title: Quercetin and Cisplatin combined treatment altered cell cycle and mitogen activated protein kinase expressions in malignant mesotelioma cells.
abstract: BACKGROUND
  Malignant mesothelioma is a locally aggressive and highly lethal neoplasm of pleural, peritoneal and pericardial mesothelial cells without successful therapy. Previously, we reported that Quercetin in combination with Cisplatin inhibits cell proliferation and activates caspase-9 and -3 enzymes in different malignant mesothelioma cell lines. Moreover, Quercetin + Cisplatin lead to accumulation of both SPC111 and SPC212 cell lines in S phase.
  
  
  METHODS
  In present work, 84 genes involved in cell growth and proliferation have analysed by using RT(2)-PCR array system and protein profile of mitogen activated protein kinase (MAPK) family proteins investigated by western blots.
  
  
  RESULTS
  Our results showed that Quercetin and Quercetin + Cisplatin modulated gene expression of cyclins, cyclin dependent kinases and cyclin dependent kinases inhibitors. In addition genes involved in JNK, p38 and MAPK/ERK pathways were up regulated. Moreover, while p38 and JNK phosphorylations were increased, ERK phosphorylations were decreased after using Quercetin + Cisplatin.
  
  
  CONCLUSIONS
  This research has clarified our previous results and detailed mechanism of anti-carcinogenic potential of Quercetin alone and incombination with Cisplatin on malignant mesothelioma cells.
authors: Department of Molecular Biology & Genetics, Faculty of Science, Gebze Technical University, 41400, Gebze, Kocaeli, Turkey. ademiroglu@gtu.edu.tr.
authors: Asuman
authors: A
authors: Demiroglu-Zergeroglu
authors: Department of Biology, Faculty of Science, Anadolu University, 260470, Eskişehir, Turkey.
authors: Emel
authors: E
authors: Ergene
authors: Department of Molecular Biology & Genetics, Faculty of Science, Gebze Technical University, 41400, Gebze, Kocaeli, Turkey.
authors: Nurettin
authors: N
authors: Ayvali
authors: Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon. Kuetevictor@yahoo.fr.
authors: Victor
authors: V
authors: Kuete
authors: Department of Biology, Faculty of Science, Anadolu University, 260470, Eskişehir, Turkey.
authors: Hulya
authors: H
authors: Sivas
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D008654:Mesothelioma / Q000378:metabolism*; D000086002:Mesothelioma, Malignant; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D011794:Quercetin; D020928:Mitogen-Activated Protein Kinases; D002945:Cisplatin
keywords: Cell cycle; Cisplatin; JNK; MAPK; Mesothelioma; Quercetin; p38