title: Evaluation of the Role of the LysM Receptor-Like Kinase, OsNFR5/OsRLK2 for AM Symbiosis in Rice.
abstract: In legume-specific rhizobial symbiosis, host plants perceive rhizobial signal molecules, Nod factors, by a pair of LysM receptor-like kinases, NFR1/LYK3 and NFR5/NFP, and activate symbiotic responses through the downstream signaling components also required for arbuscular mycorrhizal (AM) symbiosis. Recently, the rice NFR1/LYK3 ortholog, OsCERK1, was shown to play crucial roles for AM symbiosis. On the other hand, the roles of the NFR5/NFP ortholog in rice have not been elucidated, while it has been shown that NFR5/NFP orthologs, Parasponia PaNFR5 and tomato SlRLK10, engage in AM symbiosis. OsCERK1 also triggers immune responses in combination with a receptor partner, OsCEBiP, against fungal or bacterial infection, thus regulating opposite responses against symbiotic and pathogenic microbes. However, it has not been elucidated how OsCERK1 switches these opposite functions. Here, we analyzed the function of the rice NFR5/NFP ortholog, OsNFR5/OsRLK2, as a possible candidate of the OsCERK1 partner for symbiotic signaling. Inoculation of AM fungi induced the expression of OsNFR5 in the rice root, and the chimeric receptor consisting of the extracellular domain of LjNFR5 and the intracellular domain of OsNFR5 complemented the Ljnfr5 mutant for rhizobial symbiosis, indicating that the intracellular kinase domain of OsNFR5 could activate symbiotic signaling in Lotus japonicus. Although these data suggested the possible involvement of OsNFR5 in AM symbiosis, osnfr5 knockout mutants were colonized by AM fungi similar to the wild-type rice. These observations suggested several possibilities including the presence of functionally redundant genes other than OsNFR5 or involvement of novel ligands, which do not require OsNFR5 for recognition.
authors: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Kanagawa, 214-8571 Japan.
authors: Kana
authors: K
authors: Miyata
authors: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Kanagawa, 214-8571 Japan.
authors: Masahiro
authors: M
authors: Hayafune
authors: National Agriculture and Food Research Organization (NARO), Hokkaido Agricultural Research Center, Agro-environmental Research Division 1, Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555 Japan.
authors: Yoshihiro
authors: Y
authors: Kobae
authors: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Kanagawa, 214-8571 Japan.
authors: Hanae
authors: H
authors: Kaku
authors: Division of Plant and Microbial Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, 305-8602 Japan.
authors: Yoko
authors: Y
authors: Nishizawa
authors: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Kanagawa, 214-8571 Japan.
authors: Yoshiki
authors: Y
authors: Masuda
authors: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Kanagawa, 214-8571 Japan nkgwtmm@nibb.ac.jp shibuya@meiji.ac.jp.
authors: Naoto
authors: N
authors: Shibuya
authors: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Kanagawa, 214-8571 Japan nkgwtmm@nibb.ac.jp shibuya@meiji.ac.jp.|Division of Symbiotic Systems, National Institute for Basic Biology, Nishigonaka 38, Myodaiji, Okazaki, Aichi, 444-8585 Japan.|Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Aichi, 464-8602 Japan.
authors: Tomomi
authors: T
authors: Nakagawa
mesh_terms: D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D017343:Genes, Plant; D005816:Genetic Complementation Test; D000070116:Lotus / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D038821:Mycorrhizae / Q000502:physiology*; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D055170:Plant Root Nodulation / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D055503:Protein Multimerization; D017386:Sequence Homology, Amino Acid; D013559:Symbiosis / Q000235:genetics
chemical_list: D010940:Plant Proteins; D011494:Protein Kinases
keywords: Arbuscular mycorrhizal symbiosis; LysM receptor-like kinase; OsCERK1; OsNFR5; Rhizobial symbiosis; Rice (Oryza sativa)