title: Paper-based fluorescent sensor for rapid naked-eye detection of acetylcholinesterase activity and organophosphorus pesticides with high sensitivity and selectivity.
abstract: Various strategies have been proposed for the sensing of acetylcholinesterase (AChE) activity and organophosphorus pesticides (OPs). However, the practical application of most methods is restricted by their intrinsic drawbacks such as complexity, long analysis time, and high cost. Thus, it is highly desirable to develop simple, fast and sensitive approaches for AChE activity and OPs detection. Herein, we reported a simple paper-based fluorescent sensor (PFS) based on the aggregation induced emission (AIE) effect of tetraphenylethylene (TPE) and the addition reaction capability of maleimide, which has been used as a powerful tool for rapid naked-eye detection of AChE activity and OPs. The introduction of TPE provides the probe with unique fluorescence property in solid state and is of great importance for improving the sensitivity of PFS. The hydrolysis product of acetylthiocholine catalyzed by AChE induced the maleimide ring destruction and activated the fluorescence performance of TPE. Given that AChE activity can be specifically inhibited by OPs, the as-proposed PFS can also be utilized for sensitive detection of OPs. Meanwhile, the variation of fluorescence signal can be readily detected by naked eyes, and low detection limits of 2.5mUmL(-1) and 0.5ngmL(-1) for AChE activity and OPs are obtained, respectively. Moreover, it has been successfully applied for AChE activity and OPs detection in diluted human serum samples, showing its great potential to be applied in real samples. Thus, this strategy possesses considerable advantages of simplicity, rapid detection, portability, cost efficiency and visualization.
authors: College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, People's Republic of China.
authors: Jiafu
authors: J
authors: Chang
authors: College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, People's Republic of China.
authors: Haiyin
authors: H
authors: Li
authors: College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, People's Republic of China.
authors: Ting
authors: T
authors: Hou
authors: College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, People's Republic of China. Electronic address: lifeng@qust.edu.cn.
authors: Feng
authors: F
authors: Li
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis / Q000737:chemistry*; D003124:Colorimetry / Q000295:instrumentation*; D004209:Disposable Equipment; D004784:Environmental Monitoring / Q000295:instrumentation*; D004789:Enzyme Activation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000737:chemistry; D010209:Paper; D010575:Pesticides / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
chemical_list: D009943:Organophosphorus Compounds; D010575:Pesticides; D000110:Acetylcholinesterase
keywords: Acetylcholinesterase; Aggregation induced emission; Naked-eye detection; Organophosphorus pesticides; Paper-based fluorescent sensor