title: [Toxic effect of trichloroethylene on liver cells with CYP3A4 gene defect].
abstract: OBJECTIVE
  To investigate the toxic effect of trichloroethylene on liver cells with CYP3A4 gene defect.
  
  
  METHODS
  The normal human liver cells (L02 cells) and liver cells with CYP3A4 gene defect were exposed to trichloroethylene at different doses (0.0, 0.4, 0.8, 1.6, 3.2, and 6.4 mmol/L). CCK8 assay and RT-qPCR were used to measure cell viability and changes in the expression of apoptosis genes and oncogenes.
  
  
  RESULTS
  After being exposed to trichloroethylene at doses of 1.6, 3.2, and 6.4 mmol/L, the liver cells with CYP3A4 gene defect showed significantly higher cell viability than L02 cells (0.91±0.06/0.89±0.05/0.85±0.07 vs 0.80±0.04/0.73±0.06/0.67±0.07, P<0.05). The L02 cells in the 0.8~3.2 mmol/L trichloroethylene groups showed significant increases in the expression of the apoptosis genes caspase-3, caspase-8, and caspase-9 (P<0.05) , as well as the oncogenes c-myc, c-fos, and k-ras (P<0.05). Compared with the L02 cells, the cells with CYP3A4 gene defect showed significant reductions in the expression of the apoptosis genes caspase-3, caspase-8, and caspase-9 and the oncogenes c-myc, c-fos, and k-ras (P<0.05).
  
  
  CONCLUSIONS
  Trichloroethylene exposure has a less effect on the expression of apoptosis genes and oncogenes in liver cells with CYP3A4 gene defect than in normal human liver cells, suggesting that CYP3A4 gene defect reduces the inductive effect of trichloroethylene on apoptosis genes and oncogenes.
authors: R Y
authors: RY
authors: Liao
authors: S
authors: S
authors: Liu
mesh_terms: D017209:Apoptosis; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D002460:Cell Line; D002470:Cell Survival; D051544:Cytochrome P-450 CYP3A; D022781:Hepatocytes; D006801:Humans; D008099:Liver; D009857:Oncogenes; D016760:Proto-Oncogene Proteins c-fos; D014241:Trichloroethylene
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D014241:Trichloroethylene; D051544:Cytochrome P-450 CYP3A; C505407:CASP3 protein, human; C505530:CASP8 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9