title: Cyclin-Dependent Kinase 14 Promotes Cell Proliferation, Migration and Invasion in Ovarian Cancer by Inhibiting Wnt Signaling Pathway.
abstract: OBJECTIVE
  The study aimed to investigate cyclin-dependent kinase 14 (CDK14) and its co-function with Wnt signaling pathway on cell proliferation, migration and invasion in ovarian cancer.
  
  
  METHODS
  CDK14 expressions were detected by quantitative real-time polymerase chain reaction. The expressions c-Myc, cyclinD1, PFTK1, ki67 and OGT were examined by Western blot. MTT assay was applied to observe cell proliferation after transfection of pEGFP-N1/CDK14-siRNA and pEGFP-N1 into SKOV3 cells, and scratch test and Transwell assay to observe invasion and migration ability. Transfected tumor model in nude mice was established.
  
  
  RESULTS
  CDK14 was upregulated in the ovarian cancer tissues and cell lines (both p < 0.05). Expressions of downstream molecules in Wnt signaling pathway as well as the proliferation, invasion and migration ability of the SKOV3 cells were reduced when CDK14 was inhibited (all p < 0.05). The expression of β-catenin in the nucleus was also decreased when CDK14 was inhibited (p < 0.05). In the transfected tumor model of nude mice, the results showed, compared with the pEGFP-N1 group and blank control group, that the expressions of c-Myc, cyclinD1, PFTK1, ki67 and OGT in the pEGFP-N1/CDK14-siRNA group in the transplantation tumor tissues decreased significantly (all p < 0.05).
  
  
  CONCLUSIONS
  CDK14 suppression-mediated Wnt signaling pathway can inhibit cell proliferation, invasion and migration in ovarian cancer.
authors: Department of Obstetrics and Gynecology, Tongji Medical College Affiliated Puai Hospital to Huazhong University of Science and Technology, Wuhan, Hubei, PR China.
authors: Jun
authors: J
authors: Ou-Yang
authors: Li-Hong
authors: LH
authors: Huang
authors: Xiang-Xiu
authors: XX
authors: Sun
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D002467:Cell Nucleus; D049109:Cell Proliferation; D018844:Cyclin-Dependent Kinases / Q000502:physiology*; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000473:pathology*; D034741:RNA, Small Interfering; D014162:Transfection; D060449:Wnt Signaling Pathway / Q000502:physiology*
chemical_list: D034741:RNA, Small Interfering; C515076:CDK14 protein, human; D018844:Cyclin-Dependent Kinases
keywords: Cell invasion; Cell migration; Cell proliferation; Cyclin-dependent kinase 14; Ovarian cancer; SKOV3; Wnt signaling pathway; β-Catenin