title: A draft genome of the brown alga, Cladosiphon okamuranus, S-strain: a platform for future studies of 'mozuku' biology.
abstract: The brown alga, Cladosiphon okamuranus (Okinawa mozuku), is economically one of the most important edible seaweeds, and is cultivated for market primarily in Okinawa, Japan. C. okamuranus constitutes a significant source of fucoidan, which has various physiological and biological activities. To facilitate studies of seaweed biology, we decoded the draft genome of C. okamuranus S-strain. The genome size of C. okamuranus was estimated as ∼140 Mbp, smaller than genomes of two other brown algae, Ectocarpus siliculosus and Saccharina japonica Sequencing with ∼100× coverage yielded an assembly of 541 scaffolds with N50 = 416 kbp. Together with transcriptomic data, we estimated that the C. okamuranus genome contains 13,640 protein-coding genes, approximately 94% of which have been confirmed with corresponding mRNAs. Comparisons with the E. siliculosus genome identified a set of C. okamuranus genes that encode enzymes involved in biosynthetic pathways for sulfated fucans and alginate biosynthesis. In addition, we identified C. okamuranus genes for enzymes involved in phlorotannin biosynthesis. The present decoding of the Cladosiphon okamuranus genome provides a platform for future studies of mozuku biology.
authors: Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan koki.nishitsuji@oist.jp eiichi@oist.jp.
authors: Koki
authors: K
authors: Nishitsuji
authors: Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.
authors: Asuka
authors: A
authors: Arimoto
authors: Okinawa Prefectural Fisheries Research and Extension Center, Itoman, Okinawa 901-0354, Japan.
authors: Kenji
authors: K
authors: Iwai
authors: Okinawa Prefectural Fisheries Research and Extension Center, Itoman, Okinawa 901-0354, Japan.
authors: Yusuke
authors: Y
authors: Sudo
authors: Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.
authors: Kanako
authors: K
authors: Hisata
authors: DNA Sequencing Section, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.
authors: Manabu
authors: M
authors: Fujie
authors: DNA Sequencing Section, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.
authors: Nana
authors: N
authors: Arakaki
authors: School of Agriculture, Meiji University, Kawasaki, Kanagawa 214-8571, Japan.
authors: Tetsuo
authors: T
authors: Kushiro
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan.
authors: Teruko
authors: T
authors: Konishi
authors: Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.
authors: Chuya
authors: C
authors: Shinzato
authors: Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.
authors: Noriyuki
authors: N
authors: Satoh
authors: Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan koki.nishitsuji@oist.jp eiichi@oist.jp.
authors: Eiichi
authors: E
authors: Shoguchi
mesh_terms: D016678:Genome; D058977:Molecular Sequence Annotation; D016366:Open Reading Frames; D000459:Phaeophyceae / Q000235:genetics*; D011134:Polysaccharides / Q000235:genetics / Q000378:metabolism; D012624:Seaweed / Q000235:genetics*; D059467:Transcriptome
chemical_list: D011134:Polysaccharides
keywords: Cladosiphon okamuranus; brown alga; genes for enzymes of polysaccharide synthesis; genome decoding