title: Flavihumibacter stibioxidans sp. nov., an antimony-oxidizing bacterium isolated from antimony mine soil.
abstract: A Gram-stain-positive, strictly aerobic, non-motile and rod-shaped bacterial strain, designated YS-17T, was isolated from soil in the Lengshuijiang antimony mine, Hunan Province, China. Comparative 16S rRNA gene sequencing analysis clustered it with Flavihumibacter strains, and strain YS-17T was most closely related to Flavihumibacter cheonanensis WS16T (97.2 % similarity), Flavihumibacter petaseus T41T (96.6 %) and Flavihumibacter solisilvae 3-3T (96.5 %). The level of DNA-DNA relatedness between strain YS-17T and F. cheonanensis JCM 19322T was 35.5±0.1 % (n=2). The major respiratory quinone of strain YS-17T was menaquinone-7 and the major polar lipids were phosphatidylethanolamine, two unidentified lipids, two unidentified amino lipids and phospholipid. The major fatty acids (≥5 %) were iso-C15 : 0, iso-C15 : 1 G, unknown ECL 13.565, iso-C17 : 0 3-OH, C15 : 0, C16 : 1ω5c and anteiso-C15 : 0. The DNA G+C content was 47.8 mol%. Compared with other Flavihumibacter strains, strain YS-17T showed major biophysical and biochemical differences, with the ability to hydrolyse gelatin and to assimilate salicin and l-proline. The results demonstrated that strain YS-17T belongs to the genus Flavihumibacter and represents a novel species, for which the name Flavihumibacter stibioxidans sp. nov. is proposed. The type strain is YS-17T (=CCTCC AB 2016053T=KCTC 52205T).
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors: Yushan
authors: Y
authors: Han
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors: Fujun
authors: F
authors: Zhang
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors: Qian
authors: Q
authors: Wang
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors: Shixue
authors: S
authors: Zheng
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors: Wei
authors: W
authors: Guo
authors: School of Environmental Studies, China University of Geosciences, Wuhan, Hubei 430074, PR China.
authors: Liang
authors: L
authors: Feng
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors: Gejiao
authors: G
authors: Wang
mesh_terms: D000965:Antimony; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008906:Mining; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D024482:Vitamin K 2; C062629:menaquinone 7; D000965:Antimony