title: Diagnostic value of microRNAs as biomarkers for cholangiocarcinoma.
abstract: BACKGROUND
  It has been reported that microRNAs might serve as biomarkers for cholangiocarcinoma (CCA) detection, but their diagnostic accuracy are unclear and controversial.
  
  
  OBJECTIVE
  To evaluate the diagnostic accuracy of microRNAs for CCA.
  
  
  METHODS
  PubMed, Embase, Web of Science and Cochrane Library were systematically searched to identify relevant articles by using the key words "cholangiocarcinoma" and "microRNA". The methodological quality of each study was assessed by QUADAS-2. According to the inclusive and exclusive criteria, 11 articles were identified and analyzed by Meta-disc software v.1.4 and STATA 12.0 software package.
  
  
  RESULTS
  11 articles with 430 CCA patients and 406 controls were identified. The results showed that the pooled sensitivity was 0.756 (95% CI: 0.693-0.810), specificity was 0.914 (95% CI: 0.861-0.947), positive likelihood ratio was 8.747 (95% CI: 5.320-14.381), negative likelihood ratio was 0.267 (95% CI: 0.208-0.341) and diagnostic odds ratio was 32.791 (95% CI: 17.327, 62.060). In addition, the area under the summary ROC cure (AUC) was 0.90 (95% CI: 0.87-0.93).
  
  
  CONCLUSIONS
  MicroRNAs have great potential as biomarkers for the diagnosis of CCA patients. However, the clinical application of microRNA assays for CCA diagnosis still needs further validation by more prospective studies.
authors.affiliation: Dept. Epidemiology and Statistics, School of Public Health, Jilin University, Changchun, Jilin, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Dept. Epidemiology and Statistics, School of Public Health, Jilin University, Changchun, Jilin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Dept. Epidemiology and Statistics, School of Public Health, Jilin University, Changchun, Jilin, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Mathematics and Statistics, Northeast Normal University, Changchun, Jilin, China. Electronic address: liuxin2015722010@163.com.
authors.forename: Chunpeng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Dept. Nuclear Medicine, 2nd Hospital, Jilin University, Changchun, China. Electronic address: 1370118262@qq.com.
authors.forename: Yinlong
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D001650:Bile Duct Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics; D018281:Cholangiocarcinoma / Q000175:diagnosis* / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D016017:Odds Ratio; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Biomarker; Cholangiocarcinoma; Meta-analysis; MicroRNAs