title: Micro RNA-19a suppresses IL-10 in peripheral B cells from patients with atherosclerosis.
abstract: The interleukin (IL)-10-production B cells play an important role in the pathogenesis of atherosclerosis (Asro) with unknown mechanism. Micro RNA (miR)-17-92 cluster has strong immune regulatory activities. This study tests a hypothesis that miR-17-92 cluster suppresses IL-10 expression in B cells of Asro patients.
  
  
  
  Patients with Asro were recruited into this study. Peripheral blood samples were collected from the patients. B cells were isolated from the blood samples and analyzed to elucidate the role of miR-17-92 in the regulation of IL-10 expression.
  
  
  
  Peripheral B cells from patients with Asro show lower levels of IL-10 than that from healthy subjects. The IL-10 expression in the B cells is negatively correlated with the expression of miR-19a in the B cells. The serum levels of tumor necrosis factor (TNF)-α, interferon (IFN)-γ and interleukin (IL)-4 in Asro patients were higher than healthy subjects. Exposure to TNF-α or IFN-γ or IL-4 suppressed IL-10 expression in B cells via increasing the expression of miR-19a in B cells, which could be abolished by Inhibition of miR-19a.
  
  
  
  TNF-α or IFN-γ or IL-4 suppresses IL-10 in B cells via up regulating miR-19a expression.
authors: Department of Emergency, The Fourth Clinical Hospital of Harbin Medical University, Harbin 150001, China.
authors: Zhong-Qiao
authors: ZQ
authors: Ren
authors: Department of Emergency, The Fourth Clinical Hospital of Harbin Medical University, Harbin 150001, China.
authors: Ning
authors: N
authors: Liu
authors: Department of Emergency, The Fourth Clinical Hospital of Harbin Medical University, Harbin 150001, China. Electronic address: kanrzhao@sina.com.
authors: Kan
authors: K
authors: Zhao
mesh_terms: D050197:Atherosclerosis / Q000097:blood / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000037:antagonists & inhibitors* / Q000097:blood / Q000235:genetics* / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D034622:RNA Interference; D062085:RNA, Long Noncoding; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: C508609:IL10 protein, human; C508598:IL4 protein, human; C568997:MIR17HG, human; C549214:MIRN19A microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: Atherosclerosis; Interleukin-10; Lymphocyte; Micro RNA; p300