title: HP1BP3 expression determines maternal behavior and offspring survival.
abstract: Maternal care is an indispensable behavioral component necessary for survival and reproductive success in mammals, and postpartum maternal behavior is mediated by an incompletely understood complex interplay of signals including effects of epigenetic regulation. We approached this issue using our recently established mice with targeted deletion of heterochromatin protein 1 binding protein 3 (HP1BP3), which we found to be a novel epigenetic repressor with critical roles in postnatal growth. Here, we report a dramatic reduction in the survival of pups born to Hp1bp3(-/-) deficient mouse dams, which could be rescued by co-fostering with wild-type dams. Hp1bp3(-/-) females failed to retrieve both their own pups and foster pups in a pup retrieval test, and showed reduced anxiety-like behavior in the open-field and elevated-plus-maze tests. In contrast, Hp1bp3(-/-) females showed no deficits in behaviors often associated with impaired maternal care, including social behavior, depression, motor coordination and olfactory capability; and maintained unchanged anxiety-associated hallmarks such as cholinergic status and brain miRNA profiles. Collectively, our results suggest a novel role for HP1BP3 in regulating maternal and anxiety-related behavior in mice and call for exploring ways to manipulate this epigenetic process.
authors.affiliation: The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel. benny.garfinkel@mail.huji.ac.il.|Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel. benny.garfinkel@mail.huji.ac.il.
authors.forename: B P
authors.identifier: 0000-0002-2790-1349
authors.initials: BP
authors.lastname: Garfinkel
authors.affiliation: Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.|Biomedical Sciences, The Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Arad
authors.affiliation: Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, TN, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Neuner
authors.affiliation: Sagol Department of Neurobiology, University of Haifa, Haifa, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Netser
authors.affiliation: Sagol Department of Neurobiology, University of Haifa, Haifa, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Wagner
authors.affiliation: Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, TN, USA.
authors.forename: C C
authors.initials: CC
authors.lastname: Kaczorowski
authors.affiliation: Center for Clinical and Translational Research, Maine Medical Center Research Institute, Scarborough, ME, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Rosen
authors.affiliation: Biomedical Sciences, The Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.|The IVF Unit - Obstetrics and Gynecology Department, Shaare Zedek Medical Center, Jerusalem, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Gal
authors.affiliation: The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.|Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Soreq
authors.affiliation: Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: J
authors.initials: J
authors.lastname: Orly
mesh_terms: D000818:Animals; D001007:Anxiety / Q000235:genetics / Q000378:metabolism; D001522:Behavior, Animal / Q000502:physiology; D001921:Brain / Q000378:metabolism; D003863:Depression / Q000235:genetics / Q000378:metabolism; D044127:Epigenesis, Genetic; D005260:Female; D008425:Maternal Behavior / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy; D012098:Reproduction / Q000502:physiology; D012919:Social Behavior
chemical_list: C000598191:HP1BP3 protein, mouse; D009687:Nuclear Proteins
keywords: Anxiety; HP1BP3; chromatin; epigenetics; histone; knockout mouse; maternal behavior; microRNA; social; survival