title: A molecularly imprinted electrochemiluminescence sensor based on the mimetic enzyme catalytic effect for ultra-trace Ni2+determination.
abstract: A novel molecularly imprinted polymer (MIP) electrochemiluminescence (MIP-ECL) sensor was developed for the highly sensitive and selective determination of ultra-trace levels of Ni2+. The complex Ni2+-dimethylglyoxime (Ni-DMG) was chosen as the template molecule to construct the MIP and then acted as a mimetic enzyme to catalyse the oxidisation of luminol to enhance the ECL signal. When the imprinted cavities were occupied by Ni-DMG in the rebinding process, the ECL intensities produced by the luminol-H2O2ECL system on the MIP-modified electrode surface increased with increased concentration of the Ni-DMG complex. The highly sensitive determination of Ni2+was achieved through a catalytic reaction. This technique could be used for the quantitative analysis of Ni2+with concentrations from 3.0 × 10-12mol L-1to 6.0 × 10-9mol L-1. The detection limit was 1.01 × 10-12mol L-1, which is much lower than that reported previously. In addition, the allowable amounts of interference ions in the MIP-ECL sensor were higher than that in other common molecularly imprinted sensors because of its excellent recognition of 3D cavity-to-complex molecules and ligand-to-metal ions. This method was successfully used to determine Ni2+in real samples, such as apples, carrots and grapes, and has been proven feasible for practical applications.
authors: Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, PR China. likianping@263.com.
authors: Bin
authors: B
authors: Yang
authors: Jianping
authors: J
authors: Li
authors: Lianming
authors: L
authors: Zhang
authors: Guobao
authors: G
authors: Xu
mesh_terms: D002384:Catalysis; D004566:Electrodes; D005504:Food Analysis / Q000379:methods; D006861:Hydrogen Peroxide; D049449:Luminescence; D008165:Luminol / Q000737:chemistry*; D054802:Molecular Imprinting; D009532:Nickel / Q000032:analysis*; D011108:Polymers
chemical_list: D011108:Polymers; D008165:Luminol; D009532:Nickel; D006861:Hydrogen Peroxide