title: Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle.
abstract: Skeletal muscle contains two distinct stem/progenitor populations. One is the satellite cell, which acts as a muscle stem cell, and the other is the mesenchymal progenitor, which contributes to muscle pathogeneses such as fat infiltration and fibrosis. Detailed and accurate characterization of these progenitors in humans remains elusive. Here, we performed comprehensive cell-surface protein profiling of the two progenitor populations residing in human skeletal muscle and identified three previously unrecognized markers: CD82 and CD318 for satellite cells and CD201 for mesenchymal progenitors. These markers distinguish myogenic and mesenchymal progenitors, and enable efficient isolation of the two types of progenitors. Functional study revealed that CD82 ensures expansion and preservation of myogenic progenitors by suppressing excessive differentiation, and CD201 signaling favors adipogenesis of mesenchymal progenitors. Thus, cell-surface proteins identified here are not only useful markers but also functionally important molecules, and provide valuable insight into human muscle biology and diseases.
authors: Division for Therapies Against Intractable Diseases, Institute for Comprehensive Medical Science, Fujita Health University, 1-98 Dengakugakubo, Kutsukake, Toyoake, Aichi 470-1192, Japan. Electronic address: uezumi@fujita-hu.ac.jp.
authors: Akiyoshi
authors: A
authors: Uezumi
authors: Division for Therapies Against Intractable Diseases, Institute for Comprehensive Medical Science, Fujita Health University, 1-98 Dengakugakubo, Kutsukake, Toyoake, Aichi 470-1192, Japan.
authors: Masashi
authors: M
authors: Nakatani
authors: Department of Regenerative Medicine, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, 35 Gengo, Morioka, Obu, Aichi 474-8511, Japan.
authors: Madoka
authors: M
authors: Ikemoto-Uezumi
authors: Laboratory of Molecular Biology & Histochemistry, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
authors: Naoki
authors: N
authors: Yamamoto
authors: Department of Orthopaedic Surgery, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
authors: Mitsuhiro
authors: M
authors: Morita
authors: Department of Orthopaedic Surgery, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
authors: Asami
authors: A
authors: Yamaguchi
authors: Department of Orthopaedic Surgery, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
authors: Harumoto
authors: H
authors: Yamada
authors: Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya, Aichi 466-8550, Japan.
authors: Takehiro
authors: T
authors: Kasai
authors: Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawa-higashi, Kodaira, Tokyo 187-8502, Japan.
authors: Satoru
authors: S
authors: Masuda
authors: Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawa-higashi, Kodaira, Tokyo 187-8502, Japan.
authors: Asako
authors: A
authors: Narita
authors: Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawa-higashi, Kodaira, Tokyo 187-8502, Japan.
authors: Yuko
authors: Y
authors: Miyagoe-Suzuki
authors: Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawa-higashi, Kodaira, Tokyo 187-8502, Japan.
authors: Shin'ichi
authors: S
authors: Takeda
authors: Department of Immunology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors: So-Ichiro
authors: S
authors: Fukada
authors: Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawa-higashi, Kodaira, Tokyo 187-8502, Japan.
authors: Ichizo
authors: I
authors: Nishino
authors: Division for Therapies Against Intractable Diseases, Institute for Comprehensive Medical Science, Fujita Health University, 1-98 Dengakugakubo, Kutsukake, Toyoake, Aichi 470-1192, Japan.
authors: Kunihiro
authors: K
authors: Tsuchida
mesh_terms: D050156:Adipogenesis; D000906:Antibodies / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers; D002462:Cell Membrane / Q000378:metabolism*; D002469:Cell Separation; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology*; D040901:Proteomics / Q000379:methods*; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D015415:Biomarkers; D008565:Membrane Proteins