title: Cerium oxide nanoparticles stimulate proliferation of primary mouse embryonic fibroblasts in vitro.
abstract: The increasing application of cell therapy technologies in the treatment of various diseases requires the development of new effective methods for culturing primary cells. The major limitation for the efficient use of autologous cell material is the low rate of cell proliferation. Successful cell therapy requires sufficient amounts of cell material over a short period of time with the preservation of their differentiation and proliferative potential. In this regard, the development of novel, highly efficient stimulators of proliferative activity in stem cells is a truly urgent task. In this paper we have demonstrated that citrate-stabilized cerium oxide nanoparticles (nanoceria) enhance the proliferative activity of primary mouse embryonic fibroblasts in vitro. Cerium oxide nanoparticles stimulate cell proliferation in a wide range of concentrations (10(-3)М-10(-9)M) through reduction of intracellular levels of reactive oxygen species (ROS) during the lag phase of cell growth and by modulating the expression level of the major antioxidant enzymes. We found the optimal concentration of nanoceria, which provides the greatest acceleration of cell proliferation in vitro, while maintaining the levels of intracellular ROS and mRNA of antioxidant enzymes in the physiological range. Our results confirm that nanocrystalline ceria can be considered as a basis for effective and inexpensive supplements in cell culturing.
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia. Electronic address: antonpopovleonid@gmail.com.
authors: Anton L
authors: AL
authors: Popov
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia.
authors: Nelly R
authors: NR
authors: Popova
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia; Pushchino State Institute of Natural sciences, Pushchino, Moscow region, Russia.
authors: Irina I
authors: II
authors: Selezneva
authors: Kabardino-Balkarian State University, Nalchik, Russia.
authors: Azamat Y
authors: AY
authors: Akkizov
authors: Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; National Research Tomsk State University, Tomsk, Russia.
authors: Vladimir K
authors: VK
authors: Ivanov
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002563:Cerium / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D002563:Cerium; C030583:ceric oxide
keywords: Antioxidant; Cell proliferation; Cerium oxide nanoparticles; Primary embryonic cell; Redox-status