title: Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features.
abstract: In South America, visceral leishmaniasis is a zoonotic disease with severe evolution characteristics in humans, and dogs are its main reservoir. In this context, this study aimed to evaluate the clinical status of dogs from a Brazilian endemic area naturally, at Barra Mansa municipality, infected with Leishmania infantum, in conjunction with their histopathological profile and, in order to determine possible markers of susceptibility or resistance to the disease, parasitic DNA load, cytokine and iNOS mRNA expression profiles were investigated in lymph nodes. High levels of IFN-ɣ and IL-6 mRNA were detected. Both IFN-ɣ and IL-6 mRNA were associated with disorganization of the corticomedullary region. IFN-ɣ and TNF-α mRNA were associated with the absence of follicular hyperplasia. The regulatory pathway was remarkable with IL-10 mRNA detection and its significant association with the severity of the disease. Plasmacytosis and sinus histiocytosis were associated with high loads of parasitic DNA, but there was no significant association between the parasite DNA load and animal clinical alterations. Since high parasitic loads were found in animals with or without symptoms, clinical examination cannot be considered as a criterion for disease susceptibility assessment.
authors: Pós-graduação em Medicina Veterinária, Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Santa Rosa, Niterói, RJ CEP 24230-340, Brazil. Electronic address: tassia.vasconcelos@gmail.com.
authors: Tassia Cristina Bello
authors: TC
authors: de Vasconcelos
authors: Unit Cytokines and Inflammation, Department of Infection & Epidemiology, 28 Rue du Docteur Roux, 75015 Paris, France.
authors: Noelle
authors: N
authors: Doyen
authors: Unit Cytokines and Inflammation, Department of Infection & Epidemiology, 28 Rue du Docteur Roux, 75015 Paris, France.
authors: Jean-Marc
authors: JM
authors: Cavaillon
authors: Pós-graduação em Medicina Veterinária, Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Santa Rosa, Niterói, RJ CEP 24230-340, Brazil.
authors: Sávio Freire
authors: SF
authors: Bruno
authors: Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ CEP 21040-360, Brazil.
authors: Monique Paiva
authors: MP
authors: de Campos
authors: Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ CEP 21040-360, Brazil.
authors: Luisa Helena Monteiro
authors: LH
authors: de Miranda
authors: Laboratório de Vigilância em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ CEP 21040-360, Brazil.
authors: Maria de Fátima
authors: Mde F
authors: Madeira
authors: Universidade Federal de São João del-Rei, campus Centro Oeste Dona Lindu, Rua Sebastião Gonçalves Coelho, 400, Bairro Chanadour, Divinópolis, MG CEP 35501-296, Brazil.
authors: Vinícius Silva
authors: VS
authors: Belo
authors: Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ CEP 21040-360, Brazil.
authors: Fabiano Borges
authors: FB
authors: Figueiredo
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D016054:DNA, Protozoan / Q000235:genetics; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D018314:Leishmania infantum / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D008198:Lymph Nodes / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*
chemical_list: D016207:Cytokines; D016054:DNA, Protozoan; D052247:Nitric Oxide Synthase Type II
keywords: Canine infection; Immunopathology; Visceral leishmaniasis