title: Electrostatically promoted dynamic hybridization of glucans with cationic polythiophene.
abstract: Hybridizing natural macromolecules with synthetic polymers is an efficient general method for constructing sophisticated supramolecular architectures. To comprehensively elucidate the controversial hybridization mechanism of glucans with synthetic polymers, the hybridization behaviors of triple-stranded curdlan (Cur) and schizophyllan (SPG) with cationic polythiophene (PyPT) were investigated in aqueous DMSO solutions by using UV-vis, circular dichroism (CD), fluorescence, fluorescence excitation, and NMR spectroscopy methods, as well as theoretical calculations, dynamic light scattering, and zeta potential measurements. Upon mixing with glucan, a hetero-triplex formed, which was dynamic and greatly accelerated by heating and by adding a base or a salt. The hetero-triplex disassembled into a hetero-duplex in highly basic solutions. Thus, polycationic polymers, such as PyPT, are expected to serve as a versatile tool for unzipping glucan homo-triplexes and promoting subsequent hybridization in aqueous solution, while the detailed mechanism elucidated in the present study contributes to the rational design of hybridization partners.
authors: Department of Applied Chemistry, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan. gaku@chem.eng.osaka-u.ac.jp.
authors: Gaku
authors: G
authors: Fukuhara
authors: Department of Applied Chemistry, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan. gaku@chem.eng.osaka-u.ac.jp.
authors: Mami
authors: M
authors: Imai
authors: Department of Chemistry, University of Victoria, PO Box 3065, Victoria, British Columbia V8 W 3V6, Canada and Laboratorio de Estructuras Biosupramoleculares, Facultad de Química, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors: Denis
authors: D
authors: Fuentealba
authors: Department of Applied Chemistry, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan. gaku@chem.eng.osaka-u.ac.jp.
authors: Yuki
authors: Y
authors: Ishida
authors: Department of Applied Chemistry, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan. gaku@chem.eng.osaka-u.ac.jp.
authors: Hiroki
authors: H
authors: Kurohara
authors: Department of Applied Chemistry, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan. gaku@chem.eng.osaka-u.ac.jp and Key Lab of Green Chemistry and Technology, College of Chemistry, Sichuan University, Wangjiang Road, Chengdu 610064, China.
authors: Cheng
authors: C
authors: Yang
authors: Department of Applied Chemistry, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan. gaku@chem.eng.osaka-u.ac.jp.
authors: Tadashi
authors: T
authors: Mori
authors: Department of Applied Chemistry, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan. gaku@chem.eng.osaka-u.ac.jp.
authors: Hiroshi
authors: H
authors: Uyama
authors: Department of Chemistry, University of Victoria, PO Box 3065, Victoria, British Columbia V8 W 3V6, Canada.
authors: Cornelia
authors: C
authors: Bohne
authors: Department of Applied Chemistry, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan. gaku@chem.eng.osaka-u.ac.jp.
authors: Yoshihisa
authors: Y
authors: Inoue
mesh_terms: D002236:Carbohydrate Conformation; D005936:Glucans / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D011108:Polymers / Q000737:chemistry*; D055672:Static Electricity; D013696:Temperature; D013876:Thiophenes / Q000737:chemistry*
chemical_list: D005936:Glucans; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene