title: Methods to Study Interactions Between Ciliogenesis and Autophagy.
abstract: Autophagy is a catabolic pathway for the degradation and recycling of intracellular components, contributing to maintain cell homeostasis. Changes in autophagy activity can be monitored by a variety of biochemical and functional assays that should be used in combination. Recently, it has been described that signaling from the primary cilium modulates autophagy. This novel and reciprocal interaction will impact diverse aspects of the cell biology in healthy and pathophysiological conditions. Here, we describe methods to monitor autophagy activity in cilia mutants, as well as the use of autophagy mutants to monitor ciliogenesis.
authors: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, 10461, USA.
authors: Birgit Hegner
authors: BH
authors: Satir
authors: Institut des Maladies Neurodégénératives, UMR 5293, Université de Bordeaux, Bât 3B 1er étage, 146 rue Léo Saignat, 33076, Bordeaux Cedex, France. olatz.pampliega@u-bordeaux.fr.|CNRS, Institut des Maladies Neurodégénératives, UMR 5293, 33000, Bordeaux Cedex, France. olatz.pampliega@u-bordeaux.fr.
authors: Olatz
authors: O
authors: Pampliega
mesh_terms: D000818:Animals; D000071182:Autophagosomes / Q000378:metabolism; D001343:Autophagy; D002923:Cilia / Q000378:metabolism*; D005347:Fibroblasts; D015870:Gene Expression; D055786:Gene Knockout Techniques; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D038081:Organogenesis
chemical_list: D049452:Green Fluorescent Proteins
keywords: Autophagy; Immunofluorescence; LC3-II; Primary cilia; Western blotting; mCherry-GFP-LC3