title: The life cycle of Thelohanellus kitauei (Myxozoa: Myxosporea) infecting common carp (Cyprinus carpio) involves aurantiactinomyxon in Branchiura sowerbyi.
abstract: Thelohanellus kitauei is a freshwater myxosporean parasite causing intestinal giant cystic disease of common carp. To clarify the life cycle of T. kitauei, we investigated the oligochaete populations in China and Hungary. This study confirms two distinct aurantiactinomyxon morphotypes (Aurantiactinomyxon type 1 and Aurantiactinomyxon type 2) from Branchiura sowerbyi as developmental stages of the life cycle of T. kitauei. The morphological characteristics and DNA sequences of these two types are described here. Based on 18S rDNA sequence analysis, Aurantiactinomyxon type 1 (2048 bp) and Aurantiactinomyxon type 2 (2031 bp) share 99.2-99.4 %, 99.8-100 % similarity to the published sequences of T. kitauei, respectively. The 18S rDNA sequences of these two aurantiactinomyxon morphotypes share 99.4 % similarity, suggesting intraspecific variation within the taxon, possibly due to geographic origin. Phylogenetic analyses demonstrate the two aurantiactinomyxon types clustered with T. kitauei. Regardless, based on 18S rDNA synonymy, it is likely that Aurantiactinomyxon type 1 and 2 are conspecific with T. kitauei. This is the fourth elucidated two-host life cycle of Thelohanellus species and the first record of T. kitauei in Europe.
authors: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, China.
authors: Dandan
authors: D
authors: Zhao
authors: Institute for Veterinary Medical Research, Centre for Agricultural Research, HAS, POB 18, H-1581, Budapest, Hungary.|School of Marine and Environmental Science, University Malaysia Terengganu, Kuala Terengganu, Malaysia.
authors: Muhammad Hafiz
authors: MH
authors: Borkhanuddin
authors: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, China.|Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, China.
authors: Weimin
authors: W
authors: Wang
authors: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, China.|Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, China.
authors: Yang
authors: Y
authors: Liu
authors: Institute for Veterinary Medical Research, Centre for Agricultural Research, HAS, POB 18, H-1581, Budapest, Hungary.
authors: Gábor
authors: G
authors: Cech
authors: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China. zhaiyh@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, China. zhaiyh@mail.hzau.edu.cn.|Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, China. zhaiyh@mail.hzau.edu.cn.
authors: Yanhua
authors: Y
authors: Zhai
authors: Institute for Veterinary Medical Research, Centre for Agricultural Research, HAS, POB 18, H-1581, Budapest, Hungary.
authors: Csaba
authors: C
authors: Székely
mesh_terms: D000818:Animals; D033341:Arguloida / Q000469:parasitology*; D002347:Carps / Q000469:parasitology*; D002681:China; D004275:DNA, Ribosomal / Q000235:genetics; D005060:Europe; D005393:Fish Diseases / Q000469:parasitology*; D006814:Hungary; D007422:Intestines / Q000469:parasitology; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D055764:Myxozoa / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D009835:Oligochaeta / Q000469:parasitology*; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S
keywords: 18S rDNA; Aurantiactinomyxon; Branchiura sowerbyi; China; Hungary; Life cycle; Thelohanellus kitauei