title: A pivotal role of vacuolar H(+)-ATPase in regulation of lipid production in Phaeodactylum tricornutum.
abstract: Microalgal lipids have been considered as a promising source for biodiesel production. Alkaline pH can induce neutral lipid accumulation in microalgae cells. However, whether and how proton pumps, especially vacuolar H(+)-ATPase (V-ATPase), function in these processes is not well known. In this study, we treated Phaeodactylum tricornutum with V-ATPase specific inhibitor bafilomycin A1 (BFA1) to determine its role in lipid production. Firstly, V-ATPase activity was increased in the latter phase of microalgae growth. BFA1 treatment decreased the cell density and lipid contents. Further analysis showed that BFA1 treatment reduced the number and size of oil bodies. GC-MS analysis showed that lipid components were not affected by BFA1 treatment. Intracellular pH was decreased and nitrogen depletion was delayed after BFA1 treatment. RNA-Seq analysis showed that expression of genes involved in calcium signaling, sulfur metabolism, cell cycle, glycolysis, pentose phosphate pathway, porphyrin, chlorophyll metabolism and lipid catabolic metabolism were upregulated, while expression of genes involved in ion transmembrane transport, ubiquitin mediated proteolysis, SNARE interactions in vesicular transport, fatty acid biosynthesis were downregulated under BFA1 treatment. Our findings provided insights into the molecular mechanisms underlying lipid accumulation and the key genes involved in lipid metabolism in Phaeodactylum tricornutum in response to BFA1.
authors: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|Center for Molecular Cell and Systems biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|Division of Ocean Science and Technology, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors: Huiying
authors: H
authors: Zhang
authors: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Rensen
authors: R
authors: Zeng
authors: Division of Ocean Science and Technology, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors: Daoyi
authors: D
authors: Chen
authors: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Jian
authors: J
authors: Liu
mesh_terms: D056804:Biofuels; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D002708:Chlorella / Q000378:metabolism; D017377:Diatoms / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007477:Ions; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000201:enzymology*; D009584:Nitrogen / Q000378:metabolism; D010427:Pentose Phosphate Pathway; D017423:Sequence Analysis, RNA; D025262:Vacuolar Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D007477:Ions; D008055:Lipids; D025262:Vacuolar Proton-Translocating ATPases; D009584:Nitrogen; D002118:Calcium