title: Thermoresponsive diblock glycopolymer by RAFT polymerization for lectin recognition.
abstract: A thermoresponsive double-hydrophilic diblock glycopolymer, poly(diethyl- eneglycol methacrylate)-block-poly(6-O-vinyladipoyl-d-glucose) (PDEGMA-b-POVAG), was successfully prepared by a combination of enzymatic synthesis and reversible addition-fragment chain transfer (RAFT) polymerization protocols using poly(diethyl- eneglycol methacrylate) (PDEGMA) as macro-RAFT agent. The block glycopolymer was characterized by (1)H NMR and GPC. UV-vis, DLS and TEM studies revealed that the glycopolymer PDEGMA-b-POVAG was thermoresponsive with LCST at 31.0°C, and was able to self-assemble into spherical micelles of various sizes in aqueous solution. The glucose pendants in the glycopolymer could interact with the lectin Concanavalin A (Con A), the average hydrodynamic diameters of glycopolymer micelles increased to 170nm from 110nm after recognizing Con A. The diblock glycopolymer micelles have excellent biocompatibility with pig iliac endothelial cells, as measured using the MTT assay, but micelles loaded with Con A could be used to induce apoptosis in human hepatoma SMMC-7721 cells.
authors: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors: Kan
authors: K
authors: Sun
authors: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors: Muru
authors: M
authors: Xu
authors: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors: Kaichun
authors: K
authors: Zhou
authors: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors: Huali
authors: H
authors: Nie
authors: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China; Key Lab of Eco-Textile, Ministry of Education, Donghua University, PR China. Electronic address: jquan@dhu.edu.cn.
authors: Jing
authors: J
authors: Quan
authors: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: lzhu@dhu.edu.cn.
authors: Limin
authors: L
authors: Zhu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003208:Concanavalin A / Q000737:chemistry*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007082:Ileum / Q000166:cytology / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D013552:Swine
chemical_list: D003208:Concanavalin A; D011092:Polyethylene Glycols; D005947:Glucose
keywords: Biocompatibility; Double-hydrophilic; Lectin recognition; RAFT; Thermosensitive