title: The action and mechanism of myrislignan on A549 cells in vitro and in vivo.
abstract: Myrislignan is a natural compound with little pharmacological study. In our investigation, we investigated the effect of myrislignan in the induction of apoptosis in A549 cells in vitro and in vivo. Myrislignan inhibited the proliferation of A549 cells in a dose- and time-dependent manner assayed by MTT. In addition, Hoechst flow cytometry showed that myrislignan significantly induced apoptosis and cell cycle arrest in A549 cells. The apoptosis and anti-cell proliferation was mediated by the activation of mitogen-activated protein kinase and the inhibition of epidermal growth factor receptor signal pathway, change of mitochondrial membrane potential, the releasing of c-Myc, the downregulation of the level of the anti-apoptotic protein Bcl-2, and the upregulation of the level of the pro-apoptotic protein Bax. In conclusion, those results reveal a potential mechanism for the anti-cancer effect of myrislignan on human lung cancer, while suggesting that myrislignan may be a promising compound for the treatment of lung cancer.
authors: Department of Traditional Chinese Medicine, HuaDong Hospital, FuDan University, Shanghai, 200040, People's Republic of China.
authors: XinGang
authors: X
authors: Lu
authors: Department of Tumor, BaoShan Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, 201999, People's Republic of China.
authors: Liu
authors: L
authors: Yang
authors: Department of Traditional Chinese Medicine, RuiJin Hospital, JiaoTong University School of Medicine, Shanghai, 200025, People's Republic of China.
authors: JingXian
authors: J
authors: Chen
authors: Department of Pulmonary, HuaDong Hospital, FuDan University, Shanghai, 200040, People's Republic of China.
authors: JiAn
authors: J
authors: Zhou
authors: Department of Pulmonary, HuaDong Hospital, FuDan University, Shanghai, 200040, People's Republic of China.
authors: XiaoDan
authors: X
authors: Tang
authors: Department of Pulmonary, HuaDong Hospital, FuDan University, Shanghai, 200040, People's Republic of China.
authors: YingGang
authors: Y
authors: Zhu
authors: Department of Traditional Chinese Medicine, HuaDong Hospital, FuDan University, Shanghai, 200040, People's Republic of China.
authors: HongFu
authors: H
authors: Qiu
authors: Department of Pharmacy, HuaDong Hospital, FuDan University, No. 221, West YanAn Rd, Shanghai, 200040, People's Republic of China. shj421@126.com.
authors: Jie
authors: J
authors: Shen
mesh_terms: D000072283:A549 Cells; D017209:Apoptosis; D001688:Biological Products / Q000494:pharmacology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D007150:Immunohistochemistry; D017705:Lignans / Q000494:pharmacology / Q000627:therapeutic use*; D008175:Lung Neoplasms / Q000188:drug therapy*; D015398:Signal Transduction; D023041:Xenograft Model Antitumor Assays
chemical_list: C531053:2-(4-allyl-2,6-dimethoxyphenoxy)-1-(4-hydroxy-3-methoxyphenyl)propan-1-ol; D001688:Biological Products; D017705:Lignans
keywords: Apoptosis; Cell cycle arrest; EGFR-MAPK; Human lung cancer cells; Myrislignan