title: FRET-Based Mito-Specific Fluorescent Probe for Ratiometric Detection and Imaging of Endogenous Peroxynitrite: Dyad of Cy3 and Cy5.
abstract: Peroxynitrite (OONO(-)) is profoundly implicated in health and disease. The physiological and pathological outcome of OONO(-) is related to its local concentration, and hence, a reliable OONO(-) assay is highly desired. We have developed a FRET-based small-molecule fluorescent probe (PNCy3Cy5), harnessing the differential reactivity of Cy3 and Cy5 toward OONO(-) by fine-tuning. It exhibits high detection sensitivity and yields a ratiometric fluorescent signal. We have exemplified that it can be applied in semiquantitative determination of OONO(-) in living cells. Notably, it specifically localizes in mitochondria, where endogenous OONO(-) is predominantly generated. Thus, PNCy3Cy5 is a promising molecular tool for peroxynitrite biology.
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, and ‡Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology , Meilong Road 130, Shanghai 200237, China.
authors: Xiaotong
authors: X
authors: Jia
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, and ‡Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology , Meilong Road 130, Shanghai 200237, China.
authors: Qiangqiang
authors: Q
authors: Chen
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, and ‡Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology , Meilong Road 130, Shanghai 200237, China.
authors: Yingfang
authors: Y
authors: Yang
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, and ‡Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology , Meilong Road 130, Shanghai 200237, China.
authors: Yao
authors: Y
authors: Tang
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, and ‡Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology , Meilong Road 130, Shanghai 200237, China.
authors: Rui
authors: R
authors: Wang
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, and ‡Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology , Meilong Road 130, Shanghai 200237, China.
authors: Yufang
authors: Y
authors: Xu
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, and ‡Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology , Meilong Road 130, Shanghai 200237, China.
authors: Weiping
authors: W
authors: Zhu
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, and ‡Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology , Meilong Road 130, Shanghai 200237, China.
authors: Xuhong
authors: X
authors: Qian
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D061848:Optical Imaging; D030421:Peroxynitrous Acid / Q000737:chemistry / Q000378:metabolism*; D000067996:RAW 264.7 Cells
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C089187:cyanine dye 3; C085321:cyanine dye 5; D030421:Peroxynitrous Acid