title: Immunolocalization on Whole Anther Chromosome Spreads for Male Meiosis.
abstract: Immunolocalization of cells undergoing meiosis has proven to be one of the most important tools to decipher chromatin-associated protein dynamics and causal relationships. Here, we describe a protocol established for maize which is easily adaptable to other plants, for example, with minor modifications to Arabidopsis as stated here. In contrast to many other protocols, the following protocol is based on fixation by a 3:1 mixture of ethanol and acetic acid. Spreading of cells is followed by freeze-shattering, protein antigenicity retrieval by a hot citrate buffer bath, antibody incubations and washes, and DNA staining.
authors: Department of Horticultural Science, University of Minnesota, Alderman Hall, 1970 Folwell Ave., St. Paul, MN, USA.
authors: Stefanie
authors: S
authors: Dukowic-Schulze
authors: Department of Horticultural Science, University of Minnesota, Alderman Hall, 1970 Folwell Ave., St. Paul, MN, USA.
authors: Anthony
authors: A
authors: Harris
authors: Department of Horticultural Science, University of Minnesota, Alderman Hall, 1970 Folwell Ave., St. Paul, MN, USA. chenx481@umn.edu.
authors: Changbin
authors: C
authors: Chen
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D032461:Chromosomes, Plant; D035264:Flowers / Q000235:genetics*; D007124:Immunoenzyme Techniques / Q000379:methods*; D008540:Meiosis; D003313:Zea mays / Q000235:genetics*
keywords: Anthers; Arabidopsis; Chromosome spread; Immunolocalization; Maize; Meiosis; Plants