title: Investigation of the association between miR‑181b, Bcl‑2 and LRIG1 in oral verrucous carcinoma.
abstract: Abnormal expression of microRNAs (miRNAs) is involved in the development of and anti‑apoptotic effects in various types of human cancer. However, miRNA‑mediated regulation of oral verrucous carcinoma (OVC) remains to be elucidated. The present study aimed to investigate the expression of miR‑181b in OVC and oral squamous cell carcinoma (OSCC). The expression levels of miR‑181b were determined using reverse transcription‑quantitative polymerase chain reaction. The expression levels of B‑cell lymphoma 2 (Bcl‑2) and leucine rich repeats and immunoglobulin like domains 1 (LRIG1), were evaluated using immunohistochemical staining. The correlation between Bcl‑2 and LRIG1 expression was determined using a Pearson correlation analysis. The expression levels of miR‑181b and Bcl‑2 in OVC were significantly higher compared with normal mucosal tissue (NM); however, lower compared with the OSCC. The key target of miR‑181b was LRIG1 and it was significantly lower in OVC tissues compared with NM tissue; however this was higher when compared with OSCC tissue. The expression levels of Bcl‑2 were correlated with expression levels of LRIG1 in OVC tissues. Therefore, LRIG1 may be associated with anti‑apoptotic function in OVC tissues.
authors: Department of Oral and Maxillofacial Surgery, Xiangya School and Hospital of Stomatology, Central South University, Changsha, Hunan 410078, P.R. China.
authors: Zhi-Yuan
authors: ZY
authors: Deng
authors: Department of Oral and Maxillofacial Surgery, Xiangya School and Hospital of Stomatology, Central South University, Changsha, Hunan 410078, P.R. China.
authors: Yue-Hong
authors: YH
authors: Wang
authors: Department of Oral and Maxillofacial Surgery, Xiangya School and Hospital of Stomatology, Central South University, Changsha, Hunan 410078, P.R. China.
authors: Hong-Zhi
authors: HZ
authors: Quan
authors: Department of Oral and Maxillofacial Surgery, Xiangya School and Hospital of Stomatology, Central South University, Changsha, Hunan 410078, P.R. China.
authors: Ou-Sheng
authors: OS
authors: Liu
authors: Department of Prosthodontics, Xiangya School and Hospital of Stomatology, Central South University, Changsha, Hunan 410078, P.R. China.
authors: Yi-Ping
authors: YP
authors: Li
authors: Department of Oral and Maxillofacial Surgery, Changsha Stomatological Hospital, Changsha, Hunan 410005, P.R. China.
authors: Yuan
authors: Y
authors: Li
authors: Department of Oral and Maxillofacial Surgery, Yiyang Central Hospital, Yiyang, Hunan 413000, P.R. China.
authors: Wu
authors: W
authors: Zhu
authors: Department of Oral and Maxillofacial Surgery, Xiangya School and Hospital of Stomatology, Central South University, Changsha, Hunan 410078, P.R. China.
authors: Krishna
authors: K
authors: Munnee
authors: Department of Oral and Maxillofacial Surgery, Xiangya School and Hospital of Stomatology, Central South University, Changsha, Hunan 410078, P.R. China.
authors: Zhan-Gui
authors: ZG
authors: Tang
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D018289:Carcinoma, Verrucous / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009055:Mouth / Q000378:metabolism / Q000473:pathology; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000235:genetics*; D015854:Up-Regulation; D055815:Young Adult
chemical_list: C484445:LRIG1 protein, human; C000608460:MIRN-181 microRNA, human; D008562:Membrane Glycoproteins; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2