title: Region of Nipah virus C protein responsible for shuttling between the cytoplasm and nucleus.
abstract: Nipah virus (NiV) causes severe encephalitis in humans, with high mortality. NiV nonstructural C protein (NiV-C) is essential for its pathogenicity, but its functions are unclear. In this study, we focused on NiV-C trafficking in cells and found that it localizes predominantly in the cytoplasm but partly in the nucleus. An analysis of NiV-C mutants showed that amino acids 2, 21-24 and 110-139 of NiV-C are important for its localization in the cytoplasm. Inhibitor treatment indicates that the nuclear export determinant is not a classical CRM1-dependent nuclear export signal. We also determined that amino acids 60-75 and 72-75 were important for nuclear localization of NiV-C. Furthermore, NiV-C mutants that had lost their capacity for nuclear localization inhibited the interferon (IFN) response more strongly than complete NiV-C. These results indicate that the IFN-antagonist activity of NiV-C occurs in the cytoplasm.
authors: Laboratory Animal Research Center and International Research Center for Infectious Diseases, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, Japan.
authors: Ryo
authors: R
authors: Horie
authors: Laboratory Animal Research Center and International Research Center for Infectious Diseases, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, Japan. Electronic address: yone@ims.u-tokyo.ac.jp.
authors: Misako
authors: M
authors: Yoneda
authors: Laboratory Animal Research Center and International Research Center for Infectious Diseases, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, Japan.
authors: Shotaro
authors: S
authors: Uchida
authors: Laboratory Animal Research Center and International Research Center for Infectious Diseases, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, Japan.
authors: Hiroki
authors: H
authors: Sato
authors: Laboratory Animal Research Center and International Research Center for Infectious Diseases, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, Japan.
authors: Chieko
authors: C
authors: Kai
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism* / Q000821:virology*; D017124:Conserved Sequence; D003593:Cytoplasm / Q000378:metabolism* / Q000821:virology*; D015870:Gene Expression; D017930:Genes, Reporter; D045464:Henipavirus Infections / Q000821:virology; D006801:Humans; D045405:Nipah Virus / Q000502:physiology*; D019913:Nuclear Localization Signals; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*; D014779:Virus Replication
chemical_list: C543302:C protein, Nipah virus; D019913:Nuclear Localization Signals; D010750:Phosphoproteins; D014764:Viral Proteins
keywords: C protein; Nipah virus; Nuclear export signal; Nuclear localization signal; Subcellular localization