title: Haplotype Analysis of BRCA1 Gene D17S855 and D17S1322 Markers in Iranian Familial Breast Cancer Patients.
abstract: BACKGROUND
  Breast cancer molecular analysis by linkage analysis has the advantage of facilitating early diagnosis in asymptomatic genetic carriers, with a view to the preventive followup of these subjects and genetic counseling. The aim of this study was to evaluate BRCA1 gene D17S855 and D17S1322 markers in breast cancer patients.
  
  
  METHODS
  A series of 107 BC patients and 93 unrelated healthy women were recruited for haplotype analysis performed using two short tandem repeat markers located within the BRCA1 gene locus. Each marker was amplified with PCR genomic DNA from each individual and fluorescently endlabeled primers.
  
  
  RESULTS
  Both D17S855 and D17S1322 markers included 12 kinds of alleles. Results indicate that most of the BC patients shared two common 121150 (11.2%, RR=1.56 and p=0.02) and 121146 (5.6%, RR=1.9 and p=0.02) haplotypes.
  
  
  CONCLUSIONS
  Our results should be helpful to understand the haplotype phase in the BRCA1 gene and establish a genetic screening strategy in the Iranian population.
authors: Department of Biology, Science Faculty, University of Sistan and Baluchestan, Zahedan, Iran Email : hn_1364@yahoo.com.
authors: Sayed Mohsen
authors: SM
authors: Miresmaeili
authors: Dor Mohammad
authors: DM
authors: Kordi
authors: Seyed Mohammad
authors: SM
authors: Moshtaghiun
mesh_terms: D000328:Adult; D000483:Alleles; D019313:BRCA1 Protein / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005817:Genetic Counseling / Q000379:methods; D005820:Genetic Testing / Q000379:methods; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D007492:Iran; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D019313:BRCA1 Protein; D014408:Biomarkers, Tumor; D017931:DNA Primers