title: Radical-scavenging activity of penicillin G, ampicillin, oxacillin, and dicloxacillin.
abstract: The aim of this study was to characterize the antioxidant activity of penicillin G (PG), ampicillin (AMP), oxacillin (OX) and dicloxacillin (DOX) through their reactivity towards reactive oxygen species (superoxide anion radical, O2•̅; hydroxyl radical, HO•; peroxyl radical, ROO•; hydrogen peroxide, H2O2; DPPH•) using various in vitro antioxidant assays with chemiluminescence (CL) and spectrophotometry as measurement techniques. In hydroxyl radical assays , PG, OX and AMP were found to inhibit the CL signal arising from the Fenton-like reaction in a dose-dependent manner with IC50= 0.480 ± 0.020 mM, IC50= 0.569 ± 0.021 mM, and IC50= 0.630 ± 0.019 mM, respectively. The highest reactivity of PG among the tested penicillins towards the HO radical was confirmed in the deoxyribose degradation assay. In the ABAP-derived ROO radical assay, the radical-scavenging ability of the test penicillins was in the following order: AMP > PG > DOX > OX. The number of reduced DPPH radicals by the drugs tested was <1 being the biggest for PG. The weak antioxidant capacity of the test penicillins was confirmed in the trolox antioxidant capacity assay (0.075 ± 0.004; 0.093 ± 0.006; 0.123 ± 0.005; 0.126 ± 0.004) for OX, AMP, DOX, PG, respectively. Use of luminol as a CL probe for estimation of penicillin reactivity towards H2O2showed that only AMP was able to quench light emission; the remaining antibiotics demonstrated a strong enhancing effect. All the examined compounds showed a weak antioxidant potential when estimated using the ferric-ferrozine assay. This study is the first to report the evaluation of test penicillins as antioxidants under the same reaction conditions.
authors: Institute of Physics, Faculty of Mechanical Engineering and Mechatronics, West Pomeranian University of Technology in Szczecin, Al. Piastów 48/49, 70-311, Szczecin, Poland.
authors: Paweł
authors: P
authors: Berczyński
authors: Department of History of Medicine and Medical Ethics, Pomeranian Medical University, Rybacka 1, 70-204, Szczecin, Poland.
authors: Aleksandra
authors: A
authors: Kładna
authors: Institute of Physics, Faculty of Mechanical Engineering and Mechatronics, West Pomeranian University of Technology in Szczecin, Al. Piastów 48/49, 70-311, Szczecin, Poland.
authors: Irena
authors: I
authors: Kruk
authors: Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Giza, 12622, Egypt.
authors: Hassan Y
authors: HY
authors: Aboul-Enein
mesh_terms: D000667:Ampicillin / Q000737:chemistry*; D004009:Dicloxacillin / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D015394:Molecular Structure; D010068:Oxacillin / Q000737:chemistry*; D010400:Penicillin G / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D000667:Ampicillin; D004009:Dicloxacillin; D010400:Penicillin G; D010068:Oxacillin
keywords: antioxidant action; chemiluminescence; penicillins; reactive oxygen species; spectrophotometry