title: MicroRNA-126 inhibits colon cancer cell proliferation and invasion by targeting the chemokine (C-X-C motif) receptor 4 and Ras homolog gene family, member A, signaling pathway.
abstract: MicroRNA-126 (miR-126) suppresses the migration, proliferation and invasion of colon cancer cells. However, the underlying mechanisms of miR-126 in colon cancer have not been fully elucidated. In this study, in vivo experiments revealed that miR-126 inhibits colon cancer growth and metastasis. Furthermore, miR-126 was down-regulated in human colon cancer tissue, and its expression was inversely correlated with TNM stage and metastasis of patients. Low level of miR-126 identified patients with poor prognosis. And we found that miR-126 expression was negatively correlated with the expression levels of chemokine (C-X-C motif) receptor 4 (CXCR4) and components of signaling pathway of Ras homolog gene family, member A (RhoA) in vitro and in vivo. Moreover, we verified that miR-126 negatively regulated CXCR4 and RhoA signaling in vitro. In addition, either in miR-126-overexpressing or in miR- 126-silenced colon cancer cells, the restoration of CXCR4 could significantly reverse the proliferation and invasion, as well as abolish the effects of miR-126 on RhoA signaling pathway. Collectively, these results demonstrated that miR-126 acts as a tumor suppressor by inactivating RhoA signaling via CXCR4 in colon cancer. And miR-126 may serve as a prognostic marker for monitoring and treating colon cancer.
authors: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha, Hunan, China.
authors: Wei
authors: W
authors: Yuan
authors: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.|Department of Gastroenterology, Yichang Central People's Hospital, Yichang, Hubei, China.
authors: Ye-Qing
authors: YQ
authors: Guo
authors: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha, Hunan, China.
authors: Xia-Yu
authors: XY
authors: Li
authors: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha, Hunan, China.
authors: Min-Zi
authors: MZ
authors: Deng
authors: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha, Hunan, China.
authors: Zhao-Hua
authors: ZH
authors: Shen
authors: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha, Hunan, China.
authors: Chi-Bin
authors: CB
authors: Bo
authors: Cancer Research Institute, Central South University, Key Laboratory of Carcinogenesis, Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Changsha, Hunan, China.
authors: Ya-Fei
authors: YF
authors: Dai
authors: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha, Hunan, China.
authors: Ming-Yu
authors: MY
authors: Huang
authors: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha, Hunan, China.
authors: Zhen-Yu
authors: ZY
authors: Yang
authors: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha, Hunan, China.
authors: Yong-Sheng
authors: YS
authors: Quan
authors: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha, Hunan, China.
authors: Li
authors: L
authors: Tian
authors: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha, Hunan, China.
authors: Xiaoyan
authors: X
authors: Wang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D008957:Models, Genetic; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D019718:Receptors, CXCR4 / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D020742:rhoA GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: C527276:CXCR4 protein, human; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D019718:Receptors, CXCR4; D020742:rhoA GTP-Binding Protein
keywords: CXCR4; RhoA; colon cancer; microRNA-126 (mir-126)