title: Molecular phylogeny and taxonomy of Lagenidium-like oomycetes pathogenic to mammals.
abstract: Over the past twenty years, infections caused by previously unrecognised oomycete pathogens with morphological and molecular similarities to known Lagenidium species have been observed with increasing frequency, primarily in dogs but also in cats and humans. Three of these pathogens were formally described as Lagenidium giganteum forma caninum, Lagenidium deciduum, and Paralagenidium karlingii in advance of published phylogenetic verification. Due to the complex nature of Lagenidium taxonomy alongside recent reports of mammalian pathogenic species, these taxa needed to be verified with due consideration of the available data for Lagenidium and its allied genera. This study does so through morphologic characterisation of the mammalian pathogenic species, and phylogenetic analyses. The six-gene phylogeny generally supports the most recent comprehensive classification of Lagenidium with a well-supported Lagenidium clade that includes the mammalian pathogens L. giganteum f. caninum and L. deciduum, and well-supported clades for which the names Myzocytiopsis and Salilagenidium can be applied. The genus Paralagenidium is phylogenetically unrelated to any of the main clades within the class Peronosporomycetes. Close relationships between pathogens of mammals and those of insects or nematodes were revealed. Further characterisation of Lagenidium-like taxa is needed to establish the risk of mammalian infection by pathogens of insects and nematodes.
authors: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, K1A 0C6, Ontario, Canada. Electronic address: aspiestog@gmail.com.
authors: Christoffel F J
authors: CFJ
authors: Spies
authors: Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
authors: Amy M
authors: AM
authors: Grooters
authors: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, K1A 0C6, Ontario, Canada.
authors: C André
authors: CA
authors: Lévesque
authors: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, K1A 0C6, Ontario, Canada.
authors: Tara L
authors: TL
authors: Rintoul
authors: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, K1A 0C6, Ontario, Canada.
authors: Scott A
authors: SA
authors: Redhead
authors: 135 Brodrick Road, Eastbourne, BN22 9RA, UK.
authors: Sally L
authors: SL
authors: Glockling
authors: Department of Plant Pathology, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan.
authors: Chi-Yu
authors: CY
authors: Chen
authors: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors: Arthur W A M
authors: AWAM
authors: de Cock
mesh_terms: D000818:Animals; D002415:Cats; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004285:Dogs; D005656:Fungal Proteins / Q000235:genetics; D020459:Genes, rRNA; D006801:Humans; D044726:Lagenidium / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D008853:Microscopy; D024101:Mitochondrial Proteins / Q000235:genetics; D009181:Mycoses / Q000382:microbiology* / Q000662:veterinary*; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D024101:Mitochondrial Proteins; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S
keywords: Chlamydomyzium; Myzocytiopsis; Oomycetes; Paralagenidium; Phylogeny; Salilagenidium