title: Tanshinone IIA ameliorates apoptosis of cardiomyocytes induced by endoplasmic reticulum stress.
abstract: The fat-soluble diterpenoids tanshinone IIA (TSA) is the major active element of Danshen, which has widespread cardioprotective effect. However, the mechanism of its beneficial effect on cardiomyocytes has not been fully investigated. Here, we aim to demonstrate that TSA ameliorates apoptosis of cardiomyocytes activated by endoplasmic reticulum stress (ERS). Primary cultures of neonatal rat cardiomyocytes are used, in which ERS-mediated apoptosis is induced by tunicamycin (Tm). Apoptosis of cardiomyocytes are detected by Hoechst staining and caspase 3 activity analysis. Protein expression of ERS markers are detected by Western blot, and level of miroRNA-133 (miR-133) is detected by real-time polymerase chain reaction. Tm treatment significantly triggers the apoptosis and ERS of cardiomyocytes. TSA dramatically ameliorates apoptosis and ERS of cardiomyocytes induced by Tm. Interestingly, level of miR-133 is reduced by Tm treatment, which is reversed by TSA. The cardioprotective effect of TSA on apoptosis and ERS of cardiomyocytes is blocked by anti-miR-133. These results suggest that TSA protects cardiomyocytes through ameliorated ERS-mediated apoptosis, which may be resulted from upregulation of miR-133.
authors.affiliation: Department of Emergency Medicine, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Emergency Medicine, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Emergency Medicine, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Huawen
authors.initials: H
authors.lastname: Chen
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D015153:Blotting, Western; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000502:physiology; D035683:MicroRNAs / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological / Q000187:drug effects / Q000502:physiology; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D045784:Abietanes; D002316:Cardiotonic Agents; C549446:MIRN133 microRNA, rat; D035683:MicroRNAs; C021751:tanshinone; D014415:Tunicamycin
keywords: Tanshinone IIA; apoptosis; cardiomyocytes; endoplasmic reticulum stress