title: Description of Deinococcus oregonensis sp. nov., from biological soil crusts in the Southwestern arid lands of the United States of America.
abstract: Biological soil crusts are distinct habitats, harbor unique prokaryotic diversity and gave an impetus to isolate novel species. In the present study, a pink-pigmented bacterium, (OR316-6T), was isolated from biological soil crusts using oligotrophic BG11-PGY medium. Strain OR316-6Twas Gram-positive, short rods, non-motile and non-spore forming. Cells were positive for catalase, oxidase and β-galactosidase and negative for most of the enzymatic activities. The major fatty acids present were C16:0, C17:0, and C16:1ω7c and contained MK-8 and MK-10 as the predominant menaquinones. The cell wall peptidoglycan was of A3β variant with L-ornithine as the diamino acid. Based on the above characteristics, strain OR316-6Twas assigned to the genus Deinococcus. The phylogenetic analysis indicated that strain OR316-6Twas closely related to D. aquatilis DSM 23025Twith a 16S rRNA gene similarity of 99.3 % and clustered with a bootstrap value of 100 %. DNA-DNA similarity between strain OR316-6Tand D. aquatilis DSM 23025Twas 37.0 % indicating that strain OR316-6Twas a novel species. Further, DNA fingerprinting of stains OR316-6Tand D. aquatilis DSM 23035Tdemonstrated that both strains were related to each other with a similarity coefficient of only 0.32 and supported the species status to strain OR316-6T. In addition, phenotypic characteristics distinguished strain OR316-6Tfrom D. aquatilis DSM 23025T. Based on the cumulative differences, strain OR316-16Texhibited with its closely related species, it was identified as a novel species and proposed the name Deinococcus oregonensis sp. nov. The type strain is D. oregonensis sp. nov. (OR316-6T= JCM 13503T= DSM 17762T).
authors: School of Life Sciences, Arizona State University, Main Campus, Tempe, AZ, 85287-4501, USA. rukmagsn@gmail.com.|CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500 007, India. rukmagsn@gmail.com.
authors: Sathyanarayana Reddy
authors: SR
authors: Gundlapally
authors: School of Life Sciences, Arizona State University, Main Campus, Tempe, AZ, 85287-4501, USA.
authors: Ferran
authors: F
authors: Garcia-Pichel
mesh_terms: D015373:Bacterial Typing Techniques; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D034301:Deinococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D015206:Southwestern United States; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D001616:beta-Galactosidase
keywords: Biological soil crusts; Deinococcus oregonensis