title: MicroRNA-34b mediates hippocampal astrocyte apoptosis in a rat model of recurrent seizures.
abstract: Recurrent convulsions can cause irreversible astrocyte death, impede neuron regeneration, and further aggravate brain damage. MicroRNAs have been revealed as players in the progression of numerous diseases including cancer and Alzheimer's disease. Particularly, microRNA has been found linked to seizure-induced neuronal death. In this study, a rat model of recurrent convulsions induced by flurothyl treatments was utilised to assess the alterations of microRNA expressions in hippocampus tissues. We also applied an in vitro model in which primary astrocytes were exposed to kainic acid to verify the targets of miR-34b-5p identified in the animal model.
  
  
  
  We discovered that miR-34b-5p, a member of the miR-34 family, increased significantly in flurothyl-treated rat hippocampus tissue. More surprisingly, this upregulation occurred concurrently with accumulating astrocyte apoptosis, indicating the involvement of miR-34b-5p in seizures caused astrocyte apoptosis. Results from the in vitro experiments further demonstrated that miR-34b-5p directly targeted Bcl-2 mRNA, translationally repressed Bcl-2 protein, and thus modulated cell apoptosis by influencing Bcl-2, Bax, and Caspase-3.
  
  
  
  Our findings prove microRNAs play a role in mediating recurrent convulsions-induced astrocyte death and further indicate that miR-34b-5p could acts as a regulator for astrocyte apoptosis induced by recurrent seizures.
authors: Department of Pediatrics, The Second Xiangya Hospital of Central South University, 86 Renmin Middle Rd, Changsha, 410011, Hunan, People's Republic of China.
authors: Liqun
authors: L
authors: Liu
authors: Department of Pediatrics, The Second Xiangya Hospital of Central South University, 86 Renmin Middle Rd, Changsha, 410011, Hunan, People's Republic of China.
authors: Lingjuan
authors: L
authors: Liu
authors: Department of Pediatrics, The Second Xiangya Hospital of Central South University, 86 Renmin Middle Rd, Changsha, 410011, Hunan, People's Republic of China.
authors: Jiayun
authors: J
authors: Shi
authors: Department of Pediatrics, The Second Xiangya Hospital of Central South University, 86 Renmin Middle Rd, Changsha, 410011, Hunan, People's Republic of China.
authors: Menglin
authors: M
authors: Tan
authors: Department of Pediatrics, The Second Xiangya Hospital of Central South University, 86 Renmin Middle Rd, Changsha, 410011, Hunan, People's Republic of China.
authors: Jie
authors: J
authors: Xiong
authors: Department of Pediatrics, The Second Xiangya Hospital of Central South University, 86 Renmin Middle Rd, Changsha, 410011, Hunan, People's Republic of China.
authors: Xingfang
authors: X
authors: Li
authors: Department of Pediatrics, The Second Xiangya Hospital of Central South University, 86 Renmin Middle Rd, Changsha, 410011, Hunan, People's Republic of China.
authors: Qingpeng
authors: Q
authors: Hu
authors: Department of Pediatrics, The Second Xiangya Hospital of Central South University, 86 Renmin Middle Rd, Changsha, 410011, Hunan, People's Republic of China.
authors: Zhuwen
authors: Z
authors: Yi
authors: Department of Pediatrics, The Second Xiangya Hospital of Central South University, 86 Renmin Middle Rd, Changsha, 410011, Hunan, People's Republic of China. mda5611@sina.com.
authors: Ding'an
authors: D
authors: Mao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001253:Astrocytes / Q000378:metabolism* / Q000473:pathology; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005481:Flurothyl; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D020287:In Situ Nick-End Labeling; D007608:Kainic Acid; D035683:MicroRNAs / Q000378:metabolism*; D046228:Microarray Analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000378:metabolism* / Q000473:pathology; D059467:Transcriptome; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; C000617196:Bcl2 protein, rat; C560944:MIRN34 microRNA, rat; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D005481:Flurothyl; C505410:Casp3 protein, rat; D053148:Caspase 3; D007608:Kainic Acid
keywords: Astrocytes; Bcl-2; Convulsion; Hippocampus; MiR-34