title: Human cytomegalovirus prevalence and distribution of glycoprotein B, O genotypes among hospitalized children with respiratory infections in West China, 2009-2014.
abstract: OBJECTIVE
  Human cytomegalovirus (HCMV) is an important pathogen causing morbidity and mortality in children. HCMV prevalence in children with respiratory infections has not been investigated in West China. Previous studies have suggested that glycoproteins genotypes may be associated with different clinical presentations, but the associations were controversial. The aim of this study was to determine the prevalence of HCMV infection in children with respiratory infections, the distributions of gB, gO genotypes among these isolates and their potential predictive roles for the development of symptoms in children.
  
  
  METHODS
  A total of 1709 respiratory specimens were obtained from hospitalised children with respiratory symptoms from 2009 to 2014 for the confirmation of HCMV infection. Glycoprotein B,O genotyping was carried out by multiplex nested PCR and sequencing.
  
  
  RESULTS
  The overall infection rate was 10.8%, and dominant genotypes were gB1 (74.2%) and gO1 (37.1%). Clinical characteristics differed between infants and children >1 year of age. Infants infected with HCMV had a higher frequency of fever (P < 0.001), cough (P < 0.001), rhinorrhea (P < 0.001), expectoration (P = 0.001) and diarrhoea (P = 0.005). Children <1 year age infected with gB1 had a higher rate of cough (P = 0.0192).
  
  
  CONCLUSIONS
  Infants infected with HCMV had a severe clinical outcome. gB1 may negatively associate with clinical presentations and quality of life in these children. The prevalence of HCMV infection and genotype distribution emphasises the importance of HCMV screening, vaccination and control for transmission.
authors: Department of Public Health Laboratory Sciences, West China School of Public Health, Sichuan University, Chengdu, China.
authors: Jia-Yi
authors: JY
authors: Chen
authors: Department of Public Health Laboratory Sciences, West China School of Public Health, Sichuan University, Chengdu, China.
authors: Tian-Li
authors: TL
authors: Zheng
authors: Department of Public Health Laboratory Sciences, West China School of Public Health, Sichuan University, Chengdu, China.
authors: Tao
authors: T
authors: Zhou
authors: Department of Public Health Laboratory Sciences, West China School of Public Health, Sichuan University, Chengdu, China.|Shenzhen Nanshan Center for Disease Control and Prevention, Shenzhen, China.
authors: Peng-Wei
authors: PW
authors: Hu
authors: Department of Public Health Laboratory Sciences, West China School of Public Health, Sichuan University, Chengdu, China.
authors: Meng-Jiao
authors: MJ
authors: Huang
authors: Department of Public Health Laboratory Sciences, West China School of Public Health, Sichuan University, Chengdu, China.
authors: Xin
authors: X
authors: Xu
authors: Department of Public Health Laboratory Sciences, West China School of Public Health, Sichuan University, Chengdu, China. xxpeiscu@163.com.
authors: Xiao-Fang
authors: XF
authors: Pei
mesh_terms: D000293:Adolescent; D002648:Child; D002673:Child, Hospitalized; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D003586:Cytomegalovirus Infections / Q000453:epidemiology / Q000235:genetics* / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012621:Seasons
chemical_list: D004279:DNA, Viral; D006023:Glycoproteins
keywords: Citomegalovirus humano; Epidemiología Molecular; Genotipos; Población Pediátrica; Presentación clínica; clinical presentations; cytomégalovirus humain; genotypes; génotypes; human cytomegalovirus; molecular epidemiology; paediatric population; population pédiatrique; présentations cliniques; épidémiologie moléculaire