title: Immunolabeling and In Situ Labeling of Isolated Plant Interphase Nuclei.
abstract: Specific labeling of proteins and nucleic acids by immunofluorescence or in situ techniques is an important adjunct to microscopical analysis for cell biology. Labeling of nuclear structures in intact complex tissues is often hampered by problems of penetration of the macromolecular labeling reagents needed. Here we describe a method of labeling isolated plant nuclei that we have found to be a useful approach that can help to overcome these problems.
authors: Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors: Ali
authors: A
authors: Pendle
authors: Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK. peter.shaw@jic.ac.uk.
authors: Peter
authors: P
authors: Shaw
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics*; D002467:Cell Nucleus / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique / Q000379:methods*; D007124:Immunoenzyme Techniques / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D007399:Interphase
chemical_list: D018744:DNA, Plant
keywords: Fluorescence microscopy; Immunolabeling; In situ hybridization; Nuclei; Nuclei isolation; Plant cell biology