title: Identification and expression analysis of IκB and NF-κB genes from Cyclina sinensis.
abstract: With the increasing economic importance of Cyclina sinensis aquaculture, interest in its defense mechanisms against pathogenic infection has grown in recent years. Inhibitor of nuclear factor-kappaB (IκB) and nuclear factor-kappaB (NF-κB) are proteins with central roles in many important physiological and pathological processes, such as innate immune responses. In this study, we identified CsIκB and CsNF-κB genes from a C. sinensis transcriptome library. In healthy adult clams, CsIκB and CsNF-κB genes were widely expressed in various tissues and highly expressed in hemocytes. Further, the expression levels of these genes were significantly increased in hemocytes challenged by Vibrio anguillarum, Micrococcus luteus and poly I:C. Inhibition of CsMyD88 expression by RNAi technology significantly altered the mRNA expression patterns of CsIκB and CsNF-κB as measured using quantitative real-time PCR. These results collectively indicated that the NF-κB signaling pathway, including CsIκB and CsNF-κB genes, might be involved in early innate immune responses and may be regulated by a MyD88-dependent signaling pathway in C. sinensis.
authors: Tianjin Key Laboratory of Animal and Plant Resistance, School of Life Sciences, Tianjin Normal University, Tianjin, 300387, PR China.
authors: Shan
authors: S
authors: Gao
authors: Tianjin Key Laboratory of Animal and Plant Resistance, School of Life Sciences, Tianjin Normal University, Tianjin, 300387, PR China; Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, 300071, PR China.
authors: Yipeng
authors: Y
authors: Ren
authors: Tianjin Key Laboratory of Animal and Plant Resistance, School of Life Sciences, Tianjin Normal University, Tianjin, 300387, PR China.
authors: Haijing
authors: H
authors: Zhang
authors: Tianjin Key Laboratory of Animal and Plant Resistance, School of Life Sciences, Tianjin Normal University, Tianjin, 300387, PR China. Electronic address: baopingpan@126.com.
authors: Baoping
authors: B
authors: Pan
authors: Tianjin Key Laboratory of Animal and Plant Resistance, School of Life Sciences, Tianjin Normal University, Tianjin, 300387, PR China.
authors: Hong
authors: H
authors: Gao
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D007113:Immunity, Innate; D007422:Intestines / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D000072000:NF-KappaB Inhibitor alpha / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011897:Random Allocation; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; D000072000:NF-KappaB Inhibitor alpha
keywords: Cyclina sinensis; Inhibitor of nuclear factor-kappaB; Innate immune response; MyD88-dependent signal pathway; Nuclear factor-kappaB