title: Detection of Felis catus gammaherpesvirus 1 (FcaGHV1) in peripheral blood B- and T-lymphocytes in asymptomatic, naturally-infected domestic cats.
abstract: The domestic cat is natural host to both feline immunodeficiency virus and Felis catus gammaherpesvirus 1 (FcaGHV1). Comparative data suggest that these agents might act as synergistic copathogens in feline AIDS-related lymphoma. To identify leucocyte subsets harbouring gammaherpesvirus DNA, whole blood from 5 healthy, FcaGHV1-infected cats was labelled with monoclonal antibodies to feline CD21, CD4, CD8 and CD14 for 4-way fluorescence-activated cell sorting. FcaGHV1gB qPCR was performed on DNA extracted from purified fractions and whole blood longitudinally. FcaGHV1 DNA was detected in CD21+, CD4+, CD8+, but not CD14+ cells. Variation in whole blood load, up to 19,788 copies/10(6)cells, was detected in individual cats over time. FcaGHV1 DNA was undetectable in one cat on one occasion highlighting that qPCR of whole blood from a single time point will not detect all cases of FcaGHV1 infection. Further investigation of the role of FcaGHV1 in feline lymphoid malignancies is warranted.
authors: Faculty of Veterinary Science and Marie Bashir Institute for Infectious Diseases and Biosecurity, School of Life and Environmental Sciences, The University of Sydney, NSW 2006, Australia.
authors: Alicia J
authors: AJ
authors: McLuckie
authors: Faculty of Veterinary Science and Marie Bashir Institute for Infectious Diseases and Biosecurity, School of Life and Environmental Sciences, The University of Sydney, NSW 2006, Australia.
authors: Vanessa R
authors: VR
authors: Barrs
authors: Advanced Cytometry Facility, University of Sydney/Centenary Institute, NSW 2006, Australia.
authors: Adrian L
authors: AL
authors: Smith
authors: Faculty of Veterinary Science and Marie Bashir Institute for Infectious Diseases and Biosecurity, School of Life and Environmental Sciences, The University of Sydney, NSW 2006, Australia. Electronic address: julia.beatty@sydney.edu.au.
authors: Julia A
authors: JA
authors: Beatty
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000821:virology*; D002371:Cat Diseases / Q000276:immunology / Q000821:virology*; D002415:Cats; D004279:DNA, Viral; D018142:Gammaherpesvirinae / Q000502:physiology*; D006566:Herpesviridae Infections / Q000662:veterinary*; D016130:Immunophenotyping; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000821:virology*; D019562:Viral Load
chemical_list: D000954:Antigens, Surface; D004279:DNA, Viral
keywords: Cat; Feline; Gammaherpesvirus; Leucocyte; Virus load