title: Multi-site laser Doppler flowmetry for assessing collateral flow in experimental ischemic stroke: Validation of outcome prediction with acute MRI.
abstract: High variability in infarct size is common in experimental stroke models and affects statistical power and validity of neuroprotection trials. The aim of this study was to explore cerebral collateral flow as a stratification factor for the prediction of ischemic outcome. Transient intraluminal occlusion of the middle cerebral artery was induced for 90 min in 18 Wistar rats. Cerebral collateral flow was assessed intra-procedurally using multi-site laser Doppler flowmetry monitoring in both the lateral middle cerebral artery territory and the borderzone territory between middle cerebral artery and anterior cerebral artery. Multi-modal magnetic resonance imaging was used to assess acute ischemic lesion (diffusion-weighted imaging, DWI), acute perfusion deficit (time-to-peak, TTP), and final ischemic lesion at 24 h. Infarct volumes and typology at 24 h (large hemispheric versus basal ganglia infarcts) were predicted by both intra-ischemic collateral perfusion and acute DWI lesion volume. Collateral flow assessed by multi-site laser Doppler flowmetry correlated with the corresponding acute perfusion deficit using TTP maps. Multi-site laser Doppler flowmetry monitoring was able to predict ischemic outcome and perfusion deficit in good agreement with acute MRI. Our results support the additional value of cerebral collateral flow monitoring for outcome prediction in experimental ischemic stroke, especially when acute MRI facilities are not available.
authors.affiliation: 1 Department of Medicine and Surgery, Laboratory of Experimental Stroke Research, University of Milano-Bicocca, Monza, Italy.|2 PhD Program in Neuroscience, University of Milano-Bicocca, Monza, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Cuccione
authors.affiliation: 1 Department of Medicine and Surgery, Laboratory of Experimental Stroke Research, University of Milano-Bicocca, Monza, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Versace
authors.affiliation: 3 Université de Lyon, CREATIS; CNRS UMR5220; Inserm U1044; INSA-Lyon; Université Lyon 1, Lyon, France.|4 Hospices Civils de Lyon, France.
authors.forename: Tae-Hee
authors.initials: TH
authors.lastname: Cho
authors.affiliation: 1 Department of Medicine and Surgery, Laboratory of Experimental Stroke Research, University of Milano-Bicocca, Monza, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Carone
authors.affiliation: 3 Université de Lyon, CREATIS; CNRS UMR5220; Inserm U1044; INSA-Lyon; Université Lyon 1, Lyon, France.|4 Hospices Civils de Lyon, France.
authors.forename: Lise-Prune
authors.initials: LP
authors.lastname: Berner
authors.affiliation: 3 Université de Lyon, CREATIS; CNRS UMR5220; Inserm U1044; INSA-Lyon; Université Lyon 1, Lyon, France.|4 Hospices Civils de Lyon, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Ong
authors.affiliation: 3 Université de Lyon, CREATIS; CNRS UMR5220; Inserm U1044; INSA-Lyon; Université Lyon 1, Lyon, France.
authors.forename: David
authors.initials: D
authors.lastname: Rousseau
authors.affiliation: 1 Department of Medicine and Surgery, Laboratory of Experimental Stroke Research, University of Milano-Bicocca, Monza, Italy.
authors.forename: Ruiyao
authors.initials: R
authors.lastname: Cai
authors.affiliation: 1 Department of Medicine and Surgery, Laboratory of Experimental Stroke Research, University of Milano-Bicocca, Monza, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Monza
authors.affiliation: 1 Department of Medicine and Surgery, Laboratory of Experimental Stroke Research, University of Milano-Bicocca, Monza, Italy.|5 Milan Center for Neuroscience (NeuroMi), Milan, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Ferrarese
authors.affiliation: 1 Department of Medicine and Surgery, Laboratory of Experimental Stroke Research, University of Milano-Bicocca, Monza, Italy.|5 Milan Center for Neuroscience (NeuroMi), Milan, Italy.
authors.forename: Erik P
authors.initials: EP
authors.lastname: Sganzerla
authors.affiliation: 3 Université de Lyon, CREATIS; CNRS UMR5220; Inserm U1044; INSA-Lyon; Université Lyon 1, Lyon, France.|4 Hospices Civils de Lyon, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Berthezène
authors.affiliation: 3 Université de Lyon, CREATIS; CNRS UMR5220; Inserm U1044; INSA-Lyon; Université Lyon 1, Lyon, France.|4 Hospices Civils de Lyon, France.
authors.forename: Norbert
authors.initials: N
authors.lastname: Nighoghossian
authors.affiliation: 3 Université de Lyon, CREATIS; CNRS UMR5220; Inserm U1044; INSA-Lyon; Université Lyon 1, Lyon, France.
authors.forename: Marlène
authors.initials: M
authors.lastname: Wiart
authors.affiliation: 1 Department of Medicine and Surgery, Laboratory of Experimental Stroke Research, University of Milano-Bicocca, Monza, Italy.|5 Milan Center for Neuroscience (NeuroMi), Milan, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Beretta
authors.affiliation: 6 Université de Lyon, Lyon Neuroscience Research Center, BioRaN team; CNRS UMR5292; Inserm U1028; Université Lyon 1, Lyon, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Chauveau
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000000981:diagnostic imaging* / Q000503:physiopathology; D003097:Collateral Circulation / Q000502:physiology*; D004195:Disease Models, Animal; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D011237:Predictive Value of Tests; D017208:Rats, Wistar; D015203:Reproducibility of Results; D020521:Stroke / Q000000981:diagnostic imaging* / Q000503:physiopathology
keywords: Cerebral collateral flow; Wistar rat; laser Doppler flowmetry; magnetic resonance imaging; middle cerebral artery occlusion