title: Let-7b inhibits the malignant behavior of glioma cells and glioma stem-like cells via downregulation of E2F2.
abstract: Glioblastoma multiforme (GBM), the most common and lethal primary brain tumor in adults characterized by high proliferative ability and mortality rate, contains a small subpopulation of cancer stem-like cells (CSCs), which is responsible for GBM progression and therapeutic resistance. Numerous microRNAs are strongly implicated in the malignancy of glioma. However, their specific functions and roles have yet to be fully demonstrated. In the present study, we revealed that the upregulation of Let-7b, a member of the Let-7 microRNA family, inhibited proliferation, migration, and invasion in glioma cell lines. Using bioinformatics, expression analysis, and luciferase assay, E2F2 was confirmed as a candidate target of Let-7b. Moreover, we also observed that elevated levels of Let-7b resulted in a reduction of tumor sphere growth and stemness of glioma stem-like cells. Furthermore, we found that knockdown of E2F2 expression could reduce the proliferation of glioma and GSCs, while overexpression of E2F2 partially abrogated the inhibitory effect of Let-7b on the proliferation of glioma and GSCs. In conclusion, we suggest that Let-7b could be developed into a promising anticancer target in glioma.
authors: The Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, No. 2 Sipailou Road, Nanjing, Jiangsu Province, 210096, China.
authors: Hang
authors: H
authors: Song
authors: The Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, No. 2 Sipailou Road, Nanjing, Jiangsu Province, 210096, China.
authors: Yao
authors: Y
authors: Zhang
authors: The Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, No. 2 Sipailou Road, Nanjing, Jiangsu Province, 210096, China.
authors: Na
authors: N
authors: Liu
authors: The Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, No. 2 Sipailou Road, Nanjing, Jiangsu Province, 210096, China.
authors: Dongdong
authors: D
authors: Zhang
authors: The Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, No. 2 Sipailou Road, Nanjing, Jiangsu Province, 210096, China.
authors: Chao
authors: C
authors: Wan
authors: Department of Biochemistry and Molecular Biology, Medical School of Southeast University, No. 87 Dingjiaqiao Road, Nanjing, Jiangsu Province, 210009, China.
authors: Sheng
authors: S
authors: Zhao
authors: Department of Biochemistry and Molecular Biology, Medical School of Southeast University, No. 87 Dingjiaqiao Road, Nanjing, Jiangsu Province, 210009, China.
authors: Yan
authors: Y
authors: Kong
authors: The Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, No. 2 Sipailou Road, Nanjing, Jiangsu Province, 210096, China. yld@seu.edu.cn.|Department of Biochemistry and Molecular Biology, Medical School of Southeast University, No. 87 Dingjiaqiao Road, Nanjing, Jiangsu Province, 210009, China. yld@seu.edu.cn.
authors: Liudi
authors: L
authors: Yuan
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D019295:Computational Biology; D050703:E2F2 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D009457:Neuroglia / Q000378:metabolism* / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D018874:Spheroids, Cellular / Q000378:metabolism* / Q000473:pathology
chemical_list: D050703:E2F2 Transcription Factor; C493761:E2F2 protein, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; C487931:mirnlet7 microRNA, human; D008156:Luciferases
keywords: E2F2; Glioma; Glioma stem cell; Let-7b; miRNA