title: Diethylnitrosamine-induced expression of germline-specific genes and pluripotency factors, including vasa and oct4, in medaka somatic cells.
abstract: Various methods have been developed to reprogram mammalian somatic cells into pluripotent cells as well as to directly reprogram somatic cells into other cell lineages. We are interested in applying these methods to fish, and here, we examined whether mRNA expression of germline-specific genes (vasa, nanos2, -3) and pluripotency factors (oct4, sox2, c-myc, nanog) is inducible in somatic cells of Japanese medaka (Oryzias latipes). We found that the expression of vasa is induced in the gut and regenerating fin by exposure to a carcinogen, diethylnitrosamine (DEN). Induction of vasa in the gut started on the 5th day of treatment with >50 ppm DEN. In addition, nanos2, -3, oct4, sox2, klf4, c-myc, and nanog were also expressed simultaneously in some vasa-positive gut and regenerating fin samples. Vasa-positive cells were detected by immunohistochemistry (IHC) in the muscle surrounding the gut and in the wound epidermis, blastema, and fibroblast-like cells in regenerating fin. In vasa:GFP transgenic medaka, green fluorescent protein (GFP) fluorescence appeared in the wound epidermis and fibroblast-like cells in the regenerating fin following DEN exposure, in agreement with the IHC data. Our data show that mRNA expression of genes relevant to germ cell specification and pluripotency can be induced in fish somatic cells by exposure to DEN, suggesting the possibility of efficient and rapid cell reprogramming of fish somatic cells.
authors: Laboratory of Marine Life Science and Genetics, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors: Jialing
authors: J
authors: Shen
authors: Laboratory of Marine Life Science and Genetics, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors: Shinpei
authors: S
authors: Yokota
authors: Laboratory of Marine Life Science and Genetics, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors: Hayato
authors: H
authors: Yokoi
authors: Laboratory of Marine Life Science and Genetics, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan. Electronic address: toru.suzuki.a8@tohoku.ac.jp.
authors: Tohru
authors: T
authors: Suzuki
mesh_terms: D058500:Animal Fins / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D015415:Biomarkers / Q000378:metabolism; D002273:Carcinogens / Q000494:pharmacology*; D065150:Cellular Reprogramming; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism; D004052:Diethylnitrosamine / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000187:drug effects; D054792:Muscle, Striated / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000071317:Nanog Homeobox Protein / Q000235:genetics / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D009990:Oryzias; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D016335:Zinc Fingers
chemical_list: D015415:Biomarkers; D002273:Carcinogens; D029941:Fish Proteins; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D016271:Proto-Oncogene Proteins c-myc; D016601:RNA-Binding Proteins; D055748:SOXB1 Transcription Factors; D004052:Diethylnitrosamine; D053487:DEAD-box RNA Helicases
keywords: Cancer; Cell reprogramming; Nanos; Oct4; PGC; Vasa