title: A redox-mediated chromogenic reaction and application in immunoassay.
abstract: A novel redox-mediated chromogenic reaction was demonstrated based on the reaction between HAuCl4 and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), which generate various color responses from red to green in the resulting solutions. Various redox substance could be used to mediate the reaction and trigger a distinct color response. We established a sensitive hydrogen peroxide colorimetric sensor based on the redox-mediated chromogenic reaction and depicted the application both in detection of enzyme and in an immunoassay. Combining the traditional chromogenic reagent with gold nanoparticles, our assay has the advantage in short response time (within three minutes), high sensitivity (10(-12) g mL(-1) for HBsAg) and stability.
authors: Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors: Ru-Jia
authors: RJ
authors: Yu
authors: Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors: Wei
authors: W
authors: Ma
authors: Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors: Mao-Pan
authors: MP
authors: Peng
authors: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: baizs@ecust.edu.cn.
authors: Zhi-Shan
authors: ZS
authors: Bai
authors: Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: ytlong@ecust.edu.cn.
authors: Yi-Tao
authors: YT
authors: Long
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D002374:Catalase / Q000032:analysis* / Q000378:metabolism; D002712:Chlorides / Q000737:chemistry*; D003124:Colorimetry; D017612:Gold Compounds / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay; D010084:Oxidation-Reduction; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D052160:Benzothiazoles; D002712:Chlorides; D017612:Gold Compounds; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C024568:gold tetrachloride, acid; D006861:Hydrogen Peroxide; D002374:Catalase
keywords: Colorimetric assay; Detection of hydrogen peroxide; Redox-mediated chromogenic reaction; Sandwich ELISA