title: Highly Efficient Synthesis of Fructooligosaccharides by Extracellular Fructooligosaccharide-Producing Enzymes and Immobilized Cells of Aspergillus aculeatus M105 and Purification and Biochemical Characterization of a Fructosyltransferase from the Fungus.
abstract: In this work, Aspergillus aculeatus M105 was obtained to produce high extracellular fructooligosaccharide-producing enzyme activity. The maximum yields of fructooligosaccharides produced by its extracellular enzymes and immobilized cells were 67.54 and 65.47% (w/w), respectively. A fructosyltransferase (FTase), AaFT32A, was purified from M105. The optimal pH and temperature of AaFT32A were pH 5.0-6.0 and 65 °C, respectively. The Km, Vmax, and kcat values for the transfructosylating activity of AaFT32A were 2267 mM, 1347 μmol/min/mg protein, and 1550.2 s(-1), respectively, and those values for the hydrolytic activity of AaFT32A were 6.10 mM, 32.44 μmol/min/mg protein, and 37.3 s(-1), respectively. The sequence of AaFT32A deduced from the cloned gene shared 99.4% identity with a FTase from Aspergillus japonicus CB05 and a fructofuranosidase from Aspergillus niger and 96.5% identity with a FTase (Aspacl_37092) from A. aculeatus ATCC 16872. The fungal strain and its FTase may have potential applications in the prebiotics industry.
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University , 100 Daxue Road, Nanning 530004, China.
authors: Mei-Ping
authors: MP
authors: Huang
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University , 100 Daxue Road, Nanning 530004, China.
authors: Min
authors: M
authors: Wu
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University , 100 Daxue Road, Nanning 530004, China.
authors: Qiang-Sheng
authors: QS
authors: Xu
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University , 100 Daxue Road, Nanning 530004, China.
authors: De-Jiao
authors: DJ
authors: Mo
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University , 100 Daxue Road, Nanning 530004, China.
authors: Jia-Xun
authors: JX
authors: Feng
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D018914:Cells, Immobilized; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006602:Hexosyltransferases / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000096:biosynthesis*; D013696:Temperature; D001426:Bacterial Proteins
chemical_list: D005656:Fungal Proteins; D006602:Hexosyltransferases; D009844:Oligosaccharides; C116580:fructooligosaccharide; C021451:inulosucrase; D001426:Bacterial Proteins
keywords: Aspergillus aculeatus; fructooligosaccharide-producing enzyme; fructooligosaccharides; fructosyltransferase; immobilized cells