title: Development of feather keratin nanoparticles and investigation of their hemostatic efficacy.
abstract: Chicken feathers are considered as the major waste in poultry industry, which are mostly constituted of keratin proteins. Development of feather keratin for biomedical application is very attractive for chicken feather recycling. Human hair keratins have been demonstrated the significant hemostatic efficacy in the previous studies, but there are few reports of feather keratin for the hemostatic application. Here, the chicken feather keratin nanoparticle was developed for use as a hemostatic agent. Keratin was extracted from chicken feather in the present study, and a modified ultrasonic dispersion method was used to prepare keratin nanoparticles. The characterizations of feather keratin extracts and nanoparticles were investigated, including electrophoretic separation, amino acid composition, particle size, zeta potential, morphology, chemical structure and crystal form. Additionally, the hemostatic efficacy in vitro and in vivo of keratin nanoparticles were also studied. The results of hemostatic tests showed that the bleeding time and blood loss in tail amputation and liver scratch rat models can be significantly decreased after application of feather keratin nanoparticles, which demonstrated the potential application of feather keratin nanoparticles for hemostasis.
authors: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China.
authors: Ju
authors: J
authors: Wang
authors: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China. Electronic address: shilei_hao@cqu.edu.cn.
authors: Shilei
authors: S
authors: Hao
authors: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China.
authors: Tiantian
authors: T
authors: Luo
authors: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China.
authors: Qian
authors: Q
authors: Yang
authors: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China. Electronic address: wangbc2000@126.com.
authors: Bochu
authors: B
authors: Wang
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000494:pharmacology; D002645:Chickens; D004353:Drug Evaluation, Preclinical; D005241:Feathers / Q000737:chemistry*; D006490:Hemostatics / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007633:Keratins / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D030161:Avian Proteins; D006490:Hemostatics; D007633:Keratins
keywords: Feather keratin; Hemostasis; Hemostat; Nanoparticles