title: Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq.
abstract: The white-rot fungus Phlebia sp. MG-60 shows valuable properties such as high ethanol yield from several lignocellulosic materials, although white-rot fungi commonly degrade woody components to CO2 and H2O. In order to identify genes involved in ethanol production by Phlebia sp. MG-60, we compared genes differentially expressed by the ethanol producing fungus Phlebia sp. MG-60 and the model white-rot fungus Phanerochaete chrysosporium under ethanol fermenting and non-fermenting conditions using next-generation sequencing technologies.
  
  
  
  mRNAs from mycelia of Phlebia sp. MG-60 and P. chrysosporium under fermenting and non-fermenting conditions were sequenced using the MiSeq system. To detect differentially expressed genes, expression levels were measured in fragments per kilobase of exon per million mapped reads (FPKM). Differentially expressed genes were annotated using BLAST searches, Gene Ontology classifications, and KEGG pathway analysis. Functional analyses of differentially expressed genes revealed that genes involved in glucose uptake, glycolysis, and ethanol synthesis were widely upregulated in Phlebia sp. MG-60 under fermenting conditions.
  
  
  
  In this study, we provided novel transcriptomic information on Phlebia sp. MG-60, and these RNA-seq data were useful in targeting genes involved in ethanol production for future genetic engineering.
authors: Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
authors: Jianqiao
authors: J
authors: Wang
authors: Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, Utsunomiya, 321-8505, Japan.
authors: Tomohiro
authors: T
authors: Suzuki
authors: Institute for Genetic Research and Biotechnology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.|Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
authors: Hideo
authors: H
authors: Dohra
authors: Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
authors: Shoko
authors: S
authors: Takigami
authors: Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
authors: Hiroko
authors: H
authors: Kako
authors: Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
authors: Ayumi
authors: A
authors: Soga
authors: Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-kibanadai-nishi, Miyazaki, 889-2192, Japan.
authors: Ichiro
authors: I
authors: Kamei
authors: Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
authors: Toshio
authors: T
authors: Mori
authors: Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.|Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.|Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
authors: Hirokazu
authors: H
authors: Kawagishi
authors: Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. hirai.hirofumi@shizuoka.ac.jp.|Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. hirai.hirofumi@shizuoka.ac.jp.
authors: Hirofumi
authors: H
authors: Hirai
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D063990:Gene Ontology; D005947:Glucose / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D020075:Phanerochaete / Q000235:genetics / Q000378:metabolism; D020072:Polyporales / Q000235:genetics* / Q000378:metabolism; D014934:Wood / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D000431:Ethanol; D005947:Glucose
keywords: Ethanol fermentation mechanism; Phlebia sp. MG-60; RNA-seq; White-rot fungi