title: Methods for Visualization of Neuronal Cilia.
abstract: Neuroscientists have been captivated by cilia ever since these slender, microtubule-based projections on the cell body were found to play critical roles in neuronal specification, maintenance, and function. In mammals, the most common cilia marker, acetylated α-tubulin, is extremely difficult to detect in neuronal cilia. Here, we describe methods to detect neuronal cilia in culture, in fixed sections, and in vivo, taking advantage of transgenic mice carrying fluorescently tagged cilia proteins.
authors: Department of Human Genetics, Emory University School of Medicine, Whitehead Biomedical Research Building, Room 365, 615 Michael St., Suite 301, Atlanta, GA, 30322, USA. tamara.caspary@emory.edu.
authors: Tamara
authors: T
authors: Caspary
authors: Institute of Cell Biology and Neurobiology, Italian National Research Council (CNR), I-00015 Monterotondo Scalo, Rome, Italy.
authors: Daniela
authors: D
authors: Marazziti
authors: Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202, USA.
authors: Nicolas F
authors: NF
authors: Berbari
mesh_terms: D000818:Animals; D002923:Cilia / Q000378:metabolism*; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D009474:Neurons / Q000378:metabolism*; D013194:Staining and Labeling
keywords: ACIII; Acetylated α-tubulin; Arl13b; Cilia-GFP