title: Comparison of Phytochemical Composition and Biological Activities of Rubus ulmifolius Extracts Originating from Four Regions of Tunisia.
abstract: In the current study, the phenolic composition, antioxidant and antimicrobial activities of extracts from Rubus ulmifolius Schott leaves harvested in four localities (Sejnen, Tabarka, Faija and Ain drahem) in Tunisia were investigated for the first time. Great differences were found for the chemical composition, total phenol contents and biological activities among the evaluated extracts. HPLC analysis of methanolic extracts showed that the dominant compounds were kaempferol 3-O-rutinoside and naringenine. In addition, significant correlations were observed between antioxidant activities and phenolic contents. In fact, leaves collected from Sejnen presented higher total phenol content (53.32 mg GAE/g DW) and antioxidant activities (IC50= 39.40 mg/l) than the others samples. All extracts showed significant antimicrobial activity against six used bacteria with the inhibition zones diameters and minimal inhibitory concentration values were in the range of 8 - 16 mm and 6.25 - 25 mg/ml, respectively. The highest antimicrobial activities were recorded in Sejnen extract against Gram-positive bacteria.
authors: Laboratory of Sylvo - Pastoral Resources of Tabarka, Institute of Sylvo-Pastoral, Jendouba University, 8110, Tabarka, Tunisia.|Laboratory of Medicinal and Aromatic Plants, LPAM, Biotechnology Center of Borj Cedria, (CBBC), BP 901, 2050, Hammam-Lif, Tunisia.
authors: Sonia
authors: S
authors: Tabarki
authors: Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology, Pasteur Institute of Tunisia (IPT), BP 74, 13 place Pasteur, Belvédère, 1002, Tunis, Tunisia.
authors: Chedia
authors: C
authors: Aouadhi
authors: National Institute for Research in Rural Engineering, Water and Forestry, Street Hedi Karay PB 10, 2080, Ariana, Tunisia.
authors: Kaouther
authors: K
authors: Mechergui
authors: Laboratory of Medicinal and Aromatic Plants, LPAM, Biotechnology Center of Borj Cedria, (CBBC), BP 901, 2050, Hammam-Lif, Tunisia.
authors: Khaoula Mkadmini
authors: KM
authors: Hammi
authors: Laboratory of Medicinal and Aromatic Plants, LPAM, Biotechnology Center of Borj Cedria, (CBBC), BP 901, 2050, Hammam-Lif, Tunisia.
authors: Riadh
authors: R
authors: Ksouri
authors: Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis EL Manar, 2092 EL Manar II, Tunis, Tunisia.
authors: Aly
authors: A
authors: Raies
authors: Laboratory of Sylvo - Pastoral Resources of Tabarka, Institute of Sylvo-Pastoral, Jendouba University, 8110, Tabarka, Tunisia.
authors: Lamjed
authors: L
authors: Toumi
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D065856:Rubus / Q000737:chemistry*; D014416:Tunisia
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts
keywords: 
  Rubus ulmifolius
  ; Antibacterial activity; Antioxidant activity; Chemical composition; Secondary metabolites