title: A red emitting two-photon fluorescent probe for dynamic imaging of redox balance meditated by a superoxide anion and GSH in living cells and tissues.
abstract: Cellular self-regulation of reactive oxygen species (ROS) stress via antioxidant repair plays an important role in maintaining the redox balance. The redox balance between reducing and oxidizing species within cells is significant in the regulation of a signal pathway and is achieved by a series of elaborate mechanisms. In this work, we employed our previously reported D-π-A-structured naphthalene-BODIPY TBET platform to design an efficient two-photon fluorescent probe for dynamic monitoring of superoxide anion oxidative stress and the GSH reducing repair process. The probe displayed high energy transfer efficiency (91.4%), large pseudo-Stokes shifts upon one-photon excitation, and red fluorescence emission (λem= 596 nm), which is highly desirable for bioimaging applications. The probe exhibits reversibility, rapid response, good photostability, high selectivity and sensitivity for the superoxide anion and GSH. More importantly, the probe was successfully applied for visualizing the redox changes in living cells and tissues.
authors: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China. xbzhang@hnu.edu.cn.
authors: Hong-Wen
authors: HW
authors: Liu
authors: Xiaoyan
authors: X
authors: Zhu
authors: Jing
authors: J
authors: Zhang
authors: Xiao-Bing
authors: XB
authors: Zhang
authors: Weihong
authors: W
authors: Tan
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes; D005978:Glutathione / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D008099:Liver / Q000000981:diagnostic imaging; D051379:Mice; D008819:Mice, Nude; D010084:Oxidation-Reduction; D017785:Photons; D017382:Reactive Oxygen Species / Q000032:analysis*; D013481:Superoxides / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D013481:Superoxides; D005978:Glutathione