title: Cleavage of Target DNA Promotes Sequence Conversion with a Tailed Duplex.
abstract: Base sequence conversion in target DNA is achieved when a 5'-tailed duplex (TD) is introduced into cells. In this study, the effects of target DNA cleavage on sequence conversion with a TD were examined. Plasmid DNAs with and without cleavage near the target position were each introduced into HeLa cells, together with the TD. The cleavage promoted the sequence alteration efficiency by ca. 7-fold. These results suggested that the sequence conversion efficiency with the TD fragment is increased when an artificial nuclease introduces cleavage near the target site.
authors.affiliation: Graduate School of Biomedical and Health Sciences, Hiroshima University.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Suzuki
authors.forename: Takashi
authors.initials: T
authors.lastname: Imada
authors.forename: Natsuki
authors.initials: N
authors.lastname: Nishigaki
authors.forename: Miwako
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Matsuoka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamiya
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids
chemical_list: D004247:DNA