title: A Xanthomonas oryzae pv. oryzae effector, XopR, associates with receptor-like cytoplasmic kinases and suppresses PAMP-triggered stomatal closure.
abstract: Receptor-like kinases (RLKs) play important roles in plant immunity signaling; thus, many are hijacked by pathogen effectors to promote successful pathogenesis. Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of rice leaf blight disease. The strain PXO99A has 18 non-TAL (transcription activation-like) effectors; however, their mechanisms of action and host target proteins remain largely unknown. Although the effector XopR from the Xoo strain MAFF311018 was shown to suppress PAMP-triggered immune responses in Arabidopsis, its target has not yet been identified. Here, we show that PXO99A XopR interacts with BIK1 at the plasma membrane. BIK1 is a receptor-like cytoplasmic kinase (RLCK) belonging to the RLK family of proteins and mediates PAMP-triggered stomatal immunity. In turn, BIK1 phosphorylates XopR. Furthermore, XopR suppresses PAMP-triggered stomatal closure in transgenic Arabidopsis expressing XopR. In addition, XopR is able to associate with RLCKs other than BIK1. These results suggest that XopR likely suppresses plant immunity by targeting BIK1 and other RLCKs.
authors: State Key Laboratory of Plant Genomics, Center for Agricultural Research Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei, 050021, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors: Shuangfeng
authors: S
authors: Wang
authors: State Key Laboratory of Plant Genomics, Center for Agricultural Research Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei, 050021, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors: Jianhang
authors: J
authors: Sun
authors: State Key Laboratory of Plant Genomics, Center for Agricultural Research Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei, 050021, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors: Fenggui
authors: F
authors: Fan
authors: State Key Laboratory of Plant Genomics, Center for Agricultural Research Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei, 050021, China.
authors: Zhaoyun
authors: Z
authors: Tan
authors: State Key Laboratory of Plant Genomics, Center for Agricultural Research Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei, 050021, China.
authors: Yanmin
authors: Y
authors: Zou
authors: State Key Laboratory of Plant Genomics, Center for Agricultural Research Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei, 050021, China. dplu@sjziam.ac.cn.
authors: Dongping
authors: D
authors: Lu
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D009154:Mutation; D012275:Oryza / Q000166:cytology / Q000382:microbiology; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000378:metabolism*; D010766:Phosphorylation; D054046:Plant Stomata / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D030821:Plants, Genetically Modified; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011523:Protoplasts / Q000166:cytology / Q000378:metabolism; D014974:Xanthomonas / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D000069452:Pathogen-Associated Molecular Pattern Molecules; D049452:Green Fluorescent Proteins; C509522:BIK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
keywords: BIK1; Xanthomonas; XopR; effector; immunity