title: Overview of online two-dimensional liquid chromatography based on cell membrane chromatography for screening target components from traditional Chinese medicines.
abstract: Cell membrane chromatography is a simple, specific, and time-saving technique for studying drug-receptor interactions, screening of active components from complex mixtures, and quality control of traditional Chinese medicines. However, the short column life, low sensitivity, low column efficiency (so cannot resolve satisfactorily mixture of compounds), low peak capacity, and inefficient in structure identification were bottleneck in its application. Combinations of cell membrane chromatography with multidimensional chromatography such as two-dimensional liquid chromatography and high sensitivity detectors like mass have significantly reduced many of the above-mentioned shortcomings. This paper provides an overview of the current advances in online two-dimensional-based cell membrane chromatography for screening target components from traditional Chinese medicines with particular emphasis on the instrumentation, preparation of cell membrane stationary phase, advantages, and disadvantages compared to alternative approaches. The last section of the review summarizes the applications of the online two-dimensional high-performance liquid chromatography based cell membrane chromatography reported since its emergence to date (2010-June 2016).
authors: School of Pharmacy, Xi'an Jiaotong University, Shaanxi, China.
authors: Saqib
authors: S
authors: Muhammad
authors: School of Pharmacy, Xi'an Jiaotong University, Shaanxi, China.
authors: Shengli
authors: S
authors: Han
authors: School of Pharmacy, Xi'an Jiaotong University, Shaanxi, China.
authors: Xiaoyu
authors: X
authors: Xie
authors: School of Pharmacy, Xi'an Jiaotong University, Shaanxi, China.
authors: Sicen
authors: S
authors: Wang
authors: School of Pharmacy, Xi'an Jiaotong University, Shaanxi, China.
authors: Muhammad Majid
authors: MM
authors: Aziz
mesh_terms: D002462:Cell Membrane / Q000737:chemistry; D002853:Chromatography, Liquid / Q000592:standards*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Cell membrane chromatography; Mass spectrometry; Online two-dimensional liquid chromatography; Traditional Chinese medicines