title: Competitive colonization of prosthetic surfaces by staphylococcus aureus and human cells.
abstract: Implantation of a biomaterial provides an adhesion substratum both to host cell integration and to contaminating bacteria. We studied simultaneous competitive adhesion of Staphylococcus aureus in serial 1:10 dilutions of 108colony forming units (CFU)/mL and human osteogenic sarcoma (SaOS-2) or primary osteoblast (hOB) cells, both 1x105cells/mL, to the surfaces of titanium, polydimethylsiloxane and polystyrene. The bacterial adherence and human cell proliferation, cytotoxicity and production of reactive oxygen species (ROS) were studied using fluorometric (fluorescent microscopy and flow cytometry) and colorimetric methods (MTT, LDH and crystal violet). The bacterial cell viability was also evaluated using the drop plate method. The presence of bacteria resulted in reduced adherence of human cells to the surface of the biomaterials, increased production of ROS, and into increased apoptosis. On the other hand, the presence of either type of human cells was associated with a reduction of bacterial colonization of the biomaterial with Staphylococcus aureus. These results suggest that increasing colonization of the biomaterial surface in vitro by one negatively affects colonization by the other. Host cell integration to an implant surface reduces bacterial contamination, which opens novel opportunities for the design of infection-resistant biomaterials in current implantology and future regenerative medicine. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 62-72, 2017.
authors: Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: Ramón
authors: 0000-0002-9816-3208
authors: R
authors: Pérez-Tanoira
authors: Medicine, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: Xia
authors: X
authors: Han
authors: ORTON Research Institute, Helsinki, Finland.|ORTON Orthopedic Hospital, Helsinki, Finland.
authors: Antti
authors: A
authors: Soininen
authors: Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: Antti A
authors: AA
authors: Aarnisalo
authors: ORTON Research Institute, Helsinki, Finland.|ORTON Orthopedic Hospital, Helsinki, Finland.
authors: Veli-Matti
authors: VM
authors: Tiainen
authors: Medicine, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.|Rheumatology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: Kari K
authors: KK
authors: Eklund
authors: Clinical Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain.
authors: Jaime
authors: J
authors: Esteban
authors: Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: Teemu J
authors: TJ
authors: Kinnari
mesh_terms: D045744:Cell Line, Tumor; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006801:Humans; D020007:Implants, Experimental / Q000382:microbiology*; D011137:Polystyrenes / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000254:growth & development*
chemical_list: D004129:Dimethylpolysiloxanes; D011137:Polystyrenes; C013830:baysilon
keywords: bacterial adherence; biofilm; biomaterial; cellular oxidative stress; race for the surface