title: shRNA mediated knockdown of Nav1.7 in rat dorsal root ganglion attenuates pain following burn injury.
abstract: Abnormal acute pain after burn injury still torments patients severely. In this study, we investigated that one voltage gated sodium channel Nav1.7 plays a vital role in lowering heat pain threshold after burn injury, and the hypothesis that knockdown of Nav1.7 attenuates pain following burn injury.
  
  
  
  Sixty eight adult male Sprague-Dawley rats were divided into 4 treatment groups: (1) sham, which hind paw was put on the room temperature metal plate for 15 s (2) burn model, which hind paw was put on the 85 °C metal plate for 15 s. (3) Burn injury + lentiviral vector -SCN9AsiRNA-GFP (LV- SCN9AsiRNA-GFP group, n = 18), which receive the DRG microinjection of LV- SCN9AsiRNA-GFP on the zero day. (4) Burn injury + lentiviral vector negative control (LV-NC-GFP group, n = 18), which receive the DRG microinjection of empty lentiviral vector on the zero day.
  
  
  
  Both mechanical and heat threshold were measured from day 1 to 21. Meanwhile, expression of sodium channels Nav1.7 in injured dorsal root ganglia were measured on post-operative days 7(POD 7). Rats exhibited decreased thresholds on both mechanical allodynia and thermal withdrawl latency, accompanied by increased Nav1.7 and c-fos expression in dorsal root ganglion (DRG). And knockdown of Nav1.7 in L5DRG led to the attenuation of burn injury-induced mechanical allodynia and thermal hyperalgesia in the rats.
  
  
  
  We provide evidence that shRNA mediated knockdown of Nav1.7 attenuates burn induced pain in rats as well as decreased the activiation of c-fos protein.
authors: Department of Anatomy, School of Basic Medical Sciences, Zhengzhou University, Henan, China.
authors: Weihua
authors: W
authors: Cai
authors: Department of Anatomy, School of Basic Medical Sciences, Zhengzhou University, Henan, China.
authors: Jing
authors: J
authors: Cao
authors: Department of Anatomy, School of Basic Medical Sciences, Zhengzhou University, Henan, China.
authors: Xiuhua
authors: X
authors: Ren
authors: Department of E.N.T, Zhoukou Central Hospital, Henan, China.
authors: Liang
authors: L
authors: Qiao
authors: Department of Anatomy, School of Basic Medical Sciences, Zhengzhou University, Henan, China.
authors: Xuemei
authors: X
authors: Chen
authors: Department of Anatomy, School of Basic Medical Sciences, Zhengzhou University, Henan, China.
authors: Ming
authors: M
authors: Li
authors: Department of Anatomy, School of Basic Medical Sciences, Zhengzhou University, Henan, China. zwd@zzu.edu.cn.
authors: Weidong
authors: 0000-0001-8365-4672
authors: W
authors: Zang
mesh_terms: D000818:Animals; D002056:Burns / Q000150:complications*; D004195:Disease Models, Animal; D005727:Ganglia, Spinal; D055785:Gene Knockdown Techniques; D006930:Hyperalgesia / Q000235:genetics / Q000473:pathology; D008297:Male; D062556:NAV1.7 Voltage-Gated Sodium Channel / Q000235:genetics*; D010146:Pain / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D017288:Pain Threshold; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D062556:NAV1.7 Voltage-Gated Sodium Channel; D034741:RNA, Small Interfering
keywords: Burn injury pain; Dorsal root ganglion; Lentiviral vector; Nav1.7; shRNA