title: Epigenetic modulation on cat-cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A.
abstract: This study aimed to determine the acetylation at lysine 9/18/23 of histone H3 (H3K9ac/H3K18ac/H3K23ac; H3K9/18/23 ac) and the di-methylation at lysine 9 of histone H3 (H3K9me2) during early embryogenesis among trichostatin A (TSA)-treated interspecies somatic cell nuclear transfer (iSCNT) cat-cow (TSA-iSCNT) embryos, TSA-untreated iSCNT cat-cow control (control) embryos and bovine in vitro fertilization (IVF) embryos, because TSA-iSCNT embryos can develop to blastocysts. Compared to the control embryos, higher expressions of H3K9/18/23 ac were observed in TSA-iSCNT embryos and IVF embryos at most following stages (2 h post-fusion / post-insemination (PF/PI) to eight-cell stage). At 6 h PF/PI the expression of H3K9/23 ac in TSA-iSCNT embryos and IVF embryos were lower than those in control embryos, and the expression of H3K18ac was no difference among the three groups. The expression of H3K9/23 ac increased in TSA-iSCNT embryos and IVF embryos at pronuclear (PN) stages. The expression of H3K9me2 in TSA-iSCNT embryos resembled that of IVF embryos at 2 h PF/PI to PN stages, and these expression levels were greater than those of control embryos. These results suggest that treatment of iSCNT embryos with TSA modifies the patterns of histone modification at certain lysine residues in a manner that is comparable with that seen in IVF during early embryogenesis.
authors: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.|Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok, Thailand.
authors: Manita
authors: M
authors: Wittayarat
authors: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors: Yoko
authors: Y
authors: Sato
authors: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors: Lanh Thi Kim
authors: LT
authors: Do
authors: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok, Thailand.
authors: Kaywalee
authors: K
authors: Chatdarong
authors: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok, Thailand.
authors: Theerawat
authors: T
authors: Tharasanit
authors: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok, Thailand.
authors: Mongkol
authors: M
authors: Techakumphu
authors: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors: Masayasu
authors: M
authors: Taniguchi
authors: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.|Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry (Ishii campus), Tokushima University, Tokushima, Japan.
authors: Takeshige
authors: T
authors: Otoi
mesh_terms: D000107:Acetylation; D000818:Animals; D002415:Cats; D002417:Cattle; D000067470:Cellular Reprogramming Techniques; D047108:Embryonic Development; D044127:Epigenesis, Genetic / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro; D006657:Histones / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008239:Lysine; D008745:Methylation; D053652:Nuclear Transfer Techniques; D013045:Species Specificity
chemical_list: D006657:Histones; D006877:Hydroxamic Acids; C012589:trichostatin A; D008239:Lysine
keywords: acetylation; iSCNT; mammal; methylation; reprogramming