title: A comparative evaluation of different ionic liquids for arsenic species separation and determination in wine varietals by liquid chromatography - hydride generation atomic fluorescence spectrometry.
abstract: The application of different ionic liquids (ILs) as modifiers for chromatographic separation and determination of arsenite [As(III)], arsenate [As(V)], dimethylarsonic acid (DMA) and monomethylarsonic acid (MMA) species in wine samples, by reversed-phase high performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry detection (RP-HPLC-HG-AFS) was studied in this work. Several factors influencing the chromatographic separation of the As species, such as pH of the mobile phase, buffer solution concentration, buffer type, IL concentration and length of alkyl groups in ILs were evaluated. The complete separation of As species was achieved using a C18 column in isocratic mode with a mobile phase composed of 0.5% (v/v) 1-octyl-3-methylimidazolium chloride ([C8mim]Cl) and 5% (v/v) methanol at pH 8.5. A multivariate methodology was used to optimize the variables involved in AFS detection of As species after they were separated by HPLC. The ILs showed remarkable performance for the separation of As species, which was obtained within 18min with a resolution higher than 0.83. The limits of detection for As(III), As(V), MMA and DMA were 0.81, 0.89, 0.62 and 1.00μg As L(-1). The proposed method was applied for As speciation analysis in white and red wine samples originated from different grape varieties.
authors: Laboratory of Analytical Chemistry for Research and Development (QUIANID), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Padre J. Contreras 1300, P.C 5500 Mendoza, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors: Alexander
authors: A
authors: Castro Grijalba
authors: Laboratory of Analytical Chemistry for Research and Development (QUIANID), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Padre J. Contreras 1300, P.C 5500 Mendoza, Argentina.
authors: Emiliano F
authors: EF
authors: Fiorentini
authors: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; INQUISAL-CONICET, Departamento de Química Analítica, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina.
authors: Luis D
authors: LD
authors: Martinez
authors: Laboratory of Analytical Chemistry for Research and Development (QUIANID), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Padre J. Contreras 1300, P.C 5500 Mendoza, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: rwuilloud@mendoza-conicet.gob.ar.
authors: Rodolfo G
authors: RG
authors: Wuilloud
mesh_terms: D001151:Arsenic / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D001152:Arsenicals / Q000032:analysis / Q000302:isolation & purification; D018053:Arsenites / Q000032:analysis / Q000302:isolation & purification; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry*; D057230:Limit of Detection; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013054:Spectrophotometry, Atomic / Q000379:methods*; D027843:Vitis / Q000737:chemistry / Q000145:classification; D014920:Wine / Q000032:analysis*
chemical_list: D001152:Arsenicals; D018053:Arsenites; D002021:Buffers; D052578:Ionic Liquids; C054806:arsonic acid; C020300:monomethylarsonic acid; C015001:arsenite; D001151:Arsenic
keywords: Arsenic speciation; Atomic fluorescence spectrometry; Ionic liquids; Liquid chromatography; Wines