title: CHARACTERIZATION OF VIRULENCE GENES AND ANTIMICROBIAL RESISTANCE OF LUNG PATHOGENIC ESCHERICHIA COLI ISOLATES IN FOREST MUSK DEER (MOSCHUS BEREZOVSKII).
abstract: This study investigated genotypic diversity, 26 virulence genes, and antimicrobial susceptibility of lung pathogenic Escherichia coli (LPEC) isolated from forest musk deer. Associations between virulence factors (VFs) and phylogenetic group, between antimicrobial resistance (AMR) and phylogenetic group, and between AMR and VFs were subsequently assessed. The results showed 30 LPEC isolated were grouped into seven different clusters (A, B, C, D, E, F, and G). The detection rates of crl (90%), kpsMT II (76.67%), mat (76.67%), and ompA (80%) were over 75%. The most frequent types of resistance were to amoxicillin (100%), sulfafurazole (100%), ampicillin (96.67%), and tetracycline (96.67%), with 93.33% (n = 28) of isolates resistant to more than eight types of drugs. There were significant relationships between resistance to cefalotin and the presence of iucD(a) (P < 0.001), papC (P = 0.032), and kpsMT II (P = 0.028); between resistance to chloromycetin and the presence of irp2 (P = 0.004) and vat (P = 0.047); between resistance to nalidixic acid and the presence of crl (P = 0.002) and iucD(a) (P = 0.004); and between resistance to ampicillin/sulbactam and the presence of vat (P = 0.013). These results indicated there could be some association between resistance and VFs, and there is a great need for the prudent use of antimicrobial agents in LPEC.
authors.forename: Xi
authors.initials: X
authors.lastname: Luo
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Cheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.forename: Lei
authors.initials: L
authors.lastname: Dai
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Li-kou
authors.initials: LK
authors.lastname: Zou
authors.forename: Bei
authors.initials: B
authors.lastname: Li
authors.forename: Jiu-Jin
authors.initials: JJ
authors.lastname: Xiao
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003670:Deer; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D014644:Genetic Variation; D005838:Genotype; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000662:veterinary*; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antimicrobial resistance; forest musk deer; genotype; lung pathogenic Escherichia coli; virulence factors