title: A Simple and Sensitive Method for Auramine O Detection Based on the Binding Interaction with Bovin Serum Albumin.
abstract: A simple, rapid and effective method for auramine O (AO) detection was proposed by fluorescence and UV-Vis absorption spectroscopy. In the BR buffer system (pH 7.0), AO had a strong quenching ability to the fluorescence of bovin serum albumin (BSA) by dynamic quenching. In terms of the thermodynamic parameters calculated as ΔH > 0 and ΔS > 0, the resulting binding of BSA and AO was mainly attributed to the hydrophobic interaction forces. The linearity of this method was in the concentration range from 0.16 to 50 μmol L(-1) with a detection limit of 0.05 μmol L(-1). Based on fluorescence resonance energy transfer (FRET), the distance r (1.36 nm) between donor (BSA) and acceptor (AO) was obtained. Furthermore, the effects of foreign substances and ionic strength were evaluated under the optimum reaction conditions. BSA as a selective probe could be applied to the analysis of AO in medicines with satisfactory results.
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University.
authors: Jingjing
authors: J
authors: Yan
authors: Xin
authors: X
authors: Huang
authors: Shaopu
authors: S
authors: Liu
authors: Jidong
authors: J
authors: Yang
authors: Yusheng
authors: Y
authors: Yuan
authors: Ruilin
authors: R
authors: Duan
authors: Hui
authors: H
authors: Zhang
authors: Xiaoli
authors: X
authors: Hu
mesh_terms: D001576:Benzophenoneidum / Q000032:analysis* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D009994:Osmolar Concentration; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D001576:Benzophenoneidum; D012710:Serum Albumin, Bovine