title: Mineralogenic characteristics of osteogenic lineage-committed human dental pulp stem cells following their exposure to a discoloration-free calcium aluminosilicate cement.
abstract: An experimental discoloration-free calcium aluminosilicate cement has been developed with the intention of maximizing the beneficial attributes of tricalcium silicate cements and calcium aluminate cements. The present study examined the effects of this experimental cement (Quick-Set2) on the mineralogenic characteristics of osteogenic lineage-committed human dental pulp stem cells (hDPSCs), by comparing the cellular responses with a commercially available tricalcium silicate cement (white mineral trioxide aggregate (ProRoot(®) MTA); WMTA).
  
  
  
  The osteogenic potential of hDPSCs exposed to the cements was examined using qRT-PCR for osteogenic gene expressions, Western blot for osteogenic-related protein expressions, alkaline phosphatase enzyme activity, Alizarin red S staining, Fourier transform infrared spectroscopy and transmission electron microscopy of extracellular calcium deposits.
  
  
  
  Results of the six assays indicated that osteogenic differentiation of hDPSCs was significantly enhanced after exposure to the tricalcium silicate cement or the experimental calcium aluminosilicate cement, with the former demonstrating better mineralogenic stimulation capacity.
  
  
  
  The better osteogenic stimulating effect of the tricalcium silicate cement on hDPSCs may be due to its relatively higher silicate content, or higher OH(-) and Ca(2+) release. Further investigations with the use of in vivo animal models are required to validate the potential augmenting osteogenic effects of the experimental discoloration-free calcium aluminosilicate cement.
authors: State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China; Department of Endodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA. Electronic address: niulina831013@126.com.
authors: Li-Na
authors: LN
authors: Niu
authors: Department of Prosthodontics, Stomatology Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
authors: Dan-Dan
authors: DD
authors: Pei
authors: Department of Endodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA.
authors: Matthew
authors: M
authors: Morris
authors: State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors: Kai
authors: K
authors: Jiao
authors: Department of Prosthodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors: Xue-Qing
authors: XQ
authors: Huang
authors: LECOM School of Dental Medicine, Bradenton, FL, USA.
authors: Carolyn M
authors: CM
authors: Primus
authors: Department of Endodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA.
authors: Lisiane F
authors: LF
authors: Susin
authors: Department of Endodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA.
authors: Brian E
authors: BE
authors: Bergeron
authors: Department of Oral Biology, The Dental College of Georgia, Augusta University, Augusta, GA, USA.
authors: David H
authors: DH
authors: Pashley
authors: Department of Endodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA. Electronic address: ftay@gru.edu.
authors: Franklin R
authors: FR
authors: Tay
mesh_terms: D000538:Aluminum Silicates / Q000494:pharmacology*; D000818:Animals; D017610:Calcium Compounds; D000077215:Clay; D003738:Dental Cements / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects*; D017640:Silicates; D013234:Stem Cells
chemical_list: D000538:Aluminum Silicates; D017610:Calcium Compounds; D003738:Dental Cements; D017640:Silicates; D000077215:Clay
keywords: Calcium aluminosilicate cement; Human dental pulp stem cells; Mineral trioxide aggregate; Osteogenic differentiation