title: Lack of Effects of Recombinant Human Bone Morphogenetic Protein2 on Angiogenesis in Oral Squamous Cell Carcinoma Induced in the Syrian hamster Cheek Pouch.
abstract: Recombinant human bone morphogenetic protein2 (rhBMP2 ), a member of the TGF? family, has been used widely in recent years to regenerate defects of the maxillary and mandible bones. Such defects are sometimes caused by resection of oral squamous cell carcinoma (OSCC) yet the biologic effects of rhBMP2 on these carcinomas are not fully clear. The objective of this study was to determine histologically whether rhBMP2 produces adverse effects on angiogenesis during induction of OSCC, a biologic process critical for tumor formation in an experimental model in the buccal pouch of golden Syrian hamsters. Buccal cavities were exposed to painting with 0.5% DMBA in liquid paraffin three times a week for 14 weeks, then biopsies were taken. Division was into 2 groups: a study group of 10 hamsters receiving 0.25?g/ml of rhBMP2 in the 3rd and 6th weeks; and a control group of 10 hamsters which did not receive any additional treatment. VEGF expression and microvessel density were measured but no differences were noted between the two groups. According to this study, rhBMP2 does not stimulate angiogenesis during induction of OCSSs.
authors: Department of Oral histology and Pathology, Faculty of Dentistry, University of Damascus, Damascus, Syria Email : dr.khaled.zaid@gmail.com.
authors: Khaled Waleed
authors: KW
authors: Zaid
authors: Bander Mossa
authors: BM
authors: Nhar
authors: Salman Mohammed
authors: SM
authors: Ghadeer Alanazi
authors: Rashad
authors: R
authors: Murad
authors: Ahmad
authors: A
authors: Domani
authors: Awadh Jamman
authors: AJ
authors: Alhafi
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000378:metabolism*; D002610:Cheek; D006224:Cricetinae; D006801:Humans; D008297:Male; D008647:Mesocricetus; D055806:Microvessels / Q000378:metabolism; D009024:Morphogenesis / Q000502:physiology; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D015127:9,10-Dimethyl-1,2-benzanthracene