title: Gene expression in Catla catla (Hamilton) subjected to acute and protracted doses of gamma radiation.
abstract: Studies on transcriptional modulation after gamma radiation exposure in fish are limited. Cell cycle perturbations and expression of apoptotic genes were investigated in the fish, Catla catla after acute and protracted exposures to gamma radiation over a 90day period. Significant changes in gene expression were observed between day 1 and 90 post-exposure. Gamma radiation induced a significant down-regulation of target genes gadd45α, cdk1 and bcl-2 from day 1 to day 3 after protracted exposure, whereas it persists till day 6 upon acute exposure. From day 12 onwards, Gadd45α, cdk1 and bcl-2 genes were up-regulated following protracted exposure, indicating DNA repair, cell-cycle arrest and apoptosis. There exists a linear correlation between these genes (gadd45α - r=0.85, p=0.0073; cdk1 - r=0.86, p=0.0053; bcl-2 - r=0.89, p=0.0026) at protracted exposures. This is the first report on the dual role of bcl-2 gene in fish exposed to acute and protracted radiation and correlation among the aforementioned genes that work in concert to promote 'repair' and 'death' circuitries in fish blood cells.
authors: Radiological Safety Division, Indira Gandhi Centre for Atomic Research (IGCAR), Kalpakkam, Tamilnadu 603102 India. Electronic address: aquatox1982@gmail.com.
authors: S
authors: S
authors: Anbumani
authors: Radiological Safety Division, Indira Gandhi Centre for Atomic Research (IGCAR), Kalpakkam, Tamilnadu 603102 India. Electronic address: marynmk@gmail.com.
authors: Mary N
authors: MN
authors: Mohankumar
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D059447:Cell Cycle Checkpoints / Q000528:radiation effects; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D003530:Cyprinidae / Q000235:genetics; D004260:DNA Repair / Q000528:radiation effects; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D018797:Cell Cycle Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger
keywords: Apoptosis; Catla catla; Cell cycle; Gamma radiation; Gene expression; RT-PCR