title: Effect of physicochemical properties of a cement based on silicocarnotite/calcium silicate on in vitro cell adhesion and in vivo cement degradation.
abstract: A silicon calcium phosphate cement (Si-CPC) was developed to produce a composite of calcium phosphate and calcium silicate. The silicon cements prepared with low silicon (Si) content were composed of crystalline phases of brushite and silicocarnotite. However, the cements prepared with high Si content were mainly composed of amorphous phases of silicocarnotite, hydroxyapatite and calcium silicate. The cement porosity was about 40% with a shift of the average pore diameter to the nanometric range with increasing Si content. Interestingly, this new cement system provides a matrix with a high specific surface area of up to 29 m(2) g(-1). The cytocompatibility of the new Si-doped cements was tested with a human osteoblast-like cell line (MG-63) showing an enhancement of cell proliferation (up to threefold) when compared with unsubstituted material. Cements with a high silica content also improved the cell attachment. The in vivo results indicated that Si-CPCs induce the formation of new bone tissue, and modify cement resorption. We conclude that this cement provides an optimal environment to enhance osteoblast growth and proliferation that could be of interest in bone engineering.
authors.affiliation: Faculty of Pharmacy, Department of Physical-Chemistry II, Complutense University of Madrid, Madrid 28040, Spain. Faculty of Odontology, Department of Stomatology III, UCM, Madrid 28040, Spain.
authors.forename: Julia Lucas
authors.initials: JL
authors.lastname: Aparicio
authors.forename: Carmen
authors.initials: C
authors.lastname: Rueda
authors.forename: Ángel
authors.initials: Á
authors.lastname: Manchón
authors.forename: Andrea
authors.initials: A
authors.lastname: Ewald
authors.forename: Uwe
authors.initials: U
authors.lastname: Gbureck
authors.forename: Mohammad Hamdan
authors.initials: MH
authors.lastname: Alkhraisat
authors.forename: Luis Blanco
authors.initials: LB
authors.lastname: Jerez
authors.forename: Enrique López
authors.initials: EL
authors.lastname: Cabarcos
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D011817:Rabbits; D012823:Silicate Cement / Q000737:chemistry / Q000494:pharmacology*; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology*; D023822:Tissue Engineering
chemical_list: D017610:Calcium Compounds; D002130:Calcium Phosphates; D017640:Silicates; C584000:silicocarnotite; D012823:Silicate Cement; C031293:calcium silicate