title: Transcriptomic/proteomic identification of allergens in the mite Tyrophagus putrescentiae.
abstract: While a number of allergens from house dust mites have been described, much remains to be discovered about allergens from storage mites. Here, next-generation sequencing was combined with MS/MS shotgun proteomics to identify proteins, especially potential allergens from Tyrophagus putrescentiae, commonly found in stored food products, especially flour. cDNAs of suspected allergens were cloned and expressed from bacterial cells, and recombinant allergens were tested for binding to IgE in sera from T. putrescentiae-sensitive patients. These analyses identified three previously uncharacterized allergens, Tyr p 28, Tyr p 35, and Tyr p 36, which have been officially assigned by the WHO/IUIS Allergen Nomenclature Sub-committee. Recombinant proteins rTyr p 28, rTyr p 35, and rTyr p 36 bound with 47.1%, 82.4%, and 70.6% of T. putrescentiae-sensitive sera. We provide here a new method to identify allergens by the combination of transcriptomic and proteomic approaches.
authors: Department of Central Laboratory, The Third People's Hospital of Yancheng, Affiliated Yancheng Hospital, School of Medicine, Southeast University, Yancheng, Jiangsu Province, China. ybcui1975@hotmail.com.
authors: Y
authors: Y
authors: Cui
authors: Department of Laboratory Medicine, Yancheng Health Vocational & Technical College, Yancheng, Jiangsu Province, China.
authors: L
authors: L
authors: Yu
authors: Department of Laboratory Medicine, Yancheng Health Vocational & Technical College, Yancheng, Jiangsu Province, China.
authors: F
authors: F
authors: Teng
authors: Department of Laboratory Medicine, Yancheng Health Vocational & Technical College, Yancheng, Jiangsu Province, China.
authors: C
authors: C
authors: Zhang
authors: Department of Laboratory Medicine, Yancheng Health Vocational & Technical College, Yancheng, Jiangsu Province, China.
authors: N
authors: N
authors: Wang
authors: Department of Laboratory Medicine, Yancheng Health Vocational & Technical College, Yancheng, Jiangsu Province, China.
authors: L
authors: L
authors: Yang
authors: Department of Central Laboratory, The Third People's Hospital of Yancheng, Affiliated Yancheng Hospital, School of Medicine, Southeast University, Yancheng, Jiangsu Province, China.
authors: Y
authors: Y
authors: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D000485:Allergens / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics; D059467:Transcriptome; D055815:Young Adult
chemical_list: D000485:Allergens; D020543:Proteome
keywords: Tyrophagus putrescentiae; allergen; proteome; storage mite; transcriptome