title: Cobalt(II) complexes of sparfloxacin: Characterization, structure, antimicrobial activity and interaction with DNA and albumins.
abstract: The cobalt(II) complexes with the quinolone sparfloxacin (Hsf) in the absence or presence of the nitrogen-donor heterocyclic ligands 2,2'-bipyridine (bipy), 1,10-phenanthroline (phen) or 2,2'-bipyridylamine (bipyam) were prepared and characterized physicochemically and spectroscopically. The crystal structures of complexes [Co(sf)2(bipy)]∙3MeOH∙2H2O and [Co(sf)2(phen)]∙4MeOH were determined by X-ray crystallography. The antimicrobial activity of the complexes was tested against four different microorganisms (Escherichia coli, Xanthomonas campestris, Staphylococcus aureus and Bacillus subtilis) and was found similar or higher than that of free Hsf. The binding of the complexes to calf-thymus DNA was monitored by UV-vis spectroscopy and DNA-viscosity measurements and indirectly by competitive studies with ethidium bromide; intercalation is suggested as the most possible interaction mode. Fluorescence emission spectroscopy was used to evaluate the interaction of the complexes with human or bovine serum albumin and the corresponding binding constants were determined.
authors: Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
authors: Eleftherios
authors: E
authors: Kouris
authors: Department of Nutrition and Dietetics, Faculty of Agriculture, Food Technology and Nutrition, Alexander Technological Educational Institution, Sindos, Thessaloniki, Greece.
authors: Stavros
authors: S
authors: Kalogiannis
authors: Faculty of Chemistry and Chemical Technology, University of Ljubljana, Vecna pot 113, 1000 Ljubljana, Slovenia.
authors: Franc
authors: F
authors: Perdih
authors: Faculty of Chemistry and Chemical Technology, University of Ljubljana, Vecna pot 113, 1000 Ljubljana, Slovenia.
authors: Iztok
authors: I
authors: Turel
authors: Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece. Electronic address: gepsomas@chem.auth.gr.
authors: George
authors: G
authors: Psomas
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000254:growth & development*; D002417:Cattle; D003035:Cobalt / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000737:chemistry*; D024841:Fluoroquinolones / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D015394:Molecular Structure; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D012710:Serum Albumin, Bovine; D003035:Cobalt; D004247:DNA; C427203:calf thymus DNA; C061363:sparfloxacin
keywords: Antimicrobial activity; Cobalt complexes; Interaction with DNA; Interaction with albumins; Sparfloxacin