title: Involvement of Protein Kinase C-δ in the Realization of Cardioprotective Effect of Ischemic Postconditioning.
abstract: Experiments on isolated perfused rat heart modeled 45-min global ischemia followed by 30-min reperfusion. Ischemic postconditioning was modeled by 3 cycles of reperfusion (30 sec) and ischemia (30 sec). Cardiomyocyte necrosis was assessed by the level of creatine phosphokinase in the perfusate. Postconditioning reduced the release of creatine phosphokinase from the heart by 30%. The cardioprotective effect of ischemic postconditioning was eliminated after inhibition of protein kinase C with cheleritrin or after blockade of δ-isoform of protein kinase C with rottlerin. These findings attest to participation of protein kinase C-δ in the realization of the cardioprotective effect of postconditioning.
authors: Research Institute of Cardiology, Tomsk, Russia.
authors: Yu B
authors: YB
authors: Lishmanov
authors: Research Institute of Cardiology, Tomsk, Russia.
authors: A S
authors: AS
authors: Gorbunov
authors: Research Institute of Cardiology, Tomsk, Russia. maslov@cardio-tomsk.ru.
authors: L N
authors: LN
authors: Maslov
mesh_terms: D000098:Acetophenones / Q000494:pharmacology; D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology; D003402:Creatine Kinase / Q000378:metabolism; D057775:Ischemic Postconditioning; D019157:Ischemic Preconditioning, Myocardial; D008297:Male; D032383:Myocytes, Cardiac / Q000201:enzymology*; D051745:Protein Kinase C-delta / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000098:Acetophenones; D001578:Benzopyrans; C085746:rottlerin; D051745:Protein Kinase C-delta; D003402:Creatine Kinase
keywords: heart; ischemic postconditioning; protein kinase C