title: Disruption of YLR162W in Saccharomyces cerevisiae results in increased tolerance to organic solvents.
abstract: OBJECTIVE
  To identify a novel gene responsible for organic solvent-tolerance by screening a transposon-mediated deletion mutant library based on Saccharomyces cerevisiae L3262.
  
  
  RESULTS
  One strain tolerant of up to 0.5 % (v/v) n-hexane and cyclohexane was isolated. The determination of transposon insertion site identified one gene, YLR162W, and revealed disruption of the ORF of this gene, indicating that organic solvent tolerance can be conferred. Such a tolerant phenotype reverted to the sensitive phenotype on the autologous or overexpression of this gene. This transposon mutant grew faster than the control strain when cultured at 30 °C in YPD medium containing 0.5 % (v/v) n-hexane and cyclohexane respectively.
  
  
  CONCLUSIONS
  Disruption of YLR162W in S. cerevisiae results in increased tolerance to organic solvents.
authors.affiliation: Department of Food Science and Technology, Jungwon University, 85, Munmu-ro, Goesan-eup, Goesan-gun, Chungbuk, 367-805, Republic of Korea. hyun1006@jwu.ac.kr.
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Kim
mesh_terms: D003001:Cloning, Molecular; D003510:Cyclohexanes / Q000633:toxicity*; D015966:Gene Expression Regulation, Fungal; D006586:Hexanes / Q000633:toxicity*; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D009930:Organic Chemicals / Q000633:toxicity; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D012997:Solvents / Q000633:toxicity
chemical_list: D003510:Cyclohexanes; D006586:Hexanes; D009930:Organic Chemicals; D029701:Saccharomyces cerevisiae Proteins; D012997:Solvents; C026385:n-hexane; C506365:Cyclohexane
keywords: Cyclohexane tolerance; Hexane tolerance; Organic solvent tolerance; Saccharomyces cerevisiae; Transposon