title: Enhanced starch hydrolysis using α-amylase immobilized on cellulose ultrafiltration affinity membrane.
abstract: In order to prepare ultrafiltration membranes possessing biocatalytic properties, α-amylase has been immobilized on cellulose membranes. Enzyme immobilization was based on a covalent bonding between chitosan and a surface of cellulose membrane, followed by an attachment of Cibacron Blue F3G-A dye as affinity ligand. Various factors affecting the immobilization process, such as enzyme concentration, pH of modifying solution, zeta-potential of membrane surface, and stability of immobilized enzyme were studied. The applicability of immobilized α-amylase has been investigated in ultrafiltration processes. The immobilization of α-amylase on membrane surface allows to increase the value of mass transfer coefficient and to decrease the concentration polarization effect during ultrafiltration of starch solutions. The enzyme layer on the membrane surface prevents a rapid increase of starch concentration due to the amylase hydrolysis of starch in the boundary layer. The presented affinity immobilization technique allows also for the regeneration of membranes from inactivated enzyme.
authors: Department of Chemistry, National University of Kyiv-Mohyla Academy, 2 Skovoroda Street, 04070 Kiev, Ukraine.
authors: Viktoriia
authors: V
authors: Konovalova
authors: Department of Chemistry, National University of Kyiv-Mohyla Academy, 2 Skovoroda Street, 04070 Kiev, Ukraine.
authors: Kateryna
authors: K
authors: Guzikevich
authors: Department of Chemistry, National University of Kyiv-Mohyla Academy, 2 Skovoroda Street, 04070 Kiev, Ukraine.
authors: Anatoliy
authors: A
authors: Burban
authors: Nicolaus Copernicus University in Toruń, Faculty of Chemistry, 7 Gagarina Street, 87-100 Toruń, Poland. Electronic address: kujawski@chem.umk.pl.
authors: Wojciech
authors: W
authors: Kujawski
authors: Nicolaus Copernicus University in Toruń, 11 Gagarina Street, 87-100 Toruń, Poland.
authors: Karolina
authors: K
authors: Jarzynka
authors: Nicolaus Copernicus University in Toruń, Faculty of Chemistry, 7 Gagarina Street, 87-100 Toruń, Poland.
authors: Joanna
authors: J
authors: Kujawa
mesh_terms: D000069976:Bacillus licheniformis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006868:Hydrolysis; D008567:Membranes, Artificial; D013213:Starch / Q000737:chemistry*; D014462:Ultrafiltration; D000516:alpha-Amylases / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D002482:Cellulose; D013213:Starch; D000516:alpha-Amylases
keywords: Cellulose affinity membranes; Concentration polarization; Membrane surface modification; Starch hydrolysis; Ultrafiltration; α-Amylase immobilization