title: A plasma modified cellulose-chitosan porous membrane allows efficient DNA binding and provides antibacterial properties: A step towards developing a new DNA collecting card.
abstract: In forensic DNA analyses, biological specimens are collected and stored for subsequent recovery and analysis of DNA. A cost-effective and efficient DNA recovery approach is therefore a need. This study aims to produce a plasma modified cellulose-chitosan membrane (pCE-CS) that efficiently binds and retains DNA as a potential DNA collecting card. The pCE-CS membrane was produced by a phase separation of ionic liquid dissolving CE and CS in water with subsequent surface-modification by a two-step exposure of argon plasma and nitrogen gas. Through plasma modification, the pCE-CS membrane demonstrated better DNA retention after a washing process and higher rate of DNA recovery as compared with the original CE-CS membrane and the commercial FTA card. In addition, the pCE-CS membrane exhibited anti-bacterial properties against both Escherichia coli and Staphylococcus aureus. The results of this work suggest a potential function of the pCE-CS membrane as a DNA collecting card with a high recovery rate of captured DNA.
authors: Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Sasiwimon
authors: S
authors: Chumwangwapee
authors: Department of Physics, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand.
authors: Artit
authors: A
authors: Chingsungnoen
authors: School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. Electronic address: ssinee@sut.ac.th.
authors: Sineenat
authors: S
authors: Siri
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry; D001128:Argon; D002482:Cellulose / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000302:isolation & purification*; D005740:Gases; D006801:Humans; D008567:Membranes, Artificial; D009584:Nitrogen; D058626:Plasma Gases; D016062:Porosity; D013048:Specimen Handling / Q000295:instrumentation*
chemical_list: D000890:Anti-Infective Agents; D005740:Gases; D008567:Membranes, Artificial; D058626:Plasma Gases; D001128:Argon; D002482:Cellulose; D004247:DNA; D048271:Chitosan; D009584:Nitrogen
keywords: Antibacterial activity; Argon plasma; DNA collecting card; Forensics