title: Distinct gene expression profile of Xanthomonas retroflexus engaged in synergistic multispecies biofilm formation.
abstract: It is well known that bacteria often exist in naturally formed multispecies biofilms. Within these biofilms, interspecies interactions seem to have an important role in ecological processes. Little is known about the effects of interspecies interactions on gene expression in these multispecies biofilms. This study presents a comparative gene expression analysis of the Xanthomonas retroflexus transcriptome when grown in a single-species biofilm and in dual- and four-species consortia with Stenotrophomonas rhizophila, Microbacterium oxydans and Paenibacillus amylolyticus. The results revealed complex interdependent interaction patterns in the multispecies biofilms. Many of the regulated functions are related to interactions with the external environment and suggest a high phenotypic plasticity in response to coexistence with other species. Furthermore, the changed expression of genes involved in aromatic and branched-chain amino acid biosynthesis suggests nutrient cross feeding as a contributing factor for the observed synergistic biofilm production when these four species coexists in a biofilm.
authors: Department of Biology, Section of Microbiology, University of Copenhagen, Copenhagen, Denmark.|Department of Systems Biology, Center for Biological Sequence Analysis, Technical University of Denmark, Kongens Lyngby, Denmark.
authors: Lea Benedicte Skov
authors: LB
authors: Hansen
authors: Department of Biology, Section of Microbiology, University of Copenhagen, Copenhagen, Denmark.
authors: Dawei
authors: D
authors: Ren
authors: Department of Biology, Section of Microbiology, University of Copenhagen, Copenhagen, Denmark.
authors: Mette
authors: M
authors: Burmølle
authors: Department of Biology, Section of Microbiology, University of Copenhagen, Copenhagen, Denmark.
authors: Søren J
authors: SJ
authors: Sørensen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms; D015964:Gene Expression Regulation, Bacterial; D059467:Transcriptome; D014974:Xanthomonas / Q000145:classification / Q000235:genetics / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins