title: Identification of uterine leiomyoma-specific marker genes based on DNA methylation and their clinical application.
abstract: Differential diagnosis of uterine leiomyomas and leiomyosarcomas is needed to determine whether the uterus can be retained. Therefore, biomarkers for uterine leiomyomas, and reliable and objective diagnostic methods have been desired besides the pathological diagnosis. In the present study, we identified 12 genes specific to uterine leiomyomas based on DNA methylation. Using these marker genes specific to uterine leiomyomas, we established a hierarchical clustering system based on the DNA methylation level of the marker genes, which could completely differentiate between uterine leiomyomas and normal myometrium. Furthermore, our hierarchical clustering system completely discriminated uterine cancers and differentiated between uterine leiomyosarcomas and leiomyomas with more than 70% accuracy. In conclusion, this study identified DNA methylation-based marker genes specific to uterine leiomyomas, and our hierarchical clustering system using these marker genes was useful for differential diagnosis of uterine leiomyomas and leiomyosarcomas.
authors: Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Minamikogushi 1-1-1, Ube, 755-8505 Japan.
authors: Shun
authors: S
authors: Sato
authors: Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Minamikogushi 1-1-1, Ube, 755-8505 Japan.
authors: Ryo
authors: R
authors: Maekawa
authors: Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Minamikogushi 1-1-1, Ube, 755-8505 Japan.
authors: Yoshiaki
authors: Y
authors: Yamagata
authors: Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Minamikogushi 1-1-1, Ube, 755-8505 Japan.
authors: Isao
authors: I
authors: Tamura
authors: Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Minamikogushi 1-1-1, Ube, 755-8505 Japan.
authors: Lifa
authors: L
authors: Lee
authors: Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Minamikogushi 1-1-1, Ube, 755-8505 Japan.
authors: Maki
authors: M
authors: Okada
authors: Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Minamikogushi 1-1-1, Ube, 755-8505 Japan.
authors: Kosuke
authors: K
authors: Jozaki
authors: Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Minamikogushi 1-1-1, Ube, 755-8505 Japan.
authors: Hiromi
authors: H
authors: Asada
authors: Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Minamikogushi 1-1-1, Ube, 755-8505 Japan.
authors: Hiroshi
authors: H
authors: Tamura
authors: Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Minamikogushi 1-1-1, Ube, 755-8505 Japan.
authors: Norihiro
authors: N
authors: Sugino
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D045744:Cell Line, Tumor; D004247:DNA / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D007889:Leiomyoma / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D007890:Leiomyosarcoma / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D056892:Mediator Complex / Q000235:genetics; D009215:Myometrium / Q000378:metabolism / Q000473:pathology*; D014594:Uterine Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D005819:Genetic Markers; C460107:MED12 protein, human; D056892:Mediator Complex; D004247:DNA