title: β-Ketoacyl-acyl Carrier Protein Synthase I (KASI) Plays Crucial Roles in the Plant Growth and Fatty Acids Synthesis in Tobacco.
abstract: Fatty acids serve many functions in plants, but the effects of some key genes involved in fatty acids biosynthesis on plants growth and development are not well understood yet. To understand the functions of 3-ketoacyl-acyl-carrier protein synthase I (KASI) in tobacco, we isolated two KASI homologs, which we have designated NtKASI-1 and NtKASI-2. Expression analysis showed that these two KASI genes were transcribed constitutively in all tissues examined. Over-expression of NtKASI-1 in tobacco changed the fatty acid content in leaves, whereas over-expressed lines of NtKASI-2 exhibited distinct phenotypic features such as slightly variegated leaves and reduction of the fatty acid content in leaves, similar to the silencing plants of NtKASI-1 gene. Interestingly, the silencing of NtKASI-2 gene had no discernibly altered phenotypes compared to wild type. The double silencing plants of these two genes enhanced the phenotypic changes during vegetative and reproductive growth compared to wild type. These results uncovered that these two KASI genes had the partially functional redundancy, and that the KASI genes played a key role in regulating fatty acids synthesis and in mediating plant growth and development in tobacco.
authors: Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Xuefu Road 88, Kunming 650223, China. yangtianquan@xtbg.org.cn.|University of Chinese Academy of Science, Beijing 100049, China. yangtianquan@xtbg.org.cn.
authors: Tianquan
authors: T
authors: Yang
authors: College of Life Sciences, Anhui Science and Technology University, Fengyang 233100, China. ronghua.xu08@gmail.com.
authors: Ronghua
authors: R
authors: Xu
authors: Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Xuefu Road 88, Kunming 650223, China. jhchen2016@163.com.
authors: Jianghua
authors: J
authors: Chen
authors: Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132, Kunming 650201, China. liuaizhong@mail.kib.ac.cn.
authors: Aizhong
authors: A
authors: Liu
mesh_terms: D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / Q000502:physiology*; D000595:Amino Acid Sequence; D053898:Biosynthetic Pathways; D002736:Chloroplasts / Q000201:enzymology; D017124:Conserved Sequence; D005227:Fatty Acids / Q000096:biosynthesis*; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D007527:Isoenzymes / Q000502:physiology*; D050356:Lipid Metabolism; D009928:Organ Specificity; D010641:Phenotype; D010802:Phylogeny; D063245:Plant Development; D018515:Plant Leaves / Q000201:enzymology; D010940:Plant Proteins / Q000502:physiology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000201:enzymology; D014026:Nicotiana / Q000201:enzymology* / Q000254:growth & development
chemical_list: D005227:Fatty Acids; D007527:Isoenzymes; D010940:Plant Proteins; D012333:RNA, Messenger; C078182:beta-ketoacyl-acyl carrier protein synthase I; D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase
keywords: 3-ketoacyl-ACP synthase I; fatty acid synthesis; gene silence; over-expression; tobacco