title: hTERT promoter methylation in meningiomas and central nervous hemangiopericytomas.
abstract: In meningiomas, prognostic impact of mutations in the human telomerase reverse transcriptase (hTERT) promoter region was recently shown, while studies of promoter methylation and analyses of hemangiopericytomas are lacking. hTERT promoter methylation was analyzed in 78 meningioma and 38 meningeal hemangiopericytoma samples by methylation-specific polymerase chain reaction (MS-PCR) and compared with histopathological and clinical variables and with immunohistochemical hTERT expression. Promoter methylation was found in 62 samples (53 %) and tended to be higher in meningiomas (N = 19/41, 46 %) than in hemangiopericytomas (N = 8/33, 24 %, p = .057). In meningiomas, methylation was 16, 60 and 77 % in grade I, II and III tumors (p < .001) and higher in recurrent (N = 33/37, 89 %) than in primary diagnosed (N = 19/41, 46 %) tumors (OR 5.14, 95 % CI 1.34-19.71, p = .017). Univariate analyses showed shorter mean progression free and overall survival in methylated than in unmethylated individuals (26 vs. 100 months; p = .045 and 110 vs. 113 months; p = .025, respectively). Moreover, hTERT expression was found in 70 % (N = 53) and was more frequent in methylated than in unmethylated samples (78 vs. 52 %, OR 3.36, 95 % CI 1.20-9.40, p = .021). In hemangiopericytomas, methylation was similar in grade II (24 %) and III (25 %, p > .05) and in primary (24 %) and recurrent tumors (40 %, p > .05). hTERT expression was similar as compared to meningiomas (74 %, N = 28, p > .05) but was independent of promoter methylation (OR 4.26, 95 % CI 0.47-39.0, p = .199). In meningeal tumors, hTERT promoter methylation is more common than mutations and in meningiomas but not in hemangiopericytomas positively correlated with WHO grade and hTERT expression.
authors.affiliation: Department of Neurosurgery, University Hospital Münster, Albert-Schweitzer-Campus 1, Building A1, 48149, Münster, Germany.
authors.forename: Gina
authors.initials: G
authors.lastname: Fürtjes
authors.affiliation: Department of Neurosurgery, University Hospital Münster, Albert-Schweitzer-Campus 1, Building A1, 48149, Münster, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Köchling
authors.affiliation: Institute of Neuropathology, University Hospital Münster, Pottkamp 2, 48149, Münster, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Peetz-Dienhart
authors.affiliation: Institute of Neuropathology, University Hospital Münster, Pottkamp 2, 48149, Münster, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Wagner
authors.affiliation: Institute of Neuropathology, University Hospital Münster, Pottkamp 2, 48149, Münster, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Heß
authors.affiliation: Institute of Neuropathology, University Hospital Münster, Pottkamp 2, 48149, Münster, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Hasselblatt
authors.affiliation: Institute of Neuropathology, University Hospital Münster, Pottkamp 2, 48149, Münster, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Senner
authors.affiliation: Department of Neurosurgery, University Hospital Münster, Albert-Schweitzer-Campus 1, Building A1, 48149, Münster, Germany.
authors.forename: Walter
authors.initials: W
authors.lastname: Stummer
authors.affiliation: Institute of Neuropathology, University Hospital Münster, Pottkamp 2, 48149, Münster, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Paulus
authors.affiliation: Department of Neurosurgery, University Hospital Münster, Albert-Schweitzer-Campus 1, Building A1, 48149, Münster, Germany. benjamin.brokinkel@ukmuenster.de.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Brokinkel
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016543:Central Nervous System Neoplasms / Q000235:genetics* / Q000401:mortality; D019175:DNA Methylation; D005260:Female; D005500:Follow-Up Studies; D006393:Hemangiopericytoma / Q000235:genetics* / Q000401:mortality; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008579:Meningioma / Q000235:genetics* / Q000401:mortality; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*
chemical_list: C509186:TERT protein, human; D019098:Telomerase
keywords: Meningeal hemangiopericytoma; Meningioma; Methylation; Promoter; TERT