title: Shear-wave elasticity measurements of three-dimensional cell cultures for mechanobiology.
abstract: Studying mechanobiology in three-dimensional (3D) cell cultures better recapitulates cell behaviors in response to various types of mechanical stimuli in vivo Stiffening of the extracellular matrix resulting from cell remodeling potentiates many pathological conditions, including advanced cancers. However, an effective tool for measuring the spatiotemporal changes in elastic properties of such 3D cell cultures without directly contacting the samples has not been reported previously. We describe an ultrasonic shear-wave-based platform for quantitatively evaluating the spatiotemporal dynamics of the elasticity of a matrix remodeled by cells cultured in 3D environments. We used this approach to measure the elasticity changes of 3D matrices grown with highly invasive lung cancer cells and cardiac myoblasts, and to delineate the principal mechanism underlying the stiffening of matrices remodeled by these cells. The described approach can be a useful tool in fields investigating and manipulating the mechanotransduction of cells in 3D contexts, and also has potential as a drug-screening platform.
authors: Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan.|Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan.|Department of Rehabilitation, National Taiwan University Hospital, Taipei 10002, Taiwan.
authors: Po-Ling
authors: PL
authors: Kuo
authors: Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan.
authors: Ching-Che
authors: CC
authors: Charng
authors: Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan.
authors: Po-Chen
authors: PC
authors: Wu
authors: Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan paichi@ntu.edu.tw.|Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors: Pai-Chi
authors: 0000-0001-7603-7852
authors: PC
authors: Li
mesh_terms: D000818:Animals; D016880:Anisotropy; D001703:Biophysics / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D003094:Collagen / Q000494:pharmacology; D004548:Elasticity; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology; D040542:Mechanotransduction, Cellular; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects; D009206:Myocardium / Q000166:cytology; D051381:Rats; D012212:Rheology; D033081:Shear Strength; D062211:Spatio-Temporal Analysis; D013696:Temperature
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003094:Collagen
keywords: Matrix elasticity; Matrix remodeling; Three-dimensional