title: Isolation, characterization and bioactivities of a new polysaccharide from Annona squamosa and its sulfated derivative.
abstract: A new water-soluble polysaccharide, designated as ASPW80-1, was first isolated from the fruit pulp of Annona squamosa. The structure of ASPW80-1 was elucidated based on the physicochemical and instrumental analyses. The results indicated that ASPW80-1 was a homogeneous heteropolysaccharide with an average molecular weight of 2.29×10(5)Da. Another novel modified polysaccharide, the sulfated derivative of ASPW80-1 namely as ASPW80-M1, was also synthesized. The ultra-structures of both ASPW80-1 and ASPW80-M1 were further characterized by scanning electron microscopy and atomic force microscopy. The antioxidant assays showed that ASPW80-1 and ASPW80-M1 exhibited DPPH and hydroxyl radicals scavenging activities. The results of immunomodulatory assays in vitro showed that ASPW80-1 and ASPW80-M1 could markedly promote the proliferation of mouse splenocytes. These results proposed that ASPW80-M1 might be proposed to be developed as a potential value-added product with the activities of immunomodulator and free-radical inhibitors.
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China.
authors: Wensong
authors: W
authors: Tu
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China.
authors: Jianhua
authors: J
authors: Zhu
authors: Department of Pharmacology, Jinan University, Guangzhou 510632, China.
authors: Sixue
authors: S
authors: Bi
authors: Department of Pharmacology, Jinan University, Guangzhou 510632, China.
authors: Dehong
authors: D
authors: Chen
authors: Department of Pharmacology, Jinan University, Guangzhou 510632, China. Electronic address: tsly@jnu.edu.cn.
authors: Liyan
authors: L
authors: Song
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China.
authors: Lishan
authors: L
authors: Wang
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China.
authors: Jiachen
authors: J
authors: Zi
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China. Electronic address: tyrm@jnu.edu.cn.
authors: Rongmin
authors: R
authors: Yu
mesh_terms: D000818:Animals; D031041:Annona / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005638:Fruit / Q000737:chemistry; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013431:Sulfates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D013431:Sulfates
keywords: Annona squamosa; Antioxidant activity; Immunomodulatory activity; Polysaccharide; Structural characterization; Sulfated modification