title: Phytochemical study and antioxidant, antimicrobial and anticancer activities of Melanelia subaurifera and Melanelia fuliginosa lichens.
abstract: The aim of this study was to investigate antioxidant, antimicrobial and anticancerous activity of Melanelia subaurifera and Melanelia fuliginosa. The phytochemical analysis was determined by HPLC-UV method. Antioxidant activity was evaluated by DPPH and reducing power assay while antimicrobial activity was determined by minimal inhibitory concentration. The cytotoxic activity was tested using MTT method. The method for quantification of 2'-O-methyl anziaic acid and lecanoric acid in these lichens using RF-HPLC was also developed and validated. The depsides (lecanoric acid, gyrophoric acid, atranorin, anziaic acid and 2'-O-methyl anziaic acid), and dibenzofurane (usnic acid) were identified in these lichens. The antioxidant activity (IC50) of lichens extracts ranged from 121.52 to 424.51 μg/ml. 2'-O-Methyl anziaic acid showed the highest antimicrobial activity with MIC ranging from 0.0625 to 1 mg/ml. M. subaurifera extract showed the highest cytotoxic activity against the tested cell lines (IC50 = 9.88 to 31.64 μg/ml).
authors.affiliation: Department of Biology, Faculty of Science, University of Kragujevac, Kragujevac, 34000 Serbia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Ristić
authors.affiliation: Department of Biology, Faculty of Science, University of Kragujevac, Kragujevac, 34000 Serbia.
authors.forename: Branislav
authors.initials: B
authors.lastname: Ranković
authors.affiliation: Department of Biology, Faculty of Science, University of Kragujevac, Kragujevac, 34000 Serbia.
authors.forename: Marijana
authors.initials: M
authors.lastname: Kosanić
authors.affiliation: Institute of Oncology and Radiology of Serbia, Belgrade, 11000 Serbia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Stanojković
authors.affiliation: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Nis, 18000 Serbia.
authors.forename: Slaviša
authors.initials: S
authors.lastname: Stamenković
authors.affiliation: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Nis, 18000 Serbia.
authors.forename: Perica
authors.initials: P
authors.lastname: Vasiljević
authors.affiliation: Department of Pharmacy, Faculty of Medicinal Sciences, University of Kragujevac, Kragujevac, 34000 Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Manojlović
authors.affiliation: Department of Pharmacy, Faculty of Medicinal Sciences, University of Kragujevac, Kragujevac, 34000 Serbia.
authors.forename: Nedeljko
authors.initials: N
authors.lastname: Manojlović
keywords: Biological activities; HPLC analysis; Lichens secondary metabolites