title: Core-Shell Silver/Polymeric Nanoparticles-Based Combinatorial Therapy against Breast Cancer In-vitro.
abstract: The current study aimed at preparing AgNPs and three different core-shell silver/polymeric NPs composed of Ag core and three different polymeric shells: polyvinyl alcohol (PVA), polyethylene glycol (PEG) and polyvinylpyrrolidone (PVP). Thereafter, the core/shell NPs were loaded with a chemotherapeutic agent doxorubicin (DOX). Finally, the cytotoxic effects of the different core-shell Ag/polymeric NPs-based combinatorial therapeutics were tested in-vitro against breast cancer (MCF-7) and human fibroblast (1BR hTERT) cell lines. AgNPs, Ag/PVA and Ag/PVP NPs were more cytotoxic to MCF-7 cells than normal fibroblasts, as well as DOX-Ag, DOX-Ag/PVA, DOX-Ag/PEG and DOX-Ag/PVP nanocarriers (NCs). Notably, low dosage of core-shell DOX-loaded Ag/polymeric nanocarriers (NCs) exhibited a synergic anticancer activity, with DOX-Ag/PVP being the most cytotoxic. We believe that the prepared NPs-based combinatorial therapy showed a significant enhanced cytotoxic effect against breast cancer cells. Future studies on NPs-based combinatorial therapy may aid in formulating a novel and more effective cancer therapeutics.
authors: Yousef Jameel Science and Technology Research Center (YJSTRC), School of Sciences and Engineering, American University in Cairo, 11835, Egypt.|Department of Chemistry, School of Sciences and Engineering, American University in Cairo, 11835, Egypt.
authors: Nancy M
authors: NM
authors: Elbaz
authors: Yousef Jameel Science and Technology Research Center (YJSTRC), School of Sciences and Engineering, American University in Cairo, 11835, Egypt.|Department of Biology and Biotechnology Graduate Program, School of Sciences and Engineering, American University in Cairo, 11835, Egypt.
authors: Laila
authors: L
authors: Ziko
authors: Yousef Jameel Science and Technology Research Center (YJSTRC), School of Sciences and Engineering, American University in Cairo, 11835, Egypt.|Department of Biology and Biotechnology Graduate Program, School of Sciences and Engineering, American University in Cairo, 11835, Egypt.
authors: Rania
authors: R
authors: Siam
authors: Yousef Jameel Science and Technology Research Center (YJSTRC), School of Sciences and Engineering, American University in Cairo, 11835, Egypt.|Department of Chemistry, School of Sciences and Engineering, American University in Cairo, 11835, Egypt.
authors: Wael
authors: W
authors: Mamdouh
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D004359:Drug Therapy, Combination; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D061986:MCF-7 Cells; D057145:Nanoshells / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011205:Povidone / Q000737:chemistry; D012834:Silver / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D011108:Polymers; D012834:Silver; D011092:Polyethylene Glycols; D004317:Doxorubicin; D011142:Polyvinyl Alcohol; D011205:Povidone