title: Disulfide stress in carbon monoxide poisoning.
abstract: OBJECTIVE
  Carbon monoxide (CO) remains the most common cause of lethal poisoning around the world. The purpose of this study was to investigate the homeostasis between thiol-disulfide couples and to evaluate oxidative status comprehensively in acute CO poisoning, using new parameters along with other well-known oxidant-antioxidant molecules.
  
  
  METHODS
  This case study consisted of 43 subjects who were diagnosed with carbon monoxide poisoning and 35 healthy individuals who were used as controls. Thiol-disulfide paired tests were examined in both groups using the method developed recently.
  
  
  RESULTS
  Patients with CO poisoning had significantly higher levels of serum disulfide than the control patients (20.7±5.03 versus 16.43±3.97, p=0.001). Native thiol and total thiol levels were lower in the CO patient group than in the control group (p<0.001, for each variable). The disulfide/native thiol ratios and disulfide/total thiol ratios were significantly higher, while native thiol/total thiol ratios were significantly lower, in patients with acute CO poisoning than in the healthy controls (p<0.001, for all ratios). The disulfide/native ratios were negatively correlated with both total antioxidant response and paraoxonase and arylesterase values and were positively correlated with total oxidant status and ceruloplasmin values (p<0.05, for all correlations).
  
  
  CONCLUSIONS
  Excessive disulfide levels and their related ratios were found in CO poisoning patients. In particular, the disulfide/native thiol ratio was identified as an indicator for overall oxidative status. Among CO poisoning patients, the thiol-disulfide balance was found to be impaired. Therefore, the disruption of thiol-disulfide homeostasis might be involved in CO toxicity.
authors: Department of Biochemistry, 25 Aralik State Hospital, Gaziantep, Turkey. Electronic address: erginmerve@hotmail.com.
authors: Merve
authors: M
authors: Ergin
authors: Department of Emergency Medicine, 25 Aralik State Hospital, Gaziantep, Turkey.
authors: Mustafa
authors: M
authors: Caliskanturk
authors: Department of Biochemistry, Yildirim Beyazit University, Faculty of Medicine, Ankara, Turkey.
authors: Almila
authors: A
authors: Senat
authors: Department of Biochemistry, 25 Aralik State Hospital, Gaziantep, Turkey.
authors: Onur
authors: O
authors: Akturk
authors: Department of Biochemistry, Yildirim Beyazit University, Faculty of Medicine, Ankara, Turkey.
authors: Ozcan
authors: O
authors: Erel
mesh_terms: D000293:Adolescent; D000328:Adult; D002249:Carbon Monoxide Poisoning / Q000097:blood*; D004220:Disulfides / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D055815:Young Adult
chemical_list: D004220:Disulfides
keywords: Carbon monoxide poisoning; Disulfide; Oxidative stress; Thiol; Thiol-disulfide homeostasis