title: Beyond the known functions of the CCR4-NOT complex in gene expression regulatory mechanisms: New structural insights to unravel CCR4-NOT mRNA processing machinery.
abstract: Large protein assemblies are usually the effectors of major cellular processes. The intricate cell homeostasis network is divided into numerous interconnected pathways, each controlled by a set of protein machines. One of these master regulators is the CCR4-NOT complex, which ultimately controls protein expression levels. This multisubunit complex assembles around a scaffold platform, which enables a wide variety of well-studied functions from mRNA synthesis to transcript decay, as well as other tasks still being identified. Solving the structure of the entire CCR4-NOT complex will help to define the distribution of its functions. The recently published three-dimensional reconstruction of the complex, in combination with the known crystal structures of some of the components, has begun to address this. Methodological improvements in structural biology, especially in cryoelectron microscopy, encourage further structural and protein-protein interaction studies, which will advance our comprehension of the gene expression machinery.
authors: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland. m.ukleja@mail.cryst.bbk.ac.uk.|Faculty of Biology, Department of Genetics and Biotechnology, University of Warsaw, Warsaw, Poland. m.ukleja@mail.cryst.bbk.ac.uk.|Department of Macromolecular Structures, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain. m.ukleja@mail.cryst.bbk.ac.uk.|Institute of Structural and Molecular Biology, University College London and Birkbeck, London, UK. m.ukleja@mail.cryst.bbk.ac.uk.
authors: Marta
authors: M
authors: Ukleja
authors: Department of Macromolecular Structures, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain.
authors: José María
authors: JM
authors: Valpuesta
authors: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.|Faculty of Biology, Department of Genetics and Biotechnology, University of Warsaw, Warsaw, Poland.
authors: Andrzej
authors: A
authors: Dziembowski
authors: Department of Macromolecular Structures, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain. jcuellar@cnb.csic.es.
authors: Jorge
authors: J
authors: Cuellar
mesh_terms: D000818:Animals; D056890:Eukaryota / Q000235:genetics / Q000378:metabolism; D006801:Humans; D046912:Multiprotein Complexes / Q000378:metabolism* / Q000502:physiology; D011487:Protein Conformation; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D046912:Multiprotein Complexes; D012333:RNA, Messenger
keywords: CCR4-NOT; protein degradation; protein expression; structural biology