title: p-Hydroxylcinnamaldehyde induces the differentiation of oesophageal carcinoma cells via the cAMP-RhoA-MAPK signalling pathway.
abstract: p-Hydroxylcinnamaldehyde (CMSP) has been identified as an inhibitor of the growth of various cancer cells. However, its function in oesophageal squamous cell carcinoma (ESCC) and the underlying mechanism remain unclear. The aim of the present study was to characterize the differentiation effects of CMSP, as well as its mechanism in the differentiation of ESCC Kyse30 and TE-13 cells. The function of CMSP in the viability, colony formation, migration and invasion of Kyse30 and TE-13 cells was determined by MTS, colony-formation, wound healing and transwell assays. Western blotting and pull-down assays were used to investigate the effect of CMSP on the expression level of malignant markers of ESCC, as well as the activity of MAPKs, RhoA and GTP-RhoA in Kyse30 and TE-13 cells. We found that CMSP could inhibit proliferation and migration and induce Kyse30 and TE-13 cell differentiation, characterized by dendrite-like outgrowth, decreased expression of tumour-associated antigens, as well as the decreased expression of malignant markers. Furthermore, increased cAMP, p-P38 and decreased activities of ERK, JNK and GTP-RhoA, were detected after treatment with CMSP. These results indicated that CMSP induced the differentiation of Kyse30 and TE-13 cells through mediating the cAMP-RhoA-MAPK axis, which might provide new potential strategies for ESCC treatment.
authors: Clinical Laboratory, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P. R. China.|Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P. R. China.
authors: Ming
authors: M
authors: Ma
authors: Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P. R. China.
authors: Lian-Mei
authors: LM
authors: Zhao
authors: Department of Infection Management, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P. R. China.
authors: Xing-Xiao
authors: XX
authors: Yang
authors: Department of Infection Management, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P. R. China.
authors: Ya-Nan
authors: YN
authors: Shan
authors: Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P. R. China.
authors: Wen-Xuan
authors: WX
authors: Cui
authors: Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P. R. China.
authors: Liang
authors: L
authors: Chen
authors: Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P. R. China.
authors: Bao-En
authors: BE
authors: Shan
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002934:Cinnamates / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000378:metabolism*; D000077277:Esophageal Squamous Cell Carcinoma; D004947:Esophagus / Q000378:metabolism; D006801:Humans; D020935:MAP Kinase Signaling System; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D002934:Cinnamates; C117045:p-hydroxycinnamaldehyde; C066737:RHOA protein, human; D000171:Acrolein; D000242:Cyclic AMP; D020742:rhoA GTP-Binding Protein; C012843:cinnamaldehyde