title: Humanized Monoclonal Antibody That Passively Protects Mice against Systemic and Intranasal Ricin Toxin Challenge.
abstract: PB10 is a murine monoclonal antibody against an immunodominant epitope on ricin toxin's enzymatic subunit. Here, we characterize a fully humanized version of PB10 IgG1 (hPB10) and demonstrate that it has potent in vitro and in vivo toxin-neutralizing activities. We also report the minimum serum concentrations of hPB10 required to protect mice against 10 times the 50% lethal dose of ricin when delivered by injection and inhalation.
authors.affiliation: Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, New York, USA.
authors.forename: Greta
authors.identifier: http://orcid.org/0000-0003-2646-3030
authors.initials: G
authors.lastname: Van Slyke
authors.affiliation: Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, New York, USA.
authors.forename: Erin K
authors.initials: EK
authors.lastname: Sully
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego, California, USA.
authors.forename: Natasha
authors.initials: N
authors.lastname: Bohorova
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego, California, USA.
authors.forename: Ognian
authors.initials: O
authors.lastname: Bohorov
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego, California, USA.
authors.forename: Do
authors.initials: D
authors.lastname: Kim
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego, California, USA.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Pauly
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego, California, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Whaley
authors.affiliation: Mapp Biopharmaceutical, Inc., San Diego, California, USA larry.zeitlin@mappbio.com nicholas.mantis@health.ny.gov.
authors.forename: Larry
authors.initials: L
authors.lastname: Zeitlin
authors.affiliation: Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, New York, USA Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, New York, USA larry.zeitlin@mappbio.com nicholas.mantis@health.ny.gov.
authors.forename: Nicholas J
authors.identifier: http://orcid.org/0000-0002-5083-8640
authors.initials: NJ
authors.lastname: Mantis
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000302:isolation & purification / Q000494:pharmacology; D061067:Antibodies, Monoclonal, Humanized / Q000008:administration & dosage* / Q000302:isolation & purification / Q000494:pharmacology; D000992:Antitoxins / Q000008:administration & dosage* / Q000302:isolation & purification / Q000494:pharmacology; D002619:Chemical Warfare Agents / Q000633:toxicity*; D004195:Disease Models, Animal; D005260:Female; D008807:Mice, Inbred BALB C; D011041:Poisoning / Q000628:therapy*; D012276:Ricin / Q000633:toxicity*; D016019:Survival Analysis; D016896:Treatment Outcome
chemical_list: D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D000992:Antitoxins; D002619:Chemical Warfare Agents; D012276:Ricin