title: In vitro bioactivity studies of ceramic structures isolated from marine sponges.
abstract: In this work, we focused on the potential of bioceramics from different marine sponges-namely Petrosia ficiformis, Agelas oroides and Chondrosia reniformis-for novel biomedical/industrial applications. The bioceramics from these sponges were obtained after calcination at 750 °C for 6 h in a furnace. The morphological characteristics were evaluated by scanning electron microscopy (SEM). The in vitro bioactivity of the bioceramics was evaluated in simulated body fluid (SBF) after 14 and 21 d. Observation of the bioceramics by SEM after immersion in SBF solution, coupled with spectroscopic elemental analysis (EDS), showed that the surface morphology was consistent with a calcium-phosphate (Ca/P) coating, similar to hydroxyapatite crystals (HA). Evaluation of the characteristic peaks of Ca/P crystals by Fourier transform infrared spectroscopy and x-ray diffraction further confirmed the existence of HA. Cytotoxicity studies were carried out with the different ceramics and these were compared with a commercially available Bioglass(®). In vitro tests demonstrated that marine bioceramics from these sponges are non-cytotoxic and have the potential to be used as substitutes for synthetic Bioglass(®).
authors.affiliation: 3Bs Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco GMR-Portugal, Portugal. ICVS/3Bs-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Barros
authors.forename: Ivo M
authors.initials: IM
authors.lastname: Aroso
authors.forename: Tiago H
authors.initials: TH
authors.lastname: Silva
authors.forename: João F
authors.initials: JF
authors.lastname: Mano
authors.forename: Ana Rita C
authors.initials: AR
authors.lastname: Duarte
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D002516:Ceramics / Q000737:chemistry* / Q000302:isolation & purification*; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D011161:Porifera / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C020243:calcium phosphate