title: Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs.
abstract: The intraocular route of drug administration enables the delivery of high concentrations of therapeutic drugs, while minimizing their systemic absorption. Several drugs are administered into the anterior chamber or vitreous, and the intraocular injection has been effective in curing various intraocular diseases. Rabbit eyes have been widely used for ophthalmic research, as the animal is easy to handle and economical compared to other mammals, and the size of a rabbit eye is similar to that of a human eye. Using a 30 G needle, drugs can be injected into the intracameral and intravitreal spaces of rabbit eyes. The eyeballs are then frozen until analysis, and can be divided into the aqueous humor, vitreous, and retina/choroid. The vitreous and retina/choroid samples can be homogenized and solubilized before analysis. Then, immunoassays can be performed to measure the concentrations of intraocular drugs in each compartment. Appropriate pharmacokinetic models can be used to calculate several parameters, such as the half-life and maximum concentration of the drug. Rabbit eyes can be a good model for pharmacokinetic studies of intraocular drugs.
authors: Department of Ophthalmology, Seoul National University Bundang Hospital; Department of Ophthalmology, College of Medicine, Seoul National University; Department of Ophthalmology, Hanyang University Hospital.
authors: Seong Joon
authors: SJ
authors: Ahn
authors: Department of Ophthalmology, Seoul National University Bundang Hospital.
authors: Hye Kyoung
authors: HK
authors: Hong
authors: Department of Ophthalmology, Seoul National University Bundang Hospital.
authors: Young Mi
authors: YM
authors: Na
authors: Department of Ophthalmology, Seoul National University Bundang Hospital; Department of Ophthalmology, College of Medicine, Seoul National University.
authors: Sang Jun
authors: SJ
authors: Park
authors: Department of Ophthalmology, Seoul National University Bundang Hospital; Department of Ophthalmology, College of Medicine, Seoul National University; Department of Ophthalmology, Seoul Metropolitan Government-Seoul National University Boramae Medical Center.
authors: Jeeyun
authors: J
authors: Ahn
authors: Department of Clinical Pharmacology, Seoul National University Hospital.
authors: Jaeseong
authors: J
authors: Oh
authors: Department of Clinical Pharmacology, Seoul National University Bundang Hospital.
authors: Jae Yong
authors: JY
authors: Chung
authors: Department of Ophthalmology, Seoul National University Bundang Hospital; Department of Ophthalmology, College of Medicine, Seoul National University.
authors: Kyu Hyung
authors: KH
authors: Park
authors: Department of Ophthalmology, Seoul National University Bundang Hospital; Department of Ophthalmology, College of Medicine, Seoul National University; sejoon1@snu.ac.kr.
authors: Se Joon
authors: SJ
authors: Woo
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000187:drug effects*; D002829:Choroid / Q000187:drug effects*; D006801:Humans; D056965:Injections, Intraocular; D004364:Pharmaceutical Preparations; D010599:Pharmacokinetics; D011817:Rabbits; D012160:Retina / Q000187:drug effects*; D014822:Vitreous Body / Q000187:drug effects*
chemical_list: D004364:Pharmaceutical Preparations