title: Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora.
abstract: The powder of root bark of Malva parviflora (Malvaceae) was successively extracted with petroleum ether (b.p. 60-80 °C), chloroform and ethanol. The chloroform extract showed antibacterial activity against Staphylococcus aureus and Escherichia coli, whereas the ethanolic extract showed antibacterial activity against only S. aureus. The chloroform extract, after column chromatographic separation on silica gel using petroleum ether:chloroform (3:1) as eluent, furnished 98 mg of white crystalline compound. The yield of the compound is 0.316 % (w/w). The compound has a melting point of 134-136 °C and the Rf value 0.56 in benzene:chloroform:acetone (1:15:1) on silica gel TLC. The compound was characterized as β-sitosterol by physical properties, chemical test, spectral analysis (FTIR, NMR and MS) and comparing the data obtained from the literature.
authors: Chemistry Department, Aksum University, Aksum, Ethiopia.
authors: Mesfin Medihin
authors: MM
authors: Ododo
authors: Chemistry Department, Dilla University, P.O. Box 419, Dilla, Ethiopia.
authors: Manash Kumar
authors: MK
authors: Choudhury
authors: Chemistry Department, Dilla University, P.O. Box 419, Dilla, Ethiopia.
authors: Ahmed Hussen
authors: AH
authors: Dekebo
keywords: Antibacterial activity; Chloroform extract; Ethanolic extract; Malva parviflora; β-Sitosterol