title: Identification and expression patterns of novel long non-coding RNAs in neural progenitors of the developing mammalian cortex.
abstract: Long non-coding (lnc)RNAs play key roles in many biological processes. Elucidating the function of lncRNAs in cell type specification during organ development requires knowledge about their expression in individual progenitor types rather than in whole tissues. To achieve this during cortical development, we used a dual-reporter mouse line to isolate coexisting proliferating neural stem cells, differentiating neurogenic progenitors and newborn neurons and assessed the expression of lncRNAs by paired-end, high-throughput sequencing. We identified 379 genomic loci encoding novel lncRNAs and performed a comprehensive assessment of cell-specific expression patterns for all, annotated and novel, lncRNAs described to date. Our study provides a powerful new resource for studying these elusive transcripts during stem cell commitment and neurogenesis.
authors: DFG-Research Center and Cluster of Excellence for Regenerative Therapies; Dresden, Germany; Authors are equal contributing joint-first authors.
authors: Julieta
authors: J
authors: Aprea
authors: Deep Sequencing Group, Biotechnology Center; Dresden, Germany; Authors are equal contributing joint-first authors.
authors: Mathias
authors: M
authors: Lesche
authors: DFG-Research Center and Cluster of Excellence for Regenerative Therapies ; Dresden, Germany.
authors: Simone
authors: S
authors: Massalini
authors: DFG-Research Center and Cluster of Excellence for Regenerative Therapies ; Dresden, Germany.
authors: Silvia
authors: S
authors: Prenninger
authors: Deep Sequencing Group, Biotechnology Center ; Dresden, Germany.
authors: Dimitra
authors: D
authors: Alexopoulou
authors: Deep Sequencing Group, Biotechnology Center ; Dresden, Germany.
authors: Andreas
authors: A
authors: Dahl
authors: Max Planck Institute of Molecular Cell Biology and Genetics; Dresden, Germany; Max Planck Institute for the Physics of Complex Systems; Dresden, Germany.
authors: Michael
authors: M
authors: Hiller
authors: DFG-Research Center and Cluster of Excellence for Regenerative Therapies ; Dresden, Germany.
authors: Federico
authors: F
authors: Calegari
keywords: cortical development; lncRNAs; neurogenesis