title: Development of a HPLC-MS/MS method for the simultaneous determination of nifedipine and lidocaine in human plasma.
abstract: The method for simultaneous determination of nifedipine (NIF) and lidocaine (LID) in human plasma by one-step sample preparation has been developed for the first time. Due to the photosensitivity of nifedipine and its low plasma concentrations a precise and reliable method was required. The method involved liquid-liquid extraction (methyl tert-butyl ether, MTBE), and 10μL of the resulting sample was analyzed by HPLC-MS/MS. Chromatographic separation was achieved on an YMC-Triart C18 HPLC column (100×2.0mm; S-5μm 12nm). The mobile phase was methanol:water, 60:40 (v/v) and contained 0.15% acetic acid. The linearity of the method was established in the concentration ranges of 0.5-50ng/mL for NIF and 1.0-500ng/mL for LID. Photodestruction of NIF under ambient light was evaluated. The validated method was successfully applied to analyze human plasma samples after rectal application of the drug (1g) containing 2.0% LID and 0.3% NIF.
authors: Bioanalytical Laboratory CSU "Analytical Spectrometry", Russia; Bioanalytical Laboratory CSU "Analytical Spectrometry," Peter the Great St. Petersburg Polytechnic University, Box 27, Gzhatskaya str. 27, 198220, St. Petersburg, Russia. Electronic address: a.grigoriev@csuas.ru.
authors: Alexander
authors: A
authors: Grigoriev
authors: Bioanalytical Laboratory CSU "Analytical Spectrometry", Russia.
authors: Alexandra
authors: A
authors: Nikitina
authors: Bioanalytical Laboratory CSU "Analytical Spectrometry", Russia.
authors: Irina
authors: I
authors: Yaroshenko
authors: Bioanalytical Laboratory CSU "Analytical Spectrometry", Russia; Bioanalytical Laboratory CSU "Analytical Spectrometry," Peter the Great St. Petersburg Polytechnic University, Box 27, Gzhatskaya str. 27, 198220, St. Petersburg, Russia.
authors: Alla
authors: A
authors: Sidorova
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D006801:Humans; D008012:Lidocaine / Q000032:analysis / Q000097:blood*; D009543:Nifedipine / Q000032:analysis / Q000097:blood*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
chemical_list: D008012:Lidocaine; D009543:Nifedipine
keywords: HPLC–MS/MS; Lidocaine; Liquid–liquid extraction; Nifedipine; Pharmacokinetics; Photosensitivity