title: Flow Cytometry in Detection of Fetal Red Blood Cells and Maternal F Cells to Identify Fetomaternal Hemorrhage.
abstract: Accurate detection and quantitation of fetomaternal hemorrhage (FMH) is critical to the obstetric management of rhesus D alloimmunization in Rh-negative pregnant women. The flow cytometry is based on the detection of fetal red blood cells using a monoclonal anti-HbF antibody, and is the method most indicated for this estimation. The objective of this study was to quantify fetal red blood cell levels of pregnant women using flow cytometry. We analyzed 101 peripheral blood samples from Rh-negative and Rh-positive women, whose mean age was 24 years (20-32 years), after vaginal delivery or cesarean section. Our study showed that 53% of pregnant women had fetal red blood cells levels <2.0 mL, 31% between 2.0-3.9 mL, 16% between 4.0-15.0 mL, and 1% >15.0 mL. Accurate quantitation of fetal red blood cells is necessary to determine the appropriate dose of anti-D (RHD) immunoglobulin to be administered to pregnant or postpartum women.
authors: a Department of Clinical Pathology , Flow Cytometry, Hospital de Clínicas de Porto Alegre , Porto Alegre , Brazil.
authors: Mariela Granero
authors: MG
authors: Farias
authors: b Department of Clinical Pathology , Hematology Unit, Hospital de Clínicas de Porto Alegre , Porto Alegre , Brazil.
authors: Suzane
authors: S
authors: Dal Bó
authors: c Universidade Federal do Rio Grande do Sul , School of Pharmacy , Porto Alegre , Brazil.
authors: Simone Martins de
authors: SM
authors: Castro
authors: d Hematology , Hospital de Clinicas de Porto Alegre , Porto Alegre , Brazil.
authors: Aline Reis
authors: AR
authors: da Silva
authors: c Universidade Federal do Rio Grande do Sul , School of Pharmacy , Porto Alegre , Brazil.
authors: Joyce
authors: J
authors: Bonazzoni
authors: b Department of Clinical Pathology , Hematology Unit, Hospital de Clínicas de Porto Alegre , Porto Alegre , Brazil.
authors: Luciana
authors: L
authors: Scotti
authors: e Department of Obstetrics and Gynecology , Hospital de Clinicas de Porto Alegre , Porto Alegre , Brazil.
authors: Sergio H Almeida Martins
authors: SH
authors: Costa
mesh_terms: D000328:Adult; D001787:Blood Group Incompatibility / Q000503:physiopathology; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005319:Fetal Hemoglobin / Q000378:metabolism; D005331:Fetomaternal Transfusion / Q000175:diagnosis* / Q000628:therapy; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007231:Infant, Newborn; D049590:Postpartum Period / Q000502:physiology; D011247:Pregnancy; D012204:Rh-Hr Blood-Group System / Q000502:physiology; D018029:Rho(D) Immune Globulin / Q000627:therapeutic use; D055815:Young Adult
chemical_list: C061961:RHO(D) antibody; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin; D005319:Fetal Hemoglobin
keywords: Fetal hemoglobin; Rh; alloimmunization; flow cytometry