title: Expression of microRNAs in fibroblast of pterygium.
abstract: OBJECTIVE
  To screen microRNAs (miRNAs) and set up target miRNAs in pterygium.
  
  
  METHODS
  Primary fibroblasts were isolated from pterygium and Tenon's capsule and cultured. Immunocytochemical analysis and Western blotting were performed to confirm the culture of fibroblasts. In all, 1733 miRNAs were screened in the first step by using GeneChip(®) miRNA3.0 Array. Specific miRNAs involved in the pathogenesis of pterygium were subsequently determined using the following criteria: 1) high reproducibility in a repetitive test; 2) base log value of >7.0 for both control and pterygial fibroblasts; and 3) log ratio of >1.0 between pterygial fibroblasts and control fibroblasts.
  
  
  RESULTS
  Primary screening showed that 887/1733 miRNAs were up-regulated and 846/1733 miRNAs were down-regulated in pterygial fibroblasts compared with those in control fibroblasts. Of the 1733 miRNAs screened, 4 miRNAs, namely, miRNA-143a-3p, miRNA-181a-2-3p, miRNA-377-5p and miRNA-411a-5p, met the above-mentioned criteria. Primary screening showed that these 4 miRNAs were up-regulated in pterygial fibroblasts compared with control fibroblasts and that miRNA-143a-3p had the highest mean ratio compared with the miRNAs in control fibroblasts.
  
  
  CONCLUSIONS
  miRNA-143a-3p, miRNA-181a-2-3p, miRNA-377-5p and miRNA-411a-5p are up-regulated in pterygial fibroblasts compared with control fibroblasts, suggesting their involvement in the pathogenesis of pterygium.
authors: Myung-Gok Eye Research Institute, Konyang University College of Medicine, Seoul 07301, Korea.
authors: Joon H
authors: JH
authors: Lee
authors: Myung-Gok Eye Research Institute, Konyang University College of Medicine, Seoul 07301, Korea.
authors: Sun-Ah
authors: SA
authors: Jung
authors: Department of Ophthalmology, Kim's Eye Hospital, Seoul 07301, Korea.
authors: Young-A
authors: YA
authors: Kwon
authors: Department of Ophthalmology, Kim's Eye Hospital, Seoul 07301, Korea.
authors: Jae-Lim
authors: JL
authors: Chung
authors: Department of Ophthalmology, Kim's Eye Hospital, Seoul 07301, Korea; Department of Ophthalmology, Konyang University College of Medicine, Daejeon 35356, Korea.
authors: Ungsoo Samuel
authors: US
authors: Kim
keywords: fibroblast; microRNA; pterygium