title: Analysis and quality control of carbohydrates in therapeutic proteins with fluorescence HPLC.
abstract: Conbercept is an Fc fusion protein with very complicated carbohydrate profiles which must be carefully monitored through manufacturing process. Here, we introduce an optimized fluorescence derivatization high-performance liquid chromatographic method for glycan mapping in conbercept. Compared with conventional glycan analysis method, this method has much better resolution and higher reproducibility making it excellent for product quality control.
authors: Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Kun
authors: K
authors: Liu
authors: Key Laboratory for NeuroInformation of Ministry of Education, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China; Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors: Jian
authors: J
authors: Huang
authors: Chengdu Nuoen Biotechnologies, LTD, Chengdu, China.
authors: Delun
authors: D
authors: Luo
authors: Chengdu Nuoen Biotechnologies, LTD, Chengdu, China.
authors: Kai
authors: K
authors: Xu
authors: Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China; Chengdu Nuoen Biotechnologies, LTD, Chengdu, China. Electronic address: wubox@yahoo.com.
authors: Zhigang
authors: Z
authors: Wu
authors: Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China. Electronic address: drxuxun@tom.com.
authors: Xun
authors: X
authors: Xu
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D002021:Buffers; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006031:Glycosylation; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry* / Q000302:isolation & purification; D010449:Peptide Mapping / Q000379:methods*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000737:chemistry; D011786:Quality Control; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000302:isolation & purification; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods*; D062367:ortho-Aminobenzoates / Q000737:chemistry
chemical_list: D020533:Angiogenesis Inhibitors; D002021:Buffers; D007141:Immunoglobulin Fc Fragments; D011993:Recombinant Fusion Proteins; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; C527363:KH902 fusion protein; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
keywords: Carbohydrates; Conbercept; Fc fusion protein; Fluorescence HPLC; Glycan mapping