title: Self-Aggregating Deep Cavitand Acts as a Fluorescence Displacement Sensor for Lysine Methylation.
abstract: A dual-mode aggregative host:guest indicator displacement sensing system has been created for the detection of trimethylated peptides and determination of histone demethylase activity. The combination of selective recognition of suitably sized trimethylammonium salts and reversible lipophilic aggregation of the host:guest complex provides a unique quenching mechanism that is not only dependent on affinity for sensitivity but the lipophilicity of the indicator. In addition, aggregation can be controlled by the application of chaotropic anions in the mixture, allowing a second level of discrimination between hard lysine groups and softer trimethyllysines.
authors: Department of Chemistry and ‡Environmental Toxicology Program, University of California-Riverside , Riverside, California 92521, United States.
authors: Yang
authors: Y
authors: Liu
authors: Department of Chemistry and ‡Environmental Toxicology Program, University of California-Riverside , Riverside, California 92521, United States.
authors: Lizeth
authors: L
authors: Perez
authors: Department of Chemistry and ‡Environmental Toxicology Program, University of California-Riverside , Riverside, California 92521, United States.
authors: Magi
authors: M
authors: Mettry
authors: Department of Chemistry and ‡Environmental Toxicology Program, University of California-Riverside , Riverside, California 92521, United States.
authors: Connor J
authors: CJ
authors: Easley
authors: Department of Chemistry and ‡Environmental Toxicology Program, University of California-Riverside , Riverside, California 92521, United States.
authors: Richard J
authors: RJ
authors: Hooley
authors: Department of Chemistry and ‡Environmental Toxicology Program, University of California-Riverside , Riverside, California 92521, United States.
authors: Wenwan
authors: W
authors: Zhong