title: Enzymatic potential of heterotrophic bacteria from a neutral copper mine drainage.
abstract: Copper mine drainages are restricted environments that have been overlooked as sources of new biocatalysts for bioremediation and organic syntheses. Therefore, this study aimed to determine the enzymatic activities (esterase, epoxide hydrolase and monooxygenase) of 56 heterotrophic bacteria isolated from a neutral copper mine drainage (Sossego Mine, Canaã dos Carajás, Brazil). Hydrolase and monooxygenase activities were detected in 75% and 20% of the evaluated bacteria, respectively. Bacterial strains with good oxidative performance were also evaluated for biotransformation of organic sulfides. Fourteen strains with good enzymatic activity were identified by 16S rRNA gene sequencing, revealing the presence of three genera: Bacillus, Pseudomonas and Stenotrophomonas. The bacterial strains B. megaterium (SO5-4 and SO6-2) and Pseudomonas sp. (SO5-9) efficiently oxidized three different organic sulfides to their corresponding sulfoxides. In conclusion, this study revealed that neutral copper mine drainages are a promising source of biocatalysts for ester hydrolysis and sulfide oxidation/bioremediation. Furthermore, this is a novel biotechnological overview of the heterotrophic bacteria from a copper mine drainage, and this report may support further microbiological monitoring of this type of mine environment.
authors: Universidade Estadual de Campinas, Instituto de Química, Campinas, SP, Brazil.
authors: Bruna Zucoloto da
authors: BZ
authors: Costa
authors: Universidade Estadual de Campinas, Centro de Biologia Molecular e Engenharia Genética, Campinas, SP, Brazil.
authors: Viviane Drumond
authors: VD
authors: Rodrigues
authors: Universidade Estadual de Campinas, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas, Campinas, Brazil.
authors: Valéria Maia de
authors: VM
authors: Oliveira
authors: Universidade Estadual de Campinas, Centro de Biologia Molecular e Engenharia Genética, Campinas, SP, Brazil.
authors: Laura Maria Mariscal
authors: LM
authors: Ottoboni
authors: Universidade Estadual de Campinas, Instituto de Química, Campinas, SP, Brazil. Electronic address: anita@iqm.unicamp.br.
authors: Anita Jocelyne
authors: AJ
authors: Marsaioli
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D003300:Copper; D004783:Environmental Microbiology; D004798:Enzymes; D004950:Esterases / Q000235:genetics / Q000378:metabolism; D008906:Mining; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013440:Sulfides / Q000378:metabolism
chemical_list: D004798:Enzymes; D012336:RNA, Ribosomal, 16S; D013440:Sulfides; D003300:Copper; D006899:Mixed Function Oxygenases; D004950:Esterases
keywords: Copper mine drainage; High-throughput screening; Hydrolases; Monooxygenases