title: Ischemic post-conditioning attenuates acute lung injury induced by intestinal ischemia-reperfusion in mice: role of Nrf2.
abstract: Intestinal ischemic post-conditioning (IPo) protects against lung injury induced by intestinal ischemia-reperfusion (IIR) partly through promotion of expression and function of heme oxygenase-1 (HO-1). NF-E2-related factor-2 (Nrf2) is a key transcription factor that interacts with HO-1 and regulates antioxidant defense. However, the role of Nrf2 in IPo protection of IIR-induced pulmonary injury is not completely understood. Here we show that IPo significantly attenuated IIR-induced lung injury and suppressed oxidative stress and systemic inflammatory responses. IPo also increased the expression of both Nrf2 and HO-1. Consistently, the beneficial effects of IPo were abolished by ATRA and Brusatol, potent inhibitors of Nrf2. Moreover, the Nrf2 agonist t-BHQ showed similar activity as IPo. Taken together, our data suggest that Nrf2 activity, along with HO-1, plays an important role in the protective effects of IPo against IIR-induced acute lung injury.
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Qing-Tao
authors: QT
authors: Meng
authors: The Medical Department, the 3rd Hospital of Wuhan, Wuhan, China.
authors: Chen
authors: C
authors: Cao
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Yang
authors: Y
authors: Wu
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Hui-Min
authors: HM
authors: Liu
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Wei
authors: W
authors: Li
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Qian
authors: Q
authors: Sun
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Rong
authors: R
authors: Chen
authors: Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Yong-Guang
authors: YG
authors: Xiao
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Ling-Hua
authors: LH
authors: Tang
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Ying
authors: Y
authors: Jiang
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Yan
authors: Y
authors: Leng
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Shao-Qing
authors: SQ
authors: Lei
authors: Department of Anesthesiology, Washington University School of Medicine, St Louis, MO, USA.
authors: Chris C
authors: CC
authors: Lee
authors: Department of Anesthesiology, Washington University School of Medicine, St Louis, MO, USA.
authors: Devin M
authors: DM
authors: Barry
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.|Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Xiangdong
authors: X
authors: Chen
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Zhong-Yuan
authors: ZY
authors: Xia
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D005979:Glutathione Peroxidase / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood; D007422:Intestines / Q000098:blood supply*; D057775:Ischemic Postconditioning; D008168:Lung / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008810:Mice, Inbred C57BL; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D011897:Random Allocation; D015427:Reperfusion Injury / Q000150:complications*; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015850:Interleukin-6; D008565:Membrane Proteins; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D013482:Superoxide Dismutase