title: Real-time monitoring of glucose and phenols intestinal absorption through an integrated Caco-2TC7cells/biosensors telemetric device: Hypoglycemic effect of fruit phytochemicals.
abstract: An integrated device for real-time monitoring of glucose and phenols absorption, that consists of a sensors/biosensors system (SB) and a Caco-2TC7 human intestinal cell culture, is described in this study. The SB is composed of a glucose oxidase-based biosensor, a sentinel platinum sensor, a laccase/tyrosinase-based biosensor and a sentinel carbon sensor, all located in the basolateral compartment (BC) of a cell culture plate. Caco-2TC7 cells, differentiated on culture inserts, separated the apical compartment that simulates the intestinal lumen, from the BC which represented the bloodstream. The system recorded currents relative to glucose (1mM) absorption, obtaining bioavailability values (5.1%) comparable to HPLC analysis (4.8%). Phloridzin and phloretin, specific phenolic inhibitors of SGLT1 and GLUT2 glucose transporters, reduced the glucose transport of almost 10 times. They were minimally absorbed in the BC with a bioavailability of 0.13% and 0.49% respectively. The hypoglycemic potential of blueberry and pomegranate juices was also studied. In particular, the amount of glucose absorbed through the Caco-2TC7 monolayer was 8‰ for pomegranate and 1.7‰ for blueberry, demonstrating the potential hypoglycemic effect of the juices. Polyphenols absorption was also monitored by the SB and an increase was recorded during the first 50min in presence of both blueberry and pomegranate juices, then a constant decrease occurred. The proposed device has been developed as innovative tool for the dynamic monitoring of natural compounds effects on glucose absorption, in order to manage postprandial hyperglycemia.
authors: Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Traversa La Crucca, 3 Regione Baldinca, 07100 Li Punti, Sassari, Italy.
authors: Antonio
authors: A
authors: Barberis
authors: Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70125 Bari, Italy.
authors: Antonella
authors: A
authors: Garbetta
authors: Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70125 Bari, Italy.
authors: Angela
authors: A
authors: Cardinali
authors: Department of Clinical and Experimental Medicine, Section of Pharmacology, University of Sassari, V.le San Pietro 43/B, 07100 Sassari, Italy.
authors: Gianfranco
authors: G
authors: Bazzu
authors: Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70125 Bari, Italy.
authors: Isabella
authors: I
authors: D'Antuono
authors: Department of Clinical and Experimental Medicine, Section of Pharmacology, University of Sassari, V.le San Pietro 43/B, 07100 Sassari, Italy.
authors: Gaia
authors: G
authors: Rocchitta
authors: Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Traversa La Crucca, 3 Regione Baldinca, 07100 Li Punti, Sassari, Italy.
authors: Angela
authors: A
authors: Fadda
authors: Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70125 Bari, Italy.
authors: Vito
authors: V
authors: Linsalata
authors: Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Traversa La Crucca, 3 Regione Baldinca, 07100 Li Punti, Sassari, Italy.
authors: Guy
authors: G
authors: D'Hallewin
authors: Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Traversa La Crucca, 3 Regione Baldinca, 07100 Li Punti, Sassari, Italy; Department of Clinical and Experimental Medicine, Section of Pharmacology, University of Sassari, V.le San Pietro 43/B, 07100 Sassari, Italy. Electronic address: paserra@uniss.it.
authors: Pier Andrea
authors: PA
authors: Serra
authors: Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70125 Bari, Italy.
authors: Fiorenza
authors: F
authors: Minervini
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D029798:Blueberry Plants / Q000737:chemistry; D002460:Cell Line; D004867:Equipment Design; D000067030:Fruit and Vegetable Juices / Q000032:analysis; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D006943:Hyperglycemia / Q000188:drug therapy / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects; D029561:Lythraceae / Q000737:chemistry; D010636:Phenols / Q000378:metabolism*; D010693:Phloretin / Q000737:chemistry / Q000494:pharmacology*; D010695:Phlorhizin / Q000737:chemistry / Q000494:pharmacology*; D013686:Telemetry / Q000295:instrumentation
chemical_list: D007004:Hypoglycemic Agents; D010636:Phenols; D010695:Phlorhizin; D005947:Glucose; D010693:Phloretin
keywords: Caco-2TC7 cells; Enzyme biosensor; Glucose; Polyphenols; Postprandial hyperglycemia