title: Combination of chlorogenic acid and salvianolic acid B protects against polychlorinated biphenyls-induced oxidative stress through Nrf2.
abstract: Caffeic acid derivatives (CADs) are well-known phytochemicals with multiple physiological and pharmacological activities. This study aimed to investigate the combined protective effects of CADs on PCB126-induced liver damages and oxidative stress in mice. Here, we used chemiluminescence and chose chlorogenic acid (CGA), salvianolic acid B (Sal B) as the best antioxidants. Then, mice were intragastrically administered with 60mg/kg/d CGA, Sal B, and CGA plus Sal B (1:1) for 3 weeks before exposing to 0.05mg/kg/d PCB126 for 2 weeks. We found that pretreatment with CGA, Sal B, and CGA plus Sal B effectively attenuated liver injury and cytotoxicity caused by PCB126, but improved the expressions of superoxide dismutase (SOD), glutathione reduced (GSH), heme oxygenase-1 (HO-1) and nuclear factor E2-related factor 2 (Nrf2), CGA plus Sal B especially, was found to have the best effects that indicated a synergetic protective effect. Taken together, as the Nrf2 regulates the cyto-protective response by up-regulating the expression of antioxidant genes, we suggested that CGA plus Sal B had a combined protection on PCB126-induced tissue damages and that the Nrf2 signaling might be involved.
authors: Department of Hygiene Analysis and Detection, School of Public Health, Nanjing Medical University, Nanjing 211166, People's Republic of China.
authors: Lijun
authors: L
authors: Chen
authors: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing 211166, People's Republic of China.
authors: Yuan
authors: Y
authors: Li
authors: Department of Hygiene Analysis and Detection, School of Public Health, Nanjing Medical University, Nanjing 211166, People's Republic of China.
authors: Wenqin
authors: W
authors: Yin
authors: Department of Hygiene Analysis and Detection, School of Public Health, Nanjing Medical University, Nanjing 211166, People's Republic of China.
authors: Wenqi
authors: W
authors: Shan
authors: The Key Laboratory of Modern Toxicology, Ministry of Education, Nanjing Medical University, Nanjing 211166, People's Republic of China.
authors: Jinfeng
authors: J
authors: Dai
authors: The Key Laboratory of Modern Toxicology, Ministry of Education, Nanjing Medical University, Nanjing 211166, People's Republic of China.
authors: Ye
authors: Y
authors: Yang
authors: Department of Hygiene Analysis and Detection, School of Public Health, Nanjing Medical University, Nanjing 211166, People's Republic of China. Electronic address: drleili@hotmail.com.
authors: Lei
authors: L
authors: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001572:Benzofurans / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002726:Chlorogenic Acid / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D004357:Drug Synergism; D004785:Environmental Pollutants / Q000633:toxicity*; D051547:Heme Oxygenase-1 / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000097:blood; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D053630:Depsides
chemical_list: D000975:Antioxidants; D001572:Benzofurans; D015415:Biomarkers; D002726:Chlorogenic Acid; D004785:Environmental Pollutants; D051547:Heme Oxygenase-1; D008565:Membrane Proteins; D011078:Polychlorinated Biphenyls; C076944:salvianolic acid B; D053630:Depsides; C496542:Hmox1 protein, mouse; C023035:3,4,5,3',4'-pentachlorobiphenyl
keywords: Chlorogenic acid; Nuclear factor E2-related factor 2; Oxidative stress; Salvianolic acid B; Synergetic effect