title: Adrenergic stimulation of diacylglycerol production in genital tract smooth muscle myocytes.
abstract: Diacylglycerol (DAG) production has not been reported in previous studies that have characterized inositol phosphate production during alpha-1 adrenergic receptor signal transduction in the DDT1 MF-2 genital tract myocytes. The current study sought to measure norepinephrine (NE)-stimulated DAG production in these transformed myocytes utilizing thin layer chromatography. DAG production was characterized as an alpha-1 adrenergic mediated event utilizing subtype specific adrenergic agonist and antagonists. DAG production occurred in response to physiologic concentration of NE, was apparent by 30 s and was significantly increased by 2 min. Maximal DAG production was unaffected by pretreatment of the myocytes for 96 h with testosterone, which has previously been shown to induce a doubling of alpha-1 adrenergic receptors in these cells. In contrast, testosterone pretreatment did result in a shift of the dose-response curve resulting in a significantly lower EC50 for NE in the treated cells compared to control myocytes. In conclusion, these studies have confirmed that DAG production occurs as a component of alpha-1 adrenergic signal transduction in the DDT1 MF-2 myocytes; transduction events that were modulated by testosterone resulting in increased agonist sensitivity.
authors: Pritzker School of Medicine, Department of Obstetrics and Gynecology, Maternal Fetal Medicine Section, University of Chicago, 5841 South Maryland, Box MC2050, 60637, Chicago, Illinois.
authors: R J
authors: RJ
authors: Austin
authors: Pritzker School of Medicine, Department of Obstetrics and Gynecology, Maternal Fetal Medicine Section, University of Chicago, 5841 South Maryland, Box MC2050, 60637, Chicago, Illinois.
authors: L
authors: L
authors: DiGiovonni
authors: Pritzker School of Medicine, Department of Obstetrics and Gynecology, Maternal Fetal Medicine Section, University of Chicago, 5841 South Maryland, Box MC2050, 60637, Chicago, Illinois.
authors: M
authors: M
authors: Phillippe
keywords: DDT1 MF-2 myocyte; alpha-1 adrenergic signal transduction; diacylglycerol (DAG); inositol phospholipid pathway; phosphatidylinositol metabolism; testosterone