title: Integrating qualitative and quantitative characterization of Prunellae Spica by HPLC-QTOF/MS and HPLC-ELSD.
abstract: The present study was designed to analyze the major constituents in Prunellae Spica and establish a method for simultaneous determination of two constituents contained in Prunellae Spica. High performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-QTOF-MS/MS) technique was used to identify the constituents in the extractive of Prunellae Spica. High performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD) was used to simultaneously quantify two kinds of constituents contained in Prunellae Spica. Principal component analysis (PCA) was applied to compare the similarity and difference among samples from different regions of China. In the present study, 22 compounds were identified and some new fragmental pathways of triterpenic acids were discovered. An accurate and reliable HPLC-ELSD method was developed and validated for the first time to simultaneously quantify multiple constituents, including rosmarinic acid, maslinic acid, corosolic acid, betulin, oleanolic acid, and ursolic acid in the extract of Prunellae Spica. (PCA) revealed some similarities and differences among different samples from different regions of China. In conclusion, our results from this study would be helpful in establishing a scientific and rational quality control method for Prunellae Spica.
authors.affiliation: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 211198, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, China.
authors.forename: Yuan-Jia
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 211198, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: boyangyu59@163.com.
authors.forename: Bo-Yang
authors.initials: BY
authors.lastname: Yu
authors.affiliation: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 211198, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: qijin2006@163.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Qi
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000737:chemistry; D053630:Depsides / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D015394:Molecular Structure; D031347:Prunella / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000737:chemistry; D000097123:Rosmarinic Acid; D009828:Oleanolic Acid / Q000031:analogs & derivatives
chemical_list: D002934:Cinnamates; D053630:Depsides; D004365:Drugs, Chinese Herbal; D014315:Triterpenes; D000097123:Rosmarinic Acid; C113861:corosolic acid; C412811:maslinic acid; D009828:Oleanolic Acid
keywords: HPLC-ELSD; HPLC-QTOF-MS/MS; PCA; Prunella vulgaris; Prunellae Spica