title: Preliminary study of urine metabolism in type two diabetic patients based on GC-MS.
abstract: OBJECTIVE
  Comparative study of type 2 diabetes and healthy controls by metabolomics methods to explore the pathogenesis of Type II diabetes.
  
  
  METHODS
  Gas chromatography - mass spectrometry (GC-MS) with a variety of multivariate statistical analysis methods to the healthy control group 58 cases, 68 cases of Type II diabetes group were analyzed. Chromatographic conditions: DB-5MS column; the carrier gas He; flow rate of 1 mL·min(-1), the injection volume 1 uL; split ratio is 100: 1. MS conditions: electron impact (EI) ion source, an auxiliary temperature of 280°C, the ion source 230°C, quadrupole 150°C; mass scan range 30~600 mAu.
  
  
  RESULTS
  Established analytical method based on urine metabolomics GC-MS of Type II diabetes, determine the urine succinic acid, L-leucine, L-isoleucine, tyrosine, slanine, acetoace acid, mannose, L-isoleucine, L-threonine, Phenylalanine, fructose, D-glucose, palmi acid, oleic acid and arachidonic acid were significantly were significantly changed.
  
  
  CONCLUSIONS
  Based on metabolomics of GC-MS detection and analysis metabolites can be found differences between type 2 diabetes and healthy control group, PCA diagram can effectively distinguish Type II diabetes and healthy control group, with load diagrams and PLS-DA VIP value metabolite screening, the resulting differences in metabolic pathways involved metabolites, including amino acid metabolism, lipid metabolism, glucose metabolism and energy metabolism.
authors: College of Jiamusi, Heilongjiang University of Chinese Medicine Jiamusi, Heilongjiang 154007, P. R. China.
authors: Ning
authors: N
authors: Zhang
authors: College of Chemistry & Chemical Engineering, Harbin Normal University Harbin, Heilongjiang 150025, P. R. China.
authors: Fang
authors: F
authors: Geng
authors: College of Pharmacy, Heilongjiang University of Chinese Medicine Harbin, Heilongjiang 150040, P. R. China.
authors: Zhong-Hua
authors: ZH
authors: Hu
authors: College of Jiamusi, Heilongjiang University of Chinese Medicine Jiamusi, Heilongjiang 154007, P. R. China.
authors: Bin
authors: B
authors: Liu
authors: College of Jiamusi, Heilongjiang University of Chinese Medicine Jiamusi, Heilongjiang 154007, P. R. China.
authors: Ye-Qiu
authors: YQ
authors: Wang
authors: College of Pharmacy, Heilongjiang University of Chinese Medicine Harbin, Heilongjiang 150040, P. R. China.
authors: Jun-Cen
authors: JC
authors: Liu
authors: College of Jiamusi, Heilongjiang University of Chinese Medicine Jiamusi, Heilongjiang 154007, P. R. China.
authors: Yong-Hua
authors: YH
authors: Qi
authors: College of Pharmacy, Changchun University of Chinese Medicine Changchun, Jilin 130117, P. R. China.
authors: Li-Jing
authors: LJ
authors: Li
keywords: Type 2 diabetes mellitu; gas chromatography-mass spectrometry (GC-MS); metabolomics; urine