title: Thymic E4bp4 gene transcription is up-regulated in the chicken during experimental peritonitis modified by the season-related lighting conditions.
abstract: Immunity, like other vertebrate processes, undergoes the diurnal and seasonal rhythmicity generated and synchronized by the endogenous clock. In the present study a transcription of the selected clock genes was evaluated in the chicken thymus to verify its supposed role as a peripheral clock and to check its relation with the seasonality of immune function. Chickens kept from hatch in the season-related lighting conditions (LD 16:8 in summer vs 8:16 in winter) and in a controlled temperature were exposed to the experimental peritonitis elicited by i.p. thioglycollate injection. Previously described seasonality of the inflammatory response has been confirmed and the diurnal rhythms of a core clock gene Per3 and its repressor E4bp4 in the thymus has been evidenced. E4bp4 transcription was up-regulated in inflamed chickens while that of Per3 appeared independent of the locally induced inflammation. Our results suggest an interconnecting role of E4BP4 between molecular clock and immunity in the chicken.
authors: Department of Animal Physiology, Institute of Zoology, Faculty of Biology, University of Warsaw, ul. Miecznikowa 1, 02-096 Warsaw, Poland. Electronic address: eturkowska@biol.uw.edu.pl.
authors: Elzbieta
authors: E
authors: Turkowska
authors: Department of Animal Physiology, Institute of Zoology, Faculty of Biology, University of Warsaw, ul. Miecznikowa 1, 02-096 Warsaw, Poland.
authors: Dominika
authors: D
authors: Pietruszka
authors: Department of Animal Physiology, Institute of Zoology, Faculty of Biology, University of Warsaw, ul. Miecznikowa 1, 02-096 Warsaw, Poland.
authors: Krystyna
authors: K
authors: Skwarlo-Sonta
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D001715:Bird Diseases / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D008027:Light / Q000009:adverse effects; D023421:Models, Animal; D056950:Period Circadian Proteins / Q000235:genetics; D010507:Periodicity; D010538:Peritonitis / Q000139:chemically induced / Q000276:immunology*; D012621:Seasons; D013864:Thioglycolates / Q000008:administration & dosage; D013950:Thymus Gland / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D030161:Avian Proteins; D050976:Basic-Leucine Zipper Transcription Factors; C497441:Nfil3 protein, mouse; D056950:Period Circadian Proteins; D013864:Thioglycolates
keywords: Biological clock; Chicken; E4bp4; Inflammation; Peripheral clock; Thymus