title: Data on importance of hematopoietic cell derived Lipocalin 2 against gut inflammation.
abstract: The data herein is related to the research article entitled "Microbiota-inducible innate immune siderophore binding protein Lipocalin 2 is critical for intestinal homeostasis" (Singh et al., 2016) [1]. In the present article, we monitored dextran sodium sulfate (DSS)-induced colitis development upon Lipocalin 2 (Lcn2) neutralization, and examined the survival of Lcn2 deficient (Lcn2KO) mice and their WT littermates upon DSS challenge. To dissect the relative contribution of immune and non-immune cells-derived Lcn2 in mediating protection against gut inflammation, we generated respective bone marrow chimera and evaluated their susceptibility to IL-10 receptor neutralization-induced chronic colitis. Neutralization of Lcn2 in WT mice resulted in exacerbated DSS-induced colitis. Notably, mice lacking Lcn2 exhibited 100% mortality whereas only 20% mortality was observed in WT mice upon DSS challenge. Further, data from bone marrow chimera showed that immune cell-derived Lcn2 is the major contributor in conferring protection against colitis.
authors: Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802, United States.
authors: Piu
authors: P
authors: Saha
authors: Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802, United States.
authors: Vishal
authors: V
authors: Singh
authors: Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802, United States.
authors: Xia
authors: X
authors: Xiao
authors: Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802, United States.
authors: Beng San
authors: BS
authors: Yeoh
authors: Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802, United States; Department of Medicine, The Pennsylvania State University Medical Center, Hershey, PA 17033, United States.
authors: Matam
authors: M
authors: Vijay-Kumar
keywords: Bone marrow chimeras; Colitis; Gut microbiota; Inflammatory bowel disease; Siderocalin