title: Highly sensitive and selective detection of Al(III) ions in aqueous buffered solution with fluorescent peptide-based sensor.
abstract: A fluorescent sensor based on a tripeptide (SerGluGlu) with a dansyl fluorophore detected selectively Al(III) among 16 metal ions in aqueous buffered solutions without any organic cosolvent. The peptide-based sensor showed a highly sensitive turn on response to aluminium ion with high binding affinity (1.84×10(4)M(-1)) in aqueous buffered solutions. The detection limit (230nM, 5.98ppb) of the peptide-based sensor was much lower than the maximum allowable level (7.41μM) of aluminium ions in drinking water demanded by EPA. The binding mode of the peptide sensor with aluminium ions was characterized using ESI mass spectrometry, NMR titration, and pH titration experiments.
authors: Bioorganic Chemistry Lab, Center for Design and Applications of Molecular Catalysts, Department of Chemistry and Chemical Engineering, Inha University, Incheon 402-751, South Korea.
authors: Byunggyu
authors: B
authors: In
authors: Bioorganic Chemistry Lab, Center for Design and Applications of Molecular Catalysts, Department of Chemistry and Chemical Engineering, Inha University, Incheon 402-751, South Korea.
authors: Gi Won
authors: GW
authors: Hwang
authors: Bioorganic Chemistry Lab, Center for Design and Applications of Molecular Catalysts, Department of Chemistry and Chemical Engineering, Inha University, Incheon 402-751, South Korea. Electronic address: leekh@inha.ac.kr.
authors: Keun-Hyeung
authors: KH
authors: Lee
mesh_terms: D000535:Aluminum / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D014867:Water / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D012996:Solutions; D014867:Water; D000535:Aluminum
keywords: Al(III); Chemosensor; Fluorescence; Peptide; Sensor; Turn-on