title: Preventing further misuse of the ELISA technique and misinterpretation of serological antibody assay data.
abstract: The indirect ELISA is a widely utilized method to assay serum antibodies. However, a common and critical problem when analyzing serum antibodies is the disregard for the background noise reaction caused by the hydrophobic binding of immunoglobulin components in serum components to plastic. Unfortunately, current blocking agents cannot prevent this background noise reaction. To prevent further misuse of the ELISA technique, it is important to openly discuss the fundamental problems involved in the ELISA system.
authors: Chondrex, Inc., 2607 151st Place NE, Redmond, WA 98052, United States. Electronic address: terato@chondrex.com.
authors: Kuniaki
authors: K
authors: Terato
authors: Chondrex, Inc., 2607 151st Place NE, Redmond, WA 98052, United States. Electronic address: cdo@chondrex.com.
authors: Christopher
authors: C
authors: Do
authors: Chondrex, Inc., 2607 151st Place NE, Redmond, WA 98052, United States. Electronic address: jchang@chondrex.com.
authors: Jessica
authors: J
authors: Chang
authors: Chondrex, Inc., 2607 151st Place NE, Redmond, WA 98052, United States. Electronic address: twaritani@chondrex.com.
authors: Takaki
authors: T
authors: Waritani
mesh_terms: D000906:Antibodies / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005189:False Positive Reactions; D006801:Humans; D010969:Plastics / Q000737:chemistry; D012698:Serologic Tests / Q000379:methods*; D059629:Signal-To-Noise Ratio
chemical_list: D000906:Antibodies; D010969:Plastics
keywords: Antibody; Background noise reaction; ELISA; False positive reaction; Human serum