title: Identification and comparative oridonin metabolism in different species liver microsomes by using UPLC-Triple-TOF-MS/MS and PCA.
abstract: Oridonin (ORI) is an active natural ent-kaurene diterpenoid ingredient with notable anti-cancer and anti-inflammation activities. Currently, a strategy was developed to identify metabolites and to assess the metabolic profiles of ORI in vitro using ultra-high-performance liquid chromatography-Triple/time-of-flight mass spectrometry (UPLC-Triple-TOF-MS/MS). Meanwhile, the metabolism differences of ORI in the liver microsomes of four different species were investigated using a principal component analysis (PCA) based on the metabolite absolute peak area values as the variables. Based on the proposed methods, 27 metabolites were structurally characterized. The results indicate that ORI is universally metabolized in vitro, and the metabolic pathway mainly includes dehydration, hydroxylation, di-hydroxylation, hydrogenation, decarboxylation, and ketone formation. Overall, there are obvious inter-species differences in types and amounts of ORI metabolites in the four species. These results will provide basic data for future pharmacological and toxicological studies of ORI and for other ent-kauranes diterpenoids. Meanwhile, studying the ORI metabolic differences helps to select the proper animal model for further pharmacology and toxicological assessment.
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China.
authors: Yinghua
authors: Y
authors: Ma
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China.
authors: Weiwei
authors: W
authors: Xie
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China.
authors: Tingting
authors: T
authors: Tian
authors: The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, PR China.
authors: Yiran
authors: Y
authors: Jin
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China.
authors: Huijun
authors: H
authors: Xu
authors: Applied Biosystems Trading Co., Ltd., Beijing Branch Office, Beijing 100027, PR China.
authors: Kerong
authors: K
authors: Zhang
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China. Electronic address: yingfengdu@hotmail.com.
authors: Yingfeng
authors: Y
authors: Du
mesh_terms: D000818:Animals; D045786:Diterpenes, Kaurane / Q000493:pharmacokinetics*; D006801:Humans; D008862:Microsomes, Liver / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D045786:Diterpenes, Kaurane; C011959:oridonin
keywords: In vitro; Liver microsomes; Metabolites; Oridonin; UPLC-Triple-TOF-MS/MS