title: Integrative taxonomy of Anisakidae and Raphidascarididae (Nematoda) in Paralichthys patagonicus and Xystreurys rasile (Pisces: Teleostei) from Brazil.
abstract: Thirty-six Paralichthys patagonicus and 30 Xystreurys rasile were collected in the state of Rio de Janeiro, Brazil to investigate the presence of anisakid and raphidascaridid nematodes. Anisakis typica, Terranova sp., Contracaecum sp., Hysterothylacium deardorffoverstreetorum, and Raphidascaris sp. were identified using integrative taxonomy of morphological and genetic data. Morphological and morphometric analysis was conducted using bright field microscopy with scanning electron microscopy for topographic characterization of the cuticular surface. Phylogenetic analysis, using ITS and cox2 molecular targets, clearly demonstrated the species identification of A. typica and H. deardorffoverstreetorum and the high diversity of H. deardorffoverstreetorum. This is the first report of A. typica, H. deardorffoverstreetorum, and Raphidascaris sp. parasitizing P. patagonicus and X. rasile.
authors.affiliation: Laboratório de Inspeção e Tecnologia do Pescado, Faculdade de Medicina Veterinária, Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, Niterói, RJ 24230-340, Brazil.
authors.forename: Michelle Cristie Gonçalves da
authors.initials: MC
authors.lastname: Fonseca
authors.affiliation: Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, RJ 21040-900, Brazil. Electronic address: knoffm@ioc.fiocruz.br.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Knoff
authors.affiliation: Laboratório de Inspeção e Tecnologia do Pescado, Faculdade de Medicina Veterinária, Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, Niterói, RJ 24230-340, Brazil.
authors.forename: Nilza Nunes
authors.initials: NN
authors.lastname: Felizardo
authors.affiliation: LABTRIP, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, RJ 21045-900, Brazil.
authors.forename: Maria Isabel N
authors.initials: MI
authors.lastname: Di Azevedo
authors.affiliation: Laboratório de Helmintologia Romero Lascasas Porto, Departamento de Microbiologia, Imunologia e Parasitologia, Escola de Ciências Médicas, Universidade Estadual do Rio de Janeiro, Rua Prof. Manuel de Abreu 444, 5° andar, Vila Isabel, Rio de Janeiro, RJ 20550-170, Brazil.
authors.forename: Eduardo José Lopes
authors.initials: EJ
authors.lastname: Torres
authors.affiliation: Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, RJ 21040-900, Brazil.
authors.forename: Delir Corrêa
authors.initials: DC
authors.lastname: Gomes
authors.affiliation: LABTRIP, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, RJ 21045-900, Brazil.
authors.forename: Alena Mayo
authors.initials: AM
authors.lastname: Iñiguez
authors.affiliation: Laboratório de Inspeção e Tecnologia do Pescado, Faculdade de Medicina Veterinária, Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, Niterói, RJ 24230-340, Brazil.
authors.forename: Sérgio Carmona de
authors.initials: SC
authors.lastname: São Clemente
mesh_terms: D000818:Animals; D017163:Anisakis / Q000033:anatomy & histology* / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001938:Brazil; D051546:Cyclooxygenase 2 / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005393:Fish Diseases / Q000469:parasitology*; D005432:Flounder / Q000469:parasitology*; D008855:Microscopy, Electron, Scanning; D010802:Phylogeny
chemical_list: D021903:DNA, Ribosomal Spacer; D051546:Cyclooxygenase 2
keywords: Anisakidae; Brazil; Nematodes; Paralichthys patagonicus; Raphidascarididae; Xystreurys rasile