title: Anthocyanins degradation during storage of Hibiscus sabdariffa extract and evolution of its degradation products.
abstract: Degradation parameters of two main anthocyanins from roselle extract (Hibiscus sabdariffa L.) stored at different temperatures (4-37°C) over 60days were determined. Anthocyanins and some of their degradation products were monitored and quantified using HPLC-MS and DAD. Degradation of anthocyanins followed first-order kinetics and reaction rate constants (k values), which were obtained by non-linear regression, showed that the degradation rate of delphinidin 3-O-sambubioside was higher than that of cyanidin 3-O-sambubioside with k values of 9.2·10(-7)s(-1) and 8.4·10(-7)s(-1) at 37°C respectively. The temperature dependence of the rate of anthocyanin degradation was modeled by the Arrhenius equation. Degradation of delphinidin 3-O-sambubioside (Ea=90kJmol(-1)) tended to be significantly more sensitive to an increase in temperature than cyanidin 3-O-sambubioside (Ea=80kJmol(-1)). Degradation of these anthocyanins formed scission products (gallic and protocatechuic acids respectively) and was accompanied by an increase in polymeric color index.
authors: Instituto Superior de Tecnologia Agro-alimentar de Malanje (ISTAM), EN 230 km 2, Malanje, Angola. Electronic address: andre_sinela@yahoo.com.br.
authors: André
authors: A
authors: Sinela
authors: Centre International de Recherche Agronomique pour le Développement (CIRAD), UMR95 Qualisud, TA B-95/16, 73 rue J.F. BretonF-34398, Montpellier Cedex 5, France. Electronic address: nadirah.rawat@gmail.com.
authors: Nadirah
authors: N
authors: Rawat
authors: Centre International de Recherche Agronomique pour le Développement (CIRAD), UMR95 Qualisud, TA B-95/16, 73 rue J.F. BretonF-34398, Montpellier Cedex 5, France. Electronic address: christian.mertz@cirad.fr.
authors: Christian
authors: C
authors: Mertz
authors: Montpellier SupAgro, UMR95 QualiSud, F-34093 Montpellier, France. Electronic address: nawel.achir@supagro.inra.fr.
authors: Nawel
authors: N
authors: Achir
authors: Institut National de la Recherche Agronomique (INRA), UMR1083 SPO, F-34060 Montpellier, France. Electronic address: fulcrand@supagro.inra.fr.
authors: Hélène
authors: H
authors: Fulcrand
authors: Montpellier SupAgro, UMR95 QualiSud, F-34093 Montpellier, France. Electronic address: manuel.dornier@cirad.fr.
authors: Manuel
authors: M
authors: Dornier
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D002853:Chromatography, Liquid; D004187:Disaccharides; D031584:Hibiscus / Q000737:chemistry*; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D004187:Disaccharides; D010936:Plant Extracts; C505011:delphinidin 3-sambubioside; C546691:cyanidin 3-sambubioside
keywords: Cyanidin 3-O-sambubioside; Delphinidin 3-O-sambubioside; Hibiscus sabdariffa; Kinetics; Thermal degradation