title: Interfibrillar packing of bovine cornea by table-top and synchrotron scanning SAXS microscopy.
abstract: Bovine cornea was studied with scanning small-angle X-ray scattering (SAXS) microscopy, by using both synchrotron radiation and a microfocus laboratory source. A combination of statistical (adaptive binning and canonical correlation analysis) and crystallographic (pair distribution function analysis) approaches allowed inspection of the collagen lateral packing of the supramolecular structure. Results reveal (i) a decrease of the interfibrillar distance and of the shell thickness around the fibrils from the periphery to the center of the cornea, (ii) a uniform fibril diameter across the explored area, and (iii) a distorted quasi-hexagonal arrangement of the collagen fibrils. The results are in agreement with existing literature. The overlap between laboratory and synchrotron-radiation data opens new perspectives for further studies on collagen-based/engineered tissues by the SAXS microscopy technique at laboratory-scale facilities.
authors: Istituto di Cristallografia (IC-CNR), via Amendola 122/O, Bari, I-70126, Italy.
authors: T
authors: T
authors: Sibillano
authors: Istituto di Cristallografia (IC-CNR), via Amendola 122/O, Bari, I-70126, Italy.
authors: L
authors: L
authors: De Caro
authors: IOM CNR Laboratorio TASC , Area Science Park - Basovizza, Bld MM SS 14, Trieste, 34149, Italy.
authors: F
authors: F
authors: Scattarella
authors: Fischell Department of Bioengineering, University of Maryland , College Park, MD 20742, USA.
authors: G
authors: G
authors: Scarcelli
authors: Istituto di Cristallografia (IC-CNR), via Amendola 122/O, Bari, I-70126, Italy.
authors: D
authors: D
authors: Siliqi
authors: Istituto di Cristallografia (IC-CNR), via Amendola 122/O, Bari, I-70126, Italy.
authors: D
authors: D
authors: Altamura
authors: Paul Scherrer Institut, Swiss Light Source , 5232, Villigen PSI, Switzerland.
authors: M
authors: M
authors: Liebi
authors: Istituto di Cristallografia (IC-CNR), via Amendola 122/O, Bari, I-70126, Italy.
authors: M
authors: M
authors: Ladisa
authors: Fischell Department of Bioengineering, University of Maryland , College Park, MD 20742, USA.
authors: O
authors: O
authors: Bunk
authors: Istituto di Cristallografia (IC-CNR), via Amendola 122/O, Bari, I-70126, Italy.
authors: C
authors: C
authors: Giannini
keywords: bovine cornea; scanning SAXS microscopy; small-angle X-ray scattering