title: Involvement of cholinergic and purinergic systems during the inflammatory response caused by Aeromonas hydrophila in Rhamdia quelen.
abstract: The aim of this study was to evaluate the cholinergic (acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)) and purinergic (adenosine deaminase (ADA)) systems in head kidney, spleen, total blood and serum samples in experimentally infected fish with A. hydrophila, and the involvement of these systems during the inflammatory process. Silver catfish (Rhamdia quelen) juveniles were divided into two groups with seven fish each: uninfected (negative control) and infected (positive control). On day 2 post-infection, animals were euthanized and the head kidney, spleen, total blood and serum were collected. AChE and ADA activities in head kidney and spleen decreased in infected animals compared to uninfected animals, as well as AChE in total blood and seric ADA activities. BChE activity was not expressed in the evaluated tissues. Therefore, our results lead to the hypothesis that cholinergic and purinergic systems play an important role on the immune response against A. hydrophila with an anti-inflammatory effect. In summary, AChE and ADA activities reduced probably in order to protect against tissue inflammatory damage caused by infection.
authors: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: matheusd.biomed@yahoo.com.br.
authors: Matheus D
authors: MD
authors: Baldissera
authors: Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors: Carine F
authors: CF
authors: Souza
authors: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors: Pedro Henrique
authors: PH
authors: Doleski
authors: Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors: Guerino B
authors: GB
authors: Júnior
authors: Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, RS, Brazil.
authors: Agueda C
authors: AC
authors: de Vargas
authors: Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: bbaldisserotto@hotmail.com.
authors: Bernardo
authors: B
authors: Baldisserotto
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis*; D000243:Adenosine Deaminase / Q000032:analysis*; D016980:Aeromonas hydrophila / Q000472:pathogenicity*; D000818:Animals; D002091:Butyrylcholinesterase / Q000032:analysis*; D002397:Catfishes; D004195:Disease Models, Animal; D005393:Fish Diseases / Q000473:pathology*; D016905:Gram-Negative Bacterial Infections / Q000473:pathology*; D007249:Inflammation / Q000473:pathology*; D007668:Kidney / Q000473:pathology; D044967:Serum / Q000201:enzymology; D013154:Spleen / Q000473:pathology; D013997:Time Factors
chemical_list: D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D000243:Adenosine Deaminase
keywords: Acetylcholine; Adenosine deaminase; Fish; Infectious diseases