title: Zeolite scaffolds for cultures of human breast cancer cells. Part II: Effect of pure and hybrid zeolite membranes on neoplastic and metastatic activity control.
abstract: This work is focused on the response of two invasive phenotypes of human breast cancer cells, MCF-7 and MDA-MB-231, grown on synthesized zeolite scaffolds in order to study the influence of those biomaterials in controlled conditions with and without anti-tumoral drug treatments. Our research was directed to the use of doxorubicin (DOX) and bergapten (5-MOP). The former is broadly considered the most active single agent available for the treatment of breast cancer, the second is a natural psoralen with an apoptotic effect. The results indicate that both drugs inhibit the cell viability of all cell lines grown on all zeolite scaffolds and that all Pure Zeolite Membranes are more responsive with respect to all Mixed Matrix Membranes. Moreover, the results after treatment with DOX at a concentration of 7.4μM for 24h, show that the expression of the matrix metalloproteinases (MMP-2 and MMP-9) is greatly reduced in both cell lines, especially in those adherent on Pure Zeolite Scaffolds.
authors: Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Cubo 4/c, 87036, Rende, Italy. Electronic address: p.tavolaro@unical.it.
authors: Palmira
authors: P
authors: Tavolaro
authors: Department Di.B.E.S.T. (Biologia, Ecologia, Scienze della Terra), Unit of Physiology, University of Calabria, Cubo 4/c, 87036 Rende, Italy.
authors: Guglielmo
authors: G
authors: Martino
authors: Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Cubo 4/c, 87036, Rende, Italy.
authors: Sebastiano
authors: S
authors: Andò
authors: Research Institute on Membrane Technology, Unit of Zeolite Membranes, ITM-CNR, University of Calabria, Cubo 17/c, 87036 Rende, Italy.
authors: Adalgisa
authors: A
authors: Tavolaro
mesh_terms: D000078223:5-Methoxypsoralen; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D018929:Cell Culture Techniques; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D008567:Membranes, Artificial; D008730:Methoxsalen / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D009362:Neoplasm Metastasis; D054457:Tissue Scaffolds / Q000737:chemistry*; D017641:Zeolites / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D017641:Zeolites; D000078223:5-Methoxypsoralen; D004317:Doxorubicin; D008730:Methoxsalen
keywords: Bergapten; Doxorubicin; MCF-7 breast cancer cells; MDA-MB-231 breast cancer cells; Matrix metalloproteinases; Zeolite scaffolds