title: Efficient extraction and preparative separation of four main isoflavonoids from Dalbergia odorifera T. Chen leaves by deep eutectic solvents-based negative pressure cavitation extraction followed by macroporous resin column chromatography.
abstract: In this study, green and efficient deep eutectic solvent-based negative pressure cavitation-assisted extraction (DES-NPCE) followed by macroporous resin column chromatography was developed to extract and separate four main isoflavonoids, i.e. prunetin, tectorigenin, genistein and biochanin A from Dalbergia odorifera T. Chen leaves. The extraction procedure was optimized systematically by single-factor experiments and a Box-Behnken experimental design combined with response surface methodology. The maximum extraction yields of prunetin, tectorigenin, genistein and biochanin A reached 1.204, 1.057, 0.911 and 2.448mg/g dry weight, respectively. Moreover, the direct enrichment and separation of four isoflavonoids in DES extraction solution was successfully achieved by macroporous resin AB-8 with recovery yields of more than 80%. The present study provides a convenient and efficient method for the green extraction and preparative separation of active compounds from plants.
authors: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China.
authors: Lu
authors: L
authors: Li
authors: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China.
authors: Ju-Zhao
authors: JZ
authors: Liu
authors: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China.
authors: Meng
authors: M
authors: Luo
authors: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China.
authors: Wei
authors: W
authors: Wang
authors: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China.
authors: Yu-Yan
authors: YY
authors: Huang
authors: Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany.
authors: Thomas
authors: T
authors: Efferth
authors: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China. Electronic address: whm0709@163.com.
authors: Hui-Mei
authors: HM
authors: Wang
authors: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China; Collaborative Innovation Center for Development and Utilization of Forest Resources, Northeast Forestry University, 150040 Harbin, PR China. Electronic address: yujie_fu@163.com.
authors: Yu-Jie
authors: YJ
authors: Fu
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D035782:Dalbergia / Q000737:chemistry*; D007529:Isoflavones / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D007529:Isoflavones; D010936:Plant Extracts
keywords: Dalbergia odorifera T. Chen; Deep eutectic solvent; Isoflavonoids; Macroporous resin column chromatography; Negative pressure cavitation-assisted extraction