title: Analysis of Axonemal Assembly During Ciliary Regeneration in Chlamydomonas.
abstract: Chlamydomonas reinhardtii is an outstanding model genetic organism for study of assembly of cilia. Here, methods are described for synchronization of ciliary regeneration in Chlamydomonas to analyze the sequence in which ciliary proteins assemble. In addition, the methods described allow analysis of the mechanisms involved in regulation of ciliary length, the proteins required for ciliary assembly, and the temporal expression of genes encoding ciliary proteins. Ultimately, these methods can contribute to discovery of conserved genes that when defective lead to abnormal ciliary assembly and human disease.
authors: Department of Cell Biology, Emory University, 465 Whitehead Biomedical Research Building, 615 Michael Street, Atlanta, GA, 30322, USA.
authors: Emily L
authors: EL
authors: Hunter
authors: Department of Cell Biology, Emory University, 465 Whitehead Biomedical Research Building, 615 Michael Street, Atlanta, GA, 30322, USA. wsale@emory.edu.
authors: Winfield S
authors: WS
authors: Sale
authors: Department of Cell Biology, Emory University, 465 Whitehead Biomedical Research Building, 615 Michael Street, Atlanta, GA, 30322, USA.
authors: Lea M
authors: LM
authors: Alford
mesh_terms: D001692:Biological Transport; D002478:Cells, Cultured; D002696:Chlamydomonas / Q000502:physiology*; D002923:Cilia / Q000502:physiology*; D006801:Humans; D012038:Regeneration
keywords: Axonemal assembly; Chlamydomonas; Ciliary regeneration; Deciliation; pH shock