title: The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis.
abstract: Upon DNA damage, cyclin-dependent kinases (CDKs) are typically inhibited to block cell division. In many organisms, however, it has been found that CDK activity is required for DNA repair, especially for homology-dependent repair (HR), resulting in the conundrum how mitotic arrest and repair can be reconciled. Here, we show that Arabidopsis thaliana solves this dilemma by a division of labor strategy. We identify the plant-specific B1-type CDKs (CDKB1s) and the class of B1-type cyclins (CYCB1s) as major regulators of HR in plants. We find that RADIATION SENSITIVE 51 (RAD51), a core mediator of HR, is a substrate of CDKB1-CYCB1 complexes. Conversely, mutants in CDKB1 and CYCB1 fail to recruit RAD51 to damaged DNA CYCB1;1 is specifically activated after DNA damage and we show that this activation is directly controlled by SUPPRESSOR OF GAMMA RESPONSE 1 (SOG1), a transcription factor that acts similarly to p53 in animals. Thus, while the major mitotic cell-cycle activity is blocked after DNA damage, CDKB1-CYCB1 complexes are specifically activated to mediate HR.
authors: Department of Molecular Mechanisms of Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du CNRS, IBMP-CNRS UPR2357, Université de Strasbourg, Strasbourg Cedex, France.
authors: Annika K
authors: AK
authors: Weimer
authors: Department of Molecular Mechanisms of Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du CNRS, IBMP-CNRS UPR2357, Université de Strasbourg, Strasbourg Cedex, France.
authors: Sascha
authors: S
authors: Biedermann
authors: RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Japan.
authors: Hirofumi
authors: 0000-0003-3370-4111
authors: H
authors: Harashima
authors: Institut für Biologie III, Universität Freiburg, Freiburg, Germany.
authors: Farshad
authors: F
authors: Roodbarkelari
authors: Plant Growth Regulation Laboratory, Nara Institute of Science and Technology, Graduate School of Biological Sciences, Ikoma, Nara, Japan.
authors: Naoki
authors: N
authors: Takahashi
authors: School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
authors: Julia
authors: J
authors: Foreman
authors: Department of Molecular Mechanisms of Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du CNRS, IBMP-CNRS UPR2357, Université de Strasbourg, Strasbourg Cedex, France.
authors: Yonsheng
authors: Y
authors: Guan
authors: Department of Developmental Biology, Biozentrum Klein Flottbek, University of Hamburg, Hamburg, Germany.
authors: Gaëtan
authors: G
authors: Pochon
authors: Department of Developmental Biology, Biozentrum Klein Flottbek, University of Hamburg, Hamburg, Germany.
authors: Maren
authors: 0000-0002-1070-9107
authors: M
authors: Heese
authors: Department of Plant Systems Biology, VIB, Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
authors: Daniël
authors: D
authors: Van Damme
authors: RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Japan.
authors: Keiko
authors: K
authors: Sugimoto
authors: Max-Planck-Institut für Pflanzenzüchtungsforschung, Köln, Germany.
authors: Csaba
authors: C
authors: Koncz
authors: School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
authors: Peter
authors: P
authors: Doerner
authors: Plant Growth Regulation Laboratory, Nara Institute of Science and Technology, Graduate School of Biological Sciences, Ikoma, Nara, Japan JST, CREST, Ikoma, Nara, Japan.
authors: Masaaki
authors: M
authors: Umeda
authors: Department of Molecular Mechanisms of Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du CNRS, IBMP-CNRS UPR2357, Université de Strasbourg, Strasbourg Cedex, France Department of Developmental Biology, Biozentrum Klein Flottbek, University of Hamburg, Hamburg, Germany Trinationales Institut für Pflanzenforschung, Institut de Biologie Moléculaire des Plantes du CNRS, IBMP-CNRS, Strasbourg Cedex, France arp.schnittger@uni-hamburg.de.
authors: Arp
authors: 0000-0001-7067-0091
authors: A
authors: Schnittger
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D051135:Rad51 Recombinase / Q000378:metabolism; D059767:Recombinational DNA Repair; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C462528:CycB1 protein, Arabidopsis; D019926:Cyclin B; C561308:SOG1 protein, Arabidopsis; D014157:Transcription Factors; C434472:CDKA1 protein, Arabidopsis; C435712:CDKB1;2 protein, Arabidopsis; D018844:Cyclin-Dependent Kinases; C495146:ATRAD51 protein, Arabidopsis; D051135:Rad51 Recombinase
keywords: 
  CDK
  ; DNA damage; cell cycle; cyclin; homologous recombination