title: Gelatin Methacrylate Hydrogels as Biomimetic Three-Dimensional Matrixes for Modeling Breast Cancer Invasion and Chemoresponse in Vitro.
abstract: Recent studies have shown that three-dimensional (3D) culture environments allow the study of cellular responses in a setting that more closely resembles the in vivo milieu. In this context, hydrogels have become popular scaffold options for the 3D cell culture. Because the mechanical and biochemical properties of culture matrixes influence crucial cell behavior, selecting a suitable matrix for replicating in vivo cellular phenotype in vitro is essential for understanding disease progression. Gelatin methacrylate (GelMA) hydrogels have been the focus of much attention because of their inherent bioactivity, favorable hydration and diffusion properties, and ease-of-tailoring of their physicochemical characteristics. Therefore, in this study we examined the efficacy of GelMA hydrogels as a suitable platform to model specific attributes of breast cancer. We observed increased invasiveness in vitro and increased tumorigenic ability in vivo in breast cancer cells cultured on GelMA hydrogels. Further, cells cultured on GelMA matrixes were more resistant to paclitaxel treatment, as shown by the results of cell-cycle analysis and gene expression. This study, therefore, validates GelMA hydrogels as inexpensive, cell-responsive 3D platforms for modeling key characteristics associated with breast cancer metastasis, in vitro.
authors: Anti-Cancer Technologies Program, Mazumdar Shaw Center for Translational Research , Narayana Hrudayalaya Health City, Hosur Road, Bangalore 560 099, India.
authors: Anuradha D
authors: AD
authors: Arya
authors: Anti-Cancer Technologies Program, Mazumdar Shaw Center for Translational Research , Narayana Hrudayalaya Health City, Hosur Road, Bangalore 560 099, India.
authors: Pavan M
authors: PM
authors: Hallur
authors: Department of Mechanical Engineering, Indian Institute of Science , Bangalore 560 012, India.
authors: Abhijith G
authors: AG
authors: Karkisaval
authors: Anti-Cancer Technologies Program, Mazumdar Shaw Center for Translational Research , Narayana Hrudayalaya Health City, Hosur Road, Bangalore 560 099, India.
authors: Aditi
authors: A
authors: Gudipati
authors: In Vivo Pharmacology-Oncology, Syngene International Ltd. , Plot Nos. 2 & 3, Bommasandra IV Phase, Jigani Link Road, Bangalore 560 099, India.
authors: Satheesh
authors: S
authors: Rajendiran
authors: In Vivo Pharmacology-Oncology, Syngene International Ltd. , Plot Nos. 2 & 3, Bommasandra IV Phase, Jigani Link Road, Bangalore 560 099, India.
authors: Vaibhav
authors: V
authors: Dhavale
authors: In Vivo Pharmacology-Oncology, Syngene International Ltd. , Plot Nos. 2 & 3, Bommasandra IV Phase, Jigani Link Road, Bangalore 560 099, India.
authors: Balaji
authors: B
authors: Ramachandran
authors: In Vivo Pharmacology-Oncology, Syngene International Ltd. , Plot Nos. 2 & 3, Bommasandra IV Phase, Jigani Link Road, Bangalore 560 099, India.
authors: Aravindakshan
authors: A
authors: Jayaprakash
authors: Department of Mechanical Engineering, Indian Institute of Science , Bangalore 560 012, India.
authors: Namrata
authors: N
authors: Gundiah
authors: Anti-Cancer Technologies Program, Mazumdar Shaw Center for Translational Research , Narayana Hrudayalaya Health City, Hosur Road, Bangalore 560 099, India.
authors: Aditya
authors: A
authors: Chaubey
mesh_terms: D032701:Biomimetics; D001943:Breast Neoplasms; D005780:Gelatin; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008689:Methacrylates; D009361:Neoplasm Invasiveness
chemical_list: D020100:Hydrogels; D008689:Methacrylates; D005780:Gelatin
keywords: 3D culture; breast cancer; chemoresponse; gelatin methacrylate; hydrogels; invasiveness