title: MicroRNA-2400 promotes bovine preadipocyte proliferation.
abstract: MicroRNAs (miRNAs) play critical roles in the proliferation of bovine preadipocytes. miR-2400 is a novel and unique miRNA from bovines. In the present study, we separated and identified preadipocytes from bovine samples. miR-2400 overexpression increased the rate of preadipocyte proliferation, which was analyzed with a combination of EdU and flow cytometry. Simultaneously, functional genes related to proliferation (PCNA, CCND2, CCNB1) were also increased, which was detected by real-time PCR. Furthermore, luciferase reporter assays showed that miR-2400 bound directly to the 3'untranslated regions (3'UTRs) of PRDM11 mRNA. These data suggested that miR-2400 could promote preadipocyte proliferation by targeting PRDM11.
authors: The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors: Yao
authors: Y
authors: Wei
authors: The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors: Ya Feng
authors: YF
authors: Cui
authors: The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors: Hui Li
authors: HL
authors: Tong
authors: College of Life Sciences and Agriculture & Forestry, Qiqihar University, Qiqihar, Heilongjiang 161006, China.
authors: Wei Wei
authors: WW
authors: Zhang
authors: The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic address: yanyunqin@sohu.com.
authors: Yun Qin
authors: YQ
authors: Yan
mesh_terms: D020413:3' Untranslated Regions; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D002469:Cell Separation; D015536:Down-Regulation / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D002352:Carrier Proteins; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Bovine; PRDM11; Preadipocytes; Proliferation; miR-2400