title: Superhydrophilic cotton thread with temperature-dependent pattern for sensitive nucleic acid detection.
abstract: Cotton thread is promising in fabricating biosensors for diagnostic application due to its excellent characteristics. However, the enrichment of the capture molecules on a narrow zone of the cotton thread based biosensor is a big challenge because of its superhydrophilicity. Here, we report a simple, low-cost and accurate cotton thread based nucleic acid biosensor with temperature-dependent pattern. Liquid wax is used to fabricate temperature-dependent pattern to restrict the test zone in a narrow area. This biosensor enables visual and quantitative detection of target DNA by accumulation of gold nanoparticles (GNPs) on the test zone with a detection limits of 0.75nM. In addition, the cotton thread based biosensor needs less sample than previous reported lateral flow strip and the sample solution wicks faster at the cotton thread which can lead to a shorter detection time. This simple, low-cost and fast detection method holds great potential to improve healthcare services in the developing regions.
authors: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science & Technology Beijing, Beijing 100083, PR China.
authors: Tingting
authors: T
authors: Wu
authors: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science & Technology Beijing, Beijing 100083, PR China.
authors: Tailin
authors: T
authors: Xu
authors: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science & Technology Beijing, Beijing 100083, PR China.
authors: Li-Ping
authors: LP
authors: Xu
authors: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science & Technology Beijing, Beijing 100083, PR China.
authors: Yan
authors: Y
authors: Huang
authors: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science & Technology Beijing, Beijing 100083, PR China.
authors: Wanxin
authors: W
authors: Shi
authors: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science & Technology Beijing, Beijing 100083, PR China.
authors: Yongqiang
authors: Y
authors: Wen
authors: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science & Technology Beijing, Beijing 100083, PR China.
authors: Xueji
authors: X
authors: Zhang
mesh_terms: D065966:Absorption, Physicochemical; D003124:Colorimetry / Q000295:instrumentation*; D032561:Cotton Fiber; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D053768:Metal Nanoparticles / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D000067716:Point-of-Care Testing; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D013817:Thermography / Q000295:instrumentation*
chemical_list: D006046:Gold; D004247:DNA
keywords: Cotton thread; Enrichment; Nucleic acid; Temperature-dependent pattern; Visual detection