title: A Practical Method for Preparation of Fecal Microbiota Transplantation.
abstract: Clostridium difficile is a challenging infection that can be difficult to treat with antibiotic therapy. This chapter outlines the processing material for fecal microbiota transplantation (FMT), also known as stool transplant. Fecal transplantations are effective in treating recurrent C. difficile infection (CDI). FMT uses a stool sample collected from a healthy, screened donor to restore healthy microbiota in the colon of a patient with CDI for symptom resolution. Here, we describe a rapid method for FMT preparation that uses inexpensive and disposable materials.
authors: Department of Medicine, Gastrointestinal Diseases Research Unit, Kingston General Hospital, Queen's University, Kingston, ON, Canada.
authors: Elizabeth
authors: E
authors: Perez
authors: Department of Pathology & Molecular Medicine, St. Joseph's Healthcare Hamilton, McMaster University, Hamilton, ON, Canada.
authors: Christine H
authors: CH
authors: Lee
authors: Department of Medicine, Gastrointestinal Diseases Research Unit, Kingston General Hospital, Queen's University, Kingston, ON, Canada. eop@queensu.ca.
authors: Elaine O
authors: EO
authors: Petrof
mesh_terms: D016360:Clostridioides difficile / Q000472:pathogenicity* / Q000502:physiology; D003106:Colon / Q000382:microbiology / Q000473:pathology; D004761:Enterocolitis, Pseudomembranous / Q000382:microbiology / Q000473:pathology / Q000628:therapy*; D000069467:Fecal Microbiota Transplantation / Q000295:instrumentation / Q000379:methods*; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome / Q000502:physiology; D006801:Humans; D018579:Patient Selection / Q000941:ethics; D014019:Tissue Donors / Q000941:ethics
keywords: C. difficile infection; FMT; Fecal microbial transplantation; Human biotherapy; Microbiota; Specimen preparation; Stool transplant