title: Chemical characterization and prebiotic activity of fructo-oligosaccharides from Stevia rebaudiana (Bertoni) roots and in vitro adventitious root cultures.
abstract: Stevia rebaudiana (Bertoni) is widely studied because of its foliar steviol glycosides. Fructan-type polysaccharides were recently isolated from its roots. Fructans are reserve carbohydrates that have important positive health effects and technological applications in the food industry. The objective of the present study was to isolate and characterize fructo-oligosaccharides (FOSs) from S. rebaudiana roots and in vitro adventitious root cultures and evaluate the potential prebiotic effect of these molecules. The in vitro adventitious root cultures were obtained using a roller bottle system. Chemical analyses (gas chromatography-mass spectrometry, (1)H nuclear magnetic resonance, and off-line electrospray ionization-mass spectrometry) revealed similar chemical properties of FOSs that were obtained from the different sources. The potential prebiotic effects of FOSs that were isolated from S. rebaudiana roots enhanced the growth of both bifidobacteria and lactobacilli, with strains specificity in their fermentation ability.
authors: Graduate Program in Pharmaceutical Sciences, State University of Maringá, Ave. Colombo 5790, 87.020-900, Maringá, Brazil. Electronic address: sanches_sheila@hotmail.com.
authors: Sheila Mara
authors: SM
authors: Sanches Lopes
authors: Department of Pharmacy, State University of Maringá, Ave. Colombo 5790, 87.020-900, Maringá, Brazil. Electronic address: mariane_fco@hotmail.com.
authors: Mariane Grigio
authors: MG
authors: Francisco
authors: Graduate Program in Pharmaceutical Sciences, State University of Maringá, Ave. Colombo 5790, 87.020-900, Maringá, Brazil. Electronic address: brunahigashi@hotmail.com.
authors: Bruna
authors: B
authors: Higashi
authors: Department of Chemistry, State University of Maringá, Ave. Colombo 5790, 87.020-900, Maringá, Brazil. Electronic address: rafaela.takako@gmail.com.
authors: Rafaela Takako Ribeiro
authors: RTR
authors: de Almeida
authors: Department of Microbiology and Technology, Dairy Research Institute, Ke Dvoru 12a, 160 00 Prague, Czech Republic. Electronic address: gabika.kunova@gmail.com.
authors: Gabriela
authors: G
authors: Krausová
authors: Department of Chemistry, State University of Maringá, Ave. Colombo 5790, 87.020-900, Maringá, Brazil. Electronic address: epilau@gmail.com.
authors: Eduardo Jorge
authors: EJ
authors: Pilau
authors: Program of Master in Health Promotion, University Center of Maringá, Ave. Guedner, 1610, 87.050-900, Maringá, Brazil. Electronic address: jose.goncalves@unicesumar.edu.br.
authors: José Eduardo
authors: JE
authors: Gonçalves
authors: Graduate Program in Pharmaceutical Sciences, State University of Maringá, Ave. Colombo 5790, 87.020-900, Maringá, Brazil; Department of Pharmacy, State University of Maringá, Ave. Colombo 5790, 87.020-900, Maringá, Brazil. Electronic address: racgoncalves@uem.br.
authors: Regina Aparecida Correia
authors: RAC
authors: Gonçalves
authors: Graduate Program in Pharmaceutical Sciences, State University of Maringá, Ave. Colombo 5790, 87.020-900, Maringá, Brazil; Department of Pharmacy, State University of Maringá, Ave. Colombo 5790, 87.020-900, Maringá, Brazil. Electronic address: ajboliveira@uem.br.
authors: Arildo José Braz de
authors: AJB
authors: Oliveira
mesh_terms: D009844:Oligosaccharides / Q000096:biosynthesis*; D059828:Plant Cells / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism*; D056692:Prebiotics; D036625:Stevia / Q000166:cytology / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D056692:Prebiotics
keywords: Adventitious root cultures; Agar (PubChem CID: 76645041); Ethanol (PubChem CID: 702); Fructo-oligosaccharides; Fructose (PubChem CID: 5984); Glucose (PubChem CID: 5793); Inulin from chicory (PubChem CID: 16219508); Prebiotic potential; Stevia rebaudiana; Sucrose (PubChem CID: 5988); Trifluoroacetic Acid (PubChem CID: 6422); Tween 80 (PubChem CID: 86289060); l-Cysteine Hydrochloride (PubChem CID: 60960); α-Naphthaleneacetic Acid (PubChem CID: 6862)