title: RGDS- and SIKVAVS-Modified Superporous Poly(2-hydroxyethyl methacrylate) Scaffolds for Tissue Engineering Applications.
abstract: Three-dimensional hydrogel supports for mesenchymal and neural stem cells (NSCs) are promising materials for tissue engineering applications such as spinal cord repair. This study involves the preparation and characterization of superporous scaffolds based on a copolymer of 2-hydroxyethyl and 2-aminoethyl methacrylate (HEMA and AEMA) crosslinked with ethylene dimethacrylate. Ammonium oxalate is chosen as a suitable porogen because it consists of needle-like crystals, allowing their parallel arrangement in the polymerization mold. The amino group of AEMA is used to immobilize RGDS and SIKVAVS peptide sequences with an N-γ-maleimidobutyryloxy succinimide ester linker. The amount of the peptide on the scaffold is determined using125I radiolabeled SIKVAVS. Both RGDS- and SIKVAVS-modified poly(2-hydroxyethyl methacrylate) scaffolds serve as supports for culturing human mesenchymal stem cells (MSCs) and human fetal NSCs. The RGDS sequence is found to be better for MSC and NSC proliferation and growth than SIKVAVS.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06, Prague 6, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Macková
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06, Prague 6, Czech Republic.
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Plichta
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06, Prague 6, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Proks
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06, Prague 6, Czech Republic.
authors.forename: Ilya
authors.initials: I
authors.lastname: Kotelnikov
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06, Prague 6, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Kučka
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06, Prague 6, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Hlídková
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06, Prague 6, Czech Republic.
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Vídenˇská 1083, 14220, Prague 4, Czech Republic.
authors.forename: Šárka
authors.initials: Š
authors.lastname: Kubinová
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Vídenˇská 1083, 14220, Prague 4, Czech Republic.
authors.forename: Klára
authors.initials: K
authors.lastname: Jiráková
mesh_terms: D002460:Cell Line; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008768:Methylmethacrylates / Q000737:chemistry / Q000494:pharmacology; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D008768:Methylmethacrylates; D009842:Oligopeptides; C077583:seryl-isoleucyl-lysyl-valyl-alanyl-valinamide; C021302:hydroxymethylmethacrylate; C046617:arginyl-glycyl-aspartyl-serine
keywords: IKVAV; RGD; peptide; poly(2-hydroxyethyl methacrylate); stem cell