title: High-Throughput and High-Content Micronucleus Assay in CHO-K1 Cells.
abstract: Visualization of micronuclei induction by chemicals and drugs enables measurement of possible compound genotoxicity. A loss of entire chromosome or a fragment of chromosome can lead to formation of micronuclei (MNi). The in vitro micronucleus assay can be conducted using nuclear dyes with high-content imaging platforms. This chapter describes the cytochalasin block method of measuring micronuclei in CHO-K1 cell lines.
authors: National Center for Advancing Translational Sciences, National Institutes of Health, Building C, MSC: 3375, 9800 Medical Center Drive, Bethesda, MD, 20892, USA.
authors: Sampada A
authors: SA
authors: Shahane
authors: National Center for Advancing Translational Sciences, National Institutes of Health, Building C, MSC: 3375, 9800 Medical Center Drive, Bethesda, MD, 20892, USA.
authors: Kana
authors: K
authors: Nishihara
authors: National Center for Advancing Translational Sciences, National Institutes of Health, Building C, MSC: 3375, 9800 Medical Center Drive, Bethesda, MD, 20892, USA. mxia@mail.nih.gov.
authors: Menghang
authors: M
authors: Xia
mesh_terms: D000818:Animals; D016466:CHO Cells; D002875:Chromosomes / Q000737:chemistry / Q000187:drug effects*; D003412:Cricetulus; D004249:DNA Damage; D005456:Fluorescent Dyes / Q000737:chemistry; D057166:High-Throughput Screening Assays; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*
chemical_list: D005456:Fluorescent Dyes; D009153:Mutagens
keywords: Genotoxicity; High-content imaging; Micronuclei