title: Seroprevalence of tuberculosis in domesticated elk (Cervus canadensis) in Korea.
abstract: Tuberculosis is a contagious disease in animals, primarily cattle, although it also affects wild animals and humans. There are few data on the state of tuberculosis in domesticated elk (Cervus canadensis) in Korea. In order to investigate tuberculosis in elk, the effectiveness of an enzyme linked immunosorbent assay (ELISA) using MPB70 and MPB83 antigens was compared with the tuberculin skin test (TST), and seroprevalence was measured with this assay using serum samples collected from domesticated elk herds in Korea. The respective sensitivities of the MPB70 and MPB83 ELISAs were 51.9% (95% CI 42.0-61.6) and 49.1% (95% CI 39.3-58.9), and their specificities were 100.0% (95% CI 92.6-100.0) and 97.9% (95% CI 88.9-100.0), respectively, in comparison with the TST. The herd prevalence ranged from 50 to 80% and the mean herd seropositive rate was 67.7% (21 of 31). Of 819 serum samples, 163 (19.9%) were seropositive, and the within-region prevalence ranged from 18.5-58.0%. In conclusion, the ELISA using the MPB70 and MPB83 antigens showed moderate sensitivity and high specificity compared to TST in elk, and tuberculosis was assumed to be fairly prevalent in domesticated elk in Korea.
authors.affiliation: Chungbuk Veterinary Service Laboratory, 380-230 Chungju, Republic of Korea.
authors.forename: Shin-Seok
authors.initials: SS
authors.lastname: Kang
authors.affiliation: Chungbuk Veterinary Service Laboratory, 380-230 Chungju, Republic of Korea; Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chungbuk National University, 362-763 Cheungju, Republic of Korea.
authors.forename: Hyeon-Seop
authors.initials: HS
authors.lastname: Byeon
authors.affiliation: Bacterial Disease, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, 430-757 Anyang, Republic of Korea.
authors.forename: Bok Kyung
authors.initials: BK
authors.lastname: Ku
authors.affiliation: Animal Genetics Resources Station, National Institute of Animal Science, Rural Development Administration, 590-832 Namwon, Republic of Korea.
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, 220-710 Wonju, Republic of Korea.
authors.forename: Jiro
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, 220-710 Wonju, Republic of Korea.
authors.forename: Jeongim
authors.initials: J
authors.lastname: Woo
authors.affiliation: Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chungbuk National University, 362-763 Cheungju, Republic of Korea.
authors.forename: Byeongwoo
authors.initials: B
authors.lastname: Ahn
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, 220-710 Wonju, Republic of Korea.
authors.forename: Soojin
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, 220-710 Wonju, Republic of Korea.
authors.forename: Sezim
authors.initials: S
authors.lastname: Monoldorova
authors.affiliation: Gangwondo Veterinary Service Laboratory, 220-170 Wonju, Republic of Korea.
authors.forename: Chan-Ho
authors.initials: CH
authors.lastname: Park
authors.affiliation: Department of Microbiology, College of Medicine, Yonsei University, 120-752 Seoul, Republic of Korea.
authors.forename: Sang-Nae
authors.initials: SN
authors.lastname: Cho
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, 220-710 Wonju, Republic of Korea. Electronic address: bojeon@yonsei.ac.kr.
authors.forename: Bo-Young
authors.initials: BY
authors.lastname: Jeon
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D003670:Deer / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D056910:Republic of Korea / Q000453:epidemiology; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014374:Tuberculin Test; D014376:Tuberculosis / Q000453:epidemiology / Q000662:veterinary*
chemical_list: D000942:Antigens, Bacterial
keywords: ELISA; Elk; MPB70; MPB83; Seroprevalence; Tuberculosis