title: Protective efficacy of multiple vaccine platforms against Zika virus challenge in rhesus monkeys.
abstract: Zika virus (ZIKV) is responsible for a major ongoing epidemic in the Americas and has been causally associated with fetal microcephaly. The development of a safe and effective ZIKV vaccine is therefore an urgent global health priority. Here we demonstrate that three different vaccine platforms protect against ZIKV challenge in rhesus monkeys. A purified inactivated virus vaccine induced ZIKV-specific neutralizing antibodies and completely protected monkeys against ZIKV strains from both Brazil and Puerto Rico. Purified immunoglobulin from vaccinated monkeys also conferred passive protection in adoptive transfer studies. A plasmid DNA vaccine and a single-shot recombinant rhesus adenovirus serotype 52 vector vaccine, both expressing ZIKV premembrane and envelope, also elicited neutralizing antibodies and completely protected monkeys against ZIKV challenge. These data support the rapid clinical development of ZIKV vaccines for humans.
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Peter
authors: P
authors: Abbink
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Rafael A
authors: RA
authors: Larocca
authors: Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
authors: Rafael A
authors: RA
authors: De La Barrera
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Christine A
authors: CA
authors: Bricault
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Edward T
authors: ET
authors: Moseley
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Michael
authors: M
authors: Boyd
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Marinela
authors: M
authors: Kirilova
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Zhenfeng
authors: Z
authors: Li
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: David
authors: D
authors: Ng'ang'a
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Ovini
authors: O
authors: Nanayakkara
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Ramya
authors: R
authors: Nityanandam
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Noe B
authors: NB
authors: Mercado
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Erica N
authors: EN
authors: Borducchi
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Arshi
authors: A
authors: Agarwal
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Amanda L
authors: AL
authors: Brinkman
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Crystal
authors: C
authors: Cabral
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Abishek
authors: A
authors: Chandrashekar
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Patricia B
authors: PB
authors: Giglio
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: David
authors: D
authors: Jetton
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Jessica
authors: J
authors: Jimenez
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Benjamin C
authors: BC
authors: Lee
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Shanell
authors: S
authors: Mojta
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Katherine
authors: K
authors: Molloy
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Mayuri
authors: M
authors: Shetty
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: George H
authors: GH
authors: Neubauer
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors: Kathryn E
authors: KE
authors: Stephenson
authors: University of São Paulo, São Paulo 05508-000, Brazil.
authors: Jean Pierre S
authors: JP
authors: Peron
authors: University of São Paulo, São Paulo 05508-000, Brazil.
authors: Paolo M de A
authors: PM
authors: Zanotto
authors: Bioqual, Rockville, MD 20852, USA.
authors: Johnathan
authors: J
authors: Misamore
authors: Bioqual, Rockville, MD 20852, USA.
authors: Brad
authors: B
authors: Finneyfrock
authors: Bioqual, Rockville, MD 20852, USA.
authors: Mark G
authors: MG
authors: Lewis
authors: Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
authors: Galit
authors: G
authors: Alter
authors: Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA. Henry M. Jackson Foundation, Bethesda, MD 20817, USA.
authors: Kayvon
authors: K
authors: Modjarrad
authors: Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
authors: Richard G
authors: RG
authors: Jarman
authors: Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
authors: Kenneth H
authors: KH
authors: Eckels
authors: Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
authors: Nelson L
authors: NL
authors: Michael
authors: Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
authors: Stephen J
authors: SJ
authors: Thomas
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA. Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA. dbarouch@bidmc.harvard.edu.
authors: Dan H
authors: DH
authors: Barouch
mesh_terms: D000256:Adenoviridae; D019264:Adoptive Transfer; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D001938:Brazil; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D000071497:Immunogenicity, Vaccine; D007136:Immunoglobulins / Q000276:immunology / Q000302:isolation & purification; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011647:Puerto Rico; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000071244:Zika Virus / Q000276:immunology*; D000071243:Zika Virus Infection / Q000517:prevention & control*
chemical_list: D000914:Antibodies, Viral; D007136:Immunoglobulins; D019444:Vaccines, DNA; D015164:Vaccines, Inactivated; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C074932:prM protein, Flavivirus