title: Local and Acute Disruption of the Yeast Cell Surface.
abstract: In nature, the yeast cell barrier encounters threats ranging from physical impact to abrupt changes in osmolality after rainfall. Genetic materials are protected from these environmental attacks by the rigid cell wall. Laboratory methods for challenging cell wall integrity have made an enormous contribution to the study of the yeast cell surface, but most have targeted whole-cell populations in place of single-cell analysis. This protocol describes pulse-laser-based acute disruption of the yeast cell surface, which enables the observation of single-cell response to submicron-scale damage.
authors.affiliation: Department of Cell Biology, Graduate School of Medical Sciences, Nagoya City University, 1, Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi Prefecture 467-8601, Japan;
authors.forename: Keiko
authors.initials: K
authors.lastname: Kono
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture 277-8562, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Okada
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture 277-8562, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ohya
mesh_terms: D002473:Cell Wall / Q000528:radiation effects*; D007834:Lasers; D012441:Saccharomyces cerevisiae / Q000528:radiation effects*; D059010:Single-Cell Analysis / Q000379:methods*; D013499:Surface Properties / Q000528:radiation effects*