title: Real-time optical diagnosis of gastric cancer with serosal invasion using multiphoton imaging.
abstract: A real-time optical biopsy, which could determine tissue histopathology, would be of extraordinary benefit to staging laparoscopy for gastric cancer with serosal invasion (T4) that requires downstage treatment. We investigated the feasibility of using multiphoton imaging to perform a real-time optical diagnosis of gastric cancer with or without serosal invasion. First, a pilot study was performed to establish the optical diagnostic features of gastric cancer with or without serosal invasion using multiphoton imaging compared with hematoxylin-eosin staining and Masson's trichrome staining. Second, a blinded study was performed to compare the diagnostic sensitivity, specificity, and accuracy of multiphoton imaging and endoscopic ultrasonography (EUS) for T4 gastric cancer. In the pilot study, multiphoton imaging revealed collagen loss and degradation and cellular and nuclear pleomorphism in gastric cancer with serosal invasion. The collagen content in gastric cancer with or without serosal invasion was 0.36 ± 0.18 and 0.79 ± 0.16 (p < 0.001), respectively. In the blinded study, the sensitivity, specificity, and accuracy of EUS and multiphoton imaging for T4 gastric cancer were 70% and 90% (p = 0.029), 66.67% and 96.67% (p = 0.003), and 68.33% and 93.33% (p = 0.001), respectively. It is feasible to use multiphoton imaging to make a real-time optical diagnosis of gastric cancer with or without serosal invasion.
authors: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.|Fujian Provincial Key Laboratory for Photonics Technology &Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, P. R. China.
authors: Jun
authors: J
authors: Yan
authors: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.|Department of Surgery, Fujian Provincial Hospital, Teaching Hospital of Fujian Medical University, Fuzhou, Fujian, 350001, P. R. China.
authors: Yu
authors: Y
authors: Zheng
authors: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.|Department of Surgery, Fujian Provincial Hospital, Teaching Hospital of Fujian Medical University, Fuzhou, Fujian, 350001, P. R. China.
authors: Xiaoling
authors: X
authors: Zheng
authors: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
authors: Zhangyuanzhu
authors: Z
authors: Liu
authors: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.|Fujian Provincial Key Laboratory for Photonics Technology &Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, P. R. China.
authors: Wenju
authors: W
authors: Liu
authors: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
authors: Dexin
authors: D
authors: Chen
authors: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
authors: Xiaoyu
authors: X
authors: Dong
authors: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
authors: Kai
authors: K
authors: Li
authors: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
authors: Xiumin
authors: X
authors: Liu
authors: Department of Pathology, Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou, Fujian, 350014, P. R. China.
authors: Gang
authors: G
authors: Chen
authors: Department of Pathology, Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou, Fujian, 350014, P. R. China.|Department of Surgery, Fujian Provincial Tumor Hospital, TeachingHospital of Fujian Medical University, Fuzhou, Fujian, 350014, P. R. China.
authors: Jianping
authors: J
authors: Lu
authors: Fujian Provincial Key Laboratory for Photonics Technology &Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, P. R. China.
authors: Jianxin
authors: J
authors: Chen
authors: Fujian Provincial Key Laboratory for Photonics Technology &Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, P. R. China.
authors: Shuangmu
authors: S
authors: Zhuo
authors: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
authors: Guoxin
authors: G
authors: Li
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005743:Gastrectomy; D006801:Humans; D021621:Imaging, Three-Dimensional; D008297:Male; D036641:Microscopy, Fluorescence, Multiphoton; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D010865:Pilot Projects; D012680:Sensitivity and Specificity; D012704:Serous Membrane / Q000473:pathology; D013274:Stomach Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology / Q000601:surgery