title: Protein Corona Influences Cell-Biomaterial Interactions in Nanostructured Tissue Engineering Scaffolds.
abstract: Biomaterials are extensively used to restore damaged tissues, in the forms of implants (e.g. tissue engineered scaffolds) or biomedical devices (e.g. pacemakers). Once in contact with the physiological environment, nanostructured biomaterials undergo modifications as a result of endogenous proteins binding to their surface. The formation of this macromolecular coating complex, known as 'protein corona', onto the surface of nanoparticles and its effect on cell-particle interactions are currently under intense investigation. In striking contrast, protein corona constructs within nanostructured porous tissue engineering scaffolds remain poorly characterized. As organismal systems are highly dynamic, it is conceivable that the formation of distinct protein corona on implanted scaffolds might itself modulate cell-extracellular matrix interactions. Here, we report that corona complexes formed onto the fibrils of engineered collagen scaffolds display specific, distinct, and reproducible compositions that are a signature of the tissue microenvironment as well as being indicative of the subject's health condition. Protein corona formed on collagen matrices modulated cellular secretome in a context-specific mannerex-vivo, demonstrating their role in regulating scaffold-cellular interactions. Together, these findings underscore the importance of custom-designing personalized nanostructured biomaterials, according to the biological milieu and disease state. We propose the use of protein corona asin situbiosensor oftemporalandlocalbiomarkers.
authors: Stanford Cardiovascular Institute, Stanford, CA, 94305 USA.|Department of Pediatrics, Stanford University, 300 Pasteur Dr., Stanford, CA 94305.
authors: Vahid
authors: V
authors: Serpooshan
authors: Stanford Cardiovascular Institute, Stanford, CA, 94305 USA.|Division of Cardiovascular Medicine, Stanford University, 300 Pasteur Dr., Stanford, CA 94305.
authors: Morteza
authors: M
authors: Mahmoudi
authors: Department of Pediatrics, Stanford University, 300 Pasteur Dr., Stanford, CA 94305.
authors: Mingming
authors: M
authors: Zhao
authors: Sanford-Burnham Medical Research Institute, 10901 N. Torrey Pines Road, La Jolla, CA, 92037.
authors: Ke
authors: K
authors: Wei
authors: Biomaterials and Advanced Drug Delivery Laboratory, Stanford University, 300 Pasteur Dr., Stanford, CA 94305.
authors: Senthilkumar
authors: S
authors: Sivanesan
authors: Proteomics Facility, Sanford-Burnham Medical Research Institute, La Jolla, CA 92037.
authors: Khatereh
authors: K
authors: Motamedchaboki
authors: Biomaterials and Advanced Drug Delivery Laboratory, Stanford University, 300 Pasteur Dr., Stanford, CA 94305.
authors: Andrey V
authors: AV
authors: Malkovskiy
authors: Department of Cardiothoracic Surgery, Stanford University Medical Center, Stanford, CA 94305.
authors: Andrew B
authors: AB
authors: Gladstone
authors: Department of Cardiothoracic Surgery, Stanford University Medical Center, Stanford, CA 94305.
authors: Jeffrey E
authors: JE
authors: Cohen
authors: Stanford Cardiovascular Institute, Stanford, CA, 94305 USA.|Division of Cardiovascular Medicine, Stanford University, 300 Pasteur Dr., Stanford, CA 94305.
authors: Phillip C
authors: PC
authors: Yang
authors: Stanford Cardiovascular Institute, Stanford, CA, 94305 USA.|Biomaterials and Advanced Drug Delivery Laboratory, Stanford University, 300 Pasteur Dr., Stanford, CA 94305.
authors: Jayakumar
authors: J
authors: Rajadas
authors: Stanford Cardiovascular Institute, Stanford, CA, 94305 USA.|Department of Pediatrics, Stanford University, 300 Pasteur Dr., Stanford, CA 94305.
authors: Daniel
authors: D
authors: Bernstein
authors: Department of Cardiothoracic Surgery, Stanford University Medical Center, Stanford, CA 94305.
authors: Y Joseph
authors: YJ
authors: Woo
authors: Stanford Cardiovascular Institute, Stanford, CA, 94305 USA.|Department of Pediatrics, Stanford University, 300 Pasteur Dr., Stanford, CA 94305.
authors: Pilar
authors: P
authors: Ruiz-Lozano
keywords: collagen; in situ biosensor; personalized nanostructured biomaterials; protein corona; tissue engineering scaffold