title: IMMUNOLOCALIZATION OF INHIBIN/ACTIVIN SUBUNITS AND STEROIDOGENIC ENZYMES IN THE TESTES OF AN ADULT AFRICAN ELEPHANT (LOXODONTA AFRICANA).
abstract: In this case report, the authors investigated immunolocalization of inhibin α and inhibin/activin βA and βB subunits, as well as steroidogenic enzymes, in the testes of an African elephant. Testes were collected from a reproductively active male African elephant (24 yr old) at autopsy. Histologically, all types of spermatogenic cells including mature-phase spermatozoa were found in the seminiferous tubules. Positive immunostaining for inhibin α and inhibin/activin βA and βB subunits was observed in Sertoli and Leydig cells. In addition, P450scc, 3βHSD, P450c17, and P450arom were also detected in the cytoplasm of Leydig cells. These results suggested that Leydig cells of adult African elephant testes have the ability to synthesize progestin, androgen, and estrogen, whereas both Sertoli and Leydig cells appear as a major source of inhibin secretion in the male African elephant.
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Li
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Weng
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Kawakami
authors.forename: Eriko
authors.initials: E
authors.lastname: Saito
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Saroch
authors.initials: S
authors.lastname: Kaewmanee
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nagaoka
authors.forename: Gen
authors.initials: G
authors.lastname: Watanabe
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Taya
mesh_terms: D028341:Activins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D004606:Elephants / Q000502:physiology*; D005786:Gene Expression Regulation; D007265:Inhibins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D021122:Protein Subunits; D013737:Testis / Q000201:enzymology* / Q000378:metabolism
chemical_list: D021122:Protein Subunits; C094377:inhibin-alpha subunit; D028341:Activins; D007265:Inhibins
keywords: African elephant; immunohistochmestry; inhibin; steroidogenic enzyme; testis