title: Proteome-wide antigen discovery of novel protective vaccine candidates against Staphylococcus aureus infection.
abstract: Methicillin-resistant Staphylococcus aureus (MRSA) is a rapidly growing problem, especially in hospitals where MRSA cause increased morbidity and mortality and a significant rise in health expenditures. As many strains of MRSA are resistant to other antimicrobials in addition to methicillin, there is an urgent need to institute non-antimicrobial measures, such as vaccination, against the spread of MRSA. With the aim of finding new protective antigens for vaccine development, this study used a proteome-wide in silico antigen prediction platform to screen the proteome of S. aureus strain MRSA252. Thirty-five different S. aureus proteins were identified, recombinantly expressed, and tested for protection in a lethal sepsis mouse model using S. aureus strain MRSA252 as the challenge organism. We found that 13 of the 35 recombinant peptides yielded significant protection and that 12 of these antigens were highly conserved across 70 completely sequenced S. aureus strains. Thus, this in silico platform was capable of identifying novel candidates for inclusion in future vaccines against MRSA.
authors: Department of Cancer and Inflammation Research, University of Southern Denmark, Odense, Denmark. Electronic address: krasmussen@health.sdu.dk.
authors: Karina Juhl
authors: KJ
authors: Rasmussen
authors: Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
authors: Andreas Holm
authors: AH
authors: Mattsson
authors: Department of Cancer and Inflammation Research, University of Southern Denmark, Odense, Denmark.
authors: Katrine
authors: K
authors: Pilely
authors: Department of Cancer and Inflammation Research, University of Southern Denmark, Odense, Denmark.
authors: Cecilie Antoinette
authors: CA
authors: Asferg
authors: Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
authors: Oana
authors: O
authors: Ciofu
authors: Department of Cancer and Inflammation Research, University of Southern Denmark, Odense, Denmark.
authors: Lars
authors: L
authors: Vitved
authors: Department of Cancer and Inflammation Research, University of Southern Denmark, Odense, Denmark.
authors: Claus
authors: C
authors: Koch
authors: Department of Clinical Microbiology, University of Southern Denmark, Odense, Denmark.
authors: Michael
authors: M
authors: Kemp
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D017124:Conserved Sequence; D005260:Female; D055624:Methicillin-Resistant Staphylococcus aureus; D051379:Mice; D020543:Proteome; D011994:Recombinant Proteins / Q000276:immunology; D012984:Software; D013203:Staphylococcal Infections / Q000517:prevention & control*; D013209:Staphylococcal Vaccines / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D020543:Proteome; D011994:Recombinant Proteins; D013209:Staphylococcal Vaccines
keywords: Antigen discovery; Proteome analysis; Staphylococcus aureus; Vaccine