title: Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease.
abstract: The aim of this study is to investigate the correlation between serum microRNA-217 and the severity of diabetic kidney disease determined by albuminuria. Four hundred ninety five type 2 diabetes patients were divided into three groups: normoalbuminuric group, microalbuminuric group, and macroalbuminuric group. Serum microRNA-217 levels were validated by real-time polymerase chain reaction. Serum silent information regulator 1, Hypoxia-inducible factor-1α and vascular endothelial growth factor were determined by enzyme-linked immunosorbent assay. Compared with control, serum microRNA-217 levels were significantly increased in type 2 diabetes patients and gradually increased in patients of normoalbuminuric, microalbuminuric, and macroalbuminuric groups (P < 0.01). Moreover, increased levels of serum microRNA-217, hypoxia-inducible factor-1α, vascular endothelial growth factor, diabetes mellitus duration, fasting blood glucose, fasting insulin, homeostasis model assessment for insulin resistance, glycated hemoglobin, low-density lipoprotein, total cholesterol, triglyceride, uric acid, serum creatinine, blood urea nitrogen, and decreased levels of serum silent information regulator 1 and high-density lipoprotein were significantly correlated with Ln(ACR) (P < 0.05). In addition, serum microRNA-217 was positively correlated with diabetes mellitus duration, homeostasis model assessment for insulin resistance, glycated hemoglobin, Ln(ACR), low-density lipoprotein, total cholesterol, triglyceride, uric acid, serum creatinine, blood urea nitrogen, hypoxia-inducible factor-1α, vascular endothelial growth factor (P < 0.05), and negatively correlated with serum silent information regulator 1 (P = 0.002). Our findings suggest that microRNA-217 may have an association with the development of proteinuria in type 2 diabetes patients. Serum microRNA-217 may be involved in the development of diabetic kidney disease by promoting chronic inflammation, renal fibrosis, and angiogenesis.
authors: Department of Endocrinology, The First Hospital Affiliated to China Medical University, Shenyang, Liaoning, China.
authors: Ying
authors: Y
authors: Shao
authors: Department of Endocrinology, The First Hospital Affiliated to China Medical University, Shenyang, Liaoning, China.
authors: Huiwen
authors: H
authors: Ren
authors: Department of Endocrinology, People's Hospital of China Medical University, Shenyang, Liaoning, China.
authors: Chuan
authors: C
authors: Lv
authors: Cadre Department, The First Hospital Affiliated to China Medical University, Shenyang, Liaoning, China.
authors: Xiaoyu
authors: X
authors: Ma
authors: Department of Endocrinology, The First Hospital Affiliated to China Medical University, Shenyang, Liaoning, China.
authors: Can
authors: C
authors: Wu
authors: Department of Endocrinology, The First Hospital Affiliated to China Medical University, Shenyang, Liaoning, China. wqycmu123@163.com.
authors: Qiuyue
authors: Q
authors: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000419:Albuminuria / Q000209:etiology; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002681:China; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications* / Q000378:metabolism / Q000628:therapy; D003928:Diabetic Nephropathies / Q000097:blood / Q000503:physiopathology* / Q000652:urine; D018450:Disease Progression; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000097:blood; D007333:Insulin Resistance; D007668:Kidney / Q000503:physiopathology*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D051437:Renal Insufficiency / Q000097:blood / Q000150:complications* / Q000503:physiopathology / Q000652:urine; D012720:Severity of Illness Index; D056564:Sirtuin 1 / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: D015415:Biomarkers; D006442:Glycated Hemoglobin; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D035683:MicroRNAs; D056564:Sirtuin 1; D042461:Vascular Endothelial Growth Factor A; C497442:HIF1A protein, human; C553315:MIRN217 microRNA, human; C467484:VEGFA protein, human; C517652:hemoglobin A1c protein, human; C447939:SIRT1 protein, human
keywords: Albuminuria; Diabetic kidney disease; Hypoxia-inducible factor-1α; Serum microRNA-217; Silent information regulator 1