title: Antimicrobial susceptibility of lactic acid bacteria isolated from human and food-producing animal feces in Khon Kaen Province, Thailand.
abstract: The aim of this study was to investigate the susceptibility of 93 Lactobacillus strains to seven antimicrobial agents, i.e., penicillin G, amoxicillin-clavulanic acid, vancomycin, tetracycline, streptomycin, ciprofloxacin, and sulfamethoxazole-trimethoprim, by disk diffusion test. The Lactobacillus strains were isolated from fecal samples taken from 90 healthy, food-producing animals (fattening pigs, free-grazing ducks, and beef cattle) and 30 healthy human subjects (1- to 6-year-olds) in Khon Kaen. The minimum inhibitory concentration (MIC) values of tetracycline and ciprofloxacin against all strains were determined using the E-test. All 93 Lactobacillus isolates were identified at the species level using 16S rRNA gene sequencing. The most common species of Lactobacillus isolated from fattening pigs, free-grazing ducks, beef cattle, and humans were L. reuteri (30 %), L. salivarius (46.7 %), L. acetotolerans (20 %), and L. gasseri (33.3 %), respectively. A total of 83 Lactobacillus strains were resistant to the examined antibiotics. Some strains were resistant to two to six types of antibiotics. More than 50 % of Lactobacillus species were intrinsically resistant to vancomycin, streptomycin, ciprofloxacin, and sulfamethoxazole-trimethoprim. The prevalence of acquired resistance to tetracycline was observed for Lactobacillus isolates from fattening pigs, humans, free-grazing ducks, and beef cattle at 92.3, 85.7, 77.8, and 68.4 %, respectively. These results demonstrate the impact of antibiotic use in human and veterinary medicine on antibiotic treatment efficacy and may support the spread of transferable antibiotic resistant genes to other bacteria via the food chain.
authors: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. pirson@kku.ac.th.
authors: Pairat
authors: http://orcid.org/0000-0001-8053-7091
authors: P
authors: Sornplang
authors: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors: Kattinet
authors: K
authors: Sakulsawasdiphan
authors: Faculty of Agricultural and Technology, Rajamangala University of Technology Isan, Surin Campus, Surin, 32000, Thailand.
authors: Sudthidol
authors: S
authors: Piyadeatsoontorn
authors: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors: Benyapha
authors: B
authors: Surasorn
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy* / Q000382:microbiology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004372:Ducks; D005243:Feces / Q000382:microbiology; D006801:Humans; D007778:Lactobacillus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D000069466:Red Meat; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy* / Q000382:microbiology; D013785:Thailand; D014329:Tropical Climate
chemical_list: D000890:Anti-Infective Agents; D012336:RNA, Ribosomal, 16S
keywords: Antibiotic resistance; Food-producing animals; Humans; Lactobacillus species