title: Rapid biomimetic mineralization of collagen fibrils and combining with human umbilical cord mesenchymal stem cells for bone defects healing.
abstract: Collagen biomineralization is regulated by complicated interactions between the collagen matrix and non-collagenous extracellular proteins. Here, the use of sodium tripolyphosphate to simulate the templating functional motif of the C-terminal fragment of non-collagenous proteins is reported, and a low molecular weight polyacrylic acid served as a sequestration agent to stabilize amorphous calcium phosphate into nanoprecursors. Self-assembled collagen fibrils served as a fixed template for achieving rapid biomimetic mineralization in vitro. Results demonstrated that, during the mineralization process, intrafibrillar and extrafibrillar hydroxyapatite mineral with collagen fibrils formed and did so via bottom-up nanoparticle assembly based on the non-classical crystallization approach in the presence of these dual biomimetic functional analogues. In vitro human umbilical cord mesenchymal stem cell (hUCMSC) culture found that the mineralized scaffolds have a better cytocompatibility in terms of cell viability, adhesion, proliferation, and differentiation into osteoblasts. A rabbit femoral condyle defect model was established to confirm the ability of the n-HA/collagen scaffolds to facilitate bone regeneration and repair. The images of gross anatomy, MRI, CT and histomorphology taken 6 and 12weeks after surgery showed that the biomimetic mineralized collagen scaffolds with hUCMSCs can promote the healing speed of bone defects in vivo, and both of the scaffolds groups performing better than the bone defect control group. As new bone tissue formed, the scaffolds degraded and were gradually absorbed. All these results demonstrated that both of the scaffolds and cells have better histocompatibility.
authors: Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China.
authors: Bihua
authors: B
authors: Ye
authors: Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China.
authors: Xueshi
authors: X
authors: Luo
authors: Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China.
authors: Zhiwen
authors: Z
authors: Li
authors: Department of Anesthesiology, Huizhou Central People's Hospital, Huizhou 516001, China.
authors: Caiping
authors: C
authors: Zhuang
authors: Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China. Electronic address: tlihuali@jnu.edu.cn.
authors: Lihua
authors: L
authors: Li
authors: Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China.
authors: Lu
authors: L
authors: Lu
authors: Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China.
authors: Shan
authors: S
authors: Ding
authors: Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China.
authors: Jinhuan
authors: J
authors: Tian
authors: Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China. Electronic address: tcrz9@jnu.edu.cn.
authors: Changren
authors: C
authors: Zhou
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002113:Calcification, Physiologic; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D005269:Femur / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D011817:Rabbits; D054457:Tissue Scaffolds / Q000737:chemistry*; D014470:Umbilical Cord / Q000378:metabolism*
chemical_list: D003094:Collagen
keywords: Bone regeneration; Collagen; Human umbilical cord mesenchymal stem cells; Mineralization; Self-assembly