title: Contributions of Mammalian Chimeras to Pluripotent Stem Cell Research.
abstract: Chimeras are widely acknowledged as the gold standard for assessing stem cell pluripotency, based on their capacity to test donor cell lineage potential in the context of an organized, normally developing tissue. Experimental chimeras provide key insights into mammalian developmental mechanisms and offer a resource for interrogating the fate potential of various pluripotent stem cell states. We highlight the applications and current limitations presented by intra- and inter-species chimeras and consider their future contribution to the stem cell field. Despite the technical and ethical demands of experimental chimeras, including human-interspecies chimeras, they are a provocative resource for achieving regenerative medicine goals.
authors: British Heart Foundation Oxbridge Centre for Regenerative Medicine, University of Cambridge, Cambridge, CB2 0SZ, UK; Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, CB2 0SZ, UK. Electronic address: vlm37@cam.ac.uk.
authors: Victoria L
authors: VL
authors: Mascetti
authors: Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, CB2 0SZ, UK; Department of Paediatrics, University of Cambridge, Cambridge, CB2 0SZ, UK. Electronic address: ralp2@cam.ac.uk.
authors: Roger A
authors: RA
authors: Pedersen
mesh_terms: D000818:Animals; D019070:Cell Lineage; D002678:Chimera / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology*; D013045:Species Specificity; D057905:Stem Cell Research