title: Characterization and use of new monoclonal antibodies to CD11c, CD14, and CD163 to analyze the phenotypic complexity of ruminant monocyte subsets.
abstract: The sequencing of the bovine genome and development of mass spectrometry, in conjunction with flow cytometry (FC), have afforded an opportunity to complete the characterization of the specificity of monoclonal antibodies (mAbs), only partially characterized during previous international workshops focused on antibody development for livestock (1991, Leukocyte Antigens in Cattle, Sheep, and Goats; 1993, Leukocyte Antigens of Cattle and Sheep; 1996, Third Workshop on Ruminant Leukocyte Antigens). The objective of this study was to complete the characterization of twelve mAbs incompletely characterized during the workshops that reacted with molecules predominantly expressed on bovine monocytes and use them to provide further information on the phenotypic complexity of monocyte subsets in ruminants. Analysis revealed that the mAbs could be grouped into three clusters that recognize three different molecules: CD11c, CD14, and CD163. Following characterization, comparison of the patterns of expression of CD14 and CD163 with expression of CD16, CD172a, and CD209 revealed the mononuclear cell population is comprised of multiple subsets with differential expression of these molecules. Further analysis revealed the epitopes recognized by mAbs to CD14 and CD163 are conserved on orthologues in sheep and goats. In contrast to CD14 that is also expressed on sheep and goat granulocytes, CD163 is a definitive marker for their monocytes.
authors: Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA; Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Egypt.
authors: Mahmoud M
authors: MM
authors: Elnaggar
authors: Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA; Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Egypt.
authors: Gaber S
authors: GS
authors: Abdellrazeq
authors: Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA.
authors: Victoria
authors: V
authors: Mack
authors: Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA; Animal Disease Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Pullman, WA, 99164, USA.
authors: Lindsay M
authors: LM
authors: Fry
authors: Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA.
authors: William C
authors: WC
authors: Davis
authors: Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA; Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea. Electronic address: magic007@snu.ac.kr.
authors: Kun Taek
authors: KT
authors: Park
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000276:immunology*; D000918:Antibody Specificity; D015703:Antigens, CD / Q000276:immunology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology*; D039521:CD11c Antigen / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D005434:Flow Cytometry; D006041:Goats; D018950:Lipopolysaccharide Receptors / Q000276:immunology*; D008297:Male; D051379:Mice; D009000:Monocytes / Q000145:classification* / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000276:immunology*; D012418:Ruminants / Q000097:blood* / Q000276:immunology*; D012756:Sheep; D000098902:CD163 Antigen
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D039521:CD11c Antigen; D000098902:CD163 Antigen; D018950:Lipopolysaccharide Receptors; D011956:Receptors, Cell Surface
keywords: CD11c; CD14; CD16; CD163; Macrophages; Monoclonal antibody; Monocytes