title: Storage and transportation of Prochilodus lineatus (Characiformes) sperm prior to cryopreservation.
abstract: In this study we compared post-thaw quality of P. lineatus sperm frozen shortly after collection, with sperm frozen after dilution and transportation, and up to 6h from collection. From each sperm sample (n=10 males) five aliquots were taken. One aliquot was diluted in the freezing medium (1 sperm:8 glucose:1 methyl glycol) and frozen ∼20min after collection in the field (control), while the other four aliquots were transported to the laboratory where freezing took place 3 or 6h after collection. From the transported aliquots, two were diluted 1:4 in glucose solution before transportation (diluted samples), while the other two were kept undiluted until freezing (undiluted samples). Thus the five treatments were: control, undiluted-3h, diluted-3h, undiluted-6h and diluted-6h. Post-thaw sperm was evaluated for membrane integrity, motility rate and velocities (curvilinear=VCL; average path=VAP; straight line=VSL). Post-thaw membrane integrity did not differ among the five treatments (48-60% intact sperm). Sperm motility rate was similar (P>0.05) between control (64%) and undiluted samples (60-62%) and higher (P<0.05) than that in diluted samples (35-45%), regardless the time after collection when freezing took place. Velocities were higher in control and in undiluted-3h samples (VCL of 254-265μm/s, VAP of 219-244μm/s and VSL of 134-147μm/s) than in diluted samples or samples frozen 6h after collection. P. lineatus sperm can be transported/shipped to the laboratory without decreasing its suitability for cryopreservation. Sperm should be kept undiluted during storage and be frozen within 3h.
authors: Animal Science Department, Federal University of Lavras, PO Box 3037, Lavras, MG 37200-000, Brazil. Electronic address: anatmviveiros@outlook.com.
authors: Ana T M
authors: AT
authors: Viveiros
authors: Animal Science Department, Federal University of Lavras, PO Box 3037, Lavras, MG 37200-000, Brazil.
authors: Isabela M
authors: IM
authors: Di Chiacchio
authors: Animal Science Department, Federal University of Lavras, PO Box 3037, Lavras, MG 37200-000, Brazil.
authors: Izabella L G
authors: IL
authors: Almeida
authors: Animal Science Department, Federal University of Lavras, PO Box 3037, Lavras, MG 37200-000, Brazil.
authors: Tais R
authors: TR
authors: Taffarel
authors: Animal Science Department, Federal University of Lavras, PO Box 3037, Lavras, MG 37200-000, Brazil.
authors: Marcelo C
authors: MC
authors: Leal
mesh_terms: D000818:Animals; D060125:Characiformes; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005615:Freezing; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013048:Specimen Handling; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013997:Time Factors; D014186:Transportation
keywords: CASA; Motility; Neotropical fish; Semen; Velocity