title: A novel approach of integrated bioprocessing of cane molasses for production of prebiotic and functional bioproducts.
abstract: In this work, the sugar industry by-product cane molasses was investigated as feedstock for acceptor reactions by dextransucrase from Leuconostoc mesenteroides MTCC 10508, leading to the biosynthesis of oligosaccharides. The starch industry corn fiber residue was used as a source for acceptor molecules, maltose, in the reaction. Production of approximately 124g oligosaccharides (DP3-DP6) per kg of fresh molasses was achieved. Further, cane molasses based medium was demonstrated as a sole carbon source for L. mesenteroides growth and dextransucrase production. d-Fructose released by dextransucrase activity as processing by-product was transformed into the functional monosaccharide with zero caloric value, d-psicose, by inducing its epimerization. Quantitative analysis approximated 37g d-psicose per kg of fresh molasses. Thus, the study established a novel approach of integrated bioprocessing of cane molasses into prebiotic and functional food additives.
authors: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India.
authors: Manisha
authors: M
authors: Sharma
authors: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India.
authors: Satya Narayan
authors: SN
authors: Patel
authors: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India.
authors: Kusum
authors: K
authors: Lata
authors: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India.
authors: Umesh
authors: U
authors: Singh
authors: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India.
authors: Meena
authors: M
authors: Krishania
authors: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India.
authors: Rajender S
authors: RS
authors: Sangwan
authors: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India. Electronic address: pratapsudhir17@gmail.com.
authors: Sudhir P
authors: SP
authors: Singh
mesh_terms: D005632:Fructose / Q000096:biosynthesis; D006868:Hydrolysis; D007934:Leuconostoc / Q000201:enzymology*; D008965:Molasses; D009844:Oligosaccharides / Q000096:biosynthesis*; D056692:Prebiotics
chemical_list: D009844:Oligosaccharides; D056692:Prebiotics; C003243:psicose; D005632:Fructose
keywords: Cane molasses; Dextransucrase; Leuconostoc mesenteroides; Oligosaccharides; Prebiotics; d-Psicose; d-Psicose 3-epimerase