title: Phospholipase C catalyzed formation of sphingomyelin-(14)C from lecithin and N-((14)C)-oleoyl-sphingosine.
abstract: Commercial preparations ofClostridium perfringens were incubated with phosphatidyl choline and N-1-((14)C) oleoylsphingosine. A radioactive product was formed which cochromatogramed with sphingomyelin standard in three different solvent systems. Several other phospholipases and phosphatases were unable to catalyze this reaction. Neither choline, phosphoryl choline, cytidine diphosphate choline nor p-nitrophenyl phosphoryl choline were active donors. Sphingomyelin was only slightly active as a phosphoryl choline donor.
authors: Eunice Kennedy Shriver Center at the W.E. Fernald State School, 02154, Waltham, Massachusetts.
authors: J N
authors: JN
authors: Kanfer
authors: Eunice Kennedy Shriver Center at the W.E. Fernald State School, 02154, Waltham, Massachusetts.
authors: C H
authors: CH
authors: Spievlogel