title: Pseudomonas turukhanskensis sp. nov., isolated from oil-contaminated soils.
abstract: A bacterial strain named IB1.1T was isolated in a screening of hydrocarbon-degrading bacteria from oil-contaminated soils on the territory of the Turukhansk District of Krasnoyarsk Krai, East Siberia, Russia. The 16S rRNA gene sequence had 98.7 % identity with respect to the closest phylogenetic relative, Pseudomonas granadensis F-278,770T, and the next most closely related species with 98.6 % similarity was Pseudomonaspunonensis, suggesting that IB1.1T should be classified within the genus Pseudomonas. The analysis of housekeeping genes rpoB, rpoD and gyrB showed similarities lower than 90 % in all cases with respect to the closest relatives, confirming its phylogenetic affiliation. The strain showed a polar flagellum. The respiratory quinone was Q9. The major fatty acids were 16 : 1ω7c/16 : 1ω6c (summed feature 3), 18 : 1ω7c and 16 : 0. The strain was oxidase- and catalase-positive, but the arginine dihydrolase system was not present. Nitrate reduction, urease and β-galactosidase production, and aesculin hydrolysis were negative. The temperature range for growth was 4-34 °C, and the strain could grow at pH 11. The DNA G+C content was 58.5 mol%. DNA-DNA hybridization results showed values of less than 30 % relatedness with respect to the type strains of the eight most closely related species. Therefore, the dataset of genotypic, phenotypic and chemotaxonomic data support the classification of strain IB1.1T into a novel species of the genus Pseudomonas, for which the name Pseudomonasturukhanskensis sp. nov. is proposed. The type strain is IB1.1T (=VKM B-2935T=CECT 9091T).
authors: Laboratory of Biologically Active Agents, Ufa Institute of Biology of the Russian Academy of Sciences, Ufa, Russia.
authors: Tatiana Y
authors: TY
authors: Korshunova
authors: Instituto de Recursos Naturales y Agrobiología, IRNASA-CSIC, Salamanca, Spain.|Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA (CSIC), Salamanca, Spain.
authors: Martha-Helena
authors: MH
authors: Ramírez-Bahena
authors: Laboratory of Biologically Active Agents, Ufa Institute of Biology of the Russian Academy of Sciences, Ufa, Russia.
authors: Sergey P
authors: SP
authors: Chetverikov
authors: Instituto de Recursos Naturales y Agrobiología, IRNASA-CSIC, Salamanca, Spain.|Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA (CSIC), Salamanca, Spain.
authors: Jose M
authors: JM
authors: Igual
authors: Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA (CSIC), Salamanca, Spain.|Instituto de Recursos Naturales y Agrobiología, IRNASA-CSIC, Salamanca, Spain.
authors: Álvaro
authors: Á
authors: Peix
authors: Laboratory of Biologically Active Agents, Ufa Institute of Biology of the Russian Academy of Sciences, Ufa, Russia.
authors: Oleg
authors: O
authors: Loginov
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D059392:Petroleum Pollution; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D017422:Sequence Analysis, DNA; D012800:Siberia; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030571:ubiquinone 9