title: Tescalcin expression contributes to invasive and metastatic activity in colorectal cancer.
abstract: We reported previously that tescalcin (TESC) levels were higher in tissue and serum from colorectal cancer (CRC) patients and suggested that TESC was a potential oncotarget in CRC. The aim of this study was to investigate the function of TESC in CRC invasion and metastatic potential. TESC expression was knocked down in CRC cells using small interfering RNA (siRNA). The expression of TESC siRNA reduced cell migration and invasion by inhibiting matrix metalloprotease (MMP) and the epithelial-mesenchymal transition (EMT) pathway. RT-PCR and Western blot analysis showed that TESC siRNA induced E-cadherin. Consistently, TESC overexpression in HCT116 (HCT/TESC) cells enhanced cell migration and invasion by activating MMP and the EMT pathway and reducing E-cadherin. The formation of liver metastatic nodules in vivo was strongly increased in mice injected with HCT/TESC cells compared with that in mice injected with HCT/mock cells. This study demonstrates that TESC is involved in cell migration, invasion, and EMT during CRC tumor invasion. These results implicate TESC as a metastatic mediator and provide a biological rationale for the adverse prognosis associated with elevated TESC expression in human CRC.
authors.affiliation: Genome Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.
authors.forename: Jieun
authors.initials: J
authors.lastname: Kang
authors.affiliation: Genome Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.|Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk, South Korea.
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Kang
authors.affiliation: Genome Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.|Department of Biomolecular Science, University of Science and Technology (UST), Daejeon, South Korea.
authors.forename: Byung Moo
authors.initials: BM
authors.lastname: Oh
authors.affiliation: Genome Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.
authors.forename: Tae Gi
authors.initials: TG
authors.lastname: Uhm
authors.affiliation: Genome Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.|Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk, South Korea.
authors.forename: Sang Yoon
authors.initials: SY
authors.lastname: Park
authors.affiliation: Genome Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.|Department of Biomolecular Science, University of Science and Technology (UST), Daejeon, South Korea.
authors.forename: Tae Woo
authors.initials: TW
authors.lastname: Kim
authors.affiliation: Department of Biological Science, Daejeon University, Daejeon, South Korea.
authors.forename: Seung Ro
authors.initials: SR
authors.lastname: Han
authors.affiliation: Genome Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.|Department of Biomolecular Science, University of Science and Technology (UST), Daejeon, South Korea.
authors.forename: Seon-Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Genome Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.|Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk, South Korea.
authors.forename: Younghee
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Genome Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea. hglee@kribb.re.kr.|Department of Biomolecular Science, University of Science and Technology (UST), Daejeon, South Korea. hglee@kribb.re.kr.
authors.forename: Hee Gu
authors.initials: HG
authors.lastname: Lee
mesh_terms: D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary* / Q000601:surgery; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; C486009:TESC protein, human
keywords: Colorectal cancer; Epithelial-mesenchymal transition; Invasion; Metastatic mediator; Tescalcin