title: Case report: on the use of the HID-Ion AmpliSeq™ Ancestry Panel in a real forensic case.
abstract: In the absence of any other conclusive forensic evidence, DNA profiling is the method of choice for body identification. This study focuses on the case of a carbonized corpse whose complete autosomal short tandem repeat (STR) profile could not lead to direct identification by the investigators. To assist in the progress of investigation, we endeavoured to determine the biogeographical origin and eye colour of the deceased individual. Along with Y chromosome and mitochondrial DNA analyses, we applied a next-generation sequencing (NGS) approach to the study of ancestry informative markers (AIMs) using the HID-Ion AmpliSeq™ Ancestry Panel launched by Thermo Fisher Scientific. This work gave us the opportunity to test this new technology in a real forensic case. Although this study highlights the benefits of such a combined approach, as it markedly improves the specificity of the biogeographical profile, it also underlines the need for the accurate characterization of a larger collection of reference populations and the necessity of caution in data interpretation.
authors.affiliation: Institute of Legal Medicine, Université de Strasbourg, 11 rue Humann, 67085, Strasbourg, France. hollard@unistra.fr.
authors.forename: C
authors.initials: C
authors.lastname: Hollard
authors.affiliation: Institute of Legal Medicine, Université de Strasbourg, 11 rue Humann, 67085, Strasbourg, France.|AMIS Laboratory, CNRS UMR 5288, Université de Toulouse, 37 Allées Jules Guesde, Toulouse, 31073, France.|Institut National de la Transfusion Sanguine (INTS), 6 rue Alexandre Cabanel, 75015, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Keyser
authors.affiliation: Institute of Legal Medicine, Université de Strasbourg, 11 rue Humann, 67085, Strasbourg, France.|Institut National de la Transfusion Sanguine (INTS), 6 rue Alexandre Cabanel, 75015, Paris, France.
authors.forename: T
authors.initials: T
authors.lastname: Delabarde
authors.affiliation: Institute of Legal Medicine, Université de Strasbourg, 11 rue Humann, 67085, Strasbourg, France.
authors.forename: A
authors.initials: A
authors.lastname: Gonzalez
authors.affiliation: AMIS Laboratory, CNRS UMR 5288, Université de Toulouse, 37 Allées Jules Guesde, Toulouse, 31073, France.|Institut National de la Transfusion Sanguine (INTS), 6 rue Alexandre Cabanel, 75015, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Vilela Lamego
authors.affiliation: AMIS Laboratory, CNRS UMR 5288, Université de Toulouse, 37 Allées Jules Guesde, Toulouse, 31073, France.
authors.forename: V
authors.initials: V
authors.lastname: Zvénigorosky
authors.affiliation: AMIS Laboratory, CNRS UMR 5288, Université de Toulouse, 37 Allées Jules Guesde, Toulouse, 31073, France.|Institut National de la Transfusion Sanguine (INTS), 6 rue Alexandre Cabanel, 75015, Paris, France.|Institute of Legal Medicine, 2 voie Mazas, 75012, Paris, France.|Université Paris Descartes, 12 rue de l'Ecole de Médecine, 75006, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Ludes
mesh_terms: D000071298:Body Remains; D002056:Burns; D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting; D004272:DNA, Mitochondrial / Q000235:genetics*; D030561:Databases, Nucleic Acid; D005127:Eye Color / Q000235:genetics; D005819:Genetic Markers; D005828:Genetics, Population; D005838:Genotype; D006239:Haplotypes; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers
keywords: Ancestry; Degraded DNA; Next-generation sequencing; Unidentified body