title: Effect of fucoidan from Turbinaria conoides on human lung adenocarcinoma epithelial (A549) cells.
abstract: Fucoidan was purified from seaweed, Turbinaria conoides. Isolated fragments were characterized with NMR ((13)C, (1)H), Gas Chromatography-Mass Spectronomy (GC-MS) and HPLC analysis. The autohydrolysate of fucoidans consisted of sulfated fuco-oligosaccharides having the backbone of α-(1, 3)-linked fuco-pyranose derivatives and minor components of galactose, glucose, mannose and xylose sugars. Fucoidan induced a dose-dependent reduction in cell survival of lung cancer A549 cells by MTT assay (GI50, 75μg/mL). However, it was not cytotoxic to a non-tumorigenic human keratinocyte cell line of skin tissue (HaCaT) (GI50>1.0mg/mL). The apoptotic cells in fucoidan-treated A549 cells were visualized by laser confocal microscopy and cell cycle analysis showed induction of G0/G1 phase arrest of the cell progression cycle. Further, CFSE labeling and flow cytometry highlighted that fucoidan significantly (P<0.05) inhibited the proliferation rate of A549 cells by up to 2-fold compared with the control cells. It is concluded that fucoidan has the potential to act as an anti-proliferative agent on lung carcinoma (A549) cells.
authors: Unit of Aquatic Biodiversity, Department of Zoology, University of Madras, Guindy Campus, Chennai-25, India. Electronic address: madhu.unom5@gmail.com.
authors: Madhavarani
authors: M
authors: Alwarsamy
authors: Faculty of Agriculture and Life Sciences, Lincoln University, PO Box 85084, Lincoln 7647, New Zealand. Electronic address: Ravi.Gooneratne@lincoln.ac.nz.
authors: Ravi
authors: R
authors: Gooneratne
authors: Unit of Aquatic Biodiversity, Department of Zoology, University of Madras, Guindy Campus, Chennai-25, India. Electronic address: rramani8@unom.ac.in.
authors: Ramanibai
authors: R
authors: Ravichandran
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004847:Epithelial Cells / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D000459:Phaeophyceae / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000473:pathology; D012624:Seaweed / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; C007789:fucoidan
keywords: Apoptosis; CFSE labeling; Fucoidan (PubChem CID: 92023653); G0/G1 arrest; Structural analysis