title: Stabilization of starch-based microgel-lysozyme complexes using a layer-by-layer assembly technique.
abstract: The layer-by-layer assembly of polyelectrolyte multilayers of chitosan (CS) and carboxymethyl starch (CMS) on soft and porous pH- and ionic strength-response microgels was determined by confocal laser scanning microscopy (CLSM) and zeta potential measurements. In vitro release of lysozyme from the stabilized microgels under simulated gastric and intestinal fluids was also investigated. The distribution of CS in the microgels was identified by CLSM, and the optimal molecular weight of CS was 100kDa, which could only be absorbed on the microgel surface. The CS was used as the first layer, while the CMS was used as the second layer, and the zeta potential revealed that the optimal weight ratios of CS and CMS to microgels in the complexes were 0.1 and 0.06, respectively. The in vitro release experiments suggested that the stabilized double-layer microgel complexes could potentially be applied as a carrier system to prevent early release in the stomach to target intestinal delivery.
authors: School of Food Science and Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
authors: Bao
authors: B
authors: Zhang
authors: School of Food Science and Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
authors: Yi
authors: Y
authors: Pan
authors: School of Food Science and Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
authors: Hanqing
authors: H
authors: Chen
authors: School of Food Science and Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
authors: Tengfei
authors: T
authors: Liu
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.
authors: Han
authors: H
authors: Tao
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China. Electronic address: yqtian@jiangnan.edu.cn.
authors: Yaoqi
authors: Y
authors: Tian
mesh_terms: D048271:Chitosan / Q000378:metabolism*; D005782:Gels; D018613:Microscopy, Confocal; D009113:Muramidase / Q000378:metabolism*; D009994:Osmolar Concentration; D013213:Starch / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D005782:Gels; D013213:Starch; D048271:Chitosan; C034848:carboxymethyl starch; D009113:Muramidase
keywords: CMS; Chitosan; Layer-by-layer assembly; Starch-based microgels