title: Epineurium-mimicking chitosan conduits for peripheral nervous tissue engineering.
abstract: In this investigation, we report on a fabrication method of epineurium-mimicking tubular conduits based on electrodeposition from chitosan solution. The pre-enrichment of electrodeposition solution with hyaluronic acid and/or collagen components results in structures which structural, morphological, and physicochemical properties can be controlled. In order to determine the optimal composition of the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants, we perform chemical, physical, and biological studies. Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits. Because, the obtained structures show biocompatibility when contacting with a mouse hippocampal cell line (mHippoE-18), we further plan to test their application potential on an animal model.
authors: Department of Process Thermodynamics, Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213, 90-924 Lodz, Poland. Electronic address: katarzyna.nawrotek@p.lodz.pl.
authors: Katarzyna
authors: K
authors: Nawrotek
authors: Department of Process Thermodynamics, Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213, 90-924 Lodz, Poland. Electronic address: michal.tylman@p.lodz.pl.
authors: Michał
authors: M
authors: Tylman
authors: Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland. Electronic address: rudnicka@biol.uni.lodz.pl.
authors: Karolina
authors: K
authors: Rudnicka
authors: Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland. Electronic address: gatjus@biol.uni.lodz.pl.
authors: Justyna
authors: J
authors: Gatkowska
authors: Department of Neurobiology, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. Electronic address: marek@biol.uni.lodz.pl.
authors: Marek
authors: M
authors: Wieczorek
mesh_terms: D000818:Animals; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D009417:Nerve Tissue / Q000166:cytology / Q000378:metabolism*; D010525:Peripheral Nerves; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D048271:Chitosan
keywords: Biomaterial; Chitosan; Electrodeposition; Hydrogel; Nerve regeneration