title: Green tea (-)-epigallocatechin-3-gallate counteracts daytime overeating induced by high-fat diet in mice.
abstract: High-fat diet (HFD) induces overeating and obesity. Green tea (-)-epigallocatechin-3-gallate (EGCG) reduces HFD-induced body weight and body fat gain mainly through increased lipid metabolism and fat oxidation. However, little is known about its effect on HFD-induced alterations in feeding behavior.
  
  
  
  Three diet groups of wildtype C57B/6j male mice at 5 months old were fed on normal chow diet, 1 week of HFD (60% of energy) and 3 months of HFD (diet-induced obesity (DIO)) prior to EGCG supplement in respective diet. EGCG had no effect on feeding behavior in normal chow diet group. Increased daytime feeding induced by HFD was selectively corrected by EGCG treatment in HFD groups, including reversed food intake, feeding frequency and meal size in HFD + EGCG group, and reduced food intake and feeding frequency in DIO + EGCG group. Moreover, EGCG treatment altered diurnally oscillating expression pattern of key appetite-regulating genes, including AGRP, POMC, and CART, and key circadian genes Clock and Bmal1 in hypothalamus of DIO mice, indicating its central effect on feeding regulation.
  
  
  
  Our study demonstrates that EGCG supplement specifically counteracts daytime overeating induced by HFD in mice, suggesting its central role in regulating feeding behavior and energy homeostasis.
authors.affiliation: Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.affiliation: Abbott Nutrition, Abbott Laboratories (Singapore) Pte Ltd, Singapore.
authors.forename: Huiling Calvina
authors.initials: HC
authors.lastname: Kek
authors.affiliation: Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore.
authors.forename: Joy
authors.initials: J
authors.lastname: Lim
authors.affiliation: Abbott Nutrition, Abbott Laboratories (Singapore) Pte Ltd, Singapore.|Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Richard Wayne
authors.initials: RW
authors.lastname: Gelling
authors.affiliation: Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore.
authors.forename: Weiping
authors.initials: W
authors.lastname: Han
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D059305:Diet, High-Fat / Q000009:adverse effects; D004195:Disease Models, Animal; D005247:Feeding Behavior / Q000187:drug effects*; D006706:Homeostasis; D006963:Hyperphagia / Q000517:prevention & control*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000517:prevention & control; D013662:Tea / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Appetite; Circadian rhythms; EGCG; Feeding behavior; HFD; Obesity