title: PRDM5 promotes the proliferation and invasion of murine melanoma cells through up-regulating JNK expression.
abstract: PRDM (PRDI-BF1 and RIZ domain-containing) proteins constitute a family of zinc finger proteins and play important roles in multiple cellular processes by acting as epigenetic modifiers. PRDM5 is a recently identified member of the PRDM family and may function as a tumor suppressor in several types of cancer. However, the role of PRDM5 in murine melanoma remains largely unknown. In our study, effect of PRDM5 on murine melanoma cells was determined and results showed that PRDM5 overexpression significantly promoted proliferation, migration, and invasion of murine melanoma B16F10 cells. Consistently, silencing of PRDM5 expression significantly inhibited proliferation, invasion, and migration of B16F10 cells. In vivo study also showed that PRDM5 silencing significantly inhibited the growth and metastasis of melanoma in mice. PRDM5 was then found to increase the expression and activation of JNK in B16F10 cells. JNK silencing significantly reduced PRDM5-mediated up-regulation of JNK expression and blocked the PRDM5-induced proliferation and invasion of B16F10 cells. To further verify the involvement of JNK signaling in PRDM5-induced progression of B16F10 cells, a specific JNK inhibitor was employed to inhibit the JNK signaling pathway, and results showed that PRDM5-induced proliferation and invasion of B16F10 cells were abolished. We conclude that PRDM5 promotes the proliferation and invasion of murine melanoma cells through up-regulating JNK expression and strategies targeting PRDM5 may be promising for the therapy of melanoma.
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China.
authors.forename: Qiong-Qiong
authors.initials: QQ
authors.lastname: Ding
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Gao
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China.
authors.forename: Hai-Jie
authors.initials: HJ
authors.lastname: Yang
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China.
authors.forename: Mian
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Shi
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China.
authors.forename: Bin-Feng
authors.initials: BF
authors.lastname: Cheng
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China.
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Bi
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China. 131035@xxmu.edu.cn, xxyxy_fzw@163.com.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Feng
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D048031:JNK Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D020935:MAP Kinase Signaling System; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D008546:Melanoma, Experimental; D051379:Mice; D009362:Neoplasm Metastasis; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D047368:Tumor Burden
chemical_list: D004268:DNA-Binding Proteins; C575119:PRDM5 protein, mouse; D014157:Transcription Factors; D048031:JNK Mitogen-Activated Protein Kinases
keywords: JNK; PRDM5; melanoma; metastasis; proliferation