title: Quantitation of anticancer drugs - Cyclophosphamide and ifosfamide in urine and water sewage samples by gas chromatography-mass spectrometry.
abstract: OBJECTIVE
  Cyclophosphamide (CP) and ifosfamide (IF) are effective anti-cancer drugs but their genotoxicity can harm everyone contacting them occupationally or environmentally. Therefore, a sensitive method for monitoring their amounts in biological and environmental samples is needed. This has aimed to develop a method for analyzing these drugs in urine and water sewage samples.
  
  
  METHODS
  The drug spiked samples were extracted, derivatized, and analyzed by gas chromatography-mass spectrometry and the analytical parameters were validated.
  
  
  RESULTS
  The method gave linear calibration curves at the concentrations of 0-190 nmol/l. It had the quantitation limit of 3.8 nmol/l and showed acceptable specificity, accuracy, recovery and precision.
  
  
  CONCLUSIONS
  The developed method can be used reliably for monitoring CP and IF concentrations in urine and water sewage. The method will be applied for preventing health risk from occupational and environmental exposures to these drugs. Int J Occup Med Environ Health 2016;29(5):815-822.
authors: Srinakharinwirot University, Bangkok, Thailand (Faculty of Medicine, Department of Pathology). veeravah@swu.ac.th.
authors: Veeravan
authors: V
authors: Lekskulchai
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis* / Q000652:urine; D003520:Cyclophosphamide / Q000032:analysis* / Q000652:urine; D004784:Environmental Monitoring / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007069:Ifosfamide / Q000032:analysis* / Q000652:urine; D012722:Sewage / Q000032:analysis*
chemical_list: D000970:Antineoplastic Agents; D012722:Sewage; D003520:Cyclophosphamide; D007069:Ifosfamide
keywords: antineoplastic agents; cyclophosphamide; environment; gas chromatography–mass spectrometry; ifosfamide; sensitive and specificity