title: Comparative Transcriptional Profiling of Primed and Non-primed Rice Seedlings under Submergence Stress.
abstract: Submergence stress is a limiting factor for direct-seeded rice systems in rainfed lowlands and flood-prone areas of South and Southeast Asia. The present study demonstrated that submergence stress severely hampered the germination and seedling growth of rice, however, seed priming alleviated the detrimental effects of submergence stress. To elucidate the molecular basis of seed priming-induced submergence tolerance, transcriptome analyses were performed using 4-day-old primed (selenium-Se and salicylic acid-SA priming) and non-primed rice seedlings under submergence stress. Genomewide transcriptomic profiling identified 2371 and 2405 transcripts with Se- and SA-priming, respectively, that were differentially expressed in rice compared with non-priming treatment under submergence. Pathway and gene ontology term enrichment analyses revealed that genes involved in regulation of secondary metabolism, development, cell, transport, protein, and metal handling were over-represented after Se- or SA-priming. These coordinated factors might have enhanced the submergence tolerance and maintained the better germination and vigorous seedling growth of primed rice seedlings. It was also found that many genes involved in cellular and metabolic processes such as carbohydrate metabolism, cellular, and metabolic biosynthesis, nitrogen compound metabolic process, transcription, and response to oxidative stress were induced and overlapped in seed priming treatments, a finding which reveals the common mechanism of seed priming-induced submergence tolerance. Taken together, these results may provide new avenues for understanding and advancing priming-induced responses to submergence tolerance in crop plants.
authors: National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural UniversityWuhan, China; College of Resources and Environment, Huazhong Agricultural UniversityWuhan, China.
authors: Saddam
authors: S
authors: Hussain
authors: Shanghai Biotechnology Corporation Shanghai, China.
authors: Hanqi
authors: H
authors: Yin
authors: National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University Wuhan, China.
authors: Shaobing
authors: S
authors: Peng
authors: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University Wuhan, China.
authors: Faheem A
authors: FA
authors: Khan
authors: National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural UniversityWuhan, China; College of Resources and Environment, Huazhong Agricultural UniversityWuhan, China.
authors: Fahad
authors: F
authors: Khan
authors: Faculty of Agriculture and Natural Sciences, Abant Izzet Baysal University Bolu, Turkey.
authors: Muhammad
authors: M
authors: Sameeullah
authors: Department of Agronomy, University of Agriculture Faisalabad, Pakistan.
authors: Hafiz A
authors: HA
authors: Hussain
authors: National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University Wuhan, China.
authors: Jianliang
authors: J
authors: Huang
authors: National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University Wuhan, China.
authors: Kehui
authors: K
authors: Cui
authors: National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University Wuhan, China.
authors: Lixiao
authors: L
authors: Nie
keywords: direct-seeded rice; germination; seed priming; submergence stress; transcriptome analysis