title: Adipokines induce pro-inflammatory factors in activated Cd4+ T cells from osteoarthritis patient.
abstract: Osteoarthritis (OA) is a chronic systemic musculoskeletal disorder involving inflammation, immunity, and metabolic alterations. OA is commonly regarded as non-inflammatory disease; still inflammation is recognized as contributing to the symptoms and progression of OA. New evidence suggests that adipokines are involved in the pathophysiology of OA and might modulate the production of inflammatory mediators including in immune cells. However, the role of immune component in osteoarthritis is still poorly investigated. To gain further insights into the interaction of immune cells in OA and the role of adipokines on these cells, we performed experiments aimed to determine the cytokine profile in activated CD4+T cells from OA patients. For completeness, we also explored the cross talk between T lymphocytes and chondrocytes in OA by co-culturing human primary chondrocytes with activated CD4+T cells in two ways: the first by incubating the cells by direct contact (D.C.) or by transwell system. Our results show that the exposure of activated CD4+T cells to adipokines modulates IL-6, IL-8, and CCL-3 production. In addition, the production of key macromolecules of ECM (aggrecan and collagen-2) and matrix metalloproteinase 13 (MMP-13) in co-cultured chondrocytes with activated CD4+T cells was altered. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1299-1303, 2017.
authors.affiliation: SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigación Sanitaria de Santiago), Research Laboratory 9 (NEIRID LAB: Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Santiago University Clinical Hospital, Building C, Level-2, Door 9, Santiago de Compostela 15706, Spain.
authors.forename: Morena
authors.initials: M
authors.lastname: Scotece
authors.affiliation: SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigación Sanitaria de Santiago), Research Laboratory 9 (NEIRID LAB: Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Santiago University Clinical Hospital, Building C, Level-2, Door 9, Santiago de Compostela 15706, Spain.
authors.forename: Tamara
authors.initials: T
authors.lastname: Pérez
authors.affiliation: SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigación Sanitaria de Santiago), Research Laboratory 9 (NEIRID LAB: Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Santiago University Clinical Hospital, Building C, Level-2, Door 9, Santiago de Compostela 15706, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Conde
authors.affiliation: SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigación Sanitaria de Santiago), Research Laboratory 9 (NEIRID LAB: Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Santiago University Clinical Hospital, Building C, Level-2, Door 9, Santiago de Compostela 15706, Spain.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Abella
authors.affiliation: SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigación Sanitaria de Santiago), Research Laboratory 9 (NEIRID LAB: Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Santiago University Clinical Hospital, Building C, Level-2, Door 9, Santiago de Compostela 15706, Spain.
authors.forename: Veronica
authors.initials: V
authors.lastname: López
authors.affiliation: SERGAS-IDIS, Division of Orthopaedic Surgery and Traumatology, Santiago University Clinical Hospital, Santiago de Compostela, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Pino
authors.affiliation: Epidemiology, Genetics and Atherosclerosis Research Group on Systemic Inflammatory Diseases, Universidad de Cantabria and IDIVAL, Santander, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Gonzalez-Gay
authors.affiliation: SERGAS, Division of Rheumatology, Department of Medicine, Santiago University Clinical Hospital, University of Santiago, Santiago de Compostela, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Gomez-Reino
authors.affiliation: SERGAS, Division of Rheumatology, Department of Medicine, Santiago University Clinical Hospital, University of Santiago, Santiago de Compostela, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Mera
authors.affiliation: SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigación Sanitaria de Santiago), Research Laboratory 9 (NEIRID LAB: Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Santiago University Clinical Hospital, Building C, Level-2, Door 9, Santiago de Compostela 15706, Spain.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Gomez
authors.affiliation: SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigación Sanitaria de Santiago), Research Laboratory 9 (NEIRID LAB: Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Santiago University Clinical Hospital, Building C, Level-2, Door 9, Santiago de Compostela 15706, Spain.
authors.forename: Oreste
authors.initials: O
authors.lastname: Gualillo
mesh_terms: D052242:Adiponectin / Q000502:physiology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D016022:Case-Control Studies; D019902:Chondrocytes / Q000378:metabolism; D018920:Coculture Techniques; D006801:Humans; D020738:Leptin / Q000502:physiology*; D000071068:Lipocalin-2 / Q000502:physiology*; D010003:Osteoarthritis / Q000276:immunology*; D061251:Primary Cell Culture
chemical_list: D052242:Adiponectin; C489506:LCN2 protein, human; D020738:Leptin; D000071068:Lipocalin-2
keywords: adipokines; chondrocytes; immunity; inflammation; lymphocytes; osteoarthritis