title: Immunomagnetic separation as a final purification step of liver endothelial cells.
abstract: We describe a fast and reproducible method that can be used as a final step in obtaining pure populations of liver endothelial cells. This method employs endothelial cell specific lectin covalently bound to magnetic polystyrene beads (Dynabeads). Evonymus europaeus agglutinin (EEA)-coated Dynabeads were used to purify monkey liver endothelium from Percoll gradient separated nonparenchymal cells. EEA-coated beads were also successfully used to purify monkey aortic endothelial cells. The endothelial cells grew to confluence as a cobblestonelike monolayer, expressed Factor VIII related antigen, and incorporated acetylated-low density lipoprotein. The magnetic beads seemed not to modify the normal properties of the isolated endothelium, thus facilitating their use in experimental studies. This immunomagnetic separation technique may be applicable for purification of endothelial cells from a wide variety of tissue sources.
authors: Office of the Director, Division of Cancer Etiology, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 37, Room 2D-02, 20892, Bethesda, Maryland.
authors: D E
authors: DE
authors: Gomez
authors: Office of the Director, Division of Cancer Etiology, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 37, Room 2D-02, 20892, Bethesda, Maryland.
authors: J L
authors: JL
authors: Hartzler
authors: Office of the Director, Division of Cancer Etiology, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 37, Room 2D-02, 20892, Bethesda, Maryland.
authors: R H
authors: RH
authors: Corbitt
authors: Office of the Director, Division of Cancer Etiology, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 37, Room 2D-02, 20892, Bethesda, Maryland.
authors: A M
authors: AM
authors: Nason
authors: Office of the Director, Division of Cancer Etiology, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 37, Room 2D-02, 20892, Bethesda, Maryland.
authors: U P
authors: UP
authors: Thorgeirsson
keywords: culture; immunoisolation; monkey liver endothelium