title: Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ.
abstract: The multifunctional Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is known to participate in maintenance and switches of smooth muscle cell (SMC) phenotypes. However, which isoform of CaMKII is involved in differentiation of adult mesenchymal stem cells into contractile SMCs remains unclear. In the present study, we detected γ isoform of CaMKII in differentiation of human adipose derived stem cells (hASCs) into SMCs that resulted from treatment with TGF-β1 and BMP4 in combination for 7 days. The results showed that CaMKIIγ increased gradually during differentiation of hASCs as determined by real-time PCR and western blot analysis. The siRNA-mediated knockdown of CaMKIIγ decreased the protein levels and transcriptional levels of smooth muscle contractile markers (a-SMA, SM22a, calponin, and SM-MHC), while CaMKIIγ overexpression increases the transcriptional and protein levels of smooth muscle contractile markers. These results suggested that γ isoform of CaMKII plays a significant role in smooth muscle differentiation of hASCs.
authors: Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China.
authors: Kaisaier
authors: K
authors: Aji
authors: Prevention and Health Care Department, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830063, China.
authors: Munila
authors: M
authors: Maimaijiang
authors: Department of Joint Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China.
authors: Abudusaimi
authors: A
authors: Aimaiti
authors: Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China.
authors: Mulati
authors: M
authors: Rexiati
authors: Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China.
authors: Baihetiya
authors: B
authors: Azhati
authors: Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China.
authors: Hamulati
authors: H
authors: Tusong
authors: Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China; Institute of Medical Science, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China.
authors: Lei
authors: 0000-0003-4468-8989
authors: L
authors: Cui