title: Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens.
abstract: Because little is known about the impact of chelated (Fe-Gly, Fe-Gly+F) and inorganic (FeSO4, FeSO4+F) iron products on immune response parameters in broiler chickens, the objective of the study was to determine the effects of inorganic and organic forms of iron on selected parameters of the cell-mediated immune response in broiler chickens by assessing the percentage of CD3(+)CD4(+), CD3(+)CD8(+), CD25(+), and MHC Class II lymphocytes, as well as the CD4(+)/CD8(+) ratio and IL-2 concentration in the peripheral blood. The experiments were conducted using 50day-old Ross 308 roosters. The test material was peripheral blood. Flow cytometry was used to determine selected cell-mediated immune response parameters. The results obtained indicate that the use of iron chelates in the diet of broiler chickens may stimulate cellular defense mechanisms. As a result of the experiment an increase was observed in the percentage of Th1, mainly T CD4(+) and T CD8(+). It was also noted that application of chelated iron can increase production of T CD8(+) cytotoxic cells and IL-2, which promotes the body's natural response to developing inflammation. There were no changes in T CD4(+), T CD8(+), T CD25(+) or MHC II lymphocyte subpopulations in the chickens following application of the inorganic form of iron.
authors.affiliation: Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland. Electronic address: lukasz.jarosz@up.lublin.pl.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Jarosz
authors.affiliation: Faculty of Biology and Animal Breeding, Institute of Animal Nutrition and Bromatology, Department of Animal Nutrition, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kwiecień
authors.affiliation: Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Marek
authors.affiliation: Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Grądzki
authors.affiliation: Faculty of Biology and Animal Breeding, Institute of Animal Nutrition and Bromatology, Department of Bromatology and Nutrition Physiology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Winiarska-Mieczan
authors.affiliation: Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Kalinowski
authors.affiliation: Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Laskowska
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002645:Chickens / Q000276:immunology; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D005296:Ferrous Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D005998:Glycine / Q000008:administration & dosage / Q000494:pharmacology*; D007111:Immunity, Cellular / Q000187:drug effects* / Q000276:immunology; D007376:Interleukin-2 / Q000097:blood; D008214:Lymphocytes / Q000145:classification / Q000502:physiology; D008297:Male; D013431:Sulfates
chemical_list: D005296:Ferrous Compounds; D007376:Interleukin-2; D013431:Sulfates; C020748:ferrous sulfate; D005998:Glycine
keywords: Chicken; Flow cytometry; Il-2; Iron chelate