title: Towards the development of a sustainable soya bean-based feedstock for aquaculture.
abstract: Soya bean (Glycine max (L.) Merr.) is sought after for both its oil and protein components. Genetic approaches to add value to either component are ongoing efforts in soya bean breeding and molecular biology programmes. The former is the primary vegetable oil consumed in the world. Hence, its primary usage is in direct human consumption. As a means to increase its utility in feed applications, thereby expanding the market of soya bean coproducts, we investigated the simultaneous displacement of marine ingredients in aquafeeds with soya bean-based protein and a high Omega-3 fatty acid soya bean oil, enriched with alpha-linolenic and stearidonic acids, in both steelhead trout (Oncorhynchus mykiss) and Kampachi (Seriola rivoliana). Communicated herein are aquafeed formulations with major reduction in marine ingredients that translates to more total Omega-3 fatty acids in harvested flesh. Building off of these findings, subsequent efforts were directed towards a genetic strategy that would translate to a prototype design of an optimal identity-preserved soya bean-based feedstock for aquaculture, whereby a multigene stack approach for the targeted synthesis of two value-added output traits, eicosapentaenoic acid and the ketocarotenoid, astaxanthin, were introduced into the crop. To this end, the systematic introduction of seven transgenic cassettes into soya bean, and the molecular and phenotypic evaluation of the derived novel events are described.
authors: Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA.|Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors: Hyunwoo
authors: H
authors: Park
authors: Department of Food Science and Technology, The Food Processing Center, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors: Steven
authors: S
authors: Weier
authors: Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors: Fareha
authors: F
authors: Razvi
authors: Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA.|Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors: Pamela A
authors: PA
authors: Peña
authors: Kampachi Farms, LLC, Kona, HI, USA.
authors: Neil A
authors: NA
authors: Sims
authors: Kampachi Farms, LLC, Kona, HI, USA.
authors: Jennica
authors: J
authors: Lowell
authors: Kampachi Farms, LLC, Kona, HI, USA.
authors: Cory
authors: C
authors: Hungate
authors: Kampachi Farms, LLC, Kona, HI, USA.
authors: Karma
authors: K
authors: Kissinger
authors: Kampachi Farms, LLC, Kona, HI, USA.
authors: Gavin
authors: G
authors: Key
authors: Centre for Systems and Synthetic Biology, School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, UK.
authors: Paul
authors: P
authors: Fraser
authors: Department of Biological Chemistry, Rothamsted Research, Harpenden, Herts, UK.
authors: Johnathan A
authors: JA
authors: Napier
authors: Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.|Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors: Edgar B
authors: EB
authors: Cahoon
authors: Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA.|Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors: Tom E
authors: TE
authors: Clemente
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D005399:Fishes / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000378:metabolism; D010938:Plant Oils; D030821:Plants, Genetically Modified; D013024:Soybean Oil / Q000008:administration & dosage; D013025:Glycine max / Q000235:genetics / Q000254:growth & development*; D024341:Xanthophylls / Q000378:metabolism; D017962:alpha-Linolenic Acid
chemical_list: D015525:Fatty Acids, Omega-3; D010938:Plant Oils; D024341:Xanthophylls; D017962:alpha-Linolenic Acid; D013024:Soybean Oil; C005948:astaxanthine; D015118:Eicosapentaenoic Acid; C062895:stearidonic acid
keywords: 
  Glycine max
  ; astaxanthin; eicosapentaenoic acid; long-chain Omega-3 fatty acids; soya bean oil