title: Angiostrongylus cantonensis and A. malaysiensis Broadly Overlap in Thailand, Lao PDR, Cambodia and Myanmar: A Molecular Survey of Larvae in Land Snails.
abstract: Angiostrongylus cantonensis is a zoonotic nematode parasite causing human eosinophilic meningitis (or meningoencephalitis) worldwide. A closely related species, Angiostrongylus malaysiensis, might also be a human pathogen. Larvae were obtained from land snails in Lao PDR, Cambodia, Myanmar and Thailand. We sequenced two nuclear gene regions (nuclear ribosomal ITS2 and SSU rRNA) and a portion of one mitochondrial gene (COI) from these larvae. Angiostrongylus cantonensis and A. malaysiensis were identified. This is the first report of the molecular identification of the two Angiostrongylus species in Lao PDR, Cambodia and Myanmar. The regional distributions of the two species broadly overlap. Phylogenetic relationships were inferred including data from Angiostrongylus species deposited in public databases. All the gene regions we sequenced have potential value in distinguishing between species of Angiostrongylus. The COI gene exhibited the greatest intraspecific variation in the study region (five haplotypes in A. cantonensis and four in A. malaysiensis) and might be suitable for more detailed phylogeographic studies.
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: Rutchanee
authors: R
authors: Rodpai
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: Pewpan M
authors: PM
authors: Intapan
authors: Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand.
authors: Tongjit
authors: T
authors: Thanchomnang
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: Oranuch
authors: O
authors: Sanpool
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: Lakkhana
authors: L
authors: Sadaow
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Laboratory Unit, Centre of Malariology, Parasitology and Entomology, Ministry of Health, Vientiane, Lao People's Democratic Republic.
authors: Sakhone
authors: S
authors: Laymanivong
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Department of Microbiology, University of Medicine 2, Yangon, Myanmar.
authors: Win Papa
authors: WP
authors: Aung
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: Issarapong
authors: I
authors: Phosuk
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: Porntip
authors: P
authors: Laummaunwai
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: Wanchai
authors: http://orcid.org/0000-0002-6726-3759
authors: W
authors: Maleewong
mesh_terms: D017185:Angiostrongylus cantonensis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D003576:Electron Transport Complex IV / Q000235:genetics; D007814:Larva / Q000235:genetics* / Q000469:parasitology; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics*; D012908:Snails / Q000235:genetics* / Q000469:parasitology; D017206:Strongylida Infections / Q000453:epidemiology / Q000469:parasitology*; D013785:Thailand / Q000453:epidemiology
chemical_list: D012335:RNA, Ribosomal; D003576:Electron Transport Complex IV