title: Curcumin-Mediated Photodynamic Inactivation of Norovirus Surrogates.
abstract: Photodynamic inactivation (PDI) is extensively used to inactivate different type of pathogens through the use of photosensitizers (PS). Curcumin has been identified as an excellent natural photosensitizer with some potential applications in the food industry. The aim of this study was to assess the antiviral activity of photoactivated curcumin on norovirus surrogates, feline calicivirus (FCV), and murine norovirus (MNV). Initially, different concentrations of curcumin (13.5-1358 µM) were individually mixed with each virus at titers of ca. 6-7 log TCID50/ml and photoactivated by LED blue light with light dose of 3 J/cm2. Results showed that photoactivated curcumin at 50 µg/mL reduced FCV titers by almost 5 log after incubation at 37 °C for 30 min. Lower antiviral activity (0.73 log TCID50/mL reduction) was reported for MNV. At room temperature, curcumin at 5 µg/mL reduced FCV titers by 1.75 log TCID50/mL. These results represent a step forward in improving food safety using photoactivated curcumin as an alternative natural additive to reduce viral contamination.
authors: Departament of Biotechnology, Institute of Agrochemistry and Food Technology (IATA-CSIC), Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain.|Departament of Microbiology and Ecology, University of Valencia, Av. Dr. Moliner, 50, 46100, Burjassot, Valencia, Spain.
authors: W
authors: W
authors: Randazzo
authors: Departament of Biotechnology, Institute of Agrochemistry and Food Technology (IATA-CSIC), Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain.|Departament of Microbiology and Ecology, University of Valencia, Av. Dr. Moliner, 50, 46100, Burjassot, Valencia, Spain.
authors: R
authors: R
authors: Aznar
authors: Departament of Biotechnology, Institute of Agrochemistry and Food Technology (IATA-CSIC), Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain. gloriasanchez@iata.csic.es.|Departament of Microbiology and Ecology, University of Valencia, Av. Dr. Moliner, 50, 46100, Burjassot, Valencia, Spain. gloriasanchez@iata.csic.es.
authors: G
authors: G
authors: Sánchez
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D017250:Caliciviridae Infections / Q000628:therapy / Q000821:virology*; D017927:Calicivirus, Feline / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D002415:Cats; D002460:Cell Line; D003474:Curcumin / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D029322:Norovirus / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D010778:Photochemotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D038301:Virus Inactivation / Q000187:drug effects / Q000528:radiation effects
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts; D003474:Curcumin
keywords: Curcumin; Enteric virus; Natural compounds; Norovirus; Photodynamic inactivation; Photosensitizers