title: Chemical Composition and Inhibitory Effect of Lentinula edodes Ethanolic Extract on Experimentally Induced Atopic Dermatitis in Vitro and in Vivo.
abstract: The ethanolic extract of Lentinula edodes was partially analyzed and then characterized for its efficacy in treating atopic dermatitis. Polyphenols were determined to be the major antioxidant component in the extract (6.12 mg/g), followed by flavonoids (1.76 mg/g), β-carotene (28.75 μg/g), and lycopene (5.25 μg/g). An atopic dermatitis (AD) model was established and epidermal and dermal ear thickness, mast cell infiltration, and serum immunoglobulin levels were measured after oral administration of the L. edodes extract for 4 weeks. L. edodes extract decreased Dermatophagoides farinae extract (DFE) and 4-dinitrochlorobenzene (DNCB)-induced expression of several inflammatory cytokines in the ears, cervical lymph nodes, and splenocytes. Consequently, L. edodes extract may have therapeutic potential in the treatment of AD attributable to its immunomodulatory effects.
authors.affiliation: Division of Sport Science, College of Science and Technology, Konkuk University, Chungju 27478, Korea. ooj7990@kku.ac.kr.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju 61005, Korea. zeeyong@gist.ac.kr.
authors.forename: Zee-Yong
authors.initials: ZY
authors.lastname: Park
authors.affiliation: Division of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Korea. eunkyungkim@kku.ac.kr.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D040001:Dermatophagoides farinae; D004137:Dinitrochlorobenzene / Q000633:toxicity; D004195:Disease Models, Animal; D000431:Ethanol / Q000737:chemistry*; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D020942:Shiitake Mushrooms / Q000737:chemistry*
chemical_list: D004137:Dinitrochlorobenzene; D007155:Immunologic Factors; D010936:Plant Extracts; D000431:Ethanol
keywords: Lentinula edodes; atopic dermatitis; immunomodulatory; inflammatory cytokines