title: Experimental design-based optimization of lipid nanocarrier as delivery system against Mycobacterium species: in vitro and in vivo evaluation.
abstract: The study aimed to optimize self-nanoemulsifying drug delivery system using experimental design using excipients holding innate anti-mycobacterium activities followed with characterizations for responses such as optical clarity (Y1), zone of inhibition (ZOI) against Mycobacterium smegmatis strains (Y2, Y3), and globular size (Y4). The optimized formulations (OF1-OF3) were further characterized for responses and evaluated for zeta potential, minimum inhibition concentration (MIC) against non-pathogenic and tubercular strains, morphological (electron microscopy and atomic force microscopy), and confocal laser scanning microscopy (CLSM) studies. The desirability analysis suggested that the predicted values of the OF1 for the responses Y1, Y2, Y3, and Y4were 0.137, 22.77 mm, 21.9 mm, and 191.11 nm, respectively. The morphological assessment confirmed the in vitro studies and established the inhibition mechanism as evidenced with oozing, ablation, and cell-wall fragmentation followed with cell disruption. The OF1, OF2, and OF3 showed an MIC value at 8.8 ± 0.56 mg/ml, 12.5 ± 0.22 mg/ml, and 15.0 ± 0.4 mg/ml, respectively, corroborating effectiveness against tubercular strain. CLSM studies revealed 75.1, 80.3, and 88.7% as an intense fluorescence intensity of OF1, OF2, and OF3, respectively, as compared with dye solution (∼53%). Conclusively, it can be inferred that the delivery of anti-tubercular drugs might be reassessed using excipients with inherent anti-mycobacterium activities.
authors.affiliation: a Department of Pharmaceutical Sciences and Technology , Birla Institute of Technology , Mesra, Ranchi , Jharkhand , India.
authors.forename: Afzal
authors.initials: A
authors.lastname: Hussain
authors.affiliation: a Department of Pharmaceutical Sciences and Technology , Birla Institute of Technology , Mesra, Ranchi , Jharkhand , India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar Singh
authors.affiliation: a Department of Pharmaceutical Sciences and Technology , Birla Institute of Technology , Mesra, Ranchi , Jharkhand , India.
authors.forename: Priya
authors.initials: P
authors.lastname: Ranjan Prasad Verma
authors.affiliation: b Department of Biomedical Lab Technology, Birla Institute of Technology , University Polytechnic , Mesra, Ranchi , Jharkhand , India.
authors.forename: Neeru
authors.initials: N
authors.lastname: Singh
authors.affiliation: c Department of Pharmaceutics , Jamia Hamdard (Hamdard University) , New Delhi , India.
authors.forename: Farhan
authors.initials: F
authors.lastname: Jalees Ahmad
mesh_terms: D000890:Anti-Infective Agents; D016503:Drug Delivery Systems; D008055:Lipids; D009161:Mycobacterium; D053758:Nanoparticles; D012107:Research Design
chemical_list: D000890:Anti-Infective Agents; D008055:Lipids
keywords: Mycobacterium species; electron microscopy studies; SNEDDS formulations; anti-tubercular study; ex vivo CLSM