title: Effect of actinobacteria agent inoculation methods on cellulose degradation during composting based on redundancy analysis.
abstract: In this study, actinobacteria agent including Streptomyces sp. and Micromonospora sp. were inoculated during chicken manure composting by different inoculation methods. The effect of different treatments on cellulose degradation and the relationship between inoculants and indigenous actinobacteria were investigated during composting. The results showed that inoculation in different stages of composting all improved the actinobacteria community diversity particularly in the cooling stage of composting (M3). Moreover, inoculation could distinctly accelerate the degradation of organic matters (OM) especially celluloses. Redundancy analysis indicated that the correlation between indigenous actinobacteria and degradation of OM and cellulose were regulated by inoculants and there were significant differences between different inoculation methods. Furthermore, synergy between indigenous actinobacteria and inoculants for degradation of OM and cellulose in M3 was better than other treatments. Conclusively, we suggested an inoculation method to regulate the indigenous actinobacteria based on the relationship between inoculants and indigenous actinobacteria and degradation content.
authors: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors: Yue
authors: Y
authors: Zhao
authors: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors: Qian
authors: Q
authors: Lu
authors: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors: Yuquan
authors: Y
authors: Wei
authors: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors: Hongyang
authors: H
authors: Cui
authors: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors: Xu
authors: X
authors: Zhang
authors: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors: Xueqin
authors: X
authors: Wang
authors: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors: Si
authors: S
authors: Shan
authors: College of Life Science, Northeast Agricultural University, Harbin 150030, China. Electronic address: weizimin@neau.edu.cn.
authors: Zimin
authors: Z
authors: Wei
mesh_terms: D039903:Actinobacteria; D050260:Carbohydrate Metabolism; D002482:Cellulose; D008372:Manure / Q000382:microbiology*; D012037:Refuse Disposal / Q000379:methods*; D012987:Soil; D012988:Soil Microbiology
chemical_list: D008372:Manure; D012987:Soil; D002482:Cellulose
keywords: Cellulose degradation; Composting; Denaturing gradient gel electrophoresis (DGGE); Different stages inoculation; Redundancy analysis (RDA)