title: Nanomechanical sandwich assay for multiple cancer biomarkers in breast cancer cell-derived exosomes.
abstract: The use of exosomes as cancer diagnostic biomarkers is technically limited by their size, heterogeneity and the need for extensive purification and labelling. We report the use of cantilever arrays for simultaneous detection of multiple exosomal surface-antigens with high sensitivity and selectivity. Exosomes from breast cancer were selectively identified by detecting over-expressed membrane-proteins CD24, CD63, and EGFR. Excellent selectivity however, was achieved when targeting the cell-surface proteoglycan, Glypican-1 at extraordinary limits (∼200 exosomes per mL, ∼0.1 pg mL(-1)).
authors: Faculty of Pharmacy and Pharmaceutical Sciences University of Alberta, Edmonton, Alberta T6G 2E1, Canada and Department of Chemical and Materials Engineering University of Alberta, Edmonton, T6G 2V4, Alberta, Canada. Thundat@ualberta.ca.
authors: H
authors: H
authors: Etayash
authors: Department of Chemical and Materials Engineering University of Alberta, Edmonton, T6G 2V4, Alberta, Canada. Thundat@ualberta.ca.
authors: A R
authors: AR
authors: McGee
authors: Faculty of Pharmacy and Pharmaceutical Sciences University of Alberta, Edmonton, Alberta T6G 2E1, Canada and Chapman University School of Pharmacy (CUSP), Harry and Diane Rinker Health Science Campus, Chapman University, Irvine, California 92618-1908, USA.
authors: K
authors: K
authors: Kaur
authors: Department of Chemical and Materials Engineering University of Alberta, Edmonton, T6G 2V4, Alberta, Canada. Thundat@ualberta.ca.
authors: T
authors: T
authors: Thundat
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000378:metabolism*; D051927:CD24 Antigen / Q000378:metabolism*; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000378:metabolism*; D055354:Exosomes / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles; D060149:Tetraspanin 30 / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D051927:CD24 Antigen; C554586:CD63 protein, human; D060149:Tetraspanin 30; C512478:EGFR protein, human; D066246:ErbB Receptors