title: Changes in total plasma and serum N-glycome composition and patient-controlled analgesia after major abdominal surgery.
abstract: Systemic inflammation participates to the complex healing process occurring after major surgery, thus directly affecting the surgical outcome and patient recovery. Total plasma N-glycome might be an indicator of inflammation after major surgery, as well as an anti-inflammatory therapy response marker, since protein glycosylation plays an essential role in the inflammatory cascade. Therefore, we assessed the effects of surgery on the total plasma N-glycome and the association with self-administration of postoperative morphine in two cohorts of patients that underwent major abdominal surgery. We found that plasma N-glycome undergoes significant changes one day after surgery and intensifies one day later, thus indicating a systemic physiological response. In particular, we observed the increase of bisialylated biantennary glycan, A2G2S[3,6]2, 12 hours after surgery, which progressively increased until 48 postoperative hours. Most changes occurred 24 hours after surgery with the decrease of most core-fucosylated biantennary structures, as well as the increase in sialylated tetraantennary and FA3G3S[3,3,3]3 structures. Moreover, we observed a progressive increase of sialylated triantennary and tetraantennary structures two days after surgery, with a concomitant decrease of the structures containing bisecting N-acetylglucosamine along with bi- and trisialylated triantennary glycans. We did not find any statistically significant association between morphine consumption and plasma N-glycome.
authors: Genos Glycoscience Research Laboratory, Zagreb, Croatia.
authors: Ivan
authors: I
authors: Gudelj
authors: Department of Anesthesia, ICU and Pain Therapy, University Hospital of Parma, Parma, Italy.|SIMPAR Group (Study in Multidisciplinary Pain Research), Parma, Italy.|Department of Surgical Sciences, University of Parma, Parma, Italy.
authors: Marco
authors: M
authors: Baciarello
authors: Genos Glycoscience Research Laboratory, Zagreb, Croatia.|University of Zagreb Faculty of Pharmacy and Biochemistry, Zagreb, Croatia.
authors: Ivo
authors: I
authors: Ugrina
authors: SIMPAR Group (Study in Multidisciplinary Pain Research), Parma, Italy.|Pain Therapy Service, Fondazione IRCCS Policlinico S. Matteo, Pavia, Italy.|YAP (Young Against Pain) group, Parma, Italy.
authors: Manuela
authors: M
authors: De Gregori
authors: SIMPAR Group (Study in Multidisciplinary Pain Research), Parma, Italy.|Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Palo Alto, CA, USA.
authors: Valerio
authors: V
authors: Napolioni
authors: SIMPAR Group (Study in Multidisciplinary Pain Research), Parma, Italy.|Department of Anesthesia, Montreal Children's Hospital, Canada.
authors: Pablo M
authors: PM
authors: Ingelmo
authors: Department of Anesthesia, ICU and Pain Therapy, University Hospital of Parma, Parma, Italy.|SIMPAR Group (Study in Multidisciplinary Pain Research), Parma, Italy.|Department of Surgical Sciences, University of Parma, Parma, Italy.
authors: Dario
authors: D
authors: Bugada
authors: Clinical and Experimental Pharmacokinetics Unit, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
authors: Simona
authors: S
authors: De Gregori
authors: Department of Medical Biochemistry and Laboratory Medicine, Clinical Hospital Merkur, Zagreb, Croatia.
authors: Lovorka
authors: L
authors: Đerek
authors: Genos Glycoscience Research Laboratory, Zagreb, Croatia.
authors: Maja
authors: M
authors: Pučić-Baković
authors: Genos Glycoscience Research Laboratory, Zagreb, Croatia.
authors: Mislav
authors: M
authors: Novokmet
authors: University of Zagreb Faculty of Pharmacy and Biochemistry, Zagreb, Croatia.
authors: Olga
authors: O
authors: Gornik
authors: Department of Biomedical, Biotechnological and Translational Science (S.Bi.Bi.T.), University of Parma, Parma, Italy.
authors: Gloria
authors: G
authors: Saccani Jotti
authors: Department of Clinical and Experimental Medicine, University of Parma, Italy.
authors: Tiziana
authors: T
authors: Meschi
authors: Genos Glycoscience Research Laboratory, Zagreb, Croatia.|University of Zagreb Faculty of Pharmacy and Biochemistry, Zagreb, Croatia.
authors: Gordan
authors: G
authors: Lauc
authors: Department of Anesthesia, ICU and Pain Therapy, University Hospital of Parma, Parma, Italy.|SIMPAR Group (Study in Multidisciplinary Pain Research), Parma, Italy.|Department of Surgical Sciences, University of Parma, Parma, Italy.
authors: Massimo
authors: M
authors: Allegri
mesh_terms: D000005:Abdomen / Q000601:surgery*; D000328:Adult; D000368:Aged; D016058:Analgesia, Patient-Controlled; D001798:Blood Proteins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D015331:Cohort Studies; D005260:Female; D005643:Fucose / Q000737:chemistry; D054794:Glycomics; D006031:Glycosylation; D006801:Humans; D007249:Inflammation; D008297:Male; D008875:Middle Aged; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry; D044967:Serum / Q000737:chemistry*; D012794:Sialic Acids / Q000737:chemistry; D013514:Surgical Procedures, Operative; D055815:Young Adult
chemical_list: D001798:Blood Proteins; D011134:Polysaccharides; D012794:Sialic Acids; D005643:Fucose