title: The dietary effect of milk sphingomyelin on the lipid metabolism of obese/diabetic KK-A(y) mice and wild-type C57BL/6J mice.
abstract: Purified milk sphingomyelin (SM) was obtained from lipid concentrated butter serum (LC-BS) by successive separations involving solvent fractionation, selective saponification, and silicic acid column chromatography. The SM obtained was given to obese/diabetic KK-A(y) mice and wild-type C57BL/6J mice. SM supplementation significantly increased fecal lipids paralleled with a decrease in non-HDL cholesterol levels in the serum and neutral lipids and in cholesterol levels in the livers of KK-A(y) mice. The reduction of liver lipid levels also resulted in a decrease in the total fatty acid content of the KK-A(y) mice livers, while n-3 fatty acids derived from the conversion of α-linolenic acid (18:3n-3) increased due to SM supplementation. In contrast to the KK-A(y) mice, little change in the serum and liver lipids was observed in wild-type C57BL/6J mice. The present study suggests that SM may be effective only in subjects with metabolic disorders.
authors: Laboratory of Bio-functional Material Chemistry, Division of Marine Bioscience, Faculty of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan. kmiya@fish.hokudai.ac.jp.
authors: Ippei
authors: I
authors: Yamauchi
authors: Mariko
authors: M
authors: Uemura
authors: Masashi
authors: M
authors: Hosokawa
authors: Ai
authors: A
authors: Iwashima-Suzuki
authors: Makoto
authors: M
authors: Shiota
authors: Kazuo
authors: K
authors: Miyashita
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood; D003920:Diabetes Mellitus / Q000378:metabolism*; D004032:Diet; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000032:analysis / Q000097:blood; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D008892:Milk / Q000737:chemistry*; D009765:Obesity / Q000378:metabolism*; D013109:Sphingomyelins / Q000008:administration & dosage*; D017962:alpha-Linolenic Acid / Q000378:metabolism
chemical_list: D015525:Fatty Acids, Omega-3; D008055:Lipids; D013109:Sphingomyelins; D017962:alpha-Linolenic Acid; D002784:Cholesterol