title: Evaluation of Mitochondrial Respiratory Chain on the Generation of Reactive Oxygen Species and Cytotoxicity in HaCaT Cells Induced by Nanosized Titanium Dioxide Under UVA Irradiation.
abstract: Nanosized titanium dioxide (nano-TiO2) is widely used in the chemical, electrical, and electronic industries. Nanosized TiO2has been reported to be an efficient photocatalyst, which is able to produce reactive oxygen species (ROS) under UVA irradiation. In the present work, we evaluate the effect of mitochondrial respiratory chain on the generation of ROS and cytotoxicity in keratinocyte (HaCaT) cells induced by nano-TiO2under UVA irradiation. HaCaT cells were pretreated with different inhibitors of mitochondrial respiratory chain and followed by treatment with 200 µg/mL nano-TiO2, then exposed to UVA (365 nm) for 1 hour and cultured for 24 hours. Our results demonstrated that the complexes I and III of the mitochondrial respiratory chain are the major site in the ROS generation induced by nano-TiO2Our results also demonstrated that the uncouplers of mitochondrial oxidative phosphorylation resulted in obvious changes in the production of intracellular ROS induced by nano-TiO2The ROS sources of lipoxygenase, cyclooxygenase, and nicotinamide adenine dinucleotide phosphate oxidase had no significant effect on the ROS production. To some extent, nitric oxide synthase had effect on the ROS production. These results indicated that mitochondrial respiratory chain may be the main source of intracellular ROS production induced by nano-TiO2.
authors: University Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, China.|College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors: Chengbin
authors: C
authors: Xue
authors: University Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, China 493303104@qq.com.
authors: Xiaonan
authors: X
authors: Li
authors: University Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors: Guozhen
authors: G
authors: Liu
authors: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors: Wei
authors: W
authors: Liu
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D004579:Electron Transport / Q000187:drug effects; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D053768:Metal Nanoparticles / Q000528:radiation effects / Q000633:toxicity*; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014025:Titanium / Q000528:radiation effects / Q000633:toxicity*; D014466:Ultraviolet Rays
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D017382:Reactive Oxygen Species; C009495:titanium dioxide; D008315:Malondialdehyde; D045304:Cytochromes c; D014025:Titanium
keywords: HaCaT cells; UVA irradiation.; mitochondrial respiratory chain; nanosized titanium dioxide; reactive oxygen species