title: Conformational and physicochemical properties of fucosylated chondroitin sulfate from sea cucumber Apostichopus japonicus.
abstract: This study aimed at investigating the chain conformation and physicochemical properties of fucosylated chondroitin sulfate extracted from sea cucumber Apostichopus japonicas (Aj-fCS). By using HPSEC-MALLS-Visc-RI, Mw, <s(2)>z(1/2), Rh and [η] for Aj-fCS were determined as 58.0±4.4kDa, 21.8±1.3nm, 12.5±1.3nm and 27.8±0.5mL/g respectively. Conformation parameter αs derived from the relationship of Mw-<s(2)>z(1/2) (0.39) and structure-sensitive parameter ρ (1.74) consistently indicated that Aj-fCS adopted a random coil conformation in solution, which was also supported by atomic force microscopy. Stiffness parameters of Aj-fCS chains including q (2.72nm), d (1.03nm) and C∞ (5.28) were furthermore deduced from the worm-like cylinder model. Aj-fCS demonstrated a shear-thinning rheological behavior, relatively low apparent viscosity, negative charge in wide pH and ionic strength ranges, and favorable thermostability. These results have important implications for designing and fabricating functional foods or drugs based on Aj-fCS.
authors: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
authors: Xiaoqi
authors: X
authors: Xu
authors: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
authors: Changhu
authors: C
authors: Xue
authors: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. Electronic address: changyg@ouc.edu.cn.
authors: Yaoguang
authors: Y
authors: Chang
authors: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
authors: Feng
authors: F
authors: Chen
authors: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
authors: Jun
authors: J
authors: Wang
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D047528:Stichopus / Q000737:chemistry*; D014783:Viscosity
chemical_list: D002809:Chondroitin Sulfates
keywords: Chain conformation; Fucosylated chondroitin sulfate; Rheology; Sea cucumber; Thermostability; ζ-Potential