title: Quantification of Total and 2-LTR (Long terminal repeat) HIV DNA, HIV RNA and Herpesvirus DNA in PBMCs.
abstract: Almost all individuals infected with human immunodeficiency virus (HIV) are also infected with cytomegalovirus (CMV) and Epstein Barr virus (EBV). The aims of our studies have included characterizing and measuring the latent HIV reservoir and understanding the association between asymptomatic replication of CMV (and other herpesvirus, including EBV) and this HIV reservoir (Gianellaet al., 2014). This protocol was designed to simultaneously co-extract DNA and RNA from the same peripheral blood mononuclear cell (PBMC) aliquot and quantify HIV, CMV and EBV DNA, as well as HIV RNA using droplet digital PCR (ddPCR). For collection and processing of male genital secretions and quantification of HIV RNA and DNA from seven human herpesviruses from seminal plasma, refer to protocol "Quantification of HIV RNA and Human Herpesvirus DNA in Seminal Plasma" (Vargas-Meneseset al., 2015).
authors.affiliation: University of California San Diego, La Jolla, USA.
authors.forename: Marta
authors.initials: M
authors.lastname: Massanella
authors.affiliation: University of California San Diego, La Jolla, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Gianella
authors.affiliation: University of California San Diego, La Jolla, USA.
authors.forename: Steven M
authors.initials: SM
authors.lastname: Lada
authors.affiliation: University of California San Diego, La Jolla, USA; Veterans Affairs San Diego Healthcare System, La Jolla, USA.
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Richman
authors.affiliation: University of California San Diego, La Jolla, USA.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Strain