title: High-Density Protein Loading on Hierarchically Porous Layered Double Hydroxide Composites with a Rational Mesostructure.
abstract: Hierarchically porous biocompatible Mg-Al-Cl-type layered double hydroxide (LDH) composites containing aluminum hydroxide (Alhy) have been prepared using a phase-separation process. The sol-gel synthesis allows for the hierarchical pores of the LDH-Alhy composites to be tuned, leading to a high specific solid surface area per unit volume available for high-molecular-weight protein adsorptions. A linear relationship between the effective surface area, SEFF, and loading capacity of a model protein, bovine serum albumin (BSA), is established following successful control of the structure of the LDH-Alhy composite. The threshold of the mean pore diameter, Dpm, above which BSA is effectively adsorbed on the surface of LDH-Alhy composites, is deduced as 20 nm. In particular, LDH-Alhy composite aerogels obtained via supercritical drying exhibit an extremely high capacity for protein loading (996 mg/g) as a result of a large mean mesopore diameter (>30 nm). The protein loading on LDH-Alhy is >14 times that of a reference LDH material (70 mg/g) prepared via a standard procedure. Importantly, BSA molecules pre-adsorbed on porous composites were successfully released on soaking in ionic solutions (HPO4(2-) and Cl(-) aqueous). The superior capability of the biocompatible LDH materials for loading, encapsulation, and releasing large quantities of proteins was clearly demonstrated.
authors: Department of Materials Science, Graduate School of Engineering, Osaka Prefecture University , Sakai, Osaka 599-8531, Japan.
authors: Yasuaki
authors: Y
authors: Tokudome
authors: Department of Materials Science, Graduate School of Engineering, Osaka Prefecture University , Sakai, Osaka 599-8531, Japan.
authors: Megu
authors: M
authors: Fukui
authors: Department of Materials Science, Graduate School of Engineering, Osaka Prefecture University , Sakai, Osaka 599-8531, Japan.
authors: Naoki
authors: N
authors: Tarutani
authors: Department of Materials Science, Graduate School of Engineering, Osaka Prefecture University , Sakai, Osaka 599-8531, Japan.
authors: Sari
authors: S
authors: Nishimura
authors: Institut de Chimie de Clermont-Ferrand (ICCF), Université Clermont Auvergne, Université Blaise Pascal , BP 10448, F-63000 Clermont-Ferrand, France.|Institut de Chimie de Clermont-Ferrand (ICCF), UMR 6296, Centre National de la Recherche Scientifique (CNRS) , F-63171 Aubiere, France.
authors: Vanessa
authors: V
authors: Prevot
authors: Institut de Chimie de Clermont-Ferrand (ICCF), Université Clermont Auvergne, Université Blaise Pascal , BP 10448, F-63000 Clermont-Ferrand, France.|Institut de Chimie de Clermont-Ferrand (ICCF), UMR 6296, Centre National de la Recherche Scientifique (CNRS) , F-63171 Aubiere, France.
authors: Claude
authors: C
authors: Forano
authors: School of Materials, The University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Gowsihan
authors: G
authors: Poologasundarampillai
authors: School of Materials, The University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Peter D
authors: PD
authors: Lee
authors: Department of Materials Science, Graduate School of Engineering, Osaka Prefecture University , Sakai, Osaka 599-8531, Japan.
authors: Masahide
authors: M
authors: Takahashi