title: microRNA-214 Governs Lung Cancer Growth and Metastasis by Targeting Carboxypeptidase-D.
abstract: Lung cancer is one of the most malignant cancers with a high metastatic potential. The purpose of this study was to study the role and the underlying mechanism of miR-214 in lung cancer progression. The expression of miR-214 in normal lung and lung cancer tissue was analyzed by quantitative real-time PCR analysis. Furthermore, H1299 cells were infected with miR-214 lentivirus, and the effect of infection on cell viability and migration was analyzed. Carboxypeptidase-D (CPD), as a potential target of miR-214, was characterized in either normal lung or lung cancer tissues. The interaction of CPD expression with the tumor suppressing effect of miR-214 was characterized. We demonstrated that low miR-214 expression is a hallmark of lung cancer, especially high-grade and metastatic cancer. In vitro studies in H1299 cells confirmed that low miR-214 expression is associated with enhanced proliferation and migratory abilities. Similarly, CPD overexpression coincides with high-grade lung cancer and the CPD overexpression could reverse the inhibitory effects of miR-214. miR-214 is a tumor suppressor in lung cancer. miR-214 inhibits lung cancer progression by targeting CPD. The miR-214-CPD axis may be a therapeutic axis for lung cancer patients.
authors: 1 Department of Thoracic Surgery, The First Hospital of Shijiazhuang , Shijiazhuang, China .
authors: Xiaojian
authors: X
authors: Zhao
authors: 2 Department of Endocrinology, The First Hospital of Shijiazhuang , Shijiazhuang, China .
authors: Caiping
authors: C
authors: Lu
authors: 1 Department of Thoracic Surgery, The First Hospital of Shijiazhuang , Shijiazhuang, China .
authors: Weiwei
authors: W
authors: Chu
authors: 1 Department of Thoracic Surgery, The First Hospital of Shijiazhuang , Shijiazhuang, China .
authors: Yaxiao
authors: Y
authors: Zhang
authors: 1 Department of Thoracic Surgery, The First Hospital of Shijiazhuang , Shijiazhuang, China .
authors: Bing
authors: B
authors: Zhang
authors: 1 Department of Thoracic Surgery, The First Hospital of Shijiazhuang , Shijiazhuang, China .
authors: Qiang
authors: Q
authors: Zeng
authors: 1 Department of Thoracic Surgery, The First Hospital of Shijiazhuang , Shijiazhuang, China .
authors: Renfeng
authors: R
authors: Wang
authors: 1 Department of Thoracic Surgery, The First Hospital of Shijiazhuang , Shijiazhuang, China .
authors: Zhe
authors: Z
authors: Li
authors: 1 Department of Thoracic Surgery, The First Hospital of Shijiazhuang , Shijiazhuang, China .
authors: Baolei
authors: B
authors: Lv
authors: 1 Department of Thoracic Surgery, The First Hospital of Shijiazhuang , Shijiazhuang, China .
authors: Jiabao
authors: J
authors: Liu
mesh_terms: D002268:Carboxypeptidases / Q000235:genetics* / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*
chemical_list: C547397:MIRN214 microRNA, human; D035683:MicroRNAs; D002268:Carboxypeptidases; C078874:carboxypeptidase D
keywords: carboxypeptidase-D; lung cancer; metastasis; miR-214