title: Beta-HPV types in patients with head and neck pathology and in healthy subjects.
abstract: Human papillomaviruses (HPV) are a heterogeneous group of viruses classified into five genera. The beta-HPV type (beta-PV) infection is very common but mostly asymptomatic in immunocompetent individuals. However, beta-PVs play a role in Epidermodysplasia verruciformis and possibly in non-melanoma skin cancer. Head and neck cancer (HNC) is a common cancer type worldwide and high-risk alpha-PV involvement in HNC has been extensively studied but beta-PV types have rarely been the focus of such studies.
  
  
  
  To evaluate the prevalence of beta-PV types in HNC, subjects with non-malignant or potentially pre-malignant oral lesions, and healthy controls.
  
  
  
  The frequency of different beta-PVs in samples from oral (n=35) and oropharyngeal (n=35) cancer patients, gender- and age-matched healthy controls (n=70), and subjects with various non-malignant or potentially pre-malignant oral lesions (n=102) was assessed by a highly sensitive, bead-based, multiplex genotyping assay.
  
  
  
  Overall, 54.8% of all tested samples contained at least one beta-PV type. Even though the correlation between types found in lavage and tissue specimens from cancer patients was low, there was a large statistically significant difference between oropharyngeal cancer patients and matched controls for HPV5 (P=0.003; OR=15.58) and between both oral (P=0.026; OR=5.7) and oropharyngeal cancer patients (P=0.002; OR=25.5) and controls for HPV122. In addition, there was no correlation between the prevalence of alpha and beta-PVs in the study patients.
  
  
  
  The study provides new data on the prevalence of beta-PVs in HNC. HPV5 was found significantly associated with HNC as already observed by other studies. Additionally, the significant association of HPV122 with HNC might warrant further study as this type has not been extensively studied so far.
authors: Department of Immunology, Institute of Hematology and Blood Transfusion, Prague, Czech Republic; Department of Molecular Medicine, Ruđer Bošković Institute, Zagreb, Croatia.
authors: Ivan
authors: I
authors: Sabol
authors: Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Czech Republic.
authors: Jana
authors: J
authors: Smahelova
authors: Department of Otorhinolaryngology and Head and Neck Surgery, First Faculty of Medicine, Charles University in Prague, Motol University Hospital, Prague, Czech Republic.
authors: Jan
authors: J
authors: Klozar
authors: Department of Oral Medicine, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.
authors: Marinka
authors: M
authors: Mravak-Stipetic
authors: Infections and Cancer Biology Group, International Agency for Research on Cancer - World Health Organization, Lyon, France.
authors: Tarik
authors: T
authors: Gheit
authors: Infections and Cancer Biology Group, International Agency for Research on Cancer - World Health Organization, Lyon, France.
authors: Massimo
authors: M
authors: Tommasino
authors: Department of Molecular Medicine, Ruđer Bošković Institute, Zagreb, Croatia.
authors: Magdalena
authors: M
authors: Grce
authors: Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Czech Republic. Electronic address: Ruth.Tachezy@uhkt.cz.
authors: Ruth
authors: R
authors: Tachezy
mesh_terms: D000328:Adult; D000368:Aged; D052686:Betapapillomavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000821:virology; D009059:Mouth Diseases / Q000453:epidemiology / Q000821:virology*; D009960:Oropharynx / Q000821:virology; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D015995:Prevalence
keywords: Cancer; Lesion; Oral; Oropharyngeal; alpha-HPV; beta-HPV