title: A silver carp skin derived collagen in bone defect treatment-A histological study in a rat model.
abstract: In recent years, there has been increasing interest in elaboration of novel therapeutic strategies, such as the use of the marine collagen products. Biochemical properties of marine collagen are different from those of mammalian collagen; e.g., its extremely high solubility in diluted acid. Extracts produced using low temperature techniques contain a number of small proteins and collagen with preserved triple helix structure. The aim of the study was to evaluate the influence of a new marine product Collgel®obtained with a unique method from a silver carp (Hypophthalmichthys molitrix) on bone defect healing in a rat study. For this purpose bone defects with diameters of 5mm were created in 15 animals and subsequently filled with Collgel combined with another commercially available material. Samples were processed for histological evaluation and a Micro-CT study was performed. Histological analysis showed new bone formation in all groups after 8 weeks. The bone formation was significantly increased in treated bone lesions compared to untreated bone tissue. However no significant difference was noted between the healing of the defects filled with xenogenic bovine derived bone substitute alone and xenogenic, bovine derived bone substitute combined with a marine delivered collagen. Finding from the histological examination was confirmed in a Micro-CT study. The study has shown that the new marine product can be used instead of conventional porcine or bovine collagen membranes in guided bone regeneration.
authors: Department of Dental Surgery, Wroclaw Medical University, Wroclaw, Poland. Electronic address: jakub.hadzik@umed.wroc.pl.
authors: Jakub
authors: J
authors: Hadzik
authors: Department of Dental Surgery, Wroclaw Medical University, Wroclaw, Poland.
authors: Paweł
authors: P
authors: Kubasiewicz-Ross
authors: Department of Orthodontics, Carl Gustav Carus Campus, Technische Universität Dresden, Germany.
authors: Christiane
authors: C
authors: Kunert-Keil
authors: Department of Dental Surgery, Wroclaw Medical University, Wroclaw, Poland.
authors: Kamil
authors: K
authors: Jurczyszyn
authors: Department of Pharmaceutical Biology and Botany, Medical University of Wroclaw, Poland.
authors: Izabela
authors: I
authors: Nawrot-Hadzik
authors: Department of Dental Surgery, Wroclaw Medical University, Wroclaw, Poland.
authors: Marzena
authors: M
authors: Dominiak
authors: Department of Orthodontics, Carl Gustav Carus Campus, Technische Universität Dresden, Germany.
authors: Tomasz
authors: T
authors: Gedrange
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000627:therapeutic use*; D002347:Carps / Q000378:metabolism*; D003094:Collagen / Q000627:therapeutic use*; D005260:Female; D017102:Fracture Healing; D048091:Guided Tissue Regeneration / Q000379:methods; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000378:metabolism*; D012887:Skull Fractures / Q000473:pathology* / Q000628:therapy*; D016896:Treatment Outcome
chemical_list: D018786:Bone Substitutes; D003094:Collagen
keywords: Bone defect; Fish collagen; Guided bone regeneration