title: Xyloglucans from flaxseed kernel cell wall: Structural and conformational characterisation.
abstract: The structure of ethanol precipitated fraction from 1M KOH extracted flaxseed kernel polysaccharides (KPI-EPF) was studied for better understanding the molecular structures of flaxseed kernel cell wall polysaccharides. Based on methylation/GC-MS, NMR spectroscopy, and MALDI-TOF-MS analysis, the dominate sugar residues of KPI-EPF fraction comprised of (1,4,6)-linked-β-d-glucopyranose (24.1mol%), terminal α-d-xylopyranose (16.2mol%), (1,2)-α-d-linked-xylopyranose (10.7mol%), (1,4)-β-d-linked-glucopyranose (10.7mol%), and terminal β-d-galactopyranose (8.5mol%). KPI-EPF was proposed as xyloglucans: The substitution rate of the backbone is 69.3%; R1 could be T-α-d-Xylp-(1→, or none; R2 could be T-α-d-Xylp-(1→, T-β-d-Galp-(1→2)-α-d-Xylp-(1→, or T-α-l-Araf-(1→2)-α-d-Xylp-(1→; R3 could be T-α-d-Xylp-(1→, T-β-d-Galp-(1→2)-α-d-Xylp-(1→, T-α-l-Fucp-(1→2)-β-d-Galp-(1→2)-α-d-Xylp-(1→, or none. The Mw of KPI-EPF was calculated to be 1506kDa by static light scattering (SLS). The structure-sensitive parameter (ρ) of KPI-EPF was calculated as 1.44, which confirmed the highly branched structure of extracted xyloglucans. This new findings on flaxseed kernel xyloglucans will be helpful for understanding its fermentation properties and potential applications.
authors.affiliation: Department of Food Science, University of Guelph, 50 Stone Road E., Guelph N1 G 2W1, Canada; Guelph Research and Development Centre, Agriculture and Agri-Food Canada, 93 Stone Road W., Guelph N1 G 5C9, Canada. Electronic address: dingh@uoguelph.ca.
authors.forename: Huihuang H
authors.initials: HH
authors.lastname: Ding
authors.affiliation: Department of Food Science, University of Guelph, 50 Stone Road E., Guelph N1 G 2W1, Canada; Guelph Research and Development Centre, Agriculture and Agri-Food Canada, 93 Stone Road W., Guelph N1 G 5C9, Canada. Electronic address: Steve.Cui@agr.gc.ca.
authors.forename: Steve W
authors.initials: SW
authors.lastname: Cui
authors.affiliation: Department of Food Science, University of Guelph, 50 Stone Road E., Guelph N1 G 2W1, Canada. Electronic address: dgoff@uoguelph.ca.
authors.forename: H Douglas
authors.initials: HD
authors.lastname: Goff
authors.affiliation: SKLF, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. Electronic address: chenjie@jiangnan.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Guelph Research and Development Centre, Agriculture and Agri-Food Canada, 93 Stone Road W., Guelph N1 G 5C9, Canada. Electronic address: qingbin@uoguelph.ca.
authors.forename: Qingbin
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Guelph Research and Development Centre, Agriculture and Agri-Food Canada, 93 Stone Road W., Guelph N1 G 5C9, Canada. Electronic address: Qi.Wang@agr.gc.ca.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
mesh_terms: D002473:Cell Wall / Q000737:chemistry*; D019597:Flax; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D012639:Seeds / Q000737:chemistry*; D014990:Xylans / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D005936:Glucans; D014990:Xylans; C029353:xyloglucan
keywords: Conformation; Dietary fibre; Flaxseed; Structure; Xyloglucan