title: Escherichia coli RadD Protein Functionally Interacts with the Single-stranded DNA-binding Protein.
abstract: The bacterial single-stranded DNA binding protein (SSB) acts as an organizer of DNA repair complexes. The radD gene was recently identified as having an unspecified role in repair of radiation damage and, more specifically, DNA double-strand breaks. Purified RadD protein displays a DNA-independent ATPase activity. However, ATP hydrolytic rates are stimulated by SSB through its C terminus. The RadD and SSB proteins also directly interact in vivo in a yeast two-hybrid assay and in vitro through ammonium sulfate co-precipitation. Therefore, it is likely that the repair function of RadD is mediated through interaction with SSB at the site of damage.
authors: From the Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706 slchen3@wisc.edu.
authors: Stefanie H
authors: SH
authors: Chen
authors: From the Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706.
authors: Rose T
authors: RT
authors: Byrne-Nash
authors: From the Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706.
authors: Michael M
authors: MM
authors: Cox
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C476430:SSB protein, E coli; D000251:Adenosine Triphosphatases; C000602559:YejH protein, E coli
keywords: ATPase; DNA repair; SSB; helicase; protein-DNA interaction; protein-protein interaction; radiation biology