title: CMV70-3P miRNA contributes to the CMV mediated glioma stemness and represents a target for glioma experimental therapy.
abstract: Glioblastoma multiforme (GBM) is a rapidly progressive brain tumor with a median survival of 15-19 months. Therapeutic resistance and recurrence of the disease is attributed to cancer stem cells (CSC). Here, we report that CMV70-3P miRNA encoded by CMV increases GBM CSC stemness. Inhibition of CMV70-3P expression using oligo inhibitors significantly attenuated the ability of primary glioma cells to proliferate and form neurospheres. At the molecular level, we show that CM70-3P increases expression of cellular SOX2. Collectively, these findings indicate that CMV70-3P is a potential regulator of CMV- mediated glioma progression and cancer stemness.
authors: Swedish Neuroscience Institute, Center for Advanced Brain Tumor Treatment, Seattle, WA, 98122, USA.|Institute of Experimental Diagnostics and Therapy of Tumors, N.N. Blokhin Russian Cancer Research Center, Moscow, 115478, Russia.|NN. Blokhin Cancer Research Center, RAMN, Moscow, 115478, Russia.
authors: Ilya V
authors: IV
authors: Ulasov
authors: NN. Blokhin Cancer Research Center, RAMN, Moscow, 115478, Russia.|Current employment: Division of Nephrology, University of Washington, Seattle, 98109, WA, USA.
authors: Natalya V
authors: NV
authors: Kaverina
authors: Swedish Neuroscience Institute, Center for Advanced Brain Tumor Treatment, Seattle, WA, 98122, USA.
authors: Dhimankrishna
authors: D
authors: Ghosh
authors: Institute of Experimental Diagnostics and Therapy of Tumors, N.N. Blokhin Russian Cancer Research Center, Moscow, 115478, Russia.|NN. Blokhin Cancer Research Center, RAMN, Moscow, 115478, Russia.
authors: Marya A
authors: MA
authors: Baryshnikova
authors: NN. Blokhin Cancer Research Center, RAMN, Moscow, 115478, Russia.
authors: Zaira G
authors: ZG
authors: Kadagidze
authors: NN. Blokhin Cancer Research Center, RAMN, Moscow, 115478, Russia.
authors: Apollon I
authors: AI
authors: Karseladze
authors: Institute of Experimental Diagnostics and Therapy of Tumors, N.N. Blokhin Russian Cancer Research Center, Moscow, 115478, Russia.|NN. Blokhin Cancer Research Center, RAMN, Moscow, 115478, Russia.
authors: Anatoly Y
authors: AY
authors: Baryshnikov
authors: Swedish Neuroscience Institute, Center for Advanced Brain Tumor Treatment, Seattle, WA, 98122, USA.
authors: Charles S
authors: CS
authors: Cobbs
mesh_terms: D000071916:AC133 Antigen / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000821:virology*; D015870:Gene Expression; D005910:Glioma / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D035683:MicroRNAs; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012367:RNA, Viral; D055748:SOXB1 Transcription Factors / Q000235:genetics
chemical_list: D000071916:AC133 Antigen; D035683:MicroRNAs; D012367:RNA, Viral; D055748:SOXB1 Transcription Factors
keywords: brain tumor; cytomegalovirus; glioma stem cells; miRNA