title: Antiamyloidogenic Effects of Ellagic Acid on Human Serum Albumin Fibril Formation Induced by Potassium Sorbate and Glucose.
abstract: Oxidative stress has the main role in protein conformational changes and consequent direct involvement in different kind of diseases. Potassium sorbate as a widespread industrial preservative and glucose are two important oxidants that can be involved in oxidative stress. In this study the effect of ellagic acid as a phenolic antioxidant on amyloid fibril formation of human serum albumin upon incubation of potassium sorbate and glucose was studied using thioflavin T assay, surface tension, atomic force microscopy, Amadori product, and carbonyl content assays. The thioflavin T assay and atomic force microscopy micrographs demonstrated the antiamyloidogenic effect of ellagic acid on the human serum albumin fibril formation. This antioxidant also had the repair effect on surface tension of the modified human serum albumin (amyloid intermediates), which was destructed, caused by potassium sorbate and glucose. This mechanism takes place because of potent carbonyl stress suppression effect of ellagic acid, which was strengthening by potassium sorbate in the presence and absence of glucose.
authors: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.|Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran.
authors: F
authors: F
authors: Taghavi
authors: School of Biology, University of Tehran, Tehran, Iran.
authors: M
authors: M
authors: Habibi-Rezaei
authors: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.|School of Science, University of Zabol, Zabol, Iran.
authors: M
authors: M
authors: Bohlooli
authors: ENT-HNS Research Center, IRAN University of Medical Sciences (IUMS), Tehran, Iran.
authors: M
authors: M
authors: Farhadi
authors: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors: M
authors: M
authors: Goodarzi
authors: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors: S
authors: S
authors: Movaghati
authors: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors: P
authors: P
authors: Maghami
authors: Department of Pharmacology, College of Medicine, University of Saskatchewan, Saskatchewan, Canada.
authors: C
authors: C
authors: Taghibiglou
authors: Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: M
authors: M
authors: Amanlou
authors: UR 1268 Biopolymères Interactions Assemblages, INRA, équipe Fonctions et Interactions des Protéines, Nantes, France.|Department of Animal Nutrition and Feed Management, Poznan University of Life Sciences, Poznań, Poland.
authors: T
authors: T
authors: Haertlé
authors: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.|Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran.
authors: A A
authors: AA
authors: Moosavi-Movahedi
mesh_terms: D004610:Ellagic Acid / Q000494:pharmacology*; D005947:Glucose / Q000009:adverse effects; D006031:Glycosylation; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects*; D011487:Protein Conformation; D012709:Serum Albumin / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D013011:Sorbic Acid / Q000009:adverse effects; D013500:Surface Tension / Q000187:drug effects
chemical_list: D012709:Serum Albumin; D004610:Ellagic Acid; D005947:Glucose; D013011:Sorbic Acid
keywords: HSA; ellagic acid; glucose; glycation; oxidative stress; potassium sorbate; reactive carbonyl species