title: Induction of Biological Apatite Orientation as a Bone Quality Parameter in Bone Regeneration Using Hydroxyapatite/Poly ɛ-Caprolactone Composite Scaffolds.
abstract: Changes in the biological apatite (BAp) c-axis orientation were investigated as a bone quality parameter in bone regeneration using hydroxyapatite/poly ɛ-caprolactone (HA/PCL) composite scaffolds. Three-dimensional (3D) HA/PCL composite scaffolds were fabricated using a layer manufacturing process in three grid sizes (200-, 600-, and 1000 μm) and grafted into the forearm ulna of New Zealand white rabbits. The cross-sectional areas of the bones regenerated from the scaffolds with 600- and 1000-μm grid sizes were significantly larger than those from the scaffold with 200-μm grid sizes, whereas bone mineral density in the regenerated regions did not differ between the three grid sizes. Moreover, the BAp c-axis orientation in the bones regenerated from the scaffolds with grid sizes of 600- and 1000 μm was not significantly different; however, both scaffolds showed enhanced BAp orientation, although the degree of BAp orientation was lower than that in intact bones. In conclusion, HA/PCL composite 3D scaffolds with 600- and 1000-μm grid sizes induced BAp c-axis orientation and showed good bone regeneration behavior in vivo.
authors.affiliation: 1 School of Advanced Materials Engineering, Kookmin University , Seoul, Korea.
authors.forename: Jee-Wook
authors.initials: JW
authors.lastname: Lee
authors.affiliation: 2 Powder and Ceramics Division, Korea Institute of Materials Science , Changwon, Korea.
authors.forename: Hui-Suk
authors.initials: HS
authors.lastname: Yun
authors.affiliation: 3 Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University , Suita, Japan .
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Nakano
mesh_terms: D000818:Animals; D001031:Apatites / Q000737:chemistry*; D015519:Bone Density; D001861:Bone Regeneration / Q000502:physiology*; D001842:Bone and Bones / Q000166:cytology* / Q000502:physiology; D002208:Caproates / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry*; D008297:Male; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001031:Apatites; D002208:Caproates; D007783:Lactones; C121056:caprolactone; D017886:Durapatite