title: Column chromatography as a useful step in purification of diatom pigments.
abstract: Fucoxanthin, diadinoxanthin and diatoxanthin are carotenoids found in brown algae and most other heterokonts. These pigments are involved in photosynthetic and photoprotective reactions, and they have many potential health benefits. They can be extracted from diatom Phaeodactylum tricornutum by sonication, extraction with chloroform : methanol and preparative thin layer chromatography. We assessed the utility of an additional column chromatography step in purification of these pigments. This novel addition to the isolation protocol increased the purity of fucoxanthin and allowed for concentration of diadinoxanthin and diatoxanthin before HPLC separation. The enhanced protocol is useful for obtaining high purity pigments for biochemical studies.
authors.affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
authors.forename: Wiktor
authors.initials: W
authors.lastname: Tokarek
authors.affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Listwan
authors.affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Pagacz
authors.affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Leśniak
authors.affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Latowski
mesh_terms: D002725:Chloroform / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D017377:Diatoms / Q000737:chemistry*; D059625:Liquid-Liquid Extraction; D000432:Methanol / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013010:Sonication; D024341:Xanthophylls / Q000302:isolation & purification*
chemical_list: D012997:Solvents; D024341:Xanthophylls; C025164:fucoxanthin; C033808:diadinoxanthin; C103105:diatoxanthin; D002725:Chloroform; D000432:Methanol