title: Freeze Injury of the Tibialis Anterior Muscle.
abstract: Freeze injury is physically induced by exposing skeletal muscle to an extremely cold probe, and results in a robust degenerative and inflammatory response. One unique aspect of freeze injury is that it destroys not only the muscle fiber cells, but also all of the mononuclear cells in the zone of injury. Repair of the muscle is accomplished by satellite cells from outside of the zone of injury, which must migrate in and which may interact with inflammatory cells, hence the length of time before apparent histological recovery of the most damaged zone is typically somewhat longer with freeze injury than with other physical or chemical methods of injury. In this chapter, we present a detailed protocol for the freeze injury of the tibialis anterior (TA) muscle in mouse.
authors: Department of Physical Medicine and Rehabilitation, University of Minnesota, Minneapolis, MN, 55455, USA.
authors: Gengyun
authors: G
authors: Le
authors: Programs in Physical Therapy and Rehabilitation Science, Department of Physical Medicine and Rehabilitation Medical School, University of Minnesota, Minneapolis, MN, 55455, USA.
authors: Dawn A
authors: DA
authors: Lowe
authors: Lillehei Heart Institute and Department of Pediatrics, University of Minnesota, Cancer and Cardiovascular Research Building 4-127, 2231 6th St. S.E., Minneapolis, MN, 55455, USA. kyba@umn.edu.
authors: Michael
authors: M
authors: Kyba
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005615:Freezing / Q000009:adverse effects*; D051379:Mice; D053580:Muscle Strength; D018482:Muscle, Skeletal / Q000293:injuries* / Q000473:pathology*
keywords: Freeze injury; Regeneration; Tibialis anterior muscle