title: Structural characterization and antiviral effect of a novel polysaccharide PSP-2B from Prunellae Spica.
abstract: In the present study, a novel polysaccharide, PSP-2B, was isolated from aqueous extracts of Prunellae Spica by direct ultrafiltration membrane separation and gel chromatography purification. PSP-2B is a partially sulphated polysaccharide with a molecular weight of approximately 32kDa. Its sulfate content is 10.59% by elemental analysis. The major sugars comprising PSP-2B are arabinose, galactose and mannose, in addition to small amounts of glucose and uronic acids. The framework of PSP-2B is speculated to be a branched arabinogalactomannan, and the side chains are terminated primarily by the Araf residues. PSP-2B also contains 2.98% protein. PSP-2B exhibits activity against herpes simplex virus (HSV), with a half maximal inhibitory concentration (IC50) of approximately 69μg/mL for HSV-1 and 49μg/mL for HSV-2. However, PSP-2B demonstrated no cytotoxicity even when its concentration was increased to 1600μg/mL, suggesting that it has potential as an anti-HSV drug candidate.
authors: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
authors: Feng-Wei
authors: FW
authors: Ma
authors: Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China.
authors: Si-Yuan
authors: SY
authors: Kong
authors: Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China.
authors: Hong-Sheng
authors: HS
authors: Tan
authors: Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China.
authors: Rong
authors: R
authors: Wu
authors: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
authors: Bing
authors: B
authors: Xia
authors: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. Electronic address: zhouyan@cib.ac.cn.
authors: Yan
authors: Y
authors: Zhou
authors: Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China. Electronic address: xuhongxi88@gmail.com.
authors: Hong-Xi
authors: HX
authors: Xu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002236:Carbohydrate Conformation; D002522:Chlorocebus aethiops; D006561:Herpes Simplex / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D018259:Herpesvirus 1, Human / Q000378:metabolism*; D006801:Humans; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D031347:Prunella / Q000737:chemistry*; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D011134:Polysaccharides
keywords: Against herpes simplex virus activity; Arabinose (PubChem CID: 5460291); Galactose (PubChem CID: 439357); Galacturonic acid (PubChem CID: 84740); Glucose (PubChem CID: 5793); Glucuronic acid (PubChem CID: 441478); Mannose (PubChem CID: 18950); Prunellae Spica; Structural characterization; Sulphated polysaccharide