title: Association of IL-1β +3953 C and HLA-DRB1*15 with Coronary Artery and Rheumatic Heart Diseases in South India.
abstract: Among the various candidate genes predisposing for cardiovascular diseases, HLA-DRB1* and IL-1β +3953C/T alleles have been implicated repeatedly. To test these in South India, we carried out a case control study of 323 Coronary Artery Disease (CAD) patients, 56 Rheumatic Heart Disease (RHD) patients and 254 endemic controls. The polymorphisms were studied by PCR - SSP and ARMS-PCR methods and results analyzed for various clinical and demographic parameters. In CAD, HLA-DRB1*14 allele showed significant predisposition (OR: 2.19; 95% CI: 1.04-4.58; p value=0.023), particularly in male patients (OR: 4.07; 95% CI: 1.20-13.81; p value=0.01) and further in males with Triple Vessel Disease (OR: 5.49; 95% CI: 1.45-20.60; p value=0.007). On the other hand, HLA-DRB1*15 predisposed for RHD (OR: 3.56; 95% CI: 1.87-6.78; p value=0.001) in both the genders. Population stratification showed this higher risk association in Vanniyar caste (OR: 5.00; 95% CI: 1.27-19.59; p value=0.022). Among the IL1-β +3953C/T polymorphism, the ancestral allele 'C' showed a significant high risk association with CAD (OR: 1.83; 95% CI: 1.24-2.70; p value=0.001), particularly in Mudaliar (OR: 6.07; 95% CI: 1.77-20.74; p value=0.003; AF=0.7) and Vanniyar castes (OR: 3.67; 95% CI: 0.92-14.57; p value=0.05; AF=0.660). Two different cardiac ailments studied, RHD & CAD thus showed varied associations in this South Indian cohorts. RHD having an infectious aetiology shared a HLA-DRB1*15 high risk association, while HLA-DRB1*14 and IL-1β +3953C predisposed for CAD, an inflammatory disorder, reiterating the diverse genetic predisposition of the two cardiac ailments studied.
authors: Department of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Chennai 603103, India.
authors: M S
authors: MS
authors: Sreekanth
authors: Department of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Chennai 603103, India.
authors: S K
authors: SK
authors: Esdan Basha
authors: School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur 613401, India.
authors: G
authors: G
authors: Arun Kumar
authors: Department of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Chennai 603103, India.
authors: S
authors: S
authors: Govindaraju
authors: Department of Cardiology, Chettinad Hospital and Research Institute, Kelambakkam, Chennai 603103, India.
authors: N
authors: N
authors: Pradeep Nayar
authors: Department of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Chennai 603103, India. Electronic address: pitchappanrm@yahoo.co.uk.
authors: R M
authors: RM
authors: Pitchappan
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000235:genetics*; D005006:Ethnicity; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006801:Humans; D007194:India; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012214:Rheumatic Heart Disease / Q000235:genetics*
chemical_list: D059811:HLA-DRB1 Chains; C507799:HLA-DRB1*15 antigen; D053583:Interleukin-1beta
keywords: CAD; HLA-DRB; IL-1β; RHD