title: Molecular cloning of chicken IL-7 and characterization of its antiviral activity against IBDV in vivo.
abstract: Mammalian interleukin-7 (IL-7) is able to stimulate lymphocyte proliferation and maturation, and reverse immunosuppression. However, whether poultry IL-7 has similar functions remains unclear. Chicken infectious bursal disease virus (IBDV) causes serious immunosuppression in chicken due to virus-induced immune disorder. Whether chicken IL-7 (chIL-7) has the ability to restore the immunity during IBDV-induced immunosuppression is not clear. To test this, we amplified chIL-7 gene by RT-PCR, prepared recombinant chIL-7 using HEK293T cells and treated the chicken with the chIL-7 prior to IBDV infection. Our results indicate that chIL-7 promoted mouse B cell proliferation in vitro, and significantly reduced virus titer in bursal tissue and chicken morbidity of IBDV-infected chicken. Mechanically, chIL-7 induced chicken lymphocyte proliferation and interferon-γ production, but down-regulated TGF-β expression, suggesting that chIL-7 has the ability to reverse IBDV-induced immunosuppression and might be a potential therapeutic agent for prevention and treatment of infectious bursal disease.
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Huo
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
authors.forename: Liyue
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China.
authors.forename: Jianlou
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: Department of Biotechnology, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Cui
authors.affiliation: Department of Biotechnology, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China Lixiujin9824@aliyun.com feizhong2013@126.com.
authors.forename: Xiujin
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China Lixiujin9824@aliyun.com feizhong2013@126.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007243:Infectious bursal disease virus / Q000502:physiology; D015851:Interleukin-7 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000627:therapeutic use*; D051379:Mice; D011201:Poultry Diseases / Q000188:drug therapy* / Q000276:immunology / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use
chemical_list: D030161:Avian Proteins; D018076:DNA, Complementary; D015851:Interleukin-7; D012333:RNA, Messenger; D011994:Recombinant Proteins
keywords: IBDV; chicken interleukin-7; eukaryotic expression; prevention; treatment