title: Hydrogen-Rich Saline Attenuates Acute Hepatic Injury in Acute Necrotizing Pancreatitis by Inhibiting Inflammation and Apoptosis, Involving JNK and p38 Mitogen-Activated Protein Kinase-dependent Reactive Oxygen Species.
abstract: The objective of this study was to study the role of hydrogen-rich saline (HRS) on acute hepatic injury (AHI) in acute necrotizing pancreatitis (ANP).
  
  
  
  Rats were used for this study and an ANP model was induced by injecting 5% sodium taurocholate into the biliary-pancreatic duct. Experiments were performed in 3 groups: sham, ANP, and ANP + HRS (HRS). Animals were killed at 3, 12, and 24 hours after operation, and then blood and tissue samples were harvested. Various physiological, histological, and cellular and molecular parameters were analyzed.
  
  
  
  Analyses of serum, lipase, alanine transaminase, and aspartate aminotransferase indicated that ANP-induced AHI model was established successfully and HRS attenuated hepatic dysfunction. Hepatic superoxide dismutase and malondialdehyde levels showed HRS against oxidative stress. Cellular and molecular analyses including p-p38, p-JNK, p-ERK, and caspase-3, caspase-9, NF-κB, and TNF-α in hepatic tissues revealed that HRS attenuated ANP-induced AHI by inhibiting apoptosis and phosphorylation of JNK and p38, as well as NF-κB activation.
  
  
  
  Hydrogen-rich saline plays a protective role in ANP-induced AHI through inhibiting inflammation and apoptosis, involving JNK and p38 MAPK-dependent reactive oxygen species.
authors: From the *Department of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China; and †Department of Surgery, SUNY Upstate Medical University, Syracuse, NY.
authors: Qiao
authors: Q
authors: Shi
authors: Chen
authors: C
authors: Chen
authors: Wen-Hong
authors: WH
authors: Deng
authors: Peng
authors: P
authors: Wang
authors: Teng
authors: T
authors: Zuo
authors: Liang
authors: L
authors: Zhao
authors: Jia
authors: J
authors: Yu
authors: Kai-Liang
authors: KL
authors: Zhao
authors: Fang-Chao
authors: FC
authors: Mei
authors: Chen
authors: C
authors: Li
authors: Gui-Rong
authors: GR
authors: Wang
authors: Wei-Xing
authors: WX
authors: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D006859:Hydrogen; D007249:Inflammation; D019283:Pancreatitis, Acute Necrotizing; D051381:Rats; D017382:Reactive Oxygen Species; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D017382:Reactive Oxygen Species; D006859:Hydrogen; D048051:p38 Mitogen-Activated Protein Kinases