title: [Effects of expression of calpain mRNA in rabbits exposed to vibration by hind legs].
abstract: OBJECTIVE
  To study the effects of expression of calpain mRNA in rabbits exposed to vibration by hind legs.
  
  
  METHODS
  32 New Zealand rabbits were randomly divided into a control group and 3 experimental groups according to 4-hour energy-equivalent frequency-weighted acceleration[ahw (4)]: low (4.33 m/s(2)) , moderate (8.67 m/s(2)) and high (17.34 m/s(2)) intensity group to accepted the vibration by hind legs. 45 ds later, brain and skeletal muscle tissue of rabbits were taken to detect the expression of calpain-1 and calpain-2 mRNA by RT-qPCR technique.
  
  
  RESULTS
  The relative content of calpain-1 mRNA in the brain tissues in rabbits of low, medium and high intensity group were 8.35±3.75,9.64±4.54,5.10±5.26. While the relative content of calpain-2 mRNA in the brain tissues in rabbits of low, medium and high intensity group were 7.34±4.97,8.50±5.66, 8.16±5.59. Compared with the control group (1.10±0.29, 0.56±0.43) , the expression of calpain-1 and calpain-2 mRNA of the intensity groups showed an significantly increasing trend (P<0.01). In skeletal muscle tissue, the relative content of calpain-1 mRNA were 4.36±2.05, 7.37±4.06, 12.46±6.21.Compared with the control group (0.98±0.59) , the expression of calpain-1 mRNA of experiment groups were significantly higher (P<0.05) .The expression of calpain-2 mRNA of the intensity groups had no significantly difference with the control group (P>0.05).
  
  
  CONCLUSIONS
  The expression of calpain-1 and calpain-2 mRNA can be promoted by the vibration by the hind legs.
authors: Key Libratory of Occupational Health and Environmental Medicine, School of Public Health, Jining Medical University, Jining 272067, China.
authors: Z Q
authors: ZQ
authors: Zhang
authors: C Z
authors: CZ
authors: Zhang
authors: J C
authors: JC
authors: Nie
authors: L
authors: L
authors: Lin
mesh_terms: D000818:Animals; D001921:Brain; D002154:Calpain; D006614:Hindlimb; D018482:Muscle, Skeletal; D012333:RNA, Messenger; D011817:Rabbits; D014732:Vibration
chemical_list: D012333:RNA, Messenger; D002154:Calpain