title: De Novo Transcriptome Assembly and Annotation of the Leaves and Callus of Cyclocarya Paliurus (Bata1) Iljinskaja.
abstract: Cyclocarya Paliurus (Bata1) Iljinskaja contains various bioactive secondary metabolites especially in leaves, such as triterpenes, flavonoids, polysaccharides and alkaloids, and its leaves are widely used as an hyperglycemic tea in China. In the present paper, we sequenced the transcriptome of the leaves and callus of Cyclocarya Paliurus using Illumina Hiseq 4000 platform. After sequencing and de novo assembly, a total of 65,654 unigenes were generated with an N50 length of 1,244bp. Among them, 35,041 (53.37%) unigenes were annotated in NCBI Non-Redundant database, 19,453 (29.63%) unigenes were classified into Gene Ontology (GO) database, and 7,259 (11.06%) unigenes were assigned to Clusters of Orthologous Group (COG) categories. Furthermore, 11,697 (17.81%) unigenes were mapped onto 335 pathways in Kyoto Encyclopedia of Genes and Genomes (KEGG), among which 1,312 unigenes were identified to be involved in biosynthesis of secondary metabolites. In addition, a total of 11,247 putative simple sequence repeats (SSRs) were detected. This transcriptome dataset provides a comprehensive sequence resource for gene expression profiling, genetic diversity, evolution and further molecular genetics research on Cyclocarya Paliurus.
authors.affiliation: Jiangxi Key Laboratory of Natural Products and Functional Food; Jiangxi Agricultural University, Nanchang 330045, China.|College of Food Science and engineering, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Xu
authors.affiliation: Jiangxi Key Laboratory of Natural Products and Functional Food; Jiangxi Agricultural University, Nanchang 330045, China.|College of Food Science and engineering, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Jiangxi Key Laboratory of Natural Products and Functional Food; Jiangxi Agricultural University, Nanchang 330045, China.|College of Food Science and engineering, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Jiguang
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin 300000, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Jiangxi Key Laboratory of Natural Products and Functional Food; Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Peng
authors.affiliation: Jiangxi Key Laboratory of Natural Products and Functional Food; Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Xinchen
authors.initials: X
authors.lastname: Shangguan
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis; D002874:Chromosome Mapping; D005419:Flavonoids / Q000096:biosynthesis; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D014644:Genetic Variation; D018745:Genome, Plant; D059014:High-Throughput Nucleotide Sequencing; D029525:Juglandaceae / Q000235:genetics* / Q000378:metabolism; D018895:Microsatellite Repeats; D058977:Molecular Sequence Annotation; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis; D064210:Secondary Metabolism / Q000235:genetics; D059467:Transcriptome; D014315:Triterpenes / Q000378:metabolism
chemical_list: D000470:Alkaloids; D005419:Flavonoids; D011134:Polysaccharides; D014315:Triterpenes