title: Porphyromonas loveana sp. nov., isolated from the oral cavity of Australian marsupials.
abstract: An obligatory anaerobic, Gram-stain-negative coccobacillus with black-pigmented colonies was isolated from the oral cavity of selected Australian marsupial species. Phenotypic and molecular criteria showed that this bacterium was a distinct species within the genus Porphyromonas, and was closely related to Porphyromonas gingivalis and Porphyromonas gulae. This putative novel species and P. gulae could be differentiated from P. gingivalis by catalase activity. Further characterization by multi-locus enzyme electrophoresis of glutamate dehydrogenase and malate dehydrogenase enzyme mobility and matrix-assisted laser desorption ionization time-of-flight MS showed that this putative novel species could be differentiated phenotypically from P. gingivalis and P. gulae. Definitive identification by 16S rRNA gene sequencing showed that this bacterium belonged to a unique monophyletic lineage, phylogenetically distinct from P. gingivalis (94.9 % similarity) and P. gulae (95.5 %). This also was supported by 16S-23S rRNA intergenic spacer region and glutamate dehydrogenase gene sequencing. A new species epithet, Porphyromonas loveana sp. nov., is proposed for this bacterium, with DSM 28520T (=NCTC 13658T=UQD444T=MRK101T), isolated from a musky rat kangaroo, as the type strain.
authors: School of Veterinary Science, Faculty of Science, The University of Queensland, Gatton, Queensland 4343, Australia.
authors: Philip S
authors: PS
authors: Bird
authors: School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, South Australia 5371, Australia.
authors: Darren J
authors: DJ
authors: Trott
authors: ARC Centre of Excellence in Plant Cell Walls, Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Queensland 4072, Australia.
authors: Deirdre
authors: D
authors: Mikkelsen
authors: School of Population Health, Faculty of Medicine and Biomedical Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia.
authors: Gabriel J
authors: GJ
authors: Milinovich
authors: QIMR Berghofer, Royal Brisbane Hospital, Herston, Queensland 4029, Australia.
authors: Kristine M
authors: KM
authors: Hillman
authors: Science and Engineering Faculty, Gardens Point Campus, Queensland University of Technology, Brisbane, Queensland 4000, Australia.
authors: Paul C
authors: PC
authors: Burrell
authors: School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia.
authors: Linda L
authors: LL
authors: Blackall
mesh_terms: D000818:Animals; D001315:Australia; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005969:Glutamate Dehydrogenase / Q000235:genetics; D008394:Marsupialia / Q000382:microbiology*; D009055:Mouth / Q000382:microbiology*; D010802:Phylogeny; D010858:Pigmentation; D017861:Porphyromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D005969:Glutamate Dehydrogenase