title: Probiotic Conjugated Linoleic Acid Mediated Apoptosis in Breast Cancer Cells by Downregulation of NFκB.
abstract: Conjugated linoleic acid, a functional lipid, produced from Lactobacillus plantarum (LPCLA), has been demonstrated to possess apoptotic activity. The antiproliferative and apoptotic potential of LPCLA was here evaluated in vitro using the MDAMB231 human breast cancer cell line as a model system. Proliferation of MDA MB231 cells was inhibited with increasing concentrations of LPCLA with altered morphological features like cell detachment, rounding of cells and oligonucleosomal fragmentation of DNA. Flow cytometry confirmed the apoptotic potential of LPCLA by ANNEXIN V/PI double staining. Furthermore, outcome results indicated that the apoptosis was mediated by downregulation of the NF?B pathway which in turn acted through proteasome degradation of I?Bα, inhibition of p65 nuclear translocation, release of cytochromeC from mitochondria and finally overexpression of Bax protein. Thus, conjugated linoleic acid, a natural product derived from probiotics, could therefore be a possible potential chemotherapeutic agent due to its apoptotic activity against estrogen receptor negative breast cancer cells.
authors: Sri Padmavati Mahila Viswa Vidhyalayam (SPMVV), Tirupati, Andhra Pradesh, India. Email : umadevi66@gmail.com.
authors: Rashmi Holur
authors: RH
authors: Kadirareddy
authors: Sujana Ghanta
authors: SG
authors: Vemuri
authors: Uma Maheswari Devi
authors: UM
authors: Palempalli
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects*; D005260:Female; D006801:Humans; D044243:Linoleic Acids, Conjugated / Q000627:therapeutic use*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D019936:Probiotics / Q000627:therapeutic use*; D015398:Signal Transduction / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D017304:Annexin A5; D000970:Antineoplastic Agents; D044243:Linoleic Acids, Conjugated; D016328:NF-kappa B; D051028:bcl-2-Associated X Protein