title: Synthesis and characterization of a novel near-infrared fluorescent probe for applications in imaging A549 cells.
abstract: OBJECTIVE
  To design and synthesize a novel near-infrared (NIR) fluorescent probe based on indocyanine Green (ICG), that can be applied in imaging living cells.
  
  
  RESULTS
  A highly fluorescent novel NIR fluorescent probe (IR-793) was synthesized in two steps. IR-793 had better fluorescence and optical stability than ICG. In addition, no obvious cytotoxicity effect of IR-793 was observed and cell viability was above 75% at the maximum concentration (120 nM). IR-793 also exhibited good performance in imaging living A549 cells.
  
  
  CONCLUSIONS
  IR-793, a novel NIR fluorescent probe that is stable, low-cost, highly fluorescent and low cytotoxicity, has been designed and synthesized for imaging living cells.
authors.affiliation: School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, China.|School of Biological Science and Medical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Suzhou, 215123, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xing
authors.affiliation: School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, China.|School of Biological Science and Medical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Suzhou, 215123, China.
authors.forename: Gaoxin
authors.initials: G
authors.lastname: Zhou
authors.affiliation: College of Life Sciences, Binzhou University, Binzhou, 256600, China.
authors.forename: Chunlong
authors.initials: C
authors.lastname: Sun
authors.affiliation: Chia-tai Tianqing Pharmaceutical Group Co. Ltd., No.699-8 Xuanwu Blvd., Nanjing, 210042, China.
authors.forename: Huanqing
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, China.|School of Biological Science and Medical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Suzhou, 215123, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.affiliation: School of Chemistry & Chemical Engineering, Southeast University, Nanjing, 210096, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zong
authors.affiliation: School of Chemistry & Chemical Engineering, Southeast University, Nanjing, 210096, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Cai
authors.affiliation: School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, China. seujimin@126.com.|School of Biological Science and Medical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Suzhou, 215123, China. seujimin@126.com.
authors.forename: Min
authors.initials: M
authors.lastname: Ji
mesh_terms: D000072283:A549 Cells; D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007208:Indocyanine Green / Q000737:chemistry*; D015394:Molecular Structure; D061848:Optical Imaging / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D007208:Indocyanine Green
keywords: A549 cells; Fluorescence imaging; Fluorescent dye; IR-793; Imaging of living cells; Indocyanine Green; Living cells