title: A Precise Chemical Strategy To Alter the Receptor Specificity of the Adeno-Associated Virus.
abstract: The ability to target the adeno-associated virus (AAV) to specific types of cells, by altering the cell-surface receptor it binds, is desirable to generate safe and efficient therapeutic vectors. Chemical attachment of receptor-targeting agents onto the AAV capsid holds potential to alter its tropism, but is limited by the lack of site specificity of available conjugation strategies. The development of an AAV production platform is reported that enables incorporation of unnatural amino acids (UAAs) into specific sites on the virus capsid. Incorporation of an azido-UAA enabled site-specific attachment of a cyclic-RGD peptide onto the capsid, retargeting the virus to the αv β3 integrin receptors, which are overexpressed in tumor vasculature. Retargeting ability was site-dependent, underscoring the importance of achieving site-selective capsid modification. This work provides a general chemical approach to introduce various receptor binding agents onto the AAV capsid with site selectivity to generate optimized vectors with engineered infectivity.
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Kelemen
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.
authors.forename: Raja
authors.initials: R
authors.lastname: Mukherjee
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.
authors.forename: Xiaofu
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.
authors.forename: Sarah B
authors.initials: SB
authors.lastname: Erickson
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.
authors.forename: Yunan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA. abhishek.chatterjee@bc.edu.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Chatterjee
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D002213:Capsid / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D000229:Dependovirus / Q000737:chemistry* / Q000502:physiology; D016503:Drug Delivery Systems; D018014:Gene Transfer Techniques; D057809:HEK293 Cells; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D008958:Models, Molecular; D009369:Neoplasms / Q000098:blood supply / Q000378:metabolism; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000378:metabolism; D053586:Virus Internalization
chemical_list: D000596:Amino Acids; D039302:Integrin alphaVbeta3; D010456:Peptides, Cyclic; C507169:cyclic arginine-glycine-aspartic acid peptide
keywords: adeno-associated virus; chemical conjugation; genetic code expansion; viral entry