title: Exploiting multi-function Metal-Organic Framework nanocomposite Ag@Zn-TSA as highly efficient immobilization matrixes for sensitive electrochemical biosensing.
abstract: A novel multi-function Metal-Organic Framework composite Ag@Zn-TSA (zinc thiosalicylate, Zn(C7H4O2S), Zn-TSA) was synthesized as highly efficient immobilization matrixes of myoglobin (Mb)/glucose oxidase (GOx) for electrochemical biosensing. The electrochemical biosensors based on Ag@Zn-TSA composite and ionic liquid (IL) modified carbon paste electrode (CPE) were fabricated successfully. Furthermore, the properties of the sensors were discussed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and amperometric current-time curve, respectively. The results showed the proposed biosensors had wide linear response to hydrogen peroxide (H2O2) in the range of 0.3-20,000 μM, to nitrite (NO2(-)) for 1.3 μM-1660 μM and 2262 μM-1,33,000 μM, to glucose for 2.0-1022 μM, with a low detection limit of 0.08 μM for H2O2, 0.5 μM for NO2(-), 0.8 μM for glucose. The values of the apparent heterogeneous electron transfer rate constant (ks) for Mb and GOx were estimated as 2.05 s(-1) and 2.45 s(-1), respectively. Thus, Ag@Zn-TSA was a kind of ideal material as highly efficient immobilization matrixes for sensitive electrochemical biosensing. In addition, this work indicated that MOF nanocomposite had a great potential for constructing wide range of sensing interface.
authors: College of Sciences, Xi'an University of Architecture and Technology, Xi'an, 710055, China. Electronic address: dongsyy@126.com.
authors: Sheying
authors: S
authors: Dong
authors: College of Sciences, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
authors: Dandan
authors: D
authors: Zhang
authors: College of Sciences, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
authors: Gaochao
authors: G
authors: Suo
authors: College of Sciences, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
authors: Wenbo
authors: W
authors: Wei
authors: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
authors: Tinglin
authors: T
authors: Huang
mesh_terms: D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004566:Electrodes; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis; D052578:Ionic Liquids / Q000737:chemistry; D000073396:Metal-Organic Frameworks / Q000737:chemistry*; D015394:Molecular Structure; D009211:Myoglobin / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D009573:Nitrites / Q000032:analysis; D012459:Salicylates / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
chemical_list: D052578:Ionic Liquids; D000073396:Metal-Organic Frameworks; D009211:Myoglobin; D009573:Nitrites; D012459:Salicylates; D013438:Sulfhydryl Compounds; D012834:Silver; D002244:Carbon; D006861:Hydrogen Peroxide; C441201:thiosalicylic acid; D005949:Glucose Oxidase; D005947:Glucose; D015032:Zinc
keywords: Ag@Zn-TSA; Biosensor; Ionic liquid; Metal-Organic Framework; Myoglobin/glucose oxidase