title: Effect of Laminin on Neurotrophic Factors Expression in Schwann-Like Cells Induced from Human Adipose-Derived Stem Cells In Vitro.
abstract: The Schwann-like cells can be considered as promising in stem cell therapies, at least in experimental models. Human adipose-derived stem cells (ADSCs) are induced into Schwann-like cells (SC-like cells) and are cultured on either a plastic surface or laminin-coated plates. The findings here reveal that laminin is a critical component in extracellular matrix (ECM) of SC-like cells at in vitro. The survival rate of SC-like cells on a laminin matrix are measured through MTT assay and it is found that this rate is significantly higher than that of the cells grown on a plastic surface (P < 0.05). Schwann cell markers and the myelinogenic ability of SC-like cells at the presence versus absence of laminin are assessed through immunocytochemistry. The analysis of GFAP/S100β and S100β/MBP markers indicate that laminin can increase the differentiated rate and myelinogenic potential of SC-like cells. The expression levels of SCs markers, myelin basic proteins (MBP), and neurotrophic factors in two conditions are analyzed by real-time reverse transcription polymerase chain reaction (RT-PCR). The findings here demonstrated that gene expression of SCs markers, MBP, and brain-derived neurotrophic factors (BDNF) increase significantly on laminin compared to plastic surface (P < 0.01). In contrast, the nerve growth factor (NGF) expression is downregulated significantly on laminin-coated plates (P < 0.05). The obtained data suggest that production of neurotrophic factors in SC-like cell in presence of laminin can induce appropriate microenvironment for nerve repair in neurodegenerative diseases.
authors: Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81744-176, Iran.|Department of Anatomical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, 71345-1731, Iran.
authors: Giti
authors: G
authors: Zarinfard
authors: Department of Anatomical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, 71345-1731, Iran.
authors: Mina
authors: M
authors: Tadjalli
authors: Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81744-176, Iran. razavi@med.mui.ac.ir.
authors: Shahnaz
authors: S
authors: Razavi
authors: Department of Molecular Biology and Genetic, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Mohammad
authors: M
authors: Kazemi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008875:Middle Aged; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D005904:Glial Fibrillary Acidic Protein; D007797:Laminin; D004676:Myelin Basic Protein; D009418:S100 Proteins; D020932:Nerve Growth Factor
keywords: Adipose-derived stem cells; Laminin; Myelin; Neurotrophic factors; Schwann cell