title: Penicillium subrubescens is a promising alternative for Aspergillus niger in enzymatic plant biomass saccharification.
abstract: In industrial applications, efficient mixtures of polysaccharide-degrading enzymes are needed to convert plant biomass into fermentable sugars. Most of the commercially produced lignocellulolytic enzymes are from a limited number of filamentous fungi, such as Trichoderma and Aspergillus species. In contrast, the plant biomass-degrading capacity of Penicillia has been less explored. We performed growth profiling of several Penicillia on diverse plant biomass-related substrates demonstrating the capacity particularly of Penicillium subrubescens to degrade crude lignocellulose feedstock, as well as polysaccharides, and metabolise their monomeric components. We focussed on the lignocellulolytic potential of P. subrubescens FBCC1632, which produced a variable set of (hemi-)cellulolytic activities on plant biomass substrates with activity levels comparable to those of Aspergillus niger. The good ability of the extracellular enzyme mixtures produced by P. subrubescens to saccharify complex plant biomasses, wheat bran and sugar beet pulp, indicated a high potential for this strain as a producer of industrial enzyme cocktails.
authors.affiliation: Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, Viikki Biocenter 1, University of Helsinki, Finland.
authors.forename: Miia R
authors.initials: MR
authors.lastname: Mäkelä
authors.affiliation: Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, Viikki Biocenter 1, University of Helsinki, Finland.
authors.forename: Sadegh
authors.initials: S
authors.lastname: Mansouri
authors.affiliation: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, Utrecht, The Netherlands.
authors.forename: Ad
authors.initials: A
authors.lastname: Wiebenga
authors.affiliation: Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, Viikki Biocenter 1, University of Helsinki, Finland.
authors.forename: Johanna
authors.initials: J
authors.lastname: Rytioja
authors.affiliation: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, Utrecht, The Netherlands. Electronic address: r.devries@cbs.knaw.nl.
authors.forename: Ronald P
authors.initials: RP
authors.lastname: de Vries
authors.affiliation: Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, Viikki Biocenter 1, University of Helsinki, Finland.
authors.forename: Kristiina S
authors.initials: KS
authors.lastname: Hildén
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D018533:Biomass; D001709:Biotechnology; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D007218:Industrial Microbiology; D008031:Lignin / Q000378:metabolism; D010407:Penicillium / Q000201:enzymology* / Q000254:growth & development; D011134:Polysaccharides / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D011134:Polysaccharides; C036909:lignocellulose; D008031:Lignin; D006026:Glycoside Hydrolases
keywords: (hemi-)Cellulases; Aspergilli; Penicillia; Plant cell wall; Saccharification