title: Hypoxia-Inducible Factor-1α Regulates CD55 in Airway Epithelium.
abstract: Airway epithelial CD55 down-regulation occurs in several hypoxia-associated pulmonary diseases, but the mechanism is unknown. Using in vivo and in vitro assays of pharmacologic inhibition and gene silencing, the current study investigated the role of hypoxia-inducible factor (HIF)-1α in regulating airway epithelial CD55 expression. Hypoxia down-regulated CD55 expression on small-airway epithelial cells in vitro, and in murine lungs in vivo; the latter was associated with local complement activation. Treatment with pharmacologic inhibition or silencing of HIF-1α during hypoxia-recovered CD55 expression in small-airway epithelial cells. HIF-1α overexpression or blockade, in vitro or in vivo, down-regulated CD55 expression. Collectively, these data show a key role for HIF-1α in regulating the expression of CD55 on airway epithelium.
authors: 1 Department of Microbiology/Immunology.|2 Center for Immunobiology.
authors: Pankita H
authors: PH
authors: Pandya
authors: 2 Center for Immunobiology.
authors: Amanda J
authors: AJ
authors: Fisher
authors: 2 Center for Immunobiology.
authors: Elizabeth A
authors: EA
authors: Mickler
authors: 3 Department of Pathology.
authors: Constance J
authors: CJ
authors: Temm
authors: 3 Department of Pathology.
authors: Kelsey P
authors: KP
authors: Lipking
authors: 2 Center for Immunobiology.
authors: Adam
authors: A
authors: Gracon
authors: 1 Department of Microbiology/Immunology.|4 Department of Medicine, and.
authors: Katia
authors: K
authors: Rothhaar
authors: 3 Department of Pathology.
authors: George E
authors: GE
authors: Sandusky
authors: 5 Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana.
authors: Mary
authors: M
authors: Murray
authors: 5 Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana.
authors: Karen
authors: K
authors: Pollok
authors: 5 Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana.
authors: Jamie
authors: J
authors: Renbarger
authors: 1 Department of Microbiology/Immunology.|2 Center for Immunobiology.
authors: Janice S
authors: JS
authors: Blum
authors: 2 Center for Immunobiology.|4 Department of Medicine, and.
authors: Tim
authors: T
authors: Lahm
authors: 1 Department of Microbiology/Immunology.|2 Center for Immunobiology.|4 Department of Medicine, and.
authors: David S
authors: DS
authors: Wilkes
mesh_terms: D000600:Amino Acids, Dicarboxylic / Q000494:pharmacology; D000818:Animals; D018958:CD55 Antigens / Q000378:metabolism*; D015687:Cell Hypoxia / Q000187:drug effects; D003167:Complement Activation / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism*; D020868:Gene Silencing / Q000187:drug effects; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL
chemical_list: D000600:Amino Acids, Dicarboxylic; D018958:CD55 Antigens; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C040947:oxalylglycine
keywords: complement regulatory proteins; hypoxia; lung