title: Dendritic Cells from HIV Controllers Have Low Susceptibility to HIV-1 Infection In Vitro but High Capacity to Capture HIV-1 Particles.
abstract: HIV controllers (HICs), rare HIV-1 infected individuals able to control viral replication without antiretroviral therapy, are characterized by an efficient polyfunctional and cytolytic HIV-specific CD8+ T cell response. The mechanisms underlying the induction and maintenance of such response in many HICs despite controlled viremia are not clear. Dendritic cells play a crucial role in the generation and reactivation of T cell responses but scarce information is available on those cells in HICs. We found that monocyte derived dendritic cells (MDDCs) from HICs are less permissive to HIV-1 infection than cells from healthy donors. In contrast MDDCs from HICs are particularly efficient at capturing HIV-1 particles when compared to cells from healthy donors or HIV-1 patients with suppressed viral load on antiretroviral treatment. MDDCs from HICs expressed on their surface high levels of syndecan-3, DC-SIGN and MMR, which could cooperate to facilitate HIV-1 capture. The combination of low susceptibility to HIV-1 infection but enhanced capacity to capture particles might allow MDDCs from HICs to preserve their function from the deleterious effect of infection while facilitating induction of HIV-specific CD8+ T cells by cross-presentation in a context of low viremia.
authors: Institut Pasteur, Régulation des Infections Rétrovirales, Paris, France.
authors: Chiraz
authors: C
authors: Hamimi
authors: Institut Pasteur, HIV Inflammation et Persistance, Paris, France.
authors: Annie
authors: A
authors: David
authors: Institut Pasteur, Régulation des Infections Rétrovirales, Paris, France.
authors: Pierre
authors: P
authors: Versmisse
authors: Institut Pasteur, Régulation des Infections Rétrovirales, Paris, France.|AP-HP Hôpital Européen Georges Pompidou, Paris, France.|Université Paris Descartes; Sorbonne Paris-Cité; Paris, France.
authors: Laurence
authors: L
authors: Weiss
authors: Université Paris Sud, UMR-1184, Le Kremlin-Bicêtre, France.|CEA, DSV/iMETI, Division of Immuno-Virology, IDMIT, Fontenay-aux-Roses, France.
authors: Timothée
authors: T
authors: Bruel
authors: Hopital Foch, Service de médecine interne, Suresnes, France.
authors: David
authors: D
authors: Zucman
authors: Sorbonne Universités, UPMC Univ Paris 06, DHU FAST, CR7, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Paris, France.|INSERM, U1135, CIMI-Paris, Paris, France.
authors: Victor
authors: V
authors: Appay
authors: Sorbonne Universités, UPMC Univ Paris 06, DHU FAST, CR7, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Paris, France.|INSERM, U1135, CIMI-Paris, Paris, France.|CNRS, ERL 8255, CIMI-Paris, Paris, France.
authors: Arnaud
authors: A
authors: Moris
authors: Institut Pasteur, Plate-forme Investigation Clinique et Accès aux Ressources Biologiques (ICAReB), Paris, France.
authors: Marie-Noëlle
authors: MN
authors: Ungeheuer
authors: AP-HP, Saint-Louis Hospital, Infectious Diseases Department, Paris, France.
authors: Caroline
authors: C
authors: Lascoux-Combe
authors: Institut Pasteur, Régulation des Infections Rétrovirales, Paris, France.
authors: Françoise
authors: F
authors: Barré-Sinoussi
authors: Institut Pasteur, HIV Inflammation et Persistance, Paris, France.
authors: Michaela
authors: M
authors: Muller-Trutwin
authors: INSERM U1018, Faculté de Médecine Paris Sud, Le Kremlin-Bicêtre, France.
authors: Faroudy
authors: F
authors: Boufassa
authors: Université Paris Sud, UMR-1184, Le Kremlin-Bicêtre, France.|CEA, DSV/iMETI, Division of Immuno-Virology, IDMIT, Fontenay-aux-Roses, France.|Inserm, U1184, Center for immunology of viral infections and autoimmune diseases, Le Kremlin-Bicêtre, France.|APHP, Hôpitaux Universitaires Paris Sud, Service de Médecine Interne-Immunologie Clinique, le Kremlin Bicêtre, France.
authors: Olivier
authors: O
authors: Lambotte
authors: Institut Pasteur, Régulation des Infections Rétrovirales, Paris, France.
authors: Gianfranco
authors: G
authors: Pancino
authors: Institut Pasteur, HIV Inflammation et Persistance, Paris, France.
authors: Asier
authors: A
authors: Sáez-Cirión
mesh_terms: D000956:Antigens, Viral / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D004198:Disease Susceptibility; D005786:Gene Expression Regulation; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism; D053670:Syndecan-3 / Q000378:metabolism; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D037181:Lectins, C-Type; D053670:Syndecan-3