title: Temporal control of xyloglucan self-assembly into layered structures by radiation-induced degradation.
abstract: Partially degalactosylated xyloglucan from tamarind seeds (Deg-XG) is a very appealing biopolymer for the production of in situ gelling systems at physiological temperature. In this work, we observe that the morphology of hydrogels evolves towards high degrees of structural organization with time, yielding to dense stacks of thin membranes within 24h of incubation at 37°C. We also explore the possibility offered by gamma irradiation of controlling the time scale of this phenomenon, the final morphology and mechanical properties of the system. Structural and molecular modifications of Deg-XG with dose are investigated by FTIR, dynamic light scattering (DLS) and rotational viscosimetry. The impact on gelation ability and gel strength is studied by rheological analysis. The morphology evolution is investigated by SEM analysis, and absence of cytotoxicity verified by MTS assay and optical microscopy of neuroblastoma cells.
authors: Dipartimento di Ingegneria Chimica, Gestionale, Informatica, Meccanica, Università degli Studi di Palermo, Viale delle Scienze Ed. 6, 90128 Palermo, Italy.
authors: Simona
authors: S
authors: Todaro
authors: Dipartimento di Ingegneria Chimica, Gestionale, Informatica, Meccanica, Università degli Studi di Palermo, Viale delle Scienze Ed. 6, 90128 Palermo, Italy.
authors: Maria Antonietta
authors: MA
authors: Sabatino
authors: CNR - Istituto di Biofisica (Palermo Unit), Via U. La Malfa 153, 90146 Palermo, Italy.
authors: Maria Rosalia
authors: MR
authors: Mangione
authors: CNR - Istituto di Biomedicina e Immunologia Molecolare "A. Monroy" (IBIM), Via U. La Malfa, 153, 90146 Palermo, PA, Italy.
authors: Pasquale
authors: P
authors: Picone
authors: CNR - Istituto di Biomedicina e Immunologia Molecolare "A. Monroy" (IBIM), Via U. La Malfa, 153, 90146 Palermo, PA, Italy.
authors: Maria Laura
authors: ML
authors: Di Giacinto
authors: CNR - Istituto di Biofisica (Palermo Unit), Via U. La Malfa 153, 90146 Palermo, Italy.
authors: Donatella
authors: D
authors: Bulone
authors: Dipartimento di Ingegneria Chimica, Gestionale, Informatica, Meccanica, Università degli Studi di Palermo, Viale delle Scienze Ed. 6, 90128 Palermo, Italy. Electronic address: clelia.dispenza@unipa.it.
authors: Clelia
authors: C
authors: Dispenza
mesh_terms: D001704:Biopolymers / Q000737:chemistry / Q000493:pharmacokinetics / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005720:Gamma Rays; D005936:Glucans / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000528:radiation effects; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics*; D008422:Materials Testing; D009447:Neuroblastoma / Q000473:pathology; D058105:Polymerization / Q000528:radiation effects; D033081:Shear Strength; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D014783:Viscosity; D014990:Xylans / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000528:radiation effects
chemical_list: D001704:Biopolymers; D005936:Glucans; D020100:Hydrogels; D014990:Xylans; C029353:xyloglucan
keywords: High energy-irradiation; Hydrogels; Self-assembly; Thermoresponsive biopolymer; Xyloglucan