title: Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab')2, and Streptavidin.
abstract: Colloidal gold probes, including protein A/G/L, IgG, F(ab')2, and streptavidin labeled with gold particles, are useful tools to localize antigens in cells and tissues by immunoelectron microscopy (IEM). This chapter describes different methods for the preparation of colloidal gold and conjugation of colloidal gold to protein A/G/L, IgG, and streptavidin.
authors: Section of Functional Morphology, Faculty of Pharmaceutical Sciences, Nagasaki International University, Hius Ten Bosch 2825-7, Sasebo, Nagasaki, 859-3298, Japan. syokota123@gmail.com.
authors: Sadaki
authors: S
authors: Yokota
mesh_terms: D000941:Antigens / Q000032:analysis; D001426:Bacterial Proteins / Q000737:chemistry* / Q000276:immunology; D002460:Cell Line; D017979:Gold Colloid / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D016253:Microscopy, Immunoelectron; D013205:Staphylococcal Protein A / Q000737:chemistry* / Q000276:immunology; D019809:Streptavidin / Q000737:chemistry
chemical_list: D000941:Antigens; D001426:Bacterial Proteins; D017979:Gold Colloid; C055047:Ig L-binding protein, Peptostreptococcus; C034284:IgG Fc-binding protein, Streptococcus; D018796:Immunoconjugates; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D019809:Streptavidin
keywords: IgG–gold; Preparation of colloidal gold; Protein A/G/L–gold; Sizing of gold particles; Streptavidin–gold