title: Long non-coding RNA XIST exerts oncogenic functions in human nasopharyngeal carcinoma by targeting miR-34a-5p.
abstract: Long non-coding RNA (lncRNA) X inactivate-specific transcript (XIST) has been verified as an oncogenic gene in several human malignant tumors, and its dysregulation was closed associated with tumor initiation, development and progression. Nevertheless, whether the aberrant expression of XIST in human nasopharyngeal carcinoma (NPC) is corrected with malignancy, metastasis or prognosis has not been elaborated. Here, we discovered that XIST was up-regulated in NPC tissues and higher expression of XIST contributed to a markedly poorer survival time. In addition, multivariate analysis demonstrated XIST was an independent risk factor for prognosis. XIST over-expression enhanced, while XIST silencing hampered the cell growth in NPC. Additionally, mechanistic analysis revealed that XIST up-regulated the expression of miR-34a-5p targeted gene E2F3 through acting as a competitive 'sponge' of miR-34a-5p. Taking all into account, we concluded that XIST functioned as an oncogene in NPC through up-regulating E2F3 in part through 'spongeing' miR-34a-5p.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Song
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei, PR China.
authors.forename: Lin-Feng
authors.initials: LF
authors.lastname: Ye
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei, PR China.
authors.forename: Cen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei, PR China. Electronic address: xhzwhu@sina.com.
authors.forename: Xu-Hong
authors.initials: XH
authors.lastname: Zhou
mesh_terms: D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D050699:E2F3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D050699:E2F3 Transcription Factor; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; C077885:XIST non-coding RNA
keywords: E2F3; Hsa-miRNA-34a-5p (miR-34a-5p); Nasopharyngeal carcinoma (NPC); Tumorigenesis; X inactivate-specific transcript (XIST)