title: Effects of dietary 2,2', 4,4'-tetrabromodiphenyl ether (BDE-47) exposure in growing medaka fish (Oryzias latipes).
abstract: In this research work, we addressed the effects of a diet fortified with BDE-47 (0, 10, 100, 1000ng/g) dosed to 4-7 day-old post-hatch medaka fish for 40 days, followed by an 80-day depuration period. BDE-47 accumulation and overall growth were evaluated throughout the dosing period, and its elimination was quantified over the following 60 days. The histological condition of the thyroid gland, liver and gonads from the 1000ng BDE-47-treated fish were assessed 5 and 70days after exposures finished. The phenotypic males to females ratio was also quantified 70days after treatments finished. Sixty days after the BDE-47 exposures, reproductive capacity (i.e. fecundity, fertility and hatchability) was evaluated in mating groups for a 20-day period. BDE-47 exposure via food from larval through juvenile life stages of medaka fish resulted in steady accumulation with time dose-dependently. This accumulation tendency rapidly decreased after dosing ended. The growth rates showed a significant increase only at the highest concentration 70days after exposures finished. The histological survey did not reveal BDE-47-related alterations in the condition of the potential target organs. However, a morphometrical approach suggested BDE-47-related differences in the thickness of the epithelium that lines thyroid follicles. The reproduction studies showed comparable values for the fecundity, fertility and hatching rates. Dietary BDE-47 dosed for 40days to growing medaka fish did not alter the phenotypic sex ratios at maturity. The dietary approach used herein could not provide conclusive evidence of effects on medaka development and thriving despite the fact that BDE-47 underwent rapid accumulation in whole fish during the 40-day treatment.
authors: Laboratory for Ecotoxicology, Department of the Environment, National Institute for Agricultural and Food Research and Technology, A-6, Km. 7.5, E-28040 Madrid, Spain. Electronic address: miguelgd@inia.es.
authors: Miguel
authors: M
authors: González-Doncel
authors: Laboratory for Ecotoxicology, Department of the Environment, National Institute for Agricultural and Food Research and Technology, A-6, Km. 7.5, E-28040 Madrid, Spain.
authors: Gregoria
authors: G
authors: Carbonell
authors: Department of Cell Biology, School of Medicine, Complutense University, Ciudad Universitaria, E-28040 Madrid, Spain.
authors: José Enrique
authors: JE
authors: García-Mauriño
authors: Laboratory of Forest Soils, Department of Forest Ecology, National Institute for Agricultural and Food Research and Technology, A-6, Km. 7.5, E-28040 Madrid, Spain.
authors: Salvador
authors: S
authors: Sastre
authors: Laboratory for Ecotoxicology, Department of the Environment, National Institute for Agricultural and Food Research and Technology, A-6, Km. 7.5, E-28040 Madrid, Spain.
authors: Eulalia María
authors: EM
authors: Beltrán
authors: Laboratory for Ecotoxicology, Department of the Environment, National Institute for Agricultural and Food Research and Technology, A-6, Km. 7.5, E-28040 Madrid, Spain.
authors: Carlos
authors: C
authors: Fernàndez Torija
mesh_terms: D000818:Animals; D004032:Diet; D005260:Female; D006066:Gonads / Q000187:drug effects / Q000473:pathology; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D009990:Oryzias / Q000254:growth & development / Q000502:physiology*; D010641:Phenotype; D012098:Reproduction / Q000187:drug effects*; D013961:Thyroid Gland / Q000187:drug effects / Q000473:pathology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D055768:Halogenated Diphenyl Ethers; D014874:Water Pollutants, Chemical; C511295:2,2',4,4'-tetrabromodiphenyl ether
keywords: Bioaccumulation; Growth; Polybrominated diphenyl ethers; Reproduction