title: Muscle specific nucleus ambiguus neurons isolation and culturing.
abstract: Peripheral nerve injury leads to a regenerative state. However, the reinnervation process is highly non-selective. Growing axons are often misrouted and establish aberrant synapsis to abductor or adductor muscles. Determining the complex properties of abductor and adductor motoneurons in a neuron culture, may lay the groundwork for future studies on axon guidance, leading to a clinical treatment for a selective reinnervation.
  
  
  
  In the present study we develop a neuron culture protocol to isolate recurrent laryngeal nerve abductor and adductor motoneurons in order to study their unique properties. Comparison with existing methods the best period to perform the present protocol for postnatal rat cranial motoneurons isolation was determined. In addition, the method allows identification of specific motoneurons from other primary motoneurons and interneurons within brainstem.
  
  
  
  The present protocol will allow investigators to perform targeted and novel studies of the mechanisms of peripheral nerve regeneration.
authors.affiliation: Department of Cell Biology and Anatomy, New York Medical College, Basic Sciences Building, 15 Dana Road, Valhalla, New York 10595, United States; Department of Otolaryngology-Head and Neck Surgery, Columbia University, Medical Center, 630 West, 168th Street, New York, NY 10032, United States. Electronic address: I_HernandezMorato@nymc.edu.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Hernandez-Morato
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Columbia University, Medical Center, 630 West, 168th Street, New York, NY 10032, United States; Department of Otolaryngology, New York Eye and Ear Infirmary of Mount Sinai, 310E, 14th Street, 6th Floor, New York Eye and Ear Infirmary, New York, NY 10003, United States.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Pitman
authors.affiliation: Department of Cell Biology and Anatomy, New York Medical College, Basic Sciences Building, 15 Dana Road, Valhalla, New York 10595, United States.
authors.forename: Sansar
authors.initials: S
authors.lastname: Sharma
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002232:Carbocyanines / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004525:Efferent Pathways / Q000502:physiology; D005154:Facial Nerve / Q000502:physiology; D005453:Fluorescence; D008526:Medulla Oblongata / Q000166:cytology*; D009046:Motor Neurons / Q000502:physiology*; D009132:Muscles / Q000294:innervation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D002232:Carbocyanines; C024286:3,3'-dioctadecylindocarbocyanine
keywords: Motoneurons; Neuron culture; Neuron isolation; Recurrent laryngeal nerve; Reinnervation