title: Distinct Roles of PKCι/λ and PKMζ in the Initiation and Maintenance of Hippocampal Long-Term Potentiation and Memory.
abstract: PKMζ has been proposed to be essential for maintenance of long-term potentiation (LTP) and long-term memory (LTM). However, recent data from PKMζ-knockout mice has called this role into question. Instead, the other atypical isoform, protein kinase C iota/lambda (PKCι/λ), has emerged as a potential alternative player. Therefore, the nature of the "memory molecule" maintaining learned information remains uncertain. Here, we report knockdown (KD) of PKCι/λ and PKMζ in the dorsal hippocampus and find deficits in early expression and late maintenance, respectively, during both LTP and hippocampus-dependent LTM. Sequential increases in the active form of PKCι/λ and PKMζ are detected during LTP or fear conditioning. Importantly, PKMζ, but not PKCι/λ, KD disrupts previously established LTM. Thus, PKCι/λ and PKMζ have distinct functions in LTP and memory, with PKMζ playing a specific role in memory maintenance. This relaying pattern may represent a precise molecular mechanism by which atypical PKCs regulate the different stages of memory.
authors: The Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, Nanjing, Jiangsu Province 210096, China; Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu Province 210029, China.
authors: Shaoli
authors: S
authors: Wang
authors: The Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, Nanjing, Jiangsu Province 210096, China; Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu Province 210029, China.
authors: Tao
authors: T
authors: Sheng
authors: Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu Province 210029, China.
authors: Siqiang
authors: S
authors: Ren
authors: Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu Province 210029, China.
authors: Tian
authors: T
authors: Tian
authors: The Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, Nanjing, Jiangsu Province 210096, China; Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu Province 210029, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province 226001, China. Electronic address: luwei@seu.edu.cn.
authors: Wei
authors: W
authors: Lu
mesh_terms: D000818:Animals; D005239:Fear / Q000502:physiology; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000033:anatomy & histology / Q000502:physiology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D017774:Long-Term Potentiation / Q000235:genetics*; D008297:Male; D057567:Memory, Long-Term / Q000502:physiology*; D008570:Memory, Short-Term / Q000502:physiology*; D008867:Microtomy; D018832:Molecular Chaperones / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D046509:Tissue Culture Techniques; D000099293:Protein Kinase C-lambda; D000099221:Protein Kinase C zeta
chemical_list: D049452:Green Fluorescent Proteins; D007527:Isoenzymes; D018832:Molecular Chaperones; D011493:Protein Kinase C; D034741:RNA, Small Interfering; D000099293:Protein Kinase C-lambda; C490392:Prkcz protein, rat; D000099221:Protein Kinase C zeta; C485184:enhanced green fluorescent protein