title: Current methods for preimplantation genetic diagnosis.
abstract: Preimplantation Genetic Diagnosis (PGD) used in assisted reproduction techniques is designed to provide help for couples trying to conceive a child, as it helps deliver healthy offspring. After in vitro fertilization, material is collected from the oocyte (polar body), 3-day-old embryo, or increasingly often, from the trophectoderm of a blastocyst. Selection of the diagnostic method depends on the testing center, but methods such as aCGH (Comparative Genomic Hybridization Array) and NGS (Next-Generation Sequencing) are supposed to have the highest reliability and precision. This paper presents a review of the most important methods used in PGD, their advantages and disadvantages as well as efficacy in the procedures in which they are used.
authors: INVICTA Fertility and Reproductive Centre, Gdansk, Poland. joanna.liss@invicta.pl.
authors: Joanna
authors: J
authors: Liss
authors: Iwona
authors: I
authors: Chromik
authors: Joanna
authors: J
authors: Szczyglińska
authors: Monika
authors: M
authors: Jagiełło
authors: Aron
authors: A
authors: Łukaszuk
authors: Krzysztof
authors: K
authors: Łukaszuk
mesh_terms: D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods / Q000639:trends; D016896:Treatment Outcome
keywords: PGD; PGS; embryo biopsy; in vitro fertilization; next generation sequencing