title: Evaluation of a Commercial Real-Time PCR Kit for the Detection of Mycobacterium avium subspecies paratuberculosis in Milk.
abstract: There are several commercial test kits for Mycobacterium avium subspecies paratuberculosis (MAP) detection, each with different advantages, disadvantages, and applications. In the present study, a real-time PCR kit targeting the unique transposon sequence ISMAP02 was evaluated. The analytical sensitivity was determined using the type strain ATCC 19698, and the specificity was validated by testing fifteen MAP isolates, thirteen non-MAP Mycobacterium isolates, and eight non-Mycobacterium isolates. Six spiking experiments were performed using raw milk and reconstituted infant milk artificially contaminated with dilutions containing 10(0)-10(5) MAP cells mL(-1). Sensitivity and specificity were at 100 %. The detection probabilities in raw milk and reconstituted infant milk for the samples (containing 1.4 × 10(1) and 1.7 × 10(1) MAP cell 50 mL(-1)) were 16.6 and 91.6 %, respectively. Thus, the tested kit yielded satisfying results to detect MAP in milk.
authors: Public Authority of Agriculture Affairs & Fish Resources (PAAF), P.O. Box 21422, 13075, Safat, Kuwait.|Institute for Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173, Hannover, Germany.
authors: Ahmad
authors: A
authors: Alajmi
authors: Institute for Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173, Hannover, Germany.
authors: Günter
authors: G
authors: Klein
authors: Institute for Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173, Hannover, Germany.
authors: Nils Th
authors: NT
authors: Grabowski
authors: Institute for Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173, Hannover, Germany.
authors: Svenja
authors: S
authors: Fohler
authors: Dairy Sciences, Institute of Veterinary Food Science, Justus-Liebig University Giessen, Ludwigstrasse 21, 35390, Giessen, Germany.
authors: Ömer
authors: Ö
authors: Akineden
authors: Institute for Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173, Hannover, Germany. amir.abdulmawjood@tiho-hannover.de.
authors: Amir
authors: A
authors: Abdulmawjood
mesh_terms: D000818:Animals; D002417:Cattle; D005506:Food Contamination / Q000032:analysis*; D041943:Infant Formula / Q000382:microbiology; D008892:Milk / Q000382:microbiology*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic