title: Zinc supplementation during in vitro maturation increases the production efficiency of cloned pigs.
abstract: Zinc supplementation (0.8 µg/ml) in in vitro maturation (IVM) medium significantly enhances oocyte quality. In this study, we compared the development of somatic cell nuclear transfer (SCNT) embryos produced from conventional IVM (control) and zinc-supplemented IVM oocytes. A total of 1206 and 890 SCNT embryos were produced using control and zinc-supplemented oocytes, respectively, and then were transferred to 11 and 8 recipients, respectively. Five control recipients and three zinc-supplemented recipients became pregnant. Two live piglets and eight mummies were born from two control recipients, and ten live piglets and six stillborn piglets were born from three zinc-supplemented recipients. The production efficiency significantly increased in the zinc-supplemented group (0.33% vs. 3.02%). This report suggests that zinc supplementation in IVM medium improved the production efficiency of cloned pigs.
authors.affiliation: Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Chungbuk, Republic of Korea.
authors.forename: Yubyeol
authors.initials: Y
authors.lastname: Jeon
authors.forename: Junchul David
authors.initials: JD
authors.lastname: Yoon
authors.forename: Lian
authors.initials: L
authors.lastname: Cai
authors.forename: Seon-Ung
authors.initials: SU
authors.lastname: Hwang
authors.forename: Eunhye
authors.initials: E
authors.lastname: Kim
authors.forename: Eunsong
authors.initials: E
authors.lastname: Lee
authors.forename: Eui Bae
authors.initials: EB
authors.lastname: Jeung
authors.forename: Sang-Hwan
authors.initials: SH
authors.lastname: Hyun
authors.forename: Woo Suk
authors.initials: WS
authors.lastname: Hwang
mesh_terms: D000818:Animals; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods / Q000662:veterinary*; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D011256:Pregnancy Outcome; D013552:Swine; D015032:Zinc / Q000008:administration & dosage*
chemical_list: D015032:Zinc