title: Gold nanoparticles-decorated silver-bipyridine nanobelts for the construction of mediatorless hydrogen peroxide biosensor.
abstract: Au nanoparticles modified with 4-mercaptopyridine and 6-mercapto-1-hexanol were used as coordination agents to prepare a novel hybrid nanomaterial with Ag:4,4'-bipyridine nanobelts. This nanohybrid was employed to modify glassy carbon electrodes and to construct a horseradish peroxidase-based mediatorless amperometric biosensor for H2O2. The electrode, poised at -100mV, exhibited a rapid response within 4s and a linear calibration range from 90pM to 6.5nM H2O2. The biosensor showed a high sensitivity of 283A/Mcm(2) and a very low detection limit of 45pM at a signal-to-noise ratio of 3. The enzyme biosensor showed high stability when stored at 4°C under dry conditions, retaining over 96% and 78% of its initial activity after 15 and 30days of storage at 4°C, respectively.
authors: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040 Madrid, Spain.
authors: Abderrahmane
authors: A
authors: Boujakhrout
authors: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040 Madrid, Spain. Electronic address: paula.diez@ucm.es.
authors: Paula
authors: P
authors: Díez
authors: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040 Madrid, Spain.
authors: Alfredo
authors: A
authors: Sánchez
authors: Department of Organic Chemistry I, Faculty of Chemistry, Complutense University of Madrid, 28040 Madrid, Spain.
authors: Paloma
authors: P
authors: Martínez-Ruíz
authors: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040 Madrid, Spain.
authors: José M
authors: JM
authors: Pingarrón
authors: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040 Madrid, Spain. Electronic address: rvillalonga@quim.ucm.es.
authors: Reynaldo
authors: R
authors: Villalonga
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D000441:Hexanols / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011725:Pyridines / Q000737:chemistry*; D015203:Reproducibility of Results; D059629:Signal-To-Noise Ratio; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: C503488:6-mercapto-1-hexanol; D004800:Enzymes, Immobilized; D000441:Hexanols; D011725:Pyridines; D013438:Sulfhydryl Compounds; C042861:4-thiopyridine; D002244:Carbon; D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C034306:4,4'-bipyridyl
keywords: Biosensor; Gold nanoparticles; Hydrogen peroxide; Nanobelts; Peroxidase