title: Enhancing biosensor properties of conducting polymers via copolymerization: Synthesis of EDOT-substituted bis(2-pyridylimino)isoindolato-palladium complex and electrochemical sensing of glucose by its copolymerized film.
abstract: 1,3-Bis(2-pyridylimino)isoindoline derivative bearing 3,4-ethylenedioxythiophene (EDOT-BPI) and its palladium complex (EDOT-PdBPI) were synthesized and characterized by FT-IR,1H NMR,13C NMR, UV-Vis spectroscopies and via mass spectrometric analysis. Polymerization of EDOT-PdBPI and copolymerization with 4-amino-N-(2,5-di(thiophene-2-yl)-1H-pyrrol-1-yl)benzamide (HKCN) were carried out by an electrochemical method. In addition, P(EDOT-PdBPI-co-HKCN) modified graphite rod electrode was improved for amperometric glucose sensor based on glucose oxidase (GOx). In this novel biosensor matrix, amino groups in HKCN were used for the enzyme immobilization. On the other hand, EDOT-PdBPI used to mediate the bioelectrocatalytic reaction. Amperometric detection was carried out following oxygen consumption at -0.7V vs. the Ag reference electrode in phosphate buffer (50mM, pH 6.0). The novel biosensor showed a linear amperometric response for glucose within a concentration range of 0.25mM to 2.5mM (LOD: 0.176mM). Amperometric signals at 1mM of glucose were 17.9μA under anaerobic conditions. Amperometric response of the P(EDOT-PdBPI-co-HKCN)/GOx electrode decreased only by 13% within eight weeks. The P(EDOT-PdBPI-co-HKCN)/GOx electrode showed good selectivity in the presence of ethanol and phenol. This result shows that, modification of the proposed biosensor by copolymerization of amine functionalized monomer, which is indispensable to the enzyme immobilization, with palladium complex bearing monomer, which is mediate the bioelectrocatalytic reaction, have provided to give perfect response to different glucose concentrations.
authors: Department of Chemistry, Yıldız Technical University, Davutpaşa, İstanbul, 34210 Turkey.
authors: Tuğçe Yazıcı
authors: TY
authors: Tekbaşoğlu
authors: Department of Chemistry, Pamukkale University, Denizli, 20017 Turkey.
authors: Tugba
authors: T
authors: Soganci
authors: Department of Chemistry, Pamukkale University, Denizli, 20017 Turkey. Electronic address: metinak@pau.edu.tr.
authors: Metin
authors: M
authors: Ak
authors: Department of Chemical Engineering, Marmara University, Kadıköy, İstanbul, 34722 Turkey.
authors: Atıf
authors: A
authors: Koca
authors: Department of Chemistry, Yıldız Technical University, Davutpaşa, İstanbul, 34210 Turkey. Electronic address: mkasimsener@gmail.com.
authors: M Kasım
authors: MK
authors: Şener
mesh_terms: D001230:Aspergillus / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002253:Carbonated Beverages / Q000032:analysis; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D004553:Electric Conductivity; D055664:Electrochemical Techniques / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D000067030:Fruit and Vegetable Juices / Q000032:analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D008958:Models, Molecular; D010165:Palladium / Q000737:chemistry*; D058105:Polymerization; D013876:Thiophenes / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: C000601652:3,4-ethylenedioxythiophene; D056831:Coordination Complexes; D004800:Enzymes, Immobilized; D013876:Thiophenes; D010165:Palladium; D005949:Glucose Oxidase; D005947:Glucose
keywords: 1,3-bis(2-pyridylimino)isoindoline; Conducting polymer; Electropolymerization; Glucose; Glucose biosensors; Palladium