title: Fast Targeting and Cancer Cell Uptake of Luminescent Antibody-Nanozeolite Bioconjugates.
abstract: Understanding the targeted cellular uptake of nanomaterials is an essential step to engineer and program functional and effective biomedical devices. In this respect, the targeting and ultrafast uptake of zeolite nanocrystals functionalized with Cetuximab antibodies (Ctxb) by cells overexpressing the epidermal growth factor receptor are described here. Biochemical assays show that the cellular uptake of the bioconjugate in the targeted cancer cells already begins 15 min after incubation, at a rate around tenfold faster than that observed in the negative control cells. These findings further show the role of Ctxb exposed at the surfaces of the zeolite nanocrystals in mediating the targeted and rapid cellular uptake. By using temperature and pharmacological inhibitors as modulators of the internalization pathways, the results univocally suggest a dissipative uptake mechanism of these nanomaterials, which seems to occur using different internalization pathways, according to the targeting properties of these nanocrystals. Owing to the ultrafast uptake process, harmless for the cell viability, these results further pave the way for the design of novel theranostic tools based on nanozeolites.
authors: Namur Research College (NARC) and Department of Chemistry, University of Namur, Rue de Bruxelles 61, Namur, B-5000, Belgium.
authors: Riccardo
authors: R
authors: Marega
authors: Institut de science et d'Ingénierie Supramoléculaire (ISIS), Université de Strasbourg, 8 Rue Gaspard Monge, BP 70028, Strasbourg, F-67000, France.|Karlsruher Institut für Technologie KIT-INT, Karlsruhe, D-76131, Germany.
authors: Eko Adi
authors: EA
authors: Prasetyanto
authors: Cellular Biology Research Unit - NARILIS, University of Namur, Rue de Bruxelles 61, Namur, B-5000, Belgium.
authors: Carine
authors: C
authors: Michiels
authors: Institut de science et d'Ingénierie Supramoléculaire (ISIS), Université de Strasbourg, 8 Rue Gaspard Monge, BP 70028, Strasbourg, F-67000, France. decola@unistra.fr.|Karlsruher Institut für Technologie KIT-INT, Karlsruhe, D-76131, Germany. decola@unistra.fr.
authors: Luisa
authors: L
authors: De Cola
authors: Namur Research College (NARC) and Department of Chemistry, University of Namur, Rue de Bruxelles 61, Namur, B-5000, Belgium. bonifazid@cardiff.ac.uk.|School of Chemistry, Cardiff University, Park Place, Cardiff, CF10 3AT, United Kingdom. bonifazid@cardiff.ac.uk.
authors: Davide
authors: D
authors: Bonifazi
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D001672:Biocompatible Materials / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D000068818:Cetuximab / Q000737:chemistry / Q000494:pharmacology; D004705:Endocytosis; D066246:ErbB Receptors / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D049449:Luminescence; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000473:pathology*; D056951:Photoelectron Spectroscopy; D013997:Time Factors; D017641:Zeolites / Q000378:metabolism*
chemical_list: D000906:Antibodies; D001672:Biocompatible Materials; D017641:Zeolites; D066246:ErbB Receptors; D000068818:Cetuximab
keywords: Cetuximab; cellular uptake; nanomaterials; targeting; zeolites