title: MiR-122 targets VEGFC in bladder cancer to inhibit tumor growth and angiogenesis.
abstract: Previous studies indicate that microRNA-122 (miR-122) is down-regulated in several cancer cells and regulates cell apoptosis, proliferation, metastasis, and tumor angiogenesis. However, the mount of miR-122 in bladder cancer and the pivotal molecular mechanisms of miR-122 used to regulate bladder carcinogenesis and angiogenesis remain to be clarified. Here, we reveal that miR-122 expression is down-regulated in human bladder cancer tissues and cell lines. MiR-122 represses vascular endothelial growth factor C (VEGFC) post-transcriptional expression by directly binding to its 3'-UTR. The protein kinase B (AKT) and mammalian target of rapamycin (mTOR), which are the most important downstream molecules of VEGFC, are also decreased in bladder cancer cell after miR-122 overexpression. Furthermore, miR-122 over-expression decreases bladder cancer cell migration, invasion, colony formation in vitro and slow bladder cancer growth and angiogenesis in vivo. Finally, miR-122 sensitizes bladder cancer cells to cisplatin-induced apoptosis. Taken together, these studies suggest that miR-122 serves as a tumor suppressor and down-regulating VEGFC expression, leading to the inhibition of bladder cancer growth and angiogenesis.
authors: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology No. 23, Pingjiang Road, Hexi District, Tianjin 300211, China.
authors: Yi
authors: Y
authors: Wang
authors: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology No. 23, Pingjiang Road, Hexi District, Tianjin 300211, China.
authors: Qing-Fei
authors: QF
authors: Xing
authors: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology No. 23, Pingjiang Road, Hexi District, Tianjin 300211, China.
authors: Xiao-Qiang
authors: XQ
authors: Liu
authors: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology No. 23, Pingjiang Road, Hexi District, Tianjin 300211, China.
authors: Zhan-Jun
authors: ZJ
authors: Guo
authors: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology No. 23, Pingjiang Road, Hexi District, Tianjin 300211, China.
authors: Chang-Ying
authors: CY
authors: Li
authors: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology No. 23, Pingjiang Road, Hexi District, Tianjin 300211, China.
authors: Guang
authors: G
authors: Sun
keywords: MiR-122; VEGFC; angiogenesis; bladder cancer