title: A viscosity sensitive fluorescent dye for real-time monitoring of mitochondria transport in neurons.
abstract: We present here a viscosity sensitive fluorescent dye, namely thiophene dihemicyanine (TDHC), that enables the specific staining of mitochondria. In comparison to the common mitochondria tracker (Mitotracker Deep Red, MTDR), this dye demonstrated its unique ability for robust staining of mitochondria with high photostability and ultrahigh signal-to-noise ratio (SNR). Moreover, TDHC also showed high sensitivity towards mitochondria membrane potential (ΔΨm) and intramitochondria viscosity change. Consequently, this dye was utilized in real-time monitoring of mitochondria transport in primary cortical neurons. Finally, the Two-Photon Microscopy (TPM) imaging ability of TDHC was also demonstrated.
authors: Department of Chemistry and Nano Science, EwhaWomans University, Seoul, 120-750, Republic of Korea.
authors: Yeonju
authors: Y
authors: Baek
authors: Department of Energy Systems Research, Ajou University, Suwon, 443-749, Republic of Korea.
authors: Sang Jun
authors: SJ
authors: Park
authors: Research Centre for Chemical Biology, Department of Chemistry, Yanbian University, Yanji, 133002, PR China; Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Yanbian University, Yanji, 133002, PR China. Electronic address: zhouxin@ybu.edu.cn.
authors: Xin
authors: X
authors: Zhou
authors: Department of Chemistry and Nano Science, EwhaWomans University, Seoul, 120-750, Republic of Korea.
authors: Gyungmi
authors: G
authors: Kim
authors: Department of Energy Systems Research, Ajou University, Suwon, 443-749, Republic of Korea. Electronic address: kimhm@ajou.ac.kr.
authors: Hwan Myung
authors: HM
authors: Kim
authors: Department of Chemistry and Nano Science, EwhaWomans University, Seoul, 120-750, Republic of Korea. Electronic address: jyoon@ewha.ac.kr.
authors: Juyoung
authors: J
authors: Yoon
mesh_terms: D001693:Biological Transport, Active / Q000502:physiology; D002478:Cells, Cultured; D003199:Computer Systems; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000502:physiology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D051336:Mitochondrial Membranes / Q000737:chemistry* / Q000502:physiology* / Q000648:ultrastructure; D009474:Neurons; D013876:Thiophenes / Q000737:chemistry*; D014783:Viscosity
chemical_list: D005456:Fluorescent Dyes; D013876:Thiophenes
keywords: Fluorescent probe; Mitochondria selective probe; Mitochondria transport in neurons; Two photon probe; Viscosity probe