title: Impact of the Action Time of Inducers on Differentiation of 3T3-L1 Cells.
abstract: Objective To explore the effect of the action time of inducers on the differentiation of 3T3-L1 cells to adipocytes. Methods According to the "Cocktail" method,3T3-L1 cells were divided into three groups according to the action time of inducers,with the action time being 2,3 or 4 days,respectively. Cell morphology was observed using inverted microscope and adipose content were detected by Oil red "O" staining and detection of triglyceride. The cell viability was identified by trypan blue staining method. Results The proportion of samples (n=12) with differentiation rate above 80% in group A was 66% (12/18),while the differentiation rate of all the samples (n=18)in group B and group C were above 80%. For the Oil red "O",the OD value at 510 nm in group C was 2.59±0.17,which was significantly higher than that in group A (2.12±0.47;F=6.62,P=0.0001)and group B (2.20±0.17;F=5.15,P=0.0001),while no significant difference was found between group A and group B (F=1.14,P=0.74). As for the triglyceride,the value in group C was (1351.04±119.01)ng/ml,which was significantly higher than that in group A[ (1077.88±272.75)ng/ml;F=6.73,P=0.001] and group B [(1089.38±115.39)ng/ml;F=5.78,P=0.001],while no difference was found between group A and group B (F=0.27,P=0.64). The cell viability in group A,B,and C was (98.3±1.2)%,(98.5±1.8)%,and (98.9±2.1)%,respectively,showing no significant difference (F=0.18,P=0.83). Conclusions The modified procedure for the differentiation of 3T3-L1 cells to adipocytes can increase the differentiation rate and thus may be applied for establishing adipocyte models. The recommended action time is three days.
authors.affiliation: Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
authors.forename: Xin-nong
authors.initials: XN
authors.lastname: Liu
authors.affiliation: Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
authors.forename: Xiu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
authors.forename: Tian-jia
authors.initials: TJ
authors.lastname: Li
authors.affiliation: Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
authors.forename: Zhan-qi
authors.initials: ZQ
authors.lastname: Wang
authors.affiliation: Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
authors.forename: Leng
authors.initials: L
authors.lastname: Ni
authors.affiliation: Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
authors.forename: Bao
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
authors.forename: Chang-wei
authors.initials: CW
authors.lastname: Liu
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D051379:Mice; D013997:Time Factors