title: Efficacy of horse chestnut leaf extract ALH-L1005 as a matrix metalloproteinase inhibitor in ligature-induced periodontitis in canine model.
abstract: Matrix metalloproteinases (MMPs) are the main proteinases associated with periodontal tissue destruction and remodeling. Therefore, inhibition of host-derived MMPs has a key role in the prevention and reduction of periodontitis progression. Horse chestnut (Aesculus hippocastanumL.) extracts have been used as treatments for inflammatory disease, traditionally. This study assessed the clinical effect as a MMP inhibitor of horse chestnut leaf extract ALH-L1005 on periodontitis. ALH-L1005 was obtained from horse chestnut leaf and its MMP inhibitory activities estimated. Periodontitis was induced in beagles assigned to 4 groups and medicated for 6 weeks: low dose test (LT; ALH-L1005, 100 mg/kg/day), high dose test (HT; ALH-L1005, 200 mg/kg/day), positive control (PC; doxycycline, 10 mg/kg/day), or negative control (NC; placebo). Before and after administration, clinical indices of the teeth and MMP quantity in gingival tissues using zymography were measured. Clinical conditions of the LT, HT, and PC groups were significantly improved after 6 weeks. In zymographic evaluations, gelatinolytic and caseinolytic activities were suppressed in LT, HT, and PC groups but not in the NC group. The results suggest that ALH-L1005 could be an effective agent for clinical prevention and treatment of periodontitis by inhibiting the gelatinase and collagenase activities, which can detach periodontal ligaments from alveolar bone.
authors: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors: Se Eun
authors: SE
authors: Kim
authors: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors: Tae Hyun
authors: TH
authors: Kim
authors: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors: Shin Ae
authors: SA
authors: Park
authors: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors: Won Tae
authors: WT
authors: Kim
authors: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors: Young Woo
authors: YW
authors: Park
authors: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors: Jae Sang
authors: JS
authors: Ahn
authors: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors: Manbok
authors: M
authors: Jeong
authors: AngioLab, Inc. Daejeon 34016, Korea.
authors: Min-Young
authors: MY
authors: Kim
authors: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors: Kangmoon
authors: K
authors: Seo
mesh_terms: D031319:Aesculus / Q000737:chemistry; D000818:Animals; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005881:Gingiva / Q000378:metabolism / Q000601:surgery; D008026:Ligation / Q000009:adverse effects / Q000662:veterinary; D061965:Matrix Metalloproteinase Inhibitors / Q000627:therapeutic use*; D010518:Periodontitis / Q000188:drug therapy / Q000662:veterinary*; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts
keywords: dog; doxycycline; horse chestnut leaf extract; matrix metalloproteinase; periodontal disease