title: Tissue-based quantitative proteome analysis of human hepatocellular carcinoma using tandem mass tags.
abstract: OBJECTIVE
  Human hepatocellular carcinoma (HCC) is a severe malignant disease, and accurate and reliable diagnostic markers are still needed. This study was aimed for the discovery of novel marker candidates by quantitative proteomics.
  
  
  RESULTS
  Proteomic differences between HCC and nontumorous liver tissue were studied by mass spectrometry. Among several significantly upregulated proteins, translocator protein 18 (TSPO) and Ras-related protein Rab-1A (RAB1A) were selected for verification by immunohistochemistry in an independent cohort. For RAB1A, a high accuracy for the discrimination of HCC and nontumorous liver tissue was observed.
  
  
  CONCLUSIONS
  RAB1A was verified to be a potent biomarker candidate for HCC.
authors.affiliation: a Medizinisches Proteom-Center, Ruhr-Universität Bochum , Germany.
authors.forename: Dominik Andre
authors.initials: DA
authors.lastname: Megger
authors.affiliation: a Medizinisches Proteom-Center, Ruhr-Universität Bochum , Germany.
authors.forename: Kristin
authors.initials: K
authors.lastname: Rosowski
authors.affiliation: a Medizinisches Proteom-Center, Ruhr-Universität Bochum , Germany.
authors.forename: Maike
authors.initials: M
authors.lastname: Ahrens
authors.affiliation: a Medizinisches Proteom-Center, Ruhr-Universität Bochum , Germany.
authors.forename: Thilo
authors.initials: T
authors.lastname: Bracht
authors.affiliation: a Medizinisches Proteom-Center, Ruhr-Universität Bochum , Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Eisenacher
authors.affiliation: b Department of Gastroenterology and Hepatology , University Hospital of Essen , Germany.
authors.forename: Jörg F
authors.initials: JF
authors.lastname: Schlaak
authors.affiliation: c Department of General, Visceral and Transplantation Surgery , University Hospital of Essen , Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Weber
authors.affiliation: d Department of Medicine (Cancer Research), Molecular Oncology Risk-Profile Evaluation , University Hospital of Essen , Germany.
authors.forename: Andreas-Claudius
authors.initials: AC
authors.lastname: Hoffmann
authors.affiliation: a Medizinisches Proteom-Center, Ruhr-Universität Bochum , Germany.
authors.forename: Helmut E
authors.initials: HE
authors.lastname: Meyer
authors.affiliation: e Department of Pathology , University Hospital of Essen , Germany.
authors.forename: Hideo A
authors.initials: HA
authors.lastname: Baba
authors.affiliation: a Medizinisches Proteom-Center, Ruhr-Universität Bochum , Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Sitek
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis*; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation; D020761:rab1 GTP-Binding Proteins / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D020543:Proteome; D020761:rab1 GTP-Binding Proteins
keywords: Proteomics; RAB1A; TSPO; immunohistochemistry; liver cancer; tandem mass tags