title: Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture.
abstract: Silk protein sericins, in the recent years, find application in cosmetics and pharmaceuticals and as biomaterials. We investigate the potential of sericin, extracted from both mulberry Bombyx mori and different non-mulberry sources, namely, tropical tasar, Antheraea mylitta; muga, Antheraea assama; and eri, Samia ricini, as growth supplement in serum-free culture medium. Sericin supplemented media containing different concentrations of sericins from the different species are examined for attachment, growth, proliferation, and morphology of fibrosarcoma cells. The optimum sericin supplementation seems to vary with the source of sericins. The results indicate that all the sericins promote the growth of L929 cells in serum-free culture media; however, S. ricini sericin seems to promote better growth of cells amongst other non-mulberry sericins.
authors: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors: Neety
authors: N
authors: Sahu
authors: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors: Shilpa
authors: S
authors: Pal
authors: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors: Sunaina
authors: S
authors: Sapru
authors: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors: Joydip
authors: J
authors: Kundu
authors: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors: Sarmistha
authors: S
authors: Talukdar
authors: Central Muga Eri Research and Training Institute, Lahdoigarh, Jorhat, Assam 785700, India.
authors: N Ibotambi
authors: NI
authors: Singh
authors: The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China.
authors: Juming
authors: J
authors: Yao
authors: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors: Subhas C
authors: 0000-0002-7170-2291
authors: SC
authors: Kundu
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000737:chemistry* / Q000378:metabolism*; D005240:Feasibility Studies; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D051379:Mice; D008970:Molecular Weight; D031625:Morus; D047030:Sericins / Q000737:chemistry*; D047011:Silk / Q000737:chemistry
chemical_list: D017077:Culture Media, Conditioned; D047030:Sericins; D047011:Silk