title: 16S rRNA amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae.
abstract: Data presented here contains metagenomic analysis regarding the sequential conventionalization of germ-free zebrafish embryos. Zebrafish embryos that underwent a germ-free sterilization process immediately after fertilization were promptly exposed to and raised to larval stage in conventional fish water. At 6 days postfertilization (dpf), these "conventionalized" larvae were compared to zebrafish larvae that were raised in conventional fish water never undergoing the initial sterilization process. Bacterial 16S rRNA amplicon sequencing was performed on DNA isolated from homogenates of the larvae revealing distinct microbiota variations between the two groups. The dataset described here is also related to the research article entitled "Microbial modulation of behavior and stress responses in zebrafish larvae" (Davis et al., 2016) [1].
authors: Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65201, USA.
authors: Daniel J
authors: DJ
authors: Davis
authors: Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65201, USA.
authors: Elizabeth C
authors: EC
authors: Bryda
authors: Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65201, USA.
authors: Catherine H
authors: CH
authors: Gillespie
authors: Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65201, USA; University of Missouri Metagenomics Center (MUMC), University of Missouri, Columbia, MO 65201, USA.
authors: Aaron C
authors: AC
authors: Ericsson
keywords: 16S rRNA sequencing; Gnotobiotic; Microbiome; Microbiota; Zebrafish larvae