title: Micromonospora profundi sp. nov., isolated from deep marine sediment.
abstract: A novel actinobacterial strain, designated DS3010T, was isolated from a Black Sea marine sediment and characterized using a polyphasic approach. The strain was shown to have chemotaxonomic, morphological and phylogenetic properties consistent with classification as representing a member of the genus Micromonospora. Comparative 16S rRNA gene sequence studies showed that the strain was most closely related to the type strains of Micromonospora saelicesensis (99.5 %), Micromonospora chokoriensis (99.4 %) and Micromonospora violae (99.3 %). Similarly, a corresponding analysis based on partial gyrB gene sequences showed that it formed a distinct phyletic branch in a subclade that included the type strains of Micromonosporazamorensis, 'Micromonospora zeae', 'Micromonospora jinlongensis', M. saelicesensis and Micromonospora lupini. DS3010T was distinguished from its closest phylogenetic neighbours by low levels of DNA-DNA relatedness and by a combination of chemotaxonomic and phenotypic properties. On the basis of these data, it is proposed that the isolate should be assigned to the genus Micromonospora as Micromonospora profundi sp. nov. with isolate DS3010T (=DSM 45981T=KCTC 29243T) as the type strain.
authors: Department of Bioengineering, Faculty of Engineering and Architecture, Sinop University, Nasuhbasoglu-Sinop 57000, Turkey.
authors: Aysel
authors: A
authors: Veyisoglu
authors: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors: Lorena
authors: L
authors: Carro
authors: Science Teaching Programme, Gazi Faculty of Education, Gazi University, Ankara, Turkey.
authors: Demet
authors: D
authors: Cetin
authors: Anadolu University, Faculty of Science, Biology Department, Eskisehir 26470, Turkey.
authors: Kiymet
authors: K
authors: Guven
authors: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, Braunschweig 38124, Germany.
authors: Cathrin
authors: C
authors: Spröer
authors: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, Braunschweig 38124, Germany.
authors: Gabriele
authors: G
authors: Pötter
authors: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors: Hans-Peter
authors: HP
authors: Klenk
authors: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, Kurupelit-Samsun 55139, Turkey.
authors: Nevzat
authors: N
authors: Sahin
authors: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors: Michael
authors: M
authors: Goodfellow
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D059038:Black Sea; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D019015:Geologic Sediments / Q000382:microbiology*; D008847:Micromonospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S