title: Bacterial Adhesion and Surface Roughness for Different Clinical Techniques for Acrylic Polymethyl Methacrylate.
abstract: This study sought to assess the effect of different surface finishing and polishing protocols on the surface roughness and bacterial adhesion (S. sanguinis) to polymethyl methacrylates (PMMA). Fifty specimens were divided into 5 groups (n = 10) according to their fabrication method and surface finishing protocol: LP (3 : 1 ratio and laboratory polishing), NF (Nealon technique and finishing), NP (Nealon technique and manual polishing), MF (3 : 1 ratio and manual finishing), and MP (3 : 1 ratio and manual polishing). For each group, five specimens were submitted to bacterial adhesion tests and analyzed by scanning electron microscopy (SEM). Two additional specimens were subjected to surface topography analysis by SEM and the remaining three specimens were subjected to surface roughness measurements. Data were compared by one-way ANOVA. The mean bacterial counts were as follows: NF, 19.6 ± 3.05; MP, 5.36 ± 2.08; NP, 4.96 ± 1.93; MF, 7.36 ± 2.45; and LP, 1.56 ± 0.62 (CFU). The mean surface roughness values were as follows: NF, 3.23 ± 0.15; MP, 0.52 ± 0.05; NP, 0.60 ± 0.08; MF, 2.69 ± 0.12; and LP, 0.07 ± 0.02 (μm). A reduction in the surface roughness was observed to be directly related to a decrease in bacterial adhesion. It was verified that the laboratory processing of PMMA might decrease the surface roughness and consequently the adhesion of S. sanguinis to this material.
authors: Department of Fixed Prostheses, School of Dentistry, State University of Rio Grande do Norte, Caicó, RN, Brazil.
authors: Lucas Costa de Medeiros
authors: 0000-0002-0920-5366
authors: LC
authors: Dantas
authors: Department of Dentistry, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho, s/n, Natal, RN, Brazil.
authors: João Paulo
authors: 0000-0002-3140-2403
authors: JP
authors: da Silva-Neto
authors: LZNA Institute, Rua Alexandre Marquez 477, Uberlândia, MG, Brazil.
authors: Talita Souza
authors: 0000-0003-3785-0343
authors: TS
authors: Dantas
authors: LZNA Institute, Rua Alexandre Marquez 477, Uberlândia, MG, Brazil.
authors: Lucas Zago
authors: 0000-0003-2286-7636
authors: LZ
authors: Naves
authors: Department of Fixed Prostheses, Occlusion and Dental Materials, School of Dentistry, Federal University of Uberlândia, Campus Umuarama, Avenida Pará No. 1720, Uberlândia, MG, Brazil.
authors: Flávio Domingues
authors: FD
authors: das Neves
authors: Department of Fixed Prostheses, Occlusion and Dental Materials, School of Dentistry, Federal University of Uberlândia, Campus Umuarama, Avenida Pará No. 1720, Uberlândia, MG, Brazil.
authors: Adérito Soares
authors: AS
authors: da Mota