title: Methods to Study Centrosomes and Cilia in Drosophila.
abstract: Centrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile, and intraflagellar transport (IFT)-independent or IFT-dependent assembly. Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.
authors: Instituto Gulbenkian de Ciência, Rua da Quinta Grande, 6, 2780-156, Oeiras, Portugal. swadhin1cal@gmail.com.
authors: Swadhin Chandra
authors: SC
authors: Jana
authors: Instituto Gulbenkian de Ciência, Rua da Quinta Grande, 6, 2780-156, Oeiras, Portugal.
authors: Susana
authors: S
authors: Mendonça
authors: Instituto Gulbenkian de Ciência, Rua da Quinta Grande, 6, 2780-156, Oeiras, Portugal.
authors: Sascha
authors: S
authors: Werner
authors: Instituto Gulbenkian de Ciência, Rua da Quinta Grande, 6, 2780-156, Oeiras, Portugal. mdias@igc.gulbenkian.pt.
authors: Monica
authors: M
authors: Bettencourt-Dias
mesh_terms: D000818:Animals; D001692:Biological Transport; D015415:Biomarkers; D018385:Centrosome / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism*; D004330:Drosophila / Q000502:physiology*; D015870:Gene Expression; D017930:Genes, Reporter; D011993:Recombinant Fusion Proteins
chemical_list: D015415:Biomarkers; D011993:Recombinant Fusion Proteins
keywords: Basal body; Centrosome; Cilia; Drosophila; Intraflagellar transport; Sensory neuron; Sperm