title: Investigation of enteropathogenic Escherichia coli and Shiga toxin-producingEscherichia coli associated with hemolytic uremic syndrome in İzmir Province, Turkey.
abstract: OBJECTIVE
  The purpose of this study was to investigate Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) strains originating from diarrheagenic patients.
  
  
  METHODS
  A total of 102 patients with diarrhea between October 2012 and January 2013 were enrolled in this study. Multiplex and standard polymerase chain reactions were performed to detect and distinguish STEC and EPEC strains. O serotyping of EPEC was carried out by monovalent antisera. The O and H serotyping of STEC strains was performed at the Refik Saydam Institute, Ankara.
  
  
  RESULTS
  A total of 5 (3.42%) strains were identified as STEC, and 3 strains (2.05%) were atypical EPEC. One of the STEC serotypes was O157:H7 carrying VT1, Stx1A, and escv genes. The other STEC strain was identified as O174:H21, which is associated with hemolytic uremic syndrome and consists of VT2 and Stx2A genes. One of the EPEC and three of the STEC serotypes were nontypeable. The serotypes of the atypical EPEC strains were identified as O114 and O26.
  
  
  CONCLUSIONS
  To the best of our knowledge, this is the first report of O174:H21 from the İzmir region that was shown to be a Shiga toxin-producing non-O157 serotype of STEC.
authors: Department of Biology, Basic and Industrial Microbiology Section, Faculty of Science, Ege University, İzmir, Turkey.
authors: Elif
authors: E
authors: Bozçal
authors: Department of Biology, Basic and Industrial Microbiology Section, Faculty of Science, Ege University, İzmir, Turkey.
authors: Gürkan
authors: G
authors: Yiğittürk
authors: Department of Biology, Basic and Industrial Microbiology Section, Faculty of Science, Ege University, İzmir, Turkey.
authors: Ataç
authors: A
authors: Uzel
authors: Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Ege University, İzmir, Turkey.
authors: Sabire Şöhret
authors: SŞ
authors: Aydemir
mesh_terms: D018829:Adhesins, Bacterial; D054308:Enteropathogenic Escherichia coli; D004927:Escherichia coli Infections; D029968:Escherichia coli Proteins; D006463:Hemolytic-Uremic Syndrome; D006801:Humans; D022621:Shiga Toxin; D014421:Turkey; D014774:Virulence
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D022621:Shiga Toxin
keywords: Enteropathogenic Escherichia coli; MALDI-TOF mass spectrometry; Shiga toxin-producing Escherichia coli; diarrhea; polymerase chain reaction; serotyping