title: An expedient reverse-phase high-performance chromatography (RP-HPLC) based method for high-throughput analysis of deferoxamine and ferrioxamine in urine.
abstract: The present study was planned to optimize and validate an expedient reverse-phase high chromatography (RP-HPLC) based protocol for the analysis of deferoxamine (DFO) and ferrioxamine (FO) in urinary execration of patients suffering β-thalassemia major. The optimized RP-HPLC method was found to be linear over the wide range of DFO and FO concentration (1-90 μg/mL) with appreciable recovery rates (79.64-97.30%) of quality controls at improved detection and quantitation limits and acceptable inter and intraday variability. Real-time analysis of DFO and FO in the urine of thalassemic patients (male and female) at different intervals of Desferal®(Novartis Pharmaceuticals Corporation) injection revealed DFO and FO excretion at significantly (p < 0) different rates. The maximum concentrations of DFO (76.7 ± 3.06 μg/mL) and FO (74.2 ± 3.25 μg/mL) were found in urine samples, collected after 6 h of drug infusion while the minimum levels of DFO (1.10 ± 0.12 μg/mL) and FO (2.97 ± 0.13 μg/mL) were excreted by patients after 24 h. The present paper offers balanced conditions for an expedient, reliable and quick determination of DFO and FO in urine samples.
authors: Department of BioSciences, COMSATS Institute of Information Technology, Islamabad, Pakistan.|Department of Biochemistry, University of Agriculture, Faisalabad, -38040, Pakistan.
authors: Bushra
authors: B
authors: Arshad
authors: Department of Biochemistry, University of Agriculture, Faisalabad, -38040, Pakistan.
authors: Tahira
authors: T
authors: Iqbal
authors: Department of Chemistry, Kinnaird College University, Lahore, -54000, Pakistan.
authors: Sumia
authors: S
authors: Akram
authors: Department of Chemistry, Government College University, Lahore, -54600, Pakistan.
authors: Muhammad
authors: M
authors: Mushtaq
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D003287:Contrast Media / Q000493:pharmacokinetics*; D003676:Deferoxamine / Q000652:urine*; D005260:Female; D005290:Ferric Compounds / Q000652:urine*; D006801:Humans; D057230:Limit of Detection; D008297:Male; D017262:Siderophores / Q000652:urine*; D017086:beta-Thalassemia / Q000652:urine*
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; D017262:Siderophores; C002577:ferrioxamine B; D003676:Deferoxamine
keywords: DFO and FO; RP-HPLC-DAD; urinary excretion; β-thalassemia major