title: [MicroRNA-205 targeted Krüppel-like factor 12 and regulated MDA-MB-468 cells apoptosis in basal-like breast carcinoma].
abstract: OBJECTIVE
  To identify basal-like breast carcinoma (BLBC)-specific microRNA (miRs) and its target gene and to investigate the distinct biological function.
  
  
  METHODS
  Krüppel-like factor 12 (KLF12) 3'-UTR was constructed and luciferase reporter assay was performed for target gene. Expression levels of miR including its target genes were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot and immunohistochemistry (IHC). Further functional analyses were conducted which included apoptosis.
  
  
  RESULTS
  Luciferase assay showed miR-205 directly targets KLF12 through binding its 3'-UTR (P=0.001 6). QRT-PCR revealed that miR-205 expression levels were exclusively lower in MDA-MB-468 cell lines (P=0.00 7) and BLBC tumor tissues (P=0.007 4; n=3), but KLF12 were highly expressed in MDA-MB-468 cell lines (P=0.003 9); knock-up of miR-205 expression by transfection with its mimics in MDA-MB-468 cells substantially increased miR-205 expression level (P=0.000) and reduced KLF12 expression level (P=0.038). Western blot displayed that KLF12 expression levels were higher in tumor tissues when compared with normal breast tissues (P=0.008 3; n=9). Knock-up of miR-205 expression by transfection with its mimics promoted MDA-MB-468 cells apoptosis (P=0.006 1).
  
  
  CONCLUSIONS
  Our results suggest that miR-205 is a miR specific for BLBC which exerts tumor suppression role through negative regulated proto-oncogene KLF12 and promote cell apoptosis. MiR-205 and KLF12 may provide potential diagnosis molecular biomarker and possible new approach for the treatment for BLBC.
authors.affiliation: Department of Pathology, Jinshan Branch of Shanghai 6th People's Hospital, Shanghai 201599, Chian.
authors.forename: B
authors.initials: B
authors.lastname: Guan
authors.affiliation: Department of Pathology, Shanghai Pudong New Area People's Hospital, Shanghai 201299, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.forename: X H
authors.initials: XH
authors.lastname: Li
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhou
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis; D015153:Blotting, Western; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D051741:Kruppel-Like Transcription Factors; D035683:MicroRNAs; D000090063:Proto-Oncogene Mas; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D020413:3' Untranslated Regions; C436492:KLF12 protein, human; D051741:Kruppel-Like Transcription Factors; C000715470:MAS1 protein, human; D035683:MicroRNAs; D000090063:Proto-Oncogene Mas