title: Efficiency of emulsifier-free emulsions and emulsions containing rapeseed lecithin as delivery systems for vectorization and release of coenzyme Q10: physico-chemical properties and in vitro evaluation.
abstract: To improve the encapsulation and release of coenzyme Q10 (CoQ10), emulsifier-free-emulsions were developed with a new emulsification process using high-frequency ultrasound (HFU) at 1.7MHz. Nano-emulsions containing CoQ10 were prepared with or without rapeseed lecithin as an emulsifier. The emulsions prepared with HFU were compared with an emulsion of CoQ10 containing emulsifier prepared with the same emulsification technique as well as with emulsions prepared with low-frequency ultrasound coupled with high-pressure homogenization (LFU+HPH). The physico-chemical properties of the emulsions were determined by average droplet size measurement with nano-droplet tracking analysis, droplet surface charge with ζ potential measurement, surface tension and rheological behaviour. Emulsions made by LFU+HPH with an emulsifier showed lower droplet sizes due to cavitation generated by the HFU process. Surface tension results showed that there was no significant difference between emulsions containing lecithin emulsifier regardless of the preparation process or the inclusion of CoQ10. In vitro biocompatibility tests were performed on human mesenchymal stem cells in order to show the cytotoxicity of various formulations and the efficiency of CoQ10-loaded emulsions. In vitro tests proved that the vectors were not toxic. Furthermore, CoQ10 facilitated a high rate of cell proliferation and metabolic activity especially when in an emulsifier-free formulation.
authors: Université de Lorraine, Laboratoire d'Ingénierie des Biomolécules (LIBio), 2 Avenue de la Forêt de Haye - TSA 40602, 54518 - Vandœuvre-lès-Nancy Cedex, France; SAS GENIALIS Route d'Achères, 18250 Henrichemont, France. Electronic address: mouna.kaci@gmail.com.
authors: M
authors: M
authors: Kaci
authors: Université de Lorraine, Laboratoire d'Ingénierie des Biomolécules (LIBio), 2 Avenue de la Forêt de Haye - TSA 40602, 54518 - Vandœuvre-lès-Nancy Cedex, France. Electronic address: elmira.arab-tehrany@univ-lorraine.fr.
authors: E
authors: E
authors: Arab-Tehrany
authors: Biopôle de l'Université de Lorraine, Campus Biologie-Santé, Faculté de Médecine, UMR 7365 CNRS-UL Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Avenue de la Forêt de Haye - C.S. 50184 - F-54505 Vandœuvre-lès-Nancy Cedex, France.
authors: G
authors: G
authors: Dostert
authors: SAS GENIALIS Route d'Achères, 18250 Henrichemont, France.
authors: I
authors: I
authors: Desjardins
authors: Biopôle de l'Université de Lorraine, Campus Biologie-Santé, Faculté de Médecine, UMR 7365 CNRS-UL Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Avenue de la Forêt de Haye - C.S. 50184 - F-54505 Vandœuvre-lès-Nancy Cedex, France.
authors: E
authors: E
authors: Velot
authors: Université de Lorraine, Laboratoire d'Ingénierie des Biomolécules (LIBio), 2 Avenue de la Forêt de Haye - TSA 40602, 54518 - Vandœuvre-lès-Nancy Cedex, France.
authors: S
authors: S
authors: Desobry
mesh_terms: D029741:Brassica rapa / Q000737:chemistry*; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D016503:Drug Delivery Systems; D045703:Emulsifying Agents / Q000737:chemistry*; D004655:Emulsions; D006801:Humans; D054709:Lecithins / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D013501:Surface-Active Agents / Q000737:chemistry; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D014465:Ultrasonics
chemical_list: D045703:Emulsifying Agents; D004655:Emulsions; D054709:Lecithins; D013501:Surface-Active Agents; D014451:Ubiquinone; C024989:coenzyme Q10
keywords: Bioavailability; Coenzyme Q(10); Emulsifier-free-nanoemulsions; Nano-emulsions; Ultrasound emulsification; Vectorization