title: "Race for the Surface": Eukaryotic Cells Can Win.
abstract: With an aging population and the consequent increasing use of medical implants, managing the possible infections arising from implant surgery remains a global challenge. Here, we demonstrate for the first time that a precise nanotopology provides an effective intervention in bacterial cocolonization enabling the proliferation of eukaryotic cells on a substratum surface, preinfected by both live Gram-negative, Pseudomonas aeruginosa, and Gram-positive, Staphylococcus aureus, pathogenic bacteria. The topology of the model black silicon (bSi) substratum not only favors the proliferation of eukaryotic cells but is biocompatible, not triggering an inflammatory response in the host. The attachment behavior and development of filopodia when COS-7 fibroblast cells are placed in contact with the bSi surface are demonstrated in the dynamic study, which is based on the use of real-time sequential confocal imaging. Bactericidal nanotopology may enhance the prospect for further development of inherently responsive antibacterial nanomaterials for bionic applications such as prosthetics and implants.
authors: School of Science, Swinburne University of Technology , P.O. Box 218, Hawthorn, Victoria 3122, Australia.
authors: Vy T H
authors: VT
authors: Pham
authors: School of Science, Swinburne University of Technology , P.O. Box 218, Hawthorn, Victoria 3122, Australia.
authors: Vi Khanh
authors: VK
authors: Truong
authors: Frankfurt Orofacial Regenerative Medicine, University Hospital Frankfurt , Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany.
authors: Anna
authors: A
authors: Orlowska
authors: Frankfurt Orofacial Regenerative Medicine, University Hospital Frankfurt , Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany.
authors: Shahram
authors: S
authors: Ghanaati
authors: Frankfurt Orofacial Regenerative Medicine, University Hospital Frankfurt , Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany.
authors: Mike
authors: M
authors: Barbeck
authors: Frankfurt Orofacial Regenerative Medicine, University Hospital Frankfurt , Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany.
authors: Patrick
authors: P
authors: Booms
authors: Monash Micro Imaging, Monash University , Clayton, Victoria 3800, Australia.
authors: Alex J
authors: AJ
authors: Fulcher
authors: School of Science, Swinburne University of Technology , P.O. Box 218, Hawthorn, Victoria 3122, Australia.
authors: Chris M
authors: CM
authors: Bhadra
authors: School of Science, Swinburne University of Technology , P.O. Box 218, Hawthorn, Victoria 3122, Australia.
authors: Ričardas
authors: R
authors: Buividas
authors: Departament d'Enginyeria Quimica, Universitat Rovira i Virgili , 26 Avenue dels Paisos Catalans, Tarragona 43007, Spain.
authors: Vladimir
authors: V
authors: Baulin
authors: Frankfurt Orofacial Regenerative Medicine, University Hospital Frankfurt , Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany.
authors: C James
authors: CJ
authors: Kirkpatrick
authors: School of Science, Swinburne University of Technology , P.O. Box 218, Hawthorn, Victoria 3122, Australia.
authors: Pauline
authors: P
authors: Doran
authors: School of Science, Swinburne University of Technology , P.O. Box 218, Hawthorn, Victoria 3122, Australia.
authors: David E
authors: DE
authors: Mainwaring
authors: School of Science, Swinburne University of Technology , P.O. Box 218, Hawthorn, Victoria 3122, Australia.
authors: Saulius
authors: S
authors: Juodkazis
authors: School of Science, Swinburne University of Technology , P.O. Box 218, Hawthorn, Victoria 3122, Australia.|School of Science, RMIT University , P.O. Box 2476, Melbourne, Victoria 3001, Australia.
authors: Russell J
authors: RJ
authors: Crawford
authors: School of Science, Swinburne University of Technology , P.O. Box 218, Hawthorn, Victoria 3122, Australia.
authors: Elena P
authors: EP
authors: Ivanova
mesh_terms: D000900:Anti-Bacterial Agents; D005057:Eukaryotic Cells; D049329:Nanostructures; D011550:Pseudomonas aeruginosa; D013211:Staphylococcus aureus; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents
keywords: bactericidal surfaces; competitive colonization; inflammatory response; microbial contamination; nanostructures