title: Construction and characterization of osteogenic and vascular endothelial cell sheets from rat adipose-derived mesenchymal stem cells.
abstract: In this study, adipose-derived mesenchymal stem cells (ADSCs) were isolated from adipose tissues of rats. Flow cytometry identification showed that ADSCs of passage 3 highly expressed CD29 and CD44, but hardly expressed CD31 and CD45. Adipogenic, osteogenic, and chondrogenic differentiation were confirmed by the results of oil red O staining, alkaline phosphatase (ALP), and alcian blue staining, respectively. ADSCs at a density of 1×10(6)/cm(2) were cultured in the osteogenic medium and the osteogenic cell sheets could be obtained after 14 d. The cell sheets were positive with von kossa staining. The transmission electron microscopy (TEM) result showed that needle-like calcium salt crystals were deposited on the ECM. These results suggested that the osteogenic cell sheets may have potential osteogenesis ability. ADSCs at a density of 1×10(6)/cm(2) were cultured in the endothelial cell growth medium-2 and the endothelial cell sheets can be formed after 16 d of culture. The TEM image confirmed that the Weibel-Palade corpuscle was seen in the cells. The expression of CD31 was positive, suggesting that the endothelial cell sheets may have a strong ability to form blood vessels. In this study, two types of cell sheets with the potential abilities of osteogenesis and blood vessels formation were obtained by induced culture of ADSCs in vitro, which lays a foundation to build vascularized tissue engineered bone for the therapy of bone defects.
authors: College of Stomatology, Ningxia Medical University, Yinchuan 750004, China. Electronic address: hua31415926@163.com.
authors: Hualin
authors: H
authors: Zhang
authors: College of Stomatology, Ningxia Medical University, Yinchuan 750004, China.
authors: Na
authors: N
authors: Yu
authors: College of Stomatology, Ningxia Medical University, Yinchuan 750004, China.
authors: Yueli
authors: Y
authors: Zhou
authors: College of Stomatology, Ningxia Medical University, Yinchuan 750004, China.
authors: Hairong
authors: H
authors: Ma
authors: College of Stomatology, Ningxia Medical University, Yinchuan 750004, China.
authors: Juan
authors: J
authors: Wang
authors: College of Stomatology, Ningxia Medical University, Yinchuan 750004, China.
authors: Xuerong
authors: X
authors: Ma
authors: School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou 325027, China. Electronic address: cecily5178@163.com.
authors: Jinsong
authors: J
authors: Liu
authors: College of Stomatology, Ningxia Medical University, Yinchuan 750004, China.
authors: Jin
authors: J
authors: Huang
authors: College of Stomatology, Ningxia Medical University, Yinchuan 750004, China.
authors: Yilin
authors: Y
authors: An
mesh_terms: D017667:Adipocytes / Q000166:cytology; D050156:Adipogenesis / Q000235:genetics; D000273:Adipose Tissue / Q000166:cytology / Q000254:growth & development; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000235:genetics; D042783:Endothelial Cells / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis / Q000235:genetics; D051381:Rats; D023822:Tissue Engineering
keywords: ADSCs; Endothelial cell sheet; Endothelial differentiation; Osteogenic cell sheet; Osteogenic differentiation; Vascularized tissue engineered bone