title: Transcriptome Analysis Reveals Candidate Genes involved in Blister Blight defense in Tea (Camellia sinensis (L) Kuntze).
abstract: To unravel the molecular mechanism of defense against blister blight (BB) disease caused by an obligate biotrophic fungus, Exobasidium vexans, transcriptome of BB interaction with resistance and susceptible tea genotypes was analysed through RNA-seq using Illumina GAIIx at four different stages during ~20-day disease cycle. Approximately 69 million high quality reads were assembled de novo, yielding 37,790 unique transcripts with more than 55% being functionally annotated. Differentially expressed, 149 defense related transcripts/genes, namely defense related enzymes, resistance genes, multidrug resistant transporters, transcription factors, retrotransposons, metacaspases and chaperons were observed in RG, suggesting their role in defending against BB. Being present in the major hub, putative master regulators among these candidates were identified from predetermined protein-protein interaction network of Arabidopsis thaliana. Further, confirmation of abundant expression of well-known RPM1, RPS2 and RPP13 in quantitative Real Time PCR indicates salicylic acid and jasmonic acid, possibly induce synthesis of antimicrobial compounds, required to overcome the virulence of E. vexans. Compendiously, the current study provides a comprehensive gene expression and insights into the molecular mechanism of tea defense against BB to serve as a resource for unravelling the possible regulatory mechanism of immunity against various biotic stresses in tea and other crops.
authors.affiliation: Biotechnology Department, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, 176061.
authors.forename: Kuldip
authors.initials: K
authors.lastname: Jayaswall
authors.affiliation: Biotechnology Department, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, 176061.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Mahajan
authors.affiliation: Biotechnology Department, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, 176061.
authors.forename: Gagandeep
authors.initials: G
authors.lastname: Singh
authors.affiliation: Biotechnology Department, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, 176061.
authors.forename: Rajni
authors.initials: R
authors.lastname: Parmar
authors.affiliation: Biotechnology Department, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, 176061.
authors.forename: Romit
authors.initials: R
authors.lastname: Seth
authors.affiliation: Biotechnology Department, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, 176061.
authors.forename: Aparnashree
authors.initials: A
authors.lastname: Raina
authors.affiliation: Biotechnology Department, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, 176061.
authors.forename: Mohit Kumar
authors.initials: MK
authors.lastname: Swarnkar
authors.affiliation: Biotechnology Department, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, 176061.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Biotechnology Department, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, 176061.
authors.forename: Ravi
authors.initials: R
authors.lastname: Shankar
authors.affiliation: Biotechnology Department, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, 176061.
authors.forename: Ram Kumar
authors.initials: RK
authors.lastname: Sharma
mesh_terms: D028241:Camellia sinensis / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D019295:Computational Biology / Q000379:methods; D060467:Disease Resistance / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions / Q000235:genetics; D058977:Molecular Sequence Annotation; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D059467:Transcriptome