title: Classification of Proteus penneri lipopolysaccharides into core region serotypes.
abstract: The frequency of P. penneri isolation from hospital patients, mostly from urine and wounds, keeps on growing, and numerous isolates are multi-drug resistant. P. penneri rods produce lipopolysaccharide (LPS), which may lead to the septic shock. Until now, O-specific polysaccharide has been the best structurally and serologically characterized region of P. penneri LPS. It is worth having an insight into the serological specificity of both poly- and oligosaccharide parts of P. penneri LPS. The P. penneri core region is less structurally diverse than OPS, but still, among other enterobacterial LPS core regions, it is characterized by structural variability. In the present study, the serological reactivity of 25 P. penneri LPS core regions was analyzed by ELISA, passive immunohemolysis and Western blot technique using five polyclonal P. penneri antisera after or without their adsorption with the respective LPSs. The results allowed the assignment of the tested strains to five new core serotypes, which together with published serological studies led to the creation of the first serotyping scheme based on LPS core reactivities of 35 P. penneri and three P. mirabilis strains. Together with the O types scheme, it will facilitate assigning Proteus LPSs of clinical isolates into appropriate O and R serotypes.
authors.affiliation: Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, Banacha 12/16, 90-237, Lodz, Poland. agatapal@biol.uni.lodz.pl.
authors.forename: Agata
authors.initials: A
authors.lastname: Palusiak
mesh_terms: D000818:Animals; D000939:Epitopes / Q000276:immunology; D007106:Immune Sera; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D044095:Proteus penneri / Q000145:classification* / Q000276:immunology* / Q000472:pathogenicity; D011817:Rabbits; D065288:Serogroup; D012703:Serotyping / Q000379:methods; D037521:Virulence Factors
chemical_list: D000939:Epitopes; D007106:Immune Sera; D008070:Lipopolysaccharides; D037521:Virulence Factors
keywords: Classification scheme; Core region; Core serotype; Lipopolysaccharide; Proteus penneri