title: Fast and improved separation of major coumarins in Ammi visnaga (L.) Lam. by supercritical fluid chromatography.
abstract: The first supercritical fluid chromatography method for the determination of five major coumarins (dihydrosamidin, visnadin, samidin, khellin, and visnagin) in Ammi visnaga fruits is described. Their baseline separation was possible in less than 5 min by using a UPC2HSS C18SB column with 1.8 μm particle size and a mobile phase comprising CO2, methanol, acetonitrile, and diethylamine. The type of stationary phase used was of particular relevance because, except for the selected one, the others did not resolve the two structural isomers dihydrosamidin and visnadin. Method validation confirmed that the procedure is linear (R2≥ 0.9996) in a concentration range from 6 to 480 μg/mL, it is accurate (recovery rates: 97.2-103.6%) and precise (intraday deviation ≤ 6.6%, intraday deviation ≤ 1.7%); injecting 1 μL of standard solution, the determined limit of detection was below 1.9 μg/mL for all compounds. The analysis of different A. visnaga samples revealed their similar compositions, and khellin (0.75-1.01%) and visnagin (0.18-0.46%) were the dominant coumarins. Visnadin and dihydrosamidin, the individual quantification of which is described for the first time, were present at concentrations below 0.14%.
authors: Institute of Pharmacy, Pharmacognosy, Member of the CMBI, University of Innsbruck, Innsbruck, Austria.
authors: Bianca
authors: B
authors: Winderl
authors: Institute of Pharmacy, Pharmacognosy, Member of the CMBI, University of Innsbruck, Innsbruck, Austria.
authors: Stefan
authors: S
authors: Schwaiger
authors: Institute of Pharmacy, Pharmacognosy, Member of the CMBI, University of Innsbruck, Innsbruck, Austria. anzera@uibk.ac.at.
authors: Markus
authors: M
authors: Ganzera
mesh_terms: D031165:Ammi / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid; D003374:Coumarins / Q000302:isolation & purification*; D010936:Plant Extracts / Q000032:analysis*
chemical_list: D003374:Coumarins; D010936:Plant Extracts
keywords: Ammi visnaga; Coumarins; Supercritical fluid chromatography