title: Desulfotomaculum aquiferis sp. nov. and Desulfotomaculum profundi sp. nov., isolated from a deep natural gas storage aquifer.
abstract: Two novel strictly anaerobic bacteria, strains Bs105T and Bs107T, were isolated from a deep aquifer-derived hydrocarbonoclastic community. The cells were rod-shaped, not motile and had terminal spores. Phylogenetic affiliation and physiological properties revealed that these isolates belong to two novel species of the genus Desulfotomaculum. Optimal growth temperatures for strains Bs105T and Bs107T were 42 and 45 °C, respectively. The estimated G+C content of the genomic DNA was 42.9 and 48.7 mol%. For both strains, the major cellular fatty acid was palmitate (C16 : 0). Specific carbon fatty acid signatures of Gram-positive bacteria (iso-C17 : 0) and sulfate-reducing bacteria (C17 : 0cyc) were also detected. An insertion was revealed in one of the two 16S rRNA gene copies harboured by strain Bs107T. Similar insertions have previously been highlighted among moderately thermophilic species of the genus Desulfotomaculum. Both strains shared the ability to oxidize aromatic acids (Bs105T: hydroquinone, acetophenone, para-toluic acid, 2-phenylethanol, trans-cinnamic acid, 4-hydroxybenzaldehyde, benzyl alcohol, benzoic acid 4-hydroxybutyl ester; Bs107T: ortho-toluic acid, benzoic acid 4-hydroxybutyl ester). The names Desulfotomaculum aquiferis sp. nov. and Desulfotomaculum profundi sp. nov. are proposed for the type strains Bs105T (=DSM 24088T=JCM 31386T) and Bs107T (=DSM 24093T=JCM 31387T).
authors.affiliation: Université de Pau et des Pays de l'Adour, CNRS, IPREM UMR 5254, Equipe Environnement et Microbiologie, Pau 64013, France.|School of Earth and Ocean Sciences, Main building, Park Place, Cardiff University, Cardiff CF10 3AT, UK.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Berlendis
authors.affiliation: Université de Pau et des Pays de l'Adour, CNRS, IPREM UMR 5254, Equipe Environnement et Microbiologie, Pau 64013, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Ranchou-Peyruse
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288 Marseille Cedex 09, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
authors.affiliation: APESA, Hélioparc, 2 avenue P. Angot, F-64053 PAU Cedex 09, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Lascourrèges
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288 Marseille Cedex 09, France.
authors.forename: Manon
authors.initials: M
authors.lastname: Joseph
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288 Marseille Cedex 09, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
authors.affiliation: TIGF - Transport et Infrastructures Gaz France, 40 Avenue de l'Europe, CS20522, Pau 64000, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Aüllo
authors.affiliation: Storengy - Geosciences Department, Bois-Colombes, France.
authors.forename: David
authors.initials: D
authors.lastname: Dequidt
authors.affiliation: Université de Pau et des Pays de l'Adour, CNRS, IPREM UMR 5254, Equipe Environnement et Microbiologie, Pau 64013, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Magot
authors.affiliation: Université de Pau et des Pays de l'Adour, CNRS, IPREM UMR 5254, Equipe Environnement et Microbiologie, Pau 64013, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Ranchou-Peyruse
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D045859:Desulfotomaculum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D005602:France; D060587:Groundwater / Q000382:microbiology*; D059206:Natural Gas; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D059206:Natural Gas; D012336:RNA, Ribosomal, 16S