title: Pilot Metabolome-Wide Association Study of Benzo(a)pyrene in Serum From Military Personnel.
abstract: A pilot study was conducted to test the feasibility of using Department of Defense Serum Repository (DoDSR) samples to study health and exposure-related effects.
  
  
  
  Thirty unidentified human serum samples were obtained from the DoDSR and analyzed for normal serum metabolites with high-resolution mass spectrometry and serum levels of free benzo(a)pyrene (BaP) by gas chromatography-mass spectrometry. Metabolic associations with BaP were determined using a metabolome-wide association study (MWAS) and metabolic pathway enrichment.
  
  
  
  The serum analysis detected normal ranges of glucose, selected amino acids, fatty acids, and creatinine. Free BaP was detected in a broad concentration range. MWAS of BaP showed associations with lipids, fatty acids, and sulfur amino acid metabolic pathways.
  
  
  
  The results show that the DoDSR samples are of sufficient quality for chemical profiling of DoD personnel.
authors: Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Emory University, Atlanta, Georgia (Mr Walker, Drs Uppal and Jones); Department of Civil and Environmental Engineering, Tufts University, Medford, Massachusetts (Mr Walker, Dr Pennell); Center for Air Resources Engineering and Science, Clarkson University, Potsdam (Drs Xia, Hopke); Pulmonary Division, Department of Medicine (Drs Utell, Phipps, Sime), Department of Environmental Medicine, University of Rochester Medical Center, New York (Drs Utell, Phipps, Sime); 779th Aerospace Medicine Squadron, 79th Medical Wing, Joint Base Andrews, MD (Dr Rohrbeck); and Professor, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814 (Dr Mallon).
authors: Douglas I
authors: DI
authors: Walker
authors: Kurt D
authors: KD
authors: Pennell
authors: Karan
authors: K
authors: Uppal
authors: Xiaoyan
authors: X
authors: Xia
authors: Philip K
authors: PK
authors: Hopke
authors: Mark J
authors: MJ
authors: Utell
authors: Richard P
authors: RP
authors: Phipps
authors: Patricia J
authors: PJ
authors: Sime
authors: Patricia
authors: P
authors: Rohrbeck
authors: Col Timothy M
authors: CT
authors: Mallon
authors: Dean P
authors: DP
authors: Jones
mesh_terms: D001564:Benzo(a)pyrene / Q000032:analysis*; D001771:Blood Banks; D004781:Environmental Exposure / Q000009:adverse effects; D006801:Humans; D013058:Mass Spectrometry; D055442:Metabolome; D008889:Military Personnel; D016273:Occupational Exposure / Q000009:adverse effects; D010865:Pilot Projects; D044967:Serum / Q000737:chemistry*
chemical_list: D001564:Benzo(a)pyrene