title: Effect of low-level laser irradiation on proliferation of human dental mesenchymal stem cells; a systemic review.
abstract: BACKGROUND
  Identification of factors that enhance the proliferation of human dental mesenchymal stem cells (DMSCs) is vital to facilitate tissue regeneration. The role of low-level laser irradiation (LLLI) on proliferation of human DMSCs has not been well established.
  
  
  OBJECTIVE
  To assess the effect of LLLI on proliferation of human DMSCs when applied in-vitro.
  
  
  METHODS
  Electronic search of literature was conducted (2000-2016) on PubMed, Web of Science, and Scopus databases. Search terms included low-level light therapy, low-level laser irradiation, low-level light irradiation, LLLT, humans, adolescent, adult, cells, cultured, periodontal ligament, dental pulp, stem cells, dental pulp stem cells, mesenchymal stem cells, periodontal ligament stem cell, deciduous teeth, cell proliferation, adult stem cells, radiation, and proliferation.
  
  
  RESULTS
  The literature search identified 165 studies with 6 being eligible for inclusion; all used diode lasers; 5 studies used InGaAIP diode lasers; 4 used 660nm, and the other two applied 810nm or 980nm wavelength LLLI. The distance between the DMSCs and the laser spot ranged between 0.5mm to 2mm. The time intervals of cell proliferation analysis ranged from 0h to 7days after LLLI. After 660nm LLLI, an increase in the DMSC's proliferation was reported [DMSCs extracted from dental pulp of deciduous teeth (two irradiations, 3J/cm(2), 20mW was more effective than 40mW), adult teeth (two irradiations, 0.5 and 1.0J/cm(2), 30mW), and from adult periodontal ligament (two irradiations, 1.0J/cm(2) was more effective than 0.5J/cm(2), 30mW)]. Similarly, an increase in the proliferation of DMSCs extracted from dental pulp of adult teeth was reported after 810nm LLLI (7 irradiations in 7days, 0.1 and 0.2J/cm(2), 60mW) or 980nm LLLI (single irradiation, 3J/cm(2), 100mW). However, 660nm LLLI in one study did not increase the proliferation of DMSCs (single irradiation, energy densities of 0.05, 0.30, 7, and 42J/cm(2), 28mW).
  
  
  CONCLUSIONS
  There is limited evidence that in-vitro LLLI (660/810/980nm, with energy densities of 0.1-3J/cm(2)) increases the proliferation of DMSCs. Considering the limited evidence and their method heterogeneity it is difficult to reach a firm conclusion. Further research is necessary to identify the optimal characteristics of the LLLI setting (wave length, energy density, power output, frequency/duration of irradiations, distance between the cells and the laser spot/probe) to increase proliferation of DMSCs, and assess its impact on replicative senescence, as well as determine feasibility of the use in the clinical setting.
authors.affiliation: Orthodontics, Department of Clinical Sciences and Translational Medicine, Univeristy of Rome Tor Vergata, Rome, Italy; Warwick Medical School, University of Warwick, Coventry, and Specialist Orthodontic Practice, London, United Kingdom. Electronic address: faraortho@yahoo.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Borzabadi-Farahani
mesh_terms: D006801:Humans; D049109:Cell Proliferation / Q000528:radiation effects; D016208:Databases, Factual; D003804:Dentin / Q000166:cytology; D054023:Lasers, Semiconductor; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects
keywords: 660nm; 810nm; 980nm; Cell proliferation; Dental mesenchymal stem cells; Low-level laser irradiation