title: Vaccine potential of recombinant cathepsinL1G against Fasciola gigantica in mice.
abstract: In this study, we characterized and investigated the vaccine potential of FgCatL1G against Fasciola gigantica infection in mice. Recombinant mature FgCatL1G (rmFgCatL1G) was expressed in Escherichia coli BL21. The vaccination was performed in Imprinting Control Region (ICR) mice (n=10) by subcutaneous injection with 50μg of rmFgCatL1G combined with Freund's adjuvant. Two weeks after the second boost, mice were infected with 15 metacercariae by the oral route. The percents of protection of rmFgCatL1G vaccine were estimated to be 56.5% and 58.3% when compared with non vaccinated-infected and adjuvant-infected controls, respectively. Antibodies in the immune sera of vaccinated mice were shown by immunoblot to react with the native FgCatL1s in the extract of all stages of parasites and rmFgCatL1H, recombinant pro - FgCatL1 (rpFgCatL1). By immunohistochemistry, the immune sera also reacted with FgCatL1s in the caecal epithelial cells of the parasites. The levels of IgG1 and IgG2a in the immune sera, which are indicative of Th2 and Th1 immune responses, were also increased with IgG1 predominating. The levels of serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) in rmFgCatL1G-immunized group showed no significant difference from the control groups, but pathological lesions of livers in rmFgCatL1G-immunized group showed significant decrease when compared to the control groups. This study indicates that rmFgCatL1G has a vaccine potential against F. gigantica in mice, and this potential will be tested in larger livestock animals.
authors: Faculty of Allied Health Sciences, Burapha University, Long-Hard Bangsaen Road, Mueang District, Chonburi 20131, Thailand.
authors: Narin
authors: N
authors: Changklungmoa
authors: Faculty of Allied Health Sciences, Burapha University, Long-Hard Bangsaen Road, Mueang District, Chonburi 20131, Thailand.
authors: Natthacha
authors: N
authors: Phoinok
authors: Faculty of Allied Health Sciences, Burapha University, Long-Hard Bangsaen Road, Mueang District, Chonburi 20131, Thailand.
authors: Chonthicha
authors: C
authors: Yencham
authors: Faculty of Allied Health Sciences, Burapha University, Long-Hard Bangsaen Road, Mueang District, Chonburi 20131, Thailand; Department of Anatomy, Faculty of Science, Mahidol University, Rama 6 Road, Ratchathewi, Bangkok 10400, Thailand.
authors: Prasert
authors: P
authors: Sobhon
authors: Faculty of Allied Health Sciences, Burapha University, Long-Hard Bangsaen Road, Mueang District, Chonburi 20131, Thailand. Electronic address: earn_patho@hotmail.com.
authors: Pornanan
authors: P
authors: Kueakhai
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D002403:Cathepsins / Q000276:immunology*; D002417:Cattle; D006224:Cricetinae; D003546:Cysteine Endopeptidases / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005209:Fasciola / Q000276:immunology*; D005211:Fascioliasis / Q000473:pathology / Q000517:prevention & control*; D015801:Helminth Proteins / Q000276:immunology*; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood; D008099:Liver / Q000473:pathology; D008195:Lymnaea / Q000469:parasitology; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008813:Mice, Inbred ICR; D014612:Vaccines; D014614:Vaccines, Synthetic
chemical_list: D000909:Antibodies, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G; D014612:Vaccines; D014614:Vaccines, Synthetic; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D002403:Cathepsins; C086102:cat-L1B protein, Fasciola gigantica; D003546:Cysteine Endopeptidases
keywords: Cathepsin L1G; Characterization; Fasciola gigantica; Mice; Protection; Vaccine