title: Dynamic transcriptome profiling of Bean Common Mosaic Virus (BCMV) infection in Common Bean (Phaseolus vulgaris L.).
abstract: Bean common mosaic virus (BCMV) is widespread, with Phaseolus species as the primary host plants. Numerous BCMV strains have been identified on the basis of a panel of bean varieties that distinguish the pathogenicity types with respect to the viral strains. The molecular responses in Phaseolus to BCMV infection have not yet been well characterized.
  
  
  
  We report the transcriptional responses of a widely susceptible variety of common bean (Phaseolus vulgaris L., cultivar 'Stringless green refugee') to two BCMV strains, in a time-course experiment. We also report the genome sequence of a previously unreported BCMV strain. The interaction with the known strain NL1-Iowa causes moderate symptoms and large transcriptional responses, and the newly identified strain (Strain 2 or S2) causes severe symptoms and moderate transcriptional responses. The transcriptional profiles of host plants infected with the two isolates are distinct, and involve numerous differences in splice forms in particular genes, and pathway specific expression patterns.
  
  
  
  We identified differential host transcriptome response after infection of two different strains of Bean common mosaic virus (BCMV) in common bean (Phaseolus vulgaris L.). Virus infection initiated a suite of changes in gene expression level and patterns in the host plants. Pathways related to defense, gene regulation, metabolic processes, photosynthesis were specifically altered after virus infection. Results presented in this study can increase the understanding of host-pathogen interactions and provide resources for further investigations of the biological mechanisms in BCMV infection and defense.
authors: Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA.
authors: Kathleen
authors: K
authors: Martin
authors: ORISE Fellow, USDA-ARS, Corn Insects and Crop Genetics Research Unit, Ames, IA, 50011, USA.
authors: Jugpreet
authors: J
authors: Singh
authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames, 50011, USA.
authors: John H
authors: JH
authors: Hill
authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames, 50011, USA.
authors: Steven A
authors: SA
authors: Whitham
authors: Department of Agronomy, Iowa State University, Ames, IA, 50011, USA. steven.cannon@ars.usda.gov.|USDA-ARS, Corn Insects and Crop Genetics Research Unit, Crop Genome Informatics Laboratory, Iowa State University, Ames, IA, 50011, USA. steven.cannon@ars.usda.gov.
authors: Steven B
authors: SB
authors: Cannon
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D054884:Host-Pathogen Interactions; D058977:Molecular Sequence Annotation; D027805:Phaseolus / Q000235:genetics* / Q000276:immunology / Q000821:virology; D010788:Photosynthesis / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology; D057865:Plant Immunity / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology; D017800:Potyvirus / Q000235:genetics / Q000472:pathogenicity*; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D010940:Plant Proteins
keywords: Bean common mosaic virus; Gene expression; Gene regulation; Host-virus interaction; Phaseolus vulgaris; Regulatory changes