title: Passive protection effect of anti-Vibrio anguillarum IgY-encapsulated feed on half-smooth tongue sole (Cynoglossus semilaevi) against V. anguillarum.
abstract: Vibrio anguillarum is one of the most harmful pathogens associated with hemorrhage septicemia syndrome in the half-smooth tongue sole (C. semilaevis) due to its high virulence. In this study, we attempted to treat half-smooth tongue sole with anti-V. anguillarum egg yolk powder to elicit a passive immunity directly against V. anguillarum infection. Anti-V. anguillarum IgY was β-cyclodextrin encapsulated in egg yolk powders as feed, which could avoid antibody inactivation in the gastrointestinal tract of half-smooth tongue sole. The IgY had an inhibiting effect on the infection of V. anguillarum in vitro. The survival rate of half-smooth tongue sole fed with basal diet containing 15% anti-V. anguillarum egg yolk powder was 70% after 7 days post-V. anguillarum challenge (10(7) CFU), which was significantly higher than those fed without anti-V. anguillarum egg yolk powder. As well, the bacterial burden in blood, liver, spleen and kidney was significantly lower in half-smooth tongue sole fed with specific IgY than those fed with non-specific IgY. These results suggested that pathogen-specific IgY may provide a valuable treatment for vibriosis infection and can be a promising food additive.
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; College of Ocean, Huaihai Institute of Technology, Lianyungang, 222005, China.
authors: Xiaojian
authors: X
authors: Gao
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China. Electronic address: zxj9307@163.com.
authors: Xiaojun
authors: X
authors: Zhang
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; College of Ocean, Huaihai Institute of Technology, Lianyungang, 222005, China.
authors: Jingjing
authors: J
authors: Sun
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
authors: Xuedi
authors: X
authors: Du
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
authors: Xiumei
authors: X
authors: Li
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
authors: Yue
authors: Y
authors: Zhang
authors: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; College of Marine Sciences, South China Normal University, Guangzhou, Guangdong, 510642, China. Electronic address: linli@mail.hzau.edu.cn.
authors: Li
authors: L
authors: Lin
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000907:Antibodies, Bacterial / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D004530:Egg Yolk / Q000737:chemistry; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D029941:Fish Proteins / Q000378:metabolism*; D005412:Flatfishes; D007136:Immunoglobulins / Q000378:metabolism*; D011208:Powders / Q000008:administration & dosage / Q000737:chemistry; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial; D029941:Fish Proteins; C016512:IgY; D007136:Immunoglobulins; D011208:Powders
keywords: Half-smooth tongue sole; Immunoglobulin Y (IgY); Passive immunity; Vibrio anguillarum