title: Functional engineered human cardiac patches prepared from nature's platform improve heart function after acute myocardial infarction.
abstract: With the advent of induced pluripotent stem cells and directed differentiation techniques, it is now feasible to derive individual-specific cardiac cells for human heart tissue engineering. Here we report the generation of functional engineered human cardiac patches using human induced pluripotent stem cells-derived cardiac cells and decellularized natural heart ECM as scaffolds. The engineered human cardiac patches can be tailored to any desired size and shape and exhibited normal contractile and electrical physiology in vitro. Further, when patching on the infarct area, these patches improved heart function of rats with acute myocardial infarction in vivo. These engineered human cardiac patches can be of great value for normal and disease-specific heart tissue engineering, drug screening, and meet the demands for individual-specific heart tissues for personalized regenerative therapy of myocardial damages in the future.
authors: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors: Qingjie
authors: Q
authors: Wang
authors: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors: Hui
authors: H
authors: Yang
authors: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors: Aobing
authors: A
authors: Bai
authors: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors: Wei
authors: W
authors: Jiang
authors: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors: Xiuya
authors: X
authors: Li
authors: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors: Xinhong
authors: X
authors: Wang
authors: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors: Yishen
authors: Y
authors: Mao
authors: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors: Chao
authors: C
authors: Lu
authors: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors: Ruizhe
authors: R
authors: Qian
authors: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors: Feng
authors: F
authors: Guo
authors: Department of Hematology, Huashan Hospital, Fudan University, Shanghai, 200032, China.
authors: Tianling
authors: T
authors: Ding
authors: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors: Haiyan
authors: H
authors: Chen
authors: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors: Sifeng
authors: S
authors: Chen
authors: Department of Biomedical Engineering, University of Alabama, Birmingham, AL, 35294, USA.
authors: Jianyi
authors: J
authors: Zhang
authors: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. Electronic address: liu.chen@zs-hospital.sh.cn.
authors: Chen
authors: C
authors: Liu
authors: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China; Research Center on Aging and Medicine, Fudan University, Shanghai, 200032, China; State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200032, China. Electronic address: sunning@fudan.edu.cn.
authors: Ning
authors: N
authors: Sun
mesh_terms: D000208:Acute Disease; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002474:Cell-Free System; D005109:Extracellular Matrix / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000637:transplantation*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000637:transplantation*; D008297:Male; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000637:transplantation; D009924:Organ Culture Techniques / Q000295:instrumentation / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds; D016896:Treatment Outcome
keywords: Cardiac patch; Cardiomyocytes; Decellularized heart matrix; Engineering human heart tissue; Induced pluripotent stem cells; Myocardial infarction