title: Laboratory Cultivation and Maintenance of Borrelia miyamotoi.
abstract: Borrelia miyamotoi is a relapsing fever tick-borne pathogen found in Ixodes spp. (hard) ticks. In vitro culturing has proven difficult despite initial reports of cultures maintained in Barbour-Stoenner-Kelly-II (BSK-II) medium. The ability to culture in vitro opens many avenues for investigating the genetics and physiology of bacterial species. This unit describes methods for the maintenance and cultivation of B. miyamotoi in liquid medium. © 2016 by John Wiley & Sons, Inc.
authors: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, North Dakota.
authors: Brandee L
authors: BL
authors: Stone
authors: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, North Dakota.
authors: Catherine A
authors: CA
authors: Brissette
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001898:Borrelia / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003470:Culture Media / Q000378:metabolism; D006801:Humans; D012061:Relapsing Fever / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media
keywords: Borrelia miyamotoi; Ixodes; in vitro cultivation; pathogen; spirochete