title: Isolation and structure elucidation of pectic polysaccharide from rose hip fruits (Rosa canina L.).
abstract: A pectic polysaccharide from rose hip (RH) fruits has been obtained by extraction with 1% aqueous citric acid. It was found that the polysaccharide fraction mainly consisted of galacturonic acid (45.5%) next to galactose (5.5%) and arabinose (4.7%). RH pectin is having a relatively high degree of methylesterification (62%) and acetylation (10%) and consists of different molecular weight populations in the range of 10-100kDa. Enzymatic fingerprinting was performed using a combination of pectin lyase (PL) and endo-polygalacturonase. Detailed information about the structure and level of galacturonic acid oligomers released was obtained using LC-HILIC-MS/ELSD and HPAEC. Predominantly, unsaturated and methyl-esterified oligomers (DP 3-5) were released indicating that high proportions of methylesterified 'PL degradable' areas were present within the pectin. The data revealed that homogalacturonan is the main building block of the extracted pectin and consists of long methylesterified/acetylated GalA sequences interspersed with small blocks of non-methyl-esterified GalA units.
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Laboratory of Biologically Active Substances, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria. Electronic address: mogn@orgchm.bas.bg.
authors.forename: Manol
authors.initials: M
authors.lastname: Ognyanov
authors.affiliation: Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands. Electronic address: connieremoroza@hotmail.com.
authors.forename: Connie
authors.initials: C
authors.lastname: Remoroza
authors.affiliation: Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands. Electronic address: henk.schols@wur.nl.
authors.forename: Henk A
authors.initials: HA
authors.lastname: Schols
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Laboratory of Biologically Active Substances, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria. Electronic address: yordan_georgiev88@abv.bg.
authors.forename: Yordan
authors.initials: Y
authors.lastname: Georgiev
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Laboratory of Biologically Active Substances, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria; Innovative Technological Centre Ltd., 20 Dr. G. M. Dimitrov str., 4000 Plovdiv, Bulgaria. Electronic address: lbas@plov.omega.bg.
authors.forename: Maria
authors.initials: M
authors.lastname: Kratchanova
authors.affiliation: Innovative Technological Centre Ltd., 20 Dr. G. M. Dimitrov str., 4000 Plovdiv, Bulgaria. Electronic address: info@itc-plovdiv.com.
authors.forename: Christo
authors.initials: C
authors.lastname: Kratchanov
mesh_terms: D000107:Acetylation; D004951:Esterification; D005638:Fruit / Q000737:chemistry*; D006868:Hydrolysis; D008970:Molecular Weight; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism; D058105:Polymerization; D011133:Polysaccharide-Lyases / Q000378:metabolism; D027826:Rosa / Q000737:chemistry*
chemical_list: D010368:Pectins; D011096:Polygalacturonase; D011133:Polysaccharide-Lyases; C027349:pectin lyase
keywords: HILIC-MS/ELSD; Pectic polysaccharides; Pectin lyase; Rose hip; endo-Polygalacturonase II