title: Metabolomics driven analysis of Erythrina lysistemon cell suspension culture in response to methyl jasmonate elicitation.
abstract: An MS-based metabolomic approach was used to profile the secondary metabolite of the ornamental plant Erythrina lysistemon via ultra-performance liquid chromatography coupled to photodiode array detection and high resolution q-TOF mass spectrometry (UPLC-PDA-MS). Cultures maintained the capacity to produce E. lysistemon flavonoid subclasses with pterocarpans amounting for the most abundant ones suggesting that it could provide a resource of such flavonoid subclass. In contrast, alkaloids, major constituents of Erythrina genus, were detected at trace levels in suspension cultures. Methyl jasmonate (MeJA), phytohormone, was further supplied to culture with the aim of increasing secondary metabolites production and with metabolite profiles subjected to multivariate data analysis to evaluate its effect. Results revealed that triterpene i.e. oleanolic acid and fatty acid i.e. hydroxy-octadecadienoic acid were elicited in response to methyl jasmonate, whereas pterocarpans i.e., isoneorautenol showed a decline in response to elicitation suggesting for the induction of terpenoid biosynthetic pathway and concurrent with a down regulation of pterocarpans. In conclusion, a total of 53 secondary metabolites including 3 flavones, 12 isoflavones, 4 isoflavanones, 4 alkaloids, 11 pterocarpans, and 5 phenolic acids were identified.
authors: Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr El-Einy Street, 11562 Cairo, Egypt.
authors: Mohamed A
authors: MA
authors: Farag
authors: Pharmacognosy Department, Faculty of Pharmacy, Alexandria University, El Khartoum Square, 21521 Alexandria, Egypt.
authors: Hattem
authors: H
authors: Mekky
authors: Pharmacognosy Department, Faculty of Pharmacy, Alexandria University, El Khartoum Square, 21521 Alexandria, Egypt.
authors: Sawsan
authors: S
authors: El-Masry
keywords: Cell culture; Erythrina lysistemon; Metabolomics; Methyl jasmonate; Oleanolic acid; Ultra-performance liquid chromatography