title: Effect of electrolytic lesions of the dorsal diencephalic conduction system on the distribution of Fos-like immunoreactivity induced by rewarding electrical stimulation.
abstract: The dorsal diencephalic conduction system (DDC) is an important pathway of the brain reward circuitry, linking together forebrain and midbrain structures. The present work was aimed at describing the effect of a DDC lesion on the distribution of Fos-like immunoreactivity (FLIR) following intracranial self-stimulation (ICSS) of the lateral hypothalamus (LH). Rats were implanted with monopolar electrodes and divided into three groups; the first two groups were trained to self-stimulate at the LH, whereas the third group received no stimulation and served as a control. Among the two groups that were trained for ICSS, one of them received a lesion at the DDC and was tested for ICSS on the subsequent 5days. On the last day of testing, control rats were placed in operant chambers without receiving any stimulation, and the remaining rats were allowed to receive the stimulation for 1h. All rats were then processed for FLIR. As previously shown, a lesion at the DDC resulted in significant attenuations of the rewarding effectiveness of LH stimulation. Results also show a higher FLIR in several reward-related areas following LH stimulation, especially in the hemisphere ipsilateral to the stimulation electrode. Compared to non-lesioned rats, lesioned animals had lower FLIR in certain brain regions, suggesting that those regions that were activated by the rewarding stimulation may be functionally interconnected with the DDC.
authors: Department of Neurosciences, Faculty of Medicine, Université de Montréal, Montreal, Quebec, Canada.
authors: Marc
authors: M
authors: Fakhoury
authors: Faculty of Pharmacy and FRQ-S Research Group on Central Nervous System, Université de Montréal, Montreal, Quebec, Canada.
authors: David
authors: D
authors: Voyer
authors: Faculty of Pharmacy and FRQ-S Research Group on Central Nervous System, Université de Montréal, Montreal, Quebec, Canada.
authors: Daniel
authors: D
authors: Lévesque
authors: Department of Neurosciences, Faculty of Medicine, Université de Montréal, Montreal, Quebec, Canada; FRQ-S Research Group in Behavioral Neurobiology, Concordia University, Montreal, Quebec, Canada. Electronic address: pierre-paul.rompre@umontreal.ca.
authors: Pierre-Paul
authors: PP
authors: Rompré
mesh_terms: D000818:Animals; D003216:Conditioning, Operant / Q000502:physiology; D004027:Diencephalon / Q000473:pathology / Q000502:physiology* / Q000503:physiopathology; D004558:Electric Stimulation; D007839:Functional Laterality; D007150:Immunohistochemistry; D058542:Implantable Neurostimulators; D008297:Male; D008636:Mesencephalon / Q000473:pathology / Q000502:physiology* / Q000503:physiopathology; D009434:Neural Pathways / Q000473:pathology / Q000502:physiology / Q000503:physiopathology; D016548:Prosencephalon / Q000473:pathology / Q000502:physiology* / Q000503:physiopathology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D020318:Rats, Long-Evans; D012201:Reward; D012653:Self Stimulation
chemical_list: D016760:Proto-Oncogene Proteins c-fos
keywords: c-Fos; dorsal diencephalic conduction system; lateral habenula; lateral hypothalamus