title: Peptide-modified PELCL electrospun membranes for regulation of vascular endothelial cells.
abstract: The efficiency of biomaterials used in small vascular repair depends greatly on their ability to interact with vascular endothelial cells (VECs). Rapid endothelialization of the vascular grafts is a promising way to prevent thrombosis and intimal hyperplasia. In this work, modification of electrospun membranes of poly(ethylene glycol)-b-poly(l-lactide-co-ε-caprolactone) (PELCL) by three different peptides for regulation of VECs were studied in order to obtain ideal bioactive biomaterials as small diameter vascular grafts. QK (a mimetic peptide to vascular endothelial growth factor), Arg-Glu-Asp-Val (REDV, a specific adhesive peptide to VECs) and Val-Ala-Pro-Gly (VAPG, a specific adhesive peptide to vascular smooth muscle cells) were investigated. Surface properties of the modified membranes and the response of VECs were verified. It was found that protein adsorption and platelet adhesion were effectively suppressed with the introduction of QK, REDV or VAPG peptides on the PELCL electrospun membranes. Both QK- and REDV-modified electrospun membranes could accelerate the proliferation of VECs in the first 9days, and the QK-modified electrospun membrane promoted cell proliferation more significantly than the REDV-modified one. The REDV-modified PELCL membrane was the most favorable for VECs adhesion than QK- and VAPG-modified membranes. It was suggested that QK- or REDV-modified PELCL electrospun membranes may have great potential applications in cardiovascular biomaterials for rapid endothelialization in situ.
authors: School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300072, China.
authors: Fang
authors: F
authors: Zhou
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China.
authors: Xiaoling
authors: X
authors: Jia
authors: School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300072, China.
authors: Yang
authors: Y
authors: Yang
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China.
authors: Qingmao
authors: Q
authors: Yang
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China.
authors: Chao
authors: C
authors: Gao
authors: School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300072, China.
authors: Yunhui
authors: Y
authors: Zhao
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China; National Research Center for Rehabilitation Technical Aids, Beijing 100176, China. Electronic address: yubofan@buaa.edu.cn.
authors: Yubo
authors: Y
authors: Fan
authors: School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300072, China. Electronic address: yuanxy@tju.edu.cn.
authors: Xiaoyan
authors: X
authors: Yuan
mesh_terms: D049109:Cell Proliferation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008567:Membranes, Artificial; D009842:Oligopeptides / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D009842:Oligopeptides; D011091:Polyesters; C000610191:arginyl-glutamyl-aspartyl-valine; C000613452:poly(ethylene glycol)-b-poly(lactide-co-epsilon-caprolactone); D011092:Polyethylene Glycols; C083364:valyl-alanyl-prolyl-glycine
keywords: Electrospun membrane; Poly(ethylene glycol)-b-poly(l-lactide-co-ε-caprolactone); QK peptide; REDV peptide; Vascular endothelial cells