title: Immunomodulatory effects of exposure to polychlorinated biphenyls and perfluoroalkyl acids in East Greenland ringed seals (Pusa hispida).
abstract: To better elucidate the potential immune-related health effects of exposure to environmentally persistent organic pollutants (POP), such as polychlorinated biphenyls (PCBs) and perfluoroalkyl substances (PFASs), in ringed seals (Pusa hispida), a sentinel Arctic species, we assessed 1) associations between mitogen-induced lymphocyte proliferation and in vivo tissue contaminant burdens, and 2) the concentration-response effects of in vitro exposure to PFASs and PCB congeners on mitogen-induced lymphocyte proliferation. Upon in vitro contaminant exposure, the non-coplanar PCB congeners CB 138, 153, and 180, but not the coplanar CB 169, significantly reduced lymphocyte proliferation between 10 and 20µgg-1ww. The respective in vitro EC50values for these congeners were 13.3, 20.7, 20.8, and 54.6µgg-1ww. No modulation of lymphocyte proliferation was observed upon in vitro exposure to two individual PFASs, perfluorooctane sulphonic acid (PFOS) and perfluorooctanoic acid (PFOA), at concentrations up to 1000ngg-1. In addition, no significant correlations were found between lymphocyte proliferation and any blood or blubber contaminant measured. Taken together, these data suggest this population of ringed seals is not currently at high risk of altered lymphocyte proliferation from exposure to the POPs or PFASs in this study.
authors: Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road, Storrs, CT 06269-3089, United States. Electronic address: Milton.levin@uconn.edu.
authors: Milton
authors: M
authors: Levin
authors: Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road, Storrs, CT 06269-3089, United States.
authors: Erika
authors: E
authors: Gebhard
authors: Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road, Storrs, CT 06269-3089, United States.
authors: Lindsay
authors: L
authors: Jasperse
authors: Department of Bioscience, Arctic Research Centre (ARC), Aarhus University, Frederiksborgvej 399, PO Box 358, DK-4000 Roskilde, Denmark.
authors: Jean-Pierre
authors: JP
authors: Desforges
authors: Department of Bioscience, Arctic Research Centre (ARC), Aarhus University, Frederiksborgvej 399, PO Box 358, DK-4000 Roskilde, Denmark.
authors: Rune
authors: R
authors: Dietz
authors: Department of Bioscience, Arctic Research Centre (ARC), Aarhus University, Frederiksborgvej 399, PO Box 358, DK-4000 Roskilde, Denmark.
authors: Christian
authors: C
authors: Sonne
authors: Department of Bioscience, Arctic Research Centre (ARC), Aarhus University, Frederiksborgvej 399, PO Box 358, DK-4000 Roskilde, Denmark.
authors: Igor
authors: I
authors: Eulaers
authors: Toxicological Centre, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, BE-2610 Wilrijk, Belgium.
authors: Adrian
authors: A
authors: Covaci
authors: Department of Environmental Science, Aarhus University, Frederiksborgvej 399, PO Box 358, DK-4000 Roskilde, Denmark.
authors: Rossana
authors: R
authors: Bossi
authors: Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road, Storrs, CT 06269-3089, United States.
authors: Sylvain
authors: S
authors: De Guise
mesh_terms: D017738:Alkanesulfonic Acids / Q000276:immunology / Q000633:toxicity*; D000818:Animals; D002210:Caprylates / Q000276:immunology / Q000633:toxicity*; D004785:Environmental Pollutants / Q000276:immunology / Q000633:toxicity*; D005260:Female; D005466:Fluorocarbons / Q000276:immunology / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D011078:Polychlorinated Biphenyls / Q000276:immunology / Q000633:toxicity*; D046048:Seals, Earless / Q000276:immunology*
chemical_list: D017738:Alkanesulfonic Acids; D002210:Caprylates; D004785:Environmental Pollutants; D005466:Fluorocarbons; C023036:perfluorooctanoic acid; C076994:perfluorooctane sulfonic acid; D011078:Polychlorinated Biphenyls
keywords: Immunotoxicology; Lymphocyte proliferation; PCB; PFAS; Ringed seal