title: Investigation of metal ion interaction with a lipid cubic phase using electrochemical impedance spectroscopy.
abstract: OBJECTIVE
  Electrochemical impedance spectroscopy, EIS, can be used as a complementary technique to investigate ion interaction with the headgroup region in the aqueous channels of a lipid cubic phase, LCP.
  
  
  METHODS
  A freestanding membrane made of monoolein LCP was formed by filling a small aperture that separates two cell compartments. The cell compartments were filled with electrolyte solutions at two different ionic strengths: i.e.: 10 and 100mM, of KCl, CsBr and CaCl2. Electrochemical impedance spectroscopy was recorded between two platinum electrodes that were present at each side of the membrane.
  
  
  RESULTS
  The membrane resistance and capacitance were estimated from equivalent circuit fitting of the impedance data. It was confirmed that calcium ions interacts strongly with the headgroup region in the aqueous channels giving significantly higher membrane resistances compared to monovalent alkali metal ions. The membrane capacitance with Ca(2+)(aq) in solution was concentration dependent, which for the first time indicates formation of two different cubic phases at these conditions.
authors: Department of Chemistry, Umeå University, SE 90187 Umeå, Sweden.
authors: Mohammad Yaser Khani
authors: MYK
authors: Meynaq
authors: Department of Chemistry, Umeå University, SE 90187 Umeå, Sweden; Physical and Theoretical Chemistry Laboratory, Department of Chemistry, Oxford University, South Parks Road, Oxford OX1 3QZ, United Kingdom.
authors: Kenichi
authors: K
authors: Shimizu
authors: Department of Chemistry, Umeå University, SE 90187 Umeå, Sweden.
authors: Mahdi Shahmohammadi
authors: MS
authors: Aghbolagh
authors: Department of Chemistry, Umeå University, SE 90187 Umeå, Sweden.
authors: Solomon
authors: S
authors: Tesfalidet
authors: Department of Chemistry, Umeå University, SE 90187 Umeå, Sweden. Electronic address: britta.sethson@chem.umu.se.
authors: Britta
authors: B
authors: Lindholm-Sethson
keywords: Calcium; Cesium; Electrochemical impedance spectroscopy; Ionic permeability; Lipid cubic phase; Lipid–ion interactions; Membrane capacitance; Membrane resistance; Monoolein; Potassium