title: Accelerated Stability Studies on Dried Extracts of Centella asiatica Through Chemical, HPLC, HPTLC, and Biological Activity Analyses.
abstract: Regulatory guidelines recommend systematic stability studies on a herbal product to establish its shelf life. In the present study, commercial extracts (Types I and II) and freshly prepared extract (Type III) of Centella asiatica were subjected to accelerated stability testing for 6 months. Control and stability samples were evaluated for organoleptics, pH, moisture, total phenolic content (TPC), asiatic acid, kaempherol, and high-performance thin layer chromatography fingerprints, and for antioxidant and acetylcholinesterase inhibitory activities. Markers and TPC and both the activities of each extract decreased in stability samples with respect to control. These losses were maximum in Type I extract and minimum in Type III extract. Higher stability of Type III extract than others might be attributed to the additional phytoconstituents and/or preservatives in it. Pearson correlation analysis of the results suggested that TPC, asiatic acid, and kaempferol can be taken as chemical markers to assess chemical and therapeutic shelf lives of herbal products containing Centella asiatica.
authors.affiliation: Punjabi University, Patiala, India.
authors.forename: Ishtdeep
authors.initials: I
authors.lastname: Kaur
authors.affiliation: Punjabi University, Patiala, India.
authors.forename: Nancy
authors.initials: N
authors.lastname: Suthar
authors.affiliation: Punjabi University, Patiala, India.
authors.forename: Jasmeen
authors.initials: J
authors.lastname: Kaur
authors.affiliation: Punjabi University, Patiala, India.
authors.forename: Yogita
authors.initials: Y
authors.lastname: Bansal
authors.affiliation: Punjabi University, Patiala, India gulshan@pbi.ac.in.
authors.forename: Gulshan
authors.initials: G
authors.lastname: Bansal
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D028041:Centella / Q000737:chemistry*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts
keywords: Centella asiatica; acetylcholinesterase; antioxidant; stability; total phenolic content