title: Genome-Wide Identification and Characterization of Carboxypeptidase Genes in Silkworm (Bombyx mori).
abstract: The silkworm (Bombyx mori) is an economically-important insect that can secrete silk. Carboxypeptidases have been found in various metazoan species and play important roles in physiological and biochemical reactions. Here, we analyzed the silkworm genome database and characterized 48 carboxypeptidases, including 34 metal carboxypeptidases (BmMCP1-BmMCP34) and 14 serine carboxypeptidases (BmSCP1-BmSCP14), to better understand their diverse functions. Compared to other insects, our results indicated that carboxypeptidases from silkworm have more family members. These silkworm carboxypeptidases could be divided into four families: Peptidase_M2 carboxypeptidases, Peptidase_M14 carboxypeptidases, Peptidase_S10 carboxypeptidases and Peptidase_S28 carboxypeptidases. Microarray analysis showed that the carboxypeptidases had distinct expression patterns, whereas quantitative real-time PCR demonstrated that the expression level of 13 carboxypeptidases significantly decreased after starvation and restored after re-feeding. Overall, our study provides new insights into the functional and evolutionary features of silkworm carboxypeptidases.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. yejunhong0129@outlook.com.
authors.forename: Junhong
authors.initials: J
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. yili89716@gmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. lhw888718@163.com.
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. lijifu0103@163.com.
authors.forename: Jifu
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. dong-zhaoming@163.com.
authors.forename: Zhaoming
authors.initials: Z
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. xiaqy@swu.edu.cn.
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. zhaop@swu.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
mesh_terms: D000818:Animals; D012831:Bombyx / Q000201:enzymology / Q000235:genetics*; D002268:Carboxypeptidases / Q000145:classification* / Q000235:genetics*; D020869:Gene Expression Profiling; D049750:Genome, Insect / Q000235:genetics*; D005810:Multigene Family; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D002268:Carboxypeptidases
keywords: Bombyx mori; carboxypeptidase family; evolutionary analysis; expression pattern; starvation