title: Cellobiose dehydrogenase and chitosan-based lysozyme responsive materials for antimicrobial wound treatment.
abstract: The treatment of wound infection still constitutes a major threat in health care due to the increasing number of bacterial resistances and the difficulty of timely infection detection. Here, we present a smart antimicrobial system that is activated in case of infection based on elevated lysozyme activities. N-acetyl chitosan (degree of N-acetylation: 40%) was synthesized and hydrolysis by lysozyme in artificial wound fluid (AWF) was demonstrated. This resulted in the formation of N-acetylated chito oligosaccharides (COS) with a degree of polymerization of 2-5 units. The COS were shown to serve as substrate for cellobiose dehydrogenase (CDH) leading to the production of 1 mM antimicrobial hydrogen peroxide (H2O2) after 24 h incubation at 37°C in AWF. Growth inhibition was seen upon incubation of Escherichia coli and Staphylococcus aureus with this chitosan-CDH system over 8 h. This approach represents the first self-regulating system for the infection responsive inhibition of bacterial growth in response to lysozyme as infection biomarker. Biotechnol. Bioeng. 2017;114: 416-422. © 2016 Wiley Periodicals, Inc.
authors: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Straße 20, Tulln an der Donau, 3430, Austria.
authors: Christoph
authors: C
authors: Öhlknecht
authors: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Straße 20, Tulln an der Donau, 3430, Austria.
authors: Gregor
authors: G
authors: Tegl
authors: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Straße 20, Tulln an der Donau, 3430, Austria.
authors: Bianca
authors: B
authors: Beer
authors: Department of Food Sciences and Technology, University of Natural Resources and Life Sciences, Vienna, Austria.
authors: Christoph
authors: C
authors: Sygmund
authors: Department of Food Sciences and Technology, University of Natural Resources and Life Sciences, Vienna, Austria.
authors: Roland
authors: R
authors: Ludwig
authors: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Straße 20, Tulln an der Donau, 3430, Austria.|ACIB-Austrian Centre of Industrial Biotechnology, Tulln, Austria.
authors: Georg M
authors: GM
authors: Guebitz
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008954:Models, Biological; D009113:Muramidase / Q000737:chemistry / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D014946:Wound Infection / Q000382:microbiology / Q000517:prevention & control
chemical_list: D000890:Anti-Infective Agents; C012693:N-acetylchitosan; D048271:Chitosan; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D009113:Muramidase
keywords: N-acetyl chitosan; diagnostics; enzyme responsive; infection detection; infection prevention