title: First next-generation sequencing full-genome characterization of a hepatitis C virus genotype 7 divergent subtype.
abstract: We report the near-full-length genome sequence of a hepatitis C virus (HCV) isolate from a man originating from Democratic Republic of Congo, the genotype of which could not be determined by the routinely used sequencing technique. The near-complete genome sequence of this variant BAK1 was obtained by the association of two next-generation sequencing technologies. Evolutionary analysis indicates that this isolate, BAK1, could be the first reported strain belonging to a new HCV-7b subtype. This new subtype has been incorrectly identified as genotype 2 by the Versant HCV Genotype 2.0 assay (LiPA). The requirement of three independent isolates has been filled, and a new subtype can be assigned. More examples of HCV-7 are required to better understand its origin, its pathogenicity and its relationship with genotype 2.
authors: Université Paris Diderot, Pres Sorbone Paris Cité, Microbiology Laboratory, Hôpital Universitaire Saint-Louis, France.
authors: M
authors: M
authors: Salmona
authors: Clinical Investigation Center, Public Health, UJF-CNRS, Centre Hospitalier Universitaire de Grenoble-Alpes, France; TIMC-IMAG Laboratory, Université de Grenoble Alpes, UMR 5525, Saint-Martin-d'Hères, France.
authors: A
authors: A
authors: Caporossi
authors: Nuffield Department of Medicine, University of Oxford, Oxford, UK.
authors: P
authors: P
authors: Simmonds
authors: Hepato-Gastroenterology Department, Centre Hospitalier Universitaire de Grenoble-Alpes, France.
authors: M-A
authors: MA
authors: Thélu
authors: Virology Laboratory, Institut de Biologie et Pathologie, Centre Hospitalier Universitaire de Grenoble-Alpes, France.
authors: K
authors: K
authors: Fusillier
authors: Université Paris Diderot, Pres Sorbone Paris Cité, Microbiology Laboratory, Hôpital Universitaire Saint-Louis, France.
authors: S
authors: S
authors: Mercier-Delarue
authors: Infectious Diseases Department, Hôpital Saint-Louis, Paris, France.
authors: N
authors: N
authors: De Castro
authors: Université Paris Diderot, Pres Sorbone Paris Cité, Microbiology Laboratory, Hôpital Universitaire Saint-Louis, France.
authors: J
authors: J
authors: LeGoff
authors: Université Paris Diderot, Pres Sorbone Paris Cité, Microbiology Laboratory, Hôpital Universitaire Saint-Louis, France.
authors: M-L
authors: ML
authors: Chaix
authors: TIMC-IMAG Laboratory, Université de Grenoble Alpes, UMR 5525, Saint-Martin-d'Hères, France.
authors: O
authors: O
authors: François
authors: Université Paris Diderot, Pres Sorbone Paris Cité, Microbiology Laboratory, Hôpital Universitaire Saint-Louis, France.
authors: F
authors: F
authors: Simon
authors: Virology Laboratory, Institut de Biologie et Pathologie, Centre Hospitalier Universitaire de Grenoble-Alpes, France; IBS, UMR 5075 CEA-CNRS-UGA, Grenoble, France.
authors: P
authors: P
authors: Morand
authors: Virology Laboratory, Institut de Biologie et Pathologie, Centre Hospitalier Universitaire de Grenoble-Alpes, France; IBS, UMR 5075 CEA-CNRS-UGA, Grenoble, France.
authors: S
authors: S
authors: Larrat
authors: Université Paris Diderot, Pres Sorbone Paris Cité, Microbiology Laboratory, Hôpital Universitaire Saint-Louis, France. Electronic address: sarah.maylin@aphp.fr.
authors: S
authors: S
authors: Maylin
mesh_terms: D019143:Evolution, Molecular; D016679:Genome, Viral; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*
keywords: Democratic Republic of Congo; Genotype 7b; Hepatitis C virus; Next-generation sequencing; Polymorphism