title: Resting-state functional connectivity by independent component analysis-based markers corresponds to areas of initial seizure propagation established by prior modalities from the hypothalamus.
abstract: The aims of this study were to evaluate a clinically practical functional connectivity protocol designed to blindly identify the corresponding areas of initial seizure propagation and also to differentiate these areas from remote secondary areas affected by seizure. The patients in this cohort had intractable epilepsy caused by intrahypothalamic hamartoma, which is the location of the ictal focus. The ictal propagation pathway is homogeneous and established, thus creating the optimum situation for the proposed method validation study.
  
  
  
  Twelve patients with seizures from hypothalamic hamartoma and 6 normal control patients underwent resting state functional MRI, using independent component analysis to identify network differences in patients. This was followed by seed-based connectivity measures to determine the extent of functional connectivity derangement between hypothalamus and these areas. The areas with significant change in connectivity were compared with the results of prior studies' modalities used to evaluate seizure propagation.
  
  
  
  The left amygdala-parahippocampal gyrus area, cingulate gyrus, and occipito-temporal gyrus demonstrated the highest derangement in connectivity with the hypothalamus, p < 0.01, corresponding to the initial seizure propagation areas established by prior modalities. Areas of secondary ictal propagation were differentiated from these initial locations by first being identified as an abnormal neuronal signal source via independent component analysis, but did not show significant connectivity directly with the known ictal focus.
  
  
  
  Non-invasive connectivity measures correspond to areas of initial ictal propagation and differentiate such areas from secondary ictal propagation, which may aid in ictal focus surgical disconnection planning and support the use of this newer modality for adjunctive information in epilepsy surgery evaluation.
authors: Baylor College of Medicine, 3989, Pediatric Neurology, Houston, Texas, United States ; varinab@bcm.edu.
authors: Varina Louise
authors: VL
authors: Boerwinkle
authors: Baylor College of Medicine, Pediatrics, Houston, Texas, United States ; aawilfon@texaschildrenshospital.org.
authors: Angus A
authors: AA
authors: Wilfong
authors: Baylor College of Medicine, Neurosurgery, Houston, Texas, United States ; djcurry@bcm.edu.
authors: Daniel J
authors: DJ
authors: Curry
keywords: Epilepsy; Functional connectivity; Independent component analysis (ICA); Partial seizures; Resting-state functional connectivity magnetic resonance imaging (R-fMRI)