title: APOBEC3B deletion impacts on susceptibility to acquire HIV-1 and its advancement among individuals in western India.
abstract: APOBEC3B deletion polymorphism has been associated with risk of HIV-1 acquisition and its progression. Therefore, we aimed to investigate the association of APOBEC3B ins/del polymorphism with risk of acquisition of HIV-1 and its progression. In the present case-control study, we enrolled a total of 150 HIV-infected individuals and 150 healthy controls. Polymorphism for APOBEC3B gene was genotyped by PCR. APOBEC3B ID, DD genotypes, and D allele were associated with higher risk of acquisition of HIV-1 (p = 0.004, OR = 4.96; p = 0.03, OR = 3.55; and p = 0.004; OR = 1.60). The individuals with ID genotypes and combined genotype ID+DD of APOBEC3B in the presence of tobacco and alcohol showed the higher risk of advancement of HIV disease; however, risk could not reach statistical significance (OR = 1.14, 95% CI: 0.59-2.18; OR = 1.33, 95% CI: 0.83-2.15 and OR = 1.44, 95% CI: 0.77-2.69; OR = 1.50, 95% CI: 0.94-2.40). Individuals in advanced HIV disease stage and ID genotype and combined genotype ID + DD of APOBEC3B were more likely to be associated with advanced HIV disease stage but risk could not reach significant (OR = 1.50, 95% CI: 0.94-2.40; OR = 1.27, 95% CI: 0.88-1.84). Individuals with ID and DD genotype of APOBEC3B had influence on susceptibility to acquisition of HIV-1. This suggests that APOBEC3B deletion may attenuate innate cellular immunity against HIV-1 and thus confer the host persistence for HIV infection.
authors: Department of Molecular Biology, National AIDS Research Institute, Pune, India. hsingh@nariindia.org, hariomsgpgims@gmail.com.
authors: HariOm
authors: H
authors: Singh
authors: Department of Molecular Biology, National AIDS Research Institute, Pune, India.
authors: Shruti D
authors: SD
authors: Marathe
authors: Department of Molecular Biology, National AIDS Research Institute, Pune, India.
authors: Sumitra
authors: S
authors: Nain
authors: Department of Molecular Biology, National AIDS Research Institute, Pune, India.
authors: Vijay
authors: V
authors: Nema
authors: Department of Clinical Sciences, National AIDS Research Institute, Pune, India.
authors: Manisha V
authors: MV
authors: Ghate
authors: Department of Clinical Sciences, National AIDS Research Institute, Pune, India.
authors: Raman R
authors: RR
authors: Gangakhedkar
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D003564:Cytidine Deaminase / Q000172:deficiency* / Q000235:genetics; D018450:Disease Progression; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D015658:HIV Infections / Q000453:epidemiology* / Q000235:genetics*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D054643:INDEL Mutation; D007194:India / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D015778:Minor Histocompatibility Antigens / Q000235:genetics; D016133:Polymerase Chain Reaction; D018570:Risk Assessment; D055815:Young Adult
chemical_list: D015778:Minor Histocompatibility Antigens; C495794:APOBEC3B protein, human; D003564:Cytidine Deaminase
keywords: AIDS restriction gene; APOBEC3B; HIV disease progression; acquisition of HIV-1; genetic susceptibility