title: Cytoskeletal impairment during isoamyl alcohol-induced cell elongation in budding yeast.
abstract: Isoamyl alcohol (IAA) induces pseudohyphae including cell elongation in the budding yeast Saccharomyces cerevisiae. Detailed regulation of microtubules and actin in developmental transition during cell elongation is poorly understood. Here, we show that although IAA did not affect the intracellular actin level, it reduced the levels of both α- and β-tubulins. In budding yeast, cytoplasmic microtubules are linked to actin via complexes consisting of at least Kar9, Bim1, and Myo2, and reach from the spindle pole body to the cortical attachment site at the bud tip. However, IAA did not affect migration of Myo2 to the bud tip and kept Kar9 in the interior portion of the cell. In addition, bud elongation was observed in Kar9-overexpressing cells in the absence of IAA. These results indicate that impairment of the link between cytoplasmic microtubules and actin is possibly involved in the lowered interaction of Myo2 with Kar9. Our study might explain the reason for delayed cell cycle during IAA-induced cell elongation.
authors: Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.|Department of Materials Science, National Institute of Technology, Yonago College, Tottori 683-8502, Japan.
authors: Wakae
authors: W
authors: Murata
authors: Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.
authors: Satoko
authors: S
authors: Kinpara
authors: Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.
authors: Nozomi
authors: N
authors: Kitahara
authors: Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.|The OCU Advanced Research Institute for Natural Science and Technology, Osaka City University, Osaka 558-8585, Japan.
authors: Yoshihiro
authors: Y
authors: Yamaguchi
authors: Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.|Research Center for Urban Health and Sports, Osaka City University, Osaka 558-8585, Japan.
authors: Akira
authors: A
authors: Ogita
authors: Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.
authors: Toshio
authors: T
authors: Tanaka
authors: Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.
authors: Ken-Ichi
authors: K
authors: Fujita
mesh_terms: D003599:Cytoskeleton / Q000378:metabolism*; D000439:Pentanols / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
chemical_list: D000439:Pentanols; D014404:Tubulin; C029683:isopentyl alcohol