title: Screening for Antimicrobial Activity of Wood Rotting Higher Basidiomycetes Mushrooms from Uruguay against Phytopathogens.
abstract: In this work, the antimicrobial activity of extracts of wood rotting higher Basidiomycetes mushrooms isolated from Eucalyptus plantations in Uruguay was studied using bacterial and fungal phytopathogens as targets. Fifty-one extracts from mycelia and growth broth were prepared from higher Basidiomycetes mushrooms, from which eight extracts (from Ganoderma resinaceum, Laetiporus sulphureus, Dictyopanus pusillus, and Bjerkandera adusta) showed antimicrobial activity against Xanthomonas vesicatoria, Aspergillus oryzae, Penicillium expansum, Botrytis cinerea, and Rhizopus stolonifer as assayed in the qualitative test. The minimum inhibitory concentration (MIC) for those fungal extracts was determined and the results showed that L. sulphureus deserved further study, with low MIC values against X. vesicatoria. The antimicrobial activity of L. sulphureus culture broth extracts grown under different culture conditions was evaluated against X. vesicatoria. From the results of these assays, larger-scale cultures for the production of the compound(s) with antimicrobial activity should be performed using malt extract broth, at pH 5, at 20°C and static culture conditions.
authors.affiliation: Laboratorio de Farmacognosia, Departamento de Quimica Organica, Facultad de Quimica, Universidad de la Republica, Montevideo, Uruguay.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Barneche
authors.affiliation: Laboratorio de Microbiologia, Departamento de Biociencias, Facultad de Quimica, Universidad de la Republica, Montevideo, Uruguay.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Jorcin
authors.affiliation: Laboratorio de Microbiologia, Instituto de Quimica Biologica, Facultad de Ciencias, Facultad de Quimica, Universidad de la Republica, Montevideo, Uruguay.
authors.forename: Gianna
authors.initials: G
authors.lastname: Cecchetto
authors.affiliation: Laboratorio de Microbiologia, Departamento de Biociencias, Facultad de Quimica, Universidad de la Republica, Montevideo, Uruguay.
authors.forename: María Pía
authors.initials: MP
authors.lastname: Cerdeiras
authors.affiliation: Laboratorio de Farmacognosia, Departamento de Quimica Organica, Facultad de Quimica, Universidad de la Republica, Montevideo, Uruguay.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Vázquez
authors.affiliation: Laboratorio de Microbiologia, Departamento de Biociencias, Facultad de Quimica, Universidad de la Republica, Montevideo, Uruguay.
authors.forename: Silvana
authors.initials: S
authors.lastname: Alborés
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001236:Aspergillus oryzae / Q000187:drug effects; D001487:Basidiomycota / Q000737:chemistry*; D020171:Botrytis / Q000187:drug effects; D045424:Complex Mixtures / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D025282:Mycelium / Q000737:chemistry; D010407:Penicillium / Q000187:drug effects; D012233:Rhizopus / Q000187:drug effects; D014583:Uruguay; D014934:Wood / Q000382:microbiology; D014974:Xanthomonas / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D045424:Complex Mixtures