title: Application of compound mixture of caprylic acid, iron and mannan oligosaccharide against Sparicotyle chrysophrii (Monogenea: Polyopisthocotylea) in gilthead sea bream, Sparus aurata.
abstract: We have evaluated the therapeutic effect of a compound mixture of caprylic acid (200 mg/kg fish), organic iron (0.2% of diet) and mannan oligosaccharide (0.4% of diet) in gilthead sea bream, Sparus aurata Linnaeus, infected with Sparicotyle chrysophrii Beneden et Hesse, 1863 in controlled conditions. One hundred and ten reared and S. chrysophrii-free fish (197 g) located in a cement tank were infected by the parasite two weeks following the addition of 150 S. chrysophrii-infected fish (70 g). Growth parameters and gill parasitic load were measured in treated against control fish after a ten-week-period. Differences in final weight, feed conversion ratio, specific growth rate and feed efficiency were not statistically significant between the experimental groups, suggesting no evident effect with respect to fish growth during the study period. Although the prevalence of S. chrysophrii was not affected by the mixture at the end of the experiment, the number of adults and larvae was significantly lower. The mean intensity encompassing the number of adults and larvae was 8.1 in treated vs 17.7 in control fish. Individual comparisons of gill arches showed that the preferred parasitism site for S. chrysophrii it the outermost or fourth gill arch, consistently apparent in fish fed the modified diet and in control fish. In conclusion, the combined application of caprylic acid, organic iron and mannan oligosaccharide can significantly affect the evolution of infection with S. chrysophrii in gilthead sea bream, being capable of reducing adult and larval stages of the monogenean. However, no difference in growth improvement was observed after the trial period, potentially leaving space for further optimisation of the added dietary compounds.
authors: Institute of Marine Biology, Biotechnology & Aquaculture, Hellenic Center for Marine Research, Aghios Kosmas, Hellinikon, Athens, Greece;
authors: George
authors: G
authors: Rigos
authors: Institute of Oceanography and Fisheries, Split, Croatia This article contains supporting information (Tables S1, S2) online at http://folia.paru.cas.cz/suppl/2016-63-027.pdf.
authors: Ivona
authors: I
authors: Mladineo
authors: Institute of Marine Biology, Biotechnology & Aquaculture, Hellenic Center for Marine Research, Aghios Kosmas, Hellinikon, Athens, Greece;
authors: Chrysa
authors: C
authors: Nikoloudaki
authors: Institute of Oceanography and Fisheries, Split, Croatia This article contains supporting information (Tables S1, S2) online at http://folia.paru.cas.cz/suppl/2016-63-027.pdf.
authors: Anamarija
authors: A
authors: Vrbatovic
authors: Institute of Marine Biology, Biotechnology & Aquaculture, Hellenic Center for Marine Research, Aghios Kosmas, Hellinikon, Athens, Greece;
authors: Dimitra
authors: D
authors: Kogiannou
mesh_terms: D000818:Animals; D002210:Caprylates / Q000494:pharmacology*; D019587:Dietary Supplements; D004338:Drug Combinations; D005393:Fish Diseases / Q000188:drug therapy*; D007501:Iron / Q000494:pharmacology*; D008351:Mannans / Q000494:pharmacology; D009844:Oligosaccharides / Q000494:pharmacology*; D021541:Sea Bream / Q000469:parasitology*; D014200:Trematoda / Q000187:drug effects*; D014201:Trematode Infections / Q000188:drug therapy / Q000662:veterinary*
chemical_list: D002210:Caprylates; D004338:Drug Combinations; D008351:Mannans; D009844:Oligosaccharides; D007501:Iron