title: Mms21 SUMO Ligase Activity Promotes Nucleolar Function in Saccharomyces cerevisiae.
abstract: The budding yeast E3 SUMO ligase Mms21, also known as Nse2, is a component of the Smc5/6 complex, which regulates sister chromatid cohesion, DNA replication, and repair. Our study shows that the mms21RINGΔ mutant exhibits (1) reduced ribosomal RNA production; (2) nuclear accumulation of ribosomal proteins; (3) elevated Gcn4 translation, indicating translational stress; and (4) upregulation of Gcn4 targets. Genes involved in ribosome biogenesis and translation are downregulated in the mms21RINGΔ mutant. We identified RPL19A as a novel genetic suppressor of the mms21RINGΔ mutant. Deletion of RPL19A partially suppresses growth defects in both smc5-6 and mms21RINGΔ mutants as well as nuclear accumulation of ribosome subunits in the mms21RINGΔ mutant. Deletion of a previously identified strong suppressor, MPH1, rescues both the accumulation of ribosome subunits and translational stress. This study suggests that the Smc5/6 complex supports nucleolar function.
authors: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors: Dong-Hwan
authors: DH
authors: Kim
authors: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors: Bethany
authors: B
authors: Harris
authors: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors: Fei
authors: F
authors: Wang
authors: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors: Chris
authors: C
authors: Seidel
authors: Stowers Institute for Medical Research, Kansas City, Missouri 64110.
authors: Scott
authors: S
authors: McCroskey
authors: Stowers Institute for Medical Research, Kansas City, Missouri 64110 Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160 jeg@stowers.org.
authors: Jennifer L
authors: JL
authors: Gerton
mesh_terms: D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics; D002466:Cell Nucleolus / Q000235:genetics / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D050505:Mutant Proteins / Q000235:genetics; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012269:Ribosomal Proteins / Q000235:genetics*; D012270:Ribosomes / Q000235:genetics; D025842:SUMO-1 Protein / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion / Q000235:genetics
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D018797:Cell Cycle Proteins; C501391:GCN4 protein, S cerevisiae; C500948:Mms21 protein, S cerevisiae; D050505:Mutant Proteins; D012335:RNA, Ribosomal; C102341:RPL19A protein, S cerevisiae; D012269:Ribosomal Proteins; C486627:SMC5 protein, S cerevisiae; C486631:SMC6 protein, S cerevisiae; D025842:SUMO-1 Protein; D029701:Saccharomyces cerevisiae Proteins; C491240:MPH1 protein, S cerevisiae; D053487:DEAD-box RNA Helicases
keywords: MMS21; MPH1; NSE2; RPL19A; Smc5/6; nucleolus; translational stress