title: Enhancement of UV-induced nucleotide excision repair activity upon forskolin treatment is cell growth-dependent.
abstract: Forskolin (FSK), an adenylyl cyclase activator, has recently been shown to enhance nucleotide excision repair (NER) upon UV exposure. However, our study revealed that this effect was detected in human skin epithelial ARPE19 cells only in growing cells, but not in non-cycling cells. When the cells were grown at low density (70% confluence), FSK was capable of stimulating cAMP responsive element binding (CREB) phosphorylation, a marker for FSK-stimulated PKA activation, and resulted in a significant increase of NER activity compared to control treatment. However, cells grown under 100% confluent conditions showed neither FSK-induced CREB phosphorylation nor the resulting NER enhancement. These findings indicate that cellular growth is critical for FSK-induced NER enhancement and suggest that cellular growth conditions should be considered as a variable while evaluating a reagent's pharmacotherapeutic efficacy. [BMB Reports 2016; 49(10): 566-571].
authors.affiliation: Department of Biological Science, Dong-A University, Busan 49315, Korea.
authors.forename: Jeong-Min
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Department of Biological Science, Dong-A University, Busan 49315, Korea.
authors.forename: Jeong-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Biological Science, Dong-A University, Busan 49315, Korea.
authors.forename: Tae-Hong
authors.initials: TH
authors.lastname: Kang
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D005576:Colforsin / Q000494:pharmacology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D015151:Immunoblotting; D010766:Phosphorylation / Q000187:drug effects; D014466:Ultraviolet Rays
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D005576:Colforsin; D017868:Cyclic AMP-Dependent Protein Kinases