title: Ibuprofen-loaded porous microspheres suppressed the progression of monosodium iodoacetate-induced osteoarthritis in a rat model.
abstract: The objectives of this study were (1) to fabricate ibuprofen-loaded porous microspheres (IBU/PMSs), (2) to evaluate the in vitro anti-inflammatory effects of the microspheres using LPS-induced inflammation in cultured synoviocytes, and (3) to evaluate the in vivo effect of the IBU/PMSs on the progression of monosodium iodoacetate (MIA)-induced osteoarthritis (OA) in a rat model. A dose-dependent in vitro anti-inflammatory effect on pro-inflammatory cytokine markers (matrix metallopeptidase-3 (MMP-3), matrix metallopeptidase-13 (MMP-13), cyclooxygenase-2 (COX-2), a disintegrin and metalloproteinase with thrombospondin motifs-5 (ADAMTS-5)), interleukin-6 (IL-6), and tumor necrosis factor (TNF-α) was observed by confirming with real-time PCR analyses. In vivo, treatment with IBU/PMSs reduced MIA-stimulated mRNA expression of MMP-3, MMP-13, COX-2, ADAMTS-5, IL-6, and TNF-α in rat synoviocytes. In addition, we demonstrated that intra-articular IBU/PMSs suppressed the progression of MIA-induced OA in the rat model via anti-inflammatory mechanisms. In conclusion, IBU/PMSs are a promising therapeutic material to control the pain and progression of OA.
authors: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul, 152-703 Republic of Korea.
authors: Jang Won
authors: JW
authors: Park
authors: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul, 152-703 Republic of Korea.
authors: Young-Pil
authors: YP
authors: Yun
authors: Division of Biological Imaging, Chuncheon Center, Korea Basic Science Institute, 192-1 Hyoja 2-dong, Chuncheon, Gangwon-do 200-701 Republic of Korea.
authors: Kyeongsoon
authors: K
authors: Park
authors: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul, 152-703 Republic of Korea; Department of Biomedical Science, College of Medicine, Korea University, Anam-dong, Seongbuk-gu, 136-701 Republic of Korea.
authors: Jae Yong
authors: JY
authors: Lee
authors: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul, 152-703 Republic of Korea.
authors: Hak-Jun
authors: HJ
authors: Kim
authors: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul, 152-703 Republic of Korea. Electronic address: sekim10@korea.ac.kr.
authors: Sung Eun
authors: SE
authors: Kim
authors: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul, 152-703 Republic of Korea. Electronic address: songhae@korea.ac.kr.
authors: Hae-Ryong
authors: HR
authors: Song
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D049109:Cell Proliferation / Q000187:drug effects*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000633:toxicity; D007052:Ibuprofen / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000378:metabolism; D019807:Iodoacetic Acid / Q000633:toxicity*; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000235:genetics; D008863:Microspheres; D010003:Osteoarthritis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D004791:Enzyme Inhibitors; D018836:Inflammation Mediators; D014409:Tumor Necrosis Factor-alpha; D051546:Cyclooxygenase 2; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; D019807:Iodoacetic Acid; D007052:Ibuprofen
keywords: Anti-inflammatory effect; Ibuprofen; Osteoarthritis; PLGA; Porous microspheres