title: YMO1 suppresses invasion and metastasis by inhibiting RhoC signaling and predicts favorable prognosis in hepatocellular carcinoma.
abstract: Previous studies have shown that 4.1 proteins, which are deregulated in many cancers, contribute to cell adhesion and motility. Yurt/Mosaic eyes-like 1 (YMO1) is a member of 4.1 protein family but it is unclear whether YMO1 plays a role in tumor invasion. This study aimed to investigate the effects of YMO1 on hepatocellular carcinoma (HCC) and attempted to elucidate the underlying molecular mechanisms. YMO1 expression in HCC tissues and its correlation with clinicopathological features and postoperative prognosis was analyzed. The results showed that YMO1 was down-regulated in the highly metastatic HCC cell line and in human tumor tissues. Underexpression of YMO1 indicated poor prognosis of HCC patients. Restoration of YMO1 expression caused a significant decrease in cell migration and invasiveness in vitro. In vivo study showed that YMO1 reduced liver tumor invasion and metastasis in xenograft mice. YMO1 directly inhibited RhoC activation. YMO1 expression in HCC was regulated by PAX5. Analysis of YMO1 expression levels in human HCC patients revealed a significant correlation of YMO1 expression with PAX5 and RhoC. Our findings revealed that YMO1 predicts favorable prognosis and the data suggest that YMO1 suppresses tumor invasion and metastasis by inhibiting RhoC activity.
authors.affiliation: Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Rui-Min
authors.initials: RM
authors.lastname: Chang
authors.affiliation: Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Pei
authors.affiliation: Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Fang
authors.affiliation: Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Jiang-Feng
authors.initials: JF
authors.lastname: Xu
authors.affiliation: Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Abdominal Surgical Oncology, Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
authors.forename: Chao-Hui
authors.initials: CH
authors.lastname: Zuo
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhou
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Geng-Qiu
authors.initials: GQ
authors.lastname: Luo
authors.affiliation: Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China.|Department of Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Lian-Yue
authors.initials: LY
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008297:Male; D008565:Membrane Proteins / Q000502:physiology*; D051379:Mice; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D051757:PAX5 Transcription Factor / Q000502:physiology; D011379:Prognosis; D015398:Signal Transduction / Q000502:physiology*; D054460:rho-Associated Kinases / Q000502:physiology; D000075024:rhoC GTP-Binding Protein / Q000037:antagonists & inhibitors* / Q000502:physiology
chemical_list: C525177:EPB41L5 protein, human; D008565:Membrane Proteins; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases; C066690:RHOC protein, human; D000075024:rhoC GTP-Binding Protein
keywords: PAX5; RhoC; YMO1; hepatocellular carcinoma; metastasis