title: Production of trans-10,cis-12-conjugated linoleic acid using permeabilized whole-cell biocatalyst of Yarrowia lipolytica.
abstract: OBJECTIVE
  To improve the production of trans-10,cis-12-conjugated linoleic acid (t10,c12-CLA) from linoleic acid in recombinant Yarrowia lipolytica.
  
  
  RESULTS
  Cells of the yeast were permeabilized by freeze/thawing. The optimal conditions for t10,c12-CLA production by the permeabilized cells were at 28 °C, pH 7, 200 rpm with 1.5 g sodium acetate l-1, 100 g wet cells l-1, and 25 g LA l-1. Under these conditions, the permeabilized cells produced 15.6 g t10,c12-CLA l-1after 40 h, with a conversion yield of 62 %. The permeabilized cells could be used repeatedly for three cycles, with the t10,c12-CLA extracellular production remaining above 10 g l-1.
  
  
  CONCLUSIONS
  Synthesis of t10,c12-CLA was achieved using a novel method, and the production reported in this work is the highest value reported to date.
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors: Baixi
authors: B
authors: Zhang
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors: Yuhang
authors: Y
authors: Song
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors: Haiqin
authors: H
authors: Chen
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors: Hao
authors: H
authors: Zhang
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China. chenwei66@jiangnan.edu.cn.|Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology & Business University, Beijing, 100048, People's Republic of China. chenwei66@jiangnan.edu.cn.
authors: Wei
authors: W
authors: Chen
mesh_terms: D001431:Bacteriological Techniques; D055162:Biocatalysis; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis*; D025062:Yarrowia / Q000378:metabolism*
chemical_list: D044243:Linoleic Acids, Conjugated; C496197:trans-10,cis-12-conjugated linoleic acid
keywords: Linoleic acid; Permeabilizated cells; Reuse; Whole-cell catalysis; Yarrowia lipolytica; trans-10,cis-12-conjugated linoleic acid