title: Piptadenin, a Novel 3,4-Secooleanane Triterpene and Piptadenamide, a New Ceramide from the Stem Bark of Piptadeniastrum africanum (Hook.f.) Brenan.
abstract: Piptadenin (1), a new triterpene along with piptadenamide (10), a new ceramide, have been isolated from the AcOEt-soluble fraction of the MeOH extract of the stem bark of Piptadeniastrum africanum along with nine known compounds, 1-O-[(3β,22β)-3,22-dihydroxy-28-oxoolean-12-en-28-yl]-β-d-glucopyranose (2), 22β-hydroxyoleanic acid (3), oleanic acid (4), lupeol (5), betulinic acid (6), 5α-stigmasta-7,22-dien-3β-ol (7), 5α-stigmasta-7,22-dien-3-one (8), (3β)-stigmast-5-en-3-yl β-d-glucopyranoside (9) and 2,3-dihydroxypropyl hexacosanoate (11). Except for compound 11, all the isolated compounds are reported for the first time from this plant. The structures of the isolated compounds were elucidated by spectroscopic data including 1D and 2D NMR. The pure compounds 1 - 11 were subjected to the pharmacological screening and compounds 2, 5 - 7 and 9 exhibited potent urease inhibitory activity with IC50value of 25.8, 28.9, 30.1, 31.8 and 32.7 μm, respectively, whereas compound 1 showed moderate activity (IC50= 98.7 μm). The potent urease inhibitory activity supplemented the previous literature reports and medicinal uses of this plant.
authors: Department of Chemistry, Higher Teachers Training College, University of Maroua, P.O. Box 55, Maroua, Cameroon.
authors: Amadou
authors: A
authors: Dawé
authors: Department of Biology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
authors: Marius
authors: M
authors: Mbiantcha
authors: Department of Chemistry, Higher Teachers Training College, University of Maroua, P.O. Box 55, Maroua, Cameroon.
authors: Yannick
authors: Y
authors: Fongang
authors: Department of Biology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
authors: William Yosseu
authors: WY
authors: Nana
authors: Department of Chemistry, Higher Teachers Training College, University of Maroua, P.O. Box 55, Maroua, Cameroon.
authors: Fawai
authors: F
authors: Yakai
authors: Department of Biology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
authors: Gilbert
authors: G
authors: Ateufack
authors: International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
authors: Muhammad Ali
authors: MA
authors: Shaiq
authors: PCSIR Laboratories Karachi Complex, Pharmaceutical Research Center, Karachi, 75280, Pakistan.
authors: Iqbal
authors: I
authors: Lubna
authors: PCSIR Laboratories Karachi Complex, Pharmaceutical Research Center, Karachi, 75280, Pakistan.
authors: Mehreen
authors: M
authors: Lateef
authors: Department of Organic Chemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
authors: Bonaventure Tchaleu
authors: BT
authors: Ngadjui
mesh_terms: D002518:Ceramides / Q000737:chemistry / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*; D014510:Urease / Q000037:antagonists & inhibitors
chemical_list: D002518:Ceramides; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D014315:Triterpenes; C000614475:piptadenin; D014510:Urease
keywords: 
  Piptadeniastrum africanum
  ; 3,4-Secooleanane triterpene; Ceramide; Leguminosae; Urease inhibition