title: Equine antibody response to larval Parascaris equorum excretory-secretory products.
abstract: Parascaris equorum is an intestinal nematode of foals and young horses that can produce mild to severe pathology. Current diagnosis is limited to detection of patent infections, when parasite eggs are identified during fecal examinations. This study examined the use of larval P. equorum excretory-secretory (ES) products in a western blot test for diagnosis of prepatent equine P. equorum infection. Sera from adult mares negative for patent P. equorum infections, foals prior to consuming colostrum, and P. equorum infected foals were used as controls in this study. Study samples included sera from 18 broodmares prior to parturition and sera from their foals throughout the process of natural infection. Sera from study horses were examined for IgG(T) antibody recognition of ES products. Foals naturally infected with P. equorum possessed IgG(T) antibodies against 19kDa, 22kDa, 26kDa, and 34kDa ES products. However, passive transfer of colostral antibodies from mares was shown to preclude the use of the crude larval ES product-based western blot test for diagnosis of prepatent P. equorum infections in foals.
authors: W.P. Garrigus Building, Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546-0215, United States. Electronic address: sburk@otterbein.edu.
authors: Steffanie V
authors: SV
authors: Burk
authors: 4467 TAMU, Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843-4467, United States.
authors: Sriveny
authors: S
authors: Dangoudoubiyam
authors: W.P. Garrigus Building, Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546-0215, United States.
authors: Tammy
authors: T
authors: Brewster-Barnes
authors: Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546-0099, United States.
authors: Daniel K
authors: DK
authors: Howe
authors: Veterinary Diagnostic Laboratory, Department of Veterinary Science, University of Kentucky, Lexington, KY 40511, United States.
authors: Craig N
authors: CN
authors: Carter
authors: Veterinary Diagnostic Laboratory, Department of Veterinary Science, University of Kentucky, Lexington, KY 40511, United States.
authors: Uneeda K
authors: UK
authors: Bryant
authors: W.P. Garrigus Building, Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546-0215, United States.
authors: Mary G
authors: MG
authors: Rossano
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D017191:Ascaridida Infections / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D001199:Ascaridoidea / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D015331:Cohort Studies; D003126:Colostrum / Q000276:immunology; D005243:Feces / Q000469:parasitology; D005260:Female; D006734:Horse Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology*; D006736:Horses; D007112:Immunity, Maternally-Acquired; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007814:Larva / Q000276:immunology; D008297:Male; D010270:Parasite Egg Count / Q000662:veterinary
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G
keywords: Equine; Excretory-secretory; Horse; IgG(T); Parascaris equorum; Western blot