title: Septin crystallization for structural analysis.
abstract: Septins are filament-forming proteins found in many eukaryotes. Despite being important components of the cytoskeleton, only recently details of their macromolecular assemblies and crystal structures have started to appear in the literature. These are of fundamental importance to the understanding of cytoskeleton dynamics, membrane barrier formation, and bacterial caging, as well as essential cellular processes such as cell division, exocytosis, and vesicle trafficking. However, obtaining this data is frequently hindered by several experimental difficulties common to the majority of septin samples. Here we provide an overview of the current approaches to circumvent or minimize the experimental complications observed in septin crystallography focusing mainly, but not exclusively, on the choice of the septin construct and how to best prepare the sample itself.
authors.affiliation: University of Brasília, Brasília, Brazil; University of São Paulo, São Carlos, Brazil.
authors.forename: N F
authors.initials: NF
authors.lastname: Valadares
authors.affiliation: University of Brasília, Brasília, Brazil; University of São Paulo, São Carlos, Brazil.
authors.forename: R C
authors.initials: RC
authors.lastname: Garratt
mesh_terms: D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods*; D003599:Cytoskeleton / Q000737:chemistry; D005089:Exocytosis / Q000235:genetics; D008870:Microtubules / Q000737:chemistry; D058112:Septins / Q000737:chemistry*
chemical_list: D058112:Septins
keywords: Construct; Crystallization; Crystallography; Protein structure; Sample preparation; Septins