title: Isolation and characterization of bacteria from soil contaminated with diesel oil and the possible use of these in autochthonous bioaugmentation.
abstract: Two bacterial species (isolates N and O) were isolated from a paddy soil microcosm that had been artificially contaminated with diesel oil to which extrinsic Pseudomonas aeruginosa strain WatG, had been added exogenously. One bacterial species (isolate J) was isolated from a similar soil microcosm that had been biostimulated with Luria-Bertani (LB) medium. Isolates N and O, which were tentatively identified as Stenotrophomonas sp. and Ochromonas sp., respectively, by sequencing of their 16 S rRNA genes had no ability to degrade diesel oil on their own in any liquid medium. When each strain was cocultivated with P. aeruginosa strain WatG in liquid mineral salts medium (MSM) containing 1% diesel oil, isolate N enhanced the degradation of diesel oil by P. aeruginosa strain WatG, but isolate O inhibited it. In contrast, isolate J, which was tentatively identified as a Rhodococcus sp., degraded diesel oil contained not only in liquid LB and MSM, but also in paddy soil microcosms supplemented with LB medium. The bioaugmentation capacity of isolate J in soil microcosms contaminated with diesel oil was much higher than that of P. aeruginosa strain WatG. The possibility of using isolate J for autochthonous bioaugmentation is discussed.
authors: Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo, 060-0810, Japan.|ROM Co. Ltd., Chuo-ku, Sapporo, 064-0804, Japan.
authors: Akio
authors: A
authors: Ueno
authors: ROM Co. Ltd., Chuo-ku, Sapporo, 064-0804, Japan.
authors: Yukiya
authors: Y
authors: Ito
authors: National Institute of Advanced Industrial Science and Technology (AIST), Toyohira-ku, Sapporo, 062-8517, Japan.
authors: Isao
authors: I
authors: Yumoto
authors: Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo, 060-0810, Japan. hoku@ees.hokudai.ac.jp.
authors: Hidetoshi
authors: H
authors: Okuyama
keywords: Autochthonous bioaugmentation; Biostimulation; Diesel oil degradation; Soil intrinsic bacteria; Soil microcosms