title: Three-dimensional chitin rings from body segments of a pet diplopod species: Characterization and protein interaction studies.
abstract: Physicochemical characterization of new chitin isolates can provide valuable insights into designing of biomimetic materials. Chitin isolates with a definite three-dimensional (3D) structure can exhibit characteristics that distinguish them from other chitin specimens that are in form of powder or flakes without a definite and uniform shape. Herein, 3D chitin rings were produced from body segments of a diplopod (Archispirostreptus gigas) inhabiting tropical regions. This organism is cultured easily and can reach 38cm in length, which makes it a suitable source for isolation of chitin. The chitin rings were characterized via TGA, FT-IR, SEM and XRD analyses. Enzymatic digestion test with chitinase demonstrated that chitin isolates had high purity (digestion rate: 97.4%). The source organism had high chitin content; 21.02±2.23% on dry weight. Interactions of the chitin rings with bovine serum albumin (BSA) protein were studied under different conditions (pH: 4.0-8.0, chitin amount: 6-14mg, contact time: 30-360min, protein concentration: 0.2-1mg/mL). The highest BSA adsorption was observed at pH5.0 at 20°C. The adsorption equilibrium data exhibited a better fit to Langmuir adsorption and the pseudo-first order kinetic models. The findings presented here can be useful for further studies aiming to develop biocompatible and nontoxic biomaterials.
authors: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Turkey. Electronic address: muratkaya3806@yahoo.com.
authors: Murat
authors: M
authors: Kaya
authors: Department of Biology and Plant Protection, Lithuanian University of Agriculture, LT-53361, Lithuania.
authors: Povilas
authors: P
authors: Mulerčikas
authors: Department of Chemistry, Faculty of Science, Selcuk University, 42075 Konya, Turkey.
authors: Idris
authors: I
authors: Sargin
authors: Department of Biology and Plant Protection, Lithuanian University of Agriculture, LT-53361, Lithuania.
authors: Sonata
authors: S
authors: Kazlauskaitė
authors: Department of Biology, Vytautas Magnus University, LT-44404 Kaunas, Lithuania.
authors: Vykintas
authors: V
authors: Baublys
authors: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Turkey.
authors: Bahar
authors: B
authors: Akyuz
authors: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Turkey.
authors: Esra
authors: E
authors: Bulut
authors: Department of Biology, Vytautas Magnus University, LT-44404 Kaunas, Lithuania.
authors: Vaida
authors: V
authors: Tubelytė
mesh_terms: D000327:Adsorption; D000818:Animals; D001181:Arthropods / Q000737:chemistry*; D002417:Cattle; D002686:Chitin / Q000737:chemistry*; D002688:Chitinases / Q000737:chemistry; D007700:Kinetics; D008956:Models, Chemical; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D002686:Chitin; D012710:Serum Albumin, Bovine; D002688:Chitinases
keywords: 3D chitin; Albumin; Nanofibres; Nanoporous