title: Live Staining of Drosophila Embryos with RPTP Fusion Proteins to Detect and Characterize Expression of Cell-Surface RPTP Ligands.
abstract: The activity and/or localization of receptor tyrosine kinases and phosphatases are controlled by binding to cell-surface or secreted ligands. Identification of ligands for receptor tyrosine phosphatases (RPTPs) is essential for understanding their in vivo functions during development and disease. Here we describe a novel in vivo method to identify ligands and binding partners for RPTPs by staining live-dissected Drosophila embryos. Live dissected embryos are incubated with RPTP fusion proteins to detect ligand binding in embryos. This method can be streamlined to perform large-scale screens for ligands as well as to search for embryonic phenotypes.
authors: Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA, 91125, USA.
authors: Namrata
authors: N
authors: Bali
authors: Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA, 91125, USA.
authors: Hyung-Kook Peter
authors: HK
authors: Lee
authors: Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA, 91125, USA. zinnk@caltech.edu.
authors: Kai
authors: K
authors: Zinn
mesh_terms: D000818:Animals; D002462:Cell Membrane; D004210:Dissection / Q000379:methods; D004330:Drosophila / Q000196:embryology* / Q000378:metabolism; D029721:Drosophila Proteins / Q000032:analysis / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry / Q000379:methods*; D008024:Ligands; D008297:Male; D011485:Protein Binding; D054557:Receptor-Like Protein Tyrosine Phosphatases / Q000032:analysis / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D008024:Ligands; D011993:Recombinant Fusion Proteins; D054557:Receptor-Like Protein Tyrosine Phosphatases
keywords: Fusion proteins; Ligand screening; Live dissection; Receptor tyrosine phosphatases; Schneider cells