title: Susceptibility and potential biochemical mechanism of Oedaleus asiaticus to beta-cypermethrin and deltamethrin in the Inner Mongolia, China.
abstract: Oedaleus asiaticus is a highly destructive grass pest in Inner Mongolia, China, and likely developed resistance to pyrethroid insecticides due to their frequent application for control of this locust. In this study, the susceptibility of five field populations of O. asiaticus to two pyrethroid insecticides was investigated. The Wulate Middle Banner (WB) population was the least susceptible, whereas the Ewenki Banner (EB) population appeared to be the most sensitive. The WB population was 3.16 and 5.15-fold less sensitive to beta-cypermethrin and deltamethrin than EB population, respectively. Further, the enzyme activities and mRNA expression levels of carboxylesterase (CarE) and glutathione-S-transferase (GST) were determined and we found that their activities in the WB population were 5.15 and 2.8-fold higher than those in the EB population, respectively. Quantitative real-time PCR (qRT-PCR) analysis demonstrated that the mRNA expression levels of CarE and GST genes were positively correlated with the LD50 in the WB, Siziwang Banner (SB) and EB populations. Our findings suggest that differences in susceptibility to pyrethroids in O. asiaticus might be attributed to the elevated activities and mRNA expression levels of CarE and GST genes.
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China; Inner Mongolia Prataculture Research Center, Hohhot, Inner Mongolia 010031, China.
authors: Wei
authors: W
authors: Dong
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors: Xubo
authors: X
authors: Zhang
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors: Xueyao
authors: X
authors: Zhang
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors: Haihua
authors: H
authors: Wu
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors: Min
authors: M
authors: Zhang
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors: Enbo
authors: E
authors: Ma
authors: Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China. Electronic address: zjz@sxu.edu.cn.
authors: Jianzhen
authors: J
authors: Zhang
mesh_terms: D000818:Animals; D043182:Carboxylesterase / Q000378:metabolism; D002681:China; D005982:Glutathione Transferase / Q000378:metabolism; D006110:Grasshoppers / Q000187:drug effects*; D007305:Insecticide Resistance; D009570:Nitriles / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*
chemical_list: D009570:Nitriles; D011722:Pyrethrins; C017160:cypermethrin; C017180:decamethrin; D005982:Glutathione Transferase; D043182:Carboxylesterase
keywords: Enzyme activity; Oedaleus asiaticus; Pyrethroids; Susceptibility; mRNA expression