title: Minibrain drives the Dacapo-dependent cell cycle exit of neurons in the Drosophila brain by promoting asense and prospero expression.
abstract: A key aim of neurodevelopmental research is to understand how precursor cells decide to stop dividing and commence their terminal differentiation at the correct time and place. Here, we show that minibrain (mnb), the Drosophila ortholog of the Down syndrome candidate gene DYRK1A, is transiently expressed in newborn neuronal precursors known as ganglion cells (GCs). Mnb promotes the cell cycle exit of GCs through a dual mechanism that regulates the expression of the cyclin-dependent kinase inhibitor Dacapo, the homolog of vertebrate p27(Kip1) (Cdkn1b). Mnb upregulates the expression of the proneural transcription factor (TF) Asense, which promotes Dacapo expression. Mnb also induces the expression of Prospero, a homeodomain TF that in turn inhibits the expression of Deadpan, a pan-neural TF that represses dacapo In addition to its effects on Asense and Prospero, Mnb also promotes the expression of the neuronal-specific RNA regulator Elav, strongly suggesting that Mnb facilitates neuronal differentiation. These actions of Mnb ensure the precise timing of neuronal birth, coupling the mechanisms that regulate neurogenesis, cell cycle control and terminal differentiation of neurons.
authors: Instituto de Neurociencias, CSIC and Universidad Miguel Hernandez, Alicante 03550, Spain.
authors: Mirja N
authors: MN
authors: Shaikh
authors: Instituto de Neurociencias, CSIC and Universidad Miguel Hernandez, Alicante 03550, Spain.
authors: Francisco
authors: F
authors: Gutierrez-Aviño
authors: Instituto de Neurociencias, CSIC and Universidad Miguel Hernandez, Alicante 03550, Spain.
authors: Jordi
authors: J
authors: Colonques
authors: Instituto de Neurociencias, CSIC and Universidad Miguel Hernandez, Alicante 03550, Spain.
authors: Julian
authors: J
authors: Ceron
authors: Instituto de Neurociencias, CSIC and Universidad Miguel Hernandez, Alicante 03550, Spain.
authors: Barbara
authors: B
authors: Hämmerle
authors: Instituto de Neurociencias, CSIC and Universidad Miguel Hernandez, Alicante 03550, Spain f.tejedor@umh.es.
authors: Francisco J
authors: 0000-0003-1942-2580
authors: FJ
authors: Tejedor
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism; D004330:Drosophila / Q000166:cytology* / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000097603:Dyrk Kinases
chemical_list: D050760:Cyclin-Dependent Kinase Inhibitor p27; D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D014157:Transcription Factors; C407353:ase protein, Drosophila; C073099:pros protein, Drosophila; C092066:mnb protein, Drosophila; D000097603:Dyrk Kinases
keywords: DYRK1A; Neural progenitor; Neural proliferation; Neurogenesis; Neuronal differentiation; Neuronal precursor