title: Genome-wide identification and characterization of the NF-Y gene family in grape (vitis vinifera L.).
abstract: Nuclear factor Y (NF-Y) transcription factor is composed of three distinct subunits: NF-YA, NF-YB and NF-YC. Many members of NF-Y family have been reported to be key regulators in plant development, phytohormone signaling and drought tolerance. However, the function of the NF-Y family is less known in grape (Vitis vinifera L.).
  
  
  
  A total of 34 grape NF-Y genes that distributed unevenly on grape (V. vinifera) chromosomes were identified in this study. Phylogenetic analysis was performed to predict functional similarities between Arabidopsis thaliana and grape NF-Y genes. Comparison of the structures of grape NF-Y genes (VvNF-Ys) revealed their functional conservation and alteration. Furthermore, we investigated the expression profiles of VvNF-Ys in response to various stresses, phytohormone treatments, and in leaves and grape berries with various sugar contents at different developmental stages. The relationship between VvNF-Y transcript levels and sugar content was examined to select candidates for exogenous sugar treatments. Quantitative real-time PCR (qPCR) indicated that many VvNF-Ys responded to different sugar stimuli with variations in transcript abundance. qPCR and publicly available microarray data suggest that VvNF-Ys exhibit distinct expression patterns in different grape organs and developmental stages, and a number of VvNF-Ys may participate in responses to multiple abiotic and biotic stresses, phytohormone treatments and sugar accumulation or metabolism.
  
  
  
  In this study, we characterized 34 VvNF-Ys based on their distributions on chromosomes, gene structures, phylogenetic relationship with Arabidopsis NF-Y genes, and their expression patterns. The potential roles of VvNF-Ys in sugar accumulation or metabolism were also investigated. Altogether, the data provide significant insights on VvNF-Ys, and lay foundations for further functional studies of NF-Y genes in grape.
authors: Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resource, Institute of Botany, the Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors: Chong
authors: C
authors: Ren
authors: Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resource, Institute of Botany, the Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors: Zhan
authors: Z
authors: Zhang
authors: Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resource, Institute of Botany, the Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors: Yi
authors: Y
authors: Wang
authors: Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resource, Institute of Botany, the Chinese Academy of Sciences, Beijing, 100093, People's Republic of China. shhli@ibcas.ac.cn.
authors: Shaohua
authors: S
authors: Li
authors: Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resource, Institute of Botany, the Chinese Academy of Sciences, Beijing, 100093, People's Republic of China. ZL249@ibcas.ac.cn.
authors: Zhenchang
authors: Z
authors: Liang
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D023081:CCAAT-Binding Factor / Q000235:genetics* / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000737:chemistry; D005638:Fruit / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D005810:Multigene Family; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013312:Stress, Physiological; D059467:Transcriptome; D027843:Vitis / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D023081:CCAAT-Binding Factor; D010940:Plant Proteins; D021122:Protein Subunits; C402244:nuclear factor Y
keywords: Expression profiles; Grape (Vitis vinifera L.); NF-Y transcription factor; Phylogenetic analysis; Quantitative real-time PCR