title: The Effects of Matrix Metalloproteinase-9 on Dairy Goat Mastitis and Cell Survival of Goat Mammary Epithelial Cells.
abstract: Matrix metalloproteinase-9 (MMP-9) is a zinc-dependent enzyme, and plays a crucial role in extracellular matrix degeneration, inflammation and tissue remodeling. However, the relationship between MMP-9 and somatic cell count (SCC) in goat milk and the role of MMP-9 in the regulation of mastitis are still unknown. In this study, we found MMP-9 was predominantly expressed in the spleen, intestine and mammary gland. The SCC in goat milk was positively correlated with MMP-9 expression, and staphylococcus aureus could markedly increase MMP-9 expression in goat mammary epithelial cells (GMEC) in dosage and time dependent manner. We also demonstrated that SB-3CT, an inhibitor of MMP-9, promoted apoptosis and inhibited proliferation in GMEC. Thus, MMP-9 may emerge as an easily measurable and sensitive parameter that reflects the number of somatic cells present in milk and a regulatory factor of apoptosis in GMEC.
authors: College of Animal Science and Technology, Northwest A&F University, Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, China.
authors: Hui
authors: H
authors: Li
authors: College of Animal Science and Technology, Northwest A&F University, Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, China.
authors: Huiling
authors: H
authors: Zheng
authors: College of Animal Science and Technology, Northwest A&F University, Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, China.
authors: Lihui
authors: L
authors: Li
authors: College of Animal Science and Technology, Northwest A&F University, Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, China.
authors: Xingai
authors: X
authors: Shen
authors: College of Animal Science and Technology, Northwest A&F University, Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, China.
authors: Wenjuan
authors: W
authors: Zang
authors: College of Animal Science and Technology, Northwest A&F University, Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, China.
authors: Yongsen
authors: Y
authors: Sun
mesh_terms: D000818:Animals; D017209:Apoptosis; D002452:Cell Count; D002470:Cell Survival; D003612:Dairying; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006041:Goats; D008321:Mammary Glands, Animal / Q000382:microbiology / Q000473:pathology*; D008413:Mastitis / Q000201:enzymology* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000502:physiology
chemical_list: D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9