title: Mild heat treatments induce long-term changes in metabolites associated with energy metabolism in Drosophila melanogaster.
abstract: Heat-induced hormesis, the beneficial effect of mild heat-induced stress, increases the average lifespan of many organisms. Yet little is known about the mechanisms underlying this effect. We used nuclear magnetic resonance spectroscopy to investigate the long-term effects of repeated mild heat treatments on the metabolome of male Drosophila melanogaster. 10 days after the heat treatment, metabolic aging appears to be slowed down, and a treatment response with 40 % higher levels of alanine and lactate and lower levels of aspartate and glutamate were measured. All treatment effects had disappeared 16 days later. Metabolic reprogramming has been associated with the life extending effects of dietary restriction. The metabolite changes induced by the hormetic treatment suggest that the positive effects might not be limited to the repair pathways induced, but that there also is a change in energy metabolism. A possible direct link between changes in energy metabolism and heat induced increase in Hsp70 expression is discussed.
authors: Department of Molecular Biology and Genetics, Center for Quantitative Genetics and Genomics, Blichers Allé 20, 8830, Tjele, Denmark.|Department of Bioscience, Aarhus University, Ny Munkegade, 114, 8000, Aarhus C, Denmark.
authors: Pernille
authors: P
authors: Sarup
authors: Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, Aarhus University, Gustav Wieds Vej 14, 8000, Aarhus C, Denmark.|Department of Food Science, Aarhus University, Blichers Allé 20, P.O. Box 50, 8830, Tjele, Denmark.|Arla Foods Strategic Innovation Centre, Rørdrumvej 2, 8220, Brabrand, Denmark.
authors: Simon Metz Mariendal
authors: SM
authors: Petersen
authors: Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, Aarhus University, Gustav Wieds Vej 14, 8000, Aarhus C, Denmark.
authors: Niels Chr
authors: NC
authors: Nielsen
authors: Department of Bioscience, Aarhus University, Ny Munkegade, 114, 8000, Aarhus C, Denmark. volker.loeschcke@bios.au.dk.
authors: Volker
authors: V
authors: Loeschcke
authors: Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, Aarhus University, Gustav Wieds Vej 14, 8000, Aarhus C, Denmark.|Department of Biomedical Sciences, University of Copenhagen, 2200, Copenhagen N, Denmark.
authors: Anders
authors: A
authors: Malmendal
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000502:physiology*; D004734:Energy Metabolism / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism*; D018869:Heat-Shock Response / Q000502:physiology*; D008297:Male; D055442:Metabolome / Q000502:physiology; D000071436:Thermotolerance / Q000502:physiology*
chemical_list: D029721:Drosophila Proteins; D050884:HSP72 Heat-Shock Proteins
keywords: Energy metabolism; Heat stress; Hormesis; Hsp70; Longevity; Metabolomics; NMR