title: Oral Squamous Carcinoma Cells Express B7-H1 and B7-DC Receptors in Vivo.
abstract: B7-H1 and B7-DC ligands are members of the B7 family with important regulatory functions in cell-mediated immune response. Both receptors are ligands of the programmed death receptor PD-1. B7-H1 expression has been detected in the majority of human carcinomas in vivo. B7-H1 mediated signals are able to negatively regulate activated T cell functions and survival, and enable tumor cells to overcome host response. The aim of this study was to investigate the expression of B7-H1 and B7-DC proteins in oral squamous cell carcinomas (OSCC) in vivo. Tissues from 15 samples were cryo-sected and following histological routine staining (HE), incubated with antibodies against human B7-H1 and B7-DC. Immuno-staining of pan-cytokeratin was performed to ascertain the epithelial origin of the tissue and CK 19 to demonstrate the proliferating stage. Confocal laser scanning microscopy confirmed the presence of both B7-H1 and B7-DC in all 15 OSCC. The B7-H1 and B7-DC staining was located in areas of the tissue that were identified as cancerous lesions in the previously stained HE sections before. Staining with Pan-CK and CK19 provided evidence for the epithelial origin and the proliferating stage of the tissue. The in vivo expression of the B7-H1 and B7-DC receptors in oral squamous cell carcinomas suggest that general mechanisms for immune evasion of tumors are also found in OSCC.
authors: Department of Periodontology, Justus-Liebig-University, Schlangenzahl 14, 35392, Giessen, Germany. Sabine.E.Groeger@dentist.med.uni-giessen.de.
authors: Sabine
authors: S
authors: Groeger
authors: Oral and Maxillofacial Surgery, Justus-Liebig-University, Giessen, Germany.
authors: H P
authors: HP
authors: Howaldt
authors: Institute for Medical Microbiology and Hygiene, Phillips University, Marburg, Germany.
authors: H
authors: H
authors: Raifer
authors: Institute for Pathology, Justus-Liebig-University, Giessen, Germany.
authors: S
authors: S
authors: Gattenloehner
authors: Institute for Medical Microbiology, Justus-Liebig-University, Giessen, Germany.
authors: T
authors: T
authors: Chakraborty
authors: Department of Periodontology, Justus-Liebig-University, Schlangenzahl 14, 35392, Giessen, Germany.
authors: J
authors: J
authors: Meyle
mesh_terms: D000328:Adult; D000368:Aged; D060890:B7-H1 Antigen / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D006801:Humans; D008024:Ligands; D008213:Lymphocyte Activation / Q000502:physiology; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism*; D061027:Programmed Cell Death 1 Ligand 2 Protein / Q000378:metabolism*; D061026:Programmed Cell Death 1 Receptor / Q000378:metabolism
chemical_list: D060890:B7-H1 Antigen; C423236:CD274 protein, human; D008024:Ligands; D008562:Membrane Glycoproteins; D061027:Programmed Cell Death 1 Ligand 2 Protein; D061026:Programmed Cell Death 1 Receptor
keywords: B7-Dc; B7-H1; Immune evasion; In vivo; Oral squamous cell carcinomas