title: Exosome cargo reflects TGF-β1-mediated epithelial-to-mesenchymal transition (EMT) status in A549 human lung adenocarcinoma cells.
abstract: It has been suggested that tumor cells secrete exosomes to modify the local microenvironment, which then promotes intercellular communication and metastasis. Although exosomes derived from cancer cells may contribute to the epithelial-mesenchymal transition (EMT) in untransformed cells, few studies have defined exosome cargo upon induction of EMT. In this study, we investigated the changes in exosomal cargo from the epithelial to mesenchymal cell phenotype by inducing EMT with transforming growth factor (TGF)-β1 in A549 human lung adenocarcinoma cells. The protein content of the exosomes reflects the change in the cell phenotype. In addition, miR-23a was significantly enriched in the exosomes after mesenchymal transition. Following treatment of exosomes from mesenchymal cells via EMT induction with TGF-β1 to the epithelial cell type, phenotypic changes in protein expression level and cell morphology were observed. Autologous treatment of exosomes enhanced the transcriptional activity and abundance of β-catenin. Our results suggest that the exosomal protein and miRNA content reflects the physiological condition of its source and that exosomes induce phenotypic changes via autocrine signaling.
authors: Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Daejeon, Republic of Korea.
authors: Jiyeon
authors: J
authors: Kim
authors: Department of Pharmacology, School of Medicine, Eulji University, Daejeon, Republic of Korea.
authors: Tae Yeon
authors: TY
authors: Kim
authors: Department of Microbiology, School of Medicine, Eulji University, Daejeon, Republic of Korea.
authors: Myung Shin
authors: MS
authors: Lee
authors: Department of Biomedical Laboratory Science, College of Health Sciences, Eulji University, Seongnam-Si, Gyeonggi-Do, Republic of Korea.
authors: Ji Young
authors: JY
authors: Mun
authors: Department of Laboratory Medicine, School of Medicine, Eulji University, Daejeon, Republic of Korea.
authors: Chunhwa
authors: C
authors: Ihm
authors: Department of Pharmacology, School of Medicine, Eulji University, Daejeon, Republic of Korea. Electronic address: sakim@eulji.ac.kr.
authors: Soon Ae
authors: SA
authors: Kim
mesh_terms: D000072283:A549 Cells; D019898:Autocrine Communication; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects* / Q000235:genetics; D055354:Exosomes / Q000187:drug effects* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D051176:beta Catenin / Q000235:genetics* / Q000378:metabolism
chemical_list: C495270:CTNNB1 protein, human; C552430:MIRN23a microRNA, human; D035683:MicroRNAs; D053773:Transforming Growth Factor beta1; D051176:beta Catenin
keywords: Epithelial to mesenchymal transition; Exosome; Lung adenocarcinoma; MicroRNA; Transforming growth factor β1