title: Performance of self-collected penile-meatal swabs compared to clinician-collected urethral swabs for the detection of Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, and Mycoplasma genitalium by nucleic acid amplification assays.
abstract: Men were enrolled in a study to assess the performance and acceptability of self-collected penile meatal swabs as compared to clinician-collected urethral swabs for sexually transmitted infections (STIs). We expected penile-meatal swabs to perform favorably to urethral swabs for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV), and Mycoplasma genitalium (MG) detection by nucleic acid amplification assays (NAATs). Of 203 swab pairs tested; for CT, penile-meatal swab sensitivity was 96.8% and specificity was 98.8%. NG sensitivity and specificity were 100% and 98.9%, respectively. For TV, sensitivity was 85.0% and specificity was 96.7%. For MG sensitivity and specificity were 79.3% and 99.4%, respectively. No significant statistical differences between sample type accuracy (CT: P=0.625; NG: P=0.248; TV: P=0.344; and MG: P=0.070) existed. Most men, 90.1%, reported self-collection of penile-meatal swabs as "Very Easy" or "Easy". Self-collected penile-meatal swabs appeared acceptable for NAAT STI detection and an acceptable collection method by men.
authors: Johns Hopkins University, Division of Infectious Diseases, Baltimore, MD. Electronic address: Ldize2@jhmi.edu.
authors: Laura
authors: L
authors: Dize
authors: Johns Hopkins University, Division of Infectious Diseases, Baltimore, MD.
authors: Perry
authors: P
authors: Barnes
authors: Johns Hopkins University, Division of Infectious Diseases, Baltimore, MD.
authors: Mathilda
authors: M
authors: Barnes
authors: Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD.
authors: Yu-Hsiang
authors: YH
authors: Hsieh
authors: Baltimore City Health Department, Baltimore, MD.
authors: Vincent
authors: V
authors: Marsiglia
authors: Baltimore City Health Department, Baltimore, MD.
authors: Della
authors: D
authors: Duncan
authors: Johns Hopkins University, Division of Infectious Diseases, Baltimore, MD.
authors: Justin
authors: J
authors: Hardick
authors: Johns Hopkins University, Division of Infectious Diseases, Baltimore, MD; Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD.
authors: Charlotte A
authors: CA
authors: Gaydos
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D045704:Mycoplasma genitalium / Q000235:genetics / Q000302:isolation & purification; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques; D010413:Penis / Q000382:microbiology* / Q000469:parasitology*; D012646:Self Administration / Q000379:methods*; D012680:Sensitivity and Specificity; D012749:Sexually Transmitted Diseases / Q000175:diagnosis*; D013048:Specimen Handling / Q000379:methods*; D014246:Trichomonas vaginalis / Q000235:genetics / Q000302:isolation & purification; D055815:Young Adult
keywords: Acceptability; Chlamydia trachomatis; Mycoplasma genitalium; Neisseria gonorrhoeae; Penile-meatal swabs; Self-collection; Trichomonas vaginalis