title: Genetic association between SNPs in the DGAT1 gene and milk production traits in Murrah buffaloes.
abstract: This study identified polymorphisms in the DGAT1 gene in Murrah buffaloes and investigated the associations to milk production and quality traits (milk, fat and protein yields and percentages, somatic cell count). Genomic DNA was extracted from hair follicles collected from the tail of 196 females. Three SNPs were identified in DGAT1 gene by sequencing. Statistical analyses were performed to verify the linkage and the association between polymorphisms and traits. The estimated value of r (2) between two SNPs in exon 17 (g.11,783G > A and g.11,785 T > C) was 0.029. SNP g.11,785 T > C was significantly associated (P < 0.05) to fat and protein percentage. Dominance effect was significant for milk and fat yields and protein percentage (P < 0.05). The additive effect of the SNP g.11,785 T > C was significant for protein production and somatic cell count (P < 0.05). It indicates that assisted marker selection might be done with considerations to balance production and udder health.
authors.affiliation: Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil. anafreitas.unesp07@gmail.com.
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: de Freitas
authors.affiliation: Universidade Tecnológica Federal do Paraná (UTFPR), Estrada para Boa Esperança, km 04, 85660-000, Dois Vizinhos, PR, Brazil.
authors.forename: Gregório Miguel Ferreira
authors.initials: GM
authors.lastname: de Camargo
authors.affiliation: Departamento de Ciências Exatas, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil.
authors.forename: Nedenia Bonvino
authors.initials: NB
authors.lastname: Stafuzza
authors.affiliation: Faculdade de Ciências Agrárias (FCA), Universidade Federal da Grande Dourados (UFGD), Rodovia Dourados a Itahum km 12, 79804-970, Dourados, MS, Brazil.
authors.forename: Rusbel Raul
authors.initials: RR
authors.lastname: Aspilcueta-Borquis
authors.affiliation: Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil.
authors.forename: Guilherme Costa
authors.initials: GC
authors.lastname: Venturini
authors.affiliation: Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil.
authors.forename: Marina Mortati
authors.initials: MM
authors.lastname: Dias
authors.affiliation: Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil.
authors.forename: Diercles Francisco
authors.initials: DF
authors.lastname: Cardoso
authors.affiliation: Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil.
authors.forename: Humberto
authors.initials: H
authors.lastname: Tonhati
mesh_terms: D000818:Animals; D001938:Brazil; D002020:Buffaloes / Q000235:genetics* / Q000502:physiology; D003612:Dairying; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics*; D005260:Female; D005838:Genotype; D007774:Lactation / Q000235:genetics* / Q000502:physiology; D008892:Milk / Q000378:metabolism*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D014329:Tropical Climate
chemical_list: D051048:Diacylglycerol O-Acyltransferase
keywords: Bubalus bubalis; Diacylglycerol O-acyltransferase1; Fat percentage; Polymorphism; Protein percentage