title: Characterization of two LuxI/R homologs in Pantoea ananatis LMG 2665T.
abstract: Quorum sensing (QS) plays an important role in the regulation of bacteria-host interactions and ecological fitness in many bacteria. In this study, 2 luxI/R homologs, namely eanI/eanR and rhlI/rhlR, were identified in the genome sequence of Pantoea ananatis LMG 2665T. To determine a role for these luxI/R homologs in pathogenicity and biofilm formation, mutant bacterial strains lacking either eanI/R or rhlI/R and both of these homologs were generated. The results indicated that both the RhlI/R and EanI/R systems are required for pathogenicity and biofilm formation in strain LMG 2665T. This is the first study to characterize the biological significance of the RhlI/R QS system in P. ananatis.
authors: a Department of Microbiology and Plant Pathology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, Republic of South Africa.|b Forestry and Agricultural Biotechnology Institute, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, Republic of South Africa.
authors: Siphathele
authors: S
authors: Sibanda
authors: a Department of Microbiology and Plant Pathology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, Republic of South Africa.
authors: Jacques
authors: J
authors: Theron
authors: a Department of Microbiology and Plant Pathology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, Republic of South Africa.
authors: Divine Y
authors: DY
authors: Shyntum
authors: a Department of Microbiology and Plant Pathology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, Republic of South Africa.|b Forestry and Agricultural Biotechnology Institute, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, Republic of South Africa.
authors: Lucy N
authors: LN
authors: Moleleki
authors: a Department of Microbiology and Plant Pathology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, Republic of South Africa.|b Forestry and Agricultural Biotechnology Institute, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, Republic of South Africa.
authors: Teresa A
authors: TA
authors: Coutinho
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms; D016680:Genome, Bacterial / Q000235:genetics; D009154:Mutation / Q000235:genetics; D020636:Pantoea / Q000235:genetics* / Q000472:pathogenicity*; D053038:Quorum Sensing / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C085427:LuxI protein, Bacteria; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C064778:LuxR autoinducer binding proteins
keywords: LuxI/R; Pantoea ananatis; détection du quorum; pathogenicity; pathogénicité; quorum sensing