title: Osmotic stress adaptation of Paracoccidioides lutzii, Pb01, monitored by proteomics.
abstract: The ability to respond to stressful conditions is essential for most living organisms. In pathogenic organisms, this response is required for effective transition from a saprophytic lifestyle to the establishment of pathogenic interactions within a susceptible host. Hyperosmotic stress has been used as a model to study signal transduction and seems to cause many cellular adaptations, including the alteration of protein expression and cellular volume as well as size regulation. In this work, we evaluated the proteomic profile of Paracoccidioides lutzii Pb01 yeast cells during osmotic stress induced by potassium chloride. We performed a high accuracy proteomic technique (NanoUPLC-MS(E)) to identify differentially expressed proteins during osmotic shock. The data describe an osmoadaptative response of this fungus when subjected to this treatment. Proteins involved in the synthesis of cell wall components were modulated, which suggested cell wall remodeling. In addition, alterations in the energy metabolism were observed. Furthermore, proteins involved in amino acid metabolism and hydrogen peroxide detoxification were modulated during osmotic stress. Our study suggests that P. lutzii Pb01. presents a vast osmoadaptative response that is composed of different proteins that act together to minimize the effects caused by osmotic stress.
authors: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, ICB-II s/n, Sala 206, Campus II, 74690-900 Goiânia, Goiás, Brazil.
authors: Leandro Nascimento da Silva
authors: LNDS
authors: Rodrigues
authors: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, ICB-II s/n, Sala 206, Campus II, 74690-900 Goiânia, Goiás, Brazil; Unidade Universitária de Ciências Exatas e Tecnológicas, Universidade Estadual de Goiás, BR153, 3105, Fazenda Barreiro do Meio, 75132-903 Anápolis, Goiás, Brazil; Centro Universitário de Anápolis, UniEvangélica, Avenida Universitária Km 3.5, Cidade Universitária, 75083-515 Anápolis, Goiás, Brazil. Electronic address: wesleyfarmacia@uol.com.br.
authors: Wesley de Almeida
authors: WA
authors: Brito
authors: Instituto de Ciências Fisiológicas, Universidade Federal do Amazonas, Avenida General Rodrigo Octávio, 6200, Coroado I, 69077-000 Manaus, Amazonas, Brazil. Electronic address: afparente@yahoo.com.br.
authors: Ana Flávia Alves
authors: AFA
authors: Parente
authors: Instituto de Ciências Exatas e Tecnologia, Universidade Federal do Amazonas, Rua Nossa Senhora do Rosário, 3863, 69103-128 Itacoatiara, Amazonas, Brazil. Electronic address: weberblood@gmail.com.
authors: Simone Schneider
authors: SS
authors: Weber
authors: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, ICB-II s/n, Sala 206, Campus II, 74690-900 Goiânia, Goiás, Brazil.
authors: Alexandre Melo
authors: AM
authors: Bailão
authors: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, ICB-II s/n, Sala 206, Campus II, 74690-900 Goiânia, Goiás, Brazil; Escola de Engenharia, Pontifícia Universidade Católica de Goiás, Avenida Universitária, 1440, Setor Universitário, 4605-010 Goiânia, Goiás, Brazil. Electronic address: lcasalet@gmail.com.
authors: Luciana
authors: L
authors: Casaletti
authors: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, ICB-II s/n, Sala 206, Campus II, 74690-900 Goiânia, Goiás, Brazil.
authors: Clayton Luiz
authors: CL
authors: Borges
authors: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, ICB-II s/n, Sala 206, Campus II, 74690-900 Goiânia, Goiás, Brazil. Electronic address: cmasoares@gmail.com.
authors: Célia Maria de Almeida
authors: CMA
authors: Soares
mesh_terms: D000222:Adaptation, Physiological; D000596:Amino Acids / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D004734:Energy Metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005936:Glucans / Q000096:biosynthesis; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D009997:Osmotic Pressure; D010228:Paracoccidioides / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D011189:Potassium Chloride / Q000494:pharmacology; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction
chemical_list: D000596:Amino Acids; D005656:Fungal Proteins; D005936:Glucans; D011189:Potassium Chloride; D006861:Hydrogen Peroxide; D005993:Glycerolphosphate Dehydrogenase; D005990:Glycerol
keywords: Amino acid metabolism; Cell wall remodeling; Glycerol production; Hyperosmotic stress; Osmoadaptation