title: Human and Mouse Brown Adipose Tissue Mitochondria Have Comparable UCP1 Function.
abstract: Brown adipose tissue (BAT) plays an important role in mammalian thermoregulation. The component of BAT mitochondria that permits this function is the inner membrane carrier protein uncoupling protein 1 (UCP1). To the best of our knowledge, no studies have directly quantified UCP1 function in human BAT. Further, whether human and rodent BAT have comparable thermogenic function remains unknown. We employed high-resolution respirometry to determine the respiratory capacity, coupling control, and, most importantly, UCP1 function of human supraclavicular BAT and rodent interscapular BAT. Human BAT was sensitive to the purine nucleotide GDP, providing the first direct evidence that human BAT mitochondria have thermogenically functional UCP1. Further, our data demonstrate that human and rodent BAT have similar UCP1 function per mitochondrion. These data indicate that human and rodent BAT are qualitatively similar in terms of UCP1 function.
authors: Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, Galveston, TX 77550, USA. Electronic address: cr2porte@utmb.edu.
authors: Craig
authors: C
authors: Porter
authors: Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, Galveston, TX 77550, USA.
authors: David N
authors: DN
authors: Herndon
authors: Department of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, Galveston, TX 77550, USA.
authors: Maria
authors: M
authors: Chondronikola
authors: Department of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, Galveston, TX 77550, USA.
authors: Tony
authors: T
authors: Chao
authors: Department of Radiology, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Palam
authors: P
authors: Annamalai
authors: Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, Galveston, TX 77550, USA.
authors: Nisha
authors: N
authors: Bhattarai
authors: Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, Galveston, TX 77550, USA.
authors: Manish K
authors: MK
authors: Saraf
authors: Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, Galveston, TX 77550, USA.
authors: Karel D
authors: KD
authors: Capek
authors: Department of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Paul T
authors: PT
authors: Reidy
authors: The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
authors: Alexes C
authors: AC
authors: Daquinag
authors: The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
authors: Mikhail G
authors: MG
authors: Kolonin
authors: Department of Nutrition and Metabolism, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Blake B
authors: BB
authors: Rasmussen
authors: Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA; Department of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Elisabet
authors: E
authors: Borsheim
authors: Department of Anesthesiology, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Tracy
authors: T
authors: Toliver-Kinsky
authors: Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA; Shriners Hospitals for Children, Galveston, Galveston, TX 77550, USA.
authors: Labros S
authors: LS
authors: Sidossis
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism* / Q000648:ultrastructure; D052436:Adipose Tissue, White / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D019069:Cell Respiration; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000378:metabolism; D009333:Neck; D000071256:Uncoupling Protein 1 / Q000378:metabolism*
chemical_list: D000071256:Uncoupling Protein 1