title: Pseudomonas kribbensis sp. nov., isolated from garden soils in Daejeon, Korea.
abstract: Two bacterial strains, 46-1 and 46-2T, were isolated from garden soil. These strains were observed to be aerobic, Gram-stain negative, rod-shaped, non-spore-forming, motile and catalase and oxidase positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that the two strains shared 100 % sequence similarity with each other and belong to the genus Pseudomonas in the class Gammaproteobacteria. The concatenated 16S rRNA, gyrB, rpoB and rpoD gene sequences further confirmed that the isolates belong to the Pseudomonas koreensis subgroup (SG), with P. koreensis Ps 9-14T, Pseudomonas moraviensis 1B4Tand Pseudomonas granadensis F-278,770Tas their close relatives (>96 % pairwise similarity). DNA-DNA hybridization with the closely related type strain P. koreensis SG revealed a low level of relatedness (<50 %). A cladogram constructed using whole-cell matrix-assisted laser desorption/ionization time-of-flight (WC-MALDI-TOF) MS analysis showed the isolates formed a completely separate monophyletic group. The isolates were negative for utilization of glycogen, D-psicose, α-keto butyric acid, α-keto valeric acid, succinamic acid and D, L-α-glycerol phosphate. In contrast, all these reactions were positive in P. koreensis JCM 14769Tand P. moraviensis DSM 16007T. The fatty acid C17:0cyclo was detected as one of the major cellular fatty acids (>15 %) in the isolates but it was a minor component (<4 %) in both reference type strains. In contrast, the fatty acid, C12:0was not observed in the isolates but was present in both reference strains. Based on differences such as phylogenetic position, low-level DNA-DNA hybridization, WC-MALDI-TOF MS analysis, fluorescence pigmentation, fatty acid profiles, and substrate utilization, we propose that the isolates 46-1 and 46-2Trepresent a novel species of the genus Pseudomonas, for which the name Pseudomonas kribbensis sp. nov. is proposed; the type strain is 46-2T(=KCTC 32541T= DSM 100278T).
authors.affiliation: Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-gu, Daejeon, 34141, Republic of Korea.
authors.forename: Dong-Ho
authors.initials: DH
authors.lastname: Chang
authors.affiliation: Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-gu, Daejeon, 34141, Republic of Korea.
authors.forename: Moon-Soo
authors.initials: MS
authors.lastname: Rhee
authors.affiliation: Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-gu, Daejeon, 34141, Republic of Korea.
authors.forename: Ji-Sun
authors.initials: JS
authors.lastname: Kim
authors.affiliation: National Institute of Animal Science, 1500 Kongjwipatjwi-ro, Iseo-myeon, Wanju-Gun, Jeollabuk-Do, 55365, Republic of Korea.
authors.forename: Yookyung
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Park
authors.affiliation: Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
authors.forename: Haseong
authors.initials: H
authors.lastname: Kim
authors.affiliation: Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea. sglee@kribb.re.kr.|University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea. sglee@kribb.re.kr.
authors.forename: Seung-Goo
authors.initials: SG
authors.lastname: Lee
authors.affiliation: Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-gu, Daejeon, 34141, Republic of Korea. bckim@kribb.re.kr.|University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea. bckim@kribb.re.kr.
authors.forename: Byoung-Chan
authors.initials: BC
authors.lastname: Kim
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial; D000072506:Gardens; D058889:Molecular Typing; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Garden soil; Polyphasic approaches; Pseudomonadaceae; Pseudomonas kribbensis