title: The effects of low-level laser irradiation on breast tumor in mice and the expression of Let-7a, miR-155, miR-21, miR125, and miR376b.
abstract: Low-level laser therapy (LLLT) is a form of photon therapy which can be a non-invasive therapeutic procedure in cancer therapy using low-intensity light in the range of 450-800 nm. One of the main functional features of laser therapy is the photobiostimulation effects of low-level lasers on various biological systems including altering DNA synthesis and modifying gene expression, and stopping cellular proliferation. This study investigated the effects of LLLT on mice mammary tumor and the expression of Let-7a, miR155, miR21, miR125, and miR376b in the plasma and tumor samples. Sixteen mice were equally divided into four groups including control, and blue, green, and red lasers at wavelengths of 405, 532, and 632 nm, respectively. Weber Medical Applied Laser irradiation was carried out with a low power of 1-3 mW and a series of 10 treatments at three times a week after tumor establishment. Tumor volume was weekly measured by a digital vernier caliper, and qRT-PCR assays were performed to accomplish the study. Depending on the number of groups and the p value of the Kolmogorov-Smirnov test of normality, a t test, a one-way ANOVA, or a non-parametric test was used for data analyses, and p < 0.05 was considered to be statistically significant. The average tumor volume was significantly less in the treated blue group than the control group on at days 21, 28, and 35 after cancerous cell injection. Our data also showed an increase of Let-7a and miR125a expression and a decrease of miR155, miR21, and miR376b expression after LLLT with the blue laser both the plasma and tumor samples compared to other groups. It seems that the non-invasive nature of laser bio-stimulation can make LLLT an attractive alternative therapeutic tool.
authors: Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
authors: Vahid
authors: V
authors: Khori
authors: Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran, Zip Code: 1419733141. aalizadeh@sina.tums.ac.ir.
authors: Ali Mohammad
authors: AM
authors: Alizadeh
authors: Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran, Zip Code: 1419733141.
authors: Zohre
authors: Z
authors: Gheisary
authors: Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran, Zip Code: 1419733141.
authors: Sadaf
authors: S
authors: Farsinejad
authors: Medical Engineering, Faculty of Biomedical Engineering, Amir Kabir University, Tehran, Iran.
authors: Farrokh
authors: F
authors: Najafi
authors: Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran, Zip Code: 1419733141.
authors: Solmaz
authors: S
authors: Khalighfard
authors: Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran, Zip Code: 1419733141.
authors: Fatemeh
authors: F
authors: Ghafari
authors: Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran, Zip Code: 1419733141.
authors: Maryam
authors: M
authors: Hadji
authors: Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran, Zip Code: 1419733141.
authors: Hamid
authors: H
authors: Khodayari
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000532:radiotherapy*; D049109:Cell Proliferation; D015870:Gene Expression; D028022:Low-Level Light Therapy / Q000379:methods*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; C550324:Mirn128 microRNA, mouse; C532623:Mirn155 microRNA, mouse; C531950:MIRN21 microRNA, mouse
keywords: Breast tumor; Low-level laser; Mice; MicroRNA