title: 2,3,5,4'-Tetrahydroxystilbene-2-O-β-glucoside Isolated from Polygoni Multiflori Ameliorates the Development of Periodontitis.
abstract: Periodontitis, a chronic infection by periodontopathic bacteria, induces uncontrolled inflammation, which leads to periodontal tissue destruction. 2,3,5,4'-Tetrahydroxystilbene-2-O-beta-glucoside (THSG), a polyphenol extracted from Polygoni Multiflori, reportedly has anti-inflammatory properties. In this study, we investigated the mechanisms of THSG on the Porphyromonas gingivalis-induced inflammatory responses in human gingival fibroblasts and animal modeling of ligature-induced periodontitis. Human gingival fibroblast cells were treated with lipopolysaccharide (LPS) extracted from P. gingivalis in the presence of resveratrol or THSG to analyze the expression of TNF-α, IL-1β, and IL-6 genes. Increased AMP-activated protein kinase (AMPK) activation and SirT1 expression were induced by THSG. Treatment of THSG decreased the expression of LPS-induced inflammatory cytokines, enhanced AMPK activation, and increased the expression of SirT1. In addition, it suppressed the activation of NF-κB when cells were stimulated with P. gingivalis LPS. The anti-inflammatory effect of THSG and P. Multiflori crude extracts was reproduced in ligature-induced periodontitis animal modeling. In conclusion, THSG inhibited the inflammatory responses of P. gingivalis-stimulated human gingival fibroblasts and ameliorated ligature-induced periodontitis in animal model.
authors: Taipei Cancer Center, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan; Department of Dentistry, Wan-Fang Medical Center, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors: Yu-Tang
authors: YT
authors: Chin
authors: PhD Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors: Meng-Ti
authors: MT
authors: Hsieh
authors: Taipei Cancer Center, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan; Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan.
authors: Chi-Yu
authors: CY
authors: Lin
authors: Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.
authors: Po-Jan
authors: PJ
authors: Kuo
authors: Joint Biobank, Office of Human Research, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors: Yu-Chen S H
authors: YC
authors: Yang
authors: Taipei Cancer Center, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors: Ya-Jung
authors: YJ
authors: Shih
authors: Taipei Cancer Center, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors: Hsuan-Yu
authors: HY
authors: Lai
authors: PhD Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors: Guei-Yun
authors: GY
authors: Cheng
authors: Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Albany, NY, USA.
authors: Heng-Yuan
authors: HY
authors: Tang
authors: Department of Microbiology and Immunology, School of Medicine, China Medicine University, Taichung, Taiwan.
authors: Chen-Chen
authors: CC
authors: Lee
authors: Department of Dentistry, Wan-Fang Medical Center, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan; School of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors: Sheng-Yang
authors: SY
authors: Lee
authors: School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors: Ching-Chiung
authors: CC
authors: Wang
authors: Taipei Cancer Center, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan; PhD Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors: Hung-Yun
authors: 0000-0003-2824-6419
authors: HY
authors: Lin
authors: Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan.
authors: Earl
authors: 0000-0003-0360-3643
authors: E
authors: Fu
authors: Taipei Cancer Center, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors: Jacqueline
authors: J
authors: Whang-Peng
authors: Taipei Cancer Center, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors: Leroy F
authors: LF
authors: Liu
mesh_terms: D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000166:cytology* / Q000187:drug effects / Q000473:pathology; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D010518:Periodontitis / Q000188:drug therapy*; D019664:Polygonaceae / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D055815:Young Adult
chemical_list: C489707:2',3',4',5'-tetrahydroxystilbene-2-O-beta-D-glucoside; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D013267:Stilbenes