title: Butanol production in acetone-butanol-ethanol fermentation with in situ product recovery by adsorption.
abstract: Activated carbon Norit ROW 0.8, zeolite CBV901, and polymeric resins Dowex Optipore L-493 and SD-2 with high specific loadings and partition coefficients were studied for n-butanol adsorption. Adsorption isotherms were found to follow Langmuir model, which can be used to estimate the amount of butanol adsorbed in acetone-butanol-ethanol (ABE) fermentation. In serum-bottle fermentation with in situ adsorption, activated carbon showed the best performance with 21.9g/L of butanol production. When operated in a fermentor, free- and immobilized-cell fermentations with adsorption produced 31.6g/L and 54.6g/L butanol with productivities of 0.30g/L·h and 0.45g/L·h, respectively. Thermal desorption produced a condensate containing ∼167g/L butanol, which resulted in a highly concentrated butanol solution of ∼640g/L after spontaneous phase separation. This in situ product recovery process with activated carbon is energy efficient and can be easily integrated with ABE fermentation for n-butanol production.
authors.affiliation: Department of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China; Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
authors.forename: Chuang
authors.initials: C
authors.lastname: Xue
authors.affiliation: Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Xu
authors.affiliation: Bioprocessing Innovative Company, 4734 Bridle Path Court, Dublin, OH 43017, USA.
authors.forename: I-Ching
authors.initials: IC
authors.lastname: Tang
authors.affiliation: Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Fengwu
authors.initials: F
authors.lastname: Bai
authors.affiliation: Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA. Electronic address: yang.15@osu.edu.
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
mesh_terms: D020001:1-Butanol / Q000737:chemistry*; D000096:Acetone; D000327:Adsorption; D000431:Ethanol; D005285:Fermentation; D012117:Resins, Synthetic / Q000737:chemistry*; D017641:Zeolites / Q000737:chemistry*
chemical_list: D012117:Resins, Synthetic; D017641:Zeolites; D000096:Acetone; C027972:Dowex; D000431:Ethanol; D020001:1-Butanol
keywords: Acetone-butanol-ethanol fermentation; Activated carbon; Adsorption; Butanol; In situ product recovery