title: Effect of biological factors on successful measurements with skeletal-muscle (1)H-MRS.
abstract: BACKGROUND
  Our purpose in this study was to clarify whether differences in subject group attributes could affect data acquisition in proton magnetic resonance spectroscopy ((1)H-MRS).
  
  
  METHODS
  Subjects without diabetes mellitus (DM) were divided into two groups (group A, in their 20s; group B, 30-60 years old). Subjects with DM formed group C (30-60 years old). The numbers of subjects were 19, 27, and 22 for group A, B, and C respectively. For all subjects, (1)H-MRS measurements were taken of the soleus muscle (SOL) and the anterior tibial muscle (AT). We defined the success of the measurements by the detection of intramyocellular lipids. Moreover, we also measured the full width at half maximum of the water peaks for all subjects.
  
  
  RESULTS
  The success rate was significantly higher for the AT (100%) than for the SOL (81.6%) (P<0.01). For the SOL, the success rate was 100% in group A, 85.2% in group B, and 77.3% in group C. There was a significant difference (P<0.05) between groups A and B, as well as between groups A and C. In all subjects, there was a significant difference (P<0.01) in the full width at half maximum (Hz) of the water peak between the AT and SOL measurements.
  
  
  CONCLUSIONS
  We conclude that differences in the age and DM history of subjects could affect the probability of successful (1)H-MRS data acquisition.
authors: Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors: Tomonori
authors: T
authors: Isobe
authors: Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors: Yoshikazu
authors: Y
authors: Okamoto
authors: Department of Radiology, University of Tsukuba Hospital, Ibaraki, Japan.
authors: Yuji
authors: Y
authors: Hirano
authors: Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
authors: Hiroki
authors: H
authors: Ando
authors: Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors: Kenta
authors: K
authors: Takada
authors: Department of Medical Radiological Technology, Faculty of Health Sciences, Kyorin University, Tokyo, Japan.
authors: Eisuke
authors: E
authors: Sato
authors: Department of Radiology, Tsukuba Medical Center Hospital, Ibaraki, Japan.
authors: Kazuya
authors: K
authors: Shinoda
authors: Department of Medical Radiological Technology, Faculty of Health Sciences, Kyorin University, Tokyo, Japan.
authors: Kiichi
authors: K
authors: Tadano
authors: Department of Radiology, University of Tsukuba Hospital, Ibaraki, Japan.
authors: Hideyuki
authors: H
authors: Takei
authors: Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors: Satoshi
authors: S
authors: Kamizawa
authors: Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
authors: Yutaro
authors: Y
authors: Mori
authors: Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors: Hiroaki
authors: H
authors: Suzuki
keywords: EMCL; FWHM; IMCL; MRS; biological factor; skeletal muscle