title: STAT3-induced WDR1 overexpression promotes breast cancer cell migration.
abstract: WD repeat domain 1 (WDR1), a protein that assists cofilin-mediated actin filament disassembly, is overexpressed in the invading front of invasive ductal carcinoma (IDC), but its implication of overexpression and how to be regulated have not been studied. In our study, we demonstrated that STAT3 bound to the 5' upstream sequence (-1971 to -1964), a putative promoter region, of WDR1 gene, and its activation induced WDR1 overexpression in breast cancer cells. The exogenous overexpression of WDR1 increased the migration of MDA-MB-231, which was attenuated by WDR1 knockdown. In the analysis of breast cancer patients, WDR1 overexpression was associated with a shorter distant metastasis-free survival (DMFS), more specifically in basal-like tumors.
authors: Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Republic of Korea; Department of Pathology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors: Joo Hyun
authors: JH
authors: Lee
authors: Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Republic of Korea; Department of Pathology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors: Ji Eun
authors: JE
authors: Kim
authors: Department of Pathology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors: Baek Gil
authors: BG
authors: Kim
authors: Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Republic of Korea; Department of Pathology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors: Hyun Ho
authors: HH
authors: Han
authors: The Severance Biomedical Science Institute, Seoul, Republic of Korea.
authors: Suki
authors: S
authors: Kang
authors: Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Republic of Korea; Department of Pathology, Yonsei University College of Medicine, Seoul, Republic of Korea; The Severance Biomedical Science Institute, Seoul, Republic of Korea. Electronic address: cho1988@yuhs.ac.
authors: Nam Hoon
authors: NH
authors: Cho
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D016019:Survival Analysis; D015854:Up-Regulation
chemical_list: D008840:Microfilament Proteins; D050796:STAT3 Transcription Factor; C118067:WDR1 protein, human
keywords: Breast cancer; Cell migration; Promoter binding; STAT3; Transcription factor; WDR1 (AIP1)