title: Polyphenol-rich strawberry extract (PRSE) shows in vitro and in vivo biological activity against invasive breast cancer cells.
abstract: We describe the biological effects of a polyphenol-rich strawberry extract (PRSE), obtained from the "Alba" variety, on the highly aggressive and invasive basal-like breast cancer cell line A17. Dose-response and time-course experiments showed that PRSE is able to decrease the cellular viability of A17 cells in a time- and dose-dependent manner. PRSE effect on cell survival was investigated in other tumor and normal cell lines of both mouse and human origin, demonstrating that PRSE is more active against breast cancer cells. Cytofluorimetric analysis of A17 cells demonstrated that sub-lethal doses of PRSE reduce the number of cells in S phase, inducing the accumulation of cells in G1 phase of cell cycle. In addition, the migration of A17 cells was studied monitoring the ability of PRSE to inhibit cellular mobility. Gene expression analysis revealed the modulation of 12 genes playing different roles in the cellular migration, adhesion and invasion processes. Finally, in vivo experiments showed the growth inhibition of A17 cells orthotopically transplanted into FVB syngeneic mice fed with PRSE. Overall, we demonstrated that PRSE exerts important biological activities against a highly invasive breast cancer cell line both in vitro and in vivo suggesting the strawberry extracts as preventive/curative food strategy.
authors: Molecular Pathology Lab. "PaoLa", Dept. of Biomolecular Sciences, University of Urbino "Carlo Bo", Fano (PU), Italy.|Fondazione Umberto Veronesi, Milano, Italy.
authors: Stefano
authors: S
authors: Amatori
authors: Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche, Sez. Biochimica, Università Politecnica delle Marche, Ancona, Italy.|Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, Ancona, Italy.
authors: Luca
authors: L
authors: Mazzoni
authors: Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche, Sez. Biochimica, Università Politecnica delle Marche, Ancona, Italy.|Escuela de Medicina Veterinaria y Zootecnia, Facultad de Ciencias de la Salud, Universidad de Las Américas (UDLA), Quito, Ecuador.
authors: Josè Miguel
authors: JM
authors: Alvarez-Suarez
authors: Fondazione Umberto Veronesi, Milano, Italy.|Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche, Sez. Biochimica, Università Politecnica delle Marche, Ancona, Italy.
authors: Francesca
authors: F
authors: Giampieri
authors: Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche, Sez. Biochimica, Università Politecnica delle Marche, Ancona, Italy.
authors: Massimiliano
authors: M
authors: Gasparrini
authors: Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche, Sez. Biochimica, Università Politecnica delle Marche, Ancona, Italy.|Area de Nutrición y Salud, Universidad Internacional Iberoamericana (UNINI), Campeche, C.P.24040, Mexico.
authors: Tamara Yuliett
authors: TY
authors: Forbes-Hernandez
authors: Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche, Sez. Biochimica, Università Politecnica delle Marche, Ancona, Italy.
authors: Sadia
authors: S
authors: Afrin
authors: Molecular Pathology Lab. "PaoLa", Dept. of Biomolecular Sciences, University of Urbino "Carlo Bo", Fano (PU), Italy.
authors: Alfredo
authors: A
authors: Errico Provenzano
authors: Molecular Pathology Lab. "PaoLa", Dept. of Biomolecular Sciences, University of Urbino "Carlo Bo", Fano (PU), Italy.
authors: Giuseppe
authors: G
authors: Persico
authors: Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, Ancona, Italy.
authors: Bruno
authors: B
authors: Mezzetti
authors: Scuola di Bioscienze e Medicina Veterinaria, Università di Camerino, Camerino, Italy.
authors: Augusto
authors: A
authors: Amici
authors: Molecular Pathology Lab. "PaoLa", Dept. of Biomolecular Sciences, University of Urbino "Carlo Bo", Fano (PU), Italy.
authors: Mirco
authors: M
authors: Fanelli
authors: Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche, Sez. Biochimica, Università Politecnica delle Marche, Ancona, Italy.|Centre for Nutrition &Health, Universidad Europea del Atlantico (UEA), Santander, Spain.
authors: Maurizio
authors: M
authors: Battino
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D031985:Fragaria / Q000737:chemistry*; D020869:Gene Expression Profiling; D064593:Heterografts; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D059808:Polyphenols