title: Characterization of Immune System Cell Subsets in Fixed Tissues from Alpine Chamois (Rupicapra rupicapra).
abstract: Immune system cell subsets in lymph nodes and spleen from alpine chamois (Rupicapra rupicapra subspecies rupicapra) living in the Italian Alps were characterized immunohistochemically. Seven primary antibodies (against human CD3, CD79αcy, CD68, or ovine CD4, CD8, CD21 and γδ T-cell receptor [TCR] epitopes) were tested on tissues fixed either in formalin or in zinc salts (ZS) and cross-reactivity with chamois immune cell epitopes was shown. ZS fixation allowed wider identification of immune cells, without the need for antigen retrieval. CD4(+) and CD21(+) cells were labelled only in ZS-fixed tissues. Reagents specific for human CD3, CD79 and CD68 antigens successfully detected chamois immune cells, both in ZS-fixed and formalin-fixed tissues. The reactivity and distribution of immune cells in lymph nodes and spleen were similar to those described in other domestic and wild ruminants. Results from this study may allow future investigation of the immune response and pathogenesis of diseases in the chamois.
authors.affiliation: Department of Veterinary Sciences, Pisa University, Viale delle Piagge, Pisa, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Salvadori
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Near Edinburgh, UK.
authors.forename: J
authors.initials: J
authors.lastname: Finlayson
authors.affiliation: Department of Veterinary Sciences and Public Health, Milan University, Via Festa del Perdono, Milano, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Trogu
authors.affiliation: Department of Veterinary Sciences and Public Health, Milan University, Via Festa del Perdono, Milano, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Formenti
authors.affiliation: Department of Veterinary Sciences and Public Health, Milan University, Via Festa del Perdono, Milano, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Lanfranchi
authors.affiliation: Istituto Zooprofilattico Sperimentale delle Venezie, Sezione di Belluno, Via M. Cappellari, Belluno, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Citterio
authors.affiliation: Biomathematics and Statistics Scotland, JCMB, The King's Buildings, Peter Guthrie Tait Road, Edinburgh, UK.
authors.forename: J
authors.initials: J
authors.lastname: Palarea-Albaladejo
authors.affiliation: Department of Veterinary Sciences, Pisa University, Viale delle Piagge, Pisa, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Poli
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Near Edinburgh, UK. Electronic address: Francesca.Chianini@Moredun.ac.uk.
authors.forename: F
authors.initials: F
authors.lastname: Chianini
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D005260:Female; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000276:immunology*; D008297:Male; D048608:Rupicapra / Q000276:immunology*; D013154:Spleen / Q000276:immunology*; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D015703:Antigens, CD
keywords: alpine chamois; immune system cells; immunohistochemistry; zinc salts fixation