title: Licoricidin, an isoflavonoid isolated from Glycyrrhiza uralensis Fisher, prevents UVA-induced photoaging of human dermal fibroblasts.
abstract: OBJECTIVE
  Licoricidin is an isoflavonoid isolated from Glycyrrhiza uralensis Fisher. In this study, we investigated the effects of licoricidin on photoaging of UVA-irradiated human dermal fibroblasts (HDFs).
  
  
  METHODS
  In vitro reactive oxygen species (ROS) scavenging activity, cellular protective effect and inhibition of elastase activity was determined by Fe3+-EDTA/H2O2systems, photohaemolysis and elastase activity assay, respectively. Anti-oxidative capacity of the compound was evaluated by fluorescent ELISA and 2', 7'-dichlorofluorescin-diacetate (DCF-DA) assay. The expression of protein and phosphorylation was examined using Western blot.
  
  
  RESULTS
  The ROS scavenging activity (OSC50) of licoricidin was 2.77 μM. It was 3.1-fold higher than that of L-ascorbic acid. Its protective effects were confirmed in a study of1O2-induced cellular damage to human erythrocytes. The τ50value of 10 μM of licoricidin was 71.0 min; this was markedly higher than that obtained with α-tocopherol (37.0 min). The elastase inhibitory activity of licoricidin (IC50of 61.2 μM) was 2.1-fold more potent than that of oleanolic acid. Licoricidin markedly reduced the UVA-induced intracellular ROS in a concentration-dependent manner. Western blot revealed that licoricidin attenuated the UVA-dependent induction of MMP-1 protein. Mechanistically, this appeared to be due to licoricidin-dependent inhibition of mitogen-activated protein kinases (MAPK) phosphorylation, which resulted in decreased c-Jun activation and reduced c-Jun and c-Fos expression.
  
  
  CONCLUSIONS
  Licoricidin blocks UVA-induced photoaging via ROS scavenging. This activity converges to limit the activity of MMP-1. These data suggest that licoricidin may be considered as an active ingredient in new topically applied anti-ageing formulations.
authors: Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul, 01811, Korea.
authors: K J
authors: KJ
authors: Kim
authors: Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul, 01811, Korea.
authors: S H
authors: SH
authors: Xuan
authors: Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul, 01811, Korea.
authors: S N
authors: SN
authors: Park
mesh_terms: D001578:Benzopyrans / Q000302:isolation & purification / Q000494:pharmacology*; D004789:Enzyme Activation / Q000528:radiation effects; D004790:Enzyme Induction; D005347:Fibroblasts / Q000187:drug effects; D029882:Glycyrrhiza uralensis / Q000737:chemistry*; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000187:drug effects; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D015595:Skin Aging; D014466:Ultraviolet Rays
chemical_list: D001578:Benzopyrans; D017382:Reactive Oxygen Species; C401293:licoricidin; D020928:Mitogen-Activated Protein Kinases; D020781:Matrix Metalloproteinase 1
keywords: activator protein 1; licoricidin; matrix metalloproteinases; mitogen-activated protein kinases; reactive oxygen species; ultraviolet A