title: Mineralization of pyrene induced by interaction between Ochrobactrum sp. PW and ryegrass in spiked soil.
abstract: This study was conducted to investigate the capability of pyrene-degrading bacterium Ochrobactrum sp. PW and ryegrass (Lolium multiflorum) grown alone and in combination on the degradation of pyrene in soil. After 60 days of ryegrass growth, plant biomass, pyrene-degrading microbial mass, soil enzyme activity (catalase activity and polyphenol oxidase activity) and residual concentration of pyrene in soils were determined. Higher dissipation rates were observed in PW inoculation treatments: ryegrass+PW rhizosphere soil (RP-r) and ryegrass+PW non-rhizosphere soil (RP-nr), than planting of ryegrass alone, rhizosphere (R-r) or non-rhizosphere (R-nr). The inoculation with PW significantly (p<0.05) increased the dry weight of ryegrass root and shoot, nearly 2.8 and 3.3 times higher than ryegrass treatment. The pyrene-degrading microbial mass indicated that a much larger mass of bacteria, actinobacteria were present in RP treatment. The catalase activity in all different treatments were significantly (p<0.05) higher than in with treatment R-nr, and the polyphenol oxidase activity was also significantly (p<0.05) increased by inoculation with PW, leading to enhanced mineralization of pyrene from soil. Our results suggest that adding of PAHs-degrading bacteria to soil can enhance remediation of PAHs contaminated soil, while improving plant growth.
authors.affiliation: Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Tuo
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Lianshuang
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Soil Environmental Sciences, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Qiao
authors.affiliation: Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Dexun
authors.initials: D
authors.lastname: Zou
authors.affiliation: Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China; Beijing City Environmental Pollution Control and Resource Reuse Engineering Research Center, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: environbiol@mail.buct.edu.cn.
authors.forename: Aijun
authors.initials: A
authors.lastname: Lin
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D008129:Lolium / Q000378:metabolism*; D042041:Ochrobactrum / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes / Q000737:chemistry / Q000378:metabolism*; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D012987:Soil; D012989:Soil Pollutants
keywords: Combined remediation; Degradation bacteria; PW; Pyrene; Ryegrass