title: Review of bioanalytical assays for the quantitation of various HDAC inhibitors such as vorinostat, belinostat, panobinostat, romidepsin and chidamine.
abstract: Histone deacetylase inhibitors (HDAC inhibitors) are used to treat malignancies such as cutaneous T cell lymphoma and peripheral T cell lymphoma. Only four drugs are approved by the US Food and Drug Administration, namely vorinostat, romidepsin, panobinostat and belinostat, while chidamide has been approved in China. There are a number of bioanalytical methods reported for the measurement of HDAC inhibitors in clinical (human plasma and serum) and preclinical (mouse plasma, rat plasma, urine and tissue homogenates, etc.) studies. This review covers various HDAC inhibitors such as vorinostat, romidepsin, panobinostat, belinostat and chidamide. In addition to providing a comprehensive review of the available methods for the above mentioned HDAC inhibitors, it also provides case studies with perspectives for chosen drugs. Based on the review, it is concluded that the published methodologies using either HPLC or LC-MS/MS are well suited for the quantification of HDAC inhibitors in various biological fluids to delineate pharmacokinetic data.
authors: Jubilant Biosys, 2nd Stage, Bangalore, India.
authors: P S
authors: PS
authors: Suresh
authors: Jubilant Biosys, 2nd Stage, Bangalore, India.
authors: V C
authors: VC
authors: Devaraj
authors: Suramus Bio, Drug Development, Bangalore, India.
authors: Nuggehally R
authors: NR
authors: Srinivas
authors: Jubilant Biosys, 2nd Stage, Bangalore, India.
authors: Ramesh
authors: R
authors: Mullangi
mesh_terms: D000631:Aminopyridines / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D000818:Animals; D001549:Benzamides / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D047630:Depsipeptides / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D056572:Histone Deacetylase Inhibitors / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D006801:Humans; D006877:Hydroxamic Acids / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D007211:Indoles / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D009369:Neoplasms / Q000188:drug therapy; D000077767:Panobinostat; D013449:Sulfonamides / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000077337:Vorinostat
chemical_list: D000631:Aminopyridines; D001549:Benzamides; D047630:Depsipeptides; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D007211:Indoles; D013449:Sulfonamides; D000077337:Vorinostat; C547816:N-(2-amino-5-fluorobenzyl)-4-(N-(pyridine-3-acrylyl)aminomethyl)benzamide; D000077767:Panobinostat; C087123:romidepsin; C487081:belinostat
keywords: HPLC; LC-MS/MS; belinostat; bioanalytical methods; chidamine; panobinostat; review; romidepsin; vorinostat