title: In Situ Strategy to Encapsulate Antibiotics in a Bioinspired CaCO3 Structure Enabling pH-Sensitive Drug Release Apt for Therapeutic and Imaging Applications.
abstract: Herein we demonstrate a bioinspired method involving macromolecular assembly of anionic polypeptide with cationic peptide-oligomer that allows for in situ encapsulation of antibiotics like tetracycline in CaCO3 microstructure. In a single step one-pot process, the encapsulation of the drug occurs under desirable environmentally benign conditions resulting in drug loaded CaCO3 microspheres. While this tetracycline-loaded sample exhibits pH dependent in vitro drug-release profile and excellent antibacterial activity, the encapsulated drug or the dye-conjugated peptide emits fluorescence suitable for optical imaging and detection, thereby making it a multitasking material. The efficacy of tetracycline loaded calcium carbonate microspheres as pH dependent drug delivery vehicles is further substantiated by performing cell viability experiments using normal and cancer cell lines (in vitro). Interestingly, the pH-dependent drug release enables selective cytotoxicity toward cancer cell lines as compared to the normal cells, thus having the potential for further development of therapeutic applications.
authors: Nanomaterials Laboratory, Inorganic and Physical Chemistry Division, CSIR-Indian Institute of Chemical Technology , Hyderabad-500 007, India.
authors: Gousia
authors: G
authors: Begum
authors: Nanomaterials Laboratory, Inorganic and Physical Chemistry Division, CSIR-Indian Institute of Chemical Technology , Hyderabad-500 007, India.
authors: Thuniki Naveen
authors: TN
authors: Reddy
authors: Toxicology Unit, Biology Division, CSIR-Indian Institute of Chemical Technology , Hyderabad-500 007, India.
authors: K Pranay
authors: KP
authors: Kumar
authors: Toxicology Unit, Biology Division, CSIR-Indian Institute of Chemical Technology , Hyderabad-500 007, India.
authors: Koude
authors: K
authors: Dhevendar
authors: CSIR-Centre for Cellular and Molecular Biology , Hyderabad-500 007, India.
authors: Shashi
authors: S
authors: Singh
authors: CSIR-Centre for Cellular and Molecular Biology , Hyderabad-500 007, India.
authors: Miriyala
authors: M
authors: Amarnath
authors: Toxicology Unit, Biology Division, CSIR-Indian Institute of Chemical Technology , Hyderabad-500 007, India.
authors: Sunil
authors: S
authors: Misra
authors: Department of Materials Science and Engineering, Tuskegee University , Tuskegee, Alabama 36088, United States.
authors: Vijaya K
authors: VK
authors: Rangari
authors: Nanomaterials Laboratory, Inorganic and Physical Chemistry Division, CSIR-Indian Institute of Chemical Technology , Hyderabad-500 007, India.
authors: Rohit Kumar
authors: RK
authors: Rana
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002119:Calcium Carbonate; D016503:Drug Delivery Systems; D065546:Drug Liberation; D006863:Hydrogen-Ion Concentration; D008863:Microspheres
chemical_list: D000900:Anti-Bacterial Agents; D002119:Calcium Carbonate
keywords: antibacterial; antibiotic; anticancer; bioinspired; calcium carbonate; fluorescence