title: Chimeric VLPs with GII.3 P2 domain in a backbone of GII.4 VP1 confers novel HBGA binding ability.
abstract: Noroviruses (NoVs) are a leading cause of non-bacterial acute gastroenteritis worldwide. The prevalence of Genogroup II, genotype 3 (GII.3) NoVs is secondary to the epidemic GII.4 strains which show broad spectrum binding activities against multiple types of histo-blood group antigens (HBGAs). In our previous study it was found that GII.3 NoV VLPs exhibited no binding activity to all synthetic and salivary HBGAs tested. To determine the compatibility of P2 domains between different genotypes and its effect over the binding specificity to HBGAs, we swapped the P2 domain of a GII.4 strain (Sydney 2012-like variant) with that of a GII.3 strain (GII.4-VP1/GII.3-P2). In vitro VLP-HBGA binding and binding blockade assays were used to characterize the binding patterns of GII.4-VP1/GII.3-P2 chimeric capsid protein. Expression of GII.4-VP1/GII.3-P2 chimeric capsid protein using recombinant bacuolovirus expression system led to assembly of virus like particles (VLPs). In vitro VLP-HBGA binding assay using synthetic and salivary HBGAs indicated binding activities to blood type A (trimer), Le(x) and blood type A, B and O salivary HBGAs. In vitro VLP-HBGA binding blockade assay indicated that the binding could be blocked by rabbit hyperimmune serum against GII.3 VLPs, but not hyperimmune sera against GI.2 and GII.4 VLPs. These results indicate that the observed binding activities may be caused by conformational changes of inserted P2 domain and possibly reflect the actual binding profile of GII.3 VLPs. The currently observed absence of binding of GII.3 NoV VLPs to salivary or synthetic HBGAs might be due to absence of other unknown factors.
authors: The Sixth People's Hospital of Zhengzhou, Zhengzhou, PR China. Electronic address: 1246105971@qq.com.
authors: Yuqi
authors: Y
authors: Huo
authors: Wuhan Institute of Biological Products, Wuhan, PR China.
authors: Wenhui
authors: W
authors: Wang
authors: Wuhan Institute of Biological Products, Wuhan, PR China.
authors: Tong
authors: T
authors: Ling
authors: Wuhan Institute of Biological Products, Wuhan, PR China.
authors: Xin
authors: X
authors: Wan
authors: Wuhan Institute of Biological Products, Wuhan, PR China.
authors: Li
authors: L
authors: Ding
authors: Wuhan Institute of Biological Products, Wuhan, PR China. Electronic address: shenshuo1@sinopharm.com.
authors: Shuo
authors: S
authors: Shen
authors: Zhengzhou University, Zhengzhou, PR China.
authors: Jinling
authors: J
authors: Huo
authors: Zhengzhou University, Zhengzhou, PR China.
authors: Shanfeng
authors: S
authors: Zhang
authors: Zhengzhou University, Zhengzhou, PR China.
authors: Mingchen
authors: M
authors: Wang
authors: The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, PR China.
authors: Yumei
authors: Y
authors: Wang
authors: The Sixth People's Hospital of Zhengzhou, Zhengzhou, PR China.
authors: Yubing
authors: Y
authors: Liu
mesh_terms: D001789:Blood Group Antigens / Q000378:metabolism*; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics; D005759:Gastroenteritis / Q000821:virology*; D005838:Genotype; D006801:Humans; D007106:Immune Sera / Q000378:metabolism; D029322:Norovirus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D000072417:Protein Domains; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D014771:Virion / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001789:Blood Group Antigens; D036022:Capsid Proteins; D007106:Immune Sera; D011993:Recombinant Fusion Proteins; D012471:Salivary Proteins and Peptides
keywords: Chimeric capsid protein; Conformation; Histo-blood group antigens; Norovirus