title: Molecular characterization of a clinical Haemophilus parainfluenzae isolate with cefotaxime resistance and decreased susceptibility to fluoroquinolones.
abstract: We report an H. parainfluenzae clinical isolate resistant to cefotaxime and with decreased susceptibility to ciprofloxacin recovered from a patient with cystic fibrosis. The isolate had elevated MICs of ampicillin (256mg/L), amoxicillin-clavulanate (8mg/L), cefuroxime (8mg/L) and cefotaxime (4mg/L), and showed a β-lactamase-producing amoxicillin-clavulanic acid-resistant (BLPACR) phenotype. A blaTEM-1 plus five amino acid substitutions in the PBP3 were found: Ser385Thr, Val511Ala, Ile519Val, Asn526Lys and Asp551Leu. MIC of ciprofloxacin was 0.5mg/L, and substitutions in gyrA (Ser84Tyr) and parC (Ser84Phe) genes were detected.
authors: Servicio Antimicrobianos, National Reference Laboratory, Instituto Nacional de Enfermedades Infecciosas-ANLIS "Dr. Carlos G. Malbrán", Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors: Diego
authors: D
authors: Faccone
authors: Servicio Antimicrobianos, National Reference Laboratory, Instituto Nacional de Enfermedades Infecciosas-ANLIS "Dr. Carlos G. Malbrán", Argentina.
authors: Paula
authors: P
authors: Lopez-Ruitti
authors: Hospital General de Niños "Dr. Ricardo Gutierrez", Buenos Aires, Argentina.
authors: Miryam
authors: M
authors: Vazquez
authors: Servicio Antimicrobianos, National Reference Laboratory, Instituto Nacional de Enfermedades Infecciosas-ANLIS "Dr. Carlos G. Malbrán", Argentina.
authors: Leonor
authors: L
authors: Guerriero
authors: Servicio Antimicrobianos, National Reference Laboratory, Instituto Nacional de Enfermedades Infecciosas-ANLIS "Dr. Carlos G. Malbrán", Argentina.
authors: Celeste
authors: C
authors: Lucero
authors: Servicio Antimicrobianos, National Reference Laboratory, Instituto Nacional de Enfermedades Infecciosas-ANLIS "Dr. Carlos G. Malbrán", Argentina.
authors: Paula
authors: P
authors: Gagetti
authors: Servicio Antimicrobianos, National Reference Laboratory, Instituto Nacional de Enfermedades Infecciosas-ANLIS "Dr. Carlos G. Malbrán", Argentina.
authors: Paola
authors: P
authors: Ceriana
authors: Servicio Antimicrobianos, National Reference Laboratory, Instituto Nacional de Enfermedades Infecciosas-ANLIS "Dr. Carlos G. Malbrán", Argentina. Electronic address: acorso@anlis.gov.ar.
authors: Alejandra
authors: A
authors: Corso
mesh_terms: D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002439:Cefotaxime / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D024841:Fluoroquinolones / Q000494:pharmacology*; D005798:Genes, Bacterial; D006192:Haemophilus Infections / Q000382:microbiology*; D044135:Haemophilus parainfluenzae / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D002439:Cefotaxime
keywords: Cefotaxime; Ciprofloxacin; Haemophilus parainfluenzae; PBP3