title: Bead-beating artefacts in the Bacteroidetes to Firmicutes ratio of the human stool metagenome.
abstract: We evaluated bead-beating cell-lysis in analysing the human stool metagenome, since this is a key step. We observed that two different bead-beating instruments from the same producer gave a three-fold difference in the Bacteroidetes to Firmicutes ratio. This illustrates that bead-beating can have a major impact on downstream metagenome analyses.
authors: Genetic Analysis AS, Oslo, Norway.
authors: Heidi C
authors: HC
authors: Vebø
authors: Genetic Analysis AS, Oslo, Norway.
authors: Magdalena Kauczynska
authors: MK
authors: Karlsson
authors: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway.
authors: Ekaterina
authors: E
authors: Avershina
authors: Genetic Analysis AS, Oslo, Norway.
authors: Lene
authors: L
authors: Finnby
authors: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway. Electronic address: knut.rudi@nmbu.no.
authors: Knut
authors: K
authors: Rudi
mesh_terms: D016477:Artifacts; D041963:Bacteroidetes / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D000068536:Bacillota / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D054892:Metagenome; D008863:Microspheres; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D013048:Specimen Handling / Q000379:methods
chemical_list: D004269:DNA, Bacterial
keywords: Bead-beating; Human; Metagenome