title: NOX2 Antisense Attenuates Hypoxia-Induced Oxidative Stress and Apoptosis in Cardiomyocyte.
abstract: Heart ischemia is a hypoxia related disease. NOX2 and HIF-1α proteins were increased in cardiomyocytes after acute myocardial infarction. However, the relationship of the hypoxia-induced HIF-1α. NOX2-derived oxidative stress and apoptosis in cardiomyocyte remains unclear. In the current study, we use NOX2 antisense strategy to investigate the role of NOX2 in hypoxia-induced oxidative stress and apoptosis in rat cardiomyocytes. Here, we show that transduction of ADV-NOX2-AS induces potent silencing of NOX2 in cardiomyocytes, and resulting in attenuation of hypoxia-induced oxidative stress and apoptosis. This study indicates the potential of antisense-based therapies and validates NOX2 as a potent therapeutic candidate for heart ischemia.
authors: Department of cardiology, China-Japan union hospital of Jilin University, Changchun, Jilin, 130033, P.R. China.
authors: Bo
authors: B
authors: Yu
authors: Department of cardiology, China-Japan union hospital of Jilin University, Changchun, Jilin, 130033, P.R. China.
authors: Fanbo
authors: F
authors: Meng
authors: Department of cardiology, China-Japan union hospital of Jilin University, Changchun, Jilin, 130033, P.R. China.
authors: Yushuang
authors: Y
authors: Yang
authors: Department of cardiology, China-Japan union hospital of Jilin University, Changchun, Jilin, 130033, P.R. China.
authors: Dongna
authors: D
authors: Liu
authors: Department of cardiology, China-Japan union hospital of Jilin University, Changchun, Jilin, 130033, P.R. China.
authors: Kaiyao
authors: K
authors: Shi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D015687:Cell Hypoxia / Q000235:genetics; D004195:Disease Models, Animal; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D009203:Myocardial Infarction / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D015428:Myocardial Reperfusion Injury / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000235:genetics*; D016376:Oligonucleotides, Antisense / Q000302:isolation & purification* / Q000378:metabolism / Q000627:therapeutic use; D018384:Oxidative Stress / Q000235:genetics; D051381:Rats
chemical_list: C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008562:Membrane Glycoproteins; D016376:Oligonucleotides, Antisense; C500593:Cybb protein, rat; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases
keywords: Heart infarction; Heart ischemia; NOX2; Oxidative stress; and apoptosis