title: Determination of albumin adducts of 4,4'-methylenediphenyl diisocyanate after specific inhalative challenge tests in workers.
abstract: 4,4'-Methylenediphenyl diisocyanate (MDI) is the most important isocyanate used in the industry. Lung sensitization with bronchial asthma is the main disorder in exposed workers. Albumin adducts of MDI might be involved in specific immunological reactions. MDI adducts with lysine (MDI-Lys) of albumin have been found in MDI-workers and construction workers. MDI-Lys is an isocyanate-specific adduct of MDI with albumin. In the present study, we report MDI-adducts in workers undergoing diagnostic MDI challenge tests. The workers were exposed for 2h to 5ppb of MDI. The adduct levels increase significantly after the exposure to MDI in the challenge chamber. About 0.6% of the dose was bound to albumin. So far, only urinary metabolites of MDI were measured to monitor isocyanate workers. However, such urinary metabolites are not isocyanate specific. Therefore, we propose to measure albumin adducts for monitoring MDI exposed subjects.
authors: Institute of Environmental and Occupational Toxicology, Casella Postale 108, CH-6780 Airolo, Switzerland; Walther-Straub-Institut für Pharmakologie und Toxikologie, Ludwig-Maximilians-Universität, D-80336 München, Germany; Department of Environmental Health Sciences, School of Public Health and Tropical Medicine, Tulane University, 1440 Canal St., Suite 2100, New Orleans, LA 70112, USA. Electronic address: gabriele.sabbioni@bluewin.ch.
authors: Gabriele
authors: G
authors: Sabbioni
authors: Department of Environmental Health Sciences, School of Public Health and Tropical Medicine, Tulane University, 1440 Canal St., Suite 2100, New Orleans, LA 70112, USA; National Laboratories Inc., 3011 W. Grand Blvd, Suite 310, Detroit, MI 48202, USA.
authors: Nagaraju
authors: N
authors: Dongari
authors: Department of Environmental Health Sciences, School of Public Health and Tropical Medicine, Tulane University, 1440 Canal St., Suite 2100, New Orleans, LA 70112, USA.
authors: Anoop
authors: A
authors: Kumar
authors: Institut für Arbeitsmedizin, Charité Universitätsmedizin Berlin, Thielallee 69, 14195 Berlin, Germany.
authors: Xaver
authors: X
authors: Baur
mesh_terms: D000328:Adult; D000485:Allergens / Q000097:blood / Q000737:chemistry* / Q000633:toxicity; D059366:Asthma, Occupational / Q000097:blood* / Q000175:diagnosis; D015415:Biomarkers / Q000097:blood; D001985:Bronchial Provocation Tests; D002851:Chromatography, High Pressure Liquid; D042241:Early Diagnosis; D006801:Humans; D017953:Isocyanates / Q000097:blood / Q000737:chemistry* / Q000633:toxicity; D057230:Limit of Detection; D008239:Lysine / Q000032:analysis / Q000097:blood / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D015166:Monitoring, Immunologic; D016273:Occupational Exposure / Q000009:adverse effects; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis / Q000737:chemistry*; D000075462:Serum Albumin, Human; D021241:Spectrometry, Mass, Electrospray Ionization; D013557:Switzerland; D053719:Tandem Mass Spectrometry; D001559:Benzhydryl Compounds
chemical_list: D000485:Allergens; D015415:Biomarkers; D017953:Isocyanates; D008239:Lysine; D012709:Serum Albumin; D000075462:Serum Albumin, Human; C422455:ALB protein, human; C005969:4,4'-diphenylmethane diisocyanate; D001559:Benzhydryl Compounds
keywords: Albumin adducts; Biomonitoring; Isocyanates; Occupational asthma