title: The effect of low-dose methotrexate on autologous fat graft survival.
abstract: OBJECTIVE
  The survival of autologous fat graft tissue is dependent on various factors such as vascularization and inflammation. The aim of the present study was to evaluate the possible beneficial effects of low-dose methotrexate (LD-MTX) on fat graft volume and survival.
  
  
  METHODS
  A total of 13 male Wistar albino rats were divided into two groups, a control group and an LD-MTX group. An autologous fat graft obtained from the inguinal region of each rat was transferred to its back. LD-MTX was administered intraperitoneally in the LD-MTX group once a week for 4 weeks after the surgical procedure. The control group underwent surgery but was not administered MTX. Fat grafts were harvested for analyses.
  
  
  RESULTS
  The results showed that 2 months postoperatively the fat graft weights of the control and LD-MTX groups were not significantly different. In addition, the vascularity of the grafts was higher in the LD-MTX group than it was in the control group. The mean lipid peroxidation levels were essentially the same in the two groups, but myeloperoxidation was significantly lower in the LD-MTX group than it was in the other group.
  
  
  CONCLUSIONS
  The results showed that LD-MTX administration may not preserve the quality and volume of transplanted fat tissue in rats.
authors: Department of Plastic, Reconstructive, and Aesthetic Surgery, Faculty of Medicine, Kırıkkale University, Kırıkkale, Turkey.
authors: Elif
authors: E
authors: Sari
authors: Department of Neurosurgery, Faculty of Medicine, Kırıkkale University, Kırıkkale, Turkey.
authors: Bülent
authors: B
authors: Bakar
authors: Department of Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors: Bahram
authors: B
authors: Sarkarati
authors: Department of Pathology, Faculty of Medicine, Kırıkkale University, Kırıkkale, Turkey.
authors: Önder
authors: Ö
authors: Bozdoğan
authors: Plastic, Reconstructive, and Aesthetic Surgery Clinic, Memorial Hospital, Ankara, Turkey.
authors: Tarık
authors: T
authors: Çavuşoğlu
mesh_terms: D000818:Animals; D005223:Fats; D006085:Graft Survival; D007249:Inflammation; D015227:Lipid Peroxidation; D008297:Male; D008727:Methotrexate; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005223:Fats; D008727:Methotrexate
keywords: Fat graft; fat graft survival; methotrexate