title: Real-time and in situ enzyme inhibition assay for the flux of hydrogen sulfide based on 3D interwoven AuPd-reduced graphene oxide network.
abstract: A highly sensitive enzyme inhibition analytical platform was established firstly based on paper-supported 3D interwoven AuPd-reduced graphene oxide (rGO) network (NW) for real-time and in situ analysis of H2S released from cancer cells. The novel paper working electrode (PWE) with large electric conductivity, effective surface area and unusual biocompatibility, was fabricated via controllably assembling rGO and AuPd alloy nanoparticles onto the surface of cellulose fibers and into the macropores of paper, which was employed as affinity matrix for horseradish peroxidase (HRP) loading and cells capture. It was the superior performances of AuPd-rGO-NW-PWE that made the loaded HRP exhibit excellent electrocatalytic behavior to H2O2, bring the rapid enhancement of current response. After releasing H2S, the current response would be obviously decreased due to the efficient inhibition effect of H2S on HRP activity. The inhibition degree of HRP was directly proportional to the amount of H2S, and so, the flux of H2S released from cells could be recorded availably. Thus, this proposed enzyme inhibition cyto-sensor could be applied for efficient recording of the release of H2S, which had potential utility to cellular biology and pathophysiology.
authors: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Hongmei
authors: H
authors: Yang
authors: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Yan
authors: Y
authors: Zhang
authors: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Li
authors: L
authors: Li
authors: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Guoqiang
authors: G
authors: Sun
authors: Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, China.
authors: Lina
authors: L
authors: Zhang
authors: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China; Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, China.
authors: Shenguang
authors: S
authors: Ge
authors: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: ujn.yujh@gmail.com.
authors: Jinghua
authors: J
authors: Yu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors / Q000378:metabolism; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000378:metabolism; D061986:MCF-7 Cells; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*; D010209:Paper
chemical_list: D004800:Enzymes, Immobilized; D010087:Oxides; D010165:Palladium; D006046:Gold; D006108:Graphite; D006735:Horseradish Peroxidase; D006862:Hydrogen Sulfide
keywords: 3D interwoven network; AuPd-reduced graphene oxide; Enzyme inhibition cyto-sensor; H(2)S