title: Comparison of Two Commercial Automated Nucleic Acid Extraction and Integrated Quantitation Real-Time PCR Platforms for the Detection of Cytomegalovirus in Plasma.
abstract: Quantitation of cytomegalovirus (CMV) viral load in the transplant patients has become a standard practice for monitoring the response to antiviral therapy. The cut-off values of CMV viral load assays for preemptive therapy are different due to the various assay designs employed. To establish a sensitive and reliable diagnostic assay for preemptive therapy of CMV infection, two commercial automated platforms including m2000sp extraction system integrated the Abbott RealTime (m2000rt) and the Roche COBAS AmpliPrep for extraction integrated COBAS Taqman (CAP/CTM) were evaluated using WHO international CMV standards and 110 plasma specimens from transplant patients. The performance characteristics, correlation, and workflow of the two platforms were investigated. The Abbott RealTime assay correlated well with the Roche CAP/CTM assay (R2 = 0.9379, P<0.01). The Abbott RealTime assay exhibited higher sensitivity for the detection of CMV viral load, and viral load values measured with Abbott RealTime assay were on average 0.76 log10 IU/mL higher than those measured with the Roche CAP/CTM assay (P<0.0001). Workflow analysis on a small batch size at one time, using the Roche CAP/CTM platform had a shorter hands-on time than the Abbott RealTime platform. In conclusion, these two assays can provide reliable data for different purpose in a clinical virology laboratory setting.
authors: Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan.|Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
authors: Huey-Pin
authors: HP
authors: Tsai
authors: Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan.
authors: You-Yuan
authors: YY
authors: Tsai
authors: Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan.
authors: I-Ting
authors: IT
authors: Lin
authors: Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan.
authors: Pin-Hwa
authors: PH
authors: Kuo
authors: Department of Internal Medicine, National Cheng Kung University Hospital, Tainan, Taiwan.
authors: Tsai-Yun
authors: TY
authors: Chen
authors: Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan.
authors: Kung-Chao
authors: KC
authors: Chang
authors: Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan.|Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan.|Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan.|National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan.
authors: Jen-Ren
authors: JR
authors: Wang
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D012367:RNA, Viral / Q000097:blood; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D014180:Transplantation; D019562:Viral Load; D057188:Workflow
chemical_list: D012367:RNA, Viral