title: Immune-inflammation gene signatures in endometriosis patients.
abstract: OBJECTIVE
  To determine if the molecular profiles of endometriotic lesions contain informative measures of inflammation and immune dysfunction that may contribute to better understanding of the interplay between immune dysfunction and inflammation and their contribution to endometriosis pathogenesis.
  
  
  METHODS
  Immune and inflammation transcriptomic analysis with the use of the Nanostring nCounter GX Human Immunology V2 platform (579 human immune and inflammation-related genes and 15 housekeeping genes).
  
  
  METHODS
  Academic university and teaching hospital.
  
  
  METHODS
  None.
  
  
  METHODS
  Stage III-IV endometriosis patients with infertility (n = 8) and fertile disease-free control women undergoing tubal ligation (n = 8). Menstrual stage was matched to secretory phase in all participants.
  
  
  METHODS
  Immune and inflammation transcriptomics quantification from ectopic endometriotic lesions and matched eutopic endometrium from patients. Endometria of fertile women served as control subjects.
  
  
  RESULTS
  Our results displayed endometriotic lesions as molecularly distinct entities compared with eutopic endometrium and endometrium of control samples; 396 out of 579 screened immune and inflammation-related genes were significantly different in ectopic tissues compared with control endometrium. Most importantly, eutopic endometrium of the patients displayed a unique molecular profile compared with the control endometrium (91/579 genes were significantly different), particularly of genes involved in regulation of cell apoptosis and decidualization.
  
  
  CONCLUSIONS
  We characterize differential expression of immune-inflammation genes in endometriosis patients, and show molecular distinction of eutopic endometrium of patients compared with control fertile women.
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Ahn
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
authors.forename: Kasra
authors.initials: K
authors.lastname: Khalaj
authors.affiliation: Department of Obstetrics and Gynecology, University of North Carolina, Chapel Hill, North Carolina.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Young
authors.affiliation: Department of Obstetrics and Gynecology, Greenville Health Systems, Greenville, South Carolina.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Lessey
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
authors.forename: Madhuri
authors.initials: M
authors.lastname: Koti
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada. Electronic address: tayadec@queensu.ca.
authors.forename: Chandrakant
authors.initials: C
authors.lastname: Tayade
mesh_terms: D017209:Apoptosis / Q000235:genetics; D016022:Case-Control Studies; D002448:Cell Adhesion / Q000235:genetics; D002633:Chemotaxis / Q000235:genetics; D016000:Cluster Analysis; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D004715:Endometriosis / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology*; D004717:Endometrium / Q000276:immunology* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D005819:Genetic Markers; D006784:Hospitals, Teaching; D006801:Humans; D007249:Inflammation / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology*; D008213:Lymphocyte Activation / Q000235:genetics; D018121:Receptors, Cytokine / Q000235:genetics / Q000276:immunology; D059467:Transcriptome
chemical_list: D016207:Cytokines; D005819:Genetic Markers; D018121:Receptors, Cytokine
keywords: Angiogenesis; endometriosis; immune genes; infertility; inflammation