title: LncRNA NONRATT021972 siRNA rescued decreased heart rate variability in diabetic rats in superior cervical ganglia.
abstract: Diabetic cardiac autonomic neuropathy (DCAN) is a serious and common complication in diabetes mellitus (DM). Long noncoding RNAs (lncRNAs), an important class of regulatory molecules in diverse biological processes, have attracted considerable interest in DCAN. Our previous study has indicated a lncRNA, NONRATT021972 (NONCODE ID), was enhanced in sympathetic neuronal-like PC12 cells in the setting of high glucose (HG) and high FFAs (HF); its silence was found to significantly alleviate HGHF-induced tumor necrosis factor-α (TNF-α) release in PC12 cells. Here we further explore the effects of NONRATT021972 small interference RNA (siRNA) on heart rate variability (HRV) mediated by superior cervical ganglia (SCG) in diabetic rats and the possible mechanism underlying. We found an increment of NONRATT021972 in SCG of DM rats. Treatment of NONRATT021972 siRNA in DM rats decreased the elevated expression of TNF-α, blocked serine phosphorylation of insulin receptor substrate (IRS) 1 and increased the down-regulated expression of IRS1 in SCG. Meanwhile, NONRATT021972 siRNA rescued decreased HRV in DM rats. Therefore, inhibition of NONRATT021972 may serve as a novel therapeutic strategy for preventing the development of DCAN.
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors: Hong
authors: H
authors: Xu
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors: Changle
authors: C
authors: Liu
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors: Shenqiang
authors: S
authors: Rao
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors: Luling
authors: L
authors: He
authors: Nanchang Institute of Science and Technology, Nanchang, Jiangxi 330006, PR China.
authors: Tengling
authors: T
authors: Zhang
authors: Second Clinical Medical College, Medical College of Nanchang University, Nanchang, 330008, P.R., China.
authors: Shanshan
authors: S
authors: Sun
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors: Bing
authors: B
authors: Wu
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors: Lifang
authors: L
authors: Zou
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors: Shouyu
authors: S
authors: Wang
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors: Yun
authors: Y
authors: Xue
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors: Tianyu
authors: T
authors: Jia
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors: Shanhong
authors: S
authors: Zhao
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors: Guilin
authors: G
authors: Li
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors: Shuangmei
authors: S
authors: Liu
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China; Department of Clinical Research, Singapore General Hospital, Singapore.
authors: Guodong
authors: G
authors: Li
authors: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China. Electronic address: liangsd@hotmail.com.
authors: Shangdong
authors: S
authors: Liang
mesh_terms: D000818:Animals; D001145:Arrhythmias, Cardiac / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D002574:Cervical Vertebrae; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D058065:Diabetic Cardiomyopathies / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000473:pathology; D006339:Heart Rate / Q000502:physiology; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism; D008297:Male; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000008:administration & dosage; D000067492:RNAi Therapeutics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha; C000611529:long non-coding RNA NONRATT021972, rat
keywords: Diabetic cardiac autonomic neuropathy; Insulin receptor substrate; Long noncoding RNA; Superior cervical ganglia