title: Molecular characterization of genotype XIIIb Newcastle disease virus from central India during 2006-2012: Evidence of its panzootic potential.
abstract: Newcastle disease virus (NDV) is the causative agent of Newcastle disease (ND) in many avian species. ND is a serious problem in developing countries, causing huge loss in the poultry industry. Although there are reports of continuous outbreaks of ND leading to serious losses to the poultry farming, very less is known about the genetic characteristics of its strains circulating in different parts of India. In the present study, we have five isolates of NDV reported from different outbreaks in Central India between the years 2006-2012. Deduced amino acid sequence of the F protein cleavage site and phylogenetic analysis of all the five isolates showed circulation of NDV genotype XIIIb. All the isolates showed a unique virulent cleavage site112RRQKR↓F117. The close genetic similarity of all the isolates suggested circulation of the virulent NDV strains of the same ancestor in and around central India. Continuous isolation of genotype XIIIb NDV strains within the country suggests its panzootic potential. The study will be useful to understand the circulating strains of NDV and plan a vaccination strategy for poultry in India.
authors: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
authors: Sudhir
authors: S
authors: Morla
authors: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
authors: Manisha
authors: M
authors: Shah
authors: Department of Pathology, Nagpur Veterinary College, Maharashtra Animal & Fishery Sciences University, Nagpur 440 006, India.
authors: Megha
authors: M
authors: Kaore
authors: Department of Pathology, Nagpur Veterinary College, Maharashtra Animal & Fishery Sciences University, Nagpur 440 006, India.
authors: Nitin Vasantrao
authors: NV
authors: Kurkure
authors: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India. Electronic address: sachinku@iitg.ernet.in.
authors: Sachin
authors: S
authors: Kumar
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004196:Disease Outbreaks; D005838:Genotype; D007194:India / Q000453:epidemiology; D017720:Molecular Epidemiology; D009521:Newcastle Disease / Q000453:epidemiology* / Q000821:virology; D009522:Newcastle disease virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D011200:Poultry; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014760:Viral Fusion Proteins / Q000235:genetics; D015047:Zoonoses / Q000453:epidemiology* / Q000821:virology
chemical_list: D014760:Viral Fusion Proteins
keywords: Newcastle disease virus; Outbreak; Pathogenicity; Virulent