title: Magnetic molecularly imprinted polymers for the determination of β-agonist residues in milk by ultra high performance liquid chromatography with tandem mass spectrometry.
abstract: A simple, accurate, and highly sensitive analytical method was developed in this study for the determination of nine β-agonists in milk. In this method, a new magnetic adsorbent of molecularly imprinted polymers/magnetic nanoparticles prepared by simple physical blending was adopted, which enabled magnetic solid-phase extraction. Thus, the resultant material can be separated from the solvent rapidly and conveniently by a magnet. Two kinds of molecularly imprinted polymer/magnetic nanoparticles materials were fabricated, and the characteristics of materials such as the ratio, pH, amount, desorption, and regeneration were investigated. The analytes were quantified by ultra high performance liquid chromatography coupled to an electrospray ionization tandem mass spectrometer operating in multiple reaction monitoring modes. The detection limit of the method was 0.003-0.3 μg/L, and the detection capability was 0.01-0.3 μg/L. The recoveries of these compounds were 65.7-114% at three spiked levels. Reproducibility represented by relative standard deviation was 11.2% or less. The method was successfully applied to the screening of real samples obtained from local markets and confirmation of the suspected target analytes.
authors: Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, Kunming, P. R. China.|Laboratory of Quality & Safety Risk Assessment for Agro-Product, Ministry of Agriculture, Kunming, P. R. China.|Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, Kunming, P. R. China.
authors: Hongcheng
authors: H
authors: Liu
authors: Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, Kunming, P. R. China.|Laboratory of Quality & Safety Risk Assessment for Agro-Product, Ministry of Agriculture, Kunming, P. R. China.|Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, Kunming, P. R. China.
authors: Xin
authors: X
authors: Lin
authors: Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, Kunming, P. R. China.|Laboratory of Quality & Safety Risk Assessment for Agro-Product, Ministry of Agriculture, Kunming, P. R. China.|Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, Kunming, P. R. China.
authors: Tao
authors: T
authors: Lin
authors: Kunming Medical University, Kunming, P. R. China.
authors: Yulong
authors: Y
authors: Zhang
authors: Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, Kunming, P. R. China.|Laboratory of Quality & Safety Risk Assessment for Agro-Product, Ministry of Agriculture, Kunming, P. R. China.|Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, Kunming, P. R. China.
authors: Yinglan
authors: Y
authors: Luo
authors: Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, Kunming, P. R. China. cmliu_0@sina.com.|Laboratory of Quality & Safety Risk Assessment for Agro-Product, Ministry of Agriculture, Kunming, P. R. China. cmliu_0@sina.com.|Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, Kunming, P. R. China. cmliu_0@sina.com.
authors: Qiwan
authors: Q
authors: Li
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000302:isolation & purification*; D000327:Adsorption; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis; D057230:Limit of Detection; D008280:Magnetics / Q000295:instrumentation / Q000379:methods*; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D000318:Adrenergic beta-Agonists; D011108:Polymers
keywords: Beta-agonists; Magnetic nanoparticles; Molecularly imprinted polymers; Tandem mass spectrometry