title: Enhanced xylose fermentation and hydrolysate inhibitor tolerance of Scheffersomyces shehatae for efficient ethanol production from non-detoxified lignocellulosic hydrolysate.
abstract: Effective conversion of xylose into ethanol is important for lignocellulosic ethanol production. In the present study, UV-C mutagenesis was used to improve the efficiency of xylose fermentation. The mutated Scheffersomyces shehatae strain TTC79 fermented glucose as efficiently and xylose more efficiently, producing a higher ethanol concentration than the wild-type. A maximum ethanol concentration of 29.04 g/L was produced from 71.31 g/L xylose, which was 58.95 % higher than that of the wild-type. This mutant also displayed significantly improved hydrolysate inhibitors tolerance and increased ethanol production from non-detoxified lignocellulosic hydrolysates. The ethanol yield, productivity and theoretical yield by TTC79 from sugarcane bagasse hydrolysate were 0.46 g/g, 0.20 g/L/h and 90.61 %, respectively, while the corresponding values for the wild-type were 0.20 g/g, 0.04 g/L/h and 39.20 %, respectively. These results demonstrate that S. shehatae TTC79 is a useful non-recombinant strain, combining efficient xylose consumption and high inhibitor tolerance, with potential for application in ethanol production from lignocellulose hydrolysates.
authors.affiliation: Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140 Thailand.
authors.forename: Srisuda
authors.initials: S
authors.lastname: Senatham
authors.affiliation: Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140 Thailand.
authors.forename: Thada
authors.initials: T
authors.lastname: Chamduang
authors.affiliation: Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140 Thailand.
authors.forename: Yotin
authors.initials: Y
authors.lastname: Kaewchingduang
authors.affiliation: Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140 Thailand ; Microbial Biotechnology Unit, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140 Thailand.
authors.forename: Anon
authors.initials: A
authors.lastname: Thammasittirong
authors.affiliation: Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140 Thailand ; Microbial Biotechnology Unit, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140 Thailand.
authors.forename: Malee
authors.initials: M
authors.lastname: Srisodsuk
authors.affiliation: Biorefinery Center, Institute of Food Research, Norwich, NR4 7UA UK.
authors.forename: Adam
authors.initials: A
authors.lastname: Elliston
authors.affiliation: National Collection of Yeast Cultures, Institute of Food Research, Norwich, NR4 7UA UK.
authors.forename: Ian N
authors.initials: IN
authors.lastname: Roberts
authors.affiliation: Biorefinery Center, Institute of Food Research, Norwich, NR4 7UA UK.
authors.forename: Keith W
authors.initials: KW
authors.lastname: Waldron
authors.affiliation: Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140 Thailand ; Microbial Biotechnology Unit, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140 Thailand.
authors.forename: Sutticha Na-Ranong
authors.initials: SN
authors.lastname: Thammasittirong
keywords: Ethanol; Hydrolysate inhibitor; Lignocellulose; Scheffersomyces shehatae; UV-mutagenesis