title: Multiplex method for initial complex testing of antibodies to blood transmitted diseases agents.
abstract: Initial screening of donors and population at high risk of infection with blood transmitted diseases involves a number of analyses using monospesific diagnostic systems, and therefore is expensive labor- and time-consuming process. The goal of this work is to construct a multiplex test enabling to carry out rapid initial complex testing at a low price. The paper describes a kit making it possible to detect simultaneously antibodies to six agents of the most significant blood transmitted diseases: HIV virus, hepatitis B and C viruses, cytomegalovirus, T. pallidum and T. gondii in blood products. The kit comprises multiplex dot-immunoassay based on plane protein arrays (immune chips) using colloidal gold conjugates and silver development. It provides an opportunity to carry out complex analysis within 70min at room temperature, and there is no need of well-qualified personnel. We compared laboratory findings of the kit with monospecific kits for ELISA produced by two Russian commercial companies. Dot-assay results correlate well with data obtained using commercial kits for ELISA. Furthermore, multiplex analysis is quicker and cheaper in comparison with ELISA and can be carried out in non-laboratory conditions. The kit for multiplex dot-immunoassay of antibodies to blood transmitted agents can significantly simplify initial complex testing.
authors: Vector State Research Center of Virology and Biotechnology, 630559, Koltsovo, Novosibirsk Region, Russia.
authors: Alexander G
authors: AG
authors: Poltavchenko
authors: Vector State Research Center of Virology and Biotechnology, 630559, Koltsovo, Novosibirsk Region, Russia.
authors: Oleg V
authors: OV
authors: Nechitaylo
authors: Vector State Research Center of Virology and Biotechnology, 630559, Koltsovo, Novosibirsk Region, Russia.
authors: Pavel V
authors: PV
authors: Filatov
authors: Vector State Research Center of Virology and Biotechnology, 630559, Koltsovo, Novosibirsk Region, Russia.
authors: Anna V
authors: AV
authors: Ersh
authors: Vector State Research Center of Virology and Biotechnology, 630559, Koltsovo, Novosibirsk Region, Russia. Electronic address: gureyev@ngs.ru.
authors: Vadim N
authors: VN
authors: Gureyev
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000913:Antibodies, Protozoan / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D003365:Costs and Cost Analysis; D003955:Diagnostic Tests, Routine / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods; D013587:Syphilis / Q000175:diagnosis*; D013995:Time; D014123:Toxoplasmosis / Q000175:diagnosis*; D014777:Virus Diseases / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D000913:Antibodies, Protozoan; D000914:Antibodies, Viral
keywords: Antibodies; Blood transmitted diseases; Initial complex testing; Multiplex dot-immunoassay