title: Overloaded and stressed: whole-cell considerations for bacterial synthetic biology.
abstract: The predictability and robustness of engineered bacteria depend on the many interactions between synthetic constructs and their host cells. Expression from synthetic constructs is an unnatural load for the host that typically reduces growth, triggers stresses and leads to decrease in performance or failure of engineered cells. Work in systems and synthetic biology has now begun to address this through new tools, methods and strategies that characterise and exploit host-construct interactions in bacteria. Focusing on work in E. coli, we review here a selection of the recent developments in this area, highlighting the emerging issues and describing the new solutions that are now making the synthetic biology community consider the cell just as much as they consider the construct.
authors: Centre for Synthetic Biology and Innovation, Imperial College London, London, UK; Department of Bioengineering, Imperial College London, London, UK.
authors: Olivier
authors: O
authors: Borkowski
authors: Centre for Synthetic Biology and Innovation, Imperial College London, London, UK; Department of Bioengineering, Imperial College London, London, UK.
authors: Francesca
authors: F
authors: Ceroni
authors: Centre for Synthetic Biology and Innovation, Imperial College London, London, UK; Department of Bioengineering, Imperial College London, London, UK. Electronic address: g.stan@imperial.ac.uk.
authors: Guy-Bart
authors: GB
authors: Stan
authors: Centre for Synthetic Biology and Innovation, Imperial College London, London, UK; Department of Bioengineering, Imperial College London, London, UK. Electronic address: t.ellis@imperial.ac.uk.
authors: Tom
authors: T
authors: Ellis
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods*; D008954:Models, Biological; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D004269:DNA, Bacterial