title: Activity of Cysteamine against the Cystic Fibrosis Pathogen Burkholderia cepacia Complex.
abstract: There are no wholly successful chemotherapeutic strategies against Burkholderia cepacia complex (BCC) colonization in cystic fibrosis (CF). We assessed the impact of cysteamine (Lynovex) in combination with standard-of-care CF antibiotics in vitro against BCC CF isolates by the concentration at which 100% of bacteria were killed (MIC100) and checkerboard assays under CLSI standard conditions. Cysteamine facilitated the aminoglycoside-, fluoroquinolone- and folate pathway inhibitor-mediated killing of BCC organisms that were otherwise resistant or intermediately sensitive to these antibiotic classes. Slow-growing BCC strains are often recalcitrant to treatment and form biofilms. In assessing the impact of cysteamine on biofilms, we demonstrated inhibition of BCC biofilm formation at sub-MIC100s of cysteamine.
authors: NovaBiotics Ltd., Craibstone, Bucksburn, Aberdeen, United Kingdom doug@novabiotics.co.uk.
authors: Douglas
authors: 0000-0002-2846-6772
authors: D
authors: Fraser-Pitt
authors: NovaBiotics Ltd., Craibstone, Bucksburn, Aberdeen, United Kingdom.
authors: Derry
authors: D
authors: Mercer
authors: NovaBiotics Ltd., Craibstone, Bucksburn, Aberdeen, United Kingdom.
authors: Emma
authors: E
authors: Lovie
authors: NovaBiotics Ltd., Craibstone, Bucksburn, Aberdeen, United Kingdom.
authors: Jennifer
authors: J
authors: Robertson
authors: NovaBiotics Ltd., Craibstone, Bucksburn, Aberdeen, United Kingdom.
authors: Deborah
authors: D
authors: O'Neil
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D042602:Burkholderia cepacia complex / Q000187:drug effects* / Q000302:isolation & purification; D003543:Cysteamine / Q000494:pharmacology*; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D014031:Tobramycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D003543:Cysteamine; D014031:Tobramycin