title: Engineered porous scaffolds for periprosthetic infection prevention.
abstract: Periprosthetic infection is a consequence of implant insertion procedures and strategies for its prevention involve either an increase in the rate of new bone formation or the release of antibiotics such as vancomycin. In this work we combined both strategies and developed a novel, multifunctional three-dimensional porous scaffold that was produced using hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), coupled with a pectin (PEC)-chitosan (CHIT) polyelectrolyte (PEI), and loaded with vancomycin (VCA). By this approach, a controlled vancomycin release was achieved and serial bacterial dilution test demonstrated that, after 1week, the engineered construct still inhibits the bacterial growth. Degradation tests show an excellent behavior in a physiological and acidic environment (<10% of mass loss). Furthermore, the PEI coating shows an anti-inflammatory response, and good cell proliferation and migration were demonstrated in vitro using osteoblast SAOS-2 cell line. This new engineered construct exhibits excellent properties both as an antibacterial material and as a stimulator of bone formation, which makes it a good candidate to contrast periprosthetic infection.
authors: Nobil Bio Ricerche Srl, 14037 Portacomaro, Italy; Department of Applied Science and Technology, Institute of Materials Physics and Engineering, Politecnico di Torino, 10121 Torino, Italy. Electronic address: giorgio.iviglia@polito.it.
authors: Giorgio
authors: G
authors: Iviglia
authors: Nobil Bio Ricerche Srl, 14037 Portacomaro, Italy.
authors: Clara
authors: C
authors: Cassinelli
authors: Nobil Bio Ricerche Srl, 14037 Portacomaro, Italy.
authors: Daniele
authors: D
authors: Bollati
authors: Department of Applied Science and Technology, Institute of Materials Physics and Engineering, Politecnico di Torino, 10121 Torino, Italy.
authors: Francesco
authors: F
authors: Baino
authors: Nobil Bio Ricerche Srl, 14037 Portacomaro, Italy.
authors: Elisa
authors: E
authors: Torre
authors: Nobil Bio Ricerche Srl, 14037 Portacomaro, Italy.
authors: Marco
authors: M
authors: Morra
authors: Department of Applied Science and Technology, Institute of Materials Physics and Engineering, Politecnico di Torino, 10121 Torino, Italy.
authors: Chiara
authors: C
authors: Vitale-Brovarone
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry; D020007:Implants, Experimental / Q000382:microbiology*; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism / Q000382:microbiology*; D010368:Pectins / Q000737:chemistry; D016062:Porosity; D013203:Staphylococcal Infections / Q000517:prevention & control*; D013212:Staphylococcus epidermidis / Q000254:growth & development*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014640:Vancomycin / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D002130:Calcium Phosphates; D003692:Delayed-Action Preparations; C485817:beta-tricalcium phosphate; D014640:Vancomycin; D010368:Pectins; D048271:Chitosan; D017886:Durapatite
keywords: Antibacterial; Periprosthetic infection (PPI); Polyelectrolyte; Porous scaffold; Vancomycin