title: Qualitative and Quantitative Study of the Potential of Lipid Nanocapsules of One Hundred Twenty Nanometers for the Topical Administration of Hydrophobic Molecules.
abstract: In this study, we evaluated the potential of lipid nanocapsules (LNC) of 120 nm as drug nanocarriers to treat skin diseases. As a model molecule, we encapsulated the fluorescent dye curcumin, which also is an antioxidant. Curcumin-loaded LNC showed interesting antioxidant properties and a low toxicity on human skin cells. The penetration of curcumin in the skin was determined by 2 complementary methods: high performance liquid chromatography was used to measure total curcumin accumulation in the skin, whereas fluorescence confocal spectral imaging of skin sections showed that curcumin preferentially accumulates in the stratum corneum and the viable epidermis. These results confirm that LNC of a size above 100 nm can vectorize hydrophobic compounds to the keratinocytes without transdermal delivery. They also demonstrate the interest of combining 2 analytical methods when studying the skin penetration of nanovectorized molecules.
authors: EA6295 Nanomédicaments et Nanosondes, Université François Rabelais de Tours, 31 avenue Monge, Tours 37200, France.
authors: Hoang Truc Phuong
authors: HTP
authors: Nguyen
authors: EA6295 Nanomédicaments et Nanosondes, Université François Rabelais de Tours, 31 avenue Monge, Tours 37200, France. Electronic address: emilie.munnier@univ-tours.fr.
authors: Emilie
authors: E
authors: Munnier
authors: EA6295 Nanomédicaments et Nanosondes, Université François Rabelais de Tours, 31 avenue Monge, Tours 37200, France.
authors: Xavier
authors: X
authors: Perse
authors: Spincontrol France, 298 rue Giraudeau, Tours 37000, France.
authors: Francis
authors: F
authors: Vial
authors: Bioeurope (Solabia Group), Route d'Oulins, Anet 28260, France.
authors: Florent
authors: F
authors: Yvergnaux
authors: Carlina Technologies, 22 rue Roger Amsler, Angers 49100, France.
authors: Thomas
authors: T
authors: Perrier
authors: EA6295 Nanomédicaments et Nanosondes, Université François Rabelais de Tours, 31 avenue Monge, Tours 37200, France.
authors: Martin
authors: M
authors: Soucé
authors: EA6295 Nanomédicaments et Nanosondes, Université François Rabelais de Tours, 31 avenue Monge, Tours 37200, France.
authors: Igor
authors: I
authors: Chourpa
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems / Q000379:methods; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects*; D008055:Lipids / Q000008:administration & dosage / Q000737:chemistry; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry; D009924:Organ Culture Techniques; D012869:Skin Absorption / Q000187:drug effects* / Q000502:physiology; D013552:Swine
chemical_list: D008055:Lipids; D053769:Nanocapsules; D003474:Curcumin
keywords: curcumin; drug delivery; fluorescence confocal spectral imaging; nanomedicine; skin