title: hsp90 and hsp47 appear to play an important role in minnow Puntius sophore for surviving in the hot spring run-off aquatic ecosystem.
abstract: Changes in the expression of a number of hsp genes in minnow Puntius sophore collected from a hot spring run-off (Atri hot spring in Odisha, India; 20o09'N 85°18'E, 36-38 °C) were investigated to study the upper thermal acclimation response under heat stress, using same species from aquaculture ponds (water temperature 27 °C) as control. Expression of hsp genes was analyzed in both groups using RT-qPCR, which showed up-regulation of hsp90 (2.1-fold) and hsp47 (2.5-fold) in hot spring run-off fishes, whereas there was no alteration in expression of other hsps. As the fish inhabit the hot spring run-off area for very long duration, they could have adapted to the environment. To test this hypothesis, fishes collected from hot spring run-off were divided into two groups; one was heat-shocked at 41 °C/24 h, and the other was acclimatized at 27 °C/24 h. Up-regulation of all the hsps (except hsp78) was observed in the heat-shocked fishes, whereas expression of all hsps was found to be down-regulated to the basal level in fishes maintained at 27 °C/24 h. Pathway analysis showed that the expressions of all the hsps except hsp90 are regulated by the transcription factor heat shock factor 1 (Hsf1). This study showed that hsp90 and hsp47 play an important role in Puntius sophore for surviving in the high-temperature environment of the hot spring run-off. Additionally, we show that plasticity in hsp gene expression is not lost in the hot spring run-off population.
authors: Biochemistry Laboratory, Proteomics Unit, Fishery Resource and Environmental Management Division, ICAR- Central Inland Fisheries Research Institute, Barrackpore, Kolkata, 700120, India.|KIIT School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.
authors: Arabinda
authors: A
authors: Mahanty
authors: KIIT School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.
authors: Gopal Krishna
authors: GK
authors: Purohit
authors: KIIT School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.
authors: Ravi Prakash
authors: RP
authors: Yadav
authors: KIIT School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.
authors: Sasmita
authors: S
authors: Mohanty
authors: Biochemistry Laboratory, Proteomics Unit, Fishery Resource and Environmental Management Division, ICAR- Central Inland Fisheries Research Institute, Barrackpore, Kolkata, 700120, India. bimal.mohanty@icar.gov.in.
authors: Bimal Prasanna
authors: BP
authors: Mohanty
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000235:genetics*; D017753:Ecosystem; D020869:Gene Expression Profiling; D050958:HSP47 Heat-Shock Proteins / Q000235:genetics*; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response; D045482:Hot Springs / Q000032:analysis; D006358:Hot Temperature; D008670:Metals / Q000032:analysis; D015854:Up-Regulation
chemical_list: D050958:HSP47 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D008670:Metals
keywords: Gene plasticity; Heat stress; RT-qPCR; Thermal acclimation; hsp gene