title: Disinfection of human skin allografts in tissue banking: a systematic review report.
abstract: The use of skin allografts to temporarily replace lost or damaged skin is practiced worldwide. Naturally occurring contamination can be present on skin or can be introduced at recovery or during processing. This contamination can pose a threat to allograft recipients. Bacterial culture and disinfection of allografts are mandated, but the specific practices and methodologies are not dictated by standards. A systematic review of literature from three databases found 12 research articles that evaluated bioburden reduction processes of skin grafts. The use of broad spectrum antibiotics and antifungal agents was the most frequently identified disinfection method reported demonstrating reductions in contamination rates. It was determined that the greatest reduction in the skin allograft contamination rates utilized 0.1 % peracetic acid or 25 kGy of gamma irradiation at lower temperatures.
authors: Capital Health Regional Tissue Bank, 5788 University Avenue, Room 431 Mackenzie Building, Halifax, NS, B3H 1V7, Canada.
authors: C
authors: C
authors: Johnston
authors: Sunnybrook Health Sciences Centre Blood and Tissue Bank, 2075 Bayview Ave., Room B2 04, Toronto, ON, M4N 3M5, Canada.
authors: J
authors: J
authors: Callum
authors: Canadian Blood Services, 270 John Savage Ave., Dartmouth, NS, B3B 0H7, Canada.
authors: J
authors: J
authors: Mohr
authors: Department of Surgery, McMaster University, 293 Wellington St. N, Suite 110, Hamilton, ON, L8L 8E7, Canada.
authors: A
authors: A
authors: Duong
authors: Department of Surgery, McMaster University, 293 Wellington St. N, Suite 110, Hamilton, ON, L8L 8E7, Canada.
authors: A
authors: A
authors: Garibaldi
authors: Department of Surgery, McMaster University, 293 Wellington St. N, Suite 110, Hamilton, ON, L8L 8E7, Canada.
authors: N
authors: N
authors: Simunovic
authors: Department of Surgery, McMaster University, 293 Wellington St. N, Suite 110, Hamilton, ON, L8L 8E7, Canada. Ayenif@mcmaster.ca.|McMaster University Medical Centre, 1200 Main St W, Room 4E15, Hamilton, ON, L8N 3Z5, Canada. Ayenif@mcmaster.ca.
authors: O R
authors: OR
authors: Ayeni
mesh_terms: D064591:Allografts / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods; D004203:Disinfection / Q000379:methods*; D006801:Humans; D012867:Skin / Q000382:microbiology*; D016038:Skin Transplantation / Q000009:adverse effects / Q000379:methods; D013242:Sterilization / Q000379:methods*; D014015:Tissue Banks; D014184:Transplantation, Homologous
keywords: Bioburden; Skin allografts; Skin decontamination; Tissue banking; Tissue donation