title: Comparative study on the antioxidant capacities of synthetic influenza inhibitors and ellagic acid in model systems.
abstract: This study compares the antioxidant capacities in vitro of several synthetic and natural compounds applied and researched for influenza treatment - oseltamivir, isoprinosine, ellagic acid, vitamin E and vitamin C. Three chemical systems are utilized for the generation of reactive oxygen species (ROS) at pH 7.4 and pH 8.5: (1) Fenton's (Fe2++H2O2) for OH and -OH species (2) H2O2(3) NADH-phenazinemethosulfat, for superoxide radicals (O2-). The kinetics was evaluated by lucigenin-enhanced chemiluminescence. The calculated constants of inhibition k7describe the antioxidant capacity at the moment of oxidative burst. Their values do not necessarily correspond to the calculated total antioxidant activity. The obtained results revealed that the synthetic anti-influenza drugs (oseltamivir and isoprinosine) as well as ellagic acid possess pronounced scavenging properties mostly against superoxide radicals, comparable and higher than that of traditional natural antioxidants. Quantitative analysis of the antioxidant effects of the examined synthetic substances was performed. The results compared the corresponding effect of the average physiological concentrations and the applied therapeutic antioxidant dose. With these experiments we registered new aspects of their therapeutic activities, due to antioxidant properties against hydroxyl, superoxide radicals and H2O2oxidation.
authors.affiliation: Biophysics & Medical Physics, Sofia University "St. Kliment Ohridski", 5 James Boucher Blvd., 1164 Sofia, Bulgaria. Electronic address: elli_pavlova@abv.bg.
authors.forename: Elitsa L
authors.initials: EL
authors.lastname: Pavlova
authors.affiliation: Biophysics & Medical Physics, Sofia University "St. Kliment Ohridski", 5 James Boucher Blvd., 1164 Sofia, Bulgaria.
authors.forename: Nikolay N
authors.initials: NN
authors.lastname: Zografov
authors.affiliation: Department of Virology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Georgi Bonchev Str., 1113 Sofia, Bulgaria.
authors.forename: Lora S
authors.initials: LS
authors.lastname: Simeonova
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D004610:Ellagic Acid / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism; D007542:Inosine Pranobex / Q000494:pharmacology; D007501:Iron / Q000737:chemistry; D007700:Kinetics; D008163:Luminescent Measurements; D008954:Models, Biological; D009243:NAD / Q000378:metabolism; D053139:Oseltamivir / Q000494:pharmacology; D010084:Oxidation-Reduction; D012015:Reference Standards; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D000998:Antiviral Agents; C045076:Fenton's reagent; D009243:NAD; D014810:Vitamin E; D004610:Ellagic Acid; D053139:Oseltamivir; D006861:Hydrogen Peroxide; D007501:Iron; D001205:Ascorbic Acid; D007542:Inosine Pranobex
keywords: Constant of inhibition; Ellagic acid; Ellagic acid (PubChem CID 5281855); Isoprinosine; Isoprinosine (PubChem CID 37510); Luminescence; Oseltamivir; Oseltamivir (PubChem CID 65028); Vitamin C; Vitamin C (PubChem CID 54670067); Vitamin E; Vitamin E (PubChem CID 20353)