title: Wnt5a Suppresses β-catenin Signaling during Hair Follicle Regeneration.
abstract: Hair follicles display periodic growth. Wnt signaling is a critical regulator for hair follicle regeneration. Previously, we reported that Wnt5a inhibits the telogen-to-anagen transition of hair follicles, but the mechanism by which this process occurs has not yet been reported. Here, we determined the expression patterns of Wnt signaling pathway molecules by quantitative reverse transcription polymerase chain reaction, western blot, and immunohistochemistry and found that β-catenin signaling was suppressed by Wnt5a. We then compared the phenotypes and expression patterns following β-catenin knockdown and Wnt5a overexpression during hair follicle regeneration induced by hair depilation and observed similar patterns. In addition, we performed a rescue experiment in the JB6 cell line and found that the inhibitory effect of Wnt5a on cell proliferation could be rescued by the addition of Wnt3a. Our data reveal that Wnt5a suppresses the activation of β-catenin signaling during hair follicle regeneration.
authors: Department of Cell Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
authors: Yizhan
authors: Y
authors: Xing
authors: Department of Cell Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
authors: Xiaogen
authors: X
authors: Ma
authors: Department of Cell Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
authors: Haiying
authors: H
authors: Guo
authors: Department of Cell Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
authors: Fang
authors: F
authors: Deng
authors: Department of Cell Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
authors: Jin
authors: J
authors: Yang
authors: Department of Cell Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
authors: Yuhong
authors: Y
authors: Li
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018859:Hair Follicle / Q000254:growth & development* / Q000378:metabolism; D006801:Humans; D012038:Regeneration / Q000235:genetics; D060449:Wnt Signaling Pathway / Q000235:genetics; D000071818:Wnt-5a Protein / Q000096:biosynthesis* / Q000235:genetics; D060509:Wnt3A Protein / Q000096:biosynthesis* / Q000235:genetics; D051176:beta Catenin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C555510:WNT3A protein, human; C085389:WNT5A protein, human; D000071818:Wnt-5a Protein; D060509:Wnt3A Protein; D051176:beta Catenin
keywords: Wnt signaling pathway; Wnt5a; hair follicle; regeneration.; β-catenin