title: Physicochemical properties of peptide-coated microelectrode arrays and their in vitro effects on neuroblast cells.
abstract: Silicon micromachined neural electrode arrays, which act as an interface between bioelectronic devices and neural tissues, play an important role in chronic implants, in vivo. The biological compatibility of chronic microelectrode arrays (MEA) is an essential factor that must be taken into account in their design and fabrication. In order to improve biocompatibility of the MEAs, the surface of the electrodes was coated with polyethylene glycol (PEG) and parylene-C, which are biocompatible polymers. An in vitro study was performed to test the capacity of poly-d-lysine (PDL) to improve neural-cell adhesion and proliferation. Increased proliferation of the neuroblast cells on the microelectrodes was observed in the presence of the PDL. The presence of the peptide on the electrode surface was confirmed using Fourier transform infrared spectroscopy and scanning electron microscopy (SEM). The impedance of the electrodes was not changed significantly before and after PDL deposition. Mouse neuroblast cells were seeded and cultured on the PDL coated and uncoated neural MEAs with different tip-coatings such as platinum, molybdenum, gold, sputtered iridium oxide, and carbon nanotubes. The neuroblast cells grew preferentially on and around peptide coated-microelectrode tips, as compared to the uncoated microelectrodes.
authors: Polystim Neurotechnologies Laboratory, Department of Electrical Engineering, Polytechnique Montreal, QC H3C 3A7, Canada. Electronic address: bahar.ghane@gmail.com.
authors: Bahareh
authors: B
authors: Ghane-Motlagh
authors: Department of Chemistry, University of Montreal, QC H3C 3J7, Canada.
authors: Taraneh
authors: T
authors: Javanbakht
authors: Department of Chemistry, University of Montreal, QC H3C 3J7, Canada.
authors: Fatemeh
authors: F
authors: Shoghi
authors: Department of Chemistry, University of Montreal, QC H3C 3J7, Canada.
authors: Kevin J
authors: KJ
authors: Wilkinson
authors: Department of Chemistry, University of Montreal, QC H3C 3J7, Canada.
authors: Richard
authors: R
authors: Martel
authors: Polystim Neurotechnologies Laboratory, Department of Electrical Engineering, Polytechnique Montreal, QC H3C 3A7, Canada.
authors: Mohamad
authors: M
authors: Sawan
mesh_terms: D000818:Animals; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D008839:Microelectrodes; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D014992:Xylenes / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D010455:Peptides; D011108:Polymers; D014992:Xylenes; D011107:Polylysine; C011055:parylene; D011092:Polyethylene Glycols; C000595209:polyethylene glycol 1000
keywords: FTIR; Hydrogel; Neural microelectrode array; PEG; Peptide