title: HPLC-DAD-ESI/MS(n) profiling of phenolic compounds from Lathyrus cicera L. seeds.
abstract: Lathyrus cicera L. seeds are of interest for food and feed purposes. Despite the recognized antioxidant activity of the seeds, arising from the phenolic fraction, their phenolic compounds have not been studied in depth yet. Therefore, to determine the phenolics profile of these seeds, a target analysis was performed using high-performance liquid chromatography coupled to photodiode-array detection and electrospray ionization/ion trap mass spectrometry (HPLC-DAD-ESI/MS(n)). Thirty-seven glycosylated flavonoids were identified for the first time in the seeds of this species and, according to their MS fragmentation, clustered in flavonol-3-O-di-/tri-glycosides-7-O-rhamnosides and other flavonol-glycosides, and flavonol-3-O-(cinnamoyl)glycoside-7-O-rhamnosides, flavonol-3-O-(dihydrophaseoyl, cinnamoyl)glycoside-7-O-rhamnosides and flavonol-3-O-(malonyl)glycoside-7-O-rhamnosides. Glycosides of kaempferol were the main flavonoids found (10 non-acylated and 21 acylated), followed by those of quercetin (3) and those of isorhamnetin, apigenin and luteolin (1). The most abundant flavonols were identified as kaempferol-3-O-(2-hexosyl)hexoside-7-O-rhamnosides. The methodology used allowed to increase the knowledge on a relevant phytochemical class of seeds from L. cicera.
authors: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain. Electronic address: federico@cebas.csic.es.
authors: F
authors: F
authors: Ferreres
authors: REQUIMTE/LAQV, ICBAS, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal.
authors: S C Q
authors: SCQ
authors: Magalhães
authors: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain.
authors: A
authors: A
authors: Gil-Izquierdo
authors: REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal.
authors: P
authors: P
authors: Valentão
authors: REQUIMTE/LAQV, ICBAS, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal.
authors: A R J
authors: ARJ
authors: Cabrita
authors: REQUIMTE/LAQV, ICBAS, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal.
authors: A J M
authors: AJM
authors: Fonseca
authors: REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal. Electronic address: pandrade@ff.up.pt.
authors: P B
authors: PB
authors: Andrade
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D044948:Flavonols / Q000032:analysis; D006027:Glycosides / Q000032:analysis*; D006031:Glycosylation; D006868:Hydrolysis; D044949:Kaempferols / Q000032:analysis*; D027807:Lathyrus / Q000737:chemistry*; D013058:Mass Spectrometry; D010636:Phenols / Q000032:analysis; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D005419:Flavonoids; D044948:Flavonols; D006027:Glycosides; D044949:Kaempferols; D010636:Phenols; C047368:3-methylquercetin; C006552:kaempferol; D011794:Quercetin
keywords: HPLC-DAD-ESI/MS(n); Lathyrus cicera L.; Polyphenols; Seeds