title: Generation and characterization of human B lymphocyte stimulator blocking monoclonal antibody.
abstract: The cytokine, B lymphocyte stimulator (Blys) is essential for activation and proliferation of B cells and is involved in the pathogenesis of B-cell mediated autoimmune diseases. Based on its essential activity, Blys may be a potential therapeutic target for human autoimmune diseases. In this article, we have described the development of a novel humanized anti-Blys antibody, NMB04, that binds with high affinity and specificity to both soluble and membrane bound Blys. This monoclonal antibody has the potential to block Blys binding to all its three receptors, TACI, BCMA and BR-3. Further in vivo studies revealed that NMB04 possessed more potent inhibitory activity against human Blys as compared to an existing antibody, Belimumab. Therefore, NMB04 may have potential as a therapeutic candidate targeting autoimmune diseases.
authors: Novomab Biopharmaceuticals Inc., Nanjing 210042, China.
authors: Weiliang
authors: W
authors: Zhuang
authors: China Pharmaceutical University, Nanjing 210009, China.
authors: Jianjun
authors: J
authors: Zhang
authors: Novomab Biopharmaceuticals Inc., Nanjing 210042, China.
authors: Lili
authors: L
authors: Pei
authors: Novomab Biopharmaceuticals Inc., Nanjing 210042, China.
authors: Shuping
authors: S
authors: Fang
authors: Novomab Biopharmaceuticals Inc., Nanjing 210042, China.
authors: Honghao
authors: H
authors: Liu
authors: China Pharmaceutical University, Nanjing 210009, China.
authors: Ruixue
authors: R
authors: Wang
authors: Novomab Biopharmaceuticals Inc., Nanjing 210042, China. Electronic address: suyunpeng@simcere.com.
authors: Yunpeng
authors: Y
authors: Su
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology*; D000915:Antibody Affinity / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D053264:B-Cell Activating Factor / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D008252:Macaca fascicularis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction
chemical_list: D000911:Antibodies, Monoclonal; D053264:B-Cell Activating Factor; C505719:TNFSF13B protein, human
keywords: Antibody; Autoimmune; Blys; SLE