title: Leishmania tarentolae as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6.
abstract: This chapter includes a practical method of membrane protein production in Leishmania tarentolae cells. We routinely use it to express membrane proteins of the ABC (adenosine triphosphate-binding cassette) family, here exemplified with ABCG6 from L. braziliensis, implicated in phospholipid trafficking and drug efflux. The pLEXSY system used here allows membrane protein production with a mammalian-like N-glycosylation pattern, at high levels and at low costs. Also the effects of an N-terminal truncation of the protein are described. The method is described to allow any kind of membrane protein production.
authors.affiliation: Drug Resistance and Membrane Proteins Team, UMR CNRS-UCBL1 5086, IBCP, 7, passage du Vercors, 69367, Lyon, France.
authors.forename: Lucia
authors.initials: L
authors.lastname: Gonzalez-Lobato
authors.affiliation: Drug Resistance and Membrane Proteins Team, UMR CNRS-UCBL1 5086, IBCP, 7, passage du Vercors, 69367, Lyon, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Chaptal
authors.affiliation: Drug Resistance and Membrane Proteins Team, UMR CNRS-UCBL1 5086, IBCP, 7, passage du Vercors, 69367, Lyon, France.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Molle
authors.affiliation: Drug Resistance and Membrane Proteins Team, UMR CNRS-UCBL1 5086, IBCP, 7, passage du Vercors, 69367, Lyon, France. pierre.falson@ibcp.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Falson
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015870:Gene Expression; D006031:Glycosylation; D006801:Humans; D007891:Leishmania / Q000235:genetics / Q000254:growth & development*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D015800:Protozoan Proteins / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; D008565:Membrane Proteins; D015800:Protozoan Proteins
keywords: ABC transporters; Drug efflux; Leishmania tarentolae; Membrane protein expression