title: The impact of diet on the composition and relative abundance of rumen microbes in goat.
abstract: OBJECTIVE
  This experiment was conducted to explore the impact of diet on the ruminal microbial community in goats.
  
  
  METHODS
  Twelve goats were divided into two groups and fed complete feed (CF) or all forage (AF) diet. The total microbial DNAs in the rumen liquid were extracted. The V4 region of microbial 16S rRNA genes was amplified and sequenced using high-throughput. Information of sequences was mainly analyzed by QIIME 1.8.0.
  
  
  RESULTS
  The results showed that Bacteroidetes and Firmicutes were the most predominant microbial phyla in the rumen of all goats. At genus level, the abundance of fiber-digesting bacteria such asRuminococcusandLachnospiracea incertae sediswas significantly higher in AF than that in CF, while the levels of fat-degrading bacteriumAnaerovibrioand protein-degrading bacteriumPseudomonaswere opposite. The core shared genera,PrevotellaandButyrivibriowere widespread in the rumen of goats and no significant difference was observed in relative abundance between groups.
  
  
  CONCLUSIONS
  We concluded that the richness of fiber-, protein-, and fat-digesting bacteria was affected by diet and tended to increase with the rise of their corresponding substrate contents in the ration; some bacteria shared by all goats maintained stable despite the difference in the ration, and they might be essential in maintaining the normal function of rumen.
authors: Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China.
authors: Kaizhen
authors: K
authors: Liu
authors: Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China.
authors: Qin
authors: Q
authors: Xu
authors: Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China.
authors: Lizhi
authors: L
authors: Wang
authors: Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China.
authors: Jiwen
authors: J
authors: Wang
authors: Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China.
authors: Wei
authors: W
authors: Guo
authors: Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China.
authors: Meili
authors: M
authors: Zhou
keywords: Diet; Diversity; Goat; High-throughput Sequencing; Rumen Microbe