title: An ion extract obtained from mineral trioxide aggregate induced dentin remineralization and dentin tubule occlusion in artificially demineralized bovine dentin.
abstract: OBJECTIVE
  To investigate the ability of a mineral trioxide aggregate (MTA) extract mixed with a phosphate buffered saline (PBS) system to induce remineralization and dentin tubule occlusion in artificially demineralized bovine dentin.
  
  
  METHODS
  The MTA extract solution was prepared by mixing white ProRoot MTA with distilled water (1:2) for 48 hours, before subjecting it to centrifugation. The elemental composition of the MTA extract solution was analyzed with inductively coupled plasma atomic emission spectrometry. The deposits produced by the MTA extract-PBS mixture were chemically analyzed using electron probe microanalysis (EPMA) and X-ray diffraction (XRD). The effects of the two-step application of the mixture (MTA extract solution followed by PBS) to bovine dentin samples that had been artificially demineralized with phosphoric acid (10%, 10 seconds) were investigated with scanning electron microscopy and EPMA after the specimens had been stored in PBS for 1 or 7 days.
  
  
  RESULTS
  The MTA extract solution contained calcium, silicone, and aluminum (Ca>Si>Al), and the deposits produced by the MTA extract-PBS mixture contained calcium, phosphorous, sodium, silicone, and aluminum (Ca>P>Na>Si>Al) as major mineral elements. XRD also revealed that the deposits contained hydroxyapatite. The two-step application process resulted in the formation of a 2-3 microm-thick "mineral infiltration layer", together with mineral tag-like structures in the dentin tubules. The MTA extract-treated specimens exhibited a significantly higher dentin tubule occlusion rate than the untreated specimens (P < 0.05).
authors: Linlin
authors: L
authors: Han
authors: Takashi
authors: T
authors: Okiji
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D003804:Dentin / Q000187:drug effects*; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology*; D012996:Solutions; D014089:Tooth Remineralization; D014961:X-Ray Diffraction
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D017640:Silicates; D012996:Solutions; C086631:mineral trioxide aggregate