title: Miconia sp. Increases mRNA Levels of PPAR Gamma and Inhibits Alpha Amylase and Alpha Glucosidase.
abstract: Diabetes mellitus is a public health problem worldwide. For this reason, ethanolic extract of Miconia sp. from Oaxaca, Mexico, was selected in search of an alternative against this disease. The effect of Miconia sp. on mRNA expression of PPARγ on cell line 3T3-L1, its effect on alpha amylase and alpha glucosidase, lipid accumulation during adipogenesis, and cell viability on VERO cells were evaluated. The mRNA levels of PPARγ increased on 1.393 ± 0.008 folds, lipid accumulation was increased by 29.55% with Miconia sp. extract and 34.57% with rosiglitazone, and α-amylase and α-glycosidase were inhibited with IC50 values from 28.23 ± 2.15 μg/mL and 1.95 ± 0.15 μg/mL, respectively; the IC50 on antiproliferative activity on VERO cells was 314.54 ± 45.40 μg/mL. In case of α-amylase and α-glycosidase assays, IC50 (inhibitory concentration 50) refers to necessary extract amounts to inhibit 50% of enzymatic activity. On the other hand, on antiproliferative activity, IC50 (inhibitory concentration 50) refers to necessary extract amounts to inhibit 50% of cell proliferation. It was concluded that the compounds present in Miconia sp. ethanolic extract increase mRNA expression of PPARγ, inhibit α-amylase and α-glucosidase, and increase lipid accumulation. It constitutes an alternative as adjuvant in diabetes mellitus treatment; therefore, we recommend continuing identifying the compounds responsible for its promising in vivo antidiabetic activity.
authors.affiliation: Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Pedro de Alba, s/n, Ciudad Universitaria, 66455 San Nicolas de los Garza, NL, Mexico.
authors.forename: David Mizael
authors.initials: DM
authors.lastname: Ortíz-Martinez
authors.affiliation: Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Pedro de Alba, s/n, Ciudad Universitaria, 66455 San Nicolas de los Garza, NL, Mexico.
authors.forename: Catalina
authors.initials: C
authors.lastname: Rivas-Morales
authors.affiliation: Facultad de Odontologia, Universidad Autonoma de Nuevo Leon, Eduardo Aguirre Pequeño y Silao, s/n, Mitras Centro, 64460 Monterrey, NL, Mexico.
authors.forename: Myriam Angelica
authors.initials: MA
authors.lastname: de la Garza-Ramos
authors.affiliation: Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Pedro de Alba, s/n, Ciudad Universitaria, 66455 San Nicolas de los Garza, NL, Mexico.
authors.forename: Maria Julia
authors.initials: MJ
authors.lastname: Verde-Star
authors.affiliation: Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Pedro de Alba, s/n, Ciudad Universitaria, 66455 San Nicolas de los Garza, NL, Mexico.
authors.forename: Maria Adriana
authors.initials: MA
authors.lastname: Nuñez-Gonzalez
authors.affiliation: Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Pedro de Alba, s/n, Ciudad Universitaria, 66455 San Nicolas de los Garza, NL, Mexico.
authors.forename: Catalina
authors.identifier: 0000-0002-3626-7263
authors.initials: C
authors.lastname: Leos-Rivas