title: Enzymatic biosensor of horseradish peroxidase immobilized on Au-Pt nanotube/Au-graphene for the simultaneous determination of antioxidants.
abstract: A new electrochemical method has been proposed for the simultaneous determination of butylated hydroxyanisole (BHA) and propyl gallate (PG) in food matrices based on enzymatic biosensors. Spiny Au-Pt nanotubes (SAP NTs) was first synthesized and demonstrated to exhibit intrinsic peroxidase and catalase-like activity. The structure of SAP NTs provides large surface area and favorable medium for electron transfer, on which HRP were immobilized and acted as enzymatic biosensor for the simultaneous detection of BHA and PG. The results revealed that BHA and PG both have well-defined oxidation waves with peak potentials of 624 and 655 mV, respectively. Under the optimal conditions, the method behaved satisfactory analytical performance towards BHA and PG with a wide linear range of 0.3-50 mg L(-1) and 0.1-100 mg L(-1), as well as a detection limit of 0.046 mg L(-1) and 0.024 mg L(-1) (3σ/slope), respectively. Besides, the proposed method exhibits good sensitivity, stability and reproducibility, providing an alternative to fabricate electrode and construct sensitive biosensors.
authors: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Long
authors: L
authors: Wu
authors: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Wenmin
authors: W
authors: Yin
authors: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Kun
authors: K
authors: Tang
authors: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Dian
authors: D
authors: Li
authors: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Kang
authors: K
authors: Shao
authors: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Yunpeng
authors: Y
authors: Zuo
authors: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Jing
authors: J
authors: Ma
authors: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Jiawei
authors: J
authors: Liu
authors: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: hyhan@mail.hzau.edu.cn.
authors: Heyou
authors: H
authors: Han
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006046:Gold / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D043942:Nanotubes / Q000737:chemistry; D010984:Platinum / Q000737:chemistry / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004800:Enzymes, Immobilized; D010984:Platinum; D006046:Gold; D006108:Graphite; D006735:Horseradish Peroxidase
keywords: Antioxidants; Enzymatic biosensors; Horseradish peroxidase; Linear-sweep voltammetry; Spiny Pt-Au nanotube