title: Evaluation of artificial selection in Standard Poodles using whole-genome sequencing.
abstract: Identifying regions of artificial selection within dog breeds may provide insights into genetic variation that underlies breed-specific traits or diseases-particularly if these traits or disease predispositions are fixed within a breed. In this study, we searched for runs of homozygosity (ROH) and calculated the distatistic (which is based upon FST) to identify regions of artificial selection in Standard Poodles using high-coverage, whole-genome sequencing data of 15 Standard Poodles and 49 dogs across seven other breeds. We identified consensus ROH regions ≥1 Mb in length and common to at least ten Standard Poodles covering 0.6 % of the genome, and diregions that most distinguish Standard Poodles from other breeds covering 3.7 % of the genome. Within these regions, we identified enriched gene pathways related to olfaction, digestion, and taste, as well as pathways related to adrenal hormone biosynthesis, T cell function, and protein ubiquitination that could contribute to the pathogenesis of some Poodle-prevalent autoimmune diseases. We also validated variants related to hair coat and skull morphology that have previously been identified as being under selective pressure in Poodles, and flagged additional polymorphisms in genes such as ITGA2B, CBX4, and TNXB that may represent strong candidates for other common Poodle disorders.
authors: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, USA. steven_friedenberg@ncsu.edu.|Comparative Medicine Institute, North Carolina State University, Raleigh, NC, 27607, USA. steven_friedenberg@ncsu.edu.
authors: Steven G
authors: SG
authors: Friedenberg
authors: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, USA.|Comparative Medicine Institute, North Carolina State University, Raleigh, NC, 27607, USA.
authors: Kathryn M
authors: KM
authors: Meurs
authors: Department of Biological Sciences, College of Sciences, North Carolina State University, 3510 Thomas Hall, Raleigh, NC, 27695, USA.|Comparative Medicine Institute, North Carolina State University, Raleigh, NC, 27607, USA.
authors: Trudy F C
authors: TF
authors: Mackay
mesh_terms: D000818:Animals; D001947:Breeding; D004285:Dogs; D016678:Genome / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D006720:Homozygote; D039421:Integrin alpha2 / Q000235:genetics; D010641:Phenotype; D063146:Polycomb-Group Proteins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012641:Selection, Genetic; D019063:Tenascin / Q000235:genetics
chemical_list: D039421:Integrin alpha2; D063146:Polycomb-Group Proteins; D019063:Tenascin; C081865:tenascin X