title: Phytate-degrading enzyme production by bacteria isolated from Malaysian soil.
abstract: Over two hundred bacteria were isolated from the halosphere, rhizosphere and endophyte of Malaysian maize plantation and screened for phytases activity. Thirty isolates with high detectable phytase activity were chosen for media optimization study and species identification. Ten types of bacterial phytase producers have been discovered in this study, which provides opportunity for characterization of new phytase(s) and various commercial and environmental applications. The majority of the bacterial isolates with high detectable phytase activity were of endophyte origin and 1.6% of the total isolates showed phytase activity of more than 1 U/ml. Most of the strains produced extra-cellular phytase and Staphylococcus lentus ASUIA 279 showed the highest phytase activity of 1.913 U/ml. All 30 species used in media optimization study exhibit favorable enzyme production when 1% rice bran was included in the growth media.
authors: Biomolecular Engineering Research Unit, Department of Biotechnology Engineering, International Islamic University Malaysia, Jalan Gombak, 53100, Kuala Lumpur, Malaysia.
authors: Anis Shobirin Meor
authors: AS
authors: Hussin
authors: Biomolecular Engineering Research Unit, Department of Biotechnology Engineering, International Islamic University Malaysia, Jalan Gombak, 53100, Kuala Lumpur, Malaysia. farouk@iiu.edu.my.
authors: Abd-ElAziem
authors: AE
authors: Farouk
authors: Centre for Molecular Biology, Federal Research Centre for Nutrition and Food, Haid-und-Neu Strasse 9, 76131, Karlsruhe, Germany.
authors: Ralf
authors: R
authors: Greiner
authors: Biomolecular Engineering Research Unit, Department of Biotechnology Engineering, International Islamic University Malaysia, Jalan Gombak, 53100, Kuala Lumpur, Malaysia.
authors: Hamzah Mohd
authors: HM
authors: Salleh
authors: Biomolecular Engineering Research Unit, Department of Biotechnology Engineering, International Islamic University Malaysia, Jalan Gombak, 53100, Kuala Lumpur, Malaysia.
authors: Ahmad Faris
authors: AF
authors: Ismail
keywords: Media optimization; Screening; Soil bacteria; Zea mays; phytase