title: Featured Article: Nanoenhanced matrix metalloproteinase-responsive delivery vehicles for disease resolution and imaging.
abstract: The wide array of proteases, including matrix metalloproteinases, produced in response to many pathogenic insults, confers a unique proteolytic signature which is often disease specific and provides a potential therapeutic target for drug delivery. Here we propose the use of collagen-based nanoenhanced matrix metalloproteinase-responsive delivery vehicles that display matrix metalloproteinase-specific degradation in diverse in vitro models of proteolysis. We demonstrate that collagen particles comprised of protease substrates (primarily collagen) can be made of uniform size and loaded efficiently with assorted cargo including fluorescently labeled mesoporous silica, magnetic nanoparticles, proteins and antioxidants. We also demonstrate that pathologic concentrations of proteases produced in situ or in vitro display protease-specific cargo release. Additionally, we show that the collagen-based particles display bright fluorescence when loaded with a fluorophore, and have the potential to be used as vehicles for targeted delivery of drugs or imaging agents to regions of high proteolytic activity.
authors.affiliation: 1 SUNY Polytechnic Institute, Colleges of Nanoscale Science and Engineering, Albany, NY 12203, USA.
authors.forename: Donald A
authors.initials: DA
authors.lastname: McCarthy
authors.affiliation: 1 SUNY Polytechnic Institute, Colleges of Nanoscale Science and Engineering, Albany, NY 12203, USA.
authors.forename: Ahmad A
authors.initials: AA
authors.lastname: Nazem
authors.affiliation: 1 SUNY Polytechnic Institute, Colleges of Nanoscale Science and Engineering, Albany, NY 12203, USA.
authors.forename: James
authors.initials: J
authors.lastname: McNeilan
authors.affiliation: 1 SUNY Polytechnic Institute, Colleges of Nanoscale Science and Engineering, Albany, NY 12203, USA.
authors.forename: Nicole L
authors.initials: NL
authors.lastname: Shakerley
authors.affiliation: 1 SUNY Polytechnic Institute, Colleges of Nanoscale Science and Engineering, Albany, NY 12203, USA.
authors.forename: Ryan R
authors.initials: RR
authors.lastname: Clark
authors.affiliation: 1 SUNY Polytechnic Institute, Colleges of Nanoscale Science and Engineering, Albany, NY 12203, USA.
authors.forename: María D
authors.initials: MD
authors.lastname: Idelchik
authors.affiliation: 2 University at Albany, Albany, NY 12222, USA.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yigit
authors.affiliation: 1 SUNY Polytechnic Institute, Colleges of Nanoscale Science and Engineering, Albany, NY 12203, USA.
authors.forename: J Andrés
authors.initials: JA
authors.lastname: Melendez
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D005347:Fibroblasts / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D066298:In Vitro Techniques; D020782:Matrix Metalloproteinases / Q000008:administration & dosage* / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000627:therapeutic use*; D059748:Proteolysis
chemical_list: D003094:Collagen; D020782:Matrix Metalloproteinases
keywords: Collagen; antioxidants; burn injury; infection; matrix metalloproteinase; nanoparticles; proteases; senescence