title: Anti-Inflammatory and Anti-Oxidant Effects of p-Chloro-phenyl-selenoesterol on TNBS-Induced Inflammatory Bowel Disease in Mice.
abstract: This study aims to investigate the protective effect of p-chloro-phenyl-selenoesterol [PCS; 0,2 mg/kg; 10 ml/kg i.g.) in colitis induced by 2,4,6-trinitrobenzene sulfonic acid [TNBS; 2 mg/100 µl 50% ethanol; intrarectally) in mice. Several parameters including weight, length, histological analyses determination, thiobarbituric acid reactive species, reactive species levels, superoxide dismutase, catalase, and myeloperoxidase (MPO) activity of colon were evaluated. The serum levels of tumor necrosis factor alpha [TNF-α) and interleukin 6 [IL-6) were also assessed. Treatment with PCS reduced the clinical and histopathologic severity of TNBS-induced colitis, characterized by colon length reduction and increased colon weight and microscopic intestinal inflammation. The therapeutic effects of PCS in this model were associated with significant decrease in proinflammatory cytokines TNF-α and IL-6 and decrease in MPO activity. Furthermore, combined with improvements in inflammatory parameters, treatment with the PCS was able to decrease oxidative stress and to prevent the decrease in antioxidant defenses in animals with TNBS-induced colitis. This finding suggests that PCS can improve experimental colitis in mice and it could be a potential therapeutic agent for the treatment of patients with IBD. J. Cell. Biochem. 118: 709-717, 2017. © 2016 Wiley Periodicals, Inc.
authors: Universidade Federal do Pampa, Campus Itaqui, CEP 97650-000, Rio Grande do Sul, Brazil.
authors: Micheli Stéfani
authors: MS
authors: Zarzecki
authors: Universidade Federal do Pampa, Campus Itaqui, CEP 97650-000, Rio Grande do Sul, Brazil.
authors: Vandreza Cardoso
authors: VC
authors: Bortolotto
authors: Universidade Federal do Pampa, Campus Itaqui, CEP 97650-000, Rio Grande do Sul, Brazil.
authors: Márcia Rósula
authors: MR
authors: Poetini
authors: Universidade Federal do Pampa, Campus Itaqui, CEP 97650-000, Rio Grande do Sul, Brazil.
authors: Stífani Machado
authors: SM
authors: Araujo
authors: Universidade Federal do Pampa, Campus Itaqui, CEP 97650-000, Rio Grande do Sul, Brazil.
authors: Mariane Trindade
authors: MT
authors: de Paula
authors: Universidade Regional Integrada, Campus Erechim, CEP 99700-000, Rio Grande do Sul, Brazil.
authors: Silvane Souza
authors: SS
authors: Roman
authors: Universidade Federal do Pampa, Campus Uruguaiana, CEP 97508-000, Rio Grande do Sul, Brazil.
authors: Cristiano
authors: C
authors: Spiazzi
authors: Universidade Federal do Pampa, Campus Uruguaiana, CEP 97508-000, Rio Grande do Sul, Brazil.
authors: Franciele Weber Santos
authors: FW
authors: Cibin
authors: Universidade Federal de Santa Maria, Campus Santa Maria, CEP 97105-900, Rio Grande do Sul, Brazil.
authors: Oscar Endrigo Dorneles
authors: OE
authors: Rodrigues
authors: Universidade Federal do Pampa, Campus Itaqui, CEP 97650-000, Rio Grande do Sul, Brazil.
authors: Cristiano Ricardo
authors: CR
authors: Jesse
authors: Universidade Federal do Pampa, Campus Itaqui, CEP 97650-000, Rio Grande do Sul, Brazil.
authors: Marina
authors: M
authors: Prigol
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D016566:Organoselenium Compounds / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016207:Cytokines; D018836:Inflammation Mediators; D016566:Organoselenium Compounds; C000624381:p-chloro-phenyl-selenoesterol; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase
keywords: IBD; INFLAMMATION; KEYWORDS; OXIDATIVE STRESS; SELENIUM