title: Evaluation of antidiabetic effect of total calystegines extracted from Hyoscyamus albus.
abstract: BACKGROUND
  Hyoscyamus albus L. (Solanaceae) an old medicinal plant is a rich source of tropane and nortropane alkaloids which confers to this plant a number of very interesting and beneficial therapeutic effects.
  
  
  OBJECTIVE
  Calystegines that are polyhydroxylated alkaloids and imino-sugars poccess significant glycosidases inhibitory activities and are therefore good candidats for the treatment of diabetes mellitus.
  
  
  METHODS
  Calystegines extracted from Hyoscyamys albus seeds were tested for teir acute oral toxicity and investigated for their in-vivo antidiabetic effect on Streptozotocine induced diabetes in mice.
  
  
  METHODS
  Calystegines were extracted from the seeds plant using an Ion exchange column; the remaining extract was then administrated orally to mice at several single doses for acute toxicity assay. A dose of 130mg/kg streptozotocine was injected to mice to induce diabetes mellitus, and diabetic mice were treated orally during 20days with 10mg/kg and 20mg/kg calystegines and 20mg/kg glibenclamide as the reference drug.
  
  
  RESULTS
  Acute oral toxicity showed that calystegines are not toxic up to a dose of 2000mg/kg with absence of any signs of intoxication and damages in Liver and kidney tissues. The nortropane alkaloids markedly reduced blood glucose levels and lipid parameters of diabetic mice to normal concentrations after 20days of treatment at 10mg/kg and 20mg/kg (p<0.05). Histopathological study of diabetic mice pancreas indicated that calystegines of Hyoscyamus albus have minimized streptozotocine damages on β-cells of islets of langerhans, stimulated β-cells regeneration and improved with this insulin secretion.
  
  
  CONCLUSIONS
  The findings of this study suggest that calystegines are potent antidiabetic agents with antihyperglicemic and hypolipidemic effects, and a protective fonction on pancreas in streptozotocin induced diabetes in mice.
authors.affiliation: Laboratoire de Biotechnologie végétale et d'Ethnobotanique, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algérie. Electronic address: lynda06bourebaba@gmail.com.
authors.forename: Lynda
authors.initials: L
authors.lastname: Bourebaba
authors.affiliation: Laboratoire de Biotechnologie végétale et d'Ethnobotanique, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algérie.
authors.forename: Souaad
authors.initials: S
authors.lastname: Saci
authors.affiliation: Laboratoire de Biotechnologie végétale et d'Ethnobotanique, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algérie.
authors.forename: Damia
authors.initials: D
authors.lastname: Touguit
authors.affiliation: Laboratoire de Biotechnologie végétale et d'Ethnobotanique, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algérie.
authors.forename: Lynda
authors.initials: L
authors.lastname: Gali
authors.affiliation: Laboratoire de Biotechnologie végétale et d'Ethnobotanique, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algérie.
authors.forename: Schahinez
authors.initials: S
authors.lastname: Terkmane
authors.affiliation: Laboratoire de Biotechnologie végétale et d'Ethnobotanique, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algérie.
authors.forename: Naima
authors.initials: N
authors.lastname: Oukil
authors.affiliation: Laboratoire de Biotechnologie végétale et d'Ethnobotanique, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algérie.
authors.forename: Fatiha
authors.initials: F
authors.lastname: Bedjou
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications / Q000188:drug therapy*; D004195:Disease Models, Animal; D004327:Drinking Behavior / Q000187:drug effects; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D005951:Glucose Tolerance Test; D032304:Hyoscyamus / Q000737:chemistry*; D006943:Hyperglycemia / Q000097:blood / Q000150:complications / Q000188:drug therapy; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D010179:Pancreas / Q000187:drug effects / Q000473:pathology; D012639:Seeds / Q000737:chemistry; D013311:Streptozocin; D023382:Toxicity Tests, Acute; D014326:Tropanes / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D007004:Hypoglycemic Agents; D008055:Lipids; D014326:Tropanes; C055033:calystegin; D013311:Streptozocin
keywords: Calystegines; Diabetes; Hyoscyamus albus L.; Hyperglycemia; Streptozotocine; Toxicity