title: Effect of Mg content on the bioactivity and biocompatibility of Mg-substituted fluorapatite nanopowders fabricated via mechanical activation.
abstract: The aim of this work was preparation, characterization, bioactivity and biocompatibility evaluation of Mg-substituted fluorapatite (Mg-FA) nanopowders. Mg-FA nanopowders with a chemical composition of Ca10-xMgx(PO4)6F2, with x=0, 0.5, 1, and 2 were prepared by mechanically activated method. The in vitro bioactivity was investigated by soaking the powders in simulated body fluid (SBF) for various time periods to analyze the nucleation and growth of bone-like apatite on the surface of the samples. Cell viability and cell attachment were studied by MTT assay. Results indicated that the bioactivity of all of samples with different Mg content was improved compared with the pure FA. However, the mechanism of bioactivity is different and depends on the amount of Mg substitution. Finally, cell culture suggested that the addition of Mg(2+) has no adverse effect and Mg-FA samples have good biocompatibility. The Mg-FA material shows potential in satisfying the requirements of biomedical applications.
authors: Biomaterials Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 8415683111, Iran; School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, Tehran, Iran. Electronic address: mkh_fard@yahoo.com.
authors: M
authors: M
authors: Kheradmandfard
authors: Biomaterials Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 8415683111, Iran; Dental Materials Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: M H
authors: MH
authors: Fathi
authors: Biomaterials Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 8415683111, Iran.
authors: F
authors: F
authors: Ansari
authors: Department of Materials Engineering, Shahreza Branch, Islamic Azad university, 86137 Isfahan, Iran.
authors: T
authors: T
authors: Ahmadi
mesh_terms: D001031:Apatites / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D001826:Body Fluids / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008274:Magnesium / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*; D011208:Powders
chemical_list: D001031:Apatites; D011208:Powders; D008274:Magnesium; C025105:fluorapatite
keywords: Bioactivity; Biocompatibility; Magnesium-substituted fluorapatite; Mechanical alloying; Nanostructured materials; Simulated body fluid