title: Evaluation of the efficacy of a new oral gel containing carvacrol and thymol for home oral care in the management of chronic periodontitis using PCR analysis: a microbiological pilot study.
abstract: The use of chemical devices for domestic oral hygiene in periodontal patients has led to new treatment strategies aiming primarily at a control of infection. Over the last few years, carvacrol and thymol (CT) have been subjected to many scientific and medical studies. The purpose of the present study was to assess the effect of CT on the red complex bacteria using Polymerase Chain Reaction (PCR) for microbiological analysis. Five patients with a diagnosis of chronic periodontitis in the age group >25 years, were selected. None of these patients had received any surgical or non-surgical periodontal therapy and demonstrated radiographic evidence of moderate bone loss. After scaling and root planning, patients received a CT gel to be used at home. Four non-adjacent sites in separate quadrants were selected in each patient for monitoring, based on criteria that the sites localize chronic periodontitis. Microbial analysis (MA) was analyzed at baseline and at day 15. SPSS program was used for statistical purposes and a paired samples correlation was performed at the end of the observation period. Although an absolute reduction was observed among the studied bacteria (i.e. Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Campylobacter rectus and Total bacteria loading) none reach a statistical significant value. The present study demonstrated that CT gel has a small impact on oral biofilm. Additional studies are needed to detect the efficacy of CT gel.
authors.affiliation: Department of Medicine and Surgery, University of Milan-Bicocca, Milan, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Lauritano
authors.affiliation: Department of Chemistry, University of Ferrara, Ferrara, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Pazzi
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Iapichino
authors.affiliation: Department of Medical Sciences, University of Ferrara, Ferrara, Italy.
authors.forename: R M
authors.initials: RM
authors.lastname: Gaudio
authors.affiliation: Ospedale Geriatrico INRCA-IRCCS U. Sestilli, Ancona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Di Muzio
authors.affiliation: Department of Clinical and Experimental Medicine, University of Foggia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Lo Russo
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Pezzetti
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D055113:Chronic Periodontitis / Q000382:microbiology* / Q000628:therapy*; D000081007:Cymenes; D005782:Gels; D006801:Humans; D039821:Monoterpenes / Q000627:therapeutic use*; D009910:Oral Hygiene / Q000379:methods*; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D013943:Thymol / Q000627:therapeutic use*; D014100:Toothpastes / Q000737:chemistry / Q000627:therapeutic use
chemical_list: D000081007:Cymenes; D005782:Gels; D039821:Monoterpenes; D014100:Toothpastes; D013943:Thymol; C073316:carvacrol