title: Identification and screening of chemical constituents with hepatoprotective effects from three traditional Chinese medicines for treating jaundice.
abstract: The constituents with hepatoprotective activity were investigated in three traditional Chinese medicine formulae for treating jaundice, namely, Zhi-Zi-Da-Huang-Tang, Yin-Chen-Hao-Tang, and Da-Huang-Xiao-Shi-Tang. By using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and liquid chromatography coupled with ion trap mass spectrometry, 79 chemical constituents were identified unambiguously or tentatively in three formulae based on the accurate molecular weight, mass spectrometric fragmentation behavior, and comparison with reference standards. Then the hepatoprotective activities of 27 constituents were evaluated on tert-butylhydroperoxide-injured BRL-3A cells. The results indicated that 11 constituents, including protocatechic acid (19), epijasminoside A (56), rutin (71), tetrahydropalmatine (76), rhaponticin (80), 3,4-dicaffeoylquinic acid (82), 3,5-dicaffeoylquinic acid (85), diosmetin-7-O-glucoside (90), jatrorrhizine (93), berberine (100), and daidzein (101) exerted hepatoprotective activities. Interestingly, most of the crude drugs in three formulae contained hepatoprotective active constituents, and the combinations of constituents from different crude drugs exhibited greater effects. This result provided evidence to the traditional Chinese medicine theory of combining several drugs together to exert synergistic efficacy.
authors: Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P. R. China.
authors: Shufang
authors: S
authors: Wang
authors: Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P. R. China.
authors: Xiaodong
authors: X
authors: Li
authors: Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P. R. China.
authors: Yan
authors: Y
authors: Niu
authors: Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P. R. China.
authors: Yining
authors: Y
authors: Liu
authors: Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P. R. China.
authors: Yunxiang
authors: Y
authors: Zhu
authors: Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P. R. China.
authors: Xiaoyan
authors: X
authors: Lu
authors: Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P. R. China.
authors: Xiaohui
authors: X
authors: Fan
authors: School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, P. R. China.
authors: Xingxian
authors: X
authors: Zhang
authors: Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P. R. China. mysky@zju.edu.cn.
authors: Yi
authors: Y
authors: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D007565:Jaundice / Q000188:drug therapy*; D008099:Liver / Q000187:drug effects*; D008516:Medicine, Chinese Traditional; D020011:Protective Agents / Q000008:administration & dosage / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D020011:Protective Agents
keywords: Da-Huang-Xiao-Shi-Tang; Hepatoprotective activity; Mass spectrometry; Yin-Chen-Hao-Tang; Zhi-Zi-Da-Huang-Tang