title: Conjugated linoleic acid rat pretreatment reduces renal damage in ischemia/reperfusion injury: Unraveling antiapoptotic mechanisms and regulation of phosphorylated mammalian target of rapamycin.
abstract: Conjugated linoleic acids (CLAs) are dietary components with beneficial effects on human health. The aim of this study was to evaluate the potential benefits of CLA pretreatment in a rat model of renal ischemia/reperfusion injury (IRI).
  
  
  
  Animals were treated with CLAs (200 mg/kg/day) or water for two weeks prior to sham surgery or to surgery to induce IRI. Renal function, oxidative stress, apoptosis, and cell proliferation markers, were evaluated. Moreover, kidney sections were submitted to histological evaluation. IRI induced increased serum creatinine, blood urea nitrogen, fractional sodium excretion, malondialdehyde, Bax, and phosphorylated mammalian target of rapamycin (P-mTOR), and decreased clearance of creatine, superoxide dismutase and catalase activities, and Bax in comparison with control groups. CLA prefeeding restored, at least in part, the above reported markers to normal levels, increased the anti-apoptotic protein, B-cell lymphoma 2 (Bcl-2), and reduce the histological damage.
  
  
  
  The results suggest that the decreased renal tissue damage and improved renal function and oxidative stress, in rats pretreated with CLAs before renal IRI induction, could be associated with downregulation of Bax and P-mTOR, and upregulation of Bcl-2. CLAs pretreatment resulted to protect against IRI through the regulation of signaling pathways involved in apoptosis.
authors.affiliation: Minimally Invasive Surgery Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Gholamali
authors.initials: G
authors.lastname: Javedan
authors.affiliation: Department of Nutrition, School of Health, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Farzad
authors.initials: F
authors.lastname: Shidfar
authors.affiliation: Department of Clinical Nutrition and Dietetic, National Institute and Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Sayed Hossein
authors.initials: SH
authors.lastname: Davoodi
authors.affiliation: Department of Food and Nutrition Policy and Planning Research, National Nutrition and Food Technology Research Institute, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Marjan
authors.initials: M
authors.lastname: Ajami
authors.affiliation: Physiology Research Center and Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Fazel
authors.initials: F
authors.lastname: Gorjipour
authors.affiliation: Research Group on Community Nutrition and Oxidative Stress, University of Balearic Islands, and CIBERobn (Physiopathology of Obesity and Nutrition CB12/03/30038), Palma de Mallorca, Spain.
authors.forename: Antoni
authors.initials: A
authors.lastname: Sureda
authors.affiliation: Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Nabavi
authors.affiliation: Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology Section, University of Pavia, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Daglia
authors.affiliation: Physiology Research Center and Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Pazoki-Toroudi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D007674:Kidney Diseases / Q000209:etiology / Q000517:prevention & control*; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy*; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000097:blood; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D044243:Linoleic Acids, Conjugated; D051028:bcl-2-Associated X Protein; D008315:Malondialdehyde; D003404:Creatinine; D013482:Superoxide Dismutase; C546845:mTOR protein, rat; D058570:TOR Serine-Threonine Kinases
keywords: Apoptosis; Conjugated linoleic acids; Oxidative stress; Renal function; Renal ischemia; Reperfusion injury