title: Comparison of differents methods of sperm selection of llama raw semen.
abstract: The objective of this study was to compare the efficiency of different sperm selection methods applied to the same llama ejaculate. Four treatments were compared: two variants of the swim up technique (with and without seminal plasma), and two different colloids, Androcoll-E-Large and Percoll(®). Using electroejaculation, 21 semen samples were obtained from 7 llama males (n=7, r=3). The ejaculates were incubated in a solution of 0.1% collagenase, to decrease thread formation, and then split into 4 aliquots: one aliquot was layered over a column of Androcoll-E-Large (SLC) and the second over a column of Percoll (45%). The third aliquot was deposited in a tube with culture medium and was incubated at a 45° angle for 30min at 37°C (SU1). The last aliquot was centrifuged to separate the spermatozoa and seminal plasma. The sperm pellet obtained was resuspended, and transferred to a tube with culture medium which was incubated at an angle of 45° for 30min at 37°C (SU2). Both aliquots SLC and P showed higher proportions of progressive motility and plasma membrane functionality (p≤0.05) than raw semen. There were no significant differences (p>0.05) in sperm viability and in normal spermatozoa between raw semen and treatments. Nevertheless, only SLC did not have a significant increase of bent tails. In conclusion SLC centrifugation would be the method of choice for selecting llama spermatozoa.
authors: Cátedra de Teriogenología, Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Facultad de Ciencias Veterinarias Universidad de Buenos Aires, Argentina.
authors: R
authors: R
authors: Santa Cruz
authors: Cátedra de Física Biológica, Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Facultad de Ciencias Veterinarias Universidad de Buenos Aires, Argentina. Electronic address: smgiulia@gmail.com.
authors: S M
authors: SM
authors: Giuliano
authors: Cátedra de Estadística, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Argentina.
authors: M C
authors: MC
authors: Gambarotta
authors: Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors: J M
authors: JM
authors: Morrell
authors: Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors: M C
authors: MC
authors: Abraham
authors: Cátedra de Teriogenología, Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Facultad de Ciencias Veterinarias Universidad de Buenos Aires, Argentina.
authors: M H
authors: MH
authors: Miragaya
authors: Cátedra de Teriogenología, Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Facultad de Ciencias Veterinarias Universidad de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors: M I
authors: MI
authors: Carretero
mesh_terms: D000818:Animals; D002161:Camelids, New World / Q000502:physiology*; D002469:Cell Separation / Q000379:methods*; D008297:Male; D055101:Semen Analysis / Q000662:veterinary; D012662:Semen Preservation / Q000662:veterinary; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology
keywords: Androcoll; Llama; Percoll; Sperm selection; Swim up