title: High-performance Liquid Chromatography Measured Metabolites of Endogenous Catecholamines and Their Relations to Chronic Kidney Disease and High Blood Pressure in Heart Transplant Recipients.
abstract: BACKGROUND
  Patients after solid organ transplantation, especially heart and kidneys, are prone to be hypertensive. Recently chronic kidney disease and renalase metabolism of endogenous catecholamines are thought to make major contribution to the pathogenesis of hypertension.
  
  
  METHODS
  We analyzed 75 heart recipients (80% male, 20% female), medium age 54.9 years (range, 25-75) at 0.5 to 22 years after heart transplantation (median, 10.74). Diagnosis of hypertension was made on the basis of ambulatory blood pressure monitoring. Complete blood count, urea, creatinine, estimated glomerular filtration rate (eGFR), renalase in serum, and levels of metanefrine, normetanefrine, and 3-metoxytyramine in 24-hour urine collection calculated with a high-performance liquid chromatography were recorded.
  
  
  RESULTS
  Urine endogenous catecholamine metabolites were estimated according to creatinine clearance. Normetanefrine was correlated with age (r = 0.27; P < .05), urea (r = 0.64; P < .01), creatinine (r = 0.6; P < .01), eGFR (r = -0.51; P < .01), renalase (r = 0.5; P < .01), and diastolic blood pressure (r = 0.26; P < .05). Metanefrine was correlated with urea (r = 0.43; P < .01), creatinine (0.32; P < .01), eGFR (r = -0.4; P < .01), renalase (r = 0.34; P < .05), height (r = -0.26; P < .05), weight (r = -0.23; P < .05), and time after heart transplantation (r = 0.27; P < .05). 3-Metoxytyramine was correlated with urea (r = 0.43; P < .01), creatinine (r = 0.32; P < .01), and the eGFR (r = -0.24; P < .05). Creatinine was correlated with age (r = 0.36; P < .01), diastolic blood pressure (r = 0.26; P < .05), time after heart transplantation (r = 0.24; P < .05), and renalase (r = 0.69; P < .01). Systolic blood pressure was correlated with proteinuria (r = 0.26; P < .05).
  
  
  CONCLUSIONS
  Chronic kidney disease and concomitant hypertension are the most prevalent comorbidities in the population of heart transplant recipients. Urine catecholamine metabolites were related to kidney function but not to blood pressure level in the studied population.
authors: Department of Cardiovascular Surgery and Transplantology, Jagiellonian University, Medical College, John Paul II Hospital, Cracow, Poland. Electronic address: grzegorz.wa@interia.pl.
authors: G
authors: G
authors: Wasilewski
authors: First Chair of General Surgery, Jagiellonian University, Medical College, Krakow, Poland. Silesian Center for Heart Diseases, Zabrze, Poland.
authors: P
authors: P
authors: Przybylowski
authors: Department of Clinical Biochemistry, Medical College, University Children's Hospital of Cracow, Jagiellonian University, Cracow, Poland.
authors: M
authors: M
authors: Wilusz
authors: Department of Clinical Biochemistry, Medical College, University Children's Hospital of Cracow, Jagiellonian University, Cracow, Poland.
authors: K
authors: K
authors: Sztefko
authors: Department of Cardiovascular Surgery and Transplantology, Jagiellonian University, Medical College, John Paul II Hospital, Cracow, Poland.
authors: Ł
authors: Ł
authors: Janik
authors: Second Department of Nephrology, Medical University of Bialystok, Poland.
authors: E
authors: E
authors: Koc-Żórawska
authors: Second Department of Nephrology, Medical University of Bialystok, Poland.
authors: J
authors: J
authors: Malyszko
mesh_terms: D000328:Adult; D000368:Aged; D001794:Blood Pressure / Q000502:physiology; D001795:Blood Pressure Determination; D018660:Blood Pressure Monitoring, Ambulatory; D002395:Catecholamines / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003404:Creatinine / Q000378:metabolism; D005260:Female; D005919:Glomerular Filtration Rate; D016027:Heart Transplantation; D006801:Humans; D006973:Hypertension / Q000209:etiology* / Q000652:urine; D007677:Kidney Function Tests; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D008995:Monoamine Oxidase / Q000378:metabolism / Q000502:physiology*; D051436:Renal Insufficiency, Chronic / Q000209:etiology* / Q000503:physiopathology / Q000652:urine
chemical_list: D002395:Catecholamines; D003404:Creatinine; D008995:Monoamine Oxidase; C521557:renalase