title: Rescue of hypertension-related impairment of angiogenesis by therapeutic ultrasound.
abstract: We examined the hypothesis that therapeutic ultrasound (TUS) treatment would rescue the hypertension-related inhibition of ischemia-induced angiogenesis. TUS protects against endothelial dysfunction, but it is little known that the effect of TUS treatment on angiogenesis inhibited by hypertension. 20-week-old male spontaneously hypertensive rats (SHRs) and Wistar-Kyoto rats (WKYs) were randomly allocated to 4 groups: SHR; TUS treated SHR (SHR-TUS); WKY and TUS treated WKY (WKY-TUS). After undergoing excision of the left femoral artery, the ischemic skeletal muscles were treated with extracorporeal TUS for 9 minutes of daily exposure (frequency of 1 MHz, intensity of 0.3 W/cm(2)) for 14 consecutive days. We found that TUS normalized the blood perfusion in SHR-TUS accompanied by elevated capillary density. Similar results were found in the protein expression of angiogenic factors. TUS treatment also enhanced peripheral capillary density in WKY rats and restored the capillary rarefaction in hypertension by elevating the protein levels of endothelial nitric oxide synthase (eNOS), hypoxic inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and phosphorylated Akt (p-Akt) in vivo. Our data demonstrated that TUS treatment ameliorated hypertension-related inhibition of ischemia-induced angiogenesis, at least in part, via an NO-dependent manner.
authors: Department of Gerontology, Xinhua Hospital, Shanghai Jiaotong University School of MedicineKongjiang Road 1665, Shanghai 200092, China; Department of Vascular Surgery, Ren Ji Hospital, Shanghai Jiaotong University School of MedicineDongfang Road 1630, Shanghai 200127, China.
authors: Zhao-Yang
authors: ZY
authors: Lu
authors: Department of Gerontology, Xinhua Hospital, Shanghai Jiaotong University School of MedicineKongjiang Road 1665, Shanghai 200092, China; Department of Vascular Surgery, Ren Ji Hospital, Shanghai Jiaotong University School of MedicineDongfang Road 1630, Shanghai 200127, China.
authors: Rui-Lin
authors: RL
authors: Li
authors: Shanghai Acoustics Laboratory, Chinese Academy of Science Xiaomuqiao Road 456, Shanghai 200032, China.
authors: Hong-Sheng
authors: HS
authors: Zhou
authors: Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University Huaihai Xi Road 241, Xuhui District, Shanghai 200030, China.
authors: Jing-Juan
authors: JJ
authors: Huang
authors: Department of Gerontology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine Kongjiang Road 1665, Shanghai 200092, China.
authors: Jia
authors: J
authors: Qi
authors: Department of Gerontology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine Kongjiang Road 1665, Shanghai 200092, China.
authors: Zhi-Xiao
authors: ZX
authors: Su
authors: Department of Vascular Surgery, Ren Ji Hospital, Shanghai Jiaotong University School of Medicine Dongfang Road 1630, Shanghai 200127, China.
authors: Lan
authors: L
authors: Zhang
authors: Department of Internal Medicine, University of Iowa Carve College of Medicine 2000 Medical Laboratories 25 South Grand Avenue, Iowa City, IA 52242, USA.
authors: Yue
authors: Y
authors: Li
authors: Department of Nephrology Zhongshan Hospital, Fudan University Fenglin Road 180, Shanghai 200032, China.
authors: Yi-Qin
authors: YQ
authors: Shi
authors: Department of Vascular Surgery, Ren Ji Hospital, Shanghai Jiaotong University School of Medicine Dongfang Road 1630, Shanghai 200127, China.
authors: Chang-Ning
authors: CN
authors: Hao
authors: Department of Gerontology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine Kongjiang Road 1665, Shanghai 200092, China.
authors: Jun-Li
authors: JL
authors: Duan
keywords: Therapeutic ultrasound; angiogenesis; hindlimb ischemia; spontaneously hypertensive rat