title: Contrasting silver nanoparticle toxicity and detoxification strategies in Microcystis aeruginosa and Chlorella vulgaris: New insights from proteomic and physiological analyses.
abstract: Several studies have shown that AgNPs can be toxic to phytoplankton, but the underlying cellular mechanisms still remain largely unknown. Here we studied the toxicity and detoxification of AgNPs (and ionic silver released by the AgNPs) in a prokaryotic (Microcystis aeruginosa) and a eukaryotic (Chlorella vulgaris) freshwater phytoplankton species using a combination of proteomic, gene transcription, and physiological analyses. We show that AgNPs were more toxic to the growth, photosynthesis, antioxidant systems, and carbohydrate metabolism of M. aeruginosa than of C. vulgaris. C. vulgaris could detoxify efficiently AgNPs-induced ROS species via induction of antioxidant enzymes (superoxide dismutase or SOD, peroxidase or POD, catalase or CAT, and glutamine synthetase), allowing photosynthesis to continue unabated at growth-inhibitory AgNPs concentration. By contrast, the transcription and expression of SOD and POD in M. aeruginosa was inhibited by the same AgNPs exposure. The present study shed new lights on the AgNPs toxicity mechanisms and detoxification strategies in two freshwater algae of contrasting AgNPs sensitivity.
authors: College of Environment, Zhejiang University of Technology, Hangzhou 310032, PR China.
authors: Haifeng
authors: H
authors: Qian
authors: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, PR China.
authors: Kun
authors: K
authors: Zhu
authors: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.
authors: Haiping
authors: H
authors: Lu
authors: Quebec-Ocean and Takuvik Joint International Research Unit, Université Laval, Québec, Canada.
authors: Michel
authors: M
authors: Lavoie
authors: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310032, PR China.
authors: Si
authors: S
authors: Chen
authors: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors: Zhongjing
authors: Z
authors: Zhou
authors: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors: Zhiping
authors: Z
authors: Deng
authors: College of Environment, Zhejiang University of Technology, Hangzhou 310032, PR China.
authors: Jun
authors: J
authors: Chen
authors: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, PR China. Electronic address: azwfu@zjut.edu.cn.
authors: Zhengwei
authors: Z
authors: Fu
mesh_terms: D020418:Algal Proteins / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D048408:Chlorella vulgaris / Q000502:physiology*; D008658:Inactivation, Metabolic; D053768:Metal Nanoparticles / Q000633:toxicity*; D046931:Microcystis / Q000502:physiology*; D020543:Proteome; D012834:Silver / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D020418:Algal Proteins; D001426:Bacterial Proteins; D020543:Proteome; D014874:Water Pollutants, Chemical; D012834:Silver
keywords: Antioxidant; Chlorella vulgaris; DIGE; Microcystis aeruginosa; Silver nanoparticles; Silver toxicity