title: New insights into Legionella pneumophila biofilm regulation by c-di-GMP signaling.
abstract: The waterborne pathogen Legionella pneumophila grows as a biofilm, freely or inside amoebae. Cyclic-di-GMP (c-di-GMP), a bacterial second messenger frequently implicated in biofilm formation, is synthesized and degraded by diguanylate cyclases (DGCs) and phosphodiesterases (PDEs), respectively. To characterize the c-di-GMP-metabolizing enzymes involved in L. pneumophila biofilm regulation, the consequences on biofilm formation and the c-di-GMP concentration of each corresponding gene inactivation were assessed in the Lens strain. The results showed that one DGC and two PDEs enhance different aspects of biofilm formation, while two proteins with dual activity (DGC/PDE) inhibit biofilm growth. Surprisingly, only two mutants exhibited a change in global c-di-GMP concentration. This study highlights that specific c-di-GMP pathways control L. pneumophila biofilm formation, most likely via temporary and/or local modulation of c-di-GMP concentration. Furthermore, Lpl1054 DGC is required to enable the formation a dense biofilm in response to nitric oxide, a signal for biofilm dispersion in many other species.
authors: a Laboratoire de Génie chimique UMR 5503 , UPS, Université de Toulouse , Toulouse , France.
authors: Sophie
authors: S
authors: Pécastaings
authors: b CIRI, International Center for Infectiology Research , Legionella Pathogenesis Group, Université de Lyon , Lyon , France.|c Inserm , Lyon , France.|d Ecole Normale Supérieure de Lyon , Lyon , France.|e Université Lyon 1, CIRI, International Center for Infectiology Research , Lyon , France.|f CNRS , Lyon , France.
authors: Julie
authors: J
authors: Allombert
authors: a Laboratoire de Génie chimique UMR 5503 , UPS, Université de Toulouse , Toulouse , France.
authors: Barbora
authors: B
authors: Lajoie
authors: b CIRI, International Center for Infectiology Research , Legionella Pathogenesis Group, Université de Lyon , Lyon , France.|c Inserm , Lyon , France.|d Ecole Normale Supérieure de Lyon , Lyon , France.|e Université Lyon 1, CIRI, International Center for Infectiology Research , Lyon , France.|f CNRS , Lyon , France.
authors: Patricia
authors: P
authors: Doublet
authors: a Laboratoire de Génie chimique UMR 5503 , UPS, Université de Toulouse , Toulouse , France.
authors: Christine
authors: C
authors: Roques
authors: b CIRI, International Center for Infectiology Research , Legionella Pathogenesis Group, Université de Lyon , Lyon , France.|c Inserm , Lyon , France.|d Ecole Normale Supérieure de Lyon , Lyon , France.|e Université Lyon 1, CIRI, International Center for Infectiology Research , Lyon , France.|f CNRS , Lyon , France.
authors: Anne
authors: A
authors: Vianney
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D016952:Legionella pneumophila / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C062025:bis(3',5')-cyclic diguanylic acid; D010727:Phosphoric Diester Hydrolases; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase; D006152:Cyclic GMP
keywords: Biofilm; Legionella; NO response; c-di-GMP signaling; second messenger