title: NGS tools for traceability in candies as high processed food products: Ion Torrent PGM versus conventional PCR-cloning.
abstract: The Next Generation Sequencing methodologies are considered the next step within DNA-based methods and their applicability in different fields is being evaluated. Here, we tested the usefulness of the Ion Torrent Personal Genome Machine (PGM) in food traceability analyzing candies as a model of high processed foods, and compared the results with those obtained by PCR-cloning-sequencing (PCR-CS). The majority of samples exhibited consistency between methodologies, yielding more information and species per product from the PGM platform than PCR-CS. Significantly higher AT-content in sequences of the same species was also obtained from PGM. This together with some taxonomical discrepancies between methodologies suggest that the PGM platform is still pre-mature for its use in food traceability of complex highly processed products. It could be a good option for analysis of less complex food, saving time and cost per sample.
authors: Laboratory of Genetics of Natural Resources, Area of Genetics, Department of Functional Biology, University of Oviedo, Asturias, Spain. Electronic address: a.martam.colmenero@gmail.com.
authors: Marta
authors: M
authors: Muñoz-Colmenero
authors: Sequencing Unit of the University of Oviedo, Asturias, Spain. Electronic address: secuenciacion@spi.uniovi.es.
authors: Jose Luis
authors: JL
authors: Martínez
authors: Laboratory of Genetics of Natural Resources, Area of Genetics, Department of Functional Biology, University of Oviedo, Asturias, Spain. Electronic address: aroca@uniovi.es.
authors: Agustín
authors: A
authors: Roca
authors: Laboratory of Genetics of Natural Resources, Area of Genetics, Department of Functional Biology, University of Oviedo, Asturias, Spain. Electronic address: egv@uniovi.es.
authors: Eva
authors: E
authors: Garcia-Vazquez
mesh_terms: D002182:Candy / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
keywords: Candy; Next-Generation-Sequencing; PCR-cloning; PGM Ion Torrent; Traceability