title: HCV NS3 naturally occurring variants in HIV/HCV coinfected DAA-naïve patients: consideration for HCV genotyping resistance testing.
abstract: OBJECTIVE
  Data on the frequency of HCV naturally occurring drug-resistant variants (RAVs) at baseline in HIV/HCV coinfected patients are scarce.
  
  
  METHODS
  NS3-HCV RAVs were studied by full-population direct sequencing from plasma specimens of 345 DAA-naïve patients with HCV chronic hepatitis (159 of them with HIV/HCV-coinfection).
  
  
  RESULTS
  NS3 RAVs were identified in 31.5 % of patients, with a significant proportion of HIV/HCV coinfected DAA-naïve patients compared to those with HCV monoinfection (38 vs. 25 % p = 0.0104, OR 1.84; 95 % CI 1.162-2.916).
  
  
  CONCLUSIONS
  HCV resistance genotyping test before treatment may be worth in special populations such as HIV/HCV coinfection to optimize patient treatment.
authors: Laboratory of Microbiology and Virology, Department of Infectious Diseases, Amedeo di Savoia Hospital, Corso Svizzera 164, 10149, Turin, Italy. tinarugg@gmail.com.
authors: T
authors: T
authors: Ruggiero
authors: Laboratory of Microbiology and Virology, Department of Infectious Diseases, Amedeo di Savoia Hospital, Corso Svizzera 164, 10149, Turin, Italy.
authors: E
authors: E
authors: Burdino
authors: Unit of Infectious Diseases, Department of Medical Sciences, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.
authors: A
authors: A
authors: Calcagno
authors: Unit of Infectious Diseases, Department of Medical Sciences, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.
authors: S
authors: S
authors: Bonora
authors: Unit of Infectious Diseases, Department of Medical Sciences, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.
authors: L
authors: L
authors: Boglione
authors: Unit of Infectious Diseases, Department of Medical Sciences, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.
authors: G
authors: G
authors: Di Perri
authors: Laboratory of Microbiology and Virology, Department of Infectious Diseases, Amedeo di Savoia Hospital, Corso Svizzera 164, 10149, Turin, Italy.
authors: V
authors: V
authors: Ghisetti
mesh_terms: D000998:Antiviral Agents; D015331:Cohort Studies; D060085:Coinfection; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000150:complications*; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000150:complications* / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D000998:Antiviral Agents; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
keywords: DAAs; HIV/HCV coinfected; Hepatitis C virus; RAVs