title: Dihydromyricetin Ameliorates 3NP-induced Behavioral Deficits and Striatal Injury in Rats.
abstract: Oxidative stress is closely involved in neurodegenerative diseases. The present study aimed to examine the effect of anti-oxidant DHM (dihydromyricetin) on 3NP (3-nitropropionic acid) -induced behavioral deficits of experimental rats and striatal histopathological injury by using behavioral, imaging, biochemistry, histochemistry and molecular biology technologies. The experimental results showed that both motor dysfunctions and learning and memory impairments induced by 3NP were significantly reduced after DHM treatment. 3NP-induced striatal metabolic abnormality was also remarkably improved by DHM treatment, showed as the increased glucose metabolism in PET/CT scan, decreased MDA (malondialdehyde) and increased SOD (superoxide dismutase) activity in enzyme histochemical staining. In addition, the cell apoptosis was evidently detected in the striatum of the 3NP group, while in the 3NP + DHM group, the number of apoptotic cells was remarkably reduced. 3NP treatment obviously induced down-regulation of Bcl-2, and up-regulations of Bax and Cleaved Caspase-3, while these changes were significantly reversed by DHM treatment. The present results suggested that DHM showed its protective effect by anti-oxidant and anti-apoptosis mechanisms.
authors: College of Psychology and Sociology, Shenzhen University, Shenzhen, China.
authors: Shuhua
authors: S
authors: Mu
authors: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China.
authors: Youlan
authors: Y
authors: Li
authors: Department of Anesthesiology, Guangdong No. 2 Provincial People's Hospital, Guangdong Provincial Emergency Hospital, Guangzhou, China.
authors: Bingbing
authors: B
authors: Liu
authors: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China.
authors: Weiping
authors: W
authors: Wang
authors: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China.
authors: Si
authors: S
authors: Chen
authors: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China.
authors: Jiajia
authors: J
authors: Wu
authors: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China.
authors: Lisi
authors: L
authors: OuYang
authors: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China.
authors: Yaxi
authors: Y
authors: Zhu
authors: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China.
authors: Keyi
authors: K
authors: Li
authors: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China.
authors: Mali
authors: M
authors: Zhan
authors: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China.
authors: Zongwei
authors: Z
authors: Liu
authors: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China.
authors: Yu
authors: Y
authors: Jia
authors: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China.
authors: Yuxin
authors: Y
authors: Ma
authors: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China. leiwl@mail.sysu.edu.cn.
authors: Wanlong
authors: W
authors: Lei
mesh_terms: D000818:Animals; D017209:Apoptosis; D003342:Corpus Striatum / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism; D044948:Flavonols / Q000494:pharmacology / Q000627:therapeutic use*; D008124:Locomotion; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018782:Maze Learning; D019636:Neurodegenerative Diseases / Q000188:drug therapy* / Q000209:etiology; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D009574:Nitro Compounds / Q000633:toxicity; D018384:Oxidative Stress; D000072078:Positron Emission Tomography Computed Tomography; D011422:Propionates / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D044948:Flavonols; D018696:Neuroprotective Agents; D009574:Nitro Compounds; D011422:Propionates; D008315:Malondialdehyde; D013482:Superoxide Dismutase; C472036:dihydromyricetin; C015392:3-nitropropionic acid
keywords: 3NP; Apoptosis; DHM; Oxidative stress; Striatum