title: Metaproteomics reveals metabolic transitions between healthy and diseased stony coral Mussismilia braziliensis.
abstract: Infectious diseases such as white plague syndrome (WPS) and black band disease (BBD) have caused massive coral loss worldwide. We performed a metaproteomic study on the Abrolhos coral Mussismilia braziliensis to define the types of proteins expressed in healthy corals compared to WPS- and BBD-affected corals. A total of 6363 MS/MS spectra were identified as 361 different proteins. Healthy corals had a set of proteins that may be considered markers of holobiont homoeostasis, including tubulin, histone, Rab family, ribosomal, peridinin-chlorophyll a-binding protein, F0F1-type ATP synthase, alpha-iG protein, calmodulin and ADP-ribosylation factor. Cnidaria proteins found in healthy M. braziliensis were associated with Cnidaria-Symbiodinium endosymbiosis and included chaperones (hsp70, hsp90 and calreticulin), structural and membrane modelling proteins (actin) and proteins with functions related to intracellular vesicular traffic (Rab7 and ADP-ribosylation factor 1) and signal transduction (14-3-3 protein and calmodulin). WPS resulted in a clear shift in the predominance of proteins, from those related to aerobic nitrogen-fixing bacteria (i.e. Rhizobiales, Sphingomonadales and Actinomycetales) in healthy corals to those produced by facultative/anaerobic sulphate-reducing bacteria (i.e. Enterobacteriales, Alteromonadales, Clostridiales and Bacteroidetes) in WPS corals. BBD corals developed a diverse community dominated by cyanobacteria and sulphur cycle bacteria. Hsp60, hsp90 and adenosylhomocysteinase proteins were produced mainly by cyanobacteria in BBD corals, which is consistent with elevated oxidative stress in hydrogen sulphide- and cyanotoxin-rich environments. This study demonstrates the usefulness of metaproteomics for gaining better comprehension of coral metabolic status in health and disease, especially in reef systems such as the Abrolhos that are suffering from the increase in global and local threatening events.
authors: Instituto de Biologia, Universidade Federal do Rio de Janeiro (UFRJ), Av. Carlos Chagas Fo. SN., Ilha do Fundão, Rio de Janeiro, RJ, CEP 21941-902, Brasil.
authors: Gizele D
authors: GD
authors: Garcia
authors: Divisão de Metrologia Aplicada às Ciências da Vida (DIMAV), Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO), Av. Nossa Senhora das Graças, 50, Xerém Duque de Caxias, Rio de Janeiro, RJ, CEP 25250-020, Brasil.|Unidade de Biologia, Centro Universitário Estadual da Zona Oeste (UEZO), Av. Manoel Caldeira de Alvarenga, 1203, Campo Grande, Rio de Janeiro, RJ, CEP 23070200, Brasil.
authors: Eidy de O
authors: Ede O
authors: Santos
authors: Divisão de Metrologia Aplicada às Ciências da Vida (DIMAV), Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO), Av. Nossa Senhora das Graças, 50, Xerém Duque de Caxias, Rio de Janeiro, RJ, CEP 25250-020, Brasil.
authors: Gabriele V
authors: GV
authors: Sousa
authors: Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro (UFRJ), Av. Carlos Chagas Fo. SN, Ilha do Fundão, Rio de Janeiro, RJ, CEP21941-902, Brasil.
authors: Russolina B
authors: RB
authors: Zingali
authors: Instituto de Biologia, Universidade Federal do Rio de Janeiro (UFRJ), Av. Carlos Chagas Fo. SN., Ilha do Fundão, Rio de Janeiro, RJ, CEP 21941-902, Brasil.
authors: Cristiane C
authors: CC
authors: Thompson
authors: Instituto de Biologia, Universidade Federal do Rio de Janeiro (UFRJ), Av. Carlos Chagas Fo. SN., Ilha do Fundão, Rio de Janeiro, RJ, CEP 21941-902, Brasil. fabianothompson1@gmail.com.|Laboratório de Sistemas Avançados de Gestão da Produção (SAGE), COPPE, Universidade Federal do Rio de Janeiro (UFRJ), Rua Moniz de Aragão, no.360 - Bloco 2, Ilha do Fundão - Cidade Universitária, Rio de Janeiro, RJ, 21.941-972, Brasil. fabianothompson1@gmail.com.
authors: Fabiano L
authors: FL
authors: Thompson
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000235:genetics* / Q000382:microbiology*; D001419:Bacteria / Q000145:classification*; D001938:Brazil; D040901:Proteomics; D013559:Symbiosis; D053719:Tandem Mass Spectrometry
keywords: Abrolhos Bank; Mussismilia braziliensis; black band disease; metaproteome; white plague syndrome