title: Electron acceptor redox potential globally regulates transcriptomic profiling in Shewanella decolorationis S12.
abstract: Electron acceptor redox potential (EARP) was presumed to be a determining factor for microbial metabolism in many natural and engineered processes. However, little is known about the potentially global effects of EARP on bacteria. In this study, we compared the physiological and transcriptomic properties of Shewanella decolorationis S12 respiring with different EARPs in microbial electrochemical systems to avoid the effects caused by the other physicochemical properties of real electron acceptor. Results showed that the metabolic activities of strain S12 were nonlinear responses to EARP. The tricarboxylic acid cycle for central carbon metabolism was down-regulated while glyoxylate shunt was up-regulated at 0.8 V compared to 0.2 and -0.2 V, which suggested that EARP is an important but not the only determinant for metabolic pathways of strain S12. Moreover, few cytochrome c genes were differentially expressed at different EARPs. The energy intensive flagella assembly and assimilatory sulfur metabolism pathways were significantly enriched at 0.8 V, which suggested strain S12 had stronger electrokinesis behavior and oxidative stress-response at high EARP. This study provides the first global information of EARP regulations on microbial metabolism, which will be helpful for understanding microorganism respiration.
authors: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.|Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China.|State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, China.
authors: Yingli
authors: Y
authors: Lian
authors: Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China.|State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, China.
authors: Yonggang
authors: Y
authors: Yang
authors: Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China.|State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, China.
authors: Jun
authors: J
authors: Guo
authors: Science and Technology Library of Guangdong Province, Guangzhou 510070, China.
authors: Yan
authors: Y
authors: Wang
authors: Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China.|State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, China.
authors: Xiaojing
authors: X
authors: Li
authors: Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China.|State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, China.
authors: Yun
authors: Y
authors: Fang
authors: Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China.|State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, China.
authors: Lixia
authors: L
authors: Gan
authors: Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China.|State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, China.
authors: Meiying
authors: M
authors: Xu
mesh_terms: D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D010084:Oxidation-Reduction; D020592:Shewanella / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome / Q000502:physiology*