title: A simultaneous assessment metric for MAb quantity and glycan quality.
abstract: As a critical quality attribute, glycosylation represents an important consideration when analyzing the success of a glycoprotein production process. Though critical, glycosylation is not the only measure of culture success; other factors, including culture size, maintenance, and productivity, are also critical. A new metric was developed to address both product quality, as measured through glycosylation, and product quantity, as measured through product concentration. A monoclonal antibody Chinese hamster ovary cell culture model system was used to assess this metric across various media formulations. In a model test system, the metric discriminated that some media supplements had a net positive impact on productivity and glycosylation, while others had a net negative impact on productivity and glycosylation.
authors: University of Guelph, Guelph, Canada. gerrydrouillard@gmail.com.
authors: Gerald
authors: http://orcid.org/0000-0002-9955-303X
authors: G
authors: Drouillard
authors: University of Guelph, Guelph, Canada.
authors: Gordon
authors: G
authors: Hayward
authors: University of Guelph, Guelph, Canada.
authors: Julie
authors: J
authors: Vale
authors: University of Guelph, Guelph, Canada.
authors: Roshni
authors: R
authors: Dutton
keywords: Chinese hamster ovary cells (CHO); Critical quality attributes; Glycosylation; Monoclonal antibodies; Productivity