title: The acute effects of preoperative ozone theraphy on surgical wound healing.
abstract: OBJECTIVE
  To investigate the effects of preoperative rectal ozone insufflation on surgical wound healing over the proinflammatory cytokines and histopathological changes.
  
  
  METHODS
  Twenty one rabbits were divided into 3 groups. Sham, surgical wound, and ozone applied (6 sessions, every other day 70 µg/mL in 12 mL O2-O3 mixture rectally) surgical wound groups were created. TNF-alpha and IL-6 levels from all rabbits were studied at the basal, 24th hour, and 72nd hour. The histopathological examination was done by removing the surgical scar tissue at the end of 72nd hour.
  
  
  RESULTS
  TNF-alfa and IL-6 levels were significantly lower compared to the control group, in the rabbits treated with ozone. The increase in angiogenesis, the decrease in the number of inflammatory cells, epidermal and dermal regeneration, better collagen deposition, and increased keratinisation in stratum corneum were observed in the histopathological examination. It was determined that the wound healing noticeably accelerated in the ozone group.
  
  
  CONCLUSIONS
  Preoperative rectal ozone insufflation had a positive effect on surgical wound healing in acute period.
authors.affiliation: Assistant Professor, Department of Anesthesiology and Reanimation, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. Conception and design of the study; acquisition, analyzis and interpretation of data; manuscript writing; critical revision; final approved.
authors.forename: Hasan
authors.initials: H
authors.lastname: Sahin
authors.affiliation: Assistant Professor, Department of Anesthesiology and Reanimation, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. Conception and design of the study; acquisition, analyzis and interpretation of data; manuscript writing; critical revision; final approved.
authors.forename: Tuncer
authors.initials: T
authors.lastname: Simsek
authors.affiliation: Assistant Professor, Department of Biochemistry, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. of data. Biochemical examinations, final approved.
authors.forename: Hakan
authors.initials: H
authors.lastname: Turkon
authors.affiliation: Associate Professor, Department of Histology and Embryology, Faculty of Medicine, Recep Tayyip Erdoğan University, Rize, Turkey. Histopatological examinations, final approved.
authors.forename: Yıldıray
authors.initials: Y
authors.lastname: Kalkan
authors.affiliation: Assistant Professor, Department of General Surgery, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. Design of the study, acquisition of data, final approved.
authors.forename: Faruk
authors.initials: F
authors.lastname: Ozkul
authors.affiliation: Assistant Professor, Department of Cardiovascular Surgery, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. Design of the study, acquisition and interpretation of data, final approved.
authors.forename: M Turgut Alper
authors.initials: MT
authors.lastname: Ozkan
authors.affiliation: Associate Professor, Department of Anesthesiology and Reanimation, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. Conception and design of the study, technical procedures, acquisition of data, final approved.
authors.forename: Mesut
authors.initials: M
authors.lastname: Erbas
authors.affiliation: Assistant Professor, Department of Anesthesiology and Reanimation, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. Conception and design of the study; acquisition, analyzis and interpretation of data; manuscript writing; critical revision; final approved.
authors.forename: Ugur
authors.initials: U
authors.lastname: Altinisik
authors.affiliation: MD, Department of Anesthesiology and Reanimation, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. Critical revision, final approved.
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Demiraran
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D006097:Granulation Tissue / Q000473:pathology; D007327:Insufflation / Q000379:methods; D015850:Interleukin-6 / Q000378:metabolism*; D010126:Ozone / Q000008:administration & dosage / Q000494:pharmacology*; D011300:Preoperative Care / Q000379:methods; D011817:Rabbits; D000072836:Surgical Wound / Q000188:drug therapy* / Q000473:pathology; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D010126:Ozone