title: PLA-grafting of collagen chains leading to a biomaterial with mechanical performances useful in tendon regeneration.
abstract: With the aim to obtain a scaffold with improved mechanical properties with respect to collagen for tendon augmentation and regeneration, a novel collagen-based material was prepared via heterogeneous phase derivatization of type I collagen sponges using polylactic acid. Compared to the untreated collagen, the functionalized sponge (Coll-PLA) was characterized by higher tensile properties and lower swelling capability; the degradation rate of Coll-PLA, in the presence of collagenase, was lower than that of the untreated collagen sponge. These results are related to an increased hydrophobic character of the collagen matrix due to the presence of PLA chains. In vitro tests, performed with human primary fibroblasts, showed that cell adhesion and proliferation rate on Coll-PLA were comparable to those obtained with the non-functionalized collagen. These findings suggest that the new biomaterial could be suitable as scaffold in tendon augmentation and regeneration.
authors: Novagenit S.r.l, Viale Trento 115/117, 38017 Mezzolombardo (TN), Italy. Electronic address: d.bellini@novagenit.com.
authors: Davide
authors: D
authors: Bellini
authors: Novagenit S.r.l, Viale Trento 115/117, 38017 Mezzolombardo (TN), Italy; Department of Drug Chemistry and Technologies, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy. Electronic address: claudia.cencetti@gmail.com.
authors: Claudia
authors: C
authors: Cencetti
authors: Novagenit S.r.l, Viale Trento 115/117, 38017 Mezzolombardo (TN), Italy. Electronic address: a.sacchetta@novagenit.com.
authors: Anna Cristina
authors: AC
authors: Sacchetta
authors: Novagenit S.r.l, Viale Trento 115/117, 38017 Mezzolombardo (TN), Italy. Electronic address: am.battista@novagenit.com.
authors: Angela Maria
authors: AM
authors: Battista
authors: Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy. Electronic address: andrea.martinelli@uniroma1.it.
authors: Andrea
authors: A
authors: Martinelli
authors: Transfusion Medicine and Regeneration Medicine, Hospital SS Antonio e Biagio, Via Venezia 16, 15121 Alessandria, Italy. Electronic address: lmazzucco@ospedale.al.it.
authors: Laura
authors: L
authors: Mazzucco
authors: Department of Biochemical Sciences, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy. Electronic address: anna.scottodabusco@uniroma1.it.
authors: Anna
authors: A
authors: Scotto D'Abusco
authors: Department of Drug Chemistry and Technologies, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy. Electronic address: pietro.matricardi@uniroma1.it.
authors: Pietro
authors: P
authors: Matricardi
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D011091:Polyesters / Q000737:chemistry; D012038:Regeneration; D013710:Tendons; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C033616:poly(lactide); D003094:Collagen