title: Umbilical cord-derived mesenchymal stem cells inhibit growth and promote apoptosis of HepG2 cells.
abstract: Hepatocellular carcinoma is the fifth most common type of cancer worldwide and remains difficult to treat. The aim of this study was to investigate the effects of mesenchymal stem cells (MSCs) derived from the umbilical cord (UC‑MSCs) on HepG2 hepatocellular carcinoma cells. UC‑MSCs were co‑cultured with HepG2 cells and biomarkers of UC‑MSCs were analyzed by flow cytometry. mRNA and protein expression of genes were determined by reverse transcription‑polymerase chain reaction and flow cytometry, respectively. Passage three and seven UC‑MSCs expressed CD29, CD44, CD90 and CD105, whereas CD34 and CD45 were absent on these cells. Co‑culture with UC‑MSCs inhibited proliferation and promoted apoptosis of HepG2 cells in a time‑dependent manner. The initial seeding density of UC‑MSCs also influenced the proliferation and apoptosis of HepG2 cells, with an increased number of UC‑MSCs causing enhanced proliferation inhibition and cell apoptosis. Co‑culture with UC‑MSCs downregulated mRNA and protein expression of α‑fetoprotein (AFP), Bcl‑2 and Survivin in HepG2 cells. Thus, UC‑MSCs may inhibit growth and promote apoptosis of HepG2 cells through downregulation of AFP, Bcl‑2 and Survivin. US-MSCs may be used as a novel therapy for treating hepatocellular carcinoma in the future.
authors.affiliation: Department of Gastroenterology, The 2nd Affiliated Hospital of Kunming Medical University, Yunnan Research Center for Liver Diseases, Kunming, Yunnan 650033, P.R. China.
authors.forename: Ying-Mei
authors.initials: YM
authors.lastname: Tang
authors.affiliation: Department of General Surgery, Yunnan Provincial 1st People's Hospital, Kunming, Yunnan 650032, P.R. China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Bao
authors.affiliation: Department of Gastroenterology, The 2nd Affiliated Hospital of Kunming Medical University, Yunnan Research Center for Liver Diseases, Kunming, Yunnan 650033, P.R. China.
authors.forename: Jin-Hui
authors.initials: JH
authors.lastname: Yang
authors.affiliation: Department of Ophthamology, The 2nd Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650033, P.R. China.
authors.forename: Lin-Kun
authors.initials: LK
authors.lastname: Ma
authors.affiliation: Department of Gastroenterology, The 2nd Affiliated Hospital of Kunming Medical University, Yunnan Research Center for Liver Diseases, Kunming, Yunnan 650033, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, The 2nd Affiliated Hospital of Kunming Medical University, Yunnan Research Center for Liver Diseases, Kunming, Yunnan 650033, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, The 2nd Affiliated Hospital of Kunming Medical University, Yunnan Research Center for Liver Diseases, Kunming, Yunnan 650033, P.R. China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, The 2nd Affiliated Hospital of Kunming Medical University, Yunnan Research Center for Liver Diseases, Kunming, Yunnan 650033, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Sha
authors.affiliation: Department of Gastroenterology, The 2nd Affiliated Hospital of Kunming Medical University, Yunnan Research Center for Liver Diseases, Kunming, Yunnan 650033, P.R. China.
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, The 2nd Affiliated Hospital of Kunming Medical University, Yunnan Research Center for Liver Diseases, Kunming, Yunnan 650033, P.R. China.
authors.forename: Hua-Mei
authors.initials: HM
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, The 2nd Affiliated Hospital of Kunming Medical University, Yunnan Research Center for Liver Diseases, Kunming, Yunnan 650033, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, The 2nd Affiliated Hospital of Kunming Medical University, Yunnan Research Center for Liver Diseases, Kunming, Yunnan 650033, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, The 2nd Affiliated Hospital of Kunming Medical University, Yunnan Research Center for Liver Diseases, Kunming, Yunnan 650033, P.R. China.
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Li
mesh_terms: D000954:Antigens, Surface / Q000378:metabolism; D017209:Apoptosis; D015415:Biomarkers; D014408:Biomarkers, Tumor; D002454:Cell Differentiation; D049109:Cell Proliferation; D018920:Coculture Techniques; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D014408:Biomarkers, Tumor; D012333:RNA, Messenger