title: Metagenomic insight of nitrogen metabolism in a tannery wastewater treatment plant bioaugmented with the microbial consortium BM-S-1.
abstract: Nitrogen (N) removal in a tannery wastewater treatment plant was significantly enhanced by the bioaugmentation of the novel consortium BM-S-1. In order to identify dominant taxa responsible for N metabolisms in the different stages of the treatment process, Illumina MiSeq Sequencer was used to conduct metagenome sequencing of the microbial communities in the different stages of treatment system, including influent (I), buffering (B), primary aeration (PA), secondary aeration (SA) and sludge digestion (SD). Based on MG-RAST analysis, the dominant phyla were Proteobacteria, Bacteroidetes and Firmicutes in B, PA, SA and SD, whereas Firmicutes was the most dominant in I before augmentation. The augmentation increased the abundance of the denitrification genes found in the genera such as Ralstonia (nirS, norB and nosZ), Pseudomonas (narG, nirS and norB) and Escherichia (narG) in B and PA. In addition, Bacteroides, Geobacter, Porphyromonasand Wolinella carrying nrfA gene encoding dissimilatory nitrate reduction to ammonium were abundantly present in B and PA. This was corroborated with the higher total N removal in these two stages. Thus, metagenomic analysis was able to identify the dominant taxa responsible for dissimilatory N metabolisms in the tannery wastewater treatment system undergoing bioaugmentation. This metagenomic insight into the nitrogen metabolism will contribute to a successful monitoring and operation of the eco-friendly tannery wastewater treatment system.
authors.affiliation: a Department of Systems Biotechnology , Chung-Ang University , Anseong , South Korea.
authors.forename: Woo-Jun
authors.initials: WJ
authors.lastname: Sul
authors.affiliation: b Department of Environmental Engineering , Korea Maritime and Ocean University , Busan , South Korea.
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Kim
authors.affiliation: b Department of Environmental Engineering , Korea Maritime and Ocean University , Busan , South Korea.
authors.forename: Kalu I
authors.initials: KI
authors.lastname: Ekpeghere
authors.affiliation: c Department of Biological Sciences , Viginia Institute of Marine Science , Gloucester Point , Virginia , USA.|d Department of Life Science , Dongguk University-Seoul , Junggu , Seoul , South Korea.
authors.forename: Bongkeun
authors.initials: B
authors.lastname: Song
authors.affiliation: e Department of Life Science , Hallym University , Chuncheon , South Korea.
authors.forename: Bong-Soo
authors.initials: BS
authors.lastname: Kim
authors.affiliation: f BM, Inc. , Kimhae , South Korea.
authors.forename: Hong-Gi
authors.initials: HG
authors.lastname: Kim
authors.affiliation: f BM, Inc. , Kimhae , South Korea.
authors.forename: Jong-Tae
authors.initials: JT
authors.lastname: Kim
authors.affiliation: b Department of Environmental Engineering , Korea Maritime and Ocean University , Busan , South Korea.
authors.forename: Sung-Cheol
authors.initials: SC
authors.lastname: Koh
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D041963:Bacteroidetes / Q000378:metabolism; D004269:DNA, Bacterial / Q000302:isolation & purification; D058440:Denitrification; D000068536:Bacillota / Q000378:metabolism; D005798:Genes, Bacterial; D059013:Microbial Consortia; D058977:Molecular Sequence Annotation; D009584:Nitrogen / Q000302:isolation & purification / Q000378:metabolism*; D020560:Proteobacteria / Q000378:metabolism; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods
chemical_list: D064751:Ammonium Compounds; D004269:DNA, Bacterial; D012722:Sewage; D009584:Nitrogen
keywords: Metagenome analysis; bioaugmentation; denitrification; dissimilatory nitrate reduction to ammonium; nitrogen metabolism; tannery wastewater