title: Discovery and Validation of Hypermethylated Markers for Colorectal Cancer.
abstract: Colorectal carcinoma (CRC) is one of the most prevalent malignant tumors worldwide. Screening and early diagnosis are critical for the clinical management of this disease. DNA methylation changes have been regarded as promising biomarkers for CRC diagnosis. Here, we map DNA methylation profiling on CRC in six CRCs and paired normal samples using a 450 K bead array. Further analysis confirms the methylation status of candidates in two data sets from the Gene Expression Omnibus. Receiver operating characteristic (ROC) curves are calculated to determine the diagnostic performances. We identify 1549 differentially methylated regions (DMRs) showing differences in methylation between CRC and normal tissue. Two genes (ADD2 and AKR1B1), related to the DMRs, are selected for further validation. ROC curves show that the areas under the curves of ADD2 and AKR1B1 are higher than that of SEPT9, which has been clinically used as a screening biomarker of CRC. Our data suggests that aberrant DNA methylation of ADD2 and AKR1B1 could be potential screening markers of CRC.
authors: Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China; Bio-Bank of Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Jiufeng
authors: 0000-0001-8157-0328
authors: J
authors: Wei
authors: Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China; Bio-Bank of Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Guodong
authors: 0000-0003-0526-3427
authors: G
authors: Li
authors: Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China; Bio-Bank of Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Shuwei
authors: 0000-0003-2520-5857
authors: S
authors: Dang
authors: Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China; Bio-Bank of Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Yuhui
authors: 0000-0002-6386-2308
authors: Y
authors: Zhou
authors: Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China; Bio-Bank of Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Kai
authors: 0000-0002-4300-6715
authors: K
authors: Zeng
authors: Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China; Bio-Bank of Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Ming
authors: 0000-0002-1640-5892
authors: M
authors: Liu
mesh_terms: D000368:Aged; D000449:Aldehyde Reductase / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D058112:Septins / Q000235:genetics; D000099291:Adducins
chemical_list: D000449:Aldehyde Reductase; D014408:Biomarkers, Tumor; D003598:Cytoskeletal Proteins; D058112:Septins; C501062:ADD2 protein, human; D000099291:Adducins; C484670:AKR1B1 protein, human; C474315:SEPTIN9 protein, human