title: Refining the Use of Linkage Disequilibrium as a Robust Signature of Selective Sweeps.
abstract: During a selective sweep, characteristic patterns of linkage disequilibrium can arise in the genomic region surrounding a selected locus. These have been used to infer past selective sweeps. However, the recombination rate is known to vary substantially along the genome for many species. We here investigate the effectiveness of current (Kelly's [Formula: see text] and [Formula: see text]) and novel statistics at inferring hard selective sweeps based on linkage disequilibrium distortions under different conditions, including a human-realistic demographic model and recombination rate variation. When the recombination rate is constant, Kelly's [Formula: see text] offers high power, but is outperformed by a novel statistic that we test, which we call [Formula: see text] We also find this statistic to be effective at detecting sweeps from standing variation. When recombination rate fluctuations are included, there is a considerable reduction in power for all linkage disequilibrium-based statistics. However, this can largely be reversed by appropriately controlling for expected linkage disequilibrium using a genetic map. To further test these different methods, we perform selection scans on well-characterized HapMap data, finding that all three statistics-[Formula: see text] Kelly's [Formula: see text] and [Formula: see text]-are able to replicate signals at regions previously identified as selection candidates based on population differentiation or the site frequency spectrum. While [Formula: see text] replicates most candidates when recombination map data are not available, the [Formula: see text] and [Formula: see text] statistics are more successful when recombination rate variation is controlled for. Given both this and their higher power in simulations of selective sweeps, these statistics are preferred when information on local recombination rate variation is available.
authors: Mathematical Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom Complexity Institute, Nanyang Technological University, Singapore 637723 guysjacobs@ntu.edu.sg.
authors: Guy S
authors: 0000-0002-4698-7758
authors: GS
authors: Jacobs
authors: Mathematical Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom.
authors: Tim J
authors: 0000-0002-9163-0061
authors: TJ
authors: Sluckin
authors: Department of Biological Anthropology, University of Cambridge, Cambridge CB2 1QH, United Kingdom.
authors: Toomas
authors: 0000-0002-6297-7808
authors: T
authors: Kivisild
mesh_terms: D005828:Genetics, Population / Q000706:statistics & numerical data; D023281:Genomics; D060148:HapMap Project; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium; D008962:Models, Theoretical; D011995:Recombination, Genetic; D012641:Selection, Genetic
keywords: genetic map; linkage disequilibrium; positive selection; recombination rate