title: Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes.
abstract: In recent years, the study of glycans is progressing remarkably by the development of glycan analysis systems using mass spectrometry, glycan profiling systems using lectin microarrays, and glycoprotein analysis by the isotope-coded glycosylation site-specific tagging method. With these methodologies, glycan structures and biological functions are being elucidated. In the study of glycan function as well as disease diagnosis, it is important to examine the localization of glycans in tissues and cells. Histochemical methods using lectin probes can localize glycans in the tissues and cells. This chapter describes a pre-embedding electron microscopic method for glycan localization in which tissue sections and cells are incubated with lectin prior to embedding in resin.
authors: Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, 181-8611, Japan. yakimoto@ks.kyorin-u.ac.jp.
authors: Yoshihiro
authors: Y
authors: Akimoto
authors: Gunma Prefectural College of Health Sciences, Maebashi, Gunma, 371-0052, Japan.
authors: Kuniaki
authors: K
authors: Takata
authors: Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, 181-8611, Japan.
authors: Hayato
authors: H
authors: Kawakami
mesh_terms: D000818:Animals; D004853:Epoxy Resins / Q000737:chemistry; D005404:Fixatives / Q000737:chemistry; D005615:Freezing; D005976:Glutaral / Q000737:chemistry; D017979:Gold Colloid / Q000737:chemistry*; D006624:Hippocampus / Q000378:metabolism / Q000648:ultrastructure; D006735:Horseradish Peroxidase / Q000737:chemistry; D007150:Immunohistochemistry / Q000379:methods*; D037102:Lectins / Q000737:chemistry*; D008264:Macrophages / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D008867:Microtomy; D011134:Polysaccharides / Q000378:metabolism*; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D016610:Tissue Embedding / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods
chemical_list: D004853:Epoxy Resins; D005404:Fixatives; D017979:Gold Colloid; D037102:Lectins; D011134:Polysaccharides; C004875:EPON; D006735:Horseradish Peroxidase; D005976:Glutaral
keywords: Colloidal gold; Electron microscopy; Glycans; Horseradish peroxidase (HRP); Lectin; Pre-embedding method