title: 2,3,7,8-Tetrachlorodibenzo-p-dioxin Mediated Cleft palate by Mouse Embryonic Palate Mesenchymal Cells.
abstract: OBJECTIVE
  To evaluate the effects of mouse embryonic palatal mesenchymal (MEPM) cells by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced.
  
  
  METHODS
  An experimental study at Xinxiang Medical University.
  
  
  METHODS
  Primary MEPM cells were derived from palatal tissue on 30 pregnant C57BL/6 female mice (embryonic day13, GD13). The MEPM cells were placed in a humidified incubator at 37°C with 5% CO2atmosphere with media replaced every other day. The third passage cells were seeded, and one part of cells were treated with 10nM TCDD (TCDD group). And others were treated with DMSO (≤0.05%, as control group). After 72h, MTT assay was analysed cell viability. Scratch wound-healing was analysed cell motility. Flow cytometry was analysed cell apoptosis and cycle. Western blot was analysed the expression of cyclinE, CDK2, P16 and P21.
  
  
  RESULTS
  TCDD inhibited the growth and migration of MEPM cells, while increased cell apoptosis. TCDD exposure inhibited the progression of cells from G1 to S phase and tended to reduce the number of cells entering the G2/M phase. TCDD inhibited expression of cyclinE and CDK2 at the protein level, instead increased the expression of P16 and p21 proteins.
  
  
  CONCLUSIONS
  TCDD might induce cleft palate by altering MEPM cells.
authors: School of Public Health, Xinxiang Medical University, 601 Jinsui Road, Xinxiang, Henan, 453003, China; Department of Toxicology, College of Public Health, Zhengzhou University, 100 Science Road, Zhengzhou, Henan, 450001, China. Electronic address: gaoliyun813@126.com.
authors: Liyun
authors: L
authors: Gao
authors: School of Life Sciences and Technology, Xinxiang Medical University, 601 Jinsui Avenue, Xinxiang 453003, China.
authors: Yan
authors: Y
authors: Wang
authors: School of Public Health, Xinxiang Medical University, 601 Jinsui Road, Xinxiang, Henan, 453003, China.
authors: Yongcheng
authors: Y
authors: Yao
authors: School of Public Health, Xinxiang Medical University, 601 Jinsui Road, Xinxiang, Henan, 453003, China.
authors: Guanghui
authors: G
authors: Zhang
authors: School of Public Health, Xinxiang Medical University, 601 Jinsui Road, Xinxiang, Henan, 453003, China.
authors: Guangliang
authors: G
authors: Han
authors: School of Public Health, Xinxiang Medical University, 601 Jinsui Road, Xinxiang, Henan, 453003, China. Electronic address: wdwu2013@126.com.
authors: Weidong
authors: W
authors: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002972:Cleft Palate / Q000139:chemically induced*; D005260:Female; D005434:Flow Cytometry; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D010159:Palate / Q000196:embryology*; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011247:Pregnancy
chemical_list: D000072317:Polychlorinated Dibenzodioxins
keywords: 2,3,7,8-Tetrachlorodibenzo-p-dioxin; Cell apoptosis; Cell cycle; Cell migration; Cell viability; Mouse embryonic palatal mesenchymal cells