title: Direct PCR-Ribotyping of Clostridium difficile.
abstract: PCR-ribotyping, a method based on heterogeneity of ribosomal intergenic spacer region, is the preferred method for genotyping of Clostridium difficile. Standardly used procedure for PCR-ribotyping is culturing of C. difficile from fecal samples and subsequent typing. In this chapter, we describe a modified PCR-ribotyping method for direct detection of PCR-ribotypes directly in total stool DNA extract, without prior need to isolate C. difficile.
authors: National Laboratory for Health, Environment and Food (NLZOH), Prvomajska 1, 2000, Maribor, Slovenia. sandra.janezic@nlzoh.si.|Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000, Maribor, Slovenia. sandra.janezic@nlzoh.si.
authors: Sandra
authors: S
authors: Janezic
mesh_terms: D016360:Clostridioides difficile / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D021901:DNA, Intergenic / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D009876:Operon; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D021521:Ribotyping / Q000379:methods*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D021901:DNA, Intergenic; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S
keywords: Clostridium difficile; Direct typing; PCR-ribotyping; Total stool DNA