title: Controllable in-situ cell electroporation with cell positioning and impedance monitoring using micro electrode array.
abstract: This paper reports a novel microarray chip for in-situ, real-time and selective electroporation on individual cells integrated with cell positioning and impedance monitoring. An array of quadrupole-electrode units (termed positioning electrodes) and pairs of planar center electrodes located at the centers of each quadrupole-electrode unit were fabricated on the chip. The positioning electrodes are used to trap and position living cells onto the center electrodes based on negative dielectrophoresis (nDEP). The center electrodes are used for in-situ cell electroporation, and also used to measure cell impedance for monitoring cellular dynamics in real time. Controllably selective electroporation and electrical measurement on the cells in array are realized. We present an evidence of selective electroporation through use of fluorescent dyes. Subsequently we use in-situ and real-time impedance measurement to monitor the process, which demonstrates the dynamic behavior of the cell electroporation. Finally, we show the use of this device to perform successful transfection onto individual HeLa cells with vector DNA encoding a green fluorescent.
authors: State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing, 100084, China.
authors: Xiaoliang
authors: X
authors: Guo
authors: State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing, 100084, China.
authors: Rong
authors: R
authors: Zhu
mesh_terms: D017097:Electric Impedance; D004566:Electrodes; D018274:Electroporation / Q000295:instrumentation*; D006367:HeLa Cells; D006801:Humans; D059010:Single-Cell Analysis / Q000379:methods*; D046888:Tissue Array Analysis; D014162:Transfection / Q000295:instrumentation*