title: Insights into the fluoride-resistant regulation mechanism of Acidithiobacillus ferrooxidans ATCC 23270 based on whole genome microarrays.
abstract: Acidophilic microorganisms involved in uranium bioleaching are usually suppressed by dissolved fluoride ions, eventually leading to reduced leaching efficiency. However, little is known about the regulation mechanisms of microbial resistance to fluoride. In this study, the resistance of Acidithiobacillus ferrooxidans ATCC 23270 to fluoride was investigated by detecting bacterial growth fluctuations and ferrous or sulfur oxidation. To explore the regulation mechanism, a whole genome microarray was used to profile the genome-wide expression. The fluoride tolerance of A. ferrooxidans cultured in the presence of FeSO4 was better than that cultured with the S(0) substrate. The differentially expressed gene categories closely related to fluoride tolerance included those involved in energy metabolism, cellular processes, protein synthesis, transport, the cell envelope, and binding proteins. This study highlights that the cellular ferrous oxidation ability was enhanced at the lower fluoride concentrations. An overview of the cellular regulation mechanisms of extremophiles to fluoride resistance is discussed.
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China.|Key Laboratory of Biometallurgy, Ministry of Education, Changsha, China.
authors: Liyuan
authors: L
authors: Ma
authors: School of Nuclear Resources Engineering, University of South China, Hengyang, China.|Key Discipline Laboratory for National Defense of Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, China.
authors: Qian
authors: Q
authors: Li
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China.|Key Laboratory of Biometallurgy, Ministry of Education, Changsha, China.
authors: Li
authors: L
authors: Shen
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China.|Key Laboratory of Biometallurgy, Ministry of Education, Changsha, China.
authors: Xue
authors: X
authors: Feng
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China.|Key Laboratory of Biometallurgy, Ministry of Education, Changsha, China.
authors: Yunhua
authors: Y
authors: Xiao
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China.|Key Laboratory of Biometallurgy, Ministry of Education, Changsha, China.
authors: Jiemeng
authors: J
authors: Tao
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China.|Key Laboratory of Biometallurgy, Ministry of Education, Changsha, China.
authors: Yili
authors: Y
authors: Liang
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China.|Key Laboratory of Biometallurgy, Ministry of Education, Changsha, China.
authors: Huaqun
authors: H
authors: Yin
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China. xueduanliu@126.com.|Key Laboratory of Biometallurgy, Ministry of Education, Changsha, China. xueduanliu@126.com.
authors: Xueduan
authors: X
authors: Liu
mesh_terms: D042763:Acidithiobacillus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004734:Energy Metabolism / Q000235:genetics; D005296:Ferrous Compounds / Q000378:metabolism; D005459:Fluorides / Q000633:toxicity*; D020869:Gene Expression Profiling; D023281:Genomics; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction; D013455:Sulfur / Q000378:metabolism
chemical_list: D005296:Ferrous Compounds; D013455:Sulfur; D005459:Fluorides
keywords: A. ferrooxidans ATCC 23270; Expression profiling; Fluoride; Whole genome microarray