title: [Micronucleus in vitro induced by inhalable particulate matters in moxa smoke].
abstract: OBJECTIVE
  To investigate whether inhalable particulate matters can cause the damage of chromosome or mitotic apparatus to produce micronucleus, and to evaluate genetic toxicology of moxa smoke on chromosome.
  
  
  METHODS
  By MTT method, the 24 h half maximal inhibitory concentration (IC50) of moxa smoke condensation (MSC) on Chinese hamster ovary (CHO) cells was 0.087 mg/mL. CHO cells, which were cultured in vitro, were divided into a solvent control group, a positive control group (cyclophosphamide as solvent), a low concentration group, a moderate concentration group and a high concentration group. The low concentration group, moderate concentration group and high concentration group were set approximately 1/8, 1/4, 1/2 of IC50, respectively. Whether micronucleus had dose-effect response induced by the damage of chromosome or mitotic apparatus was observed after CHO cells were contaminated by MSC in the low concentration group, moderate concentration group and high concentration group.
  
  
  RESULTS
  The rate of micronucleus induced by MSC in the low concentration group, moderate concentration group and high concentration group was higher than that in the solvent control group (all P < 0.05), which presented dosage-effect response. The experiment was repeated 3 times, indicating it was repeatable with statistical significance.
  
  
  CONCLUSIONS
  High concentration of MSC shows toxicity to induce chromosome damage, which disappears at low concentration. The genetic toxicology is also dependent on concentration, and the concentration of moxa smoke is essential. In clinical treatment, it is noted to control the level of moxa smoke, while the clinical safety standard of moxa smoke concentration is in need of further study.
authors: Li
authors: L
authors: Han
authors: Hai
authors: H
authors: Hu
authors: Jia
authors: J
authors: Yang
authors: Hua
authors: H
authors: Bai
authors: Lei
authors: L
authors: Wang
authors: Juntian
authors: J
authors: Liu
authors: Chang
authors: C
authors: Huang
authors: Yaomeng
authors: Y
authors: Liu
authors: Lue
authors: L
authors: Ha
mesh_terms: D000393:Air Pollutants / Q000009:adverse effects*; D000818:Animals; D016466:CHO Cells; D002467:Cell Nucleus / Q000187:drug effects* / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D019570:Inhalation Exposure / Q000009:adverse effects* / Q000032:analysis; D015162:Micronucleus Tests; D009071:Moxibustion / Q000009:adverse effects*; D052638:Particulate Matter / Q000009:adverse effects*; D012906:Smoke / Q000009:adverse effects* / Q000032:analysis
chemical_list: D000393:Air Pollutants; D052638:Particulate Matter; D012906:Smoke