title: Targeting cancer cells via tumor-homing peptide CREKA functional PEG nanoparticles.
abstract: Targeting cell microenvironment via nano-particle based therapies holds great promise for the treatment of various diseases. One of the main challenges in targeted delivery of nanoparticles for cancer therapy is the reduced localization of delivery vehicles to the tumor site. The therapeutic efficacy of drugs can be improved by recruiting delivery vehicles towards specific region of tumorigenesis in the body. Here, we demonstrate an effective approach in creating PEG particles via water-in-water emulsion technique with a tumor-homing peptide CREKA functionalization. The CREKA conjugated hydrogel nanoparticles were found to be more effective at inducing Doxorubicin (DOX)-mediated apoptosis compared to that of particles conjugated with laminin peptide IKVAV. Fluorescence intensity analysis on confocal micrographs suggested significantly higher cellular uptake of CREKA conjugated PEG particles than internalization of nanoparticles in other groups. We observed that fibrin binding ability of PEG particles could be increased up to 94% through CREKA conjugation. Our results suggest the possibility of cancer cell targeting via CREKA-functional PEG nanoparticles.
authors: Biomedical Sciences and Engineering, Koç University, Istanbul 34450, Turkey.
authors: Aysu Ceren
authors: AC
authors: Okur
authors: Biomedical Sciences and Engineering, Koç University, Istanbul 34450, Turkey.
authors: Pelin
authors: P
authors: Erkoc
authors: Biomedical Sciences and Engineering, Koç University, Istanbul 34450, Turkey; Chemical and Biological Engineering, Koç University, Istanbul 34450, Turkey. Electronic address: skizilel@ku.edu.tr.
authors: Seda
authors: S
authors: Kizilel
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects*; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D016503:Drug Delivery Systems; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D000903:Antibiotics, Antineoplastic; D009842:Oligopeptides; C542438:cysteinyl-arginyl-glutamyl-lysyl-alanyl; D011092:Polyethylene Glycols; D004317:Doxorubicin
keywords: CREKA; Hydrogel nanoparticles; IKVAV; Water-in-water emulsion