title: Morphological and molecular identification of nasopharyngeal bot fly larvae infesting red deer (Cervus elaphus) in Austria.
abstract: Nasopharyngeal myiases are caused by larvae of bot flies (Diptera: Oestridae), which have evolved a high specificity for their hosts. Bot flies (n = 916) were collected from 137 (57.6 %) out of 238 red deer (Cervus elaphus) hunted in Vorarlberg and Tyrol (Western Austria). After being stored in 75 % ethanol, larvae were identified to species level and developmental stage using morphological and morphometric keys. Larvae were also molecularly characterized by polymerase chain reaction (PCR) amplification and partial sequencing of the mitochondrial cytochrome oxidase subunit I gene. Morphological and molecular analysis allowed identification of larvae as Cephenemyia auribarbis and Pharyngomyia picta. Genetic variations were also examined within the specimens collected in both geographical locations.
authors: Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210, Vienna, Austria.
authors: Natascha
authors: N
authors: Leitner
authors: Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210, Vienna, Austria.
authors: Laurin
authors: L
authors: Schwarzmann
authors: Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210, Vienna, Austria.
authors: Carina
authors: C
authors: Zittra
authors: Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210, Vienna, Austria.
authors: Nicola
authors: N
authors: Palmieri
authors: Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210, Vienna, Austria.
authors: Barbara
authors: B
authors: Eigner
authors: Department of Veterinary Medicine, University of Bari, Str. Prov. Casamassima km3, 70010, Valenzano, Bari, Italy.
authors: Domenico
authors: D
authors: Otranto
authors: Institute for Veterinary Disease Control Innsbruck, Austrian Agency for Health and Food Safety Ltd. (AGES), Technikerstrasse 70, 6020, Innsbruck, Austria.
authors: Walter
authors: W
authors: Glawischnig
authors: Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210, Vienna, Austria. hans-peter.fuehrer@vetmeduni.ac.at.
authors: Hans-Peter
authors: http://orcid.org/0000-0002-4178-0133
authors: HP
authors: Fuehrer
mesh_terms: D000818:Animals; D001317:Austria; D003670:Deer / Q000469:parasitology*; D004175:Diptera / Q000145:classification; D007814:Larva; D058889:Molecular Typing; D009198:Myiasis / Q000469:parasitology / Q000662:veterinary*; D009302:Nasopharyngeal Diseases / Q000469:parasitology / Q000662:veterinary*; D009305:Nasopharynx / Q000469:parasitology
keywords: Cephenemyia auribarbis; Cervus elaphus; Cytochrome oxidase subunit I; Myiasis; Oestridae; Pharyngomyia picta