title: Bioconversion of waste office paper to hydrogen using pretreated rumen fluid inoculum.
abstract: In this study, a microbial consortium from an acid-treated rumen fluid was used to improve the yields of H2production from paper residues in batch reactors. The anaerobic batch reactors, which contained paper and cellulose, were operated under three conditions: (1) 0.5 g paper/L, (2) 2 g paper/L, and (3) 4 g paper/L. Cellulase was added to promote the hydrolysis of paper to soluble sugars. The H2yields were 5.51, 4.65, and 3.96 mmol H2/g COD, respectively, with substrate degradation ranging from 56 to 65.4 %. Butyric acid was the primary soluble metabolite in the three reactors, but pronounced solventogenesis was detected in the reactors incubated with increased paper concentrations (2.0 and 4.0 g/L). A substantial prevalence of Clostridium acetobutylicum (99 % similarity) was observed in the acid-treated rumen fluid, which has been recognized as an efficient H2-producing strain in addition to ethanol and n-butanol which were also detected in the reactors.
authors: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. João Dagnone, 1100, Jd. Santa Angelina, 13563-120, São Carlos, SP, Brazil.
authors: Lívia Silva
authors: LS
authors: Botta
authors: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. João Dagnone, 1100, Jd. Santa Angelina, 13563-120, São Carlos, SP, Brazil.
authors: Regiane Priscila
authors: RP
authors: Ratti
authors: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. João Dagnone, 1100, Jd. Santa Angelina, 13563-120, São Carlos, SP, Brazil.
authors: Isabel Kimiko
authors: IK
authors: Sakamoto
authors: Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luis, Km 235, 13565-905, São Carlos, SP, Brazil.
authors: Lucas Rodrigues
authors: LR
authors: Ramos
authors: Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luis, Km 235, 13565-905, São Carlos, SP, Brazil. edsilva@ufscar.br.
authors: Edson Luiz
authors: EL
authors: Silva
authors: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. João Dagnone, 1100, Jd. Santa Angelina, 13563-120, São Carlos, SP, Brazil.
authors: Maria Bernadete Amâncio
authors: MB
authors: Varesche
mesh_terms: D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006868:Hydrolysis; D010209:Paper; D062611:Solid Waste
chemical_list: D062611:Solid Waste; D006859:Hydrogen; D002482:Cellulose
keywords: Acid pretreatment; Anaerobic consortium; Cellulase; Clostridium acetobutylicum; Solventogenesis