title: Rheological, mechanical and degradable properties of injectable chitosan/silk fibroin/hydroxyapatite/glycerophosphate hydrogels.
abstract: Silk fibroin (SF) and hydroxyapatite (HA) were incorporated into chitosan/glycerophosphate (GP) system to prepare new types of hydrogels. The formulated chitosan/SF/GP and chitosan/SF/HA/GP solutions were found to be injectable at room temperature, and able to form into hydrogels at near-physiological temperature and pH. Rheological measurements showed that elastic modulus of certain chitosan/SF/GP and chitosan/SF/HA/GP gels could reach around 1.8 and 15kPa, respectively, and was much higher than their respective viscous modulus. Compressive measurements revealed that some chitosan/SF/GP and chitosan/SF/HA/GP gels had 8 and 20-fold modulus and strength higher than the chitosan/GP gel, respectively, confirming that compressive properties of these gels were greatly improved. Results obtained from in vivo degradation demonstrated that degradation endurance of the optimized chitosan/SF/GP and chitosan/SF/HA/GP gels was significantly enhanced as compared to the chitosan/GP gel, and the degradation rate of the gels could be regulated by the SF component alone or by the combination of SF and HA components.
authors: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
authors: Jingjing
authors: J
authors: Wu
authors: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
authors: Jiaoyan
authors: J
authors: Liu
authors: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
authors: Yanmei
authors: Y
authors: Shi
authors: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China. Electronic address: ying_x_wan@yahoo.ca.
authors: Ying
authors: Y
authors: Wan
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D005994:Glycerophosphates / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D012212:Rheology
chemical_list: D005994:Glycerophosphates; D020100:Hydrogels; D005349:Fibroins; D048271:Chitosan; D017886:Durapatite
keywords: Characterization and property; Chitosan; Hydroxyapatite; Injectable hydrogel; Silk fibroin