title: Re-evaluation of progenitor thresholds and expectations for haematopoietic recovery based on an analysis of 810 autologous transplants: Implications for quality assurance.
abstract: Haematological engraftment was assessed in 804 autologous transplants. Neutrophil recovery occurred in over 99% within 14 d but platelet recovery was delayed beyond this time in 14·8%. Time to recovery was dependent on the progenitor cell dose infused. The minimum CD34+cell threshold adopted in this study (2 × 106/kg) was safe although recovery was faster with a dose >5 × 106/kg. CD34+cell doses of between 1 and 2 × 106/kg were also acceptable if either the granulocyte-macrophage colony-forming cell dose exceeded 2 × 105/kg or this dose was due to splitting a higher yield harvest. Prompt neutrophil recovery affords important quality assurance for laboratory processing.
authors: Wolfson Cellular Therapy Unit, University College London Hospitals NHS Foundation Trust, London, UK.
authors: Michael J
authors: MJ
authors: Watts
authors: Wolfson Cellular Therapy Unit, University College London Hospitals NHS Foundation Trust, London, UK.
authors: Stuart J
authors: SJ
authors: Ings
authors: Wolfson Cellular Therapy Unit, University College London Hospitals NHS Foundation Trust, London, UK.
authors: Carmen
authors: C
authors: Balsa
authors: Wolfson Cellular Therapy Unit, University College London Hospitals NHS Foundation Trust, London, UK.
authors: Andrew
authors: A
authors: Antonio
authors: Wolfson Cellular Therapy Unit, University College London Hospitals NHS Foundation Trust, London, UK.
authors: Simon
authors: S
authors: Hack
authors: UCL, Cancer Institute, London, UK.
authors: David C
authors: DC
authors: Linch
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001772:Blood Cell Count; D005260:Female; D006085:Graft Survival; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods / Q000592:standards; D006412:Hematopoietic Stem Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D011785:Quality Assurance, Health Care; D013997:Time Factors; D019172:Transplantation Conditioning; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D055815:Young Adult
keywords: engraftment kinetics; progenitors; quality control; stem cell transplantation