantly higher in PBMCs from OB compared to lean. In cultured PBMCs, high glucose and FFAs induced GRP78, CHOP and XBP-1 mRNA, and high glucose also induced APP, PS1 and PS2 mRNA. In conclusion, altered markers including AMPK, ER stress and AD related makers under obese condition could be easily obtained from PBMCs. These markers might provide new mechanistic links between obesity and other metabolic complications including AD.
authors.affiliation: Suzhou Industrial Park center Disease Control &Prevention, 58 Suqian Road, Suzhou, 215123, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Lei
authors.affiliation: Suzhou Industrial Park center Disease Control &Prevention, 58 Suqian Road, Suzhou, 215123, P.R. China.
authors.forename: Lugang
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, 199 Renai Road, Suzhou, 215123, P.R. China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Qin
authors.affiliation: Suzhou Industrial Park center Disease Control &Prevention, 58 Suqian Road, Suzhou, 215123, P.R. China.
authors.forename: Baohui
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, 199 Renai Road, Suzhou, 215123, P.R. China.
authors.forename: Lingmei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, 199 Renai Road, Suzhou, 215123, P.R. China.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Suzhou Industrial Park center Disease Control &Prevention, 58 Suqian Road, Suzhou, 215123, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Suzhou Industrial Park center Disease Control &Prevention, 58 Suqian Road, Suzhou, 215123, P.R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Pang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, 199 Renai Road, Suzhou, 215123, P.R. China.|Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Disease, Soochow University, 199 Renai Road, Suzhou, 215123, P.R. China.
authors.forename: Zhongxiao
authors.initials: Z
authors.lastname: Wan
mesh_terms: D000544:Alzheimer Disease / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D015415:Biomarkers / Q000097:blood*; D015992:Body Mass Index; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress; D006360:Heat-Shock Proteins / Q000097:blood; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications*; D011379:Prognosis; D051742:Transcription Factor CHOP / Q000097:blood; D000071717:X-Box Binding Protein 1 / Q000097:blood
chemical_list: D015415:Biomarkers; C497249:DDIT3 protein, human; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D000071717:X-Box Binding Protein 1; D051742:Transcription Factor CHOP