title: Fast pesticide detection inside microfluidic device with integrated optical pH, oxygen sensors and algal fluorescence.
abstract: The necessities of developing fast, portable, cheap and easy to handle pesticide detection platforms are getting attention of scientific and industrial communities. Although there are some approaches to develop microchip based pesticide detection platforms, there is no compact microfluidic device for the complementary, fast, cheap, reusable and reliable analysis of different pesticides. In this work, a microfluidic device is developed for in-situ analysis of pesticide concentration detected via metabolism/photosynthesis of Chlamydomonas reinhardtii algal cells (algae) in tap water. Algae are grown in glass based microfluidic chip, which contains integrated optical pH and oxygen sensors in a portable system for on-site detection. In addition, intrinsic algal fluorescence is detected to analyze the pesticide concentration in parallel to pH and oxygen sensors with integrated fluorescence detectors. The response of the algae under the effect of different concentrations of pesticides is evaluated and complementary inhibition effects depending on the pesticide concentration are demonstrated. The three different sensors allow the determination of various pesticide concentrations in the nanomolar concentration range. The miniaturized system provides the fast quantification of pesticides in less than 10min and enables the study of toxic effects of different pesticides on Chlamydomonas reinhardtii green algae. Consequently, the microfluidic device described here provides fast and complementary detection of different pesticides with algae in a novel glass based microfluidic device with integrated optical pH, oxygen sensors and algal fluorescence.
authors: Micronit GmbH, Konrad-Adenauer-Allee 11, Dortmund, 44263 Germany. Electronic address: Bogachan.Tahirbegi@micronit.de.
authors: Islam Bogachan
authors: IB
authors: Tahirbegi
authors: Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Graz, Austria.
authors: Josef
authors: J
authors: Ehgartner
authors: Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Graz, Austria.
authors: Philipp
authors: P
authors: Sulzer
authors: Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Graz, Austria.
authors: Silvia
authors: S
authors: Zieger
authors: Micronit GmbH, Konrad-Adenauer-Allee 11, Dortmund, 44263 Germany.
authors: Alice
authors: A
authors: Kasjanow
authors: Micronit GmbH, Konrad-Adenauer-Allee 11, Dortmund, 44263 Germany.
authors: Mirco
authors: M
authors: Paradiso
authors: Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Graz, Austria.
authors: Martin
authors: M
authors: Strobl
authors: Micronit GmbH, Konrad-Adenauer-Allee 11, Dortmund, 44263 Germany.
authors: Dominique
authors: D
authors: Bouwes
authors: Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Graz, Austria.
authors: Torsten
authors: T
authors: Mayr
mesh_terms: D001280:Atrazine / Q000032:analysis / Q000633:toxicity; D015374:Biosensing Techniques / Q000295:instrumentation*; D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000502:physiology; D000460:Chlorophyta / Q000187:drug effects / Q000502:physiology; D004237:Diuron / Q000032:analysis / Q000633:toxicity; D060766:Drinking Water / Q000032:analysis; D004867:Equipment Design; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism; D010575:Pesticides / Q000032:analysis* / Q000633:toxicity; D010788:Photosynthesis / Q000187:drug effects; D012839:Simazine / Q000032:analysis / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
chemical_list: D060766:Drinking Water; D010575:Pesticides; D014874:Water Pollutants, Chemical; D004237:Diuron; D001280:Atrazine; D010100:Oxygen; D012839:Simazine
keywords: Algal metabolism; Integration of optical elements; Microfluidics; Optical sensors; Pesticide detection; Smart devices