title: Quantitative determination of multi markers in five varieties of Withania somnifera using ultra-high performance liquid chromatography with hybrid triple quadrupole linear ion trap mass spectrometer combined with multivariate analysis: Application to pharmaceutical dosage forms.
abstract: An ultra-high performance liquid chromatography electrospray ionization tandem mass spectrometry method has been developed and validated for simultaneous quantification of six major bioactive compounds in five varieties of Withania somnifera in various plant parts (leaf, stem and root). The analysis was accomplished on Waters ACQUITY UPLC BEH C18 column with linear gradient elution of water/formic acid (0.1%) and acetonitrile at a flow rate of 0.3mLmin(-1). The proposed method was validated with acceptable linearity (r(2), 0.9989-0.9998), precision (RSD, 0.16-2.01%), stability (RSD, 1.04-1.62%) and recovery (RSD ≤2.45%), under optimum conditions. The method was also successfully applied for the simultaneous determination of six marker compounds in twenty-six marketed formulations. Hierarchical cluster analysis and principal component analysis were applied to discriminate these twenty-six batches based on characteristics of the bioactive compounds. The results indicated that this method is advance, rapid, sensitive and suitable to reveal the quality of Withania somnifera and also capable of performing quality evaluation of polyherbal formulations having similar markers/raw herbs.
authors.affiliation: Professor (AcSIR) & Senior Principal Scientist, Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow 226031, Uttar Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi 110025, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Chandra
authors.affiliation: Soil Science Division, CSIR-National Botanical Research Institute, Lucknow 226001, Uttar Pradesh, India.
authors.forename: Rekha
authors.initials: R
authors.lastname: Kannujia
authors.affiliation: Phytochemistry Division, CSIR-National Botanical Research Institute, Lucknow 226001, Uttar Pradesh, India.
authors.forename: Ankita
authors.initials: A
authors.lastname: Saxena
authors.affiliation: Biometry and Statistics Division, CSIR-Central Drug Research Institute, Lucknow 226031, Uttar Pradesh, India.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Srivastava
authors.affiliation: Soil Science Division, CSIR-National Botanical Research Institute, Lucknow 226001, Uttar Pradesh, India.
authors.forename: Lal
authors.initials: L
authors.lastname: Bahadur
authors.affiliation: Phytochemistry Division, CSIR-National Botanical Research Institute, Lucknow 226001, Uttar Pradesh, India.
authors.forename: Mahesh
authors.initials: M
authors.lastname: Pal
authors.affiliation: Department of Biotechnology, Mizoram University, Tanharil, Aizawl, Mizoram 796004, India.
authors.forename: Bhim Pratap
authors.initials: BP
authors.lastname: Singh
authors.affiliation: Ethnopharmacology Division, National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Kumar Ojha
authors.affiliation: Professor (AcSIR) & Senior Principal Scientist, Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow 226031, Uttar Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi 110025, India. Electronic address: brijesh_kumar@cdri.res.in.
authors.forename: Brijesh
authors.initials: B
authors.lastname: Kumar
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004304:Dosage Forms; D057230:Limit of Detection; D015999:Multivariate Analysis; D018515:Plant Leaves / Q000737:chemistry; D028321:Plant Preparations / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D025341:Principal Component Analysis / Q000379:methods; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods; D032341:Withania / Q000737:chemistry*
chemical_list: D004304:Dosage Forms; D028321:Plant Preparations
keywords: Hierarchical cluster analysis; Marker quantification; Marketed formulation; Multiple reaction monitoring; Principal component analysis; UHPLC–ESI-MS/MS