title: Advances and perspectives in the application of CRISPR/Cas9 in insects.
abstract: Insects compose more than half of all living organisms on earth, playing essential roles in global ecosystems and forming complex relationships with humans. Insect research has significant biological and practical importance. However, the application of genetic manipulation technology has long been restricted to several model insects only, such as gene knockout in Drosophila, which has severely restrained the development of insect biology research. Recently, with the increase in the release of insect genome data and the introduction of the CRISPR/Cas9 system for efficient genetic modification, it has been possible to conduct meaningful functional studies in a broad array of insect species. Here, we summarize the advances in CRISPR/Cas9 in different insect species, discuss methods for its promotion, and consider its application in future insect studies. This review provides detailed information about the application of the CRISPR/Cas9 system in insect research and presents possible ways to improve its use in functional studies and insect pest control.
authors.affiliation: State Key Laboratory of Genetic Resources & Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China;University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Hetao, Bayannaoer Inner Mongolia 015000, China.
authors.forename: Gui
authors.initials: G
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Genetic Resources & Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China;Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650223, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Genetic Resources & Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiang
authors.affiliation: State Key Laboratory of Genetic Resources & Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China. wwang@mail.kiz.ac.cn.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems / Q000235:genetics*; D004247:DNA / Q000235:genetics; D000072669:Gene Editing / Q000379:methods*; D007313:Insecta / Q000235:genetics*; D012313:RNA / Q000235:genetics
chemical_list: D012313:RNA; D004247:DNA
keywords: CRISPR/Cas9; Gene editing; Insect transgenesis