title: Automated multi-plug filtration cleanup for liquid chromatographic-tandem mass spectrometric pesticide multi-residue analysis in representative crop commodities.
abstract: An automated multi-plug filtration cleanup (m-PFC) method on modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) extracts was developed. The automatic device was aimed to reduce labor-consuming manual operation workload in the cleanup steps. It could control the volume and the speed of pulling and pushing cycles accurately. In this work, m-PFC was based on multi-walled carbon nanotubes (MWCNTs) mixed with other sorbents and anhydrous magnesium sulfate (MgSO4) in a packed tip for analysis of pesticide multi-residues in crop commodities followed by liquid chromatography with tandem mass spectrometric (LC-MS/MS) detection. It was validated by analyzing 25 pesticides in six representative matrices spiked at two concentration levels of 10 and 100μg/kg. Salts, sorbents, m-PFC procedure, automated pulling and pushing volume, automated pulling speed, and pushing speed for each matrix were optimized. After optimization, two general automated m-PFC methods were introduced to relatively simple (apple, citrus fruit, peanut) and relatively complex (spinach, leek, green tea) matrices. Spike recoveries were within 83 and 108% and 1-14% RSD for most analytes in the tested matrices. Matrix-matched calibrations were performed with the coefficients of determination >0.997 between concentration levels of 10 and 1000μg/kg. The developed method was successfully applied to the determination of pesticide residues in market samples.
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
authors: Yuhong
authors: Y
authors: Qin
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
authors: Jingru
authors: J
authors: Zhang
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
authors: Yuan
authors: Y
authors: Zhang
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
authors: Fangbing
authors: F
authors: Li
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
authors: Yongtao
authors: Y
authors: Han
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
authors: Nan
authors: N
authors: Zou
authors: Tianjin Bonna-Agela Technologies, Tianjin 300462, China.
authors: Haowei
authors: H
authors: Xu
authors: Tianjin Bonna-Agela Technologies, Tianjin 300462, China.
authors: Meiyuan
authors: M
authors: Qian
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China. Electronic address: canpingp@cau.edu.cn.
authors: Canping
authors: C
authors: Pan
mesh_terms: D010367:Arachis / Q000737:chemistry; D001331:Automation; D002957:Citrus / Q000737:chemistry; D018556:Crops, Agricultural / Q000737:chemistry*; D005374:Filtration / Q000379:methods*; D027845:Malus / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013662:Tea / Q000737:chemistry; D014675:Vegetables / Q000737:chemistry
chemical_list: D037742:Nanotubes, Carbon; D010573:Pesticide Residues; D013662:Tea
keywords: Automated methods; MWCNTs; Pesticide multi-residue analysis; QuEChERS; m-PFC