title: Rapid Identification of the Receptor-Binding Specificity of Influenza A Viruses by Fluorogenic Glycofoldamers.
abstract: The re-emergence of influenza raises a global concern that viral pandemics can unpredictably occur. However, effective approaches that can probe the infection risk of influenza viruses for humans are rare. In this work, we develop a glycofoldamer that can rapidly identify the glycan-receptor specificity of influenza viruses in a high-throughput manner. The coupling of glycan receptors that can be recognized by hemagglutinin (a surface protein on the virion capsid of influenza) to a fluorogenic-dye foldamer produces the glycofoldamers with minimal fluorescence in aqueous solution. After interaction with human-infecting virus strains for only five minutes, the fluorescence intensity of the glycofoldamer is remarkably enhanced with a blue-shifted emission peak. The probes have also proven effective for the rapid identification of 1) the human- or bird-infecting properties of influenza viruses in a high-throughput manner and 2) the receptor-specificity switch of a virus strain by mutations.
authors: Key Laboratory for Advanced Materials & Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, PR China. xphe@ecust.edu.cn.
authors: Xiao-Peng
authors: XP
authors: He
authors: Key Laboratory for Advanced Materials & Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, PR China.
authors: Ya-Li
authors: YL
authors: Zeng
authors: Vaccine Research Center, Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, 200031, China.
authors: Xin-Ying
authors: XY
authors: Tang
authors: National Center for Protein Science Shanghai, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
authors: Na
authors: N
authors: Li
authors: Vaccine Research Center, Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, 200031, China. dmzhou@sibs.ac.cn.
authors: Dong-Ming
authors: DM
authors: Zhou
authors: Key Laboratory for Advanced Materials & Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, PR China.
authors: Guo-Rong
authors: GR
authors: Chen
authors: Key Laboratory for Advanced Materials & Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, PR China. tianhe@ecust.edu.cn.
authors: He
authors: H
authors: Tian
mesh_terms: D000818:Animals; D001665:Binding Sites; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D011134:Polysaccharides
keywords: fluorescence spectroscopy; foldamers; glycans; high-throughput screening; influenza