title: Chemical Constituents of Mangifera indica and Their Antiausterity Activity against the PANC-1 Human Pancreatic Cancer Cell Line.
abstract: Human pancreatic cancer cell lines such as PANC-1 have an altered metabolism, enabiling them to tolerate and survive under extreme conditions of nutrient starvation. The search for candidates that inhibit their viability during nutrition starvation represents a novel antiausterity strategy in anticancer drug discovery. A methanol extract of the bark of Mangifera indica was found to inhibit the survival of PANC-1 human pancreatic cancer cells preferentially under nutrient-deprived conditions with a PC50 value of 15.5 μg/mL, without apparent toxicity, in normal nutrient-rich conditions. Chemical investigation on this bioactive extract led to the isolation of 19 compounds (1-19), including two new cycloartane-type triterpenes, mangiferolate A (1) and mangiferolate B (2). The structures of 1 and 2 were determined by NMR spectroscopic analysis. Among the isolated compounds, mangiferolate B (2) and isoambolic acid (12) exhibited potent preferential cytotoxicity against PANC-1 human pancreatic cancer cells under the nutrition-deprived condition with PC50 values of 11.0 and 4.8 μM, respectively.
authors.affiliation: Faculty of Chemistry, University of Science, Vietnam National University-Hochiminh City , 227 Nguyen Van Cu Street, District 5, Vietnam.
authors.forename: Hai Xuan
authors.initials: HX
authors.lastname: Nguyen
authors.affiliation: Faculty of Chemistry, University of Science, Vietnam National University-Hochiminh City , 227 Nguyen Van Cu Street, District 5, Vietnam.
authors.forename: Truong Nhat Van
authors.initials: TN
authors.lastname: Do
authors.affiliation: Faculty of Chemistry, University of Science, Vietnam National University-Hochiminh City , 227 Nguyen Van Cu Street, District 5, Vietnam.
authors.forename: Tho Huu
authors.initials: TH
authors.lastname: Le
authors.affiliation: Faculty of Chemistry, University of Science, Vietnam National University-Hochiminh City , 227 Nguyen Van Cu Street, District 5, Vietnam.
authors.forename: Mai Thanh Thi
authors.initials: MT
authors.lastname: Nguyen
authors.affiliation: Faculty of Chemistry, University of Science, Vietnam National University-Hochiminh City , 227 Nguyen Van Cu Street, District 5, Vietnam.
authors.forename: Nhan Trung
authors.initials: NT
authors.lastname: Nguyen
authors.affiliation: Research Institute for Biomedical Sciences, Tokyo University of Science , Chiba 278-8510, Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Esumi
authors.affiliation: Division of Natural Drug Discovery, Department of Translational Research, Institute of Natural Medicine, University of Toyama , 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Suresh
authors.initials: S
authors.lastname: Awale
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D031022:Mangifera / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010190:Pancreatic Neoplasms / Q000188:drug therapy*; D024301:Plant Bark / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014744:Vietnam
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes; C000617758:mangiferolate A; C000617759:mangiferolate B