title: Clostridium difficile Adhesins.
abstract: Clostridium difficile is responsible for a large spectrum of intestinal diseases ranging from mild diarrhea to fatal colitis depending on the one hand on the strain virulence and on the other on the host. The pathogenesis of C. difficile infection could be seen as a three-step process that takes place after disruption of the digestive microbiota by antibiotics: (1) contamination by and germination of spores; (2) multiplication of vegetative cells in the colonic niche using colonization factors; (3) production of the two toxins TcdA and TcdB and for some strains an additional toxin, the binary toxin CDT. Several studies have been performed to characterize the bacterial factors involved in the colonization step and particularly adhesins.Here, we describe first the methods used to study C. difficile adherence in vitro to epithelial cells and in vivo in animal model intestinal tract, and second the methods used to demonstrate the adhesive properties of surface proteins using Cwp66, GroEL, and FbpA as examples.
authors: Unité Bactéries Pathogènes et Santé (UBaPS), Faculté de Pharmacie, Université Paris-Sud, Université Paris-Saclay, 92290, Châtenay Malabry, France.
authors: Séverine
authors: S
authors: Péchiné
authors: Unité Bactéries Pathogènes et Santé (UBaPS), Faculté de Pharmacie, Université Paris-Sud, Université Paris-Saclay, 92290, Châtenay Malabry, France.
authors: Cécile
authors: C
authors: Denève-Larrazet
authors: Unité Bactéries Pathogènes et Santé (UBaPS), Faculté de Pharmacie, Université Paris-Sud, Université Paris-Saclay, 92290, Châtenay Malabry, France. anne.collignon@u-psud.fr.
authors: Anne
authors: A
authors: Collignon
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics / Q000378:metabolism; D018829:Adhesins, Bacterial / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D018938:Caco-2 Cells; D018834:Chaperonin 60 / Q000235:genetics / Q000378:metabolism; D002522:Chlorocebus aethiops; D016360:Clostridioides difficile / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D003015:Clostridium Infections / Q000382:microbiology / Q000473:pathology; D004768:Enterotoxins / Q000235:genetics / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005856:Germ-Free Life; D006801:Humans; D007422:Intestines / Q000382:microbiology / Q000473:pathology; D051379:Mice; D016253:Microscopy, Immunoelectron / Q000379:methods; D033902:Periplasmic Binding Proteins / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D014709:Vero Cells; D014774:Virulence
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D018834:Chaperonin 60; D004768:Enterotoxins; D005353:Fibronectins; D033902:Periplasmic Binding Proteins; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile; D036002:ADP Ribose Transferases; C410164:actin-specific ADP-ribosyltransferase, Clostridium
keywords: Adherence; Adhesins; Clostridium difficile; Intestinal colonization; Surface proteins