title: Oceanisphaera aquimarina sp. nov., Isolated from Oil-Contaminated Sediment of Ocean Coastal Area from South Korea.
abstract: Strain S33(T) was isolated from oil-contaminated sediment of Tae-an coastal region of South Korea. Cells are aerobic, motile, Gram staining-negative, and coccoid shaped. Strain S33(T) grew optimally at the temperature of 25 °C (range of 4-40 °C), pH 6.0 (range of pH 6.0-10.0), and in the presence of 1 % (w/v) NaCl (range of 0-10 %). Ubiquinone-8 was the predominant respiratory quinone. C16:0, summed feature 3 (comprising C16:1ω7c/C16:1ω6c) and C18:1ω7c were the major fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. Strain S33(T) showed the ability to degrade benzene, toluene, and ethylbenzene after 3 days incubation. 16S rRNA gene sequence analysis showed that the strain S33(T) was most closely related to Oceanisphaera sediminis TW92(T) (97.3 %), Oceanisphaera profunda SM1222(T) (97.2 %), and Oceanisphaera ostreae T-w6(T) (97.1 %) and <97 % with other members of the genus Oceanisphaera. The genomic DNA G+C mol% content of strain S33(T) was 51.0 mol%. Based on distinct phenotypic, genotypic, and phylogenetic analysis, strain S33(T) was proposed to represent a novel species in the genus Oceanisphaera as Oceanisphaera aquimarina sp. nov. (= KEMB 1002-058(T) = JCM 30 794(T)).
authors.affiliation: Department of Biological Engineering, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon, 443-760, Republic of Korea.
authors.forename: Seonghyeon
authors.initials: S
authors.lastname: Cho
authors.affiliation: Department of Biological Engineering, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon, 443-760, Republic of Korea. sslee@kyonggi.ac.kr.
authors.forename: Sang-Seob
authors.initials: SS
authors.lastname: Lee
mesh_terms: D044044:Aeromonadaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D010578:Petroleum / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D014877:Water Pollution, Chemical
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010578:Petroleum; D012336:RNA, Ribosomal, 16S