title: C18-attached membrane funnel-based spray ionization mass spectrometry for quantification of anti-diabetic drug from human plasma.
abstract: In this work, sorbent-attached membrane funnel-based spray ionization mass spectrometry was explored for quantitative analysis of anti-diabetic drugs spiked in human plasma. C18-attached membrane funnel was fabricated for in situ extraction and clean-up to alleviate matrix suppression effect in the ionization process. Repaglinide was used as a target analyte of anti-diabetic drugs. Under optimal working conditions, good linearity (R(2) > 0.99) was obtained in the concentration range of 1-100 ng mL(-1). The method detection limit of target drugs spiked in the human plasma was around 0.30 ng mL(-1). Through the application of an isotope-labeled internal standard, the signal fluctuation caused by residual background matrices was largely alleviated and the precision of measurement (RSD) was below 15%. The recovery of repaglinide for 5, 25, and 100 ng mL(-1) of spiked human plasma matrixes ranged from 87% to 112%. The developed method was successfully applied to determine repaglinide in plasma volunteers who orally received a dose of drug association. Our results demonstrated that membrane funnel-based spray is a simple and sensitive method for rapid screening analysis of complex biological samples.
authors: Department of Chemistry, The Chinese University of Hong Kong, Hong Kong Special Administrative Region.
authors: Wan
authors: W
authors: Li
authors: Department of Chemistry, The Chinese University of Hong Kong, Hong Kong Special Administrative Region; Shandong Analysis and Test Centre, Shandong Academy of Sciences, Jinan, Shandong, PR China. Electronic address: xiangfchensdas@163.com.
authors: Xiangfeng
authors: X
authors: Chen
authors: Department of Chemistry, The Chinese University of Hong Kong, Hong Kong Special Administrative Region.
authors: Y-L Elaine
authors: YL
authors: Wong
authors: Department of Chemistry, The Chinese University of Hong Kong, Hong Kong Special Administrative Region.
authors: Y-L Winnie
authors: YL
authors: Hung
authors: Department of Chemistry, The Chinese University of Hong Kong, Hong Kong Special Administrative Region.
authors: Ze
authors: Z
authors: Wang
authors: Department of Chemistry, The Chinese University of Hong Kong, Hong Kong Special Administrative Region.
authors: Liulin
authors: L
authors: Deng
authors: Department of Chemistry, The Chinese University of Hong Kong, Hong Kong Special Administrative Region. Electronic address: twdchan@cuhk.edu.hk.
authors: T-W
authors: TW
authors: Dominic Chan
mesh_terms: D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood*; D008297:Male; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation
chemical_list: D007004:Hypoglycemic Agents
keywords: Ambient ionization; Anti-diabetic drug; Human plasma; Mass spectrometry; Solid-phase extraction