title: Genome Sequences of Three Apple chlorotic leaf spot virus Isolates from Hawthorns in China.
abstract: The genome sequences of Apple chlorotic leaf spot virus (ACLSV) isolates from three accessions of hawthorns (Crataegus pinnatifida) grown at Shenyang Agricultural University were determined using Illumina RNA-seq. To confirm the assembly data from the de novo sequencing, two ACLSV genomic sequences (SY01 and SY02) were sequenced using the Sanger method. The SY01 and SY02 sequences obtained with the Sanger method showed 99.5% and 99.7% nucleotide identity with the transcriptome data, respectively. The genome sequences of the hawthorn isolates SY01, SY02 and SY03 (GenBank accession nos. KM207212, KU870524 and KU870525, respectively) consisted of 7,543, 7,561 and 7,545 nucleotides, respectively, excluding poly-adenylated tails. Sequence analysis revealed that these hawthorn isolates shared an overall nucleotide identity of 82.8-92.1% and showed the highest identity of 90.3% for isolate YH (GenBank accession no. KC935955) from pear and the lowest identity of 67.7% for isolate TaTao5 (GenBank accession no. EU223295) from peach. Hawthorn isolate sequences were similar to those of 'B6 type' ACLSV. The relationship between ACLSV isolates largely depends upon the host species. This represents the first comparative study of the genome sequences of ACLSV isolates from hawthorns.
authors: College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning, 110866, China.
authors: Wei
authors: http://orcid.org/0000-0003-0266-9552
authors: W
authors: Guo
authors: College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning, 110866, China.
authors: Wenyan
authors: W
authors: Zheng
authors: College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning, 110866, China.
authors: Mei
authors: M
authors: Wang
authors: College of Agronomy, Eastern Liaoning University, 325 Wenhua Road, Dandong, Liaoning, 118003, China.
authors: Xiaohong
authors: X
authors: Li
authors: College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning, 110866, China.
authors: Yue
authors: Y
authors: Ma
authors: College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning, 110866, China.
authors: Hongyan
authors: http://orcid.org/0000-0003-2025-0026
authors: H
authors: Dai
mesh_terms: D000595:Amino Acid Sequence; D027825:Crataegus / Q000235:genetics / Q000821:virology*; D052677:Flexiviridae / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins