title: Longitudinal effects of developmental bisphenol A and variable diet exposures on epigenetic drift in mice.
abstract: Environmental factors, including exogenous exposures and nutritional status, can affect DNA methylation across the epigenome, but effects of exposures on age-dependent epigenetic drift remain unclear. Here, we tested the hypothesis that early-life exposure to bisphenol A (BPA) and/or variable diet results in altered epigenetic drift, as measured longitudinally via target loci methylation in paired mouse tail tissue (3 wks/10 mos old). Methylation was quantified at two repetitive elements (LINE-1, IAP), two imprinted genes (Igf2, H19), and one non-imprinted gene (Esr1) in isogenic mice developmentally exposed to Control, Control+BPA (50μg/kg diet), Mediterranean, Western, Mediterranean+BPA, or Western+BPA diets. Across age, methylation levels significantly (p<0.050) decreased at LINE-1, IAP, and H19, and increased at Esr1. Igf2 demonstrated Western-specific changes in early-life methylation (p=0.027), and IAP showed marginal negative modification of drift in Western (p=0.058) and Western+BPA (p=0.051). Thus, DNA methylation drifts across age, and developmental nutritional exposures can alter age-related methylation patterns.
authors: Environmental Health Sciences, University of Michigan, School of Public Health, 1415 Washington Heights Ann Arbor, MI, 48109, USA. Electronic address: jjkoch@umich.edu.
authors: Joseph
authors: J
authors: Kochmanski
authors: Environmental Health Sciences, University of Michigan, School of Public Health, 1415 Washington Heights Ann Arbor, MI, 48109, USA. Electronic address: marcheh@umich.edu.
authors: Elizabeth H
authors: EH
authors: Marchlewicz
authors: Environmental Health Sciences, University of Michigan, School of Public Health, 1415 Washington Heights Ann Arbor, MI, 48109, USA. Electronic address: msavidge@umich.edu.
authors: Matthew
authors: M
authors: Savidge
authors: Environmental Health Sciences, University of Michigan, School of Public Health, 1415 Washington Heights Ann Arbor, MI, 48109, USA. Electronic address: montluke@umich.edu.
authors: Luke
authors: L
authors: Montrose
authors: Environmental Health Sciences, University of Michigan, School of Public Health, 1415 Washington Heights Ann Arbor, MI, 48109, USA; Department of Animal Science, University of Minnesota, 1364 Eckles Ave, Falcon Heights, MN 55108, USA. Electronic address: cfaulk@umn.edu.
authors: Christopher
authors: C
authors: Faulk
authors: Environmental Health Sciences, University of Michigan, School of Public Health, 1415 Washington Heights Ann Arbor, MI, 48109, USA; Nutritional Sciences, University of Michigan, School of Public Health, 1415 Washington Heights Ann Arbor, MI, 48109, USA. Electronic address: ddolinoy@umich.edu.
authors: Dana C
authors: DC
authors: Dolinoy
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D019175:DNA Methylation / Q000187:drug effects*; D004032:Diet; D004785:Environmental Pollutants / Q000633:toxicity*; D044127:Epigenesis, Genetic / Q000187:drug effects*; D005260:Female; D051379:Mice; D009752:Nutritional Status; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced* / Q000235:genetics; D013997:Time Factors; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; DNA methylation; Developmental origins of health and disease; Drift; Epigenetics; High fat diet