title: Novel nanocomposites with selective antibacterial action and low cytotoxic effect on eukaryotic cells.
abstract: In the present study we synthesized lignin-tetra ethoxysilane (TEOS) nanocomposite and characterized it using UV-spectroscopy, Fourier Transform Infra-red spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Field Emission-Scanning Electron Microscopy (FE-SEM) and Scanning Electron Microscopy (SEM). XRD spectra and SEM micrographs confirmed a relatively high degree of crystallinity (peaks located at lower angle, 2θ=12° and 2θ=22.0°) and porous nature of nanocomposite. The lignin-TEOS nanocomposites depicted antibacterial activity against the test microorganisms (Pseudomonas aerugenosa MTCC 741, Escherichia coli MTCC 739, Bacillus subtilis MTCC 441 and Staphylococcus aureus MTCC 96) whereas at the same concentration did not show any significant cytotoxicity against various tissue-specific cancer cell lines such as breast cancer: MCF-7, MDA-MB-231, MDA-MB-468, BT-549; lung cancer: A-549; prostate cancer: PC-3, Du-145; as well as primary control cells-Human hepatic stellate cells (HHSteCs). The present study suggests the plausible translational role of these nanocomposites as an antimicrobial agent for wound dressings due to its potent antimicrobial activity with low toxicity to non-target eukaryotic cells. Nevertheless, these nanocomposites may also be used as packaging materials due to their antimicrobial activity.
authors: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur 49500, Chhattisgarh, India.
authors: Kumari
authors: K
authors: Shweta
authors: Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad 500 007, TS, India.
authors: Kanakaraju
authors: K
authors: Manupati
authors: Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad 500 007, TS, India. Electronic address: amitavadas@iict.res.in.
authors: Amitava
authors: A
authors: Das
authors: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur 49500, Chhattisgarh, India. Electronic address: haritjha74@gmail.com.
authors: Harit
authors: H
authors: Jha
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D005057:Eukaryotic Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D008031:Lignin / Q000138:chemical synthesis; D008826:Microbial Sensitivity Tests; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D012821:Silanes / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D012821:Silanes; C522569:triethoxysilane; D008031:Lignin
keywords: Agglomeration; Antimicrobial activity; Cytotoxicity; Lignin-TEOS nanocomposites; Spectral study