title: Temporal profile of intestinal tissue expression of intestinal fatty acid-binding protein in a rat model of necrotizing enterocolitis.
abstract: OBJECTIVE
  Necrotizing enterocolitis is a severe multifactorial intestinal disorder that primarily affects preterm newborns, causing 20-40% mortality and morbidity. Intestinal fatty acid-binding protein has been reported to be a biomarker for the detection of intestinal injuries. Our aim was to assess intestinal tissue injury and the molecular expression of intestinal fatty acid-binding protein over time in a necrotizing enterocolitis model.
  
  
  METHODS
  A total of 144 Newborn rats were divided into two groups: 1) Control, which received breastfeeding (n=72) and 2) Necrotizing Enterocolitis, which received formula feeding and underwent hypoxia and hypothermia (n=72). A total of six time points of ischemia (2 times a day for 3 days; 12 pups for each time point) were examined. Samples were collected for analysis of body weight, morphological and histological characteristics, intestinal weight, intestinal weight/body weight ratio, injury grade, and intestinal fatty acid-binding protein levels.
  
  
  RESULTS
  Body and intestinal weights were lower in the Necrotizing Enterocolitis group than in the Control group (p<0.005 and p<0.0005, respectively). The intestinal weight/body weight ratio was higher in the Necrotizing Enterocolitis group than in the Control group (p<0.005) only at the sixth ischemia time point. The Necrotizing Enterocolitis group displayed higher expression of intestinal fatty acid-binding protein (p<0.0005) and showed greater tissue damage than the Control group.
  
  
  CONCLUSIONS
  Intestinal fatty acid-binding protein was an efficient marker of ischemic injury to the intestine and a good correlation was demonstrated between the time of ischemic injury and the grade of intestinal injury.
authors.affiliation: Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Divisão de Pediatria, Ribeirão Preto, SP/Brazil.
authors.forename: Ana Leda Bertoncini
authors.initials: AL
authors.lastname: Simões
authors.affiliation: Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Divisão de Pediatria, Ribeirão Preto, SP/Brazil.
authors.forename: Rebeca Lopes
authors.initials: RL
authors.lastname: Figueira
authors.affiliation: Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Divisão de Pediatria, Ribeirão Preto, SP/Brazil.
authors.forename: Frances Lilian Lanhellas
authors.initials: FL
authors.lastname: Gonçalves
authors.affiliation: Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Divisão de Pediatria, Ribeirão Preto, SP/Brazil.
authors.forename: Luís Felipe Tsuyoshi
authors.initials: LF
authors.lastname: Mitidiero
authors.affiliation: Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Cirurgia de Transplante, Ribeirão Preto, SP/Brazil.
authors.forename: Orlando Castro E
authors.initials: OC
authors.lastname: Silva
authors.affiliation: Fetal Care Center, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Peiró
authors.affiliation: Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Divisão de Pediatria, Ribeirão Preto, SP/Brazil.
authors.forename: Lourenço
authors.initials: L
authors.lastname: Sbragia
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D001835:Body Weight; D004195:Disease Models, Animal; D020345:Enterocolitis, Necrotizing / Q000378:metabolism* / Q000473:pathology*; D050556:Fatty Acid-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D000860:Hypoxia / Q000473:pathology; D007082:Ileum / Q000098:blood supply / Q000473:pathology*; D007150:Immunohistochemistry; D007511:Ischemia / Q000473:pathology; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012720:Severity of Illness Index; D013997:Time Factors
chemical_list: D015415:Biomarkers; D050556:Fatty Acid-Binding Proteins