title: E-beam crosslinked nanogels conjugated with monoclonal antibodies in targeting strategies.
abstract: Poly(N-vinyl pyrrolidone)-based-nanogels (NGs), produced by e-beam irradiation, are conjugated with monoclonal antibodies (mAb) for active targeting purposes. The uptake of immuno-functionalized nanogels is tested in an endothelial cell line, ECV304, using confocal and epifluorescence microscopy. Intracellular localization studies reveal a faster uptake of the immuno-nanogel conjugate with respect to the 'bare' nanogel. The specific internalization pathway of these immuno-nanogels is clarified by selective endocytosis inhibition experiments, flow cytometry and confocal microscopy. Active targeting ability is also verified by conjugating a monoclonal antibody which recognizes the αvβ3 integrin on activated endothelial cells. Epifluorescence images of the 'wound healing assay' on ECV304 cells provide evidence of nanogels localization only in the target cells. Therefore, the immuno-nanogels produced have the potential to recognize specific cell types in heterogeneous systems, which makes them promising candidates for targeted drug delivery applications.
authors: Giorgia
authors: G
authors: Adamo
authors: Natascia
authors: N
authors: Grimaldi
authors: Maria Antonietta
authors: MA
authors: Sabatino
authors: Marta
authors: M
authors: Walo
authors: Clelia
authors: C
authors: Dispenza
authors: Giulio
authors: G
authors: Ghersi
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D001692:Biological Transport; D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry*; D005782:Gels; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D011205:Povidone / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D005782:Gels; D011205:Povidone