title: Grafting of mesenchymal stem cell-seeded small intestinal submucosa to repair the deep partial-thickness burns.
abstract: Regenerative medicine provides many treatments for burn wounds, of which cell-seeded substitutes are encouraging for large and deep burns. To assess the feasibility of mesenchymal stem cell (MSC)-seeded small intestinal submucosa (SIS) to repair the deep partial-thickness burns, a rat study was performed.
  
  
  
  The burn model was created by contacting the dorsal surface directly with boiled water for 10 seconds. MSCs at passage 3 were seeded on the SIS before implantation. Three days after burn injury, the grafts were implanted onto the burn area. At 3, 7, 14 and 21 days post implantation, gross observation and histological assessments were performed.
  
  
  
  SIS alone and MSC-seeded SIS were able to accelerate the burn wound closure by enhancing granulation tissue formation, increasing wound maturity, improving revascularization, and inducing the proliferation of neo-epidermal cells. Additionally, MSC-seeded SIS was much more effective than SIS alone for the repair of deep partial-thickness burns.
  
  
  
  Both SIS and MSC-seeded SIS were able to repair the large and deep burn wounds and the loaded MSCs possessed positive effects to accelerate the wound closure in a rat model.
authors.affiliation: a Department of Burns and Plastic Surgery , The Affiliated Hospital of Southwest Medical University , Luzhou , China.
authors.forename: Xing
authors.initials: X
authors.lastname: Guo
authors.affiliation: b Department of Bone and Joint Surgery , The Affiliated Hospital of Southwest Medical University , Luzhou , China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xia
authors.affiliation: b Department of Bone and Joint Surgery , The Affiliated Hospital of Southwest Medical University , Luzhou , China.
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Lu
authors.affiliation: b Department of Bone and Joint Surgery , The Affiliated Hospital of Southwest Medical University , Luzhou , China.
authors.forename: Zhong-Jie
authors.initials: ZJ
authors.lastname: Zhang
authors.affiliation: b Department of Bone and Joint Surgery , The Affiliated Hospital of Southwest Medical University , Luzhou , China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
authors.affiliation: c Department of Oncology , The Affiliated Hospital of Southwest Medical University , Luzhou , China.
authors.forename: Wei-Ling
authors.initials: WL
authors.lastname: Li
authors.affiliation: a Department of Burns and Plastic Surgery , The Affiliated Hospital of Southwest Medical University , Luzhou , China.
authors.forename: Ai-Bing
authors.initials: AB
authors.lastname: Xiong
authors.affiliation: d Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy , West China Hospital, Sichuan University , Chengdu , China.
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.affiliation: b Department of Bone and Joint Surgery , The Affiliated Hospital of Southwest Medical University , Luzhou , China.
authors.forename: Mei-Yun
authors.initials: MY
authors.lastname: Tan
authors.affiliation: e Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine , The University of Hong Kong , Hong Kong SAR , China.|f Shenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies , Orthopaedics Research Center, Peking University Shenzhen Hospital , Shenzhen , China.
authors.forename: Yong-Can
authors.initials: YC
authors.lastname: Huang
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology* / Q000628:therapy*; D049109:Cell Proliferation; D004817:Epidermis / Q000473:pathology; D006097:Granulation Tissue / Q000473:pathology; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000473:pathology*; D007421:Intestine, Small / Q000473:pathology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D014945:Wound Healing; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D014841:von Willebrand Factor
keywords: burns; mesenchymal stem cells; small intestinal submucosa; wound closure