title: CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells.
abstract: Cellular lysates from PPD+donors have been reported to transfer tuberculin reactivity to naïve recipients, but not diphtheria reactivity, and vice versa. A historically controversial topic, the terms "transfer factor" and "DLE" were used to characterize the reactivity-transferring properties of lysates. Intrigued by these reported phenomena, we found that the cellular extract derived from antigen-specific memory CD8+T cells induces IL-6 from antigen-matched APCs. This ultimately elicits IL-17 from bystander memory CD8+T cells. We have identified that dialyzable peptide sequences, S100a9, and the TCR β chain from CD8+T cells contribute to the molecular nature of this activity. We further show that extracts from antigen-targeted T cells enhance immunity to Staphylococcus aureus and Candida albicans These effects are sensitive to immunization protocols and extraction methodology in ways that may explain past discrepancies in the reproducibility of passive cellular immunity.
authors: Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA; mylesi@niaid.nih.gov.
authors: Ian A
authors: IA
authors: Myles
authors: Research Technologies Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors: Ming
authors: M
authors: Zhao
authors: Research Technologies Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors: Glenn
authors: G
authors: Nardone
authors: Research Technologies Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors: Lisa R
authors: LR
authors: Olano
authors: Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors: Jensen D
authors: JD
authors: Reckhow
authors: Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors: Danial
authors: D
authors: Saleem
authors: Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors: Timothy J
authors: TJ
authors: Break
authors: Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors: Michail S
authors: MS
authors: Lionakis
authors: Genomic Technologies Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA; and.
authors: Timothy G
authors: TG
authors: Myers
authors: Genomic Technologies Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA; and.
authors: Paul J
authors: PJ
authors: Gardina
authors: University of Colorado Health Science Center, Denver, Colorado, USA.
authors: Charles H
authors: CH
authors: Kirkpatrick
authors: Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors: Steven M
authors: SM
authors: Holland
authors: Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors: Sandip K
authors: SK
authors: Datta
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D003956:Dialysis; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007109:Immunity; D007156:Immunologic Memory; D020381:Interleukin-17 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D013154:Spleen / Q000473:pathology
chemical_list: D000941:Antigens; D000939:Epitopes; D020381:Interleukin-17; D011948:Receptors, Antigen, T-Cell; D009418:S100 Proteins
keywords: Tc17; cellular immunity; transfer factor