title: A novel nonenzymatic biosensor for evaluation of oxidative stress based on nanocomposites of graphene blended with CuI.
abstract: A high-sensitive nonenzymatic hydrogen peroxide (H2O2) biosensor based on cuprous iodide and graphene (CuI/Gr) composites has been explored for the detection of H2O2 released by living cells and monitoring the oxidative stress of cells under excellular stimulation. The biosensor properties were evaluated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), amperometric i-t curve, and the redox-competition mode of scanning electrochemical microscopy (SECM). Our observations demonstrate that the CuI/Gr nanocomposites modified glassy carbon electrode (GCE) exhibits excellent catalytic activity for H2O2 with relatively low detection limit and a wide linear range from 0.5 μM to 3 mM. Moreover, the redox-competition mode of SECM imaging study further illustrates the improved electrochemical catalytic capability for H2O2 reduction with CuI/Gr nanocomposites deposited on graphite electrode. Hence, the as-prepared nonenzymatic H2O2 biosensor could be used to detect H2O2 release from different kinds of living cells under stimulation while eliminating the interference of ascorbic acid.
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China; School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
authors: Changhui
authors: C
authors: Li
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
authors: Xiaoli
authors: X
authors: Liu
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
authors: Yuanyuan
authors: Y
authors: Zhang
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
authors: Yun
authors: Y
authors: Chen
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
authors: Tianyu
authors: T
authors: Du
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
authors: Hui
authors: H
authors: Jiang
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address: xuewang@seu.edu.cn.
authors: Xuemei
authors: X
authors: Wang
mesh_terms: D015374:Biosensing Techniques; D003300:Copper / Q000737:chemistry*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D007454:Iodides / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D018384:Oxidative Stress; D014407:Tumor Cells, Cultured
chemical_list: D007454:Iodides; D006108:Graphite; D003300:Copper; C073870:cuprous iodide; D006861:Hydrogen Peroxide
keywords: Cells; Cuprous iodide; Graphene; Hydrogen peroxide; Oxidative stress