title: Three dimensional poly(ε-caprolactone) and silk fibroin nanocomposite fibrous matrix for artificial dermis.
abstract: Ideal dermal substitutes should have comparable physicochemical and biological properties to the natural skin tissue. In this study, we report a novel strategy to "engineer" controlled 3D nanocomposite fibrous matrix of poly(ε-caprolactone) (PCL) and silk fibroin (SF) for an artificial dermis application. Using a custom-designed cold-plate electrospinning and automatic magnet agitation system, up to 6mm of the thickness was achieved resulting from the accumulation of ice crystal layers on the PCL nanofibers surface-modified with the SF particles. The sacrificed ice crystals induced interconnected macro-pores ranging from tens to hundreds μm. The agitation system introduced uniform distribution of the SF protein within/on the nanofibers, preventing the particles from precipitation and agglomeration. NIH 3T3 fibroblasts proliferated in vitro on the PCL and PCL/SF scaffolds for 7days, but there was no statistical difference between the groups. Conversely, In vivo rat model studies revealed that the wound healing rate and collagen deposition increased with the SF content within the nanocomposites. The unique 3D construct with the PCL/SF nanocomposite fibers provided desirable spatial cues, surface topography, and surface chemistry for the native cells to infiltrate into the scaffolds. The wound healing potential of the nanocomposites was comparable to the commercial Matriderm® artificial dermis.
authors: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea. Electronic address: yiyi1124@gmail.com.
authors: Jung Min
authors: JM
authors: Lee
authors: Department of Materials Engineering, University of British Columbia, Vancouver, BC V6T 1Z4, Canada. Electronic address: apsiky@gmail.com.
authors: Taesik
authors: T
authors: Chae
authors: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea. Electronic address: faheem99in@yahoo.com.
authors: Faheem A
authors: FA
authors: Sheikh
authors: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea. Electronic address: anabasjoo@gmail.com.
authors: Hyung Woo
authors: HW
authors: Ju
authors: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea. Electronic address: toribom@gmail.com.
authors: Bo Mi
authors: BM
authors: Moon
authors: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea. Electronic address: hyunjungpark869@gmail.com.
authors: Hyun Jung
authors: HJ
authors: Park
authors: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea. Electronic address: payeri89@gmail.com.
authors: Ye Ri
authors: YR
authors: Park
authors: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea; Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Hallym University, Chuncheon 200-704, South Korea. Electronic address: hlpch@paran.com.
authors: Chan Hum
authors: CH
authors: Park
mesh_terms: D000818:Animals; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D053761:Nanocomposites / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017768:Skin, Artificial; D014945:Wound Healing; D014947:Wounds and Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D005349:Fibroins
keywords: 3D electrospinning; Artificial dermis; Biopolymers; Cell infiltration