title: Pseudoduganella danionis sp. nov., isolated from zebrafish (Danio rerio).
abstract: One beige-pigmented, Gram-staining-negative, rod-shaped bacterium, strain E3/2T, was isolated from a zebrafish, Daniorerio. Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the isolate shared 97.7 % 16S rRNA gene sequence similarity to the species Pseudoduganella violaceinigra and between 97.4 to 97.0 % to some species of the genera Duganella and Massilia, including Duganella radicis, Duganella phyllosphaerae, Massilia dura, Massilia lutea, Duganella sacchari, Duganella zoogloeoides, Massiliaalbidiflava and Massilia umbonata. Sequence similarities to all other species were below 97 %. The main cellular fatty acids of the strain were summed feature 3 fatty acids (C16 : 1ω7c/iso-C15 : 0 2-OH), C10 : 0 3-OH, C16 : 0 and C12 : 0. The polyamine pattern of strain E3/2T contained predominantly putrescine and 2-hydroxyputrescine. The major quinone was ubiquinone Q-8. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Based on phylogenetic, chemotaxonomic, genomic and phenotypic analyses we propose a novel species of the genus Pseudoduganella named Pseudoduganella danionis sp. nov., with strain E3/2T (=LMG 29678T=CCM 8698T) as the type strain.
authors: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Giessen D-35392, Germany.
authors: Peter
authors: P
authors: Kämpfer
authors: Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Viña del Mar, Chile.|Interdisciplinary Center for Aquaculture Research (INCAR), Concepción, Chile.
authors: Rute
authors: R
authors: Irgang
authors: Institut für Mikrobiologie, Veterinärmedizinische Universität Wien, Wien A-1210, Austria.
authors: Hans-Jürgen
authors: HJ
authors: Busse
authors: Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Viña del Mar, Chile.|Interdisciplinary Center for Aquaculture Research (INCAR), Concepción, Chile.
authors: Mathías
authors: M
authors: Poblete-Morales
authors: Institut für Mikrobiologie, Veterinärmedizinische Universität Wien, Wien A-1210, Austria.
authors: Tanita
authors: T
authors: Kleinhagauer
authors: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Giessen D-35392, Germany.
authors: Stefanie P
authors: SP
authors: Glaeser
authors: Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Viña del Mar, Chile.|Interdisciplinary Center for Aquaculture Research (INCAR), Concepción, Chile.
authors: Ruben
authors: R
authors: Avendaño-Herrera
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D042802:Oxalobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010802:Phylogeny; D011700:Putrescine / Q000031:analogs & derivatives / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D015027:Zebrafish / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C483858:phosphatidylethanolamine; C002952:2-hydroxyputrescine; C030778:ubiquinone 8; D011700:Putrescine