title: Characterization and quantification of monoterpenoids in different types of peony root and the related Paeonia species by liquid chromatography coupled with ion trap and time-of-flight mass spectrometry.
abstract: Monoterpenoids with "cage-like" pinane skeleton are the unique and main bioactive constituents in peony root, the root of Paeonia lactiflora. A liquid chromatography coupled with ion trap and time-of-flight mass spectrometry (LC-IT-TOF-MS) method was developed for characterization and quantification of monoterpenoids in different types of peony root and the roots of related Paeonia species. MS/MS fragmentation patterns of monoterpenoids with paeoniflorin-, albiflorin- and sulfonated paeoniflorin-type of skeletons were elucidated, which provided basic clues enabling subsequent identification of 35 monoterpenoids in LC-MS profiles of Paeonia species. The profiling analysis and further quantification of 15 main monoterpenoids in 56 samples belonged to red peony root (RPR), white peony root (WPR), peony root in Japanese market (PR) and the roots of related Paeonia species revealed that paeoniflorin, benzoylpaeoniflorin, galloylpaeoniflorin, oxypaoniflorin and albiflorin were predominant constituents in all the samples; mudanpioside C was the characteristic component of P. lactiflora, and 4-O-methyl-paeoniflorin was only detected in P. veitchii and P. anomala. Total contents of the 15 monoterpenoids were obviously higher in the roots of P. lactiflora and P. veitchii than in those of P. anomala and P. japonica. Principal component analysis based on the quantitative results showed that the samples derived from P. lactiflora were clearly classified into RPR, WPR/PR, and sulfur-fumigated WPR groups, besides the respective group of P. veitchii and P. anomala. This study clarified the chemical characteristics of the respective type of peony root and the related Paeonia species, as well as the marker constituents for their discrimination.
authors: Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, Toyama 9300194, Japan; The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors: Yan-Hong
authors: YH
authors: Shi
authors: Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, Toyama 9300194, Japan. Electronic address: szhu@inm.u-toyama.ac.jp.
authors: Shu
authors: S
authors: Zhu
authors: Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, Toyama 9300194, Japan.
authors: Yue-Wei
authors: YW
authors: Ge
authors: Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, Toyama 9300194, Japan.
authors: Kazufumi
authors: K
authors: Toume
authors: The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors: Zhengtao
authors: Z
authors: Wang
authors: School of Engineering and Applied Sciences, National University of Mongolia, Ulaanbaatar-13, Mongolia.
authors: Javzan
authors: J
authors: Batkhuu
authors: Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, Toyama 9300194, Japan. Electronic address: katsukok@inm.u-toyama.ac.jp.
authors: Katsuko
authors: K
authors: Komatsu
mesh_terms: D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry; D001952:Bridged-Ring Compounds / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry; D039821:Monoterpenes / Q000737:chemistry*; D029596:Paeonia / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods; D006027:Glycosides
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C119233:galloylpaeoniflorin; C015423:peoniflorin; D006027:Glycosides; C047591:benzoylpaeoniflorin; C014959:albiflorin
keywords: Fragmentation patterns; LC-IT-TOF-MS; Monoterpenoids profile; Paeonia lactiflora; Peony root; Quantitative analysis