title: In situ thiolated alginate hydrogel: Instant formation and its application in hemostasis.
abstract: An in situ formed hydrogel was synthesized by sodium alginate and cysteine methyl ester, which turned the sodium alginate into thiolated alginate (SA-SH). SA-SH can in situ formed into hydrogel (SA-SS-SA) with a large amount of water through covalent bond in less than 20 s. The structure characterization showed that the mechanism of SA-SH gelation was thiol-disulfide transformation. The rheology and cytotoxicity experiments of SA-SS-SA hydrogel were also investigated, which indicated that SA-SS-SA hydrogel had an appropriate mechanical strength as well as an excellent biocompatibility. The SA-SS-SA hydrogel would degrade under certain conditions after a few days and its mechanism was disulfide alkaline reduction. Finally, the hemostatic property of SA-SH was tested by rat tail amputation experiment. The time to hemostasis of rat reduced from 8.26 min to 3.24 min, which proved that SA-SH had an excellent hemostatic property.
authors.affiliation: Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai China.
authors.forename: Guanzhe
authors.initials: G
authors.lastname: Xu
authors.affiliation: Shanghai No. 6 People's Hospital, Shanghai, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai China.
authors.forename: Qintong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai China.
authors.forename: Changlin
authors.initials: C
authors.lastname: Chen
authors.affiliation: Collaborative Innovation Center for Petrochemical New Materials, Anqing, Anhui, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Xu
authors.affiliation: Shanghai No. 6 People's Hospital, Shanghai, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai China mdlang@ecust.edu.cn.
authors.forename: Meidong
authors.initials: M
authors.lastname: Lang
mesh_terms: D000464:Alginates / Q000008:administration & dosage* / Q000138:chemical synthesis*; D000818:Animals; D001458:Bandages; D019245:Compressive Strength; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000138:chemical synthesis; D006487:Hemostasis / Q000187:drug effects* / Q000502:physiology; D006490:Hemostatics / Q000008:administration & dosage* / Q000138:chemical synthesis*; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000138:chemical synthesis; D020100:Hydrogels / Q000008:administration & dosage / Q000138:chemical synthesis*; D008422:Materials Testing; D051381:Rats; D013438:Sulfhydryl Compounds / Q000008:administration & dosage / Q000138:chemical synthesis; D016896:Treatment Outcome; D014783:Viscosity
chemical_list: D000464:Alginates; D006490:Hemostatics; D006603:Hexuronic Acids; D020100:Hydrogels; D013438:Sulfhydryl Compounds; D020723:Glucuronic Acid
keywords: Sodium alginate; cysteine methyl ester hydrochloride; hemostasis; in situ hydrogel; wound healing