title: Formula PSORI-CM01 eliminates psoriasis by inhibiting the expression of keratinocyte cyclin B2.
abstract: BACKGROUND
  Psoriasis is a chronically recurrent inflammatory skin disease, modern medicine could achieve good therapeutic effect, but these treatments led to recurrence of the psoriasis, more severe symptoms due to damaging skin barrier. Traditional Chinese medicine is a useful alternative therapies. The purpose of this study was to explore the mechanism of PSORI-CM01, a Chinese medicine formula for psoriasis therapy, in eliminating psoriasis by studying its effects on inhibiting epidermal hyperplasia.
  
  
  METHODS
  Imiquimod induced psoriasis-form mice model was used to determine the efficacy of PSORICM-01 by assessing the improvement of hyperplasia in epidermal and dermal skin, cyclin B2 expression in skin was detected by immunochemistry. Human keratinocyte cell line HaCaT stimulated by LPS or not was used to research molecular mechanisms of PSORIMCM-01 as in vitro model. The inhibition of proliferation of HaCaT was determined by MTT assay, BrdU assay and real-time cell analysis (RTCA). Cell cycle distribution was detected by flow cytometry. Real-Time PCR and western blot analysis was performed to quantify the mRNA and protein expression levels, respectively. The ability of PSORICM-01 to inhibit proliferation of cyclin B2 overexpressed HaCaT cell were also investigated.
  
  
  RESULTS
  PSORI-CM01 significantly inhibited epidermal hyperplasia in IMQ mice lesion skin, and reduced expression of epidermis cyclin B2. Serum containing PSORI-CM01 dramatically inhibited keratinocyte HaCaT cell proliferation, no matter stimulated by LPS or not. FACS analysis showed ability of PSORICM-01 to arrest cell cycle in the G2/M phase. Additionally, PSORI-CM01 significant downregulated mRNA and protein expression of cyclin B2, and over-expression of cyclin B2 antagonized the anti-proliferative effect of PSORI-CM01 on HaCaT cells.
  
  
  CONCLUSIONS
  PSORI-CM01 inhibits epidermal hyperplasia in imiquimod-induced mouse psoriasis-form model and reduces keratinocyte proliferation in vitro. Our results indicate that PSORI-CM01 may possess therapeutic potential for psoriasis by inhibiting keratinocyte proliferation through downregulation of cyclin B2.
authors.affiliation: Molecular Biology Laboratory, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510120, China.
authors.forename: Jian-An
authors.initials: JA
authors.lastname: Wei
authors.affiliation: Molecular Biology Laboratory, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510120, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Dermatology & Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510120, China. luchuanjian888@vip.sina.com.
authors.forename: Chuan-Jian
authors.initials: CJ
authors.lastname: Lu
authors.affiliation: Pharmaceutics laboratory, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510120, China.
authors.forename: Rui-Zhi
authors.initials: RZ
authors.lastname: Zhao
authors.affiliation: Molecular Biology Laboratory, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510120, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Molecular Biology Laboratory, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510120, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Molecular Biology Laboratory, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510120, China.
authors.forename: Han-Jie
authors.initials: HJ
authors.lastname: Lin
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D056765:Cyclin B2 / Q000378:metabolism*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use*; D006965:Hyperplasia; D015603:Keratinocytes / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D011565:Psoriasis / Q000188:drug therapy*; D012867:Skin / Q000187:drug effects
chemical_list: D056765:Cyclin B2; D004365:Drugs, Chinese Herbal; C000601118:PSORI-CM01
keywords: Chinese medicine formula; Cyclin; Keratinocyte; PSORI-CM01; Psoriasis