title: Effects of waterborne Cu exposure on intestinal copper transport and lipid metabolism of Synechogobius hasta.
abstract: The present study was conducted to explore the effects of waterborne Cu exposure on intestinal Cu transport and lipid metabolism of Synechogobius hasta. S. hasta were exposed to 0, 0.4721 and 0.9442μM Cu, respectively. Sampling occurred on days 0, 21 and 42, respectively. Growth performance, intestinal lipid deposition, Cu content, and activities and mRNA expression of enzymes and genes involved in Cu transport and lipid metabolism were analyzed. Cu exposure decreased WG and SGR on days 21 and 42. Cu exposure increased intestinal Cu and lipid contents. Increased Cu accumulation was attributable to increased enzymatic activities (Cu-ATPase and Cu, Zn-SOD) and genes' (CTR1, CTR2, DMT1, ATP7a, ATP7b, MT1 and MT2) expression involved in Cu transport. Waterborne Cu exposure also increased activities of lipogenic enzymes (6PGD and ICDH on both days 21 and 42, ME on day 42), up-regulated mRNA levels of lipogenic genes (G6PD, 6PGD, ME, ICDH, FAS and ACCa), lipolytic genes (ACCb, CPT I and HSLa) and genes involved in intestinal fatty acid uptake (IFABP and FATP4) on both days 21 and 42. The up-regulation of lipolysis may result from the increased metabolic expenditure for detoxification and maintenance of the normal body functions in a response to Cu exposure. Meantime, Cu exposure increased lipogenesis and fatty acid uptake, leading to net lipid accumulation in the intestine despite increased lipolysis. To our knowledge, this is the first report involved in intestinal lipid metabolism in combination with intestinal Cu absorption following waterborne Cu exposure, which provides new insights and evidence into Cu toxicity in fish.
authors: Laboratory of Nutrition and Feed Formulation for Aquatic Economic Animals, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
authors: Feng
authors: F
authors: Chen
authors: Laboratory of Nutrition and Feed Formulation for Aquatic Economic Animals, Fishery College, Huazhong Agricultural University, Wuhan 430070, China; Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China; Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan 430070, China. Electronic address: luozhi99@mail.hzau.edu.cn.
authors: Zhi
authors: Z
authors: Luo
authors: Laboratory of Nutrition and Feed Formulation for Aquatic Economic Animals, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
authors: Guang-Hui
authors: GH
authors: Chen
authors: Laboratory of Nutrition and Feed Formulation for Aquatic Economic Animals, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
authors: Xi
authors: X
authors: Shi
authors: Postgraduate Research Base, Panjin Guanghe Fishery Co. Ltd., Panjin 124200, China.
authors: Xu
authors: X
authors: Liu
authors: Laboratory of Nutrition and Feed Formulation for Aquatic Economic Animals, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
authors: Yu-Feng
authors: YF
authors: Song
authors: Laboratory of Nutrition and Feed Formulation for Aquatic Economic Animals, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
authors: Ya-Xiong
authors: YX
authors: Pan
mesh_terms: D000818:Animals; D003300:Copper / Q000633:toxicity*; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects*; D010473:Perciformes / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D004798:Enzymes; D029941:Fish Proteins; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D003300:Copper
keywords: Copper; Copper transport; Fatty acid uptake; Lipid metabolism; Synechogobius hasta