title: Toxicity analysis of ocular prosthesis acrylic resin with or without pigment incorporation in human conjunctival cell line.
abstract: The aim of this study was to evaluate the influence of pigment incorporation on the cytotoxicity of ocular prosthesis N1 color acrylic resin. Nine samples were manufactured by heat-polymerization in water bath and divided into 3 groups: acrylic resin without pigment incorporation (group R), acrylic resin with pigment incorporation (group RP), and acrylic pigment (group P). Eluates formed after 72h of sample immersion in medium were incubated with conjunctival cell line (Chang conjunctival cells) for 72h. The negative control group consisted in medium without samples (group C). The cytotoxic effect from the eluates was evaluated using MTT assay (cell proliferation), ELISA assay (quantification of IL1β, IL6, TNF α and CCL3/MIP1α) and RT-PCR assay (mRNA expression of COL IV, TGF β and MMP9). Data were submitted to ANOVA with Bonferroni post-tests (p<0.05). All groups were considered non-cytotoxic based on cell proliferation. However, resin with pigment incorporation showed significant IL6 quantity increase. Resin without pigment incorporation exhibited higher mRNA expression of COL IV, MMP9 and TGF β, however it was also observed for the negative control group. The materials exhibited divergent biological behavior. Despite the pigment incorporation that resulted in an increase of IL6, no cytotoxicity was observed based on cell proliferation.
authors: Department of Dental Materials and Prosthodontics, Aracatuba Dental School, Sao Paulo State University (UNESP), Jose Bonifacio St., 1153, Vila Mendonca, Aracatuba, Sao Paulo, Brazil.
authors: Emily Vivianne Freitas
authors: EVF
authors: da Silva
authors: Department of Dental Materials and Prosthodontics, Aracatuba Dental School, Sao Paulo State University (UNESP), Jose Bonifacio St., 1153, Vila Mendonca, Aracatuba, Sao Paulo, Brazil. Electronic address: goiato@foa.unesp.br.
authors: Marcelo Coelho
authors: MC
authors: Goiato
authors: Department of Dental Materials and Prosthodontics, Aracatuba Dental School, Sao Paulo State University (UNESP), Jose Bonifacio St., 1153, Vila Mendonca, Aracatuba, Sao Paulo, Brazil.
authors: Liliane da Rocha
authors: LDR
authors: Bonatto
authors: Department of Dental Materials and Prosthodontics, Aracatuba Dental School, Sao Paulo State University (UNESP), Jose Bonifacio St., 1153, Vila Mendonca, Aracatuba, Sao Paulo, Brazil.
authors: Rodrigo Antonio
authors: RA
authors: de Medeiros
authors: Department of Dental Materials and Prosthodontics, Aracatuba Dental School, Sao Paulo State University (UNESP), Jose Bonifacio St., 1153, Vila Mendonca, Aracatuba, Sao Paulo, Brazil.
authors: Daniela Micheline Dos
authors: DMD
authors: Santos
authors: Department of Basic Sciences, Aracatuba Dental School, Sao Paulo State University (UNESP), Jose Bonifacio St., 1153, Vila Mendonca, Aracatuba, Sao Paulo, Brazil.
authors: Elidiane Cipriano
authors: EC
authors: Rangel
authors: Technological Plasma Laboratory (LaPTec), Experimental Campus of Sorocaba, UNESP, 3 de Marco Av., Alto da Boa Vista, Sorocaba, Sao Paulo, Brazil.
authors: Sandra Helena Penha de
authors: SHP
authors: Oliveira
mesh_terms: D000180:Acrylic Resins / Q000633:toxicity*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D024141:Collagen Type IV / Q000235:genetics; D004396:Coloring Agents / Q000633:toxicity*; D003228:Conjunctiva / Q000166:cytology*; D005137:Eye, Artificial; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics
chemical_list: D000180:Acrylic Resins; D024141:Collagen Type IV; D004396:Coloring Agents; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Acrylic resins; Biocompatibility testing; Dental material; Monomers; Ocular prosthesis