title: Interrelationship of Primary Virus Replication, Level of Latency, and Time to Reactivation in the Trigeminal Ganglia of Latently Infected Mice.
abstract: We sought to determine the possibility of an interrelationship between primary virus replication in the eye, the level of viral DNA in the trigeminal ganglia (TG) during latency, and the amount of virus reactivation following ocular herpes simplex virus type 1 (HSV-1) infection. Mice were infected with virulent (McKrae) or avirulent (KOS and RE) strains of HSV-1, and virus titers in the eyes and TG during primary infection, level of viral gB DNA in TG on day 28 postinfection (p.i.), and virus reactivation on day 28 p.i. as measured by explant reactivation were calculated. Our results suggest that the avirulent strains of HSV-1, even after corneal scarification, had lower virus titers in the eye, had less latency in the TG, and took a longer time to reactivate than virulent strains of HSV-1. The time to explant reactivation of avirulent strains of HSV-1 was similar to that of the virulent LAT((-)) McKrae-derived mutant. The viral dose with the McKrae strain of HSV-1 affected the level of viral DNA and time to explant reactivation. Overall, our results suggest that there is no absolute correlation between primary virus titer in the eye and TG and the level of viral DNA in latent TG and time to reactivation.
  
  
  
  Very little is known regarding the interrelationship between primary virus replication in the eye, the level of latency in TG, and the time to reactivate in the mouse model. This study was designed to answer these questions. Our results point to the absence of any correlation between the level of primary virus replication and the level of viral DNA during latency, and neither was an indicator of how rapidly the virus reactivated following explant TG-induced reactivation.
authors: Center for Neurobiology and Vaccine Development, Ophthalmology Research Laboratory, and Department of Surgery, Cedars-Sinai Burns & Allen Research Institute, Los Angeles, California, USA.
authors: Harry H
authors: HH
authors: Matundan
authors: Center for Neurobiology and Vaccine Development, Ophthalmology Research Laboratory, and Department of Surgery, Cedars-Sinai Burns & Allen Research Institute, Los Angeles, California, USA.
authors: Kevin R
authors: KR
authors: Mott
authors: Center for Neurobiology and Vaccine Development, Ophthalmology Research Laboratory, and Department of Surgery, Cedars-Sinai Burns & Allen Research Institute, Los Angeles, California, USA.
authors: Sariah J
authors: SJ
authors: Allen
authors: Center for Neurobiology and Vaccine Development, Ophthalmology Research Laboratory, and Department of Surgery, Cedars-Sinai Burns & Allen Research Institute, Los Angeles, California, USA.
authors: Shaohui
authors: S
authors: Wang
authors: Biostatistics & Bioinformatics Core at the Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Burns & Allen Research Institute, Los Angeles, California, USA.
authors: Catherine J
authors: CJ
authors: Bresee
authors: Zilkha Neurogenetic Institute, Department of Physiology & Biophysics, and Program in Global Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.
authors: Yasamin N
authors: YN
authors: Ghiasi
authors: Zilkha Neurogenetic Institute, Department of Physiology & Biophysics, and Program in Global Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.
authors: Terrence
authors: T
authors: Town
authors: The Gavin Herbert Eye Institute and Department of Ophthalmology, University of California, Irvine, School of Medicine, Irvine, California, USA.
authors: Steven L
authors: 0000-0001-8509-5229
authors: SL
authors: Wechsler
authors: Center for Neurobiology and Vaccine Development, Ophthalmology Research Laboratory, and Department of Surgery, Cedars-Sinai Burns & Allen Research Institute, Los Angeles, California, USA ghiasih@cshs.org.
authors: Homayon
authors: H
authors: Ghiasi
mesh_terms: D000818:Animals; D003315:Cornea / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D006561:Herpes Simplex / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D012668:Trigeminal Ganglion / Q000821:virology*; D019562:Viral Load / Q000379:methods; D014775:Virus Activation / Q000235:genetics*; D017735:Virus Latency / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics*
chemical_list: D004279:DNA, Viral