title: MicroRNA 433 regulates nonsense-mediated mRNA decay by targeting SMG5 mRNA.
abstract: Nonsense-mediated mRNA decay (NMD) is a RNA quality surveillance system for eukaryotes. It prevents cells from generating deleterious truncated proteins by degrading abnormal mRNAs that harbor premature termination codon (PTC). However, little is known about the molecular regulation mechanism underlying the inhibition of NMD by microRNAs.
  
  
  
  The present study demonstrated that miR-433 was involved in NMD pathway via negatively regulating SMG5. We provided evidence that (1) overexpression of miR-433 significantly suppressed the expression of SMG5 (P < 0.05); (2) Both mRNA and protein expression levels of TBL2 and GADD45B, substrates of NMD, were increased when SMG5 was suppressed by siRNA; (3) Expression of SMG5, TBL2 and GADD45B were significantly increased by miR-433 inhibitor (P < 0.05). These results together illustrated that miR-433 regulated NMD by targeting SMG5 mRNA.
  
  
  
  Our study highlights that miR-433 represses nonsense mediated mRNA decay. The miR-433 targets 3'-UTR of SMG5 and represses the expression of SMG5, whereas NMD activity is decreased when SMG5 is decreased. This discovery provides evidence for microRNA/NMD regulatory mechanism.
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Zhenfa
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China. renzq@mail.hzau.edu.cn.|The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China. renzq@mail.hzau.edu.cn.
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Ren
mesh_terms: D020413:3' Untranslated Regions; D000943:Antigens, Differentiation / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D015536:Down-Regulation; D019204:GTP-Binding Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D059365:Nonsense Mediated mRNA Decay; D012333:RNA, Messenger / Q000235:genetics*; D015854:Up-Regulation; D000097642:GADD45 Proteins
chemical_list: D020413:3' Untranslated Regions; D000943:Antigens, Differentiation; D002352:Carrier Proteins; D019204:GTP-Binding Proteins; D035683:MicroRNAs; D012333:RNA, Messenger; C086470:GADD45B protein, human; D000097642:GADD45 Proteins; C548883:MIRN433 microRNA, human; C515801:SMG5 protein, human; C402405:TBL2 protein, human
keywords: NMD; SMG5; miR-433