title: An Amperometric Immunosensor Based on an Ionic Liquid and Single-Walled Carbon Nanotube Composite Electrode for Detection of Tetrodotoxin in Pufferfish.
abstract: An amperometric immunosensor based on a composite electrode of single-walled carbon nanotubes and ionic liquid n-octylpyridinum afluorophosphate (SWCNT-ILE) was developed for the determination of tetrodotoxin (TTX). Compared with the glassy carbon electrode (GCE), the electrode combined advantages of carbon nanotubes and ionic liquid, which exhibited the excellent antifouling ability of p-nitrophenol (PNP) so that it remarkably improved the stability of the p-nitrophenyl phosphate-based sensor. Combining the enzyme-linked immune sorbent assay (ELISA) by alkaline phosphatase (AP) and magnetic particles immobilized with antigens, a real-time assay of tetrodotoxin was developed by amperometric immunosensors. Under the optimium condition, the developed sensor demonstrated a linear range of tetrodotoxin from 2 to 45 ng/mL with a low detection limit of 5 ng/mL. Furthermore, the amperometric immunosensor was applied to determine TTX in real samples and could be used as an effective and sensitive sensor for direct detection of tetrodotoxin within 20 min.
authors: College of Food Science & Technology, Shanghai Ocean University , Shanghai 201306, People's Republic of China.
authors: Yun
authors: Y
authors: Zhang
authors: College of Food Science & Technology, Shanghai Ocean University , Shanghai 201306, People's Republic of China.
authors: Yuxia
authors: Y
authors: Fan
authors: College of Biosystems Engineering and Food Science, Zhejiang University , Hangzhou 310058, People's Republic of China.
authors: Jian
authors: J
authors: Wu
authors: College of Food Science & Technology, Shanghai Ocean University , Shanghai 201306, People's Republic of China.
authors: Xichang
authors: X
authors: Wang
authors: College of Food Science & Technology, Shanghai Ocean University , Shanghai 201306, People's Republic of China.
authors: Yuan
authors: Y
authors: Liu
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D004566:Electrodes; D005504:Food Analysis / Q000379:methods*; D052578:Ionic Liquids / Q000737:chemistry*; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009596:Nitrophenols / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D011726:Pyridinium Compounds / Q000737:chemistry; D023721:Tetraodontiformes; D013779:Tetrodotoxin / Q000032:analysis*
chemical_list: D052578:Ionic Liquids; D037742:Nanotubes, Carbon; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D011726:Pyridinium Compounds; C008644:nitrophenylphosphate; D013779:Tetrodotoxin
keywords: alkaline phosphatase (AP); ionic liquid n-octylpyridinum afluorophosphate (OPFP); magnetic particles; p-nitrophenol (PNP); single-walled carbon nanotubes; tetrodotoxin (TTX)