title: Biocompatibility of plasma-treated poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanofiber mats modified by silk fibroin for bone tissue regeneration.
abstract: The objective of this study was to produce biocompatible plasma-treated and silk-fibroin (SF) modified poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofiber mats. The mats were plasma-treated using O2 or N2 gas to increase their hydrophilicity followed by SF immobilization for the improvement of biocompatibility. Contact angle measurements and SEM showed increased hydrophilicity and no disturbed morphology, respectively. Cell proliferation assay revealed that SF modification together with N2 plasma (PS/N2) promoted higher osteoblastic (SaOs-2) cell viability. Although, O2 plasma triggered more mineral formation on the mats, it showed poor cell viability. Consequently, the PS/N2 nanofiber mats would be a potential candidate for bone tissue engineering applications.
authors: Biomedical Technologies Department, Graduate School of Natural and Applied Sciences, Dokuz Eylul University, Izmir, Turkey.
authors: Irem
authors: I
authors: Unalan
authors: Metallurgical and Materials Engineering Department, Faculty of Engineering, Dokuz Eylul University, Izmir, Turkey.
authors: Oylum
authors: O
authors: Colpankan
authors: Metallurgical and Materials Engineering Department, Faculty of Engineering, Dokuz Eylul University, Izmir, Turkey. Electronic address: aylin.albayrak@deu.edu.tr.
authors: Aylin Ziylan
authors: AZ
authors: Albayrak
authors: Biomedical Technologies Department, Graduate School of Natural and Applied Sciences, Ege University, Izmir, Turkey.
authors: Cansu
authors: C
authors: Gorgun
authors: Biomedical Technologies Department, Graduate School of Natural and Applied Sciences, Ege University, Izmir, Turkey; Bioengineering Department, Faculty of Engineering, Ege University, Izmir, Turkey.
authors: Aylin Sendemir
authors: AS
authors: Urkmez
mesh_terms: D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry*; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology; D058626:Plasma Gases / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D023822:Tissue Engineering; D000094122:Polyhydroxybutyrates
chemical_list: D018786:Bone Substitutes; D005349:Fibroins; D058626:Plasma Gases; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates
keywords: Electrospinning; Nanofiber; PHBV; Plasma surface modification; Silk fibroin