title: Gold-Induced Fibril Growth: The Mechanism of Surface-Facilitated Amyloid Aggregation.
abstract: The question of how amyloid fibril formation is influenced by surfaces is crucial for a detailed understanding of the process in vivo. We applied a combination of kinetic experiments and molecular dynamics simulations to elucidate how (model) surfaces influence fibril formation of the amyloid-forming sequences of prion protein SUP35 and human islet amyloid polypeptide. The kinetic data suggest that structural reorganization of the initial peptide corona around colloidal gold nanoparticles is the rate-limiting step. The molecular dynamics simulations reveal that partial physisorption to the surface results in the formation of aligned monolayers, which stimulate the formation of parallel, critical oligomers. The general mechanism implies that the competition between the underlying peptide-peptide and peptide-surface interactions must strike a balance to accelerate fibril formation.
authors: Chemistry Department, Leibniz Institute of Surface Modification (IOM) Leipzig e.V., Permoserstrasse 15, 04318, Leipzig, Germany.|Faculty of Chemistry and Mineralogy, University of Leipzig, Linnéstrasse 3, 04193, Leipzig, Germany.
authors: Anika
authors: A
authors: Gladytz
authors: Chemistry Department, Leibniz Institute of Surface Modification (IOM) Leipzig e.V., Permoserstrasse 15, 04318, Leipzig, Germany. bernd.abel@iom-leipzig.de.|Faculty of Chemistry and Mineralogy, University of Leipzig, Linnéstrasse 3, 04193, Leipzig, Germany. bernd.abel@iom-leipzig.de.
authors: Bernd
authors: B
authors: Abel
authors: Chemistry Department, Leibniz Institute of Surface Modification (IOM) Leipzig e.V., Permoserstrasse 15, 04318, Leipzig, Germany. hrissel@gwdg.de.|Department of Theoretical Physics, Georg-August-University, Friedrich-Hund-Platz 1, 37077, Göttingen, Germany. hrissel@gwdg.de.
authors: Herre Jelger
authors: HJ
authors: Risselada
mesh_terms: D006046:Gold / Q000737:chemistry*; D006801:Humans; D058228:Islet Amyloid Polypeptide / Q000737:chemistry*; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D000072002:Prion Proteins / Q000737:chemistry*; D066329:Protein Aggregates; D013499:Surface Properties
chemical_list: D058228:Islet Amyloid Polypeptide; D000072002:Prion Proteins; D066329:Protein Aggregates; D006046:Gold
keywords: Ramachandran plots; SUP35; citrate-covered gold nanoparticles; hIAPP; molecular dynamics simulations