title: A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep.
abstract: Expression of Csf1r in adults is restricted to cells of the macrophage lineage. Transgenic reporters based upon the Csf1r locus require inclusion of the highly conserved Fms-intronic regulatory element for expression. We have created Csf1r-EGFP transgenic sheep via lentiviral transgenesis of a construct containing elements of the mouse Fms-intronic regulatory element and Csf1r promoter. Committed bone marrow macrophage precursors and blood monocytes express EGFP in these animals. Sheep monocytes were divided into three populations, similar to classical, intermediate, and nonclassical monocytes in humans, based upon CD14 and CD16 expression. All expressed EGFP, with increased levels in the nonclassical subset. Because Csf1r expression coincides with the earliest commitment to the macrophage lineage, Csf1r-EGFP bone marrow provides a tool for studying the earliest events in myelopoiesis using the sheep as a model.
authors: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom; and.
authors: Clare
authors: 0000-0001-9423-557X
authors: C
authors: Pridans
authors: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom; and.
authors: Gemma M
authors: 0000-0001-8323-5013
authors: GM
authors: Davis
authors: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom; and.
authors: Kristin A
authors: KA
authors: Sauter
authors: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom; and.
authors: Zofia M
authors: ZM
authors: Lisowski
authors: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom; and.
authors: Yolanda
authors: 0000-0002-1344-7405
authors: Y
authors: Corripio-Miyar
authors: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom; and.
authors: Anna
authors: A
authors: Raper
authors: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom; and.
authors: Lucas
authors: L
authors: Lefevre
authors: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom; and.
authors: Rachel
authors: R
authors: Young
authors: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom; and.
authors: Mary E
authors: ME
authors: McCulloch
authors: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom; and.
authors: Simon
authors: 0000-0002-5101-2277
authors: S
authors: Lillico
authors: Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian EH25 9RG, United Kingdom.
authors: Elspeth
authors: E
authors: Milne
authors: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom; and.
authors: Bruce
authors: 0000-0002-2918-1605
authors: B
authors: Whitelaw
authors: The Roslin Institute, University of Edinburgh, Midlothian EH25 9RG, United Kingdom; and David.Hume@roslin.ed.ac.uk.
authors: David A
authors: DA
authors: Hume
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000276:immunology*; D015415:Biomarkers / Q000097:blood*; D002454:Cell Differentiation; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D051379:Mice; D009000:Monocytes / Q000502:physiology*; D038042:Myelopoiesis; D011401:Promoter Regions, Genetic; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics*; D017452:Receptors, IgG / Q000235:genetics / Q000276:immunology; D012756:Sheep / Q000235:genetics; D019076:Transgenes
chemical_list: D015415:Biomarkers; C000604004:Csf1r protein, mouse; D018950:Lipopolysaccharide Receptors; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D017452:Receptors, IgG; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins