title: [Establishment and Verification of 6-color Fluorescent-labeled Rapid PCR Amplification System].
abstract: OBJECTIVE
  To establish the rapid PCR amplification program and system and to verify the technical indexes.
  
  
  METHODS
  PCR multiplex and capillary electrophoresis detection of 24 autosomal STR loci and one Y-STR loci using the 6-color fluorescence marking technology, as well as A melogenin and Y-InDel. Meanwhile, sensitivity, specificity, identity, stability, mixing and a batch of sample tests were investigated, and the genotype of various routine samples and degraded, exfoliated cell samples were observed.
  
  
  RESULTS
  The sensitivity of the system was 0.062 5 ng. In addition, the genotype could be detected accurately only around 65 min via rapid amplification. The species-specificity was high and the genotyping of all kinds of dry blood specimens of filter paper and mixed, degraded, exfoliated cell samples were accurate.
  
  
  CONCLUSIONS
  The rapid amplification system can significantly improve the detection rate, and obtain accurate and stable genotyping results, which may be important implications for the establishment of STR database and study on population genetics and forensic identification.
authors: Ya-ju
authors: YJ
authors: Liu
authors: Jun-tao
authors: JT
authors: Zhang
authors: Hai-ying
authors: HY
authors: Jin
authors: Mei-sen
authors: MS
authors: Shi
mesh_terms: D019075:Electrophoresis, Capillary; D005453:Fluorescence; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity