title: Antibody repertoire development in fetal and neonatal piglets. XXIV. Hypothesis: The ileal Peyer patches (IPP) are the major source of primary, undiversified IgA antibodies in newborn piglets.
abstract: The ileal Peyers patches (IPP) of newborn germfree (GF) piglets were isolated into blind loops and the piglets colonized with a defined probiotic microflora. After 5 weeks, IgA levels in the intestinal lavage (IL) of loop piglets remained at GF levels and IgM comprised ∼70% while in controls, IgA levels were elevated 5-fold and comprised ∼70% of total Igs. Loop piglets also had reduced serum IgA levels suggesting the source of serum IgA had been interrupted. The isotype profile for loop contents was intermediate between that in the IL of GF and probiotic controls. Surprisingly, colonization alone did not result in repertoire diversification in the IPP. Rather, colonization promoted pronounced proliferation of fully switched IgA(+)IgM(-) B cells in the IPP that supply early, non-diversified "natural" SIgA antibodies to the gut lumen and a primary IgA response in serum.
authors: Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA. Electronic address: john-butler@uiowa.edu.
authors: John E
authors: JE
authors: Butler
authors: Department of Veterinary Sciences, South Dakota State University, Brooking, SD, USA.
authors: Kristina
authors: K
authors: Santiago-Mateo
authors: Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
authors: Nancy
authors: N
authors: Wertz
authors: College of Animal Science and Technology, Northwest A & F University, Yangling, China.
authors: Xiuzhu
authors: X
authors: Sun
authors: Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czech Republic. Electronic address: marek.sinkora@worldonline.cz.
authors: Marek
authors: M
authors: Sinkora
authors: Department of Veterinary Sciences, South Dakota State University, Brooking, SD, USA.
authors: David L
authors: DL
authors: Francis
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000916:Antibody Diversity; D001402:B-Lymphocytes / Q000502:physiology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D000069196:Gastrointestinal Microbiome / Q000276:immunology; D005856:Germ-Free Life; D007082:Ileum / Q000276:immunology*; D007071:Immunoglobulin A, Secretory / Q000235:genetics*; D017578:Immunoglobulin Class Switching; D007075:Immunoglobulin M / Q000235:genetics; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D010581:Peyer's Patches / Q000276:immunology*; D019936:Probiotics / Q000008:administration & dosage; D013552:Swine / Q000276:immunology*
chemical_list: D007071:Immunoglobulin A, Secretory; D007075:Immunoglobulin M
keywords: Blind ileal loops; Germfree animals; Ileal Peyers patches; Immunoglobulin A