title: Correlation between factor VII and PAI-1 genetic variants and recurrent miscarriage.
abstract: Polymorphisms which are presented below may be the cause of inherited thrombophilia and may result in pregnancy loss. The hypothesis is based on a number of cardiology studies which have confirmed the involvement of these polymorphisms in thrombotic incidents.
  
  
  
  To evaluate the role of polymorphisms of factor VII gene (Arg353Gln, -122T > C) and PAI-1 gene (-675 4G/5G) in the etiology of recurrent miscarriage.
  
  
  
  The study group included 152 women with a positive history of ≥ 2 consecutive pregnancy losses (114 and 38 women with 2 and ≥ 3 miscarriages, respectively), while 180 healthy women were recruited as controls. Genetic analysis was performed with the use of PCR/RFLP.
  
  
  
  Lower frequency of Arg353/Gln353 was observed in women with 2 and ≥ 3 miscarriages as compared to controls (21.1% vs. 23.9% and 13.2% vs. 23.9%, respectively). The frequency of Gln353 was lower in women with ≥ 3 miscarriages as compared to controls (6.6% vs. 11.9%, p = ns). The frequency of -122TT was higher in women with ≥ 3 miscarriages as compared to controls (86.84% vs. 76.67%, p = ns), whereas -122TC was more frequent in controls (13.16% vs. 22.78% in controls, p = ns). The frequency of -122T was higher in patients with ≥ 3 abortions as compared to controls (93.42% vs. 88.06%, p = ns), and -122C was observed more frequently in controls (6.58% vs. 11.94% in controls, p = ns). There were no significant differences as far as the -675 4G/5G polymorphism was concerned.
  
  
  
  The obtained results suggest a possible protective role of Gln353 and -122C alleles in recurrent miscarriage.
authors: Department of Perinatology and Women's Diseases, Poznan University of Medical Sciences, Poland Laboratory of Molecular Biology in Division of Perinatology and Women's Diseases, Poznan University of Medical Sciences, Poznan, Poland. magda.barlik@op.pl.
authors: Magdalena
authors: M
authors: Barlik
authors: Agnieszka
authors: A
authors: Seremak-Mrozikiewicz
authors: Krzysztof
authors: K
authors: Drews
authors: Andrzej
authors: A
authors: Klejewski
authors: Grażyna
authors: G
authors: Kurzawińska
authors: Zdzisław
authors: Z
authors: Łowicki
authors: Hubert
authors: H
authors: Wolski
mesh_terms: D000026:Abortion, Habitual / Q000209:etiology / Q000235:genetics; D000328:Adult; D005167:Factor VII / Q000235:genetics*; D005260:Female; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D011110:Polymorphism, Genetic; D011247:Pregnancy; D065840:Protective Factors; D019851:Thrombophilia / Q000150:complications / Q000235:genetics
chemical_list: D017395:Plasminogen Activator Inhibitor 1; C051321:SERPINE1 protein, human; D005167:Factor VII
keywords: gene polymorphism; pregnancy loss; thrombophilia