title: Analyses of effects of α-cembratrien-diol on cell morphology and transcriptome of Valsa mali var. mali.
abstract: The objective of this work was to study the underlying mechanisms of growth inhibition of Valsa mali var. mali, the causative pathogen of apple tree canker disease, by α-cembratrien-diol. The half maximal inhibitory concentration of α-cembratrien-diol against V. mali var. mali is 18.0mg/L. Treatment of V. mali var. mali with α-cembratrien-diol resulted in various mycelial and cellular abnormalities, and the up- and down-regulation of 94 and 170 differentially expressed genes, respectively. Gene Ontology term enrichment analysis revealed that α-cembratrien-diol substantially altered the expression of genes involved in the redox process, tetrapyrrole binding, coenzyme binding, heme binding, and iron binding. Kyoto Encyclopedia of Genes and Genomes enrichment analysis also showed significant enrichment of specific metabolic pathways involving the set of differentially expressed genes. The present study will assist in the development of alternative α-cembratrien-diol-based biological control agents and ultimately facilitate organic apple production.
authors: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
authors: Ning
authors: N
authors: Yan
authors: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
authors: Yongmei
authors: Y
authors: Du
authors: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
authors: Xinmin
authors: X
authors: Liu
authors: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
authors: Hongbo
authors: H
authors: Zhang
authors: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
authors: Yanhua
authors: Y
authors: Liu
authors: Guelph Food Research Center, Agriculture and Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada.
authors: John
authors: J
authors: Shi
authors: Guelph Food Research Center, Agriculture and Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada.
authors: Sophia Jun
authors: SJ
authors: Xue
authors: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China. Electronic address: 13646810352@163.com.
authors: Zhongfeng
authors: Z
authors: Zhang
mesh_terms: D001203:Ascomycota / Q000166:cytology / Q000187:drug effects* / Q000235:genetics; D048430:Cell Shape / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D059467:Transcriptome / Q000187:drug effects*
chemical_list: C569865:(1S,2E,4R,6R,7E,11E)-2,7,11-cembratriene-4,6-diol; D004224:Diterpenes
keywords: Mycelial morphology; RNA-Seq; Ultrastructure; Valsa mali var. mali; α-cembratrien-diol