title: Characterization and mesenteric lymph node cells-mediated immunomodulatory activity of litchi pulp polysaccharide fractions.
abstract: Three water-soluble hetero-polysaccharides, designated LP1-3, were isolated from litchi pulp. Their structures, solution properties and immunomodulatory activities were evaluated. LP1 contained (1→4,6)-β-d-Glc and (1→4)-α-l-Gal, while LP2 contained (1→3)-α-l-Ara and (l→2)-β-d-Gal, and LP3 contained α-l-Ara and (l→4)-β-Rha. Their molecular weights ranged from 105,880 to 986,470g/mol. LP1 had a spherical conformation with hyper-branched structure and LP2 was semi-flexible chain, while the polysaccharide chains of LP3 were cross linked to form network-like conformation in solution. In addition, all fractions strongly stimulated mesenteric lymph node cell proliferation, IFN-γ and IL-6 secretion in the dose range of 25-100μg/mL compared with untreated control group (p<0.05). LP1 exhibited the strongest stimulation of mesenteric lymph node cell proliferation and cytokine secretion, which may be attributed to its unique chemical structure and chain conformation. This is the first report on the solution properties and intestinal immunity activities of polysaccharides from litchi pulp.
authors: Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, PR China.
authors: Fei
authors: F
authors: Huang
authors: Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, PR China.
authors: Ruifen
authors: R
authors: Zhang
authors: Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, PR China.
authors: Yang
authors: Y
authors: Liu
authors: Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, PR China.
authors: Juan
authors: J
authors: Xiao
authors: Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, PR China.
authors: Dongxiao
authors: D
authors: Su
authors: College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China.
authors: Yang
authors: Y
authors: Yi
authors: College of Chemistry and Materials Science, Hubei Engineering University, Xiaogan 432000, PR China.
authors: Guangjin
authors: G
authors: Wang
authors: Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, PR China.
authors: Zhencheng
authors: Z
authors: Wei
authors: Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, PR China. Electronic address: mwzhh@vip.tom.com.
authors: Mingwei
authors: M
authors: Zhang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D005638:Fruit / Q000737:chemistry*; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007371:Interferon-gamma / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D032125:Litchi / Q000737:chemistry*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008643:Mesentery / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C000611649:IFNG protein, mouse; D007155:Immunologic Factors; D015850:Interleukin-6; D011134:Polysaccharides; C577686:interleukin-6, mouse; D007371:Interferon-gamma
keywords: Immunomodulatory activity; Litchi pulp; Mesenteric lymph node cells; Polysaccharide fractions; Structural features