title: [Effect and mechanism of interleukin-8 expression induced by wood smoke particles in primary human airway epithelial cells].
abstract: OBJECTIVE
  To investigate the effect and mechanism of interleukin-8 expression induced by wood smoke particles (WSP) in primary human airway epithelial cells.
  
  
  METHODS
  Primary human bronchial epithelial cells (HBEC) were collected through fiberbronchoscopic brushing and incubated with different concentration of WSP (0, 12.5, 25.0, 50.0, 100.0 μg/ml). Levels of IL-8 protein in cell culture supernatant were measured using enzyme-linked immune sorbent assay. The specific inhibitors for epidermal growth factor receptor (EGFR) and p38 kinase signaling pathways were employed to pretreat HBEC, respectively, prior to incubation with 100 μg/ml WSP to investigate the mechanism of IL-8 expression induced by WSP.
  
  
  RESULTS
  The expression of IL-8 was significant increased in a dose-dependent manner after exposure HBEC to different concentration of WSP for 24h. The levels of IL-8 expression were (4546.67±1421.42) ρg/ml in (PD153035+WSP) group and (2803.33±865.00) ρg/ml in (SB203580+WSP) group respectively, which were significant decreased compared with the level of (12896.67±1373.11) ρg/ml in WSP group (P<0.05).
  
  
  CONCLUSIONS
  WSP could induce IL-8 expression by means of EGFR and p38 signaling pathways.
authors: Department of Occupational Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
authors: Y J
authors: YJ
authors: Yan
authors: W D
authors: WD
authors: Wu
authors: W J
authors: WJ
authors: Liu
authors: Y Y
authors: YY
authors: Liu
authors: X
authors: X
authors: Liang
authors: H B
authors: HB
authors: Li
authors: W
authors: W
authors: Yao
authors: Z
authors: Z
authors: Yan
mesh_terms: D002478:Cells, Cultured; D004847:Epithelial Cells; D066246:ErbB Receptors; D006801:Humans; D016209:Interleukin-8; D020935:MAP Kinase Signaling System; D012906:Smoke; D014934:Wood
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8; D012906:Smoke; C512478:EGFR protein, human; D066246:ErbB Receptors