title: Enhanced exercise and regenerative capacity in a mouse model that violates size constraints of oxidative muscle fibres.
abstract: A central tenet of skeletal muscle biology is the existence of an inverse relationship between the oxidative fibre capacity and its size. However, robustness of this relationship is unknown. We show that superimposition of Estrogen-related receptor gamma (Errγ) on the myostatin (Mtn) mouse null background (Mtn(-/-)/Errγ(Tg/+)) results in hypertrophic muscle with a high oxidative capacity thus violating the inverse relationship between fibre size and oxidative capacity. We also examined the canonical view that oxidative muscle phenotype positively correlate with Satellite cell number, the resident stem cells of skeletal muscle. Surprisingly, hypertrophic fibres from Mtn(-/-)/Errγ(Tg/+) mouse showed satellite cell deficit which unexpectedly did not affect muscle regeneration. These observations 1) challenge the concept of a constraint between fibre size and oxidative capacity and 2) indicate the important role of the microcirculation in the regenerative capacity of a muscle even when satellite cell numbers are reduced.
authors: School of Biological Sciences, University of Reading, Reading, United Kingdom.
authors: Saleh
authors: S
authors: Omairi
authors: Hull York Medical School, Hull, United Kingdom.
authors: Antonios
authors: A
authors: Matsakas
authors: School of Healthcare Science, Manchester Metropolitan University, Manchester, United Kingdom.|Lithuanian Sports University, Kaunas, Lithuania.
authors: Hans
authors: H
authors: Degens
authors: Renal Division, University Medical Center Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.
authors: Oliver
authors: O
authors: Kretz
authors: Centre for Integrative Neuroplasticity, Department of Biosciences, University of Oslo, Oslo, Norway.
authors: Kenth-Arne
authors: KA
authors: Hansson
authors: Centre for Integrative Neuroplasticity, Department of Biosciences, University of Oslo, Oslo, Norway.|Department of Physics, University of Oslo, Oslo, Norway.
authors: Andreas Våvang
authors: AV
authors: Solbrå
authors: Centre for Integrative Neuroplasticity, Department of Biosciences, University of Oslo, Oslo, Norway.|Department of Health Sciences, Kristiania University College, Oslo, Norway.
authors: Jo C
authors: JC
authors: Bruusgaard
authors: Department of Neuroanatomy, University of Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.
authors: Barbara
authors: B
authors: Joch
authors: Venetian Institute of Molecular Medicine, University of Padua, Padua, Italy.
authors: Roberta
authors: R
authors: Sartori
authors: Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom.
authors: Natasa
authors: N
authors: Giallourou
authors: School of Biological Sciences, University of Reading, Reading, United Kingdom.
authors: Robert
authors: R
authors: Mitchell
authors: School of Biological Sciences, University of Reading, Reading, United Kingdom.
authors: Henry
authors: H
authors: Collins-Hooper
authors: School of Biological Sciences, University of Reading, Reading, United Kingdom.
authors: Keith
authors: K
authors: Foster
authors: Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland.
authors: Arja
authors: A
authors: Pasternack
authors: Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland.
authors: Olli
authors: O
authors: Ritvos
authors: Venetian Institute of Molecular Medicine, University of Padua, Padua, Italy.
authors: Marco
authors: M
authors: Sandri
authors: Institute of Molecular Medicine, University of Health Science Center, Houston, Texas.
authors: Vihang
authors: V
authors: Narkar
authors: Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom.
authors: Jonathan R
authors: JR
authors: Swann
authors: Renal Division, University Medical Center Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.|BIOSS Center for Biological Signalling Studies, Albert-Ludwigs-University Freiburg, Houston, Texas.|FRIAS, Freiburg Institute for Advanced Studies and Center for Biological System Analysis ZBSA, Freiburg, Germany.
authors: Tobias B
authors: TB
authors: Huber
authors: School of Biological Sciences, University of Reading, Reading, United Kingdom.|FRIAS, Freiburg Institute for Advanced Studies and Center for Biological System Analysis ZBSA, Freiburg, Germany.
authors: Ketan
authors: 0000-0002-7131-749X
authors: K
authors: Patel
mesh_terms: D000818:Animals; D051379:Mice; D018345:Mice, Knockout; D018485:Muscle Fibers, Skeletal / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology* / Q000502:physiology*; D055435:Myostatin / Q000172:deficiency; D010805:Physical Conditioning, Animal; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000502:physiology*
chemical_list: C525912:Mstn protein, mouse; D055435:Myostatin
keywords: developmental biology; metabolism; mouse; muscle; regeneration; stem cell; stem cells