title: Protective effect of carboxytmethylpachymaran on TNF-α-induced damage in Caco-2 cell monolayers.
abstract: This study was carried out to study the protective effect of carboxytmethylpachymaran (CMP) on TNF-α-induced intestinal epithelial barrier dysfunction in Caco-2 cell monolayers and the underlying mechanism. The Caco-2 cell monolayers were pretreated with 50, 100 or 150μg/mL CMP for 72h, and then they were exposed to 100ng/mL tumor necrosis factor alpha (TNF-α) for 72h. The results showed that CMP alleviated the drop of trans-epithelial electrical resistance (TEER) and the increase of phenol red flux induced by TNF-α. CMP also ameliorated TNF-α-induced decrease of mRNA/protein expression and distribution of Occludin and ZO-3 which were chosen as makers of tight junction (TJ). Additionally, the increased protein expressions of MLCK, phosphorylation level of myosin light chain (p-MLC), NF-κB p-P65 and p-IκBα induced by TNF-α were significantly inhibited by CMP. This study demonstrates the protective effect of CMP on TNF-α-induced damage of intestinal epithelial barrier in Caco-2 monolayers and discovers that the suppression of MLCK-p-MLC signaling regulated by NF-κB might be one of the mechanisms underlying the protective effect of CMP.
authors.affiliation: College of the Second Clinical Medicine, Guangzhou University of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, Guangdong Province, China. Electronic address: willdavidsci@163.com.
authors.forename: Jinwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of the Second Clinical Medicine, Guangzhou University of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, Guangdong Province, China. Electronic address: gzyluyue@126.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Lu
authors.affiliation: College of the Second Clinical Medicine, Guangzhou University of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, Guangdong Province, China. Electronic address: andycarrie@163.com.
authors.forename: Jianan
authors.initials: J
authors.lastname: Wei
authors.affiliation: College of the Second Clinical Medicine, Guangzhou University of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, Guangdong Province, China. Electronic address: lily_vvv@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: College of the Second Clinical Medicine, Guangzhou University of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, Guangdong Province, China. Electronic address: linghansci@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Han
mesh_terms: D018938:Caco-2 Cells; D005936:Glucans / Q000493:pharmacokinetics*; D006801:Humans; D007413:Intestinal Mucosa / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D062793:Occludin / Q000096:biosynthesis; D015398:Signal Transduction / Q000187:drug effects*; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D062825:Zonula Occludens Proteins / Q000096:biosynthesis
chemical_list: D005936:Glucans; C568985:OCLN protein, human; D062793:Occludin; C498143:RELA protein, human; C111873:TJP3 protein, human; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D062825:Zonula Occludens Proteins; C568908:carboxymethylpachymaran
keywords: Caco-2 cell; Carboxytmethylpachymaran (CMP); Tumor necrosis factor alpha (TNF-α)