title: Original Research: Potential ocular protection and dynamic observation of Polygonatum sibiricum polysaccharide against streptozocin-induced diabetic rats' model.
abstract: Ocular complications associated with diabetes mellitus are progressive and becoming one of the most important causes of morbidity worldwide. The purpose of the study is to evaluate the protective effect of Polygonatum sibiricum polysaccharide, an important component of Polygonatum sibiricum, on ocular complications in streptozotocin-induced diabetes mellitus rats. Sprague Dawley rats were made diabetic with streptozotocin(60 mg/kg, i.v.) and then the rats were treated with Polygonatum sibiricum polysaccharide 200, 400 and 800 mg/kg.d by gavage for 12 weeks. Biochemical analysis indicated that Polygonatum sibiricum polysaccharide lowered the levels of fasting blood glucose and glycated hemoglobin in blood and elevated the levels of insulin and C-peptide in plasma of diabetes mellitus rats in a dose-dependent manner. Physical measurements revealed that Polygonatum sibiricum polysaccharide improved clinical symptoms of polydipsia, polyphagia, polyuria and weight loss in diabetes mellitus rats. The content of malondialdehyde and activity of superoxide dismutase in plasma were determined, and the data showed Polygonatum sibiricum polysaccharide suppressed oxidative stress reaction. Lens opacification was observed using slit lamp illumination, and the data showed Polygonatum sibiricum polysaccharide delayed cataract progression in a dose-dependent manner. Electroretinogram showed Polygonatum sibiricum polysaccharide treatment reversed the decrease of electroretinogram b and OPs2 waves' amplitudes. Flash-visual evoked potential test indicated that the peak time of P2 wave was prolonged, and the amplitude of N2-P2 was lowered in diabetes mellitus group, and Polygonatum sibiricum polysaccharide suppressed these changes. Fundus fluorescein angiography showed Polygonatum sibiricum polysaccharide alleviated the retinal vasculopathy in a dose-dependent manner. In conclusion, these results suggest that the administration of Polygonatum sibiricum polysaccharide slows the progression of diabetic retinopathy and cataract through alleviating hyperglycemia and reducing oxidative stress in streptozotocin-induced diabetes mellitus rats.
authors: Department of Ophthalmology, Chonbuk National University, Jeollabuk-do 561-756, Republic of Korea (past position).|Department of Ophthalmology, Affiliated Hospital of Taishan Medical University, Taian 271000, China.
authors: Yi
authors: Y
authors: Wang
authors: Key Laboratory of Atherosclerosis in Universities of Shandong and Institute of Atherosclerosis, Taishan Medical University, Taian 271000, China.
authors: Shucun
authors: S
authors: Qin
authors: Department of Ophthalmology, Affiliated Hospital of Taishan Medical University, Taian 271000, China.
authors: Guoqing
authors: G
authors: Pen
authors: Department of Ophthalmology, Affiliated Hospital of Taishan Medical University, Taian 271000, China.
authors: Di
authors: D
authors: Chen
authors: Department of Ophthalmology, Affiliated Hospital of Taishan Medical University, Taian 271000, China.
authors: Chao
authors: C
authors: Han
authors: Department of Ophthalmology, Affiliated Hospital of Taishan Medical University, Taian 271000, China.
authors: Chunrun
authors: C
authors: Miao
authors: Department of Ophthalmology, Affiliated Hospital of Taishan Medical University, Taian 271000, China.
authors: Baojin
authors: B
authors: Lu
authors: Department of Ophthalmology, Affiliated Hospital of Taishan Medical University, Taian 271000, China.
authors: Chao
authors: C
authors: Su
authors: Department of Ophthalmology, Affiliated Hospital of Taishan Medical University, Taian 271000, China.
authors: Shanlong
authors: S
authors: Feng
authors: Department of Ophthalmology, Affiliated Hospital of Taishan Medical University, Taian 271000, China.
authors: Wen
authors: W
authors: Li
authors: Department of Ophthalmology, Affiliated Hospital of Taishan Medical University, Taian 271000, China.
authors: Jingjing
authors: J
authors: Han
authors: Department of Ophthalmology, Chonbuk National University, Jeollabuk-do 561-756, Republic of Korea (past position) cnauo@jbnu.ac.kr.
authors: Nam C
authors: NC
authors: Cho
authors: Key Laboratory of Atherosclerosis in Universities of Shandong and Institute of Atherosclerosis, Taishan Medical University, Taian 271000, China zhaosixinkang@163.com.
authors: Yanhong
authors: Y
authors: Si
mesh_terms: D000818:Animals; D002386:Cataract / Q000188:drug therapy* / Q000209:etiology; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000188:drug therapy / Q000378:metabolism; D003930:Diabetic Retinopathy / Q000188:drug therapy; D004596:Electroretinography; D005074:Evoked Potentials, Visual / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D059606:Polydipsia / Q000188:drug therapy / Q000209:etiology; D031384:Polygonatum / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D011141:Polyuria / Q000188:drug therapy; D017207:Rats, Sprague-Dawley; D013311:Streptozocin
chemical_list: D005947:Glucose; D006442:Glycated Hemoglobin; D007004:Hypoglycemic Agents; D011134:Polysaccharides; D013311:Streptozocin
keywords: Polygonatum sibiricum polysaccharide; cataract; diabetes mellitus; diabetic retinopathy; ocular complications