title: The up-regulation of Myb may help mediate EGCG inhibition effect on mouse lung adenocarcinoma.
abstract: Green tea polyphenol epigallocatechin-3-gallate (EGCG) has been demonstrated to inhibit cancer in experimental studies through its antioxidant activity and modulations on cellular functions by binding specific proteins. By means of computational analysis and functional genomic approaches, we previously identified a set of protein coding genes and microRNAs whose expressions were significantly modulated in response to the EGCG treatment in tobacco carcinogen-induced lung adenocarcinoma in A/J mice. However, to what degree these genes are involved in the cancer inhibition of EGCG remains unclear.
  
  
  
  In this study, we further employed statistical methods and literature research to analyze these data in combination with The Cancer Genome Atlas (TCGA) lung adenocarcinoma datasets for additional data mining. Under the assumption that, if a gene mediates EGCG's cancer inhibition, its expression level change caused by EGCG should be opposite to what occurred in the carcinogenesis, we identified Myb and Peg3 as the primary putative genes involved in the cancer inhibitory activity. Further analysis suggested that the regulation of Myb could be mediated through an EGCG-upregulated microRNA, miR-449c-5p.
  
  
  
  Although the actions of EGCG involve multiple targets/pathways, further analysis by mining the existing genomic datasets revealed that the upregulations of Myb and Peg3 are likely the key anti-cancer events of EGCG in vivo.
authors.affiliation: Department of Mathematical Science, School of Health and Natural Sciences, University of Saint Joseph, 1678 Asylum Avenue, West Hartford, CT, 06117, USA. hzhou@usj.edu.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Mathematical Science, School of Health and Natural Sciences, University of Saint Joseph, 1678 Asylum Avenue, West Hartford, CT, 06117, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Manthey
authors.affiliation: Department of Mathematical Science, School of Health and Natural Sciences, University of Saint Joseph, 1678 Asylum Avenue, West Hartford, CT, 06117, USA.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Lioutikova
authors.affiliation: Joint Bioinformatics Ph.D. Program, University of Arkansas at Little Rock and University of Arkansas for Medical Sciences, 2801 S. University Avenue, Little Rock, AR, 72204, USA.
authors.forename: William
authors.initials: W
authors.lastname: Yang
authors.affiliation: Joint Bioinformatics Ph.D. Program, University of Arkansas at Little Rock and University of Arkansas for Medical Sciences, 2801 S. University Avenue, Little Rock, AR, 72204, USA.
authors.forename: Kenji
authors.initials: K
authors.lastname: Yoshigoe
authors.affiliation: Joint Bioinformatics Ph.D. Program, University of Arkansas at Little Rock and University of Arkansas for Medical Sciences, 2801 S. University Avenue, Little Rock, AR, 72204, USA. mqyang@ualr.edu.
authors.forename: Mary Qu
authors.initials: MQ
authors.lastname: Yang
authors.affiliation: Susan L. Cullman Laboratory for Cancer Research, Department of Chemical Biology and Centre for Cancer Prevention Research, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, 164 Frelinghuysen Road, Piscataway, NJ, 08854, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000235:genetics; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D053263:Gene Regulatory Networks; D051741:Kruppel-Like Transcription Factors / Q000235:genetics; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics; D008815:Mice, Inbred Strains; D035683:MicroRNAs / Q000235:genetics*; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D051741:Kruppel-Like Transcription Factors; D035683:MicroRNAs; C097901:Peg3 protein, mouse; D020598:Proto-Oncogene Proteins c-myb; D002392:Catechin; C045651:epigallocatechin gallate