title: MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma.
abstract: Hepatic stellate cells (HSCs) induce immune privilege and promote hepatocellular carcinoma (HCC) by suppressing the immune system. On the other hand, galectin-1 and miRNA-22 (miR-22) are dysregulated in HCC and serve as prognostic indicators for patients. In this study, therefore, we measured galectin-1 and miR-22 expression in HSCs isolated from HCC tissues (Ca-HSCs), and in normal liver tissues (N-HSCs) as a control. We also investigated the apoptosis rate among T cells and the production of cytokines (IFN-γ and IL-10) in HSCs co-cultured with T cells. And we used immunohistochemical staining to tested for correlation between galectin-1 expression, CD3 expression and clinicopathological features in 162 HCC patients. Our results showed that galectin-1 expression was much higher in Ca-HSCs than in N-HSCs. Overexpression of galectin-1 promoted HSC-induced T cell apoptosis and cytokine production (IFN-γ and IL-10), while miR-22 expression inhibited it. Galectin-1 expression correlated negatively with miR-22 expression in HSCs. High galectin-1 and low CD3 expression levels were associated with poor prognosis in HCC patients. These results suggest that the immunosuppressive microenvironment promoted by HSC-derived galectin-1 in HCC can be inhibited by miR-22. Galectin-1 and miR-22 could potentially serve as prognostic markers and therapeutic targets in HCC.
authors: Department of Hepatobiliary Surgery Institute, South Western Hospital, Third Military Medical University, Chongqing 400038, China.
authors: Yu
authors: Y
authors: You
authors: Department of Hepatobiliary Surgery Institute, South Western Hospital, Third Military Medical University, Chongqing 400038, China.
authors: Jia-Xin
authors: JX
authors: Tan
authors: Department of Hepatobiliary Surgery Institute, South Western Hospital, Third Military Medical University, Chongqing 400038, China.
authors: Hai-Su
authors: HS
authors: Dai
authors: Department of Hepatobiliary Surgery Institute, South Western Hospital, Third Military Medical University, Chongqing 400038, China.
authors: Hao-Wei
authors: HW
authors: Chen
authors: Department of Hepatobiliary Surgery Institute, South Western Hospital, Third Military Medical University, Chongqing 400038, China.
authors: Xue-Jun
authors: XJ
authors: Xu
authors: Department of Hepatobiliary Surgery Institute, South Western Hospital, Third Military Medical University, Chongqing 400038, China.
authors: Ai-Gang
authors: AG
authors: Yang
authors: Department of Hepatobiliary Surgery Institute, South Western Hospital, Third Military Medical University, Chongqing 400038, China.
authors: Yu-Jun
authors: YJ
authors: Zhang
authors: Department of Hepatobiliary Surgery Institute, South Western Hospital, Third Military Medical University, Chongqing 400038, China.
authors: Lian-Hua
authors: LH
authors: Bai
authors: Department of Hepatobiliary Surgery Institute, South Western Hospital, Third Military Medical University, Chongqing 400038, China.
authors: Ping
authors: P
authors: Bie
mesh_terms: D000199:Actins / Q000378:metabolism; D000368:Aged; D017209:Apoptosis; D017252:CD3 Complex / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D005260:Female; D037483:Galectin 1 / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007372:Interferons / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D020014:K562 Cells; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D011379:Prognosis; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D000199:Actins; D017252:CD3 Complex; D016207:Cytokines; D037483:Galectin 1; C508609:IL10 protein, human; C445499:LGALS1 protein, human; C548375:MIRN22 microRNA, human; D035683:MicroRNAs; D016753:Interleukin-10; D007372:Interferons
keywords: galectin-1; hepatic stellate cells; hepatocellular carcinoma; miRNA-22