title: LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton.
abstract: Reproductive hormones influence breast cancer development and progression. While the actions of sex steroids in this setting are established, tentative evidence suggests that follicle-stimulating hormone (FSH) and luteinizing hormone (LH) may also play a role, yet this remains elusive. We here identify that T-47D breast cancer cells express functional receptors for FSH and LH, and that these hormones regulate breast cancer cell motility and invasion through the control of the actin cytoskeleton and the formation of cortical actin aggregates and focal adhesion complexes. Such actions are mediated by the cytoskeletal controllers Moesin and focal adhesion kinase (FAK). Moesin is recruited rapidly by FSH and LH through a signaling cascade requiring the G protein Gα13and the Rho-associated kinase, ROCK-2. FSH and LH activate FAK via a Gαi/β and c-Src-dependent signaling cascade. Both cascades involve signaling to phosphatidylinositol-3 kinase and Akt. FSH and LH receptors and the related signaling intermediates are necessary for the actions of gonadotrophins on breast cancer cell cytoskeletal rearrangement, migration and invasion. These findings provide original information on the actions of gonadotrophins on breast cancer cells and may have clinical implications for the use of drugs that modulate gonadotrophins in breast cancer patients.
authors: Molecular and Cellular Gynecological Endocrinology Laboratory (MCGEL), Department of Clinical and Experimental Medicine, University of Pisa, Pisa, 56100, Italy; Institute of Medicine and Experimental Biology of Cuyo (IMBECU), CCT-CONICET Mendoza, National University of Cuyo, Parque General San Martin s/n, Mendoza, CP:5500, Argentina.
authors: Angel Matias
authors: AM
authors: Sanchez
authors: Molecular and Cellular Gynecological Endocrinology Laboratory (MCGEL), Department of Clinical and Experimental Medicine, University of Pisa, Pisa, 56100, Italy; Institute of Medicine and Experimental Biology of Cuyo (IMBECU), CCT-CONICET Mendoza, National University of Cuyo, Parque General San Martin s/n, Mendoza, CP:5500, Argentina.
authors: Marina Ines
authors: MI
authors: Flamini
authors: Molecular and Cellular Gynecological Endocrinology Laboratory (MCGEL), Department of Clinical and Experimental Medicine, University of Pisa, Pisa, 56100, Italy.
authors: Eleonora
authors: E
authors: Russo
authors: Molecular and Cellular Gynecological Endocrinology Laboratory (MCGEL), Department of Clinical and Experimental Medicine, University of Pisa, Pisa, 56100, Italy.
authors: Elena
authors: E
authors: Casarosa
authors: Division of Hematology, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, 56100, Italy.
authors: Simone
authors: S
authors: Pacini
authors: Division of Hematology, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, 56100, Italy.
authors: Mario
authors: M
authors: Petrini
authors: Molecular and Cellular Gynecological Endocrinology Laboratory (MCGEL), Department of Clinical and Experimental Medicine, University of Pisa, Pisa, 56100, Italy.
authors: Andrea Riccardo
authors: AR
authors: Genazzani
authors: Molecular and Cellular Gynecological Endocrinology Laboratory (MCGEL), Department of Clinical and Experimental Medicine, University of Pisa, Pisa, 56100, Italy. Electronic address: tommaso.simoncini@med.unipi.it.
authors: Tommaso
authors: T
authors: Simoncini
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D005260:Female; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008840:Microfilament Proteins / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010766:Phosphorylation / Q000187:drug effects; D011962:Receptors, FSH / Q000378:metabolism; D011974:Receptors, LH / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D008840:Microfilament Proteins; D011962:Receptors, FSH; D011974:Receptors, LH; C071058:moesin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D019204:GTP-Binding Proteins
keywords: Breast cancer; FSH; Invasion; LH; Migration