title: Bioactive/Natural Polymeric Scaffolds Loaded with Ciprofloxacin for Treatment of Osteomyelitis.
abstract: Local delivery of antibiotic into injured bone is a demand. In this work, different scaffolds of chitosan (C) with or without bioactive glass (G) were prepared using the freeze-drying technique in 2:1, 1:1, and 1:2 weight ratios. Chitosan scaffolds and selected formulas of chitosan to bioglass were loaded with ciprofloxacin in 5%, 10%, and 20% w/w. Scaffold morphology showed an interconnected porous structure, where the glass particles were homogeneously dispersed in the chitosan matrix. The kinetic study confirmed that the scaffold containing 1:2 weight ratio of chitosan to glass (CG12) showed optimal bioactivity with good compromise between Ca and P uptake capacities and Si release rate. Chitosan/bioactive glass scaffolds showed larger t50values indicating less burst drug release followed by a sustained drug release profile compared to that of chitosan scaffolds. The cell growth, migration, adhesion, and invasion were enhanced onto CG12 scaffold surfaces. Samples of CG12 scaffolds with or without 5% drug induced vascular endothelial growth factor (VEGF), while those containing 10% drug diminished VEGF level. Only CG12 induced the cell differentiation (alkaline phosphatase activity). In conclusion, CG12 containing 5% drug can be considered a biocompatible carrier which would help in the localized osteomyelitis treatment.
authors: Ceramics Department, National Research Centre (NRC), El Bohouth St. 33, 12622 Dokki, Cairo, Egypt. aa.monem@nrc.sci.eg.|Nanomedicine and Tissue Engineering Laboratory, Medical Research Centre of Excellence, NRC, El Bohouth St., 12622 Dokki, Cairo, Egypt. aa.monem@nrc.sci.eg.
authors: Amany A
authors: AA
authors: Mostafa
authors: Chemical Engineering Department, National Research Centre (NRC), El Bohouth St., Dokki, 12622, Cairo, Egypt.|Chemistry Department, American University in Cairo, New Cairo, 11835, Egypt.
authors: Mayyada M H
authors: MMH
authors: El-Sayed
authors: Pharmaceutical Technology Department, National Research Centre, Dokki, Cairo, Egypt.|Pharmaceutics and Pharmaceutical Technology Department, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt, Cairo, Egypt.
authors: Azza A
authors: AA
authors: Mahmoud
authors: Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, NRC, El Bohouth St. 33, Dokki, 12622, Cairo, Egypt. aeldeen7@yahoo.com.|Biochemistry Department, NRC, Dokki, Cairo, Egypt. aeldeen7@yahoo.com.
authors: Amira M
authors: AM
authors: Gamal-Eldeen
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001842:Bone and Bones / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D002939:Ciprofloxacin / Q000008:administration & dosage / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D065546:Drug Liberation; D006801:Humans; D010019:Osteomyelitis / Q000628:therapy*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; C022107:Bioglass; D002939:Ciprofloxacin; D048271:Chitosan
keywords: Saos-2 cells; bone tissue engineering; drug delivery; kinetics; scaffolds