title: Photo-biomodulatory response of low-power laser irradiation on burn tissue repair in mice.
abstract: The present work reports the photo-biomodulatory effect of red (632.8 nm) and near infrared (785 and 830 nm) lasers on burn injury in Swiss albino mice. Animals were induced with a 15-mm full thickness burn injury and irradiated with various fluences (1, 2, 3, 4, and 6 J/cm2) of each laser wavelength under study having a constant fluence rate (8.49 mW/cm2). The size of the injury following treatment was monitored by capturing the wound images at regular time intervals until complete healing. Morphometric assessment indicated that the group treated with 3-J/cm2fluence of 830 nm had a profound effect on healing as compared to untreated controls and various fluences of other wavelengths under study. Histopathological assessment of wound repair on treatment with an optimum fluence (3 J/cm2) of 830 nm performed on days 2, 6, 12, and 18 post-wounding resulted in enhanced wound repair with migration of fibroblasts, deposition of collagen, and neovascularization as compared to untreated controls. The findings of the present study have clearly demonstrated that a single exposure of 3-J/cm2fluence at 830-nm enhanced burn wound healing progression in mice, which is equivalent to 5 % povidone iodine treatment (reference standard), applied on a daily basis till complete healing.
authors: Department of Biophysics, School of Life Sciences, Manipal University, Manipal, 576104, India.
authors: Bharath
authors: B
authors: Rathnakar
authors: Department of Radiation Biology and Toxicology, School of Life Sciences, Manipal University, Manipal, 576104, India.
authors: Bola Sadashiva Satish
authors: BS
authors: Rao
authors: Department of Biophysics, School of Life Sciences, Manipal University, Manipal, 576104, India.|Department of Biotechnology, MIT, Manipal University, Manipal, 576104, India.
authors: Vijendra
authors: V
authors: Prabhu
authors: Department of Biophysics, School of Life Sciences, Manipal University, Manipal, 576104, India.
authors: Subhash
authors: S
authors: Chandra
authors: Department of Pathology, KMC Mangalore, Manipal University, Mangalore, 576104, India.
authors: Sharada
authors: S
authors: Rai
authors: Department of Pathology, KMC Manipal, Manipal University, Manipal, 576104, India.|Department of Pathology, Srinivas Institute of Medical Sciences and Research Centre, Surathkal, Mangalore, 575021, India.
authors: Anuradha Calicut Kini
authors: AC
authors: Rao
authors: Laser Biomedical Applications and Instrumentation Division, RRCAT, Indore, 452013, India.
authors: Mrinalini
authors: M
authors: Sharma
authors: Laser Biomedical Applications and Instrumentation Division, RRCAT, Indore, 452013, India.
authors: Pradeep Kumar
authors: PK
authors: Gupta
authors: Department of Biophysics, School of Life Sciences, Manipal University, Manipal, 576104, India. mahato.kk@manipal.edu.
authors: Krishna Kishore
authors: KK
authors: Mahato
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology / Q000532:radiotherapy*; D003094:Collagen; D005260:Female; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D051379:Mice; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D003094:Collagen
keywords: Burns; Histopathology; Low-level light therapy; Wound healing