title: Construction and application of the Synechocystis sp. PCC6803-ftnA in microbial contamination control in a coupled cultivation and wastewater treatment.
abstract: Inspired by iron fertilization experiments in HNLC (high-nitrate, low-chlorophyll) sea areas, we proposed the use of iron-rich engineered microalgae for microbial contaminant control in iron-free culture media. Based on the genome sequence and natural transformation system of Synechocystis sp. PCC6803, ftnA (encoding ferritin) was selected as our target gene and was cloned into wild-type Synechocystis sp. PCC6803. Tests at the molecular level confirmed the successful construction of the engineered Synechocystis sp. PCC6803-ftnA. After Fe(3+)-EDTA pulsing, the intracellular iron content of Synechocystis sp. PCC6803-ftnA was significantly enhanced, and the algae was used in the microbial contamination control system. In the coupled Synechocystis sp. PCC6803-ftnA production and municipal wastewater (MW, including Scenedesmus obliquus and Bacillus) treatment, Synechocystis sp. PCC6803-ftnA accounted for all of the microbial activity and significantly increased from 70% of the microbial community to 95%. These results revealed that while the stored iron in the Synechocystis sp. PCC6803-ftnA cells was used for growth and reproduction of this microalga in the MW, the growth of other microbes was inhibited because of the iron limitation, and these results provide a new method for microbial contamination control during a coupling process.
authors: State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze Water Environment of Ministry of Education, Tongji University, Shanghai 200092, China. Electronic address: zhangyalei@tongji.edu.cn.
authors: Yalei
authors: Y
authors: Zhang
authors: State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze Water Environment of Ministry of Education, Tongji University, Shanghai 200092, China; Water Conservancy Development Research Center, Taihu Basin Authority of Ministry of Water Resources, Shanghai 200434, China.
authors: Chunmin
authors: C
authors: Zhang
authors: State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze Water Environment of Ministry of Education, Tongji University, Shanghai 200092, China. Electronic address: zhouxuefei@tongji.edu.cn.
authors: Xuefei
authors: X
authors: Zhou
authors: State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze Water Environment of Ministry of Education, Tongji University, Shanghai 200092, China.
authors: Zheng
authors: Z
authors: Shen
authors: State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze Water Environment of Ministry of Education, Tongji University, Shanghai 200092, China.
authors: Fangchao
authors: F
authors: Zhao
authors: State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze Water Environment of Ministry of Education, Tongji University, Shanghai 200092, China.
authors: Jianfu
authors: J
authors: Zhao
mesh_terms: D046939:Synechocystis / Q000502:physiology*; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000382:microbiology*
chemical_list: D062065:Wastewater
keywords: Microbial contaminants; Municipal wastewater treatment; Synechocystis PCC6803-ftnA