title: Fine mapping of a dominant gene conferring chlorophyll-deficiency in Brassica napus.
abstract: Leaf colour regulation is important in photosynthesis and dry material production. Most of the reported chlorophyll-deficient loci are recessive. The dominant locus is rarely reported, although it may be more important than the recessive locus in the regulation of photosynthesis efficiency. During the present study, we mapped a chlorophyll-deficient dominant locus (CDE1) from the ethyl methanesulfonate-mutagenized Brassica napus line NJ7982. Using an F2 population derived from the chlorophyll-deficient mutant (cde1) and the canola variety 'zhongshuang11', a high-density linkage map was constructed, consisting of 19 linkage groups with 2,878 bins containing 13,347 SNP markers, with a total linkage map length of 1,968.6 cM. Next, the CDE1 locus was mapped in a 0.9-cM interval of chromosome C08 of B. napus, co-segregating with nine SNP markers. In the following fine-mapping of the gene using the inherited F2:3 populations of 620 individuals, the locus was identified in an interval with a length of 311 kb. A bioinformatics analysis revealed that the mapping interval contained 22 genes. These results produced a good foundation for continued research on the dominant locus involved in chlorophyll content regulation.
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors: Yankun
authors: Y
authors: Wang
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors: Yongjun
authors: Y
authors: He
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors: Mao
authors: M
authors: Yang
authors: Soybean Research Institute, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors: Jianbo
authors: J
authors: He
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors: Pan
authors: P
authors: Xu
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors: Mingquan
authors: M
authors: Shao
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors: Pu
authors: P
authors: Chu
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors: Rongzhan
authors: R
authors: Guan
mesh_terms: D029688:Brassica napus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002734:Chlorophyll / Q000172:deficiency; D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics; D005020:Ethyl Methanesulfonate / Q000009:adverse effects; D009154:Mutation; D010788:Photosynthesis; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide
chemical_list: D010940:Plant Proteins; D002734:Chlorophyll; D005020:Ethyl Methanesulfonate