title: Non-invasive detection of hepatocellular carcinoma serum metabolic profile through surface-enhanced Raman spectroscopy.
abstract: The present study aims to identify distinctive Raman spectrum metabolic peaks to predict hepatocellular carcinoma (HCC). We performed a label-free, non-invasive surface-enhanced Raman spectroscopy (SERS) test on 230 serum samples including 47 HCC, 60 normal controls (NC), 68 breast cancer (BC) and 55 lung cancer (LC) by mixing Au@AgNRs with serum directly. Based on the observed SERS spectra, discriminative metabolites including tryptophan, phenylalanine, and etc. were found in HCC, when compared with BC, LC, and NC (P<0.05 in all). Common metabolites-proline, valine, adenine and thymine were found in HCC, BC and LC with compared to NC group (P<0.05). Importantly, Raman spectra of HCC serum biomarker AFP were firstly detected to analyze the HCC prominent peak. Orthogonal partial least squares discriminant analysis was adopted to assess the diagnostic accuracy; area under curve value of HCC is 0.991. This study provides new insights into the HCC metabolites detection through Raman spectroscopy.
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Rui
authors: R
authors: Xiao
authors: Department of Bio-diagnosis, Institute of Basic Medical Sciences, Beijing, China.
authors: Xuhui
authors: X
authors: Zhang
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Zhen
authors: Z
authors: Rong
authors: Department of Bio-diagnosis, Institute of Basic Medical Sciences, Beijing, China.
authors: Bingshui
authors: B
authors: Xiu
authors: Department of Bio-diagnosis, Institute of Basic Medical Sciences, Beijing, China.
authors: Xiqin
authors: X
authors: Yang
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Chongwen
authors: C
authors: Wang
authors: Affiliated 307 Hospital, Beijing, China.
authors: Wende
authors: W
authors: Hao
authors: National Center of Biomedical Analysis, Beijing, China.
authors: Qi
authors: Q
authors: Zhang
authors: Department of Bio-diagnosis, Institute of Basic Medical Sciences, Beijing, China.
authors: Zhiqiang
authors: Z
authors: Liu
authors: Department of Bio-diagnosis, Institute of Basic Medical Sciences, Beijing, China.
authors: Cuimi
authors: C
authors: Duan
authors: Department of Radiotherapy and Chemotherapy, Tangshan City People's Hospital, Tangshan, China.
authors: Kai
authors: K
authors: Zhao
authors: Clinical Laboratory, 252 Hospital of PLA, Baoding, China.
authors: Xu
authors: X
authors: Guo
authors: Department of Bio-diagnosis, Institute of Basic Medical Sciences, Beijing, China.
authors: Yawen
authors: Y
authors: Fan
authors: Department of Bio-diagnosis, Institute of Basic Medical Sciences, Beijing, China.
authors: Yanfeng
authors: Y
authors: Zhao
authors: Olympia Diagnostics, Inc. Sunnyvale, CA, USA.
authors: Heather
authors: H
authors: Johnson
authors: Affiliated 307 Hospital, Beijing, China.
authors: Yan
authors: Y
authors: Huang
authors: Department of Bio-diagnosis, Institute of Basic Medical Sciences, Beijing, China. Electronic address: xyfeng2002@126.com.
authors: Xiaoyan
authors: X
authors: Feng
authors: Zhejiang Cancer Hospital, Hangzhou, China. Electronic address: zjhzxxh@163.com.
authors: Xiaohong
authors: X
authors: Xu
authors: Department of Bio-diagnosis, Institute of Basic Medical Sciences, Beijing, China. Electronic address: zhangheqiu2004@126.com.
authors: Heqiu
authors: H
authors: Zhang
authors: Beijing Institute of Radiation Medicine, Beijing, China. Electronic address: sqwang@bmi.ac.cn.
authors: Shengqi
authors: S
authors: Wang
mesh_terms: D014408:Biomarkers, Tumor; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging*; D006801:Humans; D008113:Liver Neoplasms / Q000000981:diagnostic imaging*; D055442:Metabolome; D013059:Spectrum Analysis, Raman
chemical_list: D014408:Biomarkers, Tumor
keywords: Au@Ag nanorods; Cancer detection; Hepatocellular carcinoma; Serum metabolites; Surface-enhanced Raman scattering