title: Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species.
abstract: Production of lignocellulosic butanol has drawn increasing attention. However, currently few microorganisms can produce biofuels, particularly butanol, from lignocellulosic biomass via simultaneous saccharification and fermentation. Here we report discovery of a wild-type, mesophilic Clostridium sp. strain MF28 that ferments xylan to produce butanol (up to 3.2g/L) without the addition of saccharolytic enzymes and without any chemical pretreatments. Application of selective pressure from 2-deoxy-d-glucose facilitated isolation of strain MF28, which exhibits inactivation of genes (gid and ccp genes) responsible for carbon catabolite repression, thus allowing strain MF28 to simultaneously ferment a combination of glucose (30g/L), xylose (15g/L), and arabinose (15g/L) to produce 11.9g/L of butanol. Strain MF28 possesses several unique features: (i) non-sporulating, (ii) no acetone/ethanol, (iii) complete hemicellulose-binding enzymatic domain, and (iv) absence of carbon catabolite repression. These unique characteristics demonstrate the industrial potential of strain MF28 for cost-effective biofuel generation from lignocellulosic biomass.
authors: Department of Civil and Environmental Engineering, National University of Singapore, Singapore 117576, Singapore; Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People's Republic of China.
authors: Tinggang
authors: T
authors: Li
authors: Department of Civil and Environmental Engineering, National University of Singapore, Singapore 117576, Singapore. Electronic address: jianzhong.he@nus.edu.sg.
authors: Jianzhong
authors: J
authors: He
mesh_terms: D001089:Arabinose / Q000378:metabolism*; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology; D000440:Butanols / Q000378:metabolism*; D003013:Clostridium / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*
chemical_list: D056804:Biofuels; D000440:Butanols; D011134:Polysaccharides; C007916:hemicellulose; D001089:Arabinose; D005947:Glucose
keywords: Butanol; Carbon catabolite derepression; Clostridium; No acetone/ethanol formation; Xylan