title: Identification and characteristic analysis of the catalase gene from Locusta migratoria.
abstract: Catalase (CAT) is a ubiquitous antioxidant enzyme in almost all living organisms exposed to atmosphere, which involved in decomposing harmful hydrogen peroxide, into oxygen and water. In this study, a full-length cDNA (1524bp) encoding the catalase gene (LmCAT) from Locusta migratoria was cloned (accession number KT716445). The open reading frame of the LmCAT gene encoded 507 amino acids and shared 57.8%-97.8% amino acid identities with other insect CATs. The coding region was interrupted by 9 introns, while its promoter region contained 15 putative binding sites for 5 kinds of transcriptional regulation factors. For the stage-specific expression profile, LmCAT was highly expressed in the fourth-instar nymphs. For the tissue-specific expression profile, the LmCAT transcripts were highest in the fat bodies, and relatively abundant in the gastric caecum, Malpighian tubules, ovary and integument. Moreover, the result showed that quercetin could significantly induce the expression level of LmCAT. The expression of LmCAT could be silenced by RNAi, but the moralities were not significantly different between control and RNAi groups. Our results would provide valuable information for further study on the ROS regulation mechanism in insect.
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors: Xueyao
authors: X
authors: Zhang
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors: Yahong
authors: Y
authors: Li
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors: Junxiu
authors: J
authors: Wang
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors: Tingting
authors: T
authors: Zhang
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors: Tao
authors: T
authors: Li
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors: Wei
authors: W
authors: Dong
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China. Electronic address: maenbo2003@sxu.edu.cn.
authors: Enbo
authors: E
authors: Ma
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China. Electronic address: zjz@sxu.edu.cn.
authors: Jianzhen
authors: J
authors: Zhang
mesh_terms: D000818:Animals; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D049750:Genome, Insect / Q000235:genetics; D048188:Locusta migratoria / Q000201:enzymology / Q000235:genetics*; D009758:Nymph / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D000072416:Protein Structural Elements; D034622:RNA Interference; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics
chemical_list: D014157:Transcription Factors; D002374:Catalase
keywords: Catalase; Locusta migratoria; RT-qPCR