title: Chromatin Immunoprecipitation to Study The Plant Epigenome.
abstract: Chromatin immunoprecipitation (ChIP) has been widely used for studying in vivo protein-DNA interactions for decades. ChIP is a powerful tool that is adaptable for studying epigenetic modifications at certain genomic loci or the genomic level. Given its utility in studying the epigenome and the many technical challenges, we present a detailed in-lab ChIP protocol primarily used for studying histone modifications in plants, but can be easily adapted for other chromatin targets in other species.
authors: Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 218 Agricultural Sciences Bldg, 1955 East-West Road, Honolulu, HI, 96822, USA. zidianx@hawaii.edu.
authors: Zidian
authors: Z
authors: Xie
authors: Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 218 Agricultural Sciences Bldg, 1955 East-West Road, Honolulu, HI, 96822, USA.
authors: Gernot
authors: G
authors: Presting
mesh_terms: D002843:Chromatin; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics; D044127:Epigenesis, Genetic; D057890:Epigenomics / Q000379:methods*; D006657:Histones / Q000737:chemistry* / Q000378:metabolism; D013025:Glycine max / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
chemical_list: D002843:Chromatin; D018744:DNA, Plant; D006657:Histones
keywords: Antibody; Chromatin immunoprecipitation; Cross-linking; Epigenetic modifications; Epigenome; Histone