title: Effect of extremely low frequency electromagnetic field on MAP2 and Nestin gene expression of hair follicle dermal papilla cells.
abstract: BACKGROUND
  In recent years, the extremely low frequency electromagnetic field (ELF-EMF) has attracted a great deal of scientific interest. The ELF-EMF signal is able to control ion transport across ion channels and therefore induce cell differentiation.
  
  
  OBJECTIVE
  The purpose of this study was to investigate the effect of ELF-EMF (50 Hz, 1 mT) on MAP2 and Nestin gene expression of dermal papilla mesenchymal cells (DPCs).
  
  
  METHODS
  In order to examine the effect of chemical and electromagnetic factors on gene expression, 4 experimental groups, namely chemical (cell exposure to chemical signals), EMF (exposing cells to ELF-EMF), chemical-EMF (subjecting cells to chemical signals and ELF-EMF) and control (with no treatment) groups, were prepared, treated for 5 days, and studied. To assess the effect of extended test time on the expression of neural differentiation markers (Nestin and MAP2), an EMF group was prepared and treated for a period of 14 consecutive days. The beneficial role of EMF in inducing neural differentiation was shown by real-time PCR analysis.
  
  
  RESULTS
  The higher expression of MAP2 after 14 days compared to that after 5 days and decrease of cell proliferation on days 5 to 20 were indicative of the positive effect of extending treatment time on neural differentiation by evaluation of gene expression in EMF group.
authors: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran - Iran.|Department of Biomedical Engineering, Amirkabir University of Technology, Tehran - Iran.
authors: Marzie
authors: M
authors: Moraveji
authors: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran - Iran.
authors: Nooshin
authors: N
authors: Haghighipour
authors: Department of Biomedical Engineering, Amirkabir University of Technology, Tehran - Iran.
authors: Hamid
authors: H
authors: Keshvari
authors: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran - Iran.
authors: Tannaz
authors: T
authors: Nourizadeh Abbariki
authors: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran - Iran.
authors: Mohammad Ali
authors: MA
authors: Shokrgozar
authors: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran - Iran.
authors: Amir
authors: A
authors: Amanzadeh
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D004574:Electromagnetic Fields; D005786:Gene Expression Regulation / Q000528:radiation effects*; D018859:Hair Follicle / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000528:radiation effects; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D064231:Nestin / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D008869:Microtubule-Associated Proteins; D064231:Nestin