title: Rheopathologic Consequence of Plasmodium vivax Rosette Formation.
abstract: Malaria parasites dramatically alter the rheological properties of infected red blood cells. In the case of Plasmodium vivax, the parasite rapidly decreases the shear elastic modulus of the invaded RBC, enabling it to avoid splenic clearance. This study highlights correlation between rosette formation and altered membrane deformability of P. vivax-infected erythrocytes, where the rosette-forming infected erythrocytes are significantly more rigid than their non-rosetting counterparts. The adhesion of normocytes to the PvIRBC is strong (mean binding force of 440pN) resulting in stable rosette formation even under high physiological shear flow stress. Rosetting may contribute to the sequestration of PvIRBC schizonts in the host microvasculature or spleen.
authors: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore.
authors: Rou
authors: R
authors: Zhang
authors: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore.
authors: Wenn-Chyau
authors: WC
authors: Lee
authors: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors: Yee-Ling
authors: YL
authors: Lau
authors: Laboratory of Tropical Diseases, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas-SP, Brazil.
authors: Letusa
authors: L
authors: Albrecht
authors: Laboratory of Tropical Diseases, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas-SP, Brazil.
authors: Stefanie C P
authors: SC
authors: Lopes
authors: Laboratory of Tropical Diseases, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas-SP, Brazil.
authors: Fabio T M
authors: FT
authors: Costa
authors: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore.
authors: Rossarin
authors: R
authors: Suwanarusk
authors: Shoklo Malaria Research Unit, Mahidol-Oxford tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, MaeSot, Thailand.|Centre for Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
authors: Francois
authors: F
authors: Nosten
authors: Programs in Infection and Immunity and Cardiovascular Disease, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Victoria, Australia.
authors: Brian M
authors: BM
authors: Cooke
authors: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore.
authors: Laurent
authors: L
authors: Rénia
authors: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore.|Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
authors: Bruce
authors: B
authors: Russell
mesh_terms: D000818:Animals; D055119:Elastic Modulus; D004907:Erythrocyte Deformability; D004910:Erythrocyte Membrane / Q000502:physiology*; D004912:Erythrocytes / Q000469:parasitology* / Q000473:pathology* / Q000502:physiology / Q000648:ultrastructure; D018056:Hemorheology; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood / Q000469:parasitology; D046210:Microfluidic Analytical Techniques; D010966:Plasmodium vivax / Q000276:immunology / Q000472:pathogenicity*; D012397:Rosette Formation; D013154:Spleen / Q000098:blood supply / Q000378:metabolism / Q000469:parasitology