title: miRNA profiles in livers with different mass deficits after partial hepatectomy and miR-106b~25 cluster accelerating hepatocyte proliferation in rats.
abstract: Partial hepatectomy (PH) promotes the reentry of quiescent hepatocytes into cell cycle for regrowth. miRNA profiles in livers with different mass deficits after PH have not been investigated and miRNAs implicated in liver regeneration remain unclear. We generated miRNA profiles from normal and remnant livers at 6, 12, 24, and 36 hours after 1/3 or 2/3PH using microarrays. Compared with normal livers, the proportion of altered miRNAs decreased with time after 1/3PH, but increased after 2/3PH. Most of altered miRNAs between 1/3 and 2/3PH exhibited similar up- or down-regulation, but lower expression magnitude for 1/3PH. Among differentially expressed miRNAs between 2/3PH with robust DNA replication and 1/3PH with a minimal replicative response, we identified miR-101a, miR-92a, miR-25, miR-93 and miR-106b as key regulators of cell cycle. In 2/3PH model, overexpression of miR-106b~25 cluster tended to accelerate liver regeneration, while inhibition of miR-106b~25 cluster markedly repressed regenerative response and delayed recovery of liver function. Mechanistically, RB1 and KAT2B with cell cycle arrest activity were identified as novel targets of miR-106b/93 and miR-25, respectively. Overall, we featured miRNA profiles and dynamics after 1/3 and 2/3PH, and identified miR-106b~25 cluster as being involved in timely cell cycle entry of hepatocytes after PH.
authors: Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: Xiao
authors: X
authors: Xu
authors: Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: Zhikun
authors: Z
authors: Liu
authors: Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, China.
authors: Jianguo
authors: J
authors: Wang
authors: Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: Qi
authors: Q
authors: Ling
authors: Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, China.
authors: Haiyang
authors: H
authors: Xie
authors: Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, China.
authors: Haijun
authors: H
authors: Guo
authors: Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, China.
authors: Xuyong
authors: X
authors: Wei
authors: Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, China.
authors: Lin
authors: L
authors: Zhou
authors: Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: Shusen
authors: S
authors: Zheng
mesh_terms: D000818:Animals; D002453:Cell Cycle; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D057809:HEK293 Cells; D006498:Hepatectomy; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000601:surgery; D008115:Liver Regeneration; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C000621081:MicroRNA-106b-5p, rat; D035683:MicroRNAs