title: Reducible Polyethylenimine Nanoparticles for Efficient siRNA Delivery in Corneal Neovascularization Therapy.
abstract: The aim of this study is to establish the safe and effective ocular delivery system of therapeutic small interfering RNA (siRNA) in corneal neovascularization therapy. The major hurdle present in siRNA-based corneal neovascularization (CNV) therapy is severe cytotoxicity caused by repetitive drug treatment. A reducible branched polyethylenimine (rBPEI)-based nanoparticle (NP) system is utilized as a new siRNA carrier as a hope for CNV therapy. The thiolated BPEI is readily self-crosslinked in mild conditions to make high molecular weight rBPEI thus allowing the creation of stable siRNA/rBPEI nanoparticles (siRNA-rBPEI-NPs). In the therapeutic region, the rBPEI polymeric matrix is effectively degraded into nontoxic LMW BPEI inside the reductive cytosol causing the rapid release of the encapsulated siRNA into the cytosol to carry out its function. The fluorescent-labeled siRNA-rBPEI-NPs can release siRNA into the entire corneal region after subconjuctival injection into the eye of Sprague Dawley rats thus confirming the proof of concept of this system.
authors: Center for Theragnosis, Biomedical Research Institute Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 6, Seongbuk-gu, Seoul, 136-791, South Korea.|Department of Chemical and Biomolecular Engineering, Sogang University, Shinsu-dong, Mapo-gu, Seoul, 121-742, South Korea.
authors: Hyounkoo
authors: H
authors: Han
authors: Center for Theragnosis, Biomedical Research Institute Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 6, Seongbuk-gu, Seoul, 136-791, South Korea.
authors: Sohee
authors: S
authors: Son
authors: Center for Theragnosis, Biomedical Research Institute Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 6, Seongbuk-gu, Seoul, 136-791, South Korea.
authors: Sejin
authors: S
authors: Son
authors: Center for Theragnosis, Biomedical Research Institute Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 6, Seongbuk-gu, Seoul, 136-791, South Korea.
authors: Namho
authors: N
authors: Kim
authors: Center for Theragnosis, Biomedical Research Institute Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 6, Seongbuk-gu, Seoul, 136-791, South Korea.
authors: Ji Young
authors: JY
authors: Yhee
authors: Center for Theragnosis, Biomedical Research Institute Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 6, Seongbuk-gu, Seoul, 136-791, South Korea.|Department of Chemical and Biomolecular Engineering, Sogang University, Shinsu-dong, Mapo-gu, Seoul, 121-742, South Korea.
authors: Jae Hyeop
authors: JH
authors: Lee
authors: Department of Ophthalmology and Visual Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 505, Banpo-dong, Seocho-gu, Seoul, 137-040, South Korea.
authors: Jun-Sub
authors: JS
authors: Choi
authors: Department of Ophthalmology and Visual Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 505, Banpo-dong, Seocho-gu, Seoul, 137-040, South Korea.
authors: Choun-Ki
authors: CK
authors: Joo
authors: Department of Chemical and Biomolecular Engineering, Sogang University, Shinsu-dong, Mapo-gu, Seoul, 121-742, South Korea.
authors: Hohyeon
authors: H
authors: Lee
authors: Center for Self-Assembly and Complexity, Institute for Basic Science (IBS), Pohang, 790-784, South Korea.|Department of Chemistry Polymer Research Institute, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.
authors: Duhwan
authors: D
authors: Lee
authors: Center for Self-Assembly and Complexity, Institute for Basic Science (IBS), Pohang, 790-784, South Korea.|Department of Chemistry Polymer Research Institute, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.
authors: Won Jong
authors: WJ
authors: Kim
authors: Center for Theragnosis, Biomedical Research Institute Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 6, Seongbuk-gu, Seoul, 136-791, South Korea.
authors: Sun Hwa
authors: SH
authors: Kim
authors: Center for Theragnosis, Biomedical Research Institute Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 6, Seongbuk-gu, Seoul, 136-791, South Korea.
authors: Ick Chan
authors: IC
authors: Kwon
authors: Department of Chemical and Biomolecular Engineering, Sogang University, Shinsu-dong, Mapo-gu, Seoul, 121-742, South Korea.
authors: Hyuncheol
authors: H
authors: Kim
authors: Center for Theragnosis, Biomedical Research Institute Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 6, Seongbuk-gu, Seoul, 136-791, South Korea.
authors: Kwangmeyung
authors: K
authors: Kim
mesh_terms: D000818:Animals; D016510:Corneal Neovascularization / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D016503:Drug Delivery Systems / Q000379:methods*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use; D011094:Polyethyleneimine / Q000737:chemistry / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D034741:RNA, Small Interfering; D011094:Polyethyleneimine
keywords: corneal neovascularization; nanoparticles; ocular delivery; reducible polyethylenimine; siRNA