title: Biomimetic mineralization of nano-sized, needle-like hydroxyapatite with ultrahigh capacity for lysozyme adsorption.
abstract: Because of its superior biocompatibility, hydroxyapatite (HA) has been widely exploited as a promising vehicle to deliver a broad range of therapeutics in a variety of biological systems. Herein, we report a biomimetic process to prepare nano-sized, colloidal stable HA with needle-like morphology by using carboxymethyl cellulose (CMC) as the template. It was revealed that the needle-like HA was transformed from the spherical amorphous calcium phosphate (ACP) nanoparticles after a 14-day period of aging under ambient conditions. The needle-like HA/CMC exhibited an ultra-high lysozyme adsorption capacity up to 930-940mg/g. Moreover, a sustained and pH-sensitive release of adsorbed lysozyme from HA/CMC was evidenced. Therefore, our biomimetic needle-like HA/CMC nanoparticles hold great potential in serving as an efficient carrier for the delivery and controlled release of lysozyme.
authors: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China; School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China.
authors: Yi
authors: Y
authors: Ma
authors: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China.
authors: Juan
authors: J
authors: Zhang
authors: School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China.
authors: Shanshan
authors: S
authors: Guo
authors: School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China.
authors: Jie
authors: J
authors: Shi
authors: School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China.
authors: Wenying
authors: W
authors: Du
authors: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China.
authors: Zheng
authors: Z
authors: Wang
authors: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China.
authors: Ling
authors: L
authors: Ye
authors: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China. Electronic address: weigu@ccmu.edu.cn.
authors: Wei
authors: W
authors: Gu
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D017886:Durapatite; D009113:Muramidase; D002266:Carboxymethylcellulose Sodium
keywords: Biomimetic; Carboxymethyl cellulose (CMC); Hydroxyapatite (HA); Lysozyme adsorption; Phase transformation