title: Identification, characterization of selenoprotein W and its mRNA expression patterns in response to somatostatin 14, cysteamine hydrochloride, 17β-estradiol and a binary mixture of 17β-estradiol and cysteamine hydrochloride in topmouth culter (Erythroculter ilishaeformis).
abstract: In this study, a selenoprotein W cDNA was cloned from topmouth culter (Erythroculter ilishaeformis), and it was designated as EISelW. The EISelW open reading frame was composed of 261 base pairs (bp), encoding 86-amino-acid protein. The 5' untranslated region (UTR) consisted of 104 bp, and the 3'-UTR was composed of 365 bp. A selenocysteine insertion sequence (SECIS) element was found in the 3'-UTR of EISelW mRNA. The SECIS element was classified as form II because of a small additional apical loop presented in SECIS element of EISelW mRNA. Bioinformatic approaches showed that the secondary structure of EISelW was a β1-α1-β2-β3-β4-α2 pattern from amino-terminal to carboxy-terminal. Real-time PCR analysis of EISelW mRNAs expression in 17 tissues showed that the EISelW mRNA was predominantly expressed in liver, ovary, pituitary, various regions of the brain, spinal cord and head kidney. Study of intraperitoneal injection showed that the levels of EISelW mRNA in brain, liver, ovary and spleen were regulated by somatostatin 14 (SS14), 17β-estradiol (E2), cysteamine hydrochloride (CSH) and a binary mixture of E2 and CSH, dependent on the dosage. These results suggest that E2, SS14 and CSH status may affect tissues of selenium metabolism by regulating the expression of SelW mRNA, as SelW plays a central role in selenium metabolism.
authors: Department of Basic Medical Science, Huzhou University, 759 Erhuan East Road, Huzhou, 313000, Zhejiang, People's Republic of China. 02196@zjhu.edu.cn.|National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition (Zhejiang), Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, 759 Erhuan East Road, Huzhou, 313000, Zhejiang, People's Republic of China. 02196@zjhu.edu.cn.
authors: Haiyan
authors: H
authors: Dong
authors: Department of Biology, Institute of Resources and Environment, Henan Polytechnic University, Jiaozuo, 454000, Henan, People's Republic of China.
authors: Wenbo
authors: W
authors: Chen
authors: Department of Basic Medical Science, Huzhou University, 759 Erhuan East Road, Huzhou, 313000, Zhejiang, People's Republic of China.
authors: Chao
authors: C
authors: Sun
authors: Department of Basic Medical Science, Huzhou University, 759 Erhuan East Road, Huzhou, 313000, Zhejiang, People's Republic of China.
authors: Jianwei
authors: J
authors: Sun
authors: Department of Basic Medical Science, Huzhou University, 759 Erhuan East Road, Huzhou, 313000, Zhejiang, People's Republic of China.
authors: Yanlin
authors: Y
authors: Wang
authors: Department of Basic Medical Science, Huzhou University, 759 Erhuan East Road, Huzhou, 313000, Zhejiang, People's Republic of China.
authors: Chao
authors: C
authors: Xie
authors: Department of Basic Medical Science, Huzhou University, 759 Erhuan East Road, Huzhou, 313000, Zhejiang, People's Republic of China.
authors: Qianwen
authors: Q
authors: Fu
authors: National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition (Zhejiang), Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, 759 Erhuan East Road, Huzhou, 313000, Zhejiang, People's Republic of China.
authors: Junjie
authors: J
authors: Zhu
authors: National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition (Zhejiang), Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, 759 Erhuan East Road, Huzhou, 313000, Zhejiang, People's Republic of China. yjy@zjhu.edu.cn.
authors: Jinyun
authors: J
authors: Ye
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D003543:Cysteamine / Q000494:pharmacology*; D018076:DNA, Complementary / Q000235:genetics; D004347:Drug Interactions; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000378:metabolism; D008297:Male; D010053:Ovary / Q000378:metabolism; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D051151:Selenoprotein W / Q000235:genetics*; D013004:Somatostatin / Q000494:pharmacology*; D013154:Spleen / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D051151:Selenoprotein W; D004958:Estradiol; D013004:Somatostatin; D003543:Cysteamine
keywords: 17β-Estradiol; Cysteamine hydrochloride; Injection; Selenoprotein W; Somatostatin 14