title: Physicochemical characterization of ferumoxytol, heparin and protamine nanocomplexes for improved magnetic labeling of stem cells.
abstract: Stem cell-based therapies have become a major focus in regenerative medicine and to treat diseases. A straightforward approach combining three drugs, heparin (H), protamine (P) with ferumoxytol (F) in the form of nanocomplexes (NCs) effectively labeled stem cells for cellular MRI. We report on the physicochemical characteristics for optimizing the H, P, and F components in different ratios, and mixing sequences, producing NCs that varied in hydrodynamic size. NC size depended on the order in which drugs were mixed in media. Electron microscopy of HPF or FHP showed that F was located on the surface of spheroidal shaped HP complexes. Human stem cells incubated with FHP NCs resulted in a significantly greater iron concentration per cell compared to that found in HPF NCs with the same concentration of F. These results indicate that FHP could be useful for labeling stem cells in translational studies in the clinic.
authors: Laboratory of Diagnostic Radiology Research, Radiology and Imaging Sciences, Clinical Center, Bethesda, MD, USA. Electronic address: HBryant@cc.nih.gov.
authors: L Henry
authors: LH
authors: Bryant
authors: Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
authors: Saejeong J
authors: SJ
authors: Kim
authors: Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
authors: Matthew
authors: M
authors: Hobson
authors: Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
authors: Blerta
authors: B
authors: Milo
authors: Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
authors: Zsofia I
authors: ZI
authors: Kovacs
authors: Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
authors: Neekita
authors: N
authors: Jikaria
authors: Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
authors: Bobbi K
authors: BK
authors: Lewis
authors: Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
authors: Maria A
authors: MA
authors: Aronova
authors: Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA; Department of Biochemistry, Federal University of Sao Paulo, Sao Paulo, Brazil.
authors: Alioscka A
authors: AA
authors: Sousa
authors: Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
authors: Guofeng
authors: G
authors: Zhang
authors: Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
authors: Richard D
authors: RD
authors: Leapman
authors: Laboratory of Diagnostic Radiology Research, Radiology and Imaging Sciences, Clinical Center, Bethesda, MD, USA; Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA; National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. Electronic address: jfrank@helix.nih.gov.
authors: Joseph A
authors: JA
authors: Frank
mesh_terms: D058948:Cell Tracking; D052203:Ferrosoferric Oxide; D006493:Heparin; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D053758:Nanoparticles; D011479:Protamines; D033581:Stem Cell Transplantation; D013234:Stem Cells
chemical_list: D011479:Protamines; D006493:Heparin; D052203:Ferrosoferric Oxide
keywords: Cell labeling; Ferumoxytol; Heparin; Protamine; Stem cells