title: WNT2 Promotes Cervical Carcinoma Metastasis and Induction of Epithelial-Mesenchymal Transition.
abstract: BACKGROUND
  Previously, we found an 11-gene signature could predict pelvic lymph node metastasis (PLNM), and WNT2 is one of the key genes in the signature. This study explored the expression and underlying mechanism of WNT2 in PLNM of cervical cancer.
  
  
  METHODS
  WNT2 expression level in cervical cancer was detected using western blotting, quantitative PCR, and immunohistochemistry. Two WNT2-specific small interfering RNAs (siRNAs) were used to explore the effects of WNT2 on invasive and metastatic ability of cancer cells, and to reveal the possible mechanism of WNT2 affecting epithelial-mesenchymal transition (EMT). The correlation between WNT2 expression and PLNM was further investigated in clinical cervical specimens.
  
  
  RESULTS
  Both WNT2 mRNA and protein expression was upregulated in cervical cancer. High WNT2 expression was significantly associated with tumor size, lymphovascular space involvement, positive parametrium, and most importantly, PLNM. PLNM and WNT2 expression were independent prognostic factors for overall survival and disease-free survival. WNT2 knockdown inhibited SiHa cell motility and invasion and reversed EMT by inhibiting the WNT2/β-catenin pathway. WNT2 overexpression in cervical cancer was associated with β-catenin activation and induction of EMT, which further contributed to metastasis in cervical cancer.
  
  
  CONCLUSIONS
  WNT2 might be a novel predictor of PLNM and a promising prognostic indicator in cervical cancer.
authors: Department of Gynecology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.
authors: Yun
authors: http://orcid.org/0000-0002-6255-301X
authors: Y
authors: Zhou
authors: Department of Gynecology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.
authors: Yongwen
authors: Y
authors: Huang
authors: Department of Radiotherapy, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.
authors: Xinping
authors: X
authors: Cao
authors: State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.
authors: Jing
authors: J
authors: Xu
authors: Department of Gynecology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.
authors: Lan
authors: L
authors: Zhang
authors: Cardiovascular Department, Second People's Hospital of Guangdong Province, 1 Shi-liu gang Road East, Guangzhou, 510317, P. R. China.
authors: Jianhua
authors: J
authors: Wang
authors: Department of Oncology, the Second Affiliated Hospital, Nanchang University, Nanchang, 330000, P. R. China.
authors: Long
authors: L
authors: Huang
authors: Department of Gynecology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.
authors: Shuting
authors: S
authors: Huang
authors: Department of Gynecology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.
authors: Linjing
authors: L
authors: Yuan
authors: State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.
authors: Weihua
authors: W
authors: Jia
authors: State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.
authors: Xingjuan
authors: X
authors: Yu
authors: Department of Pathology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.
authors: Rongzhen
authors: R
authors: Luo
authors: Department of Gynecology, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.|Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou, Guangzhou, 510060, P. R. China.
authors: Min
authors: M
authors: Zheng
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D016022:Case-Control Studies; D002465:Cell Movement; D015331:Cohort Studies; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D010386:Pelvic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D011379:Prognosis; D015996:Survival Rate; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051154:Wnt2 Protein / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C495244:WNT2 protein, human; D051154:Wnt2 Protein; D051176:beta Catenin