title: Effect of Fluoxetine on Neurogenesis in Hippocampal Dentate Gyrus after Global Transient Cerebral Ischemia in Rats.
abstract: Changes in cerebral neurogenesis provoked by ischemia and the effect of fluoxetine on this process were studied using a three-vessel occlusion model of global transient cerebral ischemia. The global transient cerebral ischemia was modeled on male Wistar rats by transient occlusion of three major vessels originating from the aortic arch and supplying the brain (brachiocephalic trunk, left subclavian artery, and left common carotid artery). The cells expressing doublecortin (DCX, a marker of young neurons) were counted in the hippocampal dentate gyrus on day 31 after ischemia modeling. It was found that ischemia inhibited neurogenesis in the dentate gyrus in comparison with sham-operated controls (p<0.05), while fluoxetine (20 mg/kg/day) injected over 10 days after surgery restored neurogenesis to the control level (p<0.001).
authors: Department of Neurobiology, Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, Russia. khodanovich@mail.tsu.ru.
authors: M Yu
authors: MY
authors: Khodanovich
authors: Department of Neurobiology, Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, Russia.
authors: A A
authors: AA
authors: Kisel'
authors: Department of Circulation Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors: G A
authors: GA
authors: Chernysheva
authors: Department of Circulation Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors: V I
authors: VI
authors: Smol'yakova
authors: Department of Neurobiology, Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, Russia.
authors: R R
authors: RR
authors: Savchenko
authors: Department of Circulation Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors: M B
authors: MB
authors: Plotnikov
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000166:cytology* / Q000187:drug effects*; D000089662:Doublecortin Protein; D005473:Fluoxetine / Q000627:therapeutic use*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects; D002546:Ischemic Attack, Transient / Q000188:drug therapy*; D008297:Male; D055495:Neurogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D005473:Fluoxetine
keywords: dentate gyrus; fluoxetine; global transient cerebral ischemia; hippocampus; neurogenesis