title: Autophagy-associated alpha-arrestin signaling is required for conidiogenous cell development in Magnaporthe oryzae.
abstract: Conidiation patterning is evolutionarily complex and mechanism concerning conidiogenous cell differentiation remains largely unknown. Magnaporthe oryzae conidiates in a sympodial way and uses its conidia to infect host and disseminate blast disease. Arrestins are multifunctional proteins that modulate receptor down-regulation and scaffold components of intracellular trafficking routes. We here report an alpha-arrestin that regulates patterns of conidiation and contributes to pathogenicity in M. oryzae. We show that disruption of ARRDC1 generates mutants which produce conidia in an acropetal array and ARRDC1 significantly affects expression profile of CCA1, a virulence-related transcription factor required for conidiogenous cell differentiation. Although germ tubes normally develop appressoria, penetration peg formation is dramatically impaired and Δarrdc1 mutants are mostly nonpathogenic. Fluorescent analysis indicates that EGFP-ARRDC1 puncta are well colocalized with DsRed2-Atg8, and this distribution profile could not be altered in Δatg9 mutants, suggesting ARRDC1 enters into autophagic flux before autophagosome maturation. We propose that M. oryzae employs ARRDC1 to regulate specific receptors in response to conidiation-related signals for conidiogenous cell differentiation and utilize autophagosomes for desensitization of conidiogenous receptor, which transmits extracellular signal to the downstream elements of transcription factors. Our investigation extends novel significance of autophagy-associated alpha-arrestin signaling to fungal parasites.
authors: State Key Laboratory of Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China.
authors: Bo
authors: B
authors: Dong
authors: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321104, Zhejiang Province, China.
authors: Xiaojin
authors: X
authors: Xu
authors: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang Province, China.
authors: Guoqing
authors: G
authors: Chen
authors: State Key Laboratory of Rice Biology and Key Laboratory of Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China.
authors: Dandan
authors: D
authors: Zhang
authors: State Key Laboratory of Rice Biology and Key Laboratory of Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China.
authors: Mingzhi
authors: M
authors: Tang
authors: Institute of Digital Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China.
authors: Fei
authors: F
authors: Xu
authors: State Key Laboratory of Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou 310058, Zhejiang Province, China.
authors: Xiaohong
authors: X
authors: Liu
authors: Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China.
authors: Hua
authors: H
authors: Wang
authors: International Rice Research Institute, DAPO Box 7777, Metro Manila 1301, Philippines.
authors: Bo
authors: B
authors: Zhou
mesh_terms: D019393:Arrestin / Q000235:genetics / Q000378:metabolism*; D001343:Autophagy; D020869:Gene Expression Profiling; D055786:Gene Knockout Techniques; D020082:Magnaporthe / Q000166:cytology* / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D015398:Signal Transduction; D013172:Spores, Fungal / Q000166:cytology* / Q000254:growth & development*; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D019393:Arrestin; D014157:Transcription Factors; D037521:Virulence Factors