title: Strontium-doped organic-inorganic hybrids towards three-dimensional scaffolds for osteogenic cells.
abstract: Biomimetic organic-inorganic hybrid bioscaffolds are developed to complement or replace damaged fragments in bone tissue surgery. The aim of this work was to develop a simple and fast method to prepare composite material for bone engineering, avoiding time consuming and complex methodologies. The resulting materials (also called in this work as hybrid composites or hybrid scaffolds) have a three-dimensional macroporous polymer-like network derived from triethoxyvinylsilane (TEVS) and 2-hydroxyethylmethacrylate (HEMA) monomers, with incorporated calcium, strontium, and phosphate ions. The materials were fully characterized using FT-IR, biomineralization studies, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy, scratch tests, Young's modulus and compressive strength tests, and gas physisorption. We report a comprehensive study on the in vitro effect of novel strontium doped materials on human bone cells. In vitro investigations were conducted using a normal human osteoblast cell line that mimics the cellular events of the in vivo intramembranous bone formation process. The materials do not have a negative impact on the survival of the normal human osteoblasts; moreover, materials doped with strontium show that not only are cells able to survive, but they also attach to and grow on a bioscaffolds surface. For this reason, they may be used in future in vivo experiments.
authors: Faculty of Chemistry, University of Wrocław, 14 F. Joliot-Curie, 50-383 Wrocław, Poland. Electronic address: lukasz.john@chem.uni.wroc.pl.
authors: Łukasz
authors: Ł
authors: John
authors: Faculty of Chemistry, University of Wrocław, 14 F. Joliot-Curie, 50-383 Wrocław, Poland.
authors: Marta
authors: M
authors: Podgórska
authors: Université Clermont Auvergne, SIGMA Clermont, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63178 Aubiere, France.
authors: Jean-Marie
authors: JM
authors: Nedelec
authors: Department of Histology and Embryology, Wrocław Medical University, 6a Chałubińskiego, 50-368 Wrocław, Poland.
authors: Łucja
authors: Ł
authors: Cwynar-Zając
authors: Department of Histology and Embryology, Wrocław Medical University, 6a Chałubińskiego, 50-368 Wrocław, Poland; Department of Physiotherapy and Occupational Therapy in Conservative and Interventional Medicine, 35 Paderewskiego, 51-612 Wrocław, Poland.
authors: Piotr
authors: P
authors: Dzięgiel
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects*; D012821:Silanes / Q000737:chemistry / Q000494:pharmacology; D013324:Strontium / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D012821:Silanes; C005044:hydroxyethyl methacrylate; C052671:vinyltriethoxysilane; D013324:Strontium
keywords: Bone; Organic–inorganic hybrids; Osteoblasts; Osteoporosis; Scaffolds; Strontium