title: New technique for fertilizing eggs of burbot, asp and ide under hatchery conditions.
abstract: The development of a new protocol for egg fertilization may increase embryo survival and benefit the aquaculture process. In the present study, a new technique of partially adding sperm to activated eggs in the artificial fertilization of burbot (Lota lota), ide (Leuciscus idus) and asp (Aspius aspius) eggs was evaluated. If the same volume of sperm was divided into two or three parts and added to eggs in 30-60s intervals, it significantly improved embryo survival at the eyed-egg-stage of development. In the present study, the periodic addition of spermatozoa to eggs affected fertilization (ide and asp) and embryo survival rates (ide, asp and burbot) and might be successfully applied under hatchery conditions.
authors.affiliation: Department of Lake and River Fisheries, Warmia and Mazury University, Olsztyn, Poland. Electronic address: darekk@uwm.edu.pl.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Kucharczyk
authors.affiliation: Department of Lake and River Fisheries, Warmia and Mazury University, Olsztyn, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Nowosad
authors.affiliation: Department of Ichthyology, Inland Fisheries Institute in Olsztyn, Poland.
authors.forename: Marek J
authors.initials: MJ
authors.lastname: Łuczyński
authors.affiliation: Department of Lake and River Fisheries, Warmia and Mazury University, Olsztyn, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Targońska
mesh_terms: D000818:Animals; D017756:Aquaculture; D047108:Embryonic Development; D005399:Fishes / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D012098:Reproduction / Q000502:physiology*
keywords: Artificial fertilization; Burbot; Cyprinids; Embryo survival