title: Enrofloxacin and Probiotic Lactobacilli Influence PepT1 and LEAP-2 mRNA Expression in Poultry.
abstract: Expression of peptide transporter 1 (PepT1) and liver-expressed antimicrobial peptide 2 (LEAP-2) in chickens can be influenced by food deprivation, pathological conditions and drug administration. Effect of three putative probiotic Lactobacillus strains and enrofloxacin on the expression of PepT1 and LEAP-2 mRNA was investigated in Ross 308 chickens. One-day-old chicks (n = 24) were allocated to following groups: control (without treatment); group treated with probiotics via feed; group treated with a combination of probiotics and enrofloxacin; and a group given enrofloxacin only. The drug was administered at a dose of 10 mg kg-1, via drinking water for 5 days. Samples from liver, duodenum and jejunum were collected 126 h after the start of the treatment. Expression levels of PepT1 and LEAP-2 were determined by real-time polymerase chain reaction and were statistically evaluated by Mann-Whitney test. Enrofloxacin administered alone or in combination with probiotics provoked a statistically significant up-regulation of PepT1 mRNA levels in the measured organ sites. These changes can be attributed to a tendency of improvement in utilization of dietary peptide and in body weight gain. LEAP-2 mRNA expression levels did not change significantly in enrofloxacin-treated chickens in comparison with control group.
authors: Department of Pharmacology, Veterinary Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria.
authors: Ivelina
authors: I
authors: Pavlova
authors: Department of Pharmacology, Veterinary Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria. akmilanova@gmail.com.
authors: Aneliya
authors: A
authors: Milanova
authors: The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences (BAS), 26, Akad. G. Bontchev, str., Sofia, Bulgaria.
authors: Svetla
authors: S
authors: Danova
authors: Division of Veterinary Pharmacology, Pharmacotherapy and Toxicology, Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors: Johanna
authors: J
authors: Fink-Gremmels
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D002645:Chickens / Q000235:genetics / Q000382:microbiology; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000502:physiology*; D007778:Lactobacillus; D000074011:Peptide Transporter 1 / Q000235:genetics*; D019936:Probiotics; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D023181:Antimicrobial Cationic Peptides; D024841:Fluoroquinolones; D000074011:Peptide Transporter 1; D012333:RNA, Messenger; D000077422:Enrofloxacin
keywords: Chicken; Enrofloxacin; LEAP-2 mRNA; PepT1 mRNA; Probiotics