title: The effect of Allium sativum (Garlic) extract on infectious bronchitis virus in specific pathogen free embryonic egg.
abstract: OBJECTIVE
  Garlic is a plant has been used as a flavor, and anti-microbial and anti-diarrheal agent. Infectious bronchitis virus (IBV) is a coronavirus. The available vaccines against IBV cannot cover new variants. This study evaluated the inhibitory effects of garlic extract on IBV.
  
  
  METHODS
  The constituents of garlic extract were detected by gas chromatography. This study was done in four groups of embryonic SPF eggs; first group was used for virus titration; second group received the mixture of different virus titration and constant amount of garlic extract; third group received 10(-3) titration of virus and after 8 hr received garlic extract and the last group received different dilutions of garlic extract.
  
  
  RESULTS
  Based on our results, in the second group, IBV vaccine strain (4/91) at all titration and M41 in 10(-2) and 10(-3) titration and in the third group both variants of virus the embryonic Index (EI) was significantly increased.
  
  
  CONCLUSIONS
  The garlic extract had inhibitory effects on IBV in the chickens embryo.
authors: Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors: Tabassom
authors: T
authors: Mohajer Shojai
authors: Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors: Arash
authors: A
authors: Ghalyanchi Langeroudi
authors: Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors: Vahid
authors: V
authors: Karimi
authors: Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors: Abbas
authors: A
authors: Barin
authors: Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors: Naser
authors: N
authors: Sadri
keywords: Antiviral; Embryonic Index; Garlic extract; Herbal medicine; Infectious Bronchitis Virus