title: Expanding specificity of class I restricted CD8+T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine.
abstract: The immune response to the highly acute foot-and-mouth disease virus (FMDV) is routinely reported as a measure of serum antibody. However, a critical effector function of immune responses combating viral infection of mammals is the cytotoxic T lymphocyte (CTL) response mediated by virus specific CD8 expressing T cells. This immune mechanism arrests viral spread by killing virus infected cells before new, mature virus can develop. We have previously shown that infection of swine by FMDV results in a measurable CTL response and have correlated CTL killing of virus-infected cells with specific class I major histocompatibility complex (MHC) tetramer staining. We also showed that a modified replication defective human adenovirus 5 vector expressing the FMDV structural proteins (Ad5-FMDV-T vaccine) targets the induction of a CD8+CTL response with a minimal humoral response. In this report, we show that the specificity of the CD8+T cell response to Ad5-FMDV-T varies between cohorts of genetically identical animals. Further, we demonstrate epitope specificity of CD8+T cells expands following multiple immunizations with this vaccine.
authors: Plum Island Animal Disease Center, Agricultural Research Service, USDA, Greenport, NY, USA; National Veterinary Institute and Centre for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark.
authors: Lasse E
authors: LE
authors: Pedersen
authors: Plum Island Animal Disease Center, Agricultural Research Service, USDA, Greenport, NY, USA; Department of Animal and Veterinary Sciences, University of Vermont, Burlington, VT, USA.
authors: Jared R
authors: JR
authors: Patch
authors: Plum Island Animal Disease Center, Agricultural Research Service, USDA, Greenport, NY, USA.
authors: Mary
authors: M
authors: Kenney
authors: Plum Island Animal Disease Center, Agricultural Research Service, USDA, Greenport, NY, USA; Department of Animal and Veterinary Sciences, University of Vermont, Burlington, VT, USA.
authors: Raisa A
authors: RA
authors: Glabman
authors: National Veterinary Institute and Centre for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark; Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, San Martín, Buenos Aires, Argentina.
authors: Morten
authors: M
authors: Nielsen
authors: National Veterinary Institute and Centre for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark.
authors: Gregers
authors: G
authors: Jungersen
authors: University of Copenhagen, Copenhagen, Denmark.
authors: Soren
authors: S
authors: Buus
authors: Plum Island Animal Disease Center, Agricultural Research Service, USDA, Greenport, NY, USA. Electronic address: wtgolde@aol.com.
authors: William T
authors: WT
authors: Golde
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D000939:Epitopes; D018984:Epitopes, T-Lymphocyte; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D005822:Genetic Vectors; D006801:Humans; D013552:Swine; D014611:Vaccination / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000939:Epitopes; D018984:Epitopes, T-Lymphocyte; D014614:Vaccines, Synthetic; D014765:Viral Vaccines
keywords: Class I; Cytotoxic T lymphocytes; MHC; MHC tetramers; SLA; T cell epitopes