title: Rapid Identification and Comparison of Compounds with Antioxidant Activity in Coreopsis tinctoria Herbal Tea by High-Performance Thin-Layer Chromatography Coupled with DPPH Bioautography and Densitometry.
abstract: A simple and efficient method based on high-performance thin-layer chromatography coupled with 2,2-diphenyl-1-picrylhydrazyl (DPPH) bioautography (HPTLC-DPPH) was established for the screening and comparison of antioxidants in different parts of Coreopsis tinctoria herbal tea from different origins and other related herbal tea materials, which used Chrysanthemum morifolium cv. "Gongju" and "Hangju" in this study. Scanning densitometry after DPPH derivatization was applied for the determination of antioxidant capacities of isolated compounds in each sample. It is considered that ethanol extracts of C. tinctoria had stronger antioxidant activity and more characteristic bands than those of 2 compared samples, C. morifolium cv. "Gongju" and "Hangju." Chemometric analysis results showed that the combination of hierarchical clustering analysis and principal component analysis based on determined antioxidant capacities could be used for the discrimination of different parts of C. tinctoria and C. morifolium. Results showed that 7 compounds made up the major contributions of antioxidant activity in C. tinctoria, including okanin, isookanin, marein, flavanomarein, 5,7,3',5'-tetrahydroxyflavanone-7-O-glucoside, 3,5-dicaffeoylquinic acid, and chlorogenic acid. Therefore, 7 compounds were identified as major antioxidant biomarkers for quality control of C. tinctoria. Results demonstrated that the established method could be applied for the identification of C. tinctoria, and were beneficial for the bioactivity-based quality control of C. tinctoria.
authors: State Key Laboratory of Quality Research in Chinese Medicine, Inst. of Chinese Medical Sciences, Univ. of Macau, Macao, China.
authors: Shing-Chung
authors: SC
authors: Lam
authors: State Key Laboratory of Quality Research in Chinese Medicine, Inst. of Chinese Medical Sciences, Univ. of Macau, Macao, China.
authors: Sio-Fong
authors: SF
authors: Lam
authors: State Key Laboratory of Quality Research in Chinese Medicine, Inst. of Chinese Medical Sciences, Univ. of Macau, Macao, China. jingzhao@umac.mo.
authors: Jing
authors: J
authors: Zhao
authors: State Key Laboratory of Quality Research in Chinese Medicine, Inst. of Chinese Medical Sciences, Univ. of Macau, Macao, China.
authors: Shao-Ping
authors: SP
authors: Li
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D047188:Chalcones / Q000032:analysis / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D002855:Chromatography, Thin Layer / Q000379:methods; D031202:Chrysanthemum / Q000737:chemistry; D031945:Coreopsis / Q000737:chemistry*; D003720:Densitometry; D044950:Flavanones / Q000032:analysis / Q000494:pharmacology; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011786:Quality Control; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D000069459:Teas, Herbal / Q000032:analysis
chemical_list: C479303:5,7,3',5'-tetrahydroxyflavanone; D000975:Antioxidants; D001713:Biphenyl Compounds; D047188:Chalcones; D044950:Flavanones; D010851:Picrates; D010936:Plant Extracts; D000069459:Teas, Herbal; C472707:caffeoylquinic acid; C577175:isookanin; C550306:marein; C550307:okanin; D011801:Quinic Acid; D002726:Chlorogenic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; C100434:3,5-dicaffeoylquinic acid
keywords: Coreopsis tinctoria; HPTLC-DPPH; antioxidant activity; herbal tea; identification