title: Metabolic engineering of Propionibacterium freudenreichii subsp. shermanii for xylose fermentation.
abstract: Propionibacterium freudenreichii cannot use xylose, the second most abundant sugar in lignocellulosic biomass. Although Propionibacterium acidipropionici can use xylose as a carbon source, it is difficult to genetically modify, impeding further improvement through metabolic engineering. This study identified three xylose catabolic pathway genes encoding for xylose isomerase (xylA), xylose transporter (xylT), and xylulokinase (xylB) in P. acidipropionici and overexpressed them in P. freudenreichii subsp. shermanii via an expression plasmid pKHEM01, enabling the mutant to utilize xylose efficiently even in the presence of glucose without glucose-induced carbon catabolite repression. The mutant showed similar fermentation kinetics with glucose, xylose, and the mixture of glucose and xylose, respectively, as carbon source, and with or without the addition of antibiotic for selection pressure. The engineered P. shermanii thus can provide a novel cell factory for industrial production of propionic acid and other value-added products from lignocellulosic biomass.
authors.affiliation: School of Biological and Chemical Engineering, Zhejiang University of Science & Technology, Hangzhou, Zhejiang 310023, China; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH 43210, USA.
authors.forename: Peilian
authors.initials: P
authors.lastname: Wei
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH 43210, USA; Bioprocessing Innovative Company, 4734 Bridle Path Ct., Dublin, OH 43017, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Lin
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH 43210, USA.
authors.forename: Zhongqiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH 43210, USA.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Fu
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH 43210, USA.
authors.forename: Hopen
authors.initials: H
authors.lastname: Yang
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH 43210, USA.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Jiang
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH 43210, USA. Electronic address: yang.15@osu.edu.
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D060847:Metabolic Engineering; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D000070002:Propionibacterium freudenreichii / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes; D014994:Xylose / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D014994:Xylose; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase
keywords: Catabolite repression; Metabolic engineering; Propionibacterium acidipropionici; Propionibacterium shermanii; Propionic acid; Xylose