title: Repair of traumatized mammalian hair cells via sea anemone repair proteins.
abstract: Mammalian hair cells possess only a limited ability to repair damage after trauma. In contrast, sea anemones show a marked capability to repair damaged hair bundles by means of secreted repair proteins (RPs). Previously, it was found that recovery of traumatized hair cells in blind cavefish was enhanced by anemone-derived RPs; therefore, the ability of anemone RPs to assist recovery of damaged hair cells in mammals was tested here. After a 1 h incubation in RP-enriched culture media, uptake of FM1-43 by experimentally traumatized murine cochlear hair cells was restored to levels comparable to those exhibited by healthy controls. In addition, RP-treated explants had significantly more normally structured hair bundles than time-matched traumatized control explants. Collectively, these results indicate that anemone-derived RPs assist in restoring normal function and structure of experimentally traumatized hair cells of the mouse cochlea.
authors.affiliation: Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70503, USA.
authors.forename: Pei-Ciao
authors.initials: PC
authors.lastname: Tang
authors.affiliation: Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70503, USA.
authors.forename: Karen Müller
authors.initials: KM
authors.lastname: Smith
authors.affiliation: Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70503, USA gmw@louisiana.edu.
authors.forename: Glen M
authors.identifier: 0000-0003-3531-1231
authors.initials: GM
authors.lastname: Watson
mesh_terms: D000818:Animals; D003470:Culture Media / Q000494:pharmacology; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects / Q000473:pathology*; D051379:Mice; D011506:Proteins / Q000494:pharmacology*; D020543:Proteome / Q000378:metabolism; D011726:Pyridinium Compounds / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012615:Sea Anemones / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D003470:Culture Media; C073804:FM1 43; D011506:Proteins; D020543:Proteome; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds
keywords: Cochlea; Deafness; Hair cell evolution; Mouse; Outer hair cells