title: The effect of Nigella sativa oil on prevention of myringosclerosis in a Guinea pig model.
abstract: OBJECTIVE
  In this study, our aim was to identify the possible effects of Nigella sativa L. (NS) [blackcumin] seed oil on the prevention of experimentally induced myringosclerosis (MS).
  
  
  METHODS
  Fourteen Guinea pigs were used and they were divided into three groups. Tympanic membranes (TM) of all animals were perforated and then group I was treated with saline soaked gel foams as a control group, group II was treated with 0.5 ml NS oil soaked gel foams at 0, 24 and 48 h and group III was treated with 5 ml NS oil orally at 0, 24, 48, 72 and 120 h. After 15 days, all animals were euthanized. Tympanic membranes were evaluated macroscopically and histopathologically.
  
  
  RESULTS
  Groups I showed extensive myringosclerosis in contrast to those of Groups II and III which had significantly less changes (p < 0.05). The fibrosis and inflammation in the lamina propria of the tympanic membranes of Groups I was found to be significantly more pronounced (p < 0.05). The tympanic membranes were found to be significantly thinner in Groups II and III when compared with Groups I (p < 0.05).
  
  
  CONCLUSIONS
  The results of this study suggested that topical or oral administration of NS oil supressed the inflammation and fibroblastic activity in the lamina propria of the myringotomized TMs of the Guinea pigs. For providing further evidence to use plant extracts as antioxidant and antiinflammatory therapy after myringotomy or ventilation tube insertion, further clinical studies with larger population will be essential.
authors: Istanbul Medeniyet University, Faculty of Medicine, Göztepe Training and Research Hospital, Department of Otorhinolaryngology, Istanbul, Turkey.
authors: Numan
authors: N
authors: Kokten
authors: Malkara State Hospital, ENT Clinic, Tekirdağ, Turkey.
authors: Oguz Kadir
authors: OK
authors: Egilmez
authors: Yeditepe University, Faculty of Medicine, Department of Pathology, Istanbul, Turkey.
authors: A Isin
authors: AI
authors: Dogan Ekici
authors: Istanbul Medeniyet University, Faculty of Medicine, Göztepe Training and Research Hospital, Department of Otorhinolaryngology, Istanbul, Turkey. Electronic address: mtkalcioglu@hotmail.com.
authors: M Tayyar
authors: MT
authors: Kalcioglu
authors: Istanbul Medeniyet University, Faculty of Medicine, Göztepe Training and Research Hospital, Department of Otorhinolaryngology, Istanbul, Turkey.
authors: Muhammet
authors: M
authors: Tekin
authors: Yeditepe University, Faculty of Pharmacy, Department of Pharmacognosy, Istanbul, Turkey.
authors: Erdem
authors: E
authors: Yesilada
mesh_terms: D000284:Administration, Oral; D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D005355:Fibrosis; D006168:Guinea Pigs; D007249:Inflammation / Q000473:pathology; D008297:Male; D008876:Middle Ear Ventilation; D009092:Mucous Membrane / Q000187:drug effects* / Q000473:pathology; D063371:Myringosclerosis / Q000473:pathology / Q000517:prevention & control*; D010938:Plant Oils / Q000494:pharmacology*; D014432:Tympanic Membrane / Q000187:drug effects* / Q000473:pathology; D029974:Carum
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010938:Plant Oils; C104525:caraway oil
keywords: Antiinflammatory; Guinea pig; Myringosclerosis; Nigella sativa oil