title: Nuclear respiratory factor-1 (NRF-1) regulated hypoxia-inducible factor-1α (HIF-1α) under hypoxia in HEK293T.
abstract: Hypoxia-inducible factor 1α (HIF-1α) is a master regulator of oxygen homeostasis. Under hypoxia, the active HIF1-α subunits are mainly regulated through increased protein stabilization. Little is known concerning HIF-1α transcriptional regulation. Nuclear respiratory factor 1 (NRF-1) is a DNA-binding transcription factor that regulates mitochondrial biogenesis. In this study, we showed that NRF-1was a repressor of HIF-1α. The cellular depletion of NRF-1 by siRNA targeting leads to increased HIF-1αtranscriptional activity. EMSA, ChIP and luciferase activity allowed the identification of two functional NRF-1 binding sites within HIF-1α promoter. This study therefore identifies NRF-1 as a novel regulator of HIF-1α. © 2016 IUBMB Life, 68(9):748-755, 2016.
authors: Department of Biochemistry, 9 Se Yuan Road, Nantong, Jiangsu, 226019, China.
authors: Dan
authors: D
authors: Wang
authors: Department of Biochemistry, 9 Se Yuan Road, Nantong, Jiangsu, 226019, China.
authors: Jie
authors: J
authors: Zhang
authors: Department of Biochemistry, 9 Se Yuan Road, Nantong, Jiangsu, 226019, China.
authors: Yapeng
authors: Y
authors: Lu
authors: Department of Biochemistry, 9 Se Yuan Road, Nantong, Jiangsu, 226019, China.
authors: Qianqian
authors: Q
authors: Luo
authors: Department of Biochemistry, 9 Se Yuan Road, Nantong, Jiangsu, 226019, China.
authors: Li
authors: L
authors: Zhu
mesh_terms: D001665:Binding Sites / Q000235:genetics; D015687:Cell Hypoxia / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis* / Q000235:genetics; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism; D051266:Nuclear Respiratory Factor 1 / Q000037:antagonists & inhibitors / Q000235:genetics*; D010100:Oxygen / Q000378:metabolism; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C495633:NRF1 protein, human; D051266:Nuclear Respiratory Factor 1; D034741:RNA, Small Interfering; D010100:Oxygen
keywords: gene transcription regulation; hypoxia-inducible factor 1α; nuclear respiratory factor-1