title: A G-quadruplex-selective luminescent probe with an anchor tail for the switch-on detection of thymine DNA glycosylase activity.
abstract: Thymine DNA glycosylase (TDG) performs essential functions in maintaining genetic integrity and epigenetic regulation, which also plays an essential role in DNA demethylation. In this work, the novel iridium(III) complex 1 with an anchor tail was synthesized and employed to construct a G-quadruplex-based assay for detecting TDG activity in aqueous solution by using the mismatched base excising property of TDG with T4 DNA ligase and phi29 DNA polymerase, in concert with the rolling circle amplification (RCA) strategy. The assay achieved a detection limit of 0.048UmL(-)(1) (0.012ngmL(-1)), and showed high selectivity towards TDG even in the presence of other proteins and enzymes. Additionally, the assay could function in diluted cellular debris.
authors: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors: Sheng
authors: S
authors: Lin
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors: Tian-Shu
authors: TS
authors: Kang
authors: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China; College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, China.
authors: Lihua
authors: L
authors: Lu
authors: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors: Wanhe
authors: W
authors: Wang
authors: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. Electronic address: edmondma@hkbu.edu.hk.
authors: Dik-Lung
authors: DL
authors: Ma
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China. Electronic address: duncanleung@umac.mo.
authors: Chung-Hang
authors: CH
authors: Leung
mesh_terms: D015342:DNA Probes / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D054856:G-Quadruplexes; D008163:Luminescent Measurements / Q000295:instrumentation*; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044502:Thymine DNA Glycosylase / Q000032:analysis* / Q000235:genetics*
chemical_list: D015342:DNA Probes; D044502:Thymine DNA Glycosylase
keywords: G-quadruplex; Iridium(III) complex; Luminescence; TDG