title: DNA-PkCSexpression in oropharyngeal squamous cell carcinoma: Correlations with human papillomavirus status and recurrence after transoral robotic surgery.
abstract: Human papillomavirus (HPV)-positive oropharyngeal squamous cell carcinoma (SCC) has improved clinical outcomes compared to HPV-negative disease. However, the biology underlying differences in prognosis remains unclear.
  
  
  
  We characterized the expression of DNA-protein kinase catalytic subunit (DNA-PkCS), a key DNA repair protein also associated with tumor progression, in 29 cases of oropharyngeal SCCs and correlated our findings with HPV status and disease recurrence. In addition, we assessed therapeutic response, migration, and invasion in head and neck cancer cell lines upon DNA-PkCSknockdown.
  
  
  
  DNA-PkCSexpression was significantly decreased in HPV-positive compared to HPV-negative oropharyngeal SCC samples. Within the HPV-positive subgroup, DNA-PkCSexpression was inversely related to HPV E6 and E7 expression and trended toward significance as a predictor of recurrence. DNA-PkCSknockdown in cell lines resulted in increased sensitivity to cisplatin and radiotherapy and reduced cell migration and invasion.
  
  
  
  These results suggest DNA-PkCSshould be further studied as a potential marker of tumor progression in HPV-positive oropharyngeal SCCs. © 2016 Wiley Periodicals, Inc. Head Neck 39: 206-214, 2017.
authors: Department of Radiation Oncology, University of Alabama at Birmingham, Birmingham, Alabama.
authors: Alice N
authors: AN
authors: Weaver
authors: Department of Radiation Oncology, University of Alabama at Birmingham, Birmingham, Alabama.
authors: Tiffiny S
authors: TS
authors: Cooper
authors: Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama.
authors: Shi
authors: S
authors: Wei
authors: Department of Otolaryngology - Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama.
authors: William R
authors: WR
authors: Carroll
authors: Department of Otolaryngology - Head and Neck Surgery, Stanford University, Palo Alto, California.
authors: Eben L
authors: EL
authors: Rosenthal
authors: Department of Radiation Oncology, University of Alabama at Birmingham, Birmingham, Alabama.
authors: Eddy S
authors: ES
authors: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D001707:Biopsy, Needle; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000401:mortality / Q000601:surgery / Q000821:virology*; D015331:Cohort Studies; D004279:DNA, Viral / Q000032:analysis; D018572:Disease-Free Survival; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000401:mortality / Q000821:virology; D009959:Oropharyngeal Neoplasms / Q000235:genetics / Q000401:mortality / Q000601:surgery / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D012189:Retrospective Studies; D018570:Risk Assessment; D065287:Robotic Surgical Procedures / Q000379:methods*; D018709:Statistics, Nonparametric; D016019:Survival Analysis; D014481:United States
chemical_list: C109198:CIB1 protein, human; D002135:Calcium-Binding Proteins; D004279:DNA, Viral
keywords: DNA-protein kinase catalytic subunit (DNA-PkCS); human papillomavirus (HPV); oropharyngeal squamous cell carcinoma (SCC); recurrence