title: KIF1A mediates axonal transport of BACE1 and identification of independently moving cargoes in living SCG neurons.
abstract: Neurons rely heavily on axonal transport to deliver materials from the sites of synthesis to the axon terminals over distances that can be many centimetres long. KIF1A is the neuron-specific kinesin with the fastest reported anterograde motor activity. Previous studies have shown that KIF1A transports a subset of synaptic proteins, neurofilaments and dense-core vesicles. Using two-colour live imaging, we showed that beta-secretase 1 (BACE1)-mCherry moves together with KIF1A-GFP in both the anterograde and retrograde directions in superior cervical ganglions (SCG) neurons. We confirmed that KIF1A is functionally required for BACE1 transport by using KIF1A siRNA and a KIF1A mutant construct (KIF1A-T312M) to impair its motor activity. We further identified several cargoes that have little or no co-migration with KIF1A-GFP and also move independently from BACE1-mCherry. Together, these findings support a primary role for KIF1A in the anterograde transport of BACE1 and suggest that axonally transported cargoes are sorted into different classes of carrier vesicles in the cell body and are transported by cargo-specific motor proteins through the axon.
authors.affiliation: Department of Signalling Programme, Babraham Institute, Cambridge, UK.
authors.forename: Christy O Y
authors.initials: CO
authors.lastname: Hung
authors.affiliation: Department of Signalling Programme, Babraham Institute, Cambridge, UK. mc469@cam.ac.uk.|John van Geest Centre for Brain Repair, University of Cambridge, Cambridge, UK. mc469@cam.ac.uk.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Coleman
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000378:metabolism*; D001370:Axonal Transport / Q000502:physiology*; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000235:genetics; D016547:Kinesins / Q000235:genetics / Q000502:physiology*; D008164:Luminescent Proteins / Q000235:genetics; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009046:Motor Neurons / Q000378:metabolism / Q000502:physiology*; D009612:Nicotinamide-Nucleotide Adenylyltransferase / Q000378:metabolism; D021381:Protein Transport / Q000502:physiology*; D015398:Signal Transduction; D017783:Superior Cervical Ganglion / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D000097573:Red Fluorescent Protein
chemical_list: D053829:Amyloid Precursor Protein Secretases; D016282:Aspartic Acid Endopeptidases; D049452:Green Fluorescent Proteins; D016547:Kinesins; D008164:Luminescent Proteins; D009612:Nicotinamide-Nucleotide Adenylyltransferase; D000097573:Red Fluorescent Protein; C490576:Kif1a protein, mouse; C579543:Nmnat2 protein, mouse; C509282:Bace1 protein, mouse
keywords: BACE1; KIF1A; axonal transport; live-cell imaging