title: Preparation and evaluation of immobilized 4-methylbenzoylcellulose stationary phases for enantioselective separations.
abstract: A photochemical method for immobilizing polysaccharide derivatives on silica gel has been developed and applied to 4-methylbenzoyl cellulose (PMBC). The photochemically immobilized materials have been used as chiral stationary phases (CSPs) for the chromatographic separation of the stereoisomers of chiral molecules. Through to the immobilization which makes the chromatographic material insoluble in almost all organic solvents, there no restriction regarding the kind of solvent used in the mobile phase. This feature permits to considerably extend the possibilities to improve the selectivity of the separations and or the solubility of the solute in the mobile phase. The influence of various parameters such as immobilization process, cross-linker type and amount on the chromatographic properties and chiral recognition ability of the resulting CSPs has been investigated using a set of chiral molecules. The impact of the amount of coated polysaccharide material on chiral recognition ability was also examined.
authors: Novartis Pharma AG, Global Discovery Chemistry, 4002 Basel, Switzerland. Electronic address: eric.francotte@novartis.com.
authors: Eric
authors: E
authors: Francotte
authors: Novartis Pharma AG, Global Discovery Chemistry, 4002 Basel, Switzerland.
authors: Tong
authors: T
authors: Zhang
mesh_terms: D002482:Cellulose / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography; D010777:Photochemistry; D011134:Polysaccharides / Q000737:chemistry; D058428:Silica Gel / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D011134:Polysaccharides; D012997:Solvents; D058428:Silica Gel; D002482:Cellulose
keywords: Chiral stationary phases; Enantioselective chromatography; Immobilized polysaccharides