title: Evidence of protein-free homology recognition in magnetic bead force-extension experiments.
abstract: Earlier theoretical studies have proposed that the homology-dependent pairing of large tracts of dsDNA may be due to physical interactions between homologous regions. Such interactions could contribute to the sequence-dependent pairing of chromosome regions that may occur in the presence or the absence of double-strand breaks. Several experiments have indicated the recognition of homologous sequences in pure electrolytic solutions without proteins. Here, we report single-molecule force experiments with a designed 60 kb long dsDNA construct; one end attached to a solid surface and the other end to a magnetic bead. The 60 kb constructs contain two 10 kb long homologous tracts oriented head to head, so that their sequences match if the two tracts fold on each other. The distance between the bead and the surface is measured as a function of the force applied to the bead. At low forces, the construct molecules extend substantially less than normal, control dsDNA, indicating the existence of preferential interaction between the homologous regions. The force increase causes no abrupt but continuous unfolding of the paired homologous regions. Simple semi-phenomenological models of the unfolding mechanics are proposed, and their predictions are compared with the data.
authors: Department of Chemistry , Imperial College London , London SW7 2AZ, UK.
authors: D J
authors: DJ
authors: O' Lee
authors: Department of Physics , Harvard University, Cambridge , MA 02138, USA.
authors: C
authors: C
authors: Danilowicz
authors: Department of Chemistry , Imperial College London , London SW7 2AZ, UK.
authors: C
authors: C
authors: Rochester
authors: Department of Chemistry , Imperial College London , London SW7 2AZ, UK.
authors: A A
authors: 0000-0002-3157-8791
authors: AA
authors: Kornyshev
authors: Department of Physics , Harvard University, Cambridge , MA 02138, USA.
authors: M
authors: M
authors: Prentiss
keywords: DNA; homology recognition; macromolecular mechanics; molecular interactions; single-molecule force experiments