title: (129)Xe NMR Relaxation-Based Macromolecular Sensing.
abstract: We report a (129)Xe NMR relaxation-based sensing approach that exploits changes in the bulk xenon relaxation rate induced by slowed tumbling of a cryptophane-based sensor upon target binding. The amplification afforded by detection of the bulk dissolved xenon allows sensitive detection of targets. The sensor comprises a xenon-binding cryptophane cage, a target interaction element, and a metal chelating agent. Xenon associated with the target-bound cryptophane cage is rapidly relaxed and then detected after exchange with the bulk. Here we show that large macromolecular targets increase the rotational correlation time of xenon, increasing its relaxation rate. Upon binding of a biotin-containing sensor to avidin at 1.5 μM concentration, the free xenon T2 is reduced by a factor of 4.
authors.affiliation: Materials Sciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|‡ Department of Chemistry, University of California , Berkeley, California 94720, United States.
authors.forename: Muller D
authors.initials: MD
authors.lastname: Gomes
authors.affiliation: Materials Sciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|‡ Department of Chemistry, University of California , Berkeley, California 94720, United States.
authors.forename: Phuong
authors.initials: P
authors.lastname: Dao
authors.affiliation: Materials Sciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|‡ Department of Chemistry, University of California , Berkeley, California 94720, United States.
authors.forename: Keunhong
authors.initials: K
authors.lastname: Jeong
authors.affiliation: Materials Sciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|‡ Department of Chemistry, University of California , Berkeley, California 94720, United States.
authors.forename: Clancy C
authors.initials: CC
authors.lastname: Slack
authors.affiliation: ‡ Department of Chemistry, University of California , Berkeley, California 94720, United States.
authors.forename: Christophoros C
authors.initials: CC
authors.lastname: Vassiliou
authors.affiliation: ‡ Department of Chemistry, University of California , Berkeley, California 94720, United States.
authors.forename: Joel A
authors.initials: JA
authors.lastname: Finbloom
authors.affiliation: Materials Sciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|‡ Department of Chemistry, University of California , Berkeley, California 94720, United States.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Francis
authors.affiliation: ‡ Department of Chemistry, University of California , Berkeley, California 94720, United States.|Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: David E
authors.initials: DE
authors.lastname: Wemmer
authors.affiliation: Materials Sciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|‡ Department of Chemistry, University of California , Berkeley, California 94720, United States.
authors.forename: Alexander
authors.initials: A
authors.lastname: Pines
mesh_terms: D015374:Biosensing Techniques; D001710:Biotin / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008670:Metals / Q000737:chemistry; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry; D011083:Polycyclic Compounds; D011485:Protein Binding; D012995:Solubility; D014867:Water / Q000737:chemistry; D014979:Xenon Isotopes / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D046911:Macromolecular Substances; D008670:Metals; D010455:Peptides; D011083:Polycyclic Compounds; D014979:Xenon Isotopes; C523242:cryptophane; D014867:Water; D001710:Biotin