title: Burkholderia thailandensis: Growth and Laboratory Maintenance.
abstract: Burkholderia thailandensis is a nonpathogenic Gram-negative bacterium found in tropical soils. Closely related to several human pathogens, its ease of genetic manipulation, rapid growth in the laboratory, and low virulence make B. thailandensis a commonly used model organism. This unit describes the fundamental protocols for in vitro growth and maintenance of B. thailandensis in the laboratory. © 2016 by John Wiley & Sons, Inc.
authors: Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
authors: Erin C
authors: EC
authors: Garcia
authors: Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
authors: Peggy A
authors: PA
authors: Cotter
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D019117:Burkholderia / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D019121:Burkholderia Infections / Q000382:microbiology; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003470:Culture Media / Q000378:metabolism; D006801:Humans
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media
keywords: Burkholderia pseudomallei; Burkholderia thailandensis; growth; model organism