title: Intestinal trefoil factor increased the Bcl-2 level in a necrotizingenterocolitis neonate rat model.
abstract: OBJECTIVE
  The aim of this study was to investigate the therapeutic effect of intestinal trefoil factor (ITF) on necrotizing enterocolitis (NEC) by observing the pathological changes and detecting the protein level differences in Caspase-3, Bax, and Bcl-2 in an NEC neonate rat model.
  
  
  METHODS
  A Wistar rat model of NEC was established and 30 one-day-old neonate Wistar rats were randomly divided into three groups including a normal control (group A), NEC rats treated with 0.2 ml physiological saline through intraperitoneal (i.p.) injection (group B), and NEC rats treated with 0.2 mg ITF by i.p injection (group C).
  
  
  RESULTS
  Compared with group B, there were statistically significant differences in Caspase-3, Bax, and Bcl-2 levels in groups A and C(P < 0.05). Moreover, there was a significant difference in the Bcl-2 level between groups A and B (P < 0.05).
  
  
  CONCLUSIONS
  ITF alleviated injury of the intestinal tract in neonate rats with NEC and this mechanism was possibly related to a reduction in the expression of Caspase-3 and Bax and the increase in Bcl-2 expression.
authors: Department of Pediatrics, Hangzhou Normal University Affiliated Hospital, Hangzhou, China.
authors: Xiaolian
authors: X
authors: Yi
authors: Department of Pediatrics, Yuhang District Maternity and Child Care Centers of Hangzhou City, Hangzhou, China.
authors: Xiaojun
authors: X
authors: Chang
authors: Department of Pediatrics, Yuhang District Maternity and Child Care Centers of Hangzhou City, Hangzhou, China.
authors: Jijie
authors: J
authors: Wang
authors: Department of Pediatrics, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Caixia
authors: C
authors: Yan
authors: Department of Pediatrics, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Binghong
authors: B
authors: Zhang
mesh_terms: D000818:Animals; D020345:Enterocolitis, Necrotizing; D007422:Intestines; D051381:Rats; D017208:Rats, Wistar; D000071165:Trefoil Factor-3
chemical_list: D000071165:Trefoil Factor-3
keywords: Bax; Bcl-2; Intestinal trefoil factor; caspase-3; necrosis; neonate rat