title: Genomic Amplifications Cause False Positives in CRISPR Screens.
abstract: In CRISPR-based screens for essential genes, Munoz and colleagues and Aguirre and colleagues show that gene-independent targeting of genomic amplifications in human cancer cell lines reduces proliferation or survival. The correlation between CRISPR target site copy number and lethality demonstrates the need for scrutiny and complementary approaches to rule out off-target effects and false positives in CRISPR screens. Cancer Discov; 6(8); 824-6. ©2016 AACR.See related article by Munoz et al., p. 900See related article by Aguirre et al., p. 914.
authors.affiliation: RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, Massachusetts.
authors.forename: Ankur
authors.initials: A
authors.lastname: Sheel
authors.affiliation: RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, Massachusetts. Program in Molecular Medicine and Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts. Wen.Xue@umassmed.edu.
authors.forename: Wen
authors.initials: W
authors.lastname: Xue
mesh_terms: D064113:CRISPR-Cas Systems; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D005784:Gene Amplification; D056726:Genetic Association Studies / Q000379:methods / Q000592:standards; D015894:Genome, Human; D023281:Genomics / Q000379:methods / Q000592:standards; D006801:Humans; D009369:Neoplasms / Q000235:genetics; D015203:Reproducibility of Results