title: Vitellogenin and vitellogenin receptor gene expression and 20-hydroxyecdysone concentration in Macrobrachium rosenbergii exposed to chlordecone.
abstract: Chlordecone is a persistent organochlorine pesticide widely used in Guadeloupe (French West Indies) to control the banana weevil Cosmopolites sordidus. Although it was previously highlighted that chlordecone may affect the reproduction and growth of vertebrate species, little information is available on the chlordecone effects in invertebrates. The present study investigated the effects of chlordecone on a hormone and a protein having key roles in reproduction and growth of the decapod crustacean Macrobrachium rosenbergii, by measuring the 20-hydroxyecdysone concentration, vitellogenin, and vitellogenin receptor gene expression, as well as the bioconcentration of chlordecone in exposed prawns. First, the results revealed that chlordecone was accumulated in M. rosenbergii. Then, it was found that Vg and VgR gene expression were increased in male and female M. rosenbergii exposed to chlordecone for 90 and 240 days, while the 20-hydroxyecdysone concentrations were decreased. This work suggests that chlordecone accumulates in prawn tissues and could affect key molecules involved in the reproduction and the growth of the invertebrate M. rosenbergii. However, many questions remain unresolved regarding the impacts of chlordecone on growth and reproduction and the signaling pathways responsible for these effects, as well as the potential role of confounding factors present in in situ studies.
authors.affiliation: Laboratory of Animal Ecology and Ecotoxicology (LEAE), Centre of Analytical Research and Technology (CART), University of Liège, 15 Allée du Six Aout, 4000, Liège, Belgium. anne.lafontaine@ulg.ac.be.
authors.forename: Anne
authors.initials: A
authors.lastname: Lafontaine
authors.affiliation: Center for Protein Engineering, Functional Genomics and Plant Molecular Imaging, University of Liège, 27 Boulevard du Rectorat, 4000, Liège, Belgium.|PhytoSYSTEMS, University of Liège, 27 Boulevard du Rectorat, 4000, Liège, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Hanikenne
authors.affiliation: Normandie University, ULH, UMR I-02 SEBIO, FR CNRS 3730 SCALE, 25 rue Philippe Lebon, 76058, Le Havre, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Boulangé-Lecomte
authors.affiliation: Normandie University, ULH, UMR I-02 SEBIO, FR CNRS 3730 SCALE, 25 rue Philippe Lebon, 76058, Le Havre, France.
authors.forename: Joëlle
authors.initials: J
authors.lastname: Forget-Leray
authors.affiliation: Laboratory of Animal Ecology and Ecotoxicology (LEAE), Centre of Analytical Research and Technology (CART), University of Liège, 15 Allée du Six Aout, 4000, Liège, Belgium.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Thomé
authors.affiliation: Laboratory of Animal Ecology and Ecotoxicology (LEAE), Centre of Analytical Research and Technology (CART), University of Liège, 15 Allée du Six Aout, 4000, Liège, Belgium.
authors.forename: Eric
authors.initials: E
authors.lastname: Gismondi
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000378:metabolism*; D007631:Chlordecone / Q000633:toxicity*; D004441:Ecdysterone / Q000378:metabolism*; D004527:Egg Proteins / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000633:toxicity; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D033541:Palaemonidae / Q000187:drug effects* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014819:Vitellogenins / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D060829:Arthropod Proteins; D004527:Egg Proteins; D052244:Endocrine Disruptors; D007306:Insecticides; D011956:Receptors, Cell Surface; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; C030474:vitellogenin receptor; D004441:Ecdysterone; D007631:Chlordecone
keywords: 20-Hydroxyecdysone; Chlordecone; Macrobrachium rosenbergii; Vitellogenin; Vitellogenin receptor