title: Identification and discrimination of three common Aloe species by high performance liquid chromatography-tandem mass spectrometry coupled with multivariate analysis.
abstract: Aloe arborescens, Aloe barbadensis and Aloe ferox are the most widely cultivated and used among 500 aloe species due to their potent bioactivity. However, the difference of aloe species is neglected and labeled only one name Aloe in the market without specifying aloe species discrimination in general. Furthermore, differences in bioactivity and side effects from different aloe species have not been well investigated. This study develops an effective method for simultaneous qualitative and quantitative determination of three common aloe species using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and high performance liquid chromatography-diode array detection (HPLC-DAD). The extraction conditions were optimized by response surface methodology (RSM) based on methanol concentration, extraction time and solvent-to-material ratio. A partial least squares-discriminant analysis (PLS-DA) was used to identify the three aloe species. The developed HPLC-MS/MS method coupled with multivariate analysis can be applied to discriminate three aloe species successfully.
authors: College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea.
authors: Yan
authors: Y
authors: Zhao
authors: College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea.
authors: Ya Nan
authors: YN
authors: Sun
authors: College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea.
authors: Min Jung
authors: MJ
authors: Lee
authors: College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea.
authors: Young Ho
authors: YH
authors: Kim
authors: College of Pharmacy, Chosun National University, Gwangju, Republic of Korea.
authors: Wonjae
authors: W
authors: Lee
authors: College of Pharmacy, Kangwon National University, Chuncheon, Republic of Korea.
authors: Kyeong Ho
authors: KH
authors: Kim
authors: College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea. Electronic address: devilkkt@nate.com.
authors: Kyung Tae
authors: KT
authors: Kim
authors: College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea. Electronic address: kangjss@cnu.ac.kr.
authors: Jong Seong
authors: JS
authors: Kang
mesh_terms: D000504:Aloe / Q000145:classification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015999:Multivariate Analysis; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods*
keywords: Aloe; LC-ESI–MS/MS; Multivariate analysis; Response surface methodology