title: Overexpression of transport proteins improves the production of 5-aminovalerate from l-lysine in Escherichia coli.
abstract: Bacterial transporters mediate the exchanges between intracellular and extracellular environments. Modification of transport route could be applied to speed up the metabolic reactions and promote the production of aimed compounds. Herein, lysine 2-monooxygenase (DavB) and δ-aminovaleramidase (DavA) were co-expressed in Escherichia coli BL21(DE3) to produce nylon-5 monomer 5-aminovalerate from l-lysine. Then, PP2911 (4-aminobutyrate transporter in Pseudomonas putida) and LysP (the lysine specific permease in E. coli) were overexpressed to promote 5-aminovalerate production using whole cells of recombinant E. coli. The constructed E. coli strain overexpressing transport proteins exhibited good 5-aminovalerate production performance and might serve as a promising biocatalyst for 5-aminovalerate production from l-lysine. This strategy not only shows an efficient process for the production of nylon monomers but also might be used in production of other chemicals.
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors: Zhong
authors: Z
authors: Li
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors: Jing
authors: J
authors: Xu
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors: Tongtong
authors: T
authors: Jiang
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors: Yongsheng
authors: Y
authors: Ge
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors: Pan
authors: P
authors: Liu
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors: Manman
authors: M
authors: Zhang
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors: Zhiguo
authors: Z
authors: Su
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors: Chao
authors: C
authors: Gao
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors: Cuiqing
authors: C
authors: Ma
authors: School of Life Sciences &Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors: Ping
authors: P
authors: Xu
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism; D021542:Amino Acids, Neutral / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D008239:Lysine / Q000378:metabolism*; D060847:Metabolic Engineering; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D016958:Pseudomonas putida / Q000201:enzymology; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D026905:Amino Acid Transport Systems; D021542:Amino Acids, Neutral; D001426:Bacterial Proteins; D002352:Carrier Proteins; D011994:Recombinant Proteins; C013809:5-aminovaleric acid; D006899:Mixed Function Oxygenases; C100031:lysine monooxygenase; D008239:Lysine