title: An in vitro evaluation of cytotoxicity of curcumin against human dental pulp fibroblasts.
abstract: OBJECTIVE
  The objective of this study was to evaluate the cytotoxicity of curcumin to primary dental pulp fibroblasts in vitro.
  
  
  METHODS
  Dental pulp fibroblasts from primary maxillary central incisors were cultured and used for cytotoxicity tests after the fourth passage. Ninety-five percent curcumin was diluted with dimethylsulfoxide to prepare 100%, 50%, and 25% concentrations. Each concentration of curcumin was added in triplicate into 96-well microtiter plate containing the fibroblast culture at 104/well. Cells without treatment served as a control group. The number of viable cells after 48 hrs incubation at 37°C in a humidified atmosphere of 5 % CO2 and 95 % air was determined by the 3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. The relative viability of pulp cells was expressed as color intensity of the number in the experimental wells relative to that of the control group. Absorbances were read at 492 nm on a microplate reader with a background subtraction at 620 nm.
  
  
  RESULTS
  Cell viability of primary dental pulp fibroblasts to 25%, 50%, and 100% curcumin concentration was 174%, 310%, and 317%, respectively.
  
  
  CONCLUSIONS
  Curcumin promotes cell viability and induces proliferation of primary dental pulp fibroblasts and has the potential to be developed into an economical and reliable medicament for vital pulp therapy.
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India.
authors.forename: Praveenkumar S
authors.initials: PS
authors.lastname: Mandrol
authors.affiliation: Department of Microbiology and Molecular Biology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India.
authors.forename: Kishor
authors.initials: K
authors.lastname: Bhat
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Bapuji Dental College and Hospital, Davangere, Karnataka, India.
authors.forename: A R
authors.initials: AR
authors.lastname: Prabhakar
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003474:Curcumin / Q000633:toxicity*; D003603:Cytotoxins / Q000633:toxicity*; D003782:Dental Pulp / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques
chemical_list: D003603:Cytotoxins; D003474:Curcumin