title: Production and characterization of the exopolysaccharide produced by Paenibacillus jamilae grown on olive mill-waste waters.
abstract: Paenibacillus jamilae, a strain isolated from compost prepared with olive-mill wastewaters, produced an extracellular polysaccharide (EPS) when it was grown in a culture containing olive-mill waste waters (OMWW) as sole carbon and energy sources. Maximal EPS production in 100 mL batch-culture experiments (5.1 g L(-1)) was reached with a concentration of 80% of OMWW as fermentation substrate (v/v). Although an inhibitory effect was observed on growth and EPS production when OMWW concentration was increased, an appreciable amount of EPS (2.7 g L(-1)) was produced with undiluted OMWW. Sepharose CL-2B chromatography showed that the EPS presented two fractions, EPS I (>2000 kDa) and EPS II (500 kDa). Both fractions were characterized by GC-MS as two different acidic heteropolysaccharides containing glucose, galactose and mannose as the major components. The performed study made evident the possibility of using OMWW as substrate for the production of EPS by P. jamilae with a satisfactory yield.
authors: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, Granada, 18071, Spain.
authors: José Antonio
authors: JA
authors: Morillo
authors: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, Granada, 18071, Spain.
authors: Victor
authors: V
authors: Guerra Del Águila
authors: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, Granada, 18071, Spain.
authors: Margarita
authors: M
authors: Aguilera
authors: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, Granada, 18071, Spain.
authors: Alberto
authors: A
authors: Ramos-Cormenzana
authors: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, Granada, 18071, Spain. mmonteol@ugr.es.
authors: Mercedes
authors: M
authors: Monteoliva-Sánchez
keywords: Exopolysaccharide production; Olive-mill waste waters (OMWW); Paenibacillus jamilae