title: Identification and functional analysis of invasion associated locus B (IalB) in Bartonella species.
abstract: Bartonellosis is caused by the genus Bartonella. Bartonella is widely distributed in the ruminants, cats, dogs, rodents and other mammals including humans. At least 13 species or subspecies of Bartonella are zoonotic, and each species appears to be highly adapted to one or a limited number of reservoir animals in which it is asymptomatic, while it can be transmitted to humans in which a variety of clinical manifestations can be caused. It was reported that Bartonella henselae infection rate among domestic cats was high in nature, making it one of the leading, important, and easily neglected zoonotic diseases. The aims of this study were to identify the expression, localization, immunogenicity and functional mechanism of Bartonella virulence factor IalB. We found that recombinant IalB protein could react with the serum from infected reservoir hosts and anti-IalB polyclonal antibodies could react with different Bartonella species by western blot analysis. According to these results, we proposed that IalB protein and anti-IalB antibodies would be good candidates for diagnosis of Bartonella infection by antigen-based anti-IalB antibodies or antibody-based IalB antigen capture immunoassay, respectively. We also found that IalB had a putative 22-amino-acid signal sequence and little IalB was localized to the outer membrane of Bartonella birtlesii by electron microscopy assay. Incubation with anti-IalB polyclonal antibodies resulted in inhibition of the invasion of mouse erythrocytes by B. birtlesii. According to these results, we propose that IalB could be a secreted protein that facilitates Bartonella entry into erythrocytes. In conclusion, these results improve our understanding of IalB as a candidate for immunodiagnosis and how IalB affects Bartonella-erythrocyte entry.
authors: School of Life Sciences, Shandong University of Technology, No.12 Zhangzhou Road, 255 049 Zhangdian Zibo, Shandong Province, China; USC INRA Bartonella et Tiques, UMR BIPAR, ENVA, ANSES, UPEC, 23 Avenue du Général de Gaulle, 94700 Maisons-Alfort, France.
authors: Hongkuan
authors: H
authors: Deng
authors: School of Life Sciences, Shandong University of Technology, No.12 Zhangzhou Road, 255 049 Zhangdian Zibo, Shandong Province, China.
authors: Qiuxiang
authors: Q
authors: Pang
authors: The Animal Husbandry Bureau of Gaomi County, No.83 Fenghuang Avenue Xinyi Street, 261500 Gaomi Weifang, Shandong Province, China.
authors: Huiqing
authors: H
authors: Xia
authors: USC INRA Bartonella et Tiques, UMR BIPAR, ENVA, ANSES, UPEC, 23 Avenue du Général de Gaulle, 94700 Maisons-Alfort, France.
authors: Danielle
authors: D
authors: Le Rhun
authors: USC INRA Bartonella et Tiques, UMR BIPAR, ENVA, ANSES, UPEC, 23 Avenue du Général de Gaulle, 94700 Maisons-Alfort, France.
authors: Evelyne
authors: E
authors: Le Naour
authors: School of Life Sciences, Shandong University of Technology, No.12 Zhangzhou Road, 255 049 Zhangdian Zibo, Shandong Province, China.
authors: Chengliang
authors: C
authors: Yang
authors: USC INRA Bartonella et Tiques, UMR BIPAR, ENVA, ANSES, UPEC, 23 Avenue du Général de Gaulle, 94700 Maisons-Alfort, France. Electronic address: mvayssier@vet-alfort.fr.
authors: Muriel
authors: M
authors: Vayssier-Taussat
authors: School of Life Sciences, Shandong University of Technology, No.12 Zhangzhou Road, 255 049 Zhangdian Zibo, Shandong Province, China. Electronic address: Zhaobosheng@sdut.edu.cn.
authors: Bosheng
authors: B
authors: Zhao
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001473:Bartonella / Q000235:genetics* / Q000276:immunology*; D015153:Blotting, Western; D004705:Endocytosis; D004912:Erythrocytes / Q000382:microbiology; D005260:Female; D020869:Gene Expression Profiling; D056426:Genetic Loci; D008807:Mice, Inbred BALB C; D021381:Protein Transport; D037521:Virulence Factors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000907:Antibodies, Bacterial; D037521:Virulence Factors; D017766:Acid Anhydride Hydrolases
keywords: Bartonalla infection; Erythrocyte; Invasion associated locus B (IalB)