title: 64CuS-labeled nanoparticles: a new sentinel-lymph-node-mapping agent for PET-CT and photoacoustic tomography.
abstract: Determining sentinel lymph node (SLN) status is critical to cancer staging and treatment decisions. Currently, in clinical practice,99mTc-radiocolloid-mediated planar scintigraphy and single-photon emission computed tomography (SPECT) are used to guide the biopsy and resection of SLNs. Recently, an emerging technique that combines positron emission tomography (PET) and photoacoustic tomography (PAT; PET-PAT) may offer accurate information in detecting SLNs. Herein, we report a kind of64CuS-labeled nanoparticle (64CuS-NP) for the detection of SLNs with PET-PAT. We subcutaneously injected64CuS-NPs into the rats' forepaw pads. After 24 h, the rats' first draining axillary lymph nodes (i.e. the SLNs) could be clearly visualized with micro-PET (μPET)-CT. Rats were sacrificed after μPET-CT imaging, their axillary lymph nodes were surgically identified, and then PAT was employed to discover64CuS-NP-avid SLNs, which were embedded inside tissues. Biodistribution, autoradiography, and copper staining analyses confirmed the SLNs' high uptake of64CuS-NPs. Our study indicates that64CuS-NPs are a promising dual-function agent for both PET-CT and PAT and could be used with multi-modal imaging strategies such as PET-PAT to identify SLNs in a clinical setting. Copyright © 2016 John Wiley & Sons, Ltd.
authors: Department of Nuclear Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.|Institute of Clinical Nuclear Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.|Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200025, China.
authors: Qiufang
authors: Q
authors: Liu
authors: Department of Cancer Systems Imaging, The University of Texas MD Anderson Cancer Center, Houston, TX 77054, TX, USA.
authors: Min
authors: M
authors: Zhou
authors: Department of Nuclear Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.|Institute of Clinical Nuclear Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.|Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200025, China.
authors: Panli
authors: P
authors: Li
authors: Department of Cancer Systems Imaging, The University of Texas MD Anderson Cancer Center, Houston, TX 77054, TX, USA.
authors: Geng
authors: G
authors: Ku
authors: Department of Nuclear Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.|Institute of Clinical Nuclear Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.|Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200025, China.
authors: Gang
authors: G
authors: Huang
authors: Department of Cancer Systems Imaging, The University of Texas MD Anderson Cancer Center, Houston, TX 77054, TX, USA.
authors: Chun
authors: C
authors: Li
authors: Department of Nuclear Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.|Institute of Clinical Nuclear Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.|Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200025, China.
authors: Shaoli
authors: S
authors: Song
mesh_terms: D000818:Animals; D003300:Copper / Q000737:chemistry; D003301:Copper Radioisotopes / Q000008:administration & dosage / Q000032:analysis*; D008207:Lymphatic Metastasis / Q000000981:diagnostic imaging; D053768:Metal Nanoparticles / Q000008:administration & dosage; D064847:Multimodal Imaging / Q000379:methods*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D009367:Neoplasm Staging / Q000379:methods*; D061088:Photoacoustic Techniques / Q000379:methods; D000072078:Positron Emission Tomography Computed Tomography / Q000379:methods; D019275:Radiopharmaceuticals / Q000008:administration & dosage / Q000493:pharmacokinetics; D051381:Rats; D000071036:Sentinel Lymph Node / Q000000981:diagnostic imaging*
chemical_list: D003301:Copper Radioisotopes; D019275:Radiopharmaceuticals; D003300:Copper; C017846:cupric sulfide
keywords: 64CuS-NPs; PET-CT; SLN imaging; photoacoustic imaging