title: Ischemia-induced Angiogenesis is Attenuated in Aged Rats.
abstract: To study whether focal angiogenesis is induced in aged rodents after permanent distal middle cerebral artery occlusion (MCAO), young adult (3-month-old) and aged (24-month-old) Fisher 344 rats underwent MCAO and sacrificed up to two months after MCAO. Immunohistochemistry and synchrotron radiation microangiography were performed to examine the number of newly formed blood vessels in both young adult and aged rats post-ischemia. We found that the number of capillaries and small arteries in aged brain was the same as young adult brain. In addition, we found that after MCAO, the number of blood vessels in the peri-infarct region of ipsilateral hemisphere in aged ischemic rats was significantly increased compared to the aged sham rats (p<0.05). We also confirmed that ischemia-induced focal angiogenesis occurred in young adult rat brain while the blood vessel density in young adult ischemic brain was significantly higher than that in the aged ischemic brain (p<0.05). Our data suggests that focal angiogenesis in aged rat brain can be induced in response to ischemic brain injury, and that aging impedes brain repairing and remodeling after ischemic stroke, possible due to the limited response of angiogenesis.
authors: 1Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China; 2Department of Orthopaedic surgery, School of medicine, Stanford University, CA 94305, USA.
authors: Yaohui
authors: Y
authors: Tang
authors: 3Department of Neurology, the First Affiliated Hospital, Wenzhou Medical University, Zhejiang, 325000, China.
authors: Liuqing
authors: L
authors: Wang
authors: 5Shanghai Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200030, China.
authors: Jixian
authors: J
authors: Wang
authors: 1Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China.
authors: Xiaojie
authors: X
authors: Lin
authors: 1Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China.
authors: Yongting
authors: Y
authors: Wang
authors: 3Department of Neurology, the First Affiliated Hospital, Wenzhou Medical University, Zhejiang, 325000, China; 4Department of Pharmacology and Neuroscience, University of North Texas Health Science Center at Fort Worth, TX 76203, USA.
authors: Kunlin
authors: K
authors: Jin
authors: 1Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China; 5Shanghai Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200030, China.
authors: Guo-Yuan
authors: GY
authors: Yang
keywords: aging; angiogenesis; brain; focal cerebral ischemia; rat