title: Remote ischemic preconditioning improves post resuscitation cerebral function via overexpressing neuroglobin after cardiac arrest in rats.
abstract: In this study, we investigated the effects of remote ischemic preconditioning on post resuscitation cerebral function in a rat model of cardiac arrest and resuscitation. The animals were randomized into six groups: 1) sham operation, 2) lateral ventricle injection and sham operation, 3) cardiac arrest induced by ventricular fibrillation, 4) lateral ventricle injection and cardiac arrest, 5) remote ischemic preconditioning initiated 90min before induction of ventricular fibrillation, and 6) lateral ventricle injection and remote ischemic preconditioning before cardiac arrest. Reagent of Lateral ventricle injection is neuroglobin antisense oligodeoxynucleotides which initiated 24h before sham operation, cardiac arrest or remote ischemic preconditioning. Remote ischemic preconditioning was induced by four cycles of 5min of limb ischemia, followed by 5min of reperfusion. Ventricular fibrillation was induced by current and lasted for 6min. Defibrillation was attempted after 6min of cardiopulmonary resuscitation. The animals were then monitored for 2h and observed for an additionally maximum 70h. Post resuscitation cerebral function was evaluated by neurologic deficit score at 72h after return of spontaneous circulation. Results showed that remote ischemic preconditioning increased neurologic deficit scores. To investigate the neuroprotective effects of remote ischemic preconditioning, we observed neuronal injury at 48 and 72h after return of spontaneous circulation and found that remote ischemic preconditioning significantly decreased the occurrence of neuronal apoptosis and necrosis. To further comprehend mechanism of neuroprotection induced by remote ischemic preconditioning, we found expression of neuroglobin at 24h after return of spontaneous circulation was enhanced. Furthermore, administration of neuroglobin antisense oligodeoxynucleotides before induction of remote ischemic preconditioning showed that the level of neuroglobin was decreased then partly abrogated neuroprotection of remote ischemic preconditioning. These date suggested that neuroglobin involved in neuroprotective effect of remote ischemic preconditioning. In conclusion, remote ischemic preconditioning attenuated post resuscitation cerebral dysfunction and the neuroprotection was mediated partly by high level of neuroglobin in a rat model of cardiac arrest and resuscitation.
authors: The First Affiliated Hospital of Sun Yet-sen University, Guang Zhou, Guang Dong, China; Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou, Guang Dong, China; Department of Emergency, the Zhong Shan Affiliated Hospital of Sun Yet-sen University, Zhong Shan, Guang Dong, China.
authors: Ran
authors: R
authors: Fan
authors: Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou, Guang Dong, China.
authors: Tao
authors: T
authors: Yu
authors: Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou, Guang Dong, China.
authors: Jia-Li
authors: JL
authors: Lin
authors: Department of Emergency, the Zhong Shan Affiliated Hospital of Sun Yet-sen University, Zhong Shan, Guang Dong, China.
authors: Guang-Dong
authors: GD
authors: Ren
authors: Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou, Guang Dong, China.
authors: Yi
authors: Y
authors: Li
authors: The First Affiliated Hospital of Sun Yet-sen University, Guang Zhou, Guang Dong, China.
authors: Xiao-Xing
authors: XX
authors: Liao
authors: Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou, Guang Dong, China.
authors: Zi-Tong
authors: ZT
authors: Huang
authors: Department of Emergency, the Zhong Shan Affiliated Hospital of Sun Yet-sen University, Zhong Shan, Guang Dong, China. Electronic address: DrCHJiang@163.com.
authors: Chong-Hui
authors: CH
authors: Jiang
mesh_terms: D000818:Animals; D001921:Brain / Q000503:physiopathology*; D056547:CA1 Region, Hippocampal / Q000378:metabolism / Q000473:pathology; D016887:Cardiopulmonary Resuscitation; D016923:Cell Death; D004195:Disease Models, Animal; D005914:Globins / Q000378:metabolism*; D006323:Heart Arrest / Q000150:complications / Q000517:prevention & control*; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D000078787:Neuroglobin; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D005914:Globins
keywords: Cardiopulmonary resuscitation; Cerebral function; Neuroglobin; Remote ischemic preconditioning