title: Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin films.
abstract: Layer-by-Layer (LbL) films containing silk fibroin (SF) and the 40 aminoacid-long amyloid-β peptide (Aβ1-40) were prepared with the purpose of developing a new prototype of an electrochemical immunosensor. The film showed a satisfactory growth in quartz substrate and screen-printed carbon electrodes, as observed by UV-vis spectroscopy and cyclic voltammetric, respectively. The peptide immobilized in LbL films in junction with SF shows secondary structure induced, as shown by circular dichroism measurements, favoring the interaction SF/peptide LbL film with the specific antibody. Immunosensor showed a linear response in the presence of the antibody with concentrations from 0 to 10ngmL(-1) both analyzed by current changes in 0.3V and voltammogram area. This system can be applied as a new prototype for preliminary diagnosis of Alzheimer's disease.
authors: Instituto de Química, Universidade Estadual Paulista "Júlio de Mesquita Filho" UNESP, Rua Professor Francisco Degni, 55, 14800-060 Araraquara, SP, Brazil.
authors: J M
authors: JM
authors: Gonçalves
authors: Instituto de Química, Universidade Estadual Paulista "Júlio de Mesquita Filho" UNESP, Rua Professor Francisco Degni, 55, 14800-060 Araraquara, SP, Brazil.
authors: L R
authors: LR
authors: Lima
authors: Instituto de Ciência e Tecnologia, Universidade Federal de São Paulo UNIFESP, Rua Talim, 330, 12231-280 São José dos Campos, SP, Brazil. Electronic address: marli.moraes@gmail.com.
authors: M L
authors: ML
authors: Moraes
authors: Instituto de Química, Universidade Estadual Paulista "Júlio de Mesquita Filho" UNESP, Rua Professor Francisco Degni, 55, 14800-060 Araraquara, SP, Brazil.
authors: S J L
authors: SJL
authors: Ribeiro
mesh_terms: D000544:Alzheimer Disease / Q000175:diagnosis / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D010446:Peptide Fragments / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D000906:Antibodies; D008567:Membranes, Artificial; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D005349:Fibroins
keywords: Alzheimer's disease; Amyloid-β peptide; Immunosensor; Layer-by-layer; Silk fibroin