title: Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae.
abstract: Zebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish allow for multiple subject, multidimensional, and longitudinal imaging experiments. This chapter describes protocols for infecting zebrafish larvae with their natural pathogen Mycobacterium marinum, rapid short-term imaging, long-term extended imaging, and drug treatment assays. These protocols can be easily adapted to image and manipulate host interactions with other pathogens.
authors.affiliation: Department of Molecular Genetics and Microbiology, Center for Host-Microbial Interactions, Duke University Medical Center, 219 Jones Building, Box 3020 DUMC, Durham, NC, 27710, USA.|University Program in Genetics and Genomics, Duke University, Durham, NC, 27710, USA.
authors.forename: Molly A
authors.initials: MA
authors.lastname: Matty
authors.affiliation: Department of Molecular Genetics and Microbiology, Center for Host-Microbial Interactions, Duke University Medical Center, 219 Jones Building, Box 3020 DUMC, Durham, NC, 27710, USA.
authors.forename: Stefan H
authors.initials: SH
authors.lastname: Oehlers
authors.affiliation: Department of Molecular Genetics and Microbiology, Center for Host-Microbial Interactions, Duke University Medical Center, 219 Jones Building, Box 3020 DUMC, Durham, NC, 27710, USA. david.tobin@duke.edu.|Department of Immunology, Duke University Medical Center, Durham, NC, 27710, USA. david.tobin@duke.edu.
authors.forename: David M
authors.initials: DM
authors.lastname: Tobin
mesh_terms: D000818:Animals; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D007113:Immunity, Innate / Q000235:genetics / Q000502:physiology; D007814:Larva / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D019910:Mycobacterium marinum / Q000276:immunology / Q000472:pathogenicity; D015027:Zebrafish / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology
keywords: Drug treatment; Infection; Innate immunity; Mycobacteria; Time-lapse imaging; Zebrafish