title: Extracts from plastrum testudinis reverse glucocorticoid-induced spinal osteoporosis of rats via targeting osteoblastic and osteoclastic markers.
abstract: Extracts from plastrum testudinis (PTE), an important traditional Chinese medicine, have been demonstrated promotion of osteoblastic function in vitro. This study aims to investigate the protective effect of PTE on glucocorticoid-induced osteoporosis(GIOP) in vivo and analyze therapeutic targets of PTE on GIOP. SD rats were randomly assigned to two experiments: preventive and therapeutic experiments, in which rats respectively received oral PTE at the same time of glucocorticoid injection or after glucocorticoid injection inducing osteoporosis. BMD, microarchitecture, biomechanics, bone metabolism markers and histomorphology were evaluated. mRNA and protein expression of OPG, Runx2, CTSK and MMP9 were examined.Results showed bone quality and bone quantity were significantly elevated by PTE. Histomorphometry showed thicker and denser bone trabecularsand more osteoblasts and less osteoclasts in group of PTE intervention. The mRNA expression of OPG was significantly upregulated whereas expression of CTSK was significantly downregulatedin different groups of PTE intervention. Stronger immunostaining for Runx2 and weaker immunostaining for CTSK were observed in groups of PTE intervention. This demonstrated that PTE may reverse GIOP in prevention and management via targeting OPG, Runx2 and CTSK in mRNA and protein levels.
authors.affiliation: Department of Spinal Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China; Laboratory Affiliated to National Key Discipline of Orthopaedic and Traumatology of Chinese Medicine, Guangzhou University of Chinese Medicine,Guangzhou 510405, China. Electronic address: 513469239@qq.com.
authors.forename: De
authors.initials: D
authors.lastname: Liang
authors.affiliation: The First School of Clinic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China. Electronic address: renhuispine@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Ren
authors.affiliation: The First School of Clinic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Qiu
authors.affiliation: The First School of Clinic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
authors.forename: Gengyang
authors.initials: G
authors.lastname: Shen
authors.affiliation: The First School of Clinic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xie
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
authors.forename: Qiushi
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Department of Spinal Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
authors.forename: Zhensong
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Department of Spinal Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Tang
authors.affiliation: The First School of Clinic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
authors.forename: Zhida
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Spinal Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China; Laboratory Affiliated to National Key Discipline of Orthopaedic and Traumatology of Chinese Medicine, Guangzhou University of Chinese Medicine,Guangzhou 510405, China. Electronic address: spinedrjxb@sina.com.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001696:Biomechanical Phenomena / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005938:Glucocorticoids / Q000009:adverse effects*; D007150:Immunohistochemistry; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism*; D010024:Osteoporosis / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013131:Spine / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014020:Tissue Extracts / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D005938:Glucocorticoids; D010936:Plant Extracts; D012333:RNA, Messenger; D014020:Tissue Extracts; C000613899:plastrum testudinis
keywords: Extracts from plastrum testudinis; Glucocorticoid-induced osteoporosis; Management; Mechanism; Prevention; Rat