title: Evaluation of antimicrobial activity of Italian honey for wound healing application in veterinary medicine.
abstract: Honey as a topical treatment for infected wounds dates back to ancient times. However, few studies have been reported concerning the medical properties of Italian honey. In this study, the microbial contamination, the antimicrobial activity and the antibiotic residues of 6 different varieties of Piedmont honeys were evaluated. The antimicrobial activity of honeys was tested by agar well diffusion method and 1 honey for each variety has been selected and tested by broth micro-dilution test to determine Minimum Inhibitory Concentrations (MICs) and evaluated by Minimum Bactericidal Concentrations (MBCs). The honeys with a high level of antibacterial activity were analyzed for the presence of tetracyclines, sulfonamides and macrolide residues. The agar well diffusion method showed the greatest antimicrobial activity for honeydew, chestnut and lime tree honeys. The MICs and MBCs identified the close similarity to the medical manuka honey of honeydew, polyfloral and chestnut honey. The levels of antibiotic residues on these honeys were below the limit of quantification. Based on our results the Italian variety of honeydew showed the best antimicrobial activity and can be considered for the treatment of infected wounds in animals.
authors: Department of Veterinary Sciences, University of Turin, Italy.
authors: E
authors: E
authors: Grego
authors: Department of Veterinary Sciences, University of Turin, Italy.
authors: P
authors: P
authors: Robino
authors: Department of Veterinary Sciences, University of Turin, Italy.
authors: C
authors: C
authors: Tramuta
authors: Department of Veterinary Sciences, University of Turin, Italy.
authors: G
authors: G
authors: Giusto
authors: Council for agricultural research and the analysis of Agrarian Economy, Beekeeping and Silkworm Research Unit (CRA-API), Bologna, Italy.
authors: M
authors: M
authors: Boi
authors: Council for agricultural research and the analysis of Agrarian Economy, Beekeeping and Silkworm Research Unit (CRA-API), Bologna, Italy.
authors: R
authors: R
authors: Colombo
authors: Council for agricultural research and the analysis of Agrarian Economy, Beekeeping and Silkworm Research Unit (CRA-API), Bologna, Italy.
authors: G
authors: G
authors: Serra
authors: Agripiemonte Miele, Torino, Italy.
authors: S
authors: S
authors: Chiadò-Cutin
authors: Department of Veterinary Sciences, University of Turin, Italy.
authors: M
authors: M
authors: Gandini
authors: Department of Veterinary Sciences, University of Turin, Italy.
authors: P
authors: P
authors: Nebbia
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D006722:Honey / Q000032:analysis*; D007558:Italy; D008826:Microbial Sensitivity Tests; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000628:therapy / Q000662:veterinary*
chemical_list: D000890:Anti-Infective Agents
keywords: Antimikrobielle Aktivität; Italian honey; Italienischer Honig; MCB; MIC; Manuka-Honig; antibacterial activity; manuka honey