title: Removal Characteristics of Immunoadsorption With the Immusorba TR-350 Column Using Conventional and Selective Plasma Separators.
abstract: In Japan, immunoadsorption (IA) is performed using a conventional plasma separator and Immusorba TR-350 column (TR-350) for the treatment of neurological immune diseases. By this method, TR-350 has the limited maximal capacity of the immunoglobulin G (IgG) adsorption, and fibrinogen (Fbg) is reduced remarkably. Evacure EC-4A10 (EC-4A) is a selective plasma separator and the sieving coefficients of IgG and Fbg using EC-4A were 0.5 and 0, respectively. Here, we investigated the removal characteristics of IgG and Fbg in IA by TR-350 using two different plasma membrane separators: conventional plasma separator (PE-IA) and EC-4A (EC-IA). In vitro filtration using plasma effluent was performed with a closed circuit. When the processed volume was 3 L, estimated removal amounts by PE-IA were 3172 mg for IgG and 3329 mg for Fbg, respectively. When the processed volume was 3 L, estimated removal amounts by EC-IA were 4946 mg and 1916 mg, respectively. EC-IA can be considered useful for the removal of IgG, including auto-antibodies, while retaining Fbg, thereby allowing even daily use.
authors: Medical Engineering Center, Medical Hospital of Tokyo Medical and Dental University, Tokyo, Japan.
authors: Atsushi
authors: A
authors: Ohkubo
authors: Department of Nephrology, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Tomokazu
authors: T
authors: Okado
authors: Medical Engineering Center, Medical Hospital of Tokyo Medical and Dental University, Tokyo, Japan.
authors: Satoko
authors: S
authors: Miyamoto
authors: Medical Engineering Center, Medical Hospital of Tokyo Medical and Dental University, Tokyo, Japan.
authors: Keigo
authors: K
authors: Goto
authors: Medical Engineering Center, Medical Hospital of Tokyo Medical and Dental University, Tokyo, Japan.
authors: Motoki
authors: M
authors: Yamamoto
authors: Medical Engineering Center, Medical Hospital of Tokyo Medical and Dental University, Tokyo, Japan.
authors: Takuma
authors: T
authors: Maeda
authors: Medical Engineering Center, Medical Hospital of Tokyo Medical and Dental University, Tokyo, Japan.
authors: Ayako
authors: A
authors: Itagaki
authors: Medical Engineering Center, Medical Hospital of Tokyo Medical and Dental University, Tokyo, Japan.
authors: Hiroshi
authors: H
authors: Seshima
authors: Medical Engineering Center, Medical Hospital of Tokyo Medical and Dental University, Tokyo, Japan.
authors: Naoki
authors: N
authors: Kurashima
authors: Department of Nephrology, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Eisei
authors: E
authors: Sohara
authors: Department of Nephrology, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Shinichi
authors: S
authors: Uchida
authors: Department of Nephrology, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Tatemitsu
authors: T
authors: Rai
mesh_terms: D006801:Humans; D007163:Immunosorbent Techniques / Q000295:instrumentation*; D066298:In Vitro Techniques; D010951:Plasma Exchange / Q000295:instrumentation* / Q000379:methods*
keywords: Conventional plasma separator; Immunoadsorption; Immunoglobulin G subclasses; Removal characteristics; Selective plasma separator