title: Reproductive toxicity of ZishenYutai pill in rats: Perinatal and postnatal development study.
abstract: ZishenYutai pill (ZYP) is one of the most commonly used Chinese patent medicines for threatened miscarriage. Although ZYP is widely used, its toxic effects are rarely assessed. We aimed to investigate whether ZYP had reproductive toxicity during perinatal and postnatal period. Pregnant rats (F0) were continuously exposed to 6, 12 and 24 g/kg body weight/d of ZYP by intragastric administration from gestation day15to post-natal day21. Vehicle and propylthiouracil (PTU, 15 mg/kg) were used as the negative control and positive control, respectively. The mating was done between the treatment (ZYP or PTU) group and negative control group when the F1 pups were born 63-70 days. Body weight, reproductive ability, physical development and neurodevelopment of F0, F1 and F2 pups were observed. The reproductive capacity of F0 and F1 generation decreased significantly after PTU exposure; however, the body weight and reproductive ability of F0, the physical development, weight, feed consumption and reproductive ability of F1, as well as the physical development and body weight of F2 rats were not significantly changed in the ZYP-treated group compared with the negative control group. ZYP exposure had no perinatal toxicity in 3 generations of rats and may be widely used for miscarriage.
authors.affiliation: National Evaluation Centre for the Toxicology of Fertility Regulating Drug, Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, PR China; Key Laboratory of Reproduction Regulation of NPFPC, Shanghai 200032, PR China; Reproductive and Developmental Research Institute of Fudan University, Shanghai, Shanghai 200032, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Guangzhou Baiyunshan Zhongyi Pharmaceutical Co., Ltd, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: National Evaluation Centre for the Toxicology of Fertility Regulating Drug, Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, PR China; Key Laboratory of Reproduction Regulation of NPFPC, Shanghai 200032, PR China; Reproductive and Developmental Research Institute of Fudan University, Shanghai, Shanghai 200032, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Jiang
authors.affiliation: National Evaluation Centre for the Toxicology of Fertility Regulating Drug, Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, PR China; Key Laboratory of Reproduction Regulation of NPFPC, Shanghai 200032, PR China; Reproductive and Developmental Research Institute of Fudan University, Shanghai, Shanghai 200032, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Guangzhou Baiyunshan Zhongyi Pharmaceutical Co., Ltd, PR China.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Huang
authors.affiliation: National Evaluation Centre for the Toxicology of Fertility Regulating Drug, Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, PR China; Key Laboratory of Reproduction Regulation of NPFPC, Shanghai 200032, PR China; Reproductive and Developmental Research Institute of Fudan University, Shanghai, Shanghai 200032, PR China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Yan
authors.affiliation: National Evaluation Centre for the Toxicology of Fertility Regulating Drug, Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, PR China; Key Laboratory of Reproduction Regulation of NPFPC, Shanghai 200032, PR China; Reproductive and Developmental Research Institute of Fudan University, Shanghai, Shanghai 200032, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Guangzhou Baiyunshan Zhongyi Pharmaceutical Co., Ltd, PR China.
authors.forename: Yushuang
authors.initials: Y
authors.lastname: Chai
authors.affiliation: National Evaluation Centre for the Toxicology of Fertility Regulating Drug, Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, PR China; Key Laboratory of Reproduction Regulation of NPFPC, Shanghai 200032, PR China; Reproductive and Developmental Research Institute of Fudan University, Shanghai, Shanghai 200032, PR China.
authors.forename: Liming
authors.initials: L
authors.lastname: Chong
authors.affiliation: National Evaluation Centre for the Toxicology of Fertility Regulating Drug, Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, PR China; Key Laboratory of Reproduction Regulation of NPFPC, Shanghai 200032, PR China; Reproductive and Developmental Research Institute of Fudan University, Shanghai, Shanghai 200032, PR China. Electronic address: sunzy64@163.com.
authors.forename: Zuyue
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000022:Abortion, Spontaneous / Q000188:drug therapy*; D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000633:toxicity*; D004435:Eating / Q000187:drug effects; D005260:Female; D005298:Fertility / Q000187:drug effects; D047109:Fetal Development / Q000187:drug effects; D005865:Gestational Age; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011441:Propylthiouracil / Q000633:toxicity; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects*; D018570:Risk Assessment; D013607:Tablets; D013997:Time Factors; D036401:Toxicity Tests, Chronic; D015430:Weight Gain / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D013607:Tablets; C000615671:Zishen Yutai; D011441:Propylthiouracil
keywords: Chinese patent medicines; Development; Miscarriage; Perinatal toxicity; Rat; Reproductive ability; ZishenYutai pill