title: Development of a preparation method to produce a single sample that can be applied to both LC-MS/MS and GC-MS for the screening of postmortem specimens.
abstract: Simple and efficient extraction methods have been developed for the screening of a wide array of drugs in postmortem autopsy specimens. Acidic and basic compounds were targeted with two extraction methods that can be applied to both GC-MS and LC-MS/MS instrumentation. The extractions were achieved by utilizing lipid-removal and solid-phase extraction cartridges while carefully monitoring the pH of the samples to ensure the adequate removal of interfering substances like lipids and amino acid derivatives. These methods were applied to actual autopsy cases, with 94 and 124 compounds detected by GC-MS and LC-MS/MS, respectively. The developed methods could easily be incorporated into a forensic laboratory's daily routine for screening many different compounds from postmortem samples.
authors: Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Japan.
authors: Kenji
authors: K
authors: Hara
authors: Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Japan.
authors: Brian
authors: B
authors: Waters
authors: Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Japan.
authors: Natsuki
authors: N
authors: Ikematsu
authors: Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Japan.
authors: Tomoko
authors: T
authors: Tokuyasu
authors: Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Japan.
authors: Hiroshi
authors: H
authors: Fujii
authors: Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Japan.
authors: Mio
authors: M
authors: Takayama
authors: Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Japan.
authors: Aya
authors: A
authors: Matsusue
authors: Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Japan.
authors: Masayuki
authors: M
authors: Kashiwagi
authors: Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Japan. Electronic address: kuboshin@fukuoka-u.ac.jp.
authors: Shin-Ichi
authors: S
authors: Kubo
mesh_terms: D001344:Autopsy; D002853:Chromatography, Liquid; D053593:Forensic Toxicology / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013048:Specimen Handling / Q000379:methods*; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
keywords: Forensic autopsy; GC–MS; LC–MS/MS; Sample preparation; Toxicological screening