title: Mitochondrial function assessed by 31P MRS and BOLD MRI in non-obese type 2 diabetic rats.
abstract: The study aims to characterize age-associated changes in skeletal muscle bioenergetics by evaluating the response to ischemia-reperfusion in the skeletal muscle of the Goto-Kakizaki (GK) rats, a rat model of non-obese type 2 diabetes (T2D). (31)P magnetic resonance spectroscopy (MRS) and blood oxygen level-dependent (BOLD) MRI was performed on the hindlimb of young (12 weeks) and adult (20 weeks) GK and Wistar (control) rats. (31)P-MRS and BOLD-MRI data were acquired continuously during an ischemia and reperfusion protocol to quantify changes in phosphate metabolites and muscle oxygenation. The time constant of phosphocreatine recovery, an index of mitochondrial oxidative capacity, was not statistically different between GK rats (60.8 ± 13.9 sec in young group, 83.7 ± 13.0 sec in adult group) and their age-matched controls (62.4 ± 11.6 sec in young group, 77.5 ± 7.1 sec in adult group). During ischemia, baseline-normalized BOLD-MRI signal was significantly lower in GK rats than in their age-matched controls. These results suggest that insulin resistance leads to alterations in tissue metabolism without impaired mitochondrial oxidative capacity in GK rats.
authors: Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio Case Center for Imaging Research, Case Western Reserve University, Cleveland, Ohio.
authors: Yuchi
authors: Y
authors: Liu
authors: Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio Case Center for Imaging Research, Case Western Reserve University, Cleveland, Ohio.
authors: Xunbai
authors: X
authors: Mei
authors: Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio.
authors: Jielei
authors: J
authors: Li
authors: Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio Department of Electrical and Computer Engineering and Biomedical Engineering Institute, Old Dominion University, Norfolk, Virginia.
authors: Nicola
authors: N
authors: Lai
authors: Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio Case Center for Imaging Research, Case Western Reserve University, Cleveland, Ohio Department of Radiology, Case Western Reserve University, Cleveland, Ohio Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio xin.yu@case.edu.
authors: Xin
authors: X
authors: Yu
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000000981:diagnostic imaging* / Q000378:metabolism*; D004195:Disease Models, Animal; D006863:Hydrogen-Ion Concentration; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000000981:diagnostic imaging* / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism
chemical_list: D010761:Phosphorus Radioisotopes; D010725:Phosphocreatine
keywords: 31P magnetic resonance spectroscopy; blood oxygen level‐dependent MRI; ischemia reperfusion; mitochondrial oxidative capacity