title: Derivation of a valid momentary first-order rate constant for kinetic and energetic analyses of enzymatic reactions.
abstract: To analyze enzymatic reactions energetically for comparison with non-enzymatic reactions (first order) under the same dimension, a method to derive valid momentary first-order rate constants for enzymatic reactions was developed. The momentary first-order rate constant, kenz0= kcat[E'S']e,0/[S]0, was derived for an enzymatic reaction under a certain condition. It was shown that this rate constant is applicable for a wide range of enzymatic reactions. Utilizing this constant, one can conduct reliable kinetic and energetic analyses of enzymatic reactions.
authors: Professor Emeritus, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan imoto1825@beach.ocn.ne.jp.
authors: Taiji
authors: T
authors: Imoto
mesh_terms: D004798:Enzymes / Q000737:chemistry*; D007698:Kinesis; D008956:Models, Chemical
chemical_list: D004798:Enzymes
keywords: energetics; energy barrier; enzyme kinetics; first order; transition state