title: Structure elucidation, proliferation effect on macrophage and its mechanism of a new heteropolysaccharide from Lactarius deliciosus Gray.
abstract: A new heteropolysaccharide was isolated from the fruiting bodies of Lactarius deliciosus Gray which had a molecular weight of 16kDa and was mainly composed of the galactose and glucose. Structural elucidation results indicated that Lactarius deliciosus Gray polysaccharide (LDG-B) had a backbone of (1,6)-linked d-galactose and (1, 2, 6)-linked d-galactose which branches were mainly composed of 4-linked d-glucose and 6-linked d-galactose residue. Cell cycle test results showed that LDG-B could promote the proliferation of B cells and macrophage cells by affecting G0/G1 phase, S phases and G2/M phases. The analysis of transcriptomes sequence of macrophages showed a total of 1839 genes were identified as DEGs, and approximately 708 genes were up-regulated, whereas 1131 genes were down-regulated in LDG-B group. KEGG pathway enrichment analysis showed that the MAPK, JAK-STAT and NF-κB signaling pathways are significantly enriched for DEGs in LDG-B group. Analysis of transcriptome resources enabled us to examine gene expression profiles, verify differential gene expression, and select candidate signaling pathways as the mechanisms of the immunomodulatory activity of LDG-B.
authors: Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, 1# Shida Road, Nanchong, Sichuan Province 637009, China.
authors: Yiling
authors: Y
authors: Hou
authors: Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, 1# Shida Road, Nanchong, Sichuan Province 637009, China.
authors: Lu
authors: L
authors: Liu
authors: Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, 1# Shida Road, Nanchong, Sichuan Province 637009, China; College of Environmental Science and Engineering, China West Normal University, 1# Shida Road, Nanchong, Sichuan Province 637009, China. Electronic address: biostart8083@126.com.
authors: Xiang
authors: X
authors: Ding
authors: Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, 1# Shida Road, Nanchong, Sichuan Province 637009, China.
authors: Daqun
authors: D
authors: Zhao
authors: Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, 1# Shida Road, Nanchong, Sichuan Province 637009, China.
authors: Wanru
authors: W
authors: Hou
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002453:Cell Cycle / Q000187:drug effects*; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D000067996:RAW 264.7 Cells; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D062610:Fungal Polysaccharides
keywords: Immunomodulatory activity; Macrophage; Polysaccharide; RNA-seq; Structure