title: Hydroxyapatite (HA) microparticles labeled with (32)P - A promising option in the radiation synovectomy for inflamed joints.
abstract: In the present article we describe a systematic approach pursued for the synthesis of (32)P-labeled hydroxyapatite (HA) microparticles (1-10µm size range) using no carrier added (NCA) (32)P produced in a nuclear reactor and animal evaluation of its utility as an expected viable radiopharmaceutical for the treatment of pain intensive arthrosis. NCA (32)P was produced via the (32)S(n,p)(32)P route in nuclear reactor with high radionuclidic purity (99.95±0.01%, n=5). Phosphorus-32-labeled hydroxyapatite microparticles (1-10µm size range) were synthesized with high radiochemical purity (99.0±0.3% n=12) under optimized conditions and the formulation showed excellent in vitro stability in saline as well as in rat serum. Intra-articular administration of the radiolabeled particles in the knee joints of normal Wistar rats showed near-complete retention of activity within the synovial cavity upto 1 month post-administration. The radiochemical formulation thus demonstrated promising features as a radiopharmaceutical for treatment of arthritis with excellent logistic advantage for shipment to sites distant from the production facility thanks to the suitable nuclear decay properties of (32)P.
authors: Isotope Production and Applications Division, Bhabha Atomic Research Centre (BARC), Mumbai, 400085 India.
authors: A
authors: A
authors: Rajeswari
authors: Isotope Production and Applications Division, Bhabha Atomic Research Centre (BARC), Mumbai, 400085 India.
authors: K V
authors: KV
authors: Vimalnath
authors: Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre (BARC), Mumbai, 400085 India.
authors: H D
authors: HD
authors: Sarma
authors: Isotope Production and Applications Division, Bhabha Atomic Research Centre (BARC), Mumbai, 400085 India.
authors: Priyalata
authors: P
authors: Shetty
authors: Isotope Production and Applications Division, Bhabha Atomic Research Centre (BARC), Mumbai, 400085 India.
authors: Shahiralm Khan
authors: SK
authors: Mohammed
authors: Chemistry Division, Bhabha Atomic Research Centre (BARC), Mumbai, 400085 India.
authors: Jitendra
authors: J
authors: Nuwad
authors: Isotope Production and Applications Division, Bhabha Atomic Research Centre (BARC), Mumbai, 400085 India. Electronic address: sudipta@barc.gov.in.
authors: Sudipta
authors: S
authors: Chakraborty
authors: Isotope Production and Applications Division, Bhabha Atomic Research Centre (BARC), Mumbai, 400085 India.
authors: Ashutosh
authors: A
authors: Dash
mesh_terms: D000818:Animals; D001168:Arthritis / Q000473:pathology* / Q000532:radiotherapy*; D002214:Capsules / Q000008:administration & dosage* / Q000138:chemical synthesis*; D017886:Durapatite / Q000737:chemistry*; D005240:Feasibility Studies; D007553:Isotope Labeling / Q000379:methods; D009928:Organ Specificity; D010761:Phosphorus Radioisotopes / Q000737:chemistry / Q000627:therapeutic use*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D016896:Treatment Outcome
chemical_list: D002214:Capsules; D010761:Phosphorus Radioisotopes; D019275:Radiopharmaceuticals; D017886:Durapatite
keywords: Arthritis; Hydroxyapatite (HA) microparticles; NCA (32)P; Radiation synovectomy; Radiolabeling