title: miR-22-5p revealed as a potential biomarker involved in the acute phase of myocardial infarction via profiling of circulating microRNAs.
abstract: Acute myocardial infarction (AMI) is a life-threatening episode of coronary artery disease. Recently, circulating myocardial-derived microRNAs (miRNAs) have been reported as potential biomarkers of infarction. The present study aimed to identify differentially expressed miRNAs in patients with ST-segment elevation myocardial infarction that could be potentially dysregulated in response to early myocardial damage. miRNA expression profile analysis was performed using the Serum/Plasma Focus miRNA Polymerase Chain Reaction (PCR) panel of Exiqon A/S (Vedbaek, Denmark) on plasma samples of patients on the first day of AMI (admission) and on samples from the identical patients collected six months following AMI. Selected miRNAs were validated by reverse transcription‑quantitative PCR (RT‑qPCR) using independent patients with AMI and a control group of patients with a stable coronary artery disease. Thirty‑two species of plasma miRNA were differentially expressed (P<0.05) on admission compared with six months following AMI. Subsequent validation in an independent patient group confirmed that miR‑133b and miR‑22‑5p were significantly up‑regulated in the serum of patients with AMI. The receiver operating characteristic (ROC) curve analysis demonstrated a diagnostic utility for miR-22-5p, which has not previously been reported to be associated with AMI. Among the selected miRNAs, miR‑22‑5p represents a novel promising biomarker for the diagnosis of AMI.
authors.affiliation: Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02‑106 Warsaw, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Maciejak
authors.affiliation: Department of Cardiology and Internal Diseases, Military Institute of Medicine, 04‑141 Warsaw, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Kiliszek
authors.affiliation: 1st Chair and Department of Cardiology, Medical University of Warsaw, 02‑091 Warsaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Opolski
authors.affiliation: 1st Faculty of Medicine, Medical University of Warsaw, 02‑091 Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Segiet
authors.affiliation: Department of Cardiac Surgery, Medical University of Bialystok, 15‑089 Bialystok, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Matlak
authors.affiliation: Department of Invasive Cardiology, Medical University of Bialystok, 15‑089 Bialystok, Poland.
authors.forename: Slawomir
authors.initials: S
authors.lastname: Dobrzycki
authors.affiliation: Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02‑106 Warsaw, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Tulacz
authors.affiliation: Department of Medical Laboratory Diagnostics, Medical University of Warsaw, 02‑091 Warsaw, Poland.
authors.forename: Grazyna
authors.initials: G
authors.lastname: Sygitowicz
authors.affiliation: Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02‑106 Warsaw, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Burzynska
authors.affiliation: Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02‑106 Warsaw, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Gora
mesh_terms: D000328:Adult; D015415:Biomarkers; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D012372:ROC Curve; D015203:Reproducibility of Results; D012307:Risk Factors
chemical_list: D015415:Biomarkers; C548375:MIRN22 microRNA, human; D035683:MicroRNAs