title: Oral exposure to environmental pollutant benzo[a]pyrene impacts the intestinal epithelium and induces gut microbial shifts in murine model.
abstract: Gut microbiota dysbiosis are associated with a wide range of human diseases, including inflammatory bowel diseases. The physiopathology of these diseases has multifactorial aetiology in which environmental factors, particularly pollution could play a crucial role. Among the different pollutants listed, Polycyclic Aromatic Hydrocarbons (PAHs) are subject to increased monitoring due to their wide distribution and high toxicity on Humans. Here, we used 16S rRNA gene sequencing to investigate the impact of benzo[a]pyrene (BaP, most toxic PAH) oral exposure on the faecal and intestinal mucosa-associated bacteria in C57BL/6 mice. Intestinal inflammation was also evaluated by histological observations. BaP oral exposure significantly altered the composition and the abundance of the gut microbiota and led to moderate inflammation in ileal and colonic mucosa. More severe lesions were observed in ileal segment. Shifts in gut microbiota associated with moderate inflammatory signs in intestinal mucosa would suggest the establishment of a pro-inflammatory intestinal environment following BaP oral exposure. Therefore, under conditions of genetic susceptibility and in association with other environmental factors, exposure to this pollutant could trigger and/or accelerate the development of inflammatory pathologies.
authors: Clermont Université, Université d'Auvergne, EA 4678 CIDAM, F-63001 Clermont-Ferrand, France.
authors: Céline
authors: C
authors: Ribière
authors: Clermont Université, Université d'Auvergne, EA 4678 CIDAM, F-63001 Clermont-Ferrand, France.
authors: Pierre
authors: P
authors: Peyret
authors: Clermont Université, Université d'Auvergne, EA 4678 CIDAM, F-63001 Clermont-Ferrand, France.
authors: Nicolas
authors: N
authors: Parisot
authors: Service d'anatomo-pathologie, CHU Clermont-Ferrand, Clermont-Ferrand, France.
authors: Claude
authors: C
authors: Darcha
authors: Service d'anatomo-pathologie, CHU Clermont-Ferrand, Clermont-Ferrand, France.|Clermont Université, Université d'Auvergne, EA 7281 R2D2, F-63001 Clermont-Ferrand, France.
authors: Pierre J
authors: PJ
authors: Déchelotte
authors: Clermont Université, UMR 1071 M2ISH, Inserm/Université d'Auvergne, F-63001 Clermont-Ferrand, France.|INRA, Unité Sous Contrat 2018, F-63000, Clermont-Ferrand, France.
authors: Nicolas
authors: N
authors: Barnich
authors: Clermont Université, Université d'Auvergne, EA 4678 CIDAM, F-63001 Clermont-Ferrand, France.
authors: Eric
authors: E
authors: Peyretaillade
authors: Clermont Université, Université d'Auvergne, EA 4678 CIDAM, F-63001 Clermont-Ferrand, France.|Clermont Université, UMR 1071 M2ISH, Inserm/Université d'Auvergne, F-63001 Clermont-Ferrand, France.|INRA, Unité Sous Contrat 2018, F-63000, Clermont-Ferrand, France.
authors: Delphine
authors: D
authors: Boucher
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001564:Benzo(a)pyrene / Q000008:administration & dosage / Q000633:toxicity*; D004195:Disease Models, Animal; D064806:Dysbiosis / Q000209:etiology / Q000382:microbiology; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000633:toxicity*; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000209:etiology / Q000382:microbiology / Q000473:pathology; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D004785:Environmental Pollutants; D001564:Benzo(a)pyrene