title: Molecular characterization of blaESBL-producing Escherichia coli cultured from pig farms in Ireland.
abstract: To characterize ESBL-encoding Escherichia coli cultured from pigs and their plasmids carrying these genes following conjugation into recipient strains.
  
  
  
  Six ESBL-producing E. coli were recovered from faecal samples taken from pigs along with a further isolate from the environment of a farrowing house on three pig farms in Ireland. These isolates were characterized by phylogenetic grouping, MLST and ESBL genotype analyses. Conjugation experiments were carried out in broth mating assays. S1-nuclease PFGE was used to determine the plasmid profiles. Whole-genome sequences of the seven E. coli were determined and subsequently analysed.
  
  
  
  Phylogenetic groups and the corresponding MLST STs identified among the seven tested E. coli isolates included A/ST10, A/ST34, C/ST23 and C/ST1629. All seven isolates carried one or more high-molecular-weight plasmids and demonstrated the ability to transfer their cefotaxime resistance phenotype at high frequencies. Five transmissible plasmid replicon types were detected, including IncK/B (n = 3), IncI1 (n = 2), IncFIA (n = 1), IncFIB (n = 1) and IncN (n = 1). ESBL-encoding genes, including blaCTX-M-14, blaCTX-M-15and blaTEM-20, were identified.
  
  
  
  As the first report from pig sources in Ireland, characterization of these ESBL-encoding isolates and their transmissible plasmids extends our understanding on these resistance markers from porcine E. coli.
authors: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.|UCD-Centre for Food Safety, School of Public Health, Physiotherapy & Sports Science, University College Dublin, Belfield, Dublin 4, Ireland.
authors: Juan
authors: J
authors: Wang
authors: School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors: James F
authors: JF
authors: Gibbons
authors: UCD-Centre for Food Safety, School of Public Health, Physiotherapy & Sports Science, University College Dublin, Belfield, Dublin 4, Ireland.
authors: Kathleen
authors: K
authors: McGrath
authors: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Centre for Food Safety Risk Assessment, 7 Panjiayuan Nanli, Chaoyang District, Beijing 100021, China.
authors: Li
authors: L
authors: Bai
authors: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Centre for Food Safety Risk Assessment, 7 Panjiayuan Nanli, Chaoyang District, Beijing 100021, China.
authors: Fengqin
authors: F
authors: Li
authors: School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors: Finola C
authors: FC
authors: Leonard
authors: Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 272, CH-8057 Zurich, Switzerland.
authors: Roger
authors: R
authors: Stephan
authors: UCD-Centre for Food Safety, School of Public Health, Physiotherapy & Sports Science, University College Dublin, Belfield, Dublin 4, Ireland sfanning@ucd.ie.|Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Centre for Food Safety Risk Assessment, 7 Panjiayuan Nanli, Chaoyang District, Beijing 100021, China.|Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Stranmillis Road, Belfast BT9 5AG, Northern Ireland.
authors: Séamus
authors: S
authors: Fanning
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D003227:Conjugation, Genetic; D004783:Environmental Microbiology; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D000072480:Farms; D005243:Feces / Q000382:microbiology; D014644:Genetic Variation; D007494:Ireland; D058889:Molecular Typing; D010802:Phylogeny; D010957:Plasmids / Q000032:analysis; D017422:Sequence Analysis, DNA; D013552:Swine / Q000382:microbiology*; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases