title: MicroRNA-891b is an independent prognostic factor of pancreatic cancer by targeting Cbl-b to suppress the growth of pancreatic cancer cells.
abstract: Growing evidence has revealed that microRNAs could regulate the proliferation of pancreatic ductal adenocarcinoma (PDAC) cells and predict the prognosis of PDAC. Here the comparative microRNA expression profiles of the good and poor prognosis groups were performed by microRNA microarray. MicroRNA-891b (miR-891b) was screened and validated to be a prognostic predictor of PDAC in the initial group and further evaluated to be an independent predictor for the overall survival of resectable PDACs in an independent cohort. By a series of cellular and animal experiments, as well as clinical specimen analyses, miR-891b was confirmed to target the Cbl-b gene, promot the expression of tumor suppressor p21 protein and inhibit the proliferation of PDAC cells. The results provide a theoretical basis for the study of miR-891b as an independent prognostic predictor of PDAC and the role of miR-891b/Cbl-b pathway in this prediction, as well as the identification of new targets for PDAC.
authors: Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, 110004, China.
authors: Qian
authors: Q
authors: Dong
authors: Department of Medical Oncology, The First Hospital of China Medical University, Shenyang, 110001, China.
authors: Ce
authors: C
authors: Li
authors: Department of Medical Oncology, The First Hospital of China Medical University, Shenyang, 110001, China.
authors: Xiaofang
authors: X
authors: Che
authors: Department of Medical Oncology, The First Hospital of China Medical University, Shenyang, 110001, China.
authors: Jinglei
authors: J
authors: Qu
authors: Department of Medical Oncology, The First Hospital of China Medical University, Shenyang, 110001, China.
authors: Yibo
authors: Y
authors: Fan
authors: Department of Pathology, Shengjing Hospital of China Medical University, Shenyang, 110004, China.
authors: Xiaohan
authors: X
authors: Li
authors: Department of Pathology, Shengjing Hospital of China Medical University, Shenyang, 110004, China.
authors: Yue
authors: Y
authors: Li
authors: Department of Medical Oncology, The Liaoning Provincial Tumor Hospital, Shenyang, 110042, China.
authors: Qian
authors: Q
authors: Wang
authors: Department of Medical Oncology, The First Hospital of China Medical University, Shenyang, 110001, China.
authors: Yunpeng
authors: Y
authors: Liu
authors: Department of Pathology, Shengjing Hospital of China Medical University, Shenyang, 110004, China.
authors: Xianghong
authors: X
authors: Yang
authors: Department of Medical Oncology, The First Hospital of China Medical University, Shenyang, 110001, China.
authors: Xiujuan
authors: X
authors: Qu
mesh_terms: D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D050721:Proto-Oncogene Proteins c-cbl / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D015398:Signal Transduction; D051900:Smad3 Protein / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D035683:MicroRNAs; C497790:SMAD3 protein, human; D051900:Smad3 Protein; C492211:CBLB protein, human; D050721:Proto-Oncogene Proteins c-cbl
keywords: Cbl-b; microRNA-891b (miR-891b); overall survival (OS); pancreatic ductal adenocarcinoma (PDAC); prognosis