title: Rapid simulated gastric fluid digestion of in-seed/grain proteins expressed in genetically engineered crops.
abstract: The speed of simulated gastric digestion of proteins expressed in genetically engineered (GE) crops is commonly used to inform the allergenicity risk assessment. However, persistence of purified proteins in simulated gastric fluid (SGF) is poorly correlated with the allergenic status of proteins. It has been proposed that the plant or food matrix may affect the digestion of proteins and should be considered in interpreting digestion results. Here the SGF digestion of several GE proteins both as purified preparations and in soybean, corn, and cotton seed/grain extracts (in-matrix) are compared. Cry1F, Cry1Ac, phosphinothricin acetyltransferase (PAT), aryloxyalkanoate dioxygenase-1 (AAD-1), aryloxyalkanoate dioxygenase-12 (AAD-12), and double mutant 5-enol pyruvylshikimate-3-phosphate synthase (2mEPSPS) were all found to rapidly digest both as purified protein preparations and in seed/grain extracts from GE crops expressing these proteins. Based on these results, purified protein from microbial sources is a suitable surrogate for proteins in-matrix when conducting SGF digestion studies.
authors: Dow AgroSciences LLC, Indianapolis, IN, 46268, USA.
authors: Barry W
authors: BW
authors: Schafer
authors: Dow AgroSciences LLC, Indianapolis, IN, 46268, USA.
authors: Shawna K
authors: SK
authors: Embrey
authors: Dow AgroSciences LLC, Indianapolis, IN, 46268, USA. Electronic address: raherman@dow.com.
authors: Rod A
authors: RA
authors: Herman
mesh_terms: D000485:Allergens / Q000009:adverse effects / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018556:Crops, Agricultural / Q000009:adverse effects / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004063:Digestion; D005512:Food Hypersensitivity / Q000209:etiology / Q000276:immunology; D059022:Food Safety; D030841:Food, Genetically Modified / Q000009:adverse effects; D005750:Gastric Juice / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D010940:Plant Proteins / Q000009:adverse effects / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000009:adverse effects / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D059748:Proteolysis; D018570:Risk Assessment; D012639:Seeds / Q000009:adverse effects / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000485:Allergens; D010940:Plant Proteins
keywords: Allergenicity; Digestion; Genetically engineered; Genetically modified; In-matrix; Purified proteins; Risk assessment; Simulated gastric fluid; Transgenic