title: Impact of 60-GHz millimeter waves on stress and pain-related protein expression in differentiating neuron-like cells.
abstract: Millimeter waves (MMW) will be increasingly used for future wireless telecommunications. Previous studies on skin keratinocytes showed that MMW could impact the mRNA expression of Transient Receptor Potential cation channel subfamily Vanilloid, member 2 (TRPV2). Here, we investigated the effect of MMW exposure on this marker, as well as on other membrane receptors such as Transient Receptor Potential cation channel subfamily Vanilloid, member 1 (TRPV1) and purinergic receptor P2X, ligand-gated ion channel, 3 (P2 × 3). We exposed the Neuroscreen-1 cell line (a PC12 subclone), in order to evaluate if acute MMW exposures could impact expression of these membrane receptors at the protein level. Proteotoxic stress-related chaperone protein Heat Shock Protein 70 (HSP70) expression level was also assessed. We used an original high-content screening approach, based on fluorescence microscopy, to allow cell-by-cell analysis and to detect any cell sub-population responding to exposure. Immunocytochemistry was done after 24 h MMW exposure of cells at 60.4 GHz, with an incident power density of 10 mW/cm(2) . Our results showed no impact of MMW exposure on protein expressions of HSP70, TRPV1, TRPV2, and P2 × 3. Moreover, no specific cell sub-populations were found to express one of the studied markers at a different level, compared to the rest of the cell populations. However, a slight insignificant increase in HSP70 expression and an increase in protein expression variability within cell population were observed in exposed cells, but controls showed that this was related to thermal effect. Bioelectromagnetics. 37:444-454, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Transcription, Environment Cancer Group, Institute of Research in Environmental and Occupational Health-IRSET, INSERM, University of Rennes 1, Rennes, France.
authors.forename: Alexis J
authors.initials: AJ
authors.lastname: Haas
authors.affiliation: Transcription, Environment Cancer Group, Institute of Research in Environmental and Occupational Health-IRSET, INSERM, University of Rennes 1, Rennes, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Le Page
authors.affiliation: Institute of Electronics and Telecommunications of Rennes-IETR, University of Rennes 1, UMR CNRS, Rennes, France.
authors.forename: Maxim
authors.initials: M
authors.lastname: Zhadobov
authors.affiliation: Institute of Electronics and Telecommunications of Rennes-IETR, University of Rennes 1, UMR CNRS, Rennes, France.
authors.forename: Artem
authors.initials: A
authors.lastname: Boriskin
authors.affiliation: Institute of Electronics and Telecommunications of Rennes-IETR, University of Rennes 1, UMR CNRS, Rennes, France.
authors.forename: Ronan
authors.initials: R
authors.lastname: Sauleau
authors.affiliation: Transcription, Environment Cancer Group, Institute of Research in Environmental and Occupational Health-IRSET, INSERM, University of Rennes 1, Rennes, France. yves.le-drean@univ-rennes1.fr.
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Dréan
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000528:radiation effects*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics*; D009474:Neurons / Q000166:cytology* / Q000528:radiation effects; D016716:PC12 Cells; D011846:Radio Waves / Q000009:adverse effects*; D051381:Rats
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D026901:Membrane Transport Proteins
keywords: 60 GHz; PC12 cells; high-content screening; immunocytochemistry; in vitro