title: Trans 18-carbon monoenoic fatty acid has distinct effects from its isomeric cis fatty acid on lipotoxicity and gene expression in Saccharomyces cerevisiae.
abstract: Epidemiological studies have suggested that an excess intake of trans-unsaturated fatty acids increases the risk of coronary heart disease. However, the mechanisms of action of trans-unsaturated fatty acids in eukaryotic cells remain unclear. Since the budding yeast Saccharomyces cerevisiae can grow using fatty acids as the sole carbon source, it is a simple and suitable model organism for understanding the effects of trans-unsaturated fatty acids at the molecular and cellular levels. In this study, we compared the physiological effects of Δ9 cis and trans 18-carbon monoenoic fatty acids (oleic acid and elaidic acid) in yeast cells. The results obtained revealed that the two types have distinct effects on the expression of OLE1, which encodes Δ9 desaturase, and lipotoxicity in are1Δare2Δdga1Δlro1Δ and gat1Δ cells. Our results suggest that cis and trans 18-carbon monoenoic fatty acids exert different physiological effects in the regulation of gene expression and processing of excess fatty acids in yeast.
authors.affiliation: Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan.
authors.forename: Toyokazu
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan.
authors.forename: Vo Thi Anh
authors.initials: VT
authors.lastname: Nguyet
authors.affiliation: Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan.
authors.forename: Sae
authors.initials: S
authors.lastname: Kato
authors.affiliation: Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's University, Nishinomiya, Hyogo 663-8558, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Arii
authors.affiliation: Center for Bioresource Field Science, Kyoto Institute of Technology, Sagano, Kyoto 616-8354, Japan.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Akino
authors.affiliation: Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan. Electronic address: thioredoxin@kit.ac.jp.
authors.forename: Shingo
authors.initials: S
authors.lastname: Izawa
mesh_terms: D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D007536:Isomerism; D009154:Mutation; D019301:Oleic Acid / Q000737:chemistry* / Q000633:toxicity*; D009829:Oleic Acids; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase
chemical_list: D009829:Oleic Acids; D019301:Oleic Acid; C011459:elaidic acid; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase
keywords: Elaidic acid; Lipid droplets; Lipotoxicity; Ole1; Oleic acid; Trans-fatty acid