title: Characterization and functional studies of fowl adenovirus 9 dUTPase.
abstract: Deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase), a ubiquitous enzyme that catalyzes the hydrolysis of dUTP to dUMP and found in many viruses, has yet to be identified in fowl adenovirus 9 (FAdV-9). By a multiple alignment of dUTPase amino acid sequences, FAdV-9 ORF1 contained the five conserved motifs that define the protein family, and encoded a functional dUTPase. Moreover, transcription and protein expression patterns were characterized, indicating that dUTPase was transcribed from 2h post-infection (h.p.i.) and translated from 6h.p.i., and both continued to the late phase of virus infection. An HA-tagged dUTPase recombinant virus was generated, and dUTPase was found to be localized in both the cytoplasm and nucleus in chicken hepatoma cells (CH-SAH). A dUTPase knockout virus was generated and compared with the wild-type virus, showing that dUTPase upregulated the expression of type I interferons, but was not required for viral DNA or virus replication in CH-SAH cells.
authors: Department of Pathobiology, University of Guelph, Guelph, Canada.
authors: Li
authors: L
authors: Deng
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
authors: Xiaobing
authors: X
authors: Qin
authors: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada.
authors: Peter
authors: P
authors: Krell
authors: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada.
authors: Ray
authors: R
authors: Lu
authors: Department of Pathobiology, University of Guelph, Guelph, Canada.
authors: Shayan
authors: S
authors: Sharif
authors: Department of Pathobiology, University of Guelph, Guelph, Canada. Electronic address: enagy@uoguelph.ca.
authors: Éva
authors: É
authors: Nagy
mesh_terms: D000256:Adenoviridae / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D016207:Cytokines / Q000235:genetics; D004789:Enzyme Activation; D016679:Genome, Viral; D009154:Mutation; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D021381:Protein Transport; D011755:Pyrophosphatases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase
keywords: DUTPase; Fowl adenovirus 9; Type I interferon; Virus replication