title: Horseradish peroxidase supported on porous graphene as a novel sensing platform for detection of hydrogen peroxide in living cells sensitively.
abstract: A viable and simple method for preparing porous graphene network using silver nanoparticles (AgNPs) etching was proposed, and a sensitive biosensor was constructed based on the porous graphene (PGN) and horseradish peroxidase (HRP) to measure the release of H2O2from living cells. Owing to the large surface area and versatile porous structure, the use of nanoporous materials can significantly improve the analysis performance of the biosensor by loading large amounts of enzyme and accelerating diffusion rate. Meanwhile, the constructed electrode exhibited excellent electrochemical performance toward H2O2with a determination limit as low as 0.0267nM and wide linear range of 7 orders of magnitude, which was superior to other H2O2electrochemical sensors. Thus, this novel biosensor can detect the H2O2release from living cells not only under normal physiological conditions (10-8-10-7M) but also in emergency state with the increased concentration (~10-4M). This work provides tremendous potential for real-time tracking of the secretion of H2O2in different types of physiological and pathological investigations.
authors: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
authors: Yidan
authors: Y
authors: Liu
authors: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China. Electronic address: liuxh@nwnu.edu.cn.
authors: Xiuhui
authors: X
authors: Liu
authors: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
authors: Zhipan
authors: Z
authors: Guo
authors: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
authors: Zhongai
authors: Z
authors: Hu
authors: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
authors: Zhonghua
authors: Z
authors: Xue
authors: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China. Electronic address: luxq@nwnu.edu.cn.
authors: Xiaoquan
authors: X
authors: Lu
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002470:Cell Survival; D055664:Electrochemical Techniques / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D016716:PC12 Cells; D016062:Porosity; D051381:Rats; D012834:Silver / Q000737:chemistry*
chemical_list: D012834:Silver; D006108:Graphite; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase
keywords: Cell tracking; Electrochemical biosensor; Horseradish peroxidase; Hydrogen peroxide detection; Porous graphene