title: Identification of novel miRNAs from drought tolerant rice variety Nagina 22.
abstract: MicroRNAs regulate a spectrum of developmental and biochemical processes in plants and animals. Thus, knowledge of the entire miRNome is essential to understand the complete regulatory schema of any organism. The current study attempts to unravel yet undiscovered miRNA genes in rice. Analysis of small RNA libraries from various tissues of drought-tolerant 'aus' rice variety Nagina 22 (N22) identified 71 novel miRNAs. These were validated based on precursor hairpin structure, small RNA mapping pattern, 'star' sequence, conservation and identification of targets based on degradome data. While some novel miRNAs were conserved in other monocots and dicots, most appear to be lineage-specific. They were segregated into two different classes based on the closeness to the classical miRNA definition. Interestingly, evidence of a miRNA-like cleavage was found even for miRNAs that lie beyond the classical definition. Several novel miRNAs displayed tissue-enriched and/or drought responsive expression. Generation and analysis of the degradome data from N22 along with publicly available degradome identified several high confidence targets implicated in regulation of fundamental processes such as flowering and stress response. Thus, discovery of these novel miRNAs considerably expands the dimension of the miRNA-mediated regulation in rice.
authors: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi - 110021, India.
authors: Roseeta Devi
authors: RD
authors: Mutum
authors: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi - 110021, India.
authors: Santosh
authors: S
authors: Kumar
authors: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi - 110021, India.
authors: Sonia
authors: S
authors: Balyan
authors: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi - 110021, India.
authors: Shivani
authors: S
authors: Kansal
authors: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi - 110067, India.
authors: Saloni
authors: S
authors: Mathur
authors: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi - 110021, India.
authors: Saurabh
authors: S
authors: Raghuvanshi
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D055864:Droughts; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D012275:Oryza / Q000235:genetics / Q000502:physiology*; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics; D013312:Stress, Physiological
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant