title: The expression of thioredoxin-1 in acute epinephrine stressed mice.
abstract: Stress, a state of perceived threat to homeostasis, regulates a panel of important physiological functions. The human mind and body respond to stress by activating the sympathetic nervous system and secreting the catecholamines epinephrine and norepinephrine in the "fight-or-flight" response. However, the protective mechanism of acute stress is still unknown. In the present study, an acute stress mouse model was constructed by intraperitoneal injection of epinephrine (0.2 mg kg(-1)) for 4 h. Epinephrine treatment induced heat shock 70(Hsp70) expression in the stress responsive tissues, such as the cortex, hippocampus, thymus, and kidney. Further, the expression of thioredoxin-1(Trx-1), a cytoprotective protein, was also upregulated in these stress responsive tissues. In addition, the phosphorylation of cAMP-response element binding protein (CREB), a transcription factor of Trx-1, was increased after treatment with epinephrine. The block of CREB activation by H89 inhibited the acute epinephrine stress-induced Trx-1 and Hsp70 expression. Taken together, our data suggest that acute stimuli of epinephrine induced Trx-1 expression through activating CREB and may represent a protective role against stress.
authors: College of Life Sciences, Xinyang Normal University, Xinyang, 464000, China.|Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, 464000, China.
authors: Jin-Jing
authors: JJ
authors: Jia
authors: College of Life Sciences, Xinyang Normal University, Xinyang, 464000, China. xszeng@xynu.edu.cn.
authors: Xian-Si
authors: XS
authors: Zeng
authors: College of Life Sciences, Xinyang Normal University, Xinyang, 464000, China.
authors: Kun
authors: K
authors: Li
authors: College of Life Sciences, Xinyang Normal University, Xinyang, 464000, China.
authors: Li-Fang
authors: LF
authors: Ma
authors: College of Life Sciences, Xinyang Normal University, Xinyang, 464000, China.
authors: Lei
authors: L
authors: Chen
authors: College of Life Sciences, Xinyang Normal University, Xinyang, 464000, China.
authors: Xin-Qiang
authors: XQ
authors: Song
mesh_terms: D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology / Q000502:physiology*; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D016716:PC12 Cells; D051381:Rats; D013315:Stress, Psychological / Q000378:metabolism; D013879:Thioredoxins / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: C492004:Creb1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D018840:HSP70 Heat-Shock Proteins; C515559:Txn1 protein, mouse; D013879:Thioredoxins; D017868:Cyclic AMP-Dependent Protein Kinases; D004837:Epinephrine
keywords: Acute stress; CREB; Epinephrine; Hsp70; Trx-1