title: Neuroprotective effects of Kaempferide-7-O-(4″-O-acetylrhamnosyl)-3-O-rutinoside on cerebral ischemia-reperfusion injury in rats.
abstract: In the present study, we aim to evaluate the potential neuroprotective effect and the underlying mechanism of Kaempferide-7-O-(4″-O-acetylrhamnosyl)-3-O-rutinoside (A-F-B) against cerebral I/R injury. Adult male rats were pretreated with A-F-B by intragastric administration once a day for 3 days. One hour after the third day administration, animals were subjected to 2h of transient middle cerebral artery occlusion (MCAO) followed by 24h of reperfusion. Neurological deficit, infarct volume, histopathological changes, oxidative stress-related biochemical parameters, neuronal apoptosis, apoptosis-related proteins and the expression of pro-inflammator cytokines genes were measured. A-F-B significantly decreased neurological and histological deficits, reduced the infarct volume, and decreased neuroapoptosis. Meanwhile, A-F-B inhibited the expression of Bax, cleaved caspase-3, cleaved caspase-9, and promoted Bcl-2 expression. In addition, the expression of pro-inflammator cytokines, including phospho-NF-kBp65, interleukin-1β, interleukin-6, tumor necrosis factor-α, intercellular adhesion molecule-1, cyclooxygenase-2 and inducible nitric oxide synthase, were also suppressed by A-F-B pretreatment. Furthermore, pretreatment with A-F-B could significantly increase the activities of superoxide dismutase, glutathione peroxidase, but decrease the content of malondiadehyde in blood serum. These results suggest that A-F-B has the neuroprotective effect in ischemic stroke by suppressing neuroinflammation, reactive oxygen species and neuroapoptosis.
authors: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun, PR China.
authors: Shuaijun
authors: S
authors: Wang
authors: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun, PR China.
authors: Huali
authors: H
authors: Xu
authors: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun, PR China.
authors: Ying
authors: Y
authors: Xin
authors: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun, PR China.
authors: Maowei
authors: M
authors: Li
authors: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun, PR China.
authors: Wenwen
authors: W
authors: Fu
authors: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun, PR China.
authors: Yuchen
authors: Y
authors: Wang
authors: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun, PR China.
authors: Zeyuan
authors: Z
authors: Lu
authors: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun, PR China.
authors: Xiaofeng
authors: X
authors: Yu
authors: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun, PR China. Electronic address: suidy@jlu.edu.cn.
authors: Dayun
authors: D
authors: Sui
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D020520:Brain Infarction / Q000150:complications*; D005786:Gene Expression Regulation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D006027:Glycosides / Q000494:pharmacology* / Q000627:therapeutic use; D018836:Inflammation Mediators / Q000378:metabolism; D044949:Kaempferols / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018696:Neuroprotective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D006027:Glycosides; D018836:Inflammation Mediators; D044949:Kaempferols; D018696:Neuroprotective Agents; C571783:kaempferide-7-O-(4''-O-acetylrhamnosyl)-3-O-rutinoside; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Apoptosis; Cerebral ischemia; Kaempferide-7-O-(4″-O-acetylrhamnosyl)-3-O-rutinoside; Oxidative stress; Reperfusion