title: Polymorphism of the renalase gene in gestational diabetes mellitus.
abstract: Renalase is considered as a novel candidate gene for type 2 diabetes. In this study, we aimed to investigate the relationship of serum renalase and two single nucleotide polymorphisms with gestational diabetes mellitus. One hundred and ninety-eight normotensive pregnant females (n = 99 gestational diabetes mellitus; n = 99 euglycemic pregnant controls) were classified according to the International Association of the Diabetes and Pregnancy Study criteria. Fasting and 2-h post glucose load blood levels and anthropometric assessment was performed. Serum renalase was measured using enzyme-linked immunosorbent assay, whereas DNA samples were genotyped for renalase single nucleotide polymorphisms rs2576178 and rs10887800 using Polymerase chain reaction-Restriction fragment length polymorphism method. In an age-matched case control study, no difference was observed in the serum levels of renalase (p > 0.05). The variant rs10887800 showed an association with gestational diabetes mellitus and remained significant after multiple adjustments (p < 0.05), whereas rs2576178 showed weak association (p = 0.030) that was lost after multiple adjustments (p = 0.09). We inferred a modest association of the rs10887800 polymorphism with gestational diabetes. Although gestational diabetes mellitus is self-reversible, yet presence of this minor G allele might predispose to metabolic syndrome phenotypes in near the future.
authors: Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan. sadia.fatima@aku.edu.
authors: Syeda Sadia
authors: SS
authors: Fatima
authors: Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan.
authors: Zehra
authors: Z
authors: Jamil
authors: Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan.
authors: Faiza
authors: F
authors: Alam
authors: Medical College, Aga Khan University, Karachi, Pakistan.
authors: Hajira Zafar
authors: HZ
authors: Malik
authors: Medical College, Aga Khan University, Karachi, Pakistan.
authors: Sarosh Irfan
authors: SI
authors: Madhani
authors: Medical College, Aga Khan University, Karachi, Pakistan.
authors: Muhammad Saad
authors: MS
authors: Ahmad
authors: Medical College, Aga Khan University, Karachi, Pakistan.
authors: Tayyab
authors: T
authors: Shabbir
authors: Medical College, Aga Khan University, Karachi, Pakistan.
authors: Muhammed Noman
authors: MN
authors: Rehmani
authors: Medical College, Aga Khan University, Karachi, Pakistan.
authors: Amna
authors: A
authors: Rabbani
mesh_terms: D000328:Adult; D000483:Alleles; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D016022:Case-Control Studies; D015331:Cohort Studies; D016640:Diabetes, Gestational / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008995:Monoamine Oxidase / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D010154:Pakistan; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D015430:Weight Gain; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D008995:Monoamine Oxidase; C521557:renalase
keywords: BMI; Gestational diabetes; Metabolic syndrome; Obesity; Renalase