title: The Chromosome Microdissection and Microcloning Technique.
abstract: Chromosome microdissection followed by microcloning is an efficient tool combining cytogenetics and molecular genetics that can be used for the construction of the high density molecular marker linkage map and fine physical map, the generation of probes for chromosome painting, and the localization and cloning of important genes. Here, we describe a modified technique to microdissect a single chromosome, paint individual chromosomes, and construct single-chromosome DNA libraries.
authors: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, 100101, China.|Graduate University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors: Ying-Xin
authors: YX
authors: Zhang
authors: Henan Normal University, Xinxiang, 453007, China.
authors: Chuan-Liang
authors: CL
authors: Deng
authors: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, 100101, China. zmhu@genetics.ac.cn.
authors: Zan-Min
authors: ZM
authors: Hu
mesh_terms: D020223:Chromosome Painting / Q000379:methods; D032461:Chromosomes, Plant; D003001:Cloning, Molecular / Q000379:methods*; D015723:Gene Library; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D042282:Microdissection / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012434:Secale / Q000235:genetics*
keywords: Chromosome microdissection and microcloning; Degenerated oligonucleotide-primed-PCR; Fluorescence in situ hybridization; Single-chromosome library