title: Effect of Urinary Kallidinogenase on Transforming Growth Factor-β1 and High-Sensitivity C-Reactive Protein Expression in Rat Focal Cerebral Ischemic Injury.
abstract: BACKGROUND In this study we investigated the effect of urinary kallidinogenase (UK) on transforming growth factor beta 1 (TGF-β1) expression in brain tissue. We also explored the neuroprotective mechanism of UK against ischemic injury by measuring serum high-sensitivity C-reactive protein (hs-CRP) level changes after rat cerebral ischemic injury. MATERIAL AND METHODS The rat middle cerebral artery ischemia/reperfusion model was established using the suture method. Sprague-Dawley rats were randomly divided into 3 groups: treatment, Gegen control, and blank control. Each group was subsequently divided into 5 subgroups according to time (6, 12, 24, 48, and 72 h). Rats in the treatment group were administered UK as treatment. TGF-β1 expression was observed at each time point using SABC and immunohistochemical staining methods to estimate cerebral infarct volume percentage. Serum hs-CRP levels were also measured. RESULTS TGF-β1 protein expression in ischemic brain tissues of the treatment group significantly increased at each time point (P<0.01) compared with both control groups. Treatment group serum hs-CRP levels significantly decreased at each time point (P<0.05) compared with both control groups. CONCLUSIONS UK exerts a neuroprotective effect by upregulating TGF-β1 expression and inhibiting excessive inflammatory responses.
authors: Department of Pharmacy, The First Afiliated Hospital of Xinxiang Medical College, Weihui, Henan, China (mainland).
authors: Ting-Fang
authors: TF
authors: Dong
authors: School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan, China (mainland).
authors: Hai-Xia
authors: HX
authors: Lv
authors: Department of Neurology, The First Afiliated Hospital of Xinxiang Medical College, Weihui, Henan, China (mainland).
authors: Xiao-Lu
authors: XL
authors: Niu
authors: Department of Neurology, The First Afiliated Hospital of Xinxiang Medical College, Weihui, Henan, China (mainland).
authors: Yong-Kun
authors: YK
authors: Gui
authors: Department of Neurology, The First Afiliated Hospital of Xinxiang Medical College, Weihui, Henan, China (mainland).
authors: Ping
authors: P
authors: Zhang
authors: Department of Neurology, The First Afiliated Hospital of Xinxiang Medical College, Weihui, Henan, China (mainland).
authors: Hai-Qing
authors: HQ
authors: Yan
authors: Department of Neurology, The First Afiliated Hospital of Xinxiang Medical College, Weihui, Henan, China (mainland).
authors: Tong
authors: T
authors: Li
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000201:enzymology / Q000378:metabolism* / Q000652:urine; D002097:C-Reactive Protein / Q000096:biosynthesis*; D004195:Disease Models, Animal; D007610:Kallikreins / Q000378:metabolism* / Q000652:urine; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000201:enzymology / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*
chemical_list: D053773:Transforming Growth Factor beta1; D002097:C-Reactive Protein; D007610:Kallikreins