title: Serpentinicella alkaliphila gen. nov., sp. nov., a novel alkaliphilic anaerobic bacterium isolated from the serpentinite-hosted Prony hydrothermal field, New Caledonia.
abstract: A novel anaerobic, alkaliphilic, Gram-stain-positive, spore-forming bacterium was isolated from a carbonaceous hydrothermal chimney in Prony Bay, New Caledonia. This bacterium, designated strain 3bT, grew at temperatures from 30 to 43 °C (optimum 37 °C) and at pH between 7.8 and 10.1 (optimum 9.5). Added NaCl was not required for growth (optimum 0-0.2 %, w/v), but was tolerated at up to 4 %. Yeast extract was required for growth. Strain 3bT utilized crotonate, lactate and pyruvate, but not sugars. Crotonate was dismutated to acetate and butyrate. Lactate was disproportionated to acetate and propionate. Pyruvate was degraded to acetate plus trace amounts of hydrogen. Growth on lactate was improved by the addition of fumarate, which was used as an electron acceptor and converted to succinate. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate, nitrite, FeCl3, Fe(III)-citrate, Fe(III)-EDTA, chromate, arsenate, selenate and DMSO were not used as terminal electron acceptors. The G+C content of the genomic DNA was 33.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the isolate is a member of the family Clostridiaceae, order Clostridiales within the phylum Firmicutes. Strain 3bT was most closely related to 'Alkaliphilus hydrothermalis' FatMR1T (92.2 % 16S rRNA gene sequence similarity), and was positioned approximately equidistantly between the genera Alkaliphilus, Anaerovirgula and Natronincola. On the basis of phylogenetic, genetic, chemotaxonomic and physiological properties, strain 3bT is proposed to represent a novel species of a new genus, for which the name Serpentinicella alkaliphila gen. nov., sp. nov. is proposed. The type strain of Serpentinicella alkaliphila is 3bT (=DSM 100013T=JCM 30645T).
authors: Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France.
authors: Nan
authors: N
authors: Mei
authors: Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France.
authors: Anne
authors: A
authors: Postec
authors: Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France.
authors: Gael
authors: G
authors: Erauso
authors: Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France.
authors: Manon
authors: M
authors: Joseph
authors: Centre IRD de Nouméa, 101 Promenade Roger Laroque, BP A5 - 98848 Nouméa cedex, New Caledonia.
authors: Bernard
authors: B
authors: Pelletier
authors: Centre IRD de Nouméa, 101 Promenade Roger Laroque, BP A5 - 98848 Nouméa cedex, New Caledonia.
authors: Claude
authors: C
authors: Payri
authors: Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France.
authors: Bernard
authors: B
authors: Ollivier
authors: Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France.
authors: Marianne
authors: M
authors: Quéméneur
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D061006:Bays; D000068016:Eubacteriales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D060086:Hydrothermal Vents / Q000382:microbiology*; D009510:New Caledonia; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S