title: Stability and localization of 14-3-3 proteins are involved in salt tolerance in Arabidopsis.
abstract: Salt stress induces the degradation of 14-3-3 proteins, and affects the localization of 14-3-3 λ. Both the modulation of 14-3-3 protein stability and the subcellular localization of these proteins are involved in salt tolerance in plants. Salt tolerance in plants is regulated by multiple signaling pathways, including the salt overly sensitive (SOS) pathway, of which the SOS2 protein is a key component. SOS2 is activated under salt stress to enhance salt tolerance in plants. We previously identified 14-3-3 λ and κ as important regulators of salt tolerance. Both proteins interact with SOS2 to inhibit its kinase activity under normal growth conditions. In response to salt stress, 14-3-3 proteins dissociate from SOS2, releasing its activity and activating the SOS pathway to confer salt tolerance (Zhou et al. Plant Cell 26:1166-1182, 2014). Here we report that salt stress promotes the degradation of 14-3-3 λ and κ, at least in part via the actions of SOS3-like calcium binding protein 8/calcineurin-B-like10, and also decreases the plasma membrane (PM) localization of 14-3-3 λ. Salt stress also partially represses the interaction of SOS2 and 14-3-3 λ at the PM, but activates PM-localized SOS2. Together, these results suggest that, in plants, both the modulation of 14-3-3 stability and the subcellular localization of these proteins in response to salt stress are important for SOS2 activation and salt tolerance. These data provide new insights into the biological roles of 14-3-3 proteins in modulating salt tolerance.
authors: College of Life Sciences, Sichuan University, Chengdu, 610064, China.|Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, Sichuan University, Chengdu, 610064, China.
authors: Tinghong
authors: T
authors: Tan
authors: College of Life Sciences, Sichuan University, Chengdu, 610064, China.|Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, Sichuan University, Chengdu, 610064, China.
authors: Jingqing
authors: J
authors: Cai
authors: College of Life Sciences, Sichuan University, Chengdu, 610064, China.|Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, Sichuan University, Chengdu, 610064, China.
authors: Erbao
authors: E
authors: Zhan
authors: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors: Yongqing
authors: Y
authors: Yang
authors: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors: Jinfeng
authors: J
authors: Zhao
authors: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors: Yan
authors: Y
authors: Guo
authors: College of Life Sciences, Sichuan University, Chengdu, 610064, China. zhouhuapeng2013@gmail.com.|Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, Sichuan University, Chengdu, 610064, China. zhouhuapeng2013@gmail.com.
authors: Huapeng
authors: H
authors: Zhou
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics; D055049:Salt Tolerance / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D054875:Ubiquitination
chemical_list: D048948:14-3-3 Proteins; D029681:Arabidopsis Proteins; D012965:Sodium Chloride
keywords: 14-3-3 proteins; SOS pathway; Salt tolerance; Stability; Subcellular localization; Ubiquitination