title: Composite Membranes of Recombinant Silkworm Antimicrobial Peptide and Poly (L-lactic Acid) (PLLA) for biomedical application.
abstract: Antimicrobial peptides, produced by innate immune system of hosts in response to invading pathogens, are capable of fighting against a spectrum of bacteria, viruses, fungi, parasites and cancer cells. Here, a recombinant silkworm AMP Bmattacin2 from heterologous expression is studied, indicating a broad spectrum of antibacterial activity and showing selective killing ability towards skin and colon cancer cells over their normal cell counterparts. For the purpose of biomedical application, the electrospinning fabrication technique is employed to load Bmattacin2 into PLLA nanofibrous membrane. In addition to a good compatibility with the normal cells, Bmattacin2 loaded nanofibrous membranes demonstrate instant antibacterial effects and sustained anticancer effects. The cancer cell and bacteria targeting dynamics of recombinant Bmattacin2 are investigated. With these characteristics, PLLA/Bmattacin2 composite membranes have a great potential for developing novel biomedical applications such as cancer therapies and wound healing treatments.
authors: Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, College of Textiles and Garments, Southwest University, Chongqing, China.|Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors: Zhi
authors: Z
authors: Li
authors: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors: Xuan
authors: X
authors: Liu
authors: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.|School of Materials, The University of Manchester Manchester M13 9PL, UK.
authors: Yi
authors: Y
authors: Li
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors: Xiqian
authors: X
authors: Lan
authors: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors: Polly Hangmei
authors: PH
authors: Leung
authors: School of Materials, The University of Manchester Manchester M13 9PL, UK.
authors: Jiashen
authors: J
authors: Li
authors: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, China.
authors: Gang
authors: G
authors: Li
authors: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors: Maobin
authors: M
authors: Xie
authors: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors: Yanxia
authors: Y
authors: Han
authors: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors: Xiaofen
authors: X
authors: Lin
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D012831:Bombyx / Q000235:genetics; D045744:Cell Line, Tumor; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D019476:Insect Proteins; D011091:Polyesters; D011994:Recombinant Proteins; C039711:attacin antibacterial protein, insect; C033616:poly(lactide)