title: Electrospun Poly(l-lactide)/Poly(ethylene glycol) Scaffolds Seeded with Human Amniotic Mesenchymal Stem Cells for Urethral Epithelium Repair.
abstract: Tissue engineering-based urethral replacement holds potential for repairing large segmental urethral defects, which remains a great challenge at present. This study aims to explore the potential of combining biodegradable poly(l-lactide) (PLLA)/poly(ethylene glycol) (PEG) scaffolds and human amniotic mesenchymal cells (hAMSCs) for repairing urethral defects. PLLA/PEG fibrous scaffolds with various PEG fractions were fabricated via electrospinning. The scaffolds were then seeded with hAMSCs prior to implantation in New Zealand male rabbits that had 2.0 cm-long defects in the urethras. The rabbits were randomly divided into three groups. In group A, hAMSCs were grown on PLLA/PEG scaffolds for two days and then implanted to the urethral defects. In group B, only the PLLA/PEG scaffolds were used to rebuild the rabbit urethral defect. In group C, the urethral defect was reconstructed using a regular urethral reparation technique. The repair efficacy was compared among the three groups by examining the urethral morphology, tissue reconstruction, luminal patency, and complication incidence (including calculus formation, urinary fistula, and urethral stricture) using histological evaluation and urethral radiography methods. Findings from this study indicate that hAMSCs-loaded PLLA/PEG scaffolds resulted in the best urethral defect repair in rabbits, which predicts the promising application of a tissue engineering approach for urethral repair.
authors: Departments of Urology and Orthopaedic Surgery, The First Affiliated Hospital, Orthopaedic Institute, Soochow University, 708 Renmin Rd., Suzhou 215007, China. xiaokui86@163.com.
authors: Xiaokui
authors: X
authors: Lv
authors: Departments of Urology and Orthopaedic Surgery, The First Affiliated Hospital, Orthopaedic Institute, Soochow University, 708 Renmin Rd., Suzhou 215007, China. guoqianping@suda.edu.cn.
authors: Qianping
authors: Q
authors: Guo
authors: Departments of Urology and Orthopaedic Surgery, The First Affiliated Hospital, Orthopaedic Institute, Soochow University, 708 Renmin Rd., Suzhou 215007, China. fxhan@suda.edu.cn.
authors: Fengxuan
authors: F
authors: Han
authors: Departments of Urology and Orthopaedic Surgery, The First Affiliated Hospital, Orthopaedic Institute, Soochow University, 708 Renmin Rd., Suzhou 215007, China. 18862238857@163.com.
authors: Chunyang
authors: C
authors: Chen
authors: Departments of Urology and Orthopaedic Surgery, The First Affiliated Hospital, Orthopaedic Institute, Soochow University, 708 Renmin Rd., Suzhou 215007, China. christopherwfling@gmail.com.
authors: Christopher
authors: C
authors: Ling
authors: Departments of Urology and Orthopaedic Surgery, The First Affiliated Hospital, Orthopaedic Institute, Soochow University, 708 Renmin Rd., Suzhou 215007, China. 15312172967@163.com.
authors: Weiguo
authors: W
authors: Chen
authors: Departments of Urology and Orthopaedic Surgery, The First Affiliated Hospital, Orthopaedic Institute, Soochow University, 708 Renmin Rd., Suzhou 215007, China. binli@suda.edu.cn.
authors: Bin
authors: B
authors: Li
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D007773:Lactates / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D007773:Lactates; D011091:Polyesters; C088513:poly(lactic acid-ethylene glycol); D011092:Polyethylene Glycols; C033616:poly(lactide)
keywords: ">l-lactide); electrospinning; human amniotic mesenchymal cells; poly(; poly(ethylene glycol); tissue engineering; urethral defect