title: The Necessity of OCT-4 and CDX2 for Early Development and Gene Expression Involved in Differentiation of Inner Cell Mass and Trophectoderm Lineages in Bovine Embryos.
abstract: The functions of POU class 5 transcription factor 1 (Oct-4) and caudal-type homeobox 2 (Cdx2) in the differentiation of the murine inner cell mass (ICM) and trophectoderm (TE) have been described in detail. However, little is known about the roles of OCT-4 and CDX2 in preimplantation bovine embryos. To elucidate their functions during early development in bovine embryos, we performed OCT-4 and CDX2 downregulation using RNA interference. We injected OCT-4- or CDX2-specific short interfering RNAs (siRNAs) into bovine zygotes. The rate of blastocyst development of OCT-4-downregulated embryos was lower compared with uninjected or control siRNA-injected embryos. Gene expression analysis revealed decreased CDX2 and fibroblast growth factor 4 expression in OCT-4-downregulated embryos. CDX2-downregulated embryos developed to the blastocyst stage; however, in most cases, blastocoel formation was delayed. Gene expression analysis revealed decreased GATA3 expression and elevated NANOG expression in CDX2-downregulated embryos. In conclusion, OCT-4 and CDX2 are essential for early development and gene expression involved in differentiation of ICM and TE lineages in bovine embryos.
authors: 1 United Graduate School of Agricultural Sciences, Iwate University , Morioka, Iwate, Japan .
authors: Nobuyuki
authors: N
authors: Sakurai
authors: 1 United Graduate School of Agricultural Sciences, Iwate University , Morioka, Iwate, Japan .
authors: Kazuki
authors: K
authors: Takahashi
authors: 2 Faculty of Agriculture, Iwate University , Morioka, Iwate, Japan .
authors: Natsuko
authors: N
authors: Emura
authors: 3 Animal Research Center , Hokkaido Research Organization, Shintoku, Hokkaido, Japan .
authors: Takashi
authors: T
authors: Fujii
authors: 3 Animal Research Center , Hokkaido Research Organization, Shintoku, Hokkaido, Japan .
authors: Hiroki
authors: H
authors: Hirayama
authors: 3 Animal Research Center , Hokkaido Research Organization, Shintoku, Hokkaido, Japan .
authors: Soichi
authors: S
authors: Kageyama
authors: 1 United Graduate School of Agricultural Sciences, Iwate University , Morioka, Iwate, Japan .|2 Faculty of Agriculture, Iwate University , Morioka, Iwate, Japan .
authors: Tsutomu
authors: T
authors: Hashizume
authors: 1 United Graduate School of Agricultural Sciences, Iwate University , Morioka, Iwate, Japan .|2 Faculty of Agriculture, Iwate University , Morioka, Iwate, Japan .
authors: Ken
authors: K
authors: Sawai
mesh_terms: D000818:Animals; D053624:Blastocyst Inner Cell Mass / Q000166:cytology* / Q000378:metabolism; D000071616:CDX2 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D002454:Cell Differentiation; D019070:Cell Lineage / Q000235:genetics*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism
chemical_list: D000071616:CDX2 Transcription Factor; D050814:Octamer Transcription Factor-3