title: 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone inhibits LPS-induced BV2 microglial activation through MAPK/NF-kB signaling pathways.
abstract: OBJECTIVE
  To verify the effects of several 5,8-dimethoxy-1,4-naphthoquinone (DMNQ) derivatives on LPS-induced NO production, cellular ROS levels and cytokine expression in BV-2 microglial cells.
  
  
  METHODS
  An MTT assay and FACS flow cytometry were performed to assess the cellular viability and apoptosis and cellular ROS levels, respectively. To examine the expression of pro-inflammatory cytokines and cellular signaling pathways, semi-quantitative RT-PCR and Western blotting were also used in this study.
  
  
  RESULTS
  Among the six newly synthesized DMNQ derivatives, 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone (R6) significantly inhibited the NO production, cellular ROS levels and the cytokines expression in BV-2 microglial cells, which stimulated by LPS. Signaling study showed that compound R6 treatment also significantly down-regulated the LPS-induced phosphorylation of MAPKs (ERK, JNK and p38) and decreased the degradation of IκB-α in BV2 microglial cells.
  
  
  CONCLUSIONS
  Our findings demonstrate that our newly synthesized compound derived from DMNQ, 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone (R6), might be a therapeutic agent for the treatment of glia-mediated neuroinflammatory diseases.
authors: College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
authors: Hu-Nan
authors: HN
authors: Sun
authors: College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
authors: Gui-Nan
authors: GN
authors: Shen
authors: Yan Bian University Health Science Center, Yanji 133000, China.
authors: Yong-Zhe
authors: YZ
authors: Jin
authors: Yan Bian University Health Science Center, Yanji 133000, China.
authors: Yu
authors: Y
authors: Jin
authors: College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
authors: Ying-Hao
authors: YH
authors: Han
authors: College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
authors: Li
authors: L
authors: Feng
authors: College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
authors: Lei
authors: L
authors: Liu
authors: College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
authors: Mei-Hua
authors: MH
authors: Jin
authors: College of Animal Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
authors: Ying-Hua
authors: YH
authors: Luo
authors: New Drug Development Center, Osong Medical Innovation Foundation, 123 Osongsaengmyeong-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungbuk, 363-951, Republic of Korea.
authors: Tea-Ho
authors: TH
authors: Kwon
authors: College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
authors: Yu-Dong
authors: YD
authors: Cui
authors: College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
authors: Cheng-Hao
authors: CH
authors: Jin
keywords: Biochemistry; Cell biology; Immunology; Medicine; Neuroscience