title: Biomechanical Characterization of Cardiomyocyte Using PDMS Pillar with Microgrooves.
abstract: This paper describes the surface-patterned polydimethylsiloxane (PDMS) pillar arrays for enhancing cell alignment and contraction force in cardiomyocytes. The PDMS micropillar (μpillar) arrays with microgrooves (μgrooves) were fabricated using a unique micro-mold made using SU-8 double layer processes. The spring constant of the μpillar arrays was experimentally confirmed using atomic force microscopy (AFM). After culturing cardiac cells on the two different types of μpillar arrays, with and without grooves on the top of μpillar, the characteristics of the cardiomyocytes were analyzed using a custom-made image analysis system. The alignment of the cardiomyocytes on the μgrooves of the μpillars was clearly observed using a DAPI staining process. The mechanical force generated by the contraction force of the cardiomyocytes was derived from the displacement of the μpillar arrays. The contraction force of the cardiomyocytes aligned on the μgrooves was 20% higher than that of the μpillar arrays without μgrooves. The experimental results prove that applied geometrical stimulus is an effective method for aligning and improving the contraction force of cardiomyocytes.
authors: MEMS and Nanotechnology Laboratory, Mechanical Engineering, Chonnam National University, Gwangju 61186, Korea. nominerdene16@yahoo.com.
authors: Nomin-Erdene
authors: NE
authors: Oyunbaatar
authors: MEMS and Nanotechnology Laboratory, Mechanical Engineering, Chonnam National University, Gwangju 61186, Korea. cinsea@naver.com.
authors: Deok-Hyu
authors: DH
authors: Lee
authors: MEMS and Nanotechnology Laboratory, Mechanical Engineering, Chonnam National University, Gwangju 61186, Korea. swatipatil39@gmail.com.
authors: Swati J
authors: SJ
authors: Patil
authors: Department of Biological Sciences, Chonnam National University, Gwangju 61186, Korea. eungsam.kim@jnu.ac.kr.
authors: Eung-Sam
authors: ES
authors: Kim
authors: MEMS and Nanotechnology Laboratory, Mechanical Engineering, Chonnam National University, Gwangju 61186, Korea. mems@jnu.ac.kr.
authors: Dong-Weon
authors: DW
authors: Lee
mesh_terms: D001696:Biomechanical Phenomena; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D007211:Indoles / Q000737:chemistry; D018625:Microscopy, Atomic Force; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D004129:Dimethylpolysiloxanes; D007211:Indoles; C007293:DAPI; C013830:baysilon
keywords: cardiomyocyte; contraction force; drug screening; microgrooves; polydimethylsiloxane pillar