title: MicroRNA-215 impairs adipocyte differentiation and co-represses FNDC3B and CTNNBIP1.
abstract: MicroRNAs (miRNAs) are small ∼22 nucleotide regulatory RNAs that regulate the stability and translation of cognate mRNAs. MiRNAs participate in the regulation of adipogenesis, and identification of the full repertoire of miRNAs expressed in adipose tisse is likely to improve our understanding of adipose tissue growth and development significantly. In the present study, miR-215-5p was found to inhibit adipocyte differentiation of 3T3-L1 cells. Moreover, fibronectin type III domain containing 3B (FNDC3B) and catenin, beta interacting protein 1 (CTNNBIP1) were found to be direct targets of miR-215-5p. Further studies in mouse 3T3-L1 cell-line suggests that miR-215-5p is a negative regulator of adipocyte differentiation through post-transcriptional regulation of FNDC3B and CTNNBIP1 during early adipogenesis.
authors: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Hebei Key Laboratory of Veterinary Preventive Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, People's Republic of China.
authors: Yongdong
authors: Y
authors: Peng
authors: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors: Huanan
authors: H
authors: Li
authors: Hebei Key Laboratory of Veterinary Preventive Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, People's Republic of China.
authors: Xianglong
authors: X
authors: Li
authors: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors: Shulong
authors: S
authors: Yu
authors: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors: Hong
authors: H
authors: Xiang
authors: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: pengjianhzau@163.com.
authors: Jian
authors: J
authors: Peng
authors: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: jiangsiwen@mail.hzau.edu.cn.
authors: Siwen
authors: S
authors: Jiang
mesh_terms: D041721:3T3-L1 Cells; D048868:Adaptor Proteins, Signal Transducing; D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics*; D017124:Conserved Sequence; D015536:Down-Regulation / Q000235:genetics; D005353:Fibronectins / Q000235:genetics*; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; C498785:Ctnnbip1 protein, mouse; C538837:Fndc3b protein, mouse; D005353:Fibronectins; C577160:MIRN215 microRNA, mouse; D035683:MicroRNAs; D012097:Repressor Proteins
keywords: Adipocyte differentiation; Adipogenesis; Obesity; Regulate; miR-215