title: Identification of vaginal bacteria diversity and it's association with clinically diagnosed bacterial vaginosis by denaturing gradient gel electrophoresis and correspondence analysis.
abstract: Bacterial vaginosis (BV) is a common complex associated with numerous adverse health outcomes, affecting women of different ages throughout the world. The etiology of BV remains poorly understood due to the difficulty of establishing a molecular genetic criterion to recognize the vaginal microbiota of BV-positive women from that of the normal women. We used techniques of broad-range PCR-DGGE and gel imaging analysis system cooperated with 16S rRNA gene sequencing and statistical analysis to investigate the community structure of the healthy and BV-affected vaginal microbial ecosystems. The community of vaginal bacteria detected in subjects with BV was far more luxuriant and diverse than in subjects without BV. The mean number of microbial species in 128 BV-positive women was nearly two times greater than in 68 subjects without BV(4.05±1.96 versus 2.59±1.14). Our sequencing efforts yielded many novel phylotypes (198 of our sequences represented 59 species), including several novel BV-associated bacteria (BVAB) and many belonging to opportunistic infections, which remain inexplicable for their roles in determining the health condition of vaginal microflora. This study identifies Algoriphagus aquatilis, Atopobium vaginae, Burkholderia fungorum, Megasphaera genomosp species as indicators to BV and subjects with BV harbor particularly taxon-rich and diverse bacterial communities. Maybe Bifidobacterium, Staphylococcus or even more alien species are commensal creatures in normal vaginal microbiota.
authors: Qilu Hospital of Shandong University, Jinan, China.
authors: Qing
authors: Q
authors: Xia
authors: Second Affiliated Hospital, Shandong University of Traditional Chinese Medicine, Jinan, China.
authors: Lijuan
authors: L
authors: Cheng
authors: Institute of Basic Medicine of Shandong Academy of Medical Sciences, Jinan, China.
authors: Hua
authors: H
authors: Zhang
authors: Institute of Basic Medicine of Shandong Academy of Medical Sciences, Jinan, China.
authors: Shangwen
authors: S
authors: Sun
authors: Second Affiliated Hospital, Shandong University of Traditional Chinese Medicine, Jinan, China.
authors: Fang
authors: F
authors: Liu
authors: Qilu Hospital of Shandong University, Jinan, China.
authors: Hao
authors: H
authors: Li
authors: Jinan Military General Hospital, Jinan, China.
authors: Jing
authors: J
authors: Yuan
authors: Institute of Basic Medicine of Shandong Academy of Medical Sciences, Jinan, China.
authors: Zhendong
authors: Z
authors: Liu
authors: Institute of Basic Medicine of Shandong Academy of Medical Sciences, Jinan, China. Electronic address: dyutium@sina.com.
authors: Yutao
authors: Y
authors: Diao
mesh_terms: D000293:Adolescent; D000328:Adult; D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D044822:Biodiversity; D004269:DNA, Bacterial; D058645:Denaturing Gradient Gel Electrophoresis; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008875:Middle Aged; D015995:Prevalence; D016585:Vaginosis, Bacterial / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology*; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial
keywords: Bacterial vaginosis (BV); Clone library analysis; Correspondence analysis; Gel imaging analysis system; PCR-DGGE