title: A small-angle X-ray scattering study of alpha-synuclein from human red blood cells.
abstract: α-synuclein (α-syn) is the main component of Lewy bodies, which are neuropathological hallmarks of patients with Parkinson's disease. As it has been controversial whether human α-syn from erythrocytes exists as a tetramer under physiological conditions, we tried solving this issue by the small-angle X-ray solution scattering method. Under two different conditions (high ionic strength with a Tris buffer and low ionic strength with an ammonium acetate buffer), no evidence was found for the presence of tetramer. When comparing erythrocyte and recombinant α-syn molecules, we found no significant difference of the molecular weight and the secondary structure although the buffer conditions strongly affect the radius of gyration of the protein. The results indicate that, even though a stable tetramer may not be formed, conformation of α-syn depends much on its environment, which may be the reason for its tendency to aggregate in cells.
authors.affiliation: Department of Neurology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Araki
authors.affiliation: Japan Synchrotron Radiation Research Institute (JASRI), SPring-8, 1-1-1 Kouto, Sayo, Sayo, Hyogo 679-5198, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Yagi
authors.affiliation: Department of Neurology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Nakatani
authors.affiliation: Japan Synchrotron Radiation Research Institute (JASRI), SPring-8, 1-1-1 Kouto, Sayo, Sayo, Hyogo 679-5198, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sekiguchi
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Masatomo
authors.initials: M
authors.lastname: So
authors.affiliation: Center for Research on Green Sustainable Chemistry, Tottori University, 4-101 Koyamacho-minami, Tottori, Tottori 680-8550, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Yagi
authors.affiliation: Japan Synchrotron Radiation Research Institute (JASRI), SPring-8, 1-1-1 Kouto, Sayo, Sayo, Hyogo 679-5198, Japan.
authors.forename: Noboru
authors.initials: N
authors.lastname: Ohta
authors.affiliation: Department of Neurotherapeutics, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nagai
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Goto
authors.affiliation: Department of Neurology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Mochizuki
mesh_terms: D002850:Chromatography, Gel; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D011994:Recombinant Proteins / Q000378:metabolism; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction; D051844:alpha-Synuclein / Q000097:blood*
chemical_list: D011994:Recombinant Proteins; C497604:SNCA protein, human; D051844:alpha-Synuclein