title: Concanavalin A Disrupts the Release of Fibrous Material Necessary for Zygote Formation of a Unicellular Charophycean Alga, Closterium peracerosum-strigosum-littorale Complex.
abstract: The Closterium peracerosum-strigosum-littorale (C. psl.) complex is the best characterized charophycean alga with respect to the processes of sexual reproduction. We examined the effect of concanavalin A (Con A) on physiological and ultrastructural changes during the conjugation of the C. psl. complex. Two heterothallic gametangial cells formed a sexual pair as usual; however, the release of gametes was completely blocked by the addition of Con A. Fluorescein isothiocyanate-labeled Con A bound to the outermost layer of the conjugation papillae of paired cells. In the absence of Con A, the disruption of outer cell walls on the conjugation papillae and the secretion of fibrous materials from the conjugation papillae were observed using a transmission electron microscope, but Con A-treated cells did not show these changes. Instead, a highly electron-dense layer was observed in the outermost papillae, and the excess fibrous materials remained at the inside of the layer. These results suggest that an unknown molecule(s) recognized by Con A is essential for the diffusion of fibrous materials at the conjugation papillae, which is an indispensable step for gamete release during conjugation of the C. psl. complex.
authors.affiliation: Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Tokyo Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Abe
authors.affiliation: Division of Material and Biological Sciences, Graduate School of Science, Japan Women's University, Tokyo Japan.
authors.forename: Sachie
authors.initials: S
authors.lastname: Hori
authors.affiliation: Laboratory of Electron Microscopy, Japan Women's University, Tokyo Japan.
authors.forename: Mamiko
authors.initials: M
authors.lastname: Sato
authors.affiliation: Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, TokyoJapan; Division of Material and Biological Sciences, Graduate School of Science, Japan Women's University, TokyoJapan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sekimoto
keywords: Closterium; Concanavalin A (Con A); charophycean; conjugation; gamete; sexual reproduction