title: Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-Temporal Study: Oxidative Stress and Diabetic Salivary Glands.
abstract: Objective. This study evaluated oxidative damage caused to the salivary glands in streptozotocin-induced diabetes (DM). Materials and Methods. Rats were divided into 4 groups: groups 1 and 2, control rats, and groups 3 and 4, DM rats. 8-Hydroxy-2'-deoxyguanosine (8-OHdG), protein carbonyl (PC), 4-hydroxynonenal protein adduct (4-HNE), oxidized and/or MDA-modified LDL-cholesterol (oxy-LDL/MDA), 8-isoprostanes (8-isoP), and oxidative stress index (OSI) were measured at 7 (groups 1 and 3) and 14 (groups 2 and 4) days of experiment. Results. The unstimulated salivary flow in DM rats was reduced in the 2nd week, while the stimulated flow was decreased throughout the duration of the experiment versus control. OSI was elevated in both diabetic glands in the 1st and 2nd week, whereas 8-isoP and 8-OHdG were higher only in the parotid gland in the second week. PC and 4-HNE were increased in the 1st and 2nd week, whereas oxy-LDL/MDA was increased in the 2nd week in the diabetic parotid glands. Conclusions. Diabetes induces oxidative damage of the salivary glands, which seems to be caused by processes taking place in the salivary glands, independently of general oxidative stress. The parotid glands are more vulnerable to oxidative damage in these conditions.
authors.affiliation: Department of Health Care, Higher Vocational School, Noniewicza 10 Street, 16-400 Suwalki, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Knaś
authors.affiliation: Students' Scientific Group "Stomatological Biochemistry", Department of Conservative Dentistry, Medical University of Bialystok, Sklodowskiej M.C. 24a Street, 15-274 Bialystok, Poland.
authors.forename: M
authors.identifier: 0000-0001-5609-3187
authors.initials: M
authors.lastname: Maciejczyk
authors.affiliation: Specialist Dental Practice, I. Daniszewska, Żeromskiego 5 Street, 15-225 Bialystok, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Daniszewska
authors.affiliation: Department of Conservative Dentistry, Medical University Bialystok, Sklodowskiej M.C. 24a Street, 15-274 Bialystok, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Klimiuk
authors.affiliation: County Veterinary Inspection, Zwycięstwa 26B Street, 15-959 Bialystok, Poland.
authors.forename: J
authors.identifier: 0000-0002-7798-8016
authors.initials: J
authors.lastname: Matczuk
authors.affiliation: Department of Conservative Dentistry, Medical University Bialystok, Sklodowskiej M.C. 24a Street, 15-274 Bialystok, Poland.
authors.forename: U
authors.initials: U
authors.lastname: Kołodziej
authors.affiliation: Department of Conservative Dentistry, Medical University Bialystok, Sklodowskiej M.C. 24a Street, 15-274 Bialystok, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Waszkiel
authors.affiliation: Department of Emergency Medicine and Disaster, Medical University of Bialystok, Szpitalna 37 Street, 15-295 Bialystok, Poland.
authors.forename: J R
authors.initials: JR
authors.lastname: Ładny
authors.affiliation: Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, Mickiewicza 2c Street, 15-222 Bialystok, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Żendzian-Piotrowska
authors.affiliation: Department of Conservative Dentistry, Medical University Bialystok, Sklodowskiej M.C. 24a Street, 15-274 Bialystok, Poland.
authors.forename: A
authors.identifier: 0000-0003-4562-0951
authors.initials: A
authors.lastname: Zalewska
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D018384:Oxidative Stress; D050050:Protein Carbonylation; D051381:Rats; D017208:Rats, Wistar; D012463:Saliva / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000447:Aldehydes; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; C075750:8-epi-prostaglandin F2alpha; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D003849:Deoxyguanosine; C027576:4-hydroxy-2-nonenal