title: Endoinulinase production by a new endoinulinase producer Aspergillus tritici BGPUP6 using a low cost substrate.
abstract: Endoinulinase is an inulolytic enzyme which is used for the production of fructooligosaccharides from inulin. A new endoinulinase producing fungal strain BGPUP6 was identified as Aspergillus tritici on the basis of its molecular characterization. Response surface methodology was used to optimize the endoinulinase production at shake-flask level from Aspergillus tritici BGPUP6 using raw Asparagus inulin as carbon source. Four independent variables (raw inulin, 2-4%; peptone, 0.3-0.7%; (NH4)H2PO4, 0.3-0.7% and pH, 4.5-6.5) were selected for the study based on the CCRD model of RSM. The other media supplements (FeSO4·7H2O, 0.001%; MgSO4·7H2O, 0.05% and KCl, 0.02%) were kept constant in the production medium, throughout the study. Endoinulinase production and biomass yield were selected as dependent responses. The optimal combination of media ingredients obtained from the study was 3% raw inulin, 0.5% peptone, 0.5% (NH4)H2PO4and pH 5.5. Using the optimized media constituents, maximum endoinulinase production (25.01 IU/mL) and biomass yield (0.514g dry weight/50 mL) obtained were in good agreement with the predicted values. Crude enzyme produced was also used for the hydrolysis of inulin. The hydrolysate showed the presence of a mixture of fructooligosaccharides with varied degree of polymerization. This is the first report on the production of an endoinulinase from Aspergillus tritici.
authors: Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, Punjab 147 002, India. Electronic address: rssingh11@lycos.com.
authors: Ram Sarup
authors: RS
authors: Singh
authors: Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, Punjab 147 002, India.
authors: Rupinder Pal
authors: RP
authors: Singh
authors: Chembiotech Laboratories, Advanced Science and Technology Institute, 5 The Croft, Buntsford Drive, Stoke Heath, Bromsgrove, Worcs B60 4JE, UK.
authors: John F
authors: JF
authors: Kennedy
mesh_terms: D027761:Asparagus Plant / Q000737:chemistry; D001230:Aspergillus / Q000378:metabolism*; D018533:Biomass; D005163:Factor Analysis, Statistical; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007218:Industrial Microbiology; D007444:Inulin / Q000737:chemistry*; D007700:Kinetics; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D010461:Peptones / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D005656:Fungal Proteins; D009844:Oligosaccharides; D010461:Peptones; D010710:Phosphates; C024788:ammonium phosphate; C116580:fructooligosaccharide; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase
keywords: Aspergillus tritici; Endoinulinase; Fructooligosaccharides; Inulin; Response surface methodology