title: The Arabidopsis hnRNP-Q Protein LIF2 and the PRC1 Subunit LHP1 Function in Concert to Regulate the Transcription of Stress-Responsive Genes.
abstract: LHP1-INTERACTING FACTOR2 (LIF2), a heterogeneous nuclear ribonucleoprotein involved inArabidopsis thalianacell fate and stress responses, interacts with LIKE HETEROCHROMATIN PROTEIN1 (LHP1), a Polycomb Repressive Complex1 subunit. To investigate LIF2-LHP1 functional interplay, we mapped their genome-wide distributions in wild-type,lif2, andlhp1backgrounds, under standard and stress conditions. Interestingly, LHP1-targeted regions form local clusters, suggesting an underlying functional organization of the plant genome. Regions targeted by both LIF2 and LHP1 were enriched in stress-responsive genes, the H2A.Z histone variant, and antagonistic histone marks. We identified specific motifs within the targeted regions, including a G-box-like motif, a GAGA motif, and atelo-box. LIF2 and LHP1 can operate both antagonistically and synergistically. In response to methyl jasmonate treatment, LIF2 was rapidly recruited to chromatin, where it mediated transcriptional gene activation. Thus, LIF2 and LHP1 participate in transcriptional switches in stress-response pathways.
authors: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, F-78000 Versailles, France.
authors: Anne M
authors: AM
authors: Molitor
authors: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, F-78000 Versailles, France.
authors: David
authors: D
authors: Latrasse
authors: URGI, INRA, Université Paris-Saclay, F-78000 Versailles, France.
authors: Matthias
authors: M
authors: Zytnicki
authors: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, F-78000 Versailles, France.|Sorbonne Universités, UPMC Université Paris 06, UFR927, F-75005 Paris, France.
authors: Philippe
authors: http://orcid.org/0000-0001-5932-6863
authors: P
authors: Andrey
authors: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, F-78000 Versailles, France.
authors: Nicole
authors: N
authors: Houba-Hérin
authors: URGI, INRA, Université Paris-Saclay, F-78000 Versailles, France.
authors: Mélanie
authors: http://orcid.org/0000-0003-0040-5959
authors: M
authors: Hachet
authors: CEA-Institut de Génomique, Centre National de Séquençage, F-91057 Evry, France.
authors: Christophe
authors: http://orcid.org/0000-0001-6849-7824
authors: C
authors: Battail
authors: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, F-78000 Versailles, France.
authors: Stefania
authors: S
authors: Del Prete
authors: CEA-Institut de Génomique, Genoscope, Centre National de Séquençage, F-91057 Evry, France.
authors: Adriana
authors: A
authors: Alberti
authors: URGI, INRA, Université Paris-Saclay, F-78000 Versailles, France.
authors: Hadi
authors: http://orcid.org/0000-0003-3001-4908
authors: H
authors: Quesneville
authors: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, F-78000 Versailles, France valerie.gaudin@versailles.inra.fr.
authors: Valérie
authors: http://orcid.org/0000-0002-1356-5567
authors: V
authors: Gaudin