title: Comparative Transcriptome Profiling of Human Foreskin Fibroblasts Infected with the Sylvio and Y Strains of Trypanosoma cruzi.
abstract: Trypanosoma cruzi, the causative agent of Chagas Disease, is phylogeneticaly distributed into nearly identical genetic strains which show divergent clinical presentations including differences in rates of cardiomyopathy in humans, different vector species and transmission cycles, differential congenital transmission in a mouse model, and differing immune and heart inflammation response in dogs. The population structure of these strains divides into two groups, which are geographically and clinically distinct. The aim of this study was to compare the transcriptome of two strains of T. cruzi, Sylvio vs. Y, to identify differences in expression that could account for clinical and biochemical differences. We collected and sequenced RNA from T. cruzi-infected and control Human Foreskin Fibroblasts at three timepoints. Differential expression analysis identified gene expression different timepoints in Sylvio infections, and between Sylvio and Y infections in both parasite and host. The Sylvio strain parasite and the host response to Sylvio infection largely mirrored the host-pathogen interaction seen in our previous Y strain work. IL-8 was more highly expressed in Sylvio-infected HFFs than in Y-infected HFFs.
authors: Department of Cell Biology and Molecular Genetics, College of Computational, Mathematical and Natural Science, University of Maryland, College Park, Maryland, United States of America.|Center for Bioinformatics and Computational Biology, University of Maryland Institute for Advanced Computer Studies (UMIACS), University of Maryland, College Park, Maryland, United States of America.
authors: Genevieve A
authors: GA
authors: Houston-Ludlam
authors: Department of Cell Biology and Molecular Genetics, College of Computational, Mathematical and Natural Science, University of Maryland, College Park, Maryland, United States of America.|Center for Bioinformatics and Computational Biology, University of Maryland Institute for Advanced Computer Studies (UMIACS), University of Maryland, College Park, Maryland, United States of America.
authors: A Trey
authors: AT
authors: Belew
authors: Department of Cell Biology and Molecular Genetics, College of Computational, Mathematical and Natural Science, University of Maryland, College Park, Maryland, United States of America.|Center for Bioinformatics and Computational Biology, University of Maryland Institute for Advanced Computer Studies (UMIACS), University of Maryland, College Park, Maryland, United States of America.
authors: Najib M
authors: NM
authors: El-Sayed
mesh_terms: D005347:Fibroblasts / Q000378:metabolism* / Q000469:parasitology*; D052816:Foreskin / Q000166:cytology*; D020869:Gene Expression Profiling; D006790:Host-Parasite Interactions; D006801:Humans; D008297:Male; D013045:Species Specificity; D014349:Trypanosoma cruzi / Q000502:physiology*