title: Sensitizing DNA Towards Low-Energy Electrons with 2-Fluoroadenine.
abstract: 2-Fluoroadenine ((2F) A) is a therapeutic agent, which is suggested for application in cancer radiotherapy. The molecular mechanism of DNA radiation damage can be ascribed to a significant extent to the action of low-energy (<20 eV) electrons (LEEs), which damage DNA by dissociative electron attachment. LEE induced reactions in (2F) A are characterized both isolated in the gas phase and in the condensed phase when it is incorporated into DNA. Information about negative ion resonances and anion-mediated fragmentation reactions is combined with an absolute quantification of DNA strand breaks in (2F) A-containing oligonucleotides upon irradiation with LEEs. The incorporation of (2F) A into DNA results in an enhanced strand breakage. The strand-break cross sections are clearly energy dependent, whereas the strand-break enhancements by (2F) A at 5.5, 10, and 15 eV are very similar. Thus, (2F) A can be considered an effective radiosensitizer operative at a wide range of electron energies.
authors.affiliation: Institute of Chemistry-Physical Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam, Germany.
authors.forename: Jenny
authors.initials: J
authors.lastname: Rackwitz
authors.affiliation: Faculty of Sciences, Siedlce University, 3 Maja 54, 08-110, Siedlce, Poland.
authors.forename: Janina
authors.initials: J
authors.lastname: Kopyra
authors.affiliation: Department of Chemistry, University of Gdańsk, 80-952, Gdańsk, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Dąbkowska
authors.affiliation: Institute of Chemistry-Physical Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam, Germany.
authors.forename: Kenny
authors.initials: K
authors.lastname: Ebel
authors.affiliation: Institute of Physics Belgrade, University of Belgrade, Pregrevica 118, 11080, Belgrade, Serbia.
authors.forename: MiloŠ Lj
authors.initials: MLj
authors.lastname: Ranković
authors.affiliation: Institute of Physics Belgrade, University of Belgrade, Pregrevica 118, 11080, Belgrade, Serbia.|SOLEIL, l'Orme des Merisiers, St. Aubin, BP48, 91192, Gif sur Yvette Cedex, France.
authors.forename: Aleksandar R
authors.initials: AR
authors.lastname: Milosavljević
authors.affiliation: Institute of Chemistry-Physical Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam, Germany. ilko.bald@uni-potsdam.de.|Department 1-Analytical Chemistry and Reference Materials, BAM Federal Institute for Materials Research and Testing, Richard-Willstätter Str. 11, 12489, Berlin, Germany. ilko.bald@uni-potsdam.de.
authors.forename: Ilko
authors.initials: I
authors.lastname: Bald
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004583:Electrons; D011789:Quantum Theory
chemical_list: C038208:2-fluoroadenine; D004247:DNA; D000225:Adenine
keywords: DNA origami; DNA radiation damage; ab initio calculations; dissociative electron attachment; fludarabine