title: Chronic oral administration of pine bark extract (flavangenol) attenuates brain and liver mRNA expressions of HSPs in heat-exposed chicks.
abstract: Exposure to a high ambient temperature (HT) can cause heat stress, which has a huge negative impact on physiological functions. Cellular heat-shock response is activated upon exposure to HT for cellular maintenance and adaptation. In addition, antioxidants are used to support physiological functions under HT in a variety of organisms. Flavangenol, an extract of pine bark, is one of the most potent antioxidants with its complex mixture of polyphenols. In the current study, chronic (a single daily oral administration for 14 days) or acute (a single oral administration) oral administration of flavangenol was performed on chicks. Then the chicks were exposed to an acute HT (40±1°C for 3h) to examine the effect of flavangenol on the mRNA expression of heat-shock protein (HSP) in the brain and liver. Rectal temperature, plasma aspartate aminotransferase (AAT), a marker of liver damage, and plasma corticosterone as well as metabolites were also determined. HSP-70 and -90 mRNA expression, rectal temperature, plasma AAT and corticosterone were increased by HT. Interestingly, the chronic, but not the acute, administration of flavangenol caused a declining in the diencephalic mRNA expression of HSP-70 and -90 and plasma AAT in HT-exposed chicks. Moreover, the hepatic mRNA expression of HSP-90 was also significantly decreased by chronic oral administration of flavangenol in HT chicks. These results indicate that chronic, but not acute, oral administration of flavangenol attenuates HSP mRNA expression in the central and peripheral tissues due to its possible role in improving cellular protective functions during heat stress. The flavangenol-dependent decline in plasma AAT further suggests that liver damage induced by heat stress was minimized by flavangenol.
authors: Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Hui
authors: H
authors: Yang
authors: Division for Experimental Natural Science, Faculty of Arts and Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, Japan. Electronic address: vc-sur@artsci.kyushu-u.ac.jp.
authors: Vishwajit S
authors: VS
authors: Chowdhury
authors: Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Mohammad A
authors: MA
authors: Bahry
authors: Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Phuong V
authors: PV
authors: Tran
authors: Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Phong H
authors: PH
authors: Do
authors: Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Guofeng
authors: G
authors: Han
authors: Division of Endocrinology, Department of Medicine, Boston Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA.
authors: Rong
authors: R
authors: Zhang
authors: Research and Development Division, Toyo Shinyaku Co. Ltd., Saga 841-0005, Japan.
authors: Hideki
authors: H
authors: Tagashira
authors: Research and Development Division, Toyo Shinyaku Co. Ltd., Saga 841-0005, Japan.
authors: Masahito
authors: M
authors: Tsubata
authors: Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors: Mitsuhiro
authors: M
authors: Furuse
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000097:blood; D044946:Biflavonoids / Q000008:administration & dosage / Q000627:therapeutic use*; D002645:Chickens / Q000097:blood / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D018882:Heat Stress Disorders / Q000097:blood / Q000378:metabolism / Q000517:prevention & control / Q000662:veterinary; D006360:Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response / Q000187:drug effects*; D008297:Male; D028223:Pinus / Q000737:chemistry; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use; D011201:Poultry Diseases / Q000097:blood / Q000378:metabolism / Q000517:prevention & control; D044945:Proanthocyanidins / Q000008:administration & dosage / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D006360:Heat-Shock Proteins; D010936:Plant Extracts; D044945:Proanthocyanidins; D012333:RNA, Messenger; C504746:flavangenol; D001219:Aspartate Aminotransferases
keywords: Chicks; Flavangenol; Heat stress; Heat-shock protein gene