title: Expression and Protein Interaction Analyses Reveal Combinatorial Interactions of LBD Transcription Factors During Arabidopsis Pollen Development.
abstract: LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription factor gene family members play key roles in diverse aspects of plant development. LBD10 and LBD27 have been shown to be essential for pollen development in Arabidopsis thaliana. From the previous RNA sequencing (RNA-Seq) data set of Arabidopsis pollen, we identified the mRNAs of LBD22, LBD25 and LBD36 in addition to LBD10 and LBD27 in Arabidopsis pollen. Here we conducted expression and cellular analysis using GFP:GUS (green fluorescent protein:β-glucuronidase) reporter gene and subcellular localization assays using LBD:GFP fusion proteins expressed under the control of their own promoters in Arabidopsis. We found that these LBD proteins display spatially and temporally distinct and overlapping expression patterns during pollen development. Bimolecular fluorescence complementation and GST (glutathione S-transferase) pull-down assays demonstrated that protein-protein interactions occur among the LBDs exhibiting overlapping expression during pollen development. We further showed that LBD10, LBD22, LBD25, LBD27 and LBD36 interact with each other to form heterodimers, which are localized to the nucleus in Arabidopsis protoplasts. Taken together, these results suggest that combinatorial interactions among LBD proteins may be important for their function in pollen development in Arabidopsis.
authors: Department of Bioenergy Science and Technology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 500-757, Korea.
authors: Mirim
authors: M
authors: Kim
authors: Department of Bioenergy Science and Technology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 500-757, Korea.
authors: Min-Jung
authors: MJ
authors: Kim
authors: Department of Bioenergy Science and Technology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 500-757, Korea.
authors: Shashank
authors: S
authors: Pandey
authors: Department of Bioenergy Science and Technology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 500-757, Korea jungmkim@jnu.ac.kr.|Kumho Life Science Laboratory, Chonnam National University, Buk-Gu, Gwangju 500-757, Korea.
authors: Jungmook
authors: J
authors: Kim
mesh_terms: D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D005453:Fluorescence; D005966:Glucuronidase / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008954:Models, Biological; D030821:Plants, Genetically Modified; D011058:Pollen / Q000254:growth & development* / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011523:Protoplasts / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D005966:Glucuronidase
keywords: Arabidopsis; Lateral organ boundaries domain; Male gametophyte; Pollen; Protein–protein interaction; Reproduction