title: Effects of low-level laser therapy on autogenous bone graft stabilized with a new heterologous fibrin sealant.
abstract: Autogenous bone grafts are used to repair bone defects, and the stabilization is needed for bone regeneration. Laser photobiomodulation is a modality of treatment in clinical practice for tissue regeneration, and it has therapeutic effects as an anti-inflammatory, analgesic and modulating cellular activity. The aim of the present study was to evaluate the effects of low-level laser therapy (LLLT) on an autogenous bone graft integration process stabilized with a new heterologous fibrin sealant. Forty rats were divided into two groups: Autogenous Fibrin Graft (AFG, n=20), in which a 5mm dome osteotomy was conducted in the right parietal bone and the graft was adhered to the left side using fibrin sealant; and Autogenous Fibrin Graft Laser (AFGL, n=20), which was subjected to the same procedures as AFG with the addition of LLLT. The treatment was performed immediately following surgery and then three times a week until euthanasia, using an 830nm laser (30mW, 6J/cm(2), 0.116cm(2), 258.6mW/cm(2), 2.9J). Five animals from each group were euthanized at 10, 20, 30 and 40days postoperative, and the samples were submitted to histomorphological and histomorphometric analysis. Partial bone regeneration occurred, with new bone tissue integrating the graft to the recipient bed and small areas of connective tissue. Comparative analysis of the groups at the same intervals revealed minor interfaces in group AFGL, with statistically significant differences (p<0.05) at all of the analyzed intervals (10days p=0.0087, 20days p=0.0012, 30days p<0.0001, 40days p=0.0142). In conclusion, low-level laser therapy stimulated bone regeneration and accelerated the process of integration of autogenous bone grafts.
authors: Faculty of Medicine, University of Marília (UNIMAR), Marília, SP, Brazil; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors: Jéssica Barbosa
authors: JB
authors: de Oliveira Gonçalves
authors: Faculty of Medicine, University of Marília (UNIMAR), Marília, SP, Brazil; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors: Daniela Vieira
authors: DV
authors: Buchaim
authors: University of the Sacred Heart, Bauru, SP, Brazil.
authors: Cleuber Rodrigo
authors: CR
authors: de Souza Bueno
authors: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors: Karina Torres
authors: KT
authors: Pomini
authors: Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP, Brazil.
authors: Benedito
authors: B
authors: Barraviera
authors: Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP, Brazil.
authors: Rui Seabra Ferreira
authors: RSF
authors: Júnior
authors: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors: Jesus Carlos
authors: JC
authors: Andreo
authors: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors: Antonio
authors: A
authors: de Castro Rodrigues
authors: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors: Tania Mary
authors: TM
authors: Cestari
authors: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: rogerio@fob.usp.br.
authors: Rogério Leone
authors: RL
authors: Buchaim
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000628:therapy*; D001861:Bone Regeneration; D016025:Bone Transplantation; D001842:Bone and Bones / Q000473:pathology; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015718:Fibrin Tissue Adhesive
keywords: Bone graft; Bone regeneration; Fibrin sealant; Laser therapy; Osseous defects