title: Implementation of high slurry concentration and sonication to pack high-efficiency, meter-long capillary ultrahigh pressure liquid chromatography columns.
abstract: Slurry packing capillary columns for ultrahigh pressure liquid chromatography is complicated by many interdependent experimental variables. Previous results have suggested that combination of high slurry concentration and sonication during packing would create homogeneous bed microstructures and yield highly efficient capillary columns. Herein, the effect of sonication while packing very high slurry concentrations is presented. A series of six, 1m×75μm internal diameter columns were packed with 200mg/mL slurries of 2.02μm bridged-ethyl hybrid silica particles. Three of the columns underwent sonication during packing and yielded highly efficient separations with reduced plate heights as low as 1.05.
authors: Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599-3290, United States.
authors: Justin M
authors: JM
authors: Godinho
authors: Department of Chemistry, Philipps-Universität, Marburg, Hans-Meerwein-Strasse 4, 35032 Marburg, Germany.
authors: Arved E
authors: AE
authors: Reising
authors: Department of Chemistry, Philipps-Universität, Marburg, Hans-Meerwein-Strasse 4, 35032 Marburg, Germany. Electronic address: tallarek@staff.uni-marburg.de.
authors: Ulrich
authors: U
authors: Tallarek
authors: Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599-3290, United States. Electronic address: jj@unc.edu.
authors: James W
authors: JW
authors: Jorgenson
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D011312:Pressure; D012822:Silicon Dioxide / Q000737:chemistry; D013010:Sonication
chemical_list: D012822:Silicon Dioxide
keywords: High efficiency; High slurry concentrations; Packed capillary columns; Sonication; UHPLC