title: Interactions between mitochondrial and nuclear DNA in mammalian cells are non-random.
abstract: Chromosome Conformation Capture techniques regularly detect physical interactions between mitochondrial and nuclear DNA (i.e. mito-nDNA interactions) in mammalian cells. We have evaluated mito-nDNA interactions captured by HiC and Circular Chromosome Conformation Capture (4C). We show that these mito-nDNA interactions are statistically significant and shared between biological and technical replicates. The most frequent interactions occur with repetitive DNA sequences, including centromeres in human cell lines and the 18S rDNA in mouse cortical astrocytes. Our results demonstrate a degree of selective regulation in the identity of the interacting mitochondrial partners confirming that mito-nDNA interactions in mammalian cells are not random.
authors: Liggins Institute, University of Auckland, New Zealand.
authors: M D
authors: MD
authors: Doynova
authors: Department of Anatomy, Brain and Health Research Centre, University of Otago, Dunedin, New Zealand.
authors: A
authors: A
authors: Berretta
authors: Centre for Mathematical Biology, Massey University, Albany, New Zealand.
authors: M B
authors: MB
authors: Jones
authors: Department of Anatomy, Brain and Health Research Centre, University of Otago, Dunedin, New Zealand; Gravida: National Centre for Growth and Development, University of Auckland, Auckland, New Zealand.
authors: C L
authors: CL
authors: Jasoni
authors: Liggins Institute, University of Auckland, New Zealand; Gravida: National Centre for Growth and Development, University of Auckland, Auckland, New Zealand.
authors: M H
authors: MH
authors: Vickers
authors: Liggins Institute, University of Auckland, New Zealand; Gravida: National Centre for Growth and Development, University of Auckland, Auckland, New Zealand. Electronic address: justin.osullivan@auckland.ac.nz.
authors: J M
authors: JM
authors: O'Sullivan
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000235:genetics*
chemical_list: D004247:DNA
keywords: 4C; Centromeres; Communication; HiC; Mammalian cells; Mitochondria; Mitochondrial-nuclear DNA-DNA interactions; rDNA