title: Gastric submucosa is inferior to the liver as transplant site for autologous islet transplantation in pancreatectomized diabetic Beagles.
abstract: Intraportal transplantation of islets is no longer considered to be an ideal procedure and finding the extrahepatic alternative site is becoming a subject of high priority. Herein, in this study, we would introduce our initial outcomes of using gastric submucosa (GS) and liver as sites of islet autotransplantation in pancreatectomized diabetic Beagles. Total pancreatectomy was performed in Beagles and then their own islets extracted from the excised pancreas were transplanted into GS (GS group, n=8) or intrahepatic via portal vein (PV group, n=5). Forty-eight hours post transplantation, graft containing tissue harvested from the recipients revealed the presence of insulin-positive cells. All recipients in GS group achieved euglycemia within 1 day, but returned to a diabetic state at 6 to 8 days post-transplantation (mean survival time, 7.16±0.69 days). However, all of the animals kept normoglycemic until 85 to 155 days post-transplantation in PV group (mean survival time, 120±28.58 days; P<0.01 vs. GS group). The results of intravenous glucose tolerance test (IVGTT) confirmed that the marked improvement in glycometabolism was obtained in intrahepatic islet autotransplantation. Thus, our findings indicate that the liver is still superior to the GS as the site of islet transplantation, at least in our islet autotransplant model in pancreatectomized diabetic Beagles.
authors.affiliation: Department of Hepatopancreaticobiliary Surgical Oncology, Chinese People's Liberation Army (PLA) General Hospital, Beijing, 100853, China.
authors.forename: Zhu-Zeng
authors.initials: ZZ
authors.lastname: Yin
authors.affiliation: Department of Organ Transplantation, Tianjin First Center Hospital, Tianjin, 300192, China.
authors.forename: Shu-Sen
authors.initials: SS
authors.lastname: Wang
authors.affiliation: Dparment of General Surgery, Tianjin Union Medicine Center, Tianjin, 300121, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Dparment of General Surgery, Tianjin Union Medicine Center, Tianjin, 300121, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Dparment of General Surgery, Tianjin Union Medicine Center, Tianjin, 300121, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.|Key Laboratory of Organ Transplantation, Ministry of Education, Wuhan, 430030, China.|Key Laboratory of Organ Transplantation, Ministry of Public Health, Wuhan, 430030, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Hepatopancreaticobiliary Surgical Oncology, Chinese People's Liberation Army (PLA) General Hospital, Beijing, 100853, China. liurong301@126.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Tianjin Hospital of ITCWM, Nankai Hospital, Tianjin, 300100, China. wangximo@126.com.
authors.forename: Xi-Mo
authors.initials: XM
authors.lastname: Wang
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D004285:Dogs; D005753:Gastric Mucosa / Q000378:metabolism / Q000637:transplantation*; D005947:Glucose / Q000378:metabolism; D005951:Glucose Tolerance Test; D006085:Graft Survival; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation; D008099:Liver / Q000473:pathology; D016031:Liver Transplantation; D014182:Transplantation, Autologous
chemical_list: D007328:Insulin; D005947:Glucose
keywords: Beagle; gastric submucosa; islet autotransplantation; portal vein