title: FACS Fractionation and Differentiation of Skeletal-Muscle Resident Multipotent Tie2+ Progenitors.
abstract: The skeletal muscle niche is complex and heterogeneous. Over the past few decades, various groups have reported the existence of multiple adult stem cell populations within this environment. Techniques commonly used to identify and assess the differentiation capacities of these cellular fractions, oftentimes rare populations, include the use of lineage tracers, immunofluorescence and histochemistry, flow cytometry, gene expression assays, and phenotypic analysis in culture or in vivo. In 2012, our lab identified and characterized a skeletal-muscle resident Tie2+ progenitor that exhibits adipogenic, chondrogenic, and osteogenic differentiation potentials (Wosczyna et al., J Bone Miner Res 27:1004-1017, 2012). This Tie2+ progenitor also expresses the markers PDGFRα and Sca-1 which in turn label a population of muscle-resident fibro/adipogenic progenitors (FAPs) (Joe et al., Nat Cell Biol 12:153-163, 2010; Uezumi et al., Nat Cell Biol 12:143-152, 2010), suggesting similar identities or overlap in the two mesenchymal progenitor populations. Our study demonstrated that these Tie2-expressing mesenchymal progenitors contribute robustly to BMP-induced heterotopic ossification (HO) in mice, and therefore could represent a key cellular target for therapeutic intervention in HO treatment (Wosczyna et al., J Bone Miner Res 27:1004-1017, 2012). In this chapter, we provide a detailed description of our updated fluorescence-activated cell sorting (FACS) strategy and describe cell culture methods for differentiation of Tie2+ progenitors to adipogenic and osteogenic fates. This strategy is easily adaptable for the prospective isolation of other rare subpopulations resident in skeletal muscle.
authors: Department of Molecular & Cell Biology, University of Connecticut Stem Cell Institute, University of Connecticut, 91 N. Eagleville Road, Unit 3125, Storrs, CT, 06269, USA.
authors: Arpita A
authors: AA
authors: Biswas
authors: Department of Molecular & Cell Biology, University of Connecticut Stem Cell Institute, University of Connecticut, 91 N. Eagleville Road, Unit 3125, Storrs, CT, 06269, USA. david.goldhamer@uconn.edu.
authors: David J
authors: DJ
authors: Goldhamer
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D005434:Flow Cytometry / Q000379:methods; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000166:cytology*; D010012:Osteogenesis; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism; D042787:Receptor, TIE-2 / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D000950:Antigens, Ly; D015415:Biomarkers; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D020796:Receptor, Platelet-Derived Growth Factor alpha; D042787:Receptor, TIE-2; C079086:Tek protein, mouse
keywords: Adipogenesis; Cre; Fibro/adipogenic progenitors; Flow cytometry; Heterotopic ossification; Osteogenesis; PDGFRα; Sca-1; Skeletal muscle stem cell; Tie2