title: XB130 is overexpressed in prostate cancer and involved in cell growth and invasion.
abstract: XB130 is a cytosolic adaptor protein involved in various physiological processes and oncogenesis of certain malignancies, but its role in the development of prostate cancer remains unclear. In current study, we examined XB130 expression in prostate cancer tissues and found that XB130 expression was remarkably increased in prostate cancer tissues and significantly correlated with increased prostate specific antigen (PSA), free PSA (f-PSA), prostatic acid phosphatase (PAP) and T classification. Patients with highly expressed XB130 had significantly decreased survival, which suggested XB130 as a possible prognostic indicator for prostate cancer. In vitro experiments showed that reduced XB130 expression restrained tumor growth both in vitro and in vivo. Furthermore, XB130 knockdown hindered transition of G1 to S phase in prostate cancer cell line DU145 and LNCap, which might contribute to the inhibition of cellular proliferation. Results from transwell assay demonstrated that downregulation of XB130 may attenuate invasion and metastasis of prostate cancer. Semiquantitative analysis of Western blot suggested that decreased XB130 expression was accompanied by diminished Akt signaling and EMT process. Thus, above observations suggest that XB130 may be a novel molecular marker and potent therapeutic target for prostate cancer.
authors: Department of Science and Training, General Hospital of Guangzhou Military Command of People's Liberation Army, Guangzhou, Guangdong, China.|Guangzhou Huabo Biopharmaceutical Research Institute, Guangzhou, Guangdong, China.
authors: Bin
authors: B
authors: Chen
authors: Department Of Pathology, Peking University Shenzhen Hospital, Shenzhen, China.
authors: Mengying
authors: M
authors: Liao
authors: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
authors: Qiang
authors: Q
authors: Wei
authors: Cancer Center, Traditional Chinese Medicine-Integrated Hospital of Southern Medical University, Guangzhou, Guangdong, China.
authors: Feiye
authors: F
authors: Liu
authors: Department of Urology, General Hospital of Guangzhou Military Command of People's Liberation Army, Guangzhou, Guangdong, China.
authors: Qinsong
authors: Q
authors: Zeng
authors: Department of Urology, General Hospital of Guangzhou Military Command of People's Liberation Army, Guangzhou, Guangdong, China.
authors: Wei
authors: W
authors: Wang
authors: Department of Urology, General Hospital of Guangzhou Military Command of People's Liberation Army, Guangzhou, Guangdong, China.
authors: Jun
authors: J
authors: Liu
authors: Sun Yat-Sen University, Guangzhou, China.
authors: Jianing
authors: J
authors: Hou
authors: Guangdong Provincial Key Laboratory of Geriatric Infection and Organ Function Support and Guangzhou Key Laboratory of Geriatric Infection and Organ Function Support, Guangzhou, Guangdong, China.|Center for Geriatrics, General Hospital of Guangzhou Military Command of People's Liberation Army, Guangzhou, Guangdong, China.
authors: Xinpei
authors: X
authors: Yu
authors: Center for Geriatrics, General Hospital of Guangzhou Military Command of People's Liberation Army, Guangzhou, Guangdong, China.
authors: Jian
authors: J
authors: Liu
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D001706:Biopsy; D063646:Carcinogenesis; D048708:Cell Growth Processes; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis; D051058:Oncogene Protein v-akt / Q000378:metabolism; D017430:Prostate-Specific Antigen / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: C521126:AFAP1L2 protein, human; D048868:Adaptor Proteins, Signal Transducing; D034741:RNA, Small Interfering; D051058:Oncogene Protein v-akt; D000135:Acid Phosphatase; C428439:prostatic acid phosphatase; D017430:Prostate-Specific Antigen
keywords: Akt; XB130; adaptor protein; invasion; proliferation