title: The nucleolytic resolution of recombination intermediates in yeast mitotic cells.
abstract: In mitotic cells, the repair of double-strand breaks by homologous recombination (HR) is important for genome integrity. HR requires the orchestration of a subset of pathways for timely removal of joint-molecule intermediates that would otherwise prevent segregation of chromosomes in mitosis. The use of nucleases to resolve recombination intermediates is important for chromosome segregation, but is hazardous because crossovers can result in loss of heterozygosity or chromosome rearrangements. Unregulated use of the nucleases involved in the resolution of recombination intermediates could also be a risk during replication. The yeast models (Saccharomyces cerevisae and Schizosaccharomyces pombe) have proven effective in determining the major nucleases involved in the processing of such intermediates: Mus81-Mms4 and Yen1. Mus81-Mms4 and Yen1 are regulated by the cell cycle in a gradual activation during G2/M to keep the crossing-over risk low while ensuring proper removal of HJ intermediates.
authors: Université Paris-Saclay, Centre National de la Recherche Scientifique (CNRS) UMR 8200 Genetic Stability and Oncogenesis, Gustave Roussy, 114 rue Edouard Vaillant, 94805 Villejuif, France.
authors: Ibtissam
authors: I
authors: Talhaoui
authors: Université Paris-Saclay, Centre National de la Recherche Scientifique (CNRS) UMR 8200 Genetic Stability and Oncogenesis, Gustave Roussy, 114 rue Edouard Vaillant, 94805 Villejuif, France.
authors: Manuel
authors: M
authors: Bernal
authors: Université Paris-Saclay, Centre National de la Recherche Scientifique (CNRS) UMR 8200 Genetic Stability and Oncogenesis, Gustave Roussy, 114 rue Edouard Vaillant, 94805 Villejuif, France gerard.mazon@gustaveroussy.fr.
authors: Gerard
authors: G
authors: Mazón
mesh_terms: D004271:DNA, Fungal / Q000235:genetics* / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism*; D059765:Homologous Recombination; D008938:Mitosis; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development
chemical_list: D004271:DNA, Fungal; D004720:Endonucleases
keywords: Mus81-Mms4, Yen1; double-strand break repair; homologous recombination; nucleolytic resolution; structure-selective nucleases