title: Protective Effects of Soy Oligopeptides in Ultraviolet B-Induced Acute Photodamage of Human Skin.
abstract: Aim. We explored the effects of soy oligopeptides (SOP) in ultraviolet B- (UVB-) induced acute photodamage of human skin in vivo and foreskin ex vivo. Methods. We irradiated the forearm with 1.5 minimal erythemal dose (MED) of UVB for 3 consecutive days, establishing acute photodamage of skin, and topically applied SOP. Erythema index (EI), melanin index, stratum corneum hydration, and transepidermal water loss were measured by using Multiprobe Adapter 9 device. We irradiated foreskin ex vivo with the same dose of UVB (180 mJ/cm(2)) for 3 consecutive days and topically applied SOP. Sunburn cells were detected by using hematoxylin and eosin staining. Apoptotic cells were detected by using terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Cyclobutane pyrimidine dimers (CPDs), p53 protein, Bax protein, and Bcl-2 protein were detected by using immunohistochemical staining. Results. Compared with UVB group, UVB-irradiated skin with topically applied SOP showed significantly decreased EI. Compared with UVB group, topical SOP significantly increased Bcl-2 protein expression and decreased CPDs-positive cells, sunburn cells, apoptotic cells, p53 protein expression, and Bax protein expressions in the epidermis of UVB-irradiated foreskin. Conclusion. Our study demonstrated that topical SOP can protect human skin against UVB-induced photodamage.
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Bing-Rong
authors.identifier: 0000-0002-7965-7624
authors.initials: BR
authors.lastname: Zhou
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Ma
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jia-An
authors.initials: JA
authors.lastname: Zhang
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Felicia
authors.initials: F
authors.lastname: Permatasari
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Dan
authors.identifier: 0000-0001-6074-1298
authors.initials: D
authors.lastname: Luo
mesh_terms: D000287:Administration, Topical; D000328:Adult; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D004817:Epidermis / Q000187:drug effects / Q000528:radiation effects; D004890:Erythema / Q000473:pathology; D006801:Humans; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D011740:Pyrimidine Dimers / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000528:radiation effects*; D030262:Soybean Proteins / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014466:Ultraviolet Rays; D014870:Water Loss, Insensible / Q000187:drug effects / Q000528:radiation effects; D055815:Young Adult; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D009842:Oligopeptides; D020011:Protective Agents; D011740:Pyrimidine Dimers; D030262:Soybean Proteins; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein