title: Reprogramming A375 cells to induced‑resembled neuronal cells by structured overexpression of specific transcription genes.
abstract: Induced-resembled neuronal cells (irNCs) are generated by reprogramming human melanoma cells through the introduction of key transcription factors, providing novel concepts in the treatment of malignant tumor cells and making it possible to supply neural cells for laboratory use. In the present study, irNCs were derived from A375 cells by inducing the 'forced' overexpression of specific genes, including achaete‑scute homolog 1 (Ascl1), neuronal differentiation factor 1 (Neurod1), myelin transcription factor 1 (Myt1), brain protein 2 (Brn2, also termed POU3F2) and human brain‑derived neurotrophic factor (h‑BDNF). irNCs induced from A375 cells express multiple neuronal markers and fire action potentials, exhibiting properties similar to those of motor neurons. The reprogramming procedure comprised reverse transcription‑polymerase chain reaction and immunofluorescence staining; furthermore, electrophysiological profiling demonstrated the characteristics of the induced‑resembled neurons. The present study obtained a novel type of human irNC from human melanoma, which secreted BDNF continuously, providing a model for neuron‑like cells. Thus, irNCs offer promise in investigating various neural diseases by using neural‑like cells derived directly from the patient of interest.
authors: Department of Neurosurgery, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China.
authors: Hengzhu
authors: H
authors: Zhang
authors: Department of Neurosurgery, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China.
authors: Min
authors: M
authors: Wei
authors: Department of Neurosurgery, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China.
authors: Yangyang
authors: Y
authors: Jiang
authors: Department of Neurosurgery, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China.
authors: Xiaodong
authors: X
authors: Wang
authors: Department of Neurosurgery, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China.
authors: Lei
authors: L
authors: She
authors: Department of Neurosurgery, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China.
authors: Zhengcun
authors: Z
authors: Yan
authors: Department of Neurosurgery, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China.
authors: Lun
authors: L
authors: Dong
authors: Department of Neurosurgery, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China.
authors: Lujun
authors: L
authors: Pang
authors: Department of Neurosurgery, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China.
authors: Xingdong
authors: X
authors: Wang
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics; D002460:Cell Line; D045744:Cell Line, Tumor; D065150:Cellular Reprogramming; D004268:DNA-Binding Proteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D008545:Melanoma / Q000235:genetics; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D050809:POU Domain Factors / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D019208:Brain-Derived Neurotrophic Factor; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D050809:POU Domain Factors; D014157:Transcription Factors; C065500:ASCL1 protein, human; C078341:MYT1 protein, human; C093970:NEUROD1 protein, human; C085076:transcription factor Brn-2