title: Effects of Diaceto-Dipropyl-Disulphide on Plasma Sialic Acid and Renal Tissue Thiol Levels in Alloxan Diabetic Rats.
abstract: BACKGROUND
  Plasma sialic acid levels are elevated in Diabetes Mellitus (DM) patients with proteinuria. Renal damage is mainly caused by free radicals that are excessively generated in DM. Thiols play an important role in the cellular antioxidative defence mechanisms mainly through thiol-disulphide exchange reaction. Diallyl disulphide, a garlic oil principle component, is known for its anti-diabetic properties. Its structural analogue, Diaceto-Dipropyl Disulphide (DADPDS), is a less toxic and more palatable disulphide and possesses similar anti-diabetic actions.
  
  
  OBJECTIVE
  This study was undertaken to assess the usefulness of DADPDS in prevention of de-sialation of Glomerular Basement Membrane (GBM) in alloxan diabetic rats and to assess effect of DADPDS on renal tissue thiol levels.
  
  
  METHODS
  Rats were divided into Normal, Diabetic and DADPDS treated diabetic groups. Diabetes was induced by intraperitoneal injection (IP) of alloxan. DADPDS was fed by gastric intubation. Plasma Sialic acid was determined by Ehrlich's method and renal tissue thiol levels by Nitroprusside reaction method.
  
  
  RESULTS
  This study showed a significant decrease (p<0.001) in plasma sialic acid, plasma glucose and renal tissue TBARS levels along with significant increase (p<0.001) in renal tissue thiol levels in DADPDS treated alloxan diabetic rats when compared to diabetic control rats.
  
  
  CONCLUSIONS
  Hence it may be concluded that DADPDS helps in preventing de-sialation of GBM in alloxan diabetic rats and improves renal tissue antioxidant defence mechanisms, may be through thiol-disulphide exchange reaction and thereby exhibits a possible clinical use in prevention of renal complications like diabetic nephropathy.
authors: Associate Professor, Department of Biochemistry, Vijayanagara Institute of Medical Sciences , Ballari, Karnataka, India .
authors: Vijay
authors: V
authors: Venkataiah
authors: Assistant Professor, Department of Biochemistry, DMWIMS , Waynad, Kerala, India .
authors: Vickram
authors: Professor, Department of Biochemistry, SSIMC&RC , Shivamogga, Karnataka, India .
authors: Kashinath Rattihalli
authors: KR
authors: Thirumalarao
authors: Tutor, Department of Biochemistry, Basaveshwara Medical College and Hospital , Chitradurga, Karnataka, India .
authors: Veena Gajana
authors: VG
authors: Raiker
authors: Assistant Professor, Department of Physiology, VIMS , Ballari. Karnataka, India .
authors: Sandhya Hanumanthappa
authors: SH
authors: Puttaswamy
keywords: Desialation, Sialidase; Diabetic nephropathy; Thiol-disulphide exchange reaction