title: Significance of oxygen carriers and role of liquid paraffin in improving validamycin A production.
abstract: Validamycin A (Val-A) synthesized by Streptomyces hygroscopicus 5008 is widely used as a high-efficient antibiotic to protect plants from sheath blight disease. A novel fermentation strategy was introduced to stimulate Val-A production by adding oxygen carriers. About 58 % increase in Val-A production was achieved using liquid paraffin. Further, biomass, carbon source, metabolic genes, and metabolic enzymes were studied. It was also found that the supplementation of liquid paraffin increased the medium dissolved oxygen and intracellular oxidative stress level. The expression of the global regulators afsR and soxR sensitive to ROS, ugp catalyzing synthesis of Val-A precursor, and Val-A structural genes was enhanced. The change of the activities of glucose-6-phosphate dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase was observed, which reflected the redirection of carbon metabolic flux. Based on these results, liquid paraffin addition as an oxygen carrier could be a useful technique in industrial production of Val-A and our study revealed a redox-based secondary metabolic regulation in S. hygroscopicus 5008, which provided a new insight into the regulation of the biosynthesis of secondary metabolites.
authors: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
authors: Jinsong
authors: J
authors: Feng
authors: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
authors: Jing
authors: J
authors: Jiang
authors: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
authors: Yan
authors: Y
authors: Liu
authors: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
authors: Wei
authors: W
authors: Li
authors: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
authors: Ramila
authors: R
authors: Azat
authors: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
authors: Xiaodong
authors: X
authors: Zheng
authors: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou, 310058, Zhejiang, China. vivianzhou11@zju.edu.cn.
authors: Wen-Wen
authors: WW
authors: Zhou
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D005285:Fermentation; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D007294:Inositol / Q000031:analogs & derivatives* / Q000096:biosynthesis; D008899:Mineral Oil; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; C030790:validamycin A; D007294:Inositol; D008899:Mineral Oil; D005954:Glucosephosphate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010100:Oxygen
keywords: Fermentation strategy; Liquid paraffin; Oxygen carrier; Streptomyces hygroscopicus; Validamycin A