title: Chronic reactive oxygen species exposure inhibits glucose uptake and causes insulin resistance in C2C12 myotubes.
abstract: Reactive oxygen species (ROS) is an important regulator in cellular signaling transduction, and many previous studies have indicated that acute ROS stimulation improves insulin sensitivity in skeletal muscle. In the study, we found that chronic ROS treatment caused serious insulin resistance in C2C12 myotubes. Glucose uptake and consumption assay indicated that pretreatment with 80 μM H2O2 for 2 h inhibited insulin-stimulated glucose uptake in C2C12 myotubes, and the reason for it, is that chronic H2O2 treatment decreased insulin-induced glucose transporter 4 (GLUT4) translocation from cell plasma to cell membrane. Moreover, Akt2 phosphorylation depended on insulin was reduced in C2C12 myotubes of chronic H2O2 treatment. Together, this study provides further demonstration that chronic ROS stress is associated with insulin resistance of skeletal muscle in the progression of type 2 diabetes.
authors: Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, China. Electronic address: dinghongw@126.com.
authors: Hongwen
authors: H
authors: Ding
authors: Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
authors: Baoli
authors: B
authors: Heng
authors: Education Centre of Micro-Morphology for Medicine, Jinan University, Guangzhou, China.
authors: Wenfang
authors: W
authors: He
authors: Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
authors: Liping
authors: L
authors: Shi
authors: Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
authors: Caiyong
authors: C
authors: Lai
authors: Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
authors: Long
authors: L
authors: Xiao
authors: Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
authors: Haolin
authors: H
authors: Ren
authors: Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
authors: Shijie
authors: S
authors: Mo
authors: Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
authors: Zexuan
authors: Z
authors: Su
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression; D005947:Glucose / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D007333:Insulin Resistance; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D021381:Protein Transport; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism
chemical_list: D051275:Glucose Transporter Type 4; D007328:Insulin; C495693:Slc2a4 protein, mouse; D006861:Hydrogen Peroxide; C494922:Akt2 protein, mouse; D051057:Proto-Oncogene Proteins c-akt; D005947:Glucose
keywords: Akt2; C2C12 myotubes; GLUT4; Glucose uptake; Insulin resistance; Reactive oxygen species