title: Bicontinuous Nanoporous Frameworks: Caged Longevity for Enzymes.
abstract: The preparation of bicontinuous nanoporous covalent frameworks, which are promising for caging active enzymes, is demonstrated. The frameworks have three- dimensionally continuous, hydrophilic pores with widths varying between 5 and 30 nm. Enzymes were infiltrated into the bicontinuous pore by applying a pressured enzyme solution. The new materials and methods allowed the amount of caged proteins to be controlled precisely. The resulting enzyme-loaded framework films could be recycled many times with nearly no loss of catalytic activity. Entropic trapping of proteins by a bicontinuous pore with the right size distribution is an unprecedented strategy toward facile in vitro utilization of biocatalysts.
authors: Department School of Materials Science and Engineering and Research Institute for Solar and Sustainable Energies, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju, 61005, Korea.
authors: Jae-Sung
authors: JS
authors: Bae
authors: Department School of Materials Science and Engineering and Research Institute for Solar and Sustainable Energies, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju, 61005, Korea.
authors: Eunkyung
authors: E
authors: Jeon
authors: Department School of Materials Science and Engineering and Research Institute for Solar and Sustainable Energies, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju, 61005, Korea.
authors: Su-Young
authors: SY
authors: Moon
authors: Department School of Materials Science and Engineering and Research Institute for Solar and Sustainable Energies, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju, 61005, Korea.
authors: Wangsuk
authors: W
authors: Oh
authors: Department School of Materials Science and Engineering and Research Institute for Solar and Sustainable Energies, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju, 61005, Korea.
authors: Sun-Young
authors: SY
authors: Han
authors: Department of Polymer Science and Engineering, Chungnam National University, 299 Daehak-Ro, Yuseong-Gu, Daejeon, 34134, Korea.
authors: Jeong Hun
authors: JH
authors: Lee
authors: Department of Polymer Science and Engineering, Chungnam National University, 299 Daehak-Ro, Yuseong-Gu, Daejeon, 34134, Korea. sungyun@cnu.ac.kr.
authors: Sung Yun
authors: SY
authors: Yang
authors: Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, 299 Daehak-Ro, Yuseong-Gu, Daejeon, 34134, Korea.
authors: Dong-Myung
authors: DM
authors: Kim
authors: Department School of Materials Science and Engineering and Research Institute for Solar and Sustainable Energies, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju, 61005, Korea. jiwoong@gist.ac.kr.
authors: Ji-Woong
authors: 0000-0002-8783-3675
authors: JW
authors: Park
mesh_terms: D055162:Biocatalysis; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D058608:Nanopores; D019301:Oleic Acid / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D013997:Time Factors
chemical_list: D004798:Enzymes; D004800:Enzymes, Immobilized; D019301:Oleic Acid; D011092:Polyethylene Glycols; D008049:Lipase
keywords: bicontinuous nanoporous structures; enzyme nanoreactors; microfluidics; nanoporous films; organic sol-gel synthesis