title: AMPK-dependent regulation of GLP1 expression in L-like cells.
abstract: This study examined whether AMPK, an evolutionarily conserved sensor of cellular energy status, determines the production of glucagon-like peptide-1 (GLP1). A negative relation existed between phosphorylation of AMPKα and the expression and secretion of GLP1 during changes in energy status in STC-1 cells, an L-like cell line. High concentration of glucose (25 mmol/L) decreased AMPKα phosphorylation, whereas it stimulated the expression and secretion of GLP1 relative to 5.6 mmol/L glucose. Serum starvation upregulated AMPKα phosphorylation, whereas it reduced GLP1 production significantly. Stimulation of AMPK phosphorylation by AICAR and overexpression of wild-type AMPKα1, constitutively active AMPKα1 plasmids, or AMPKα1 lentivirus particles suppressed proglucagon mRNA and protein contents in STC-1 cells. Inactivation of AMPK by Compound C, AMPKα1 siRNA or kinase-inactive AMPKα1 mutant increased the expression and secretion of GLP1. Our results suggest that AMPKα1 may link energy supply with the production of GLP1 in L-like cells.
authors: Department of PhysiologySchool of Medicine, Jinan University, Guangzhou, Guangdong, China.
authors: Sushi
authors: S
authors: Jiang
authors: Department of PhysiologySchool of Medicine, Jinan University, Guangzhou, Guangdong, China Endoscopy CenterThe First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China.
authors: Hening
authors: H
authors: Zhai
authors: Department of PhysiologySchool of Medicine, Jinan University, Guangzhou, Guangdong, China.
authors: Danjie
authors: D
authors: Li
authors: Department of PhysiologySchool of Medicine, Jinan University, Guangzhou, Guangdong, China.
authors: Jiana
authors: J
authors: Huang
authors: Department of PhysiologySchool of Medicine, Jinan University, Guangzhou, Guangdong, China.
authors: Heng
authors: H
authors: Zhang
authors: Shenzhen University Diabetes CenterShenzhen University Health Science Center, Shenzhen, Guangdong, China.
authors: Ziru
authors: Z
authors: Li
authors: Endoscopy CenterThe First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China Department of SurgeryUniversity of Michigan Medical Center, Ann Arbor, Michigan, USA weizhenz@umich.edu xugeyangliang@163.com.
authors: Weizhen
authors: W
authors: Zhang
authors: Department of PhysiologySchool of Medicine, Jinan University, Guangzhou, Guangdong, China weizhenz@umich.edu xugeyangliang@163.com.
authors: Geyang
authors: G
authors: Xu
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D001786:Blood Glucose; D002460:Cell Line; D003106:Colon; D005786:Gene Expression Regulation; D052216:Glucagon-Like Peptide 1 / Q000235:genetics* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D010766:Phosphorylation
chemical_list: D001786:Blood Glucose; D052216:Glucagon-Like Peptide 1; D055372:AMP-Activated Protein Kinases; D005947:Glucose
keywords: AMPKα1; GLP1; energy sensing; enteroendocrine L cells