title: Dissecting Plant Chromosomes by the Use of Ionizing Radiation.
abstract: Radiation treatment of genomes is used to generate chromosome breaks for numerous applications. This protocol describes the preparation of seeds and the determination of the optimal level of irradiation dosage for the creation of a radiation hybrid (RH) population. These RH lines can be used to generate high-resolution physical maps for the assembly of sequenced genomes as well as the fine mapping of genes. This procedure can also be used for mutation breeding and forward/reverse genetics.
authors: Department of Horticultural Science, University of Minnesota, 1970 Folwell Ave., St. Paul, MN, 55108, USA. kiani002@umn.edu.
authors: Penny M A
authors: PM
authors: Kianian
authors: USDA-ARS, Cereal Disease Laboratory, Department of Plant Pathology, University of Minnesota, 1551 Lindig Ave., St. Paul, MN, 55108, USA.
authors: Katie L
authors: KL
authors: Liberatore
authors: Department of Horticultural Science, University of Minnesota, 1970 Folwell Ave., St. Paul, MN, 55108, USA.
authors: Marisa E
authors: ME
authors: Miller
authors: Department of Plant Sciences, North Dakota State University, Fargo, ND, USA.
authors: Justin B
authors: JB
authors: Hegstad
authors: USDA-ARS, Cereal Disease Laboratory, Department of Plant Pathology, University of Minnesota, 1551 Lindig Ave., St. Paul, MN, 55108, USA. shahryar.kianian@ars.usda.gov.
authors: Shahryar F
authors: SF
authors: Kianian
mesh_terms: D032461:Chromosomes, Plant / Q000528:radiation effects*; D018745:Genome, Plant; D021101:Radiation Hybrid Mapping / Q000379:methods*; D011839:Radiation, Ionizing; D014908:Triticum / Q000235:genetics* / Q000528:radiation effects
keywords: Gamma rays; Gene cloning; Mapping; Mutation; Radiation hybrid; Tempering seed