title: Fibrosis-Inducing Strategies in Regenerating Dystrophic and Normal Skeletal Muscle.
abstract: The excessive accumulation of collagens (fibrosis) impairs the function of vital tissues and organs. Fibrosis is a hallmark of severe muscular dystrophies, such as the incurable Duchenne Muscular Dystrophy (DMD), where skeletal muscle is substituted by scar (fibrotic) tissue as disease advances. One of the major obstacles in increasing our ability to combat fibrosis-driven muscular dystrophy progression is that no optimal in vivo models of muscle fibrosis are currently available, limiting fibrosis research and the development of novel therapies. In this chapter we describe different experimental strategies to accelerate and enhance muscle fibrosis in vivo in the widely used animal model for DMD, the mdx mouse. Since excessive tissue scarring also hampers the normal regeneration process after muscle injury, we have extended these fibrogenic strategies to the muscle of normal (non-diseased) mice. These strategies will allow fibrosis induction and assessment in a wide array of genetically modified mouse lines in physiological and pathological conditions of muscle regeneration. They should eventually improve our ability to combat fibrosis and foster muscle regeneration in DMD.
authors: Cell Biology Group, Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), CIBER on Neurodegenerative Diseases (CIBERNED), Barcelona, Spain. patrizia.pessina@upf.edu.
authors: Patrizia
authors: P
authors: Pessina
authors: Cell Biology Group, Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), CIBER on Neurodegenerative Diseases (CIBERNED), Barcelona, Spain. pura.munoz@upf.edu.|Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain. pura.munoz@upf.edu.|Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain. pura.munoz@upf.edu.
authors: Pura
authors: P
authors: Muñoz-Cánoves
mesh_terms: D000818:Animals; D054715:Cardiotoxins / Q000009:adverse effects; D003435:Crotalid Venoms / Q000009:adverse effects; D004195:Disease Models, Animal; D005355:Fibrosis; D007150:Immunohistochemistry; D051379:Mice; D018101:Mice, Inbred mdx; D053580:Muscle Strength; D018482:Muscle, Skeletal / Q000187:drug effects / Q000473:pathology* / Q000502:physiology*; D020388:Muscular Dystrophy, Duchenne / Q000473:pathology*; D012038:Regeneration
chemical_list: D054715:Cardiotoxins; D003435:Crotalid Venoms
keywords: Chronic injury; Duchenne muscular dystrophy; Fibrosis; Skeletal muscle regeneration; mdx mice