title: Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems.
abstract: We present here the complete genomic sequence of a rifampin-resistant derivative of the Escherichia coli K-12 laboratory strain ER1821, engineered to be deficient in all known restriction systems, making it suitable for generating unbiased libraries from organisms with non-K-12 methylation patterns. The ER1821R genome is most closely related to that of DH1, another popular cloning strain (both derived from MM294), but is deleted for the e14 prophage (McrA(-)) and the immigration control (McrBC(-) EcoKI R(-) M(-) Mrr(-)) loci.
authors: University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA michael.jobling@ucdenver.edu.
authors: Michael G
authors: MG
authors: Jobling
authors: New England Biolabs, Ipswich, Massachusetts, USA.
authors: Elisabeth A
authors: EA
authors: Raleigh
authors: University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.
authors: Daniel N
authors: DN
authors: Frank