title: RpoE promotes invasion and intracellular survival by regulating SPI-1 and SPI-2 in Salmonella enterica serovar Typhi.
abstract: To demonstrate the role of RpoE during the later stage of hyperosmotic stress in Salmonella.
  
  
  
  Expressions of SPI-1 and SPI-2 under hyperosmotic stress for 120 min were investigated by a microarray, and the invasion and intracellular survival of wild-type and ΔrpoE strains were compared. The global differential expression of bacterial proteins between the wild-type and ΔrpoE strains was examined after 120 min of hyperosmotic stress.
  
  
  
  SPI-1 and SPI-2 were repressed, and the invasion and intracellular survival were defected in the ΔrpoE strain. Thirteen bacterial-associated proteins and 11 secreted proteins differed significantly between the wild-type and ΔrpoE strains.
  
  
  
  RpoE may promote invasion and intracellular survival by regulating the expression of SPI-1 and SPI-2.
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.|School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.|College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.|State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors: Haifang
authors: H
authors: Zhang
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.|School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.|College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.|State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors: Yanwei
authors: Y
authors: Jia
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.|School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.|College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.|State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors: Xiaofang
authors: X
authors: Xie
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.|School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.|College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.|State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors: Min
authors: M
authors: Wang
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.|School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.|College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.|State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors: Yi
authors: Y
authors: Zheng
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.|School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.|College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.|State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors: Shungao
authors: S
authors: Xu
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.|School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.|College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.|State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors: Wei
authors: W
authors: Zhang
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.|School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.|College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.|State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors: Qiang
authors: Q
authors: Wang
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.|School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.|College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.|State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors: Xinxiang
authors: X
authors: Huang
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.|School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.|College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.|State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors: Hong
authors: H
authors: Du
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D050296:Microbial Viability; D012485:Salmonella typhi / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism* / Q000472:pathogenicity; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D014435:Typhoid Fever / Q000382:microbiology*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; C440638:SPI-2 protein, Salmonella; D012808:Sigma Factor; C419942:Spi1 protein, Salmonella
keywords: RpoE; SPI-1; SPI-2; Salmonella enterica serovar Typhi; SipC; hyperosmotic stress