title: CaMKII-mediated displacement of AIDA-1 out of the postsynaptic density core.
abstract: Ankyrin repeat and sterile alpha motif domain-containing protein 1B (ANKS1B, also known as AIDA-1) is a major component of the postsynaptic density (PSD) in excitatory neurons where it concentrates at the electron-dense core under basal conditions and moves out during activity. This study investigates the molecular mechanism underlying activity-induced displacement of AIDA-1. Experiments with PSD fractions from brain indicate phosphorylation of AIDA-1 upon activation of endogenous CaMKII. Immuno-electron microscopy studies show that treatment of hippocampal neurons with NMDA results in an ~ 30 nm shift in the median distance of the AIDA-1 label from the postsynaptic membrane, an effect that is blocked by the CaMKII inhibitor tatCN21. CaMKII-mediated redistribution of AIDA-1 is similar to that observed for SynGAP. CaMKII-mediated removal of two abundant PSD-95-binding proteins from the PSD core during activity is expected to initiate a molecular reorganization at the PSD.
authors.affiliation: Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Ayse
authors.initials: A
authors.lastname: Dosemeci
authors.affiliation: Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Dana
authors.initials: D
authors.lastname: Toy
authors.affiliation: Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Amelia
authors.initials: A
authors.lastname: Burch
authors.affiliation: Department of Pharmacology, School of Medicine, University of Colorado Denver, Aurora, CO, USA.
authors.forename: K Ulrich
authors.initials: KU
authors.lastname: Bayer
authors.affiliation: EM Facility, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Jung-Hwa
authors.initials: JH
authors.lastname: Tao-Cheng
mesh_terms: D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D016253:Microscopy, Immunoelectron; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D010455:Peptides / Q000494:pharmacology; D010766:Phosphorylation; D057907:Post-Synaptic Density / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats
chemical_list: C000607858:Anks1b protein, rat; D002352:Carrier Proteins; D010455:Peptides; C000616211:tatCN21 peptide; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2
keywords: AIDA-1; CaMKII; postsynaptic density