title: Graphene Quantum Dot-MnO2 Nanosheet Based Optical Sensing Platform: A Sensitive Fluorescence "Turn Off-On" Nanosensor for Glutathione Detection and Intracellular Imaging.
abstract: Glutathione (GSH) monitoring has attracted extensive attention because it serves a vital role in human pathologies. Herein, a convenient fluorescence "turn off-on" nanosensor based on graphene quantum dots (GQDs)-manganese dioxide (MnO2) nanosheet has been designed for selective detection of GSH in living cells. The fluorescence intensity of GQDs can be quenched by MnO2 nanosheets via a fluorescence resonance energy transfer. However, GSH can reduce MnO2 nanosheets to Mn(2+) cations and release GQDs, causing sufficient recovery of fluorescent signal. The MnO2 nanosheets serve as both fluorescence nanoquencher and GSH recognizer in the sensing platform. The sensing platform displayed a sensitive response to GSH in the range of 0.5-10 μmol L(-1), with a detection limit of 150 nmol L(-1). Furthermore, the chemical response of the GQDs-MnO2 nanoprobe exhibits high selectivity toward GSH over other electrolytes and biomolecules. Most importantly, the promising platform was successfully applied in monitoring the intracellular GSH in living cells, indicating its great potential to be used in disease diagnosis. Meanwhile, this GQDs-MnO2 platform is also generalizable and can be easily expanded to the detection and imaging of other reactive species in living cells.
authors: School of Mechanical and Materials Engineering, Washington State University , Pullman, Washington 99164, United States.|Department of Analytical Chemistry, College of Chemistry, Jilin University , Changchun 130012, P. R. China.
authors: Xu
authors: X
authors: Yan
authors: Department of Analytical Chemistry, College of Chemistry, Jilin University , Changchun 130012, P. R. China.
authors: Yang
authors: Y
authors: Song
authors: Department of Analytical Chemistry, College of Chemistry, Jilin University , Changchun 130012, P. R. China.
authors: Chengzhou
authors: C
authors: Zhu
authors: Department of Analytical Chemistry, College of Chemistry, Jilin University , Changchun 130012, P. R. China.
authors: Junhua
authors: J
authors: Song
authors: School of Mechanical and Materials Engineering, Washington State University , Pullman, Washington 99164, United States.|Key Laboratory of Pesticide and Chemical Biology of the Ministry of Education, P. R. China and College of Chemistry, Central China Normal University , Wuhan 430079, P. R. China.
authors: Dan
authors: D
authors: Du
authors: Department of Analytical Chemistry, College of Chemistry, Jilin University , Changchun 130012, P. R. China.
authors: Xingguang
authors: X
authors: Su
authors: School of Mechanical and Materials Engineering, Washington State University , Pullman, Washington 99164, United States.
authors: Yuehe
authors: Y
authors: Lin
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D005978:Glutathione; D006108:Graphite; D057230:Limit of Detection; D017895:Manganese Compounds; D010087:Oxides; D045663:Quantum Dots
chemical_list: D017895:Manganese Compounds; D010087:Oxides; D006108:Graphite; D005978:Glutathione; C016552:manganese dioxide
keywords: fluorescence resonance energy transfer; glutathione; graphene quantum dots; intracellular imaging; manganese dioxide nanosheets