title: An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity.
abstract: The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer. Having improved the chemical synthesis and purification of the fluorescently-labelled DMB-DP3 acceptor, we report optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human polysialyltransferase activity.
authors: Institute of Cancer Therapeutics, Faculty of Life Sciences, University of Bradford, Bradford BD7 1DP, UK. r.a.falconer1@bradford.ac.uk.
authors: Sara M
authors: SM
authors: Elkashef
authors: Mark
authors: M
authors: Sutherland
authors: Laurence H
authors: LH
authors: Patterson
authors: Paul M
authors: PM
authors: Loadman
authors: Robert A
authors: RA
authors: Falconer
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D012799:Sialyltransferases / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D012799:Sialyltransferases