title: Quantitative Functional Morphology by Imaging Flow Cytometry.
abstract: This chapter describes advantages and limitations of imaging flow cytometry (IFC) based on Imagestream instrumentation using a hybrid approach of morphometric measurement and quantitation of multiparametric fluorescent intensities' distribution in cells and particles. Brief comparison is given of IFC with conventional flow cytometry and fluorescent microscopy. Some future directions of the IFC technology are described and discussed.
authors.affiliation: Department of Cell Biology and Histology, M.V. Lomonosov Moscow State University, Moscow, 119992, Russia. vorob@libro.genebee.msu.ru.|A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Moscow, 119992, Russia. vorob@libro.genebee.msu.ru.
authors.forename: Ivan A
authors.initials: IA
authors.lastname: Vorobjev
authors.affiliation: Cellular and Molecular Medicine Program, Boston Childrens Hospital, Boston, MA, 02115, USA. natasha.barteneva@childrens.harvard.edu.|Department of Pediatrics, Harvard Medical School, Boston, MA, 02115, USA. natasha.barteneva@childrens.harvard.edu.
authors.forename: Natasha S
authors.initials: NS
authors.lastname: Barteneva
mesh_terms: D005434:Flow Cytometry / Q000266:history / Q000295:instrumentation / Q000379:methods*; D049673:History, 20th Century; D049674:History, 21st Century; D019044:Image Cytometry / Q000266:history / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence; D012984:Software
keywords: Autofluorescence; Automated image analysis; Flow cytometry; Imaging flow cytometry; Quantitative morphology; Spectral compensation