title: Molecular characterisation of parvoviruses from domestic cats reveals emergence of newer variants in India.
abstract: Objectives The present study was undertaken to characterise the viral polypeptide 2 (VP2) gene of parvovirus from domestic cats in India. Methods The faecal samples from diarrhoeic/healthy domestic cats were collected from different geographical regions of India for screening by PCR assay followed by sequence analysis of the VP2 gene. Results Canine parvovirus (CPV)/feline panleukopenia virus (FPV) infections were found in 12 (11.3%) of 106 faecal samples tested. Two new CPV-2a (297Ala and Asn426) and three FPV strains were identified by VP2 gene analysis. Several unique and existing amino acid mutations were found, suggesting continuous evolution and emergence of newer variants. The phylogenetic analysis of the CPV sequences revealed that the two new CPV-2a strains from Mumbai (MC8) and Puducherry (P15) were clustered together in a single clade but had evolved independently and were ancestrally related to Chinese CPV-2a isolates. The FPV sequences (T-C-6 and T-C-1) from Thrissur, Kerala, formed a different clade (FPV clade) and were closely related to each other and had an ancestral relationship with an FPV isolate from the USA. Another FPV isolate from Goa (GC1) was positioned in the same clade but had evolved independently. Conclusions and relevance Detection of CPV in both diarrhoeic/healthy cats and the occurrence of FPV infection in a vaccinated cat provide new insights into parvovirus infections in cats in India.
authors: 1 Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, India.
authors: Hirak K
authors: HK
authors: Mukhopadhyay
authors: 1 Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, India.
authors: Mangadevi
authors: M
authors: Nookala
authors: 1 Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, India.
authors: Nobal Rk
authors: NR
authors: Thangamani
authors: 1 Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, India.
authors: Amsaveni
authors: A
authors: Sivaprakasam
authors: 1 Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, India.
authors: Prabhakar X
authors: PX
authors: Antony
authors: 2 Department of Veterinary Biochemistry, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, India.
authors: Jacob
authors: J
authors: Thanislass
authors: 1 Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, India.
authors: Mouttou V
authors: MV
authors: Srinivas
authors: 1 Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, India.
authors: Raghavan M
authors: RM
authors: Pillai
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics*; D002415:Cats; D005243:Feces / Q000821:virology; D005254:Feline Panleukopenia / Q000821:virology*; D028301:Feline Panleukopenia Virus / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D007194:India; D008297:Male; D009154:Mutation; D017993:Parvovirus, Canine / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D036022:Capsid Proteins; C095548:capsid protein VP2, parvovirus B19