title: Generalized recovery algorithm for 3D super-resolution microscopy using rotating point spread functions.
abstract: Super-resolution microscopy with phase masks is a promising technique for 3D imaging and tracking. Due to the complexity of the resultant point spread functions, generalized recovery algorithms are still missing. We introduce a 3D super-resolution recovery algorithm that works for a variety of phase masks generating 3D point spread functions. A fast deconvolution process generates initial guesses, which are further refined by least squares fitting. Overfitting is suppressed using a machine learning determined threshold. Preliminary results on experimental data show that our algorithm can be used to super-localize 3D adsorption events within a porous polymer film and is useful for evaluating potential phase masks. Finally, we demonstrate that parallel computation on graphics processing units can reduce the processing time required for 3D recovery. Simulations reveal that, through desktop parallelization, the ultimate limit of real-time processing is possible. Our program is the first open source recovery program for generalized 3D recovery using rotating point spread functions.
authors.affiliation: Department of Chemistry, Rice University, Houston, TX 77251, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Shuang
authors.affiliation: Department of Electrical and Computer Engineering, Rice University, Houston, TX 77251, USA.
authors.forename: Wenxiao
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Rice University, Houston, TX 77251, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Chemistry, Rice University, Houston, TX 77251, USA.
authors.forename: Lawrence J
authors.initials: LJ
authors.lastname: Tauzin
authors.affiliation: Department of Chemistry, Rice University, Houston, TX 77251, USA.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Flatebo
authors.affiliation: Department of Electrical and Computer Engineering, Rice University, Houston, TX 77251, USA.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Rice University, Houston, TX 77251, USA.
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Moringo
authors.affiliation: Department of Chemistry, Rice University, Houston, TX 77251, USA.
authors.forename: Logan D C
authors.initials: LD
authors.lastname: Bishop
authors.affiliation: Department of Electrical and Computer Engineering, Rice University, Houston, TX 77251, USA.
authors.forename: Kevin F
authors.initials: KF
authors.lastname: Kelly
authors.affiliation: Department of Chemistry, Rice University, Houston, TX 77251, USA.|Department of Electrical and Computer Engineering, Rice University, Houston, TX 77251, USA.
authors.forename: Christy F
authors.initials: CF
authors.lastname: Landes