title: Increased expression levels of ppGalNAc-T13 in lung cancers: Significance in the prognostic diagnosis.
abstract: ppGalNAc-T13 is upregulated along with reduced expression of GM1 in high metastatic sublines of the murine Lewis lung cancer cell line, but little is known about the implication of ppGalNAc-T13 expression in human cancers. Since lung cancer cell lines showed high expression levels of ppGalNAc-T13, we analyzed ppGalNAc-T13 expression in surgical lung cancer specimens to examine whether ppGalNAc-T13 can be used as a prognostic marker or a therapeutic target. We analyzed mRNA expression levels of GALNT13 and its variant exon usages in surgical specimens by real-time RT-PCR, and the results were evaluated by correlating with clinical data. Ninety-one surgical specimens were analyzed. Consequently, recurrence-free survival was significantly shorter (P=0.045) in high expression group of GALNT13 mRNA. In the analysis of tumor specific exon usage in GALNT13 RNA sequence, one variant exon was significantly associated with worse prognosis. By contrast, in another variant exon, positive variant expression group showed better prognosis than negative group. We also tried to detect GALNT13 mRNA in 63 serum samples from patients with lung cancers to examine whether GALNT13 mRNA can be measured in body fluids, detecting significant levels in 4 samples. Finally, expression of GM1, ppGalNAc-T13 and trimeric Tn antigen was examined by immunohistochemistry in order to evaluate them as a prognostic factor. It was demonstrated that ppGalNAc-T13 and trimeric Tn antigen had a relationship with worse prognosis in 35 investigated lung cancer patients. In conclusion, our results suggest that ppGalNAc-T13 might be a useful prognostic factor of lung cancers.
authors: Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Kenichi
authors: K
authors: Nogimori
authors: Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Tomoko
authors: T
authors: Hori
authors: Department of Thoracic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Koji
authors: K
authors: Kawaguchi
authors: Department of Thoracic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Takayuki
authors: T
authors: Fukui
authors: Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Shinji
authors: S
authors: Mii
authors: Department of Molecular Bioscience, Faculty of Life Sciences, Kyoto Sangyo University, Kyoto, Japan.
authors: Hiroshi
authors: H
authors: Nakada
authors: Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Yasuyuki
authors: Y
authors: Matsumoto
authors: Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Yoshio
authors: Y
authors: Yamauchi
authors: Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Masahide
authors: M
authors: Takahashi
authors: Faculty of Life and Health Sciences, Chubu University, Kasugai, Japan.
authors: Keiko
authors: K
authors: Furukawa
authors: Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Okajima
authors: O
authors: Tetsuya
authors: Department of Thoracic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Kohei
authors: K
authors: Yokoi
authors: Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Yoshinori
authors: Y
authors: Hasegawa
authors: Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Koichi
authors: K
authors: Furukawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D005091:Exons; D005260:Female; D005732:Gangliosides / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D014644:Genetic Variation; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics* / Q000378:metabolism*; D011379:Prognosis; D015854:Up-Regulation
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D005732:Gangliosides; C040286:Tn antigen; D017350:N-Acetylgalactosaminyltransferases; C000589774:GALNT13 protein, human