title: STED and STORM Superresolution Imaging of Primary Cilia.
abstract: The characteristic lengths of molecular arrangement in primary cilia are below the diffraction limit of light, challenging structural and functional studies of ciliary proteins. Superresolution microscopy can reach up to a 20 nm resolution, significantly improving the ability to map molecules in primary cilia. Here we describe detailed experimental procedure of STED microscopy imaging and dSTORM imaging, two of the most powerful superresolution imaging techniques. Specifically, we emphasize the use of these two methods on imaging proteins in primary cilia.
authors: Institute of Atomic and Molecular Sciences, Academia Sinica, No 1, Roosevelt Rd. Sec 4, Taipei, 10617, Taiwan.
authors: T Tony
authors: TT
authors: Yang
authors: Institute of Atomic and Molecular Sciences, Academia Sinica, No 1, Roosevelt Rd. Sec 4, Taipei, 10617, Taiwan.
authors: Weng Man
authors: WM
authors: Chong
authors: Institute of Atomic and Molecular Sciences, Academia Sinica, No 1, Roosevelt Rd. Sec 4, Taipei, 10617, Taiwan. jcliao@iams.sinica.edu.tw.
authors: Jung-Chi
authors: JC
authors: Liao
mesh_terms: D002923:Cilia / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000378:metabolism
keywords: Ciliary protein; Diffraction limit; Fluorophore; Primary cilium; STED; STORM; Superresolution microscopy