title: Long non-coding RNA MEG3 inhibits microRNA-125a-5p expression and induces immune imbalance of Treg/Th17 in immune thrombocytopenic purpura.
abstract: BACKGROUND
  The imbalance of Treg/Th17 cells is an important pathogenic factor for immune thrombocytopenic purpura (ITP). We previously reported miR-125a-5p targeted CXCL13 and participated in the process of ITP. In the present study, the role of miR-125a-5p in regulating Treg/Th17 ratio and its potential molecular mechanism were investigated.
  
  
  METHODS
  A total of 30 adults with ITP and 30 healthy subjects were included. MEG3 expression in peripheral blood derived CD4+ T cells from ITP patients and healthy subjects were detected by real-time PCR. In vitro experiments, the effects of inhibiting or overexpressing MEG3 on the expression of miR-125a-5p, Foxp3 and ROTγt in CD4+ T cells were investigated.
  
  
  RESULTS
  MEG3 expression was increased in CD4+ T cells of patients with ITP. Dexamethasone decreased MEG3 expression level of CD4+ T cells in vitro. MEG3 directly interacted with miR-125a-5p and MEG3 overexpression inhibited miR-125a-5p expression in CD4+ T cells exposed to dexamethasone. MEG3 down-regulation or miR-125a-5p overexpression promoted Foxp3 expression and inhibited RORγt expression.
  
  
  CONCLUSIONS
  MEG3 interacted with miR-125a-5p and inhibited its expression, and MEG3/miR-125a-5p contributed to induce immune imbalance of Treg/Th17 in ITP.
authors: Department of Hematology, Children's Hospital of Soochow University, Soochow 215003, Jiangsu, China. Electronic address: JianQL023@163.com.
authors: Jian-Qin
authors: JQ
authors: Li
authors: Department of Hematology, Children's Hospital of Soochow University, Soochow 215003, Jiangsu, China.
authors: Shao-Yan
authors: SY
authors: Hu
authors: Jiangsu Institute of Hematology, The First Affiiated Hospital of Soochow University, Soochow 215016, Jiangsu, China.
authors: Zhao-Yue
authors: ZY
authors: Wang
authors: Department of Hematology, Children's Hospital of Soochow University, Soochow 215003, Jiangsu, China.
authors: Jing
authors: J
authors: Lin
authors: Jiangsu Institute of Hematology, The First Affiiated Hospital of Soochow University, Soochow 215016, Jiangsu, China.
authors: Su
authors: S
authors: Jian
authors: Department of Hematology, Children's Hospital of Soochow University, Soochow 215003, Jiangsu, China.
authors: Yong-Chao
authors: YC
authors: Dong
authors: Department of Hematology, Children's Hospital of Soochow University, Soochow 215003, Jiangsu, China.
authors: Xiao-Fang
authors: XF
authors: Wu
authors: Jiangsu Institute of Hematology, The First Affiiated Hospital of Soochow University, Soochow 215016, Jiangsu, China.
authors: Dai-Lan
authors: Jiangsu Institute of Hematology, The First Affiiated Hospital of Soochow University, Soochow 215016, Jiangsu, China.
authors: Li-Juan
authors: LJ
authors: Cao
mesh_terms: D000328:Adult; D001483:Base Sequence; D054382:Chemokine CXCL13 / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000378:metabolism; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics* / Q000276:immunology*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*; D058504:Th17 Cells / Q000187:drug effects / Q000276:immunology*
chemical_list: D054382:Chemokine CXCL13; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C498907:MEG3 non-coding RNA, human; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D062085:RNA, Long Noncoding; C539900:RORC protein, human; D003907:Dexamethasone
keywords: Chemokine; Immune thrombocytopenia; Lymphocytes; miR-125-5p