title: Tyrosinase inhibitory flavonoid from Juniperus communis fruits.
abstract: The fruits of Juniperus communis have been traditionally used in the treatment of skin diseases. In our preliminary experiment, the MeOH extract of J. communis effectively suppressed mushroom tyrosinase activity. Three monoflavonoids and five biflavonoids were isolated from J. communis by bioassay-guided isolation and their inhibitory effect against tyrosinase was evaluated. According to the results of all isolates, hypolaetin 7-O-β-xylopyranoside isolated from J. communis exhibited most potent effect of decreasing mushroom tyrosinase activity with an IC50value of 45.15 μM. Further study provided direct experimental evidence for hypolaetin 7-O-β-D-xylopyranoside-attenuated tyrosinase activity in α-MSH-stimulated B16F10 murine melanoma cell. Hypolaetin 7-O-β-D-xylopyranoside from the EtOAc fraction of J. communis was also effective at suppressing α-MSH-induced melanin synthesis. This is the first report of the enzyme tyrosinase inhibition by J. communis and its constituent. Therapeutic attempts with J. communis and its active component, hypolaetin 7-O-β-D-xylopyranoside, might be useful in treating melanin pigmentary disorders.
authors: a College of Pharmacy , Pusan National University , Busan , South Korea.
authors: Jonghwan
authors: J
authors: Jegal
authors: a College of Pharmacy , Pusan National University , Busan , South Korea.
authors: Sang-A
authors: SA
authors: Park
authors: a College of Pharmacy , Pusan National University , Busan , South Korea.
authors: KiWung
authors: K
authors: Chung
authors: a College of Pharmacy , Pusan National University , Busan , South Korea.
authors: Hae Young
authors: HY
authors: Chung
authors: a College of Pharmacy , Pusan National University , Busan , South Korea.
authors: Jaewon
authors: J
authors: Lee
authors: b Department of Agronomy & Medicinal Plant Resources , College of Life Sciences and Natural Resources, Gyeongnam National University of Science and Technology , Jinju , South Korea.
authors: Eun Ju
authors: EJ
authors: Jeong
authors: c Natural Product Laboratory, School of Pharmacy , Sungkyunkwan University , Suwon , South Korea.
authors: Ki Hyun
authors: KH
authors: Kim
authors: a College of Pharmacy , Pusan National University , Busan , South Korea.
authors: Min Hye
authors: MH
authors: Yang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D018653:Juniperus / Q000737:chemistry*; D008543:Melanins / Q000378:metabolism; D051379:Mice; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D000521:alpha-MSH / Q000494:pharmacology
chemical_list: D005419:Flavonoids; D008543:Melanins; D000521:alpha-MSH; D014442:Monophenol Monooxygenase
keywords: Juniperus communis; flavonoids; hypolaetin 7-O-β-D-xylopyranoside; melanin content; tyrosinase inhibitor