title: Transcriptional response of mysid crustacean, Americamysis bahia, is affected by subchronic exposure to nonylphenol.
abstract: Nonylphenol (NP) has been classified as an endocrine-disrupting chemical. In this study, we conducted mysid DNA microarray analysis with which has 2240 oligo DNA probes to observe differential gene expressions in mysid crustacean (Americamysis bahia) exposed to 1, 3, 10 and 30 μg/l of NP for 14 days. As a result, we found 31, 27, 39 and 68 genes were differentially expressed in the respective concentrations. Among these genes, the expressions of five particular genes were regulated in a similar manner at all concentrations of the NP exposure. So, we focused on one gene encoding cuticle protein, and another encoding cuticular protein analogous to peritrophins 1-H precursor. These genes were down-regulated by NP exposure in a dose-dependent manner, and it suggested that they were related in a reduction of the number of molting in mysids. Thus, they might become useful molecular biomarker candidates to evaluate molting inhibition in mysids.
authors: Mizuki biotech, Co., Ltd., 1-1 Hyakunenkouen, Kurume, Fukuoka 839-0864, Japan.
authors: Masaya
authors: M
authors: Uchida
authors: Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan; Department of Biological and Chemical Systems Engineering, National Institute of Technology, Kumamoto College, 2627 Hirayama-shinmachi, Yatsushiro, Kumamoto 866-8501, Japan.
authors: Masashi
authors: M
authors: Hirano
authors: Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
authors: Hiroshi
authors: H
authors: Ishibashi
authors: Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Higashi-ku, Tsukide, Kumamoto 862-8502, Japan.
authors: Jun
authors: J
authors: Kobayashi
authors: Mizuki biotech, Co., Ltd., 1-1 Hyakunenkouen, Kurume, Fukuoka 839-0864, Japan.
authors: Yoshihiro
authors: Y
authors: Kagami
authors: Mizuki biotech, Co., Ltd., 1-1 Hyakunenkouen, Kurume, Fukuoka 839-0864, Japan.
authors: Akiko
authors: A
authors: Koyanagi
authors: Mizuki biotech, Co., Ltd., 1-1 Hyakunenkouen, Kurume, Fukuoka 839-0864, Japan.
authors: Teruhiko
authors: T
authors: Kusano
authors: Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Higashi-ku, Tsukide, Kumamoto 862-8502, Japan.
authors: Minoru
authors: M
authors: Koga
authors: Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Higashi-ku, Tsukide, Kumamoto 862-8502, Japan. Electronic address: arizono@pu-kumamoto.ac.jp.
authors: Koji
authors: K
authors: Arizono
mesh_terms: D000818:Animals; D003445:Crustacea / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015536:Down-Regulation; D052244:Endocrine Disruptors / Q000633:toxicity*; D004781:Environmental Exposure / Q000032:analysis*; D004785:Environmental Pollutants / Q000633:toxicity*; D018978:Molting / Q000187:drug effects* / Q000235:genetics; D010636:Phenols / Q000633:toxicity*; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D010636:Phenols; C025256:nonylphenol
keywords: DNA microarray; Mysid (Americamysis bahia); Nonylphenol; Transcriptome