title: Collagen/chitosan porous bone tissue engineering composite scaffold incorporated with Ginseng compound K.
abstract: In this study, suitable scaffold materials for bone tissue engineering were successfully prepared using fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate. Porous composite scaffolds were prepared by freeze drying method. The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold. The scaffold was characterized for pore size, swelling, density, degradation, mineralization, cell viability and attachment, and its morphological features were examined using scanning electron microscopy. This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
authors: Department of Physiology, College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan-city, Jeollabuk-Do 54596, Republic of Korea. Electronic address: auromuthu@gmail.com.
authors: Thangavelu
authors: T
authors: Muthukumar
authors: Department of Physiology, College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan-city, Jeollabuk-Do 54596, Republic of Korea.
authors: Adithan
authors: A
authors: Aravinthan
authors: Department of Physiology, College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan-city, Jeollabuk-Do 54596, Republic of Korea.
authors: Judith
authors: J
authors: Sharmila
authors: Department of Physiology, College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan-city, Jeollabuk-Do 54596, Republic of Korea.
authors: Nam Soo
authors: NS
authors: Kim
authors: Department of Physiology, College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan-city, Jeollabuk-Do 54596, Republic of Korea. Electronic address: jhkim1@jbnu.ac.kr.
authors: Jong-Hoon
authors: JH
authors: Kim
mesh_terms: D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D036145:Ginsenosides / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D013977:Tibia / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D048271:Chitosan; D003094:Collagen; D036145:Ginsenosides; C112772:ginsenoside compound K
keywords: Bone tissue engineering; Chitosan; Collagen; Freeze drying; Ginseng Compound K; Hydroxyapatite