title: Synthesis of saturated, unsaturated, spin-labeled, and fluorescent cholesteryl esters: Acylation of cholesterol using fatty acid anhydride and 4-pyrrolidinopyridine.
abstract: A rapid, high yield method for the preparation of cholesteryl esters is described. The method is a modification of the catalytic procedure previously applied to the acylation of sn-glycero-3-phosphoryl-choline (Patel, K.M., J.D. Morrisett, and J.T. Sparrow, J. Lipid Res., 20:676 (1979). Cholesteryl esters are formed in excellent yield by acylating cholesterol with fatty acid anhydride or fatty acid and dicyclohexylcarbodiimide in methylene chloride containing 4-pyrrolidinopyridine. The versatility of the method is demonstrated by the preparation of the cholesteryl esters of saturated, unsaturated, spinlabeled, and labile fluorescent fatty acids.
authors: The Department of Medicine, Baylor College of Medicine and The Methodist Hospital, 77030, Houston, Texas.
authors: K M
authors: KM
authors: Patel
authors: The Department of Medicine, Baylor College of Medicine and The Methodist Hospital, 77030, Houston, Texas.
authors: L A
authors: LA
authors: Sklar
authors: The Department of Medicine, Baylor College of Medicine and The Methodist Hospital, 77030, Houston, Texas.
authors: R
authors: R
authors: Currie
authors: The Department of Medicine, Baylor College of Medicine and The Methodist Hospital, 77030, Houston, Texas.
authors: H J
authors: HJ
authors: Pownall
authors: The Department of Medicine, Baylor College of Medicine and The Methodist Hospital, 77030, Houston, Texas.
authors: J D
authors: JD
authors: Morrisett
authors: The Department of Medicine, Baylor College of Medicine and The Methodist Hospital, 77030, Houston, Texas.
authors: J T
authors: JT
authors: Sparrow