title: Nanometric emulsions encapsulating solid particles as alternative carriers for intracellular delivery.
abstract: Formulate nanometric oil droplets for encapsulating solid nanoparticles and assess their interactions with cells.
  
  
  
  Soybean oil droplets, stabilized by Pluronic F68 surfactant, incorporating hydrophobically modified fluorescent silica, nanoparticles were obtained. Cytotoxicity over time, internalization, subsequent intracellular localization and internalization pathways were assessed by microscopy (fluoresence and TEM) in vitro with HeLa cells.
  
  
  
  Oil droplets encapsulating solid nanoparticles are readily internalized by HeLa cells like free nanoparticles but the intracellular localization differs (nanoemulsions less colocalized with lysosomes) as well as internalization pathway is used (nanoemulsions partially internalized by nonendocytic transport). No cytotoxicity could be observed for either particles tested.
  
  
  
  Our results confirm that nanometric emulsions encapsulating solid nanoparticles can be used for alternative and multifunctional intracellular delivery.
authors.affiliation: École Normale Supérieure - PSL Research University, Département de Chimie, 24 rue Lhomond, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ. Paris 06, PASTEUR, F-75005 Paris, France.|CNRS, UMR 8640 PASTEUR, F-75005 Paris, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Quignard
authors.affiliation: Institut de Biologie Paris-Seine FR3631, Service de Microscopie Electronique, Université Pierre et Marie Curie, 9 Quai Saint Bernard, 75005 Paris, France.
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Frébourg
authors.affiliation: École Normale Supérieure - PSL Research University, Département de Chimie, 24 rue Lhomond, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ. Paris 06, PASTEUR, F-75005 Paris, France.|CNRS, UMR 8640 PASTEUR, F-75005 Paris, France.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: École Normale Supérieure - PSL Research University, Département de Chimie, 24 rue Lhomond, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ. Paris 06, PASTEUR, F-75005 Paris, France.|CNRS, UMR 8640 PASTEUR, F-75005 Paris, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Fattaccioli
mesh_terms: D004337:Drug Carriers / Q000737:chemistry*; D004655:Emulsions / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D020442:Poloxamer / Q000737:chemistry; D012822:Silicon Dioxide / Q000008:administration & dosage* / Q000493:pharmacokinetics; D013024:Soybean Oil / Q000737:chemistry* / Q000493:pharmacokinetics
chemical_list: D004337:Drug Carriers; D004655:Emulsions; D005456:Fluorescent Dyes; D020442:Poloxamer; D012822:Silicon Dioxide; D013024:Soybean Oil
keywords: hybrid nanoparticles; intracellular delivery; passive internalization