title: Development of an ELISA using anti-idiotypic antibody for diagnosis of opisthorchiasis.
abstract: Monoclonal antibody specific for an epitope of cretory-secretory antigen protein of Opisthorchis felineus (Rivolta, 1884) (Trematoda: Opisthorchiidae) with a molecular weight of 28 kDa was used in a sandwich enzyme-linked immunosorbent assay (ELISA) for immobilisation of liver fluke specific antigen to the solid phase. Examination of human sera by this ELISA compared with commercial assays demonstrated that the monoclonal antibody epitope is located within this significant parasite protein. Anti-idiotypic antibody specific for the paratope of this monoclonal antibody was obtained by a hybridoma technique. Mimicking an epitope of excretory-secretory antigen of O. felineus, it had the capacity to bind specific antibody and elicit an antibody response. The value of anti-idiotypic antibody as a substitute for the liver fluke antigen was tested by ELISA using serum samples of infected dogs. Anti-idiotypic antibody proved to be of value in both an indirect-ELISA and a competitive-ELISA for diagnosis of opisthorchiasis. Mature trematodes were isolated from all infected animals. The faecal egg counts were negative in dogs with a relatively small number of parasites, despite finding antibodies in serum by ELISA. Substitution of parasite antigen with anti-idiotype avoids the use of experimental animals and also reduces time-consuming steps of antigen preparation.
authors: Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agro-Technical University, Astana, Kazakhstan;
authors: Aitbay K
authors: AK
authors: Bulashev
authors: Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agro-Technical University, Astana, Kazakhstan;
authors: Sergey N
authors: SN
authors: Borovikov
authors: Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agro-Technical University, Astana, Kazakhstan;
authors: Shynar S
authors: SS
authors: Serikova
authors: Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agro-Technical University, Astana, Kazakhstan;
authors: Zhanbolat A
authors: ZA
authors: Suranshiev
authors: Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agro-Technical University, Astana, Kazakhstan;
authors: Vladimir S
authors: VS
authors: Kiyan
authors: Cell Engineering Laboratory, National Centre for Biotechnology of the Republic of Kazakhstan, Astana, Kazakhstan.
authors: Saule Z
authors: SZ
authors: Eskendirova
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009889:Opisthorchiasis / Q000097:blood* / Q000175:diagnosis; D009891:Opisthorchis; D012680:Sensitivity and Specificity
chemical_list: D000888:Antibodies, Anti-Idiotypic
keywords: Opisthorchis felineus; antigenicity; excretory-secretory antigen; immunogenicity; monoclonal antibody; polyclonal antibody