title: The class III peroxidase PRX17 is a direct target of the MADS-box transcription factor AGAMOUS-LIKE15 (AGL15) and participates in lignified tissue formation.
abstract: Several physiological functions have been attributed to class III peroxidases (PRXs) in plants, but the in planta role of most members of this family still remains undetermined. Here, we report the first functional characterization of PRX17 (At2g22420), one of the 73 members of this family in Arabidopsis thaliana. Localization of PRX17 was examined by transient expression in Nicotiana benthamiana. Loss- and gain-of-function mutants in A. thaliana were studied. Regulation at the gene and protein levels was analyzed using β-glucuronidase (GUS) activity, quantitative reverse transcriptase (qRT)-PCR, zymography, and chromatin immunoprecipitation. Phenotypes were characterized including lignin and xylan contents. PRX17 was expressed in various tissues, including vascular tissues, and PRX17 was localized to the cell wall. In prx17, the lignin content was reduced in the stem and siliques and bolting was delayed, while the opposite phenotype was observed in 35S:PRX17 plants, together with a significant increase of lignin and xylan immunofluorescence signal. Finally, we demonstrated that the transcription factor AGAMOUS-LIKE15 (AGL15) binds to the PRX17 promoter and regulates PRX17 expression level. This converging set of structural, transcriptomic and physiological data suggests that PRX17, under the control of AGL15, contributes to developmental programs by playing an essential role in regulating age-dependent lignified tissue formation, including changes in cell wall properties.
authors: Division of Biological Sciences, Section Cell & Developmental Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
authors: Claudia
authors: C
authors: Cosio
authors: Laboratoire de Recherche en Sciences Végétales, CNRS, UPS, Université de Toulouse, 24 chemin de Borde Rouge, Auzeville, BP42617, Castanet Tolosan, 31326, France.
authors: Philippe
authors: P
authors: Ranocha
authors: Laboratoire de Recherche en Sciences Végétales, CNRS, UPS, Université de Toulouse, 24 chemin de Borde Rouge, Auzeville, BP42617, Castanet Tolosan, 31326, France.
authors: Edith
authors: E
authors: Francoz
authors: Laboratoire de Recherche en Sciences Végétales, CNRS, UPS, Université de Toulouse, 24 chemin de Borde Rouge, Auzeville, BP42617, Castanet Tolosan, 31326, France.
authors: Vincent
authors: V
authors: Burlat
authors: Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, 40546-0091, USA.
authors: Yumei
authors: Y
authors: Zheng
authors: Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, 40546-0091, USA.
authors: Sharyn E
authors: SE
authors: Perry
authors: Division of Biological Sciences, Section Cell & Developmental Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
authors: Juan-Jose
authors: JJ
authors: Ripoll
authors: Division of Biological Sciences, Section Cell & Developmental Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
authors: Martin
authors: M
authors: Yanofsky
authors: Laboratoire de Recherche en Sciences Végétales, CNRS, UPS, Université de Toulouse, 24 chemin de Borde Rouge, Auzeville, BP42617, Castanet Tolosan, 31326, France.
authors: Christophe
authors: C
authors: Dunand
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002473:Cell Wall / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D035264:Flowers / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008031:Lignin / Q000378:metabolism*; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D009195:Peroxidase / Q000378:metabolism*; D010544:Peroxidases; D010802:Phylogeny; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: C522473:AGL15 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D026161:MADS Domain Proteins; D012333:RNA, Messenger; C036483:T-DNA; D008031:Lignin; D010544:Peroxidases; C000626243:PRX17 protein, Arabidopsis; D009195:Peroxidase
keywords: 
  Arabidopsis thaliana
  ; chromatin immunoprecipitation-with DNA microarray (ChIP-chip); development; flower; isoelectric focusing (IEF); peroxidase; silique; stem