title: Jasmonate-mediated stomatal closure under elevated CO2revealed by time-resolved metabolomics.
abstract: Foliar stomatal movements are critical for regulating plant water loss and gas exchange. Elevated carbon dioxide (CO2) levels are known to induce stomatal closure. However, the current knowledge on CO2signal transduction in stomatal guard cells is limited. Here we report metabolomic responses of Brassica napus guard cells to elevated CO2using three hyphenated metabolomics platforms: gas chromatography-mass spectrometry (MS); liquid chromatography (LC)-multiple reaction monitoring-MS; and ultra-high-performance LC-quadrupole time-of-flight-MS. A total of 358 metabolites from guard cells were quantified in a time-course response to elevated CO2level. Most metabolites increased under elevated CO2, showing the most significant differences at 10 min. In addition, reactive oxygen species production increased and stomatal aperture decreased with time. Major alterations in flavonoid, organic acid, sugar, fatty acid, phenylpropanoid and amino acid metabolic pathways indicated changes in both primary and specialized metabolic pathways in guard cells. Most interestingly, the jasmonic acid (JA) biosynthesis pathway was significantly altered in the course of elevated CO2treatment. Together with results obtained from JA biosynthesis and signaling mutants as well as CO2signaling mutants, we discovered that CO2-induced stomatal closure is mediated by JA signaling.
authors: Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, 32610, USA.|Department of Biology, Genetics Institute, University of Florida, Gainesville, FL, 32610, USA.
authors: Sisi
authors: S
authors: Geng
authors: Department of Biology, Genetics Institute, University of Florida, Gainesville, FL, 32610, USA.
authors: Biswapriya B
authors: BB
authors: Misra
authors: Thermo Fisher Scientific, 1400 Northpoint Parkway, West Palm Beach, FL, 33407, USA.
authors: Evaldo
authors: E
authors: de Armas
authors: Samuel Roberts Noble Foundation, Ardmore, OK, 73401, USA.
authors: David V
authors: DV
authors: Huhman
authors: Chemistry Research Unit, Agricultural Research Service, United States Department of Agriculture, Gainesville, FL, 32608, USA.
authors: Hans T
authors: HT
authors: Alborn
authors: Samuel Roberts Noble Foundation, Ardmore, OK, 73401, USA.
authors: Lloyd W
authors: LW
authors: Sumner
authors: Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, 32610, USA.|Department of Biology, Genetics Institute, University of Florida, Gainesville, FL, 32610, USA.|Proteomics and Mass Spectrometry, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, 32610, USA.
authors: Sixue
authors: S
authors: Chen
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D029688:Brassica napus / Q000235:genetics / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D055432:Metabolomics / Q000379:methods*; D054883:Oxylipins / Q000378:metabolism*; D054046:Plant Stomata / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D017382:Reactive Oxygen Species; D002245:Carbon Dioxide; C011006:jasmonic acid
keywords: 
  Arabidopsis thaliana
  ; 
  Brassica napus
  ; carbon dioxide; guard cell; jasmonate signaling; metabolomics; reactive oxygen species; stomatal movement