title: Expression Analysis of PIN Genes in Root Tips and Nodules of Medicago truncatula.
abstract: Polar auxin transport is dependent on the family of PIN-formed proteins (PINs), which are membrane transporters of anionic indole-3-acetic acid (IAA(-)). It is assumed that polar auxin transport may be essential in the development and meristematic activity maintenance of Medicago truncatula (M. truncatula) root nodules. However, little is known about the involvement of specific PIN proteins in M. truncatula nodulation. Using real-time quantitative PCR, we analyzed the expression patterns of all previously identified MtPIN genes and compared them between root nodules and root tips of M. truncatula. Our results demonstrated significant differences in the expression level of all 11 genes (MtPIN1-MtPIN11) between examined organs. Interestingly, MtPIN9 was the only PIN gene with higher expression level in root nodules compared to root tips. This result is the first indication of PIN9 transporter potential involvement in M. truncatula nodulation. Moreover, relatively high expression level in root nodules was attributed to MtPINs encoding orthologs of Arabidopsis thaliana PIN5 subclade. PIN proteins from this subclade have been found to localize in the endoplasmic reticulum, which may indicate that the development and meristematic activity maintenance of M. truncatula root nodules is associated with intracellular homeostasis of auxins level and their metabolism in the endoplasmic reticulum.
authors.affiliation: Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. izabela_sanko_sawczenko@sggw.pl.
authors.forename: Izabela
authors.initials: I
authors.lastname: Sańko-Sawczenko
authors.affiliation: Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. barbara_lotocka@sggw.pl.
authors.forename: Barbara
authors.initials: B
authors.lastname: Łotocka
authors.affiliation: Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. weronika_czarnocka@sggw.pl.
authors.forename: Weronika
authors.initials: W
authors.lastname: Czarnocka
mesh_terms: D018506:Gene Expression Regulation, Plant; D046913:Medicago truncatula / Q000235:genetics* / Q000254:growth & development; D018519:Meristem / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D010940:Plant Proteins
keywords: Medicago truncatula; PIN; auxin; root nodule