title: miR-124 inhibits proliferation and invasion of human retinoblastoma cells by targeting STAT3.
abstract: A growing body of evidence suggests that microRNA-124 (miR-124) functions as tumor-suppressor, and involves in tumor initiation, development and metastasis in major classes of human cancers; however, the biological role and underlying molecular mechanism of miR-124 in retinoblastoma (RB) remain unknown. Therefore, we investigated the biological activity and underlying molecular mechanism of miR-124 in human retinoblastoma. In the present study, our results demonstrated the downregulation of miR-124 in RB tissues and RB cell lines compared with normal retinal tissues. The ectopic expression of miR-124 in the RB cell lines (Y79 and SO-RB50) suppresses cell proliferation, migration and invasion, induced cell apoptosis in vitro. Furthermore, signal transducer and activator of transcription 3 (STAT3) was identified as a new target of miR-124, and overexpression of miR-124 decreased STAT3 expression on mRNA level and protein level in human RB cells. We also found that STAT3 mRNA expression was upregulated and inversely correlated with miR-124 expression in the RB tissues (r=-0.683; P<0.001). Restoration of the expression of STAT3 rescues the effects induced by miR-124 in RB cells. The findings of the present study suggested that miR-124 functioned as tumor suppressor in RB, at least in part, by targeting STAT3, and that it could serve as a potential candidate for RB therapeutics.
authors: Department of Ophthalmology, The Second Hospital of Jilin University, Nanguan, Changchun, Jilin 130041, P.R. China.
authors: Shu
authors: S
authors: Liu
authors: Department of Tumor and Hematology, The Second Hospital of Jilin University, Nanguan, Changchun, Jilin 130041, P.R. China.
authors: Chunmei
authors: C
authors: Hu
authors: Department of Electrical Diagnosis, The Second Hospital of Jilin University, Nanguan, Changchun, Jilin 130041, P.R. China.
authors: Yingxue
authors: Y
authors: Wang
authors: Department of Tumor and Hematology, The Second Hospital of Jilin University, Nanguan, Changchun, Jilin 130041, P.R. China.
authors: Guang
authors: G
authors: Shi
authors: Department of Tumor and Hematology, The Second Hospital of Jilin University, Nanguan, Changchun, Jilin 130041, P.R. China.
authors: Yarong
authors: Y
authors: Li
authors: Department of Ophthalmology, The Second Hospital of Jilin University, Nanguan, Changchun, Jilin 130041, P.R. China.
authors: Huang
authors: H
authors: Wu
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D012160:Retina / Q000378:metabolism; D012175:Retinoblastoma / Q000235:genetics* / Q000473:pathology; D050796:STAT3 Transcription Factor / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C509207:MIRN124 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human