title: IFITM3 and severe influenza virus infection. No evidence of genetic association.
abstract: Influenza virus infection (IVI) is typically subclinical or causes a self-limiting upper respiratory disease. However, in a small subset of patients IVI rapidly progresses to primary viral pneumonia (PVP) with respiratory failure; a minority of patients require intensive care unit admission. Inherited and acquired variability in host immune responses may influence susceptibility and outcome of IVI. However, the molecular basis of such human factors remains largely elusive. It has been proposed that homozygosity for IFITM3 rs12252-C is associated with a population-attributable risk of 5.4 % for severe IVI in Northern Europeans and 54.3 % for severe H1N1pdm infection in Chinese. A total of 148 patients with confirmed IVI were considered for recruitment; 118 Spanish patients (60 of them hospitalized with PVP) and 246 healthy Spanish individuals were finally included in the statistical analysis. PCR-RFLP was used with confirmation by Sanger sequencing. The allele frequency for rs12252-C was found to be 3.5 % among the general Spanish population. We found no rs12252-C homozygous individuals in our control group. The only Spanish patient homozygous for rs12252-C had a neurological disorder (a known risk factor for severe IVI) and mild influenza. Our data do not suggest a role of rs12252-C in the development of severe IVI in our population. These data may be relevant to recognize whether patients homozygous for rs12252-C are at risk of severe influenza, and hence require individualized measures in the case of IVI.
authors: Department of Immunology, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, 35010, Spain.|Department of Clinical Sciences, School of Medicine, Universidad de Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, 35016, Spain.
authors: M
authors: M
authors: López-Rodríguez
authors: Department of Immunology, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, 35010, Spain.|Department of Medical and Surgical Sciences, School of Medicine, Universidad de Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, 35016, Spain.
authors: E
authors: E
authors: Herrera-Ramos
authors: Intensive Care Unit, Hospital Universitario de Gran Canaria Dr. Negrín, CIBERES, Las Palmas de Gran Canaria, 35010, Spain.
authors: J
authors: J
authors: Solé-Violán
authors: Department of Internal Medicine, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas Gran Canaria, 35010, Spain.
authors: J J
authors: JJ
authors: Ruíz-Hernández
authors: Department of Respiratory Diseases, Hospital San Jorge, Huesca, 22004, Spain.
authors: L
authors: L
authors: Borderías
authors: Department of Infectious Diseases, Hospital Universitari del Mar, Barcelona, 08003, Spain.|Hospital del Mar de Investigaciones Médicas (IMIM), CIBERES, Barcelona, 08003, Spain.
authors: J P
authors: JP
authors: Horcajada
authors: Department of Infectious Diseases, Hospital Universitari del Mar, Barcelona, 08003, Spain.
authors: E
authors: E
authors: Lerma-Chippirraz
authors: Department of Respiratory Diseases, Hospital Universitario de la Princesa, Madrid, 28005, Spain.
authors: O
authors: O
authors: Rajas
authors: Department of Respiratory Diseases, Hospital Clínico y Universitario de Valencia, Valencia, 46010, Spain.
authors: M
authors: M
authors: Briones
authors: Department of Microbiology, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, 35010, Spain.
authors: M C
authors: MC
authors: Pérez-González
authors: Department of Statistics, Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, 35010, Spain.
authors: M A
authors: MA
authors: García-Bello
authors: Department of Immunology, Hospital La Paz, La Paz Institute of Biomedical Research, IdiPAZ, Madrid, 28046, Spain.
authors: E
authors: E
authors: López-Granados
authors: Department of Medical and Surgical Sciences, School of Medicine, Universidad de Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, 35016, Spain.|Department of Respiratory Diseases, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, 35010, Spain.
authors: F
authors: F
authors: Rodriguez de Castro
authors: Department of Immunology, Hospital Universitario Son Espases, Palma de Mallorca, 07120, Spain. josecarlos.rodriguezgallego@ssib.es.|Department of Immunology, Hospital Universitario Son Espases, Carretera de Valldemossa 79, 07120, Palma de Mallorca, Spain. josecarlos.rodriguezgallego@ssib.es.
authors: C
authors: C
authors: Rodríguez-Gallego
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006801:Humans; D007251:Influenza, Human / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016601:RNA-Binding Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013030:Spain; D055815:Young Adult
chemical_list: C080082:IFITM3 protein, human; D008565:Membrane Proteins; D016601:RNA-Binding Proteins