title: Data on biochemical fluxes generated from biofabricated enzyme complexes assembled through engineered tags and microbial transglutaminase.
abstract: Data presented is related to an article titled "Modular construction of multi-subunit protein complexes using engineered tags and microbial transglutaminase" (Bhokisham et al., 2016) [1]. In this article, we have presented western blot and flux data associated with assembly of Pfs-LuxS enzyme complexes on beads using uni-tagged and bi-tagged LuxS enzymes. We have also presented biochemical flux following changes in enzyme stoichiometries. We covalently coupled a Pfs-LuxS complex with Protein G, an antibody binding non-enzyme component and directed these complexes to the surfaces of bacterial cells via anti-Escherichia coli antibodies. Fluorescence microscopy images represented the altered behavior of bacterial cells in response to the autoinducer-2 that is synthesized by the Protein G-enzyme complexes.
authors: Biological Sciences Graduate Program - College of Computer, Mathematical and Natural Sciences, University of Maryland, 4066 Campus Drive, College Park, MD 20742, United States; Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States.
authors: Narendranath
authors: N
authors: Bhokisham
authors: Fischell Department of Bioengineering, University of Maryland, Room 3122, Jeong H. Kim Engineering Building (Bldg. #225), College Park, MD 20742, United States.
authors: Haig
authors: H
authors: Pakhchanian
authors: Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States; Fischell Department of Bioengineering, University of Maryland, Room 3122, Jeong H. Kim Engineering Building (Bldg. #225), College Park, MD 20742, United States.
authors: David
authors: D
authors: Quan
authors: Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States; Fischell Department of Bioengineering, University of Maryland, Room 3122, Jeong H. Kim Engineering Building (Bldg. #225), College Park, MD 20742, United States.
authors: Tanya
authors: T
authors: Tschirhart
authors: Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States; Fischell Department of Bioengineering, University of Maryland, Room 3122, Jeong H. Kim Engineering Building (Bldg. #225), College Park, MD 20742, United States.
authors: Chen-Yu
authors: CY
authors: Tsao
authors: Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States; Fischell Department of Bioengineering, University of Maryland, Room 3122, Jeong H. Kim Engineering Building (Bldg. #225), College Park, MD 20742, United States.
authors: Gregory F
authors: GF
authors: Payne
authors: Biological Sciences Graduate Program - College of Computer, Mathematical and Natural Sciences, University of Maryland, 4066 Campus Drive, College Park, MD 20742, United States; Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States; Fischell Department of Bioengineering, University of Maryland, Room 3122, Jeong H. Kim Engineering Building (Bldg. #225), College Park, MD 20742, United States.
authors: William E
authors: WE
authors: Bentley
keywords: Biofabrication; Engineered tags; Metabolic flux; Quorum sensing; Transglutaminase