title: High throughput screening of starch structures using carbohydrate microarrays.
abstract: In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches.
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.
authors.forename: Vanja
authors.initials: V
authors.lastname: Tanackovic
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.
authors.forename: Maja Gro
authors.initials: MG
authors.lastname: Rydahl
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.
authors.forename: Henriette Lodberg
authors.initials: HL
authors.lastname: Pedersen
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.
authors.forename: Mohammed Saddik
authors.initials: MS
authors.lastname: Motawia
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.
authors.forename: Shahnoor Sultana
authors.initials: SS
authors.lastname: Shaik
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.
authors.forename: Maria Dalgaard
authors.initials: MD
authors.lastname: Mikkelsen
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.
authors.forename: Susanne Langgaard
authors.initials: SL
authors.lastname: Krunic
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.
authors.forename: Jonatan Ulrik
authors.initials: JU
authors.lastname: Fangel
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.
authors.forename: William George Tycho
authors.initials: WG
authors.lastname: Willats
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.
authors.forename: Andreas
authors.initials: A
authors.lastname: Blennow
mesh_terms: D000688:Amylose / Q000032:analysis*; D001234:Aspergillus niger / Q000737:chemistry*; D002236:Carbohydrate Conformation; D005656:Fungal Proteins / Q000737:chemistry*; D001467:Hordeum / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods*
chemical_list: D005656:Fungal Proteins; D037102:Lectins; D000688:Amylose