title: Revisiting the Male-Produced Aggregation Pheromone of the Lesser Mealworm, Alphitobius diaperinus (Coleoptera, Tenebrionidae): Identification of a Six-Component Pheromone from a Brazilian Population.
abstract: The lesser mealworm, Alphitobius diaperinus Panzer 1797 (Coleoptera: Tenebrionidae), is a cosmopolitan insect pest affecting poultry production. Due to its cryptic behavior, insecticide control is usually not efficient. Thus, sustainable and effective methods would have an enormous and positive impact in poultry production. The aim of this study was to confirm the identity of the male-produced aggregation pheromone for a Brazilian population of A. diaperinus and to evaluate its biological activity in behavioral assays. Six male-specific compounds were identified: (R)-limonene (1), (E)-ocimene (2), 2-nonanone (3), (S)-linalool (4), (R)-daucene (5), all described before in an American population, and a sixth component, (E,E)-α-farnesene (6), which is apparently exclusive to a Brazilian population. Y-Tube bioassays confirmed the presence of a male-produced aggregation pheromone and showed that all components need to be present in a similar ratio and concentration as emitted by male A. diaperinus to produce a positive chemotactic response.
authors: UnB - Universidade de Brası́lia , Departamento de Zoologia, Campus Universitário Darcy Ribeiro, Brası́lia, DF 70910-900, Brazil.|Universidade Federal do Rio Grande do Sul , PPG-Fitotecnia, Avenida Bento Gonçalves, 7712 Porto Alegre, RS 90040-060, Brazil.|EMBRAPA Recursos Genéticos e Biotecnologia , Parque Estação Biológica, W5 Norte, 70770-917 Brası́lia, Brazil.
authors: Marla J
authors: MJ
authors: Hassemer
authors: Universidade Federal do Rio Grande do Sul , PPG-Fitotecnia, Avenida Bento Gonçalves, 7712 Porto Alegre, RS 90040-060, Brazil.
authors: Josué
authors: J
authors: Sant'Ana
authors: EMBRAPA Recursos Genéticos e Biotecnologia , Parque Estação Biológica, W5 Norte, 70770-917 Brası́lia, Brazil.
authors: Miguel
authors: M
authors: Borges
authors: Biological Chemistry and Crop Protection Department, Rothamsted Research , Harpenden, Herts AL5 2JQ, United Kingdom.
authors: David
authors: D
authors: Withall
authors: Biological Chemistry and Crop Protection Department, Rothamsted Research , Harpenden, Herts AL5 2JQ, United Kingdom.
authors: John A
authors: JA
authors: Pickett
authors: EMBRAPA Recursos Genéticos e Biotecnologia , Parque Estação Biológica, W5 Norte, 70770-917 Brası́lia, Brazil.
authors: Márcio W M
authors: MW
authors: de Oliveira
authors: EMBRAPA Recursos Genéticos e Biotecnologia , Parque Estação Biológica, W5 Norte, 70770-917 Brası́lia, Brazil.
authors: Raul A
authors: RA
authors: Laumann
authors: Biological Chemistry and Crop Protection Department, Rothamsted Research , Harpenden, Herts AL5 2JQ, United Kingdom.
authors: Michael A
authors: MA
authors: Birkett
authors: EMBRAPA Recursos Genéticos e Biotecnologia , Parque Estação Biológica, W5 Norte, 70770-917 Brası́lia, Brazil.
authors: Maria C
authors: MC
authors: Blassioli-Moraes
mesh_terms: D000080462:Acyclic Monoterpenes; D000475:Alkenes / Q000737:chemistry; D000818:Animals; D001522:Behavior, Animal; D001938:Brazil; D001643:Bridged Bicyclo Compounds / Q000737:chemistry; D002633:Chemotaxis; D002849:Chromatography, Gas; D053138:Cyclohexenes / Q000737:chemistry; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007659:Ketones / Q000737:chemistry; D000077222:Limonene; D008297:Male; D039821:Monoterpenes / Q000737:chemistry; D064367:Olfactometry; D010675:Pheromones / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry; D013712:Tenebrio / Q000187:drug effects*; D013729:Terpenes / Q000737:chemistry; D055549:Volatile Organic Compounds / Q000737:chemistry
chemical_list: D000080462:Acyclic Monoterpenes; D000475:Alkenes; D001643:Bridged Bicyclo Compounds; D053138:Cyclohexenes; D007659:Ketones; D039821:Monoterpenes; D010675:Pheromones; D012717:Sesquiterpenes; D013729:Terpenes; D055549:Volatile Organic Compounds; C533056:daucene; C010411:insect attractants; C443996:beta-ocimene; C062672:alpha-farnesene; D000077222:Limonene; C018584:linalool; C026636:2-nonanone
keywords: Alphitobius diaperinus; aggregation pheromone; lesser mealworm