title: Advances in LC: bioanalytical method transfer.
abstract: There are three main reasons for transferring from an existing bioanalytical assay to an alternative chromatographic method: speed, cost and sensitivity. These represent a challenge to the analyst in that there is an interplay between these three considerations and one factor is often improved at the expense of another. These three factors act as drivers to encourage technology development and support its uptake. The more recently introduced chromatographic technologies may show significant improvements against one of more of these factors relative to conventional 4.6-mm id reversed-phase HPLC. In this article, some of these new chromatographic approaches will be considered in terms of what they can offer the bioanalysts.
authors: Keele University Institute of Science & Technology in Medicine, Guy Hilton Research Centre, Thornburrow Drive, Hartshill, Stoke-on-Trent, ST4 7QB UK.
authors: Patricia
authors: P
authors: Wright
authors: Cyclofluidic Ltd, Biopark, Broadwater Road, Welwyn Garden City, Herts., AL7 3AX UK.
authors: Adrian
authors: A
authors: Wright
mesh_terms: D000465:Algorithms; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000191:economics / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D008904:Miniaturization / Q000295:instrumentation / Q000379:methods; D054928:Validation Studies as Topic
keywords: HPLC; MS; SFC; UHPLC; bioanalysis; selectivity; sensitivity; validation