title: Intervention effect of pinelliae decoction for purging stomach-fire on malignant transformation of bone marrow mesenchymal stem cells in the gastric cancer microenvironment.
abstract: OBJECTIVE
  The study aimed to simulate the microenvironment of gastric cancer to promote the malignant transformation of bone marrow mesenchymal stem cells (BMSCs) and further evaluate the effect of Pinelliae Decoction for Purging Stomach-Fire and its disassembled prescriptions on BMSCs.
  
  
  METHODS
  Transwell co-culture was performed on the human gastric cancer cell strains BGC-823 and BMSCs to simulate the microenvironment of gastric cancer. The drug-containing serum prepared by Pinelliae Decoction for Purging Stomach-Fire and its disassembled prescriptions was used, and its influence on BMSCs with malignant transformation was observed.
  
  
  RESULTS
  BMSCs were harvested successfully from the rat bone marrow, and flow cytometer identification indicated that CD44+/CD34- cells accounted for 70.64%. The co-culture of BGC-823 cells can induce malignant transformation of BMSCs. And the drug-containing serum can induce G2 phase arrest, inhibit cell proliferation, simultaneously inhibit TERT and c-myc expression, lower the cellular ability of chemotactic migration, inhibit the tumor-forming ability of BGC-823 in nude rats and promote the tumor apoptosis.
  
  
  CONCLUSIONS
  The effective components of Pinelliae Decoction for Purging Stomach-Fire in gastric cancer treatment are pinelliae and dried ginger, and the main acting mechanism is to inhibit tumor cell proliferation and chemotactic migration and promote apoptosis.
authors: Basic Medical College, Gansu University of Chinese Medicine Lanzhou 730000, China.
authors: Xi-Ping
authors: XP
authors: Liu
authors: Basic Medical College, Gansu University of Chinese Medicine Lanzhou 730000, China.
authors: Hai-Xia
authors: HX
authors: Ming
authors: Basic Medical College, Gansu University of Chinese Medicine Lanzhou 730000, China.
authors: Pei-Qing
authors: PQ
authors: Li
keywords: Pinelliae decoction; bone marrow mesenchymal stem cells; gastric cancer; purging stomach-fire