title: Direct PCR of Intact Bacteria (Colony PCR).
abstract: This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using the polymerase chain reaction (PCR). This method is commonly referred to as colony PCR. © 2016 by John Wiley & Sons, Inc.
authors: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky.|Present address: Michael E. Woodman, Eli Lilly and Company, Indianapolis, Indiana.
authors: Michael E
authors: ME
authors: Woodman
authors: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky.
authors: Christina R
authors: CR
authors: Savage
authors: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky.
authors: William K
authors: WK
authors: Arnold
authors: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky.
authors: Brian
authors: B
authors: Stevenson
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004269:DNA, Bacterial
keywords: bacteria; colony PCR; screen