title: Use of Moringa oleifera Flower Pod Extract as Natural Preservative and Development of SCAR Marker for Its DNA Based Identification.
abstract: The use of Moringa oleifera as natural food preservative has been evaluated in the present study. In addition, for quality assurance, the study has also been focused on the shelf life of product to authenticate the identification of plant by development of DNA based marker. Among the different extracts prepared from flower pods of Moringa oleifera, methanol and aqueous extract exhibited high antibacterial and antioxidant activity, respectively. The high phenolic contents (53.5 ± 0.169 mg GAE/g) and flavonoid contents (10.9 ± 0.094 mg QE/g) were also recorded in methanol and aqueous extract, respectively. Due to instability of bioactive compounds in aqueous extract, methanol extract is considered as potent natural preservative. The shelf life of methanol extract was observed for two months at 4°C under dark conditions. The developed SCAR primers (MOF217/317/MOR317) specifically amplified a fragment of 317 bp from DNA of Moringa oleifera samples collected from different regions of Punjab province of Pakistan. The methanol extract of Moringa oleifera flower pods has great potential to be used as natural preservative and nutraceutical in food industry.
authors.affiliation: Institute of Biochemistry and Biotechnology, University of the Punjab, Quaid-i-Azam Campus, Lahore 54590, Pakistan.
authors.forename: Iram
authors.initials: I
authors.lastname: Gull
authors.affiliation: Institute of Biochemistry and Biotechnology, University of the Punjab, Quaid-i-Azam Campus, Lahore 54590, Pakistan.
authors.forename: Attia
authors.initials: A
authors.lastname: Javed
authors.affiliation: Institute of Biochemistry and Biotechnology, University of the Punjab, Quaid-i-Azam Campus, Lahore 54590, Pakistan.
authors.forename: Muhammad Shahbaz
authors.initials: MS
authors.lastname: Aslam
authors.affiliation: Department of Biochemistry and Biotechnology, University of Sargodha, Lahore Campus, Lahore 53800, Pakistan.
authors.forename: Roohi
authors.initials: R
authors.lastname: Mushtaq
authors.affiliation: Department of Biochemistry and Biotechnology, University of Sargodha, Lahore Campus, Lahore 53800, Pakistan.
authors.forename: Muhammad Amin
authors.initials: MA
authors.lastname: Athar
mesh_terms: D035264:Flowers / Q000737:chemistry / Q000235:genetics; D005520:Food Preservatives / Q000737:chemistry*; D005819:Genetic Markers; D000432:Methanol / Q000737:chemistry; D029588:Moringa oleifera / Q000737:chemistry / Q000235:genetics; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D005520:Food Preservatives; D005819:Genetic Markers; D010936:Plant Extracts; D000432:Methanol