title: Chip-based molecularly imprinted monolithic capillary array columns coated GO/SiO2 for selective extraction and sensitive determination of rhodamine B in chili powder.
abstract: A novel solid-phase extraction chip embedded with array columns of molecularly imprinted polymer-coated silanized graphene oxide (GO/SiO2-MISPE) was established to detect trace rhodamine B (RB) in chili powder. GO/SiO2-MISPE monolithic columns for RB detection were prepared by optimizing the supporting substrate, template, and polymerizing monomer under mild water bath conditions. Adsorption capacity and specificity, which are critical properties for the application of the GO/SiO2-MISPE monolithic column, were investigated. GO/SiO2-MIP was examined by scanning electron microscopy (SEM) and Fourier transform-infrared spectroscopy. The recovery and the intraday and interday relative standard deviations for RB ranged from 83.7% to 88.4% and 2.5% to 4.0% and the enrichment factors were higher than 110-fold. The chip-based array columns effectively eliminated impurities in chili powder, indicating that the chip-based GO/SiO2-MISPE method was reliable for RB detection in food samples using high-performance liquid chromatography. Accordingly, this method has direct applications for monitoring potentially harmful dyes in processed food.
authors: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: zhaihaiyun@126.com.
authors: Haiyun
authors: H
authors: Zhai
authors: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors: Lu
authors: L
authors: Huang
authors: School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou 510006, China.
authors: Zuanguang
authors: Z
authors: Chen
authors: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors: Zihao
authors: Z
authors: Su
authors: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors: Kaisong
authors: K
authors: Yuan
authors: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors: Guohuan
authors: G
authors: Liang
authors: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors: Yufang
authors: Y
authors: Pan
mesh_terms: D000327:Adsorption; D002212:Capsicum / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D057230:Limit of Detection; D044085:Microfluidics; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D010087:Oxides / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000737:chemistry; D012235:Rhodamines / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction; D017550:Spectroscopy, Fourier Transform Infrared; D017365:Spices / Q000032:analysis*
chemical_list: D004396:Coloring Agents; D004129:Dimethylpolysiloxanes; D010087:Oxides; D011108:Polymers; D012235:Rhodamines; C013830:baysilon; D012822:Silicon Dioxide; D006108:Graphite; C029773:rhodamine B
keywords: Chili powder; Micro-fluidic chip; Molecularly imprinted solid-phase extraction; Monolithic array column; Rhodamine B