title: Anti-adipogenic effect of Glycoside St-E2 and Glycoside St-C1 isolated from the leaves of Acanthopanax henryi (Oliv.) Harms in 3T3-L1 cells.
abstract: Acanthopanax henryi (Oliv.) Harms has been used in the treatment of arthritis, rheumatism, and abdominal pain. This study evaluated whether natural compounds isolated from the leaves of A. henryi (Oliv.) Harms could inhibit adipocyte differentiation by regulating transcriptional factors such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα). AMP-activated protein kinase (AMPK) activity was also evaluated. Among the several compounds isolated from the leaves of A. henryi (Oliv.) Harms, Glycoside St-C1 and Glycoside St-E2 significantly decreased lipid accumulation and the expressions of PPARγ and C/EBPα. Glycoside St-C1 and Glycoside St-E2 were found to activate AMPK when they regulated PPARγ and C/EBPα. Results confirmed that Glycoside St-C1 and Glycoside St-E2 isolated from the leaves of A. henryi (Oliv.) Harms can inhibit adipogenesis through the AMPK-PPARγ-C/EBPα mechanism. Thus, this study suggests that Glycoside St-C1 and Glycoside St-E2 have a therapeutic effect due to activation of the AMPKα.
authors: a Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute , Wonkwang University , Iksan , Republic of Korea.
authors: Yo-Han
authors: YH
authors: Han
authors: b School of Pharmacy , Hunan University of Chinese Medicine , Changsha , China.
authors: Zhi
authors: Z
authors: Li
authors: c College of Korean Medicine, Institute of Korean Medicine , Kyung Hee University , Seoul , Republic of Korea.
authors: Jae-Young
authors: JY
authors: Um
authors: b School of Pharmacy , Hunan University of Chinese Medicine , Changsha , China.
authors: Xiang Qian
authors: XQ
authors: Liu
authors: a Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute , Wonkwang University , Iksan , Republic of Korea.
authors: Seung-Heon
authors: SH
authors: Hong
mesh_terms: D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D028001:Eleutherococcus / Q000737:chemistry*; D006027:Glycosides / Q000302:isolation & purification* / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D006027:Glycosides; D047495:PPAR gamma; D055372:AMP-Activated Protein Kinases
keywords: AMPKα; Acanthopanax henryi (Oliv.) Harms; C/EBPα; PPARγ; adipogenesis