title: Tobacco as platform for a commercial production of cyanophycin.
abstract: Cyanophycin (CP) is a proteinogenic polymer that can be substituted for petroleum in the production of plastic compounds and can also serve as a source of valuable dietary supplements. However, because there is no economically feasible system for large-scale industrial production, its application is limited. In order to develop a low-input system, CP-synthesis was established in the two commercial Nicotiana tabacum (N. tabacum) cultivars 'Badischer Geudertheimer' (BG) and 'Virginia Golta' (VG), by introducing the cyanophycin-synthetase gene from Thermosynecchococcus elongatus BP-1 (CphATe) either via crossbreeding with transgenic N. tabacum cv. Petit Havana SR1 (PH) T2 individual 51-3-2 or by agrobacterium-mediated transformation. Both in F1 hybrids (max. 9.4% CP/DW) and T0 transformants (max. 8.8% CP/DW), a substantial increase in CP content was achieved in leaf tissue, compared to a maximum of 1.7% CP/DW in PH T0 transformants of Hühns et al. (2008). In BG CP, yields were homogenous and there was no substantial difference in the variation of the CP content between primary transformants (T0), clones of T0 individuals, T1 siblings and F1 siblings of hybrids. Therefore, BG meets the requirements for establishing a master seed bank for continuous and reliable CP-production. In addition, it was shown that the polymer is not only stable in planta but also during silage, which simplifies storage of the harvest prior to isolation of CP.
authors: University of Rostock, Faculty of Agricultural and Environmental Sciences, Department of Agrobiotechnology and Risk Assessment for Bio- und Gene Technology, Justus-von-Liebig Weg 8, Mecklenburg-Western Pomerania, 18059, Rostock, Germany. Electronic address: henrik.nausch@uni-rostock.de.
authors: Henrik
authors: H
authors: Nausch
authors: University of Rostock, Faculty of Agricultural and Environmental Sciences, Department of Agrobiotechnology and Risk Assessment for Bio- und Gene Technology, Justus-von-Liebig Weg 8, Mecklenburg-Western Pomerania, 18059, Rostock, Germany.
authors: Tina
authors: T
authors: Hausmann
authors: University of Rostock, Faculty of Agricultural and Environmental Sciences, Department of Agrobiotechnology and Risk Assessment for Bio- und Gene Technology, Justus-von-Liebig Weg 8, Mecklenburg-Western Pomerania, 18059, Rostock, Germany.
authors: Daniel
authors: D
authors: Ponndorf
authors: University of Rostock, Faculty of Agricultural and Environmental Sciences, Department of Agrobiotechnology and Risk Assessment for Bio- und Gene Technology, Justus-von-Liebig Weg 8, Mecklenburg-Western Pomerania, 18059, Rostock, Germany.
authors: Maja
authors: M
authors: Hühns
authors: University of Rostock, Faculty of Agricultural and Environmental Sciences, Department of Nutrition Physiology and Animal Nutrition, Justus-von-Liebig-Weg 6b, Mecklenburg-Western Pomerania, 18059, Rostock, Germany.
authors: Sandra
authors: S
authors: Hoedtke
authors: University of Rostock, Faculty of Agricultural and Environmental Sciences, Department of Nutrition Physiology and Animal Nutrition, Justus-von-Liebig-Weg 6b, Mecklenburg-Western Pomerania, 18059, Rostock, Germany.
authors: Petra
authors: P
authors: Wolf
authors: Martin-Luther-University Halle-Wittenberg, Institute for Agricultural and Nutritional Sciences, Chair of Animal Nutrition, Theodor-Lieser-Str. 11, 06120, Halle (Saale), Germany.
authors: Annette
authors: A
authors: Zeyner
authors: University of Rostock, Faculty of Agricultural and Environmental Sciences, Department of Agrobiotechnology and Risk Assessment for Bio- und Gene Technology, Justus-von-Liebig Weg 8, Mecklenburg-Western Pomerania, 18059, Rostock, Germany.
authors: Inge
authors: I
authors: Broer
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D018533:Biomass; D001709:Biotechnology; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics; D005285:Fermentation; D006824:Hybridization, Genetic; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D055550:Protein Stability; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D014170:Transformation, Genetic
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; C024250:cyanophycin; D010453:Peptide Synthases; C113257:cyanophycin synthase, bacteria
keywords: Badischer Geudertheimer; Commercial production; Cyanophycin; Nicotiana tabacum cv. Petit Havana SR1; Virginia Golta