title: [Determination of HVA and VMA in urine using high performance liquid chromatography-tandem mass spectrometry].
abstract: OBJECTIVE
  To establish a method for the determination of homovanillic acid (HVA) and vanil mandelic acid (VMA) in urine, using high performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS) detection.
  
  
  METHODS
  A 200 μl volume of urine sample added with 20 μl chlorzoxazone internal standard was mixed in a centrifuge tube, then added 100μL methanol (containing 1% acetic acid) , vortex mixed and centrifuged at 13 000 rpm for 1 min. A 10 μl volume of the above supernatant solution was injected into HPLC/MS/MS system for quantitative analysis.
  
  
  RESULTS
  With internal standard method, the calibration curves of HVA and VMA were linear in the range of 20~10 000 ng/ml and 20~7 500 ng/ml, with correlation coefficient of 0.995 6 and 0.997 0, respectively. The LOQ of HVA and VMA was 4.69 ng/ml and 2.33 ng/ml, respectively. The method recoveries were in the range of 85.72%~117.95% for HVA, and 95.82%~113.78% for VMA.
  
  
  CONCLUSIONS
  This method is simple, rapid and characterized with acceptable sensitivity and accuracy to meet the requirements for the analysis of homovanillic acid and vanilla mandelic acid.
authors: Department of Occupational Disease and Toxication, Chaoyang Hospital Affiliated to the Capital University of Medical Science, Beijing 100020, China.
authors: H L
authors: HL
authors: Li
authors: G C
authors: GC
authors: Wang
authors: J
authors: J
authors: Zhu
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006719:Homovanillic Acid; D006801:Humans; D053719:Tandem Mass Spectrometry; D014642:Vanilmandelic Acid
chemical_list: D014642:Vanilmandelic Acid; D006719:Homovanillic Acid