title: A two-photon fluorescent probe for exogenous and endogenous superoxide anion imaging in vitro and in vivo.
abstract: Herein, we report a novel quinoline derivative-based two-photon fluorescent probe 6-(dimethylamino)quinoline-2-benzothiazoline (HQ), which is capable of tracking superoxide anion in organisms with specific "turn-on" fluorescence response based on extension of π-conjugations and moderate ICT process. The probe exhibited favorable photophysical properties, a broad linear range and high photostability. It can specifically detect superoxide anion with a significant fluorescence enhancement and great linearity from 0 to 500μM in PBS buffer. Furthermore, HQ shows low cytotoxicity and excellent photostability toward living cells and organisms, which was able to monitor endogenous superoxide anion fluxes in living cells and in vivo. For the first time, endogenous superoxide anion in lung inflammation was visualized successfully by using HQ through two-photon microscopy, and the probe HQ shows great potential for fast in-situ detecting of inflammatory response in live organisms.
authors: Key Laboratory of Biomedical Polymers of Ministry of Education, Institute for Advanced Studies (IAS), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China; Key Laboratory of Analytical Chemistry for Biology and Medicine of Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Run-Qing
authors: RQ
authors: Li
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine of Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Zhi-Qiang
authors: ZQ
authors: Mao
authors: Key Laboratory of Biomedical Polymers of Ministry of Education, Institute for Advanced Studies (IAS), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Lei
authors: L
authors: Rong
authors: Key Laboratory of Electrochemical Power Source of Hubei Province, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Nian
authors: N
authors: Wu
authors: Key Laboratory of Biomedical Polymers of Ministry of Education, Institute for Advanced Studies (IAS), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Qi
authors: Q
authors: Lei
authors: Key Laboratory of Biomedical Polymers of Ministry of Education, Institute for Advanced Studies (IAS), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Jing-Yi
authors: JY
authors: Zhu
authors: Key Laboratory of Electrochemical Power Source of Hubei Province, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Lin
authors: L
authors: Zhuang
authors: Key Laboratory of Biomedical Polymers of Ministry of Education, Institute for Advanced Studies (IAS), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China. Electronic address: xz-zhang@whu.edu.cn.
authors: Xian-Zheng
authors: XZ
authors: Zhang
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine of Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China. Electronic address: zhhliu@whu.edu.cn.
authors: Zhi-Hong
authors: ZH
authors: Liu
mesh_terms: D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D007249:Inflammation / Q000473:pathology; D008168:Lung / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008958:Models, Molecular; D061848:Optical Imaging / Q000379:methods*; D011804:Quinolines / Q000737:chemistry; D000067996:RAW 264.7 Cells; D013481:Superoxides / Q000032:analysis*; D015027:Zebrafish
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D011804:Quinolines; D013481:Superoxides; C058836:benzothiazoline
keywords: Bioimaging; Lung inflammation; Specific sensing; Superoxide anion; Two-photon