title: Acute Alpha-Naphthylisothiocyanate-induced Liver Toxicity in Germfree and Conventional Male Rats.
abstract: Differences in the responses of conventional and germfree male Sprague-Dawley rats to acute injury induced by alpha-naphthylisothiocyanate (ANIT), a well-characterized biliary epithelial toxicant, were evaluated. Conventional and germfree rats were dosed once orally with 50 mg/kg of ANIT or corn oil alone and serially sacrificed daily for the next 3 days. Germfree rats treated with ANIT tended to have greater increases in virtually all liver and biliary-related analytes compared with conventional rats treated with ANIT; however, significant differences were found only in a few of these analytes including increased bile acids on day 3, total bilirubin on day 4, glutamate dehydrogenase (GLDH) on day 3, and reduced paraoxonase 1 (PON1) on days 2 and 3. Histologic differences between the conventional and germfree rats were modest, but most pronounced on day 2 (24-hr post dosing). Based on subjective scoring, biliary necrosis, neutrophilic cholangitis, and portal tract edema were more severe in germfree rats at 24 hr post dosing compared with conventional rats. Biliary epithelial replication did not differ between treated groups, however. Overall, germfree rats had a modestly greater level of biliary tract injury based on subjective histologic scoring and clinical chemistry measurements following an acute exposure to the well-characterized biliary toxin, ANIT; however, the difference between the ANIT-treated germfree and conventional groups was modest and most evident only within the first day following exposure. These findings suggest that the microbiome did not significantly affect ANIT-induced acute biliary tract injury in the conditions of this study.
authors: North Carolina State University, College of Veterinary Medicine, Raleigh, North Carolina, USA jcullen@ncsu.edu.
authors: John M
authors: JM
authors: Cullen
authors: GlaxoSmithKline, Safety Assessment, Research Triangle Park, North Carolina, USA.
authors: Brenda
authors: B
authors: Faiola
authors: GlaxoSmithKline, Safety Assessment, Research Triangle Park, North Carolina, USA.
authors: David H
authors: DH
authors: Melich
authors: GlaxoSmithKline, Safety Assessment, Research Triangle Park, North Carolina, USA.
authors: Richard A
authors: RA
authors: Peterson
authors: GlaxoSmithKline, Safety Assessment, Research Triangle Park, North Carolina, USA.
authors: Holly L
authors: HL
authors: Jordan
authors: GlaxoSmithKline, Statistical Sciences, Research Triangle Park, North Carolina, USA.
authors: Carie L
authors: CL
authors: Kimbrough
authors: GlaxoSmithKline, Safety Assessment, Research Triangle Park, North Carolina, USA.
authors: Richard T
authors: RT
authors: Miller
mesh_terms: D015058:1-Naphthylisothiocyanate / Q000633:toxicity*; D000818:Animals; D005856:Germ-Free Life / Q000187:drug effects*; D008099:Liver / Q000187:drug effects*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015058:1-Naphthylisothiocyanate
keywords: alpha-naphthylisothiocyanate; bile duct; germfree; liver; rat; toxicity