title: Isolation, structural analysis, and expression characteristics of the maize nuclear factor Y gene families.
abstract: NUCLEAR FACTOR-Y (NF-Y) has been shown to play an important role in growth, development, and response to environmental stress. A NF-Y complex, which consists of three subunits, NF-YA, NF-YB, and, NF-YC, binds to CCAAT sequences in a promoter to control the expression of target genes. Although NF-Y proteins have been reported in Arabidopsis and rice, a comprehensive and systematic analysis of ZmNF-Y genes has not yet been performed. To examine the functions of ZmNF-Y genes in this family, we isolated and characterized 50 ZmNF-Y (14 ZmNF-YA, 18 ZmNF-YB, and 18 ZmNF-YC) genes in an analysis of the maize genome. The 50 ZmNF-Y genes were distributed on all 10 maize chromosomes, and 12 paralogs were identified. Multiple alignments showed that maize ZmNF-Y family proteins had conserved regions and relatively variable N-terminal or C-terminal domains. The comparative syntenic map illustrated 40 paralogous NF-Y gene pairs among the 10 maize chromosomes. Microarray data showed that the ZmNF-Y genes had tissue-specific expression patterns in various maize developmental stages and in response to biotic and abiotic stresses. The results suggested that ZmNF-YB2, 4, 8, 10, 13, and 16 and ZmNF-YC6, 8, and 15 were induced, while ZmNF-YA1, 3, 4, 6, 7, 10, 12, and 13, ZmNF-YB15, and ZmNF-YC3 and 9 were suppressed by drought stress. ZmNF-YA3, ZmNF-YA8 and ZmNF-YA12 were upregulated after infection by the three pathogens, while ZmNF-YA1 and ZmNF-YB2 were suppressed. These results indicate that the ZmNF-Ys may have significant roles in the response to abiotic and biotic stresses.
authors: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, China.
authors: Zhongbao
authors: Z
authors: Zhang
authors: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, China.
authors: Xianglong
authors: X
authors: Li
authors: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, China.
authors: Chun
authors: C
authors: Zhang
authors: College of Agriculture, Yangtze University, Hubei Collaborative Innovation Center for Grain Industry, Jingzhou 434023, Hubei, China.
authors: Huawen
authors: H
authors: Zou
authors: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, China; College of Agriculture, Yangtze University, Hubei Collaborative Innovation Center for Grain Industry, Jingzhou 434023, Hubei, China. Electronic address: zwu22@126.com.
authors: Zhongyi
authors: Z
authors: Wu
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D001487:Basidiomycota / Q000472:pathogenicity / Q000502:physiology; D023081:CCAAT-Binding Factor / Q000145:classification / Q000235:genetics*; D032461:Chromosomes, Plant / Q000737:chemistry*; D017124:Conserved Sequence; D055864:Droughts; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D046228:Microarray Analysis; D005810:Multigene Family; D012275:Oryza / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D016415:Sequence Alignment; D013312:Stress, Physiological; D026801:Synteny; D003313:Zea mays / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology
chemical_list: D023081:CCAAT-Binding Factor; D010940:Plant Proteins
keywords: Expression profiling; Gene duplication; Maize; NF-Y transcription factor; Stress