title: Validation of antibodies for neuroanatomical localization of the P2Y11receptor in macaque brain.
abstract: Focus on the purinergic receptor P2Y11has increased following the finding of an association between the sleep disorder narcolepsy and a genetic variant in P2RY11 causing decreased gene expression. Narcolepsy is believed to arise from an autoimmune destruction of the hypothalamic neurons that produce the neuropeptide hypocretin/orexin. It is unknown how a decrease in expression of P2Y11might contribute to an autoimmune reaction towards the hypocretin neurons and the development of narcolepsy. To advance narcolepsy research it is therefore extremely important to determine the neuroanatomical localization of P2Y11in the brain with particular emphasis on the hypocretin neurons. In this article we used western blot, staining of blood smears, and flow cytometry to select two antibodies for immunohistochemical staining of macaque monkey brain. Staining was seen in neuron-like structures in cortical and hypothalamic regions. Rats do not have a gene orthologue to the P2Y11receptor and therefore rat brain was used as negative control tissue. The chromogenic signal observed in macaque monkey brain in neurons was not considered reliable, because the antibodies stained rat brain in a similar distribution pattern. Hence, the neuroanatomical localization of the P2Y11receptor remains undetermined due to the lack of specific P2Y11antibodies for brain immunohistochemistry.
authors: Molecular Sleep Lab, Department of Clinical Biochemistry, Rigshospitalet, Glostrup, Denmark.
authors: Karin
authors: K
authors: Dreisig
authors: Molecular Sleep Lab, Department of Clinical Biochemistry, Rigshospitalet, Glostrup, Denmark.
authors: Matilda
authors: M
authors: Degn
authors: Molecular Sleep Lab, Department of Clinical Biochemistry, Rigshospitalet, Glostrup, Denmark.
authors: Louise
authors: L
authors: Sund
authors: Department of Neurological Surgery, University of California, San Francisco, USA.
authors: Piotr
authors: P
authors: Hadaczek
authors: Department of Neurological Surgery, University of California, San Francisco, USA.
authors: Lluis
authors: L
authors: Samaranch
authors: Department of Neurological Surgery, University of California, San Francisco, USA.
authors: Waldy
authors: W
authors: San Sebastian
authors: Department of Neurological Surgery, University of California, San Francisco, USA.
authors: Krystof
authors: K
authors: Bankiewicz
authors: Molecular Sleep Lab, Department of Clinical Biochemistry, Rigshospitalet, Glostrup, Denmark; Danish Center for Sleep Medicine, Department of Clinical Neurophysiology, Rigshospitalet, Glostrup, Denmark. Electronic address: birgitte.kornum@regionh.dk.
authors: Birgitte
authors: B
authors: Rahbek Kornum
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D008251:Macaca; D009474:Neurons / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000378:metabolism*; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000378:metabolism*
chemical_list: D018048:Receptors, Purinergic P2
keywords: Lymphocyte; P2RY11; P2Y(1); P2Y(2); Primate; Rat