title: eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients.
abstract: OBJECTIVE
  The purpose of the present study was to investigate whether endothelial nitric oxide synthase (eNOS) gene polymorphisms play a role in prostate cancer (PCa).
  
  
  METHODS
  We examined three eNOS gene polymorphisms (T-786C promoter region, G894T, and Intron 4 VNTR 4a/b) at extracted DNAs from 50 formalin-fixed paraffin-embedded tissues of PCa patients. For the controls, blood samples obtained from 50 healthy men were studied. Genotyping of molecular variants was performed by PCR-RFLP technique.
  
  
  RESULTS
  We found that the TC genotype of the T-786C polymorphism was associated with PCa risk (OR: 3.325, CI: 1.350-8.188, P = 0.008). The eNOS G894T polymorphism was also associated with PCa. The frequency of the 894T allele was significantly higher in PCa patients. No association was identified between intron 4 VNTR polymorphism and PCa.
  
  
  CONCLUSIONS
  We found significant differences in genotypic and allelic frequencies between PCa patients and controls for eNOS T-786C and G894T polymorphisms. The presence of the T-786C genotype and 894T allele in carriers increased the risk of PCa. No association was found between intron 4 VNTR polymorphism and PCa patients.
authors: Department of Primary Education, Faculty of Education, Elementary Sciences Education, Kocaeli University, Campus of Umuttepe, Kocaeli, Turkey.
authors: Fikriye
authors: F
authors: Polat
authors: Vocational School of Health Services, Selçuk University, Konya, Turkey.
authors: Nesrin
authors: N
authors: Turaçlar
authors: Department of Research Centre, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
authors: Meral
authors: M
authors: Yilmaz
authors: Biomedical Engineering, Faculty of Engineering and Natural Sciences, Yıldırım Beyazıt University, Ankara, Turkey.
authors: Günsel
authors: G
authors: Bingöl
authors: Department of Biology, Molecular Biology, Selçuk University, Selçuklu, Konya, Turkey.
authors: Hasibe
authors: H
authors: Cingilli Vural
mesh_terms: D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats; D052250:Nitric Oxide Synthase Type III; D016612:Paraffin Embedding; D011110:Polymorphism, Genetic; D011471:Prostatic Neoplasms
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: Polymorphism; eNOS; prostate cancer