title: Shrimp with knockdown of LvSOCS2, a negative feedback loop regulator of JAK/STAT pathway in Litopenaeus vannamei, exhibit enhanced resistance against WSSV.
abstract: JAK/STAT pathway is one of cytokine signaling pathways and mediates diversity immune responses to protect host from viral infection. In this study, LvSOCS2, a member of suppressor of cytokine signaling (SOCS) families, has been cloned and identified from Litopenaeus vannamei. The full length of LvSOCS2 is 1601 bp, including an 1194 bp open reading frame (ORF) coding for a putative protein of 397 amino acids with a calculated molecular weight of ∼42.3 kDa. LvSOCS2 expression was most abundant in gills and could respond to the challenge of LPS, Vibrio parahaemolyticus, Staphhylococcus aureus, Poly (I: C) and white spot syndrome virus (WSSV). There are several STAT binding motifs presented in the proximal promoter region of LvSOCS2 and its expression was induced by LvJAK or LvSTAT protein in a dose dependent manner, suggesting LvSOCS2 could be the transcriptional target gene of JAK/STAT pathway. Moreover, the transcription of DmVir-1, a read out of the activation of JAK/STAT pathway in Drosophila, was promoted by LvJAK but inhibited by LvSOCS2, indicating that LvSOCS2 could be a negative regulator in this pathway and thus can form a negative feedback loop. Our previous study indicated that shrimp JAK/STAT pathway played a positive role against WSSV. In this study, RNAi-mediated knockdown of LvSOCS2 shrimps showed lower susceptibility to WSSV infection and caused lessened virus loads, which further demonstrated that the JAK/STAT pathway could function as an anti-viral immunity in shrimp.
authors: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China.
authors: Sheng
authors: S
authors: Wang
authors: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China.
authors: Xuan
authors: X
authors: Song
authors: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China.
authors: Zijian
authors: Z
authors: Zhang
authors: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China.
authors: Haoyang
authors: H
authors: Li
authors: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China.
authors: Kai
authors: K
authors: Lǚ
authors: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China.
authors: Bin
authors: B
authors: Yin
authors: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China; School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China; South China Sea Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC), PR China. Electronic address: lsshjg@mail.sysu.edu.cn.
authors: Jianguo
authors: J
authors: He
authors: MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China; School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China; South China Sea Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC), PR China. Electronic address: lichaozh@mail2.sysu.edu.cn.
authors: Chaozheng
authors: C
authors: Li
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000276:immunology*; D025461:Feedback, Physiological; D055785:Gene Knockdown Techniques; D007113:Immunity, Innate / Q000235:genetics; D053612:Janus Kinases / Q000378:metabolism; D033561:Penaeidae / Q000276:immunology*; D050791:STAT Transcription Factors / Q000378:metabolism; D015398:Signal Transduction; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000378:metabolism*; D046848:White spot syndrome virus 1 / Q000276:immunology*
chemical_list: D060829:Arthropod Proteins; D050791:STAT Transcription Factors; C491409:Socs2 protein, mouse; D050826:Suppressor of Cytokine Signaling Proteins; D053612:Janus Kinases
keywords: JAK/STAT pathway; Litopenaeus vannamei; Negative feedback loop; SOCS2; WSSV