title: Annexin A10 is involved in the development and maintenance of neuropathic pain in mice.
abstract: ANXA10 (annexin A10) is a member of the annexin family, and its biological effects are mediated primarily through the calcium-dependent phospholipid-binding and calcium ion binding. We examined the gene expressions of the L5 spinal cord after spinal nerve ligation (SNL)-induced neuropathic pain in mice by gene chip. The results showed that Anxa10 mRNA was the most upregulated gene in annexin family with 73.7-fold increase. Although previous studies have reported that several annexins are involved in nociceptive pain, the role of Anxa10 in pain remains undefined. We aimed to evaluate the role of ANXA10 in mediating injury-induced heat hyperalgesia and mechanical allodynia. We found that SNL induced persistent upregulation of Anxa10 mRNA and protein in the spinal cord of mice. Moreover, ANXA10 was colocalized with the neuronal marker MAP2 and astrocytic marker glial fibrillary acidic protein (GFAP), but not with microglial marker CD11b. Finally, pretreatment with Anxa10 siRNA partially prevented SNL-induced mechanical allodynia and heat hyperalgesia. Posttreatment with Anxa10 siRNA attenuated SNL-induced neuropathic pain. These findings suggest that ANXA10 might be a novel target in the treatment of neuropathic pain.
authors: Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Jiangsu 226001, China; Pain Research Laboratory, Institute of Nautical Medicine, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China.
authors: Ying
authors: Y
authors: Lu
authors: Department of Medical Oncology, The affiliated Hospital of Nantong University, Jiangsu 226001, China.
authors: Sujie
authors: S
authors: Ni
authors: Pain Research Laboratory, Institute of Nautical Medicine, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China.
authors: Li-Na
authors: LN
authors: He
authors: Pain Research Laboratory, Institute of Nautical Medicine, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China.
authors: Yong-Jing
authors: YJ
authors: Gao
authors: Pain Research Laboratory, Institute of Nautical Medicine, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China. Electronic address: jiangbaochun@ntu.edu.cn.
authors: Bao-Chun
authors: BC
authors: Jiang
mesh_terms: D000818:Animals; D017302:Annexins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001253:Astrocytes / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D006930:Hyperalgesia / Q000235:genetics / Q000378:metabolism; D008026:Ligation; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009437:Neuralgia / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism*; D013127:Spinal Nerves / Q000293:injuries; D015854:Up-Regulation
chemical_list: D017302:Annexins; C485665:Anxa10 protein, mouse; D012333:RNA, Messenger
keywords: Anxa10; Mice; Neuropathic pain; Spinal cord; Spinal nerve ligation