title: Development and validation of an ultra-performance convergence chromatography method for the quality control of Angelica gigas Nakai.
abstract: Ultra-performance convergence chromatography, which integrates the advantages of supercritical fluid chromatography and ultra high performance liquid chromatography technologies, is an environmentally friendly analytical method that uses dramatically reduced amounts of organic solvents. An ultra-performance convergence chromatography method was developed and validated for the quantification of decursinol angelate and decursin in Angelica gigas using a CSH Fluoro-Phenyl column (2.1 mm × 150 mm, 1.7 μm) with a run time of 4 min. The method had an improved resolution and a shorter analysis time in comparison to the conventional high-performance liquid chromatography method. This method was validated in terms of linearity, precision, and accuracy. The limits of detection were 0.005 and 0.004 μg/mL for decursinol angelate and decursin, respectively, while the limits of quantitation were 0.014 and 0.012 μg/mL, respectively. The two components showed good regression (correlation coefficient (r2) > 0.999), excellent precision (RSD < 2.28%), and acceptable recoveries (99.75-102.62%). The proposed method can be used to efficiently separate, characterize, and quantify decursinol angelate and decursin in Angelica gigas and its related medicinal materials or preparations, with the advantages of a shorter analysis time, greater sensitivity, and better environmental compatibility.
authors: K-Herb Research Center, Korea Institute of Oriental Medicine, Yuseong-gu, Daejeon, Republic of Korea.
authors: Hyo Seon
authors: HS
authors: Kim
authors: K-Herb Research Center, Korea Institute of Oriental Medicine, Yuseong-gu, Daejeon, Republic of Korea.
authors: Jin Mi
authors: JM
authors: Chun
authors: K-Herb Research Center, Korea Institute of Oriental Medicine, Yuseong-gu, Daejeon, Republic of Korea.
authors: Bo-In
authors: BI
authors: Kwon
authors: K-Herb Research Center, Korea Institute of Oriental Medicine, Yuseong-gu, Daejeon, Republic of Korea.
authors: A-Reum
authors: AR
authors: Lee
authors: Mibyeong Research Center, Korea Institute of Oriental Medicine, Yuseong-gu, Daejeon, Republic of Korea.
authors: Ho Kyoung
authors: HK
authors: Kim
authors: K-Herb Research Center, Korea Institute of Oriental Medicine, Yuseong-gu, Daejeon, Republic of Korea. lay7709@kiom.re.kr.
authors: A Yeong
authors: AY
authors: Lee
mesh_terms: D029969:Angelica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D010936:Plant Extracts / Q000032:analysis*; D011786:Quality Control
chemical_list: D010936:Plant Extracts
keywords: Angelica gigas Nakai; Green chemistry; Supercritical fluid chromatography; Ultra-performance convergence chromatography