title: Thymine DNA Glycosylase Gene Knockdown Can Affect the Differentiation of Pig Preadipocytes.
abstract: OBJECTIVE
  To study the effect of thymine DNA glycosylase (TDG) gene knockdown on the differentiation of pig preadipocytes.
  
  
  METHODS
  Preadipocytes were obtained from subcutaneous adipose tissue from the neck of 1- to 7-day-old pigs. The TDG gene was knocked down using siRNA, and cell differentiation was induced. The mRNA expression level was measured using fluorescence quantitative PCR, and the protein expression level was determined using Western blot analysis. The DNA methylation levels in promoter regions of differentiation-related genes were also evaluated.
  
  
  RESULTS
  TDG gene knockdown decreased the mRNA expression levels of the peroxisome proliferator-activated receptorγ (PPARγ) and Fatty acid binding proteins 4(FABP4 Also known as aP2) genes (P<0.01), while the mRNA expression level of the CCAAT/enhancer binding protein alpha(C/EBPα) gene did not change significantly (P>0.05). In addition, after induced differentiation, the lipid droplet production significantly decreased, and the percentages of methylation in the promoter regions of C/EBPα, PPARγ, and aP2 genes were 0.9%, 80%, and 76%, respectively. In contrast, the percentages of methylation in the negative control groups were 0.5%, 67.5%, and 58%, respectively.
  
  
  CONCLUSIONS
  TDG gene knockdown could inhibit the differentiation of pig preadipocytes and affect the DNA methylation levels of some transcription factors.
authors: College of Animal Science and Technology, Jilin Agricultural Science and Technology College, Jilin, China.
authors: Lian-Jiang
authors: LJ
authors: Zhang
authors: Si-Yuan
authors: SY
authors: Liu
authors: Ya-Nan
authors: YN
authors: Zhu
authors: Yan
authors: Y
authors: Gao
authors: Jian
authors: J
authors: Chen
authors: Bao
authors: B
authors: Yuan
authors: Hao
authors: H
authors: Jiang
authors: Li-Sheng
authors: LS
authors: Dai
authors: Jia-Bao
authors: JB
authors: Zhang
mesh_terms: D052438:Adipocytes, White / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D066292:Lipid Droplets / Q000378:metabolism; D008297:Male; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism; D061251:Primary Cell Culture; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D050151:Subcutaneous Fat / Q000166:cytology / Q000378:metabolism; D013552:Swine; D044502:Thymine DNA Glycosylase / Q000172:deficiency / Q000235:genetics*
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D050556:Fatty Acid-Binding Proteins; D047495:PPAR gamma; D012333:RNA, Messenger; D044502:Thymine DNA Glycosylase