title: Inhibition of Endoplasmic Reticulum Stress is Involved in the Neuroprotective Effect of bFGF in the 6-OHDA-Induced Parkinson's Disease Model.
abstract: Parkinson's disease (PD) is a progressive neurodegenerative disorder with complicated pathophysiologic mechanisms. Endoplasmic reticulum (ER) stress appears to play a critical role in the progression of PD. We demonstrated that basic fibroblast growth factor (bFGF), as a neurotropic factor, inhibited ER stress-induced neuronal cell apoptosis and that 6-hydroxydopamine (6-OHDA)-induced ER stress was involved in the progression of PD in rats. bFGF administration improved motor function recovery, increased tyrosine hydroxylase (TH)-positive neuron survival, and upregulated the levels of neurotransmitters in PD rats. The 6-OHDA-induced ER stress response proteins were inhibited by bFGF treatment. Meanwhile, bFGF also increased expression of TH. The administration of bFGF activated the downstream signals PI3K/Akt and Erk1/2 in vivo and in vitro. Inhibition of the PI3K/Akt and Erk1/2 pathways by specific inhibitors partially reduced the protective effect of bFGF. This study provides new insight towards bFGF translational drug development for PD involving the regulation of ER stress.
authors: 1School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors: Pingtao
authors: P
authors: Cai
authors: 1School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors: Jingjing
authors: J
authors: Ye
authors: 1School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors: Jingjing
authors: J
authors: Zhu
authors: 1School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors: Dan
authors: D
authors: Liu
authors: 2Emergency Department, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors: Daqing
authors: D
authors: Chen
authors: 3Department of Neurosurgery, Cixi People's Hospital, Wenzhou Medical University, Ningbo, Zhejiang, 315300, China.
authors: Xiaojie
authors: X
authors: Wei
authors: 4Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA.
authors: Noah R
authors: NR
authors: Johnson
authors: 1School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors: Zhouguang
authors: Z
authors: Wang
authors: 1School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors: Hongyu
authors: H
authors: Zhang
authors: 4Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA.
authors: Guodong
authors: G
authors: Cao
authors: 1School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors: Jian
authors: J
authors: Xiao
authors: 5Department of Anesthesia, the First Affiliated Hospital, Gannan Medical College, Ganzhou, 341000, China.
authors: Junming
authors: J
authors: Ye
authors: 1School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors: Li
authors: L
authors: Lin
keywords: 6-OHDA; ER stress; Parkinson's disease; bFGF