title: A novel and simple fluorescence probe for detecting main group magnesium ion in HeLa cells and Arabidopsis.
abstract: A simple-molecule fluorescence probe L has been designed, synthesized and characterized, which shows high selectivity and sensitivity for the main group magnesium ion through fluorescence "turn-on" response in ethanol solution, and no interference from calcium ion in particular. Detection limit of probe L is 1.47×10(-6) M and the rapid response could reach about 15-20s. The recognition mechanism has been established by fluorescence spectra, (1)H NMR study. Moreover, probe L presents a great photostability, low toxicity and cellular permeability, then we have carried out fluorescent bio-imaging of the probe L for magnesium ions in HeLa cells, which showed that probe L could be utilized to detect the intracellular magnesium ion. Furthermore, it is successfully used as a magnesium ion developer in plant tissues, which shows that it not only can be well tracking the transport of magnesium ion but also make a corresponding fluorescence response to different concentrations magnesium ion. These results would make this probe a great potential application for detecting Mg(2+) in biological system.
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, Hefei, 230601 PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Yu
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601 PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Sun
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, Hefei, 230601 PR China.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, Hefei, 230601 PR China.
authors.forename: Yinggang
authors.initials: Y
authors.lastname: Ji
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, Hefei, 230601 PR China. Electronic address: zhpzhp@263.net.
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhou
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601 PR China.
authors.forename: Baowei
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, Hefei, 230601 PR China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Tian
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, Hefei, 230601 PR China.
authors.forename: Jieying
authors.initials: J
authors.lastname: Wu
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D008274:Magnesium / Q000032:analysis / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013347:Subcellular Fractions / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D008274:Magnesium
keywords: Animal cells; Biological application; Fluorescence probe; Main group magnesium ion; Plant tissues; Recognition mechanism