title: Binding-responsive catalysis of Taq DNA polymerase for the sensitive and selective detection of cell-surface proteins.
abstract: Here we develop a new method for the sensitive and selective detection of cell-surface proteins with an aptamer probe designed for binding-responsive catalysis of Taq DNA polymerase. Taking the biotin receptor as a model, the method allows the detection of target protein on surfaces of different types of cancer cells.
authors: Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, China. conezimint@shu.edu.cn.
authors: Zhuxin
authors: Z
authors: Wang
authors: Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, China. conezimint@shu.edu.cn and Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University, Shanghai 200444, China.
authors: Yifei
authors: Y
authors: Li
authors: Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, China. conezimint@shu.edu.cn.
authors: Peng
authors: P
authors: Han
authors: Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, China. conezimint@shu.edu.cn.
authors: Xiaoxia
authors: X
authors: Mao
authors: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. ym.yin@hotmail.com.
authors: Yongmei
authors: Y
authors: Yin
authors: Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, China. conezimint@shu.edu.cn.
authors: Ya
authors: Y
authors: Cao
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D045325:HCT116 Cells; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence / Q000379:methods; D019914:Taq Polymerase / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D008565:Membrane Proteins; D019914:Taq Polymerase