title: Intrauterine administration of autologous peripheral blood mononuclear cells (PBMCs) activated by HCG improves the implantation and pregnancy rates in patients with repeated implantation failure: a prospective randomized study.
abstract: Intrauterine administration of autologous peripheral blood mononuclear cells (PBMCs) activated by HCG in vitro was reported to improve implantation rates in patients with repeated failure of IVF-ET (in vitro fertilization-embryo transfer). In this article, the value of intrauterine administration of PBMCs before embryo transfer and its optimal cell culture method will be investigated.
  
  
  
  Patients who had not experienced successful pregnancy despite three or more IVF-ET sessions were enrolled in this study (n=240, 240 cycles). PBMCs were obtained from patients themselves and were cultured with HCG for 24 hours. Twenty-four hours later, PBMCs were then administered to the intrauterine cavity of that patient from the study group (n=93, 93 cycles). The control group (n=105, 105 cycles) underwent ET without intrauterine administration.
  
  
  
  Clinical pregnancy rate, implantation rate, and miscarriage rate in the PBMC-treated group (46.24% and 23.66%, n=43 and 22, respectively) were significantly higher than those in the non-treated group (20.95% and 11.43%, P<.05; n=22 and 12, respectively).
  
  
  
  These findings indicate that intrauterine administration of autologous PBMC activated by HCG in vitro effectively improves embryo implantation in patients with three or more IVF failures.
authors: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.|Obstetrics and Gynecology, Tongji Medical College, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors: Nan
authors: N
authors: Yu
authors: Obstetrics and Gynecology, Tongji Medical College, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors: Bo
authors: B
authors: Zhang
authors: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.
authors: Mei
authors: M
authors: Xu
authors: Obstetrics and Gynecology, Tongji Medical College, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors: Sheng
authors: S
authors: Wang
authors: Obstetrics and Gynecology, Tongji Medical College, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors: Rong
authors: R
authors: Liu
authors: Obstetrics and Gynecology, Tongji Medical College, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors: Jianli
authors: J
authors: Wu
authors: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.
authors: Jing
authors: J
authors: Yang
authors: Obstetrics and Gynecology, Tongji Medical College, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors: Ling
authors: L
authors: Feng
mesh_terms: D000022:Abortion, Spontaneous / Q000453:epidemiology / Q000628:therapy*; D001804:Blood Transfusion, Autologous; D001805:Blood Transfusion, Intrauterine; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D017708:Leukocyte Transfusion; D011247:Pregnancy; D018873:Pregnancy Rate
keywords: IVF outcome; peripheral blood mononuclear cells; repeated implantation failure