title: Colorimetric Coupled Enzyme Assay for Cystathionine β-Synthase.
abstract: A colorimetric coupled enzyme assay for the determination of cystathionine β-synthase activity is described. The method exploits cystathionine γ-lyase as an ancillary enzyme capable of transforming cystathionine, produced by cystathionine β-synthase, into cysteine. The cysteine is then spectrophotometrically detected at 560 nm, after its specific complexation with ninhydrin. This method was used to detect cystathionine β-synthase in crude extracts, and for the kinetic characterization of the enzyme partially purified from bovine kidney. A rapid two-step protocol is described for the partial purification of cystathionine γ-lyase from bovine kidney, aimed at a suitable and stable ancillary enzyme preparation.
authors: University of Pisa, Department of Biology, Biochemistry Unit.
authors: Marco
authors: M
authors: Rocchiccioli
authors: Roberta
authors: R
authors: Moschini
authors: Laura
authors: L
authors: Cappiello
authors: Francesco
authors: F
authors: Balestri
authors: Mario
authors: M
authors: Cappiello
authors: Umberto
authors: U
authors: Mura
authors: Antonella
authors: A
authors: Del-Corso
mesh_terms: D000818:Animals; D002417:Cattle; D003124:Colorimetry / Q000379:methods*; D003541:Cystathionine beta-Synthase / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D007700:Kinetics
chemical_list: D003541:Cystathionine beta-Synthase