title: Synthesis of Polydopamine Functionalized Reduced Graphene Oxide-Palladium Nanocomposite for Laccase Based Biosensor.
abstract: Graphene based 2D nanomaterials have attracted increasing attention in biosensing application due to the outstanding physicochemical properties of graphene. In this work, palladium nanoparticles (Pd) loaded reduced graphene oxide (rGO) hybrid (rGO-Pd) was synthesized through a facile method. Laccase (Lac) was immobilized on rGO-Pd by utilizing the self-polymerization of dopamine, which generated polydopamine (PDA). The PDA-Lac-rGO-Pd nanocomposites were further modified on electrode surface to construct novel biosensing platform. The obtained electrochemical biosensor was applied in the detection of catechol, achieving excellent analytic results. Under the optimum condition, this biosensor possessed a linear range from 0.1 µM to 263 µM for catechol detection, the sensitivity reached 18.4 µA mM(-1), and the detection limit was as low as 0.03 µM. In addition, the biosensor also showed good repeatability, reproducibility, anti-interference, and stability. Moreover, the novel Lac based biosensor was successfully used in the trace detection of catechol existing in real water environment.
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Da-Wei
authors.identifier: 0000-0002-5460-442X
authors.initials: DW
authors.lastname: Li
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Luo
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Lv
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, Wuxi 214122, China.
authors.forename: Ke-Yu
authors.identifier: 0000-0002-6395-9628
authors.initials: KY
authors.lastname: Lu
authors.affiliation: Key Laboratory of Textile Fabric, Anhui Polytechnic University, Wuhu, Anhui 241000, China.
authors.forename: An-Fang
authors.initials: AF
authors.lastname: Wei
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Qu-Fu
authors.identifier: 0000-0003-2143-9939
authors.initials: QF
authors.lastname: Wei