title: Nanostructured Aptamer-Functionalized Black Phosphorus Sensing Platform for Label-Free Detection of Myoglobin, a Cardiovascular Disease Biomarker.
abstract: We report the electrochemical detection of the redox active cardiac biomarker myoglobin (Mb) using aptamer-functionalized black phosphorus nanostructured electrodes by measuring direct electron transfer. The as-synthesized few-layer black phosphorus nanosheets have been functionalized with poly-l-lysine (PLL) to facilitate binding with generated anti-Mb DNA aptamers on nanostructured electrodes. This aptasensor platform has a record-low detection limit (∼0.524 pg mL(-1)) and sensitivity (36 μA pg(-1) mL cm(-2)) toward Mb with a dynamic response range from 1 pg mL(-1) to 16 μg mL(-1) for Mb in serum samples. This strategy opens up avenues to bedside technologies for multiplexed diagnosis of cardiovascular diseases in complex human samples.
authors: Institute of Nano Science & Technology , Habitat Centre, Sector-64, Mohali 160062, Punjab, India.
authors: Vinod
authors: V
authors: Kumar
authors: School of Materials, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Jack R
authors: JR
authors: Brent
authors: Institute of Nano Science & Technology , Habitat Centre, Sector-64, Mohali 160062, Punjab, India.
authors: Munish
authors: M
authors: Shorie
authors: Institute of Nano Science & Technology , Habitat Centre, Sector-64, Mohali 160062, Punjab, India.
authors: Harmanjit
authors: H
authors: Kaur
authors: Institute of Nano Science & Technology , Habitat Centre, Sector-64, Mohali 160062, Punjab, India.
authors: Gaganpreet
authors: G
authors: Chadha
authors: School of Materials, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Andrew G
authors: AG
authors: Thomas
authors: School of Materials, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Edward A
authors: EA
authors: Lewis
authors: School of Materials, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Aidan P
authors: AP
authors: Rooney
authors: School of Materials, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Lan
authors: L
authors: Nguyen
authors: School of Materials, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Xiang Li
authors: XL
authors: Zhong
authors: School of Materials, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: M Grace
authors: MG
authors: Burke
authors: School of Materials, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Sarah J
authors: SJ
authors: Haigh
authors: School of Chemistry, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Alex
authors: A
authors: Walton
authors: School of Chemistry, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Paul D
authors: PD
authors: McNaughter
authors: School of Chemistry, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Aleksander A
authors: AA
authors: Tedstone
authors: School of Chemistry, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Nicky
authors: N
authors: Savjani
authors: School of Chemistry, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Christopher A
authors: CA
authors: Muryn
authors: School of Materials, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.|School of Chemistry, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: Paul
authors: P
authors: O'Brien
authors: Institute of Nano Science & Technology , Habitat Centre, Sector-64, Mohali 160062, Punjab, India.|Department of Chemistry, Indian Institute of Technology Delhi , Hauz Khas, New Delhi 110016, India.
authors: Ashok K
authors: AK
authors: Ganguli
authors: School of Materials, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.|School of Chemistry, University of Manchester , Oxford Road, Manchester M13 9PL, United Kingdom.
authors: David J
authors: DJ
authors: Lewis
authors: Institute of Nano Science & Technology , Habitat Centre, Sector-64, Mohali 160062, Punjab, India.
authors: Priyanka
authors: P
authors: Sabherwal
mesh_terms: D052157:Aptamers, Nucleotide; D015415:Biomarkers; D015374:Biosensing Techniques; D002318:Cardiovascular Diseases; D055664:Electrochemical Techniques; D004566:Electrodes; D006801:Humans; D009211:Myoglobin; D049329:Nanostructures; D010758:Phosphorus
chemical_list: D052157:Aptamers, Nucleotide; D015415:Biomarkers; D009211:Myoglobin; D010758:Phosphorus
keywords: 2-D materials; aptamer; black phosphorus; heart disease; myoglobin; sensing