title: Capsular polysaccharide from Mycoplasma mycoides subsp. mycoides shows potential for protection against contagious bovine pleuropneumonia.
abstract: Contagious Bovine Pleuropneumonia (CBPP) is a severe respiratory disease caused by Mycoplasma mycoides subsp. mycoides (Mmm) which is widespread in Africa. The capsule polysaccharide (CPS) of Mmm is one of the few identified virulence determinants. In a previous study, immunization of mice against CPS generated antibodies, but they were not able to prevent multiplication of Mmm in this model animal. However, mice cannot be considered as a suitable animal model, as Mmm does not induce pathology in this species. Our aim was to induce antibody responses to CPS in cattle, and challenge them when they had specific CPS antibody titres similar or higher than those from cattle vaccinated with the live vaccine. The CPS was linked to the carrier protein ovalbumin via a carbodiimide-mediated condensation with 1-ethyl-3(3-imethylaminopropyl) carbodiimide (EDC). Ten animals were immunized twice and challenged three weeks after the booster inoculation, and compared to a group of challenged non-immunized cattle. When administered subcutaneously to adult cattle, the vaccine elicited CPS-specific antibody responses with the same or a higher titre than animals vaccinated with the live vaccine. Pathology in the group of immunized animals was significantly reduced (57%) after challenge with Mmm strain Afadé compared to the non-immunized group, a figure in the range of the protection provided by the live vaccine.
authors: Kenya Agricultural and Livestock Research Organization, Biotechnology Research Institute, P.O. Box 14733, 00800, Nairobi, Kenya. Electronic address: martin.mwirigi@kalro.org.
authors: Martin
authors: M
authors: Mwirigi
authors: Kenya Agricultural and Livestock Research Organisation, Veterinary Science Research Institute, P.O. Box 32, 00902, Kikuyu, Kenya.
authors: Isabel
authors: I
authors: Nkando
authors: University of Nairobi, P.O. Box 30197, Nairobi, Kenya.
authors: Moses
authors: M
authors: Olum
authors: Vaccine and Infectious Disease Organization - International Vaccine Centre, University of Saskatchewan, 120 Veterinary Rd., Saskatoon, SK, S7N 5E3, Canada.
authors: Samuel
authors: S
authors: Attah-Poku
authors: University of Nairobi, P.O. Box 30197, Nairobi, Kenya.
authors: Horace
authors: H
authors: Ochanda
authors: Vaccine and Infectious Disease Organization - International Vaccine Centre, University of Saskatchewan, 120 Veterinary Rd., Saskatoon, SK, S7N 5E3, Canada.
authors: Emil
authors: E
authors: Berberov
authors: Vaccine and Infectious Disease Organization - International Vaccine Centre, University of Saskatchewan, 120 Veterinary Rd., Saskatoon, SK, S7N 5E3, Canada.
authors: Andrew
authors: A
authors: Potter
authors: Vaccine and Infectious Disease Organization - International Vaccine Centre, University of Saskatchewan, 120 Veterinary Rd., Saskatoon, SK, S7N 5E3, Canada.
authors: Volker
authors: V
authors: Gerdts
authors: Vaccine and Infectious Disease Organization - International Vaccine Centre, University of Saskatchewan, 120 Veterinary Rd., Saskatoon, SK, S7N 5E3, Canada.
authors: Jose
authors: J
authors: Perez-Casal
authors: Kenya Agricultural and Livestock Research Organisation, Veterinary Science Research Institute, P.O. Box 32, 00902, Kikuyu, Kenya.
authors: Hezron
authors: H
authors: Wesonga
authors: Kenya Agricultural and Livestock Research Organization, Biotechnology Research Institute, P.O. Box 14733, 00800, Nairobi, Kenya.
authors: Reuben
authors: R
authors: Soi
authors: International Livestock Research Institute, P.O. Box 30709, 00100, Nairobi, Kenya.
authors: Jan
authors: J
authors: Naessens
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D016667:Bacterial Capsules / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D007117:Immunization, Secondary / Q000662:veterinary; D051379:Mice; D009176:Mycoplasma mycoides / Q000276:immunology*; D011002:Pleuropneumonia, Contagious / Q000276:immunology / Q000517:prevention & control*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D018074:Vaccines, Conjugate / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D011135:Polysaccharides, Bacterial; D018074:Vaccines, Conjugate
keywords: Capsular polysaccharide; Conjugate vaccine; Contagious bovine pleuropneumonia; Mycoplasma mycoides subsp. mycoides