title: A new quantitative method for the analysis of monoacylglycerol isomers using(13)C nuclear magnetic resonance spectroscopy.
abstract: Current methods for determining the regiochemistry of monoacylglycerols are lengthy, tedious and aggravated by the ready isomerization of 2-acyl-sn-glycerols. A new method employs a very rapid adsorption chromatography step in which isomerization is kept to a minimum, followed by formation of the isopropylidene derivatives using mild procedures. These cyclic derivatives of 1- and 3-acyl-sn-glycerols and 2-acyl-sn-glycerols are stable thermally and display certain characteristic(13)C NMR resonances. Integration of these resonances yields directly the proportion of monoacylglycerol isomers in the mixture. The method was applied to the analysis of monoacylglycerols produced by enzymatic hydrolysis of synthetic and naturally occurring triacylglycerols.
authors: Atlantic Research Laboratory, National Research Council of Canada, 1411 Oxford St., B3H 3Z1, Halifax, Nova Scotia, Canada.
authors: R D
authors: RD
authors: Dawe
authors: Atlantic Research Laboratory, National Research Council of Canada, 1411 Oxford St., B3H 3Z1, Halifax, Nova Scotia, Canada.
authors: J J
authors: JJ
authors: Wright