title: Enhancement of cordyceps polysaccharide production via biosynthetic pathway analysis in Hirsutella sinensis.
abstract: The addition of various sulfates for enhanced cordyceps polysaccharide (CP) production in submerged cultivation of H. sinensis was investigated, and manganese sulfate was found the most effective. 2mM of manganese sulfate on 0day (d) was investigated as the optimal adding condition, and the CP production reached optimum with 5.33%, increasing by 93.3% compared with the control. Furthermore, the consumption of three main precursors of CP was studied over cultivation under two conditions. Intracellular mannose content decreased by 43.1% throughout 6days cultivation, which corresponded to CP accumulation rate sharply increased from 0 d to 6 d, and mannose was considered as the most preferred precursor for generating CP. Subsequently, mannose biosynthetic pathway was constructed and verified for the first time in H. sinensis, which constituted the important part of CP biosynthesis, and transcriptional levels of the biosynthetic genes were studied. Transcriptional level of gene cpsA was significantly up-regulated 5.35-fold and it was a key gene involved both in mannose and CP biosynthesis. This study demonstrated that manganese sulfate addition is an efficient and simple way to improve CP production. Transcriptional analysis based on biosynthetic pathway was helpful to find key genes and better understand CP biosynthesis.
authors: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
authors: Shan
authors: S
authors: Lin
authors: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
authors: Zhi-Qiang
authors: ZQ
authors: Liu
authors: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
authors: Peter James
authors: PJ
authors: Baker
authors: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
authors: Ming
authors: M
authors: Yi
authors: East China Pharmaceutical Group Limited Co., Ltd., Hangzhou 311000, China.
authors: Hui
authors: H
authors: Wu
authors: East China Pharmaceutical Group Limited Co., Ltd., Hangzhou 311000, China.
authors: Feng
authors: F
authors: Xu
authors: East China Pharmaceutical Group Limited Co., Ltd., Hangzhou 311000, China.
authors: Yi
authors: Y
authors: Teng
authors: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China. Electronic address: zhengyg@zjut.edu.cn.
authors: Yu-Guo
authors: YG
authors: Zheng
mesh_terms: D001203:Ascomycota / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018533:Biomass; D053898:Biosynthetic Pathways / Q000187:drug effects / Q000235:genetics; D002241:Carbohydrates / Q000032:analysis; D032661:Cordyceps / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005800:Genes, Fungal; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008358:Mannose / Q000096:biosynthesis; D008670:Metals / Q000494:pharmacology; D025282:Mycelium / Q000187:drug effects; D016366:Open Reading Frames / Q000235:genetics; D016133:Polymerase Chain Reaction; D011134:Polysaccharides / Q000096:biosynthesis*; D013431:Sulfates / Q000494:pharmacology; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D002241:Carbohydrates; D007477:Ions; D008670:Metals; D011134:Polysaccharides; D013431:Sulfates; D008358:Mannose
keywords: Biosynthetic pathway; Cordyceps polysaccharide; Hirsutella sinensis; Manganese sulfate; Transcriptional level