title: Further development on DMFC device used for analytical purpose: real applications in the pharmaceutical field and possible in biological fluids.
abstract: The analytical research devoted to the utilization of the direct methanol fuel cell (DMFC) for analytical purposes has been continued. The research reported in this paper concerns two points, one of which was the possibility of improving the features, from the analytical point of view, of a catalytic fuel cell for methanol and ethanol, by introducing an enzyme, immobilized into a dialysis membrane small bag, in the anodic area of the fuel cell. This objective has been fully achieved, particularly using the enzyme alcohol dehydrogenase, which has increased the sensitivity of the method and reduced dramatically the response time of the cell. The second point concerned the opportunity to determine two particular antibiotics having an alcohol functional group in their molecule, that is, imipenem and chloramphenicol. Also, this goal has been reached, even if the sensitivity of the method is not so high. Graphical abstract Imipenem and Chloramphenicol determination using the DMFC and Ethanol determination using the enzymatic DMFC.
authors: Department of Chemistry, University of Rome "La Sapienza", Piazzale Aldo Moro, 5, 00185, Rome, Italy. mauro.tomassetti@uniroma1.it.
authors: Mauro
authors: M
authors: Tomassetti
authors: Department of Chemistry, University of Rome "La Sapienza", Piazzale Aldo Moro, 5, 00185, Rome, Italy.
authors: Giovanni
authors: G
authors: Merola
authors: Department of Chemistry, University of Rome "La Sapienza", Piazzale Aldo Moro, 5, 00185, Rome, Italy.
authors: Riccardo
authors: R
authors: Angeloni
authors: Department of Chemistry, University of Rome "La Sapienza", Piazzale Aldo Moro, 5, 00185, Rome, Italy.
authors: Sergio
authors: S
authors: Marchiandi
authors: Department of Chemistry, University of Rome "La Sapienza", Piazzale Aldo Moro, 5, 00185, Rome, Italy.
authors: Luigi
authors: L
authors: Campanella
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001674:Bioelectric Energy Sources / Q000382:microbiology; D002701:Chloramphenicol / Q000032:analysis*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D000431:Ethanol / Q000032:analysis; D015378:Imipenem / Q000032:analysis*; D008567:Membranes, Artificial; D000432:Methanol / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D000900:Anti-Bacterial Agents; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D000431:Ethanol; D002701:Chloramphenicol; D015378:Imipenem; D000426:Alcohol Dehydrogenase; D000432:Methanol
keywords: Direct catalytic fuel cell; Imipenem and chloramphenicol determination; Immobilized enzyme; Improvement performances