title: Immunomodulatory effects of Longdan Xiegan Tang on CD4+/CD8+ T cells and associated inflammatory cytokines in rats with experimental autoimmune uveitis.
abstract: Longdan Xiegan Tang (LXT) is a mixture of herbal extracts commonly used in traditional Chinese medicine that may exert immunomodulatory effects for the treatment of autoimmune diseases. However, the detailed mechanisms that mediate the actions of LXT are unclear. The present study induced an experimental autoimmune uveitis (EAU) model in Lewis rats via injection of IRBP1177‑1191 emulsion. The model was used to investigate the effects of LXT on EAU rats and assess the efficacy of LXT by measuring clinical manifestations and histopathological changes caused by EAU. Additionally, alterations in the ratio of CD4+/CD8+‑T cells were determined by flow cytometry, and the expression of interferon (IFN)‑γ, interleukin (IL)‑17, IL‑10 and tumor necrosis factor (TNF)‑α were measured using reverse transcription‑quantitative polymerase chain reaction and enzyme‑linked immunosorbent assay analysis. The results of the present study demonstrate that LXT can efficiently alleviate the symptoms of EAU, inhibit the differentiation of uveitogenic CD4+ T cells and reduce the expression of proinflammatory cytokines, including IFN‑γ, IL‑17 and TNF‑α. Furthermore, LXT promotes the production of IL‑10 and accelerates the recovery of EAU, indicating that the immunomodulatory effects of LXT may potentially be used for the treatment of uveitis.
authors.affiliation: Afﬁliated Eye Hospital, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250002, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Tang
authors.affiliation: Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250002, P.R. China.
authors.forename: Dadong
authors.initials: D
authors.lastname: Guo
authors.affiliation: Department of Ophthalmology, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250002, P.R. China.
authors.forename: Lian
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250002, P.R. China.
authors.forename: Junguo
authors.initials: J
authors.lastname: Guo
authors.affiliation: The First Clinical College, Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, P.R. China.
authors.forename: Fengming
authors.initials: F
authors.lastname: Zheng
authors.affiliation: Afﬁliated Eye Hospital, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250002, P.R. China.
authors.forename: Junkang
authors.initials: J
authors.lastname: Si
authors.affiliation: Afﬁliated Eye Hospital, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250002, P.R. China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Bi
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001327:Autoimmune Diseases / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D056747:Immunomodulation / Q000187:drug effects*; D018836:Inflammation Mediators / Q000378:metabolism*; D008198:Lymph Nodes / Q000276:immunology; D051381:Rats; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000187:drug effects*; D016896:Treatment Outcome; D014605:Uveitis / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D018836:Inflammation Mediators; C550522:longdan xiegan tang