title: A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics.
abstract: Three-dimensional (3D) culture, which can simulate in vivo microenvironments, has been increasingly used to study tumor cell biology. Since most preclinical anti-glioma drug tests still rely on conventional 2D cell culture, we established a collagen scaffold for 3D glioma cell culture. Glioma cells cultured on these 3D scaffolds showed greater degree of dedifferentiation and quiescence than cells in 2D culture. 3D-cultured cells also exhibited enhanced resistance to chemotherapeutic alkylating agents, with a much higher proportion of glioma stem cells and upregulation of O6-methylguanine DNA methyltransferase (MGMT). Importantly, tumor cells in 3D culture showed chemotherapy resistance patterns similar to those observed in glioma patients. Our results suggest that 3D collagen scaffolds are promising in vitro research platforms for screening new anti-glioma therapeutics.
authors.affiliation: Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.|Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, China.
authors.forename: Donglai
authors.initials: D
authors.lastname: Lv
authors.affiliation: Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.|Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, China.
authors.forename: Shi-Cang
authors.initials: SC
authors.lastname: Yu
authors.affiliation: Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.|Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, China.
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Ping
authors.affiliation: Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.|Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Wu
authors.affiliation: Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.|Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, China.
authors.forename: Xilong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.|Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, China.
authors.forename: Huarong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.|Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, China.
authors.forename: Youhong
authors.initials: Y
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, School of Military Preventive Medicine, Third Military Medical University, Chongqing, China.|Institute of Genetics and Development, Chinese Academy of Sciences, Beijing, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.affiliation: Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.|Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, School of Military Preventive Medicine, Third Military Medical University, Chongqing, China.|Institute of Genetics and Development, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
authors.affiliation: Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.|Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, China.
authors.forename: Xiu-Wu
authors.initials: XW
authors.lastname: Bian
authors.affiliation: Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.|Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Yao
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D001932:Brain Neoplasms / Q000188:drug therapy*; D018929:Cell Culture Techniques / Q000379:methods; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D015254:DNA Modification Methylases / Q000378:metabolism; D045643:DNA Repair Enzymes / Q000378:metabolism; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005910:Glioma / Q000188:drug therapy*; D006801:Humans; D007700:Kinetics; D014411:Neoplastic Stem Cells / Q000378:metabolism; D054457:Tissue Scaffolds; D025521:Tumor Suppressor Proteins / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000970:Antineoplastic Agents; D025521:Tumor Suppressor Proteins; D003094:Collagen; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes
keywords: MGMT; chemosensitivity; collagen scaffold; glioma stem cells; three-dimensional culture