title: Flow Sorting Plant Chromosomes.
abstract: Nuclear genomes of many important plant species are tremendously complicated to map and sequence. The ability to isolate single chromosomes, which represent small units of nuclear genome, is priceless in many areas of plant research including cytogenetics, genomics, and proteomics. Flow cytometry is the only technique which can provide large quantities of pure chromosome fractions suitable for downstream applications including physical mapping, preparation of chromosome-specific BAC libraries, sequencing, and optical mapping. Here, we describe step-by-step procedure of preparation of liquid suspensions of intact mitotic metaphase chromosomes and their flow cytometric analysis and sorting.
authors: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, 783 71, Olomouc, Czech Republic. vrana@ueb.cas.cz.
authors: Jan
authors: J
authors: Vrána
authors: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, 783 71, Olomouc, Czech Republic.
authors: Petr
authors: P
authors: Cápal
authors: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, 783 71, Olomouc, Czech Republic.
authors: Jarmila
authors: J
authors: Číhalíková
authors: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, 783 71, Olomouc, Czech Republic.
authors: Marie
authors: M
authors: Kubaláková
authors: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, 783 71, Olomouc, Czech Republic.
authors: Jaroslav
authors: J
authors: Doležel
mesh_terms: D032461:Chromosomes, Plant; D005434:Flow Cytometry / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D010944:Plants / Q000235:genetics*
keywords: Cell cycle synchronization; Chromosome isolation; Cytogenetic stocks; FISH; FISHIS; Flow cytometry and sorting; Metaphase accumulation; Plants