title: Development of a Monoclonal Antibody Against Porcine Circovirus2 Cap Protein.
abstract: Female BALB/c mice were immunized with a commercial PCV2 vaccine, and a monoclonal antibody (MAb) designated as 3H11 was achieved by hybridoma techniques. The MAb specifically reacted with Cap protein of PCV2, which has been identified by western blot. Immunoperoxidase monolayer assay results showed that 3H11 did not cross-react with PCV1-infected cells. Therefore, this work suggested that 3H11 could be a useful tool as a specific diagnostic reagent for PCV2 research.
authors: 1 College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou, China .
authors: Yanli
authors: Y
authors: Gu
authors: 1 College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou, China .
authors: Dingding
authors: D
authors: Zheng
authors: 1 College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou, China .
authors: Yudan
authors: Y
authors: Jin
authors: 1 College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou, China .
authors: Liying
authors: L
authors: Hao
authors: 1 College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou, China .
authors: Yajie
authors: Y
authors: Xuan
authors: 1 College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou, China .
authors: Wenqiang
authors: W
authors: Pang
authors: 1 College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou, China .
authors: Yuxin
authors: Y
authors: Huang
authors: 1 College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou, China .
authors: Xiangdong
authors: X
authors: Li
authors: 1 College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou, China .
authors: Junhua
authors: J
authors: Deng
authors: 1 College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou, China .|2 National Research Center for Veterinary Medicine , Luoyang, China .
authors: Kegong
authors: K
authors: Tian
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology*; D017925:Circovirus / Q000276:immunology* / Q000472:pathogenicity; D018604:Epitope Mapping; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins