title: Allergen-specific immune response suppresses interleukin 10 expression in B cells via increasing micro-RNA-17-92 cluster.
abstract: Interleukin (IL)-10-expressing B cells play a critical role in the immune homeostasis in the body; its regulation has not been fully understood. Micro-RNA (miR)-17-92 cluster has strong regulation in the immunity. This study tests a hypothesis that miR-17-92 cluster suppresses IL-10 expression in B cells. In this study, peripheral B cells were collected from patients with allergic rhinitis (AR). The B cells were treated with specific allergens, dust mite extracts, in the culture. The expressions of miR-17-92 cluster and IL-10 in the culture were assessed by real-time quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. The results showed that the levels of miR-19a, but not the rest of the 5 members (miR-17, miR-18a, miR-19b, miR-20a, and miR-92a), were significantly higher in peripheral B cells from AR patients as than in B cells from healthy participants. Exposure of B cells from AR patients to specific allergen, dust mite extracts, significantly increased the levels if miR-19a and suppressed the expression of IL-10 in B cells. The levels of histone deacetylase 11 and acetylated H3K9 were higher, and the RNA polymerase II and c-Maf (the IL-10 transcription factor) were lower, at the IL-10 promoter locus. In conclusion, miR-19a mediates the allergen-specific immune response-decreased IL-10 expression in B cells.
authors: ENT Institute of the Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Longgang ENT Hospital, Shenzhen, China.
authors: Xiao-Rui
authors: XR
authors: Geng
authors: ENT Institute of the Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Longgang ENT Hospital, Shenzhen, China.
authors: Shu-Qi
authors: SQ
authors: Qiu
authors: ENT Institute of the Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Longgang ENT Hospital, Shenzhen, China.
authors: Li-Tao
authors: LT
authors: Yang
authors: ENT Institute of the Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Longgang ENT Hospital, Shenzhen, China.
authors: Zhi-Qiang
authors: ZQ
authors: Liu
authors: ENT Institute of the Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Longgang ENT Hospital, Shenzhen, China.
authors: Gui
authors: G
authors: Yang
authors: ENT Institute of the Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Longgang ENT Hospital, Shenzhen, China.
authors: Jiang-Qi
authors: JQ
authors: Liu
authors: ENT Institute of the Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors: Lu
authors: L
authors: Zeng
authors: ENT Institute of the Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors: Xiao-Xi
authors: XX
authors: Li
authors: ENT Institute of the Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Longgang ENT Hospital, Shenzhen, China.
authors: Li-Hua
authors: LH
authors: Mo
authors: ENT Institute of the Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors: Zhi-Gang
authors: ZG
authors: Liu
authors: ENT Institute of the Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors: Ping-Chang
authors: PC
authors: Yang
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D004247:DNA / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D056426:Genetic Loci; D006801:Humans; D007109:Immunity; D016753:Interleukin-10 / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D062085:RNA, Long Noncoding; D065631:Rhinitis, Allergic / Q000235:genetics / Q000276:immunology
chemical_list: D000485:Allergens; C568997:MIR17HG, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D016753:Interleukin-10; D004247:DNA
keywords: B cell; allergy; interleukin-10; micro-RNA; mite allergen