title: Influence of HLA-DR polymorphism and allergic sensitization on humoral immune responses to intact pneumococcus in a transgenic mouse model.
abstract: Asthma is independently associated with HLA-DR3 and increased risks of pneumococcal diseases. We aimed to determine whether HLA-DR polymorphism (HLA-DRB1*03), sensitization to house dust mite (HDM), or their interaction affects humoral immune responses to pneumococcal polysaccharide and protein antigens of intact pneumococci. Induction of serum titers of anti-pneumococcal polysaccharide and anti-surface protein IgM and IgG in response to immunization with intact pneumococci (Pn) serotype 14 was determined using humanized HLA-DR3 and DR2 transgenic mice. Transgenic mice were sensitized by injecting HDM and challenged with intranasal HDM. Mice were subsequently immunized with heat-killed Pn14 at day 24. Serum titers of anti-phosphorylcholine (PC) IgM and IgG, anti-pneumococcal polysaccharide, capsular type 14 (PPS14) IgM and IgG, and anti-pneumococcal surface protein A (PspA) IgG were measured. We included a total of 44 mice (22 DR3 and 22 DR2 mice) and half of mice in each group were sensitized with HDM (i.e. 22 HDM-sensitized and 22 control mice). HDM-sensitized mice, irrespective of HLA-DR polymorphism, had significantly lower humoral immune responses. HLA-DR3 mice, irrespective of HDM sensitization, elicited a significantly lower anti-PC IgG response. In contrast, the anti-PspA IgG response was higher in DR3 relative to DR2 mice. The effect of HDM sensitization on lowering humoral immune responses to Pn14 was observed in DR3 mice regardless of the nature of the antigen, whereas such decreases were observed only for the anti-PPS14 IgG and anti-PC IgM responses in DR2 mice. HDM sensitization lowered humoral immune responses to intact pneumococcus and this effect was significantly modified by the HLA-DR polymorphism.
authors: Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester, MN, USA.|Department of Pediatrics, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul, Korea.
authors: Y H
authors: YH
authors: Sheen
authors: Department of Immunology, Mayo Clinic, Rochester, MN, USA.
authors: G
authors: G
authors: Rajagopalan
authors: Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
authors: C M
authors: CM
authors: Snapper
authors: Department of Internal Medicine, Mayo Clinic, Rochester, MN, USA.
authors: H
authors: H
authors: Kita
authors: Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester, MN, USA.
authors: C-I
authors: CI
authors: Wi
authors: Children's Hospital Primary Care Center, Boston Children's Hospital, Boston, MA, USA.
authors: P J
authors: PJ
authors: Umaretiya
authors: Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester, MN, USA.|Department of Internal Medicine, Mayo Clinic, Rochester, MN, USA.
authors: Y J
authors: YJ
authors: Juhn
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology; D015802:HLA-DR2 Antigen / Q000235:genetics / Q000276:immunology*; D015803:HLA-DR3 Antigen / Q000235:genetics / Q000276:immunology*; D059811:HLA-DRB1 Chains / Q000235:genetics / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D056724:Immunity, Humoral / Q000187:drug effects*; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D051379:Mice; D008822:Mice, Transgenic; D011110:Polymorphism, Genetic; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage / Q000276:immunology; D039981:Pyroglyphidae / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D015802:HLA-DR2 Antigen; D015803:HLA-DR3 Antigen; D059811:HLA-DRB1 Chains; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011135:Polysaccharides, Bacterial; C051592:pneumococcal surface protein A
keywords: HLA-DR3; Streptococcus pneumoniae; adaptive immunity; sensitization; transgenic mice