title: Induced nanoparticle aggregation for short nucleic acid quantification by depletion isotachophoresis.
abstract: A rapid (<20min) gel-membrane biochip platform for the detection and quantification of short nucleic acids is presented based on a sandwich assay with probe-functionalized gold nanoparticles and their separation into concentrated bands by depletion-generated gel isotachophoresis. The platform sequentially exploits the enrichment and depletion phenomena of an ion-selective cation-exchange membrane created under an applied electric field. Enrichment is used to concentrate the nanoparticles and targets at a localized position at the gel-membrane interface for rapid hybridization. The depletion generates an isotachophoretic zone without the need for different conductivity buffers, and is used to separate linked nanoparticles from isolated ones in the gel medium and then by field-enhanced aggregation of only the linked particles at the depletion front. The selective field-induced aggregation of the linked nanoparticles during the subsequent depletion step produces two lateral-flow like bands within 1cm for easy visualization and quantification as the aggregates have negligible electrophoretic mobility in the gel and the isolated nanoparticles are isotachophoretically packed against the migrating depletion front. The detection limit for 69-base single-stranded DNA targets is 10 pM (about 10 million copies for our sample volume) with high selectivity against nontargets and a three decade linear range for quantification. The selectivity and signal intensity are maintained in heterogeneous mixtures where the nontargets outnumber the targets 10,000 to 1. The selective field-induced aggregation of DNA-linked nanoparticles at the ion depletion front is attributed to their trailing position at the isotachophoretic front with a large field gradient.
authors: Department of Chemical and Biomolecular Engineering, University of Notre Dame, 182 Fitzpatrick Hall, Notre Dame Avenue, Notre Dame, IN, 46556 United States. Electronic address: smarczak@nd.edu.
authors: Steven
authors: S
authors: Marczak
authors: Department of Chemical and Biomolecular Engineering, University of Notre Dame, 182 Fitzpatrick Hall, Notre Dame Avenue, Notre Dame, IN, 46556 United States. Electronic address: satyajyoti.senapati.1@nd.edu.
authors: Satyajyoti
authors: S
authors: Senapati
authors: Department of Chemical and Biomolecular Engineering, University of Notre Dame, 182 Fitzpatrick Hall, Notre Dame Avenue, Notre Dame, IN, 46556 United States. Electronic address: sloukaz@vscht.cz.
authors: Zdenek
authors: Z
authors: Slouka
authors: Department of Chemical and Biomolecular Engineering, University of Notre Dame, 182 Fitzpatrick Hall, Notre Dame Avenue, Notre Dame, IN, 46556 United States. Electronic address: hchang@nd.edu.
authors: Hsueh-Chia
authors: HC
authors: Chang
mesh_terms: D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry; D057625:Isotachophoresis / Q000379:methods*; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D006046:Gold; D004247:DNA
keywords: Ion-selective membrane; Isotachophoresis; Nanoparticle aggregation; Nucleic acid