title: Nocardioides baekrokdamisoli sp. nov., isolated from soil of a crater lake.
abstract: A novel actinobacterial strain, B2-12T, was isolated from soil of a crater lake, Baekrokdam, Hallasan, Jeju, Republic of Korea. Cells of strain B2-12T were Gram-stain-positive, non-motile, non-spore-forming and coccoid to short-rod-shaped. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain B2-12T belonged to the genus Nocardioides and shared highest sequence similarity with 'Nocardioidespaucivorans' KIS31-44 (98.4 %). The predominant isoprenoid quinone was MK-8(H4). The major fatty acids of strain B2-12T were C16 : 1 (ω7c and/or ω6c), summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), C17 : 0 10-methyl, iso-C16 : 0, C16 : 0 and C17 : 1ω6c. The diagnostic diamino acid in the cell-wall peptidoglycan was ll-diaminopimelic acid. It contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the polar lipids. The DNA G+C content was 67.0 mol%. Based on phylogenetic, chemotaxonomic and phenotypic data, 16S rRNA gene sequence comparisons and DNA-DNA hybridization data, strain B2-12T represents a novel species in the genus Nocardioides, for which the name Nocardioidesbaekrokdamisolisp. nov. is proposed. The type strain is B2-12T (=KCTC 39748T=NRRL B-65313T=DSM 100725T).
authors.affiliation: Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea.
authors.forename: Keun Chul
authors.initials: KC
authors.lastname: Lee
authors.affiliation: Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea.
authors.forename: Kwang Kyu
authors.initials: KK
authors.lastname: Kim
authors.affiliation: Gyeongbuk Institute for Marine Bioindustry, Uljin 36315, Republic of Korea.
authors.forename: Jong-Shik
authors.initials: JS
authors.lastname: Kim
authors.affiliation: World Heritage and Mt. Hallasan Research Institute, Jeju Special Self-Governing Province 63341, Republic of Korea.
authors.forename: Dae-Shin
authors.initials: DS
authors.lastname: Kim
authors.affiliation: World Heritage and Mt. Hallasan Research Institute, Jeju Special Self-Governing Province 63341, Republic of Korea.
authors.forename: Suk-Hyung
authors.initials: SH
authors.lastname: Ko
authors.affiliation: World Heritage and Mt. Hallasan Research Institute, Jeju Special Self-Governing Province 63341, Republic of Korea.
authors.forename: Seung-Hoon
authors.initials: SH
authors.lastname: Yang
authors.affiliation: Semyung University, 579 Sinwoul-dong, Jecheon-si, Chungcheongbuk-do 27136, Republic of Korea.
authors.forename: Yong Kook
authors.initials: YK
authors.lastname: Shin
authors.affiliation: University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.|Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea.
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D060106:Lakes / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8; D003960:Diaminopimelic Acid