title: An LC-MS/MS method for simultaneous determination of hosenkoside A and hosenkoside K from Semen Impatientis in rat plasma and its application to a pharmacokinetic study.
abstract: A rapid and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of two baccharane glycosides (hosenkoside A and hosenkoside K) of total saponins of Semen Impatientis in rat plasma using mogroside V as the internal standard (IS). The analytes were separated using a C18RP Agilent XDB column (1.8 μm, 50 × 2.1 mm i.d.) and detection of the compounds was done using a TSQ Quantum triple quadrupole mass spectrometer coupled with a negative electrospray ionization source under selection reaction monitoring mode. According to the US Food and Drug Administration guidelines, the established method was fully validated and the results were proved within acceptable limits. The lower limits of quantification of both analytes were 5 ng/mL. The validated method was successfully applied to a pharmacokinetic study of orally administered the total saponins of Semen Impatientis in rats.
authors: Department of Emergency, Linyi People's Hospital, Linyi, China.
authors: Xiaodong
authors: X
authors: Yu
authors: Department of Traumatologic Surgery, Linyi People's Hospital, Linyi, China.
authors: Binna
authors: B
authors: Bi
authors: Department of Neurosurgery, Chinese Medicine Hospital in Linyi City, Linyi, China.
authors: Ning
authors: N
authors: Dong
authors: Department of Emergency, Linyi People's Hospital, Linyi, China.
authors: Huanli
authors: H
authors: Zhao
authors: Department of Spine Surgery, Linyi People's Hospital, Linyi, China.
authors: Lixin
authors: L
authors: Ji
authors: Department of Spine Surgery, Linyi People's Hospital, Linyi, China.
authors: Minghua
authors: M
authors: Lu
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000032:analysis / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry; D014315:Triterpenes / Q000032:analysis / Q000493:pharmacokinetics
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C557483:Impatientis Semen; C041740:mogroside V
keywords: LC-MS/MS; Semen Impatientis; hosenkoside A; hosenkoside K; pharmacokinetic study