title: Isolation and Characterization of a Highly Mutated Chinese Isolate of Enterovirus B84 from a Patient with Acute Flaccid Paralysis.
abstract: Enterovirus B84 (EV-B84) is a newly identified serotype within the species Enterovirus B (EV-B). To date, only ten nucleotide sequences of EV-B84 are published and only one full-length genome sequence (the prototype strain) is available in the GenBank database. Here, a highly mutated EV-B84 (strain AFP452/GD/CHN/2004) was recovered from a patient with acute flaccid paralysis in the Guangdong province of China in 2004 making this the first report of EV-B84 in China. Sequence comparison and phylogenetic dendrogram analysis revealed high variation from the global EV-B84 strains (African and Indian strains) and frequent intertypic recombination in the non-structural protein region, suggesting high genetic diversity in EV-B84. The Chinese EV-B84 strain, apparently evolving independently of the other ten strains, strongly suggests that the EV-B84 strain has been circulating for many years. However, the extremely low isolation rate suggests that it is not a prevalent EV serotype in China or worldwide. This study provides valuable information about the molecular epidemiology of EV-B84 in China, and will be helpful in future studies to understand the association of EV-B84 with neurological disorders; it also helps expand the number of whole virus genome sequences of EV-B84 in the GenBank database.
authors: Guangdong Provincial Center for Disease Control and Prevention, No. 160, Qunxian Road, Panyu District, Guangzhou, China.
authors: Huanying
authors: H
authors: Zheng
authors: WHO WPRO Regional Polio Reference Laboratory, Key Laboratory of Medical Virology, National Health and Family Planning Commission of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
authors: Yong
authors: Y
authors: Zhang
authors: Guangdong Provincial Center for Disease Control and Prevention, No. 160, Qunxian Road, Panyu District, Guangzhou, China.
authors: Leng
authors: L
authors: Liu
authors: Guangdong Provincial Center for Disease Control and Prevention, No. 160, Qunxian Road, Panyu District, Guangzhou, China.|Guangdong Provincial Institution of Public Health, Guangzhou, China.
authors: Jing
authors: J
authors: Lu
authors: Guangdong Provincial Center for Disease Control and Prevention, No. 160, Qunxian Road, Panyu District, Guangzhou, China.
authors: Xue
authors: X
authors: Guo
authors: Guangdong Provincial Center for Disease Control and Prevention, No. 160, Qunxian Road, Panyu District, Guangzhou, China.
authors: Hui
authors: H
authors: Li
authors: Guangdong Provincial Center for Disease Control and Prevention, No. 160, Qunxian Road, Panyu District, Guangzhou, China.
authors: Hanri
authors: H
authors: Zeng
authors: Guangdong Provincial Center for Disease Control and Prevention, No. 160, Qunxian Road, Panyu District, Guangzhou, China.
authors: Ling
authors: L
authors: Fang
authors: WHO WPRO Regional Polio Reference Laboratory, Key Laboratory of Medical Virology, National Health and Family Planning Commission of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
authors: Wenbo
authors: W
authors: Xu
authors: Guangdong Provincial Center for Disease Control and Prevention, No. 160, Qunxian Road, Panyu District, Guangzhou, China.
authors: Changwen
authors: C
authors: Ke
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D002675:Child, Preschool; D029822:Enterovirus B, Human / Q000235:genetics* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D008297:Male; D009154:Mutation; D010264:Paraplegia / Q000821:virology*; D010802:Phylogeny; D012189:Retrospective Studies; D017422:Sequence Analysis, DNA