title: Transcriptional regulation of E-cadherin by small activating RNA: A new double-stranded RNA.
abstract: Recent studies have reported that chemically synthesized small activating RNA (saRNA) targeting the promoter regions of a gene can activate its expression in different cell lines. This technique can be a powerful therapeutic method for diseases caused by complete inactivation or reduced expression of specific genes. E-cadherin is a typical tumor suppressor gene. Loss of E-cadherin mediates the transition from benign lesions to invasive, metastatic cancer. In this study, several 21-nt small double-stranded RNAs (dsRNAs) targeting the promoter regions of human E-cadherin were designed and synthesized and the features of their function were investigated to study the regulatory role of dsRNA on E-cadherin expression. A new saRNA (dsEcad‑661) that can enhance E-cadherin expression by targeting non-coding regulatory regions in gene promoters was identified. Using dsRNA with modified base quantity and cholesterol-conjugated dsRNA, we found the antisense strand may be the guide strand of saRNA in the upregulation of E-cadherin. These findings provide several important pieces of evidence that may improve understanding of the function of saRNA and may promote its development for clinical application.
authors: Department of Urology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, P.R. China.
authors: Zhiming
authors: Z
authors: Wu
authors: Department of Urology, Jinshan Hospital, Fudan University, Shanghai, P.R. China.
authors: Yan
authors: Y
authors: Li
authors: Department of Urology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, P.R. China.
authors: Zhiyong
authors: Z
authors: Li
authors: Department of Urology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, P.R. China.
authors: Zhuowei
authors: Z
authors: Liu
authors: Department of Urology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, P.R. China.
authors: Zike
authors: Z
authors: Qin
authors: Department of Urology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, P.R. China.
authors: Xiangdong
authors: X
authors: Li
authors: Department of Urology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, P.R. China.
authors: Yunlin
authors: Y
authors: Ye
authors: Langone Medical Center School of Medicine, NYU, New York, NY, USA.
authors: Lei
authors: L
authors: Bu
authors: Langone Medical Center School of Medicine, NYU, New York, NY, USA.
authors: Bin
authors: B
authors: Lin
authors: Department of Urology, Jinshan Hospital, Fudan University, Shanghai, P.R. China.
authors: Zhanyu
authors: Z
authors: Wang
authors: Department of Urology, Jinshan Hospital, Fudan University, Shanghai, P.R. China.
authors: Guojin
authors: G
authors: Jia
authors: Department of Urology, Jinshan Hospital, Fudan University, Shanghai, P.R. China.
authors: Gang
authors: G
authors: Chen
mesh_terms: D015703:Antigens, CD; D014408:Biomarkers, Tumor / Q000235:genetics; D015153:Blotting, Western; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008856:Microscopy, Fluorescence; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D012330:RNA, Double-Stranded / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D050436:Regulatory Elements, Transcriptional / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C513384:CDH1 protein, human; D015820:Cadherins; D012330:RNA, Double-Stranded; D012333:RNA, Messenger