title: Herbivorax saccincola gen. nov., sp. nov., a cellulolytic, anaerobic, thermophilic bacterium isolated via in sacco enrichments from a lab-scale biogas reactor.
abstract: A novel Gram-stain-positive, rod-shaped, anaerobic, thermophilic bacterium, strain GGR1T, was isolated from a thermophilic lab-scale biogas fermenter. The novel organism was effectively degrading crystalline cellulose. It seems to play a role in remineralization of plant biomass by hydrolysing its polysaccharides. 16S rRNA gene comparative sequence analysis demonstrated that the isolate formed a hitherto unknown subline within the family Ruminococcaceae. The closest phylogenetic relative of GGR1T among the taxa with validly published names was Clostridiumthermocellum, sharing 94.3 % 16S rRNA gene sequence similarity. Strain GGR1T was catalase-negative, indole-negative and produced acetate and ethanol as major end-products during fermentative cellulose utilization. The major cellular fatty acids (>1 %) were 16 : 0 iso fatty acid and 16 : 0 fatty acid. Cells were rod shaped and grew optimally at 60 °C and pH 7.0. The DNA G+C content was 34.9 mol%. A novel genus and species, Herbivoraxsaccincola gen. nov., sp. nov., is proposed on the basis of phylogenetic analysis and physiological properties of the novel isolate. Strain GGR1T (=DSM 101079T=CECT 9155T) represents the type strain for the novel genus and novel species Herbivoraxsaccincola gen. nov., sp. nov.
authors: Department of Microbiology, Technische Universität München, Emil-Ramann-Str. 4, D-85354 Freising-Weihenstephan, Germany.
authors: Daniela E
authors: DE
authors: Koeck
authors: Department of Microbiology, Technische Universität München, Emil-Ramann-Str. 4, D-85354 Freising-Weihenstephan, Germany.
authors: Matthias
authors: M
authors: Mechelke
authors: Department of Microbiology, Technische Universität München, Emil-Ramann-Str. 4, D-85354 Freising-Weihenstephan, Germany.|Institute of Molecular Genetics, Russian Academy of Science, Kurchatov Sq. 2, 123182 Moscow, Russia.
authors: Vladimir V
authors: VV
authors: Zverlov
authors: Department of Microbiology, Technische Universität München, Emil-Ramann-Str. 4, D-85354 Freising-Weihenstephan, Germany.
authors: Wolfgang
authors: W
authors: Liebl
authors: Department of Microbiology, Technische Universität München, Emil-Ramann-Str. 4, D-85354 Freising-Weihenstephan, Germany.
authors: Wolfgang H
authors: WH
authors: Schwarz
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D056804:Biofuels / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology; D002482:Cellulose / Q000378:metabolism; D000068016:Eubacteriales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005858:Germany; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D056804:Biofuels; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D002482:Cellulose