title: Globin mRNA reduction for whole-blood transcriptome sequencing.
abstract: The transcriptome analysis of whole-blood RNA by sequencing holds promise for the identification and tracking of biomarkers; however, the high globin mRNA (gmRNA) content of erythrocytes hampers whole-blood and buffy coat analyses. We introduce a novel gmRNA locking assay (GlobinLock, GL) as a robust and simple gmRNA reduction tool to preserve RNA quality, save time and cost. GL consists of a pair of gmRNA-specific oligonucleotides in RNA initial denaturation buffer that is effective immediately after RNA denaturation and adds only ten minutes of incubation to the whole cDNA synthesis procedure when compared to non-blood RNA analysis. We show that GL is fully effective not only for human samples but also for mouse and rat, and so far incompletely studied cow, dog and zebrafish.
authors: Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.|Competence Centre on Health Technologies, Tartu, Estonia.|Molecular Neurology Research Program, University of Helsinki and Folkhälsan Institute of Genetics, Helsinki, Finland.
authors: Kaarel
authors: K
authors: Krjutškov
authors: Competence Centre on Health Technologies, Tartu, Estonia.|Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
authors: Mariann
authors: M
authors: Koel
authors: Competence Centre on Health Technologies, Tartu, Estonia.
authors: Anne Mari
authors: AM
authors: Roost
authors: Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.
authors: Shintaro
authors: S
authors: Katayama
authors: Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.|Molecular Neurology Research Program, University of Helsinki and Folkhälsan Institute of Genetics, Helsinki, Finland.
authors: Elisabet
authors: E
authors: Einarsdottir
authors: Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.
authors: Eeva-Mari
authors: EM
authors: Jouhilahti
authors: Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.|Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden.
authors: Cilla
authors: C
authors: Söderhäll
authors: Competence Centre on Health Technologies, Tartu, Estonia.|Department of Reproductive Biology, Estonian University of Life Sciences, Tartu, Estonia.
authors: Ülle
authors: Ü
authors: Jaakma
authors: Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
authors: Mario
authors: M
authors: Plaas
authors: Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.
authors: Liselotte
authors: L
authors: Vesterlund
authors: Molecular Neurology Research Program, University of Helsinki and Folkhälsan Institute of Genetics, Helsinki, Finland.
authors: Hannes
authors: H
authors: Lohi
authors: Competence Centre on Health Technologies, Tartu, Estonia.|Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.|Department of Obstetrics and Gynaecology, University of Tartu, Tartu, Estonia.|Department of Obstetrics and Gynecology, Helsinki University Hospital, Helsinki, Finland.
authors: Andres
authors: A
authors: Salumets
authors: Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.|Molecular Neurology Research Program, University of Helsinki and Folkhälsan Institute of Genetics, Helsinki, Finland.
authors: Juha
authors: J
authors: Kere
mesh_terms: D001773:Blood Cells / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D005914:Globins / Q000737:chemistry*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000097:blood / Q000737:chemistry*; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger; D005914:Globins