title: Simultaneous analysis of heparosan oligosaccharides by isocratic liquid chromatography with charged aerosol detection/mass spectrometry.
abstract: Uncovering the biological roles of heparosan oligosaccharides requires a simple and robust method for their separation and identification. We reported on systematic investigations of the retention behaviors of synthetic heparosan oligosaccharides on porous graphitic carbon (PGC) column by HPLC with charged aerosol detection. Oligosaccharides were strongly retained by PGC material in water-acetonitrile mobile phase, and eluted by trifluoroacetic acid occurring as narrow peaks. Addition of small fraction of methanol led to better selectivity of PGC to oligosaccharides than acetonitrile modifier alone, presumably, resulting from displacement of methanol to give different chemical environment at the PGC surface. Van't-Hoff plots demonstrated that retention behaviors highly depended on the column temperature and oligosaccharide moieties. By implementing the optimal MeOH content and temperature, a novel isocratic elution method was successfully developed for baseline resolution and identification of seven heparosan oligosaccharides using PGC-HPLC-CAD/MS. This approach allows for rapid analysis of heparosan oligosaccharides from various sources.
authors: Key Laboratory of Chemical Biology (Ministry of Education), Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China.
authors: Xiaohu
authors: X
authors: Ji
authors: Key Laboratory of Chemical Biology (Ministry of Education), Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China.
authors: Guixin
authors: G
authors: Hu
authors: Key Laboratory of Chemical Biology (Ministry of Education), Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China.
authors: Qiongyan
authors: Q
authors: Zhang
authors: Key Laboratory of Chemical Biology (Ministry of Education), Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China; National Glycoengineering Research Center, Shandong University, Jinan 250012, Shandong, PR China.
authors: Fengshan
authors: F
authors: Wang
authors: Key Laboratory of Chemical Biology (Ministry of Education), Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China; National Glycoengineering Research Center, Shandong University, Jinan 250012, Shandong, PR China. Electronic address: liuchunhui@sdu.edu.cn.
authors: Chunhui
authors: C
authors: Liu
mesh_terms: D000336:Aerosols / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D004187:Disaccharides / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D009844:Oligosaccharides / Q000032:analysis*
chemical_list: D000336:Aerosols; D004187:Disaccharides; D009844:Oligosaccharides; C560363:heparosan
keywords: Charged aerosol detection; HPLC; Heparosan oligosaccharides; Isocratic elution; MS; Porous graphitic carbon