title: Conidiobolus macrosporus (Entomophthorales), a mosquito pathogen in Central Brazil.
abstract: A new fungal pathogen of Culicinae (Diptera: Culicidae) adults, Conidiobolus macrosporus (Entomophthorales: Ancylistaceae), was detected and isolated during a survey of mosquito pathogens close to the city of Aruanã, Goiás State, in December 2014. The morphological characteristics of C. macrosporus are presented, and reasons for some uncertainty about this identification are discussed. The pathogenicity and high virulence of this fungus for Aedes aegypti were confirmed in laboratory conditions. Mortality of adults exposed to conidia was observed within 24h of exposure to the pathogen, and increased to 100% as quickly as 3days after inoculation (with the highest conidial concentration tested, 8.3×10(4)conidia/cm(2)). Repeated attempts to obtain genomic sequence data failed despite confirmations that the DNA extraction methods were themselves successful.
authors: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil; Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Casilla 167, Universidad Austral de Chile, Valdivia, Chile. Electronic address: montalva.cristian@gmail.com.
authors: Cristian
authors: C
authors: Montalva
authors: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil; Instituto Federal de Educação, Ciência e Tecnologia de Goiás, Aparecida de Goiânia, Goiás, Brazil. Electronic address: luizfnr@hotmail.com.
authors: Luiz F N
authors: LFN
authors: Rocha
authors: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. Electronic address: evertonkort@yahoo.com.br.
authors: Éverton K K
authors: ÉKK
authors: Fernandes
authors: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. Electronic address: wchrisluz@hotmail.com.
authors: Christian
authors: C
authors: Luz
authors: USDA-ARS Emerging Pests and Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health, Ithaca, NY, USA. Electronic address: richard.humber@usda.gov.
authors: Richard A
authors: RA
authors: Humber
mesh_terms: D000330:Aedes / Q000469:parasitology*; D000818:Animals; D001938:Brazil; D020092:Conidiobolus / Q000235:genetics* / Q000472:pathogenicity*; D009032:Mosquito Control / Q000379:methods; D010572:Pest Control, Biological / Q000379:methods; D014774:Virulence; D020096:Zygomycosis / Q000662:veterinary*
keywords: Aedes aegypti; Entomopathogenic fungus; Entomophthoromycota; Natural enemy