title: The ACE2 G8790A Polymorphism: Involvement in Type 2 Diabetes Mellitus Combined with Cerebral Stroke.
abstract: BACKGROUND
  We aimed to investigate the correlations between ACE2 polymorphisms and type 2 diabetes mellitus (T2DM) combined with cerebral stroke (CS).
  
  
  METHODS
  A total of 346 patients treated or hospitalized in our hospital were enrolled, including 181 cases without cerebrovascular complications (T2DM group) and 165 cases combined with CS (T2DM + CS group); 284 healthy individuals were selected as the control group. PCR-RFLP and ELISA were used to analyze ACE2 G8790A polymorphisms and serum ACE2 levels, respectively.
  
  
  RESULTS
  Significant differences were observed in the genotype/allele frequency of ACE2 G8790A between the T2DM + CS and control groups, and the T2DM and T2DM + CS groups, and in the genotype frequency of ACE2 G8790A between the T2DM and the control groups. The A allele may increase the risk of T2DM combined with CS. The AA genotype may also increase the risk of T2DM combined with CS (OR = 3.733, 95%CI = 2.069-6.738; OR = 3.597, 95%CI = 1.884-6.867). Serum ACE2 levels showed statistically significant differences among the groups. Systolic pressure and diastolic pressure were protective factors of T2DM combined with CS.
  
  
  CONCLUSIONS
  The ACE2 G8790A polymorphism in T2DM patients was correlated with CS, and the A allele might be a risk factor of T2DM combined with CS.
authors: Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors: Yan-Hui
authors: YH
authors: Wu
authors: Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors: Jia-Ying
authors: JY
authors: Li
authors: Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors: Chi
authors: C
authors: Wang
authors: Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors: Li-Mei
authors: LM
authors: Zhang
authors: Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors: Hong
authors: H
authors: Qiao
mesh_terms: D000085962:Angiotensin-Converting Enzyme 2; D001794:Blood Pressure; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D007703:Peptidyl-Dipeptidase A / Q000097:blood* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D020521:Stroke / Q000097:blood / Q000235:genetics*
chemical_list: D007703:Peptidyl-Dipeptidase A; C000705307:ACE2 protein, human; D000085962:Angiotensin-Converting Enzyme 2
keywords: 
  ACE2
  ; allele frequency; cerebral stroke; gene polymorphism; regression analysis; type 2 diabetes mellitus