title: The Effects of High Temperature on Infection by Potato virus Y, Potato virus A, and Potato leafroll virus.
abstract: We examined the effects of temperature on acquisition of Potato virus Y-O (PVY-O), Potato virus A (PVA), and Potato leafroll virus (PLRV) by Myzus persicae by performing transmission tests with aphids that acquired each virus at different temperatures. Infection by PVY-O/PVA and PLRV increased with increasing plant temperature in Nicotiana benthamiana and Physalis floridana, respectively, after being transmitted by aphids that acquired them within a temperature range of 10-20°C. However, infection rates subsequently decreased. Direct qRT-PCR of RNA extracted from a single aphid showed that PLRV infection increased in the 10-20°C range, but this trend also declined shortly thereafter. We examined the effect of temperature on establishment of virus infection. The greatest number of plants became infected when N. benthamiana was held at 20°C after inoculation with PVY-O or PVA. The largest number of P. floridana plants became infected with PLRV when the plants were maintained at 25°C. PLRV levels were highest in P. floridana kept at 20-25°C. These results indicate that the optimum temperatures for proliferation of PVY-O/PVA and PLRV differed. Western blot analysis showed that accumulations of PVY-O and PVA coat proteins (CPs) were lower at 10°C or 15°C than at 20°C during early infection. However, accumulation increased over time. At 25°C or 30°C, the CPs of both viruses accumulated during early infection but disappeared as time passed. Our results suggest that symptom attenuation and reduction of PVY-O and PVA CP accumulation at higher temperatures appear to be attributable to increased RNA silencing.
authors: National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, Korea.
authors: Bong Nam
authors: BN
authors: Chung
authors: Centro de Investigaciones Biológicas, Centro de Investigaciones BiolÓgicas-Spanish National Research Council, Madrid 28040, Spain.
authors: Tomas
authors: T
authors: Canto
authors: Centro de Investigaciones Biológicas, Centro de Investigaciones BiolÓgicas-Spanish National Research Council, Madrid 28040, Spain.
authors: Francisco
authors: F
authors: Tenllado
authors: National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, Korea.
authors: Kyung San
authors: KS
authors: Choi
authors: National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, Korea.
authors: Jae Ho
authors: JH
authors: Joa
authors: National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, Korea.
authors: Jeong Joon
authors: JJ
authors: Ahn
authors: National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, Korea.
authors: Chun Hwan
authors: CH
authors: Kim
authors: National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, Korea.
authors: Ki Seck
authors: KS
authors: Do
keywords: Potato leafroll virus; Potato virus A, Potato virus Y-O; infection; temperature