title: Recent applications of capillary electromigration methods to separation and analysis of proteins.
abstract: This review article describes the significant recent developments in analysis of proteins by capillary electromigration (CE) methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography and electrochromatography) during the period 2011-2015. Improvements in sample preparation, preconcentration, suppression of adsorption and control of electroosmotic flow, separations by particular CE methods, and the detection schemes used in the analysis of proteins are discussed. Innovative applications of the above CE methods for quality control of protein biopharmaceuticals, protein determination in complex biomatrices, peptide mapping of proteins, and determination of physicochemical parameters of proteins are presented.
authors: Institute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo n. 2, 166 10 Prague 6, Czech Republic.
authors: Sille
authors: S
authors: Štěpánová
authors: Institute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo n. 2, 166 10 Prague 6, Czech Republic. Electronic address: kasicka@uochb.cas.cz.
authors: Václav
authors: V
authors: Kašička
mesh_terms: D019075:Electrophoresis, Capillary; D011506:Proteins / Q000032:analysis*
chemical_list: D011506:Proteins
keywords: Affinity electrophoresis; Capillary electrophoresis; Isoelectric focusing; Isotachophoresis; Proteins; Review