title: Pore structure and dielectric behaviour of the 3D collagen-DAC scaffolds designed for nerve tissue repair.
abstract: The design and selection of a suitable scaffold with well-defined pores size distribution and dielectric properties are critical features for neural tissue engineering. In this study we use mercury porosimetry and the dielectric spectroscopy in the alpha-dispersion region of the electric field to determine the microarchitecture and activation energy of collagen (Col) modified by 2,3 dialdehyde cellulose (DAC). The scaffold was synthesized in three steps: (i) preparation of DAC by oxidation of cellulose, (ii) construction of a 3D Col sponge-shape or film, (iii) cross-linkage of the Col samples using DAC. The activation energy needed to break the bonds formed by water in the Col-DAC composite is approximately 2 times lower than that in the unmodified Col. In addition, the magnitude of conductivity for modified Col at 70°C is approximately 40% lower than that recorded for the unmodified Col. The largest fraction, of which at least 70% of the total pore volume comprises the sponge, is occupied by pores ranging from 20 to 100μm in size. The knowledge on the dielectric behaviour and microstructure of the Col-DAC scaffold may prove relevant to neural tissue engineering focused on the regeneration of the nervous system.
authors: Department of Material and Commodity Sciences and Textile Metrology, Lodz University of Technology, Poland. Electronic address: krystyna.pietrucha@p.lodz.pl.
authors: Krystyna
authors: K
authors: Pietrucha
authors: Department of Bionics and Bioimpedance, University of Medical Sciences, Poznań, Poland.
authors: Ewa
authors: E
authors: Marzec
authors: Textile Research Institute, Lodz, Poland.
authors: Marcin
authors: M
authors: Kudzin
mesh_terms: D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry* / Q000302:isolation & purification; D058266:Dielectric Spectroscopy; D004553:Electric Conductivity; D009417:Nerve Tissue / Q000254:growth & development; D010084:Oxidation-Reduction; D016062:Porosity; D013499:Surface Properties; D013552:Swine; D013710:Tendons / Q000737:chemistry; D013816:Thermodynamics; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D024042:Collagen Type I; D002482:Cellulose; C058684:2,3-dialdehydocellulose
keywords: Collagen-DAC; Dielectric properties; Pore structure