title: Gene regulation with carbon-based siRNA conjugates for cancer therapy.
abstract: We report fluorescent carbon nanoparticle (FCN)-based small interfering RNA (siRNA) conjugates (C-siRNA) for gene regulation and cancer therapy. The C-siRNA has a core of chitosan-derived FCN and a shell of siRNA, and can down-regulate the expression of polo-like kinase-1 (Plk1), a master regulator of mitosis, via siRNA targeting Plk1 (siPlk1), for cancer therapy. The required amount of the FCNs is only ∼1/30 of that of the gold nanoparticles in delivering equal amount of siRNA. The C-siPlk1 led to ∼80% knockdown of cellular Plk1 mRNA in A375 cells, and induced apoptosis of the A375 cells (31.9%) and MCF-7 cells (20.33%), much higher than those by commercial nonviral gene delivery vectors, such as Lipofectamine 2000 in both cell lines (apoptosis rate < 10%). After the C-siPlk1 was administrated to A375 tumor-bearing mice intravenously, the tumor volume was less than 1/11 of the control groups. The C-siRNA can thus be powerful tools for gene delivery and gene therapy.
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, ZhongGuanCun BeiYiTiao, Beijing, 100190, China.
authors.forename: Lingmin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, ZhongGuanCun BeiYiTiao, Beijing, 100190, China.
authors.forename: Wenfu
authors.initials: W
authors.lastname: Zheng
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, ZhongGuanCun BeiYiTiao, Beijing, 100190, China.
authors.forename: Rongbing
authors.initials: R
authors.lastname: Tang
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, ZhongGuanCun BeiYiTiao, Beijing, 100190, China.
authors.forename: Nuoxin
authors.initials: N
authors.lastname: Wang
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, ZhongGuanCun BeiYiTiao, Beijing, 100190, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, ZhongGuanCun BeiYiTiao, Beijing, 100190, China. Electronic address: xingyujiang@nanoctr.cn.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D005786:Gene Expression Regulation / Q000235:genetics; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D061986:MCF-7 Cells; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry; D058726:Nanoconjugates / Q000008:administration & dosage*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009374:Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D016896:Treatment Outcome
chemical_list: D053769:Nanocapsules; D058726:Nanoconjugates; D037742:Nanotubes, Carbon; D034741:RNA, Small Interfering
keywords: Cancer therapy; Fluorescent carbon nanoparticle; Gene regulation; Plk1 gene; Small interfering RNA