title: Novel Poly(3-hydroxybutyrate-g-vinyl alcohol) Polyurethane Scaffold for Tissue Engineering.
abstract: The design of new synthetic grafted poly(3-hydroxybutyrate) as composite 3D-scaffolds is a convenient alternative for tissue engineering applications. The chemically modified poly(3-hydroxybutyrate) is receiving increasing attention for use as biomimetic copolymers for cell growth. As of yet, these copolymers cannot be used efficiently because of the lack of good mechanical properties. Here, we address this challenge, preparing a composite-scaffold of grafted poly(3-hydroxybutyrate) polyurethane for the first time. However, it is unclear if the composite structure and morphology can also offer a biological application. We obtained the polyurethane by mixing a polyester hydroxylated resin with polyisocyanate and the modified polyhydroxyalkanoates. The results show that the poly(3-hydroxybutyrate) grafted with poly(vinyl alcohol) can be successfully used as a chain extender to form a chemically-crosslinked thermosetting polymer. Furthermore, we show a proposal for the mechanism of the polyurethane synthesis, the analysis of its morphology and the ability of the scaffolds for growing mammalian cells. We demonstrated that astrocytes isolated from mouse cerebellum, and HEK293 can be cultured in the prepared material, and express efficiently fluorescent proteins by adenoviral transduction. We also tested the metabolism of Ca(2+) to obtain evidence of the biological activity.
authors: Laboratorio de Neurobiología Molecular y Celular, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Querétaro 76230, México.
authors: Adriana Pétriz
authors: AP
authors: Reyes
authors: Laboratorio de Neurobiología Molecular y Celular, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Querétaro 76230, México.
authors: Ataúlfo
authors: A
authors: Martínez Torres
authors: Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, México D.F, 04510, México.
authors: Ma Del Pilar
authors: Mdel P
authors: Carreón Castro
authors: Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Querétaro 76230, México.
authors: José Rogelio
authors: JR
authors: Rodríguez Talavera
authors: Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Querétaro 76230, México.
authors: Susana Vargas
authors: SV
authors: Muñoz
authors: Facultad de Ciencias, Universidad Nacional Autónoma de México, México D.F, 04510, México.
authors: Víctor Manuel Velázquez
authors: VM
authors: Aguilar
authors: Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, México D.F, 04510, México.
authors: Maykel González
authors: MG
authors: Torres
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D008297:Male; D008422:Materials Testing; D051379:Mice; D011091:Polyesters / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; D011142:Polyvinyl Alcohol