title: AK048794 maintains the mouse embryonic stem cell pluripotency by functioning as an miRNA sponge for miR-592.
abstract: MiR-592 has been identified as a neural-enriched microRNA, plays an important role in mNPCs differentiation, could induce astrogliogenesis differentiation arrest or/and enhance neurogenesis in vitro Previous studies showed that long noncoding RNAs (lncRNAs) were involved in the neuronal development and activity. To investigate the role of miR-592 in neurogenesis, we described the expression profile of lncRNAs in miR-592 knockout mouse embryonic stem cells (mESCs) and the corresponding normal mESCs by microarray. By the microarray analysis and luciferase reporter assays, we demonstrated that lncRNA - AK048794, regulated by transcription factor GATA1, functioned as a competing endogenous RNA (ceRNA) for miR-592 and led to the de-repression of its endogenous target FAM91A1, which is involved in mESC pluripotency maintenance. Taken together, these observations imply that AK048794 modulated the expression of multiple genes involved in mESC pluripotency maintenance by acting as a ceRNA for miR-592, which may build up the link between the regulatory miRNA network and mESC pluripotency.
authors: Tongji Hospital, School of Life Science and Technology, Tongji University, Shanghai 200065, PR China Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, PR China.
authors: Yang
authors: Y
authors: Zhou
authors: Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, PR China.
authors: Qing-Song
authors: QS
authors: Dai
authors: Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, PR China.
authors: Shi-Chang
authors: SC
authors: Zhu
authors: Tongji Hospital, School of Life Science and Technology, Tongji University, Shanghai 200065, PR China.
authors: Yue-Hua
authors: YH
authors: Han
authors: Tongji Hospital, School of Life Science and Technology, Tongji University, Shanghai 200065, PR China.
authors: Hai-Long
authors: HL
authors: Han
authors: Tongji Hospital, School of Life Science and Technology, Tongji University, Shanghai 200065, PR China.
authors: Bo
authors: B
authors: Zhao
authors: Tongji Hospital, School of Life Science and Technology, Tongji University, Shanghai 200065, PR China.
authors: Rong-Rong
authors: RR
authors: Gao
authors: Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, PR China.
authors: Jun
authors: J
authors: Zhang
authors: Tongji Hospital, School of Life Science and Technology, Tongji University, Shanghai 200065, PR China.
authors: Jing
authors: J
authors: Zhang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D000066450:Mouse Embryonic Stem Cells / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism*
chemical_list: C587924:MIRN592 microRNA, mouse; D035683:MicroRNAs; D062085:RNA, Long Noncoding
keywords: AK048794; GATA1; lncRNA; mESC; miR-592