title: Listeria monocytogenes induces mast cell extracellular traps.
abstract: Mast cells play an essential role in different immunological phenomena including allergy and infectious diseases. Several bacteria induce mast cell activation leading to degranulation and the production of several cytokines and chemokines. However, mast cells also have different microbicidal activities such as phagocytosis and the release of DNA with embedded granular proteins known as Mast Cell Extracellular Traps (MCETs). Although previous reports indicate that extracellular bacteria are able to induce MCETs little is known if intracellular bacteria can induce these structures. In this work, we evaluated MCETs induction by the intracellular bacteria Listeria monocytogenes. We found that mast cells released DNA after stimulation with L. monocytogenes, and this DNA was complexed to histone and tryptase. Before extracellular DNA release, L. monocytogenes induced modifications to the mast cell nuclear envelope and DNA was detected outside the nucleus. L. monocytogenes stimulated mast cells to produce significant amounts of reactive oxygen species (ROS) and blocking NADPH oxidase diminished DNA release by mast cells. Finally, MCETs showed antimicrobial activity against L. monocytogenes that was partially blocked when β-hexosaminidase activity was inhibited. These results show that L. monocytogenes induces mast cells to produce microbicidal MCETs, suggesting a role for mast cells in containing infection beyond the induction of inflammation.
authors: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ENCB-IPN, Mexico.
authors: Marcia
authors: M
authors: Campillo-Navarro
authors: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ENCB-IPN, Mexico.
authors: Kahiry
authors: K
authors: Leyva-Paredes
authors: Department of Cell Biology, Cinvestav, Instituto Politécnico Nacional, Mexico.
authors: Luis
authors: L
authors: Donis-Maturano
authors: Department of Cell Biology, Instituto Nacional de Perinatología, SS, Mexico.
authors: Marco
authors: M
authors: González-Jiménez
authors: Department of Cell Biology, Instituto Nacional de Perinatología, SS, Mexico.
authors: Yuriria
authors: Y
authors: Paredes-Vivas
authors: Woman Hospital, Research Department, SS, Mexico.
authors: Arturo
authors: A
authors: Cerbulo-Vázquez
authors: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ENCB-IPN, Mexico.
authors: Jeanet
authors: J
authors: Serafín-López
authors: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ENCB-IPN, Mexico.
authors: Blanca
authors: B
authors: García-Pérez
authors: Department of Immunology and The Center for Cancer Immunology Research, The University of Texas, MD Anderson Cancer Center, USA; The University of Texas Graduate School of Biological Sciences at Houston, TX, USA.
authors: Stephen E
authors: SE
authors: Ullrich
authors: Department of Cell Biology, Cinvestav, Instituto Politécnico Nacional, Mexico.
authors: Leopoldo
authors: L
authors: Flores-Romo
authors: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ENCB-IPN, Mexico; Unidad de Desarrollo e Investigación en Bioprocesos (UDIBI), Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ENCB-IPN, Mexico.
authors: Sonia M
authors: SM
authors: Pérez-Tapia
authors: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ENCB-IPN, Mexico.
authors: Sergio
authors: S
authors: Estrada-Parra
authors: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ENCB-IPN, Mexico.
authors: Iris
authors: I
authors: Estrada-García
authors: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ENCB-IPN, Mexico; Unidad de Desarrollo e Investigación en Bioprocesos (UDIBI), Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ENCB-IPN, Mexico. Electronic address: rommelchacons@yahoo.com.mx.
authors: Rommel
authors: R
authors: Chacón-Salinas
mesh_terms: D002460:Cell Line; D004247:DNA / Q000378:metabolism; D065206:Extracellular Traps / Q000276:immunology* / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000276:immunology*; D006801:Humans; D008089:Listeria monocytogenes / Q000276:immunology*; D008088:Listeriosis; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism* / Q000648:ultrastructure; D009685:Nuclear Envelope / Q000648:ultrastructure; D010587:Phagocytosis / Q000276:immunology; D017382:Reactive Oxygen Species / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D006657:Histones; D017382:Reactive Oxygen Species; D004247:DNA; D001619:beta-N-Acetylhexosaminidases
keywords: Listeria monocytogenes; Mast cell; Mast cell extracellular trap; β-Hexosaminidase