title: Paraburkholderia caffeinitolerans sp. nov., a caffeine degrading species isolated from a tea plantation soil sample.
abstract: A Gram-stain negative, facultatively anaerobic, non-sporulating, non-motile and rod-shaped bacterium, designated CF3T, was isolated from a tea plantation soil sample and its taxonomic position was determined using polyphasic taxonomy. Strain CF3Tdisplayed optimum growth at 25 °C, pH 5.0 and in the presence of 0-1 % NaCl. Comparative phylogenetic analysis of the 16S rRNA, recA and gyrB gene sequences showed that the isolate belongs to the genus Paraburkholderia, showing high levels of similarity with respect to Paraburkholderia oxyphila OX-01T(98.3, 95 and 93 %, respectively) and Paraburkholderia sacchari IPT101T(98.2, 95 and 95 %, respectively). The predominant ubiquinone was determined to be Q-8, and the polar lipids are phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified amino-phospholipid, three unidentified amino-lipids and three unidentified polar lipids. The major fatty acids were found to be C16:0, C17:0 cyclo, summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The DNA G+C content was found to be 63.8 mol% and the DNA-DNA relatedness values between strain CF3Tand its two close relatives P. oxyphila OX-01Tand P. sacchari IPT101Twas 41 and 40 %, respectively. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate represents a novel species of the genus Paraburkholderia, for which the name Paraburkholderia caffeinitolerans sp. nov. is proposed. The type strain is CF3T(= LMG 28688T= CGMCC 1.15105T).
authors: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China.
authors: Zi-Qing
authors: ZQ
authors: Gao
authors: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China.
authors: Dong-Ying
authors: DY
authors: Zhao
authors: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China.
authors: Lei
authors: L
authors: Xu
authors: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China.
authors: Rui-Ting
authors: RT
authors: Zhao
authors: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China.
authors: Ming
authors: M
authors: Chen
authors: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China. zhangcz@dlpu.edu.cn.
authors: Chun-Zhi
authors: CZ
authors: Zhang
mesh_terms: D000383:Agriculture; D001482:Base Composition; D042521:Burkholderiaceae / Q000302:isolation & purification* / Q000378:metabolism; D002110:Caffeine / Q000378:metabolism*; D028241:Camellia sinensis; D004269:DNA, Bacterial; D058889:Molecular Typing; D010802:Phylogeny; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002110:Caffeine
keywords: Caffeine degradation; Paraburkholderia caffeinitolerans sp. nov.; Polyphasic taxonomy