title: Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii in South America.
abstract: One hundred and twenty-six epidemiologically sequential, unrelated, carbapenem-resistant Acinetobacter baumannii isolates from nine hospitals in six countries of South America were collected between July 2013 and June 2014. Genes coding for Ambler class D and B carbapenemases were sought by PCR. All isolates were typed using the 3-locus sequence typing and blaOXA-51-like sequence-based typing techniques. The blaOXA-23 gene was recovered in all the participating hospitals and in all the isolates of seven of nine medical centres. The blaOXA-72 gene was only recovered in the two medical centres from Guayaquil city, Ecuador. Trilocus sequence typing revealed the presence of sequence groups SG2, SG4 and SG5. blaOXA-51-like sequence-based typing revealed the presence of blaOXA-132, blaOXA-65, blaOXA-69 and blaOXA-64. Our results showed that the population of carbapenem-resistant A. baumannii in South America was principally associated with ST79, ST25 and ST15 (92 %) and harboured the blaOXA-23 gene mainly. CC2 was not detected.
authors: Laboratorio de Bacteriología, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors: Carlos Hernán
authors: CH
authors: Rodríguez
authors: Laboratorio de Bacteriología, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors: Norah
authors: N
authors: Balderrama Yarhui
authors: Laboratorio de Bacteriología, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors: Marcela
authors: M
authors: Nastro
authors: Hospital IESS 'Teodoro Maldonando Carbo', Guayaquil, Ecuador.
authors: Tamara
authors: T
authors: Nuñez Quezada
authors: Hospital de Infectología 'José Daniel Rodríguez Maridueña', Guayaquil, Ecuador.
authors: Glenda
authors: G
authors: Castro Cañarte
authors: Hospital Materno Iinfantil 'San Martín de Porres', Ibuelo, Cochabamba, Bolivia.
authors: Raquel
authors: R
authors: Magne Ventura
authors: Hospital del 'Niño Manuel Ascencio Villarroel', Cochabamba, Bolivia.
authors: Tayita
authors: T
authors: Ugarte Cuba
authors: Hospital 'Dr Leonardo Guzmán', Antofagasta, Chile.
authors: Natalia
authors: N
authors: Valenzuela
authors: Hospital 'Dr Leonardo Guzmán', Antofagasta, Chile.
authors: Freddy
authors: F
authors: Roach
authors: Cooperativa Asistencial Médica del Este de Colonia, Colonia, Rosario, Uruguay.
authors: María Inés
authors: MI
authors: Mota
authors: Cooperativa Asistencial Médica del Este de Colonia, Colonia, Rosario, Uruguay.
authors: Noelia
authors: N
authors: Burger
authors: Hospital de Clínicas FCM, UMA, Asunción, Paraguay.
authors: Gladys
authors: G
authors: Velázquez Aguayo
authors: Hospital de Clínicas FCM, UMA, Asunción, Paraguay.
authors: Juana
authors: J
authors: Ortellado-Canese
authors: Hospital Nuestro Señora Del Carmen (OSEP), Mendoza, Argentina.
authors: Geni
authors: G
authors: Bruni
authors: Hospital Nuestro Señora Del Carmen (OSEP), Mendoza, Argentina.
authors: Cecilia
authors: C
authors: Pandolfo
authors: Hospital Nuestro Señora Del Carmen (OSEP), Mendoza, Argentina.
authors: Nadya
authors: N
authors: Bastyas
authors: Laboratorio de Bacteriología, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors: Angela
authors: A
authors: Famiglietti
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D015780:Carbapenems / Q000494:pharmacology*; D005838:Genotype; D006761:Hospitals; D006801:Humans; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D013020:South America / Q000453:epidemiology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase