title: Improved Mechanical Properties and Sustained Release Behavior of Cationic Cellulose Nanocrystals Reinforeced Cationic Cellulose Injectable Hydrogels.
abstract: Polysaccharide-based injectable hydrogels have several advantages in the context of biomedical use. However, the main obstruction associated with the utilization of these hydrogels in clinical application is their poor mechanical properties. Herein, we describe in situ gelling of nanocomposite hydrogels based on quaternized cellulose (QC) and rigid rod-like cationic cellulose nanocrystals (CCNCs), which can overcome this challenge. In all cases, gelation immediately occurred with an increase of temperature, and the CCNCs were evenly distributed throughout the hydrogels. The nanocomposite hydrogels exhibited increasing orders-of-magnitude in the mechanical strength, high extension in degradation and the sustained release time, because of the strong interaction between CCNCs and QC chains mediated by the cross-linking agent (β-glycerophosphate, β-GP). The results of the in vitro toxicity and in vivo biocompatibility tests revealed that the hydrogels did not show obvious cytotoxicity and inflammatory reaction to cells and tissue. Moreover, DOX-encapsulated hydrogels were injected beside the tumors of mice bearing liver cancer xenografts to assess the potential utility as localized and sustained drug delivery depot systems for anticancer therapy. The results suggested that the QC/CCNC/β-GP nanocomposite hydrogels had great potential for application in subcutaneous and sustained delivery of anticancer drug to increase therapeutic efficacy and improve patient compliance.
authors: Department of Chemistry and Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, China.|CAS Key Lab of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao 266101, China.
authors: Jun
authors: J
authors: You
authors: Department of Chemistry and Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, China.
authors: Jinfeng
authors: J
authors: Cao
authors: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University , Wuhan 430071, China.
authors: Yanteng
authors: Y
authors: Zhao
authors: Department of Chemistry and Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, China.
authors: Lina
authors: L
authors: Zhang
authors: Department of Chemistry and Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, China.
authors: Jinping
authors: J
authors: Zhou
authors: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University , Wuhan 430071, China.
authors: Yun
authors: Y
authors: Chen
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology; D002412:Cations / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002482:Cellulose / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology; D016503:Drug Delivery Systems; D005260:Female; D005994:Glycerophosphates / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000008:administration & dosage* / Q000737:chemistry; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D023822:Tissue Engineering; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D000903:Antibiotics, Antineoplastic; D002412:Cations; D003692:Delayed-Action Preparations; D005994:Glycerophosphates; D020100:Hydrogels; D004317:Doxorubicin; D002482:Cellulose; C031463:beta-glycerophosphoric acid