title: The protective role of p72 in doxorubicin‑induced cardiomyocytes injury in vitro.
abstract: p72 (probable ATP-dependent RNA helicase DDX17) belongs to the DEAD‑box RNA helicase family. p72 is important in RNA processing. Thus, the role of p72 in doxorubicin (DOX)‑induced cardiomyocyte injury was investigated in the present study. The changes in p72 expression levels were studied in cultured neonatal cardiomyocytes and p72 overexpression was induced using adenovirus vectors. To investigate the production of reactive oxygen species (ROS), dihydroethidium staining was conducted. TUNEL and Hoechst staining were used to indicate cell apoptosis. Microarrays were used to determine the altered expression of microRNAs. In DOX‑induced cardiomyocyte injury, the protein expression level of p72 was reduced. Overexpression of p72 protected cardiomyocytes from DOX‑induced ROS production and cell apoptosis. p72 reduced the activation of estrogen receptor α (ERα), thereby reducing DOX‑induced cell apoptosis. The present study indicated that p72 exerts a protective effect against DOX‑induced cell apoptosis via inhibition of ERα activation, indicating this may be a potential target of therapy for cardiac injury.
authors: Department of Cardiovascular Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors: Bin
authors: B
authors: Lin
authors: Department of Cardiovascular Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors: Feng
authors: F
authors: Wang
authors: Department of Cardiovascular Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors: Jiaxiang
authors: J
authors: Wang
authors: Department of Cardiovascular Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors: Chengyang
authors: C
authors: Xu
authors: Department of Cardiovascular Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors: Hui
authors: H
authors: Zhao
authors: Department of Cardiovascular Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors: Zhaoyun
authors: Z
authors: Cheng
authors: Department of Cardiovascular Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors: Deguang
authors: D
authors: Feng
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000009:adverse effects*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D004317:Doxorubicin / Q000009:adverse effects*; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; D047628:Estrogen Receptor alpha; D017382:Reactive Oxygen Species; D004317:Doxorubicin; D051057:Proto-Oncogene Proteins c-akt; C000617115:DDX17 protein, rat; D053487:DEAD-box RNA Helicases