title: Reconstitution of intracellular environments in vitro and in artificial cells.
abstract: Toward reconstitution of living cells by artificial cells technology, it is critical process to understand the differences between mixtures of biomolecules and living cells. For the aim, we have developed procedures for preparation of an additive-free cell extract (AFCE) and for concentrating biomacromolecules in artificial cells. In this review, we introduce our recent progress to reconstitute intracellular environments in vitro and in artificial cells.
authors: Department of Biosciences & Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors: Kei
authors: K
authors: Fujiwara
authors: Department of Applied Physics, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors: Miho
authors: M
authors: Yanagisawa
authors: Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
authors: Shin-Ichiro M
authors: SM
authors: Nomura
keywords: Liposome; cell reconstitution; cell-free protein expression; molecular crowding