title: Quantitation of Human Cells that Produce Neutrophils and Platelets in Vivo Obtained from Normal Donors Treated with Granulocyte Colony-Stimulating Factor and/or Plerixafor.
abstract: Plerixafor (P) together with granulocyte colony-stimulating factor (G) is now recognized as an important strategy for mobilizing hematopoietic cells for use in patients given myelosuppressive therapies. However, quantitative comparisons of their ability to mobilize human cells with different hematopoietic activities in vitro or in vivo (in immunodeficient mice) and their interrelationships have not been investigated. To address these questions, we collected samples from 5 normal adult volunteers before and after administering P alone and from another 5 before and after a 4-day course of G and again after a subsequent injection of P. Measurements of their blood content of CD34+cells, in vitro myeloid colony-forming cells, 3- and 6-week long-term culture (LTC) cell outputs, and levels of circulating human platelets, as well as myeloid and lymphoid cells obtained in immunodeficient mice that received transplants, showed all activities were maximal 4 hours after P preceded by G, and 3-week LTC outputs showed the highest concordance with the 3-week circulating human neutrophil levels obtained in mice that received transplants. Thus, human cells capable of producing neutrophils rapidly in vivo were optimally mobilized by the G + P protocol, and the 3-week LTC assay appears to offer a more specific predictor of their levels than conventional CD34+cell or colony-forming cell counts.
authors: Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada; Department of Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
authors: Paul H
authors: PH
authors: Miller
authors: Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada.
authors: Naoto
authors: N
authors: Nakamichi
authors: Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada; Department of Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
authors: David J H F
authors: DJHF
authors: Knapp
authors: Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada.
authors: Gabrielle
authors: G
authors: Rabu
authors: Department of Pediatrics, University of British Columbia, Vancouver, British Columbia, Canada; Childhood Cancer & Blood Research, Child and Family Research Institute, Vancouver, British Columbia, Canada.
authors: Kirk R
authors: KR
authors: Schultz
authors: Department of Medicine, Memorial University, St Johns, Newfoundland.
authors: David M
authors: DM
authors: Jones
authors: Department of Medicine, Dalhousie University, Halifax, Nova Scotia.
authors: Stephen
authors: S
authors: Couban
authors: Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada; Department of Medicine, University of British Columbia, Vancouver, British Columbia, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada. Electronic address: ceaves@bccrc.ca.
authors: Connie J
authors: CJ
authors: Eaves
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000097:blood / Q000187:drug effects; D001596:Benzylamines; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects; D002452:Cell Count; D018929:Cell Culture Techniques; D000080027:Cyclams; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006571:Heterocyclic Compounds / Q000008:administration & dosage*; D064593:Heterografts; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects
chemical_list: D018952:Antigens, CD34; D001596:Benzylamines; D000080027:Cyclams; D006571:Heterocyclic Compounds; D016179:Granulocyte Colony-Stimulating Factor; C088327:plerixafor
keywords: Granulocyte colony–stimulating factor (G-CSF); Immunodeficient mice; Mobilization; Plerixafor