title: One-step competitive lateral flow biosensor running on an independent quantification system for smart phones based in-situ detection of trace Hg(II) in tap water.
abstract: In this study, a one-step lateral flow biosensor (LFB) has been developed, optimized and validated for quantitative detection of Hg(II) in water. In the measurement principle, just one T-rich ssDNA probe (TSP) for the specific binding process was successfully employed in the competitive LFB based methods. The concept of an independent quantification system was realized using a cresol red dot as an external standard, which effectively eliminates false negative results. Under optimized conditions, the limit of detection for Hg(II) was 4nM; high selectivity towards Hg(II) and extraordinary device-to-device repeatability of the LFB were achieved. Furthermore, Hg(II) from tap water samples was analyzed, and the results were confirmed by ICP-MS. The interference from other components in the real samples could be neglected during the analysis. The approach provides a simple, sensitive, and practical tool for the detection of trace Hg(II) in tap water, showing great promise for in-situ applications.
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors: Nan
authors: N
authors: Cheng
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors: Yuancong
authors: Y
authors: Xu
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China; Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors: Kunlun
authors: K
authors: Huang
authors: Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors: Yuting
authors: Y
authors: Chen
authors: Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors: Zhansen
authors: Z
authors: Yang
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors: Yunbo
authors: Y
authors: Luo
authors: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China; Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: xuwentao@cau.edu.cn.
authors: Wentao
authors: W
authors: Xu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis*; D000068997:Smartphone; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D014874:Water Pollutants, Chemical; D014867:Water; D008628:Mercury
keywords: Competitive lateral flow biosensor; Independent quantification system; Smart phones; Trace Hg(II); Water detection