title: Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis.
abstract: OBJECTIVE
  The study aimed to investigate key intrafollicular prognostic factors among various cytokines and angiogenic molecules for prediction of mature oocytes and good-quality embryos in women with endometriosis undergoing in vitro fertilization (IVF).
  
  
  METHODS
  Paired follicular fluid and serum samples were collected from 200 women with advanced stage endometriosis and 140 normal ovulating women during oocyte retrieval. The concentrations of cytokines (pro-inflammatory: IL-1β, TNF-α, IL-2, IL-8, IL-12, IFN-γ; anti-inflammatory: IL-4, IL-6, IL-10) and angiogenic molecules (vascular endothelial growth factor (VEGF), adrenomedullin, angiogenin) were determined in follicular fluid and serum using ELISA. Expression of these molecules was subjected to multivariate analysis for the identification of major predictive markers of oocyte and embryo quality. Receiver operating characteristic (ROC) curve was applied to determine the best cutoff point for the discrimination between mature and immature oocytes in these women.
  
  
  RESULTS
  Significant increases in levels of cytokines and angiogenic molecules were observed in women with endometriosis compared to controls (P < 0.001). From the validated partial least squares-discriminant analysis (PLS-DA) model, IL-8, IL-12, and adrenomedullin were identified as the most important factors contributing to endometriosis and were negatively associated with oocyte maturity and embryo quality.
  
  
  CONCLUSIONS
  The levels of IL-8, IL-12, and adrenomedullin may be good indicators of embryo and oocyte quality in endometriosis patients undergoing IVF. Further studies are necessary to ascertain the potential of these markers for oocyte and embryo developmental competence which may help improve the chances of a successful IVF in endometriosis patients.
authors: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India.
authors: Abhay Kumar
authors: AK
authors: Singh
authors: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India.
authors: Mainak
authors: M
authors: Dutta
authors: Institute of Reproductive Medicine, Salt Lake, Kolkata, West Bengal, 700020, India.
authors: Ratna
authors: R
authors: Chattopadhyay
authors: Institute of Reproductive Medicine, Salt Lake, Kolkata, West Bengal, 700020, India.
authors: Baidyanath
authors: B
authors: Chakravarty
authors: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India. koel@smst.iitkgp.ernet.in.
authors: Koel
authors: K
authors: Chaudhury
mesh_terms: D053607:Adrenomedullin / Q000097:blood*; D000328:Adult; D004624:Embryo Transfer / Q000379:methods; D004715:Endometriosis / Q000097:blood* / Q000473:pathology; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000473:pathology; D018664:Interleukin-12 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D054315:Oocyte Retrieval; D009865:Oocytes / Q000378:metabolism / Q000473:pathology
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8; D053607:Adrenomedullin; D018664:Interleukin-12
keywords: Cytokines; Embryos; Endometriosis; Follicular fluid; Oocytes