title: Patterns of genetic structure and evidence of gene flow among Tunisian Citrus species based on informative nSSR markers.
abstract: This study investigates the extent of genetic diversity, phylogenetic relationships and the amount of gene flow among Tunisian Citrus species based on a set of 15 informative nuclear SSR molecular markers. Genotyping data highlighted an allelic richness among Tunisian Citrus species and has allowed the detection of 168 alleles among them 104.19 were effective. The partition of the total genetic diversity (HT=0.832) showed that the highest amount of variation within the Citrus species is HS=0.550, while the relative amount of the between-species genetic diversity GST does not exceed 0.338. This pattern of genetic structure was supported by low-to-moderate FST pairwise values and the presence of a gene flow (Nm) among the eight Citrus species. The lowest genetic differentiation was revealed between the species C. sinensis and C. insitorum (FST=0.111, Nm=1.99), while the highest genetic differentiation was recorded between the species C. aurantifolia and C. paradisi (FST=0.367, Nm=0.43). The established Neighbor Joining analysis showed that all genotypes were widely discriminated and clearly pooled according to their species of origin, with minor exceptions.
authors: Laboratoire de physiologie moléculaire des plantes, centre de biotechnologie Borj Cedria, BP 901, 2050 Hammam-Lif, Tunisia.
authors: Meriam
authors: M
authors: Ben Romdhane
authors: Laboratoire de biotechnologie et valorisation des bio-géo ressources (LR11ES31), Institut supérieur de biotechnologie, université de La Manouba, BiotechPole Sidi Thabet, 2020 Sidi Thabet, Ariana, Tunisia.
authors: Leila
authors: L
authors: Riahi
authors: Laboratoire de physiologie moléculaire des plantes, centre de biotechnologie Borj Cedria, BP 901, 2050 Hammam-Lif, Tunisia.
authors: Ayet
authors: A
authors: Selmi
authors: Laboratoire de physiologie moléculaire des plantes, centre de biotechnologie Borj Cedria, BP 901, 2050 Hammam-Lif, Tunisia. Electronic address: zoghlami_n@yahoo.fr.
authors: Nejia
authors: N
authors: Zoghlami
mesh_terms: D002957:Citrus / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D051456:Gene Flow / Q000235:genetics*; D005788:Gene Pool; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D018895:Microsatellite Repeats; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity; D014416:Tunisia
chemical_list: D018744:DNA, Plant; D004276:DNA, Satellite; D005819:Genetic Markers
keywords: Citrus species; Gene flow; Genetic structure; Phylogeny; nSSR