title: Effects of carvedilol on ventricular remodeling and the expression of β3-adrenergic receptor in a diabetic rat model subjected myocardial infarction.
abstract: BACKGROUND
  This study was to assess effects of carvedilol on ventricular remodeling and expression of β3-adrenergic receptor (β3-AR) and Gi protein in a rat model of diabetes subjected to myocardial infarction (MI).
  
  
  METHODS
  Rat model of type II diabetes was established by injection of streptozotion. MI was then induced by ligating the left anterior descending coronary artery. Rats were then randomly divided into two groups treated with either placebo (PL) or carvedilol (CA - 10mg·kg(-1)·d(-)(1)). Additional controls consisted of sham-operated rats with diabetes (DS) and rats fed a normal diet subjected to myocardial infarction (NM). Echocardiographic and hemodynamic studies were performed to assess the structural and functional changes. β3-AR and Gi mRNA in the myocardium distal from the infarction region were measured, and β3-AR and Gi protein were measured with western blot.
  
  
  RESULTS
  There were no significant differences in MI size among the three MI groups. In the PL group, LVEDd, LVWI, E/A and CVF were significantly increased, while LVEF and PW% significantly decreased as compared with the DS and NM groups. Compared with the DS group, the expression of β3-AR and Gi mRNA and protein in the PL group was significantly increased, however, in the CA group, β3-AR and Gi mRNA and protein were decreased.
  
  
  CONCLUSIONS
  The expression of β3-AR and Gi mRNA and protein was increased in diabetic rats subjected to MI as compared with rats subject to either condition alone. Carvedilol treatment prevented many of these deleterious effects.
authors: Cardiovascular Department, The First Affliated Hospital of Harbin Medical University, Harbin 150001, China. Electronic address: zhangruiyingha@126.com.
authors: Ruiying
authors: R
authors: Zhang
authors: Cardiovascular Department, The First Affliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Xiaoning
authors: X
authors: Kang
authors: Cardiovascular Department, The First Affliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Yumei
authors: Y
authors: Wang
authors: Cardiovascular Department, The First Affliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Fei
authors: F
authors: Wang
authors: Cardiovascular Department, The First Affliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Ping
authors: P
authors: Yu
authors: Cardiovascular Department, The First Affliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Jingxia
authors: J
authors: Shen
authors: Cardiovascular Department, The First Affliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Lu
authors: L
authors: Fu
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D002227:Carbazoles / Q000494:pharmacology*; D000077261:Carvedilol; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism*; D004195:Disease Models, Animal; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006334:Heart Function Tests / Q000379:methods; D009203:Myocardial Infarction / Q000150:complications / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism*; D011412:Propanolamines / Q000494:pharmacology*; D051381:Rats; D022702:Receptors, Adrenergic, beta-3 / Q000378:metabolism*; D016896:Treatment Outcome; D020257:Ventricular Remodeling / Q000187:drug effects
chemical_list: D000319:Adrenergic beta-Antagonists; D002227:Carbazoles; D011412:Propanolamines; D022702:Receptors, Adrenergic, beta-3; D000077261:Carvedilol; D019204:GTP-Binding Proteins
keywords: Carvedilol; Gi; Myocardial infarction; Type 2 diabetes; β(3)-AR