title: Immobilization of invertase on chitosan coated γ-Fe2O3 magnetic nanoparticles to facilitate magnetic separation.
abstract: Industrially important invertase enzyme was immobilized on chitosan coated sol gel derived γ-Fe2O3 magnetic nanoparticles (MNPs) to enable it for repetitive use by magnetic separation. MNPs were characterized by X-ray diffraction (XRD), dynamic light scattering (DLS), field emission scanning electron microscope (FE-SEM), Fourier transform infrared (FTIR) spectrometer and magnetic measurements. FTIR studies confirmed successful immobilization of invertase on MNPs. The ability to convert sucrose into invert syrup was enhanced in immobilized invertase compared to that of free enzyme. Further it was found that invertase immobilized on MNPs (IIMNPs) were more stable at varying pH and temperature conditions. Magnetic separation technique was successfully employed for reuse of the IIMNPs for 20 times without significant loss of activity.
authors: Thin Film Materials Laboratory, Department of Physics, Shivaji University Kolhapur, MS, India.
authors: P P
authors: PP
authors: Waifalkar
authors: Department of Chemistry, The New College, Kolhapur, MS, India.
authors: S B
authors: SB
authors: Parit
authors: Department of Chemistry, The New College, Kolhapur, MS, India.
authors: A D
authors: AD
authors: Chougale
authors: Department of Physics, Central University of Kerala, Kasaragod, KL, India.
authors: Subasa C
authors: SC
authors: Sahoo
authors: Thin Film Materials Laboratory, Department of Physics, Shivaji University Kolhapur, MS, India. Electronic address: psp_phy@unishivaji.ac.in.
authors: P S
authors: PS
authors: Patil
authors: Department of Physics, The New College, Kolhapur, MS, India. Electronic address: prashantphy@gmail.com.
authors: P B
authors: PB
authors: Patil
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D017743:Equipment Reuse; D005290:Ferric Compounds / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D059346:Magnets; D044367:Phase Transition; D013395:Sucrose / Q000737:chemistry; D013696:Temperature; D043324:beta-Fructofuranosidase / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D013395:Sucrose; D048271:Chitosan; D043324:beta-Fructofuranosidase
keywords: Invertase; Magnetic properties; Magnetic separation; Sol–gel processes