title: Streptomyces castaneus sp. nov., a novel actinomycete isolated from the rhizosphere of Peucedanum praeruptorum Dunn.
abstract: During an investigation of microbial diversity in medicinal herbs, a novel actinomycete, strain NEAU-QHHV11Twas isolated from the rhizosphere of Peucedanum praeruptorum Dunn collected from Xianglu Mountain in Heilongjiang Province, northeast China and characterized using a polyphasic approach. The organism was found to have typical characteristics of the genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequence also indicated that strain NEAU-QHHV11Tbelongs to the genus Streptomyces and was most closely related to Streptomyces graminilatus NBRC 108882T(98.7 % sequence similarity) and Streptomyces turgidiscabies NBRC 16080T(98.7 % sequence similarity). The results of DNA-DNA hybridization and some phenotypic characteristics indicated that strain NEAU-QHHV11Tcould be distinguished from its close phylogenetic relatives. Thus, strain NEAU-QHHV11Trepresents a novel species of the genus Streptomyces, for which the name Streptomyces castaneus sp. nov. is proposed. The type strain is NEAU-QHHV11T(=CGMCC 4.7235T= DSM 100520T).
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, People's Republic of China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, People's Republic of China.
authors.forename: Zhilei
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Bai
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yan
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, People's Republic of China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, People's Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Lu
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, People's Republic of China.
authors.forename: Chongxi
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, People's Republic of China. wangneau2013@163.com.
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, People's Republic of China. xiangwensheng@neau.edu.cn.|State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China. xiangwensheng@neau.edu.cn.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Xiang
mesh_terms: D019661:Apiaceae / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene; Peucedanum praeruptorum Dunn; Polyphasic taxonomy; Streptomyces castaneus sp. nov.