title: Neuronal maturation in the hippocampal dentate gyrus via chronic oral administration ofArtemisa annuaextract is independent of cyclooxygenase 2 signaling pathway in diet-induced obesity mouse model.
abstract: Recently, we reported thatArtemisia annua(AA) has anti-adipogenic propertiesin vitroand invivo. Reduction of adipogenesis by AA treatment may dampen systemic inflammation and protect neurons from cytokine-induced damage. Therefore, the present study was undertaken to assess whether AA increases neuronal maturation by reducing inflammatory responses, such as those mediated by cyclooxygenase 2 (COX-2). Mice were fed normal chow or a high-fat diet with or without chronic daily oral administration of AA extract (0.2 g/10 mL/kg) for 4 weeks; then, changes in their hippocampal dentate gyri were measured via immunohistochemistry/immunofluorescence staining for bromodexoxyuridine, doublecortin, and neuronal nuclei, markers of neuronal maturation, and quantitative western blotting for COX-2 and Iba-1, in order to assess correlations between systemic inflammation (interleukin-6) and food type. Additionally, we tested the effect of AA in an Alzheimer's disease model ofCaenorhabditis elegansand uncovered a potential benefit. The results show that chronic AA dosing significantly increases neuronal maturation, particularly in the high-fat diet group. This effect was seen in the absence of any changes in COX-2 levels in mice given the same type of food, pointing to the possibility of alternate anti-inflammatory pathways in the stimulation of neurogenesis and neuro-maturation in a background of obesity.
authors: Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors: Hye Kyung
authors: HK
authors: Baek
authors: Biocenter, Gyeonggi Institute of Science and Technology Promotion (GSTEP), Suwon 16229, Korea.
authors: Pan Soo
authors: PS
authors: Kim
authors: Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors: Ji Ae
authors: JA
authors: Song
authors: Biocenter, Gyeonggi Institute of Science and Technology Promotion (GSTEP), Suwon 16229, Korea.
authors: Dong-Hwa
authors: DH
authors: Choi
authors: Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors: Do Eun
authors: DE
authors: Kim
authors: Department of Medical Biotechnology, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors: Seung Il
authors: SI
authors: Oh
authors: Department of Medical Biotechnology, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors: Sang-Kyu
authors: SK
authors: Park
authors: Department of Biotechnology, Hoseo University, Asan 31499, Korea.
authors: Sung-Jo
authors: SJ
authors: Kim
authors: Department of Animal Biotechnology, College of Agricultural Life Science, ChonBuk National University, Jeonju 54896, Korea.
authors: Ki-Duk
authors: KD
authors: Song
authors: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors: In Koo
authors: IK
authors: Hwang
authors: Department of Health Science, Konyang University, Nonsan 32992, Korea.
authors: Hyung Seok
authors: HS
authors: Seo
authors: Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors: Sun Shin
authors: SS
authors: Yi
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000284:Administration, Oral; D000818:Animals; D031172:Artemisia annua; D002454:Cell Differentiation / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000187:drug effects*; D018891:Dentate Gyrus / Q000187:drug effects*; D059305:Diet, High-Fat / Q000009:adverse effects / Q000662:veterinary; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects*; D009765:Obesity / Q000378:metabolism / Q000662:veterinary*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D051546:Cyclooxygenase 2
keywords: Artemisia annua; anti-obesity; cyclooxygenase 2; neuro-maturation; neurogenesis