title: Antimicrobial Resistance, Virulence Profile, and Molecular Characterization of Listeria monocytogenes Isolated from Ready-to-eat Food in China, 2013-2014.
abstract: We aimed to investigate the potential pathogenic profile and antibiotic resistance of Listeria monocytogenes isolated from ready-to-eat food in China. Antimicrobial resistance was determined by broth microdilution following the Clinical and Laboratory Standards Institute protocol. Molecular serotyping, virulence, and resistance genes were identified using PCR. Multi-locus sequence typing was performed on resistant strains. A total of 11.53% (113/980) isolates were resistant, from which 82.3% (93/113) harbored all the virulence genes tested. The resistant strains were subtyped into 18 sequence types (STs), from which ST2, ST5, ST8, and ST9 were involved in listeriosis. This study indicated that several L. monocytogenes isolates from ready-to-eat foods in China have pathogenic potential and are resistant to antibiotics, including antibiotics used as medicines by humans for listeriosis treatment.
authors.affiliation: Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Centre for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Shao Fei
authors.initials: SF
authors.lastname: Yan
authors.affiliation: Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Centre for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Centre for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Li
authors.initials: L
authors.lastname: Bai
authors.affiliation: Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Centre for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Yu Jie
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Centre for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Yin Ping
authors.initials: YP
authors.lastname: Dong
authors.affiliation: Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Centre for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Centre for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Feng Qin
authors.initials: FQ
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002681:China / Q000453:epidemiology; D024901:Drug Resistance, Multiple, Bacterial; D057140:Fast Foods / Q000382:microbiology*; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D008088:Listeriosis / Q000453:epidemiology* / Q000382:microbiology; D058885:Multilocus Sequence Typing; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents