title: An Adenovirus Vaccine Expressing Ebola Virus Variant Makona Glycoprotein Is Efficacious in Guinea Pigs and Nonhuman Primates.
abstract: A licensed vaccine against Ebola virus (EBOV) remains unavailable, despite >11 000 deaths from the 2014-2016 outbreak of EBOV disease in West Africa. Past studies have shown that recombinant vaccine viruses expressing EBOV glycoprotein (GP) are able to protect nonhuman primates (NHPs) from a lethal EBOV challenge. However, these vaccines express the viral GP-based EBOV variants found in Central Africa, which has 97.3% amino acid homology to the Makona variant found in West Africa. Our previous study showed that a recombinant adenovirus serotype 5 (Ad5)-vectored vaccine expressing the Makona EBOV GP (MakGP) was safe and immunogenic during clinical trials in China, but it is unknown whether the vaccine protects against EBOV infection. Here, we demonstrate that guinea pigs immunized with Ad5-MakGP developed robust humoral responses and were protected against exposure to guinea pig-adapted EBOV. Ad5-MakGP also elicited specific B- and T-cell immunity in NHPs and conferred 100% protection when animals were challenged 4 weeks after immunization. These results support further clinical development of this candidate and highlight the utility of Ad5-MakGP as a prophylactic measure in future outbreaks of EBOV disease.
authors: Beijing Institute of Biotechnology.
authors: Shipo
authors: S
authors: Wu
authors: Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
authors: Andrea
authors: A
authors: Kroeker
authors: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
authors: Gary
authors: G
authors: Wong
authors: Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
authors: Shihua
authors: S
authors: He
authors: Beijing Institute of Biotechnology.
authors: Lihua
authors: L
authors: Hou
authors: Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
authors: Jonathan
authors: J
authors: Audet
authors: Institute of Infectious Disease, Henan Centre for Disease Control, Zhengzhou.
authors: Haiyan
authors: H
authors: Wei
authors: Beijing Institute of Biotechnology.
authors: Zhe
authors: Z
authors: Zhang
authors: Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada.
authors: Lisa
authors: L
authors: Fernando
authors: Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada.
authors: Geoff
authors: G
authors: Soule
authors: Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada.
authors: Kaylie
authors: K
authors: Tran
authors: Institute of Viral Disease, China Center for Disease Control and Prevention, Beijing.
authors: Shengli
authors: S
authors: Bi
authors: Tianjin CanSino Biotechnology, China.
authors: Tao
authors: T
authors: Zhu
authors: Tianjin CanSino Biotechnology, China.
authors: Xuefeng
authors: X
authors: Yu
authors: Beijing Institute of Biotechnology.
authors: Wei
authors: W
authors: Chen
authors: Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
authors: Xiangguo
authors: X
authors: Qiu
mesh_terms: D062705:Adenovirus Vaccines / Q000276:immunology*; D000350:Africa, Central; D000354:Africa, Western; D000818:Animals; D002681:China; D046129:Ebola Vaccines / Q000276:immunology*; D029043:Ebolavirus / Q000276:immunology*; D005822:Genetic Vectors; D006023:Glycoproteins / Q000276:immunology; D006168:Guinea Pigs; D019142:Hemorrhagic Fever, Ebola / Q000517:prevention & control* / Q000821:virology; D006801:Humans; D007114:Immunization; D011323:Primates; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D062705:Adenovirus Vaccines; D046129:Ebola Vaccines; D006023:Glycoproteins; D014764:Viral Proteins
keywords: Ebola virus; adenovirus virus; guinea pigs; nonhuman primates; vaccine