title: Biomanufacture of nano-Pd(0) by Escherichia coli and electrochemical activity of bio-Pd(0) made at the expense of H2and formate as electron donors.
abstract: OBJECTIVE
  Palladised cells of Desulfovibrio desulfuricans and Shewanella oneidensis have been reported as fuel cell electrocatalysts but growth at scale may be unattractive/costly; we have evaluated the potential of using E. coli, using H2/formate for Pd-nanoparticle manufacture.
  
  
  RESULTS
  Using 'bio-Pd' made under H2(20 wt%) cyclic voltammograms suggested electrochemical activity of bio-NPs in a native state, attributed to proton adsorption/desorption. Bio-Pd prepared using formate as the electron donor gave smaller, well separated NPs; this material showed no electrochemical properties, and hence little potential for fuel cell use using a simple preparation technique. Bio-Pd on S. oneidensis gave similar results to those obtained using E. coli.
  
  
  CONCLUSIONS
  Bio-Pd is sufficiently conductive to make an E. coli-derived electrochemically active material on intact, unprocessed bacterial cells if prepared at the expense of H2, showing potential for fuel cell applications using a simple one-step preparation method.
authors: School of Chemistry, University of East Anglia, Norwich Research Park, Norwich, Norfolk, NR4 7TJ, UK.
authors: J
authors: J
authors: Courtney
authors: Finovatis, 68 Cours Lafayette, 69003, Lyon, France.
authors: K
authors: K
authors: Deplanche
authors: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors: N V
authors: NV
authors: Rees
authors: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. L.E.Macaskie@bham.ac.uk.
authors: L E
authors: LE
authors: Macaskie
mesh_terms: D001674:Bioelectric Energy Sources; D045285:Desulfovibrio desulfuricans / Q000378:metabolism; D004563:Electrochemistry; D004583:Electrons; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism; D005561:Formates / Q000737:chemistry* / Q000378:metabolism; D006859:Hydrogen / Q000737:chemistry* / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry; D010165:Palladium / Q000737:chemistry* / Q000378:metabolism; D020592:Shewanella / Q000378:metabolism
chemical_list: D005561:Formates; C030544:formic acid; D010165:Palladium; D006859:Hydrogen
keywords: Bio-Pd; E. coli; Electrochemical activity; Fuel cell; Hydrogen production; Palladium