title: Effect of short-chain fatty acids on the formation of amylose microparticles by amylosucrase.
abstract: Amylose microparticles can be produced by self-assembly of amylose molecules through an amylosucrase-mediated synthesis. Here we investigated the role of short-chain fatty acids in the formation of amylose microparticles and the fate of these fatty acids at the end of the reaction. The rate of self-assembly and production yields of amylose microparticles were significantly enhanced in the presence of fatty acids. The effect was dependent on the length of the fatty acid carbon tail; butanoic acid (C4) was the most effective, followed by hexanoic acid (C6) and octanoic acid (C8). The amylose microparticles were investigated by carrying out SEM, XRD, Raman, NMR, FT-IR and DSC analysis. The size, morphology and crystal structure of the resulting amylose microparticles were comparable with those of amylose microparticles produced without fatty acids. The results indicated the carboxyl group of the fatty acid to be responsible for promoting the self-assembly of amylose chains to form microparticles. The fatty acids were eventually removed from the microstructure through the tight association of amylose double helices to form the amylose microparticles.
authors.affiliation: Graduate School of Biotechnology & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, Republic of Korea; Korea Food Research Institute, Seongnam, Republic of Korea.
authors.forename: Min-Cheol
authors.initials: MC
authors.lastname: Lim
authors.affiliation: Graduate School of Biotechnology & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, Republic of Korea.
authors.forename: Kyu-Hwan
authors.initials: KH
authors.lastname: Park
authors.affiliation: Graduate School of Biotechnology & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, Republic of Korea.
authors.forename: Jong-Hyun
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Graduate School of Biotechnology & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, Republic of Korea.
authors.forename: Da-Hee
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Graduate School of Biotechnology & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, Republic of Korea.
authors.forename: Carlos Andres Morales
authors.initials: CAM
authors.lastname: Letona
authors.affiliation: Graduate School of Biotechnology & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, Republic of Korea.
authors.forename: Moo-Yeol
authors.initials: MY
authors.lastname: Baik
authors.affiliation: Graduate School of Biotechnology & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, Republic of Korea.
authors.forename: Cheon-Seok
authors.initials: CS
authors.lastname: Park
authors.affiliation: Graduate School of Biotechnology & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, Republic of Korea. Electronic address: youngkim@khu.ac.kr.
authors.forename: Young-Rok
authors.initials: YR
authors.lastname: Kim
mesh_terms: D000688:Amylose / Q000737:chemistry*; D034301:Deinococcus / Q000201:enzymology; D005232:Fatty Acids, Volatile / Q000737:chemistry*; D005964:Glucosyltransferases / Q000378:metabolism*; D008863:Microspheres; D008958:Models, Molecular; D008968:Molecular Conformation
chemical_list: D005232:Fatty Acids, Volatile; D000688:Amylose; D005964:Glucosyltransferases; C019700:amylosucrase
keywords: Amylose microparticle; Amylosucrase; Butanoic acid; Fatty acid; Hexanoic acid; Octanoic acid; Self-assembly