title: Novel interactions between erythroblast macrophage protein and cell migration.
abstract: Erythroblast macrophage protein is a novel protein known to mediate attachment of erythroid cells to macrophages to form erythroblastic islands in bone marrow during erythropoiesis. Emp-null macrophages are small with round morphologies, and lack cytoplasmic projections which imply immature structure. The role of Emp in macrophage development and function is not fully elucidated. Macrophages perform varied functions (e.g. homeostasis, erythropoiesis), and are implicated in numerous pathophysiological conditions such as cellular malignancy. The objective of the current study is to investigate the interaction of Emp with cytoskeletal- and cell migration-associated proteins involved in macrophage functions. A short hairpin RNA lentiviral system was use to down-regulate the expression of Emp in macrophage cells. A cell migration assay revealed that the relocation of macrophages was significantly inhibited when Emp expression was decreased. To further analyze changes in gene expression related to cell motility, PCR array was performed by down-regulating Emp expression. The results indicated that expression of mitogen-activated protein kinase 1 and thymoma viral proto-oncogene 1 were significantly higher when Emp was down-regulated. The results implicate Emp in abnormal cell motility, thus, warrants to assess its role in cancer where tumor cell motility is required for invasion and metastasis.
authors: Department of Physical Sciences, Forensic Science Program, Alabama State University, Montgomery, AL 36104, United States. Electronic address: gjavan@alasu.edu.
authors: Gulnaz T
authors: GT
authors: Javan
authors: Department of Physical Sciences, Forensic Science Program, Alabama State University, Montgomery, AL 36104, United States.
authors: Ismail
authors: I
authors: Can
authors: Department of Biological Sciences, Alabama State University, Montgomery, AL 36104, United States.
authors: Fred
authors: F
authors: Yeboah
authors: Department of Exercise Science and Community Health, University of West Florida, Pensacola, FL 32514, United States.
authors: Youngil
authors: Y
authors: Lee
authors: Department of Biological Sciences, Alabama State University, Montgomery, AL 36104, United States. Electronic address: ssoni@alasu.edu.
authors: Shivani
authors: S
authors: Soni
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D002465:Cell Movement / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D004900:Erythroblasts / Q000378:metabolism / Q000473:pathology; D041905:Erythroid Cells / Q000378:metabolism; D004920:Erythropoiesis; D005786:Gene Expression Regulation; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D000067996:RAW 264.7 Cells; D034741:RNA, Small Interfering / Q000494:pharmacology
chemical_list: D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D034741:RNA, Small Interfering; C513480:erythroblast macrophage protein, mouse; C514862:Akt1 protein, mouse; D051057:Proto-Oncogene Proteins c-akt; C535148:Mapk1 protein, mouse; D019950:Mitogen-Activated Protein Kinase 1
keywords: Erythroblast macrophage protein (Emp); Macrophage; Migration