title: Fatty acid preference of mycelium-bound lipase from a locally isolated strain of Geotrichum candidum.
abstract: Mycelium-bound lipase (MBL) was prepared using a strain of Geotrichum candidum isolated from local soil. At the time of maximum lipase activity (54 h), the mycelia to which the lipase was bound were harvested by filtration and centrifugation. Dry MBL was prepared by lyophilizing the mycelia obtained. The yield of MBL was 3.66 g/l with a protein content of 44.11 mg/g. The lipase activity and specific lipase activity were 22.59 and 510 U/g protein, respectively. The moisture content of the MBL was 3.85%. The activity of free (extracellular) lipase in the culture supernatant (after removal of mycelia) was less than 0.2 U/ml. The MBL showed selectivity for oleic acid over palmitic acid during hydrolysis of palm olein, indicating that the lipase from G. candidum displayed high substrate selectivity for unsaturated fatty acid containing a cis-9 double bond, even in crude form. This unique specificity of MBL could be a direct, simple and inexpensive way in the fats and oil industry for the selective hydrolysis or transesterification of cis-9 fatty acid residues in natural triacylglycerols.
authors: Department of Food Science, Faculty of Food Science and Technology, University Putra Malaysia, 43400, Serdang, Selangor DE, Malaysia.
authors: Joo Ling
authors: JL
authors: Loo
authors: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
authors: Oi Mlng
authors: OM
authors: Lai
authors: Biotechnology Division, Malaysia Agricultural Research and Development Institute, P.O. Box 12301, 50774, Kuala Lumpur, Malaysia.
authors: Kamariah
authors: K
authors: Long
authors: Department of Food Science, Faculty of Food Science and Technology, University Putra Malaysia, 43400, Serdang, Selangor DE, Malaysia. hasanah@putra.upm.edu.my.
authors: Hasanah Mohd
authors: HM
authors: Ghazali
keywords: Geotrichum candidum; Lipolytic activity; Mycelium-bound lipase; Selective hydrolysis