title: Assessment of the Anti-anginal Effect of Tetramethylpyrazine Using Vasopressin-Induced Angina Model Rats.
abstract: Intravenous tetramethylpyrazine has been widely used in China as a complementary and/or alternative medicine to treat patients with ischemic heart disease. We assessed the anti-anginal effect of tetramethylpyrazine (10 mg/kg, intravenously (i.v.), n=6) in comparison with that of its vehicle, saline (1 mL/kg, i.v., n=6), using vasopressin-induced angina model rats. First, Donryu rats were anesthetized with pentobarbital sodium (60 mg/kg, intraperitoneally (i.p.)), and the surface lead I electrocardiogram was continuously monitored. Administration of vasopressin (0.5 IU/kg, i.v.) to the rats depressed the S-wave level of the electrocardiogram, indicating the onset of subendocardial ischemia. However, pretreatment with tetramethylpyrazine suppressed the vasopressin-induced depression of the S-wave level, which was not observed following pretreatment with its vehicle alone (saline), suggesting that tetramethylpyrazine ameliorated the vasopressin-induced subendocardial ischemia in vivo. These results may provide experimental evidence for the empirically known clinical efficacy of tetramethylpyrazine against ischemic heart disease, and could provide clues to better understanding its in vivo mechanism of action.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Toho University.
authors.forename: Xin
authors.initials: X
authors.lastname: Cao
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Wada
authors.forename: Takuya
authors.initials: T
authors.lastname: Kishie
authors.forename: Azjargal
authors.initials: A
authors.lastname: Enkhsaikhan
authors.forename: Hiroko
authors.initials: H
authors.lastname: Izumi-Nakaseko
authors.forename: Kentaro
authors.initials: K
authors.lastname: Ando
authors.forename: Bingmei
authors.initials: B
authors.lastname: Zhu
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sugiyama
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002316:Cardiotonic Agents / Q000627:therapeutic use*; D004195:Disease Models, Animal; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D011719:Pyrazines / Q000627:therapeutic use*; D051381:Rats; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents / Q000627:therapeutic use*; D014667:Vasopressins
chemical_list: D002316:Cardiotonic Agents; D011719:Pyrazines; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; D014667:Vasopressins; C017953:tetramethylpyrazine