title: Synthesis of tumor-targeted folate conjugated fluorescent magnetic albumin nanoparticles for enhanced intracellular dual-modal imaging into human brain tumor cells.
abstract: Superparamagnetic iron oxide nanoparticles (SPIO NPs), utilized as carriers are attractive materials widely applied in biomedical fields, but target-specific SPIO NPs with lower toxicity and excellent biocompatibility are still lacking for intracellular visualization in human brain tumor diagnosis and therapy. Herein, bovine serum albumin (BSA) coated superparamagnetic iron oxide, i.e. γ-Fe2O3 nanoparticles (BSA-SPIO NPs), are synthesized. Tumor-specific ligand folic acid (FA) is then conjugated onto BSA-SPIO NPs to fabricate tumor-targeted NPs, FA-BSA-SPIO NPs as a contrast agent for MRI imaging. The FA-BSA-SPIO NPs are also labeled with fluorescein isothiocyanate (FITC) for intracellular visualization after cellular uptake and internalization by glioma U251 cells. The biological effects of the FA-BSA-SPIO NPs are investigated in human brain tumor U251 cells in detail. These results show that the prepared FA-BSA-SPIO NPs display undetectable cytotoxicity, excellent biocompatibility, and potent cellular uptake. Moreover, the study shows that the made FA-BSA-SPIO NPs are effectively internalized for MRI imaging and intracellular visualization after FITC labeling in the targeted U251 cells. Therefore, the present study demonstrates that the fabricated FITC-FA-BSA-SPIO NPs hold promising perspectives by providing a dual-modal imaging as non-toxic and target-specific vehicles in human brain tumor treatment in future.
authors: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China. Electronic address: wangxq0708@163.com.
authors: Xueqin
authors: X
authors: Wang
authors: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
authors: Miaomiao
authors: M
authors: Tu
authors: School of Life Sciences, Zhengzhou University, Zhengzhou, Henan 450001, PR China.
authors: Baoming
authors: B
authors: Tian
authors: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
authors: Yanjie
authors: Y
authors: Yi
authors: School of Life Sciences, Zhengzhou University, Zhengzhou, Henan 450001, PR China.
authors: ZhenZhen
authors: Z
authors: Wei
authors: School of Life Sciences, Zhengzhou University, Zhengzhou, Henan 450001, PR China. Electronic address: fangwei@zzu.edu.cn.
authors: Fang
authors: F
authors: Wei
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism; D002417:Cattle; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000494:pharmacology; D005492:Folic Acid / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008422:Materials Testing; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000494:pharmacology
chemical_list: D003287:Contrast Media; D058185:Magnetite Nanoparticles; D012710:Serum Albumin, Bovine; D005492:Folic Acid; D016650:Fluorescein-5-isothiocyanate
keywords: Bovine serum albumin; Dual-modal imaging; Fluorescent magnetic nanoparticles; Folate; Human brain tumor