title: Genomic and functional features of the biosurfactant producing Bacillus sp. AM13.
abstract: Genomic studies provide deeper insights into secondary metabolites produced by diverse bacterial communities, residing in various environmental niches. This study aims to understand the potential of a biosurfactant producing Bacillus sp. AM13, isolated from soil. An integrated approach of genomic and chemical analysis was employed to characterize the antibacterial lipopeptide produced by the strain AM13. Genome analysis revealed that strain AM13 harbors a nonribosomal peptide synthetase (NRPS) cluster; highly similar with known biosynthetic gene clusters from surfactin family: lichenysin (85 %) and surfactin (78 %). These findings were substantiated with supplementary experiments of oil displacement assay and surface tension measurements, confirming the biosurfactant production. Further investigation using LCMS approach exhibited similarity of the biomolecule with biosurfactants of the surfactin family. Our consolidated effort of functional genomics provided chemical as well as genetic leads for understanding the biochemical characteristics of the bioactive compound.
authors: Department of Biochemistry, Government Medical College, Nagpur, 440009, Maharashtra, India.|Microbial Culture Collection, National Centre for Cell Science, Pune, 411007, Maharashtra, India.
authors: Shraddha
authors: S
authors: Shaligram
authors: Microbial Culture Collection, National Centre for Cell Science, Pune, 411007, Maharashtra, India.
authors: Shreyas V
authors: SV
authors: Kumbhare
authors: Microbial Culture Collection, National Centre for Cell Science, Pune, 411007, Maharashtra, India.
authors: Dhiraj P
authors: DP
authors: Dhotre
authors: Department of Biochemistry, Government Medical College, Nagpur, 440009, Maharashtra, India. mmuddeshwar2016@gmail.com.
authors: Manohar G
authors: MG
authors: Muddeshwar
authors: Environmental Genomics Division, National Environmental Engineering Research Institute, CSIR Nehru Marg, Nagpur, 440020, India.
authors: Atya
authors: A
authors: Kapley
authors: Microbial Culture Collection, National Centre for Cell Science, Pune, 411007, Maharashtra, India.
authors: Neetha
authors: N
authors: Joseph
authors: Environmental Genomics Division, National Environmental Engineering Research Institute, CSIR Nehru Marg, Nagpur, 440020, India.
authors: Hemant P
authors: HP
authors: Purohit
authors: Microbial Culture Collection, National Centre for Cell Science, Pune, 411007, Maharashtra, India.
authors: Yogesh S
authors: YS
authors: Shouche
authors: Microbial Culture Collection, National Centre for Cell Science, Pune, 411007, Maharashtra, India. shrikantpawar@nccs.res.in.
authors: Shrikant P
authors: SP
authors: Pawar
mesh_terms: D001407:Bacillus / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D016680:Genome, Bacterial; D023281:Genomics; D010453:Peptide Synthases / Q000235:genetics* / Q000302:isolation & purification; D064210:Secondary Metabolism / Q000235:genetics; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D013501:Surface-Active Agents; D010453:Peptide Synthases
keywords: Bacillus genome; Bacterial natural products; Biosurfactant; Lipopeptides; NRPS