title: NFL-lipid nanocapsules for brain neural stem cell targeting in vitro and in vivo.
abstract: The replacement of injured neurons by the selective stimulation of neural stem cells in situ represents a potential therapeutic strategy for the treatment of neurodegenerative diseases. The peptide NFL-TBS.40-63 showed specific interactions towards neural stem cells of the subventricular zone. The aim of our work was to produce a NFL-based drug delivery system able to target neural stem cells through the selective affinity between the peptide and these cells. NFL-TBS.40-63 (NFL) was adsorbed on lipid nanocapsules (LNC) whom targeting efficiency was evaluated on neural stem cells from the subventricular zone (brain) and from the central canal (spinal cord). NFL-LNC were incubated with primary neural stem cells in vitro or injected in vivo in adult rat brain (right lateral ventricle) or spinal cord (T10). NFL-LNC interactions with neural stem cells were different depending on the origin of the cells. NFL-LNC showed a preferential uptake by neural stem cells from the brain, while they did not interact with neural stem cells from the spinal cord. The results obtained in vivo correlate with the results observed in vitro, demonstrating that NFL-LNC represent a promising therapeutic strategy to selectively deliver bioactive molecules to brain neural stem cells.
authors: Université d'Angers, Laboratoire de Neurobiologie et Transgénèse, LNBT, Institut de Biologie en Santé PBH-IRIS, 49033 Angers, France; Université d'Angers, Unité Micro et Nanomédecines Biomimétiques, MINT, Institut de Biologie en Santé PBH-IRIS, 49033 Angers, France; Université catholique de Louvain, Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials, Avenue E. Mounier 73, 1200 Brussels, Belgium.
authors: Dario
authors: D
authors: Carradori
authors: Université d'Angers, Unité Micro et Nanomédecines Biomimétiques, MINT, Institut de Biologie en Santé PBH-IRIS, 49033 Angers, France.
authors: Patrick
authors: P
authors: Saulnier
authors: Université catholique de Louvain, Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials, Avenue E. Mounier 73, 1200 Brussels, Belgium.
authors: Véronique
authors: V
authors: Préat
authors: Université catholique de Louvain, Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials, Avenue E. Mounier 73, 1200 Brussels, Belgium; Université catholique de Louvain, Institute of Materials and Condensed Matter, 1348 Louvain-la-Neuve, Belgium.
authors: Anne
authors: A
authors: des Rieux
authors: Université d'Angers, Laboratoire de Neurobiologie et Transgénèse, LNBT, Institut de Biologie en Santé PBH-IRIS, 49033 Angers, France. Electronic address: joel.eyer@univ-angers.fr.
authors: Joel
authors: J
authors: Eyer
mesh_terms: D000327:Adsorption; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism*; D016503:Drug Delivery Systems; D005260:Female; D053769:Nanocapsules / Q000737:chemistry; D058953:Neural Stem Cells / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism
chemical_list: D004337:Drug Carriers; C576937:NFL-TBS.40-63 peptide; D053769:Nanocapsules; D016900:Neurofilament Proteins; D010446:Peptide Fragments
keywords: Brain subventricular zone; Lipid nanocapsules; NFL-TBS.40-63; Neural stem cells; Spinal cord