title: Supplementation with nanomolar concentrations of verbascoside during in vitro maturation improves embryo development by protecting the oocyte against oxidative stress: a large animal model study.
abstract: The effects of verbascoside (VB), added at nanomolar concentrations during in vitro maturation (IVM) of juvenile sheep oocytes, on in vitro embryo development and its mechanisms of action at the oocyte level were analyzed. Developmental rates, after IVM in the presence/absence of VB (1nM for 24h; 1nM for 2h; 10nM for 2h), were evaluated. The bioenergetic/oxidative status of oocytes matured after IVM in the presence/absence of 1nM VB for 24h was assessed by confocal analysis of mitochondria and reactive oxygen species (ROS), lipid peroxidation (LPO) assay, and quantitative PCR of bioenergy/redox-related genes. The addition of 1nM VB during 24h IVM significantly increased blastocyst formation and quality. Verbascoside reduced oocyte ROS and LPO and increased mitochondria/ROS colocalization while keeping mitochondria activity and gene expression unchanged. In conclusion, supplementation with nanomolar concentrations of VB during IVM, in the juvenile sheep model, promotes embryo development by protecting the oocyte against oxidative stress.
authors: Department of Biosciences, Biotechnologies and Biopharmaceutics (DBBB), University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy; Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Via Manfredonia 20, 71121 Foggia, Italy. Electronic address: nicmartino@libero.it.
authors: Nicola Antonio
authors: NA
authors: Martino
authors: Obstetrics and Gynecological Section, Department of Veterinary Medicine, Via Vienna, n°2 07100 Sassari, Italy. Electronic address: federica@uniss.it.
authors: Federica
authors: F
authors: Ariu
authors: Obstetrics and Gynecological Section, Department of Veterinary Medicine, Via Vienna, n°2 07100 Sassari, Italy. Electronic address: dbebbere@uniss.it.
authors: Daniela
authors: D
authors: Bebbere
authors: Department of Biosciences, Biotechnologies and Biopharmaceutics (DBBB), University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy. Electronic address: m.filioliuranio@gmail.com.
authors: Manuel Filioli
authors: MF
authors: Uranio
authors: Department of Biosciences, Biotechnologies and Biopharmaceutics (DBBB), University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy. Electronic address: adriana.chirico@gmail.com.
authors: Adriana
authors: A
authors: Chirico
authors: Department of Biosciences, Biotechnologies and Biopharmaceutics (DBBB), University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy. Electronic address: ros.marzano@gmail.com.
authors: Giuseppina
authors: G
authors: Marzano
authors: Department of Basical Medical Sciences, Neurosciences and Sensory Organs, University of Bari Aldo Moro, P.zza G. Cesare, 11, 70124 Bari, Italy; Institute of Translational Pharmacology (IFT), National Research Council of Italy (CNR) via del Fosso del Cavaliere 100, 00133 Rome, Italy. Electronic address: annamaria.sardanelli@uniba.it.
authors: Anna Maria
authors: AM
authors: Sardanelli
authors: Institute of Sciences of Food Production (ISPA), National Research Council of Italy (CNR), Via G. Amendola 122/O, 70125 Bari, Italy. Electronic address: angela.cardinali@ispa.cnr.it.
authors: Angela
authors: A
authors: Cardinali
authors: Institute of Sciences of Food Production (ISPA), National Research Council of Italy (CNR), Via G. Amendola 122/O, 70125 Bari, Italy. Electronic address: fiorenza.minervini@ispa.cnr.it.
authors: Fiorenza
authors: F
authors: Minervini
authors: Obstetrics and Gynecological Section, Department of Veterinary Medicine, Via Vienna, n°2 07100 Sassari, Italy. Electronic address: luis@uniss.it.
authors: Luisa
authors: L
authors: Bogliolo
authors: Department of Biosciences, Biotechnologies and Biopharmaceutics (DBBB), University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy. Electronic address: mariaelena.dellaquila@uniba.it.
authors: Maria Elena
authors: ME
authors: Dell'Aquila
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects; D046149:Embryo Culture Techniques / Q000662:veterinary; D047108:Embryonic Development / Q000187:drug effects*; D005307:Fertilization in Vitro / Q000662:veterinary; D005960:Glucosides / Q000494:pharmacology*; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D015227:Lipid Peroxidation / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012756:Sheep; D059808:Polyphenols
chemical_list: D005960:Glucosides; D010636:Phenols; D020011:Protective Agents; D017382:Reactive Oxygen Species; C058956:acteoside; D059808:Polyphenols
keywords: Blastocyst development; IVM; Juvenile sheep oocyte; Oocyte bioenergetics and oxidative stress; Verbascoside