title: Chemical Screening in Zebrafish.
abstract: Phenotypic small molecule screens in zebrafish have gained popularity as an unbiased approach to probe biological processes. In this chapter we outline basic methods for performing chemical screens with larval zebrafish including breeding large numbers of embryos, plating larval fish into multi-well dishes, and adding small molecules to these wells. We also highlight important considerations when designing and interpreting the results of a phenotypic screen and possible follow-up approaches, including popular methods used to identify the mechanism of action of a chemical compound.
authors.affiliation: Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, MA, 02129, USA.|Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA, 02115, USA.|Broad Institute, 7 Cambridge Center, Cambridge, MA, 02142, USA.
authors.forename: Colleen A
authors.initials: CA
authors.lastname: Brady
authors.affiliation: Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, MA, 02129, USA.|Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA, 02115, USA.|Broad Institute, 7 Cambridge Center, Cambridge, MA, 02142, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Rennekamp
authors.affiliation: Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, MA, 02129, USA. PETERSON@CVRC.MGH.HARVARD.EDU.|Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA, 02115, USA. PETERSON@CVRC.MGH.HARVARD.EDU.|Broad Institute, 7 Cambridge Center, Cambridge, MA, 02142, USA. PETERSON@CVRC.MGH.HARVARD.EDU.
authors.forename: Randall T
authors.initials: RT
authors.lastname: Peterson
mesh_terms: D000818:Animals; D055808:Drug Discovery / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000502:physiology; D007814:Larva / Q000187:drug effects / Q000502:physiology; D010641:Phenotype; D054852:Small Molecule Libraries / Q000494:pharmacology; D015027:Zebrafish / Q000196:embryology / Q000502:physiology
chemical_list: D054852:Small Molecule Libraries
keywords: Multi-well plates; Phenotypic screening; Small molecules