title: Polyaromatic hydrocarbon (PAH) degradation potential of a new acid tolerant, diazotrophic P-solubilizing and heavy metal resistant bacterium Cupriavidus sp. MTS-7 isolated from long-term mixed contaminated soil.
abstract: An isolate of Cupriavidus (strain MTS-7) was identified from a long-term PAHs and heavy metals mixed contaminated soil with the potential to biodegrade both LMW and HMW PAHs with added unique traits of acid and alkali tolerance, heavy metal tolerance, self-nutrient assimilation by N fixation and P solubilization. This strain completely degraded the model 3 (150 mg L(-1) Phe), 4 (150 mg L(-1) Pyr) and 5 (50 mg L(-1) BaP) ring PAHs in 4, 20 and 30 days, respectively. It could mineralize 90-100% of PAHs (200 mg L(-1) of Phe and Pyr) within 15 days across pH ranging from 5 to 8 and even in the presence of toxic metal contaminations. During biodegradation, the minimum inhibitory concentrations were 5 (Cu(2+)) and 3 (Cd(2+), Pb(2+), Zn(2+)) mg L(-1) of the potentially bioavailable metal ions and over 17 mg L(-1) metal levels was lethal for the microbe. Further, it could fix 217-274 μg mL(-1) of N and solubilize 79-135 μg mL(-1) of P while PAHs degradation. MTS-7 as a superior candidate could be thus used in the enhanced bioaugmentation and/or phytoremediation of long-term mixed contaminated sites.
authors.affiliation: Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660-701, South Korea; Centre for Environmental Risk Assessment and Remediation (CERAR), University of South Australia, Mawson Lakes, SA5095, Australia; Cooperative Research Centre for Contamination Assessment and Remediation of Environment (CRC CARE), PO Box 486, Salisbury South, SA5106, Australia. Electronic address: saran.miles2go@gmail.com.
authors.forename: Saranya
authors.initials: S
authors.lastname: Kuppusamy
authors.affiliation: Cooperative Research Centre for Contamination Assessment and Remediation of Environment (CRC CARE), PO Box 486, Salisbury South, SA5106, Australia; Global Centre for Environmental Remediation (GCER), Faculty of Science and Information Technology, The University of Newcastle, Callaghan, NSW, 2308, Australia.
authors.forename: Palanisami
authors.initials: P
authors.lastname: Thavamani
authors.affiliation: Centre for Environmental Risk Assessment and Remediation (CERAR), University of South Australia, Mawson Lakes, SA5095, Australia; Cooperative Research Centre for Contamination Assessment and Remediation of Environment (CRC CARE), PO Box 486, Salisbury South, SA5106, Australia; Global Centre for Environmental Remediation (GCER), Faculty of Science and Information Technology, The University of Newcastle, Callaghan, NSW, 2308, Australia.
authors.forename: Mallavarapu
authors.initials: M
authors.lastname: Megharaj
authors.affiliation: Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660-701, South Korea.
authors.forename: Yong Bok
authors.initials: YB
authors.lastname: Lee
authors.affiliation: Centre for Environmental Risk Assessment and Remediation (CERAR), University of South Australia, Mawson Lakes, SA5095, Australia; Cooperative Research Centre for Contamination Assessment and Remediation of Environment (CRC CARE), PO Box 486, Salisbury South, SA5106, Australia; Global Centre for Environmental Remediation (GCER), Faculty of Science and Information Technology, The University of Newcastle, Callaghan, NSW, 2308, Australia.
authors.forename: Ravi
authors.initials: R
authors.lastname: Naidu
mesh_terms: D000222:Adaptation, Physiological; D001673:Biodegradation, Environmental; D049921:Cupriavidus / Q000502:physiology*; D019216:Metals, Heavy / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D012987:Soil; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
chemical_list: D019216:Metals, Heavy; D011084:Polycyclic Aromatic Hydrocarbons; D012987:Soil; D012989:Soil Pollutants
keywords: Acid-tolerant bacteria; Heavy metal resistance; N-fixation; P-solubilization; PAH degradation