title: Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy.
abstract: In many vertebrate cell types, the proximal part of the primary cilium is positioned within an invagination of the plasma membrane known as the ciliary pocket. Recent evidence points to the conclusion that the ciliary pocket comprises a unique site for exocytosis and endocytosis of ciliary proteins, which regulates the spatiotemporal trafficking of receptors into and out of the cilium to control its sensory function. In this chapter, we provide methods based on electron microscopy, 3D reconstruction of fluorescence images as well as live cell imaging suitable for investigating processes associated with endocytosis at the ciliary pocket.
authors: Centre de Recherche en Cancérologie de Marseille (CRCM), Inserm, U1068-CNRS UMR7258, Institut Paoli-Calmettes, Aix-Marseille Université, 13009, Marseille, France.
authors: Rania
authors: R
authors: Ghossoub
authors: Department of Biology, University of Copenhagen, Universitetsparken 13, DK-2100, Copenhagen, OE, Denmark.
authors: Louise
authors: L
authors: Lindbæk
authors: Department of Genetics and Developmental Biology, Institut Curie, Paris, France.|CNRS, UMR, Paris, France.
authors: Anahi
authors: A
authors: Molla-Herman
authors: INSERM, U1016, Institut Cochin, 75014, Paris, France.|CNRS, UMR8104, 75014, Paris, France.|Université Paris Descartes, Sorbonne Paris Cité, 75006, Paris, France.
authors: Alain
authors: A
authors: Schmitt
authors: Department of Biology, University of Copenhagen, Universitetsparken 13, DK-2100, Copenhagen, OE, Denmark. stchristensen@bio.ku.dk.
authors: Søren Tvorup
authors: ST
authors: Christensen
authors: INSERM, UMR-1163, Laboratory of Inherited Kidney Diseases, 75015, Paris, France. alexandre.benmerah@inserm.fr.|Université Paris Descartes-Sorbonne Paris Cité, Imagine Institut, 75015, Paris, France. alexandre.benmerah@inserm.fr.
authors: Alexandre
authors: A
authors: Benmerah
mesh_terms: D002460:Cell Line; D002923:Cilia / Q000378:metabolism* / Q000648:ultrastructure*; D002966:Clathrin / Q000378:metabolism; D022163:Clathrin-Coated Vesicles / Q000378:metabolism; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D021621:Imaging, Three-Dimensional; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D038081:Organogenesis / Q000235:genetics; D021381:Protein Transport; D055213:Retinal Pigment Epithelium; D015398:Signal Transduction; D014168:Transferrin / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D002966:Clathrin; D014168:Transferrin; D053773:Transforming Growth Factor beta1
keywords: Cellular signaling; Ciliary pocket; Clathrin-dependent endocytosis; Early endosomes; Primary cilia