title: Investigate electrochemical immunosensor of cortisol based on gold nanoparticles/magnetic functionalized reduced graphene oxide.
abstract: A sensitively competitive electrochemical immunosensor for the detection of cortisol was successfully developed based on gold nanoparticles and magnetic functionalized reduced graphene oxide (AuNPs/MrGO). In order to construct the base of the immunosensor, the MrGO was initially fabricated by chemical cross-linking and used to modify the nafion pretreated glassy carbon electrode. Subsequently, the surface of electrode was modified by AuNPs via electrochemical deposition. A variety of cortisol (Cor) can be firmly loaded in the AuNPs/MrGO with large specific surface area and good bioactivity to construct the basic electrode (Cor/AuNPs/MrGO/Nafion@GCE), which was characterized by the cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), respectively. Due to the cortisol on the surface of basic electrode and samples can competitively combine with the cortisol antibody labelled by horseradish peroxidase (HRP-Strept-Biotin-Ab). Finally, the detection signal of electrochemical immunosensor (HRP-Strept-Biotin-Ab-Cor/AuNPs/MrGO/Nafion@GCE) in the test liquid had negative correlations with the concentration of cortisol in samples. The AuNPs/MrGO with excellent electrical conductivity being applied, the electrochemical response of the immunosensor was immensely amplified. The immunosensor displayed excellent analytical performance for the detection of cortisol range from 0.1 to 1000ng/mL with a detection limit of 0.05ng/mL at 3σ. Moreover, compared the developed immunoassay with commercially available enzyme linked immunosorbent assay, the proposed method showed good precision, acceptable stability and reproducibility, indicating the immunosensor could be used for the sensitive, efficient and real-time detection of cortisol in real samples. Therefore, the present strategy provides a novel and convenient method for clinical determination of cortisol.
authors: School of Pharmacy, Lanzhou University, Lanzhou, 730000 China.
authors: Bolu
authors: B
authors: Sun
authors: Lanzhou Military Command Center for Disease Prevention and Control, Lanzhou, 730000 China.
authors: Yuqiang
authors: Y
authors: Gou
authors: School of Pharmacy, Lanzhou University, Lanzhou, 730000 China.
authors: Yuling
authors: Y
authors: Ma
authors: School of Pharmacy, Lanzhou University, Lanzhou, 730000 China.
authors: Xiaoping
authors: X
authors: Zheng
authors: School of Pharmacy, Lanzhou University, Lanzhou, 730000 China.
authors: Ruibin
authors: R
authors: Bai
authors: School of Pharmacy, Lanzhou University, Lanzhou, 730000 China.
authors: Ahmed Attia
authors: AA
authors: Ahmed Abdelmoaty
authors: School of Pharmacy, Lanzhou University, Lanzhou, 730000 China. Electronic address: hufd@lzu.edu.cn.
authors: Fangdi
authors: F
authors: Hu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008958:Models, Molecular; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D010087:Oxides; D006046:Gold; D006108:Graphite; D006854:Hydrocortisone
keywords: Cortisol (Cor); Electrochemical immunosensor; Gold nanoparticles (AuNPs); Magnetic functionalized reduced graphene oxide (MrGO)