title: Antimicrobial activity of submicron glass fibres incorporated as a filler to a dental sealer.
abstract: Two types of antimicrobial glass fibers containing ZnO and CaO, with diameters ranging from tens of nanometers to 1 µm, were successfully fabricated by a laser spinning technique. The antimicrobial performance was corroborated according to ISO 20743:2013, by using gram-negative (Escherichia coli) and gram-positive (Streptococcus oralis, Streptococcus mutans and Staphylococcus aureus) bacteria, and yeast (Candida krusei) (more than 3 logs of reduction). The metabolic activity and endosomal system of eukaryotic cells were not altered by using eluents of CaO glass submicrometric fibers and ZnO fibers at 1 : 10 dilution as cellular media (viability rates over 70%). A dental material was functionalized by embedding ZnO nanofibers above the percolation threshold (20% wt), creating a three-dimensional (3D) fiber network that added an antimicrobial profile. This new ZnO glass fiber composite is proved non-cytotoxic and preserved the antimicrobial effect after immersion in human saliva. This is the first time that a fiber-reinforced liner with strong antimicrobial-activity has been created to prevent secondary caries. The potential of developing new fiber-reinforced composites (FRCs) with antimicrobial properties opens up an extensive field of dental applications where most important diseases have an infectious origin.
authors: School of Chemistry-CRANN, Trinity College Dublin, Green College, Dublin 2, Ireland. Department of Biomaterials and Bioinspired Materials, Materials Science Institute of Madrid, (ICMM), Spanish National Research Council (CSIC), Cantoblanco, Madrid 28049, Spain.
authors: L
authors: L
authors: Esteban-Tejeda
authors: B
authors: B
authors: Cabal
authors: R
authors: R
authors: Torrecillas
authors: C
authors: C
authors: Prado
authors: E
authors: E
authors: Fernandez-Garcia
authors: R
authors: R
authors: López-Piriz
authors: F
authors: F
authors: Quintero
authors: J
authors: J
authors: Pou
authors: J
authors: J
authors: Penide
authors: J S
authors: JS
authors: Moya
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry; D002175:Candida; D003188:Composite Resins; D003764:Dental Materials / Q000737:chemistry*; D003799:Dental Stress Analysis; D004926:Escherichia coli; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D008826:Microbial Sensitivity Tests; D041681:NIH 3T3 Cells; D010087:Oxides / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry / Q000187:drug effects; D013211:Staphylococcus aureus; D013295:Streptococcus mutans; D019533:Streptococcus oralis; D015032:Zinc / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D017610:Calcium Compounds; D003188:Composite Resins; D003764:Dental Materials; C465120:Dental Seal; D008689:Methacrylates; D010087:Oxides; D019279:Resin Cements; C009753:fiberglass; C016538:lime; D015032:Zinc; D015034:Zinc Oxide