title: A new densovirus in cerebrospinal fluid from a case of anti-NMDA-receptor encephalitis.
abstract: We characterized the genome of a densovirus, tentatively called human CSF-associated densovirus 1 (HuCSFDV1), in cerebrospinal fluid (CSF) from a human case of encephalitis with antibodies against the N-methyl D-aspartate receptor. The presence of the viral genome in CSF was independently confirmed. This virus, which is proposed to be a member of a new species in the genus Iteradensovirus of the subfamily Densovirinae, showed the typical two ORFs encoding nonstructural and structural proteins with low-level identities of 22 and 16 % to the closest known densovirus relative. No other eukaryotic viral sequences were detected using deep sequencing. The replication and pathogenicity in humans of this virus, which belongs to a viral subfamily whose members are only known to replicate in invertebrates, remain to be demonstrated. Alternative explanations for the detection of densovirus DNA in CSF are discussed.
authors: Blood Systems Research Institute, San Francisco, CA, 94118, USA.|Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA, 94118, USA.
authors: Tung Gia
authors: TG
authors: Phan
authors: Children's Hospital Colorado and University of Colorado School of Medicine, Aurora, CO, USA.
authors: Kevin
authors: K
authors: Messacar
authors: Children's Hospital Colorado and University of Colorado School of Medicine, Aurora, CO, USA.
authors: Samuel R
authors: SR
authors: Dominguez
authors: Blood Systems Research Institute, San Francisco, CA, 94118, USA.|Institute of Tropical Medicine, School of Medicine, University of São Paulo, São Paulo, Brazil.
authors: Antonio Charlys
authors: AC
authors: da Costa
authors: Blood Systems Research Institute, San Francisco, CA, 94118, USA.
authors: Xutao
authors: X
authors: Deng
authors: Blood Systems Research Institute, San Francisco, CA, 94118, USA. delwarte@medicine.ucsf.edu.|Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA, 94118, USA. delwarte@medicine.ucsf.edu.
authors: Eric
authors: E
authors: Delwart
mesh_terms: D060426:Anti-N-Methyl-D-Aspartate Receptor Encephalitis / Q000473:pathology*; D002555:Cerebrospinal Fluid / Q000821:virology*; D002648:Child; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D017990:Densovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D016679:Genome, Viral; D006801:Humans; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D004279:DNA, Viral