title: Systematic identification of immunodominant CD4+ T cell responses to HpaA in Helicobacter pylori infected individuals.
abstract: In mice, antigen-specific CD4+ T cell response is indispensible for the protective immunity against Helicobacter pylori (H. pylori). It has been demonstrated that neuraminyllactose-binding hemagglutinin (HpaA) immunization protected mice from H. pylori infection in a CD4+ T cell dependent manner. However, much remains unclear concerning the human CD4+ T cell responses to HpaA. We conducted a systematic study here to explore the immunodominant, HpaA-specific CD4+ T cell responses in H. pylori infected individuals. We found that HpaA-specific CD4+ T cell responses varied remarkably in their magnitude and had broad epitope-specificity. Importantly, the main responses focused on two regions: HpaA76-105 and HpaA130-159. The HLA-DRB1*0901 restricted HpaA142-159 specific CD4+ T cell response was the most immunodominant response at a population level. The immunodominant epitope HpaA142-159 was naturally presented and highly conserved. We also demonstrated that it was not the broad peptide specificity, but the strength of HpaA specific CD4+ T cell responses associated with gastric diseases potentially caused by H. pylori infection. Such investigation will aid development of novel vaccines against H. pylori infection.
authors: Department of Gastroenterology, The Second Affiliated Hospital, Third Military Medical University, Chongqing, PR China.|Department of Intensive Care Unit, Chengdu Military General Hospital, Chengdu, PR China.
authors: Jian
authors: J
authors: Hu
authors: National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University, Chongqing, PR China.|Department of Blood Transfusion, The Second Affiliated Hospital, Third Military Medical University, Chongqing, PR China.
authors: Li
authors: L
authors: Chen
authors: Department of Gastroenterology, The Second Affiliated Hospital, Third Military Medical University, Chongqing, PR China.|Department of Hematology, The Second Affiliated Hospital, Third Military Medical University, Chongqing, PR China.
authors: Wuchen
authors: W
authors: Yang
authors: National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University, Chongqing, PR China.
authors: Bin
authors: B
authors: Li
authors: National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University, Chongqing, PR China.
authors: Heqiang
authors: H
authors: Sun
authors: Department of Gastroenterology, The Second Affiliated Hospital, Third Military Medical University, Chongqing, PR China.
authors: Shanshan
authors: S
authors: Wei
authors: Department of Gastroenterology, The Second Affiliated Hospital, Third Military Medical University, Chongqing, PR China.
authors: Yafei
authors: Y
authors: He
authors: National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University, Chongqing, PR China.
authors: Zhuo
authors: Z
authors: Zhao
authors: Department of Gastroenterology, The Second Affiliated Hospital, Third Military Medical University, Chongqing, PR China.
authors: Shiming
authors: S
authors: Yang
authors: National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University, Chongqing, PR China.
authors: Quanming
authors: Q
authors: Zou
authors: T Cell Laboratory, La Trobe Institute for Molecular Science, School of Molecular Science, La Trobe University, Bundoora, Victoria, Australia.
authors: Weisan
authors: W
authors: Chen
authors: Department of Gastroenterology, The Second Affiliated Hospital, Third Military Medical University, Chongqing, PR China.
authors: Hong
authors: H
authors: Guo
authors: National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University, Chongqing, PR China.
authors: Chao
authors: C
authors: Wu
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D059811:HLA-DRB1 Chains / Q000032:analysis; D016481:Helicobacter Infections / Q000150:complications / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D013272:Stomach Diseases / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D059811:HLA-DRB1 Chains; C095977:adhesin A, Helicobacter
keywords: HLA-DRB1*0901; Helicobacter pylori; HpaA; Immune response; Immunology and Microbiology Section; immunodominant epitope; Immunity