title: Comprehensive two-dimensional liquid chromatography for polyphenol analysis in foodstuffs.
abstract: Polyphenols are a class of plant secondary metabolites that are recently drawing a special interest because of their broad spectrum of pharmacological effects. As they are characterized by an enormous structural variability, the identification of these molecules in food samples is a difficult task, and sometimes having only a limited number of commercially available reference materials is not of great help. One-dimensional liquid chromatography is the most widely applied analytical approach for their analysis. In particular, the hyphenation of liquid chromatography to mass spectrometry has come to play an influential role by allowing relatively fast tentative identification and accurate quantification of polyphenolic compounds at trace levels in vegetable media. However, when dealing with very complex real-world food samples, a single separation system often does not provide sufficient resolving power for attaining rewarding results. Comprehensive two-dimensional liquid chromatography is a technique of great analytical impact, since it offers much higher peak capacities than separations in a single dimension. In the present review, we describe applications in the field of comprehensive two-dimensional liquid chromatography for polyphenol analysis in real-world food samples. Comprehensive two-dimensional liquid chromatography applications to nonfood matrices fall outside the scope of the current report and will not be discussed.
authors: Dipartimento di "Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali,", University of Messina, Messina, Italy.
authors: Francesco
authors: F
authors: Cacciola
authors: Diabetes Research Institute, Division of Cellular Transplantation of Surgery, University of Miami, Miami, FL, USA.
authors: Sara
authors: S
authors: Farnetti
authors: Dipartimento di "Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Messina, Italy.|Chromaleont S.r.L, Viale Boccetta 70, 98122, Messina, Italy.|Unit of Food Science and Nutrition, Department of Medicine, University Campus Bio-Medico of Rome, Rome, Italy.
authors: Paola
authors: P
authors: Dugo
authors: Australian Centre of Research on Separation Science, School of Chemistry, Monash University, Clayton, Australia.
authors: Philip John
authors: PJ
authors: Marriott
authors: Dipartimento di "Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Messina, Italy.|Chromaleont S.r.L, Viale Boccetta 70, 98122, Messina, Italy.|Unit of Food Science and Nutrition, Department of Medicine, University Campus Bio-Medico of Rome, Rome, Italy.
authors: Luigi
authors: L
authors: Mondello
mesh_terms: D002853:Chromatography, Liquid; D005504:Food Analysis; D059808:Polyphenols / Q000032:analysis*
chemical_list: D059808:Polyphenols
keywords: Comprehensive two-dimensional liquid chromatography; Food; Mass spectrometry; Polyphenols