title: Impedimetric immunosensor for detection of cardiovascular disorder risk biomarker.
abstract: We report the construction and characterization of a novel, level free impedimetric immunosensor for rapid, sensitive and selective detection of myoglobin (Mb). Monoclonal anti-myoglobin (anti-Mb-IgG) antibody was immobilized on screen-printed multiwalled carbon nanotubes electrode for signal amplification without the need of natural enzymes. The fabrication of resulting immunosensor was extensively characterized by using scanning electron microscopy (SEM), fourier transform infrared (FT-IR) spectroscopy, cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Electrochemical impedance spectroscopy (EIS) technique offered a linear detection range (0.1-90ngmL(-1)) of myoglobin with sensitivity of 0.74kΩngmL(-1) (correlation coefficient, R(2)=0.97) and detection limit of 0.08ngmL(-1) (S/N=3). The mean percentage recovery of Mb in serum samples using this working biosensor is 97.33%. Furthermore, the proposed strategy can be a promising alternative for detection of Mb related cardiovascular disorders.
authors: Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Academy of Scientific and Innovative Research, Jorhat 785006, Assam, India. Electronic address: khan.raju@gmail.com.
authors: Raju
authors: R
authors: Khan
authors: Biotechnology Division, CSIR-North East Institute of Science & Technology, Academy of Scientific and Innovative Research, Jorhat 785006, Assam, India.
authors: Mintu
authors: M
authors: Pal
authors: Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow 119121, Russia.
authors: Alexey V
authors: AV
authors: Kuzikov
authors: Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow 119121, Russia.
authors: Tanya
authors: T
authors: Bulko
authors: Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow 119121, Russia.
authors: Elena V
authors: EV
authors: Suprun
authors: Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow 119121, Russia.
authors: Victoria V
authors: VV
authors: Shumyantseva
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods*; D002318:Cardiovascular Diseases / Q000097:blood*; D058266:Dielectric Spectroscopy / Q000379:methods*; D006736:Horses; D007074:Immunoglobulin G / Q000737:chemistry*; D009211:Myoglobin / Q000097:blood*
chemical_list: D015415:Biomarkers; D007074:Immunoglobulin G; D009211:Myoglobin
keywords: Cardiovascular disease; Electrochemical impedance spectroscopy; Immunosensors; Myoglobin; Screen-printed multiwalled carbon nanotubes electrode