title: MicroRNA Let-7b inhibits keratinocyte migration in cutaneous wound healing by targeting IGF2BP2.
abstract: Wound healing is a complex process which involves proliferation and migration of keratinocyte for closure of epidermal injuries. A member of microRNA family, let-7b, has been expressed in mammalian skin, but its exact role in keratinocyte migration is still not in knowledge. Here, we showed that let-7b regulates keratinocyte migration by targeting the insulin-like growth factor IGF2BP2. Overexpression of let-7b led to reduced HaCaT cell migration, while knockdown of let-7b resulted in enhanced migration. Furthermore, let-7b was decreased during wound healing in wild-type mice, which led us to construct the transgenic mice with overexpression of let-7b in skin. The re-epithelialization of epidermis of let-7b transgenic mice was reduced during wound healing. Using bioinformatics prediction software and a reporter gene assay, we found that IGF2BP2 was a target of let-7b, which contributes to keratinocyte migration. Introduction of an expression vector of IGF2BP2 also rescued let-7b-induced migration deficiency, which confirms that IGF2BP2 is an important target for let-7b regulation. Our findings suggest that let-7b significantly delayed the re-epithelialization possibly due to reduction of keratinocyte migration and restraints IGF2BP2 during skin wound healing.
authors: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, China.|111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering & Key Laboratory of Biorheological Science and Technology, Ministry of Education Chongqing University, Chongqing, China.|College of Life Sciences, Jiangsu University, Zhenjiang, China.
authors: Yan
authors: Y
authors: Wu
authors: 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering & Key Laboratory of Biorheological Science and Technology, Ministry of Education Chongqing University, Chongqing, China.
authors: Julia Li
authors: JL
authors: Zhong
authors: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, China.
authors: Ning
authors: N
authors: Hou
authors: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, China.
authors: Yaolan
authors: Y
authors: Sun
authors: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, China.
authors: Benting
authors: B
authors: Ma
authors: 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering & Key Laboratory of Biorheological Science and Technology, Ministry of Education Chongqing University, Chongqing, China.
authors: Muhammad Farrukh
authors: MF
authors: Nisar
authors: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, China.
authors: Yan
authors: Y
authors: Teng
authors: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, China.
authors: Zhaoli
authors: Z
authors: Tan
authors: College of Life Sciences, Jiangsu University, Zhenjiang, China.
authors: Keping
authors: K
authors: Chen
authors: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, China.
authors: Youliang
authors: Y
authors: Wang
authors: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, China.
authors: Xiao
authors: X
authors: Yang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics*; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D015603:Keratinocytes / Q000502:physiology; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D062326:Re-Epithelialization / Q000235:genetics*; D012879:Skin Physiological Phenomena
chemical_list: D020413:3' Untranslated Regions; C498779:IGF2BP2 protein, human; C559768:IGF2BP2 protein, mouse; D035683:MicroRNAs; D016601:RNA-Binding Proteins; C487931:mirnlet7 microRNA, human; C542687:mirnlet7 microRNA, mouse
keywords: IGF2BP2; keratinocyte; microRNA let-7b; migration; wound healing