title: Concurrent quantification of multiple biomarkers indicative of oxidative stress status using liquid chromatography-tandem mass spectrometry.
abstract: 8-Hydroxy-2-deoxyguanosine (8-OHdG), 8-nitroguanine (8-NO2Gua), 8-iso-prostaglandin F2α (8-IsoPGF2α), and N-acetyl-S-(tetrahydro-5-hydroxy-2-pentyl-3-furanyl)-L-cysteine (HNE-MA) are well-studied and representative biomarkers for oxidative DNA damage, inflammation, and lipid peroxidation; all of which have been associated with increases in risks of various diseases and cancers. A rapid and highly sensitive isotope-dilution liquid-chromatography tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously quantify the aforementioned biomarkers in urine. Upon validation, this method shows excellent feasibility, sensitivity (0.008-0.03 ng/mL) and satisfactory recoveries (88.7-95.4%); the calibration curves displayed excellent linearity with coefficients of determination (R(2)) greater than 0.998. Additionally, low variations were observed in the relative standard deviation for intra- and inter-day measurements for the four analytes. The relative matrix effects for all four analytes ranged from 2.04 to 3.27%, which signaled that interferences from endogenous levels of the analytes were deemed statistically insignificant. This study successfully developed an analytical method capable to simultaneously quantify urinary 8-OHdG, 8-NO2Gua, 8-IsoPGF2α, and HNE-MA. This analytical protocol can be applied towards conducting epidemiological studies to reveal the mechanisms related to disease development, and thus evaluate the associated risks of diseases.
authors: Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, No. 17, ShiuJou Rd., Taipei 10055, Taiwan.
authors: Charlene
authors: C
authors: Wu
authors: Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, No. 17, ShiuJou Rd., Taipei 10055, Taiwan; National Environmental Health Research Center, National Health Research Institutes, No. 35, Keyan Rd., Zhunan, Miaoli County 35053, Taiwan.
authors: Shu-Ting
authors: ST
authors: Chen
authors: Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, No. 17, ShiuJou Rd., Taipei 10055, Taiwan.
authors: Kui-Han
authors: KH
authors: Peng
authors: Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, No. 17, ShiuJou Rd., Taipei 10055, Taiwan.
authors: Tsun-Jen
authors: TJ
authors: Cheng
authors: Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, No. 17, ShiuJou Rd., Taipei 10055, Taiwan. Electronic address: kuenyuhwu@ntu.edu.tw.
authors: Kuen-Yuh
authors: KY
authors: Wu
mesh_terms: D015415:Biomarkers / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods; D004249:DNA Damage; D005260:Female; D006151:Guanosine / Q000031:analogs & derivatives* / Q000652:urine*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D018384:Oxidative Stress
chemical_list: D015415:Biomarkers; D006151:Guanosine
keywords: Biomarkers; Isotope dilution; Liquid chromatography-tandem mass spectrometry; Oxidative stress; Urine