title: Pathogen-associated porin turns IL-10 competent B-1a cells toward proinflammatory cytokine response.
abstract: Shigellosis is a major problem in the developing countries causing mortality and morbidity particularly among the children. Shigella spp. harbours the epithelial cells of the human colon to infect the host and spread the disease. We analyzed the response of B-1a cells, the major component of the mucosal immune system to porin of Shigella dysenteriae type 1. We show that porin while proliferating B-1a cells, deplete Siglec-G, the inhibitory molecule present on B-1a cells. Adjuvanticity of porin has been shown to govern innate signaling for promoting host adaptive immune response. Up-regulation of CD69 and CD40 denotes activation of the cells parallel to abrogation of Siglec-G. As a result of cell activation, porin stimulated the inflammatory cytokines of CD5+B-1a cells, otherwise rich in IL-10. The work shows B-1a cell responses promote the immunopotentiating activity of porin.
authors: Division of Immunology, National Institute of Cholera and Enteric Diseases, Kolkata, India.
authors: Amlan Kanti
authors: AK
authors: Ghosh
authors: Division of Immunology, National Institute of Cholera and Enteric Diseases, Kolkata, India.
authors: Debolina
authors: D
authors: Sinha
authors: Division of Immunology, National Institute of Cholera and Enteric Diseases, Kolkata, India.
authors: Ratna
authors: R
authors: Biswas
authors: Division of Immunology, National Institute of Cholera and Enteric Diseases, Kolkata, India. Electronic address: shigellaporin@yahoo.com.
authors: Tapas
authors: T
authors: Biswas
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D003106:Colon / Q000473:pathology; D004405:Dysentery, Bacillary / Q000276:immunology*; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018272:Porins / Q000276:immunology*; D034741:RNA, Small Interfering / Q000235:genetics; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D012762:Shigella dysenteriae / Q000276:immunology*; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D018417:Th1 Cells / Q000276:immunology; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D018836:Inflammation Mediators; D037102:Lectins; D018272:Porins; D034741:RNA, Small Interfering; D011947:Receptors, Antigen, B-Cell; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; C522403:Siglecg protein, mouse; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; D016753:Interleukin-10
keywords: Activation molecules; B-1a cells; Cytokines; Porin; Siglec-G