title: Cloning and expression analysis of innate immune genes from red sea bream to assess different susceptibility to megalocytivirus infection.
abstract: As suggested by the Office International des Epizooties (OIE), fishes belonging to the genus Oplegnathus are more sensitive to megalocytivirus infection than other fish species including red sea bream (Pagrus major). To assess the roles of the innate immune response to these different susceptibilities, we cloned the genes encoding inflammatory factors including IL-8 and COX-2, and the antiviral factor like Mx from red sea bream for the first time and performed phylogenetic and structural analysis. Analysed expression levels of IL-1β, IL-8 and COX-2 and the antiviral factor like Mx genes performed with in vivo challenge experiment showed no difference in inflammatory gene expression or respiratory burst activity between red sea bream and rock bream (Oplegnathus fasciatus). However, the Mx gene expression levels in red sea bream were markedly higher than those in rock bream, suggesting the importance of type I interferon (IFN)-induced proteins, particularly Mx, during megalocytivirus infection, rather than inflammation-related genes. The in vitro challenge experiments using embryonic primary cultures derived from both fish species showed no difference in cytopathic effects (CPE), viral replication profiles, and inflammatory and Mx gene expression pattern between the two fish species.
authors: Namhae Fisheries Hatchery Station, Korea Fisheries Resources Agency, Wando, South Korea.
authors: J W
authors: JW
authors: Jin
authors: Department of Aquatic Life Medicine, Pukyong National University, Busan, South Korea.
authors: Y C
authors: YC
authors: Kim
authors: Department of Marine Biotechnology, Gangneung -Wonju National University, Gangneung, South Korea.
authors: S
authors: S
authors: Hong
authors: Pathology Division, National Fisheries Research & Development Institute, Busan, South Korea.
authors: M S
authors: MS
authors: Kim
authors: Department of Aquatic Biomedical Science, Jeju National University, Jeju, South Korea.
authors: J B
authors: JB
authors: Jeong
authors: Department of Aquatic Life Medicine, Pukyong National University, Busan, South Korea.
authors: H D
authors: HD
authors: Jeong
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics*; D007497:Iridoviridae / Q000276:immunology*; D021541:Sea Bream; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D029941:Fish Proteins
keywords: innate immune genes; megalocytivirus; red sea bream; rock bream; susceptibility