title: Plasma polymerized carvone as an antibacterial and biocompatible coating.
abstract: Antibacterial coating is important to prevent the colonization of medical devices by biofilm forming bacteria that would cause infection and sepsis in patients. Current coating techniques such as immobilization of antimicrobial compounds, time-releasing antibiotic agents and silver nanoparticles, require multiple processing steps, and they have low efficacy and low stability. We proposed a single-step plasma polymerization of an essential oil known as carvone to produce a moderately hydrophobic antibacterial coating (ppCar) with an average roughness of <1nm. ppCar had a static water contact angle of 78°, even after 10days of air aging and it maintained its stability throughout 24h of LB broth immersion. ppCar showed promising results in the live-dead fluorescence assay and crystal violet assay. The biofilm assay showed an effective reduction of E. coli and S. aureus bacteria by 86% and 84% respectively. ppCar is also shown to rupture the bacteria membrane for its bactericidal effects. The cytotoxicity test indicated that the coating is not cytotoxic to the human cell line. This study would be of interest to researcher keen on producing a bacteria-resistance and biocompatible coating on different substrates in a cost-effective manner.
authors: Institute of Microengineering and Nanoelectronic, Universiti Kebangsaan Malaysia, Bangi 43600, Malaysia.
authors: Yuen Wah
authors: YW
authors: Chan
authors: Institute of Microengineering and Nanoelectronic, Universiti Kebangsaan Malaysia, Bangi 43600, Malaysia. Electronic address: kimsiow@ukm.edu.my.
authors: Kim Shyong
authors: KS
authors: Siow
authors: Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, 50300, Malaysia.
authors: Pei Yuen
authors: PY
authors: Ng
authors: School of Environmental and Natural Resources Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Malaysia.
authors: Usup
authors: U
authors: Gires
authors: Institute of Microengineering and Nanoelectronic, Universiti Kebangsaan Malaysia, Bangi 43600, Malaysia.
authors: Burhanuddin
authors: B
authors: Yeop Majlis
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D000081005:Cyclohexane Monoterpenes; D004926:Escherichia coli / Q000254:growth & development*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D039821:Monoterpenes / Q000737:chemistry / Q000494:pharmacology; D058626:Plasma Gases / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000254:growth & development*
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; D058626:Plasma Gases; C006923:carvone
keywords: Antibacterial coating; Bacterial infections; Biofilm; Cytotoxicity; Plasma polymerization; Roughness