title: A Quick-responsive DNA Nanotechnology Device for Bio-molecular Homeostasis Regulation.
abstract: Physiological processes such as metabolism, cell apoptosis and immune responses, must be strictly regulated to maintain their homeostasis and achieve their normal physiological functions. The speed with which bio-molecular homeostatic regulation occurs directly determines the ability of an organism to adapt to conditional changes. To produce a quick-responsive regulatory system that can be easily utilized for various types of homeostasis, a device called nano-fingers that facilitates the regulation of physiological processes was constructed using DNA origami nanotechnology. This nano-fingers device functioned in linked open and closed phases using two types of DNA tweezers, which were covalently coupled with aptamers that captured specific molecules when the tweezer arms were sufficiently close. Via this specific interaction mechanism, certain physiological processes could be simultaneously regulated from two directions by capturing one biofactor and releasing the other to enhance the regulatory capacity of the device. To validate the universal application of this device, regulation of the homeostasis of the blood coagulant thrombin was attempted using the nano-fingers device. It was successfully demonstrated that this nano-fingers device achieved coagulation buffering upon the input of fuel DNA. This nano-device could also be utilized to regulate the homeostasis of other types of bio-molecules.
authors: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Innovation Base of Life Science and Technology, Qiming College, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.
authors: Songlin
authors: S
authors: Wu
authors: Innovation Base of Life Science and Technology, Qiming College, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Department of Nanomedicine and Biopharmaceutics, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Bei Shizhang Advanced Class of Life Science Research, co-founded by Huazhong University of Science and Technology, Wuhan, 430074, P.R.China, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P.R.China and University of Chinese Academy of Sciences, Beijing, P.R.China.
authors: Pei
authors: P
authors: Wang
authors: Innovation Base of Life Science and Technology, Qiming College, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Department of Nanomedicine and Biopharmaceutics, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.
authors: Chen
authors: C
authors: Xiao
authors: Innovation Base of Life Science and Technology, Qiming College, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Bei Shizhang Advanced Class of Life Science Research, co-founded by Huazhong University of Science and Technology, Wuhan, 430074, P.R.China, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P.R.China and University of Chinese Academy of Sciences, Beijing, P.R.China.|National Education Base of Bioscience, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.
authors: Zheng
authors: Z
authors: Li
authors: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Innovation Base of Life Science and Technology, Qiming College, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Bei Shizhang Advanced Class of Life Science Research, co-founded by Huazhong University of Science and Technology, Wuhan, 430074, P.R.China, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P.R.China and University of Chinese Academy of Sciences, Beijing, P.R.China.
authors: Bing
authors: B
authors: Yang
authors: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Innovation Base of Life Science and Technology, Qiming College, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.
authors: Jieyang
authors: J
authors: Fu
authors: Innovation Base of Life Science and Technology, Qiming College, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Bei Shizhang Advanced Class of Life Science Research, co-founded by Huazhong University of Science and Technology, Wuhan, 430074, P.R.China, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P.R.China and University of Chinese Academy of Sciences, Beijing, P.R.China.|National Education Base of Bioscience, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.
authors: Jing
authors: J
authors: Chen
authors: Innovation Base of Life Science and Technology, Qiming College, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Bei Shizhang Advanced Class of Life Science Research, co-founded by Huazhong University of Science and Technology, Wuhan, 430074, P.R.China, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P.R.China and University of Chinese Academy of Sciences, Beijing, P.R.China.|National Education Base of Bioscience, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.
authors: Neng
authors: N
authors: Wan
authors: Department of Nanomedicine and Biopharmaceutics, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Bei Shizhang Advanced Class of Life Science Research, co-founded by Huazhong University of Science and Technology, Wuhan, 430074, P.R.China, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P.R.China and University of Chinese Academy of Sciences, Beijing, P.R.China.|Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.
authors: Cong
authors: C
authors: Ma
authors: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.
authors: Maoteng
authors: M
authors: Li
authors: Department of Nanomedicine and Biopharmaceutics, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.
authors: Xiangliang
authors: X
authors: Yang
authors: Innovation Base of Life Science and Technology, Qiming College, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Bei Shizhang Advanced Class of Life Science Research, co-founded by Huazhong University of Science and Technology, Wuhan, 430074, P.R.China, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P.R.China and University of Chinese Academy of Sciences, Beijing, P.R.China.|Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.|Student Affair Office, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R.China.
authors: Yi
authors: Y
authors: Zhan
mesh_terms: D001696:Biomechanical Phenomena; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006706:Homeostasis; D006801:Humans; D036103:Nanotechnology / Q000295:instrumentation*; D013917:Thrombin / Q000378:metabolism*
chemical_list: D004247:DNA; D013917:Thrombin