title: Deletion of pV affects integrity of capsid causing defect in the infectivity of bovine adenovirus-3.
abstract: Members of the genus Mastadenovirus including bovine adenovirus 3 (BAdV-3) encode a genus-specific unique protein named pV. The pV encoded by BAdV-3 is a protein of 423 aa showing 40.9 % identity to pV of human adenovirus 2. Here, we report the construction and analysis of recombinant BAdV-3 (BAV.dV) containing deletion of pV. The BAV.dV could only be isolated in CRL.pV cells expressing pV, suggesting that pV appears essential for the infection of BAdV-3. Analysis of BAV.dV suggested that despite affecting some late gene expression in virus-infected cells, there was no significant difference in the incorporation of viral proteins in the mature virions. Moreover, analysis of mature virions revealed degraded capsids leading to change in morphology and infectivity of BAV.dV. Furthermore, analysis of the genome sequence of different clones of BAV.dV passaged in different cell lines revealed no mutations in core proteins pVII and pX\Mu suggesting that the replication defect may not be rescued. Our results suggest that pV is required for proper viral assembly of BAdV-3 as lack of pV produces aberrant capsids. Moreover, altered capsids lead to the production of non-infectious BAV.dV virions.
authors: VIDO-InterVac, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.|Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.
authors: Xin
authors: X
authors: Zhao
authors: Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.|Vaccinology & Immunotherapeutics Program, School of Public Health, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.|VIDO-InterVac, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.
authors: Suresh K
authors: SK
authors: Tikoo
mesh_terms: D000257:Adenoviridae Infections / Q000662:veterinary* / Q000821:virology; D000818:Animals; D002213:Capsid / Q000378:metabolism*; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D002460:Cell Line; D017353:Gene Deletion; D017921:Mastadenovirus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D051381:Rats; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014774:Virulence; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D014764:Viral Proteins