title: A fast and environment-friendly MEPSPEP/UHPLC-PDA methodology to assess 3-hydroxy-4,5-dimethyl-2(5H)-furanone in fortified wines.
abstract: Sotolon is widely associated with the quality of fortified aged wines, and has also been linked to premature oxidative aging (premox). Here we developed a single, fast and environmental-friendly microextraction by packed sorbent ultra-high pressure liquid chromatography analysis (MEPS/UHPLC-PDA) for sotolon quantification in different wines. The best extraction conditions (loading three times 250μL samples through the MEPSPEP sorbent and elution with 100μL of 50% MeOH) were combined with a fast UHPLC separation (5min separation using acidified 10% MeOH isocratic flow in a CORTECS C18 column) to allow unparalleled minimum sample and solvents volumes usage. The validated methodology showed good linearity (r(2)>0.993) and precision (<5.6%); high recovery (>81%) and detection limits (0.45-2.51μg/L) far below sotolon odor threshold for any type of wine. The methodology was successfully applied to selected white table and Madeira wines, encompassing therefore a wide range of alcohol and sugar contents. Furthermore, as far we may know, this is the first time a single methodology can be used to assess both wine aging or premox according to the type of wine.
authors: CQM - Centro de Química da Madeira, NPRG, Campus Universitário da Penteada, 9000-390 Funchal, Portugal.
authors: Jorge
authors: J
authors: Freitas
authors: CQM - Centro de Química da Madeira, NPRG, Campus Universitário da Penteada, 9000-390 Funchal, Portugal.
authors: Rosa
authors: R
authors: Perestrelo
authors: CQM - Centro de Química da Madeira, NPRG, Campus Universitário da Penteada, 9000-390 Funchal, Portugal.
authors: Rubina
authors: R
authors: Cassaca
authors: CQM - Centro de Química da Madeira, NPRG, Campus Universitário da Penteada, 9000-390 Funchal, Portugal.
authors: Mariangie
authors: M
authors: Castillo
authors: CQM - Centro de Química da Madeira, NPRG, Campus Universitário da Penteada, 9000-390 Funchal, Portugal.
authors: Mariana
authors: M
authors: Santos
authors: CQM - Centro de Química da Madeira, NPRG, Campus Universitário da Penteada, 9000-390 Funchal, Portugal.
authors: Jorge
authors: J
authors: Pereira
authors: CQM - Centro de Química da Madeira, NPRG, Campus Universitário da Penteada, 9000-390 Funchal, Portugal; Faculdade de Ciências Exatas e da Engenharia da Universidade da Madeira, Campus Universitário da Penteada, 9000-390 Funchal, Portugal. Electronic address: jsc@uma.pt.
authors: José S
authors: JS
authors: Câmara
mesh_terms: D000438:Alcohols / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D057230:Limit of Detection; D016014:Linear Models; D009930:Organic Chemicals / Q000737:chemistry; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction; D012997:Solvents / Q000737:chemistry; D014920:Wine / Q000032:analysis*
chemical_list: D000438:Alcohols; D009930:Organic Chemicals; D012997:Solvents
keywords: Microextraction by packed sorbent (MEPS); Oxidative aging; Premox; Sotolon; Wine