title: Brevibacterium massiliense (Roux and Raoult 2009) is a later heterotypic synonym of Brevibacterium ravenspurgense (Mages, Frodl, Bernard and Funke 2009), using whole-genome sequence analysis as a comparative tool.
abstract: A patient strain derived from urine was found by 16S rRNA gene sequencing to be closely related (99.6 % identity) to sequences derived from both Brevibacterium ravenspurgense CCUG 56047T and Brevibacterium massilienseCCUG 53855T. Those species had been described during the same 11 month period in 2008-2009. Further characterization revealed that those isolates could not be readily distinguished from each other biochemically, by cellular fatty acids, antimicrobial susceptibility, MALDI-TOF MS, 16S rRNA gene sequencing or by whole-genome sequence (WGS) analyses. By WGS comparison, these isolates had an aerage nucleotide identity using blastn (ANIb) scores of 95.7 % or higher to each other, DNA G+C content in the range of 62.3 mol%-62.4 mol%, with genome sizes ranging from 2.28×106 to 2.41×106 bases. Based on these data, we propose that the name B. massiliense is a later heterotypic synonym of B. ravenspurgense and provide an emended description of B. ravenspurgense.
authors: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.|National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors: Kathryn A
authors: KA
authors: Bernard
authors: National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors: Ana Luisa
authors: AL
authors: Pacheco
authors: National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors: Tamara
authors: T
authors: Burdz
authors: National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors: Deborah
authors: D
authors: Wiebe
authors: National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors: Chris
authors: C
authors: Huynh
authors: National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors: Christine
authors: C
authors: Bonner
authors: Health PEI, Queen Elizabeth Hospital, Charlottetown, PEI, Canada.
authors: Greg J
authors: GJ
authors: German
authors: Department of Biology, Université de Saint-Boniface, Winnipeg, Manitoba, Canada.
authors: Anne-Marie
authors: AM
authors: Bernier
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D001951:Brevibacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006801:Humans; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014556:Urine / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S