title: Parainfluenza virus 5 upregulates CD55 expression to produce virions with enhanced resistance to complement-mediated neutralization.
abstract: Many enveloped RNA viruses recruit host cell proteins during assembly as a mechanism to limit antiviral effects of complement. Using viruses which incorporated CD46 alone, CD55 alone or both CD46 and CD55, we addressed the role of these two host cell regulators in limiting complement-mediated neutralization of Parainfluenza virus 5 (PIV5). PIV5 incorporated functional forms of both CD55 and CD46 into virions. PIV5 containing CD55 was highly resistant to complement-mediated neutralization, whereas CD46-containing PIV5 was as sensitive to neutralization as virus lacking both regulators. PIV5 infected cells had increased levels of cell surface CD55, which was further upregulated by exogenous treatment with tumor necrosis factor alpha. PIV5 derived from cells with higher CD55 levels was more resistant to complement-mediated neutralization in vitro than virus from control cells. We propose a role for virus induction of host cell complement inhibitors in defining virus growth and tissue tropism.
authors: Burnett School of Biomedical Sciences, University of Central Florida, College of Medicine, Orlando, FL 32827, USA.
authors: Yujia
authors: Y
authors: Li
authors: Department of Microbiology and Immunology, Wake Forest University, School of Medicine, Winston-Salem, NC 27157-1064, USA.
authors: John B
authors: JB
authors: Johnson
authors: Burnett School of Biomedical Sciences, University of Central Florida, College of Medicine, Orlando, FL 32827, USA; Department of Microbiology and Immunology, Wake Forest University, School of Medicine, Winston-Salem, NC 27157-1064, USA. Electronic address: Griffith.parks@ucf.edu.
authors: Griffith D
authors: GD
authors: Parks
mesh_terms: D000818:Animals; D018958:CD55 Antigens / Q000235:genetics* / Q000378:metabolism; D016466:CHO Cells; D002460:Cell Line; D003167:Complement Activation / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology*; D006224:Cricetinae; D003412:Cricetulus; D005786:Gene Expression Regulation; D006801:Humans; D051916:Membrane Cofactor Protein / Q000235:genetics / Q000378:metabolism; D009500:Neutralization Tests; D045402:Parainfluenza Virus 5 / Q000502:physiology*; D019351:Rubulavirus Infections / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D014779:Virus Replication
chemical_list: D018958:CD55 Antigens; D051916:Membrane Cofactor Protein; D003165:Complement System Proteins
keywords: Complement; Parainfluenza virus