title: Poly(colloid)s: "Polymerization" of Poly(l-tyrosine)-silica Composite Particles through the Photoinduced Cross-Linking of Unmodified Proteins Method.
abstract: Photoinduced cross-linking of unmodified proteins, PICUP, was extended to core-shell silica-polypeptide composite particles to produce poly(colloid)s. Silica particles coated with poly(l-tyrosine), PTYR-SiO2, served as the monomer units. The PICUP reaction accomplished the formation of dityrosil linkages between the tyrosine units by illumination of photo-oxidizing ruthenium(II) bipyridyl catalyst under physiological conditions. The PICUP method was compared with an enzymatic route intermediated by horseradish peroxidase as catalyst. The PTYR-SiO2 particles feature high PTYR content in the shell, which facilitated the formation of heavily cross-linked but unstructured aggregates. After magnetic alignment of superparamagnetic PTYR-SiO2-cobalt composite particles, only the PICUP approach enabled the preparation of isolated chain-like poly(colloid)s. The cross-linking products were confirmed by FTIR. The native secondary structure of poly(l-tyrosine) is preserved in these poly(colloid)s. Because the PICUP reaction does not require the modification of the polypeptide structure, the cross-linked PTYR will retain its characteristic functions as a poly(amino acid). The PICUP method opens the door to a variety of PTYR-based poly(colloid) architectures.
authors: School of Materials Science and Engineering, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.|Department of Chemistry and Macromolecular Studies Group, Louisiana State University , Baton Rouge, Louisiana 70803, United States.|Georgia Tech Polymer Network, GTPN, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
authors: Cornelia
authors: C
authors: Rosu
authors: Department of Chemistry and Macromolecular Studies Group, Louisiana State University , Baton Rouge, Louisiana 70803, United States.
authors: Rafael
authors: R
authors: Cueto
authors: School of Materials Science and Engineering, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.|Department of Chemistry and Macromolecular Studies Group, Louisiana State University , Baton Rouge, Louisiana 70803, United States.|School of Chemistry and Biochemistry, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.|Georgia Tech Polymer Network, GTPN, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
authors: Paul S
authors: PS
authors: Russo
mesh_terms: D003035:Cobalt / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D003432:Cross-Linking Reagents; D000067493:Dynamic Light Scattering; D006735:Horseradish Peroxidase; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D055668:Photochemical Processes; D058105:Polymerization; D066329:Protein Aggregates; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003102:Colloids; D003432:Cross-Linking Reagents; D058185:Magnetite Nanoparticles; D010455:Peptides; D066329:Protein Aggregates; C017663:polytyrosine; D003035:Cobalt; D012822:Silicon Dioxide; D006735:Horseradish Peroxidase