title: Constructing a novel 8-hydroxy-2'-deoxyguanosine electrochemical sensor and application in evaluating the oxidative damages of DNA and guanine.
abstract: 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is commonly identified as a biomarker of oxidative DNA damage. In this work, a novel and facile 8-OHdG sensor was developed based on the multi-walled carbon nanotubes (MWCNTs) modified glassy carbon electrode (GCE). It exhibited good electrochemical responses toward the oxidation of 8-OHdG, and the linear ranges were 5.63×10(-8)-6.08×10(-6)M and 6.08×10(-6)-1.64×10(-5)M, with the detection limit of 1.88×10(-8)M (S/N=3). Moreover, the fabricated sensor was applied for the determination of 8-OHdG generated from damaged DNA and guanine, respectively, and the oxidation currents of 8-OHdG increased along with the damaged DNA and guanine within certain concentrations. These results could be used to evaluate the DNA damage, and provide useful information on diagnosing diseases caused by mutation and deficiency of the immunity system.
authors.affiliation: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070 China.
authors.forename: Zhipan
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070 China. Electronic address: liuxh@nwnu.edu.cn.
authors.forename: Xiuhui
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070 China.
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070 China.
authors.forename: Guofan
authors.initials: G
authors.lastname: Wu
authors.affiliation: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070 China. Electronic address: luxq@nwnu.edu.cn.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Lu
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003217:Conductometry / Q000295:instrumentation*; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004249:DNA Damage / Q000235:genetics*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006147:Guanine / Q000032:analysis / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D037742:Nanotubes, Carbon; D006147:Guanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine
keywords: 8-Hydroxy-2′-deoxyguanosine; Carbon nanotubes; Guanine base; Oxidation assessment; Oxidative DNA damage