title: In vitrostability evaluation of coated lipase.
abstract: OBJECTIVE
  The study was conducted to evaluate the stability of commercial coated lipase (CT-LIP)in vitro.
  
  
  METHODS
  The capsules were tested under different conditions with a range of temperature, pH, dry heat treatment and steaming treatment, simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) in this work, respectively. Free lipase (uncoated lipase, UC-LIP) was the control group. Lipase relative activities measured in various treatments were used as a reference frame to characterize the stability.
  
  
  RESULTS
  The lipase activities were decreased with increasing temperatures (p<0.05), and there was a markedly decline (p<0.01) in lipase comparative activities of UC-LIP at 80°C compared with CT-LIP group. Higher relative activities of lipase were observed in CT-LIP group compared with the free one under acidic ambient (pH 3 to 7) and an alkaline medium (pH 8 to 12). Residual lipase activities of CT-LIP group were increased (p<0.05) by 5.67% and 35.60% in dry heat and hydrothermal treatments, respectively. The lipase relative activity profile of CT-LIP was raised at first and dropped subsequently (p<0.05) compared with constantly reduced tendency of UC-LIP exposed to both SGF and SIF.
  
  
  CONCLUSIONS
  The results suggest that the CT-LIP possesses relatively higher stability in comparison with the UC-LIPin vitro. The CT-LIP could retain the potential property to provide sustained release of lipase and thus improved its bioavailability in the gastrointestinal tract.
authors: National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou 310058, China.
authors: Lu Jie
authors: LJ
authors: Liu
authors: National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou 310058, China.
authors: Jia
authors: J
authors: Zhu
authors: National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou 310058, China.
authors: Bin
authors: B
authors: Wang
authors: National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou 310058, China.
authors: Chu
authors: C
authors: Cheng
authors: National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou 310058, China.
authors: Yong Jie
authors: YJ
authors: Du
authors: National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou 310058, China.
authors: Min Qi
authors: MQ
authors: Wang
keywords: Coated Lipase; In vitro; Lipase; Relative Activity; Stability