title: Salvianolic acid B protects against paraquat-induced pulmonary injury by mediating Nrf2/Nox4 redox balance and TGF-β1/Smad3 signaling.
abstract: The present study was aimed at exploring the protective effects of Salvianolic acid B (SalB) against paraquat (PQ)-induced lung injury in mice. Lung fibrotic injuries were induced in mice by a single intragastrical administration of 300mg/kg PQ, then the mice were administrated with 200mg/kg, 400mg/kg SalB, 100mg/kg vitamin C (Vit C) and dexamethasone (DXM) for 14days. PQ-triggered structure distortion, collagen overproduction, excessive inflammatory infiltration, pro-inflammatory cytokine release, and oxidative stress damages in lung tissues and mortality of mice were attenuated by SalB in a dose-dependent manner. Furthermore, SalB was noted to enhance the expression and nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and reduce expression of the reactive oxygen species-generating enzyme Nox4 [NADPH (reduced form of nicotinamide adenine dinucleotide phosphate) oxidase-4]. SalB also inhibited the increasing expression of transforming growth factor (TGF)-β1 and the phosphorylation of its downstream target Smad3 which were enhanced by PQ. These results suggest that SalB may exert protective effects against PQ-induced lung injury and pulmonary fibrosis. Its mechanisms involve the mediation of Nrf2/Nox4 redox balance and TGF-β1/Smad3 signaling.
authors: Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Logistic University of Chinese People's Armed Police Force, Tianjin 300162, China. Electronic address: iamicehe@163.com.
authors: Bin
authors: B
authors: Liu
authors: Logistics University of Chinese People's Armed Police Force, Tianjin 300162, China; Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Tianjin, 300162, China. Electronic address: caobo19814@126.com.
authors: Bo
authors: B
authors: Cao
authors: Department of Otorhinolaryngology Head and Neck Surgery, Institute of Otorhinolaryngology, Tianjin First Center Hospital, Tianjin 300192, China. Electronic address: zhangdibad@163.com.
authors: Di
authors: D
authors: Zhang
authors: Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Logistic University of Chinese People's Armed Police Force, Tianjin 300162, China.
authors: Na
authors: N
authors: Xiao
authors: Logistics University of Chinese People's Armed Police Force, Tianjin 300162, China.
authors: Hong
authors: H
authors: Chen
authors: Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Logistic University of Chinese People's Armed Police Force, Tianjin 300162, China.
authors: Guo-Qiang
authors: GQ
authors: Li
authors: Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Logistic University of Chinese People's Armed Police Force, Tianjin 300162, China.
authors: Shou-Chun
authors: SC
authors: Peng
authors: Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Logistic University of Chinese People's Armed Police Force, Tianjin 300162, China. Electronic address: luqing-wei@163.com.
authors: Lu-Qing
authors: LQ
authors: Wei
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D006540:Herbicides / Q000633:toxicity*; D018836:Inflammation Mediators / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases / Q000378:metabolism*; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010269:Paraquat / Q000633:toxicity*; D051900:Smad3 Protein / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D053630:Depsides
chemical_list: D001572:Benzofurans; D006540:Herbicides; D018836:Inflammation Mediators; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases; D051267:NF-E2-Related Factor 2; D010269:Paraquat; D051900:Smad3 Protein; D053773:Transforming Growth Factor beta1; C495636:Nfe2l2 protein, mouse; C497791:Smad3 protein, mouse; C076944:salvianolic acid B; D053630:Depsides; C422943:Nox4 protein, mouse
keywords: NOX4; Nrf2; Paraquat; Pulmonary fibrosis; Salvianolic acid B; TGF-β1/Smad3 signaling pathway