title: Replacement of Glycoprotein B in Alcelaphine Herpesvirus 1 by Its Ovine Herpesvirus 2 Homolog : Implications in Vaccine Development for Sheep-Associated Malignant Catarrhal Fever.
abstract: Vaccine development is a top priority in malignant catarrhal fever (MCF) research. In the case of sheep-associated MCF (SA-MCF) caused by ovine herpesvirus 2 (OvHV-2), progress toward this objective has been hindered by the absence of methods to attenuate or modify the virus, since it cannot be propagated in vitro. As an alternative for vaccine development, in this study, we tested the hypothesis that one of the SA-MCF vaccine candidate targets, OvHV-2 glycoprotein B (gB), could be expressed by a nonpathogenic alcelaphine herpesvirus 1 (AlHV-1) and then evaluated the potential of the AlHV-1/OvHV-2 chimera to be used as a vaccine and a diagnostic tool. The construction and characterization of an AlHV-1/OvHV-2 chimeric virus that is nonpathogenic and expresses an OvHV-2 vaccine target are significant steps toward the development of an SA-MCF vaccine and also provide a valuable means to study OvHV-2 biology.
authors: Animal Disease Research Unit, Agricultural Research Service, USDA, Pullman, Washington, USA; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.
authors: Cristina W
authors: 0000-0002-8168-5448
authors: CW
authors: Cunha
authors: Animal Disease Research Unit, Agricultural Research Service, USDA, Pullman, Washington, USA; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.
authors: Naomi S
authors: NS
authors: Taus
authors: Fundamental and Applied Research in Animals and Health (FARAH), Immunology-Vaccinology, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors: Benjamin G
authors: BG
authors: Dewals
authors: Fundamental and Applied Research in Animals and Health (FARAH), Immunology-Vaccinology, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors: Alain
authors: A
authors: Vanderplasschen
authors: Animal Disease Research Unit, Agricultural Research Service, USDA, Pullman, Washington, USA; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.
authors: Donald P
authors: DP
authors: Knowles
authors: Animal Disease Research Unit, Agricultural Research Service, USDA, Pullman, Washington, USA; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.
authors: Hong
authors: H
authors: Li
keywords: Chimeric virus; alcelaphine herpesvirus 1; malignant catarrhal fever; ovine herpesvirus 2; vaccine