title: Target-controlled gating liposome "off-on" cascade amplification for sensitive and accurate detection of phospholipase D in breast cancer cells with a low-background signal.
abstract: Here we developed a simple, sensitive and accurate PLD detection method based on a target-controlled gating liposome (TCGL) "off-on" cascade amplified strategy and personal glucose meters (PGMs). It showed excellent sensitivity with a detection limit of 0.005 U L(-1) and well performed PLD activity analysis in breast cancer cells and inhibitor drug screening.
authors: School of Pharmacy, Shandong University, Jinan 250012, P. R. China. wangl-sdu@sdu.edu.cn and Department of Chemistry, Liaocheng University, Liaocheng, 252059, Shandong, China.
authors: Qingwang
authors: Q
authors: Xue
authors: School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China. wjiang@sdu.edu.cn.
authors: Wei
authors: W
authors: Jiang
authors: School of Pharmacy, Shandong University, Jinan 250012, P. R. China. wangl-sdu@sdu.edu.cn.
authors: Lei
authors: L
authors: Wang
mesh_terms: D000688:Amylose / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation*; D001943:Breast Neoplasms / Q000201:enzymology*; D045744:Cell Line, Tumor; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation; D057075:Enzyme Assays / Q000295:instrumentation*; D005260:Female; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D006801:Humans; D057230:Limit of Detection; D008081:Liposomes / Q000378:metabolism / Q000648:ultrastructure; D010739:Phospholipase D / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D008081:Liposomes; D000688:Amylose; D010739:Phospholipase D; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose