title: Membrane-bound electron transport systems of an anammox bacterium: A complexome analysis.
abstract: Electron transport, or oxidative phosphorylation, is one of the hallmarks of life. To this end, prokaryotes evolved a vast variety of protein complexes, only a small part of which have been discovered and studied. These protein complexes allow them to occupy virtually every ecological niche on Earth. Here, we applied the method of proteomics-based complexome profiling to get a better understanding of the electron transport systems of the anaerobic ammonium-oxidizing (anammox) bacteria, the N2-producing key players of the global nitrogen cycle. By this method nearly all respiratory complexes that were previously predicted from genome analysis to be involved in energy and cell carbon fixation were validated. More importantly, new and unexpected ones were discovered. We believe that complexome profiling in concert with (meta)genomics offers great opportunities to expand our knowledge on bacterial respiratory processes at a rapid and massive pace, in particular in new and thus far poorly investigated non-model and environmentally-relevant species.
authors.affiliation: Department of Microbiology, Institute of Water and Wetland Research, Radboud University Nijmegen, 6525 AJ Nijmegen, The Netherlands. Electronic address: almeida@science.ru.nl.
authors.forename: Naomi M
authors.initials: NM
authors.lastname: de Almeida
authors.affiliation: Nijmegen Center for Mitochondrial Disorders, Radboud Proteomics Center, Translational Metabolic Laboratory, Department of Laboratory Medicine, Radboudumc, Geert Grooteplein-Zuid 10, 6525 GA Nijmegen, The Netherlands.
authors.forename: Hans J C T
authors.initials: HJ
authors.lastname: Wessels
authors.affiliation: Department of Microbiology, Institute of Water and Wetland Research, Radboud University Nijmegen, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Rob M
authors.initials: RM
authors.lastname: de Graaf
authors.affiliation: Department of Microbiology, Institute of Water and Wetland Research, Radboud University Nijmegen, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Christina
authors.initials: C
authors.lastname: Ferousi
authors.affiliation: Department of Microbiology, Institute of Water and Wetland Research, Radboud University Nijmegen, 6525 AJ Nijmegen, The Netherlands; Kluyver Laboratory for Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands.
authors.forename: Mike S M
authors.initials: MS
authors.lastname: Jetten
authors.affiliation: Department of Microbiology, Institute of Water and Wetland Research, Radboud University Nijmegen, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Jan T
authors.initials: JT
authors.lastname: Keltjens
authors.affiliation: Department of Microbiology, Institute of Water and Wetland Research, Radboud University Nijmegen, 6525 AJ Nijmegen, The Netherlands. Electronic address: bkartal@mpi-bremen.de.
authors.forename: Boran
authors.initials: B
authors.lastname: Kartal
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism*; D000693:Anaerobiosis / Q000502:physiology*; D001419:Bacteria / Q000378:metabolism*; D001669:Biochemical Phenomena / Q000502:physiology; D019069:Cell Respiration / Q000502:physiology; D004579:Electron Transport / Q000502:physiology*; D004583:Electrons; D008566:Membranes / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D010766:Phosphorylation / Q000502:physiology; D040901:Proteomics / Q000379:methods; D000644:Quaternary Ammonium Compounds / Q000378:metabolism
chemical_list: D064751:Ammonium Compounds; D000644:Quaternary Ammonium Compounds; D009584:Nitrogen
keywords: Anaerobic ammonium oxidation (anammox); Blue Native gel electrophoresis; Complexome profiling; Respiratory complexes