title: Production of starch nanoparticles using normal maize starch via heat-moisture treatment under mildly acidic conditions and homogenization.
abstract: Normal maize starch was subjected to heat-moisture treatment (HMT) under mildly acidic conditions (0.000, 0.050, or 0.075M H2SO4) for various treatment times (3, 5, or 8h) followed by homogenization up to 60min to prepare nanoparticles. The combination of HMT (0.075M, for 8h) and homogenization (60min) produced nanoparticles with diameters of less than 50nm at a yield higher than 80%. X-ray diffractometry and size-exclusion chromatography revealed that HMT under mildly acidic conditions selectively hydrolyzed the starch chains (especially amylose and/or long chains of amylopectin) in the amorphous region of the granules without significant damage to the crystalline structure, however, modification of the molecular structure in the amorphous region increased fragility of the granules during homogenization. Homogenization for 60min caused obvious damage in the long-range crystalline structure of the HMT starch (0.15N, for 8h), while the short-range chain associations (FT-IR) remained intact.
authors.affiliation: Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, South Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, South Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, South Korea; Present address: Applied Product Development Team, National Development Institute of Korean Medicine, Gyeongsan 38540, South Korea.
authors.forename: MyoungLae
authors.initials: M
authors.lastname: Cho
authors.affiliation: College of Liberal Arts, Shinhan University, Uijeongbu-Si 11644, South Korea.
authors.forename: Ju Hun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, South Korea. Electronic address: jongkim@kangwon.ac.kr.
authors.forename: Jong-Yea
authors.initials: JY
authors.lastname: Kim
mesh_terms: D006358:Hot Temperature; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D013213:Starch; D014867:Water / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry
chemical_list: D014867:Water; D013213:Starch
keywords: HMT under acidic conditions; Homogenization; Nanoparticles; Normal maize starch; Yield