title: Structure and bioactivity of a polysaccharide containing uronic acid from Polyporus umbellatus sclerotia.
abstract: Polyporus umbellatus is a medicinal fungus, has been used in traditional Chinese medicine for thousands years for treatment of edema, scanty urine, vaginal discharge, jaundice and diarrhea. The structure of a soluble polysaccharide (named PUP80S1), purified from the sclerotia of Polyporus umbellatus was elucidated by gas chromatography (GC), GC-mass spectrometry and nuclear magnetic resonance spectroscopy. PUP80S1 is a branched polysaccharide containing approximately 8.5% uronic acid and having an average molecular weight of 8.8kDa. Atomic force microscopy of PUP80S1 reveals a globular chain conformation in water. Antioxidant tests, Oxygen radical absorption capacity and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays indicate that PUP80S1 possesses significant antioxidant activity. But the related polysaccharide, PUP60S2, which contains more uronic acid residues and a higher level of branching, shows better antioxidant activity. These results suggest that structure features of polysaccharides play an important role in their physiological functions.
authors: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China; College of Ocean, Zhejiang University of Technology, Hangzhou 310032, China.
authors: Pengfei
authors: P
authors: He
authors: College of Ocean, Zhejiang University of Technology, Hangzhou 310032, China; Departments of Chemistry and Chemical Biology and Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA. Electronic address: zhanganqiang@zjut.edu.cn.
authors: Anqiang
authors: A
authors: Zhang
authors: Departments of Chemistry and Chemical Biology and Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors: Fuming
authors: F
authors: Zhang
authors: Departments of Chemistry and Chemical Biology and Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA; Department of Biological Science, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors: Robert J
authors: RJ
authors: Linhardt
authors: College of Ocean, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: sun_pl@zjut.edu.cn.
authors: Peilong
authors: P
authors: Sun
mesh_terms: D000975:Antioxidants / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D004040:Dietary Carbohydrates / Q000138:chemical synthesis / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D000072257:Oxygen Radical Absorbance Capacity; D010851:Picrates / Q000737:chemistry; D055492:Polyporus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D014574:Uronic Acids / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D004040:Dietary Carbohydrates; D010851:Picrates; D011134:Polysaccharides; D014574:Uronic Acids; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant activity; Atomic force microscopy; Nuclear magnetic resonance spectroscopy; Polyporus umbellatus; Polysaccharide; Structure-function relationship