title: Effects of Venous Superdrainage and Arterial Supercharging on Dorsal Perforator Flap in a Rat Model.
abstract: OBJECTIVE
  To comparatively assess the effects of venous superdrainage and arterial supercharging on dorsal perforator flap survival.
  
  
  METHODS
  Sixty male Sprague-Dawley rats (450-550g) were randomly divided into three groups (n = 20), including control group (Control) and experimental groups A (venous superdrainage, Exp. A) and B (arterial supercharging, Exp. B). At postoperative day 7, survival areas of the flaps were evaluated and all animals underwent angiography. Laser Doppler was used to evaluate flap perfusion from 0h to 7days after surgery. Histology with hematoxylin and eosin staining was used to count microvessels. Tissue of "Choke vessels"was excised for quantification of hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) by western blot assay at 6h and 7days after surgery.
  
  
  RESULTS
  In the Exp. A group, almost all flaps survived (98.2±1.6%); in the Exp. B and control group, survival areas accounted for 78.8±8.5% and 60.3±7.8%, respectively (P <0.001). In addition, Exp. A animals showed improved anastomosis of choke vessels 2 compared with the Exp. B and Control groups. Furthermore, flap blood flow and partial pressure of oxygen in the Exp. A group were significantly higher compared with values obtained for the Exp. B and Control groups, from 6 hours to 7 days after surgery. More microvessels were found in the Exp. A group (11.65±1.33) than in Exp. B (9.25±0.34) and control (7.25±0.91) animals on POD 7. The relative expression level of HIF-1α and VEGF were significant at 6h and 7days after surgery.
  
  
  CONCLUSIONS
  Venous superdrainage in rat dorsal perforator flap is more effective than arterial supercharging in promoting flap survival, and could effectively alter hemodynamics in the microcirculation and stimulate blood vessel formation.
authors: Department of human anatomy, Wenzhou Medical University, Wenzhou, China.
authors: Jun
authors: J
authors: Zheng
authors: Department of human anatomy, Wenzhou Medical University, Wenzhou, China.
authors: Shanshan
authors: S
authors: Xi
authors: Department of human anatomy, Wenzhou Medical University, Wenzhou, China.
authors: Maochao
authors: M
authors: Ding
authors: Department of human anatomy, Wenzhou Medical University, Wenzhou, China.
authors: Hong
authors: H
authors: Li
authors: Department of human anatomy, Wenzhou Medical University, Wenzhou, China.
authors: Wei
authors: W
authors: Xu
authors: Department of human anatomy, Wenzhou Medical University, Wenzhou, China.
authors: Maolin
authors: M
authors: Tang
authors: Department of human anatomy, Wenzhou Medical University, Wenzhou, China.
authors: Shixin
authors: S
authors: Chen
mesh_terms: D000818:Animals; D006439:Hemodynamics; D008297:Male; D008833:Microcirculation; D061525:Perforator Flap / Q000098:blood supply / Q000473:pathology / Q000637:transplantation*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016038:Skin Transplantation / Q000379:methods*