title: Multidrug resistance mediated by co-carriage of extended-spectrum beta-lactamases, AmpC and New Delhi metallo-beta-lactamase-1 genes among carbapenem-resistant Enterobacteriaceae at five Indian medical centres.
abstract: In this study, we evaluated the coexistence of extended-spectrum beta-lactamases (ESBL), AmpC and New Delhi metallo-beta-lactamase-1 (NDM-1) genes among carbapenem-resistant Enterobacteriaceae (CRE) recovered prospectively from patients at multiple sites. The study included 285 CRE strains from 2782 Gram-negative Bacilli collected from multiple centres during 2007-2010, of which 87 were characterised. Standard and reference laboratory methods were used for resistance determination. Detection of blaNDM-1 , blaAmpC , blaTEM , blaSHV and blaCTX-M was done by polymerase chain reaction. High levels of antimicrobial resistance observed among study isolates. Co-carriage of ESBLs, AmpC and NDM-1 was 26.3%. Nosocomial origin among the co-carriage isolates was 64.3%, with 9.2% associated mortality.
authors: Pushpagiri Research Center, Pushpagiri Institute of Medical Sciences and Research Center, Thiruvalla, Kerala, India.
authors: A
authors: A
authors: Manoharan
authors: Department of Medicine, Benjamin M Pulimood Laboratories for Infection, Immunity and Inflammation, Christian Medical College, Vellore, Tamil Nadu, India.
authors: G S
authors: GS
authors: Barla
authors: Department of Medicine, Benjamin M Pulimood Laboratories for Infection, Immunity and Inflammation, Christian Medical College, Vellore, Tamil Nadu, India.
authors: R
authors: R
authors: Peter
authors: Department of Medicine, Benjamin M Pulimood Laboratories for Infection, Immunity and Inflammation, Christian Medical College, Vellore, Tamil Nadu, India.
authors: M
authors: M
authors: Sugumar
authors: Department of Medicine, Apollo Institute of Medical Sciences, Education and Research, Hyderabad, Telangana, India.
authors: D
authors: D
authors: Mathai
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism*; D015780:Carbapenems / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D007194:India; D008826:Microbial Sensitivity Tests; D011446:Prospective Studies; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1