title: Intrathecal Administration of Flavopiridol Promotes Regeneration in Experimental Model of Spinal Cord Injury.
abstract: OBJECTIVE
  Spinal cord injury (SCI) is a serious condition of the central nervous system and it affects the quality of life and even hampers the day-to-day activity of the patient. In the current study, we investigated the efficacy of intrathecal administration of flavopiridol in an experimental animal model of SCI. The study also aimed at exploring the physiological effects of flavopiridol on neurons, astrocytes and cell cycle regulatory proteins.
  
  
  METHODS
  In vitro scratch wound experiments were performed on female Sprague-Dawley rats (n=23). A complete hemisection to the right of T10 was made, and flavopiridol solution (200 mM, 0.8 nmol flavopiridol/animal) was delivered topically to the lesion site. Cell viability assay, in vitro scratch injury assay, cell cycle analysis using flow cytometry and behavioural assessments were performed.
  
  
  RESULTS
  The local delivery of flavopiridol reduced cavity formation and improved regeneration of neurons with improvement in physiological performance. Flavopiridol also inhibited the migration and proliferation of astrocytes, and at the same time, promoted the survival of neurons.
  
  
  CONCLUSIONS
  Intrathecal administration of flavopiridol can be a promising treatment strategy in patients with SCI and it needs to be validated in patient setting.
authors.affiliation: Second Affiliated Hospital of Nanchang University, Department of Anesthesiology, Nanchang, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Pei-Qian
authors.initials: PQ
authors.lastname: Zhu
authors.forename: Chang-Hua
authors.initials: CH
authors.lastname: Ma
authors.forename: Lin-Hui
authors.initials: LH
authors.lastname: Yuan
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Luo
authors.forename: Guo-Hai
authors.initials: GH
authors.lastname: Xu
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001253:Astrocytes / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D005419:Flavonoids / Q000008:administration & dosage* / Q000494:pharmacology* / Q000627:therapeutic use; D009416:Nerve Regeneration / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects; D010880:Piperidines / Q000008:administration & dosage* / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000473:pathology
chemical_list: D005419:Flavonoids; D010880:Piperidines; C077990:alvocidib