title: Dock10 regulates CD23 expression and sustains B-cell lymphopoiesis in secondary lymphoid tissue.
abstract: Dock10, a guanine nucleotide exchange factor for the Rho GTPases Rac1 and Cdc42, affects cell morphology, membrane protrusive activity, and cell movement. Dock10 is prominently expressed in lymphoid tissue and upregulated by IL-4 in B cells. To investigate the physiological role of Dock10, WT mice and Dock10 KO mice were used. KO mice showed decreased numbers of B cells in spleen, both follicular B cells and marginal zone B cells, and in peripheral blood, but not in bone marrow. The antiapoptotic effect of IL-4 in vitro, the migratory response to CXCL13 or CCL21 in vitro, and the whole genome expression profile were intact in spleen B cells from KO mice. CD23, the low-affinity receptor for immunoglobulin E, was overexpressed on follicular B cells from KO mice, suggesting that Dock10 negatively regulates membrane CD23 expression. Negative regulation of CD23 expression by Dock10 could play a role in B cell maturation and function.
authors: Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain; Servicio de Inmunología, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors: Azahara-María
authors: AM
authors: García-Serna
authors: Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain; Servicio de Inmunología, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors: María-José
authors: MJ
authors: Alcaraz-García
authors: Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain; Servicio de Inmunología, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors: Natalia
authors: N
authors: Ruiz-Lafuente
authors: Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain; Servicio de Inmunología, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors: Silvia
authors: S
authors: Sebastián-Ruiz
authors: Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain; Grupo de Cirugía Experimental, Centro de Investigación Biomédica en Red de enfermedades hepáticas y digestivas (CIBERedh), Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors: Carlos-Manuel
authors: CM
authors: Martínez
authors: Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain; Servicio de Inmunología, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors: María-Rosa
authors: MR
authors: Moya-Quiles
authors: Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain; Servicio de Inmunología, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors: Alfredo
authors: A
authors: Minguela
authors: Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain; Servicio de Inmunología, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors: Ana-María
authors: AM
authors: García-Alonso
authors: Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain; Departamento de Bioquímica y Biología Molecular B e Inmunología, Universidad de Murcia, Murcia, Spain.
authors: Elena
authors: E
authors: Martín-Orozco
authors: Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca (IMIB-Arrixaca), Murcia, Spain; Servicio de Inmunología, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain. Electronic address: antonio.parrado@carm.es.
authors: Antonio
authors: A
authors: Parrado
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D054421:Chemokine CCL21 / Q000378:metabolism; D054382:Chemokine CXCL13 / Q000378:metabolism; D018858:Germinal Center / Q000276:immunology*; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008221:Lymphoid Tissue / Q000378:metabolism*; D038041:Lymphopoiesis; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017455:Receptors, IgE / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000378:metabolism
chemical_list: D054421:Chemokine CCL21; D054382:Chemokine CXCL13; C561785:DOCK10 protein, mouse; D020662:Guanine Nucleotide Exchange Factors; D017455:Receptors, IgE; D015847:Interleukin-4; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein
keywords: B cell; CD23; Cdc42 GTPase; Dock10; Fc epsilon receptor II (Fcer2); Guanine nucleotide exchange factor (GEF); Rac GTPase