title: Loss of MiR-424-3p, not miR-424-5p, confers chemoresistance through targeting YAP1 in non-small cell lung cancer.
abstract: MiR-424 has been discovered to be involved in the chemoresistance of lung cancer. However, the underlying mechanism by which miR-424 played role in chemoresistance has been unknown. Here, in our study, to investigate the role of miR-424 in non-small cell lung cancer (NSCLC), we have detected the expression of miR-424-3p and -5p in NSCLC tissues and paired normal control. Moreover, to explore the role of miR-424-3p in NSCLC cells, miR-424-3p and -5p were both re-expressed and knocked down using transient transfection with their respective mimics and inhibitors. Cell viability, migration, and invasion were evaluated using MTT, wound-healing and Transwell assays, respectively. It was found that down-regulation of miR-424-3p was pronouncedly associated with NSCLC progression and overall prognosis; and that both miR-424-3p and -5p were markedly capable of preventing the proliferation, migration, and invasion in NSCLC cells. Additionally, it is miR-424-3p but not miR-424-5p that enhances the chemo-sensitivity of NSCLC cells through targeting YAP1. Mechanistically, YAP1 was identified as down-stream target of miR-424-3p. Together, it was for the first time in our study found that it is loss of miR-424-3p not miR-424-5p that enables chemoresistance through targeting YAP1 in NSCLC, supporting that miR-424-3p could be used as therapeutic target in the curing of NSCLC with chemoresistance. © 2016 Wiley Periodicals, Inc.
authors: Department of Respiratory Medicine, Guangzhou First People's Hospital of Guangzhou Medical University, Guangzhou, Guangdong, P. R. China.
authors: Meichun
authors: M
authors: Zhang
authors: Department of Respiratory Medicine, Guangzhou First People's Hospital of Guangzhou Medical University, Guangzhou, Guangdong, P. R. China.
authors: Jun
authors: J
authors: Zeng
authors: Department of Respiratory Medicine, Guangzhou First People's Hospital of Guangzhou Medical University, Guangzhou, Guangdong, P. R. China.
authors: Ziwen
authors: Z
authors: Zhao
authors: Department of Respiratory Medicine, Guangzhou First People's Hospital of Guangzhou Medical University, Guangzhou, Guangdong, P. R. China.
authors: Zhaohui
authors: Z
authors: Liu
mesh_terms: D020413:3' Untranslated Regions; D000072283:A549 Cells; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D018450:Disease Progression; D019008:Drug Resistance, Neoplasm; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D010750:Phosphoproteins / Q000235:genetics*; D011379:Prognosis; D011401:Promoter Regions, Genetic; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins
chemical_list: D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing; C554214:MIRN424 microrna, human; D035683:MicroRNAs; D010750:Phosphoproteins; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C088374:YAP1 protein, human
keywords: YAP1; chemoresistance; lung cancer; miR-424-3p; miR-424-5p