title: Application of Cookson-type reagents for biomedical HPLC and LC/MS analyses: a brief overview.
abstract: High-performance liquid chromatography (HPLC), LC/mass spectrometry (MS) and LC/tandem mass spectrometry (MS/MS) have been widely used for biomedical analyses, in which chemical derivatization is one of the most important methods to increase the sensitivity and selectivity. A Cookson-type reagent [4-substituted-1,2,4-triazoline-3,5-dione (4-substituted-TAD)] reacts with the compound bearing a conjugated diene, such as the vitamin D compound, to quantitatively form the stable Diels-Alder adduct. The reagent with a chromophore or fluorophore at the 4-position of TAD yields a highly responsive adduct for the UV or fluorescence detection, respectively. The Diels-Alder adduct with a Cookson-type reagent having a permanently charged, proton-affinitive or electron-affinitive moiety is sensitively detected by a specific MS analyzer. This paper is a brief overview of the applications of the reagents for biomedical analyses mainly using HPLC or LC/MS(/MS).
authors: Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, 278-8510, Japan.
authors: Tatsuya
authors: T
authors: Higashi
authors: Professor Emeritus of Kanazawa University, Japan.
authors: Kazutake
authors: K
authors: Shimada
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D061565:Cycloaddition Reaction; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014230:Triazoles / Q000737:chemistry; D014807:Vitamin D / Q000032:analysis* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D014230:Triazoles; C068159:4-methyl-1,2,4-triazoline-3,5-dione; D014807:Vitamin D
keywords: Cookson-type reagent; Diels-Alder adduct; conjugated diene; derivatization; liquid chromatography; mass spectrometry