title: Isolation of RNA from milk somatic cells as an alternative to biopsies of mammary tissue for nutrigenomic studies in dairy ewes.
abstract: Nutrigenomic studies of mammary lipogenesis in ruminants often rely on the use of mammary tissue (MT) collected either by biopsy or at slaughter. However, isolating RNA from milk would be a useful and cost-effective technique that may avoid distress to the animal and facilitate the collection of samples in time series experiments. This assay was therefore conducted to test the hypothesis that RNA extracted from milk somatic cells (MSC) in dairy sheep would be a feasible alternative to the performance of MT biopsies for nutrigenomic analyses. To meet this objective, 8 lactating Assaf ewes were divided in 2 groups and offered a total mixed ration without supplementation (control) or supplemented with 2.4% dry matter of fish oil, which was known not only to elicit milk fat depression but also to downregulate the expression of some candidate genes involved in mammary lipogenesis. Total RNA was extracted from MSC and biopsied MT to examine whether the potential changes in the abundance of transcripts was similarly detected with both RNA sources. Milk fatty acid profile was also analyzed by gas chromatography, and variations in mRNA abundance were determined by reverse transcription quantitative PCR. Values of RNA integrity number were always ≥7.7. The expected and designed decrease of milk fat concentration with fish oil (-29%), was associated with a lower transcript abundance of genes coding for enzymes involved in fatty acid activation (ACSS1), de novo synthesis (ACACA and FASN), uptake from plasma lipids (LPL), and esterification of fatty acids to glycerol (LPIN1), as well as of a transcription factor that may regulate their expression (INSIG1). Stable mRNA levels were showed in other candidate genes, such as FABP3, GPAT4, or SCD. Changes due to the dietary treatment were similarly detected with both RNA sources (MSC and MT biopsies), which supports the initial hypothesis and would validate the use of milk as an alternative RNA source for nutrigenomic analyses in dairy sheep.
authors: Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas s/n, 24346 Grulleros, León, Spain.
authors: P G
authors: PG
authors: Toral
authors: Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas s/n, 24346 Grulleros, León, Spain. Electronic address: g.hervas@csic.es.
authors: G
authors: G
authors: Hervás
authors: Departamento de Producción Animal, Facultad de Veterinaria, Universidad de León, 24071, León, Spain.
authors: A
authors: A
authors: Suárez-Vega
authors: Departamento de Producción Animal, Facultad de Veterinaria, Universidad de León, 24071, León, Spain.
authors: J J
authors: JJ
authors: Arranz
authors: Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas s/n, 24346 Grulleros, León, Spain.
authors: P
authors: P
authors: Frutos
mesh_terms: D000106:Acetate-CoA Ligase / Q000235:genetics / Q000378:metabolism; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001706:Biopsy; D003362:Cost-Benefit Analysis; D004032:Diet / Q000662:veterinary; D004041:Dietary Fats / Q000032:analysis; D019587:Dietary Supplements; D015536:Down-Regulation; D054890:Fatty Acid Synthase, Type I / Q000235:genetics / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage; D005990:Glycerol / Q000378:metabolism; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000235:genetics / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D050155:Lipogenesis; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008892:Milk / Q000737:chemistry*; D054647:Nutrigenomics / Q000379:methods*; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012756:Sheep
chemical_list: D004041:Dietary Fats; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005395:Fish Oils; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D012313:RNA; D005992:Glycerol-3-Phosphate O-Acyltransferase; D054890:Fatty Acid Synthase, Type I; D000106:Acetate-CoA Ligase; D005990:Glycerol
keywords: RNA source; fatty acid; fish oil; gene expression; sheep