title: LC-MS/MS assay of ropinirole in rat biological matrices: elimination of lysoglycerophosphocholines-based matrix effect.
abstract: OBJECTIVE
  To adequately support PK evaluation of ropinirole in rats following intranasal administration, it was desirable to determine ropinirole concentrations in rat plasma, brain homogenate and microdialysate. Results & methodology: A robust LC-MS/MS method has been developed for the determination of ropinirole in biological samples. Liquid-liquid extraction using ethyl acetate eliminated matrix effect due to significantly reduced levels of lysoglycerophosphocholines. The assay was fully validated with dynamic ranges of 0.01-20 ng/ml for plasma and brain homogenate samples and 0.1-200 ng/ml for microdialysate samples.
  
  
  CONCLUSIONS
  The proposed method was accurate and precise for the quantification of ropinirole in biological samples and was successfully applied to a microdialysis study of ropinirole in rats.
authors: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.|Department of Pharmacy, Chongqing General Hospital, Chongqing 400000, PR China.
authors: Xingjin
authors: X
authors: Yan
authors: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.
authors: Shouwei
authors: S
authors: Zhao
authors: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.
authors: Wenwen
authors: W
authors: Zhu
authors: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.
authors: Jing
authors: J
authors: Rao
authors: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.
authors: Li
authors: L
authors: Kang
authors: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.
authors: Jiaqiang
authors: J
authors: Xu
authors: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.
authors: Dan
authors: D
authors: Li
authors: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.
authors: Lixia
authors: L
authors: Qin
authors: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.
authors: Lihui
authors: L
authors: Qiu
authors: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.|Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.
authors: Gao
authors: G
authors: Li
authors: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.|Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.
authors: Jiangeng
authors: J
authors: Huang
authors: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.|Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan, Hubei 430030, PR China.
authors: Luqin
authors: L
authors: Si
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000978:Antiparkinson Agents / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007211:Indoles / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D059625:Liquid-Liquid Extraction; D008297:Male; D017551:Microdialysis; D010767:Phosphorylcholine / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000978:Antiparkinson Agents; D007211:Indoles; C046649:ropinirole; D010767:Phosphorylcholine
keywords: LC–MS/MS; intranasal administration; matrix effect; microdialysis; ropinirole