title: Ovicidal and larvicidal activity of extracts of Opuntia ficus-indica against gastrointestinal nematodes of naturally infected sheep.
abstract: This study describes the in vitro anthelmintic activity of extracts from Opuntia ficus indica against gastrointestinal nematodes of sheep. The anthelmintic activity was evaluated by inhibition of egg hatching, larval development and larval migration assays. The residual aqueous fractions from cladodes and fruits showed higher ovicidal activity with EC50 values of 7.2mg/mL and 1.5mg/mL, respectively. The aqueous, hexane, and ethyl acetate fractions from fruits and the aqueous fraction from cladodes inhibited 100% of larval development at the lowest concentration tested (1.56mg/mL). The crude cladode and fruit ethanolic extracts inhibited larval migration and showed EC50 values of 0.74mg/mL and 0.27mg/mL, respectively. Phytochemical screening detected high concentrations of alkaloids, tannins, flavonoids, and saponins in the fruits and cladodes. The results demonstrated that O. ficus exhibits anthelmintic activity in vitro, suggesting that, beyond its nutritional potential, this plant can also be an ally for parasite control in sheep.
authors: Departamento de Física e Química, FEIS, Universidade Estadual Paulista, CEP 15385-000, SP, Brazil.
authors: Aline
authors: A
authors: Féboli
authors: Departamento de Biologia e Zootecnia, FEIS, Universidade Estadual Paulista, CEP 15385-000, SP, Brazil.
authors: Antonio C
authors: AC
authors: Laurentiz
authors: Departamento de Biologia e Zootecnia, FEIS, Universidade Estadual Paulista, CEP 15385-000, SP, Brazil.
authors: Suelen C S
authors: SC
authors: Soares
authors: Departamento de Biologia e Zootecnia, FEIS, Universidade Estadual Paulista, CEP 15385-000, SP, Brazil.
authors: Jeferson G
authors: JG
authors: Augusto
authors: Departamento de Biologia e Zootecnia, FEIS, Universidade Estadual Paulista, CEP 15385-000, SP, Brazil.
authors: Luciano A
authors: LA
authors: Anjos
authors: Núcleo de Pesquisa em Ciências Exatas e Tecnológicas, Universidade de Franca, CEP 14404-600, SP, Brazil.
authors: Lizandra G
authors: LG
authors: Magalhães
authors: Departamento de Biologia e Zootecnia, FEIS, Universidade Estadual Paulista, CEP 15385-000, SP, Brazil.
authors: Rosemeire S
authors: RS
authors: Filardi
authors: Departamento de Física e Química, FEIS, Universidade Estadual Paulista, CEP 15385-000, SP, Brazil. Electronic address: rosangela@dfq.feis.unesp.br.
authors: Rosangela S
authors: RS
authors: Laurentiz
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000302:isolation & purification / Q000494:pharmacology*; D006189:Haemonchus / Q000187:drug effects / Q000254:growth & development; D020128:Inhibitory Concentration 50; D007411:Intestinal Diseases, Parasitic / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary; D007814:Larva / Q000187:drug effects / Q000502:physiology; D009349:Nematode Infections / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D029747:Opuntia / Q000737:chemistry*; D010063:Ovum / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy / Q000469:parasitology*; D013634:Tannins / Q000302:isolation & purification / Q000494:pharmacology; D014254:Trichostrongylus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts; D012503:Saponins; D013634:Tannins
keywords: Anthelmintic activity; Cactus forage; Phytotherapy