title: Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering.
abstract: Most bacteria in nature exist as biofilms, which support intercellular signalling processes such as quorum sensing (QS), a cell-to-cell communication mechanism that allows bacteria to monitor and respond to cell density and changes in the environment. As QS and biofilms are involved in the ability of bacteria to cause disease, there is a need for the development of methods for the non-invasive analysis of QS in natural bacterial populations. Here, by using surface-enhanced resonance Raman scattering spectroscopy, we report rationally designed nanostructured plasmonic substrates for the in situ, label-free detection of a QS signalling metabolite in growing Pseudomonas aeruginosa biofilms and microcolonies. The in situ, non-invasive plasmonic imaging of QS in biofilms provides a powerful analytical approach for studying intercellular communication on the basis of secreted molecules as signals.
authors: Departamento de Química Física, Universidade de Vigo, 36310 Vigo, Spain.
authors: Gustavo
authors: G
authors: Bodelón
authors: Departamento de Química Física, Universidade de Vigo, 36310 Vigo, Spain.
authors: Verónica
authors: V
authors: Montes-García
authors: Departamento de Química Física, Universidade de Vigo, 36310 Vigo, Spain.
authors: Vanesa
authors: V
authors: López-Puente
authors: Bionanoplasmonics Laboratory, CIC biomaGUNE, Paseo de Miramón 182, 20009 Donostia-San Sebastián, Spain.
authors: Eric H
authors: EH
authors: Hill
authors: Bionanoplasmonics Laboratory, CIC biomaGUNE, Paseo de Miramón 182, 20009 Donostia-San Sebastián, Spain.
authors: Cyrille
authors: C
authors: Hamon
authors: Bionanoplasmonics Laboratory, CIC biomaGUNE, Paseo de Miramón 182, 20009 Donostia-San Sebastián, Spain.
authors: Marta N
authors: MN
authors: Sanz-Ortiz
authors: Departamento de Química Física, Universidade de Vigo, 36310 Vigo, Spain.
authors: Sergio
authors: S
authors: Rodal-Cedeira
authors: Departamento de Química Física, Universidade de Vigo, 36310 Vigo, Spain.
authors: Celina
authors: C
authors: Costas
authors: Departamento de Química Física, Universidade de Vigo, 36310 Vigo, Spain.
authors: Sirin
authors: S
authors: Celiksoy
authors: Departamento de Química Física, Universidade de Vigo, 36310 Vigo, Spain.
authors: Ignacio
authors: I
authors: Pérez-Juste
authors: Bionanoplasmonics Laboratory, CIC biomaGUNE, Paseo de Miramón 182, 20009 Donostia-San Sebastián, Spain.
authors: Leonardo
authors: L
authors: Scarabelli
authors: Bionanoplasmonics Laboratory, CIC biomaGUNE, Paseo de Miramón 182, 20009 Donostia-San Sebastián, Spain.
authors: Andrea
authors: A
authors: La Porta
authors: Departamento de Química Física, Universidade de Vigo, 36310 Vigo, Spain.
authors: Jorge
authors: J
authors: Pérez-Juste
authors: Departamento de Química Física, Universidade de Vigo, 36310 Vigo, Spain.
authors: Isabel
authors: I
authors: Pastoriza-Santos
authors: Departamento de Química Física, Universidade de Vigo, 36310 Vigo, Spain.|Bionanoplasmonics Laboratory, CIC biomaGUNE, Paseo de Miramón 182, 20009 Donostia-San Sebastián, Spain.|Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 20009 Donostia - San Sebastián, Spain.
authors: Luis M
authors: LM
authors: Liz-Marzán
mesh_terms: D018441:Biofilms; D057054:Molecular Imaging; D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000502:physiology*; D053038:Quorum Sensing; D013059:Spectrum Analysis, Raman