title: Production and Purification of Polyclonal Antibodies.
abstract: Polyclonal antibodies consist of a mixture of antibodies produced by multiple B-cell clones that have differentiated into antibody-producing plasma cells in response to an immunogen. Polyclonal antibodies raised against an antigen recognize multiple epitopes on a target molecule, which results in a signal amplification in indirect immunoassays including immune-electron microscopy. In this chapter, we present a basic procedure to generate polyclonal antibodies in rabbits.
authors: Department of Applied Biological Chemistry, Osaka Prefecture University, 1-1, Gakuen-cho, Naka-Ku, Sakai, Osaka, 599-8531, Japan. mami@biochem.osakafu-u.ac.jp.
authors: Masami
authors: M
authors: Nakazawa
authors: Department of Applied Biological Chemistry, Osaka Prefecture University, 1-1, Gakuen-cho, Naka-Ku, Sakai, Osaka, 599-8531, Japan.
authors: Mari
authors: M
authors: Mukumoto
authors: Department of Applied Biological Chemistry, Osaka Prefecture University, 1-1, Gakuen-cho, Naka-Ku, Sakai, Osaka, 599-8531, Japan.
authors: Kazutaka
authors: K
authors: Miyatake
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D000906:Antibodies / Q000097:blood / Q000302:isolation & purification*; D000917:Antibody Formation; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D002454:Cell Differentiation; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D005620:Freund's Adjuvant / Q000008:administration & dosage; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology; D011817:Rabbits
chemical_list: D000906:Antibodies; D000941:Antigens; D000939:Epitopes; D005620:Freund's Adjuvant; D000645:Ammonium Sulfate
keywords: Ammonium sulfate precipitation; Cardiac puncture; Polyclonal antibody; Rabbit; Subcutaneous injection