title: Severe chronic osteomyelitis caused by Morganella morganii with high population diversity.
abstract: A case of chronic osteomyelitis probably caused by Morganella morganii, occurring over a period of 30 years, is reported. The organism was identified through a combination of sample culture, direct sequencing, and 16S RNA gene amplicon sequencing. Further whole-genome sequencing and population structure analysis of the isolates from the patient showed the bacterial population to be highly diverse. This case provides a valuable example of a long-term infection caused by an opportunistic pathogen, M. morganii, with high diversity, which might evolve during replication within the host.
authors: Department of Orthopedics, First Affiliated Hospital of PLA General Hospital, No. 51 Fucheng Road, Haidian District, Beijing 100048, China.
authors: Jialiang
authors: J
authors: Zhu
authors: Department of Medical Affairs, General Hospital of Beijing Military Region of PLA, Beijing, China.
authors: Haifeng
authors: H
authors: Li
authors: Department of Surgery Room, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, China.
authors: Li
authors: L
authors: Feng
authors: Construction Engineering Research Institute, Xi'an, Shaanxi Province, China.
authors: Min
authors: M
authors: Yang
authors: Department of Orthopedics, First Affiliated Hospital of PLA General Hospital, No. 51 Fucheng Road, Haidian District, Beijing 100048, China.
authors: Ronggong
authors: R
authors: Yang
authors: Department of Orthopedics, First Affiliated Hospital of PLA General Hospital, No. 51 Fucheng Road, Haidian District, Beijing 100048, China.
authors: Lin
authors: L
authors: Yang
authors: Department of Orthopedics, First Affiliated Hospital of PLA General Hospital, No. 51 Fucheng Road, Haidian District, Beijing 100048, China.
authors: Li
authors: L
authors: Li
authors: Biodynamic Optical Imaging Center, School of Life Science, Peking University, No. 5 Yiheyuan Road, Haidian District, Beijing 100871, China.
authors: Ruoyan
authors: R
authors: Li
authors: Biodynamic Optical Imaging Center, School of Life Science, Peking University, No. 5 Yiheyuan Road, Haidian District, Beijing 100871, China.
authors: Minshan
authors: M
authors: Liu
authors: Department of Orthopedics, First Affiliated Hospital of PLA General Hospital, No. 51 Fucheng Road, Haidian District, Beijing 100048, China.
authors: Shuxun
authors: S
authors: Hou
authors: Institute of Disease Control and Prevention, AMMS, No. 20, Dongdajie, Fengtai District, Beijing 100071, China. Electronic address: yuehuakebj@163.com.
authors: Yuehua
authors: Y
authors: Ke
authors: Department of Orthopedics, First Affiliated Hospital of PLA General Hospital, No. 51 Fucheng Road, Haidian District, Beijing 100048, China. Electronic address: tiantaindede@163.com.
authors: Wenfeng
authors: W
authors: Li
authors: Biodynamic Optical Imaging Center, School of Life Science, Peking University, No. 5 Yiheyuan Road, Haidian District, Beijing 100871, China. Electronic address: fbai@pku.edu.cn.
authors: Fan
authors: F
authors: Bai
mesh_terms: D000328:Adult; D044822:Biodiversity; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D008297:Male; D020613:Morganella morganii / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010019:Osteomyelitis / Q000382:microbiology*; D010802:Phylogeny
keywords: Chronic osteomyelitis; Genetic diversity; Morganella; Whole-genome sequencing