title: Comparison of a new visual isothermal nucleic acid amplification test with PCR and skin snip analysis for diagnosis of onchocerciasis in humans.
abstract: Accurate, simple and affordable diagnostics are needed to detect Onchocerca volvulus infection in humans. A newly developed colorimetric loop-mediated isothermal amplification (LAMP) assay was compared to PCR and skin snip analysis for diagnosis of onchocerciasis. The robustness and simplicity of the assay indicates that it may be a useful field tool for surveillance in endemic countries.
authors.affiliation: New England Biolabs, Ipswich, MA, USA.
authors.forename: Andy
authors.initials: A
authors.lastname: Alhassan
authors.affiliation: Council for Scientific and Industrial Research, Water Research Institute, Accra, Ghana.
authors.forename: Mike Y
authors.initials: MY
authors.lastname: Osei-Atweneboana
authors.affiliation: University of Ghana, Noguchi Memorial Institute for Medical Research, Accra, Ghana.
authors.forename: Kwadwo F
authors.initials: KF
authors.lastname: Kyeremeh
authors.affiliation: New England Biolabs, Ipswich, MA, USA.
authors.forename: Catherine B
authors.initials: CB
authors.lastname: Poole
authors.affiliation: New England Biolabs, Ipswich, MA, USA.
authors.forename: Zhiru
authors.initials: Z
authors.lastname: Li
authors.affiliation: Council for Scientific and Industrial Research, Water Research Institute, Accra, Ghana.
authors.forename: Edward
authors.initials: E
authors.lastname: Tettevi
authors.affiliation: New England Biolabs, Ipswich, MA, USA.
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Tanner
authors.affiliation: New England Biolabs, Ipswich, MA, USA. Electronic address: carlow@neb.com.
authors.forename: Clotilde K S
authors.initials: CK
authors.lastname: Carlow
mesh_terms: D000818:Animals; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D017181:Onchocerca volvulus / Q000235:genetics*; D009855:Onchocerciasis / Q000175:diagnosis* / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012867:Skin / Q000469:parasitology*
keywords: Colorimetric LAMP; Diagnosis; O-150 repeat; Onchocerciasis; Skin snips