title: Agrobacterium rhizogenes rolB gene affects photosynthesis and chlorophyll content in transgenic tomato (Solanum lycopersicum L.) plants.
abstract: Insertion of Agrobacterium rhizogenes rolB gene into plant genome affects plant development, hormone balance and defence. However, beside the current research, the overall transcriptional response and gene expression of rolB as a modulator in plant is unknown. Transformed rolB tomato plant (Solanum lycopersicum L.) cultivar Tondino has been used to investigate the differential expression profile. Tomato is a well-known model organism both at the genetic and molecular level, and one of the most important commercial food crops in the world. Through the construction and characterization of a cDNA subtracted library, we have investigated the differential gene expression between transgenic clones of rolB and control tomato and have evaluated genes specifically transcribed in transgenic rolB plants. Among the selected genes, five genes encoding for chlorophyll a/b binding protein, carbonic anhydrase, cytochrome b6/f complex Fe-S subunit, potassium efflux antiporter 3, and chloroplast small heat-shock protein, all involved in chloroplast function, were identified. Measurement of photosynthesis efficiency by the level of three different photosynthetic parameters (Fv/Fm, rETR, NPQ) showed rolB significant increase in non-photochemical quenching and a, b chlorophyll content. Our results point to highlight the role of rolB on plant fitness by improving photosynthesis.
authors: Dipartimento di Biologia, Università degli Studi di Firenze, via Madonna del Piano 6, 50019, Sesto Fiorentino (FI), Italy. Electronic address: p.bettini@unifi.it.
authors: Priscilla P
authors: PP
authors: Bettini
authors: Dipartimento di Biologia, Università degli Studi di Firenze, via Madonna del Piano 6, 50019, Sesto Fiorentino (FI), Italy. Electronic address: m.marvasi@mdx.ac.uk.
authors: Massimiliano
authors: M
authors: Marvasi
authors: Dipartimento di Biologia, Università degli Studi di Firenze, via Madonna del Piano 6, 50019, Sesto Fiorentino (FI), Italy. Electronic address: fabiola.fani@unifi.it.
authors: Fabiola
authors: F
authors: Fani
authors: Dipartimento di Biologia, Università degli Studi di Firenze, via Madonna del Piano 6, 50019, Sesto Fiorentino (FI), Italy. Electronic address: luigi.lazzara@unifi.it.
authors: Luigi
authors: L
authors: Lazzara
authors: Dipartimento di Biologia, Università degli Studi di Firenze, via Madonna del Piano 6, 50019, Sesto Fiorentino (FI), Italy. Electronic address: cosieli@hotmail.com.
authors: Elena
authors: E
authors: Cosi
authors: Dipartimento di Biologia, Università degli Studi di Firenze, via Madonna del Piano 6, 50019, Sesto Fiorentino (FI), Italy. Electronic address: Lorenzo.Melani@solvay.com.
authors: Lorenzo
authors: L
authors: Melani
authors: Dipartimento di Biologia e Biotecnologie, Sapienza Università di Roma, P.le A. Moro 5, 00185 Roma, Italy. Electronic address: marialuisa.mauro@uniroma1.it.
authors: Maria Luisa
authors: ML
authors: Mauro
mesh_terms: D060054:Agrobacterium / Q000235:genetics*; D002734:Chlorophyll / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D005798:Genes, Bacterial; D018551:Solanum lycopersicum / Q000235:genetics*; D010641:Phenotype; D010788:Photosynthesis; D030821:Plants, Genetically Modified; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D002734:Chlorophyll
keywords: Agrobacterium rhizogenes rolB gene; Photosynthesis; Suppression subtractive hybridization; Tomato