title: Dysregulation of Autophagy, Mitophagy, and Apoptotic Genes in the Medial Temporal Lobe Cortex in an Ischemic Model of Alzheimer's Disease.
abstract: Ischemic brain damage is a pathological incident that is often linked with medial temporal lobe cortex injury and finally its atrophy. Post-ischemic brain injury associates with poor prognosis since neurons of selectively vulnerable ischemic brain areas are disappearing by apoptotic program of neuronal death. Autophagy has been considered, after brain ischemia, as a guardian against neurodegeneration. Consequently, we have examined changes in autophagy (BECN 1), mitophagy (BNIP 3), and apoptotic (caspase 3) genes in the medial temporal lobe cortex with the use of quantitative reverse-transcriptase PCR following transient 10-min global brain ischemia in rats with survival 2, 7, and 30 days. The intense significant overexpression of BECN 1 gene was noted on the 2nd day, while on days 7-30 the expression of this gene was still upregulated. BNIP 3 gene was downregulated on the 2nd day, but on days 7-30 post-ischemia, there was a significant reverse tendency. Caspase 3 gene, associated with apoptotic neuronal death, was induced in the same way as BNIP 3 gene after brain ischemia. Thus, the demonstrated changes indicate that the considerable dysregulation of expression of BECN 1, BNIP 3, and caspase 3 genes may be connected with a response of neuronal cells in medial temporal lobe cortex to transient complete brain ischemia.
authors.affiliation: First Department of Neurology, Institute of Psychiatry and Neurology, Warsaw, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Ułamek-Kozioł
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Kocki
authors.affiliation: Department of Biology and Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Bogucka-Kocka
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Petniak
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Gil-Kulik
authors.affiliation: Laboratory of Ischemic and Neurodegenerative Brain Research, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Januszewski
authors.affiliation: Warsaw Higher Humanistic School, Warsaw, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Bogucki
authors.affiliation: Department of Rehabilitation and Orthopaedics, Medical University of Lublin, Lublin, Poland.
authors.forename: Mirosław
authors.initials: M
authors.lastname: Jabłoński
authors.affiliation: Department of Neonate and Infant Pathology, Medical University of Lublin, Lublin, Poland.
authors.forename: Wanda
authors.initials: W
authors.lastname: Furmaga-Jabłońska
authors.affiliation: Department of Clinical Psychology, Medical University of Lublin, Lublin, Poland.
authors.forename: Judyta
authors.initials: J
authors.lastname: Brzozowska
authors.affiliation: Department of Pathophysiology, Medical University of Lublin, Lublin, Poland.
authors.forename: Stanisław J
authors.initials: SJ
authors.lastname: Czuczwar
authors.affiliation: Laboratory of Ischemic and Neurodegenerative Brain Research, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Pluta
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D001343:Autophagy / Q000235:genetics / Q000502:physiology; D000071186:Beclin-1 / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D006323:Heart Arrest; D008565:Membrane Proteins / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D063306:Mitophagy / Q000235:genetics / Q000502:physiology; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D013702:Temporal Lobe / Q000378:metabolism*; D013997:Time Factors
chemical_list: C505650:BNIP3 protein, rat; D000071186:Beclin-1; C512829:Becn1 protein, rat; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; C505410:Casp3 protein, rat; D053148:Caspase 3
keywords: Alzheimer’s disease; BECN 1; BNIP 3; brain ischemia; caspase 3; genes; rat; selective vulnerability; temporal cortex