title: Enzyme-mediated polymerization inside engineered protein cages.
abstract: Engineered variants of the capsid-forming enzyme lumazine synthase, AaLS, were used as nanoreactors for an enzyme-mediated polymerization. Oxidation of 3,3-diaminobenzidine (DAB) by the engineered ascorbate peroxidase APEX2 encapsulated in AaLS capsids resulted in templated formation of polyDAB-capsid nanoparticles of homogeneous size and shape.
authors.affiliation: Laboratory of Organic Chemistry, ETH Zürich, 8093 Zürich, Switzerland. hilvert@org.chem.ethz.ch.
authors.forename: Raphael
authors.initials: R
authors.lastname: Frey
authors.affiliation: Laboratory of Organic Chemistry, ETH Zürich, 8093 Zürich, Switzerland. hilvert@org.chem.ethz.ch.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Laboratory of Organic Chemistry, ETH Zürich, 8093 Zürich, Switzerland. hilvert@org.chem.ethz.ch.
authors.forename: Donald
authors.initials: D
authors.lastname: Hilvert
mesh_terms: D015100:3,3'-Diaminobenzidine / Q000031:analogs & derivatives / Q000737:chemistry; D060387:Ascorbate Peroxidases / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry; D009097:Multienzyme Complexes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis*; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000737:chemistry
chemical_list: D009097:Multienzyme Complexes; D011108:Polymers; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D015100:3,3'-Diaminobenzidine; C044822:6,7-dimethyl-8-ribityllumazine synthase; D060387:Ascorbate Peroxidases