title: miR-145 suppresses breast cancer cell migration by targeting FSCN-1 and inhibiting epithelial-mesenchymal transition.
abstract: MicroRNAs (miRNAs), small non-coding RNAs, regulate fundamental cellular and developmental processes such as cell growth, apoptosis, migration, and invasion. In our present study, we investigated the inhibitory role of miR-145 on breast cancer cell migration as well as its underlying mechanism. Wound healing assay and transwell migration assay showed that ectopic expression of miR-145 significantly inhibited breast cancer cell migration. Bioinformatics analysis revealed that FSCN-1 was a putative target of miR-145. The expression of FSCN-1 varied among four different breast cancer cells, and inversely correlated with miR-145 levels. Moreover, miR-145 mimic transfection enhanced the expression of FSCN-1 in Bcap-37 and HCC-1937 cells. We also found that siRNA- mediated down-regulation of FSCN-1 inhibited cell motility in breast cancer cells. In addition, we found that up-regulation of miR-145 blocked EMT and decreased the expression of MMP-2/9 in breast cancer cells. These results reveal a new link between miR-145, FSCN-1 and EMT in the regulation of breast cancer migration.
authors: Department of Breast Surgery, First Affiliated Hospital of Zhejiang Chinese Medical University Youdian Road 54#, Shangcheng District, Hangzhou City 310006, Zhejiang Province, China.
authors: Hong
authors: H
authors: Zhao
authors: Department of General Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing 210008, Jiangsu Province, China.
authors: Xing
authors: X
authors: Kang
authors: Department of General Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing 210008, Jiangsu Province, China.
authors: Xuefeng
authors: X
authors: Xia
authors: Department of Breast Surgery, First Affiliated Hospital of Zhejiang Chinese Medical University Youdian Road 54#, Shangcheng District, Hangzhou City 310006, Zhejiang Province, China.
authors: Like
authors: L
authors: Wo
authors: Department of Breast Surgery, First Affiliated Hospital of Zhejiang Chinese Medical University Youdian Road 54#, Shangcheng District, Hangzhou City 310006, Zhejiang Province, China.
authors: Xidong
authors: X
authors: Gu
authors: Department of Breast Surgery, First Affiliated Hospital of Zhejiang Chinese Medical University Youdian Road 54#, Shangcheng District, Hangzhou City 310006, Zhejiang Province, China.
authors: Yuanyuan
authors: Y
authors: Hu
authors: Department of Breast Surgery, First Affiliated Hospital of Zhejiang Chinese Medical University Youdian Road 54#, Shangcheng District, Hangzhou City 310006, Zhejiang Province, China.
authors: Xiaohong
authors: X
authors: Xie
authors: Department of Surgery, Revlon/UCLA Breast Canter, David Geffen School of Medicine Los Angeles, CA, USA.
authors: Helena
authors: H
authors: Chang
authors: Department of Breast Surgery, First Affiliated Hospital of Zhejiang Chinese Medical University Youdian Road 54#, Shangcheng District, Hangzhou City 310006, Zhejiang Province, China.
authors: Lihua
authors: L
authors: Lou
authors: Department of Surgery, The First Hospital of Jiaxing 1882 Zhong'huan'nan Road, Jiaxing 314000, Zhejiang Province, China.
authors: Xuning
authors: X
authors: Shen
keywords: Breast cancer; FSCN-1; epithelial-mesenchymal transition; miR-145; migration