title: miRNA-24-3p promotes cell proliferation and regulates chemosensitivity in head and neck squamous cell carcinoma by targeting CHD5.
abstract: OBJECTIVE
  To investigate the role of miR-24-3p in tumorigenesis and chemosensitivity in head and neck squamous cell carcinoma (HNSCC).
  
  
  METHODS
  Growth rate and colony formation assays were performed after transfection with miR-24-3p mimic and inhibitor in cultured SCC-15 cells, followed by a CellTiter-Glo®assay. Western blot and luciferase assays were performed to investigate the direct target of miR-24-3p. Xenograft mouse model was used to evaluate combinatorial effects of miR-24-3p inhibitor and 5-fluorouracil.
  
  
  CONCLUSIONS
  Inhibition of miR-24-3p reduced cell proliferation, colony formation efficiency and reversed chemoresistance in HNSCC cells. CHD5 is the direct target of miR-24-3p which is required for the regulatory role of miR-24-3p in chemoresistance. miR-24-3p may represent a new therapeutic target for the improvement of clinical outcome in HNSCC.
authors: Department of Stomatology, The Second People's Hospital of Wuxi, 68 Zhong Shan Road, Wuxi 214002, Jiangsu, PR China.
authors: Xiaofeng
authors: X
authors: Sun
authors: Department of Otolaryngology, The Second People's Hospital of Wuxi, 68 Zhong Shan Road, Wuxi 214002, Jiangsu, PR China.
authors: Dajiang
authors: D
authors: Xiao
authors: Department of Otolaryngology, The Second People's Hospital of Wuxi, 68 Zhong Shan Road, Wuxi 214002, Jiangsu, PR China.
authors: Ting
authors: T
authors: Xu
authors: Department of Otolaryngology, The Second People's Hospital of Wuxi, 68 Zhong Shan Road, Wuxi 214002, Jiangsu, PR China.
authors: Yuan
authors: Y
authors: Yuan
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004265:DNA Helicases / Q000235:genetics*; D004195:Disease Models, Animal; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005472:Fluorouracil / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics*; D034622:RNA Interference; D000077195:Squamous Cell Carcinoma of Head and Neck; D014410:Tumor Stem Cell Assay; D023041:Xenograft Model Antitumor Assays
chemical_list: D020413:3' Untranslated Regions; D000970:Antineoplastic Agents; C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D004265:DNA Helicases; C471926:CHD5 protein, human; D005472:Fluorouracil
keywords: CHD5; HNSCC; cell proliferation; chemoresistance; miR-24-3p