title: Polyvinylpolypyrrolidone reduces cross-reactions between antibodies and phenolic compounds in an enzyme-linked immunosorbent assay for the detection of ochratoxin A.
abstract: Ochratoxin A (OTA) is a fungal metabolite and putative carcinogen which can contaminate a variety of foods such as cereals, wine, and nuts. Commercial ELISA kits are known to give false-positive results for OTA concentrations when phenolic compounds are present. Pistachios represent a food matrix rich in phenolic compounds potentially contaminated with OTA, and were used to model OTA cross-reactivity. Polyvinylpolypyrrolidone (PVPP) was incorporated during extraction of OTA using a commercial ELISA protocol. HPLC methods were used to confirm that PVPP does not interact with OTA and levels of gallic acid and catechin remaining in pistachio extracts decreased with increasing PVPP application. Cross-reactivity of extracts also decreased with increasing PVPP application, and color loss was used as an indicator of anthocyanin removal. Incorporating PVPP into ELISA protocols allows for the continued use of rapid immunological methods in food matrices containing phenolic compounds.
authors: School of Food Science, University of Idaho, 875 Perimeter Drive MS 2312, Moscow, Idaho 83844-2312, USA.
authors: Andrew L
authors: AL
authors: Robinson
authors: School of Food Science, University of Idaho, 875 Perimeter Drive MS 2312, Moscow, Idaho 83844-2312, USA.
authors: Hyun Jung
authors: HJ
authors: Lee
authors: School of Food Science, University of Idaho, 875 Perimeter Drive MS 2312, Moscow, Idaho 83844-2312, USA. Electronic address: dryu@uidaho.edu.
authors: Dojin
authors: D
authors: Ryu
mesh_terms: D000906:Antibodies / Q000032:analysis / Q000378:metabolism; D002392:Catechin / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003429:Cross Reactions / Q000187:drug effects; D002523:Edible Grain / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D009793:Ochratoxins / Q000032:analysis* / Q000378:metabolism; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D027927:Pistacia; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D011205:Povidone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000906:Antibodies; D009793:Ochratoxins; D010636:Phenols; D010936:Plant Extracts; C025589:ochratoxin A; C077842:polyvinylpolypyrrolidone; D002392:Catechin; D011205:Povidone
keywords: Cross-reactivity; ELISA; Ochratoxin A (OTA); Phenolic compounds; Polyvinylpolypyrrolidone (PVPP)