title: Site-specific associations between miRNA expression and survival in colorectal cancer cases.
abstract: BACKGROUND
  MicroRNAs (miRNA) are small non-coding RNA involved in cellular processes, including cell proliferation and angiogenesis. Thus, miRNA expression may alter survival after diagnosis with colorectal cancer (CRC).
  
  
  RESULTS
  Individuals diagnosed with stage 1 or stage 2 rectal cancer had worse survival than colon cancer cases diagnosed at stage 1 or stage 2. After adjustment for multiple comparisons, no miRNAs were significantly associated with disease stage. Two miRNAs infrequently expressed in the population and not previously reported were associated with survival after diagnosis with colon cancer (miR-1 HR 2.17 95% CI 1.41, 3.36; and miR-101-3p HR 3.51 95% CI 1.72, 7.15). Among those diagnosed with rectal cancer, 201 miRNAs were associated with survival when the FDR q value was < 0.05. Assessment of 105 previously reported miRNAs associated with prognosis showed that four miRNAs influenced colon cancer survival and 17 influenced survival after a diagnosis with rectal cancer when raw p values were considered.
  
  
  METHODS
  This study includes data from population-based studies of CRC conducted in Utah and the Kaiser Permanente Medical Care Program. A total of 1893 carcinoma and normal paired colorectal mucosa tissue samples were run using the Agilent Human miRNA Microarray V19.0. We assessed miRNA differential expression between paired carcinoma and normal colonic mucosa tissue with CRC- specific survival evaluating stage and site-specific associations after adjusting for age, sex, microsatellite instability tumor status, and AJCC stage.
  
  
  CONCLUSIONS
  MiRNAs dysregulated for both colon and rectal cancer had a greater impact on survival after a diagnosis with rectal cancer.
authors: Department of Medicine, University of Utah, Salt Lake City, Utah 84108, USA.
authors: Martha L
authors: ML
authors: Slattery
authors: Department of Medicine, University of Utah, Salt Lake City, Utah 84108, USA.
authors: Jennifer S
authors: JS
authors: Herrick
authors: Department of Medicine, University of Utah, Salt Lake City, Utah 84108, USA.
authors: Daniel F
authors: DF
authors: Pellatt
authors: Department of Medicine, University of Utah, Salt Lake City, Utah 84108, USA.
authors: Lila E
authors: LE
authors: Mullany
authors: Department of Mathematics and Statistics, Utah State University, Logan, Utah 84322, USA.
authors: John R
authors: JR
authors: Stevens
authors: Department of Medicine, University of Utah, Salt Lake City, Utah 84108, USA.
authors: Erica
authors: E
authors: Wolff
authors: Department of Medicine, University of Utah, Salt Lake City, Utah 84108, USA.
authors: Michael D
authors: MD
authors: Hoffman
authors: Department of Medicine, University of Utah, Salt Lake City, Utah 84108, USA.
authors: Roger K
authors: RK
authors: Wolff
authors: Department of Pathology, University of Utah School, Salt Lake City, Utah 84108, USA.
authors: Wade
authors: W
authors: Samowitz
mesh_terms: D000368:Aged; D003106:Colon / Q000378:metabolism / Q000473:pathology; D003110:Colonic Neoplasms / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D012004:Rectal Neoplasms / Q000235:genetics*; D012007:Rectum / Q000378:metabolism / Q000473:pathology
chemical_list: D035683:MicroRNAs
keywords: colon cancer; disease stage; miRNA; rectal cancer