title: Long-term culture of human odontoma-derived cells with a Rho kinase inhibitor.
abstract: Because of cellular senescence/apoptosis, no effective culture systems are available to maintain replication of cells from odontogenic tumors especially for odontoma, and, thus, the ability to isolate human odontoma-derived cells (hODCs) for functional studies is needed. The current study was undertaken to develop an approach to isolate hODCs and fully characterize the cells in vitro. The hODCs were cultured successfully with a Rho-associated protein kinase inhibitor (Y-27632) for an extended period with stabilized lengths of the telomeres to sustain a similar phenotype/property as the primary tumoral cells. While the hODCs showed stable long-term expansion with expression of major dental epithelial markers including dentin sialophosphoprotein (DSPP) even in the three-dimensional microenvironment, they lack the specific markers for the characteristics of stem cells. Moreover, cells from dental pulp showed significant up-regulation of DSPP when co-cultured with the hODCs, while control fibroblasts with the hODCs did not. Taken together, we propose that the hODCs can be isolated and expanded over the long term with Y-27632 to investigate not only the development of the hODCs but also other types of benign human tumors.
authors: Department of Oral Science, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8670, Japan; Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8677, Japan. Electronic address: uzawak@faculty.chiba-u.jp.
authors: Katsuhiro
authors: K
authors: Uzawa
authors: Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8677, Japan.
authors: Atsushi
authors: A
authors: Kasamatsu
authors: Department of Oral Science, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8670, Japan.
authors: Tomoaki
authors: T
authors: Saito
authors: Department of Oral Science, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8670, Japan.
authors: Toshikazu
authors: T
authors: Takahara
authors: Department of Oral Science, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8670, Japan.
authors: Yasuyuki
authors: Y
authors: Minakawa
authors: Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8677, Japan.
authors: Kazuyuki
authors: K
authors: Koike
authors: Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8677, Japan.
authors: Masanobu
authors: M
authors: Yamatoji
authors: Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8677, Japan.
authors: Dai
authors: D
authors: Nakashima
authors: Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8677, Japan.
authors: Morihiro
authors: M
authors: Higo
authors: Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8677, Japan.
authors: Yosuke
authors: Y
authors: Sakamoto
authors: Department of Medical Oncology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8670, Japan.
authors: Masashi
authors: M
authors: Shiiba
authors: Department of Oral Science, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8670, Japan; Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8677, Japan.
authors: Hideki
authors: H
authors: Tanzawa
mesh_terms: D000577:Amides; D017209:Apoptosis / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects; D018920:Coculture Techniques; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009805:Odontogenesis / Q000187:drug effects / Q000235:genetics; D009810:Odontoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011725:Pyridines; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D059505:Telomere Homeostasis / Q000187:drug effects; D013997:Time Factors; D014407:Tumor Cells, Cultured; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000099311:Dentin Sialophosphoprotein
chemical_list: D000577:Amides; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D047428:Protein Kinase Inhibitors; D011725:Pyridines; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D019098:Telomerase; D054460:rho-Associated Kinases; D000099311:Dentin Sialophosphoprotein; C108830:Y 27632; C509186:TERT protein, human
keywords: Dentin sialophosphoprotein; Long-term culture; Mineralization; Odontoma; Rho kinase inhibitor; Telomere