title: Mucilaginibacter fluminis sp. nov., isolated from a freshwater river.
abstract: A bacterial strain, designated TTM-2T, was isolated from a water sample taken from the Caohu River in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain TTM-2T were Gram-stain-negative, aerobic, poly-β-hydroxybutyrate-accumulating, non-motile, rod-shaped and covered by large capsules; they formed light pink colonies. Growth occurred at 15-37 °C (optimum, 30 °C), at pH 4-8 (optimum, pH 6) and with 0-1 % (w/v) NaCl (optimum, 0.5 %, w/v). Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strain TTM-2T belonged to the genus Mucilaginibacter. The sequence similarities between the 16S rRNA genes of strain TTM-2T and the type strains of other species of the genus Mucilaginibacter ranged from 93.6 to 97.4 %. The closest relatives of strain TTM-2T were Mucilaginibactersoli R9-65T (97.4 %) and Mucilaginibactedefluvii A5T (97.1 %). The predominant fatty acids were iso-C15 : 0 (36.9 %) and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 33.7 %). The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major isoprenoid quinone was MK-7. The DNA G+C content of the genomic DNA was 46.6 mol%. The DNA-DNA relatedness of strain TTM-2T with respect to species of the genus Mucilaginibacterwith validly published names was less than 70 %. On the basis of the phylogenetic inference and phenotypic data, strain TTM-2T should be classified as a novel species, for which the name Mucilaginibacter fluminis sp. nov. is proposed. The type strain is TTM-2T (=BCRC 80785T=LMG 28455T=KCTC 42274T).
authors: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors: Shih-Yi
authors: SY
authors: Sheu
authors: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors: Yi-Ling
authors: YL
authors: Chen
authors: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors: Wen-Ming
authors: WM
authors: Chen
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006885:Hydroxybutyrates; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D011091:Polyesters; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013624:Taiwan; D000094122:Polyhydroxybutyrates
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006885:Hydroxybutyrates; D010743:Phospholipids; D011091:Polyesters; D012336:RNA, Ribosomal, 16S; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates