title: Detection and Analysis of Ebola Virus in Sierra Leone-China Friendship Biosafety Laboratory from March 11 to April 20, 2015.
abstract: Ebola virus disease reemerged in Western Africa in 2014. Chinese Center for Disease Control and Prevention dispatched the first Ebola virus (EBOV) detection team to run newly established Sierra Leone-China Friendship Biological Safety Laboratory. The aims of study were to understand epidemiology, clinical manifestations and survival time of EBOV in patient's blood. A total of 913 specimens were tested between March 11 and April 20, 2015. EBOV positivity occurred in 7.37% of the blood and 0.53% in throat swabs. Most commonly reported symptoms of laboratory confirmed patients were intense fatigue, anorexia, and fever. EBOV RNAs persisted in blood for almost 4 weeks and the real-time RT-PCR Ct values showed close correlation with the sampling time after onset.
authors.affiliation: Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China.
authors.forename: Huan Yu
authors.initials: HY
authors.lastname: Wang
authors.affiliation: National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China.
authors.forename: Hai Jun
authors.initials: HJ
authors.lastname: DU
authors.affiliation: National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Nie
authors.affiliation: National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China.
authors.forename: Jing Dong
authors.initials: JD
authors.lastname: Song
authors.affiliation: National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China; Collaborative Innovation Center Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310003, Zhejiang, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Xiao
authors.affiliation: National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China.
authors.forename: Wen Wen
authors.initials: WW
authors.lastname: Lei
authors.affiliation: National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China.
authors.forename: Jian Qiang
authors.initials: JQ
authors.lastname: Guo
authors.affiliation: National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China.
authors.forename: He Jiang
authors.initials: HJ
authors.lastname: Wei
authors.affiliation: National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Cai
authors.affiliation: National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China.
authors.forename: Yan Hai
authors.initials: YH
authors.lastname: Wang
authors.affiliation: National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone.
authors.forename: Gerald
authors.initials: G
authors.lastname: Bangura
authors.affiliation: Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone.
authors.forename: Idrissa Laybohr
authors.initials: IL
authors.lastname: Kamara
authors.affiliation: National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Sierra Leone-China Friendship Biological Safety Laboratory, Freetown 999127, Sierra-Leone; State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China; Collaborative Innovation Center Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310003, Zhejiang, China; Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiao Ping
authors.initials: XP
authors.lastname: Dong
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001769:Blood / Q000821:virology; D002648:Child; D002675:Child, Preschool; D029043:Ebolavirus / Q000302:isolation & purification*; D019142:Hemorrhagic Fever, Ebola / Q000097:blood* / Q000453:epidemiology / Q000821:virology; D006801:Humans; D007223:Infant; D007753:Laboratories; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D012807:Sierra Leone / Q000453:epidemiology; D055815:Young Adult