title: Physical association between a novel plasma-membrane structure and centrosome orients cell division.
abstract: In the last mitotic division of the epidermal lineage in the ascidian embryo, the cells divide stereotypically along the anterior-posterior axis. During interphase, we found that a unique membrane structure invaginates from the posterior to the centre of the cell, in a microtubule-dependent manner. The invagination projects toward centrioles on the apical side of the nucleus and associates with one of them. Further, a cilium forms on the posterior side of the cell and its basal body remains associated with the invagination. A laser ablation experiment suggests that the invagination is under tensile force and promotes the posterior positioning of the centrosome. Finally, we showed that the orientation of the invaginations is coupled with the polarized dynamics of centrosome movements and the orientation of cell division. Based on these findings, we propose a model whereby this novel membrane structure orchestrates centrosome positioning and thus the orientation of cell division axis.
authors: Division of Morphogenesis, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Japan.|Laboratoire de Biologie du Développement de Villefranche-sur-mer UMR7009, Observatoire Océanologique, Sorbonne Universités, UPMC Université Paris 06, CNRS, Villefranche-sur-Mer, France.
authors: Takefumi
authors: 0000-0002-0306-9578
authors: T
authors: Negishi
authors: National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Japan.
authors: Naoyuki
authors: N
authors: Miyazaki
authors: National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Japan.
authors: Kazuyoshi
authors: 0000-0001-9446-3652
authors: K
authors: Murata
authors: Laboratoire de Biologie du Développement de Villefranche-sur-mer UMR7009, Observatoire Océanologique, Sorbonne Universités, UPMC Université Paris 06, CNRS, Villefranche-sur-Mer, France.
authors: Hitoyoshi
authors: H
authors: Yasuo
authors: Division of Morphogenesis, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Japan.|Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies, Okazaki, Japan.
authors: Naoto
authors: N
authors: Ueno
mesh_terms: D000818:Animals; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism*; D018385:Centrosome / Q000378:metabolism*; D004817:Epidermis / Q000196:embryology; D008954:Models, Biological; D014561:Urochordata / Q000196:embryology
keywords: C. intestinalis; Ciliary positioning; cell biology; developmental biology; spindle orientation; stem cells