title: Visualizing Flagella while Tracking Bacteria.
abstract: A complete description of the swimming behavior of a bacterium requires measurement of the displacement and orientation of the cell body together with a description of the movement of the flagella. We rebuilt a tracking microscope so that we could visualize flagellar filaments of tracked cells by fluorescence. We studied Escherichia coli (cells of various lengths, including swarm cells), Bacillus subtilis (wild-type and a mutant with fewer flagella), and a motile Streptococcus (now Enterococcus). The run-and-tumble statistics were nearly the same regardless of cell shape, length, and flagellation; however, swarm cells rarely tumbled, and cells of Enterococcus tended to swim in loops when moving slowly. There were events in which filaments underwent polymorphic transformations but remained in bundles, leading to small deflections in direction of travel. Tumble speeds were ∼2/3 as large as run speeds, and the rates of change of swimming direction while running or tumbling were smaller when cells swam more rapidly. If a smaller fraction of filaments were involved in tumbles, the tumble intervals were shorter and the angles between runs were smaller.
authors: Rowland Institute at Harvard, Harvard University, Cambridge, Massachusetts.
authors: Linda
authors: L
authors: Turner
authors: Ecmictics Biotechnology, Markham, Ontario, Canada.
authors: Liam
authors: L
authors: Ping
authors: Rowland Institute at Harvard, Harvard University, Cambridge, Massachusetts.
authors: Marianna
authors: M
authors: Neubauer
authors: Rowland Institute at Harvard, Harvard University, Cambridge, Massachusetts. Electronic address: hberg@mcb.harvard.edu.
authors: Howard C
authors: HC
authors: Berg
mesh_terms: D001419:Bacteria / Q000166:cytology*; D058948:Cell Tracking; D005407:Flagella / Q000378:metabolism*