title: Intravital Correlative Microscopy: Imaging Life at the Nanoscale.
abstract: Studying key biological events within complex model systems relies on dynamic and functional imaging at optimum spatial and temporal resolutions. Intravital correlative light and electron microscopy (intravital CLEM) combines imaging living multicellular model systems with electron microscopy, and offers full ultrastructural details of dynamic or transient events in vivo. However, routine use of intravital CLEM is hindered by multiple technological challenges faced when targeting a micron-size object (e.g., single cells or organelles) in a complex living organism. Recently, various approaches have been developed to overcome these limitations. In this review we outline the current methods and present the power of intravital CLEM in different fields of research. Finally, we describe approaches that will make intravital CLEM a routine, quantitative method for high-resolution cell biology in vivo.
authors: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, 69117 Germany.
authors: Matthia A
authors: MA
authors: Karreman
authors: Microenvironmental Niche in Tumorigenesis and Targeted Therapy (MN3T), Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1109, 67200 Strasbourg, France; Université de Strasbourg, 67000 Strasbourg, France; LabEx Medalis, Université de Strasbourg, 67000 Strasbourg, France; Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, 67000 Strasbourg, France.
authors: Vincent
authors: V
authors: Hyenne
authors: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, 69117 Germany. Electronic address: schwab@embl.de.
authors: Yannick
authors: Y
authors: Schwab
authors: Microenvironmental Niche in Tumorigenesis and Targeted Therapy (MN3T), Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1109, 67200 Strasbourg, France; Université de Strasbourg, 67000 Strasbourg, France; LabEx Medalis, Université de Strasbourg, 67000 Strasbourg, France; Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, 67000 Strasbourg, France. Electronic address: jacky.goetz@inserm.fr.
authors: Jacky G
authors: JG
authors: Goetz
mesh_terms: D000818:Animals; D006801:Humans; D007091:Image Processing, Computer-Assisted; D000069416:Intravital Microscopy / Q000379:methods*; D008854:Microscopy, Electron; D036103:Nanotechnology / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis; D013194:Staining and Labeling
keywords: CLEM; animal models; electron microscopy; fluorescence microscopy; in vivo