title: Glucagon-Like Peptide 2 Stimulates Postresection Intestinal Adaptation in Preterm Pigs by Affecting Proteins Related to Protein, Carbohydrate, and Sulphur Metabolism.
abstract: Exogenous glucagon-like peptide 2 (GLP-2) stimulates intestinal adaptation after resection in animal models of pediatric short bowel syndrome (SBS). It is unknown whether the molecular mechanisms of such GLP-2 effects are similar to those of postresection spontaneous adaptation. Using preterm pigs as a model, we hypothesized that GLP-2 treatment would change the intestinal proteome within the first week after resection, relative to individuals not resected or resected without GLP-2 treatment.
  
  
  
  Two-day-old preterm pigs were subjected to resection of 50% distal small intestine and fed total parenteral nutrition without (SBS) or with GLP-2 infusion (3.5 µg/kg/h, SBS+GLP-2) for 5 days. The proteome of the remnant proximal intestine was compared among the SBS, SBS+GLP-2, and unresected pigs, through gel-based proteomics.
  
  
  
  Thirty-two proteins with differential expression were identified. Ten of these proteins were affected by the resection alone (ie, SBS vs unresected pigs). Five of these resection-responsive proteins and another 22 proteins were affected by GLP-2 infusion (ie, SBS+GLP-2 vs SBS or unresected pigs). Resection alone mainly affected cellular structural proteins, while the added GLP-2 treatment affected proteins involved in protein processing and the metabolism of protein, carbohydrate, and sulphur.
  
  
  
  In the first days following resection, proteins affected by resection plus GLP-2 treatment differed markedly from those affected by the spontaneous intestinal adaptation following resection alone. Whether more long-term GLP-2 treatment may affect the intestinal proteome following intestinal resection remains unknown.
authors: 1 Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.|2 School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
authors: Pingping
authors: P
authors: Jiang
authors: 1 Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.|Global Research, Novo Nordisk, Måløv, Denmark.
authors: Andreas
authors: A
authors: Vegge
authors: 1 Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.
authors: Thomas
authors: T
authors: Thymann
authors: 2 School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
authors: Jennifer Man-Fan
authors: JM
authors: Wan
authors: 1 Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.
authors: Per Torp
authors: PT
authors: Sangild
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D000831:Animals, Newborn; D004040:Dietary Carbohydrates / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D053767:Glucagon-Like Peptide 2 / Q000494:pharmacology*; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism; D010289:Parenteral Nutrition, Total; D012778:Short Bowel Syndrome / Q000601:surgery*; D013455:Sulfur / Q000378:metabolism; D013552:Swine
chemical_list: D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D053767:Glucagon-Like Peptide 2; D013455:Sulfur
keywords: GLP-2; intestinal adaptation; preterm pig; proteomics; research and diseases; short bowel syndrome