title: Method to Screen Multidrug Transport Inhibitors Using Yeast Overexpressing a Human MDR Transporter.
abstract: Multidrug resistance has appeared to mitigate the efficiency of anticancer drugs and the possibility of successful cancer chemotherapy. The Hedgehog receptor Patched is a multidrug transporter expressed in several cancers and as such it represents a new target to circumvent chemotherapy resistance. In this chapter, we describe the screening test developed to identify molecules able to inhibit the drug efflux activity of Patched. This screening test uses yeast overexpressing functional human Patched that have been shown to resist to chemotherapeutic agents. This test can be adapted to other MDR transporters.
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, UMR 7275 CNRS, Université de Nice-Sophia Antipolis, 660 route des Lucioles, 06560, Sophia Antipolis, Valbonne, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Fiorini
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, UMR 7275 CNRS, Université de Nice-Sophia Antipolis, 660 route des Lucioles, 06560, Sophia Antipolis, Valbonne, France. mus-veteau@ipmc.cnrs.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Mus-Veteau
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology; D018432:Drug Resistance, Multiple / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D015870:Gene Expression; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D000072079:Patched Receptors / Q000037:antagonists & inhibitors*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development
chemical_list: D000970:Antineoplastic Agents; D000072079:Patched Receptors; D004317:Doxorubicin
keywords: Chemotherapy resistance; Membrane protein; Multidrug transporters; Overexpression in yeast; Patched; Screening test