title: Advances in high-throughput and high-efficiency chiral liquid chromatographic separations.
abstract: The need for improved liquid chromatographic chiral separations has led to the advancement of chiral screening techniques as well as the development of new, high efficiency chiral separation methods and stationary phases. This review covers these advancements, which primarily occurred over the last 15 years. High throughput techniques include multi-column screening units, multiple injection sequences, and fast gradient SFC screening. New separation methods and column technologies that aim at high efficiency chiral separations include the use of achiral UHPLC (i.e. sub-2μm) columns for separating derivatized chiral analytes or using chiral additives in the run buffer, UHPLC chiral stationary phases, and superficially porous particle based chiral stationary phases. Finally, the enhancement of chiral separations through these new technologies requires that certain instrumental considerations be made. Future directions in continuing to improve chiral separations are also discussed.
authors.affiliation: The University of Texas at Arlington, 700 Planetarium Place, Arlington, TX 76019, USA.
authors.forename: Darshan C
authors.initials: DC
authors.lastname: Patel
authors.affiliation: The University of Texas at Arlington, 700 Planetarium Place, Arlington, TX 76019, USA.
authors.forename: M Farooq
authors.initials: MF
authors.lastname: Wahab
authors.affiliation: The University of Texas at Arlington, 700 Planetarium Place, Arlington, TX 76019, USA.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Armstrong
authors.affiliation: The University of Texas at Arlington, 700 Planetarium Place, Arlington, TX 76019, USA. Electronic address: zachary.breitbach@mavs.uta.edu.
authors.forename: Zachary S
authors.initials: ZS
authors.lastname: Breitbach
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000639:trends; D002853:Chromatography, Liquid / Q000639:trends*; D006801:Humans; D016062:Porosity; D013237:Stereoisomerism
keywords: HPLC and SFC enantiomeric separations; High-efficiency chiral separations; High-throughput chromatography; Instrument optimizations; Superficially porous particles; Ultrafast separations