title: Role of the suppressor of cytokine signaling 2 (SOCS2) during meningoencephalitis caused by Bovine herpesvirus 5 (BoHV-5).
abstract: The role of suppressors of cytokine signaling (SOCS) in meningoencephalitis caused by Bovine herpesvirus 5 (BoHV-5) was evaluated by intracranial infection in C57BL/6 wild-type mice (WT) and SOCS2 deficient mice (SOCS2(-/-)). Both infected groups presented weight loss, ruffled fur and hunched posture. Additionally, infected SOCS2(-/-) mice showed swollen chamfer and progressive depression. Infected WT animals developed mild meningitis, characterized by infiltration of mononuclear cells. Moreover, viral DNA was detected in liver and lung from infected WT group. This group also showed elevated brain levels of IFN-γ, IL-10, CXCL1 and CCL5, when compared with non-infected WT animals. Brain inflammation was exacerbated in infected SOCS2(-/-) mice with widespread distribution of the virus and increased brain levels of TNF-α, IFN-γ, IL-10, IL-12, CXCL1 and CCL5, when compared with WT infected mice. Moreover, infected SOCS2 deficient mice exhibited reduced brain mRNA expression of IFNα and IFNβ and increased expression of mRNA of SOCS1, compared with infected WT mice. Taken together, our study provides an insight into the role of SOCS2 in modulating the immune response to BoHV-5 infection.
authors.affiliation: Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: alinesb.bio@gmail.com.
authors.forename: Aline
authors.initials: A
authors.lastname: Aparecida Silva Barbosa
authors.affiliation: Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: alicefv_vet@yahoo.com.br.
authors.forename: Alice
authors.initials: A
authors.lastname: Freitas Versiani
authors.affiliation: Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: laris.fonseca@gmail.com.
authors.forename: Larissa
authors.initials: L
authors.lastname: Fonseca da Cunha Sousa
authors.affiliation: Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: mirandas.aline@gmail.com.
authors.forename: Aline
authors.initials: A
authors.lastname: Silva de Miranda
authors.affiliation: Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: marcela.gasparini@pirbright.ac.uk.
authors.forename: Marcela Ribeiro
authors.initials: MR
authors.lastname: Gasparini
authors.affiliation: Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: fatimacbrant@gmail.com.
authors.forename: Fátima
authors.initials: F
authors.lastname: Brant
authors.affiliation: Departamentos de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: danivets@gmail.com.
authors.forename: Daniele Gonçalves
authors.initials: DG
authors.lastname: Silva
authors.affiliation: Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: iracemaluisa@yahoo.com.br.
authors.forename: Iracema
authors.initials: I
authors.lastname: Luisa Quintino-de-Carvalho
authors.affiliation: Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: fredsori@gmail.com.
authors.forename: Frederico
authors.initials: F
authors.lastname: Marianetti Soriani
authors.affiliation: Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: fdafonseca@cpqrr.fiocruz.br.
authors.forename: Flávio
authors.initials: F
authors.lastname: Guimarães da Fonseca
authors.affiliation: Departamentos de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: anilton@icb.ufmg.br.
authors.forename: Anilton
authors.initials: A
authors.lastname: César Vasconcelos
authors.affiliation: Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: aloicul@yahoo.com.
authors.forename: Lucíola
authors.initials: L
authors.lastname: da Silva Barcelos
authors.affiliation: Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: mmtex.ufmg@gmail.com.
authors.forename: Mauro
authors.initials: M
authors.lastname: Martins Teixeira
authors.affiliation: Translational Psychiatry Program, Department of Psychiatry and Behavioral Sciences, School of Medicine, University of Texas Health Science Center at Houston, TX, USA. Electronic address: altexr@gmail.com.
authors.forename: Antônio
authors.initials: A
authors.lastname: Lúcio Teixeira
authors.affiliation: Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: machadofs@gmail.com.
authors.forename: Fabiana Simão
authors.initials: FS
authors.lastname: Machado
authors.affiliation: Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: edelfb@icb.ufmg.br.
authors.forename: Edel Figueiredo
authors.initials: EF
authors.lastname: Barbosa-Stancioli
authors.affiliation: Departamentos de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: milenerachid@gmail.com.
authors.forename: Milene Alvarenga
authors.initials: MA
authors.lastname: Rachid
mesh_terms: D000818:Animals; D001921:Brain / Q000276:immunology / Q000503:physiopathology / Q000821:virology*; D002417:Cattle; D018946:Chemokine CCL5 / Q000235:genetics; D054360:Chemokine CXCL1 / Q000235:genetics; D016207:Cytokines / Q000235:genetics; D004279:DNA, Viral; D006566:Herpesviridae Infections / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D025881:Herpesvirus 5, Bovine / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D016898:Interferon-alpha / Q000235:genetics; D016899:Interferon-beta / Q000235:genetics; D008099:Liver / Q000821:virology; D008168:Lung / Q000821:virology; D008590:Meningoencephalitis / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D050826:Suppressor of Cytokine Signaling Proteins / Q000172:deficiency / Q000235:genetics* / Q000276:immunology / Q000502:physiology*
chemical_list: C503460:Ccl5 protein, mouse; D018946:Chemokine CCL5; D054360:Chemokine CXCL1; C475895:Cxcl1 protein, mouse; D016207:Cytokines; D004279:DNA, Viral; C554329:Ifna1 protein, mouse; D016898:Interferon-alpha; C491409:Socs2 protein, mouse; D050826:Suppressor of Cytokine Signaling Proteins; D016899:Interferon-beta
keywords: Bovine herpesvirus 5; Brain; Inflammation; Mice; SOCS2