title: Elevated methylation of CMTM3 promoter in the male laryngeal squamous cell carcinoma patients.
abstract: OBJECTIVE
  CKLF-like MARVEL transmembrane domain containing 3 (CMTM3), as a tumor suppressor gene, plays an important role in the suppression of cell growth and apoptosis. The goal of our study is to investigate the association between CMTM3 promoter methylation and laryngeal squamous cell carcinoma (LSCC).
  
  
  METHODS
  Using the bisulfite pyrosequencing technology, DNA methylation levels of seven CpG sites in CMTM3 promoter are measured in tumor tissues and their adjacent tissues of 76 male LSCC patients.
  
  
  RESULTS
  Our results reveal a significantly elevated promoter methylation of CMTM3 in tumor tissues compared with their adjacent tissues (P<0.001). A breakdown analysis by age shows that significant association of CMTM3 promoter methylation with cancer risk is specific to the LSCC patients older than 55years (P<0.001) but not in the younger patients (P=0.305). Moreover, the association is only observed in the LSCC patients with smoking behavior (P=0.001). Breakdown analysis also shows that CMTM3 promoter methylation is associated with cancer risk among patients with stage I LSCC (P<0.001).
  
  
  CONCLUSIONS
  In conclusion, our study indicates that elevated CMTM3 methylation is a risk factor in male LSCC patients, especially in the patients with age over 55years and with smoking behavior.
authors: Department of Otorhinolaryngology (Head and Neck Surgery), Ningbo Medical Center Lihuili Hospital, Ningbo, Zhejiang 315040, China. Electronic address: szs7216@163.com.
authors: Zhisen
authors: Z
authors: Shen
authors: Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors: Xiaoying
authors: X
authors: Chen
authors: Department of Otorhinolaryngology (Head and Neck Surgery), Ningbo Medical Center Lihuili Hospital, Ningbo, Zhejiang 315040, China.
authors: Qun
authors: Q
authors: Li
authors: Department of Otorhinolaryngology (Head and Neck Surgery), Ningbo Medical Center Lihuili Hospital, Ningbo, Zhejiang 315040, China; Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors: Chongchang
authors: C
authors: Zhou
authors: Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors: Yan
authors: Y
authors: Xu
authors: Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors: Rui
authors: R
authors: Yu
authors: Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors: Huadan
authors: H
authors: Ye
authors: Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors: Jinyun
authors: J
authors: Li
authors: Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China. Electronic address: duanshiwei@nbu.edu.cn.
authors: Shiwei
authors: S
authors: Duan
mesh_terms: D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D018925:Chemokines / Q000235:genetics*; D019175:DNA Methylation; D057809:HEK293 Cells; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D062792:MARVEL Domain-Containing Proteins / Q000235:genetics*; D008297:Male; D011401:Promoter Regions, Genetic
chemical_list: C515016:CMTM3 protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins
keywords: CMTM3; DNA methylation; Laryngeal carcinoma; Male; Promoter