title: Boiling Method-Based Zinc Oxide Nanorods for Enhancement of Adipose-Derived Stem Cell Proliferation.
abstract: Adipose-derived stem cells (ASCs) are typically expanded to acquire large numbers of cells for therapeutic applications. Diverse stimuli such as sphingosylphosphocholine and vitamin C have been used to increase the production yield and regenerative potential of ASCs. In the present study, we hypothesized that ZnO nanorods have promising potential for the enhancement of ASC proliferation. ZnO nanorods were prepared using three different methods: grinding and boiling at low temperature with and without surfactant. The physicochemical properties of the nanorods such as their crystallinity, morphology, size, and solvent compatibility were evaluated, and then, the ability of the synthesized ZnO nanorods to enhance ASC proliferation was investigated. Scanning electron microscopy images of all of the ZnO powders showed rod-shaped nanoflakes with lengths of 200-500 nm. Notably, although ZnO-G produced by the grinding method was well dispersed in ethanol, atomic force microscopy images of dispersions of both ZnO-B from boiling methods and ZnO-G indicated the presence of clusters of ZnO nanorods. In contrast, ZnO-B was freely dispersible in 5% dextrose of water and dimethyl sulfoxide, whereas ZnO-G and ZnO-M, produced by boiling with ethanolamine, were not. All three types of ZnO nanorods increased the proliferation of ASCs in a dose-dependent manner. These results collectively suggest that ZnO nanorods have promising potential for use as an agent for the enhancement of ASC proliferation.
authors.affiliation: 1 College of Pharmacy, Yonsei University , Incheon, Korea.|2 College of Medicine, Yonsei University , Seoul, Korea.|3 Institutes of Pharmaceutical Sciences, Yonsei University , Incheon, Korea.
authors.forename: Su-Eon
authors.initials: SE
authors.lastname: Jin
authors.affiliation: 1 College of Pharmacy, Yonsei University , Incheon, Korea.
authors.forename: Hyo-Sun
authors.initials: HS
authors.lastname: Ahn
authors.affiliation: 1 College of Pharmacy, Yonsei University , Incheon, Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Kim
authors.affiliation: 4 Department of Biomedical Science, CHA University , Seongnam, Korea.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Arai
authors.affiliation: 4 Department of Biomedical Science, CHA University , Seongnam, Korea.
authors.forename: Soo-Hong
authors.initials: SH
authors.lastname: Lee
authors.affiliation: 5 College of Pharmacy, Ajou University , Suwon, Korea.
authors.forename: Tae-Jong
authors.initials: TJ
authors.lastname: Yoon
authors.affiliation: 1 College of Pharmacy, Yonsei University , Incheon, Korea.|3 Institutes of Pharmaceutical Sciences, Yonsei University , Incheon, Korea.
authors.forename: Sung-Joo
authors.initials: SJ
authors.lastname: Hwang
authors.affiliation: 1 College of Pharmacy, Yonsei University , Incheon, Korea.|3 Institutes of Pharmaceutical Sciences, Yonsei University , Incheon, Korea.|6 Stemmore, Co. Ltd. , Incheon, Korea.
authors.forename: Jong-Hyuk
authors.initials: JH
authors.lastname: Sung
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D036103:Nanotechnology / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology*; D013499:Surface Properties; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D015034:Zinc Oxide