title: Differential microRNA Expression and Regulation in the Rat Model of Post-Infarction Heart Failure.
abstract: BACKGROUND
  Heart failure is a complex end stage of various cardiovascular diseases with a poor prognosis, and the mechanisms for development and progression of heart failure have always been a hot point. However, the molecular mechanisms underlying the post transcriptional regulation of heart failure have not been fully elucidated. Current data suggest that microRNAs (miRNAs) are involved in the pathogenesis of heart failure and could serve as a new biomarker, but the precise regulatory mechanisms are still unclear.
  
  
  METHODS
  The differential miRNA profile in a rat model of post-infarction heart failure was determined using high throughout sequencing and analyzed through bioinformatics approaches. The results were validated using qRT-PCR for 8 selected miRNAs. Then the expression patterns of 4 miRNAs were analyzed in different periods after myocardial infarction. Finally, gain- and loss-of-function experiments of rno-miR-122-5p and rno-miR-184 were analyzed in H2O2 treated H9c2 cells.
  
  
  RESULTS
  In the heart failure sample, 78 miRNAs were significantly upregulated and 28 were downregulated compared to the controls. GO and KEGG pathway analysis further indicated the likely roles of these miRNAs in heart failure. Time-course analysis revealed different expression patterns of 4 miRNAs: rno-miR-122-5p, rno-miR-199a-5p, rno-miR-184 and rno-miR-208a-3p. Additionally, rno-miR-122-5p and rno-miR-184 were proved to promote apoptosis in vitro.
  
  
  CONCLUSIONS
  Differential profile and expression patterns of miRNAs in the rats model of post-infarction heart failure were found, and the pro-apoptotic roles of rno-miR-122-5p and rno-miR-184 were revealed. These findings may provide a novel way that may assist in heart failure diagnosis and treatment.
authors: Department of Internal Medicine and Cardiology, China-Japan Union Hospital of Jilin University, Changchun, China.
authors: Xueyan
authors: X
authors: Liu
authors: Clinical Medicine, Yanbian University, Yanji, China.
authors: Heyu
authors: H
authors: Meng
authors: Department of Hepatobiliary Pancreatic Surgery, First Hospital of Jilin University, Changchun, China.
authors: Chao
authors: C
authors: Jiang
authors: Department of Internal Medicine and Cardiology, China-Japan Union Hospital of Jilin University, Changchun, China.
authors: Sibao
authors: S
authors: Yang
authors: Department of Internal Medicine and Cardiology, China-Japan Union Hospital of Jilin University, Changchun, China.
authors: Fengwen
authors: F
authors: Cui
authors: Department of Internal Medicine and Cardiology, China-Japan Union Hospital of Jilin University, Changchun, China.
authors: Ping
authors: P
authors: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D006333:Heart Failure / Q000150:complications* / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D058977:Molecular Sequence Annotation; D009203:Myocardial Infarction / Q000150:complications*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D035683:MicroRNAs