title: Treatment of chronic ulcer in diabetic rats with self assembling nanofiber gel encapsulated-polydeoxyribonucleotide.
abstract: OBJECTIVE
  This study aims to explore the treatment effects of chronic ulcer in diabetic rats with self assembling nanofiber gel encapsulated-polydeoxyribonucleotide.
  
  
  METHODS
  Diabetic skin ulcer mouse model was established in this study. They were divided into control group, common wound group and infectious wound group. Human embryonic fibroblast cells and vascular endothelial cells were treated with short poly-N-acetyl glucosamine nanofibers and polydeoxyribonucleotide. Their effects on cell proliferation, revascularization and inhibiting infection were detected by RT-PCR, western-blotting, HE staining and immunohistochemical methods respectively.
  
  
  RESULTS
  The expression levels of cytokines and angiogenic factors increased in the treatment groups especially in sNAG encapsulated-PDRN group. HE staining results indicated that PDRN, sNAG and sNAG encapsulated-PDRN could improve the wound healing, immunohistochemical results showed that PDRN, sNAG and sNAG encapsulated-PDRN promoted cell proliferation and new vessel formation especially sNAG encapsulated-PDRN.
  
  
  CONCLUSIONS
  sNAG encapsulated-PDRN may have a potential application in the treatment of diabetic ulcers and chronic wound healing.
authors: Department of Orthopedics, Union Hospital, Huazhong University of Science and Technology 1277 Jiefang Road, Wuhan, Hubei 430022, China.
authors: Xi
authors: X
authors: Chen
authors: Department of Orthopedics, Union Hospital, Huazhong University of Science and Technology 1277 Jiefang Road, Wuhan, Hubei 430022, China.
authors: Wu
authors: W
authors: Zhou
authors: Department of Orthopedics, Union Hospital, Huazhong University of Science and Technology 1277 Jiefang Road, Wuhan, Hubei 430022, China.
authors: Kun
authors: K
authors: Zha
authors: Department of Orthopedics, Union Hospital, Huazhong University of Science and Technology 1277 Jiefang Road, Wuhan, Hubei 430022, China.
authors: Guohui
authors: G
authors: Liu
authors: Department of Orthopedics, Union Hospital, Huazhong University of Science and Technology 1277 Jiefang Road, Wuhan, Hubei 430022, China.
authors: Shuhua
authors: S
authors: Yang
authors: Department of Orthopedics, Union Hospital, Huazhong University of Science and Technology 1277 Jiefang Road, Wuhan, Hubei 430022, China.
authors: Shunan
authors: S
authors: Ye
authors: Department of Orthopedics, Union Hospital, Huazhong University of Science and Technology 1277 Jiefang Road, Wuhan, Hubei 430022, China.
authors: Yi
authors: Y
authors: Liu
authors: Department of Orthopedics, Union Hospital, Huazhong University of Science and Technology 1277 Jiefang Road, Wuhan, Hubei 430022, China.
authors: Yuan
authors: Y
authors: Xiong
authors: Department of Orthopedics, Union Hospital, Huazhong University of Science and Technology 1277 Jiefang Road, Wuhan, Hubei 430022, China.
authors: Yongchao
authors: Y
authors: Wu
authors: Department of Orthopedics, Union Hospital, Huazhong University of Science and Technology 1277 Jiefang Road, Wuhan, Hubei 430022, China.
authors: Faqi
authors: F
authors: Cao
keywords: Nanofibers; chronic ulcer; cytokines; diabetes; poly-N-acetyl glucosamine; polydeoxyribonucleotide; wound healing