title: Use of panel testing for detection of antinuclear antibody in a resource-limited setting: an appraisal.
abstract: OBJECTIVE
  Despite an increase in the incidence of systemic connective tissue diseases (CTD), panel testing for detection of antinuclear antibodies (ANA) is not a routine practice in many health centers of the Indian subcontinent. Consequently, the data on its significance is scanty.
  
  
  METHODS
  To evaluate utility of panel testing, line immunoassay (LIA) and indirect immunofluorescence antinuclear antibody test (IIF-ANA) were performed in 321 cases of CTD.
  
  
  RESULTS
  Out of 321 serum samples screened by the above tests, 227 were positive and 18 were negative by both LIA and IIF-ANA. Additional 11/321 (3.4%) cases were picked up by LIA. SSA was most common specificity in these cases followed by SSA/SSB, SSB, Ro-52, Jo-1, dsDNA and nRNP/Sm.
  
  
  CONCLUSIONS
  Use of LIA along with IF-ANA and ELISA improves sensitivity of CTD screening.
authors: a Department of Immunopathology , Post Graduate Institute of Medical Education & Research , Chandigarh , India.
authors: Ranjana Walker
authors: RW
authors: Minz
authors: a Department of Immunopathology , Post Graduate Institute of Medical Education & Research , Chandigarh , India.
authors: Yashwant
authors: Y
authors: Kumar
authors: a Department of Immunopathology , Post Graduate Institute of Medical Education & Research , Chandigarh , India.
authors: Biman
authors: B
authors: Saikia
authors: a Department of Immunopathology , Post Graduate Institute of Medical Education & Research , Chandigarh , India.
authors: Shashi
authors: S
authors: Anand
authors: b Department of Internal Medicine , Post Graduate Institute of Medical Education & Research , Chandigarh , India.
authors: Subhash
authors: S
authors: Varma
authors: c Department of Rheumatology , Post Graduate Institute of Medical Education & Research , Chandigarh , India.
authors: Surjit
authors: S
authors: Singh
mesh_terms: D000974:Antibodies, Antinuclear / Q000276:immunology*; D003240:Connective Tissue Diseases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D012680:Sensitivity and Specificity
chemical_list: D000974:Antibodies, Antinuclear
keywords: ANA; CTD; Indirect immunofluorescence; LIA; dsDNA ELISA