title: Effects and mechanisms of curcumin on the hemodynamic variablesof isolated perfused rat hearts.
abstract: OBJECTIVE
  There is no information on the dose-response relationship of curcumin on the hemodynamic variables of the heart at the organ level in isolated perfused rat hearts. We aimed to investigate the effects and mechanisms of curcumin on the hemodynamic variables of isolated perfused rat hearts.
  
  
  METHODS
  Rats were randomly divided into 9 groups. The isolated rat heart was retrogradely perfused with modified Krebs-Henseleit solution. After the stabilization period, each group was administered one of the following treatments for 25 min: saline, dimethyl sulfoxide, and curcumin (0.2 µM, 1 µM, and 5 µM); atropine (1 µM); atropine (1 µM) + curcumin (1 µM); L-NAME (100 µM); or L-NAME (100 µM) + curcumin (1 µM). Hemodynamic variables of the heart were measured.
  
  
  RESULTS
  Curcumin at dose of 1 µM decreased the heart rate (from 271 ± 11.1 to 200.4 ± 14.3 beats/min, P = 0.011) but increased end-diastolic pressure (from 7.0 ± 0.4 to 54.6 ± 7.9 mmHg, P = 0.0008). A dose of 5 µM curcumin caused a decrease in the developed pressure (from 87.58 ± 9.0 to 65.40 ± 7.0 mmHg, P = 0.047) but an increase in the end-diastolic pressure (from 6.8 ± 0.6 to 48.9 ± 7.7 mmHg, P = 0.005). Atropine (1 µM) reversed the effects of curcumin on the heart.
  
  
  CONCLUSIONS
  Our results suggest that curcumin produces dose-dependent negative chronotropic and inotropic effects in isolated perfused rat hearts.
authors: Department of Physiology, Faculty of Medicine, Abant İzzet Baysal University, Bolu, Turkey.
authors: Erkan
authors: E
authors: Kilinç
authors: Department of Physiology, Faculty of Medicine, Eskişehir Osmangazi University, Eskişehir, Turkey.
authors: Ziya
authors: Z
authors: Kaygisiz
authors: Department of Physiology, Faculty of Medicine, Abant İzzet Baysal University, Bolu, Turkey.
authors: Bedri Selim
authors: BS
authors: Benek
authors: Department of Physiology, Faculty of Medicine, Abant İzzet Baysal University, Bolu, Turkey.
authors: Kenan
authors: K
authors: Gümüştekin
mesh_terms: D000818:Animals; D001794:Blood Pressure; D003474:Curcumin / Q000494:pharmacology*; D006321:Heart; D006339:Heart Rate; D006439:Hemodynamics; D051381:Rats
chemical_list: D003474:Curcumin
keywords: +dP/dtmax; cardiac contractility; curcumin; heart rate; isolated perfused heart