title: Surveyor assay to diagnose persistent Müllerian duct syndrome in Miniature Schnauzers.
abstract: Persistent Müllerian duct syndrome (PMDS) is a pseudohermaphroditism in males characterized by the presence of Müllerian duct derivatives. As PMDS dogs often lack clinical symptoms, a molecular diagnosis is essential to identify the syndrome in these animals. In this study, a new molecular method using DNA mismatch-specific Surveyor nuclease was developed. The Surveyor nuclease assay identified theAMHR2mutation that produced PMDS in a Miniature Schnauzer as accurately as that obtained by using the conventional method based on restriction digestion. As an alternative to the current molecular diagnostic method, the new method may result in increased accuracy when detecting PMDS.
authors: Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Korea.
authors: Young June
authors: YJ
authors: Kim
authors: Dream Animal Hospital, Seoul 05570, Korea.
authors: Hyuk Jin
authors: HJ
authors: Kwon
authors: Dream Animal Hospital, Seoul 05570, Korea.
authors: Hyuk Soo
authors: HS
authors: Byun
authors: Dream Animal Hospital, Seoul 05570, Korea.
authors: Donguk
authors: D
authors: Yeom
authors: Dream Animal Hospital, Seoul 05570, Korea.
authors: Jea-Hong
authors: JH
authors: Choi
authors: Institute of Tissue Regeneration Engineering, Dankook University, Cheonan 31116, Korea.
authors: Joong-Hyun
authors: JH
authors: Kim
authors: Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Korea.|Institute of Tissue Regeneration Engineering, Dankook University, Cheonan 31116, Korea.|Department of Physiology, Dankook University School of Medicine, Cheonan 31116, Korea.
authors: Hosup
authors: H
authors: Shim
mesh_terms: D000818:Animals; D058490:Disorder of Sex Development, 46,XY / Q000175:diagnosis* / Q000235:genetics; D004283:Dog Diseases / Q000175:diagnosis* / Q000235:genetics; D004285:Dogs; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods / Q000662:veterinary*; D018000:Receptors, Peptide; D018125:Receptors, Transforming Growth Factor beta
chemical_list: D018000:Receptors, Peptide; D018125:Receptors, Transforming Growth Factor beta; C084621:anti-Mullerian hormone receptor
keywords: Miniature Schnauzer; persistent Müllerian duct syndrome; surveyor nuclease assay