title: Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila.
abstract: High-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. Nevertheless, much remains to be understood about the genetic basis of host plant adaptation. Here, we investigate genome-wide expression in the fly Drosophila buzzatii raised in different conditions. This species uses decaying tissues of cactus of the genus Opuntia as primary rearing substrate and secondarily, the necrotic tissues of the columnar cactus Trichocereus terscheckii. The latter constitutes a harmful host, rich in mescaline and other related phenylethylamine alkaloids. We assessed the transcriptomic responses of larvae reared in Opuntia sulphurea and T. terscheckii, with and without the addition of alkaloids extracted from the latter. Whole-genome expression profiles were massively modulated by the rearing environment, mainly by the presence of T. terscheckii alkaloids. Differentially expressed genes were mainly related to detoxification, oxidation-reduction and stress response; however, we also found genes involved in development and neurobiological processes. In conclusion, our study contributes new data onto the role of transcriptional plasticity in response to alternative rearing environments.
authors.affiliation: IEGEBA-CONICET, UNiversidad de Buenos Aires, FAcultad de Ciencias Exactas y Naturales, Intendente Güiraldes 2160, Ciudad Universitaria (C1428 EHA), CABA, Argentina. dndepanis@ege.fcen.uba.ar.
authors.forename: Diego N
authors.identifier: 0000-0002-3679-9585
authors.initials: DN
authors.lastname: De Panis
authors.affiliation: IEGEBA-CONICET, UNiversidad de Buenos Aires, FAcultad de Ciencias Exactas y Naturales, Intendente Güiraldes 2160, Ciudad Universitaria (C1428 EHA), CABA, Argentina.
authors.forename: Julián
authors.initials: J
authors.lastname: Padró
authors.affiliation: Genomics of Gene Expression Lab, Centro de Investigación Príncipe Felipe, Eduardo Primo Yúfera 3, Valencia, 46012, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Furió-Tarí
authors.affiliation: Genomics of Gene Expression Lab, Centro de Investigación Príncipe Felipe, Eduardo Primo Yúfera 3, Valencia, 46012, Spain.|Department of Applied Statistics, Operations Research and Quality, Universitat Politècnica de València, Camí de Vera, Valencia, 46022, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Tarazona
authors.affiliation: IEGEBA-CONICET, UNiversidad de Buenos Aires, FAcultad de Ciencias Exactas y Naturales, Intendente Güiraldes 2160, Ciudad Universitaria (C1428 EHA), CABA, Argentina.|Laboratorio de Ecosistemas Marinos Fósiles, Instituto de Estudios Andinos Don Pablo Groeber (CONICET-UBA), Intendente Güiraldes 2160, Ciudad Universitaria (C1428 EHA), CABA, Argentina.
authors.forename: Pablo S
authors.initials: PS
authors.lastname: Milla Carmona
authors.affiliation: IEGEBA-CONICET, UNiversidad de Buenos Aires, FAcultad de Ciencias Exactas y Naturales, Intendente Güiraldes 2160, Ciudad Universitaria (C1428 EHA), CABA, Argentina.
authors.forename: Ignacio M
authors.initials: IM
authors.lastname: Soto
authors.affiliation: IEGEBA-CONICET, UNiversidad de Buenos Aires, FAcultad de Ciencias Exactas y Naturales, Intendente Güiraldes 2160, Ciudad Universitaria (C1428 EHA), CABA, Argentina.
authors.forename: Hernán
authors.initials: H
authors.lastname: Dopazo
authors.affiliation: Genomics of Gene Expression Lab, Centro de Investigación Príncipe Felipe, Eduardo Primo Yúfera 3, Valencia, 46012, Spain. aconesa@cipf.es.|Microbiology and Cell Science Department, Institute for Food and Agricultural Sciences, University of Florida at Gainesville, Gainesville, FL, 32603, USA. aconesa@cipf.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Conesa
authors.affiliation: IEGEBA-CONICET, UNiversidad de Buenos Aires, FAcultad de Ciencias Exactas y Naturales, Intendente Güiraldes 2160, Ciudad Universitaria (C1428 EHA), CABA, Argentina. ehasson@ege.fcen.uba.ar.
authors.forename: Esteban
authors.initials: E
authors.lastname: Hasson
mesh_terms: D000222:Adaptation, Physiological; D000470:Alkaloids / Q000737:chemistry*; D000818:Animals; D001118:Argentina; D029421:Cactaceae / Q000737:chemistry*; D003889:Desert Climate; D004330:Drosophila / Q000235:genetics*; D007814:Larva / Q000235:genetics; D059467:Transcriptome
chemical_list: D000470:Alkaloids
keywords: RNA-Seq; alkaloids; environment adaptation; mescaline; plasticity