title: Effects of dispersed oil exposure on biomarker responses and growth in juvenile wolfish Anarhichas denticulatus.
abstract: This study evaluated the sensitivity of the wolfish Anarhichas denticulatus exposed to crude oil, comparing the effects of mechanically dispersed versus chemically dispersed oil using sub-lethal endpoints. To test the toxicity of this controversial technique, two experiments involving exposure of the organisms for 48 h were conducted. The first experiment assessed the effects of oil exposure on biomarker responses. The second experiment monitored the growth of juveniles over 5 weeks after exposure. Overall, this study demonstrated that polycyclic aromatic hydrocarbon (PAH) biliary metabolites, ethoxyresorufin-O-deethylase (EROD), and acetylcholinesterase (AChE) are appropriate biomarkers to assess exposure of A. denticulatus. Growth rate, both in length and weight, was significantly higher in control compared to oil exposure treatments. The lack of differences between chemically and mechanically dispersed oils in biomarker response and growth suggests that dispersant application is no more toxic than the natural oil dispersion. The results indicate the potential for population-level effects resulting from exposure to oil.
authors: Centro de Estudos do Mar, Universidade Federal do Paraná, Beira Mar s/n, PO Box 61, Pontal do Paraná, Paraná, 83255-976, Brazil. leonardosandrini@gmail.com.
authors: L
authors: http://orcid.org/0000-0003-4086-6741
authors: L
authors: Sandrini-Neto
authors: Akvaplan-niva, Fram Centre, 9296, Tromsø, Norway.
authors: P
authors: P
authors: Geraudie
authors: Departamento de Biologia Celular, Universidade Federal do Paraná, Curitiba, Paraná, 81531-980, Brazil.
authors: M S
authors: MS
authors: Santana
authors: Akvaplan-niva, Fram Centre, 9296, Tromsø, Norway.
authors: L
authors: L
authors: Camus
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D010473:Perciformes / Q000254:growth & development* / Q000378:metabolism; D010578:Petroleum / Q000032:analysis / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity*; D012623:Seawater / Q000737:chemistry; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1; D000110:Acetylcholinesterase
keywords: Arctic; Biliary metabolites; Biomarkers; Crude oil; Dispersant; Petroleum hydrocarbons