title: Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homoeologous Chromosomes.
abstract: Tyramide signal amplification (TSA) fluorescence in situ hybridization (FISH) has been shown as a valuable molecular tool for visualizing specific amplified DNA sequences in chromosome preparations. This chapter describes how to perform TSA-FISH, paying special interest to its two critical steps: probe generation and metaphase plate generation. The potential of physically mapping 12S-globulin sequences by TSA-FISH as a means of identifying homeology among chromosome regions of Avena species was tested and is discussed.
authors: Department of Biomedicine and Biotechnology, University of Alcalá, Campus Universitario, Ctra Madrid-Barcelona Km 33,600, 28871, Alcalá de Henares, Madrid, Spain. araceli.fominaya@uah.es.
authors: Araceli
authors: A
authors: Fominaya
authors: Department of Biomedicine and Biotechnology, University of Alcalá, Campus Universitario, Ctra Madrid-Barcelona Km 33,600, 28871, Alcalá de Henares, Madrid, Spain.
authors: Yolanda
authors: Y
authors: Loarce
authors: Department of Biomedicine and Biotechnology, University of Alcalá, Campus Universitario, Ctra Madrid-Barcelona Km 33,600, 28871, Alcalá de Henares, Madrid, Spain.
authors: Juan M
authors: JM
authors: González
authors: Department of Biomedicine and Biotechnology, University of Alcalá, Campus Universitario, Ctra Madrid-Barcelona Km 33,600, 28871, Alcalá de Henares, Madrid, Spain.
authors: Esther
authors: E
authors: Ferrer
mesh_terms: D000577:Amides / Q000737:chemistry; D018554:Avena / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant; D005456:Fluorescent Dyes / Q000737:chemistry; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D014439:Tyramine / Q000737:chemistry
chemical_list: D000577:Amides; D005456:Fluorescent Dyes; D014439:Tyramine
keywords: Avena; Metaphase chromosomes; Obtaining probes; Physical mapping; TSA-FISH