title: Elucidating the pH-Dependent Structural Transition of T7 Bacteriophage Endolysin.
abstract: Bacteriophages are the most abundant and diverse biological entities on earth. Bacteriophage endolysins are unique peptidoglycan hydrolases and have huge potential as effective enzybiotics in various infectious models. T7 bacteriophage endolysin (T7L), also known as N-acetylmuramoyl-l-alanine amidase or T7 lysozyme, is a 17 kDa protein that lyses a range of Gram-negative bacteria by hydrolyzing the amide bond between N-acetylmuramoyl residues and the l-alanine of the peptidoglycan layer. Although the activity profiles of several of the T7 family members have been known for many years, the molecular basis for their pH-dependent differential activity is not clear. In this study, we explored the pH-induced structural, stability, and activity characteristics of T7L by applying a variety of biophysical techniques and protein nuclear magnetic resonance (NMR) spectroscopy. Our studies established a reversible structural transition of T7L below pH 6 and the formation of a partially denatured conformation at pH 3. This low-pH conformation is thermally stable and exposed its hydrophobic pockets. Further, NMR relaxation measurements and structural analysis unraveled that T7L is highly dynamic in its native state and a network of His residues are responsible for the observed pH-dependent conformational dynamics and transitions. As bacteriophage chimeric and engineered endolysins are being developed as novel therapeutics against multiple drug resistance pathogens, we believe that our results are of great help in designing these entities as broadband antimicrobial and/or antibacterial agents.
authors: Meenakshi
authors: M
authors: Sharma
authors: Centre of Biomedical Research, SGPGIMS , Lucknow 226014, Uttar Pradesh, India.
authors: Dinesh
authors: D
authors: Kumar
authors: Krishna Mohan
authors: KM
authors: Poluri
mesh_terms: D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000737:chemistry*; D011487:Protein Conformation; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D009238:N-Acetylmuramoyl-L-alanine Amidase