title: Ionofore antibiotic polynactin produced by Streptomyces sp. 156A isolated from Lake Baikal.
abstract: The potential antibacterial activity of secondary metabolites produced by Streptomyces sp. 156A isolated from Lake Baikal was investigated. The selective liquid-liquid extraction method was applied to obtain a mixture of nactins (polynactin) produced by the strain. The polynactin consisted of nonactin (3%), monactin (18%), dinactin (36%), trinactin (31%) and tetranactin (12%). The compounds were identified by MS/MS,1H and13C NMR methods. The loss of neutral 184 and 198 Da fragments from a sodiated molecular ion, [M + Na]+, of nactins was observed in the MS/MS spectrum. The polynactin was shown to possess the antibiotic activity against Gram-positive strains including opportunistic strains and strains isolated from various ecosystems of Lake Baikal.
authors.affiliation: a Siberian Branch, Limnological Institute , Russian Academy of Sciences , Irkutsk , Russia.
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Shishlyannikova
authors.affiliation: a Siberian Branch, Limnological Institute , Russian Academy of Sciences , Irkutsk , Russia.
authors.forename: Anton V
authors.initials: AV
authors.lastname: Kuzmin
authors.affiliation: a Siberian Branch, Limnological Institute , Russian Academy of Sciences , Irkutsk , Russia.
authors.forename: Galina A
authors.initials: GA
authors.lastname: Fedorova
authors.affiliation: a Siberian Branch, Limnological Institute , Russian Academy of Sciences , Irkutsk , Russia.
authors.forename: Sergey M
authors.initials: SM
authors.lastname: Shishlyannikov
authors.affiliation: a Siberian Branch, Limnological Institute , Russian Academy of Sciences , Irkutsk , Russia.
authors.forename: Irina A
authors.initials: IA
authors.lastname: Lipko
authors.affiliation: a Siberian Branch, Limnological Institute , Russian Academy of Sciences , Irkutsk , Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Sukhanova
authors.affiliation: a Siberian Branch, Limnological Institute , Russian Academy of Sciences , Irkutsk , Russia.
authors.forename: Natalia L
authors.initials: NL
authors.lastname: Belkova
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D060106:Lakes / Q000382:microbiology; D059625:Liquid-Liquid Extraction; D018942:Macrolides / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D012426:Russia; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013302:Streptomyces / Q000737:chemistry* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; C100287:monactin; C009584:trinactin; C016520:dinactin; C050476:nonactin
keywords: Lake Baikal; Secondary metabolites; Streptomyces; nactins