title: Inhibitory Effects of Silver Nanoparticles on Removal of Organic Pollutants and Sulfate in an Anaerobic Biological Wastewater Treatment Process.
abstract: The increasing use of silver nanoparticles (AgNPs) in commercial products and industrial processes raises issues regarding the toxicity of sludge biomass in biological wastewater treatment plants, due to potential antimicrobial properties. This study investigated the effects of AgNPs on removal of organic pollutants and sulfate in an anaerobic biological sulfate reduction process. At AgNPs concentrations of up to 10 mg/L, no significant inhibition of sulfate and COD removal was observed. However, at higher concentrations (50-200 mg/L) sulfate and COD removal efficiencies were significantly decreased to 51.8% and 33.6%, respectively. Sulfate and COD reduction followed first-order kinetics at AgNPs concentrations of up to 10 mg/L and second-order kinetics at AgNPs concentrations of 50-200 mg/L. Lactate dehydrogenase release profiles showed increases in cytotoxicity at AgNPs concentrations greater than 50 mg/L suggesting cell membrane disruption. Analysis of extracellular polymeric substances (EPS) from sulfidogenic sludge biomass and of Fourier transform infrared (FT-IR) spectra showed a decrease in concentrations of carbohydrates, proteins, humic substances, and lipids in the presence of AgNPs. Moreover, the interaction of AgNPs with sludge biomass and the damage caused to cell walls were confirmed through scanning electron microscopy with energy dispersive X-ray spectroscopy.
authors.forename: Kashif
authors.initials: K
authors.lastname: Rasool
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Lee
mesh_terms: D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000378:metabolism*; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D004305:Dose-Response Relationship, Drug; D053768:Metal Nanoparticles / Q000633:toxicity*; D009930:Organic Chemicals / Q000302:isolation & purification / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D012834:Silver / Q000633:toxicity*; D013431:Sulfates / Q000302:isolation & purification / Q000378:metabolism*; D062065:Wastewater / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism; D018508:Water Purification / Q000379:methods*
chemical_list: D009930:Organic Chemicals; D010100:Oxygen; D012834:Silver; D013431:Sulfates; D062065:Wastewater; D014874:Water Pollutants, Chemical