title: Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals.
abstract: Serological surveillance and vaccination are important strategies for controlling infectious diseases of food production animals. However, the compatibility of these strategies is limited by a lack of assays capable of differentiating infected from vaccinated animals (DIVA tests) for established killed or attenuated vaccines. Here, we used next generation phage-display (NGPD) and a 2-proportion Z score analysis to identify peptides that were preferentially bound by IgY from chickens infected with Salmonella Typhimurium or S. Enteritidis compared to IgY from vaccinates, for both an attenuated and an inactivated commercial vaccine. Peptides that were highly enriched against IgY from at least 4 out of 10 infected chickens were selected: 18 and 12 peptides for the killed and attenuated vaccines, respectively. The ten most discriminatory peptides for each vaccine were identified in an ELISA using a training set of IgY samples. These peptides were then used in multi-peptide assays that, when analysing a wider set of samples from infected and vaccinated animals, diagnosed infection with 100% sensitivity and specificity. The data describes a method for the development of DIVA assays for conventional attenuated and killed vaccines.
authors: School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.
authors: Ibrahim A
authors: IA
authors: Naqid
authors: ADAS UK, School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.
authors: Jonathan P
authors: JP
authors: Owen
authors: ADAS UK, School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.
authors: Ben C
authors: BC
authors: Maddison
authors: School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.
authors: Anastasios
authors: A
authors: Spiliotopoulos
authors: School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.|Advanced Data Analysis Centre, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.
authors: Richard D
authors: RD
authors: Emes
authors: School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.|Advanced Data Analysis Centre, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.
authors: Andrew
authors: A
authors: Warry
authors: School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.
authors: Robin J
authors: RJ
authors: Flynn
authors: Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK.
authors: Francesca
authors: F
authors: Martelli
authors: Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK.
authors: Rebecca J
authors: RJ
authors: Gosling
authors: School of Veterinary Medicine, University of Surrey, Guildford, Surrey, GU2 7AL, UK.
authors: Robert H
authors: RH
authors: Davies
authors: School of Veterinary Medicine, University of Surrey, Guildford, Surrey, GU2 7AL, UK.
authors: Roberto M
authors: RM
authors: La Ragione
authors: School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.
authors: Kevin C
authors: KC
authors: Gough
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D019779:Salmonella enterica / Q000276:immunology*
chemical_list: D001428:Bacterial Vaccines