title: In situ detection of the Zn(2+) release process of ZnO NPs in tumour cells by confocal laser scanning fluorescence microscopy.
abstract: The use of zinc oxide (ZnO) nanoparticles (NPs) for cancer is not yet clear for human clinical applications, which is primarily due to the lack of a better understanding of the action mechanisms and cellular consequences of the direct exposure of cells to these NPs. In this work, the authors have selected zinquin ethyl ester, a Zn(2+)-specific fluorescent molecular probe, to efficiently differentiate ZnO NPs and Zn(2+), and combined with confocal laser scanning microscopy (CLSM) to in situ study the Zn(2+) release process of ZnO NPs in cancer cell system through detecting the change of Zn(2+) level over time. During the experiments, the authors have designed the test group ZnO-2 in addition to assess the influence of a long-term storage on the characteristics of ZnO NPs in aqueous solution, and the Zn(2+) release process of ZnO NPs in cancer cell system. After three-month storage at room temperature, the release process became earlier and faster, which was consistent with previous results of transmission electron microscope, UV-Vis and PL spectra. It is a good detection method that combination of Zn(2+)-specific fluorescent molecular probe and CLSM, which will be helpful for ZnO NPs using in clinical research.
authors.affiliation: Center of Analysis and Measurement, Fudan University, Shanghai, People's Republic of China.
authors.forename: Wenshuang
authors.initials: W
authors.lastname: Song
authors.affiliation: Center of Analysis and Measurement, Fudan University, Shanghai, People's Republic of China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Tang
authors.affiliation: Center of Analysis and Measurement, Fudan University, Shanghai, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Center of Analysis and Measurement, Fudan University, Shanghai, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Departments of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, People's Republic of China.
authors.forename: Jilie
authors.initials: J
authors.lastname: Kong
authors.affiliation: Center of Analysis and Measurement, Fudan University, Shanghai, People's Republic of China. qgren@fudan.edu.cn.
authors.forename: Ren
authors.initials: R
authors.lastname: Qingguang
mesh_terms: D004058:Diffusion; D006367:HeLa Cells; D006801:Humans; D000069416:Intravital Microscopy / Q000379:methods; D053768:Metal Nanoparticles / Q000032:analysis / Q000737:chemistry*; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods; D009374:Neoplasms, Experimental / Q000737:chemistry* / Q000473:pathology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015032:Zinc / Q000032:analysis / Q000737:chemistry*; D015034:Zinc Oxide / Q000032:analysis / Q000737:chemistry*
chemical_list: D015032:Zinc; D015034:Zinc Oxide