title: Assessment of the repeatability and border-plate effects of the B158/B60 enzyme-linked-immunosorbent assay for the detection of circulating antigens (Ag-ELISA) of Taenia saginata.
abstract: The monoclonal antibody-based circulating antigen detecting ELISA (B158/B60 Ag-ELISA) has been used elaborately in several studies for the diagnosis of human, bovine and porcine cysticercosis. Interpretation of test results requires a good knowledge of the test characteristics, including the repeatability and the effect of the borders of the ELISA plates. Repeatability was tested for 4 antigen-negative and 5 antigen-positive reference bovine serum samples by calculating the Percentage Coefficient of Variation (%CV) within and between plates, within and between runs, overall, for two batches of monoclonal antibodies and by 2 laboratory technicians. All CV values obtained were below 20% (except one: 24.45%), which indicates a good repeatability and a negligible technician error. The value of 24.45% for indicating the variability between batches of monoclonal antibodies for one positive sample is still acceptable for repeatability measures. Border effects were determined by calculating the %CV values between the inner and outer wells of one plate for 2 positive serum samples. Variability is a little more present in the outer wells but this effect is very small and no significant border effect was found.
authors: Department of Biomedical Sciences, Institute of Tropical Medicine, 155 Nationalestraat, B-2000 Antwerp, Belgium; Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, 133 Salisburylaan, B-9820 Merelbeke, Belgium. Electronic address: fjansen@itg.be.
authors: Famke
authors: F
authors: Jansen
authors: Department of Biomedical Sciences, Institute of Tropical Medicine, 155 Nationalestraat, B-2000 Antwerp, Belgium; Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, 133 Salisburylaan, B-9820 Merelbeke, Belgium. Electronic address: pdorny@itg.be.
authors: Pierre
authors: P
authors: Dorny
authors: Department of Biomedical Sciences, Institute of Tropical Medicine, 155 Nationalestraat, B-2000 Antwerp, Belgium. Electronic address: dberkvens@itg.be.
authors: Dirk
authors: D
authors: Berkvens
authors: Department of Biomedical Sciences, Institute of Tropical Medicine, 155 Nationalestraat, B-2000 Antwerp, Belgium. Electronic address: avanhul@itg.be.
authors: Anke
authors: A
authors: Van Hul
authors: Department of Biomedical Sciences, Institute of Tropical Medicine, 155 Nationalestraat, B-2000 Antwerp, Belgium.
authors: Nick
authors: N
authors: Van den Broeck
authors: Department of Biomedical Sciences, Institute of Tropical Medicine, 155 Nationalestraat, B-2000 Antwerp, Belgium.
authors: Caroline
authors: C
authors: Makay
authors: Department of Biomedical Sciences, Institute of Tropical Medicine, 155 Nationalestraat, B-2000 Antwerp, Belgium.
authors: Nicolas
authors: N
authors: Praet
authors: Department of Biomedical Sciences, Institute of Tropical Medicine, 155 Nationalestraat, B-2000 Antwerp, Belgium. Electronic address: sgabriel@itg.be.
authors: Sarah
authors: S
authors: Gabriël
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000097:blood* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041202:Taenia saginata / Q000378:metabolism*; D013622:Taeniasis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D000947:Antigens, Helminth
keywords: Ag-ELISA; Border-plate effect; Repeatability; Taenia saginata