title: Fluorescence labels may significantly affect the protein adsorption on hydrophilic nanomaterials.
abstract: Fluorescently labelled proteins are often used to study processes in vitro, e.g. the binding of proteins to cell surfaces or the adsorption of plasma proteins on drug nanocarriers. However, the fact that the fluorescent labelling may affect the protein properties is frequently neglected. On the example of a simple model system, we reiterate the importance of this issue by showing that even a single label may perturb interactions between hydrophilic starch-based nanocapsules and serum albumin and thus prevent binding.
authors: Max Planck Institute for Polymer Research, Ackermannweg 10, D-55128 Mainz, Germany. Electronic address: winzen@mpip-mainz.mpg.de.
authors: Svenja
authors: S
authors: Winzen
authors: Max Planck Institute for Polymer Research, Ackermannweg 10, D-55128 Mainz, Germany. Electronic address: koynov@mpip-mainz.mpg.de.
authors: Kaloian
authors: K
authors: Koynov
authors: Max Planck Institute for Polymer Research, Ackermannweg 10, D-55128 Mainz, Germany. Electronic address: landfester@mpip-mainz.mpg.de.
authors: Katharina
authors: K
authors: Landfester
authors: Max Planck Institute for Polymer Research, Ackermannweg 10, D-55128 Mainz, Germany. Electronic address: krisgabr@gmail.com.
authors: Kristin
authors: K
authors: Mohr
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005456:Fluorescent Dyes; D011108:Polymers; D012709:Serum Albumin
keywords: Fluorescence correlation spectroscopy; Fluorescence label; Isothermal titration calorimetry; Nanoparticles; Protein adsorption