title: Isolation and characterization of yeasts from fermented apple bagasse as additives for ruminant feeding.
abstract: Solid-state fermentation can be used to produce feeds for ruminants, which can provide an enriched population of yeasts to improve ruminal fermentation. Fermentation of apple bagasse was performed to obtain a yeast-rich product, with the objective of isolating, identifying, and characterizing yeast strains and testing their capability to enhance in vitro ruminal fermentation of fibrous feeds. Yeasts were isolated from apple bagasse fermented under in vitro conditions, using rumen liquor obtained from cannulated cows and alfalfa as a fibrous substrate. A total of 16 new yeast strains were isolated and identified by biochemical and molecular methods. The strains were designated Levazot, followed by the isolate number. Their fermentative capacity was assessed using an in vitro gas production method. Strain Levazot 15 (Candida norvegensis) showed the greatest increase in gas production (p<0.05) compared with the yeast-free control and positively affected in vitro ruminal fermentation parameters of alfalfa and oat straw. Based on these results, it was concluded that the Levazot 15 yeast strain could be potentially used as an additive for ruminants consuming high-fiber diets. However, further studies of effects of these additives on rumen digestion, metabolism, and productive performance of ruminants are required.
authors: Universidad Autonoma de Cd. Juarez, Division Multidisciplinaria, Departamento de Medicina Veterinaria, Nuevo Casas Grandes, Mexico.
authors: Y
authors: Y
authors: Castillo-Castillo
authors: Universidad Autonoma de Chihuahua, Facultad de Zootecnia y Ecologia, Chihuahua, Mexico. Electronic address: oscarruiz80@gmail.com.
authors: O
authors: O
authors: Ruiz-Barrera
authors: Universidad Autonoma de Chihuahua, Facultad de Zootecnia y Ecologia, Chihuahua, Mexico.
authors: M E
authors: ME
authors: Burrola-Barraza
authors: Instituto de Ciencia Animal (ICA), San José de las Lajas, Cuba.
authors: Y
authors: Y
authors: Marrero-Rodriguez
authors: Universidad Autonoma de Tamaulipas, Facultad de Medicina Veterinaria y Zootecnia, Cd. Victoria, Mexico.
authors: J
authors: J
authors: Salinas-Chavira
authors: Universidad Autonoma de Sinaloa, Culiacan, Facultad de Medicina Veterinaria y Zootecnia, Sin, Mexico.
authors: C
authors: C
authors: Angulo-Montoya
authors: Universidad Autonoma de Chihuahua, Facultad de Zootecnia y Ecologia, Chihuahua, Mexico.
authors: A
authors: A
authors: Corral-Luna
authors: Universidad Autonoma de Chihuahua, Facultad de Zootecnia y Ecologia, Chihuahua, Mexico.
authors: C
authors: C
authors: Arzola-Alvarez
authors: Universidad Autonoma de Cd. Juarez, Division Multidisciplinaria, Departamento de Medicina Veterinaria, Nuevo Casas Grandes, Mexico.
authors: M
authors: M
authors: Itza-Ortiz
authors: Universidad Autonoma de Chihuahua, Facultad de Zootecnia y Ecologia, Chihuahua, Mexico.
authors: J
authors: J
authors: Camarillo
mesh_terms: D000821:Animal Feed / Q000382:microbiology*; D000818:Animals; D002482:Cellulose; D005285:Fermentation; D005503:Food Additives; D027845:Malus; D010802:Phylogeny; D012418:Ruminants; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D005503:Food Additives; D002482:Cellulose; C027433:bagasse
keywords: Apple bagasse; Fermentation; Ruminal; Yeast