title: Polyhydroquinone-graphene composite as new redox species for sensitive electrochemical detection of cytokeratins antigen 21-1.
abstract: Polyhydroquinone-graphene composite as a new redox species was synthesized simply by a microwave-assisted one-pot method through oxidative polymerization of hydroquinone by graphene oxide, which exhibited excellent electrochemical redox activity at 0.124 V and can remarkably promote electron transfer. The as-prepared composite was used as immunosensing substrate in a label-free electrochemical immunosensor for the detection of cytokeratins antigen 21-1, a kind of biomarker of lung cancer. The proposed immunosensor showed wide liner range from 10 pg mL(-1) to 200 ng mL(-1) with a detection limit 2.3 pg mL(-1), and displayed a good stability and selectivity. In addition, this method has been used for the analysis of human serum sample, and the detection results showed good consistence with those of ELISA. The present substrate can be easily extended to other polymer-based nanocomposites.
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing, 100048, China.
authors.forename: Huiqiang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing, 100048, China.
authors.forename: Qinfeng
authors.initials: Q
authors.lastname: Rong
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing, 100048, China.
authors.forename: Zhanfang
authors.initials: Z
authors.lastname: Ma
mesh_terms: D000941:Antigens / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D003188:Composite Resins / Q000138:chemical synthesis / Q000737:chemistry*; D055664:Electrochemical Techniques; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007633:Keratins / Q000032:analysis*; D008175:Lung Neoplasms / Q000175:diagnosis; D010084:Oxidation-Reduction; D011809:Quinones / Q000737:chemistry*
chemical_list: D000941:Antigens; D014408:Biomarkers, Tumor; D003188:Composite Resins; D011809:Quinones; D007633:Keratins; D006108:Graphite