title: Multi-layered nanofibrous mucoadhesive films for buccal and sublingual administration of drug-delivery and vaccination nanoparticles - important step towards effective mucosal vaccines.
abstract: Nanofibre-based mucoadhesive films were invented for oromucosal administration of nanocarriers used for delivery of drugs and vaccines. The mucoadhesive film consists of an electrospun nanofibrous reservoir layer, a mucoadhesive film layer and a protective backing layer. The mucoadhesive layer is responsible for tight adhesion of the whole system to the oral mucosa after application. The electrospun nanofibrous reservoir layer is intended to act as a reservoir for polymeric and lipid-based nanoparticles, liposomes, virosomes, virus-like particles, dendrimers and the like, plus macromolecular drugs, antigens and/or allergens. The extremely large surface area of nanofibrous reservoir layers allows high levels of nanoparticle loading. Nanoparticles can either be reversibly adsorbed to the surface of nanofibres or they can be deposited in the pores between the nanofibres. After mucosal application, nanofibrous reservoir layers are intended to promote prolonged release of nanoparticles into the submucosal tissue. Reversible adsorption of model nanoparticles as well as sufficient mucoadhesive properties were demonstrated. This novel system appears appropriate for the use in oral mucosa, especially for sublingual and buccal tissues. To prove this concept, trans-/intramucosal and lymph-node delivery of PLGA-PEG nanoparticles was demonstrated in a porcine model. This system can mainly be used for sublingual immunization and the development of "printed vaccine technology".
authors.affiliation: Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Brno, Czech Republic. Electronic address: masek@vri.cz.
authors.forename: Josef
authors.initials: J
authors.lastname: Mašek
authors.affiliation: Technical University of Liberec, Institute for Nanomaterials, Advanced Technologies and Innovation, Liberec, Czech Republic.
authors.forename: Daniela
authors.initials: D
authors.lastname: Lubasová
authors.affiliation: Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Róbert
authors.initials: R
authors.lastname: Lukáč
authors.affiliation: Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Pavlína
authors.initials: P
authors.lastname: Turánek-Knotigová
authors.affiliation: Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kulich
authors.affiliation: Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Plocková
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Eliška
authors.initials: E
authors.lastname: Mašková
authors.affiliation: Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Lubomír
authors.initials: L
authors.lastname: Procházka
authors.affiliation: Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Štěpán
authors.initials: Š
authors.lastname: Koudelka
authors.affiliation: Technical University of Liberec, Institute for Nanomaterials, Advanced Technologies and Innovation, Liberec, Czech Republic.
authors.forename: Nongnut
authors.initials: N
authors.lastname: Sasithorn
authors.affiliation: Leica Microsystems, Heerbrugg, Switzerland.
authors.forename: Jozsef
authors.initials: J
authors.lastname: Gombos
authors.affiliation: Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Eliška
authors.initials: E
authors.lastname: Bartheldyová
authors.affiliation: Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Brno, Czech Republic.
authors.forename: František
authors.initials: F
authors.lastname: Hubatka
authors.affiliation: Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Brno, Czech Republic; Department of Immunology, Palacky University, Olomouc, Czech Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Raška
authors.affiliation: Institute of Pharmaceutical Science, King's College London, London, United Kingdom; KP Therapeutics Ltd, 86 Deansgate, Manchester, M3 2ER, United Kingdom.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Miller
authors.affiliation: Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Brno, Czech Republic. Electronic address: turanek@vri.cz.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Turánek
mesh_terms: D000269:Adhesives / Q000737:chemistry; D000278:Administration, Buccal; D000286:Administration, Sublingual; D000818:Animals; D016503:Drug Delivery Systems / Q000379:methods*; D008081:Liposomes / Q000737:chemistry; D008198:Lymph Nodes / Q000378:metabolism; D051379:Mice; D009061:Mouth Mucosa / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*; D011092:Polyethylene Glycols / Q000737:chemistry; D011098:Polyglactin 910 / Q000737:chemistry; D013552:Swine; D014611:Vaccination / Q000379:methods; D014612:Vaccines / Q000008:administration & dosage*
chemical_list: D000269:Adhesives; D008081:Liposomes; D004364:Pharmaceutical Preparations; D014612:Vaccines; C102418:poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer; D011098:Polyglactin 910; D011092:Polyethylene Glycols
keywords: drug delivery; electrospinning; mucoadhesive film; mucus penetrating nanoparticles; nanofibres; sublingual vaccines