title: The protective mechanism of quercetin-3-O-β-D-glucuronopyranoside (QGC) in H2O2-induced injury of feline esophageal epithelial cells.
abstract: Quercetin-3-O-β-D-glucuronopyranoside (QGC) is a flavonoid glucoside extracted from Rumex Aquaticus. Recent studies have shown that QGC exhibits anti-inflammatory, anti-oxidateve effect in vivo and cytoprotective effect in vitro. Reactive oxygen species (ROS), at low concentration, play role as a primary signal or second messenger, however, at high concentration, ROS are cytotoxic. In this study, we investigated the protective mechanism of QGC in H2O2-induced injury of Feline Esophageal Epithelial Cells. Primary-cultured feline esophagus cells were identified by an indirect immunofluorescent staining method using a cytokeratin monoclonal antibody. Cell viability was determined by the conventional MTT reduction assay. Western blot analysis was performed with specific antibodies to investigate the activation of MAPKs, NF-κB, and IκB-α, and the expression of COX-2. When the cells were exposed to 600 μM H2O2 medium for 24 h, cell viability decreased to 54 %. However, when cells were pretreated with 50-150 μM QGC for 12 h, the viability of cells exposed to H2O2 significantly increased in the dose dependent manner. QGC (50 μM, 12 h) also inhibited the expression of COX-2 induced by 10 μM H2O2 for 24 h. We found that treatment of H2O2 activated p38 MAPK and JNK, but not ERK. However QGC inhibited the H2O2-induced p38 MAPK and JNK phosphorylation. In addition, NF-κB was activated by H2O2 and translocated into the nucleus, but QGC inhibited the activation of NF-κB by blocking degradation of IκB. These data suggest that QGC reduces H2O2-induced COX-2 production by modulating the p38 MAPK, JNK, NF-κB signal pathway in feline esophageal epithelial cells.
authors: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors: Uy Soo
authors: US
authors: Sohn
authors: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors: Se Eun
authors: SE
authors: Lee
authors: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors: Sung Hee
authors: SH
authors: Lee
authors: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors: Yoonjin
authors: Y
authors: Nam
authors: Department of Pharmaceutical botany, College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors: Wan Kyunn
authors: WK
authors: Hwang
authors: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea. udsohn@cau.ac.kr.
authors: Uy Dong
authors: http://orcid.org/0000-0002-1801-7551
authors: UD
authors: Sohn
mesh_terms: D000818:Animals; D002415:Cats; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D000071041:Esophageal Mucosa / Q000187:drug effects* / Q000293:injuries* / Q000378:metabolism; D005260:Female; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008297:Male; D009924:Organ Culture Techniques; D020011:Protective Agents / Q000494:pharmacology; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D020011:Protective Agents; C443401:quercetin 3-O-glucuronide; D011794:Quercetin; D006861:Hydrogen Peroxide
keywords: Anti-oxidative; Feline esophageal epithelial cells; Inflammation; Mitogen-activated protein kinase (MAPK); Quercetin-3-O-β-D-glucuronopyranoside (QGC); Reactive oxygen speicies (ROS)