title: DJ-1/Park7 Sensitive Na+/H+Exchanger 1 (NHE1) in CD4+T Cells.
abstract: DJ-1/Park7 is a redox-sensitive chaperone protein counteracting oxidation and presumably contributing to the control of oxidative stress responses and thus inflammation. DJ-1 gene deletion exacerbates the progression of Parkinson's disease presumably by augmenting oxidative stress. Formation of reactive oxygen species (ROS) is paralleled by activation of the Na+/H+exchanger 1 (NHE1). ROS formation in CD4+T cells plays a decisive role in regulating inflammatory responses. In the present study, we explored whether DJ-1 is expressed in CD4+T cells, and affects ROS production as well as NHE1 in those cells. To this end, DJ-1 and NHE1 transcript, and protein levels were quantified by qRT-PCR and Western blotting, respectively, intracellular pH (pHi) utilizing bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence, NHE activity from realkalinization after an ammonium pulse, and ROS production utilizing 2',7' -dichlorofluorescin diacetate (DCFDA) fluorescence. As a result DJ-1 was expressed in CD4+T cells. ROS formation, NHE1 transcript levels, NHE1 protein, and NHE activity were higher in CD4+T cells from DJ-1 deficient mice than in CD4+T cells from wild type mice. Antioxidant N-acetyl-cysteine (NAC) and protein tyrosine kinase (PTK) inhibitor staurosporine decreased the NHE activity in DJ-1 deficient CD4+T cells, and blunted the difference between DJ-1-/-and DJ-1+/+CD4+T cells, an observation pointing to a role of ROS in the up-regulation of NHE1 in DJ-1-/-CD4+T cells. In conclusion, DJ-1 is a powerful regulator of ROS production as well as NHE1 expression and activity in CD4+T cells. J. Cell. Physiol. 232: 3050-3059, 2017. © 2016 Wiley Periodicals, Inc.
authors: Department of Cardiology, Vascular Medicine and Physiology, Eberhard-Karls-University, Tübingen, Germany.
authors: Yuetao
authors: Y
authors: Zhou
authors: Department of Cardiology, Vascular Medicine and Physiology, Eberhard-Karls-University, Tübingen, Germany.
authors: Xiaolong
authors: X
authors: Shi
authors: Department of Cardiology, Vascular Medicine and Physiology, Eberhard-Karls-University, Tübingen, Germany.
authors: Hong
authors: H
authors: Chen
authors: Department of Cardiology, Vascular Medicine and Physiology, Eberhard-Karls-University, Tübingen, Germany.|Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, China.
authors: Shaqiu
authors: S
authors: Zhang
authors: Department of Cardiology, Vascular Medicine and Physiology, Eberhard-Karls-University, Tübingen, Germany.
authors: Madhuri S
authors: MS
authors: Salker
authors: Institute of Clinical Anatomy and Cell Analysis, Eberhard-Karls-University, Tübingen, Germany.
authors: Andreas F
authors: AF
authors: Mack
authors: Department of Cardiology, Vascular Medicine and Physiology, Eberhard-Karls-University, Tübingen, Germany.|Campbell Family Institute for Breast Cancer Research, Ontario Cancer Institute, UHN, Toronto, Canada.|Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
authors: Michael
authors: M
authors: Föller
authors: Campbell Family Institute for Breast Cancer Research, Ontario Cancer Institute, UHN, Toronto, Canada.
authors: Tak W
authors: TW
authors: Mak
authors: Department of Cardiology, Vascular Medicine and Physiology, Eberhard-Karls-University, Tübingen, Germany.
authors: Yogesh
authors: Y
authors: Singh
authors: Department of Cardiology, Vascular Medicine and Physiology, Eberhard-Karls-University, Tübingen, Germany.
authors: Florian
authors: F
authors: Lang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005838:Genotype; D006863:Hydrogen-Ion Concentration; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D000071617:Protein Deglycase DJ-1 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D027682:Cation Transport Proteins; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C490997:Slc9a1 protein, mouse; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers; D011505:Protein-Tyrosine Kinases; C503466:PARK7 protein, mouse; D000071617:Protein Deglycase DJ-1