title: Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama).
abstract: This study examines the use of freshwater bream (Abramis brama) as a sentinel organism for genotoxicity assessment of the Danube River using the comet assay. Sampling of bream was performed during February, April, August, and November in 2014 to assess seasonal variation of DNA damage level as a response to genotoxicity in annual cycle. Additionally, concentrations of fecal coliforms and enterococci were analyzed and they indicated a critical to strong level of fecal pollution on investigated locality during annual cycle. Comet assay was performed on blood, liver, and gill cells of bream. DNA damage level was expressed using tail intensity (TI %), Olive tail moment (OTM), and tail length (TL pix). According to TI and OTM, all three tissues had the highest level of DNA damage in August. The lowest level of DNA damage in liver was measured during February, in blood during November, and in gills during April. According to TL, gills had the highest level of DNA damage in February, and liver cells had the lowest level of damage during April. Multiple correspondence analysis (MCA) showed that DNA damage in blood cells is under the strong influence of variations in NO2, NO3-, NH4+levels and also the variation in temperature and oxygen levels. DNA damage in liver cells is highly associated with the variations of Mn, Fe, Cu, Zn, and PO43-levels. DNA damage in gill cells is strongly affected by the variations of As, Cd, Pb, Cr, and COD (Mn) levels. Freshwater bream is shown to be a potentially good indicator organism in genotoxic potential field studies.
authors.affiliation: Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia. jkostic@imsi.rs.|Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000, Belgrade, Serbia. jkostic@imsi.rs.
authors.forename: Jovana
authors.initials: J
authors.lastname: Kostić
authors.affiliation: Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia.
authors.forename: Stoimir
authors.initials: S
authors.lastname: Kolarević
authors.affiliation: Institute for Biological Research "Siniša Stanković", University of Belgrade, Despota Stefana 142, 11000, Belgrade, Serbia.
authors.forename: Margareta
authors.initials: M
authors.lastname: Kračun-Kolarević
authors.affiliation: Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Aborgiba
authors.affiliation: Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000, Belgrade, Serbia.
authors.forename: Zoran
authors.initials: Z
authors.lastname: Gačić
authors.affiliation: Institute for Biological Research "Siniša Stanković", University of Belgrade, Despota Stefana 142, 11000, Belgrade, Serbia.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Lenhardt
authors.affiliation: Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia.
authors.forename: Branka
authors.initials: B
authors.lastname: Vuković-Gačić
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000737:chemistry / Q000187:drug effects; D020552:Comet Assay; D003530:Cyprinidae / Q000097:blood / Q000254:growth & development*; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods*; D005880:Gills / Q000737:chemistry / Q000187:drug effects; D008099:Liver / Q000737:chemistry / Q000187:drug effects; D009153:Mutagens / Q000032:analysis / Q000633:toxicity*; D045483:Rivers / Q000737:chemistry* / Q000382:microbiology; D012621:Seasons; D055771:Serbia; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D009153:Mutagens; D014874:Water Pollutants, Chemical
keywords: Comet assay; Freshwater bream; Genotoxicity; Seasonal variations; Sentinel organism; The Danube River