title: Rapid flow cytometric measurement of protein inclusions and nuclear trafficking.
abstract: Proteinaceous cytoplasmic inclusions are an indicator of dysfunction in normal cellular proteostasis and a hallmark of many neurodegenerative diseases. We describe a simple and rapid new flow cytometry-based method to enumerate, characterise and, if desired, physically recover protein inclusions from cells. This technique can analyse and resolve a broad variety of inclusions differing in both size and protein composition, making it applicable to essentially any model of intracellular protein aggregation. The method also allows rapid quantification of the nuclear trafficking of fluorescently labelled molecules.
authors: Illawarra Health and Medical Research Institute and School of Biological Sciences, University of Wollongong, Wollongong NSW2522, Australia.
authors: D R
authors: DR
authors: Whiten
authors: Illawarra Health and Medical Research Institute and School of Biological Sciences, University of Wollongong, Wollongong NSW2522, Australia.
authors: R
authors: R
authors: San Gil
authors: Illawarra Health and Medical Research Institute and School of Biological Sciences, University of Wollongong, Wollongong NSW2522, Australia.
authors: L
authors: L
authors: McAlary
authors: Illawarra Health and Medical Research Institute and School of Biological Sciences, University of Wollongong, Wollongong NSW2522, Australia.
authors: J J
authors: JJ
authors: Yerbury
authors: Illawarra Health and Medical Research Institute and School of Biological Sciences, University of Wollongong, Wollongong NSW2522, Australia.
authors: H
authors: H
authors: Ecroyd
authors: Illawarra Health and Medical Research Institute and School of Biological Sciences, University of Wollongong, Wollongong NSW2522, Australia.
authors: M R
authors: MR
authors: Wilson
mesh_terms: D002138:Calibration; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism*; D019636:Neurodegenerative Diseases / Q000378:metabolism; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism*
chemical_list: D011506:Proteins