title: A TPA-caged precursor of (imino)coumarin for "turn-on" fluorogenic detection of Cu(.).
abstract: We strategize to utilize the precursors of (imino)coumarin fluorophores to deliver novel reactive Cu(+) probes, where tris[(2-pyridyl)-methyl] amine (TPA) works as a reactive receptor towards Cu(+). To verify this strategy, CP1, a representative probe and relevant sensing behaviors towards Cu(+) are presented here. CP1 features good solubility and fast response for monitoring labile copper in aqueous solution and live cells. The sensing mechanism of CP1 is determined by HPLC titration and mass spectrometric analysis. The probe CP1 exhibits a 60-fold fluorescence enhancement and a detection limitation of 10.8 nM upon the detection of Cu(+). CP1 is further applied for imaging labile copper in live cells. This work provides a starting point for future development of Cu(+) probes, based on in situ formation of (imino)coumarin scaffolds, as well as their further investigations of copper signaling and biological events.
authors: Division of Molecular Surface Physics & Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping 58183, Sweden. Electronic address: zhahu@ifm.liu.se.
authors: Zhangjun
authors: Z
authors: Hu
authors: Division of Molecular Surface Physics & Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping 58183, Sweden; State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China.
authors: Jiwen
authors: J
authors: Hu
authors: Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, Department of Chemistry, Anhui University, Hefei 230039, PR China.
authors: Hui
authors: H
authors: Wang
authors: Division of Molecular Surface Physics & Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping 58183, Sweden.
authors: Qiong
authors: Q
authors: Zhang
authors: Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, Department of Chemistry, Anhui University, Hefei 230039, PR China.
authors: Meng
authors: M
authors: Zhao
authors: Division of Molecular Surface Physics & Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping 58183, Sweden.
authors: Caroline
authors: C
authors: Brommesson
authors: Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, Department of Chemistry, Anhui University, Hefei 230039, PR China.
authors: Yupeng
authors: Y
authors: Tian
authors: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China.
authors: Hongwen
authors: H
authors: Gao
authors: Division of Molecular Surface Physics & Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping 58183, Sweden; Faculty of Health Sciences, University of Macau, Taipa, Macau, PR China. Electronic address: xuanjunzhang@umac.mo.
authors: Xuanjun
authors: X
authors: Zhang
authors: Division of Molecular Surface Physics & Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping 58183, Sweden.
authors: Kajsa
authors: K
authors: Uvdal
mesh_terms: D003300:Copper / Q000032:analysis*; D003374:Coumarins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007097:Imines / Q000737:chemistry*; D015394:Molecular Structure; D011725:Pyridines / Q000737:chemistry*
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D007097:Imines; D011725:Pyridines; D003300:Copper; C030123:coumarin
keywords: (Imino)coumarin; Copper ions; Fluorogenic probe; Reaction-based fluorescence