title: Investigation of septins using infection by bacterial pathogens.
abstract: Investigation of the host cytoskeleton during infection by bacterial pathogens has significantly contributed to our understanding of cell biology and host defense. Work has shown that septins are recruited to the phagocytic cup as collarlike structures and enable bacterial entry into host cells. In the cytosol, septins can entrap actin-polymerizing bacteria in cage-like structures for targeting to autophagy, a highly conserved intracellular degradation process. In this chapter, we describe methods to investigate septin assembly and function during infection by bacterial pathogens. Use of these methods can lead to in-depth understanding of septin biology and suggest therapeutic approaches to combat infectious disease.
authors.affiliation: Imperial College London, London, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Krokowski
authors.affiliation: Imperial College London, London, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Mostowy
mesh_terms: D000199:Actins / Q000737:chemistry; D001343:Autophagy / Q000235:genetics*; D002462:Cell Membrane / Q000382:microbiology / Q000473:pathology; D003599:Cytoskeleton / Q000737:chemistry / Q000235:genetics*; D003600:Cytosol / Q000382:microbiology / Q000473:pathology; D004405:Dysentery, Bacillary / Q000235:genetics* / Q000382:microbiology; D006367:HeLa Cells; D006801:Humans; D058112:Septins / Q000737:chemistry / Q000235:genetics*; D012760:Shigella / Q000235:genetics / Q000472:pathogenicity
chemical_list: D000199:Actins; D058112:Septins
keywords: Actin; Autophagy; Cellular microbiology; Cytoskeleton; Septin; Shigella