title: Oxidative Stress Assessment in Response to Ultraendurance Exercise: Thiols Redox Status and ROS Production according to Duration of a Competitive Race.
abstract: Purpose. Response to an ultraendurance competitive race on thiols redox status, reactive oxygen species (ROS) production, and oxidative stress (OxS) was investigated according to duration. Methods. Twenty-four elite runners were examined: six completed 50 km and eighteen 100 km. Blood and urine samples were collected before and immediately after the race. Erythrocytes and plasma aminothiols by high-performance liquid chromatography, total antioxidant capacity (TAC), and OxS biomarkers (protein carbonyl (PC), thiobarbituric acid-reactive substances (TBARS), 8-isoprostane (8-iso-PGF2α), and 8-OH-2-deoxyguanosine (8-OH-dG)) by immunoenzymatic assays and ROS production by Electron Paramagnetic Resonance were assessed. Results. Significant increases (P between <0.05 and <0.0001) were recorded in plasma total and oxidized aminothiols concentration and TAC (P < 0.0001) only after 100 km: plasmatic (ROS production (+12 versus +29%), PC (+54 versus +115%), and TBARS (+28 versus +55%)) and urinary (8-OH-dG.creatinine(-1) (+71 versus +158%) and 8-iso-PGF2α.creatinine(-1) (+43 versus +135%)) concentrations for 50 and 100 km (duration 4 h 3' versus 8 h 42'), respectively. Conclusion. Very prolonged ultraendurance exercise causes an increase in ROS production and OxS depending on specific biomarker examined but always linearly and directly related to exercise duration. Redox status of erythrocytes was preserved. A relationship between running performance and both prerace ROS production and antioxidant-redox status was found in 100 km race.
authors: Institute of Bioimaging and Molecular Physiology, National Research Council (CNR), Via Fratelli Cervi 93, 20090 Segrate, Italy.
authors: Alessandra
authors: A
authors: Vezzoli
authors: Institute of Clinical Physiology, National Research Council (CNR), Niguarda Ca' Granda Hospital, Via G. Moruzzi 1, 56124 Pisa, Italy.
authors: Cinzia
authors: C
authors: Dellanoce
authors: Institute of Bioimaging and Molecular Physiology, National Research Council (CNR), Via Fratelli Cervi 93, 20090 Segrate, Italy.
authors: Simona
authors: 0000-0001-7359-2690
authors: S
authors: Mrakic-Sposta
authors: Institute of Bioimaging and Molecular Physiology, National Research Council (CNR), Via Fratelli Cervi 93, 20090 Segrate, Italy; Telematic University San Raffaele, Via F. Daverio 7, 20122 Milan, Italy.
authors: Michela
authors: M
authors: Montorsi
authors: Institute of Bioimaging and Molecular Physiology, National Research Council (CNR), Via Fratelli Cervi 93, 20090 Segrate, Italy.
authors: Sarah
authors: 0000-0002-9208-017X
authors: S
authors: Moretti
authors: Institute of Clinical Physiology, National Research Council (CNR), Niguarda Ca' Granda Hospital, Via G. Moruzzi 1, 56124 Pisa, Italy.
authors: Annamaria
authors: A
authors: Tonini
authors: Institute of Clinical Physiology, National Research Council (CNR), Niguarda Ca' Granda Hospital, Via G. Moruzzi 1, 56124 Pisa, Italy.
authors: Lorenza
authors: 0000-0002-1758-5485
authors: L
authors: Pratali
authors: Institute of Clinical Physiology, National Research Council (CNR), Niguarda Ca' Granda Hospital, Via G. Moruzzi 1, 56124 Pisa, Italy.
authors: Roberto
authors: R
authors: Accinni
mesh_terms: D000328:Adult; D000886:Anthropometry; D056352:Athletes; D015415:Biomarkers / Q000097:blood / Q000652:urine; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010807:Physical Endurance / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000097:blood*; D012420:Running / Q000502:physiology*; D013438:Sulfhydryl Compounds / Q000097:blood*
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds