title: A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast.
abstract: Commercial fructo-oligosaccharides (FOS) are predominantly produced from sucrose by transfructosylation process that presents a maximum theoretical yield below 0.60gFOSgSucrose(-1). To obtain high-content FOS, costly purification is generally employed. Additionally, high-content FOS can be produced from inulin by using endo-inulinases. However, commercial endo-inulinases have not been extensively used in scale-up production of FOS. In the present study, a one-step bioprocess that integrated endo-inulinase production, FOS fermentation, and non-FOS sugars removal into one reactor was proposed to produce high-content FOS from inulin. The bioprocess was implemented by a recombinant yeast strain JZHΔS-TSC, in which a heterologous endo-inulinase gene was expressed and the inherent invertase gene SUC2 was disrupted. FOS fermentation at 40°C from 200g/L chicory inulin presented the maximun titer, yield, and productivity of 180.2±0.8g/L, 0.9gFOSgInulin(-1), and 7.51±0.03g/L/h, respectively. This study demonstrated that the one-step bioprocess was simple and highly efficient.
authors.affiliation: Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; University of Chinese Academy of Sciences, Beijing 100039, China. Electronic address: wangda@qibebt.ac.cn.
authors.forename: Da
authors.initials: D
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China. Electronic address: lifl@qibebt.ac.cn.
authors.forename: Fu-Li
authors.initials: FL
authors.lastname: Li
authors.affiliation: Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China. Electronic address: wangsa@qibebt.ac.cn.
authors.forename: Shi-An
authors.initials: SA
authors.lastname: Wang
mesh_terms: D005632:Fructose / Q000737:chemistry*; D005818:Genetic Engineering; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D007444:Inulin / Q000378:metabolism*; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D005632:Fructose; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase
keywords: Fructo-oligosaccharides; Inulin; One-step bioprocess; Yeast