title: Insulin in combination with selenium inhibits HG/Pal-induced cardiomyocyte apoptosis by Cbl-b regulating p38MAPK/CBP/Ku70 pathway.
abstract: In this study, we investigated whether insulin and selenium in combination (In/Se) suppresses cardiomyocyte apoptosis and whether this protection is mediated by Cbl-b regulating p38MAPK/CBP/Ku70 pathway.
  
  
  
  Firstly, H9c2 cardiomyocytes were treatment with high glucose (25 mmol/L) and palmitate (600 μmol/L) (HG/Pal). Next, H9c2 cardiomyocytes were treatment with HG/Pal+In/Se (10 nmol/L Insulin in combination with 10 nmol/L selenium). Finally, cells were treated with siRNA against Cbl-b, followed by HG/Pal and HG/Pal+In/Se treatment. Then, Cell apoptosis was observed by flow cytometry (FCM). The levels of Cbl-b, p-p38MAPK, CBP and Bax were examined by Western blotting. The acetylated Ku70 was detected by immunoprecipitation.
  
  
  
  Insulin and selenium in combination reduced cell apoptosis, up-regulated Cbl-b expression, down-regulated p38MAPK, CBP and acetylated Ku70 expressions and prevented Bax translocation, whereas Cbl-b knockdown strongly suppressed In/Se-induced these effects in HG/Pal-treated cardiomyocytes.
  
  
  
  Insulin and selenium synergistically suppressed cardiomyocyte apoptosis by Cbl-b regulating p38MAPK/CBP/Ku70 pathway.
authors.affiliation: Department of Pharmacology, Xi'an Medical University, Xi'an, Shaanxi, PR China. e-mail: xtj1118@163.com.
authors.forename: T-J
authors.initials: TJ
authors.lastname: Xu
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: P
authors.initials: P
authors.lastname: Li
authors.forename: X L
authors.initials: XL
authors.lastname: Xu
authors.forename: J R
authors.initials: JR
authors.lastname: Zeng
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D004357:Drug Synergism; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D007328:Insulin; D048051:p38 Mitogen-Activated Protein Kinases; D012643:Selenium