title: Recovery of Lemna minor after exposure to sulfadimethoxine irradiated and non-irradiated in a solar simulator.
abstract: Sulfonamides are the second most widely used group of veterinary antibiotics which are often detected in the environment. They are eliminated from freshwaters mainly through photochemical degradation. The toxicity of sulfadimethoxine (SDM) was evaluated with the use of Lemna minor before and after 1- and 4-h irradiation in a SunTest CPS+ solar simulator. Eight endpoints consisting of: number and total area of fronds, fresh weight, chlorophylls a and b, carotenoids, activity of catalase and guaiacol peroxidase, and protein content were determined. The total frond area and chlorophyll b content were the most sensitive endpoints with EC50 of 478 and 554 μg  L-1, respectively. The activity of guaiacol peroxidase and catalase increased at SDM concentrations higher than 125 and 500 μg  L-1, respectively. The SDM photodegradation rate for first order kinetics and the half-life were 0.259 h-1and 2.67  h, respectively. The results show that the toxicity of irradiated solutions was caused by SDM only, and the photoproducts appeared to be either non-toxic or much less toxic to L. minor than the parent compound. To study the recovery potential of L. minor, after 7 days exposure in SDM solutions, the plants were transferred to fresh medium and incubated for the next 7 days. L. minor has the ability to regenerate, but a 7-day recovery phase is not sufficient for it to return to an optimal physiological state.
authors: Department of Environmental Health Sciences, Medical University of Warsaw, 1 Banacha Str., 02-097, Warsaw, Poland. agata.drobniewska@wum.edu.pl.
authors: Agata
authors: A
authors: Drobniewska
authors: Department of Environmental Health Sciences, Medical University of Warsaw, 1 Banacha Str., 02-097, Warsaw, Poland.
authors: Dorota
authors: D
authors: Wójcik
authors: Department of Environmental Health Sciences, Medical University of Warsaw, 1 Banacha Str., 02-097, Warsaw, Poland.
authors: Monika
authors: M
authors: Kapłan
authors: Department of Air Protection and Environmental Toxicology, University of Warmia and Mazury in Olsztyn, 17 Prawocheńskiego Str., 10-726, Olsztyn, Poland.
authors: Barbara
authors: B
authors: Adomas
authors: Department of Plant Physiology and Biotechnology, University of Warmia and Mazury in Olsztyn, 1A Oczapowskiego Str., 10-718, Olsztyn, Poland.
authors: Agnieszka
authors: A
authors: Piotrowicz-Cieślak
authors: Department of Environmental Health Sciences, Medical University of Warsaw, 1 Banacha Str., 02-097, Warsaw, Poland.
authors: Grzegorz
authors: G
authors: Nałęcz-Jawecki
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000633:toxicity*; D029064:Araceae / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D001673:Biodegradation, Environmental; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D013412:Sulfadimethoxine / Q000378:metabolism / Q000633:toxicity*
chemical_list: D000900:Anti-Bacterial Agents; D002734:Chlorophyll; D013412:Sulfadimethoxine; D002338:Carotenoids; C037184:chlorophyll b; C121048:guaiacol peroxidase; D009195:Peroxidase
keywords: Duckweed; Photodegradation; Phototoxicity; Phytotoxicity; Recovery assay; Sulfonamides