title: Structural transformation of synthetic hydroxyapatite under simulated in vivo conditions studied with ATR-FTIR spectroscopic imaging.
abstract: Hydroxyapatite and carbonate-substituted hydroxyapatite are widely used in bone tissue engineering and regenerative medicine. Both apatite materials were embedded into recently developed ceramic/polymer composites, subjected to Simulated Body Fluid (SBF) for 30days and characterized using ATR-FTIR spectroscopic imaging to assess their behaviour and structures. The specific aim was to detect the transition phases between both types of hydroxyapatite during the test and to analyze the surface modification caused by SBF. ATR-FTIR spectroscopic imaging was successfully applied to characterise changes in the hydroxyapatite lattice due to the elastic properties of the scaffolds. It was observed that SBF treatment caused a replacement of phosphates in the lattice of non-substituted hydroxyapatite by carbonate ions. A detailed study excluded the formation of pure A type carbonate apatite. In turn, CO32-content in synthetic carbonate-substituted hydroxyapatite decreased. The usefulness of ATR-FTIR spectroscopic imaging studies in the evaluation of elastic and porous β-glucan hydroxyapatite composites has been demonstrated.
authors: Department of Chemical Engineering, Imperial College London, South Kensington Campus, SW7 2AZ London, United Kingdom. Electronic address: annasroka@tlen.pl.
authors: Anna
authors: A
authors: Sroka-Bartnicka
authors: Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland.
authors: Leszek
authors: L
authors: Borkowski
authors: Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland.
authors: Grazyna
authors: G
authors: Ginalska
authors: Faculty of Materials Science and Ceramics, AGH-University of Science and Technology, Mickiewicza 30, 30-059 Krakow, Poland.
authors: Anna
authors: A
authors: Ślósarczyk
authors: Department of Chemical Engineering, Imperial College London, South Kensington Campus, SW7 2AZ London, United Kingdom. Electronic address: s.kazarian@imperial.ac.uk.
authors: Sergei G
authors: SG
authors: Kazarian
mesh_terms: D001826:Body Fluids / Q000737:chemistry*; D002254:Carbonates / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D021621:Imaging, Three-Dimensional; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002254:Carbonates; D017886:Durapatite
keywords: ATR FT-IR imaging; Biomaterials; Composites; Hydroxyapatite; Infrared spectroscopy; Structural transformation