title: Recent developments in liquid chromatography-mass spectrometry analyses of ghrelin and related peptides.
abstract: Profiling and monitoring concentrations of key hormones in body have long been critical aims in clinical therapy. As a crucial hormone, identification and quantification of ghrelin is a fundamental, often key, step in understanding human physiological mechanisms. Through the advances and improvements of different analytical techniques, ghrelin measurement is generally feasible, and the number of successful reports is progressively being increased with new aspects of selectivity, sensitivity and ease of use in various circumstances. Herein we discuss current chromatographic methods for sample collection, separation and a mass spectrometry method for detection and measurement of ghrelin and other proghrelin-derived peptides in biological metrics. We describe the most commonly applied analytical LC-MS procedures for determination of proghrelin-derived peptides and provide illustrative instances representing the state of the art. This review is intended for bioanalytical chemists or clinical researchers who are interested in this field of research.
authors.affiliation: Faculty of Phytochemistry, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Evin, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Eslami
authors.affiliation: Faculty of Phytochemistry, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Evin, Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Ghassempour
authors.affiliation: Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Center, Dokki, Giza, 12622, Egypt.
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D054439:Ghrelin / Q000032:analysis* / Q000097:blood; D006801:Humans; D010455:Peptides / Q000032:analysis / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D054439:Ghrelin; D010455:Peptides
keywords: HPLC; acylated ghrelin; des-acyl ghrelin; mass spectrometry; proghrelin-derived peptides