title: miRNA143 Induces K562 Cell Apoptosis Through Downregulating BCR-ABL.
abstract: BACKGROUND Leukemia seriously threats human health and life. MicroRNA regulates cell growth, proliferation, apoptosis, and cell cycle. Whether microRNA could be treated as a target for leukemia is still unclear and the mechanism by which microRNA143 regulates K562 cells needs further investigation. MATERIAL AND METHODS miRNA143 and its scramble miRNA were synthesized and transfected to K562 cells. MTT assay was used to detect K562 cell proliferation. Flow cytometry and a caspase-3 activity detection kit were used to test K562 cell apoptosis. Western blot analysis was performed to determine breakpoint cluster region-Abelson (BCR-ABL) expression. BCR-ABL overexpression and siRNA were used to change BCR-ABL level, and cell apoptosis was detected again after lipofection transfection. RESULTS miRNA143 transfection inhibited K562 cell growth and induced its apoptosis. miRNA143 transfection decreased BCR-ABL expression. BCR-ABL overexpression suppressed miRNA143-induced K562 cell apoptosis, while its reduction enhanced miRNA143-induced apoptosis. CONCLUSIONS miRNA143 induced K562 cell apoptosis through downregulating BCR-ABL. miRNA143 might be a target for a new leukemia therapy.
authors: Institute of Genetic Engineering, Southern Medical University, Baiyun, Guangzhou, China (mainland).
authors: Bing
authors: B
authors: Liang
authors: Institute of Genetic Engineering, Southern Medical University, Baiyun, Guangzhou, China (mainland).
authors: Yanbin
authors: Y
authors: Song
authors: Institute of Genetic Engineering, Southern Medical University, Baiyun, Guangzhou, China (mainland).
authors: Wenling
authors: W
authors: Zheng
authors: Institute of Genetic Engineering, Southern Medical University, Baiyun, Guangzhou, China (mainland).
authors: Wenli
authors: W
authors: Ma
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D016313:Genes, abl / Q000235:genetics*; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000628:therapy; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: C542359:MIRN143 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering