title: PaCaHa inhibits proliferation of human cancer cells in vitro.
abstract: OBJECTIVE
  The aim of this study was to investigate the antiproliferative and cytotoxic effects of a newly synthesized molecule named paracetamol acetohydroxamic acid (PaCaHa) on human neoplastic cell lines.
  
  
  METHODS
  A549, CRL 2923, HeLa, and ARPE were treated with various concentrations of PaCaHa and DMSO (vehicle control). The cytotoxic/cytostatic effects of PaCaHa were determined after a 24-h incubation period and compared to the DMSO control. The cytotoxic and antiproliferative effects were determined by the trypan blue dye exclusion and MTT methods.
  
  
  RESULTS
  A higher susceptibility to PaCaHA was found in CRL 2923 and HeLa cells, while A549 and ARPE cells were less responsive to PaCaHa. The percent of cytotoxicity resulting from 400 µg/mL of PaCaHa were >90 for CRL-2923 and HeLa, 68 for A549, and 64 for ARPE cells. The cytotoxic difference between CRL-2923/HeLa and ARPE/A549 cells was significant (P < 0.05).
  
  
  CONCLUSIONS
  PaCaHa showed dose dependent cytotoxic and antiproliferative effects on three distinct human cancer cell lines. The differential effect of PaCaHa on different cancer cell lines suggests that PaCaHa could have a potential antitumor effect on specific cancer types. These results support further comprehensive studies on PaCaHa and its derivatives.
authors: Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdoğan University, Rize, Turkey.
authors: Saliha
authors: S
authors: Ekşi
authors: Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdoğan University, Rize, Turkey.
authors: Nebahat
authors: N
authors: Ejder
authors: Department of Chemistry, Faculty of Arts and Sciences, Recep Tayyip Erdoğan University, Rize, Turkey.
authors: Fatih
authors: F
authors: Yilmaz
authors: Department of Chemistry, Faculty of Arts and Sciences, Recep Tayyip Erdoğan University, Rize, Turkey.
authors: Ayşe
authors: A
authors: Ertürk
authors: Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdoğan University, Rize, Turkey.
authors: Cemal
authors: C
authors: Sandalli
mesh_terms: D000082:Acetaminophen; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D009369:Neoplasms / Q000473:pathology*
chemical_list: D000082:Acetaminophen
keywords: A549; ARPE-19; CRL-2923; HeLa; MTT; Paracetamol acetohydroxamic acid; cancer