title: Biological activity and binding properties of [Ru(II)(dcbpy)2Cl2] complex to bovine serum albumin, phospholipase A2 and glutathione.
abstract: Ruthenium compounds are highly regarded as metallo-drug candidates. Many studies have focused their attention on the interaction between ruthenium complexes with their possible biological targets. The interaction of ruthenium complexes with transport proteins, enzymes and peptides is of great importance for understanding their biodistribution and mechanism of action, therefore, the development of an anti-cancer therapy involving ruthenium complexes has recently shifted from DNA targeting towards protein targeting. With the aim of gaining insight into possible interactions between ruthenium complexes with biologically relevant proteins, we have studied the interaction of cis-dichlorobis(2,2'-bipyridyl-4,4'-dicarboxylic acid)ruthenium(II) complex [Ru(II)(dcbpy)2Cl2], which previously showed good potency in photo-dynamic chemotherapy, with bovine serum albumin (BSA), phospholipase A2 (PLA2) and glutathione (GSH). Binding constants and possible number of binding sites to mentioned proteins and peptide are investigated by ultraviolet-visible spectroscopy and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI TOF MS). The complex binding affinities were in the following order: PLA2 > BSA > GSH. Moreover, genotoxic profile of the complex, tested on peripheral blood lymphocytes as a model system, was also promising.
authors: Department of Physical Chemistry, Vinča Institute of Nuclear Sciences, University of Belgrade, Mike Petrovića Alasa 12-14, Belgrade, 11 000, Serbia. maki@vin.bg.ac.rs.
authors: Maja
authors: M
authors: Nešić
authors: Department of Physical Chemistry, Vinča Institute of Nuclear Sciences, University of Belgrade, Mike Petrovića Alasa 12-14, Belgrade, 11 000, Serbia.
authors: Iva
authors: I
authors: Popović
authors: Department of Physical Chemistry, Vinča Institute of Nuclear Sciences, University of Belgrade, Mike Petrovića Alasa 12-14, Belgrade, 11 000, Serbia.
authors: Andreja
authors: A
authors: Leskovac
authors: Department of Physical Chemistry, Vinča Institute of Nuclear Sciences, University of Belgrade, Mike Petrovića Alasa 12-14, Belgrade, 11 000, Serbia.
authors: Marijana
authors: M
authors: Petković
mesh_terms: D000328:Adult; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D005978:Glutathione / Q000737:chemistry*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000737:chemistry*; D010179:Pancreas / Q000201:enzymology; D054467:Phospholipases A2 / Q000737:chemistry* / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009942:Organometallic Compounds; D012710:Serum Albumin, Bovine; D012428:Ruthenium; D054467:Phospholipases A2; D005978:Glutathione
keywords: Biological targets; Glutathione; Potential metallo-drug; Proteins; Ruthenium (II) complex