title: Preparation and Biochemical Property of Penicillin G Amidase-Loaded Alginate and Alginate/Chitosan Hydrogel Beads.
abstract: BACKGROUND
  Penicillin G amidase (PGA) (EC 3.5.1.11) are enzymes that are mainly involved in the synthesis of semi-synthetic }-lactam antibiotics. Soluble PGA is costly and lacks long term operational stability. We revised most of the patents related to Penicillin G amidase (PGA) immobilization in the section "Recent Patents on Immobilized Penicillin G Amidase".
  
  
  OBJECTIVE
  The aim of this work was to study comparative biochemical property of PGA enzyme immobilized in two hydro-gel beads - Ca-alginate and alginate+chitosan hybrid and morphologically characterised by SEM.
  
  
  METHODS
  PGA immobilized in alginate+chitosan hybrid bead shows high pH and thermal stability. Km, Vmax and Effectiveness factor (1) value of free PGA were 56.19 mg/ml, 1.786 U/ml and 1, respectively. These parameters for PGA immobilized alginate beads were 64.84 mg/ml, 0.781U/ml and 0.437, respectively and for PGA immobilized alginate+chitosan hybrid beads were 87.08 mg/ml, 0.622 U/ml and 0.348, respectively.
  
  
  RESULTS
  Immobilized PGA on alginate+chitosan hybrid beads gave the highest thermal stability, reusability and storage stability than alginate immobilized PGA.
  
  
  CONCLUSIONS
  The entrapment of PGA on alginate+chitosan hybrid beads revealed several advantages and could be used in 6APA (6- aminopenicillanic acid) production.
authors: Neh
authors: N
authors: Nupur
authors: EmptyYN Y
authors: EY
authors: Ashish
authors: School of Biochemical Engineering, Indian Institute of Technology, (Banaras Hindu University), Varanasi-221005, U.P., India.
authors: Mira
authors: M
authors: Debnath
mesh_terms: D000464:Alginates / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010330:Patents as Topic; D010397:Penicillanic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D010399:Penicillin Amidase / Q000737:chemistry*; D010400:Penicillin G / Q000737:chemistry*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D010397:Penicillanic Acid; D020723:Glucuronic Acid; D048271:Chitosan; D010399:Penicillin Amidase; D010400:Penicillin G; C004408:aminopenicillanic acid