title: Electrochemically reduced graphene and iridium oxide nanoparticles for inhibition-based angiotensin-converting enzyme inhibitor detection.
abstract: In this work, a novel biosensor based on electrochemically reduced graphene oxide and iridium oxide nanoparticles for the detection of angiotensin-converting enzyme inhibitor drug, captopril, is presented. For the preparation of the biosensor, tyrosinase is immobilized onto screen printed electrode by using 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-Hydroxysuccinimide coupling reagents, in electrochemically reduced graphene oxide and iridium oxide nanoparticles matrix. Biosensor response is characterized towards catechol, in terms of graphene oxide concentration, number of cycles to reduce graphene oxide, volume of iridium oxide nanoparticles and tyrosinase solution. The designed biosensor is used to inhibit tyrosinase activity by Captopril, which is generally used to treat congestive heart failure. It is an angiotensin-converting enzyme inhibitor that operates via chelating copper at the active site of tyrosinase and thioquinone formation. The captopril detections using both inhibition ways are very sensitive with low limits of detection: 0.019µM and 0.008µM for chelating copper at the active site of tyrosinase and thioquinone formation, respectively. The proposed methods have been successfully applied in captopril determination in spiked human serum and pharmaceutical dosage forms with acceptable recovery values.
authors: Nanobioelectronics and Biosensors Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, Barcelona 08193, Spain; Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Tandogan, Ankara 06100, Turkey.
authors: Sevinc
authors: S
authors: Kurbanoglu
authors: Nanobioelectronics and Biosensors Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, Barcelona 08193, Spain.
authors: Lourdes
authors: L
authors: Rivas
authors: Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Tandogan, Ankara 06100, Turkey.
authors: Sibel A
authors: SA
authors: Ozkan
authors: Nanobioelectronics and Biosensors Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, Barcelona 08193, Spain; ICREA, Pg. Lluís Companys, 23, Barcelona 08010, Spain. Electronic address: arben.merkoci@icn2.cat.
authors: Arben
authors: A
authors: Merkoçi
mesh_terms: D000363:Agaricales / Q000201:enzymology; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis* / Q000097:blood* / Q000494:pharmacology; D015374:Biosensing Techniques / Q000379:methods*; D002216:Captopril / Q000032:analysis* / Q000097:blood* / Q000494:pharmacology; D055664:Electrochemical Techniques / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007495:Iridium / Q000737:chemistry*; D057230:Limit of Detection; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010084:Oxidation-Reduction
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D004800:Enzymes, Immobilized; C044458:iridium oxide; D007495:Iridium; D006108:Graphite; D002216:Captopril; D014442:Monophenol Monooxygenase
keywords: Angiotensin-converting enzyme inhibitor; Captopril detection; Electrochemically reduced graphene oxide; Enzyme biosensors; Enzyme inhibition; Iridium oxide nanoparticles