title: Extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp.
abstract: In this research, extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp were investigated. A response surface methodology (RSM) with a Box-Behnken design (BBD) was used to optimize the extraction process. The maximum EAP yield was 9.82±0.38%, which is in good agreement with the predicted value (9.93±0.24%). Two homogeneous polysaccharides, EAP-1a and EAP-1b with molecular weights of 8.70kDa and 4.39kDa respectively, were prepared by DEAE-52 cellulose and Sephadex G-100 columns and characterized by HPLC, HPGPC, and FT-IR. Three polysaccharides (EAP, EAP-1a and EAP-1b) could stimulate macrophages to release NO and enhance phagocytic activities of RAW 264.7 cells in dose-dependent manner. Moreover, there was no significant difference between crude EAP group (400μg/mL) and positive control group (LPS) in effects on macrophages. The results implied that EAP had the potential to be developed as natural medicines or health foods.
authors.affiliation: College of Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China; College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China. Electronic address: duht@nwsuaf.edu.cn.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Du
authors.affiliation: College of Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China. Electronic address: cjc2009@nwsuaf.edu.cn.
authors.forename: Juncheng
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China. Electronic address: stian@nwsuaf.edu.cn.
authors.forename: Shan
authors.initials: S
authors.lastname: Tian
authors.affiliation: College of Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China. Electronic address: hlgu@nwsuaf.edu.cn.
authors.forename: Hongling
authors.initials: H
authors.lastname: Gu
authors.affiliation: College of Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China. Electronic address: lnuk@nwsuaf.edu.cn.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China. Electronic address: sunnyao07@163.com.
authors.forename: Yao
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China. Electronic address: Rujiajia2013@163.com.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Ru
authors.affiliation: College of Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China. Electronic address: wangjunru@nwsuaf.edu.cn.
authors.forename: Junru
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D002845:Chromatography / Q000379:methods*; D003911:Dextrans; D031263:Elaeagnaceae / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D011134:Polysaccharides / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology*; D000067996:RAW 264.7 Cells
chemical_list: D003911:Dextrans; D011134:Polysaccharides; C025614:sephadex
keywords: Characterization; Elaeagnus angustifolia L. pulp; Immunological activities; Optimized extraction; Polysaccharides