title: Determination of dimethyl trisulfide in rabbit blood using stir bar sorptive extraction gas chromatography-mass spectrometry.
abstract: Cyanide poisoning by accidental or intentional exposure poses a severe health risk. The current Food and Drug Administration approved antidotes for cyanide poisoning can be effective, but each suffers from specific major limitations concerning large effective dosage, delayed onset of action, or dependence on enzymes generally confined to specific organs. Dimethyl trisulfide (DMTS), a sulfur donor that detoxifies cyanide by converting it into thiocyanate (a relatively nontoxic cyanide metabolite), is a promising next generation cyanide antidote. Although a validated analytical method to analyze DMTS from any matrix is not currently available, one will be vital for the approval of DMTS as a therapeutic agent against cyanide poisoning. Hence, a stir bar sorptive extraction (SBSE) gas chromatography - mass spectrometry (GC-MS) method was developed and validated for the analysis of DMTS from rabbit whole blood. Following acid denaturation of blood, DMTS was extracted into a polydimethylsiloxane-coated stir bar. The DMTS was then thermally desorbed from the stir bar and analyzed by GC-MS. The limit of detection of DMTS using this method was 0.06μM with dynamic range from 0.5-100μM. For quality control standards, the precision, as measured by percent relative standard deviation, was below 10%, and the accuracy was within 15% of the nominal concentration. The method described here will allow further investigations of DMTS as a promising antidote for cyanide poisoning.
authors.affiliation: Department of Chemistry and Biochemistry, South Dakota State University, Avera Health and Science, Box 2202, Brookings, SD, 57007, United States.
authors.forename: Erica
authors.initials: E
authors.lastname: Manandhar
authors.affiliation: Department of Chemistry and Biochemistry, South Dakota State University, Avera Health and Science, Box 2202, Brookings, SD, 57007, United States.
authors.forename: Nujud
authors.initials: N
authors.lastname: Maslamani
authors.affiliation: Department of Chemistry, Sam Houston State University, P.O. Box 2117, Huntsville, TX, 77341, United States.
authors.forename: Ilona
authors.initials: I
authors.lastname: Petrikovics
authors.affiliation: Analytical Toxicology Division, US Army Medical Research Institute of Chemical Defense, 2900 Rickets Point Road, Aberdeen Proving Ground, MD, 21010, United States.
authors.forename: Gary A
authors.initials: GA
authors.lastname: Rockwood
authors.affiliation: Department of Chemistry and Biochemistry, South Dakota State University, Avera Health and Science, Box 2202, Brookings, SD, 57007, United States. Electronic address: brian.logue@sdstate.edu.
authors.forename: Brian A
authors.initials: BA
authors.lastname: Logue
mesh_terms: D000818:Animals; D000931:Antidotes / Q000302:isolation & purification*; D004129:Dimethylpolysiloxanes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D011817:Rabbits; D013440:Sulfides / Q000097:blood / Q000302:isolation & purification*; D014481:United States
chemical_list: D000931:Antidotes; D004129:Dimethylpolysiloxanes; D013440:Sulfides; C054170:dimethyl trisulfide; C013830:baysilon
keywords: Antidote; Cyanide; Dimethyl trisulfide; Gas chromatography mass spectrometry; Stir bar sorptive extraction