title: The Aggregation Pheromone of Phyllotreta striolata (Coleoptera: Chrysomelidae) Revisited.
abstract: Aggregations of the striped flea beetle Phyllotreta striolata on their crucifer host plants are mediated by volatiles emitted from feeding males. The male-specific sesquiterpene, (6R,7S)-himachala-9,11-diene (compound A), was shown previously to be physiologically and behaviorally active, but compound A was attractive only when combined with unnaturally high doses of the host plant volatile allyl isothiocyanate (AITC) in field trapping experiments. This indicated that our understanding of the chemical communication in this species is incomplete. Another male-specific sesquiterpenoid, (3S,9R,9aS)-3-hydroxy-3,5,5,9-tetramethyl-5,6,7,8,9,9a-hexahydro-1H-benzo[7]annulen-2(3H)-one (compound G), has been reported from an American P. striolata population. We confirmed the presence of compound G, and investigated its interaction with compound A and AITC in a P. striolata population in Taiwan. Compound G was attractive to Taiwanese P. striolata in laboratory bioassays, but significantly more beetles were attracted to a blend of compounds A and G. Under the same conditions, P. striolata showed no preference for the blend of A and G combined with a range of doses of AITC over the sesquiterpenoid blend alone. The sesquiterpenoid blend was tested further in field trapping experiments and attracted significantly more beetles than traps baited with compound A and ecologically relevant amounts of AITC. We conclude that A and G are components of the male-specific aggregation pheromone of P. striolata in Taiwan, and that the attractiveness of the pheromone is not reliant on the presence of AITC. Our results further indicate that the male-specific sesquiterpenoid blends differ qualitatively between the Taiwanese and American populations of P. striolata.
authors: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, D-07745, Jena, Germany. fberan@ice.mpg.de.
authors: Franziska
authors: http://orcid.org/0000-0003-2213-5347
authors: F
authors: Beran
authors: Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, D-07745, Jena, Germany.
authors: Guillermo H
authors: GH
authors: Jiménez-Alemán
authors: AVRDC-The World Vegetable Center, Entomology Unit, 60 Yi-min Liao, Shanhua, 74151, Tainan, Taiwan.
authors: Mei-Ying
authors: MY
authors: Lin
authors: AVRDC-The World Vegetable Center, Entomology Unit, 60 Yi-min Liao, Shanhua, 74151, Tainan, Taiwan.
authors: Yun-Che
authors: YC
authors: Hsu
authors: Department Urban Ecophysiology, Humboldt-Universität zu Berlin, Lentzeallee 55-57, D-14195, Berlin, Germany.|Plant Analysis and Stored Product Protection, Institute for Ecological Chemistry, Julius Kühn-Institut, Königin-Luise-Str. 19, D-14195, Berlin, Germany.
authors: Inga
authors: I
authors: Mewis
authors: AVRDC-The World Vegetable Center, Entomology Unit, 60 Yi-min Liao, Shanhua, 74151, Tainan, Taiwan.
authors: Ramasamy
authors: R
authors: Srinivasan
authors: Department Urban Ecophysiology, Humboldt-Universität zu Berlin, Lentzeallee 55-57, D-14195, Berlin, Germany.
authors: Christian
authors: C
authors: Ulrichs
authors: Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, D-07745, Jena, Germany.
authors: Wilhelm
authors: W
authors: Boland
authors: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, D-07745, Jena, Germany.
authors: Bill S
authors: BS
authors: Hansson
authors: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, D-07745, Jena, Germany.|Behavioural Ecology and Evolutionary Genetics, Max Planck Institute for Ornithology, Eberhard-Gwinner-Str. 7, 82319, Seewiesen, Germany.
authors: Andreas
authors: A
authors: Reinecke
mesh_terms: D000374:Aggression / Q000187:drug effects*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001681:Biological Assay; D001517:Coleoptera / Q000187:drug effects*; D004347:Drug Interactions; D017879:Isothiocyanates / Q000494:pharmacology; D008297:Male; D010675:Pheromones / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D012727:Sex Characteristics
chemical_list: C000609685:(6R,7S)-himachala-9,11-diene; D017879:Isothiocyanates; D010675:Pheromones; D012717:Sesquiterpenes; C004471:allyl isothiocyanate
keywords: (3S,9R,9aS)-3-hydroxy-3,5,5,9-tetramethyl-5,6,7,8,9,9a-hexahydro-1H-benzo[7]annulen-2(3H)-one; (6R,7S)-himachala-9,11-diene; Aggregation pheromone; Allyl isothiocyanate; Flea beetle; Phyllotreta striolata; Sesquiterpene