title: Laterally Confined Microfluidic Patterning of Cells for Engineering Spatially Defined Vascularization.
abstract: A biofabrication strategy for creating planar multiscale protein, hydrogel, and cellular patterns, and simultaneously generating microscale topographical features is developed that laterally confines the patterned cells and direct their growth in cell permissive hydrogels.
authors: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Department of Mechanical Engineering, University of British Columbia, Kelowna, BC, Canada, V1V 1V7.
authors: Hojatollah
authors: H
authors: Rezaei Nejad
authors: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Department of Mechanical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors: Zahra
authors: Z
authors: Goli Malekabadi
authors: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors: Mehdi
authors: M
authors: Kazemzadeh Narbat
authors: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Department of Chemical Engineering, Northeastern University, Boston, MA, 02115-5000, USA.|Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
authors: Nasim
authors: N
authors: Annabi
authors: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
authors: Pooria
authors: P
authors: Mostafalu
authors: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors: Farhang
authors: F
authors: Tarlan
authors: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
authors: Yu Shrike
authors: YS
authors: Zhang
authors: Department of Mechanical Engineering, University of British Columbia, Kelowna, BC, Canada, V1V 1V7.
authors: Mina
authors: M
authors: Hoorfar
authors: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA. atamayol@partners.org.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. atamayol@partners.org.|Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA. atamayol@partners.org.
authors: Ali
authors: A
authors: Tamayol
authors: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA. alik@bwh.harvard.edu.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. alik@bwh.harvard.edu.|Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA. alik@bwh.harvard.edu.|Department of Physics, King Abdulaziz University, Jeddah, 21569, Saudi Arabia. alik@bwh.harvard.edu.|Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Seoul, 05029, Republic of Korea. alik@bwh.harvard.edu.
authors: Ali
authors: A
authors: Khademhosseini
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology; D044085:Microfluidics / Q000379:methods*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D013194:Staining and Labeling; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000199:Actins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: capillary-driven microfluidics; cell patterning; hydrogels; microfabrication; vascularization