title: Optical Nano-mapping and Analysis of Plant Genomes.
abstract: Application of optical mapping based on BioNano Genomics Irys(®) technology ( http://www.bionanogenomics.com/ ) is growing rapidly since its debut in November 2012. The technology can be used to facilitate genome sequence assembly and analysis of genome structural variations. We describe here the detailed protocol that we used to generate a whole genome BioNano map for Aegilops tauschii, the D genome progenitor of hexaploid wheat (Triticum aestivum). We are using the whole genome BioNano map to validate sequence assembly based on the next-generation sequencing, order sequence scaffolds, and ultimately build pseudomolecules for the genome.
authors: Department of Plant Sciences, University of California, One Shields Ave., Davis, CA, 95616, USA. mcluo@ucdavis.edu.
authors: Ming-Cheng
authors: MC
authors: Luo
authors: Department of Plant Sciences, University of California, One Shields Ave., Davis, CA, 95616, USA.
authors: Karin R
authors: KR
authors: Deal
authors: Department of Plant Sciences, University of California, One Shields Ave., Davis, CA, 95616, USA.
authors: Armond
authors: A
authors: Murray
authors: Department of Plant Sciences, University of California, One Shields Ave., Davis, CA, 95616, USA.
authors: Tingting
authors: T
authors: Zhu
authors: BioNano Genomics, 9640 Towne Centre Drive, Ste. 100, San Diego, CA, 92121, USA.
authors: Alex R
authors: AR
authors: Hastie
authors: BioNano Genomics, 9640 Towne Centre Drive, Ste. 100, San Diego, CA, 92121, USA.
authors: Will
authors: W
authors: Stedman
authors: BioNano Genomics, 9640 Towne Centre Drive, Ste. 100, San Diego, CA, 92121, USA.
authors: Henry
authors: H
authors: Sadowski
authors: BioNano Genomics, 9640 Towne Centre Drive, Ste. 100, San Diego, CA, 92121, USA.
authors: Michael
authors: M
authors: Saghbini
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D018745:Genome, Plant; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D055095:Optics and Photonics; D017422:Sequence Analysis, DNA / Q000379:methods*; D014908:Triticum / Q000235:genetics*
keywords: BioNano genome map; Optical mapping; Physical map