title: Fluorescent molecularly imprinted nanogels for the detection of anticancer drugs in human plasma.
abstract: Several fluorescent molecularly imprinted nanogels for the detection of the anticancer drug sunitinib were synthesized and characterized. A selection of functional monomers based on different aminoacids and coumarin allowed isolation of polymers with very good rebinding properties and sensitivities. The direct detection of sunitinib in human plasma was successfully demonstrated by fluorescence quenching of the coumarin-based nanogels. The plasma sample simply diluted in DMSO allowed the recovery of various amounts of sunitib, as determined by an averaged calibration curve. The LOD was 400nM, with within-run variability <9%, day to day variability <5%, and good accuracy in the recovery of sunitinib from spiked samples.
authors: Dipartimento di Scienze Chimiche e Farmaceutiche, Università degli Studi di Trieste, Italy; Graduate School of Nanotechnology, Università degli Studi di Trieste, Italy. Electronic address: elena.pellizzoni@phd.units.it.
authors: Elena
authors: E
authors: Pellizzoni
authors: Dipartimento di Scienze Chimiche e Farmaceutiche, Università degli Studi di Trieste, Italy. Electronic address: martina.tommasini@phd.units.it.
authors: Martina
authors: M
authors: Tommasini
authors: CRO National Cancer Institute Experimental and Clinical Pharmacology Division, Department of Translational Research, Aviano, Italy. Electronic address: emarangon@cro.it.
authors: Elena
authors: E
authors: Marangon
authors: CRO National Cancer Institute Experimental and Clinical Pharmacology Division, Department of Translational Research, Aviano, Italy. Electronic address: frizzolio@cro.it.
authors: Flavio
authors: F
authors: Rizzolio
authors: Department of Chemistry and Biochemistry, SBCS, Queen Mary University of London, Mile End Road, London E1 4NS, UK. Electronic address: g.saito@qmul.ac.uk.
authors: Gabriele
authors: G
authors: Saito
authors: Dipartimento di Scienze Chimiche e Farmaceutiche, Università degli Studi di Trieste, Italy. Electronic address: benedett@units.it.
authors: Fabio
authors: F
authors: Benedetti
authors: CRO National Cancer Institute Experimental and Clinical Pharmacology Division, Department of Translational Research, Aviano, Italy. Electronic address: gtoffoli@cro.it.
authors: Giuseppe
authors: G
authors: Toffoli
authors: Department of Chemistry and Biochemistry, SBCS, Queen Mary University of London, Mile End Road, London E1 4NS, UK. Electronic address: m.resmini@qmul.ac.uk.
authors: Marina
authors: M
authors: Resmini
authors: Dipartimento di Scienze Chimiche e Farmaceutiche, Università degli Studi di Trieste, Italy. Electronic address: fberti@units.it.
authors: Federico
authors: F
authors: Berti
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood / Q000737:chemistry; D001774:Blood Chemical Analysis / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods*; D007211:Indoles / Q000097:blood* / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D011758:Pyrroles / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D000077210:Sunitinib
chemical_list: D000970:Antineoplastic Agents; D007211:Indoles; D000080385:Nanogels; D011758:Pyrroles; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D000077210:Sunitinib
keywords: Anticancer drugs; Fluorimetry; Human plasma; Imprinted polymers; Therapeutic drug monitoring