title: Modulating luminescence of Tb(3+) with biomolecules for sensing heparin and its contaminant OSCS.
abstract: The detection of heparin (Hep) and its contaminant oversulfated chondroitin sulfate (OSCS) is of great importance in clinics but remains challenging. Here, we report a sensitive and selective time-resolved luminescence (TRL) biosensing system for Hep by modulating the photoluminescence of Tb(3+) with guanine-rich ssDNA and Hep-specific AG73 peptide (RKRLQVQLSIRT). With the developed system, Hep including both unfractionated Hep (UFH) and the low molecular weight Hep (LMWH) has been successfully detected with a satisfactory detection limit. Owing to the highly specific interaction between Hep and AG73 peptide, major interfering substances in Hep detection, such as Hep analogs of chondrotin sulfate (Chs) and hyaluronic acid (HA), did not interfere with Hep detection. The established TRL sensing system was then successfully used for monitoring Hep metabolism in living rats by microdialysis. Moreover, the proposed TRL sensing system was further applied to analyze OSCS contaminant in Hep with heparinases treatment by exploring the inhibition effects of OSCS on the activity of heparinases. As low as 0.002% of OSCS in Hep was identified.
authors: Department of Biomedical Engineering, College of Engineering and Applied Sciences, Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, 210093 China.
authors: Yihui
authors: Y
authors: Hu
authors: Department of Biomedical Engineering, College of Engineering and Applied Sciences, Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, 210093 China.
authors: Wenjing
authors: W
authors: Guo
authors: Department of Biomedical Engineering, College of Engineering and Applied Sciences, Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, 210093 China.
authors: Yubin
authors: Y
authors: Ding
authors: Department of Biomedical Engineering, College of Engineering and Applied Sciences, Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, 210093 China.
authors: Hanjun
authors: H
authors: Cheng
authors: Department of Biomedical Engineering, College of Engineering and Applied Sciences, Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, 210093 China. Electronic address: weihui@nju.edu.cn.
authors: Hui
authors: H
authors: Wei
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry; D002809:Chondroitin Sulfates / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D004340:Drug Contamination; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006493:Heparin / Q000032:analysis* / Q000097:blood* / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013725:Terbium / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D013725:Terbium; D006493:Heparin; D002809:Chondroitin Sulfates
keywords: Heparin; Luminescence probes; Oversulfated chondroitin sulfate; Tb(3+); Time-resolved luminescence