title: In vitro evaluation of the schistosomicidal effect of the extracts, fractions and major 3-hydroxy-2,6-dialkyl-substituted piperidine alkaloids from the flowers of Senna spectabilis (Fabaceae).
abstract: In this work, we present the in vitro schistosomicidal activity evaluation of the most active dichloromethane fraction (FDm) (ED50=83.5μg/mL) and of a mixture of the major alkaloids ((-)-cassine/(-)-spectaline, C/E) (ED50=37.4μg/mL) from the flowers of Senna spectabilis against adult worms and cercariae. We also demonstrate other toxic effects including paralysis of the adult worms, inhibition of the secretory activity, tegument lesions and cercaricidal activity. In the association test of Praziquantel (PZQ)-C/E, we observed up to 80% mortality of Schistosoma mansoni in comparison to PZQ monotherapy. Due to the diversity of the toxic effects, the schistosomicidal activity of C/E is likely a result of a multitarget mechanism involving the tegument, secretory system and neuromotor action.
authors: Universidade Federal de Alfenas Minas Gerais, Alfenas 37130-000, Brazil.
authors: Andreísa Teixeira
authors: AT
authors: de Castro
authors: Universidade Federal de Alfenas Minas Gerais, Alfenas 37130-000, Brazil.
authors: Aline Pereira
authors: AP
authors: Castro
authors: Universidade Federal de Alfenas Minas Gerais, Alfenas 37130-000, Brazil.
authors: Matheus Siqueira
authors: MS
authors: Silva
authors: Universidade Federal de Alfenas Minas Gerais, Alfenas 37130-000, Brazil.
authors: Isabella Maria Monteiro
authors: IM
authors: de Souza
authors: Universidade Federal de Alfenas Minas Gerais, Alfenas 37130-000, Brazil.
authors: Raquel Lopes
authors: RL
authors: Martins-Souza
authors: Universidade Federal de Alfenas Minas Gerais, Alfenas 37130-000, Brazil.
authors: Daniela Aparecida
authors: DA
authors: Chagas-Paula
authors: Universidade Federal de Alfenas Minas Gerais, Alfenas 37130-000, Brazil.
authors: Luiz Felipe Leomil
authors: LF
authors: Coelho
authors: Universidade Estadual Paulista São Paulo, Araraquara 14800-060, Brazil.
authors: Vanderlan
authors: V
authors: da Silva Bolzani
authors: Universidade Federal de Uberlândia Minas Gerais, Uberlândia 38408-100, Brazil.
authors: Marcos
authors: M
authors: Pivatto
authors: Universidade Federal de Alfenas Minas Gerais, Alfenas 37130-000, Brazil.
authors: Claudio
authors: C
authors: Viegas
authors: Universidade Federal de Alfenas Minas Gerais, Alfenas 37130-000, Brazil. Electronic address: marques@unifal-mg.edu.br.
authors: Marcos José
authors: MJ
authors: Marques
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D007887:Fabaceae / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D035264:Flowers / Q000737:chemistry / Q000378:metabolism; D007659:Ketones / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D010880:Piperidines / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry*; D012550:Schistosoma mansoni / Q000187:drug effects; D012556:Schistosomicides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013237:Stereoisomerism
chemical_list: D000470:Alkaloids; D007659:Ketones; D010880:Piperidines; D010936:Plant Extracts; D012556:Schistosomicides; C480880:spectaline; C426663:cassine
keywords: Alkaloids; Fluorescent probes; Schistosomiasis; Senna spectabilis