title: Quillaja saponin: A prospective emulsifier for the preparation of solid lipid nanoparticles.
abstract: Quillaja saponin (QS) is a non-ionic amphiphilic surfactant of natural origin. In the present study, we evaluated its potential to form solid lipid nanoparticles (SLNs) in the presence of stearic acid (SA) as a lipid carrier and imatinib mesylate (IM) as a model drug. IM loaded solid lipid nanoparticles (IMSLNs) were prepared using the hot homogenisation method. Characterisation of IMSLNs revealed that they were quasi-spherical in shape, neutrally charged and 143.5-641.9nm in size. Haemolysis, a toxicity issue of QS was not observed in SLNs. Comparative in vitro cytotoxicity analyses performed in human breast cancer cell line MCF7 revealed that IMSLNs were more toxic than IM. On the other hand, in vitro viability studies in the RAW264.7 cell line did not show any sign of toxicity by IMSLNs. Our results indicate that QS hold great potential in nano drug delivery as an emulsifier.
authors: Department of Pharmaceutics, PSG College of Pharmacy, Peelamedu, Coimbatore, 641004, India.
authors: Siram
authors: S
authors: Karthik
authors: Department of Pharmaceutics, PSG College of Pharmacy, Peelamedu, Coimbatore, 641004, India.
authors: Chellan Vijaya
authors: CV
authors: Raghavan
authors: Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB), University of Minho, Braga 4710-057, Portugal.
authors: Gregory
authors: G
authors: Marslin
authors: Department of Pharmaceutics, PSG College of Pharmacy, Peelamedu, Coimbatore, 641004, India.
authors: Habibur
authors: H
authors: Rahman
authors: Department of Pharmacology, PSG College of Pharmacy, Peelamedu Coimbatore, 641004, India.
authors: Divakar
authors: D
authors: Selvaraj
authors: Department of Pharmaceutics, PSG College of Pharmacy, Peelamedu, Coimbatore, 641004, India.
authors: Krishnamoorthy
authors: K
authors: Balakumar
authors: Department of Integrative Plant Biology, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland. Electronic address: fgre@igr.poznan.pl.
authors: Gregory
authors: G
authors: Franklin
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D045703:Emulsifying Agents / Q000737:chemistry*; D006801:Humans; D000068877:Imatinib Mesylate / Q000737:chemistry / Q000494:pharmacology; D061986:MCF-7 Cells; D008264:Macrophages / Q000166:cytology / Q000187:drug effects*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D062605:Quillaja Saponins / Q000737:chemistry*; D013229:Stearic Acids / Q000737:chemistry*; D013501:Surface-Active Agents
chemical_list: D004337:Drug Carriers; D045703:Emulsifying Agents; D062605:Quillaja Saponins; D013229:Stearic Acids; D013501:Surface-Active Agents; C031183:stearic acid; D000068877:Imatinib Mesylate
keywords: Haemolytic test; Imatinib mesylate; Quillaja saponin; Solid lipid nanoparticles; Stearic acid