title: Synthesized Peptides from Yam Dioscorin Hydrolysis in Silico Exhibit Dipeptidyl Peptidase-IV Inhibitory Activities and Oral Glucose Tolerance Improvements in Normal Mice.
abstract: RRDY, RL, and DPF were the top 3 of 21 peptides for inhibitions against dipeptidyl peptidase-IV (DPP-IV) from the pepsin hydrolysis of yam dioscorin in silico and were further investigated in a proof-of-concept study in normal ICR mice for regulating glucose metabolism by the oral glucose tolerance test (OGTT). The sample or sitagliptin (positive control) was orally administered by a feeding gauge; 30 min later, the glucose loads (2.5 g/kg) were performed. RRDY, yam dioscorin, or sitagliptin preload, but not DPF, lowered the area under the curve (AUC0-120) of blood glucose and DPP-IV activity and elevated the AUC0-120 of blood insulin, which showed significant differences compared to control (P < 0.05 or 0.001). These results suggested that RRDY and yam dioscorin might be beneficial in glycemic control in normal mice and need further investigations in diabetic animal models.
authors: Yin-Shiou
authors: YS
authors: Lin
authors: Department of Health and Creative Vegetarian Science, Fo Guang University , Yilan County 262, Taiwan.
authors: Chuan-Hsiao
authors: CH
authors: Han
authors: Shyr-Yi
authors: SY
authors: Lin
authors: Wen-Chi
authors: WC
authors: Hou
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D027742:Dioscorea / Q000737:chemistry*; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D005951:Glucose Tolerance Test; D006868:Hydrolysis; D007328:Insulin / Q000097:blood / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010455:Peptides / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D000068900:Sitagliptin Phosphate / Q000737:chemistry
chemical_list: D001786:Blood Glucose; D054873:Dipeptidyl-Peptidase IV Inhibitors; D007328:Insulin; D010455:Peptides; D010940:Plant Proteins; C095057:dioscorin protein, Dioscorea; D005947:Glucose; D000068900:Sitagliptin Phosphate
keywords: blood insulin; blood sugar; dipeptidyl peptidase-IV (DPP-IV); oral glucose tolerance test (OGTT); sitagliptin; yam dioscorin