title: Comparative genomic analysis of Lactobacillus plantarum ZJ316 reveals its genetic adaptation and potential probiotic profiles.
abstract: OBJECTIVE
  In previous studies, Lactobacillus plantarum ZJ316 showed probiotic properties, such as antimicrobial activity against various pathogens and the capacity to significantly improve pig growth and pork quality. The purpose of this study was to reveal the genes potentially related to its genetic adaptation and probiotic profiles based on comparative genomic analysis.
  
  
  METHODS
  The genome sequence of L. plantarum ZJ316 was compared with those of eight L. plantarum strains deposited in GenBank. BLASTN, Mauve, and MUMmer programs were used for genome alignment and comparison. CRISPRFinder was applied for searching the clustered regularly interspaced short palindromic repeats (CRISPRs).
  
  
  RESULTS
  We identified genes that encode proteins related to genetic adaptation and probiotic profiles, including carbohydrate transport and metabolism, proteolytic enzyme systems and amino acid biosynthesis, CRISPR adaptive immunity, stress responses, bile salt resistance, ability to adhere to the host intestinal wall, exopolysaccharide (EPS) biosynthesis, and bacteriocin biosynthesis.
  
  
  CONCLUSIONS
  Comparative characterization of the L. plantarum ZJ316 genome provided the genetic basis for further elucidating the functional mechanisms of its probiotic properties. ZJ316 could be considered a potential probiotic candidate.
authors.affiliation: Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.|College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Gu
authors.affiliation: Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
authors.forename: Xiu-Yu
authors.initials: XY
authors.lastname: Lou
authors.affiliation: Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
authors.forename: Da-Feng
authors.initials: DF
authors.lastname: Song
authors.affiliation: Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis; D001422:Bacterial Adhesion; D016667:Bacterial Capsules / Q000378:metabolism; D001430:Bacteriocins / Q000096:biosynthesis; D002244:Carbon / Q000378:metabolism; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D048191:Lactiplantibacillus plantarum / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D019936:Probiotics
chemical_list: D000596:Amino Acids; D001430:Bacteriocins; D002244:Carbon
keywords: Adaptation; Comparative genomics; Lactobacillus plantarum ZJ316; Probiotics