title: Characterization of a novel cell wall binding domain-containing Staphylococcus aureus endolysin LysSA97.
abstract: Endolysin from Staphylococcus aureus phage SA97 (LysSA97) was cloned and investigated. LysSA97 specifically lyse the staphylococcal strains and effectively disrupted staphylococcal biofilms. Bioinformatic analysis of LysSA97 revealed a novel putative cell wall binding domain (CBD) as well as two enzymatically active domains (EADs) containing cysteine, histidine-dependent amidohydrolases/peptidases (CHAP, PF05257) and N-acetylmuramoyl-L-alanine amidase (Amidase-3, PF01520) domains. Comparison of 98 endolysin genes of S. aureus phages deposited in GenBank showed that they can be classified into six groups based on their domain composition. Interestingly, approximately 80.61 % of the staphylococcal endolysins have a src-homology 3 (SH3, PF08460) domain as CBD, but the remaining 19.39 %, including LysSA97, has a putative C-terminal CBD with no homology to the known CBD. The fusion protein containing green fluorescent protein and the putative CBD of LysSA97 showed a specific binding spectrum against staphylococcal cells comparable to SH3 domain (PF08460), suggesting that the C-terminal domain of LysSA97 is a novel CBD of staphylococcal endolysins.
authors: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, South Korea.
authors: Yoonjee
authors: Y
authors: Chang
authors: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, South Korea. sangryu@snu.ac.kr.|Institute of Food Industrialization, Institutes of Green Bio Science & Technology, Seoul National University, Pyeongchang, Gangwon, 232-916, South Korea. sangryu@snu.ac.kr.
authors: Sangryeol
authors: S
authors: Ryu
mesh_terms: D001665:Binding Sites; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002473:Cell Wall / Q000378:metabolism*; D003001:Cloning, Molecular; D019295:Computational Biology; D010450:Endopeptidases / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D000072417:Protein Domains; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013204:Staphylococcus Phages / Q000201:enzymology* / Q000235:genetics*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology / Q000821:virology*
chemical_list: D010450:Endopeptidases; C001143:endolysin
keywords: Antibacterial; Cell wall binding domain; Endolysin; Staphylococcus aureus