title: Large-Conductance Transmembrane Porin Made from DNA Origami.
abstract: DNA nanotechnology allows for the creation of three-dimensional structures at nanometer scale. Here, we use DNA to build the largest synthetic pore in a lipid membrane to date, approaching the dimensions of the nuclear pore complex and increasing the pore-area and the conductance 10-fold compared to previous man-made channels. In our design, 19 cholesterol tags anchor a megadalton funnel-shaped DNA origami porin in a lipid bilayer membrane. Confocal imaging and ionic current recordings reveal spontaneous insertion of the DNA porin into the lipid membrane, creating a transmembrane pore of tens of nanosiemens conductance. All-atom molecular dynamics simulations characterize the conductance mechanism at the atomic level and independently confirm the DNA porins' large ionic conductance.
authors: Cavendish Laboratory, University of Cambridge , Cambridge CB3 0HE, United Kingdom.
authors: Kerstin
authors: K
authors: Göpfrich
authors: Center for the Physics of Living Cells, Department of Physics, University of Illinois at Urbana-Champaign , 1110 West Green Street, Urbana, Illinois 61801, United States.
authors: Chen-Yu
authors: CY
authors: Li
authors: Cavendish Laboratory, University of Cambridge , Cambridge CB3 0HE, United Kingdom.
authors: Maria
authors: M
authors: Ricci
authors: Jacobs University Bremen , 28759 Bremen, Germany.
authors: Satya Prathyusha
authors: SP
authors: Bhamidimarri
authors: Center for the Physics of Living Cells, Department of Physics, University of Illinois at Urbana-Champaign , 1110 West Green Street, Urbana, Illinois 61801, United States.
authors: Jejoong
authors: J
authors: Yoo
authors: Cavendish Laboratory, University of Cambridge , Cambridge CB3 0HE, United Kingdom.
authors: Bertalan
authors: B
authors: Gyenes
authors: Cavendish Laboratory, University of Cambridge , Cambridge CB3 0HE, United Kingdom.
authors: Alexander
authors: A
authors: Ohmann
authors: Jacobs University Bremen , 28759 Bremen, Germany.
authors: Mathias
authors: M
authors: Winterhalter
authors: Center for the Physics of Living Cells, Department of Physics, University of Illinois at Urbana-Champaign , 1110 West Green Street, Urbana, Illinois 61801, United States.
authors: Aleksei
authors: A
authors: Aksimentiev
authors: Cavendish Laboratory, University of Cambridge , Cambridge CB3 0HE, United Kingdom.
authors: Ulrich F
authors: UF
authors: Keyser
mesh_terms: D004247:DNA; D008051:Lipid Bilayers; D056004:Molecular Dynamics Simulation; D036103:Nanotechnology; D018272:Porins
chemical_list: D008051:Lipid Bilayers; D018272:Porins; D004247:DNA
keywords: DNA origami; ionic current recordings; lipid membrane; molecular dynamics; synthetic porin