title: The Eucalyptus linker histone variant EgH1.3 cooperates with the transcription factor EgMYB1 to control lignin biosynthesis during wood formation.
abstract: Wood, also called secondary xylem, is a specialized vascular tissue constituted by different cell types that undergo a differentiation process involving deposition of thick, lignified secondary cell walls. The mechanisms needed to control the extent of lignin deposition depending on the cell type and the differentiation stage are far from being fully understood. We found that the Eucalyptus transcription factor EgMYB1, which is known to repress lignin biosynthesis, interacts specifically with a linker histone variant, EgH1.3. This interaction enhances the repression of EgMYB1's target genes, strongly limiting the amount of lignin deposited in xylem cell walls. The expression profiles of EgMYB1 and EgH1.3 overlap in xylem cells at early stages of their differentiation as well as in mature parenchymatous xylem cells, which have no or only thin lignified secondary cell walls. This suggests that a complex between EgMYB1 and EgH1.3 integrates developmental signals to prevent premature or inappropriate lignification of secondary cell walls, providing a mechanism to fine-tune the differentiation of xylem cells in time and space. We also demonstrate a role for a linker histone variant in the regulation of a specific developmental process through interaction with a transcription factor, illustrating that plant linker histones have other functions beyond chromatin organization.
authors: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 31326, Castanet-Tolosan, France.
authors: Marçal
authors: M
authors: Soler
authors: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 31326, Castanet-Tolosan, France.
authors: Anna
authors: A
authors: Plasencia
authors: UMR1121 'Agronomie et Environnement' Nancy-Colmar, Institute Nationale de la Recherche Agronomique (INRA), TSA 40602, 54518, Vandoeuvre Cedex, France.|UMR1121 'Agronomie et Environnement' Nancy-Colmar, Université de Lorraine, TSA 40602, 54518, Vandoeuvre Cedex, France.
authors: Romain
authors: R
authors: Larbat
authors: Fédération de Recherche 3450, Plateforme Imagerie, 31326, Castanet-Tolosan, France.
authors: Cécile
authors: C
authors: Pouzet
authors: Fédération de Recherche 3450, Plateforme Imagerie, 31326, Castanet-Tolosan, France.
authors: Alain
authors: A
authors: Jauneau
authors: Laboratoire des Interactions Plantes-Microorganismes (LIPM), Université de Toulouse, INRA, CNRS, 31326, Castanet-Tolosan, France.
authors: Susana
authors: S
authors: Rivas
authors: Department of Plant Physiology, Umeå University, SE-901 87, Umeå, Sweden.
authors: Edouard
authors: E
authors: Pesquet
authors: Jean-Pierre Bourgin Institute, INRA/AgroParisTech, UMR1318, Saclay Plant Science, 78026, Versailles, France.
authors: Catherine
authors: C
authors: Lapierre
authors: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 31326, Castanet-Tolosan, France.
authors: Isabelle
authors: I
authors: Truchet
authors: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 31326, Castanet-Tolosan, France.
authors: Jacqueline
authors: J
authors: Grima-Pettenati
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D005052:Eucalyptus / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D006657:Histones / Q000378:metabolism*; D008031:Lignin / Q000096:biosynthesis*; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics; D014934:Wood / Q000378:metabolism*; D052584:Xylem / Q000254:growth & development / Q000378:metabolism
chemical_list: D006657:Histones; D010940:Plant Proteins; D014157:Transcription Factors; D008031:Lignin
keywords: MYB transcription factors; cambium; lignin; linker histones; protein-protein interactions; secondary cell wall; wood formation; xylem