title: Glutaredoxin GRXS17 Associates with the Cytosolic Iron-Sulfur Cluster Assembly Pathway.
abstract: Cytosolic monothiol glutaredoxins (GRXs) are required in iron-sulfur (Fe-S) cluster delivery and iron sensing in yeast and mammals. In plants, it is unclear whether they have similar functions. Arabidopsis (Arabidopsis thaliana) has a sole class II cytosolic monothiol GRX encoded by GRXS17 Here, we used tandem affinity purification to establish that Arabidopsis GRXS17 associates with most known cytosolic Fe-S assembly (CIA) components. Similar to mutant plants with defective CIA components, grxs17 loss-of-function mutants showed some degree of hypersensitivity to DNA damage and elevated expression of DNA damage marker genes. We also found that several putative Fe-S client proteins directly bind to GRXS17, such as XANTHINE DEHYDROGENASE1 (XDH1), involved in the purine salvage pathway, and CYTOSOLIC THIOURIDYLASE SUBUNIT1 and CYTOSOLIC THIOURIDYLASE SUBUNIT2, both essential for the 2-thiolation step of 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) modification of tRNAs. Correspondingly, profiling of the grxs17-1 mutant pointed to a perturbed flux through the purine degradation pathway and revealed that it phenocopied mutants in the elongator subunit ELO3, essential for the mcm5tRNA modification step, although we did not find XDH1 activity or tRNA thiolation to be markedly reduced in the grxs17-1 mutant. Taken together, our data suggest that plant cytosolic monothiol GRXs associate with the CIA complex, as in other eukaryotes, and contribute to, but are not essential for, the correct functioning of client Fe-S proteins in unchallenged conditions.
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Sabrina
authors: 0000-0001-5820-7109
authors: S
authors: Iñigo
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Astrid Nagels
authors: AN
authors: Durand
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Andrés
authors: A
authors: Ritter
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Sabine
authors: 0000-0001-8849-3812
authors: S
authors: Le Gall
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Martin
authors: 0000-0002-5618-4633
authors: M
authors: Termathe
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Roland
authors: R
authors: Klassen
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Takayuki
authors: T
authors: Tohge
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Barbara
authors: 0000-0002-9349-5086
authors: B
authors: De Coninck
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Jelle
authors: 0000-0002-4932-8192
authors: J
authors: Van Leene
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Rebecca
authors: 0000-0002-4068-1811
authors: R
authors: De Clercq
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Bruno P A
authors: 0000-0001-8087-4768
authors: BP
authors: Cammue
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Alisdair R
authors: AR
authors: Fernie
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Kris
authors: 0000-0002-4237-0283
authors: K
authors: Gevaert
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Geert
authors: 0000-0001-6558-5669
authors: G
authors: De Jaeger
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Sebastian A
authors: SA
authors: Leidel
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Raffael
authors: 0000-0001-9484-5247
authors: R
authors: Schaffrath
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Mieke
authors: 0000-0002-7632-1463
authors: M
authors: Van Lijsebettens
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.).
authors: Laurens
authors: L
authors: Pauwels
authors: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., B.D.C., J.V.L., R.D.C., B.P.A.C., G.D.J., M.V.L., L.P., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (S.I., A.N.D., A.R., S.L.G., J.V.L., R.D.C., G.D.J., M.V.L., L.P., A.G.);Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Muenster, Germany (M.T., S.A.L.);Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, D-34132 Kassel, Germany (R.K., R.S.);Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany (T.T., A.R.F.);Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium (B.D.C., B.P.A.C.);Cells-in-Motion Cluster of Excellence (M.T., S.A.L.) and Faculty of Medicine (S.A.L.), University of Muenster, 48149 Muenster, Germany;Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, B-9000 Ghent, Belgium (K.G.) alain.goossens@psb.vib-ugent.be.
authors: Alain
authors: 0000-0002-1599-551X
authors: A
authors: Goossens
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways; D003600:Cytosol / Q000378:metabolism*; D004249:DNA Damage; D018506:Gene Expression Regulation, Plant; D054477:Glutaredoxins / Q000235:genetics / Q000378:metabolism*; D015151:Immunoblotting; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D009154:Mutation; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014968:Xanthine Dehydrogenase / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C000604704:GRXS17 protein, Arabidopsis; D054477:Glutaredoxins; D007506:Iron-Sulfur Proteins; D014968:Xanthine Dehydrogenase