title: Correlations between gastric cancer family history and ROBO2 and RASSF2A gene methylations.
abstract: OBJECTIVE
  To explore the correlation between ROBO2 and RASSF2A gene methylations and gastric cancer family history.
  
  
  METHODS
  ROBO2 and RASSF2A gene methylations in gastric cancer tissues and peri.cancerous tissues were detected with methylation.specific PCR in 36. patients with gastric cancer family history and 33 without gastric cancer family history. The correlations of ROBO2 and RASSF2A gene methylations with family history, and clinical and pathological characteristics were analyzed.
  
  
  RESULTS
  ROBO2 and RASSF2A gene methylations were all significantly higher in gastric cancer tissues (30% and 26%) than in peri-cancerous tissues (0% and 0%) (all P < 0.05). ROBO2 gene methylation was significantly lower in the patients with gastric cancer family history (17%, 6/36) than in the patients without gastric cancer family history (41%, 15/33) (P < 0.05).
  
  
  CONCLUSIONS
  ROBO2 and RASSF2A gene methylations may be related to gastric tumorigenesis, and ROBO2 gene methylation is associated with sporadic gastric cancer.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Chang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Yan-Ru
authors.initials: YR
authors.lastname: Qin
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Pathology, Linzhou Center Hospital, Linzhou, China.
authors.forename: Hai-Yun
authors.initials: HY
authors.lastname: Guo
authors.affiliation: Department of Pathology, Linzhou Center Hospital, Linzhou, China.
authors.forename: Qing-Li
authors.initials: QL
authors.lastname: Zhu
mesh_terms: D000328:Adult; D000368:Aged; D019175:DNA Methylation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011971:Receptors, Immunologic / Q000235:genetics*; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D000097905:Roundabout Proteins
chemical_list: D011971:Receptors, Immunologic; D025521:Tumor Suppressor Proteins; C425300:RASSF2 protein, human; C498890:ROBO2 protein, human; D000097905:Roundabout Proteins