title: The influence of light quality on the accumulation of flavonoids in tobacco (Nicotiana tabacum L.) leaves.
abstract: Flavonoids are important secondary metabolites in plants regulated by the environment. To analyze the effect of light quality on the accumulation of flavonoids, we performed a rapid analysis of flavonoids in extracts of tobacco leaves using UHPLC-QTOF. A total of 12 flavonoids were detected and identified in tobacco leaves, which were classified into flavonoid methyl derivatives and flavonoid glycoside derivatives according to the groups linked to the flavonoid core. Correlation analysis was further conducted to investigate the effect of different wavelengths of light on their accumulation. The content of flavonoid methyl derivatives was positively correlated with the proportions of far-red light (FR; 716-810nm) and near-infrared light (NIR; 810-2200nm) in the sunlight spectrum and negatively correlated with the proportion of ultraviolet (UV-A; 350-400nm) and the red/far-red ratio (R/FR). By contrast, the content of flavonoid glycoside derivatives was positively correlated with the proportion of UV-A and the R/FR, and negatively correlated with FR and NIR. The results indicated that light quality with higher proportions of FR and NIR increases the activity of flavonoid methyltransferases but suppresses the activity of flavonoid glycoside transferases. While a high proportion of UV-A and a high R/FR can increase flavonoid glycoside transferase activity but suppress flavonoid methyltransferase activity.
authors.affiliation: College of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan Province 450002, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Fu
authors.affiliation: College of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan Province 450002, PR China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ji
authors.affiliation: College of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan Province 450002, PR China. Electronic address: zhaomingqin@126.com.
authors.forename: Mingqin
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan Province 450002, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: He
authors.affiliation: College of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan Province 450002, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan Province 450002, PR China.
authors.forename: Yiding
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan Province 450002, PR China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liu
authors.affiliation: College of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan Province 450002, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Niu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism; D007259:Infrared Rays; D008027:Light; D013058:Mass Spectrometry; D008780:Methyltransferases / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D014026:Nicotiana / Q000737:chemistry* / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D005419:Flavonoids; D008780:Methyltransferases; D005964:Glucosyltransferases
keywords: Flavonoid glycoside derivatives; Flavonoid methyl derivatives; Flavonoids; Light quality; UHPLC-QTOF