title: Coupling between the DEAD-box RNA helicases Ded1p and eIF4A.
abstract: Eukaryotic translation initiation involves two conserved DEAD-box RNA helicases, eIF4A and Ded1p. Here we show that S. cerevisiae eIF4A and Ded1p directly interact with each other and simultaneously with the scaffolding protein eIF4G. We delineate a comprehensive thermodynamic framework for the interactions between Ded1p, eIF4A, eIF4G, RNA and ATP, which indicates that eIF4A, with and without eIF4G, acts as a modulator for activity and substrate preferences of Ded1p, which is the RNA remodeling unit in all complexes. Our results reveal and characterize an unexpected interdependence between the two RNA helicases and eIF4G, and suggest that Ded1p is an integral part of eIF4F, the complex comprising eIF4G, eIF4A, and eIF4E.
authors: Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, United States.|Department of Biochemistry, Case Western Reserve University, Cleveland, United States.|School of Medicine, Case Western Reserve University, Cleveland, United States.
authors: Zhaofeng
authors: Z
authors: Gao
authors: Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, United States.|Department of Biochemistry, Case Western Reserve University, Cleveland, United States.|School of Medicine, Case Western Reserve University, Cleveland, United States.
authors: Andrea A
authors: AA
authors: Putnam
authors: Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, United States.|Department of Biochemistry, Case Western Reserve University, Cleveland, United States.|School of Medicine, Case Western Reserve University, Cleveland, United States.
authors: Heath A
authors: HA
authors: Bowers
authors: Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, United States.|Department of Biochemistry, Case Western Reserve University, Cleveland, United States.|School of Medicine, Case Western Reserve University, Cleveland, United States.
authors: Ulf-Peter
authors: UP
authors: Guenther
authors: Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, United States.|Department of Biochemistry, Case Western Reserve University, Cleveland, United States.|School of Medicine, Case Western Reserve University, Cleveland, United States.
authors: Xuan
authors: 0000-0002-3157-7537
authors: X
authors: Ye
authors: Department of Biology, University of Richmond, Richmond, United States.
authors: Audrey
authors: A
authors: Kindsfather
authors: Department of Biology, University of Richmond, Richmond, United States.
authors: Angela K
authors: AK
authors: Hilliker
authors: Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, United States.|Department of Biochemistry, Case Western Reserve University, Cleveland, United States.|School of Medicine, Case Western Reserve University, Cleveland, United States.
authors: Eckhard
authors: 0000-0001-7677-7412
authors: E
authors: Jankowsky
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D039601:Eukaryotic Initiation Factor-4A / Q000378:metabolism*; D039603:Eukaryotic Initiation Factor-4G / Q000378:metabolism*; D060066:Protein Interaction Maps; D012331:RNA, Fungal / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D039603:Eukaryotic Initiation Factor-4G; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D039601:Eukaryotic Initiation Factor-4A; C492152:DED1 protein, S cerevisiae; D053487:DEAD-box RNA Helicases
keywords: RNA; S. cerevisiae; biochemistry; helicase; none; translation