title: Effect of insulin-like growth factor-1 and hyaluronic acid in experimentally produced osteochondral defects in rats.
abstract: BACKGROUND
  The common purpose of almost all methods used to treat the osteochondral injuries is to produce a normal cartilage matrix. However current methods are not sufficient to provide a normal cartilage matrix. For that reason, researchers have studied to increase the effectiveness of this methods using chondrogenic and chondroprotective molecules in recent experimental studies. Insulin-like growth factor-1 (IGF-1) and hyaluronic acid (HA) are two important agents used in this field. This study compared the effects of IGF-1 and HA in an experimental osteochondral defect in rat femora.
  
  
  METHODS
  The rats were divided into three groups (n = 15 per group) as follows: The IGF-1 group, HA group, and control group. An osteochondral defect of a diameter of 1.5 mm and a depth of 2 mm was created on the patellar joint side of femoral condyles. The IGF-1 group received an absorbable gelatin sponge soaked with 15 μg/15 μl of IGF-1, and the HA group received an absorbable gelatin sponge soaked with 80 μg HA. The control group received only an absorbable gelatin sponge. Rats were sacrificed at the 6(th) week, and the femur condyles were evaluated histologically.
  
  
  RESULTS
  According to the total Mankin scale, there was a statistically significant difference between IGF-1 and HA groups and between IGF-1 and control groups. There was also a significant statistical difference between HA and control groups.
  
  
  CONCLUSIONS
  It was shown histopathologically that IGF-1 is an effective molecule for osteochondral lesions. Although it is weaker than IGF-1, HA also strengthened the repair tissue.
authors: Department of Orthopaedics, Dicle University Medical Faculty, Diyarbakir, Turkey.
authors: Celil
authors: C
authors: Alemdar
authors: Department of Orthopaedics, Düzce University Medical Faculty, Düzce, Turkey.
authors: İstemi
authors: İ
authors: Yücel
authors: Department of Orthopaedics, Tekirdağ State Hospital, Tekirdağ, Turkey.
authors: Barış
authors: B
authors: Erbil
authors: Department of Pathology, Ordu University Medical Faculty, Ordu, Turkey.
authors: Havva
authors: H
authors: Erdem
authors: Department of Orthopaedics, Dicle University Medical Faculty, Diyarbakir, Turkey.
authors: Ramazan
authors: R
authors: Atiç
authors: Department of Orthopaedics, Dicle University Medical Faculty, Diyarbakir, Turkey.
authors: Emin
authors: E
authors: Özkul
keywords: Cartilage; Hyaluronic acid; IGF-1; cartilage; hyaluronic acid; insulin-like growth factor-1; osteochondral defect; rats; sports medicine