title: Proteomic Profiling and the Predicted Interactome of Host Proteins in Compatible and Incompatible Interactions Between Soybean and Fusarium virguliforme.
abstract: Sudden death syndrome (SDS) is a complex of two diseases of soybean (Glycine max), caused by the soil borne pathogenic fungus Fusarium virguliforme. The root rot and leaf scorch diseases both result in significant yield losses worldwide. Partial SDS resistance has been demonstrated in multiple soybean cultivars. This study aimed to highlight proteomic changes in soybean roots by identifying proteins which are differentially expressed in near isogenic lines (NILs) contrasting at the Rhg1/Rfs2 locus for partial resistance or susceptibility to SDS. Two-dimensional gel electrophoresis resolved approximately 1000 spots on each gel; 12 spots with a significant (P < 0.05) difference in abundance of 1.5-fold or more were picked, trypsin-digested, and analyzed using quadruple time-of-flight tandem mass spectrometry. Several spots contained more than one protein, so that 18 distinct proteins were identified overall. A functional analysis performed to categorize the proteins depicted that the major pathways altered by fungal infection include disease resistance, stress tolerance, and metabolism. This is the first report which identifies proteins whose abundances are altered in response to fungal infection leading to SDS. The results provide valuable information about SDS resistance in soybean plants, and plant partial resistance responses in general. More importantly, several of the identified proteins could be good candidates for the development of SDS-resistant soybean plants.
authors.affiliation: Department of Plant Sciences, University of California, Davis, California, 95616, USA.
authors.forename: M Javed
authors.initials: MJ
authors.lastname: Iqbal
authors.affiliation: Department of Biology, SBA School of Science and Engineering, Lahore University of Management Sciences, Lahore, 54792, Pakistan.|Department of Biological Sciences, Columbia University, New York, NY, 10027, USA.
authors.forename: Maryam
authors.initials: M
authors.lastname: Majeed
authors.affiliation: Department of Biology, SBA School of Science and Engineering, Lahore University of Management Sciences, Lahore, 54792, Pakistan.
authors.forename: Maheen
authors.initials: M
authors.lastname: Humayun
authors.affiliation: Department of Molecular Biology, Microbiology, and Biochemistry, Genomics Core Facility and Center for Excellence in Soybean Research, Teaching, and Outreach, and Department of Plant Biology, Southern Illinois University, Carbondale, Illinois, 62901, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Lightfoot
authors.affiliation: Department of Biology, SBA School of Science and Engineering, Lahore University of Management Sciences, Lahore, 54792, Pakistan. ahmed.afzal@lums.edu.pk.|Department of Molecular Biology, Microbiology, and Biochemistry, Genomics Core Facility and Center for Excellence in Soybean Research, Teaching, and Outreach, and Department of Plant Biology, Southern Illinois University, Carbondale, Illinois, 62901, USA. ahmed.afzal@lums.edu.pk.
authors.forename: Ahmed J
authors.initials: AJ
authors.lastname: Afzal
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005670:Fusarium / Q000502:physiology*; D054884:Host-Pathogen Interactions; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000382:microbiology; D011485:Protein Binding; D025941:Protein Interaction Mapping; D040901:Proteomics / Q000379:methods*; D013025:Glycine max / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D010940:Plant Proteins
keywords: 2D gel electrophoresis; Proteomics; Rhg1/Rfs2 locus; Soybean; Sudden death syndrome