title: Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish.
abstract: We present a protocol to combine behavioral recording and imaging using 2-photon laser-scanning microscopy in head-fixed larval zebrafish that express a genetically encoded calcium indicator. The steps involve restraining the larva in agarose, setting up optics that allow projection of a visual stimulus and infrared illumination to monitor behavior, and analysis of the neuronal and behavioral data.
authors.affiliation: Champalimaud Neuroscience Programme, Champalimaud Centre for the Unknown, Avenida Brasília, Doca de Pedrouços, Lisbon, 1400-038, Portugal.
authors.forename: Michael B
authors.initials: MB
authors.lastname: Orger
authors.affiliation: Max Planck Institute of Neurobiology, Sensorimotor Control Research Group, Am Klopferspitz 18, Martinsried, 82152, Germany. rportugues@neuro.mpg.de.
authors.forename: Ruben
authors.initials: R
authors.lastname: Portugues
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001921:Brain / Q000378:metabolism / Q000502:physiology*; D007814:Larva / Q000502:physiology*; D008853:Microscopy; D015027:Zebrafish
keywords: 2-Photon microscopy; Behavior; Whole-brain imaging; Zebrafish