title: In vitro toxicity of cationic micelles and liposomes in cultured human hepatocyte (HepG2) and lung epithelial (A549) cell lines.
abstract: The aim of this study was to compare the effects of cationic micelle and liposome drug delivery systems on liver and lung cells in a toxicological in vitro screening model, with observations on cytotoxicity and genotoxicity. A screening battery was established for assessment of a broad range of parameters related to adverse effects. Clear concentration response effects were observed related to impairment of mitochondrial function, membrane integrity and oxidative stress markers, but no effect was observed on genotoxicity. The adverse effects were highest for the liposomes. The High Content Screening seems optimal for initial screening of adverse effects, and combined with standard cytotoxicity measurements initial screening can be performed for predictive toxicological screening.
authors: Faculty of Health and Medical Science, Department of Public Health, Section of Environmental Health, University of Copenhagen, Denmark. Electronic address: mwro@sund.ku.dk.
authors: Martin
authors: M
authors: Roursgaard
authors: Faculty of Health and Medical Science, Department of Public Health, Section of Environmental Health, University of Copenhagen, Denmark; National Research Center for the Working Environment, Copenhagen, Denmark; H. Lundbeck A/S, Valby, Denmark.
authors: Kristina Bram
authors: KB
authors: Knudsen
authors: H. Lundbeck A/S, Valby, Denmark.
authors: Helle
authors: H
authors: Northeved
authors: H. Lundbeck A/S, Valby, Denmark.
authors: Mikael
authors: M
authors: Persson
authors: H. Lundbeck A/S, Valby, Denmark.
authors: Tina
authors: T
authors: Christensen
authors: Center for Nanomedicine and Theranostics, Technical University of Denmark, DTU Nanotech, Lyngby, Denmark.
authors: Pramod E K
authors: PEK
authors: Kumar
authors: DTU Food, Technical University of Denmark, Søborg, Denmark.
authors: Anders
authors: A
authors: Permin
authors: Center for Nanomedicine and Theranostics, Technical University of Denmark, DTU Nanotech, Lyngby, Denmark.
authors: Thomas L
authors: TL
authors: Andresen
authors: Center for Nanomedicine and Theranostics, Technical University of Denmark, DTU Nanotech, Lyngby, Denmark.
authors: Torben
authors: T
authors: Gjetting
authors: Faculty of Health and Medical Science, Department of Public Health, Section of Environmental Health, University of Copenhagen, Denmark.
authors: Jens
authors: J
authors: Lykkesfeldt
authors: Faculty of Health and Medical Science, Department of Public Health, Section of Environmental Health, University of Copenhagen, Denmark.
authors: Lise K
authors: LK
authors: Vesterdal
authors: Faculty of Health and Medical Science, Department of Public Health, Section of Environmental Health, University of Copenhagen, Denmark.
authors: Steffen
authors: S
authors: Loft
authors: Faculty of Health and Medical Science, Department of Public Health, Section of Environmental Health, University of Copenhagen, Denmark.
authors: Peter
authors: P
authors: Møller
mesh_terms: D000072283:A549 Cells; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000633:toxicity; D016503:Drug Delivery Systems; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000633:toxicity; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008081:Liposomes / Q000633:toxicity*; D008168:Lung / Q000166:cytology; D008823:Micelles; D011092:Polyethylene Glycols / Q000633:toxicity; D011109:Polymethacrylic Acids / Q000633:toxicity; D000644:Quaternary Ammonium Compounds / Q000633:toxicity; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D005229:Fatty Acids, Monounsaturated; C508600:IL6 protein, human; D015850:Interleukin-6; D008081:Liposomes; D008823:Micelles; D011109:Polymethacrylic Acids; D000644:Quaternary Ammonium Compounds; D014409:Tumor Necrosis Factor-alpha; D011092:Polyethylene Glycols; D002784:Cholesterol; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane
keywords: Comet assay; Drug delivery systems; Hepatocytes; In vitro toxicology; Liposomes; Lung epithelial cells; Micelles; Nanomedicine