title: Human lung epithelial cell A549 proteome data after treatment with titanium dioxide and carbon black.
abstract: Here, we have described the dataset relevant to the A549 cellular proteome changes after exposure to either titanium dioxide or carbon black particles as compared to the non-exposed controls, "Proteomic changes in human lung epithelial cells (A549) in response to carbon black and titanium dioxide exposures" (Vuong et al., 2016) [1]. Detailed methodologies on the separation of cellular proteins by 2D-GE and the subsequent mass spectrometry analyses using MALDI-TOF-TOF-MS are documented. Particle exposure-specific protein expression changes were measured via 2D-GE spot volume analysis. Protein identification was done by querying mass spectrometry data against SwissProt and RefSeq protein databases using Mascot search engine. Two-way ANOVA analysis data provided information on statistically significant A549 protein expression changes associated with particle exposures.
authors: Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9; Department of Biochemistry, Faculty of Science, University of Ottawa, Ottawa, ON, Canada K1H 8M5.
authors: Ngoc Q
authors: NQ
authors: Vuong
authors: Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9.
authors: Patrick
authors: P
authors: Goegan
authors: Analytical Biochemistry and Proteomics, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9.
authors: Susantha
authors: S
authors: Mohottalage
authors: Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9.
authors: Dalibor
authors: D
authors: Breznan
authors: Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9.
authors: Marianne
authors: M
authors: Ariganello
authors: Biostatistics Section, Population Studies Division, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9.
authors: Andrew
authors: A
authors: Williams
authors: Analytical Biochemistry and Proteomics, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9.
authors: Fred
authors: F
authors: Elisma
authors: Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9.
authors: Subramanian
authors: S
authors: Karthikeyan
authors: Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9; Department of Biochemistry, Faculty of Science, University of Ottawa, Ottawa, ON, Canada K1H 8M5.
authors: Renaud
authors: R
authors: Vincent
authors: Analytical Biochemistry and Proteomics, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9; Interdisciplinary School of Health Sciences, Faculty of Health Sciences, University of Ottawa, Ottawa, ON, Canada K1H 8M5.
authors: Premkumari
authors: P
authors: Kumarathasan
keywords: 2D-GE; A549; Carbon black; MALDI-TOF-TOF-MS; Proteomics; Titanium dioxide; Toxicity