title: [Not Available].
abstract: BACKGROUND
  Mutation analysis has identified a G-> A transition in the promoter region of TNF-alpha gene at position -308 (rs1800629).
  
  
  OBJECTIVE
  The aim of our study was to investigate the influence of polymorphism in -308 GA promoter variant of the TNF alpha gene on metabolic response and weight loss secondary to two hypocaloric diets.
  
  
  METHODS
  A sample of 283 obese subjects was enrolled in a consecutive prospective way. In the basal visit, patients were randomly allocated during 9 months to diet HP (high protein/low carbohydrate hypocaloric diet) and diet S (standard hypocaloric diet).
  
  
  RESULTS
  There were no significant differences between the positive effects on weight loss in either genotype group with both diets. With both diets and only in wild genotype (diet HP vs. diet S), total cholesterol (-9.1 ± 3.4 mg/dL vs. -6.9 ± 2.0 mg/dL; p > 0.05), LDL cholesterol (-9.0 ± 2.9 mg/dL vs. -6.5 ± 2.1 mg/dL; p > 0.05) and triglycerides (-23.1 ± 5.1 mg/dL vs. -12.3 ± 4.8 mg/dL; p < 0.05) decreased. The improvement in triglycerides was higher in subjects without A allele. With diet HP and only in wild genotype, insulin levels (-3.1 ± 1.8 UI/L; p < 0.05) and HOMA-R (-0.8 ± 0.1 units; p < 0.05) decreased.
  
  
  CONCLUSIONS
  Carriers of -308 GG promoter variant of TNF-alpha gene have a better metabolic response than -308 GA obese with a high protein hypocaloric diet.
authors: Institute of Endocrinology and Nutrition. Medicine School and Department of Endocrinology and Nutrition. Hospital Clínico Universitario de Valladolid. Universidad de Valladolid. dadluis@yahoo.es.
authors: Daniel A
authors: DA
authors: De Luis
authors: Rocío
authors: R
authors: Aller
authors: Olatz
authors: O
authors: Izaola
authors: Enrique
authors: E
authors: Romero
mesh_terms: D000328:Adult; D000886:Anthropometry; D050528:Diet, Carbohydrate-Restricted; D004038:Diet, Reducing; D004044:Dietary Proteins; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics; D011446:Prospective Studies; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D015431:Weight Loss / Q000235:genetics* / Q000502:physiology
chemical_list: D004044:Dietary Proteins; D014409:Tumor Necrosis Factor-alpha
keywords: G308 TNF-alpha polymorphism. Hypocaloric diet. Obesity.