title: Pro- and anti-angiogenic VEGF mRNAs in autoimmune thyroid diseases.
abstract: The aim of this study was to assess the relationships between five different splice isoforms of VEGF mRNA and its plasma levels in individuals treated for autoimmune thyroid diseases (AITD); mainly Graves' disease (GD) and Hashimoto's thyroiditis (HT). In a population from Tunisia, levels of thyroid hormones and antibodies were quantified simultaneously with plasma VEGF and VEGF mRNA isoforms after a period of 6 months of patients' treatment. Plasma VEGF was measured in 110 AITD patients (21 GD and 89 HT patients). VEGF isoforms (VEGF121, VEGF165, VEGF145 and VEGF189 pro-angiogenic isoforms and VEGF165b anti-angiogenic isoform) in peripheral blood mononuclear cells were quantified in 71 patients (20 GD and 51 HT patients) and 86 healthy controls. Decreased levels of VEGF189 mRNA were observed in AITD compared to controls. VEGF165 was increased in GD patients compared to controls and the VEGF165b was increased in HT patients compared to GD. We observed increased levels of VEGF165b in hypothyroid AITD patients after treatment. We have also shown that the VEGF145 isoform levels were determined by FT4 in all patients and by the thyroid status after 6 months of treatment only in HT patients. An association was observed for VEGF165 mRNA levels with anti-TPO antibodies in all patients. Finally, FT4 was associated with VEGF plasma levels but only in healthy controls. In conclusion, this descriptive study highlights the specificity of VEGF mRNA isoforms in AITD, a fact underlining the need for novel clinical trials and the development of personalised theranostic approaches.
authors: a UMR INSERM U1122, Interactions Gène-Environnement en Physiopathologie Cardio-Vasculaire (IGE-PCV), Université de Lorraine , Nancy , France.|b Department of Endocrinology , Centre Hospitalier Robert Schuman , Luxembourg, Luxembourg.
authors: Marc
authors: M
authors: Rancier
authors: a UMR INSERM U1122, Interactions Gène-Environnement en Physiopathologie Cardio-Vasculaire (IGE-PCV), Université de Lorraine , Nancy , France.|c Laboratory of Genetics , Biodiversity and Bioresource Valorization, Superior Institute of Biotechnology of Monastir, University of Monastir , Monastir , Tunisia.
authors: Ines
authors: I
authors: Zaaber
authors: a UMR INSERM U1122, Interactions Gène-Environnement en Physiopathologie Cardio-Vasculaire (IGE-PCV), Université de Lorraine , Nancy , France.
authors: Maria G
authors: MG
authors: Stathopoulou
authors: a UMR INSERM U1122, Interactions Gène-Environnement en Physiopathologie Cardio-Vasculaire (IGE-PCV), Université de Lorraine , Nancy , France.|d Department of Endocrinology and Medical Gynecology , CHU Nancy-Brabois , Nancy , France.
authors: Jérôme
authors: J
authors: Chatelin
authors: a UMR INSERM U1122, Interactions Gène-Environnement en Physiopathologie Cardio-Vasculaire (IGE-PCV), Université de Lorraine , Nancy , France.
authors: Abdelsalam
authors: A
authors: Saleh
authors: e Department of Internal Medicine-Endocrinology , Hospital Fattouma Bourguiba in Monastir , Monastir , Tunisia.
authors: Héla
authors: H
authors: Marmouch
authors: a UMR INSERM U1122, Interactions Gène-Environnement en Physiopathologie Cardio-Vasculaire (IGE-PCV), Université de Lorraine , Nancy , France.
authors: Said
authors: S
authors: El Shamieh
authors: a UMR INSERM U1122, Interactions Gène-Environnement en Physiopathologie Cardio-Vasculaire (IGE-PCV), Université de Lorraine , Nancy , France.
authors: Christine
authors: C
authors: Masson
authors: f Randox Laboratories , Crumlin , UK , and.
authors: Helena
authors: H
authors: Murray
authors: f Randox Laboratories , Crumlin , UK , and.
authors: John
authors: J
authors: Lamont
authors: f Randox Laboratories , Crumlin , UK , and.
authors: Peter
authors: P
authors: Fitzgerald
authors: e Department of Internal Medicine-Endocrinology , Hospital Fattouma Bourguiba in Monastir , Monastir , Tunisia.
authors: Silvia
authors: S
authors: Mahjoub
authors: c Laboratory of Genetics , Biodiversity and Bioresource Valorization, Superior Institute of Biotechnology of Monastir, University of Monastir , Monastir , Tunisia.
authors: Khaled
authors: K
authors: Said
authors: c Laboratory of Genetics , Biodiversity and Bioresource Valorization, Superior Institute of Biotechnology of Monastir, University of Monastir , Monastir , Tunisia.
authors: Besma Bel Haj Jrad
authors: BB
authors: Tensaout
authors: c Laboratory of Genetics , Biodiversity and Bioresource Valorization, Superior Institute of Biotechnology of Monastir, University of Monastir , Monastir , Tunisia.
authors: Souhir
authors: S
authors: Mestiri
authors: a UMR INSERM U1122, Interactions Gène-Environnement en Physiopathologie Cardio-Vasculaire (IGE-PCV), Université de Lorraine , Nancy , France.|g Department of Internal Medicine and Geriatrics , CHU Nancy-Brabois , Nancy , France.
authors: Sophie
authors: S
authors: Visvikis-Siest
mesh_terms: D000328:Adult; D017398:Alternative Splicing; D001323:Autoantibodies; D001327:Autoimmune Diseases / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology* / Q000628:therapy; D015415:Biomarkers; D016022:Case-Control Studies; D005260:Female; D006111:Graves Disease / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology* / Q000628:therapy; D006801:Humans; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D042442:Vascular Endothelial Growth Factors / Q000097:blood / Q000235:genetics*
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D012333:RNA, Messenger; D042442:Vascular Endothelial Growth Factors
keywords: Biomarkers; Graves’ disease; Hashimoto thyroiditis; VEGF; splicing isoforms