title: Human eccrine sweat gland cells reconstitute polarized spheroids when subcutaneously implanted with Matrigel in nude mice.
abstract: Increasing evidence indicates that maintenance of cell polarity plays a pivotal role in the regulation of glandular homeostasis and function. We examine the markers for polarity at different time points to investigate the formation of cell polarity during 3D reconstitution of eccrine sweat glands. Mixtures of eccrine sweat gland cells and Matrigel were injected subcutaneously into the inguinal regions of nude mice. At 2, 3, 4, 5 and 6 weeks post-implantation, Matrigel plugs were removed and immunostained for basal collagen IV, lateral β-catenin, lateroapical ZO-1 and apical F-actin. The results showed that the cell polarity of the spheroids appeared in sequence. Formation of basal polarity was prior to lateral, apical and lateroapical polarity. Collagen IV was detected basally at 2 weeks, β-catenin laterally and ZO-1 lateroapically at 3 weeks, and F-actin apically at 4 weeks post-implantation. At week 5 and week 6, the localization and the positive percentage of collagen IV, β-catenin, ZO-1 or F-actin in spheroids was similar to that in native eccrine sweat glands. We conclude that the reconstituted 3D eccrine sweat glands are functional or potentially functional.
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China. lihaihong1051@126.com.|Research Center for Translational Medicine, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China. lihaihong1051@126.com.
authors: Haihong
authors: H
authors: Li
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China.
authors: Mingjun
authors: M
authors: Zhang
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China.
authors: Liyun
authors: L
authors: Chen
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China.
authors: Xuexue
authors: X
authors: Li
authors: Research Center for Translational Medicine, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China.
authors: Bingna
authors: B
authors: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D015415:Biomarkers; D017690:Cell Transplantation / Q000379:methods; D003094:Collagen; D004338:Drug Combinations; D004439:Eccrine Glands / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007797:Laminin; D051379:Mice; D008819:Mice, Nude; D023421:Models, Animal; D061251:Primary Cell Culture; D011509:Proteoglycans; D012038:Regeneration; D013997:Time Factors; D055815:Young Adult
chemical_list: D015415:Biomarkers; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen
keywords: Cell polarity; Eccrine sweat gland; Matrigel; Spheroid; Three-dimensional reconstitution