title: Down-regulation of 21A Alu RNA as a tool to boost proliferation maintaining the tissue regeneration potential of progenitor cells.
abstract: 21A is an Alu non-coding (nc) RNA transcribed by RNA polymerase (pol) III. While investigating the biological role of 21A ncRNA we documented an inverse correlation between its expression level and the rate of cell proliferation. The downregulation of this ncRNA not only caused a boost in cell proliferation, but was also associated to a transient cell dedifferentiation, suggesting a possible involvement of this RNA in cell dedifferentiation/reprogramming. In this study, we explored the possibility to enhance proliferation and dedifferentiation of cells of interest, by 21A down-regulation, using a mixture of chemically modified Anti-21A RNAs. Our results confirmed the validity of this approach that allows the amplification of specific cell populations, in a controlled manner and without inducing permanent effects. In addition to induce cell proliferation, the procedure did not decrease the tissue regeneration potential of progenitor cells in two different cell systems.
authors: a Dept. of Experimental Medicine (DIMES) , University of Genova , Genova , Italy.
authors: Arianna
authors: A
authors: Gigoni
authors: b IRCCS-AOU San Martino-IST , Genova , Italy.
authors: Delfina
authors: D
authors: Costa
authors: c ISMETT, Mediterranean Institute for Transplantation and Advanced Specialized Therapies , Palermo , Italy.|d Ri.MED Foundation , Palermo , Italy.
authors: Massimiliano
authors: M
authors: Gaetani
authors: a Dept. of Experimental Medicine (DIMES) , University of Genova , Genova , Italy.|b IRCCS-AOU San Martino-IST , Genova , Italy.
authors: Roberta
authors: R
authors: Tasso
authors: a Dept. of Experimental Medicine (DIMES) , University of Genova , Genova , Italy.
authors: Federico
authors: F
authors: Villa
authors: e Sect. of Pharmacology, Dept. of Internal Medicine (DiMI) and Center of Excellence for Biomedical Research (CEBR), University of Genova , Genova , Italy.
authors: Tullio
authors: T
authors: Florio
authors: a Dept. of Experimental Medicine (DIMES) , University of Genova , Genova , Italy.|b IRCCS-AOU San Martino-IST , Genova , Italy.
authors: Aldo
authors: A
authors: Pagano
mesh_terms: D000293:Adolescent; D000328:Adult; D020087:Alu Elements / Q000235:genetics*; D000818:Animals; D002113:Calcification, Physiologic; D063646:Carcinogenesis / Q000473:pathology; D054337:Cell Dedifferentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002828:Choristoma / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000235:genetics*; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D018919:Neovascularization, Physiologic; D009447:Neuroblastoma / Q000473:pathology; D010012:Osteogenesis; D016372:RNA, Antisense / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D012038:Regeneration; D014162:Transfection; D055815:Young Adult
chemical_list: D016372:RNA, Antisense; D022661:RNA, Untranslated
keywords: Alu; antisense RNAs; cell proliferation; differentiation; mesenchimal stem cells; non-coding RNAs