title: In vitro studies on interaction of rat pancreatic lipase and colipase with biliary lipids.
abstract: Lipase and colipase were prepared separately from rat pancreatic juice, and their respective interaction with biliary lipids was investigated by gel filtration on agarose in the presence of a micellar solution of sodium taurocholate. It was found that the cofactor can associate with the biliary lipids, whereas the enzyme forms a high mol wt complex only in the presence of colipase. It is suggested that biliary phospholipids might participate in the in vivo formation of the enzyme-cofactor substrate complex at the triglyceride-water interface in the presence of bile salts.
authors: Groupe de Recherches sur le Transport des Lipides U130-INSERM, 13009, Marseille, France.
authors: D
authors: D
authors: Lairon
authors: Groupe de Recherches sur le Transport des Lipides U130-INSERM, 13009, Marseille, France.
authors: G
authors: G
authors: Nalbone
authors: Groupe de Recherches sur le Transport des Lipides U130-INSERM, 13009, Marseille, France.
authors: N
authors: N
authors: Domingo
authors: Groupe de Recherches sur le Transport des Lipides U130-INSERM, 13009, Marseille, France.
authors: H
authors: H
authors: Lafont
authors: Groupe de Recherches sur le Transport des Lipides U130-INSERM, 13009, Marseille, France.
authors: J
authors: J
authors: Hauton
authors: Institute de Chimie Biologique, Faculté St-Charles, 13331, Marseille Cedex 3, France.
authors: R
authors: R
authors: Julien
authors: Institute de Chimie Biologique, Faculté St-Charles, 13331, Marseille Cedex 3, France.
authors: J
authors: J
authors: Rathelot
authors: Institute de Chimie Biologique, Faculté St-Charles, 13331, Marseille Cedex 3, France.
authors: P
authors: P
authors: Canioni
authors: Institute de Chimie Biologique, Faculté St-Charles, 13331, Marseille Cedex 3, France.
authors: L
authors: L
authors: Sarda