title: Promotion of formyl peptide receptor 1-mediated neutrophil chemotactic migration by antimicrobial peptides isolated from the centipede Scolopendra subspinipes mutilans.
abstract: We investigated the effects of two antimicrobial peptides (AMPs) isolated from Scolopendra subspinipes mutilans on neutrophil activity. Stimulation of mouse neutrophils with the two AMPs elicited chemotactic migration of the cells in a pertussis toxin-sensitive manner. The two AMPs also stimulated activation of ERK and Akt, which contribute to chemotactic migration of neutrophils. We found that AMP-stimulated neutrophil chemotaxis was blocked by a formyl peptide receptor (FPR) 1 antagonist (cyclosporin H); moreover the two AMPs stimulated the chemotactic migration of FPR1-expressing RBL-2H3 cells but not of vector-expressing RBL-2H3 cells. We also found that the two AMPs stimulate neutrophil migration in vivo, and that this effect is blocked in FPR1-deficient mice. Taken together, our results suggest that the two AMPs stimulate neutrophils, leading to chemotactic migration through FPR1, and the two AMPs will be useful for the study of FPR1 signaling and neutrophil activation. [BMB Reports 2016; 49(9): 520-525].
authors: Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea.
authors: Yoo Jung
authors: YJ
authors: Park
authors: Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea.
authors: Sung Kyun
authors: SK
authors: Lee
authors: Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea.
authors: Young Su
authors: YS
authors: Jung
authors: Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul 06351, Korea.
authors: Mingyu
authors: M
authors: Lee
authors: Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea.
authors: Ha Young
authors: HY
authors: Lee
authors: Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea.
authors: Sang Doo
authors: SD
authors: Kim
authors: Department of Hematology-Oncology, Ajou University School of Medicine, Suwon 16499, Korea.
authors: Joon Seong
authors: JS
authors: Park
authors: Department of Brain and Cognitive Sciences, DGIST, Daegu 42988, Korea.
authors: JaeHyung
authors: J
authors: Koo
authors: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 55365, Korea.
authors: Jae Sam
authors: JS
authors: Hwang
authors: Department of Biological Sciences, Sungkyunkwan University, Suwon 16419; Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul 06351, Korea.
authors: Yoe-Sik
authors: YS
authors: Bae
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D015153:Blotting, Western; D002460:Cell Line; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D016572:Cyclosporine / Q000494:pharmacology; D000077743:Diterpene Alkaloids; D004365:Drugs, Chinese Herbal / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D037342:Pertussis Toxin / Q000633:toxicity; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D044042:Receptors, Formyl Peptide / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D000077743:Diterpene Alkaloids; D004365:Drugs, Chinese Herbal; C469988:Fpr1 protein, mouse; D044042:Receptors, Formyl Peptide; D016572:Cyclosporine; D037342:Pertussis Toxin; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; C050025:cyclosporin H; C574917:scolopendra alkaloids