title: Acceleration of wound healing by ultrasound activation of TiO2in Escherichia coli-infected wounds in mice.
abstract: Surgical site infections continue to be a common complication affecting surgical prognosis. Reactive oxygen species (ROS) are generated by ultrasound-irradiated titanium dioxide (TiO2) (UIT). Although excessive ROS production can cause cell damage, ROS at physiological levels mediate beneficial cellular responses, including angiogenesis. This study investigated whether UIT can promote healing of Escherichia coli-infected wounds. We used TiO2and ultrasound irradiation using an ultrasonography machine at a frequency of 1.0 MHz and intensity of 0.4 W cm-2. These levels are not bactericidal in vitro; therefore, we could study the effect of UIT on E. coli survival without interference of ultrasound effects. The number of cluster of differentiation 31-positive blood vessels, which are indicative of angiogenesis, was decreased by bacterial infection, and increased at the wound edges in the UIT-treated infected wounds, suggesting upregulation of neovascularization by UIT. Although UIT treatment did not decrease E. coli survival in vivo, it promoted healing of the infected wounds as evidenced by a significant decrease in the wound area in the UIT-treated mice. Our findings demonstrate that UIT promotes wound healing in surgical site infections and suggest beneficial use of the UIT-based approach as a novel therapeutic method to treat infected surgical wounds. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2344-2351, 2017.
authors: Department of Surgery, Tokyo Medical Center, Tokyo, Japan.
authors: Koji
authors: K
authors: Osumi
authors: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors: Sachiko
authors: S
authors: Matsuda
authors: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors: Naoki
authors: N
authors: Fujimura
authors: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors: Kentaro
authors: K
authors: Matsubara
authors: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors: Minoru
authors: M
authors: Kitago
authors: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors: Osamu
authors: O
authors: Itano
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan.
authors: Chiaki
authors: C
authors: Ogino
authors: Institute of Nature and Environmental Technology, Kanazawa University, Kanazawa, Japan.
authors: Nobuaki
authors: N
authors: Shimizu
authors: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors: Hideaki
authors: H
authors: Obara
authors: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors: Yuko
authors: Y
authors: Kitagawa
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability / Q000187:drug effects; D014025:Titanium / Q000494:pharmacology*; D000069453:Ultrasonic Waves; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: C009495:titanium dioxide; D014025:Titanium
keywords: neovascularization; reactive oxygen species; surgical site infections; titanium dioxide; ultrasound irradiation