title: Predictive value of maternal serum NF-κB p65 and sTREM-1 for subclinical chorioamnionitis in premature rupture of membranes.
abstract: The aim of this study was to investigate the levels of nuclear factor kappa B-p65 (NF-κB p65) and soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) in maternal blood with premature rupture of membranes (PROM) and to assess their values for prediction of subclinical chorioamnionitis.
  
  
  
  NF-κB p65 and sTREM-1 levels were measured in maternal blood and cord blood by fluorescence quantitative RT-PCR assay. According to the placental membranes pathological examination, pregnant women with PROM were divided into chorioamnionitis group (n=28) and non-chorioamnionitis group (n=22).
  
  
  
  In the PROM group,the NF-κB p65 and sTREM-1 levels in maternal blood were significantly higher in women with chorioamnionitis than women without chorioamnionitis (P<.05). The cutoff value of maternal NF-κB p65, sTREM-1, C-reactive protein (CRP), and WBC level were 6.73, 2.93, 6.75 mg/L, and 10.8×10(9) /L, respectively, through analysis of the area under the ROC curve (AUC). The optimal combination test was detection of maternal blood NF-κB p65 and CRP levels, which resulted in a sensitivity of 89.3% and a specificity of 72.7% for the prediction of subclinical chorioamnionitis.
  
  
  
  Combined measurements of maternal NF-κB p65 and CRP levels may be used as early biological indicators that predict subclinical chorioamnionitis in premature rupture of membranes.
authors: Department of Obstetrics, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors: Fan
authors: F
authors: Wang
authors: Department of Obstetrics, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors: Yuhuan
authors: Y
authors: Wang
authors: Department of Obstetrics, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors: Rongyue
authors: R
authors: Wang
authors: Department of Obstetrics, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors: Haifan
authors: H
authors: Qiu
authors: Department of Obstetrics, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors: Lulu
authors: L
authors: Chen
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D002821:Chorioamnionitis / Q000097:blood* / Q000276:immunology; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000097:blood* / Q000276:immunology; D006801:Humans; D011237:Predictive Value of Tests; D011247:Pregnancy; D051996:Transcription Factor RelA / Q000097:blood* / Q000276:immunology; D000073871:Triggering Receptor Expressed on Myeloid Cells-1 / Q000097:blood* / Q000276:immunology
chemical_list: D015415:Biomarkers; C498143:RELA protein, human; C408842:TREM1 protein, human; D051996:Transcription Factor RelA; D000073871:Triggering Receptor Expressed on Myeloid Cells-1
keywords: C-reactive protein; NF-κB p65; preterm rupture of membranes; sTREM-1; subclinical chorioamnionitis