title: Genome-wide transcriptomic analysis uncovers the molecular basis underlying early flowering and apetalous characteristic in Brassica napus L.
abstract: Floral transition and petal onset, as two main aspects of flower development, are crucial to rapeseed evolutionary success and yield formation. Currently, very little is known regarding the genetic architecture that regulates flowering time and petal morphogenesis in Brassica napus. In the present study, a genome-wide transcriptomic analysis was performed with an absolutely apetalous and early flowering line, APL01, and a normally petalled line, PL01, using high-throughput RNA sequencing. In total, 13,205 differential expressed genes were detected, of which 6111 genes were significantly down-regulated, while 7094 genes were significantly up-regulated in the young inflorescences of APL01 compared with PL01. The expression levels of a vast number of genes involved in protein biosynthesis were altered in response to the early flowering and apetalous character. Based on the putative rapeseed flowering genes, an early flowering network, mainly comprised of vernalization and photoperiod pathways, was built. Additionally, 36 putative upstream genes possibly governing the apetalous character of line APL01 were identified, and six genes potentially regulating petal origination were obtained by combining with three petal-related quantitative trait loci. These findings will facilitate understanding of the molecular mechanisms underlying floral transition and petal initiation in B. napus.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.|Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture/Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors.forename: Kunjiang
authors.initials: K
authors.lastname: Yu
authors.affiliation: Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture/Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture/Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture/Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors.forename: Song
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture/Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Peng
authors.affiliation: Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture/Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors.forename: Hongge
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture/Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture/Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors.forename: Maolong
authors.initials: M
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Chu
authors.affiliation: Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture/Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors.forename: Jiefu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Rongzhan
authors.initials: R
authors.lastname: Guan
mesh_terms: D029688:Brassica napus / Q000235:genetics*; D035264:Flowers / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D055106:Genome-Wide Association Study; D059014:High-Throughput Nucleotide Sequencing; D010641:Phenotype; D017440:Photoperiod; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D017423:Sequence Analysis, RNA
chemical_list: D010940:Plant Proteins