title: On-Line Solid-Phase Extraction Capillary Electrophoresis Mass Spectrometry for Preconcentration and Clean-Up of Peptides and Proteins.
abstract: One of the major drawbacks of capillary electrophoresis (CE) and other microscale separation techniques, for the analysis of low abundant peptides and proteins in complex samples, are the poor concentration limits of detection. Several strategies have been developed to improve CE sensitivity. Here, we describe an on-line solid-phase extraction capillary electrophoresis mass spectrometry method with a commercial C18 sorbent for clean-up and preconcentration of neuropeptides from highly diluted biological samples.
authors.affiliation: Department of Analytical Chemistry, University of Barcelona, Av. Diagonal 647, Barcelona, 08028, Spain. fbenavente@ub.edu.
authors.forename: Fernando
authors.initials: F
authors.lastname: Benavente
authors.affiliation: Department of Analytical Chemistry, University of Barcelona, Av. Diagonal 647, Barcelona, 08028, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Medina-Casanellas
authors.affiliation: Department of Analytical Chemistry, University of Barcelona, Av. Diagonal 647, Barcelona, 08028, Spain.
authors.forename: Estela
authors.initials: E
authors.lastname: Giménez
authors.affiliation: Department of Analytical Chemistry, University of Barcelona, Av. Diagonal 647, Barcelona, 08028, Spain.
authors.forename: Victoria
authors.initials: V
authors.lastname: Sanz-Nebot
mesh_terms: D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D009479:Neuropeptides / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000295:instrumentation* / Q000379:methods
chemical_list: D009479:Neuropeptides; D010455:Peptides; D011506:Proteins
keywords: Analyte concentrator; Capillary electrophoresis; Clean-up; In-line; Mass spectrometry; Microcartridge; On-line; Peptides; Preconcentration; Sensitivity; Solid phase extraction