title: Establishment and characterization of a cell line derived from Eptesicus nilssonii.
abstract: Bats of the genus Eptesicus have several non-retroviral RNA virus-derived sequences in their genomes, among which an endogenous bornavirus-like L element, named eEBLL-1, was suggested to encode functional proteins in the hosts. However, the function of eEBLL-1 remains unclear due to a lack of appropriate investigation tools, such as cultured cells expressing eEBLL-1. Here, we established a continuous cell line, named HAMOI-EnK cells, from kidney of Eptesicus nilssonii. HAMOI-EnK cells are robust and could be passaged for at least 10 months. eEBLL-1 in the genomes of HAMOI-EnK cells retains an intact open reading frame. Additionally, eEBLL-1 is transcribed in the sense-orientation in cells. To our knowledge, this is the first report to demonstrate that eEBLL-1 is transcribed in cultured cells.
authors: Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
authors: Masayuki
authors: M
authors: Horie
authors: Takumi
authors: T
authors: Akasaka
authors: Sachiko
authors: S
authors: Matsuda
authors: Haruko
authors: H
authors: Ogawa
authors: Kunitoshi
authors: K
authors: Imai
mesh_terms: D000818:Animals; D028981:Bornaviridae / Q000235:genetics*; D002460:Cell Line / Q000378:metabolism*; D002685:Chiroptera / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D007668:Kidney / Q000166:cytology; D016366:Open Reading Frames