title: Using L-STM to directly visualize enzymatic self-assembly/disassembly of nanofibers.
abstract: Self-assembly/disassembly is ubiquitous in nature and plays an important role in many biological events. But noninvasive characterization of this process in real time at molecular resolution remains challenging. Herein, using homebuilt liquid-phase scanning tunneling microscopy (L-STM) with ultrahigh stability, we directly visualized enzymatic self-assembly/disassembly of oligopeptide nanofibers in real time for the first time. Static high-resolution L-STM images clearly showed the molecular packing details in the supramolecular nanofiber and the diameter of the nanofiber was consistent with that of cryo transmission electron microscopy (cryo-TEM) observations. Moreover, the self-repairing behavior of the supramolecular nanofibers was also directly observed at high resolution for the first time. This work unprecedentedly revealed new insights into Nature-mimic self-assembly and disassembly at the molecular level. It also illustrates the potential of our homebuilt L-STM in studying delicate biological processes in physiological solution with high resolution.
authors: High Magnetic Field Laboratory, Hefei Science Center, Chinese Academy of Sciences and University of Science and Technology of China, Hefei, Anhui, China. junfeng@hmfl.ac.cn qxl@ustc.edu.cn gliang@ustc.edu.cn and CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors: Zhen
authors: Z
authors: Zheng
authors: High Magnetic Field Laboratory, Hefei Science Center, Chinese Academy of Sciences and University of Science and Technology of China, Hefei, Anhui, China. junfeng@hmfl.ac.cn qxl@ustc.edu.cn gliang@ustc.edu.cn and Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors: Jihao
authors: J
authors: Wang
authors: High Magnetic Field Laboratory, Hefei Science Center, Chinese Academy of Sciences and University of Science and Technology of China, Hefei, Anhui, China. junfeng@hmfl.ac.cn qxl@ustc.edu.cn gliang@ustc.edu.cn and CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors: Peiyao
authors: P
authors: Chen
authors: Department of Chemical Physics, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors: Maolin
authors: M
authors: Xie
authors: High Magnetic Field Laboratory, Hefei Science Center, Chinese Academy of Sciences and University of Science and Technology of China, Hefei, Anhui, China. junfeng@hmfl.ac.cn qxl@ustc.edu.cn gliang@ustc.edu.cn.
authors: Lei
authors: L
authors: Zhang
authors: High Magnetic Field Laboratory, Hefei Science Center, Chinese Academy of Sciences and University of Science and Technology of China, Hefei, Anhui, China. junfeng@hmfl.ac.cn qxl@ustc.edu.cn gliang@ustc.edu.cn.
authors: Yubin
authors: Y
authors: Hou
authors: High Magnetic Field Laboratory, Hefei Science Center, Chinese Academy of Sciences and University of Science and Technology of China, Hefei, Anhui, China. junfeng@hmfl.ac.cn qxl@ustc.edu.cn gliang@ustc.edu.cn.
authors: Xin
authors: X
authors: Zhang
authors: Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui 230026, China and Department of Chemical Physics, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors: Jun
authors: J
authors: Jiang
authors: High Magnetic Field Laboratory, Hefei Science Center, Chinese Academy of Sciences and University of Science and Technology of China, Hefei, Anhui, China. junfeng@hmfl.ac.cn qxl@ustc.edu.cn gliang@ustc.edu.cn.
authors: Junfeng
authors: J
authors: Wang
authors: High Magnetic Field Laboratory, Hefei Science Center, Chinese Academy of Sciences and University of Science and Technology of China, Hefei, Anhui, China. junfeng@hmfl.ac.cn qxl@ustc.edu.cn gliang@ustc.edu.cn and CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China and Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing, Jiangsu 210093, China.
authors: Qingyou
authors: Q
authors: Lu
authors: High Magnetic Field Laboratory, Hefei Science Center, Chinese Academy of Sciences and University of Science and Technology of China, Hefei, Anhui, China. junfeng@hmfl.ac.cn qxl@ustc.edu.cn gliang@ustc.edu.cn and CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors: Gaolin
authors: G
authors: Liang