title: A regulatory subunit of protein phosphatase 2A, PPP2R5E, regulates the abundance of microtubule crosslinking factor 1.
abstract: Dynamic changes in microtubule organization are regulated by numerous microtubule-associating proteins and their post-translational modification. Microtubule crosslinking factor 1 (MTCL1) is a recently identified protein that regulates microtubule organization. To obtain further insight into its functions, we searched for proteins that associate with it using mass spectrometry analysis. We found that PPP2R5E, a regulatory subunit of protein phosphatase 2A, interacted with MTCL1. Depletion of PPP2R5E reduced the abundance of MTCL1 abundance, whereas exogenous expression of PPP2R5E increased endogenous MTCL1. Furthermore, inhibition of phosphatase activity by okadaic acid reduced MTCL1, which was restored by the addition of the protease inhibitor MG132. Finally, we show that cells depleted of PPP2R5E and MTCL1 exhibited defects in microtubule organization. Our results suggest that the PPP2R5E phosphatase may contribute to microtubule organization by stabilizing MTCL1.
authors: Division of Cancer Biology, Nagoya University Graduate School of Medicine, Japan.
authors: Toshinori
authors: T
authors: Hyodo
authors: Division of Cancer Biology, Nagoya University Graduate School of Medicine, Japan.
authors: Satoko
authors: S
authors: Ito
authors: Division of Cancer Biology, Nagoya University Graduate School of Medicine, Japan.|Department of Obstetrics and Gynecology Collaborative Research, Bell Research Center, Nagoya University Graduate School of Medicine, Japan.
authors: Eri
authors: E
authors: Asano-Inami
authors: Division of Cancer Biology, Nagoya University Graduate School of Medicine, Japan.
authors: Dan
authors: D
authors: Chen
authors: Division of Cancer Biology, Nagoya University Graduate School of Medicine, Japan. tsenga@med.nagoya-u.ac.jp.
authors: Takeshi
authors: T
authors: Senga
mesh_terms: D006056:Golgi Apparatus / Q000648:ultrastructure; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000648:ultrastructure; D054648:Protein Phosphatase 2 / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: C000604105:MTCL1 protein, human; D008869:Microtubule-Associated Proteins; C525692:PPP2R5E protein, human; D054648:Protein Phosphatase 2
keywords: PPP2R5E; microtubule crosslinking factor 1; microtubules; phosphatase