title: The O-antigen of Plesiomonas shigelloides serotype O36 containing pseudaminic acid.
abstract: The structure of the repeating unit of O-antigen of Plesiomonas shigelloides serotype O36 has been investigated by1H and13C NMR spectroscopy, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and chemical methods. The new structure of trisaccharide has been established: [Formula: see text] These trisaccharide O-antigen units substitute the core undecasaccharide at C-4 of the β-D-GlcpNAc residue. The core oligosaccharide and lipid A are identical with these of the serotype O17 (PCM 2231) (Maciejewska, A., Lukasiewicz, J., Kaszowska, M., Jachymek, W., Man-Kupisinska, A.; Lugowski, C. Mar. Drugs.2013, 11 (2), 440-454; Lukasiewicz, J., Dzieciatkowska, M., Niedziela, T., Jachymek, W., Augustyniuk, A., Kenne, L., Lugowski, C. Biochemistry, 2006, 45, 10434-10447).
authors: Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114, Wroclaw, Poland. Electronic address: marta.kaszowska@iitd.pan.wroc.pl.
authors: Marta
authors: M
authors: Kaszowska
authors: Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114, Wroclaw, Poland.
authors: Katarina
authors: K
authors: Stojkovic
authors: Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114, Wroclaw, Poland.
authors: Tomasz
authors: T
authors: Niedziela
authors: Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114, Wroclaw, Poland; Department of Biotechnology and Molecular Biology, University of Opole, PL-45-035, Opole, Poland.
authors: Czeslaw
authors: C
authors: Lugowski
mesh_terms: D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics; D016981:Plesiomonas / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D065288:Serogroup
chemical_list: D019081:O Antigens
keywords: MALDI-TOF MS; NMR; O-antigen; Plesiomonas shigelloides