title: Fabrication of poly(ε-caprolactone)/keratin nanofibrous mats as a potential scaffold for vascular tissue engineering.
abstract: The natural abundance of cell adhesion sequences, RGD (Arg-Gly-Asp) and LDV (Leu-Asp-Val) in the keratins make them suitable as biomaterials for tissue engineering applications. Herein, keratins were coelectrospun with poly(ε-caprolactone)(PCL) at the ratio of 9/1, 8/2, and 7/3 to afford nanofibrous mats. The resulting mats were surface-characterized by ATR-FTIR, SEM, WCA, and XPS. Cell attachment data showed that NIH 3T3 cells adhered more to the PCL/keratin nanofibrous mats than the pristine PCL mats. The MTT assay revealed that the PCL/keratin mats had improved cell viability. The blood clotting time test (APTT, PT, and TT) indicated the PCL/keratin mats exerted good blood compatibility. These mats would be a good candidate as a scaffold for vascular tissue engineering.
authors: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
authors: Yanmei
authors: Y
authors: Li
authors: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
authors: Yanfang
authors: Y
authors: Wang
authors: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
authors: Jingjie
authors: J
authors: Ye
authors: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China. Electronic address: jyuan@njnu.edu.cn.
authors: Jiang
authors: J
authors: Yuan
authors: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China. Electronic address: yhxiao@njnu.edu.cn.
authors: Yinghong
authors: Y
authors: Xiao
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001807:Blood Vessel Prosthesis; D002470:Cell Survival / Q000187:drug effects; D007633:Keratins / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D007633:Keratins
keywords: Blood compatibility; Cytocompatibility; Electrospinning; Keratin; Poly(ε-caprolactone)