title: Sex-Dependent Effects of Dietary Genistein on Echocardiographic Profile and Cardiac GLUT4 Signaling in Mice.
abstract: This study aimed to determine whether genistein diet resulted in changes in cardiac function, using echocardiography, and expression of key proteins involved in glucose uptake by the myocardium. Intact male and female C57BL/6J mice (aged 4-6 weeks) were fed either 600 mg genistein/kg diet (600 G) or 0 mg genistein/kg diet (0 G) for 4 weeks. Echocardiography data revealed sex-dependent differences in the absence of genistein: compared to females, hearts from males exhibited increased systolic left ventricle internal dimension (LVIDs), producing a decrease in function, expressed as fractional shortening (FS). Genistein diet also induced echocardiographic changes in function: in female hearts, 600G induced a 1.5-fold (P < 0.05) increase in LVIDs, resulting in a significant decrease in FS and whole heart surface area when compared to controls (fed 0 G). Genistein diet increased cardiac GLUT4 protein expression in both males (1.51-fold, P < 0.05) and females (1.76-fold, P < 0.05). However, no effects on the expression of notable intracellular signaling glucose uptake-regulated proteins were observed. Our data indicate that consumption of genistein diet for 4 weeks induces echocardiographic changes in indices of systolic function in females and has beneficial effects on cardiac GLUT4 protein expression in both males and females.
authors.affiliation: Department of Physiology, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USA.
authors.forename: Lana
authors.initials: L
authors.lastname: Leung
authors.affiliation: Department of Physiology, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USA.
authors.forename: Joshua B
authors.initials: JB
authors.lastname: Martin
authors.affiliation: Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, 350 West Thomas Road, Phoenix, AZ 85013, USA.
authors.forename: Todd
authors.initials: T
authors.lastname: Lawmaster
authors.affiliation: Department of Physiology, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USA.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Arthur
authors.affiliation: Department of Physiology, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USA.
authors.forename: Tom L
authors.initials: TL
authors.lastname: Broderick
authors.affiliation: Department of Physiology, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USA.
authors.forename: Layla
authors.identifier: 0000-0001-9309-6213
authors.initials: L
authors.lastname: Al-Nakkash