title: Fluorescent cyanine dyes for the quantification of low amounts of dsDNA.
abstract: In this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. The fluorescence intensity as a function of the amount of dsDNA is measured at the optimal wavelengths for excitation and emission and for each dye the limit of detection and the response linearity at low levels of dsDNA are determined. No linear range was found for SYBR Green and YOYO-1 for pg-ng quantities of dsDNA. EvaGreen, PicoGreen, AccuClear and AccuBlue NextGen show good linearity in the pg-ng range. AccuClear exhibits the widest linear range of 3 pg-200 ng, whereas AccuBlue NextGen turned out to have the highest sensitivity of the tested dyes with a limit of detection of 50 pg.
authors: Mesoscale Chemical Systems, MESA(+) Institute for Nanotechnology, University of Twente, Drienerlolaan 5, 7500 AE, Enschede, The Netherlands; Life Science, Life Sciences, Engineering & Design, Saxion University of Applied Sciences, M. H. Tromplaan 28, 7513 AB, Enschede, The Netherlands. Electronic address: b.b.bruijns@utwente.nl.
authors: B B
authors: BB
authors: Bruijns
authors: Life Science, Life Sciences, Engineering & Design, Saxion University of Applied Sciences, M. H. Tromplaan 28, 7513 AB, Enschede, The Netherlands.
authors: R M
authors: RM
authors: Tiggelaar
authors: Life Science, Life Sciences, Engineering & Design, Saxion University of Applied Sciences, M. H. Tromplaan 28, 7513 AB, Enschede, The Netherlands.
authors: J G E
authors: JG
authors: Gardeniers
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008297:Male; D012474:Salmon; D012680:Sensitivity and Specificity
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D004247:DNA
keywords: Absorbance; DNA detection; Fluorescence; dsDNA dyes