title: Short-term storage of salmonids semen in a sodium alginate-based extender.
abstract: Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our study was to analyse the effect of a sodium alginate-based extender on sperm function in the short-term storage of salmonids semen. Samples of Salmo salar, Oncorhynchus kisutch, and Oncorhynchus mykiss were stored in Storfish®(Ext-C) and Storfish®supplemented with sodium alginate (Ext-A) during 10 days at 4°C. After storage, motility, viability, mitochondrial membrane potential (ΔΨmit), superoxide anion (O2-) level and DNA fragmentation (DNA Frag) were assessed. Ext-A had positive effect in preservation of sperm motility, viability, ΔΨmit, O2-level and DNA integrity in the three species analysed compared to control samples. In Ext-A, the spermatozoa of S. salar and O. mykiss showed significantly higher motility, viability and ΔΨmit than O. kisutch. However, O. kisutch and O. mykiss had significantly lower O2-level than S. salar, and DNA fragmentation in O. kisutch and S. salar was significantly lower than in samples of O. mykiss (p < 0.05). Dilution of salmonids semen in a sodium alginate-based extender is effective for protecting sperm quality during 10 days of short-term storage.
authors: Center of Biotechnology in Reproduction (CEBIOR-BIOREN), Universidad de La Frontera, Temuco, Chile.|Doctoral Program in Morphological Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.
authors: O
authors: O
authors: Merino
authors: School of Aquaculture, Universidad Católica de Temuco, Temuco, Chile.|Department of Chemical Engineering, Faculty of Engineering and Science, Universidad de la Frontera, Temuco, Chile.
authors: E
authors: E
authors: Figueroa
authors: Center of Biotechnology in Reproduction (CEBIOR-BIOREN), Universidad de La Frontera, Temuco, Chile.
authors: C
authors: C
authors: Cheuquemán
authors: School of Aquaculture, Universidad Católica de Temuco, Temuco, Chile.
authors: I
authors: I
authors: Valdebenito
authors: Department of Obstetrics and Gynecology, University of Cologne, Cologne, Germany.
authors: V
authors: V
authors: Isachenko
authors: Department of Obstetrics and Gynecology, University of Cologne, Cologne, Germany.
authors: E
authors: E
authors: Isachenko
authors: Center of Biotechnology in Reproduction (CEBIOR-BIOREN), Universidad de La Frontera, Temuco, Chile.|Department Preclinical Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.
authors: R
authors: R
authors: Sánchez
authors: Center of Biotechnology in Reproduction (CEBIOR-BIOREN), Universidad de La Frontera, Temuco, Chile.|Department of Chemical Engineering, Faculty of Engineering and Science, Universidad de la Frontera, Temuco, Chile.
authors: J
authors: J
authors: Farías
authors: Center of Biotechnology in Reproduction (CEBIOR-BIOREN), Universidad de La Frontera, Temuco, Chile.|Department of Basic Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.
authors: J
authors: J
authors: Risopatrón
mesh_terms: D000464:Alginates; D000818:Animals; D002470:Cell Survival; D053938:DNA Fragmentation; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008297:Male; D053078:Membrane Potential, Mitochondrial; D012487:Salmonidae; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013481:Superoxides / Q000032:analysis; D013997:Time Factors
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D013481:Superoxides; D020723:Glucuronic Acid
keywords: antioxidants; fish; semen storage; sodium alginate; sperm function