title: Crystal structure of Arabidopsis thaliana calmodulin7 and insight into its mode of DNA binding.
abstract: Calmodulin (CaM) is a Ca(2+) sensor that participates in several cellular signaling cascades by interacting with various targets, including DNA. It has been shown that Arabidopsis thaliana CaM7 (AtCaM7) interacts with Z-box DNA and functions as a transcription factor [Kushwaha R et al. (2008) Plant Cell 20, 1747-1759; Abbas N et al. (2014) Plant Cell 26, 1036-1052]. The crystal structure of AtCaM7, and a model of the AtCAM7-Z-box complex suggest that Arg-127 determines the DNA-binding ability by forming crucial interactions with the guanine base. We validated the model using biolayer interferometry, which confirmed that AtCaM7 interacts with Z-box DNA with high affinity. In contrast, the AtCaM2/3/5 isoform does not show any binding, although it differs from AtCaM7 by only a single residue.
authors: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
authors: Sanjeev
authors: S
authors: Kumar
authors: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
authors: Mohit
authors: M
authors: Mazumder
authors: Department of Biotechnology, National Institute of Technology, Durgapur, India.
authors: Nisha
authors: N
authors: Gupta
authors: Department of Biotechnology, National Institute of Technology, Durgapur, India.
authors: Sudip
authors: S
authors: Chattopadhyay
authors: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
authors: Samudrala
authors: S
authors: Gourinath
mesh_terms: D017360:Arabidopsis / Q000737:chemistry; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002147:Calmodulin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D011485:Protein Binding / Q000235:genetics; D011487:Protein Conformation
chemical_list: D029681:Arabidopsis Proteins; C465131:CaM7 protein, Arabidopsis; D002147:Calmodulin; D004268:DNA-Binding Proteins; D004247:DNA
keywords: CaM; molecular modeling; protein crystallization; protein-DNA interaction