title: Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes.
abstract: Due to their excellent physical and chemical characteristics, multi-wall carbon nanotubes (MWCNT) have the potential to be used in structural composites, conductive materials, sensors, drug delivery and medical imaging. However, because of their small-size and light-weight, the applications of MWCNT also raise health concerns. In vivo animal studies have shown that MWCNT cause biomechanical and genetic alterations in the lung tissue which lead to lung fibrosis. To screen the fibrogenic risk factor of specific types of MWCNT, we developed a human lung microtissue array device that allows real-time and in-situ readout of the biomechanical properties of the engineered lung microtissue upon MWCNT insult. We showed that the higher the MWCNT concentration, the more severe cytotoxicity was observed. More importantly, short type MWCNT at low concentration of 50 ng/ml stimulated microtissue formation and contraction force generation, and caused substantial increase in the fibrogenic marker miR-21 expression, indicating the high fibrogenic potential of this specific carbon nanotube type and concentration. The presented microtissue array system provides a powerful tool for high-throughput examination of the therapeutic and toxicological effects of target compounds in realistic tissue environment.
authors: State University of New York at Buffalo, Department of Biomedical Engineering, Buffalo, New York, 14260, USA.
authors: Zhaowei
authors: Z
authors: Chen
authors: State University of New York at Buffalo, Department of Biomedical Engineering, Buffalo, New York, 14260, USA.
authors: Qixin
authors: Q
authors: Wang
authors: State University of New York at Buffalo, Department of Biomedical Engineering, Buffalo, New York, 14260, USA.
authors: Mohammadnabi
authors: M
authors: Asmani
authors: State University of New York at Buffalo, Department of Biomedical Engineering, Buffalo, New York, 14260, USA.
authors: Yan
authors: Y
authors: Li
authors: State University of New York at Buffalo, Department of Biomedical Engineering, Buffalo, New York, 14260, USA.
authors: Chang
authors: C
authors: Liu
authors: State University of New York at Buffalo, Department of Biomedical Engineering, Buffalo, New York, 14260, USA.|State University of New York at Buffalo, Department of Chemical and Biological Engineering, Buffalo, New York, 14260, USA.
authors: Changning
authors: C
authors: Li
authors: State University of New York at Buffalo, Department of Biomedical Engineering, Buffalo, New York, 14260, USA.
authors: Julian M
authors: JM
authors: Lippmann
authors: State University of New York at Buffalo, Department of Biomedical Engineering, Buffalo, New York, 14260, USA.
authors: Yun
authors: Y
authors: Wu
authors: State University of New York at Buffalo, Department of Biomedical Engineering, Buffalo, New York, 14260, USA.
authors: Ruogang
authors: R
authors: Zhao
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects; D006801:Humans; D008168:Lung / Q000737:chemistry / Q000187:drug effects*; D035683:MicroRNAs / Q000235:genetics*; D008954:Models, Biological; D037742:Nanotubes, Carbon / Q000633:toxicity*; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D046888:Tissue Array Analysis / Q000295:instrumentation* / Q000379:methods; D015854:Up-Regulation
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species