title: The Heidelberg Screen for Pattern Mutants of Drosophila: A Personal Account.
abstract: In large-scale mutagenesis screens performed in 1979-1980 at the EMBL in Heidelberg, we isolated mutations affecting the pattern or structure of the larval cuticle in Drosophila. The 600 mutants we characterized could be assigned to 120 genes and represent the majority of such genes in the genome. These mutants subsequently provided a rich resource for understanding many fundamental developmental processes, such as the transcriptional hierarchies controlling segmentation, the establishment of cell states by signaling pathways, and the differentiation of epithelial cells. Most of the Heidelberg genes are now molecularly known, and many of them are conserved in other animals, including humans. Although the screens were initially driven entirely by curiosity, the mutants now serve as models for many human diseases. In this review, we describe the rationale of the screening procedures and provide a classification of the genes on the basis of their initial phenotypes and the subsequent molecular analyses.
authors: Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544; email: efw@princeton.edu.
authors: Eric
authors: E
authors: Wieschaus
authors: Max-Planck-Institut für Entwicklungsbiologie, 72076 Tübingen, Germany; email: cnv@tuebingen.mpg.de.
authors: Christiane
authors: C
authors: Nüsslein-Volhard
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics*; D017344:Genes, Insect; D005820:Genetic Testing; D016296:Mutagenesis / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
keywords: Drosophila; autobiography; embryonic lethal mutations; larval cuticle; pattern formation; saturation screens