title: Correction of Microplate Data from High-Throughput Screening.
abstract: High-throughput screening (HTS) makes it possible to collect cellular response data from a large number of cell lines and small molecules in a timely and cost-effective manner. The errors and noises in the microplate-formatted data from HTS have unique characteristics, and they can be generally grouped into three categories: run-wise (temporal, multiple plates), plate-wise (background pattern, single plate), and well-wise (single well). In this chapter, we describe a systematic solution for identifying and correcting such errors and noises, mainly basing on pattern recognition and digital signal processing technologies.
authors: National Center for Advancing Translational Sciences, National Institutes of Health, Building B, MSC: 3370, 9800 Medical Center Drive, Bethesda, MD, 20892, USA. wangyuh@mail.nih.gov.
authors: Yuhong
authors: Y
authors: Wang
authors: National Center for Advancing Translational Sciences, National Institutes of Health, Building B, MSC: 3370, 9800 Medical Center Drive, Bethesda, MD, 20892, USA.
authors: Ruili
authors: R
authors: Huang
mesh_terms: D000465:Algorithms; D016477:Artifacts; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D057166:High-Throughput Screening Assays / Q000592:standards*; D006801:Humans; D010363:Pattern Recognition, Automated / Q000592:standards*; D015203:Reproducibility of Results; D059629:Signal-To-Noise Ratio; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: D015262:Xenobiotics
keywords: Concentration response; Data correction; HTS; In vitro assay