title: Ultra-thin layer chromatography with integrated silver colloid-based SERS detection.
abstract: Simplified lab-on-a-chip techniques are desirable for quick and efficient detection of analytes of interest in the field. The following work involves the use of deterministic pillar arrays on the micro-scale as a platform to separate compounds, and the use of Ag colloid within the arrays as a source of increased signal via surface enhanced Raman spectroscopy (SERS). One problem traditionally seen with SERS surfaces containing Ag colloid is oxidation; however, our platforms are superhydrophobic, reducing the amount of oxidation taking place on the surface of the Ag colloid. This work includes the successful separation and SERS detection of a fluorescent dye compounds (resorufin and sulforhodamine 640), fluorescent anti-tumor drugs (Adriamycin and Daunomycin), and purine and pyrimidine bases (adenine, cytosine, guanine, hypoxanthine, and thymine).
authors: Department of Chemistry, University of Tennessee, Knoxville, TN, USA.
authors: Ryan A
authors: RA
authors: Wallace
authors: Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
authors: Nickolay V
authors: NV
authors: Lavrik
authors: Department of Chemistry, University of Tennessee, Knoxville, TN, USA.
authors: Michael J
authors: MJ
authors: Sepaniak
mesh_terms: D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; C586932:colloidal silver; D012834:Silver
keywords: Photolithography; SERS; Ultra-thin layer chromatograpy