title: First evidence on the occurrence and dynamics of Dehalococcoides mccartyi PCB-dechlorinase genes in marine sediment during Aroclor1254 reductive dechlorination.
abstract: The present study evaluates the PCB-dehalorespiring capabilities and dynamics of indigenous Dehalococcoides mccartyi population in a PCB contaminated marine sediment. Specialized PCB-dechlorinase genes pcbA1, pcbA4 and pcbA5 previously characterized in pure cultures of D. mccartyi, were here found for the first time in environmental samples. Reductive dechlorination was stimulated by spiking Aroclor1254 to the sediment and by imposing strictly anaerobic conditions both with and without bioaugmentation with a Dehalococcoides mccartyi enrichment culture. In line with the contaminant dechlorination kinetics, Dehalococcoides population increased during the entire incubation period showing growth yields of 4.94E+07 Dehalococcoides per μmolCl-1and 7.30E+05 Dehalococcoides per μmolCl-1in the marine sediment with and without bioaugmentation respectively. The pcbA4 and pcbA5 dechlorinase genes, and to a lesser extent pcbA1 gene, were enriched during the anaerobic incubation suggesting their role in Aroclor1254 dechlorination under salinity conditions.
authors: Water Research Institute, IRSA-CNR, Via Salaria km 29,300, Monterotondo (RM), Italy.
authors: B
authors: B
authors: Matturro
authors: Water Research Institute, IRSA-CNR, Via Salaria km 29,300, Monterotondo (RM), Italy.
authors: M
authors: M
authors: Di Lenola
authors: ENEA, Technical Unit for Environmental Characterization, Prevention and Remediation, UTPRA, C. R. Casaccia, Italy.
authors: C
authors: C
authors: Ubaldi
authors: Water Research Institute, IRSA-CNR, Via Salaria km 29,300, Monterotondo (RM), Italy. Electronic address: rossetti@irsa.cnr.it.
authors: S
authors: S
authors: Rossetti
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D020111:Chlorodiphenyl (54% Chlorine) / Q000737:chemistry / Q000378:metabolism*; D041862:Chloroflexi / Q000235:genetics* / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D054879:Halogenation; D059013:Microbial Consortia / Q000235:genetics; D011078:Polychlorinated Biphenyls / Q000737:chemistry / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D014874:Water Pollutants, Chemical; D020111:Chlorodiphenyl (54% Chlorine); D011078:Polychlorinated Biphenyls
keywords: Aroclor1254; Dehalococcoides mccartyi; Marine sediment; PCB-dechlorinase genes; Polychlorinated biphenyls; Reductive dechlorination