title: Protective Effect of ALA in Crushed Optic Nerve Cat Retinal Ganglion Cells Using a New Marker RBPMS.
abstract: In this study we first sought to determine whether RNA-binding protein with multiple splicing (RBPMS) can serve as a specific marker for cat retina ganglion cells (RGCs) using retrograde labeling and immunohistochemistry staining. RBPM was then used as an RGC marker to study RGC survival after optic nerve crush (ONC) and alpha-lipoic acid (ALA) treatment in cats. ALA treatment yielded a peak density of RBPMS-alpha cells within the peak isodensity zone (>60/mm2) which did not differ from ONC retinas. The area within the zone was significantly enlarged (control: 2.3%, ONC: 0.06%, ONC+ALA: 0.1%). As for the 10-21/mm2 zone, ALA treatment resulted in a significant increase in area (control: 34.5%, ONC: 12.1%, ONC+ALA: 35.9%). ALA can alleviate crush-induced RGC injury.
authors: Department of Anatomy, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory on Machine Perception (Ministry of Education), Peking University, Beijing, China.|Key Laboratory for Visual Impairment and Restoration (Ministry of Education), Peking University, Beijing, China.
authors: Yanling
authors: Y
authors: Wang
authors: Department of Anatomy, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory on Machine Perception (Ministry of Education), Peking University, Beijing, China.|Key Laboratory for Visual Impairment and Restoration (Ministry of Education), Peking University, Beijing, China.
authors: Wenyao
authors: W
authors: Wang
authors: Department of Neurobiology and Molecular, Cellular and Integrative Physiology, UCLA, Los Angeles, California, United States of America.|Jules Stein Eye Institute, UCLA, Los Angeles, California, United States of America.|UCLA College of Life Sciences, University of California, Los Angeles, CA, United States of America.
authors: Jessica
authors: J
authors: Liu
authors: Department of Anatomy, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory on Machine Perception (Ministry of Education), Peking University, Beijing, China.|Key Laboratory for Visual Impairment and Restoration (Ministry of Education), Peking University, Beijing, China.
authors: Xin
authors: X
authors: Huang
authors: Department of Anatomy, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory on Machine Perception (Ministry of Education), Peking University, Beijing, China.|Key Laboratory for Visual Impairment and Restoration (Ministry of Education), Peking University, Beijing, China.
authors: Ruixing
authors: R
authors: Liu
authors: Department of Anatomy, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory on Machine Perception (Ministry of Education), Peking University, Beijing, China.|Key Laboratory for Visual Impairment and Restoration (Ministry of Education), Peking University, Beijing, China.
authors: Huika
authors: H
authors: Xia
authors: Department of Neurobiology and Molecular, Cellular and Integrative Physiology, UCLA, Los Angeles, California, United States of America.|Jules Stein Eye Institute, UCLA, Los Angeles, California, United States of America.|UCLA College of Life Sciences, University of California, Los Angeles, CA, United States of America.
authors: Nicholas C
authors: NC
authors: Brecha
authors: Department of Anatomy, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory on Machine Perception (Ministry of Education), Peking University, Beijing, China.|Key Laboratory for Visual Impairment and Restoration (Ministry of Education), Peking University, Beijing, China.
authors: Mingliang
authors: M
authors: Pu
authors: Department of Anatomy, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory on Machine Perception (Ministry of Education), Peking University, Beijing, China.|Key Laboratory for Visual Impairment and Restoration (Ministry of Education), Peking University, Beijing, China.
authors: Jie
authors: J
authors: Gao
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002415:Cats; D019610:Cytoprotection / Q000187:drug effects*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D009409:Nerve Crush; D009900:Optic Nerve / Q000166:cytology* / Q000601:surgery*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008063:Thioctic Acid / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D016601:RNA-Binding Proteins; D008063:Thioctic Acid