title: High-Resolution Molecular Typing of Trypanosoma cruzi in 2 Large Outbreaks of Acute Chagas Disease in Colombia.
abstract: Oral transmission of Trypanosoma cruzi has gained relevance because of its association with high morbidity and lethality rates. This transmission route is responsible for maintaining the infection of the parasite in sylvatic cycles, and human cases have been associated mainly with the consumption of food contaminated with triatomine feces or didelphid secretions. Several ecological changes allow the intrusion of sylvatic reservoirs and triatomines to the domestic environments with subsequent food contamination. Here, high-resolution molecular tools were used to detect and genotype T. cruzi across humans, reservoirs, and insect vectors in 2 acute outbreaks of presumptive oral transmission in eastern Colombia.
authors: Grupo de Investigaciones Microbiológicas-UR (GIMUR), Programa de Biología, Facultad de Ciencias Naturales y Matemáticas, Universidad el Rosario Red Chagas Colombia Grupo de Parasitología, Instituto Nacional de Salud.
authors: Carolina
authors: C
authors: Hernández
authors: Ministerio de Salud y protección Social, Bogotá, Colombia.
authors: Mauricio Javier
authors: MJ
authors: Vera
authors: Red Chagas Colombia Grupo de Parasitología, Instituto Nacional de Salud Department of Infectious Disease Epidemiology, Imperial College London, United Kingdom.
authors: Zulma
authors: Z
authors: Cucunubá
authors: Grupo de Parasitología, Instituto Nacional de Salud.
authors: Carolina
authors: C
authors: Flórez
authors: Red Chagas Colombia Laboratorio Biología y Control de Enfermedades Infecciosas (BCEI), Universidad de Antioquia, Medellín.
authors: Omar
authors: O
authors: Cantillo
authors: Laboratorio Departamental de Salud del Meta, Villavicencio.
authors: Luz Stella
authors: LS
authors: Buitrago
authors: Laboratorio Departamental de Salud del Meta, Villavicencio.
authors: Marina Stella
authors: MS
authors: González
authors: Grupo de Entomología, Instituto Nacional de Salud, Bogotá
authors: Susanne
authors: S
authors: Ardila
authors: Laboratorio Departamental de Salud de Casanare, Yopal, Colombia.
authors: Liliana Zuleta
authors: LZ
authors: Dueñas
authors: Ministerio de Salud y protección Social, Bogotá, Colombia.
authors: Rubén
authors: R
authors: Tovar
authors: Ministerio de Salud y protección Social, Bogotá, Colombia.
authors: Luis Fernando
authors: LF
authors: Forero
authors: Grupo de Investigaciones Microbiológicas-UR (GIMUR), Programa de Biología, Facultad de Ciencias Naturales y Matemáticas, Universidad el Rosario.
authors: Juan David
authors: JD
authors: Ramírez
mesh_terms: D000208:Acute Disease; D000328:Adult; D000818:Animals; D014355:Chagas Disease / Q000469:parasitology*; D003105:Colombia / Q000453:epidemiology; D016054:DNA, Protozoan / Q000235:genetics; D004196:Disease Outbreaks; D004197:Disease Reservoirs / Q000469:parasitology; D005260:Female; D005838:Genotype; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D058889:Molecular Typing / Q000379:methods*; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan
keywords: Chagas disease; Trypanosoma cruzi; canines; food; opossums; oral transmission; outbreaks; triatomines