title: Genome-Wide Association Study Provides Insight into the Genetic Control of Plant Height in Rapeseed (Brassica napus L.).
abstract: Plant height is a key morphological trait of rapeseed. In this study, we measured plant height of a rapeseed population across six environments. This population contains 476 inbred lines representing the major Chinese rapeseed genepool and 44 lines from other countries. The 60K Brassica Infinium® SNP array was utilized to genotype the association panel. A genome-wide association study (GWAS) was performed via three methods, including a robust, novel, nonparametric Anderson-Darling (A-D) test. Consequently, 68 loci were identified as significantly associated with plant height (P < 5.22 × 10(-5)), and more than 70% of the loci (48) overlapped the confidence intervals of reported QTLs from nine mapping populations. Moreover, 24 GWAS loci were detected with selective sweep signals, which reflected the signatures of historical semi-dwarf breeding. In the linkage disequilibrium (LD) decay range up-and downstream of 65 loci (r (2) > 0.1), we found plausible candidates orthologous to the documented Arabidopsis genes involved in height regulation. One significant association found by GWAS colocalized with the established height locus BnRGA in rapeseed. Our results provide insights into the genetic basis of plant height in rapeseed and may facilitate marker-based breeding.
authors: National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.
authors: Chengming
authors: C
authors: Sun
authors: National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.
authors: Benqi
authors: B
authors: Wang
authors: National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.
authors: Lei
authors: L
authors: Yan
authors: National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.
authors: Kaining
authors: K
authors: Hu
authors: National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.
authors: Sheng
authors: S
authors: Liu
authors: National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.
authors: Yongming
authors: Y
authors: Zhou
authors: Southern Regional Collaborative Innovation Center for Grain and Oil Crops, College of Agronomy, Hunan Agricultural University Changsha, China.
authors: Chunyun
authors: C
authors: Guan
authors: Southern Regional Collaborative Innovation Center for Grain and Oil Crops, College of Agronomy, Hunan Agricultural University Changsha, China.
authors: Zhenqian
authors: Z
authors: Zhang
authors: Chongqing Rapeseed Engineering Technology Research Center, College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors: Jiana
authors: J
authors: Li
authors: Key Laboratory of Cotton and Rapeseed, Jiangsu Academy of Agricultural Science Nanjing, China.
authors: Jiefu
authors: J
authors: Zhang
authors: Key Laboratory of Cotton and Rapeseed, Jiangsu Academy of Agricultural Science Nanjing, China.
authors: Song
authors: S
authors: Chen
authors: National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.
authors: Jing
authors: J
authors: Wen
authors: National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.
authors: Chaozhi
authors: C
authors: Ma
authors: National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.
authors: Jinxing
authors: J
authors: Tu
authors: National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.
authors: Jinxiong
authors: J
authors: Shen
authors: National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.
authors: Tingdong
authors: T
authors: Fu
authors: National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.
authors: Bin
authors: B
authors: Yi
keywords: Brassica napus; association mapping; haplotype block; plant height; selective sweep