title: 3,3',5,5'-Tetramethylbenzidine Oxidation on Paper Devices for Horseradish Peroxidase-based Assays.
abstract: We report on the colorimetric oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide using horseradish peroxidase on photolithography-fabricated (P-PAD) and wax-printed (W-PAD) paper-based analytical devices. Fabricating PADs via photolithography exposes the hydrophilic areas to polymers (photoresists) and solvents, not only reducing the hydrophilicity, but also affecting the TMB-H2O2 assay system with an unavoidable incomplete elimination of photoresist during fabrication. Detection signals are then observed in the presence of photoresist residues on the P-PAD, even at a blank HRP concentration.
authors: Graduate School of Chemical Sciences and Engineering, Hokkaido University.
authors: Lori Shayne Alamo
authors: LS
authors: Busa
authors: Takeshi
authors: T
authors: Komatsu
authors: Saeed
authors: S
authors: Mohammadi
authors: Masatoshi
authors: M
authors: Maeki
authors: Akihiko
authors: A
authors: Ishida
authors: Hirofumi
authors: H
authors: Tani
authors: Manabu
authors: M
authors: Tokeshi
mesh_terms: D001560:Benzidines / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods; D006735:Horseradish Peroxidase / Q000032:analysis*; D056656:Lab-On-A-Chip Devices; D010084:Oxidation-Reduction; D010209:Paper
chemical_list: D001560:Benzidines; C021758:3,3',5,5'-tetramethylbenzidine; D006735:Horseradish Peroxidase