title: Concentration of Tumor Necrosis Factor-α and Interleukin-1β in Isolated Porcine Liver Depending on Type of Transgenesis.
abstract: BACKGROUND
  Transgenic animals may serve as organ donors in human organ transplantation. However, the number of the studies addressing all doubts related to this issue is currently insufficient for the clinical application of this approach. The aim of this study was to analyze the hepatic tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) synthesis during a 24-hour cold preservation of the transgenic pig liver, depending on the type of transgenesis.
  
  
  METHODS
  The study was carried out on wild-type and transgenic pig livers with transferred human α1,2-fucosyltransferase (FUT) and/or α-galactosidase (GAL) gene (four groups; n = 6). Harvested livers were perfused for 30 minutes and stored for 24 hours in Biolasol (Biochefa) solution at 4°C with a subsequent 30-minute reperfusion (reflush). TNF-α and IL-1β concentrations were analyzed with an enzyme-linked immunosorbent assay. Perfusates were collected during the initial perfusion as well as after 24 hours of preservation and during the reperfusion. Tissue samples were harvested just after animal sacrifice, and after organ perfusion and reperfusion.
  
  
  RESULTS
  A decrease in TNF-α concentration in homogenates was noted after both perfusion and reperfusion in all experimental groups. In contrast, there was a significant decrease in IL-1β concentration in the group with combined human FUT and GAL transgenes. Concurrently, increases in TNF-α and IL-1β concentrations were observed in the reperfusion perfusates in all groups.
  
  
  CONCLUSIONS
  This study shows that IL-1β is synthesized in the ischemic livers of the transgenic animals with both human α1,2-fucosyltransferase and α-galactosidase transgenes. Further analysis is required to determine the importance of this observation.
authors: Department of General, Vascular and Transplant Surgery, Medical University of Silesia, Katowice, Poland.
authors: P
authors: P
authors: Roman
authors: Department of General, Vascular and Transplant Surgery, Medical University of Silesia, Katowice, Poland. Electronic address: gregorydr@wp.pl.
authors: G
authors: G
authors: Budziński
authors: Department of Histology and Embryology, Medical University of Silesia, Katowice, Poland.
authors: A
authors: A
authors: Suszka-Świtek
authors: Department of General, Vascular and Transplant Surgery, Medical University of Silesia, Katowice, Poland.
authors: A
authors: A
authors: Caban
authors: Department of General, Vascular and Transplant Surgery, Medical University of Silesia, Katowice, Poland.
authors: G
authors: G
authors: Oczkowicz
authors: Department of Histology and Embryology, Medical University of Silesia, Katowice, Poland.
authors: R
authors: R
authors: Wiaderkiewicz
authors: The National Research Institute of Animal Production in Kraków-Balice.
authors: F
authors: F
authors: Ryszka
authors: Biochefa Pharmaceutical Research Production Plant, Sosnowiec, Poland.
authors: Z
authors: Z
authors: Smorąg
authors: Department of General, Vascular and Transplant Surgery, Medical University of Silesia, Katowice, Poland.
authors: L
authors: L
authors: Cierpka
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004797:Enzyme-Linked Immunosorbent Assay; D005647:Fucosyltransferases / Q000235:genetics; D018014:Gene Transfer Techniques; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D034421:Sus scrofa; D013552:Swine / Q000235:genetics*; D014183:Transplantation, Heterologous; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D000519:alpha-Galactosidase / Q000235:genetics; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D005647:Fucosyltransferases; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D000519:alpha-Galactosidase; D000097631:Galactoside 2-alpha-L-fucosyltransferase