title: Development and Validation of Chemometric-Assisted Spectrophotometric Methods for Simultaneous Determination of Phenylephrine Hydrochloride and Ketorolac Tromethamine in Binary Combinations.
abstract: The present work describes new spectrophotometric methods for the simultaneous determination of phenylephrine hydrochloride and ketorolac tromethamine in their synthetic mixtures. The applied chemometric techniques are multivariate methods including classical least squares, principal component regression, and partial least squares. In these techniques, the concentration data matrix was prepared by using the synthetic mixtures containing these drugs dissolved in distilled water. The absorbance data matrix corresponding to the concentration data was obtained by measuring the absorbances at 16 wavelengths in the range 244-274 nm at 2 nm intervals in the zero-order spectra. The spectrophotometric procedures do not require any separation steps. The accuracy, precision, and linearity ranges of the methods have been determined, and analyzing synthetic mixtures containing the studied drugs has validated them. The developed methods were successfully applied to the synthetic mixtures and the results were compared to those obtained by a reported HPLC method.
authors: Tanta University, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, Tanta, Egypt 31111.
authors: Hamed M
authors: HM
authors: Elfatatry
authors: Tanta University, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, Tanta, Egypt 31111.
authors: Mokhtar M
authors: MM
authors: Mabrouk
authors: Tanta University, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, Tanta, Egypt 31111.
authors: Sherin F
authors: SF
authors: Hammad
authors: Tanta University, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, Tanta, Egypt 31111.
authors: Fotouh R
authors: FR
authors: Mansour
authors: Tanta University, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, Tanta, Egypt 31111.
authors: Amira H
authors: AH
authors: Kamal
authors: Tanta University, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, Tanta, Egypt 31111.
authors: Shoeb
authors: S
authors: Alahmad
mesh_terms: D002851:Chromatography, High Pressure Liquid; D020911:Ketorolac Tromethamine / Q000032:analysis*; D016018:Least-Squares Analysis; D010656:Phenylephrine / Q000032:analysis*; D025341:Principal Component Analysis; D012984:Software; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010656:Phenylephrine; D020911:Ketorolac Tromethamine