title: Transcriptomic and Proteomic Research To Explore Bruchid-Resistant Genes in Mungbean Isogenic Lines.
abstract: Mungbean (Vigna radiata (L.) Wilczek) is an important rotation legume crop for human nutrition in Asia. Bruchids (Callosobruchus spp.) currently cause heavy damage as pests of grain legumes during storage. We used omics-related technologies to study the mechanisms of bruchid resistance in seeds of the nearly isogenic lines VC1973A (bruchid-susceptible) and VC6089A (bruchid-resistant). A total of 399 differentially expressed genes (DEGs) were identified between the two lines by transcriptome sequencing. Among these DEGs, 251 exhibited high expression levels and 148 expressed low expression levels in seeds of VC6089A. Forty-five differential proteins (DPs) were identified by isobaric tags for relative and absolute quantification (iTRAQ); 21 DPs had higher abundances in VC6089A, and 24 DPs had higher abundances in VC1973A. According to transcriptome and proteome data, only three DEGs/DPs, including resistant-specific protein (g39185), gag/pol polyprotein (g34458), and aspartic proteinase (g5551), were identified and located on chromosomes 5, 1, and 7, respectively. Both g39185 and g34458 genes encode a protein containing a BURP domain. In previous research on bruchid molecular markers, the g39185 gene located close to the molecular markers of major bruchid-resistant locus may be a bruchid-resistant gene.
authors: Department of Horticulture and Landscape Architecture, National Taiwan University , Taipei 10617, Taiwan.|Institute of Plant and Microbial Biology, Academia Sinica , Taipei 11529, Taiwan.
authors: Wu-Jui
authors: WJ
authors: Lin
authors: Institute of Plant and Microbial Biology, Academia Sinica , Taipei 11529, Taiwan.
authors: Chia-Yun
authors: CY
authors: Ko
authors: Institute of Plant and Microbial Biology, Academia Sinica , Taipei 11529, Taiwan.
authors: Mao-Sen
authors: MS
authors: Liu
authors: Department of Bio-industrial Mechatronics Engineering, National Taiwan University , Taipei 10617, Taiwan.
authors: Chien-Yen
authors: CY
authors: Kuo
authors: Department of Bio-industrial Mechatronics Engineering, National Taiwan University , Taipei 10617, Taiwan.
authors: Dung-Chi
authors: DC
authors: Wu
authors: Department of Bio-industrial Mechatronics Engineering, National Taiwan University , Taipei 10617, Taiwan.
authors: Chien-Yu
authors: CY
authors: Chen
authors: AVRDC-World Vegetable Center , Shanhua, Tainan 74151, Taiwan.
authors: Roland
authors: R
authors: Schafleitner
authors: Institute of Plant and Microbial Biology, Academia Sinica , Taipei 11529, Taiwan.
authors: Long-Fang O
authors: LF
authors: Chen
authors: Department of Horticulture and Landscape Architecture, National Taiwan University , Taipei 10617, Taiwan.
authors: Hsiao-Feng
authors: HF
authors: Lo
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000502:physiology*; D060467:Disease Resistance; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D040901:Proteomics; D059467:Transcriptome; D000070658:Vigna / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
chemical_list: D010940:Plant Proteins
keywords: bruchid; iTRAQ; mungbean; proteome; transcriptome