title: Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells.
abstract: Myotonic dystrophy 1 (DM1) is a common form of muscular dystrophy. Although several animal models have been established for DM1, myoblast cell models are still important because they offer an efficient cellular alternative for studying cellular and molecular events. Though C2C12 myoblast cells have been widely used to study myogenesis, resistance to gene transfection, or viral transduction, hinders research in C2C12 cells. Here, we describe an optimized protocol that includes daily maintenance, transfection and transduction procedures to introduce genes into C2C12 myoblasts and the induction of myocyte differentiation. Collectively, these procedures enable best transfection/transduction efficiencies, as well as consistent differentiation outcomes. The protocol described in establishing DM1 myoblast cell models would benefit the study of myotonic dystrophy, as well as other muscular diseases.
authors: Department of Biology and Biochemistry, University of Houston.
authors: Rui
authors: R
authors: Liang
authors: Department of Biology and Biochemistry, University of Houston; Department of Cardiology, The First Affiliated Hospital of Nanchang University.
authors: Wei
authors: W
authors: Dong
authors: Department of Biology and Biochemistry, University of Houston.
authors: Xiaopeng
authors: X
authors: Shen
authors: Department of Biology and Biochemistry, University of Houston; Department of Cardiology, The First Affiliated Hospital of Nanchang University.
authors: Xiaoping
authors: X
authors: Peng
authors: Department of Biology and Biochemistry, University of Houston; Department of Economics, University of Houston.
authors: Angie G
authors: AG
authors: Aceves
authors: Department of Biology and Biochemistry, University of Houston; yliu51@central.uh.edu.
authors: Yu
authors: Y
authors: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D051379:Mice; D032446:Myoblasts; D009223:Myotonic Dystrophy; D014162:Transfection