title: Effects of carbon dioxide on juveniles of the freshwater mussel (Lampsilis siliquoidea [Unionidae]).
abstract: Carbon dioxide (CO2) has shown promise as a tool to control movements of invasive Asian carp, but its effects on native freshwater biota have not been well studied. The authors evaluated lethal and sublethal responses of juvenile fatmucket (Lampsilis siliquoidea) mussels to CO2at levels (43-269 mg/L, mean concentration) that bracket concentrations effective for deterring carp movement. The 28-d lethal concentration to 50% of the mussels was 87.0 mg/L (95% confidence interval [CI] 78.4-95.9) and at 16-d postexposure, 76.0 mg/L (95% CI 62.9-90.3). A proportional hazards regression model predicted that juveniles could not survive CO2concentrations >160 mg/L for more than 2 wk or >100 mg/L CO2for more than 30 d. Mean shell growth was significantly lower for mussels that survived CO2treatments. Growth during the postexposure period did not differ among treatments, indicating recovery of the mussels. Also, CO2caused shell pitting and erosion. Behavioral effects of CO2included movement of mussels to the substrate surface and narcotization at the highest concentrations. Mussels in the 110 mg/L mean CO2treatment had the most movements in the first 3 d of exposure. If CO2is infused continuously as a fish deterrent, concentrations <76 mg/L are recommended to prevent juvenile mussel mortality and shell damage. Mussels may survive and recover from brief exposure to higher concentrations. Environ Toxicol Chem 2017;36:671-681. Published 2016 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.
authors.affiliation: Upper Midwest Environmental Sciences Center, US Geological Survey, La Crosse, Wisconsin.
authors.forename: Diane L
authors.initials: DL
authors.lastname: Waller
authors.affiliation: Upper Midwest Environmental Sciences Center, US Geological Survey, La Crosse, Wisconsin.
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Bartsch
authors.affiliation: Upper Midwest Environmental Sciences Center, US Geological Survey, La Crosse, Wisconsin.
authors.forename: Kim T
authors.initials: KT
authors.lastname: Fredricks
authors.affiliation: Upper Midwest Environmental Sciences Center, US Geological Survey, La Crosse, Wisconsin.
authors.forename: Lynn A
authors.initials: LA
authors.lastname: Bartsch
authors.affiliation: Upper Midwest Environmental Sciences Center, US Geological Survey, La Crosse, Wisconsin.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Schleis
authors.affiliation: Department of Mathematics, Viterbo University, La Crosse, Wisconsin, USA.
authors.forename: Sheldon H
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000633:toxicity*; D002347:Carps / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D005618:Fresh Water / Q000737:chemistry*; D058865:Introduced Species / Q000639:trends; D049876:Unionidae / Q000187:drug effects* / Q000254:growth & development; D014481:United States; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D002245:Carbon Dioxide
keywords: Benthic macroinvertebrate; Carbon dioxide; Freshwater toxicology; Mollusk toxicology; Unionid