title: Helper-Dependent Adenoviral Vectors and Their Use for Neuroscience Applications.
abstract: Neuroscience research has been revolutionized by the use of recombinant viral vector technology from the basic, preclinical and clinical levels. Currently, multiple recombinant viral vector types are employed with each having its strengths and weaknesses depending on the proposed application. Helper-dependent adenoviral vectors (HdAd) are emerging as ideal viral vectors that solve a major need in the neuroscience field: (1) expression of transgenes that are too large to be packaged by other viral vectors and (2) rapid onset of transgene expression in the absence of cytotoxicity. Here, we describe the methods for large-scale production of HdAd viral vectors for in vivo use with neurospecific transgene expression.
authors: Research Group Molecular Mechanisms of Synaptic Function, Max Planck Florida Institute for Neuroscience, Jupiter, FL, 33458, USA.
authors: Mónica S
authors: MS
authors: Montesinos
authors: Research Group Molecular Mechanisms of Synaptic Function, Max Planck Florida Institute for Neuroscience, Jupiter, FL, 33458, USA.
authors: Rachel
authors: R
authors: Satterfield
authors: Research Group Molecular Mechanisms of Synaptic Function, Max Planck Florida Institute for Neuroscience, Jupiter, FL, 33458, USA. Sam.Young@mpfi.org.
authors: Samuel M
authors: SM
authors: Young
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism*; D057809:HEK293 Cells; D006378:Helper Viruses / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009488:Neurosciences / Q000379:methods*; D019076:Transgenes
keywords: Adevnovirus; Cell-type specific; Neuroscience; Recombinant viral vectors; Reporters; Transgene expression