title: A Novel Plasmid, pSx1, Harboring a New Tn1696 Derivative from Extensively Drug-Resistant Shewanella xiamenensis Encoding OXA-416.
abstract: The whole genome sequencing of extensively drug-resistant Shewanella xiamenensis T17 isolated from hospital effluents in Algeria revealed the presence of a novel 268.4 kb plasmid designated pSx1, which carries several antibiotic-resistance genes in the novel Tn1696 derivative (Tn6297), in addition to the chromosomal blaOXA-48-like gene (blaOXA-416). The presence of the plasmid was confirmed by nuclease S1-PFGE analysis and transformation by electroporation into Escherichia coli DH10B. Tn6297 contains an In27 class 1 integron harboring the dfrA12-orfF-aadA2 array, msr(E) and mph(E) associated with IS26; a new efflux pump multidrug resistance composite transposon delimited by two ISEc29s; Tn-tet harboring tetR and tetA(C); a class 1 integron with the qacG gene cassette; qnrVC6 and dfrA23 associated with ISCR1; and a complex class 1 integron In4-like containing aacC1, aadA1, blaVEB-16, catA2, sul1Δ, cmlA9, tetR, tetA(G), aac(6')-II, and blaPSE-1. Its mer operon carries merB, but lacks merC, in contrast to Tn1696 and Tn21. This study represents the first characterization of a multidrug-resistant transposon and multidrug resistance plasmid in Shewanella and is the first report of blaOXA-416in Algeria, providing evidence that Shewanella spp. could be an important reservoir and vehicle for drug resistance genes.
authors: 1 Laboratoire d'Écologie Microbienne, FSNV, Université A/MIRA de Bejaia , Bejaia, Algérie.|2 Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec , Sainte-Anne-de-Bellevue, Canada .
authors: Khadidja
authors: K
authors: Yousfi
authors: 1 Laboratoire d'Écologie Microbienne, FSNV, Université A/MIRA de Bejaia , Bejaia, Algérie.
authors: Abdelaziz
authors: A
authors: Touati
authors: 2 Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec , Sainte-Anne-de-Bellevue, Canada .
authors: Brigitte
authors: B
authors: Lefebvre
authors: 2 Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec , Sainte-Anne-de-Bellevue, Canada .
authors: Éric
authors: É
authors: Fournier
authors: 2 Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec , Sainte-Anne-de-Bellevue, Canada .
authors: Jean-Charles
authors: JC
authors: Côté
authors: 2 Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec , Sainte-Anne-de-Bellevue, Canada .
authors: Hafid
authors: H
authors: Soualhine
authors: 3 National Microbiology Laboratory, Public Health Agency of Canada , Winnipeg, Canada .
authors: Matthew
authors: M
authors: Walker
authors: 1 Laboratoire d'Écologie Microbienne, FSNV, Université A/MIRA de Bejaia , Bejaia, Algérie.
authors: Djamila
authors: D
authors: Bougdour
authors: 2 Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec , Sainte-Anne-de-Bellevue, Canada .|4 Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal , Montréal, Canada .
authors: Cécile
authors: C
authors: Tremblay
authors: 2 Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec , Sainte-Anne-de-Bellevue, Canada .|4 Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal , Montréal, Canada .
authors: Sadjia
authors: S
authors: Bekal
mesh_terms: D000462:Algeria / Q000453:epidemiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D059014:High-Throughput Nucleotide Sequencing; D006761:Hospitals; D006801:Humans; D032023:Integrons; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020592:Shewanella / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014169:Transformation, Bacterial; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D011994:Recombinant Proteins; D001618:beta-Lactamases
keywords: Shewanella xiamenensis; Tn1696 derivative; blaOXA-48; plasmid pSx1