title: Efficacy of pegylated liposomal etoposide nanoparticles on breast cancer cell lines.
abstract: OBJECTIVE
  This study aimed to investigate the efficacy of pegylated liposomal etoposide nanoparticles (NPs) against T-47D and MCF-7 breast cancer cell lines.
  
  
  METHODS
  Pegylated liposomal etoposide NPs were prepared by reverse phase evaporation method. The size, size distribution, and zeta potential of the NPs was measured by a Zetasizer instrument. The cytotoxicity of NPs was inspected by methyl thiazol tetrazolium assay. The release pattern of the drug from the vesicles was studied by the dialysis method. Drug loading and encapsulation efficiency (EE) were also measured.
  
  
  RESULTS
  The mean size, size distribution, and zeta potential of pegylated liposomal etoposide NPs were 491 ± 15.5 nm, 0.504 ± 0.14, and -35.8 ± 2.5 mV, respectively. Drug loading and EE were 10.3 ± 1.6% and 99.1 ± 2.8%, respectively. The etoposide release in the formulation was estimated at about 3.48% after 48 h. The cytotoxicity effect of etoposide NPs on T-47D and MCF-7 cell lines of breast cancer showed higher antitumor activity as compared with those of the free drug.
  
  
  CONCLUSIONS
  Liposome-based NPs may hold great potential as a drug delivery system.
authors: Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors: Mohammadreza
authors: M
authors: Mehrabi
authors: Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors: Parisa
authors: P
authors: Esmaeilpour
authors: Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors: Azim
authors: A
authors: Akbarzadeh
authors: Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors: Zahra
authors: Z
authors: Saffari
authors: Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors: Maryam
authors: M
authors: Farahnak
authors: Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors: Ali
authors: A
authors: Farhangi
authors: Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors: Mohsen
authors: M
authors: Chiani
mesh_terms: D001943:Breast Neoplasms; D005047:Etoposide; D006801:Humans; D008081:Liposomes; D061986:MCF-7 Cells; D053758:Nanoparticles
chemical_list: D008081:Liposomes; D005047:Etoposide
keywords: Etoposide; breast cancer; cytotoxicity; pegylated liposomal NPs