title: Cu0.89Zn0.11O, A New Peroxidase-Mimicking Nanozyme with High Sensitivity for Glucose and Antioxidant Detection.
abstract: Nanomaterial-based enzyme mimetics (nanozymes) is an emerging field of research that promises to produce alternatives to natural enzymes for a variety of applications. The search for the most cost-effective and efficient inorganic nanomaterials, such as metal oxides, cannot be won by pristine CuO. However, unlike CuO, the Zn-doped CuO (Zn-CuO) nanoparticles reported in this paper reveal superior peroxidase-like enzyme activity. This places Zn-CuO in a good position to participate in a range of activities aimed at developing diverse enzyme applications. The peroxidase-like activity was tested and confirmed against various chromogenic substrates in the presence of H2O2 and obeyed the Michaelis-Menten enzymatic pathway. The mechanism of enhanced enzymatic activity was proved by employing terephthalic acid as a fluorescence probe and by electron spin resonance. The nanozyme, when tested for the detection of glucose, showed a substantial enhancement in the detection selectivity. The limit of detection (LOD) was also decreased reaching a limit as low as 0.27 ppm. Such a low LOD has not been reported so far for the metal oxides without any surface modifications. Moreover, the nanozyme (Zn-CuO) was utilized to detect the three antioxidants tannic acid, tartaric acid, and ascorbic acid and the relative strength of their antioxidant capacity was compared.
authors: Department of Chemistry and Institute for Nanotechnology and Advanced Materials, Bar-Ilan University , Ramat Gan 5290002, Israel.
authors: Anjani P
authors: AP
authors: Nagvenkar
authors: Department of Chemistry and Institute for Nanotechnology and Advanced Materials, Bar-Ilan University , Ramat Gan 5290002, Israel.
authors: Aharon
authors: A
authors: Gedanken
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D003300:Copper; D005947:Glucose / Q000032:analysis*; D006861:Hydrogen Peroxide; D009195:Peroxidase; D010544:Peroxidases; D015032:Zinc
chemical_list: D000975:Antioxidants; D003300:Copper; D006861:Hydrogen Peroxide; D010544:Peroxidases; D009195:Peroxidase; D005947:Glucose; D015032:Zinc
keywords: H2O2; Zn-CuO; antioxidant; dopant; glucose detection; nanozyme; peroxidase mimic