title: Entrapping quercetin in silica/polyethylene glycol hybrid materials: Chemical characterization and biocompatibility.
abstract: Sol-gel synthesis was exploited to entrap quercetin, a natural occurring antioxidant polyphenol, in silica-based hybrid materials, which differed in their polyethylene glycol (PEG) content (6, 12, 24 and 50wt%). The materials obtained, whose nano-composite nature was ascertained by Scanning Electron Microscopy (SEM), were chemically characterized by Fourier Transform InfraRed (FT-IR) and UV-Vis spectroscopies. The results prove that a reaction between the polymer and the drug occurred. Bioactivity tests showed their ability to induce hydroxyapatite nucleation on the sample surfaces. The direct contact method was applied to screen the cytotoxicity of the synthetized materials towards fibroblast NIH 3T3 cells, commonly used for in vitro biocompatibility studies, and three nervous system cell lines (neuroblastoma SH-SY5Y, glioma U251, and pheochromocytoma PC12 cell lines), adopted as models in oxidative stress related studies. Using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay NIH 3T3 proliferation was assessed and the morphology was not compromised by direct exposure to the materials. Analogously, PC-12, and U-251 cell lines were not affected by new materials. SH-SY5Y appeared to be the most sensitive cell line with cytotoxic effects of 20-35%.
authors: Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031 Aversa, Italy. Electronic address: michelina.catauro@unina2.it.
authors: Michelina
authors: M
authors: Catauro
authors: Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031 Aversa, Italy.
authors: Flavia
authors: F
authors: Bollino
authors: Department Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, 81100 Caserta, Italy.
authors: Paola
authors: P
authors: Nocera
authors: Department Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, 81100 Caserta, Italy.
authors: Simona
authors: S
authors: Piccolella
authors: Department Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, 81100 Caserta, Italy.
authors: Severina
authors: S
authors: Pacifico
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D016716:PC12 Cells; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology
chemical_list: D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D011794:Quercetin; C000595209:polyethylene glycol 1000
keywords: Cytotoxicity; FT-IR spectroscopy; Polyethylene glycol 400; Quercetin; Sol-gel hybrid materials