title: Assessment of different pre-treatment methods for the removal of limonene in citrus waste and their effect on methane potential and methane production rate.
abstract: The objective of this study was to assess the limonene removal efficiency of three pre-treatment methods when applied to citrus waste and to evaluate their effects on the biochemical methane potential and the methane production rate using batch anaerobic tests. The methods tested were based on removal (biological pretreatment by fungi) or recovery (steam distillation and ethanol extraction) of limonene. All the treatments decreased the concentration of limonene in orange peel, with average efficiencies of 22%, 44% and 100% for the biological treatment, steam distillation and ethanol extraction, respectively. By-products from limonene biodegradation by fungi exhibited an inhibitory effect also, not making interesting the biological pretreatment. The methane potential and production rate of the treated orange peel increased significantly after applying the recovery strategies, which separated and recovered simultaneously other inhibitory components of the citrus essential oil. Apart from the high recovery efficiency of the ethanol extraction process, it presented a favourable energy balance.
authors.affiliation: AINIA Technology Centre, Parque Tecnológico Valencia, Valencia, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Ruiz
authors.affiliation: AINIA Technology Centre, Parque Tecnológico Valencia, Valencia, Spain.
authors.forename: Amparo
authors.initials: A
authors.lastname: de Benito
authors.affiliation: AINIA Technology Centre, Parque Tecnológico Valencia, Valencia, Spain.
authors.forename: José Daniel
authors.initials: JD
authors.lastname: Rivera
authors.affiliation: Department of Agrifood Engineering and Biotechnology, Universitat Politècnica de Catalunya, Barcelona, Spain xavier.flotats@upc.edu.
authors.forename: Xavier
authors.initials: X
authors.lastname: Flotats
mesh_terms: D000818:Animals; D056804:Biofuels; D001709:Biotechnology / Q000379:methods; D002417:Cattle; D002849:Chromatography, Gas; D032084:Citrus sinensis / Q000737:chemistry* / Q000378:metabolism; D053138:Cyclohexenes / Q000737:chemistry*; D057168:Distillation; D000431:Ethanol / Q000737:chemistry; D005260:Female; D005638:Fruit / Q000737:chemistry; D000077222:Limonene; D008372:Manure; D008697:Methane / Q000096:biosynthesis*; D013227:Steam; D013729:Terpenes / Q000737:chemistry*; D018505:Waste Management / Q000379:methods*
chemical_list: D056804:Biofuels; D053138:Cyclohexenes; D008372:Manure; D013227:Steam; D013729:Terpenes; D000431:Ethanol; D000077222:Limonene; D008697:Methane
keywords: Citrus waste; anaerobic digestion; ethanol extraction; fungal pretreatment; inhibition; limonene; steam distillation