title: Antioxidant and DNA damage protecting potentials of polysaccharide extracted from Phellinus baumii using a delignification method.
abstract: A delignification method was employed to extract the polysaccharide from the fruiting body of Phellinus baumii. The three parameters, processing temperature, ratio of water to raw material and amount of acetic acid every time were optimized using the Box-Behnken design. As a result, the optimal extraction conditions were: processing temperature 70.3°C, ratio of water to raw material of 34.7mL/g and amount of acetic acid of 0.32mL every time. Under these conditions, the highest yield of polysaccharide (10.28%) was obtained. The main fraction (PPB-2) purified from PPB was composed of fucose, arabinose, galactose, glucose, xylose and mannose, while glucose was the predominant monosaccharide. PPB-2 exhibited noticeable antioxidant activity and strong protection against oxidative DNA damage. These findings implied that acid-chlorite delignification was a superior method to extract the polysaccharide from P. baumii and PPB-2 may be useful for cancer chemoprevention.
authors: Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, PR China.
authors: Qun-Li
authors: QL
authors: Jin
authors: Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, PR China. Electronic address: zzf2050@sohu.com.
authors: Zuo-Fa
authors: ZF
authors: Zhang
authors: Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, PR China.
authors: Guo-Ying
authors: GY
authors: Lv
authors: Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, PR China.
authors: Wei-Ming
authors: WM
authors: Cai
authors: Zhejiang Forestry Academy, Hangzhou, PR China.
authors: Jun-Wen
authors: JW
authors: Cheng
authors: Chunan Sangdu Mushroom Specialized Farmers cooperatives, Hangzhou, PR China.
authors: Jian-Gong
authors: JG
authors: Wang
authors: Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, PR China.
authors: Lei-Fa
authors: LF
authors: Fan
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D001487:Basidiomycota / Q000737:chemistry*; D004249:DNA Damage; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification; D010957:Plasmids / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D062610:Fungal Polysaccharides
keywords: Acid-chlorite delignification; Oxidative DNA damage; Phellinus baumill; Polysaccharide