title: Use of antioxidants in the analysis of vatamins A and E in mammalian plasma by high performance liquid chromatography.
abstract: A simple, sensitive, quantitative method for the simultaneous assay of retinol, α-tocopherol and γ-tocopherol in rat, guinea pig, monkey and human plasma was developed by using high performance liquid chromatography. It was found that antioxidant was required to stabilize the fat-soluble vitamins in the plasma of rats. The effect of several antioxidants on the recovery of fat-soluble vitamins was evaluated. Results showed that 0.125% butylated hydroxytoluene (BHT) in ethanol and 0.025% BHT in heptane yielded recoveries >95% in 0.1 ml plasma.
authors: Nutrients Research Unit, US Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 94710, Berkeley, CA.
authors: F I
authors: FI
authors: Chow
authors: Nutrients Research Unit, US Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 94710, Berkeley, CA.
authors: S T
authors: ST
authors: Omeye