title: The Effect of Protandim® Supplementation on Athletic Performance and Oxidative Blood Markers in Runners.
abstract: The purpose of this study determined if oral supplementation of Protandim® (a nutraceutical) for 90 days improved 5-km running performance and reduced serum thiobarbituric acid-reacting substances (TBARS) at rest, an indicator of oxidative stress. Secondary objectives were to measure whole blood superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GPX), at rest and 10 minutes after completion of the race before and after supplementation as well as quality of life. In a double-blind, randomized, placebo controlled trial, 38 runners [mean (SD) = 34 (7) yrs; BMI = 22 (2) kg/m2] received either 90 days of Protandim® [1 pill a day, n = 19)] or placebo (n = 19). Randomization was done in blocks of two controlling for sex and 5-km baseline performance. A 5-km race was performed at baseline and after 90 days of supplementation, with blood samples taken before and 10-min after each race. Fasting blood samples were acquired at baseline, after 30, 60, and 90 days of supplementation. TBARS, SOD, GPX, and GSH were assayed in an out-of-state accredited lab. Running performance was not altered by Protandim® or placebo [20.3 (2.1) minutes, with an -8 (33) seconds change in 5-km time regardless of group]. There was no change in TBARS, SOD, or GPX (at rest) after three months of Protandim® supplementation compared to placebo. However, in a subgroup ≥ 35 years of age, there was a 2-fold higher increase in SOD in those taking Protandim® for three months compared to those on placebo (p = 0.038). The mean post-race change in TBARS (compared to pre-race) increased by about 20% in half of the subjects, but was not altered between groups, even after three months of supplementation. Quality of life was also not different between the two conditions. In conclusion, Protandim® did not (1) alter 5-km running time, (2) lower TBARS at rest (3) raise antioxidant enzyme concentrations compared to placebo (with exception of SOD in those ≥ 35 years old) or, (4) affect quality of life compared to placebo.
  
  
  BACKGROUND
  ClinicalTrials.gov NCT02172625.
authors: Department of Health and Sport Sciences, University of Louisville, Louisville, KY, United States of America.
authors: Seteena L
authors: SL
authors: Ueberschlag
authors: Department of Health and Sport Sciences, University of Louisville, Louisville, KY, United States of America.
authors: James R
authors: JR
authors: Seay
authors: Department of Health and Sport Sciences, University of Louisville, Louisville, KY, United States of America.
authors: Alexandra H
authors: AH
authors: Roberts
authors: Department of Medicine, University of Louisville, Louisville, KY, United States of America.
authors: Pamela C
authors: PC
authors: DeSpirito
authors: Department of Clinical Sciences, University of Kentucky, Lexington, KY, United States of America.
authors: Jeremy M
authors: JM
authors: Stith
authors: Department of Medicine, Case Western Reserve University, Cleveland, OH, United States of America.
authors: Rodney J
authors: RJ
authors: Folz
authors: Department of Health and Sport Sciences, University of Louisville, Louisville, KY, United States of America.
authors: Kathleen A
authors: KA
authors: Carter
authors: Department of Nutrition and Dietetics, Saint Louis University, Saint Louis, MO, United States of America.
authors: Edward P
authors: EP
authors: Weiss
authors: Department of Respiratory Therapy, Georgia State University, Atlanta, GA, United States of America.
authors: Gerald S
authors: http://orcid.org/0000-0002-4473-1601
authors: GS
authors: Zavorsky
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D054874:Athletic Performance; D019587:Dietary Supplements; D004311:Double-Blind Method; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D011788:Quality of Life; D012420:Running; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; C509374:Protandim; D017392:Thiobarbituric Acid Reactive Substances; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione