title: Bioinspired double polysaccharides-based nanohybrid scaffold for bone tissue engineering.
abstract: The fabrication of bone scaffolds with interconnected porous structure, adequate mechanical properties and excellent biocompatibility presents a great challenge. Herein, a hybrid nanostructured chitosan/chondroitin sulfate/hydroxyapatite (ChS/CSA/HAP) in situ composite scaffold was prepared by in situ fabrication and freeze-drying technique. The composition and morphology of scaffold were characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM). It proved that the low crystallinity of HAP crystals were uniformly distributed in ChS/CSA organic matrix and the nanostructured hybrid scaffold exhibited good mechanical property. The biocompatibility and in vitro bioactivity were detected by MTT-assay, maturation (alkaline phosphatase (ALP) activity), Hoechst 33258 and PI fluorescence staining. In vitro tests indicated that the hybrid scaffold not only promoted the adhesion and proliferation of osteoblasts, but also improved the growth of the osteoblasts. Therefore, it is promising for bone repair application in bone tissue engineering.
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors: Tiantang
authors: T
authors: Fan
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China. Electronic address: ibptcjd@fzu.edu.cn.
authors: Jingdi
authors: J
authors: Chen
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors: Panpan
authors: P
authors: Pan
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors: Yujue
authors: Y
authors: Zhang
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors: Yimin
authors: Y
authors: Hu
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors: Xiaocui
authors: X
authors: Liu
authors: School of Material Science and Engineering, South China University of Technology, Guangzhou 510640, China.
authors: Xuetao
authors: X
authors: Shi
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China; Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192, China. Electronic address: zhangqiq@126.com.
authors: Qiqing
authors: Q
authors: Zhang
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D001842:Bone and Bones / Q000166:cytology*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D010006:Osteoblasts / Q000166:cytology*; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; C480355:chitosan sulfate; D048271:Chitosan; D017886:Durapatite
keywords: Chitosan; Chondroitin sulfate; In situ; Nano-hydroxyapatite