title: The potential role of Piezo2 in the mediation of visceral sensation.
abstract: Piezo2 is an important mechano-gated ion channel that is involved in light touch sensitivity and inflammatory allodynia. However, current research has focused on the function of Piezo2 in somatic sensation but not in visceral sensation. The present study aimed to investigate the role of Piezo2 in visceral sensation of mechanically innocuous and noxious stimuli under physiological and hyperalgesic conditions using rats as a model organism. Neonatal enema with acetic acid induced visceral hypersensitivity. Intrathecal administration of Piezo2-short hairpin RNA (shRNA) reduced Piezo2 expression in lumbosacral dorsal root ganglia (DRG) at both the mRNA and protein levels. Piezo2 knock-down in DRG attenuated visceral sensation to innocuous stimuli in control rats and to both innocuous and noxious stimuli in rats with neonatal irritation. Compared with control rats, Piezo2 was not up-regulated in irritated rats at the mRNA or protein levels in thoracolumbar or lumbosacral DRGs, while TRPV1 was up-regulated in lumbosacral DRGs. These data suggest a potential role of Piezo2 in the mediation of visceral sensation.
authors.affiliation: Department of Gastroenterology, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China.
authors.forename: Jianna
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Lei
authors.affiliation: Department of Gastroenterology, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China. Electronic address: xcqys@126.com.
authors.forename: Changqing
authors.initials: C
authors.lastname: Xu
mesh_terms: D019342:Acetic Acid / Q000008:administration & dosage; D000818:Animals; D000831:Animals, Newborn; D003106:Colon / Q000187:drug effects / Q000502:physiology; D005727:Ganglia, Spinal / Q000502:physiology*; D055785:Gene Knockdown Techniques; D007473:Ion Channels / Q000235:genetics / Q000502:physiology*; D008161:Lumbosacral Region; D008297:Male; D059225:Nociception / Q000502:physiology; D010812:Physical Stimulation; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D014110:Touch / Q000502:physiology*; D014781:Viscera / Q000502:physiology*
chemical_list: D007473:Ion Channels; D012333:RNA, Messenger; C000589204:piezo2 protein, rat; D019342:Acetic Acid
keywords: Piezo2; TRPV1; Visceral sensation; shRNA