title: Nucleotide variation of sFRP5 gene is not associated with obesity in children and adolescents.
abstract: Because sFRP5 was shown to be an important extracellular modulator of the Wnt pathway, regulating adipogenesis, we wanted to investigate the role of sFRP5 variants in human, monogenic obesity by performing mutation analysis. We screened the complete sFRP5 coding region in 622 obese children and adolescents and 503 lean control individuals by high-resolution melting curve analysis and direct sequencing. We found a total of 15 sequence variants in sFRP5, 10 of which resulted in a non-synonymous amino acid change. Five of these variants were, to our knowledge, not previously reported. For one of the variants (c.-3G>A), we identified a trend towards association between the variant frequency and the obese phenotype. We argue that, when looking at conservation and location inside known protein domains, several of the identified variants (D103N, A113V, K212N and H317L), may affect sFRP5 protein function. In addition, we found c.-3G>A, residing in the Kozak sequence, with a lower frequency in cases compared to controls. However, functional studies investigating the effect of sFRP5 variants on protein function are necessary to determine the true role of sFRP5 genetic variation in human, monogenic obesity.
authors: Department of Medical Genetics, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium.
authors: Jasmijn K
authors: JK
authors: Van Camp
authors: Department of Medical Genetics, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium.
authors: Sigri
authors: S
authors: Beckers
authors: Department of Medical Genetics, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium.
authors: Doreen
authors: D
authors: Zegers
authors: Department of Paediatrics, Antwerp University Hospital, Wilrijkstraat 10, 2650, Antwerp, Belgium.
authors: Stijn L
authors: SL
authors: Verhulst
authors: Department of Paediatrics, Antwerp University Hospital, Wilrijkstraat 10, 2650, Antwerp, Belgium.
authors: Kim
authors: K
authors: Van Hoorenbeeck
authors: Department of Paediatrics, Jessa Hospital, Stadsomvaart 11, 3500, Hasselt, Belgium.
authors: Guy
authors: G
authors: Massa
authors: Department of Endocrinology, Diabetology and Metabolic Diseases, Antwerp University Hospital, Wilrijkstraat 10, 2650, Antwerp, Belgium.
authors: An
authors: A
authors: Verrijken
authors: Department of Paediatrics, Antwerp University Hospital, Wilrijkstraat 10, 2650, Antwerp, Belgium.
authors: Kristine N
authors: KN
authors: Desager
authors: Department of Endocrinology, Diabetology and Metabolic Diseases, Antwerp University Hospital, Wilrijkstraat 10, 2650, Antwerp, Belgium.
authors: Luc F
authors: LF
authors: Van Gaal
authors: Department of Medical Genetics, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium. wim.vanhul@uantwerpen.be.
authors: Wim
authors: W
authors: Van Hul
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000293:Adolescent; D002648:Child; D005136:Eye Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D009711:Nucleotides / Q000235:genetics; D009765:Obesity / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D005136:Eye Proteins; D008565:Membrane Proteins; D009711:Nucleotides; C118511:SFRP5 protein, human; D000094742:Secreted Frizzled-Related Proteins
keywords: Genetics; Mutation analysis; Obesity; Wnt; sFRP