title: Effects of dietary supplementation with EPA and/or α-lipoic acid on adipose tissue transcriptomic profile of healthy overweight/obese women following a hypocaloric diet.
abstract: In obesity, the increment of adiposity levels disrupts the whole body homeostasis, promoting an over production of oxidants and inflammatory mediators. The current study aimed to characterize the transcriptomic changes promoted by supplementation with eicosapentaenoic acid (EPA, 1.3 g/day), α-lipoic acid (0.3 g/day), or both (EPA + α-lipoic acid, 1.3 g/day + 0.3 g/day) in subcutaneous abdominal adipose tissue from overweight/obese healthy women, who followed a hypocaloric diet (30% of total energy expenditure) during ten weeks, by using a microarray approach. At the end of the intervention, a total of 33,297 genes were analyzed using Affymetrix GeneChip arrays. EPA promoted changes in extracellular matrix remodeling gene expression, besides a rise of genes associated with either chemotaxis or wound repair. α-Lipoic acid decreased expression of genes related with cell adhesion and inflammation. Furthermore, α-lipoic acid, especially in combination with EPA, upregulated the expression of genes associated with lipid catabolism while downregulated genes involved in lipids storage. Together, all these data suggest that some of the metabolic effects of EPA and α-lipoic acid could be related to their regulatory actions on adipose tissue metabolism. © 2016 BioFactors, 43(1):117-131, 2017.
authors: Department of Nutrition, Food Science and Physiology, University of Navarra, Pamplona, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.
authors: Ana E
authors: AE
authors: Huerta
authors: Department of Nutrition, Food Science and Physiology, University of Navarra, Pamplona, Spain.
authors: Pedro L
authors: PL
authors: Prieto-Hontoria
authors: Department of Nutrition, Food Science and Physiology, University of Navarra, Pamplona, Spain.
authors: Marta
authors: M
authors: Fernández-Galilea
authors: Department of Nutrition, Food Science and Physiology, University of Navarra, Pamplona, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.
authors: Xavier
authors: X
authors: Escoté
authors: Department of Nutrition, Food Science and Physiology, University of Navarra, Pamplona, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.|Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition (CIBERobn), Institute of Health Carlos III (ISCIII), Madrid, Spain.|Navarra Institute for Health Research (IdiSNA), Pamplona, Spain.
authors: J Alfredo
authors: JA
authors: Martínez
authors: Department of Nutrition, Food Science and Physiology, University of Navarra, Pamplona, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.|Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition (CIBERobn), Institute of Health Carlos III (ISCIII), Madrid, Spain.|Navarra Institute for Health Research (IdiSNA), Pamplona, Spain.
authors: María J
authors: MJ
authors: Moreno-Aliaga
mesh_terms: D050153:Abdominal Fat / Q000378:metabolism; D000284:Administration, Oral; D000328:Adult; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D031204:Caloric Restriction; D019587:Dietary Supplements; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D053858:Metabolic Networks and Pathways; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy* / Q000378:metabolism; D008063:Thioctic Acid / Q000008:administration & dosage*; D059467:Transcriptome
chemical_list: D000893:Anti-Inflammatory Agents; D008063:Thioctic Acid; D015118:Eicosapentaenoic Acid
keywords: adipose tissue; gene expression; microarray; n3-PUFAs; obesity