title: Fabrication of anti-protein-fouling poly(ethylene glycol) microfluidic chip electrophoresis by sandwich photolithography.
abstract: Microfluidic chip electrophoresis (MCE) is a powerful separation tool for biomacromolecule analysis. However, adsorption of biomacromolecules, particularly proteins onto microfluidic channels severely degrades the separation performance of MCE. In this paper, an anti-protein-fouling MCE was fabricated using a novel sandwich photolithography of poly(ethylene glycol) (PEG) prepolymers. Photopatterned microchannel with a minimum resolution of 10 μm was achieved. After equipped with a conventional online electrochemical detector, the device enabled baseline separation of bovine serum albumin, lysozyme (Lys), and cytochrome c (Cyt-c) in 53 s under a voltage of 200 V. Compared with a traditional polydimethylsiloxane MCE made by soft lithography, the PEG MCE made by the sandwich photolithography not only eliminated the need of a master mold and the additional modification process of the microchannel but also showed excellent anti-protein-fouling properties for protein separation.
authors: Hailin
authors: H
authors: Cong
authors: College of Materials Science and Engineering, Qingdao University , Qingdao 266071, China.
authors: Xiaodan
authors: X
authors: Xu
authors: Bing
authors: B
authors: Yu
authors: Beijing National Laboratory for Molecular Sciences (BNLMS), College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors: Huwei
authors: H
authors: Liu
authors: College of Materials Science and Engineering, Qingdao University , Qingdao 266071, China.
authors: Hua
authors: 0000-0002-5414-2050
authors: H
authors: Yuan