title: Cubitanoids and Cembranoids from the Soft Coral Sinularia nanolobata.
abstract: Two new cubitanoids, nanoculones A and B (1 and 2), and three new cembranoids, nanolobols A-C (3-5), as well as six known compounds, calyculone I (6), sinulariuol A (7), sinulariols C, D, H, and J (8-11), were isolated from the soft coral Sinularia nanolobata, collected off the coast of the eastern region of Taiwan. Their structures were elucidated on the basis of extensive spectroscopic analysis. Cytotoxicity of compounds 1-11 was evaluated. The nitric oxide (NO) inhibitory activity of selected compounds was further measured by assay of lipopolysaccharide (LPS)-stimulated NO production in activated RAW264.7 cells. The results showed that none of 1-11 exhibited cytotoxicity against the tested cancer cell lines, whereas compound 8 was found to significantly reduce NO production.
authors: School of Pharmacy, China Medical University, Taichung 404, Taiwan. chchao@mail.cmu.edu.tw.|Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan. chchao@mail.cmu.edu.tw.
authors: Chih-Hua
authors: CH
authors: Chao
authors: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan. eleahant111@yahoo.com.tw.
authors: Chia-Yun
authors: CY
authors: Wu
authors: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan. huangcy@mail.nsysu.edu.tw.
authors: Chiung-Yao
authors: CY
authors: Huang
authors: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan. wanghc@kmu.edu.tw.|Ph.D. Program in Translational Medicine, College of Medicine and Ph.D. Program in Toxicology, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. wanghc@kmu.edu.tw.|Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. wanghc@kmu.edu.tw.
authors: Hui-Chun
authors: HC
authors: Wang
authors: Institute of Oceanography, National Taiwan University, Taipei 112, Taiwan. corallab@ntu.edu.tw.
authors: Chang-Feng
authors: CF
authors: Dai
authors: School of Pharmacy, China Medical University, Taichung 404, Taiwan. yachwu@mail.cmu.edu.tw.|Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan. yachwu@mail.cmu.edu.tw.|Center for Molecular Medicine, China Medical University Hospital, Taichung 404, Taiwan. yachwu@mail.cmu.edu.tw.
authors: Yang-Chang
authors: YC
authors: Wu
authors: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan. sheu@mail.nsysu.edu.tw.|Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. sheu@mail.nsysu.edu.tw.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan. sheu@mail.nsysu.edu.tw.|Frontier Center for Ocean Science and Technology, National Sun Yat-sen University, Kaohsiung 804, Taiwan. sheu@mail.nsysu.edu.tw.
authors: Jyh-Horng
authors: JH
authors: Sheu
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D019073:HT29 Cells; D006801:Humans; D020014:K562 Cells; D008070:Lipopolysaccharides / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism*; D000067996:RAW 264.7 Cells
chemical_list: D000970:Antineoplastic Agents; D004224:Diterpenes; D008070:Lipopolysaccharides; D009569:Nitric Oxide
keywords: Sinularia; cubitane; nanoculones A and B; nanolobols A–C