title: Heat-killed Lactobacillus Reuteri GMNL-263 Prevents Epididymal Fat Accumulation and Cardiac Injury in High-Calorie Diet-Fed Rats.
abstract: High-calorie diet-induced obesity leads to cardiomyocyte dysfunction and apoptosis. Impaired regulation of epididymal fat content in obese patients has been known to increase the risk of cardiac injury. In our study, a lactic acid bacteria, Lactobacillus reuteri GMNL-263, was evaluated for its potential to reduce body weight and body fat ratio and to prevent heart injury in rats with high-fat diet-induced obesity. Lactic acid bacteria supplementation restored the cardiac function and decreased the physiological changes in the heart of the obese rats. In addition, the Fas/Fas-associated protein pathway-induced caspase 3/e Poly polymerase mediated apoptosis in the cardiomyocytes of the obese rats was reversed in the Lr263-treated rats. These results reveal that fed with Lr-263 reduces body fat ratio, inhibits caspase 3-mediated apoptosis and restores cardiac function in obese rats through recovery of ejection fraction and fractional shortening. Our results indicated that the administration of Lr263 lactic acid bacteria can significantly down-regulate body fat and prevent cardiomyocyte injury in obese rats.
authors: 1. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.
authors: Po-Hsiang
authors: PH
authors: Liao
authors: 2. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.
authors: Wei-Wen
authors: WW
authors: Kuo
authors: 3. School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan.
authors: Dennis Jine-Yuan
authors: DJ
authors: Hsieh
authors: 4. Department of pathology, Changhua Christian Hospital, Changhua, Taiwan; 5. Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, Taiwan.
authors: Yu-Lan
authors: YL
authors: Yeh
authors: 6. Department of Nursing, Mei Ho University, Pingguang Road, Pingtung, Taiwan.
authors: Cecilia-Hsuan
authors: CH
authors: Day
authors: 7. Department of Research and Development, GenMont Biotech Incorporation, Tainan, Taiwan.
authors: Ya-Hui
authors: YH
authors: Chen
authors: 8. Department of Health, Tsao-Tun Psychiatric Center, Executive Yuan, Nantou, Taiwan.
authors: Sheng-Huang
authors: SH
authors: Chang
authors: 9. Department of Biotechnology, Bharathiar University, Coimbatore, India.
authors: V Vijaya
authors: VV
authors: Padma
authors: 7. Department of Research and Development, GenMont Biotech Incorporation, Tainan, Taiwan; 10. Institute of Biomedical Science, National Chung Hsing University, Taichung, Taiwan.
authors: Yi-Hsing
authors: YH
authors: Chen
authors: 1. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan; 11. Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan; 12. Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
authors: Chih-Yang
authors: CY
authors: Huang
mesh_terms: D000273:Adipose Tissue / Q000254:growth & development / Q000382:microbiology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D059305:Diet, High-Fat; D019587:Dietary Supplements; D004822:Epididymis / Q000187:drug effects / Q000254:growth & development / Q000382:microbiology / Q000503:physiopathology*; D006358:Hot Temperature; D052200:Limosilactobacillus reuteri / Q000378:metabolism*; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D009765:Obesity / Q000378:metabolism / Q000382:microbiology / Q000628:therapy*; D019936:Probiotics / Q000008:administration & dosage*; D051381:Rats
keywords: Cardiac dysfunction; Functional food; High-calorie diet; Obesity