title: Advanced Glycation End-Products Enhance Lung Cancer Cell Invasion and Migration.
abstract: Effects of carboxymethyllysine (CML) and pentosidine, two advanced glycation end-products (AGEs), upon invasion and migration in A549 and Calu-6 cells, two non-small cell lung cancer (NSCLC) cell lines were examined. CML or pentosidine at 1, 2, 4, 8 or 16 μmol/L were added into cells. Proliferation, invasion and migration were measured. CML or pentosidine at 4-16 μmol/L promoted invasion and migration in both cell lines, and increased the production of reactive oxygen species, tumor necrosis factor-α, interleukin-6 and transforming growth factor-β1. CML or pentosidine at 2-16 μmol/L up-regulated the protein expression of AGE receptor, p47(phox), intercellular adhesion molecule-1 and fibronectin in test NSCLC cells. Matrix metalloproteinase-2 protein expression in A549 and Calu-6 cells was increased by CML or pentosidine at 4-16 μmol/L. These two AGEs at 2-16 μmol/L enhanced nuclear factor κ-B (NF-κ B) p65 protein expression and p38 phosphorylation in A549 cells. However, CML or pentosidine at 4-16 μmol/L up-regulated NF-κB p65 and p-p38 protein expression in Calu-6 cells. These findings suggest that CML and pentosidine, by promoting the invasion, migration and production of associated factors, benefit NSCLC metastasis.
authors: Department of Respiratory Therapy, China Medical University, 40402 Taichung City, Taiwan. derrick.hsia@msa.hinet.net.|Department of Internal Medicine, China Medical University Hospital, 40402 Taichung City, Taiwan. derrick.hsia@msa.hinet.net.
authors: Te-Chun
authors: TC
authors: Hsia
authors: Department of Nutrition, China Medical University, 40402 Taichung City, Taiwan. mcyin@mail.cmu.edu.tw.|Department of Health and Nutrition Biotechnology, Asia University, 41354 Taichung City, Taiwan. mcyin@mail.cmu.edu.tw.
authors: Mei-Chin
authors: MC
authors: Yin
authors: Department of Health and Nutrition Biotechnology, Asia University, 41354 Taichung City, Taiwan. mmong@asia.edu.tw.
authors: Mei-Chin
authors: MC
authors: Mong
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D005353:Fibronectins / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism*; D008239:Lysine / Q000031:analogs & derivatives / Q000494:pharmacology; D020778:Matrix Metalloproteinase 2; D017382:Reactive Oxygen Species / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D005353:Fibronectins; D017127:Glycation End Products, Advanced; D015850:Interleukin-6; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; C048496:N(6)-carboxymethyllysine; D001120:Arginine; C062187:pentosidine; D020778:Matrix Metalloproteinase 2; D008239:Lysine
keywords: CML; invasion; migration; non-small cell lung cancer; pentosidine