title: Influence of surface pre-treatment on the cytocompatibility of a novel biodegradable ZnMg alloy.
abstract: Degradable zinc-based alloys with an appropriate corrosion rate are promising materials for the preparation of temporary orthopaedic implants. Previously, we prepared and characterised a novel Zn1.5Mg alloy. This paper is focused on the characterisation of this alloy after a surface pre-treatment, which should mimic processes occurring in vivo. The samples of the Zn1.5Mg alloy were immersed in a simulated body fluid (SBF) at 37°C for 14days in order to form a protective layer of corrosion products. Thereafter, these samples were used for the corrosion rate determination, an indirect in vitro cytotoxicity test, as well as for a direct contact test and were compared with the non-treated samples. The protective layer was characterized by SEM and its chemical composition was determined by EDS and XPS analysis. The corrosion rate was significantly decreased after the pre-incubation. The protective layer of corrosion products was rich in Ca and P. The pre-incubated samples exhibited increased cytocompatibility in the indirect test (metabolic activity of L929 cells was above 70%) and we also observed osteoblast-like cell growth directly on the samples during the contact tests. Thus, the pre-incubation in SBF leading to improved cytocompatibility could represent more appropriate model to in vivo testing.
authors: Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic. Electronic address: eva.jablonska@vscht.cz.
authors: Eva
authors: E
authors: Jablonská
authors: Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic.
authors: Dalibor
authors: D
authors: Vojtěch
authors: Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic.
authors: Michaela
authors: M
authors: Fousová
authors: Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic.
authors: Jiří
authors: J
authors: Kubásek
authors: Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic.
authors: Jan
authors: J
authors: Lipov
authors: Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic.
authors: Jaroslav
authors: J
authors: Fojt
authors: Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic.
authors: Tomáš
authors: T
authors: Ruml
mesh_terms: D020341:Absorbable Implants; D000497:Alloys / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003343:Corrosion; D008274:Magnesium / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000497:Alloys; D008274:Magnesium; D015032:Zinc
keywords: Biodegradable alloys; Cytocompatibility; In vitro testing; Zinc