title: Estrogen receptor-α, progesterone receptor, and c-erbB/HER-family receptor mRNA detection and phenotype analysis in spontaneous canine models of breast cancer.
abstract: Well characterized, stable, p16-defective canine mammary cancer (CMT) cell lines and normal canine mammary epithelial cells were used to investigate expression of the major breast cancer-specific hormone receptors estrogen receptor alpha (ER1) and progesterone receptor (PR) as well as luminal epithelial-specific proto-oncogenes encoding c-erbB-1 (epidermal growth factor receptor/EGFr), c-erbB-2/HER2, c-erbB-3, and c-erbB-4 receptors. The investigation developed and validated quantitative reverse transcriptase polymerase chain reaction assays for each transcript to provide rapid assessment of breast cancer phenotypes for canine cancers, based on ER1, PR, and c-erbB-2/HER2 expressions, similar to those in human disease. Roles for relatively underexplored c-erbB-3 and c-erbB-4 receptor expressions in each of these breast cancer phenotypes were also evaluated. Each quantitative assay was validated by assessment of amplicon size and DNA sequencing following amplification. Differential expression of ER1, PR, and c-erbB-2 in CMT cell lines clearly defined distinct human-like breast cancer phenotypes for a selection of CMT-derived cell lines. Expression profiles for EGFr family genes c-erbB-3 and c-erbB-4 in CMT models also provided an enriched classification of canine breast cancer identifying new extended phenotypes beyond the conventional luminal-basal characterization used in human breast cancer.
authors: Auburn University Research Initiative in Cancer (AURIC), Department of Pathobiology, College of Veterinary Medicine, Harrison School of Pharmacy, Auburn University, AL 36849, USA.
authors: Farruk M Lutful
authors: FML
authors: Kabir
authors: Auburn University Research Initiative in Cancer (AURIC), Department of Pathobiology, College of Veterinary Medicine, Harrison School of Pharmacy, Auburn University, AL 36849, USA.
authors: Patricia
authors: P
authors: DeInnocentes
authors: Scott-Ritchey Research Center, College of Veterinary Medicine, Harrison School of Pharmacy, Auburn University, AL 36849, USA.
authors: Payal
authors: P
authors: Agarwal
authors: Department of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, AL 36849, USA.
authors: Christopher P
authors: CP
authors: Mill
authors: Department of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, AL 36849, USA.
authors: David J
authors: DJ
authors: Riese Nd
authors: Auburn University Research Initiative in Cancer (AURIC), Department of Pathobiology, College of Veterinary Medicine, Harrison School of Pharmacy, Auburn University, AL 36849, USA.
authors: R Curtis
authors: RC
authors: Bird
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D066246:ErbB Receptors / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005260:Female; D015674:Mammary Neoplasms, Animal / Q000378:metabolism*; D010641:Phenotype; D011980:Receptors, Progesterone / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D047628:Estrogen Receptor alpha; D011980:Receptors, Progesterone; D066246:ErbB Receptors
keywords: breast cancer; c-erbB/HER-2 receptor; canine; estrogen/progesterone receptor; mammary epithelial cells