title: Association of interleukin-28B rs12979860 and rs8099917 polymorphisms with sustained viral response in hepatitis C virus genotype 1 and 3 infected patients from the Indian subcontinent.
abstract: BACKGROUND
  Polymorphisms of the IL28B gene (rs12979860 and rs8099917) have been shown to impact treatment responses in hepatitis C virus (HCV) infected patients. The association of these polymorphisms with sustained viral response (SVR) has been studied in HCV genotype 3 infected patients in India, but not in genotype 1.
  
  
  OBJECTIVE
  This study aimed to determine the association of IL28B gene polymorphisms and other host and viral factors with treatment response in patients with HCV genotype 1 and 3 infection.
  
  
  METHODS
  DNA from 42 HCV-infected patients on antiviral therapy was analysed for the IL28B polymorphisms using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Bidirectional sequencing was performed on a subset of samples for verification of PCR-RFLP results. Information on age, weight, height, diabetic status, pre-treatment viral load and alanine aminotransferase (ALT) levels was obtained from clinical records. The IL28B genotypes and the other factors were analysed for their association with SVR.
  
  
  RESULTS
  The frequency distribution of rs12979860 CC/CT/TT genotypes was found to be 66.7%, 26.2% and 7.1%, respectively. For rs8099917 genotype, the TT/GT/GG distribution was 73.8%, 21.4% and 4.8%, respectively. SVR was seen in 61.9% of cases (55.6% in genotype 1 and 62.5% in genotype 3). CC genotype at rs12979860 and TT genotype at rs8099917 were significantly higher in responders (P = 0.013 and 0.042, respectively). Lower baseline ALT and rapid viral response were also found to be associated with SVR. On logistic regression analysis, CC genotype at rs12979860 emerged as the most powerful predictor of treatment response.
  
  
  CONCLUSIONS
  IL28B polymorphisms are strong predictors of SVR in patients from the Indian subcontinent infected with HCV genotype 3 and genotype 1.
authors: Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India.
authors: P
authors: P
authors: Ranjan
authors: Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India.
authors: G J
authors: GJ
authors: Fletcher
authors: Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India.
authors: M
authors: M
authors: Radhakrishnan
authors: Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India.
authors: J
authors: J
authors: Sivakumar
authors: Department of Biostatistics, Christian Medical College, Vellore, Tamil Nadu, India.
authors: P S
authors: PS
authors: Premkumar
authors: Department of Hepatology, Christian Medical College, Vellore, Tamil Nadu, India.
authors: A
authors: A
authors: Goel
authors: Department of Hepatology, Christian Medical College, Vellore, Tamil Nadu, India.
authors: U G
authors: UG
authors: Zachariah
authors: Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India.
authors: P
authors: P
authors: Abraham
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000998:Antiviral Agents / Q000627:therapeutic use*; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019698:Hepatitis C, Chronic / Q000188:drug therapy* / Q000821:virology; D006801:Humans; D007194:India; D007372:Interferons; D007378:Interleukins / Q000235:genetics*; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D000072230:Sustained Virologic Response; D055815:Young Adult; D000095022:Interferon Lambda
chemical_list: D000998:Antiviral Agents; D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda