title: Deletion of FtsH11 protease has impact on chloroplast structure and function in Arabidopsis thaliana when grown under continuous light.
abstract: The membrane-integrated metalloprotease FtsH11 of Arabidopsis thaliana is proposed to be dual-targeted to mitochondria and chloroplasts. A bleached phenotype was observed in ftsh11 grown at long days or continuous light, pointing to disturbances in the chloroplast. Within the chloroplast, FtsH11 was found to be located exclusively in the envelope. Two chloroplast-located proteins of unknown function (Tic22-like protein and YGGT-A) showed significantly higher abundance in envelope membranes and intact chloroplasts of ftsh11 and therefore qualify as potential substrates for the FtsH11 protease. No proteomic changes were observed in the mitochondria of 6-week-old ftsh11 compared with wild type, and FtsH11 was not immunodetected in these organelles. The abundance of plastidic proteins, especially of photosynthetic proteins, was altered even during standard growth conditions in total leaves of ftsh11. At continuous light, the amount of photosystem I decreased relative to photosystem II, accompanied by a drastic change of the chloroplast morphology and a drop of non-photochemical quenching. FtsH11 is crucial for chloroplast structure and function during growth in prolonged photoperiod.
authors.affiliation: Department of Chemistry, Umeå University, SE-901 87, Umeå, Sweden.
authors.forename: Raik
authors.initials: R
authors.lastname: Wagner
authors.affiliation: Department of Chemistry, Umeå University, SE-901 87, Umeå, Sweden.
authors.forename: Lotta
authors.initials: L
authors.lastname: von Sydow
authors.affiliation: Department of Chemistry, Umeå University, SE-901 87, Umeå, Sweden.
authors.forename: Harald
authors.initials: H
authors.lastname: Aigner
authors.affiliation: Department of Chemistry, Umeå University, SE-901 87, Umeå, Sweden.|Bioinformatics Infrastructure for Life Sciences (BILS), Linköping, Sweden.
authors.forename: Sergiu
authors.initials: S
authors.lastname: Netotea
authors.affiliation: U1038 INSERM/CEA/UJ, Institut de Recherches en Technologies et Sciences pour le Vivant, Grenoble, Cedex 9, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Brugière
authors.affiliation: Department of Biological and Environmental Sciences, Gothenburg University, 40530, Gothenburg, Sweden.
authors.forename: Lars
authors.initials: L
authors.lastname: Sjögren
authors.affiliation: U1038 INSERM/CEA/UJ, Institut de Recherches en Technologies et Sciences pour le Vivant, Grenoble, Cedex 9, France.
authors.forename: Myriam
authors.initials: M
authors.lastname: Ferro
authors.affiliation: Department of Biological and Environmental Sciences, Gothenburg University, 40530, Gothenburg, Sweden.
authors.forename: Adrian
authors.initials: A
authors.lastname: Clarke
authors.affiliation: Department of Chemistry, Umeå University, SE-901 87, Umeå, Sweden. Christiane.Funk@chem.umu.se.
authors.forename: Christiane
authors.initials: C
authors.lastname: Funk
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D055786:Gene Knockout Techniques; D053263:Gene Regulatory Networks; D045726:Metalloproteases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010641:Phenotype; D017440:Photoperiod
chemical_list: D029681:Arabidopsis Proteins; D002734:Chlorophyll; C506772:FtsH11 protein, Arabidopsis; D045726:Metalloproteases
keywords: envelope; light acclimation