title: Antibacterial Activity of Cinnamaldehyde and Estragole Extracted from Plant Essential Oils against Pseudomonas syringae pv. actinidiae Causing Bacterial Canker Disease in Kiwifruit.
abstract: Pseudomonas syringae pv. actinidiae (Psa) causes bacterial canker disease in kiwifruit. Antibacterial activity of plant essential oils (PEOs) originating from 49 plant species were tested against Psa by a vapor diffusion and a liquid culture assays. The five PEOs from Pimenta racemosa, P. dioica, Melaleuca linariifolia, M. cajuputii, and Cinnamomum cassia efficiently inhibited Psa growth by either assays. Among their major components, estragole, eugenol, and methyl eugenol showed significant antibacterial activity by only the liquid culture assay, while cinnamaldehyde exhibited antibacterial activity by both assays. The minimum inhibitory concentrations (MICs) of estragole and cinnamaldehyde by the liquid culture assay were 1,250 and 2,500 ppm, respectively. The MIC of cinnamaldehyde by the vapor diffusion assay was 5,000 ppm. Based on the formation of clear zones or the decrease of optical density caused by these compounds, they might kill the bacterial cells and this feature might be useful for managing the bacterial canker disease in kiwifruit.
authors: Department of Horticultural Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University, Yongin 17104, Korea.
authors: Yu-Rim
authors: YR
authors: Song
authors: Department of Horticultural Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University, Yongin 17104, Korea.
authors: Min-Seon
authors: MS
authors: Choi
authors: Department of Horticultural Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University, Yongin 17104, Korea.
authors: Geun-Won
authors: GW
authors: Choi
authors: Department of Forest Sciences, Seoul National University, Seoul 08826, Korea.
authors: Il-Kwon
authors: IK
authors: Park
authors: Department of Horticultural Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University, Yongin 17104, Korea.
authors: Chang-Sik
authors: CS
authors: Oh
keywords: Pseudomonas syringae pv. actinidiae; cinnamaldehyde; estragole; liquid culture assay; vapor diffusion assay