title: An avian influenza H5N1 virus vaccine candidate based on the extracellular domain produced in yeast system as subviral particles protects chickens from lethal challenge.
abstract: Highly pathogenic avian influenza is an on-going problem in poultry and a potential human pandemic threat. Pandemics occur suddenly and vaccine production must be fast and effective to be of value in controlling the spread of the virus. In this study we evaluated the potential of a recombinant protein from the extracellular domain of an H5 hemagglutinin protein produced in a yeast expression system to act as an effective vaccine. Protein production was efficient, with up to 200 mg purified from 1 L of culture medium. We showed that the deletion of the multibasic cleavage site from the protein improves oligomerization and, consequentially, its immunogenicity. We also showed that immunization with this deleted protein protected chickens from challenge with a highly pathogenic avian influenza H5N1 virus. Our results suggest that this recombinant protein produced in yeast may be an effective vaccine against H5N1 virus in poultry.
authors: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
authors: Maria
authors: M
authors: Pietrzak
authors: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
authors: Agnieszka
authors: A
authors: Macioła
authors: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland; Institute of Chemistry, University of Natural Sciences and Humanities, 3 Maja 54, 08-110, Siedlce, Poland.
authors: Konrad
authors: K
authors: Zdanowski
authors: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
authors: Anna Maria
authors: AM
authors: Protas-Klukowska
authors: Department of Poultry Diseases, National Veterinary Research Institute, Partyzantów 57 Avenue, 24-100, Puławy, Poland.
authors: Monika
authors: M
authors: Olszewska
authors: Department of Poultry Diseases, National Veterinary Research Institute, Partyzantów 57 Avenue, 24-100, Puławy, Poland.
authors: Krzysztof
authors: K
authors: Śmietanka
authors: Department of Poultry Diseases, National Veterinary Research Institute, Partyzantów 57 Avenue, 24-100, Puławy, Poland.
authors: Zenon
authors: Z
authors: Minta
authors: Department of Recombinant Vaccines, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kładki 24, 80-822, Gdansk, Poland.
authors: Bogusław
authors: B
authors: Szewczyk
authors: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland. Electronic address: ekopera@ibb.waw.pl.
authors: Edyta
authors: E
authors: Kopera
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D002645:Chickens; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000276:immunology; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D005585:Influenza in Birds / Q000401:mortality / Q000517:prevention & control* / Q000821:virology; D008958:Models, Molecular; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs / Q000276:immunology*; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure; D058425:Vaccines, Virus-Like Particle / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D011994:Recombinant Proteins; D058425:Vaccines, Virus-Like Particle; C109963:hemagglutinin, avian influenza A virus
keywords: Avian influenza; H5N1; Pichia pastoris; Recombinant hemagglutinin