title: Nasal delivery of chitosan-coated poly(lactide-co-glycolide)-encapsulated honeybee (Apis mellifera) venom promotes Th 1-specific systemic and local intestinal immune responses in weaned pigs.
abstract: Nasal delivery is a convenient and acceptable route for drug administration, and has been shown to elicit a much more potent local and systemic response compared with other drug delivery routes. We previously demonstrated that rectal administration of poly(lactide-co-glycolide)-encapsulated honeybee venom (P-HBV) could enhance systemic Th 1-specific immune responses. We therefore synthesized chitosan-coated P-HBV (CP-HBV) and then evaluated the immune-boosting efficacy of nasally administered CP-HBV on systemic and local intestinal immunity compared with non-chitosan-coated P-HBV. The nasally delivered CP-HBV effectively enhanced Th 1-specific responses, eliciting a significant increase in the CD3(+)CD4(+)CD8(-) Th cell population, lymphocyte proliferation capacity, and expression of Th 1 cytokines (IFN-γ, IL-12, and IL-2) in peripheral blood mononuclear cells. Furthermore, these immune-boosting effects persisted up to 21days post CP-HBV administration. Nasal administration of CP-HBV also led to an increase of not only the CD4(+) Th 1 and IFN-γ secreting CD4(+) Th 1 cell population but also Th 1-specific cytokines and transcription factors, including IL-12, IFN-γ, STAT4, and T-bet, in isolated mononuclear cells from the spleen and ileum.
authors: Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors: Jin-A
authors: JA
authors: Lee
authors: Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors: Yun-Mi
authors: YM
authors: Kim
authors: Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors: Tae-Hoon
authors: TH
authors: Kim
authors: Department of Veterinary Obstetrics, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors: Sang-Ho
authors: SH
authors: Lee
authors: College of Pharmacy and Institute of Drug Research & Development, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors: Cho-A
authors: CA
authors: Lee
authors: College of Pharmacy and Institute of Drug Research & Development, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors: Cheong-Weon
authors: CW
authors: Cho
authors: Wissen Co., Ltd., #410 Bio Venture Town, 461-8, Daejeon 305-811, Republic of Korea.
authors: Jong-Woon
authors: JW
authors: Jeon
authors: Wissen Co., Ltd., #410 Bio Venture Town, 461-8, Daejeon 305-811, Republic of Korea.
authors: Jin-Kyu
authors: JK
authors: Park
authors: Department of Companion & Laboratory Animal Science, Kongju National University, Yesan, Chungnam, 304-702, Republic of Korea.
authors: Sang-Ki
authors: SK
authors: Kim
authors: Department of Pulmonary Immunology, Center for Pulmonary and Infectious Diseases Control, University of Texas Health Science Center at Tyler, TX 75708, USA. Electronic address: skulljung@gmail.com.
authors: Bock-Gie
authors: BG
authors: Jung
authors: Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea. Electronic address: bjlee@chonnam.ac.kr.
authors: Bong-Joo
authors: BJ
authors: Lee
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001514:Bee Venoms / Q000008:administration & dosage* / Q000276:immunology*; D001516:Bees / Q000276:immunology*; D048271:Chitosan; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D016503:Drug Delivery Systems; D018928:Immunity, Mucosal; D007413:Intestinal Mucosa / Q000276:immunology; D011098:Polyglactin 910; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034421:Sus scrofa / Q000235:genetics / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D014157:Transcription Factors / Q000235:genetics; D015854:Up-Regulation
chemical_list: D001514:Bee Venoms; D016207:Cytokines; D012333:RNA, Messenger; D014157:Transcription Factors; D011098:Polyglactin 910; D048271:Chitosan
keywords: Chitosan; Honeybee venom; Immune boosting effect; Mucoadhesive nanoparticle; Poly(lactide-co-dlycolide)