title: Salmonella Bacterial Monotherapy Reduces Autochthonous Prostate Tumor Burden in the TRAMP Mouse Model.
abstract: Attenuated Salmonella typhimurium injected in the circulatory system of mammals selectively targets tumors. Using weekly intraperitoneal injections of attenuated Salmonella strain CRC2631, we tested for regression and/or inhibition of tumor development in the TRAMP prostate tumor mouse model, which utilizes SV40 early region expression for autochthonous formation of prostate tumors that progress into metastatic, poorly differentiated prostatic carcinomas in an immunocompetent murine model. Thirteen weekly intraperitoneal administrations of 105-107 CFU CRC2631 into 10 week old mice were well tolerated by the TRAMP model. Sacrifice and histological analysis of TRAMP prostates at 22 weeks indicated that Salmonella monotherapy at administrated levels decrease visible tumor size (>29%) but did not significantly inhibit previously described SV40 expression-driven TRAMP tumor progression to undifferentiated carcinomas when histologically examined. In conclusion, this work demonstrates baseline results for CRC2631 Salmonella monotherapy using the immunocompetent TRAMP prostate tumor model in preparation for study of combination therapies that resolve autochthonously generated TRAMP prostate tumors, further reduce tumor size, or inhibit prostate tumor progression.
authors: Cancer Research Center, Columbia, Missouri, United States of America.|Division of Biological Sciences, University of Missouri, Columbia, Missouri, United States of America.
authors: Robert A
authors: RA
authors: Kazmierczak
authors: Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States of America.
authors: Bettina
authors: B
authors: Gentry
authors: Cancer Research Center, Columbia, Missouri, United States of America.|Division of Biological Sciences, University of Missouri, Columbia, Missouri, United States of America.
authors: Tyler
authors: T
authors: Mumm
authors: Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States of America.
authors: Heide
authors: H
authors: Schatten
authors: Cancer Research Center, Columbia, Missouri, United States of America.|Division of Biological Sciences, University of Missouri, Columbia, Missouri, United States of America.
authors: Abraham
authors: A
authors: Eisenstark
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology / Q000382:microbiology* / Q000473:pathology*; D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D018450:Disease Progression; D006801:Humans; D007114:Immunization; D007267:Injections; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D060787:Neoplasm Grading; D011471:Prostatic Neoplasms / Q000276:immunology / Q000382:microbiology* / Q000473:pathology*; D012486:Salmonella typhimurium / Q000502:physiology*; D047368:Tumor Burden