title: Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins.
abstract: Using the genome sequence of IgA1 protease of N. meningitidis of serogroup B, four recombinant proteins of different structure and molecular weight were constructed. These proteins were equal in inducing the formation of specific antibodies to IgA1 protease and had protective properties against meningococci. In the sera of immunized mice, anti-IgA1 protease antibodies were detected by whole-cell ELISA, which indicated the presence of IgA1 protease on the surface of these bacteria. We hypothesized that the protective properties of IgA1 protease-based antigens and IgA1 protease analogs could be realized not only via impairment of bacterium adhesion to the mucosa, but also via suppression of this pathogen in the organism. The presented findings seem promising for using these proteins as the basis for anti-meningococcus vaccine.
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. ovkot.2003@mail.ru.
authors: O V
authors: OV
authors: Kotelnikova
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: A A
authors: AA
authors: Zinchenko
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: A A
authors: AA
authors: Vikhrov
authors: Institute of Medicine, People's Friendship University of Russia, Moscow, Russia.
authors: A P
authors: AP
authors: Alliluev
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: O V
authors: OV
authors: Serova
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: E A
authors: EA
authors: Gordeeva
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: L S
authors: LS
authors: Zhigis
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: V S
authors: VS
authors: Zueva
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: O A
authors: OA
authors: Razgulyaeva
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: T D
authors: TD
authors: Melikhova
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: E A
authors: EA
authors: Nokel
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: E Yu
authors: EY
authors: Drozhzhina
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: L D
authors: LD
authors: Rumsh
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D009345:Neisseria meningitidis / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D011994:Recombinant Proteins; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase
keywords: IgA1 protease; immunogenicity; meningococcus; recombinant proteins