title: Second-generation probes for biosynthetic intermediate capture: towards a comprehensive profiling of polyketide assembly.
abstract: Malonyl carba(dethia) N-decanoyl cysteamine methyl esters and novel acetoxymethyl esters were utilised as second-generation probes for polyketide intermediate capture. The use of these tools in vivo led to the characterisation of an almost complete set of biosynthetic intermediates from a modular assembly line, providing a first kinetic overview of intermediate processing leading to complex natural product formation.
authors.affiliation: Department of Chemistry, University of Warwick, Library Road, CV4 7AL, UK. M.Tosin@warwick.ac.uk.
authors.forename: Ina
authors.initials: I
authors.lastname: Wilkening
authors.affiliation: Department of Chemistry, University of Warwick, Library Road, CV4 7AL, UK. M.Tosin@warwick.ac.uk and Dipartimento di Scienza ed Alta Tecnologia, Universita' dell'Insubria, Via Valleggio 11, 22100 Como, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Gazzola
authors.affiliation: Department of Chemistry, University of Warwick, Library Road, CV4 7AL, UK. M.Tosin@warwick.ac.uk.
authors.forename: Elena
authors.initials: E
authors.lastname: Riva
authors.affiliation: Department of Chemistry, University of Warwick, Library Road, CV4 7AL, UK. M.Tosin@warwick.ac.uk.
authors.forename: James S
authors.initials: JS
authors.lastname: Parascandolo
authors.affiliation: Department of Chemistry, University of Warwick, Library Road, CV4 7AL, UK. M.Tosin@warwick.ac.uk.
authors.forename: Lijiang
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Chemistry, University of Warwick, Library Road, CV4 7AL, UK. M.Tosin@warwick.ac.uk.
authors.forename: Manuela
authors.initials: M
authors.lastname: Tosin
mesh_terms: D004952:Esters / Q000138:chemical synthesis / Q000737:chemistry*; D007700:Kinetics; D061065:Polyketides / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism
chemical_list: D004952:Esters; D061065:Polyketides