title: High-sensitivity radioimmunoassay for substance P.
abstract: Optimisation of a non-equilibrium radioimmunoassay for substance P is described. The assay has a sensitivity of 2 pg (1.5 fmole), sufficient for substance P determinations in unexcepted human plasma and other biological material. The specificity of antisera used was tested with closely related peptides, physalaemin and eledoisin. Crossreaction of 1 per cent for eledoisin and 0.1 per cent for physalaemin was found. The assay is easy to perform, has a precision of 5 per cent, and takes only 24 hours.
authors: Endocrine Unit, Mater Misericordiae Hospital, Dublin 7.
authors: R
authors: R
authors: O'Connell
authors: Endocrine Unit, Mater Misericordiae Hospital, Dublin 7.
authors: P
authors: P
authors: Skrabanek
authors: Endocrine Unit, Mater Misericordiae Hospital, Dublin 7.
authors: D
authors: D
authors: Cannon
authors: Endocrine Unit, Mater Misericordiae Hospital, Dublin 7.
authors: D
authors: D
authors: Powell