title: Degradation of acephate by Enterobacter asburiae, Bacillus cereus and Pantoea agglomerans isolated from diamondback moth Plutella xylostella (L), a pest of cruciferous crops.
abstract: Acephate-degrading bacterial isolates were isolated from the larval gut of diamondback moth Plutella xylostella, a notorious pest of cruciferous crops worldwide that has developed resistance to insecticides. Partial 16S rRNA gene sequencing identified the isolates as Bacillus cereus (PX-B.C.Or), Enterobacter asburiae (PXE), and Pantoae agglomerans (PX-Pt.ag.Jor). All isolates grew on minimal media (MM) in the presence of acephate at 100 and 200 ppm, with maximum growth at 200 ppm. LC-MS analyses of spent medium showed that E. asburiae degraded acephate to methamidophos and O, O-dimethyl phosporamidate and B. cereus O,S-dimethyl to phosphorothioate but P. agglomerans to an unnamed compound. All three isolates used acephate as a source of carbon and energy for growth; however, P. agglomerans used it also as source of sulphur. Strong evidence revealed that the bacterial communities present in the gut of diamondback moth might aid in acephate degradation and play a role in the development of insecticide resistance.
authors: Shanivarsanthe Leelesh
authors: SL
authors: Ramya
authors: Thiruvengadam
authors: T
authors: Venkatesan
authors: Kottilingam Srinivasa
authors: KS
authors: Murthy
authors: Sushil Kumar
authors: SK
authors: Jalali
authors: Abraham
authors: A
authors: Varghese
mesh_terms: D000818:Animals; D001409:Bacillus cereus / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019607:Brassicaceae / Q000254:growth & development; D018556:Crops, Agricultural / Q000254:growth & development; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004754:Enterobacter / Q000235:genetics / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D007814:Larva / Q000254:growth & development / Q000382:microbiology; D008969:Molecular Sequence Data; D009036:Moths / Q000254:growth & development / Q000382:microbiology*; D009946:Organothiophosphorus Compounds / Q000378:metabolism*; D020636:Pantoea / Q000235:genetics / Q000378:metabolism*; D063088:Phosphoramides / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D063088:Phosphoramides; D012336:RNA, Ribosomal, 16S; C001969:acephate