title: Detection and Identification of Ligands for Mammalian RPTP Extracellular Domains.
abstract: Receptor protein tyrosine phosphatases (RPTPs) form a group of over 20 enzymes in vertebrates, each with unique ectodomains subject to potential extracellular interactions with ligands. It has recently become clear that a remarkably diverse range of ligands exist, including homophilic binders, adhesion molecules, neurotrophin receptors, and proteoglycans. Individual RPTPs can bind several ligands, and vice versa, suggesting that complex cell signaling networks exist. The identification of RPTP ligands and where they are located in tissues remains a challenge for a large number of these enzymes. Here we describe some powerful methods that have proved successful for several research groups, leading to our improved understanding of RPTP-ligand interactions and functional regulation.
authors: UCL Institute of Child Health, University College London, 30 Guilford Street, London, WC1N 1EH, UK. a.stoker@ucl.ac.uk.
authors: Andrew William
authors: AW
authors: Stoker
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D000818:Animals; D019556:COS Cells; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D002846:Chromatography, Affinity / Q000379:methods*; D018920:Coculture Techniques / Q000379:methods; D058851:GPI-Linked Proteins / Q000032:analysis / Q000378:metabolism; D057809:HEK293 Cells; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism; D008024:Ligands; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding; D000072417:Protein Domains; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D051381:Rats; D054557:Receptor-Like Protein Tyrosine Phosphatases / Q000032:analysis / Q000378:metabolism*
chemical_list: D058851:GPI-Linked Proteins; D007527:Isoenzymes; D008024:Ligands; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental; D054557:Receptor-Like Protein Tyrosine Phosphatases
keywords: Affinity chromatography; Affinity probe; Hippocampal neuron; Ligand; Neurite; Placental alkaline phosphatase; RAP assay; RPTP; Receptor tyrosine phosphatase; Synapse