title: Gene expression of MAGE-A3 and PRAME tumor antigens and EGFR mutational status in Taiwanese non-small cell lung cancer patients.
abstract: OBJECTIVE
  To determine the frequency of expression of the tumor-associated antigens (TAAs) melanoma-associated antigen A3 (MAGE-A3) and preferentially expressed antigen of melanoma (PRAME) and the rate of EGFR mutations in a Taiwanese non-small cell lung cancer (NSCLC) population including only adenocarcinomas and squamous cell carcinomas. Furthermore, to investigate associations between TAA expression and EGFR mutations and to evaluate these TAAs as prognostic markers for overall survival. The occurrence of single nucleotide polymorphisms in MAGEA3 and PRAME was also assessed.
  
  
  METHODS
  Archival fresh-frozen tumor tissue specimens were tested by quantitative reverse transcription polymerase chain reaction assays to detect MAGE-A3 and PRAME expression. EGFR mutations were detected by mass spectroscopy and single nucleotide polymorphisms by gene sequencing.
  
  
  RESULTS
  Of the 156 adenocarcinomas examined, 3.3% expressed MAGE-A3, 32.2% expressed PRAME and 62.8% had EGFR mutations. Of the 128 squamous cell carcinomas, 29.8% expressed MAGE-A3, 59.2% expressed PRAME and 20.5% harbored EGFR mutations. TAA expression was similar across subgroups determined by patient or tumor characteristics. There was no association between TAA expression and EGFR mutation status and TAA expression was found not to be a prognostic marker for survival. Single nucleotide polymorphisms were identified, one of which with a possible impact on MAGE-A3 expression.
  
  
  CONCLUSIONS
  In this NSCLC population, expression of MAGE-A3 and PRAME was more frequent in squamous cell carcinomas than in adenocarcinomas tumors. EGFR mutations were not associated with TAA expression for either histology and were three times more frequent in adenocarcinomas than in squamous cell carcinomas tumors.
authors: Graduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan.|Genome and Systems Biology Program, National Taiwan University and Academia Sinica, Taipei, Taiwan.|Ph.D. Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei, Taiwan.
authors: Szu-Hua
authors: SH
authors: Pan
authors: Center for Genomic Medicine, National Taiwan University, Taipei, Taiwan.|Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan.|Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan.
authors: Kang-Yi
authors: KY
authors: Su
authors: GSK Vaccines, Rixensart, Belgium.
authors: Bart
authors: B
authors: Spiessens
authors: GSK Vaccines, Rixensart, Belgium.
authors: Nicole
authors: N
authors: Kusuma
authors: GSK Vaccines, Rixensart, Belgium.
authors: Nicolas F
authors: NF
authors: Delahaye
authors: GSK Vaccines, Rixensart, Belgium.
authors: Olivier
authors: O
authors: Gruselle
authors: GSK Vaccines, Rixensart, Belgium.
authors: Aung
authors: A
authors: Myo
authors: GSK Vaccines, Rixensart, Belgium.
authors: An
authors: A
authors: de Creus
authors: GSK Vaccines, Rixensart, Belgium.
authors: Jamila
authors: J
authors: Louahed
authors: Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan.|Division of Chest Medicine, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan.|Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors: Gee-Cheng
authors: GC
authors: Chang
authors: Center for Genomic Medicine, National Taiwan University, Taipei, Taiwan.|Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan.|Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan.
authors: Sung-Liang
authors: SL
authors: Yu
authors: Center for Genomic Medicine, National Taiwan University, Taipei, Taiwan.|Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.|Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
authors: Pan-Chyr
authors: PC
authors: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009363:Neoplasm Proteins / Q000378:metabolism*; D011110:Polymorphism, Genetic; D012189:Retrospective Studies; D013624:Taiwan
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C072013:MAGEA3 protein, human; D009363:Neoplasm Proteins; C404944:PRAME protein, human
keywords: EGFR mutation; MAGE-A3 antigen; PRAME antigen; adenocarcinoma; non-small cell lung cancer; squamous cell carcinoma