title: Genome-wide analysis of the fructose 1,6-bisphosphate aldolase (FBA) gene family and functional characterization of FBA7 in tomato.
abstract: Fructose 1,6-bisphosphate aldolase (FBA) is a key enzyme in plants that is involved in glycolysis, gluconeogenesis, and the Calvin cycle. FBA genes play significant roles in biotic and abiotic stress responses and also regulate growth and development. Despite the importance of FBA genes, little is known about it in tomato. In this study, we identified 8 FBA genes in tomato and classified them into 2 subgroups based on a phylogenetic tree, gene structures, and conserved motifs. Five (SlFBA1, 2, 3, 4 and 5) and three (SlFBA6, 7, and 8) SlFBA proteins were predicted to be localized in chloroplasts and cytoplasm, respectively. The phylogenetic analysis of FBAs from tomato, Arabidopsis, rice, and other organisms suggested that SlFBA shared the highest protein homology with FBAs from other plants. Synteny analysis indicated that segmental duplication events contributed to the expansion of the tomato FBA family. The expression profiles revealed that all SlFBAs were involved in the response to low and high temperature stresses. SlFBA7 overexpression increased the expression and activities of other main enzymes in Calvin cycle, net photosynthetic rate (Pn), seed size and stem diameter. SlFBA7 overexpression enhanced tolerances in seed germination under suboptimal temperature stresses. Taken together, comprehensive analyses of SlFBAs would provide a basis for understanding of evolution and function of SlFBA family.
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: bingbing-cai@163.com.
authors.forename: Bingbing
authors.initials: B
authors.lastname: Cai
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: lqsdau@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: daymoonxyc@163.com.
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: lyang@sdau.edu.cn.
authors.forename: Long
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: bhg163@163.com.
authors.forename: Huangai
authors.initials: H
authors.lastname: Bi
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: axz@sdau.edu.cn.
authors.forename: Xizhen
authors.initials: X
authors.lastname: Ai
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D005091:Exons; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics* / Q000378:metabolism; D020440:Gene Duplication; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D018525:Germination / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics*; D005810:Multigene Family; D010788:Photosynthesis / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D013696:Temperature
chemical_list: D010940:Plant Proteins; D005634:Fructose-Bisphosphate Aldolase
keywords: Fructose-1,6-bisphosphate aldolase; Genome-wide; Segmental duplication; Suboptimal temperature stress; Tomato