title: Pre-embedding Nanogold Silver and Gold Intensification.
abstract: Pre-embedding nanogold silver and gold intensification methods involve immunoreactions with nanogold-labeled antibodies and intensification of the nanogold particles before embedding and ultrathin sectioning. These highly sensitive methods show good resolution and ultrastructural preservation. They also are useful for simultaneous observation of immunolabeled cells under light and electron microscopes, and for three-dimensional immunoelectron microscopic analyses. Silver intensification is usually superior for immunolabeling. On the other hand, ultrastructural preservation is better when gold intensification is used. In this chapter, we introduce pre-embedding nanogold silver and gold intensification procedures for use primarily with cultured cells.
authors: Department of Animal Bio-Science, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-cho, Nagahama, Shiga, 526-0829, Japan. a_yamamoto@nagahama-i-bio.ac.jp.
authors: Akitsugu
authors: A
authors: Yamamoto
authors: Department of Animal Bio-Science, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-cho, Nagahama, Shiga, 526-0829, Japan.
authors: Ryuichi
authors: R
authors: Masaki
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D019556:COS Cells / Q000378:metabolism / Q000648:ultrastructure; D002522:Chlorocebus aethiops; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000648:ultrastructure; D004853:Epoxy Resins / Q000737:chemistry; D005404:Fixatives / Q000737:chemistry; D005557:Formaldehyde / Q000737:chemistry; D005615:Freezing; D015870:Gene Expression; D017979:Gold Colloid / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D050958:HSP47 Heat-Shock Proteins; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D016253:Microscopy, Immunoelectron / Q000379:methods*; D008867:Microtomy; D041681:NIH 3T3 Cells; D011108:Polymers / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D000906:Antibodies; D004853:Epoxy Resins; D005404:Fixatives; D017979:Gold Colloid; D050958:HSP47 Heat-Shock Proteins; D011108:Polymers; D049452:Green Fluorescent Proteins; D005557:Formaldehyde; D012834:Silver; D000444:Aldehyde Dehydrogenase; C003043:paraform
keywords: Cultured cells; Gold intensification; Nanogold; Pre-embedding immunoelectron microscopy; Silver intensification