title: Chickpea Ferritin CaFer1 Participates in Oxidative Stress Response, and Promotes Growth and Development.
abstract: Ferritins store and sequester iron, and regulate iron homeostasis. The cDNA for a stress-responsive phytoferritin, previously identified in the extracellular matrix (ECM) of chickpea (Cicer arietinum), was cloned and designated CaFer1. The CaFer1 transcript was strongly induced in chickpea exposed to dehydration, hypersalinity and ABA treatment. Additionally, it has role in the defense against Fusarium oxysporum infection. Functional complementation of the yeast frataxin-deficient mutant, Δyfh1, indicates that CaFer1 functions in oxidative stress. The presence of CaFer1 in the extracellular space besides chloroplast establishes its inimitable nature from that of other phytoferritins. Furthermore, CaFer1 expression in response to iron suggests its differential mechanism of accumulation at two different iron conditions. CaFer1-overexpressing transgenic plants conferred improved growth and development, accompanied by altered expression of iron-responsive genes. Together, these results suggest that the phytoferritin, CaFer1, might play a key role in maintenance of iron buffering and adaptation to environmental challenges.
authors: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors: Shaista
authors: S
authors: Parveen
authors: Department of Biotechnology, TERI University, New Delhi, India.
authors: Deepti Bhushan
authors: DB
authors: Gupta
authors: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors: Suchismita
authors: S
authors: Dass
authors: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors: Amit
authors: A
authors: Kumar
authors: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors: Aarti
authors: A
authors: Pandey
authors: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors: Subhra
authors: S
authors: Chakraborty
authors: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors: Niranjan
authors: N
authors: Chakraborty
mesh_terms: D029863:Cicer / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005110:Extracellular Space; D005293:Ferritins / Q000378:metabolism*; D005670:Fusarium; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D006706:Homeostasis; D007501:Iron / Q000378:metabolism; D033862:Iron-Binding Proteins / Q000235:genetics; D009154:Mutation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified; D036226:Seedlings; D000096923:Frataxin
chemical_list: D018076:DNA, Complementary; D005293:Ferritins; D007501:Iron; D033862:Iron-Binding Proteins; D010940:Plant Proteins; D000096923:Frataxin; C030124:phytoferritin