title: Stress hormonal changes in the brain and plasma after acute noise exposure in mice.
abstract: OBJECTIVE
  To investigate the effects of acute noise stress on two amine stress hormones, norepinephrine (NE) and 5-hydroxyindoleacetic acid (5-HIAA) in the brain and plasma of mice after noise exposure.
  
  
  METHODS
  Mice were grouped into the control and noise groups. Mice in the noise group were exposed to white noise of 110dB sound pressure level for 60min. Auditory brainstem response thresholds, distortion product otoacoustic emissions, the organ of Corti grading scores, western blots of NE/5-HIAA in the whole brain and hippocampus, and the plasma levels of NE/5-HIAA were compared between the two groups.
  
  
  RESULTS
  Significant hearing loss and cochlear damage were demonstrated in the noise group. NE and 5-HIAA in the hippocampus were elevated in the noise group (p=0.019/0.022 for NE/5-HIAA vs. the control). Plasma levels of NE and 5-HIAA were not statistically different between the groups (p=0.052/0.671 for NE/5-HIAA).
  
  
  CONCLUSIONS
  Hearing loss with outer hair cell dysfunction and morphological changes of the organ of Corti after noise exposure in C57BL/6 mice proved the reliability of our animal model as an acute noise stress model. NE and 5-HIAA are suggested to be the potential biomarkers for acute noise stress in the hippocampus.
authors: Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea.
authors: Sang Gyun
authors: SG
authors: Jin
authors: Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea.
authors: Min Jung
authors: MJ
authors: Kim
authors: Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea.
authors: So Young
authors: SY
authors: Park
authors: Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea. Electronic address: snparkmd@catholic.ac.kr.
authors: Shi Nae
authors: SN
authors: Park
mesh_terms: D000818:Animals; D001309:Auditory Threshold; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D003051:Cochlea / Q000473:pathology; D004195:Disease Models, Animal; D016057:Evoked Potentials, Auditory, Brain Stem; D018072:Hair Cells, Auditory, Outer / Q000473:pathology*; D006317:Hearing Loss, Noise-Induced / Q000378:metabolism* / Q000473:pathology; D006624:Hippocampus / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000097:blood / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009622:Noise; D009638:Norepinephrine / Q000097:blood / Q000378:metabolism*; D009925:Organ of Corti / Q000473:pathology; D017084:Otoacoustic Emissions, Spontaneous; D015203:Reproducibility of Results
chemical_list: D006897:Hydroxyindoleacetic Acid; D009638:Norepinephrine
keywords: 5-HIAA; Cochlea; Noise-induced hearing loss; Norepinephrine; Organ of Corti; Serotonin; Stress hormone