title: Association of the Single Nucleotide Polymorphisms inRUNX1,DYRK1A, andKCNJ15with Blood Related Traits in Pigs.
abstract: The aim of this study was to detect positional candidate genes located within the support interval (SI) regions based on the results of red blood cell, mean corpuscular volume (MCV), and mean corpuscular hemoglobin quantitative trait locus (QTL) inSus scrofachromosome 13, and to verify the correlation between specific single-nucleotide polymorphisms (SNPs) located in the exonic region of the positional candidate gene and the three genetic traits. The flanking markers of the three QTL SI regions areSW38andS0215. Within the QTL SI regions, 44 genes were located, and runt-related transcription factor 1, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A (DYRK1A), and potassium inwardly-rectifying channel, subfamily J, member 15KCNJ15-which are reported to be related to the hematological traits and clinical features of Down syndrome-were selected as positional candidate genes. The ten SNPs located in the exonic region of the three genes were detected by next generation sequencing. A total of 1,232 pigs of an F2resource population between Landrace and Korean native pigs were genotyped. To investigate the effects of the three genes on each genotype, a mixed-effect model which is the considering family structure model was used to evaluate the associations between the SNPs and three genetic traits in the F2intercross population. Among them, the MCV level was highly significant (nominal p = 9.8×10-9) in association with theDYRK1A-SNP1 (c.2989 G<A), and explained 4.9% of the phenotypic variance. However, since linkage disequilibrium is quite extensive in an F2intercross, our approach has limited power to distinguish one particular positional candidate gene from a QTL region.
authors: Korea Zoonosis Research Institute (KoZRI), Chonbuk National University, Iksan 54531, Korea.
authors: Jae-Bong
authors: JB
authors: Lee
authors: Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea.
authors: Chae-Kyoung
authors: CK
authors: Yoo
authors: National Institute of Animal Science, Rural Development Administration, Jeju 63242, Korea.
authors: Hee-Bok
authors: HB
authors: Park
authors: National Institute of Animal Science, Rural Development Administration, Jeju 63242, Korea.
authors: In-Cheol
authors: IC
authors: Cho
authors: Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea.|Division of Applied Life Science (BK21 plus), Graduate School of Gyeongsang National University, Jinju 52828, Korea.
authors: Hyun-Tae
authors: HT
authors: Lim
keywords: Association Test; Blood Related Traits; Korean Native Pigs; Landrace; Positional Candidate Gene