title: Multiplex 16S rRNA-derived geno-biochip for detection of 16 bacterial pathogens from contaminated foods.
abstract: Foodborne diseases caused by various pathogenic bacteria occur worldwide. To prevent foodborne diseases and minimize their impacts, it is important to inspect contaminated foods and specifically detect many types of pathogenic bacteria. Several DNA oligonucleotide biochips based on 16S rRNA have been investigated to detect bacteria; however, a mode of detection that can be used to detect diverse pathogenic strains and to examine the safety of food matrixes is still needed. In the present work, a 16S rRNA gene-derived geno-biochip detection system was developed after screening DNA oligonucleotide specific capture probes, and it was validated for multiple detection of 16 pathogenic strains that frequently occur with a signature pattern. rRNAs were also used as detection targets directly obtained from cell lysates without any purification and amplification steps in the bacterial cells separated from 8 food matrixes by simple pretreatments. Thus, the developed 16S rRNA-derived geno-biochip can be successfully used for the rapid and multiple detection of the 16 pathogenic bacteria frequently isolated from contaminated foods that are important for food safety.
authors: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea.
authors: Hwa Hui
authors: HH
authors: Shin
authors: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea.|Division of Bioengineering, Incheon National University, Incheon, Korea.
authors: Byeong Hee
authors: BH
authors: Hwang
authors: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea.
authors: Hyung Joon
authors: HJ
authors: Cha
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D005516:Food Microbiology; D064088:Hazard Analysis and Critical Control Points; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Direct RNA-detection; Food matrix; Foodborne pathogen; Multiple detection; Oligonucleotide microarray