title: Hybrid materials for bone tissue engineering from biomimetic growth of hydroxiapatite on cellulose nanowhiskers.
abstract: Cellulose nanowhiskers (CNWs) with different surface composition were used to generate the biomimetic growth hydroxyapatite (HAp). Hybrids materials primarily consist of CNWs with HAp content below 24%. CNWs were produced by different inorganic acid hydrolyses to generate cellulose particles with surface groups to induce HAp mineralization. In the present study, we evaluate the use of CNWs prepared from hydrochloric acid, sulfuric acid and phosphoric acid. HAp growth was obtained from the biomimetic method using a simulated body fluid concentration of 1.5M (SBF). The sulfonate and phosphonate groups on the CNW surface have a direct impact on the nucleation and growth of HAp. HAp/CNW were also compared with the physical mixture method using HAp nanoparticles prepared by chemical precipitation. The bioactivity and biocompatibility of the hybrid materials were assessed by cell viability studies using fibroblast cells (L929). The materials obtained from the biomimetic method have superior biocompatibility/bioactivity compared to the material synthesized by the wet chemical precipitation method with an incubation period of 24h.
authors: Departamento de Química, Universidade Estadual de Maringá, Av. Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil.
authors: Elizângela H
authors: EH
authors: Fragal
authors: Departamento de Química, Universidade Estadual de Maringá, Av. Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil.
authors: Thelma S P
authors: TSP
authors: Cellet
authors: Departamento de Química, Universidade Estadual de Maringá, Av. Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil.
authors: Vanessa H
authors: VH
authors: Fragal
authors: Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Av. Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil.
authors: Mychelle V P
authors: MVP
authors: Companhoni
authors: Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Av. Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil; Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Av. Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil.
authors: Tânia
authors: T
authors: Ueda-Nakamura
authors: Departamento de Química, Universidade Estadual de Maringá, Av. Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil; Programa de Pós-graduação em Biotecnologia Aplicada à Agricultura, Universidade Paranaense (UNIPAR), 87502-210, Umuarama-PR, Brazil; Programa de Pós-graduação em Ciência e Engenharia de Materiais, Universidade Tecnológica Federal do Paraná - Paraná (UTFPR-LD), 86036-370, Londrina-PR, Brazil.
authors: Edvani C
authors: EC
authors: Muniz
authors: Departamento de Química, Universidade Estadual de Maringá, Av. Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil. Electronic address: rafael_dqi@yahoo.com.br.
authors: Rafael
authors: R
authors: Silva
authors: Departamento de Química, Universidade Estadual de Maringá, Av. Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil. Electronic address: afrubira@uem.br.
authors: Adley F
authors: AF
authors: Rubira
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry*; D002460:Cell Line; D002482:Cellulose / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D023822:Tissue Engineering
chemical_list: D018786:Bone Substitutes; D002482:Cellulose; D017886:Durapatite
keywords: Biomimetic growth; Bone regeneration; Cellulose nanowhiskers; Hybrid material; Hydroxyapatite; Surface modification