title: Boosting dark fermentation with co-cultures of extreme thermophiles for biohythane production from garden waste.
abstract: Proof of principle of biohythane and potential energy production from garden waste (GW) is demonstrated in this study in a two-step process coupling dark fermentation and anaerobic digestion. The synergistic effect of using co-cultures of extreme thermophiles to intensify biohydrogen dark fermentation is demonstrated using xylose, cellobiose and GW. Co-culture of Caldicellulosiruptor saccharolyticus and Thermotoga maritima showed higher hydrogen production yields from xylose (2.7±0.1molmol(-1) total sugar) and cellobiose (4.8±0.3molmol(-1) total sugar) compared to individual cultures. Co-culture of extreme thermophiles C. saccharolyticus and Caldicellulosiruptor bescii increased synergistically the hydrogen production yield from GW (98.3±6.9Lkg(-1) (VS)) compared to individual cultures and co-culture of T. maritima and C. saccharolyticus. The biochemical methane potential of the fermentation end-products was 322±10Lkg(-1) (CODt). Biohythane, a biogas enriched with 15% hydrogen could be obtained from GW, yielding a potential energy generation of 22.2MJkg(-1) (VS).
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Angela A
authors.initials: AA
authors.lastname: Abreu
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Fábio
authors.initials: F
authors.lastname: Tavares
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Maria Madalena
authors.initials: MM
authors.lastname: Alves
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Electronic address: alcina@deb.uminho.pt.
authors.forename: Maria Alcina
authors.initials: MA
authors.lastname: Pereira
mesh_terms: D000693:Anaerobiosis; D056804:Biofuels; D018533:Biomass; D002475:Cellobiose / Q000378:metabolism; D000068016:Eubacteriales / Q000378:metabolism; D000071825:Extremophiles / Q000378:metabolism*; D005285:Fermentation; D000072506:Gardens; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism; D008697:Methane / Q000096:biosynthesis; D020124:Thermotoga maritima / Q000378:metabolism; D014866:Waste Products; D014994:Xylose / Q000378:metabolism
chemical_list: D056804:Biofuels; D014866:Waste Products; D002475:Cellobiose; D006859:Hydrogen; D014994:Xylose; D008697:Methane
keywords: Biohythane; Caldicellulosiruptor bescii; Caldicellulosiruptor saccharolyticus; Co-cultures; Garden waste; Thermotoga maritima