title: The Correlation of Increased CRP Levels with NFKB1 and TLR2 Polymorphisms in the Case of Morbid Obesity.
abstract: Morbid obesity (MO) is associated with an increase in circulating levels of systemic acute phase proteins such as C-reactive protein (CRP). Toll-like receptor is possible candidate for inflammatory responses which is mainly mediated by NFKB1. The aim of this study was to investigate the relationship between NFKB1 and Toll-like receptor (TLR) 2 polymorphisms and the risk of MO in a Turkish population in the context of CRP serum levels which may contribute to susceptibility to the disease. We analysed the distribution of NFKB1-94 ins/del ATTG rs28362491 and TLR2 Arg753Gln rs5743708 polymorphisms using PCR-RFLP method and CRP serum levels using ELISA method in 213 MO and 200 healthy controls. The frequency of the ins/ins genotype and ins allele of rs28362491 was significantly higher in the patients compared to control group (P: 0.0309; P: 0.0421, respectively). Additionally, the frequency of GG genotype and G allele of rs5743708 was found to be statistically higher in the patient group (P: 0.0421; P < 0.0001, respectively). In addition, serum CRP levels (>20 mg/l) in MO patients with ins/ins genotype were significantly higher than in patients with del/ins genotype (P: 0.0309). Serum CRP levels were also higher in MO patients with GG genotype and G allele (P: 0.0001). According to combined analysis, the wild type of rs28362491 and rs5743708 polymorphisms (ins/ins/GG genotype) was also significantly higher in the patient group versus the control group when compared with the combined ins/ins/GA and del/ins/GA genotype (P < 0.0001). Therefore, our findings suggest that rs28362491 and rs5743708 polymorphisms were significantly associated with MO disease through acting by modulating serum CRP levels.
authors: Department of Medical Biology, Cerrahpaşa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors: T
authors: T
authors: Soydas
authors: Division of Endocrinology and Metabolism, Department of Internal Medicine, Bezmialem Medical Faculty, Bezmialem University, Istanbul, Turkey.
authors: O
authors: O
authors: Karaman
authors: Department of Medical Biology, Cerrahpaşa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors: H
authors: H
authors: Arkan
authors: Department of Medical Biology, Cerrahpaşa Medical Faculty, Istanbul University, Istanbul, Turkey. guven.yenmis@yahoo.com.
authors: G
authors: G
authors: Yenmis
authors: Department of Endocrinology and Metabolism, Umraniye Training and Research Hospital, Umraniye, Istanbul, Turkey.
authors: M M
authors: MM
authors: Ilhan
authors: Department of Medical Biology, Cerrahpaşa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors: K
authors: K
authors: Tombulturk
authors: Division of Endocrinology and Metabolism, Department of Internal Medicine, Bezmialem Medical Faculty, Bezmialem University, Istanbul, Turkey.
authors: E
authors: E
authors: Tasan
authors: Department of Medical Biology, Cerrahpaşa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors: G
authors: G
authors: Kanigur Sultuybek
mesh_terms: D000328:Adult; D000483:Alleles; D002097:C-Reactive Protein / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D052002:NF-kappa B p50 Subunit / Q000235:genetics* / Q000276:immunology; D009767:Obesity, Morbid / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D051195:Toll-Like Receptor 2 / Q000235:genetics* / Q000276:immunology
chemical_list: D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2; D002097:C-Reactive Protein