title: Development of new method and protocol for cryopreservation related to embryo and oocytes freezing in terms of fertilization rate: A comparative study including review of literature.
abstract: BACKGROUND
  Cryopreservation is basically related to meritorious thin samples or small clumps of cells that are cooled quickly without loss. Our main objective is to establish and formulate an innovative method and protocol development for cryopreservation as a gold standard for clinical uses in laboratory practice and treatment. The knowledge regarding usefulness of cryopreservation in clinical practice is essential to carry forward the clinical practice and research.
  
  
  METHODS
  We are trying to compare different methods of cryopreservation (in two dozen of cells) at the same time we compare the embryo and oocyte freezing interms of fertilization rate according to the International standard protocol.
  
  
  RESULTS
  The combination of cryoprotectants and regimes of rapid cooling and rinsing during warming often allows successful cryopreservation of biological materials, particularly cell suspensions or thin tissue samples. Examples include semen, blood, tissue samples like tumors, histological cross-sections, human eggs and human embryos. Although presently many studies have reported that the children born from frozen embryos or "frosties," show consistently positive results with no increase in birth defects or development abnormalities is quite good enough and similar to our study (50-85%).
  
  
  CONCLUSIONS
  We ensure that cryopreservation technology provided useful cell survivability, tissue and organ preservation in a proper way. Although it varies according to different laboratory conditions, it is certainly beneficial for patient's treatment and research. Further studies are needed for standardization and development of new protocol.
authors: Department of Pediatric Surgery, AIIMS, New Delhi, India.
authors: Mayadhar
authors: M
authors: Barik
authors: Department of Pediatric Surgery, AIIMS, New Delhi, India.
authors: Minu
authors: M
authors: Bajpai
authors: Department of Ocular Pharmacology and Pharmacy, AIIMS, New Delhi, India.
authors: Santosh
authors: S
authors: Patnaik
authors: Department of Anatomy, AIIMS, Bhubaneshwar, Odisha, India.
authors: Pravash
authors: P
authors: Mishra
authors: Department of Centre for Community Medicine, AIIMS, New Delhi, India.
authors: Priyamadhaba
authors: P
authors: Behera
authors: Department of Biostatistics, AIIMS, New Delhi, India.
authors: Sada Nanda
authors: SN
authors: Dwivedi
keywords: Autologous bone marrow; cryopreservation; human oocytes; stem cells; transplant; unfertilized oocytes