title: Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye.
abstract: Human corneal epithelial (HCE-T) and human lens epithelial (SRA01/04) cells derived from the human eye were exposed to 60 gigahertz (GHz) millimeter-wavelength radiation for 24 h. There was no statistically significant increase in the micronucleus (MN) frequency in cells exposed to 60 GHz millimeter-wavelength radiation at 1 mW/cm² compared with sham-exposed controls and incubator controls. The MN frequency of cells treated with bleomycin for 1 h provided positive controls. The comet assay, used to detect DNA strand breaks, and heat shock protein (Hsp) expression also showed no statistically significant effects of exposure. These results indicate that exposure to millimeter-wavelength radiation has no effect on genotoxicity in human eye cells.
authors: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. shin_koyama@rish.kyoto-u.ac.jp.
authors: Shin
authors: S
authors: Koyama
authors: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. narita.eijirou.4e@kyoto-u.ac.jp.
authors: Eijiro
authors: E
authors: Narita
authors: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. yoko_shimizu@rish.kyoto-u.ac.jp.
authors: Yoko
authors: Y
authors: Shimizu
authors: Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. y_suzuki@tmu.ac.jp.
authors: Yukihisa
authors: Y
authors: Suzuki
authors: Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. shiina-takeo@ed.tmu.ac.jp.
authors: Takeo
authors: T
authors: Shiina
authors: Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. masao@tmu.ac.jp.
authors: Masao
authors: M
authors: Taki
authors: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. shino@rish.kyoto-u.ac.jp.
authors: Naoki
authors: N
authors: Shinohara
authors: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. miyakoshi@rish.kyoto-u.ac.jp.
authors: Junji
authors: J
authors: Miyakoshi
mesh_terms: D001761:Bleomycin; D002460:Cell Line / Q000528:radiation effects*; D020552:Comet Assay; D004249:DNA Damage / Q000528:radiation effects*; D005123:Eye; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007908:Lens, Crystalline; D008872:Microwaves; D000069079:Radiation Exposure / Q000009:adverse effects*; D011846:Radio Waves / Q000009:adverse effects*
chemical_list: D006360:Heat-Shock Proteins; D001761:Bleomycin
keywords: cellular genotoxicity; comet assay; heat shock protein; human eye cells; long-term exposure; micronucleus formation; millimeter-waves