title: In Vitro Bioactivity Study of RGD-Coated Titanium Alloy Prothesis for Revision Total Hip Arthroplasty.
abstract: Total hip arthroplasty (THA) is a common procedure for the treatment of end-stage hip joint disease, and the demand for revision THA will double by 2026. Ti6Al4V (Titanium, 6% Aluminum, and 4% Vanadium) is a kind of alloy commonly used to make hip prothesis. To promote the osseointegration between the prothesis and host bone is very important for the revision THA. The peptide Arg-Gly-Asp (RGD) could increase cell attachment and has been used in the vascular tissue engineering. In this study, we combined the RGD with Ti6Al4V alloy using the covalent cross-linking method to fabricate the functional Ti6Al4V alloy (FTA). The distribution of RGD oligopeptide on the FTA was even and homogeneous. The FTA scaffolds could promote mouse osteoblasts adhesion and spreading. Furthermore, the result of RT-qPCR indicated that the FTA scaffolds were more beneficial to osteogenesis, which may be due to the improvement of osteoblast adhesion by the RGD oligopeptide coated on FTA. Overall, the FTA scaffolds developed herein pave the road for designing and building more efficient prothesis for osseointegration between the host bone and prothesis in revision THA.
authors: Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province 250021, China.
authors: Zhentao
authors: Z
authors: Man
authors: Department of Medical Oncology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province 250021, China.
authors: Dan
authors: D
authors: Sha
authors: Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province 250021, China.
authors: Shui
authors: S
authors: Sun
authors: Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province 250021, China.
authors: Tao
authors: T
authors: Li
authors: Department of Orthopaedics, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong Province 250013, China.
authors: Bin
authors: B
authors: Li
authors: Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province 250021, China.
authors: Guang
authors: G
authors: Yang
authors: Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province 250021, China.
authors: Laibo
authors: 0000-0002-2075-5297
authors: L
authors: Zhang
authors: Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province 250021, China.
authors: Changshun
authors: C
authors: Wu
authors: Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province 250021, China.
authors: Peng
authors: P
authors: Jiang
authors: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province 250021, China.
authors: Xiaojuan
authors: 0000-0003-1154-3409
authors: X
authors: Han
authors: Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province 250021, China.
authors: Wei
authors: 0000-0002-4731-3632
authors: W
authors: Li
mesh_terms: D000497:Alloys; D000818:Animals; D001178:Arthroplasty / Q000379:methods; D019644:Arthroplasty, Replacement, Hip / Q000379:methods; D002448:Cell Adhesion / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage*; D006615:Hip / Q000601:surgery*; D006621:Hip Joint / Q000601:surgery*; D006622:Hip Prosthesis; D016876:Metal Ceramic Alloys / Q000627:therapeutic use*; D051379:Mice; D009842:Oligopeptides / Q000008:administration & dosage*; D010006:Osteoblasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014025:Titanium / Q000008:administration & dosage*
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D016876:Metal Ceramic Alloys; D009842:Oligopeptides; C031462:titanium alloy (TiAl6V4); C047981:arginyl-glycyl-aspartic acid; D014025:Titanium