title: Characterization of a cadmium resistance Lactococcus lactis subsp. lactis strain by antioxidant assays and proteome profiles methods.
abstract: Heavy metal contamination poses a major threat to the environment and human health for their potential toxicity and non-biodegradable properties. At present, some probiotics bacteria are reported to have great potential to eliminate heavy metals from food and water. In this study, resistance properties of a newly isolated Lactococcus lactis subsp. lactis for cadmium were studied by antioxidant assays and proteomics analysis. Antioxidant capacity of this strain was significantly activated under cadmium stress indicated by Fenton reaction, DPPH assay, SOD assay and GSH assay. Intracellular antioxidant enzyme systems, such as superoxide dismutase, glutathione reductase and catalase were suggested to play vital roles in the activated antioxidant capacity. The up-regulated cadA was associated with the activated P-type ATPases that plays an important role in cadmium resistance. Proteomics analysis identified 12 over-expressed proteins under 50mg/L cadmium stress and these proteins are abundant in oxidative stress response and energy metabolism regulation, which were considered as consequences as cadmium resistance of the strain. Thus, the probiotics Lactococcus lactis subsp. lactis may resist cadmium stress through antioxidant approach and enhanced energy metabolism. The food grade lactis strain may be applied in metal decontamination in environment and food/feed.
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors: Yao
authors: Y
authors: Sheng
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors: Xuan
authors: X
authors: Yang
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors: Yuanyuan
authors: Y
authors: Lian
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors: Boyang
authors: B
authors: Zhang
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing 100083, China.
authors: Xiaoyun
authors: X
authors: He
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing 100083, China.
authors: Wentao
authors: W
authors: Xu
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing 100083, China. Electronic address: hkl009@163.com.
authors: Kunlun
authors: K
authors: Huang
mesh_terms: D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002104:Cadmium / Q000633:toxicity*; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D013294:Lactococcus lactis / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D019936:Probiotics / Q000494:pharmacology; D020543:Proteome / Q000235:genetics / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D020543:Proteome; D002104:Cadmium; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant capacity; Cadmium; Heavy metal; Lactococcus lactis subsp. lactis; Proteome profiles