title: Polymorphisms in androgen metabolism genes AR, CYP1B1, CYP19, and SRD5A2and prostate cancer risk and aggressiveness in Bulgarian patients.
abstract: OBJECTIVE
  The aim of our study was to elucidate the role of polymorphisms in AR, CYP1B1, CYP19, and SRD5A2 genes for prostate cancer (PC) development in Bulgarian patients.
  
  
  METHODS
  We genotyped 246 PC patients and 261 controls (155 with benign prostate hyperplasia and 107 healthy population controls) using direct sequencing, PCR-RFLP, SSCP, and fragment analysis.
  
  
  RESULTS
  The allele and genotype frequencies of most of the studied variants did not differ significantly between cases and controls. Increased frequencies of the C/C genotype and C allele of rs1056837 in CYP1B1, and genotype 7/8 of the (TTTA)n repeat polymorphism in CYP19, were observed in patients in comparison with controls.The 8/9 and the 7/12 genotypes of (TTTA)n in CYP19 showed suggestive evidence for association with decreased prostate cancer risk and the risk for aggressive disease, respectively. The haplotype analysis revealed 2 CYP1B1 haplotypes associated with PC risk reduction.
  
  
  CONCLUSIONS
  Some CYP1B1 haplotypes and genotypes of the CYP19 (TTTA)n repeat appeared to be associated with disease risk or aggressiveness in Bulgarian PC patients. In contrast, the SRD5A2 polymorphisms (V89L and (TA)n repeat), the CAG repeat in AR, and the Arg264Cys variant in CYP19A1 are most likely not implicated in prostate carcinogenesis.
authors: Department of Medical Chemistry and Biochemistry and Molecular Medicine Center, Medical University of Sofia, Sofia, Bulgaria.
authors: Darina
authors: D
authors: Kachakova
authors: Department of Medical Chemistry and Biochemistry and Molecular Medicine Center, Medical University of Sofia, Sofia, Bulgaria.
authors: Atanaska
authors: A
authors: Mitkova
authors: Department of Urology, Medical University of Sofia, Clinic of Urology, Alexandrovska University Hospital, Sofia, Bulgaria.
authors: Elenko
authors: E
authors: Popov
authors: Department of Medical Chemistry and Biochemistry and Molecular Medicine Center, Medical University of Sofia, Sofia, Bulgaria.
authors: Olga
authors: O
authors: Beltcheva
authors: Department of General and Clinical Pathology, Medical University of Sofia, General and Clinical Pathology Clinic,Alexandrovska University Hospital, Sofia, Bulgaria.
authors: Alexandrina
authors: A
authors: Vlahova
authors: Department of General and Clinical Pathology, Medical University of Sofia, General and Clinical Pathology Clinic,Alexandrovska University Hospital, Sofia, Bulgaria.
authors: Tihomir
authors: T
authors: Dikov
authors: Department of General and Clinical Pathology, Medical University of Sofia, General and Clinical Pathology Clinic,Alexandrovska University Hospital, Sofia, Bulgaria.
authors: Svetlana
authors: S
authors: Christova
authors: Department of Medical Chemistry and Biochemistry and Molecular Medicine Center, Medical University of Sofia, Sofia, Bulgaria.
authors: Vanio
authors: V
authors: Mitev
authors: Department of Urology, Medical University of Sofia, Clinic of Urology, Alexandrovska University Hospital, Sofia, Bulgaria.
authors: Chavdar
authors: C
authors: Slavov
authors: Department of Medical Chemistry and Biochemistry and Molecular Medicine Center, Medical University of Sofia, Sofia, Bulgaria.
authors: Radka
authors: R
authors: Kaneva
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; D001141:Aromatase; D016022:Case-Control Studies; D065633:Cytochrome P-450 CYP1B1; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008565:Membrane Proteins; D011110:Polymorphism, Genetic; D011471:Prostatic Neoplasms; D011944:Receptors, Androgen
chemical_list: D008565:Membrane Proteins; D011944:Receptors, Androgen; D001141:Aromatase; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; C548468:SRD5A2 protein, human
keywords: AR; Association study; CYP19; CYP1B1; SRD5A2; polymorphisms; prostate cancer