title: Detection of Cronobacter Genus in Powdered Infant Formula by Enzyme-linked Immunosorbent Assay Using Anti-Cronobacter Antibody.
abstract: Cronobacter species (Cronobacter spp.) are hazardous foodborne pathogens associated with baby food, powdered infant formula (PIF). To develop a rapid and sensitive method for simultaneous detection of seven Cronobacter spp. in PIF, an indirect non-competitive enzyme-linked immunosorbent assay (INC-ELISA) was developed based on a novel immunoglobulin G (IgG), anti-Cronobacter IgG. The developed INC-ELISA was able to detect seven Cronobacter spp. at concentrations ranging from (5.6 ± 0.30) × 10(3) to (2.1 ± 0.01) × 10(5) colony forming unit (CFU)/mL in pure culture. Further, INC-ELISA employing anti-Cronobacter IgG was applicable for analysis of PIF samples contaminated with less than <10 cells of Cronobacter spp. per 25 g of PIF in 36 h. The developed antibody showed slight cross-reactivity with Franconibacter pulveris (LMG 24057) at high concentration (10(8) CFU/mL). The INC-ELISA method displayed excellent specificity without compromising cross-reactivity with other foodborne pathogens. The INC-ELISA assay method developed in this study using a novel anti-Cronobacter IgG facilitated highly sensitive, efficient, and rapid detection of Cronobacter spp. in baby food.
authors: Department of Food Science and Technology, Yeungnam University Gyeongsan, South Korea.
authors: Xinjie
authors: X
authors: Song
authors: Department of Food Science and Technology, Yeungnam University Gyeongsan, South Korea.
authors: Shruti
authors: S
authors: Shukla
authors: Department of Food Science and Technology, Yeungnam University Gyeongsan, South Korea.
authors: Gibaek
authors: G
authors: Lee
authors: Food Standard Research Center, Korea Food Research Institute Seongnam, South Korea.
authors: Sunhyun
authors: S
authors: Park
authors: Department of Food Science and Technology, Yeungnam University Gyeongsan, South Korea.
authors: Myunghee
authors: M
authors: Kim
keywords: Cronobacter species; genus-specificity; immunoglobulin G; indirect non-competitive enzyme-linked immunosorbent assay; powdered infant formula