title: SRCIN1 Suppressed Osteosarcoma Cell Proliferation and Invasion.
abstract: SRCIN1 (SRC kinase signalling inhibitor 1) is a new tumor suppressor gene. Previous studies showed that SRCIN1 played a tumor suppressor role in the development of lung cancer and breast cancer. However, the role of SRCIN1 in osteosarcoma is still unknown. In this study, we demonstrated that SRCIN1 was downregulated in osteosarcoma cell lines compared with osteoblastic cell line. Moreover, SRCIN1 was downregulated in osteosarcoma tissues compared with the adjacent tissues. Further investigation revealed that overexpression of SRCIN1 inhibited the osteosarcoma cell line MG-63 proliferation. This effect was confirmed by measuring the ki-67 and PCNA expression. SRCIN1 overexpression promoted E-cadherin expression and suppressed N-cadherin, Vimentin and Snail expression, suggesting that SRCIN1 overexpression inhibited EMT of the osteosarcoma cell. In addition, ectopic expression of SRCIN1 inhibited the MG-63 cell colony formation and invasion. These data suggested that SRCIN1 acted as a tumor suppressor gene in the development of osteosarcoma.
authors: Department of Orthopedics, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, China.
authors: Peng
authors: P
authors: Wang
authors: Department of Orthopedics, First Affiliated Hospital of Shantou University Medical College, Shantou, 515041, Guangdong Province, China.
authors: Hu
authors: H
authors: Wang
authors: Department of Orthopedics, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang, 154000, China.
authors: Xiaotao
authors: X
authors: Li
authors: Clinical medicine Grade Four, Harbin Medical University, Harbin, Heilongjiang, 150001, China.
authors: Ying
authors: Y
authors: Liu
authors: Department of Orthopedics, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, China.
authors: Chengbin
authors: C
authors: Zhao
authors: Department of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical University Daqing, Daqing, 163319, China.|Biopharmaceutical Key Laboratory of Heilongjiang Province, Harbin, 150081, China.
authors: Daling
authors: D
authors: Zhu
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism*; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C532615:SRCIN1 protein, human