title: Bioremediation of domestic and industrial wastewaters integrated with enhanced biodiesel production using novel oleaginous microalgae.
abstract: The study illustrates the synergistic potential of novel microalgal, Chlamydomonas debaryana IITRIND3, for phycoremediation of domestic, sewage, paper mill and dairy wastewaters and then subsequent utilisation of its biomass for biodiesel production. Among these wastewaters, maximum lipid productivity (87.5 ± 2.3 mg L-1day-1) was obtained in dairy wastewater with removal efficiency of total nitrogen, total phosphorous, chemical oxygen demand and total organic carbon to be 87.56, 82.17, 78.57 and 85.97 %, respectively. Metal ions such as sodium, calcium, potassium and magnesium were also removed efficiently from the wastewaters tested. Pigment analysis revealed loss of chlorophyll a while increase in carotenoid content in algal cells cultivated in different wastewaters. Biochemical data of microalgae grown in different wastewaters showed reduction in protein content with an increase in carbohydrate and lipid contents. The major fatty acids in algal cells grown in dairy wastewater were C14:0, C16:0, C16:1, C18:0, C18:2 and C18:3. The physical properties of biodiesel derived from microalgae grown in dairy wastewater were in compliance with the ASTM D6751 and EN 14214 fuel standards and were comparable to plant oil methyl esters.
authors.affiliation: Molecular Microbiology Laboratory, Biotechnology Department, IITR, Roorkee, Uttarakhand, 247667, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Arora
authors.affiliation: Molecular Microbiology Laboratory, Biotechnology Department, IITR, Roorkee, Uttarakhand, 247667, India.
authors.forename: Alok
authors.initials: A
authors.lastname: Patel
authors.affiliation: Molecular Microbiology Laboratory, Biotechnology Department, IITR, Roorkee, Uttarakhand, 247667, India.
authors.forename: Km
authors.initials: K
authors.lastname: Sartaj
authors.affiliation: Molecular Microbiology Laboratory, Biotechnology Department, IITR, Roorkee, Uttarakhand, 247667, India.
authors.forename: Parul A
authors.initials: PA
authors.lastname: Pruthi
authors.affiliation: Molecular Microbiology Laboratory, Biotechnology Department, IITR, Roorkee, Uttarakhand, 247667, India. vikasfbs@gmail.com.
authors.forename: Vikas
authors.initials: V
authors.lastname: Pruthi
mesh_terms: D001673:Biodegradation, Environmental; D056804:Biofuels; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D002696:Chlamydomonas / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D003612:Dairying; D007220:Industrial Waste; D050356:Lipid Metabolism; D008670:Metals / Q000378:metabolism; D058086:Microalgae / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D056804:Biofuels; D002734:Chlorophyll; D000077194:Chlorophyll A; D007220:Industrial Waste; D008670:Metals; D009584:Nitrogen; D010758:Phosphorus; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Biodiesel; Bioremediation; Lipid productivity; Microalgae; Wastewater