title: Fluorescent imaging of Au(3+) in living cells with two new high selective Au(3+) probes.
abstract: Two new acetylenic compounds were designed and synthesized as the probes to realize the detection and recognition of Au(3+). The latent fluorophores was rearranged into the fluorescent coumarin derivatives with strong fluorescence by a gold ion-mediated hydroarylation reaction. In the meanwhile, the generated fluorescent substances were also isolated and characterized. The probes identified Au(3+) ions highly selectively over other biologically relevant metal ions, and detection concentration of Au(3+) was as low as 0.1µM or less. Fluorescent imaging of Au(3+) in living cells was also successfully demonstrated.
authors: Key Laboratory of Medicinal Chemistry, and Molecular Diagnosis of the Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China; KNC-Daishin Laboratories, Co. Ltd., Taicang 215400, PR China.
authors: Yutao
authors: Y
authors: Yang
authors: Key Laboratory of Medicinal Chemistry, and Molecular Diagnosis of the Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China.
authors: Bozan
authors: B
authors: Bai
authors: Key Laboratory of Medicinal Chemistry, and Molecular Diagnosis of the Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China.
authors: Ming
authors: M
authors: Jin
authors: Key Laboratory of Medicinal Chemistry, and Molecular Diagnosis of the Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China.
authors: Zhidong
authors: Z
authors: Xu
authors: Key Laboratory of Medicinal Chemistry, and Molecular Diagnosis of the Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China.
authors: Jinchao
authors: J
authors: Zhang
authors: Key Laboratory of Medicinal Chemistry, and Molecular Diagnosis of the Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China. Electronic address: liweihb@hotmail.com.
authors: Wei
authors: W
authors: Li
authors: Key Laboratory of Medicinal Chemistry, and Molecular Diagnosis of the Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China.
authors: Wenzhi
authors: W
authors: Xu
authors: KNC-Daishin Laboratories, Co. Ltd., Taicang 215400, PR China.
authors: Xiang
authors: X
authors: Wang
authors: Institute of Molecular Science, Shanxi University, Taiyuan 030006, PR China. Electronic address: yincx@sxu.edu.cn.
authors: Caixia
authors: C
authors: Yin
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006046:Gold / Q000032:analysis*; D006801:Humans; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence / Q000379:methods*; D015336:Molecular Probe Techniques; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013347:Subcellular Fractions / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D006046:Gold
keywords: Acetylenic compounds; Au(3+); Bioimaging; Fluorescent detection