title: In Vitro Antileishmanial Activity of Sterols from Trametes versicolor (Bres. Rivarden).
abstract: Two ergostanes, 5α,8α-epidioxy-22E-ergosta-6,22-dien-3β-ol (1) and 5α-ergost-7,22-dien-3β-ol (2), and a lanostane, 3β-hydroxylanostan-8,24-diene-21-oic acid (trametenolic acid) (3), were isolated from an n-hexane extract prepared from the fruiting body of Trametes versicolor (Bres. Rivarden). The activity of the isolated sterols was evaluated against promastigotes and amastigotes of Leishmania amazonensis Lainson and Shaw, 1972. The lanostane, compound (3), showed the best inhibitory response (IC50 promastigotes 2.9 ± 0.1 μM and IC50 amastigotes 1.6 ± 0.1 μM). This effect was 25-fold higher compared with its cytotoxic effect on peritoneal macrophages from BALB/c mice. Therefore, trametenolic acid could be regarded as a promising lead for the synthesis of compounds with antileishmanial activity.
authors: Centre for Natural Product Studies, Faculty of Chemistry, University of Havana, Zapata y G, La Habana 10400, Cuba. leliebre@fq.uh.cu.
authors: Vivian
authors: V
authors: Leliebre-Lara
authors: Parasitology Department, Institute of Tropical Medicine "Pedro Kouri" Marianao 13, Havana 10400, Cuba. monzote@ipk.sld.cu.
authors: Lianet
authors: L
authors: Monzote Fidalgo
authors: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitaetsplatz 4, 8010 Graz, Austria. eva-maria.wenzig@uni-graz.at.
authors: Eva-Maria
authors: EM
authors: Pferschy-Wenzig
authors: Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Universitaetsplatz 4, 8010 Graz, Austria. olaf.kunert@uni-graz.at.
authors: Olaf
authors: O
authors: Kunert
authors: Centre for Natural Product Studies, Faculty of Chemistry, University of Havana, Zapata y G, La Habana 10400, Cuba. clara@fq.uh.cu.
authors: Clara
authors: C
authors: Nogueiras Lima
authors: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitaetsplatz 4, 8010 Graz, Austria. rudolf.bauer@uni-graz.at.
authors: Rudolf
authors: R
authors: Bauer
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D007891:Leishmania / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D013261:Sterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D055454:Trametes / Q000737:chemistry*; D014344:Trypanocidal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D013261:Sterols; D014344:Trypanocidal Agents
keywords: Trametes versicolor; antileishmanial activity; trametenolic acid B