title: Eupatilin inhibits the apoptosis in H9c2 cardiomyocytes via the Akt/GSK-3β pathway following hypoxia/reoxygenation injury.
abstract: Eupatilin, a pharmacologically active flavone derived from the Artemisia plant species, has been reported to have anti-oxidant, anti-inflammatory, anti-allergic, and neuroprotective activities against cerebral ischemia/reperfusion (I/R). However, the role of eupatilin in myocardial I/R injury remains unclear. In the present study, we aimed to investigate the potential molecular mechanisms against hypoxia/reoxygenation (H/R) induced cardiomyocytes apoptosis in vitro. Our results showed that eupatilin markedly improved the cell viability and decreased lactate dehydrogenase (LDH) release. Eupatilin also suppressed oxidative stress and apoptosis in H9c2 cells after myocardial I/R injury. Furthermore, eupatilin obviously increased the phosphorylation of Akt and GSK-3β in H9c2 cells. Our results suggested that eupatilin could provide significant cardioprotection against myocardial I/R injury, and the potential mechanisms might involve inhibition of cardiomyocyte apoptosis through activating the Akt/GSK-3β signaling pathway.
authors.affiliation: Department of Cardiology, Affiliated Tenth People's Hospital of TongJi University, Shanghai 200072, China.
authors.forename: Zengyong
authors.initials: Z
authors.lastname: Qiao
authors.affiliation: Department of Cardiology, Affiliated Tenth People's Hospital of TongJi University, Shanghai 200072, China. Electronic address: xuyaweicardio@163.com.
authors.forename: Ya-Wei
authors.initials: YW
authors.lastname: Xu
authors.affiliation: Basic Medical College of Shanghai Jiaotong University, Shanghai 200025, China. Electronic address: jingyuphd@126.com.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002316:Cardiotonic Agents / Q000494:pharmacology; D002460:Cell Line; D005419:Flavonoids / Q000494:pharmacology*; D000071679:Glycogen Synthase Kinase 3 beta / Q000378:metabolism*; D000860:Hypoxia / Q000473:pathology*; D015428:Myocardial Reperfusion Injury; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects; D010100:Oxygen / Q000633:toxicity*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D002316:Cardiotonic Agents; D005419:Flavonoids; C045325:eupatilin; D000071679:Glycogen Synthase Kinase 3 beta; D051057:Proto-Oncogene Proteins c-akt; D010100:Oxygen
keywords: Apoptosis; Eupatilin; Myocardial ischemia/reperfusion (I/R); Oxidative stress