title: Oligosaccharides from the 3-linked 2-sulfated alpha-L-fucan and alpha-L-galactan show similar conformations but different dynamics.
abstract: Here we have performed an nuclear magnetic resonance-based study on the ring and chain conformations as well as dynamics of oligosaccharides generated by acid hydrolysis on two structurally related glycans, a 3-linked 2-sulfated alpha-L-galactan and a 3-linked 2-sulfated alpha-L-fucan. Results derived from scalar couplings have confirmed the1C4chair configuration to both alpha-L-fucose and alpha-L-galactose, and a similar solution 3D structure for the oligosaccharide chains of both sulfated glycans as seen on the basis of NOE patterns. Measurements of spin-relaxation rates have suggested, however, a slight difference dynamical property to these glycans. The fucose-based oligosaccharides showed an enhanced dynamical property if compared to the galactose-based oligosaccharides of same anomericity, sugar configuration, glycosidic bond and sulfation type. This distinction solely on the dynamical aspect has been driven therefore by the different sugar composition of the two studied sulfated glycans.
authors: Program of Glycobiology, Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil.|University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21941-913, Brazil.
authors: Ismael N L
authors: IN
authors: Queiroz
authors: University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21941-913, Brazil.|Institute of Biomedical Sciences, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil.
authors: Ana-Cristina E S
authors: AE
authors: Vilela-Silva
authors: Program of Glycobiology, Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil pominvh@bioqmed.ufrj.br vhpomin@gmail.com.|University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21941-913, Brazil.
authors: Vitor H
authors: VH
authors: Pomin
mesh_terms: D002236:Carbohydrate Conformation; D005685:Galactans / Q000737:chemistry*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy / Q000592:standards; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry*; D011789:Quantum Theory; D012015:Reference Standards
chemical_list: D005685:Galactans; D011134:Polysaccharides
keywords: NMR; conformation; dynamics; sulfated fucan; sulfated galactan