title: Acute Exercise Improves Insulin Clearance and Increases the Expression of Insulin-Degrading Enzyme in the Liver and Skeletal Muscle of Swiss Mice.
abstract: The effects of exercise on insulin clearance and IDE expression are not yet fully elucidated. Here, we have explored the effect of acute exercise on insulin clearance and IDE expression in lean mice. Male Swiss mice were subjected to a single bout of exercise on a speed/angle controlled treadmill for 3-h at approximately 60-70% of maximum oxygen consumption. As expected, acute exercise reduced glycemia and insulinemia, and increased insulin tolerance. The activity of AMPK-ACC, but not of IR-Akt, pathway was increased in the liver and skeletal muscle of trained mice. In an apparent contrast to the reduced insulinemia, glucose-stimulated insulin secretion was increased in isolated islets of these mice. However, insulin clearance was increased after acute exercise and was accompanied by increased expression of the insulin-degrading enzyme (IDE), in the liver and skeletal muscle. Finally, C2C12, but not HEPG2 cells, incubated at different concentrations of 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) for 3-h, showed increased expression of IDE. In conclusion, acute exercise increases insulin clearance, probably due to an augmentation of IDE expression in the liver and skeletal muscle. The elevated IDE expression, in the skeletal muscle, seems to be mediated by activation of AMPK-ACC pathway, in response to exercise. We believe that the increase in the IDE expression, comprise a safety measure to maintain glycemia at or close to physiological levels, turning physical exercise more effective and safe.
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Mirian A
authors.initials: MA
authors.lastname: Kurauti
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.|Laboratory of Exercise Physiology, Department of Physical Therapy, University of Pernambuco (UPE), Pernambuco, PE, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Freitas-Dias
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Ferreira
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Jean F
authors.initials: JF
authors.lastname: Vettorazzi
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Tarlliza R
authors.initials: TR
authors.lastname: Nardelli
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Hygor N
authors.initials: HN
authors.lastname: Araujo
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.|Department of Physiological Sciences, Center of Biological Sciences, Federal University of Santa Catarina (UFSC), Florianopolis, SC, Brazil.
authors.forename: Gustavo J
authors.initials: GJ
authors.lastname: Santos
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Everardo M
authors.initials: EM
authors.lastname: Carneiro
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Antonio C
authors.initials: AC
authors.lastname: Boschero
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.|Laboratory of Health Sciences, Department of Physiopathology, State University of Montes Claros (UNIMONTES), Montes Claros, MG, Brazil.
authors.forename: Luiz F
authors.initials: LF
authors.lastname: Rezende
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: José M
authors.initials: JM
authors.lastname: Costa-Júnior
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D004789:Enzyme Activation; D056945:Hep G2 Cells; D006801:Humans; D006868:Hydrolysis; D007328:Insulin / Q000378:metabolism*; D007339:Insulysin; D007515:Islets of Langerhans / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000201:enzymology*; D010101:Oxygen Consumption; D010805:Physical Conditioning, Animal
chemical_list: D007328:Insulin; D055372:AMP-Activated Protein Kinases; D007339:Insulysin