title: Effect of Recombinant Human Prolactin Addition to Biolasol Solution on Biochemical Indicators in Perfundates of Porcine Kidneys.
abstract: BACKGROUND
  The influence of recombinant human prolactin (rh-PRL) added to Biolasol solution (concentration 1 μg/L) on selected markers (pH, osmolarity, Na(I) and K(I) concentration) and enzymatic activity (alanine aminotransferase [ALT], aspartate aminotransferase [AST], and lactate dehydrogenase [LDH]) in perfundates was investigated during flushing and preservation of the isolated porcine kidneys.
  
  
  METHODS
  The pH, osmolarity, concentration of K(I) and Na(I), and enzymatic activity were determined in perfundates collected after the 5th and 30th minutes of perfusion, after 24 hours of organ preservation, and in the 5th and 30th minutes of reperfusion. Kidneys had been flushed and stored in Biolasol (control group) and in Biolasol with rh-PRL (experimental group). Obtained results were compared with Biolasol solution.
  
  
  RESULTS
  In the experimental group, the decrease in pH value in the 5th minute of reperfusion was noted. There was an increase in K(I) concentration, and Na(I) concentration decreased in the 5th and 30th minutes of reperfusion. ALT activity during perfusion and preservation increased, whereas at the 5th and 30th minutes of reperfusion it decreased. AST activity increased during perfusion and preservation and decreased in the 5th and 30th minutes of reperfusion. LDH activity was increased but decreased in the 5th minute of reperfusion.
  
  
  CONCLUSIONS
  Addition of 1 μg/L rh-PRL to Biolasol solution decreases pH and osmolarity values; influences Na(I) and K(I) concentration; increases ALT, AST activity during perfusion and preservation of organs; and decreases ALT, AST activity during reperfusion.
authors: Pharmaceutical Research and Production Plant "Biochefa", Sosnowiec, Poland. Electronic address: info@biochefa.pl.
authors: F
authors: F
authors: Ryszka
authors: Pharmaceutical Research and Production Plant "Biochefa", Sosnowiec, Poland; Department of Applied Pharmacy, Medical University of Silesia, Sosnowiec, Poland.
authors: B
authors: B
authors: Dolińska
authors: Pharmaceutical Research and Production Plant "Biochefa", Sosnowiec, Poland.
authors: K
authors: K
authors: Czyż
authors: Pharmaceutical Research and Production Plant "Biochefa", Sosnowiec, Poland.
authors: M
authors: M
authors: Jelińska
authors: Pharmaceutical Research and Production Plant "Biochefa", Sosnowiec, Poland.
authors: A
authors: A
authors: Strabel
authors: Pharmaceutical Research and Production Plant "Biochefa", Sosnowiec, Poland.
authors: J
authors: J
authors: Bocheńska
mesh_terms: D000818:Animals; D006801:Humans; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism*; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000737:chemistry* / Q000494:pharmacology*; D010477:Perfusion; D011388:Prolactin / Q000494:pharmacology*; D013552:Swine
chemical_list: D019188:Organ Preservation Solutions; D011388:Prolactin