title: Lignin profiling in extracted xylans by size-exclusion chromatography.
abstract: Utilization of the polymeric parts of lignocellulose is expected to gain increasing importance in future biorefinery scenarios. In that respect, a particular focus is placed on hemicelluloses from different wood species gained from an industrially feasible upgrading step in the production of dissolving pulps from paper pulps. During alkaline post-extractions for hemicellulose removal, residual lignins are extracted as well. They are either covalently linked to the extracted hardwood xylans or simply co-dissolved in the alkaline lye. In order to better describe the lignin in xylan containing lyes, a method for lignin profiling was set up by hyphenating size-exclusion chromatography of xylans with UV detection which facilitates visualization of the residual lignin distribution. Simultaneous lignin quantification was achieved with lignin standards prepared from Kraft cooking liquors. The setup presented may serve as advanced characterization for novel xylan products.
authors.affiliation: Kompetenzzentrum Holz GmbH, Altenberger Str. 69, A-4040 Linz, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Hutterer
authors.affiliation: Lenzing AG, Werkstraße 1, A-4860 Lenzing, Austria.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Schild
authors.affiliation: Lenzing AG, Werkstraße 1, A-4860 Lenzing, Austria.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Kliba
authors.affiliation: Universität für Bodenkultur Wien, Department für Chemie, A-1190 Wien, Austria. Electronic address: antje.potthast@boku.ac.at.
authors.forename: Antje
authors.initials: A
authors.lastname: Potthast
mesh_terms: D002850:Chromatography, Gel; D008031:Lignin / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D062065:Wastewater / Q000737:chemistry; D014990:Xylans / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D008031:Lignin; D062065:Wastewater; D014990:Xylans
keywords: Alkaline xylan extraction; Kappa number; Lignin co-extraction; Lignin quantification; Lignin–carbohydrate-linkages; UV measurement