title: Nocardiajiangsuensis sp. nov., an actinomycete isolated from coastal soil.
abstract: A novel actinomycete strain, designated strain KLBMP S0027T, was isolated from a coastal soil collected from the coastal region of Lianyungang, Jiangsu Province, in east China, and was studied in detail for its taxonomic position. Comparative 16S rRNA gene sequence analysis showed that this strain belonged to the genus Nocardia and was most closely related to Nocardia harenae WS-26T (98.5 %), Nocardiaasiatica NBRC 100129T (98.5 %), Nocardiaabscessus NBRC 100374T (98.2 %), Nocardia brasiliensis NBRC 14402T (98.2 %) and Nocardiacyriacigeorgica NBRC 100375T (98 %), respectively. The strain showed a combination of chemotaxonomic and morphological properties typical of the genus Nocardia. The cell wall contained meso-diaminopimelic acid (type IV), and whole-cell sugars were arabinose, galactose, glucose and ribose. Strain KLBMP S0027T contained MK-8(H4ω-cycl) as the predominant menaquinone; C16 : 0, C18 : 1ω9c, C18 : 0 10-methyl (TBSA) and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) were the major cellular fatty acids. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, one unknown glycolipid and two unknown lipids. Mycolic acids were detected. The G+C content of the DNA was 70.5 %. However, a combination of DNA-DNA hybridization and phenotypic data demonstrated that strain KLBMP S0027T could be clearly distinguished from the type strain of the most closely related species, N. harenae WS-26T. On the basis of the data presented from a polyphasic study, it was evident that this strain should be assigned to a novel species of the genus Nocardia, for which the name Nocardia jiangsuensis sp. nov. is proposed. The type strain is KLBMP S0027T (=CGMCC 4.7330T=KCTC 39691T).
authors: School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Juan-Luan
authors: JL
authors: Bai
authors: School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Yu
authors: Y
authors: Wang
authors: School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Sheng
authors: S
authors: Qin
authors: School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Peng
authors: P
authors: Ding
authors: School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Ke
authors: K
authors: Xing
authors: School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Bo
authors: B
authors: Yuan
authors: School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Cheng-Liang
authors: CL
authors: Cao
authors: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Ying
authors: Y
authors: Huang
authors: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors: Yu-Qin
authors: YQ
authors: Zhang
authors: School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors: Ji-Hong
authors: JH
authors: Jiang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D009171:Mycolic Acids / Q000737:chemistry; D009615:Nocardia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D009171:Mycolic Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8; D003960:Diaminopimelic Acid