title: Nelumbinis Semen reverses a decrease in 5-HT1A receptor binding induced by chronic mild stress, a depression-like symptom.
abstract: Depression is associated with a dysfunctional serotonin (5-hydroxytryptamine; 5-HT) system. More recently, several lines of evidence suggest that an important factor in the development of depression may be a deficit in the function and expression of 5-HT1A receptors. The present study assessed ifNelumbinis Semen (N. s.) had an anti-depression effect through reversing a decrease in 5-HT1A receptor binding in rats with depression-like symptoms induced by chronic mild stress. Using a 5-HT1A receptor binding assay, with a specific 5-HT1A receptor agonist, 8-OH-DPAT (8-hydroxy-2-(di-n-propylamino) tetralin), the mechanism of the anti-depression effect ofN. s. on rats was investigated, and the effects compared with two well-known anti-depressants,Hyperium Perforatum (St. Johns Wort) and fluoxetine (Prozac). Animals were divided into five groups: the normal (N) group without chronic mild stress (CMS), the control (C) group under CMS for 8 weeks, theNelumbinis Semen (N. s.) treatment group under CMS for 8 weeks, theHyperium Perforatum (H. p.) treatment group under CMS for 8 weeks and finally, the fluoxetine (F) treatment group under CMS for 8 weeks. Each treatment was administered to rats during the last 4 weeks of the 8-week CMS. A sucrose intake test was performed to test the anti-depression effect ofN. s. TheN. s. treatment significantly reversed the decreased sucrose intake under CMS (P<0.05 compared to control group under CMS). In the CA2 and CA3 regions of the hippocampus, bothN. s. andH. p. reversed the CMS-induced decrease in 5-HT1A receptor binding. In the I to II regions of the frontal cortex,N. s. andH. p. also reversed the CMS-induced decrease in 5-HT1A receptor binding, and even showed a significant increase in 5-HT1A receptor binding compared to the F treatment group (N. s. vs. P, p<0.05,H. p. vs. P, p<0.05). However, in the hypothalamus, all treatments reversed the CMS-induced decrease in 5-HT1A receptor binding. This reversal effect ofN. s. on the decrease in 5-HT1A receptor binding in the frontal cortex, hippocampus and hypothalamus of rat brains was very similar to that ofH. p, but different from that of F. It is concluded thatN. s. presents an anti-depression effect through enhancing 5-HT1A receptor binding.
authors: Department of Pharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea.
authors: Choon-Gon
authors: CG
authors: Jang
authors: Department of Pharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea.|Purimed R&D Institute, Kyunghee University, 130-701, Hoeki-Dong, Dongdaemoon-Ku, Seoul, Korea.
authors: Moonkyu
authors: M
authors: Kang
authors: Department of Pharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea.
authors: Jae-Han
authors: JH
authors: Cho
authors: Department of Pharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea.
authors: Sun-Bok
authors: SB
authors: Lee
authors: Department of Pharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea.|Department of Psychology, Korea University, 136-701, Seoul, Korea.
authors: Hyuntaek
authors: H
authors: Kim
authors: Department of Pharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea.|Department of Anatomy, Korea University College of Medicine, 136-705, Seoul, Korea.
authors: Soonkwon
authors: S
authors: Park
authors: Department of Pharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea.|Department of Physiology, College of Oriental Medicine, Kyunghee University, 130-701, Seoul, Korea.
authors: Jinwoo
authors: J
authors: Lee
authors: Department of Pharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea.|Department of Physiology, College of Oriental Medicine, Kyunghee University, 130-701, Seoul, Korea.
authors: Seong-Kyu
authors: SK
authors: Park
authors: Department of Pharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea.|Department of Physiology, College of Oriental Medicine, Kyunghee University, 130-701, Seoul, Korea.
authors: Moochang
authors: M
authors: Hong
authors: Department of Pharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea.|Department of Physiology, College of Oriental Medicine, Kyunghee University, 130-701, Seoul, Korea.
authors: Min Kyu
authors: MK
authors: Shin
authors: Department of Pharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea.|East-West Medicine Research Institute, Kyunghee University, 449-701, Kyunggi-Do, Suwon, Korea.
authors: In-Sup
authors: IS
authors: Shim
authors: Department of Pharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea. hbae@khu.ac.kr.|Purimed R&D Institute, Kyunghee University, 130-701, Hoeki-Dong, Dongdaemoon-Ku, Seoul, Korea. hbae@khu.ac.kr.|Department of Physiology, College of Oriental Medicine, Kyunghee University, 130-701, Seoul, Korea. hbae@khu.ac.kr.
authors: Hyunsu
authors: H
authors: Bae
keywords: 5-HT1A receptor; Anti-depression; Chronic mild stress; Dysfunctional 5-HT system; Nelumbinis Semen