title: Omics Studies of the Murine Intestinal Ecosystem Exposed to Subchronic and Mild Social Defeat Stress.
abstract: The microbiota-gut-brain axis plays an important role in the development of stress-induced mental disorders. We previously established the subchronic and mild social defeat stress (sCSDS) model, a murine experimental model of depression, and investigated the metabolomic profiles of plasma and liver. Here we used omics approaches to identify stress-induced changes in the gastrointestinal tract. Mice exposed to sCSDS for 10 days showed the following changes: (1) elevation of cholic acid and reduction of 5-aminovaleric acid among cecal metabolites; (2) downregulation of genes involved in the immune response in the terminal ileum; (3) a shift in the diversity of the microbiota in cecal contents and feces; and (4) fluctuations in the concentrations of cecal metabolites produced by gut microbiota reflected in plasma and hepatic metabolites. Operational taxonomic units within the family Lachnospiraceae showed an inverse correlation with certain metabolites. The social interaction score correlated with cecal metabolites, IgA, and cecal and fecal microbiota, suggesting that sCSDS suppressed the ileal immune response, altering the balance of microbiota, which together with host cells and host enzymes resulted in a pattern of accumulated metabolites in the intestinal ecosystem distinct from that of control mice.
authors.affiliation: Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO) , Tsukuba, Ibaraki 305-0901, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Aoki-Yoshida
authors.affiliation: Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO) , Tsukuba, Ibaraki 305-0901, Japan.
authors.forename: Reiji
authors.initials: R
authors.lastname: Aoki
authors.affiliation: Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO) , Tsukuba, Ibaraki 305-0901, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Moriya
authors.affiliation: College of Agriculture, Ibaraki University , Ami, Ibaraki 300-0393, Japan.|Ibaraki University Cooperation between Agriculture and Medical Science (IUCAM) , Ami, Ibaraki 300-0393, Japan.
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Goto
authors.affiliation: College of Agriculture, Ibaraki University , Ami, Ibaraki 300-0393, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Kubota
authors.affiliation: College of Agriculture, Ibaraki University , Ami, Ibaraki 300-0393, Japan.|Ibaraki University Cooperation between Agriculture and Medical Science (IUCAM) , Ami, Ibaraki 300-0393, Japan.|United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology , Fuchu-city, Tokyo 183-8509, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Toyoda
authors.affiliation: Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO) , Tsukuba, Ibaraki 305-0901, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Takayama
authors.affiliation: Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO) , Tsukuba, Ibaraki 305-0901, Japan.
authors.forename: Chise
authors.initials: C
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism* / Q000382:microbiology; D007082:Ileum / Q000276:immunology / Q000378:metabolism; D055432:Metabolomics; D051379:Mice; D064307:Microbiota / Q000276:immunology*; D040901:Proteomics; D013315:Stress, Psychological / Q000276:immunology / Q000378:metabolism*
keywords: cecum; cholic acid; depression; ileum; microbiota-gut-brain axis; social defeat stress