title: The contribution of adenines in the catalytic core of 10-23 DNAzyme improved by the 6-amino group modifications.
abstract: In the catalytic core of 10-23 DNAzyme, its five adenine residues are moderate conservative, but with highly conserved functional groups like 6-amino group and 7-nitrogen atom. It is this critical conservation that these two groups could be modified for better contribution. With 2'-deoxyadenosine analogues, several functional groups were introduced at the 6-amino group of the five adenine residues. 3-Aminopropyl substituent at 6-amino group of A15 resulted in a five-fold increase of kobs. More efficient DNAzymes are expected by delicate design of the linkage and the external functional groups for this 6-amino group of A15. With this modification approach, other functional groups or residues could be optimized for 10-23 DNAzyme.
authors: College of Life Science, Guizhou University, Guiyang 550025, China.
authors: Junfei
authors: J
authors: Zhu
authors: College of Life Science, Guizhou University, Guiyang 550025, China.
authors: Zhiwen
authors: Z
authors: Li
authors: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China; Hununbeier People's Hospital, Hununbeier 021008, China.
authors: Qi
authors: Q
authors: Wang
authors: Guangxi Medical University, Nanning 530021, China.
authors: Yang
authors: Y
authors: Liu
authors: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. Electronic address: hejunlin@bmi.ac.cn.
authors: Junlin
authors: J
authors: He
mesh_terms: D000225:Adenine / Q000737:chemistry*; D020134:Catalytic Domain; D021881:DNA, Catalytic / Q000737:chemistry*
chemical_list: D021881:DNA, Catalytic; D000225:Adenine
keywords: 10-23 DNAzyme; Adenine; Amino group; Chemical modification; Imidazolyl group