title: Highly sensitive electrochemiluminescent immunosensor based on gold nanoparticles-functionalized zinc oxide nanorod and poly(amidoamine)-graphene for detecting brombuterol.
abstract: β-adrenergic agonists (β-agonists) recognized as a growth promoter will reflect the health of human. Sensitive detection of β-agonists in foodstuff is valuable for the health of animals and human. A novel ultrasensitive competition-type electrochemiluminescent (ECL) immunosensor was developed for detecting brombuterol (Brom) based on CdTe Quantum dot (QDs) and polyamidoamine dendrimer (PAMAM, G2) modified graphene oxide (GO) (CdTe QDs-PAMAM-GO composite) as bioprobe for the first time. The surface of glassy carbon electrode (GCE) was coated with AuNPs-ZnO NRs composite film as the platform, which facilitated the electronic transmission rate to enhance the ECL intensity and provide enough active sites for capturing antibody. The resulting ECL immunosensor enabled the real samples detection of Brom with a lower detection limit of 0.3pgmL(-1) (S/N=3) and a wider linear range from 0.001 to 500ngmL(-1). The proposed immunosensor coupled with the excellent advantages of CdTe QDs-PAMAM-GO and AuNPs-ZnO NRs composite displayed high sensitivity and long-term stability, and provided an approach for determining other important biomarkers.
authors: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou, 215123 China.
authors: Qing
authors: Q
authors: Zhu
authors: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou, 215123 China.
authors: Fudong
authors: F
authors: Cai
authors: Shanghai Animal Disease Control Center, Shanghai, 201103 China.
authors: Jing
authors: J
authors: Zhang
authors: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou, 215123 China.
authors: Kang
authors: K
authors: Zhao
authors: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou, 215123 China. Electronic address: denganping@suda.edu.cn.
authors: Anping
authors: A
authors: Deng
authors: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou, 215123 China. Electronic address: lijgsd@suda.edu.cn.
authors: Jianguo
authors: J
authors: Li
mesh_terms: D000814:Aniline Compounds / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004983:Ethanolamines / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation*; D008163:Luminescent Measurements / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry*; D058726:Nanoconjugates / Q000737:chemistry / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry / Q000648:ultrastructure; D011073:Polyamines / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D000814:Aniline Compounds; D004983:Ethanolamines; D058726:Nanoconjugates; C531249:Poly(amidoamine); D011073:Polyamines; C107573:brombuterol; D006046:Gold; D006108:Graphite; D015034:Zinc Oxide
keywords: Au nanoparticles; Brombuterol; CdTe QDs; ECL immunosensor; Poly(amidoamine)-graphene; ZnO nanorods