title: Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy.
abstract: Heterogeneous and unpredictable environmental insult, disease, or trauma can affect the integrity and function of neuronal circuits, leading to irreversible neural dysfunction. The peripheral nervous system can robustly regenerate axons after damage to recover the capacity to transmit sensory information to the brain. The mechanisms that allow axonal repair remain incompletely understood. Here we present a preparation in zebrafish that combines laser microsurgery of sensory axons and videomicroscopy of neurons in multicolor transgenic specimens. This simple protocol allows controlled damage of axons and dynamic high-resolution visualization and quantification of repair.
authors.affiliation: Sensory Biology and Organogenesis, Helmholtz Zentrum München, Ingolstädter Landstr. 1, Munich, D-85764, Germany.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Sensory Biology and Organogenesis, Helmholtz Zentrum München, Ingolstädter Landstr. 1, Munich, D-85764, Germany. hernan.lopez-schier@helmholtz-muenchen.de.
authors.forename: Hernán
authors.initials: H
authors.lastname: López-Schier
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D001921:Brain / Q000166:cytology; D018715:Microscopy, Video / Q000379:methods*; D008866:Microsurgery / Q000379:methods*; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000166:cytology; D017933:Peripheral Nervous System / Q000166:cytology; D015027:Zebrafish
keywords: Fluorescent protein; Live imaging; Microscopy; Zebrafish