title: Modified glycogen as construction material for functional biomimetic microfibers.
abstract: We describe a conceptually new, microfibrous, biodegradable functional material prepared from a modified storage polysaccharide also present in humans (glycogen) showing strong potential as direct-contact dressing/interface material for wound healing. Double bonds were introduced into glycogen via allylation and were further exploited for crosslinking of the microfibers. Triple bonds were introduced by propargylation and served for further click functionalization of the microfibers with bioactive peptide. A simple solvent-free method allowing the preparation of thick layers was used to produce microfibers (diameter ca 2μm) from allylated and/or propargylated glycogen. Crosslinking of the samples was performed by microtron beta-irradiation, and the irradiation dose was optimized to 2kGy. The results from biological testing showed that these highly porous, hydrophilic, readily functionalizable materials were completely nontoxic to cells growing in their presence. The fibers were gradually degraded in the presence of cells.
authors: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. Electronic address: rabyk@imc.cas.cz.
authors: Mariia
authors: M
authors: Rabyk
authors: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. Electronic address: mhruby@centrum.cz.
authors: Martin
authors: M
authors: Hruby
authors: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. Electronic address: vetrix@seznam.cz.
authors: Miroslav
authors: M
authors: Vetrik
authors: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. Electronic address: jkucka@centrum.cz.
authors: Jan
authors: J
authors: Kucka
authors: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. Electronic address: proks@imc.cas.cz.
authors: Vladimir
authors: V
authors: Proks
authors: Institute of Physiology AS CR, v.v.i., Videnska 1083, 14220 Prague 4, Czech Republic. Electronic address: parizek@biomed.cas.cz.
authors: Martin
authors: M
authors: Parizek
authors: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. Electronic address: konefal@imc.cas.cz.
authors: Rafal
authors: R
authors: Konefal
authors: Nuclear Physics Institute AS CR, v.v.i., 250 68 Rez, Czech Republic. Electronic address: krist@ujf.cas.cz.
authors: Pavel
authors: P
authors: Krist
authors: Nuclear Physics Institute AS CR, v.v.i., 250 68 Rez, Czech Republic. Electronic address: chvatil@ujf.cas.cz.
authors: David
authors: D
authors: Chvatil
authors: Institute of Physiology AS CR, v.v.i., Videnska 1083, 14220 Prague 4, Czech Republic. Electronic address: lucy@biomed.cas.cz.
authors: Lucie
authors: L
authors: Bacakova
authors: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. Electronic address: slouf@imc.cas.cz.
authors: Miroslav
authors: M
authors: Slouf
authors: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. Electronic address: stepan@imc.cas.cz.
authors: Petr
authors: P
authors: Stepanek
mesh_terms: D020341:Absorbable Implants; D001458:Bandages; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D006003:Glycogen / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D011108:Polymers; D006003:Glycogen
keywords: Acetic acid (PubChem CID 176); Allyl bromide (PubChem CID 7841); Fibers; Glycogen; Glycogen (PubChem CID 439177); Irradiation crosslinking; Propargyl bromide (PubChem CID 7842); Sodium hydroxide (PubChem CID 14798); Tissue; Wound healing dressing