title: A nanoparticle-based nicotine vaccine and the influence of particle size on its immunogenicity and efficacy.
abstract: Traditional hapten-protein conjugate nicotine vaccines have shown less than desired immunological efficacy due to their poor recognition and internalization by immune cells. We developed a novel lipid-polymeric hybrid nanoparticle-based nicotine vaccine to enhance the immunogenicity of the conjugate vaccine, and studied the influence of particle size on its immunogenicity and pharmacokinetic efficacy. The results demonstrated that the nanovaccines, regardless of size, could induce a significantly stronger immune response against nicotine compared to the conjugate vaccine. Particularly, a significantly higher anti-nicotine antibody titer was achieved by the 100 compared to the 500nm nanovaccine. In addition, both the 100 and 500nm nanovaccines reduced the distribution of nicotine into the brain significantly. The 100nm nanovaccine exhibited better pharmacokinetic efficacy than the 500nm nanovaccine in the presence of alum adjuvant. These results suggest that a lipid-polymeric nanoparticle-based nicotine vaccine is a promising candidate to treat nicotine dependence.
authors: Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, USA.
authors: Zongmin
authors: Z
authors: Zhao
authors: Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, USA.
authors: Yun
authors: Y
authors: Hu
authors: Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, USA.
authors: Reece
authors: R
authors: Hoerle
authors: Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, USA.
authors: Meaghan
authors: M
authors: Devine
authors: Minneapolis Medical Research Foundation, Minneapolis, MN, USA.
authors: Michael
authors: M
authors: Raleigh
authors: Minneapolis Medical Research Foundation, Minneapolis, MN, USA.
authors: Paul
authors: P
authors: Pentel
authors: Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, USA. Electronic address: chzhang2@vt.edu.
authors: Chenming
authors: C
authors: Zhang
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D005260:Female; D008807:Mice, Inbred BALB C; D053758:Nanoparticles; D009538:Nicotine / Q000494:pharmacology*; D010316:Particle Size; D014029:Tobacco Use Disorder / Q000628:therapy; D014612:Vaccines
chemical_list: D000276:Adjuvants, Immunologic; D014612:Vaccines; D009538:Nicotine
keywords: Anti-nicotine antibody; Lipid-polymeric hybrid nanoparticle; Nanovaccine size; Nicotine addiction; Nicotine vaccine