title: Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells.
abstract: Cervical cancer is one of the most common cancers in women worldwide and it is a prominent cause of cancer mortality. Curcumin is one of the major compounds from Turmeric and has been shown to induce cytotoxic cell death in human cervical cancer cells. However, there is no study to show curcumin induced DNA damage action via the effect on the DNA damage and repair protein in cervical cancer cells in detail. In this study, we investigated whether or not curcumin induced cell death via DNA damage, chromatin condensation in human cervical cancer HeLa cells by using comet assay and DAPI staining, respectively, we found that curcumin induced cell death through the induction of DNA damage, and chromatin condensation. Western blotting and confocal laser microscopy examination were used to examine the effects of curcumin on protein expression associated with DNA damage, repair and translocation of proteins. We found that curcumin at 13 µM increased the protein levels associated with DNA damage and repair, such as O6-methylguanine-DNA methyltransferase, early-onset breast cancer 1 (BRCA1), mediator of DNA damage checkpoint 1, p-p53 and p-H2A.XSer140 in HeLa cells. Results from confocal laser systems microscopy indicated that curcumin increased the translocation of p-p53 and p-H2A.XSer140 from cytosol to nuclei in HeLa cells. In conclusion, curcumin induced cell death in HeLa cells via induction of DNA damage, and chromatin condensation in vitro.
authors: Department of Pathology, National Defense Medical Center, Division of Clinical Pathology, Tri-Service General Hospital, Taipei, Taiwan, R.O.C.
authors: Hung-Sheng
authors: HS
authors: Shang
authors: Department of Surgical Oncology, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C.
authors: Chuan-Hsun
authors: CH
authors: Chang
authors: Department of Nutrition Therapy, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C.
authors: Yu-Ru
authors: YR
authors: Chou
authors: Department of Office of Director, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C.
authors: Ming-Yang
authors: MY
authors: Yeh
authors: Department of Orthopedics, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C.
authors: Man-Kuan
authors: MK
authors: Au
authors: Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C.
authors: Hsu-Feng
authors: HF
authors: Lu
authors: International Master's Degree Program in Food Science, International College, National Pingtung University of Science and Technology, Pingtung, Taiwan, R.O.C.
authors: Yung-Lin
authors: YL
authors: Chu
authors: Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, R.O.C.
authors: Hsiao-Min
authors: HM
authors: Chou
authors: Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, R.O.C.
authors: Hsiu-Chen
authors: HC
authors: Chou
authors: Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, R.O.C.
authors: Yung-Luen
authors: YL
authors: Shih
authors: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
authors: Jing-Gung
authors: JG
authors: Chung
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D019313:BRCA1 Protein / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002843:Chromatin / Q000235:genetics; D003474:Curcumin / Q000008:administration & dosage*; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology
chemical_list: D019313:BRCA1 Protein; D002843:Chromatin; D009363:Neoplasm Proteins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D019853:O(6)-Methylguanine-DNA Methyltransferase; D003474:Curcumin