title: Bacterial Diversity and Community Structure of Supragingival Plaques in Adults with Dental Health or Caries Revealed by 16S Pyrosequencing.
abstract: Dental caries has a polymicrobial etiology within the complex oral microbial ecosystem. However, the overall diversity and structure of supragingival plaque microbiota in adult dental health and caries are not well understood. Here, 160 supragingival plaque samples from patients with dental health and different severities of dental caries were collected for bacterial genomic DNA extraction, pyrosequencing by amplification of the 16S rDNA V1-V3 hypervariable regions, and bioinformatic analysis. High-quality sequences (2,261,700) clustered into 10,365 operational taxonomic units (OTUs; 97% identity), representing 453 independent species belonging to 122 genera, 66 families, 34 orders, 21 classes, and 12 phyla. All groups shared 7522 OTUs, indicating the presence of a core plaque microbiome. α diversity analysis showed that the microbial diversity in healthy plaques exceeded that of dental caries, with the diversity decreasing gradually with the severity of caries. The dominant phyla of plaque microbiota included Bacteroidetes, Actinobacteria, Proteobacteria, Firmicutes, Fusobacteria, and TM7. The dominant genera included Capnocytophaga, Prevotella, Actinomyces, Corynebacterium, Neisseria, Streptococcus, Rothia, and Leptotrichia. β diversity analysis showed that the plaque microbial community structure was similar in all groups. Using LEfSe analysis, 25 differentially abundant taxa were identified as potential biomarkers. Key genera (27) that potentially contributed to the differential distributions of plaque microbiota between groups were identified by PLS-DA analysis. Finally, co-occurrence network analysis and function predictions were performed. Treatment strategies directed toward modulating microbial interactions and their functional output should be further developed.
authors: Shanghai Key Laboratory of Stomatology, Department of Endodontics and Operative Dentistry, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
authors: Cuicui
authors: C
authors: Xiao
authors: Shanghai Key Laboratory of Stomatology, Department of Endodontics and Operative Dentistry, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
authors: Shujun
authors: S
authors: Ran
authors: Shanghai Key Laboratory of Stomatology, Department of Endodontics and Operative Dentistry, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
authors: Zhengwei
authors: Z
authors: Huang
authors: Shanghai Key Laboratory of Stomatology, Department of Endodontics and Operative Dentistry, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
authors: Jingping
authors: J
authors: Liang
keywords: 16S rDNA; bacterial diversity; dental caries; dental plaque; pyrosequencing