title: Evaluation of LIAISON® XL system for HBsAg, and anti-HCV and anti-HIV/Ag p24.
abstract: The aim of this study was to compare the data obtained using the new LIAISON® XL chemiluminescence system to search for HBsAg, anti-HCV, and anti-HIV1-2/p24 Ag with those obtained using the VITROS system currently adopted by the Microbiology Unit of the Hospital of Legnano. Routine samples of patients who were referred by practitioners for the determination of HBsAg (1,000 samples) and/or anti-HCV (1,002 samples) and/or anti-HIV1-2 (995 samples) were simultaneously analyzed using both systems. The concordant positive and discordant samples were re-examined for confirmation by means of an HBsAg neutralization assay, anti-HCV immunoblot, or anti-HIV1-2 Western blot; HBV-DNA, or HCV-RNA or HIV-RNA was also sought in the discordant samples. Samples of patients known to be positive were tested (100 HBsAg positive, 100 anti-HCV positive, and 100 HIV 1-2 positive) as well throughout treatment, with viremia levels becoming undetectable after treatment. The HBsAg, anti-HCV, and anti-HIV1-2 concordance between the two systems in routine series was respectively 99.8%, 98.5% and 99.7%, and 100% for all markers in samples known positive. The various molecular biology and confirmatory tests of the discordant samples were all negative (except for one anti-HCV positive sample). Measure of Cohen's kappa coefficient for HBsAg, anti-HCV, and anti-HIV gave K values of respectively 0.992, 0.946, and 0.980. In conclusion, the performance of the LIAISON® XL system in the routine laboratory determination for all three markers was comparable with that of the VITROS system. J. Med. Virol. 89:489-496, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Microbiology Unit, ASST Ovest Milanese, Hospital of Legnano, Milan, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: De Paschale
authors.affiliation: Microbiology Unit, ASST Ovest Milanese, Hospital of Legnano, Milan, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Manco
authors.affiliation: Microbiology Unit, ASST Ovest Milanese, Hospital of Legnano, Milan, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Belvisi
authors.affiliation: Microbiology Unit, ASST Ovest Milanese, Hospital of Legnano, Milan, Italy.
authors.forename: Debora
authors.initials: D
authors.lastname: Cagnin
authors.affiliation: Microbiology Unit, ASST Ovest Milanese, Hospital of Legnano, Milan, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Cerulli
authors.affiliation: Microbiology Unit, ASST Ovest Milanese, Hospital of Legnano, Milan, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Paganini
authors.affiliation: Microbiology Unit, ASST Ovest Milanese, Hospital of Legnano, Milan, Italy.
authors.forename: Olivia
authors.initials: O
authors.lastname: Arpino
authors.affiliation: Microbiology Unit, ASST Ovest Milanese, Hospital of Legnano, Milan, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Cianflone
authors.affiliation: Microbiology Unit, ASST Ovest Milanese, Hospital of Legnano, Milan, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Agrappi
authors.affiliation: Microbiology Unit, ASST Ovest Milanese, Hospital of Legnano, Milan, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Mirri
authors.affiliation: Microbiology Unit, ASST Ovest Milanese, Hospital of Legnano, Milan, Italy.
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Clerici
mesh_terms: D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000175:diagnosis*; D006509:Hepatitis B / Q000175:diagnosis*; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006526:Hepatitis C / Q000175:diagnosis*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D008163:Luminescent Measurements; D012698:Serologic Tests / Q000379:methods*
chemical_list: D015483:HIV Antibodies; D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies
keywords: hepatitis B virus; hepatitis C virus; human immunodeficiency virus