title: The flexible feedstock concept in Industrial Biotechnology: Metabolic engineering of Escherichia coli, Corynebacterium glutamicum, Pseudomonas, Bacillus and yeast strains for access to alternative carbon sources.
abstract: Most biotechnological processes are based on glucose that is either present in molasses or generated from starch by enzymatic hydrolysis. At the very high, million-ton scale production volumes, for instance for fermentative production of the biofuel ethanol or of commodity chemicals such as organic acids and amino acids, competing uses of carbon sources e.g. in human and animal nutrition have to be taken into account. Thus, the biotechnological production hosts E. coli, C. glutamicum, pseudomonads, bacilli and Baker's yeast used in these large scale processes have been engineered for efficient utilization of alternative carbon sources. This flexible feedstock concept is central to the use of non-glucose second and third generation feedstocks in the emerging bioeconomy. The metabolic engineering efforts to broaden the substrate scope of E. coli, C. glutamicum, pseudomonads, B. subtilis and yeasts to include non-native carbon sources will be reviewed. Strategies to enable simultaneous consumption of mixtures of native and non-native carbon sources present in biomass hydrolysates will be summarized and a perspective on how to further increase feedstock flexibility for the realization of biorefinery processes will be given.
authors: Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld University, Bielefeld, Germany. Electronic address: volker.wendisch@uni-bielefeld.de.
authors: Volker F
authors: VF
authors: Wendisch
authors: Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld University, Bielefeld, Germany.
authors: Luciana Fernandes
authors: LF
authors: Brito
authors: Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld University, Bielefeld, Germany.
authors: Marina
authors: M
authors: Gil Lopez
authors: Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld University, Bielefeld, Germany.
authors: Guido
authors: G
authors: Hennig
authors: Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld University, Bielefeld, Germany.
authors: Johannes
authors: J
authors: Pfeifenschneider
authors: Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld University, Bielefeld, Germany.
authors: Elvira
authors: E
authors: Sgobba
authors: Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld University, Bielefeld, Germany.
authors: Kareen H
authors: KH
authors: Veldmann
mesh_terms: D001407:Bacillus / Q000235:genetics / Q000378:metabolism*; D056804:Biofuels / Q000382:microbiology; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000737:chemistry* / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D060847:Metabolic Engineering; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism*
chemical_list: D056804:Biofuels; D002244:Carbon
keywords: Bacillus; Bio-based value-added chemicals; Biofuels; Biorefinery; C. glutamicum; CO(2); E. coli; Flexible feedstock concept; Hydrolysates; Lignocellulosics; Metabolic engineering; Methanol; Pseudomonas; Second generation feedstocks; Strain development; Yeast