title: 3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
abstract: Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein-ligand complexes.
authors.affiliation: State Key Laboratory and Research Institute of Elemento-Organic Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China. xunchegnsu@nankai.edu.cn.
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Pan
authors.affiliation: State Key Laboratory and Research Institute of Elemento-Organic Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China. xunchegnsu@nankai.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China. conggangli@wipm.ac.cn.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China. conggangli@wipm.ac.cn.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China. conggangli@wipm.ac.cn.
authors.forename: Conggang
authors.initials: C
authors.lastname: Li
authors.affiliation: Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia. t.huber@anu.edu.au.
authors.forename: Thomas
authors.initials: T
authors.lastname: Huber
authors.affiliation: State Key Laboratory and Research Institute of Elemento-Organic Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China. xunchegnsu@nankai.edu.cn.
authors.forename: Xun-Cheng
authors.initials: XC
authors.lastname: Su