title: Early-life seizures alter synaptic calcium-permeable AMPA receptor function and plasticity.
abstract: Calcium (Ca2+)-mediated4signaling pathways are critical to synaptic plasticity. In adults, the NMDA glutamate receptor (NMDAR) represents a major route for activity-dependent synaptic Ca2+entry. However, during neonatal development, when synaptic plasticity is particularly high, many AMPA glutamate receptors (AMPARs) are also permeable to Ca2+(CP-AMPAR) due to low GluA2 subunit expression, providing an additional route for activity- and glutamate-dependent Ca2+influx and subsequent signaling. Therefore, altered hippocampal Ca2+signaling may represent an age-specific pathogenic mechanism. We thus aimed to assess Ca2+responses 48h after hypoxia-induced neonatal seizures (HS) in postnatal day (P)10 rats, a post-seizure time point at which we previously reported LTP attenuation. We found that Ca2+responses were higher in brain slices from post-HS rats than in controls and that this increase was CP-AMPAR-dependent. To determine whether synaptic CP-AMPAR expression was also altered post-HS, we assessed the expression of GluA2 at hippocampal synapses and the expression of long-term depression (LTD), which has been linked to the presence of synaptic GluA2. Here we report a decrease 48h after HS in synaptic GluA2 expression at synapses and LTD in hippocampal CA1. Given the potentially critical role of AMPAR trafficking in disease progression, we aimed to establish whether post-seizure in vivo AMPAR antagonist treatment prevented the enhanced Ca2+responses, changes in GluA2 synaptic expression, and diminished LTD. We found that NBQX treatment prevents all three of these post-seizure consequences, further supporting a critical role for AMPARs as an age-specific therapeutic target.
authors: Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States; Boston Children's Hospital, Boston, MA 02114, United States; Philadelphia College of Osteopathic Medicine, Philadelphia, PA 19131, United States.
authors: Jocelyn J
authors: JJ
authors: Lippman-Bell
authors: Boston Children's Hospital, Boston, MA 02114, United States.
authors: Chengwen
authors: C
authors: Zhou
authors: Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States; Boston Children's Hospital, Boston, MA 02114, United States.
authors: Hongyu
authors: H
authors: Sun
authors: Boston Children's Hospital, Boston, MA 02114, United States.
authors: Joel S
authors: JS
authors: Feske
authors: Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States; Boston Children's Hospital, Boston, MA 02114, United States. Electronic address: frances.jensen@uphs.upenn.edu.
authors: Frances E
authors: FE
authors: Jensen
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D020013:Calcium Signaling; D017774:Long-Term Potentiation; D008297:Male; D051381:Rats; D020318:Rats, Long-Evans; D018091:Receptors, AMPA / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012640:Seizures / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism* / Q000502:physiology
chemical_list: D018091:Receptors, AMPA
keywords: Epilepsy; Glutamate receptor; Neonate; Synaptic plasticity