title: Effect of cigarette smoke on monocyte procoagulant activity: Focus on platelet-derived brain-derived neurotrophic factor (BDNF).
abstract: Cigarette smoke (CS) activates platelets, promotes vascular dysfunction, and enhances Tissue Factor (TF) expression in blood monocytes favoring pro-thrombotic states. Brain-derived neurotrophic factor (BDNF), a member of the family of neurotrophins involved in survival, growth, and maturation of neurons, is released by activated platelets (APLTs) and plays a role in the cardiovascular system. The effect of CS on circulating levels of BDNF is controversial and the function of circulating BDNF in atherothrombosis is not fully understood. Here, we have shown that human platelets, treated with an aqueous extract of CS (CSE), released BDNF in a dose-dependent manner. In addition, incubation of human monocytes with BDNF or with the supernatant of platelets activated with CSE increased TF activity by a Tropomyosin receptor kinase B (TrkB)-dependent mechanism. Finally, comparing serum and plasma samples of 12 male never smokers (NS) and 29 male active smokers (AS) we observed a significant increase in microparticle-associated TF activity (MP-TF) as well as BDNF in AS, while in serum, BDNF behaved oppositely. Taken together these findings suggest that platelet-derived BDNF is involved in the regulation of TF activity and that CS plays a role in this pathway by favoring a pro-atherothrombotic state.
authors: a Centro Cardiologico Monzino , IRCCS , Milan , Italy.
authors: Patrizia
authors: P
authors: Amadio
authors: a Centro Cardiologico Monzino , IRCCS , Milan , Italy.|b Department of Pharmacological and Biomolecular Sciences , University of Milan , Milan , Italy.
authors: Damiano
authors: D
authors: Baldassarre
authors: a Centro Cardiologico Monzino , IRCCS , Milan , Italy.
authors: Leonardo
authors: L
authors: Sandrini
authors: c Division of Hematology-Medical Oncology , Weill Cornell Medical College , New York , NY , USA.
authors: Babette B
authors: BB
authors: Weksler
authors: a Centro Cardiologico Monzino , IRCCS , Milan , Italy.
authors: Elena
authors: E
authors: Tremoli
authors: a Centro Cardiologico Monzino , IRCCS , Milan , Italy.
authors: Silvia S
authors: SS
authors: Barbieri
mesh_terms: D000328:Adult; D000368:Aged; D001779:Blood Coagulation Factors; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism* / Q000494:pharmacology; D016022:Case-Control Studies; D055252:Cell-Derived Microparticles / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D012907:Smoking / Q000009:adverse effects*; D013925:Thromboplastin / Q000378:metabolism
chemical_list: D001779:Blood Coagulation Factors; D019208:Brain-Derived Neurotrophic Factor; C045071:leukocyte procoagulant activity; D013925:Thromboplastin
keywords: BDNF; TF; monocytes; platelets