title: (E)-9-oxooctadec-10-en-12-ynoic acid from Ixora brachiata Roxb. increases glucose uptake in L6 myotubes by activating the PI3K pathway.
abstract: In an attempt to discover new scaffolds for anti-diabetic activity from plants, we screened extracts from Ixora brachiata Roxb. for their effect on glucose uptake in L6 myotubes. The petroleum (PE) extract of the plant showed a significant increase in insulin stimulated glucose uptake by L6 myotubes. The bioactivity guided fractionation of the crude extract yielded a compound (E)-9-oxooctadec-10-en-12-ynoic acid (OEA). The compound induced a dose dependent increase in insulin stimulated glucose uptake in L6 myotubes with an EC50of 22.96μM. OEA also increased the phosphorylation of IRS-1, Akt and AS160 leading to increased GLUT4 translocation to the plasma membrane indicating that it promotes insulin stimulated glucose uptake in L6 myotubes by activating the PI3K pathway.
authors: Screening - Metabolic Disorders Translational Unit, Piramal Enterprises Limited, 1A - Nirlon Complex, Off Western Express Highway, Goregaon (East), Mumbai 400 063, Maharashtra, India.
authors: Chandni S
authors: CS
authors: Thakkar
authors: Discovery Analytical Sciences Department, Piramal Enterprises Limited, 1A - Nirlon Complex, Off Western Express Highway, Goregaon (East), Mumbai 400 063, Maharashtra, India.
authors: Rajendra R
authors: RR
authors: Kshirsagar
authors: Natural Products, Piramal Enterprises Limited, 1A - Nirlon Complex, Off Western Express Highway, Goregaon (East), Mumbai 400 063, Maharashtra, India.
authors: Vinayak R
authors: VR
authors: Naik
authors: Centre for Infectious Diseases & Control, School of BioSciences and Technology, VIT University, Vellore 632014, India.
authors: Asit Ranjan
authors: AR
authors: Ghosh
authors: Centre for Organic and Medicinal Chemistry, VIT University, Vellore 632014, India.
authors: V
authors: V
authors: Vijayakumar
authors: Natural Products, Piramal Enterprises Limited, 1A - Nirlon Complex, Off Western Express Highway, Goregaon (East), Mumbai 400 063, Maharashtra, India.
authors: Arvind
authors: A
authors: Saklani
authors: Screening - Metabolic Disorders Translational Unit, Piramal Enterprises Limited, 1A - Nirlon Complex, Off Western Express Highway, Goregaon (East), Mumbai 400 063, Maharashtra, India. Electronic address: asha.almeida@gmail.com.
authors: Asha A
authors: AA
authors: Kulkarni-Almeida
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D053280:Diynes / Q000302:isolation & purification / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification / Q000494:pharmacology*; D020690:GTPase-Activating Proteins / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D019666:Rubiaceae / Q000737:chemistry*; D015398:Signal Transduction
chemical_list: D053280:Diynes; D005231:Fatty Acids, Unsaturated; D020690:GTPase-Activating Proteins; D005947:Glucose; D051275:Glucose Transporter Type 4; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; D019869:Phosphatidylinositol 3-Kinases; D010936:Plant Extracts; D051057:Proto-Oncogene Proteins c-akt; C000613509:(E)-9-oxooctadec-10-en-12-ynoic acid; C526296:Irs1 protein, rat; C495694:Slc2a4 protein, rat; C533101:TBC1D4 protein, rat
keywords: (E)-9-oxooctadec-10-en-12-ynoic acid; Akt; GLUT4; Ixora brachiata; L6 myotubes