title: Interspecies variation of Kitasatospora recifensis endophytic from yam bean producing thermostable amylases in alternative media.
abstract: An endophytic actinomycete isolated from tubers of yam beam (Pachyrhizus erosus L. Urban) was classified as a novel species nominated Kitasatospora recifensis based in phenotypic and genotypic analysis (16S rDNA gene sequence). Monosporic culture using specific ISP2 media revealed three interspecies, which were identified by DNA southern hybridization (Wild strain 13817 W, Aerial Mycelium strain 13817 AM and Vegetative Mycelium strain 13817 VM). The strains were tested for the production of amylolitic enzymes in alternative media. Maximum yields for both enzymes were observed in starch-casein. Higher α-amylase was obtained with strain 13817 W in starch-urea, and amyloglucosidase with strain 13817 AM in starch-ammonium that are economic sources and may be important for industrial purposes. Type strain (DAUFPE 13817(T) = KCTC 9972(T )= DSM 44943(T)).
authors: Department of Nutrition, University Federal of Pernambuco-UFPE, Rua Jader de Andrade, 335 Casa Forte, 52061-060, Recife, Pernambuco, Brazil. tlmstamford@yahoo.com.br.
authors: Tania Lucia Montenegro
authors: TL
authors: Stamford
authors: University Integrated of Patos, Paraíba, Brazil.
authors: Thayza Christina Montenegro
authors: TC
authors: Stamford
authors: Department of Agronomy, University Federal Rural of Pernambuco-UFRPE, Recife, Brazil.
authors: Newton Pereira
authors: NP
authors: Stamford
authors: Department of Agronomy, University Federal Rural of Pernambuco-UFRPE, Recife, Brazil.
authors: Carolina Etienne Rosália Silva
authors: CE
authors: Santos
authors: Department of Agronomy, University Federal Rural of Pernambuco-UFRPE, Recife, Brazil.
authors: Maria do Carmo Catanho Pereira
authors: Mdo C
authors: de Lyra
authors: Korea Research Institute of Bioscience & Biotechnology, Dae Jeon, Korea.
authors: Yong
authors: Y
authors: Ha-Park
authors: Korea Research Institute of Bioscience & Biotechnology, Dae Jeon, Korea.
authors: Jin-Won
authors: JW
authors: Bae
authors: Department of Anitibiotics, University Federal of Pernambuco, Recife, Brazil.
authors: Janete Magali
authors: JM
authors: Araújo
keywords: 16S rDNA; Amyloglucosidase; Amylolitic enzymes; Gene sequence; Pachyrhizus erosus; α-Amylase