title: Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models.
abstract: Although bioluminescence imaging (BLI) shows promise for monitoring tumor burden in animal models of cancer, these analyses remain mostly qualitative. Here we describe a method for bioluminescence imaging to obtain a semi-quantitative analysis of tumor burden and treatment response. This method is based on the calculation of a luminoscore, a value that allows comparisons of two animals from the same or different experiments. Current BLI instruments enable the calculation of this luminoscore, which relies mainly on the acquisition conditions (back and front acquisitions) and the drawing of the region of interest (manual markup around the mouse). Using two previously described mouse lymphoma models based on cell engraftment, we show that the luminoscore method can serve as a noninvasive way to verify successful tumor cell inoculation, monitor tumor burden, and evaluate the effects of in situ cancer treatment (CpG-DNA). Finally, we show that this method suits different experimental designs. We suggest that this method be used for early estimates of treatment response in preclinical small-animal studies.
authors: Genethon; Cordeliers Research Center, INSERM U872; Matter and Complex Systems Laboratory, Université Paris Diderot; jcosette@genethon.fr.
authors: Jeremie
authors: J
authors: Cosette
authors: Cordeliers Research Center, INSERM U872.
authors: Rym
authors: R
authors: Ben Abdelwahed
authors: Genethon; INSERM U951; UMR_S951, Université d'Evry Val d'Essonne.
authors: Sabrina
authors: S
authors: Donnou-Triffault
authors: Cordeliers Research Center, INSERM U872.
authors: Catherine
authors: C
authors: Sautès-Fridman
authors: Matter and Complex Systems Laboratory, Université Paris Diderot.
authors: Patrice
authors: P
authors: Flaud
authors: Genethon; INSERM U951; UMR_S951, Université d'Evry Val d'Essonne.
authors: Sylvain
authors: S
authors: Fisson
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D008163:Luminescent Measurements; D008223:Lymphoma; D051379:Mice