title: The in silico identification and characterization of a bread wheat/Triticum militinae introgression line.
abstract: The capacity of the bread wheat (Triticum aestivum) genome to tolerate introgression from related genomes can be exploited for wheat improvement. A resistance to powdery mildew expressed by a derivative of the cross-bread wheat cv. Tähti × T. militinae (Tm) is known to be due to the incorporation of a Tm segment into the long arm of chromosome 4A. Here, a newly developed in silico method termed rearrangement identification and characterization (RICh) has been applied to characterize the introgression. A virtual gene order, assembled using the GenomeZipper approach, was obtained for the native copy of chromosome 4A; it incorporated 570 4A DArTseq markers to produce a zipper comprising 2132 loci. A comparison between the native and introgressed forms of the 4AL chromosome arm showed that the introgressed region is located at the distal part of the arm. The Tm segment, derived from chromosome 7G, harbours 131 homoeologs of the 357 genes present on the corresponding region of Chinese Spring 4AL. The estimated number of Tm genes transferred along with the disease resistance gene was 169. Characterizing the introgression's position, gene content and internal gene order should not only facilitate gene isolation, but may also be informative with respect to chromatin structure and behaviour studies.
authors: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
authors: Michael
authors: M
authors: Abrouk
authors: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
authors: Barbora
authors: B
authors: Balcárková
authors: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
authors: Hana
authors: H
authors: Šimková
authors: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
authors: Eva
authors: E
authors: Komínkova
authors: Munich Information Center for Protein Sequences/Institute of Bioinformatics and Systems Biology, Institute for Bioinformatics and Systems Biology, Helmholtz Center Munich, Neuherberg, Germany.|Division of Cell Biology, Department of Clinical and Experimental Medicine, Bioinformatics Infrastructure for Life Sciences, Linköping University, Linköping, Sweden.
authors: Mihaela M
authors: MM
authors: Martis
authors: Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia.
authors: Irena
authors: I
authors: Jakobson
authors: Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia.
authors: Ljudmilla
authors: L
authors: Timofejeva
authors: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
authors: Elodie
authors: E
authors: Rey
authors: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
authors: Jan
authors: J
authors: Vrána
authors: Diversity Arrays Technology Pty Ltd, Canberra, ACT, Australia.
authors: Andrzej
authors: A
authors: Kilian
authors: Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia.
authors: Kadri
authors: K
authors: Järve
authors: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
authors: Jaroslav
authors: J
authors: Doležel
authors: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
authors: Miroslav
authors: M
authors: Valárik
mesh_terms: D001203:Ascomycota / Q000472:pathogenicity; D001483:Base Sequence; D001939:Bread; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics / Q000378:metabolism; D003198:Computer Simulation; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: GenomeZipper; alien introgression; chromosome rearrangement; chromosome translocation; comparative analysis; linkage drag