title: VEGF and FGF2 Improve Revascularization, Survival, and Oocyte Quality of Cryopreserved, Subcutaneously-Transplanted Mouse Ovarian Tissues.
abstract: This study was conducted to investigate the effect of the vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF2) on revascularization, survival, and oocyte quality of cryopreserved, subcutaneously-transplanted mouse ovarian tissue. Autologous subcutaneous transplantation of vitrified-thawed mouse ovarian tissues treated with (experimental group) or without (control group) VEGF and FGF2 was performed. After transplantation to the inguinal region for two or three weeks, graft survival, angiogenesis, follicle development, and oocyte quality were examined after gonadotropin administration. VEGF coupled with FGF2 (VEGF/FGF2) promoted revascularization and significantly increased the survival rate of subcutaneously-transplanted cryopreserved ovarian tissues compared with untreated controls. The two growth factors did not show long-term effects on the ovarian grafts. In contrast to the untreated ovarian grafts, active folliculogenesis was revealed as the number of follicles at various stages and of mature oocytes in antral follicles after gonadotropin administration were remarkably higher in the VEGF/FGF2-treated groups. Although the fertilization rate was similar between the VEGF/FGF2 and control groups, the oocyte quality was much better in the VEGF/FGF2-treated grafts as demonstrated by the higher ratio of blastocyst development. Introducing angiogenic factors, such as VEGF and FGF2, may be a promising strategy to improve revascularization, survival, and oocyte quality of cryopreserved, subcutaneously-transplanted mouse ovarian tissue.
authors.affiliation: Department of Medical Research, Mackay Memorial Hospital, Tamsui District, New Taipei City 251, Taiwan. lsh@mmh.org.tw.|Mackay Junior College of Medicine, Nursing, and Management, Beitou District, Taipei City 112, Taiwan. lsh@mmh.org.tw.
authors.forename: Sheng-Hsiang
authors.initials: SH
authors.lastname: Li
authors.affiliation: Department of Medical Research, Mackay Memorial Hospital, Tamsui District, New Taipei City 251, Taiwan. hwu4416@yahoo.com.tw.|Mackay Junior College of Medicine, Nursing, and Management, Beitou District, Taipei City 112, Taiwan. hwu4416@yahoo.com.tw.|Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 104, Taiwan. hwu4416@yahoo.com.tw.|Mackay Medical College, Sanzhi District, New Taipei City 252, Taiwan. hwu4416@yahoo.com.tw.
authors.forename: Yuh-Ming
authors.initials: YM
authors.lastname: Hwu
authors.affiliation: Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 104, Taiwan. d95642001@gmail.com.
authors.forename: Chung-Hao
authors.initials: CH
authors.lastname: Lu
authors.affiliation: Department of Medical Research, Mackay Memorial Hospital, Tamsui District, New Taipei City 251, Taiwan. smallriver220@gmail.com.
authors.forename: Hsiao-Ho
authors.initials: HH
authors.lastname: Chang
authors.affiliation: Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 104, Taiwan. shitxn@gmail.com.
authors.forename: Cheng-En
authors.initials: CE
authors.lastname: Hsieh
authors.affiliation: Department of Medical Research, Mackay Memorial Hospital, Tamsui District, New Taipei City 251, Taiwan. mmh40@mmh.org.tw.|Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 104, Taiwan. mmh40@mmh.org.tw.|Department of Obstetrics and Gynecology, Taipei Medical University, Taipei City 110, Taiwan. mmh40@mmh.org.tw.
authors.forename: Robert Kuo-Kuang
authors.initials: RK
authors.lastname: Lee
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D015925:Cryopreservation; D047108:Embryonic Development / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005307:Fertilization in Vitro; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007124:Immunoenzyme Techniques; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D010053:Ovary / Q000166:cytology* / Q000187:drug effects; D040521:Subcutaneous Tissue; D014182:Transplantation, Autologous; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2
keywords: angiogenic factor; fertility reservation; oocyte quality; ovarian cryopreservation; ovarian transplantation