title: Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems.
abstract: With the development of high-throughput sequencing technology in the post-genomic era, researchers have concentrated their efforts on elucidating the relationships between genes and their corresponding functions. Recently, important progress has been achieved in the generation of genetically modified mice based on CRISPR/Cas9 and haploid embryonic stem cell (haESC) approaches, which provide new platforms for gene function analysis, human disease modeling, and gene therapy. Here, we review the CRISPR/Cas9 and haESC technology for the generation of genetically modified mice and discuss the key challenges in the application of these approaches.
authors.affiliation: College of Life Science of Shaoxing University, Shaoxing Zhejiang 312000, China;State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China;Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China. Lifangj@sohu.com.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China;Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D064113:CRISPR-Cas Systems / Q000235:genetics*; D053595:Embryonic Stem Cells / Q000378:metabolism*; D000072669:Gene Editing / Q000379:methods*; D006238:Haploidy; D006801:Humans; D051379:Mice
keywords: CRISPR/Cas9; Genetic modification; Haploid embryonic stem cells; Mouse