title: Calcium phosphate-polymer hybrid nanoparticles for enhanced triple negative breast cancer treatment via co-delivery of paclitaxel and miR-221/222 inhibitors.
abstract: In this study, a development of a novel calcium phosphate-polymer hybrid nanoparticle system is reported.The nanoparticle system can co-encapsulate and co-deliver a combination of therapeutic agents with different physicochemical properties (i.e., inhibitors for microRNA-221 and microRNA-222 (miRi-221/222) and paclitaxel (pac)).miRi-221/222 are hydrophilic and were encapsulated with calcium phosphate by co-precipitation in a water-in-oil emulsion.The precipitates were then coated with an anionic lipid, dioleoylphosphatidic acid (DOPA), to co-encapsulate hydrophobic paclitaxel outside the hydrophilic precipitates and inside the same nanoparticle.The nanoparticles formed by following this approach had a size of about ≤100nm and contained both lipid-coated calcium phosphate/miRi and paclitaxel.This nanoparticle system was found to simultaneously deliver paclitaxel and miRi-221/222 to their intracellular targets, leading to inhibit proliferative mechanisms of miR-221/222 and thus significantly enhancing the therapeutic efficacy of paclitaxel.
authors: Chemical Engineering Program, Department of Biomedical, Environmental, and Chemical Engineering, University of Cincinnati, Cincinnati, OH, USA.
authors: Zilan
authors: Z
authors: Zhou
authors: Chemical Engineering Program, Department of Biomedical, Environmental, and Chemical Engineering, University of Cincinnati, Cincinnati, OH, USA.
authors: Carly
authors: C
authors: Kennell
authors: Chemical Engineering Program, Department of Biomedical, Environmental, and Chemical Engineering, University of Cincinnati, Cincinnati, OH, USA. Electronic address: joo.lee@uc.edu.
authors: Joo-Youp
authors: JY
authors: Lee
authors: Department of Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, OH, USA.
authors: Yuet-Kin
authors: YK
authors: Leung
authors: Department of Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, OH, USA.
authors: Pheruza
authors: P
authors: Tarapore
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage*; D002130:Calcium Phosphates; D045744:Cell Line, Tumor; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors; D053758:Nanoparticles; D017239:Paclitaxel / Q000008:administration & dosage*; D011108:Polymers; D064726:Triple Negative Breast Neoplasms / Q000188:drug therapy*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002130:Calcium Phosphates; D035683:MicroRNAs; D017239:Paclitaxel; D011108:Polymers; C525709:MIR221, human; C525710:MIR222, human
keywords: Cancer therapy; Co-delivery; Polymeric nanoparticle; RNA interference; Synergistic effect