title: In Vivo Whole-Cell Patch-Clamp Recording in the Zebrafish Brain.
abstract: Zebrafish (Danio rerio) is a newly emerged vertebrate animal model with a conserved gross architecture of the brain and a rich repertoire of behaviors. Due to the optical transparency and structural simplicity of its brain, larval zebrafish has become an ideal in vivo model for dissecting neural mechanisms of brain functions at a whole-brain scale based on a strategy that spans scales from synapses, neurons, and circuits to behaviors. Whole-cell patch-clamp recording is an indispensable approach for studying synaptic and circuit mechanisms of brain functions. Due to the small size of neurons in the zebrafish brain, it is challenging to get whole-cell recordings from these cells. Here, we describe a protocol for obtaining in vivo whole-cell patch-clamp recordings from neurons in larval zebrafish.
authors.affiliation: Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai, 200031, China. zhangrw@ion.ac.cn.
authors.forename: Rong-Wei
authors.initials: RW
authors.lastname: Zhang
authors.affiliation: Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai, 200031, China. forestdu@ion.ac.cn.|Graduate School, University of Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai, 200031, China. forestdu@ion.ac.cn.|School of Life Science and Technology, ShanghaiTech University, 319 Yue-Yang Road, Shanghai, 200031, China. forestdu@ion.ac.cn.
authors.forename: Jiu-Lin
authors.initials: JL
authors.lastname: Du
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology* / Q000502:physiology*; D007814:Larva / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods*; D013569:Synapses / Q000378:metabolism / Q000502:physiology; D015027:Zebrafish
keywords: Neuron; Perforated patch-clamp recording; Whole-cell patch-clamp recording; Zebrafish larvae