title: Silicimonas algicola gen. nov., sp. nov., a member of the Roseobacter clade isolated from the cell surface of the marine diatom Thalassiosira delicatula.
abstract: A Gram-negative, aerobic, non-motile bacterium, designated strain KC90BT, was isolated from the surface of a cell of the marine diatom Thalassiosira delicatula. The bacterial cells were pleomorphic and formed very small, beige colonies on marine agar. Optimal growth was obtained at 25 °C, at pH 6.5-7.5 and in the presence of 1.5-2.0 % (w/v) NaCl. Phylogenetic analyses based on its 16S rRNA gene sequence revealed that strain KC90BT belonged to the Roseobacter clade and formed a monophyletic cluster with the sequences of Boseongicola aestuarii, Profundibacterium mesophilum, Hwanghaeicola aestuarii, Maribius pelagius and M. salinus, showing 91.4-95.7 % sequence similarities. Ubiquinone Q-10 was the predominant lipoquinone but a significant amount of ubiquinone Q-9 was also detected. The major cellular fatty acids were C18 : 1ω7c, 11-methyl C18 : 1ω7c and C18 : 0. Strain KC90BT also contained specific fatty acids (C17 : 0, anteiso-C15 : 0 and anteiso-C17 : 0) that were not detected in its closest described relatives. The major polar lipids of strain KC90BT comprised phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol and an unidentified aminolipid. The DNA G+C content of strain KC90BT was 65.2 mol%. The phylogenetic analysis of strain KC90BT, together with the differential phenotypic and chemotaxonomic properties demonstrate that strain KC90BT is distinct from type strains of B. aestuarii, P. mesophilum, H. aestuarii, M. pelagius and M. salinus. Based on the data presented in this study, strain KC90BT represents a novel genus and species within the family Rhodobacteraceae, for which the name Silicimonas algicola gen. nov., sp. nov. is proposed. The type strain is KC90BT (=DSM 103371T=RCC 4681T).
authors: CNRS, Station Biologique de Roscoff, Adaptation and Diversité en Milieu Marin, Marine Phototrophic Prokaryotes Team, Roscoff, France.|Sorbonne Universités, UPMC Univ Paris 06, Station Biologique de Roscoff, Adaptation et Diversité en Milieu Marin, Oceanic Plankton Group, Roscoff, France.
authors: Klervi
authors: K
authors: Crenn
authors: CNRS, Station Biologique de Roscoff, Adaptation and Diversité en Milieu Marin, Marine Phototrophic Prokaryotes Team, Roscoff, France.|Sorbonne Universités, UPMC Univ Paris 06, Station Biologique de Roscoff, Adaptation et Diversité en Milieu Marin, Oceanic Plankton Group, Roscoff, France.
authors: Delphine
authors: D
authors: Serpin
authors: Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
authors: Cendrella
authors: C
authors: Lepleux
authors: Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
authors: Jörg
authors: J
authors: Overmann
authors: CNRS, Station Biologique de Roscoff, Adaptation and Diversité en Milieu Marin, Marine Phototrophic Prokaryotes Team, Roscoff, France.|Sorbonne Universités, UPMC Univ Paris 06, Station Biologique de Roscoff, Adaptation et Diversité en Milieu Marin, Oceanic Plankton Group, Roscoff, France.
authors: Christian
authors: C
authors: Jeanthon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D017377:Diatoms / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2; C030571:ubiquinone 9