title: Simultaneous quantification of triterpenoic acids by high performance liquid chromatography method in the extracts of gum resin of Boswellia serrata obtained by different extraction techniques.
abstract: BACKGROUND
  Boswellia serrata, also known as Indian frankincense is a commercially important medicinal plant which has been used for hundreds of years as an Ayurvedic medicine for the attempted treatment of arthritis. It contains naturally occurring triterpenoic acids, called as boswellic acids (BA's).
  
  
  RESULTS
  A highly reproducible High performance liquid chromatography-ultraviolet diode array detection (HPLC-UV-DAD) method was developed for the simultaneous determination and quantitative analysis of eight major triterpenoic acids in Boswellia serrata gum resin obtained by different extraction techniques. All the calibration curves exhibited good linear regression (R(2) > 0.997) within the test ranges. The established method showed good precision and overall recoveries of the boswellic acids.
  
  
  CONCLUSIONS
  The eight triterpenoic acids coded as BS-1 (11-keto-beta-boswellic acid), BS-2 (3-O-acetyl-11-keto-beta-boswellic acid), BS-3 (3-keto tirucallic acid), BS-4 (3-O-acetyl-alpha-tirucallic acid), BS-5 (3-O-acetyl-beta-tirucallic acid), BS-6 (alpha-boswellic acid), BS-7 (beta-boswellic acid) and BS-8 (3-O-acetyl-beta-boswellic acid) were isolated from the processed gum resin of Boswellia serrata by column chromatography. The proposed HPLC method is simple, reliable and has been very useful for the qualitative as well as quantitative analysis of boswellic acids in the gum resin of Boswellia serrata. The proposed method allows to quantify boswellic acids in appreciable amounts by HPLC-UV (DAD) method in the extracts and the available marketed formulations.Graphical abstractIsolation & separation of eight Triterpenoic acids from Boswellia serrata.
authors: Analytical Chemistry Division (Instrumentation), CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001 Jammu and Kashmir India ; Natural Product Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001 India.
authors: Neha
authors: N
authors: Sharma
authors: Analytical Chemistry Division (Instrumentation), CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001 Jammu and Kashmir India.
authors: Vikram
authors: V
authors: Bhardwaj
authors: Bio-organic Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001 India.
authors: Samar
authors: S
authors: Singh
authors: Department of Biotechnology, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001 India.
authors: Sheikh Abid
authors: SA
authors: Ali
authors: Bio-organic Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001 India.
authors: D K
authors: DK
authors: Gupta
authors: Department of Chemistry, University of Jammu, Jammu, 180006 India.
authors: Satya
authors: S
authors: Paul
authors: Natural Product Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001 India.
authors: Naresh K
authors: NK
authors: Satti
authors: Genetic Resource and Agrotech, Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001 India.
authors: Suresh
authors: S
authors: Chandra
authors: Analytical Chemistry Division (Instrumentation), CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001 Jammu and Kashmir India.
authors: Mahendra K
authors: MK
authors: Verma
keywords: Boswellia seratta; ESI–MS (electrospray ionisation mass spectrometry); HPLC; Triterpenoic acids; UV (DAD)