title: Emerging roles of calcium-activated K channels and TRPV4 channels in lung oedema and pulmonary circulatory collapse.
abstract: It has been suggested that the transient receptor potential cation (TRP) channel subfamily V (vanilloid) type 4 (TRPV4) and intermediate conductance calcium-activated potassium (KCa3.1) channels contribute to endothelium-dependent vasodilation. Here, we summarize very recent evidence for a synergistic interplay of TRPV4 and KCa3.1 channels in lung disease. Among the endothelial Ca2+-permeable TRPs, TRPV4 is best characterized and produces arterial dilation by stimulating Ca2+-dependent nitric oxide synthesis and endothelium-dependent hyperpolarization. Besides these roles, some TRP channels control endothelial/epithelial barrier functions and vascular integrity, while KCa3.1 channels provide the driving force required for Cl-and water transport in some cells and most secretory epithelia. The three conditions, increased pulmonary venous pressure caused by left heart disease, high inflation pressure and chemically induced lung injury, may lead to activation of TRPV4 channels followed by Ca2+influx leading to activation of KCa3.1 channels in endothelial cells ultimately leading to acute lung injury. We find that a deficiency in KCa3.1 channels protects against TRPV4-induced pulmonary arterial relaxation, fluid extravasation, haemorrhage, pulmonary circulatory collapse and cardiac arrest in vivo. These data identify KCa3.1 channels as crucial molecular components in downstream TRPV4 signal transduction and as a potential target for the prevention of undesired fluid extravasation, vasodilatation and pulmonary circulatory collapse.
authors: Department of Biomedicine, Pulmonary and Cardiovascular Pharmacology, Aarhus University, Aarhus C, Denmark.
authors: U
authors: U
authors: Simonsen
authors: Department of Biomedicine, Pulmonary and Cardiovascular Pharmacology, Aarhus University, Aarhus C, Denmark.
authors: C
authors: C
authors: Wandall-Frostholm
authors: Translational Research Unit, University Hospital Miguel Servet and IACS/IIS, Aragonese Agency for Investigation and Development (ARAID), Zaragoza, Spain.
authors: A
authors: A
authors: Oliván-Viguera
authors: Translational Research Unit, University Hospital Miguel Servet and IACS/IIS, Aragonese Agency for Investigation and Development (ARAID), Zaragoza, Spain.
authors: R
authors: R
authors: Köhler
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000378:metabolism*; D024681:Potassium Channels, Calcium-Activated / Q000378:metabolism*; D011652:Pulmonary Circulation / Q000502:physiology*; D011654:Pulmonary Edema / Q000378:metabolism*; D050051:Transient Receptor Potential Channels / Q000378:metabolism*
chemical_list: D024681:Potassium Channels, Calcium-Activated; D050051:Transient Receptor Potential Channels; D002118:Calcium
keywords: KCa3.1; TRPV4; collapse; endothelium; lung oedema; pulmonary circulation