title: Molecular cloning of glucose transporter 1 in grouper Epinephelus coioides and effects of an acute hyperglycemia stress on its expression and glucose tolerance.
abstract: The glucose transporter family proteins play pivotal roles in glucose metabolism. In this study, we successfully cloned the orange spotted grouper (Epinephelus coioides) glucose transporter 1 (EcGlut1) gene (GenBank accession: JQ623903). The full-length EcGlut1 cDNA was 2126 bp with a 1476 bp ORF, a 437bp5'-UTR and 223bp3'-UTR. EcGlut1 is predicted to encode a 491 amino acid protein with a MW of 53.9 kDa, a pI of 8.66 and a Pfam domain. Bioinformatics analysis revealed that EcGlut1 was evolutionally conserved between fishes with 80-89 % amino acid identities. EcGlut1 was expressed predominantly in heart and liver and at lower levels in muscle, intestine, stomach and brain. We also investigated the effect of acute hyperglycemia stress on EcGlut1 expression. In glucose tolerance test, changes in EcGlut1 mRNA expression in response to glucose injection and glucose metabolism-related indictors were assessed at the same time. Glucose injection significantly suppressed EcGlut1 mRNA expression in liver at 12 h and in brain at 24 h postinjection (P < 0.05). EcGlut1 mRNA levels in heart were increased at 6 h (P < 0.05). Plasma glucose level increased significantly and reached its maximum at 3 h postinjection (P < 0.05). The spatiotemporal expression of EcGlut1 and glucose metabolism suggested that orange spotted grouper might rely on fat anabolism to reduce acute hyperglycemia stress and the delayed transcription of EcGlut1 gene might be one reason for glucose intolerance in E. coioides.
authors: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, People's Republic of China.|Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, People's Republic of China.
authors: Hongyu
authors: H
authors: Liu
authors: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, People's Republic of China.
authors: Xiaohui
authors: X
authors: Dong
authors: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, People's Republic of China.
authors: Shuyan
authors: S
authors: Chi
authors: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, People's Republic of China.
authors: Qihui
authors: Q
authors: Yang
authors: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, People's Republic of China.
authors: Shuang
authors: S
authors: Zhang
authors: College of Life Sciences, East China Normal University, Shanghai, 200062, People's Republic of China.
authors: Liqiao
authors: L
authors: Chen
authors: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, People's Republic of China. bptan@126.com.
authors: Beiping
authors: B
authors: Tan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001786:Blood Glucose / Q000032:analysis; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005393:Fish Diseases / Q000097:blood / Q000235:genetics*; D005947:Glucose / Q000493:pharmacokinetics / Q000494:pharmacology; D051272:Glucose Transporter Type 1 / Q000235:genetics*; D006943:Hyperglycemia / Q000097:blood / Q000235:genetics* / Q000662:veterinary; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D018076:DNA, Complementary; D051272:Glucose Transporter Type 1; D012333:RNA, Messenger; D014280:Triglycerides; D005947:Glucose
keywords: Epinephelus coioides; Glucose metabolism; Glucose transporter 1; Hyperglycemia; Molecular cloning