title: Sulfated polysaccharide fraction from marine algae Solieria filiformis: Structural characterization, gastroprotective and antioxidant effects.
abstract: A sulfated polysaccharide (SFP) fraction from the marine alga Solieria filiformis was extracted and submitted to microanalysis, molar mass estimation and spectroscopic analysis. We evaluated its gastroprotective potential in vivo in an ethanol-induced gastric damage model and its in vitro antioxidant properties (DPPH, chelating ferrous ability and total antioxidant capacity). Its chemical composition revealed to be essentially an iota-carrageenan with a molar mass of 210.9kDa and high degree of substitution for sulfate groups (1.08). In vivo, SFP significantly (P<0.05) reduced, in a dose dependent manner, the ethanol-induced gastric damage. SFP prevents glutathione consume and increase of malondialdehyde and hemoglobin levels. SFP presented an IC50 of 1.77mg/mL in scavenging DPPH. The chelating ferrous ability was 38.98%, and the total antioxidant capacity was 2.01mg/mL. Thus, SFP prevents the development of ethanol-induced gastric damage by reducing oxidative stress in vivo and possesses relevant antioxidant activity in vitro.
authors: Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of ceará, Fortaleza, 60455-760, CE, Brazil.
authors: Willer M
authors: WM
authors: Sousa
authors: LEFFAG - Laboratory of Physiopharmacology Study of Gastrointestinal Tract, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors: Renan O
authors: RO
authors: Silva
authors: Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of ceará, Fortaleza, 60455-760, CE, Brazil.
authors: Francisco F
authors: FF
authors: Bezerra
authors: LEFFAG - Laboratory of Physiopharmacology Study of Gastrointestinal Tract, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors: Rudy D
authors: RD
authors: Bingana
authors: Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of ceará, Fortaleza, 60455-760, CE, Brazil; Federal Institute of Education, Science and Technology of Ceará, Juazeiro do Norte, CE, Brazil.
authors: Francisco Clark N
authors: FCN
authors: Barros
authors: Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of ceará, Fortaleza, 60455-760, CE, Brazil.
authors: Luís E C
authors: LEC
authors: Costa
authors: Laboratory of Polymers, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, 60455-760, CE, Brazil.
authors: Venicios G
authors: VG
authors: Sombra
authors: LEFFAG - Laboratory of Physiopharmacology Study of Gastrointestinal Tract, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil; Department of Morphology, Medical School, Federal University of Ceara, Rua Delmiro de Farias s/n, Rodolfo Teofilo, Fortaleza, CE CEP 60416-030, Brazil.
authors: Pedro M G
authors: PMG
authors: Soares
authors: Laboratory of Polymers, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, 60455-760, CE, Brazil.
authors: Judith P A
authors: JPA
authors: Feitosa
authors: Laboratory of Polymers, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, 60455-760, CE, Brazil.
authors: Regina C M
authors: RCM
authors: de Paula
authors: LEFFAG - Laboratory of Physiopharmacology Study of Gastrointestinal Tract, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors: Marcellus H L P
authors: MHLP
authors: Souza
authors: LAFFEX - Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Parnaíba, 64202-020, PI, Brazil. Electronic address: andreluiz@ufpi.edu.br.
authors: André Luiz R
authors: ALR
authors: Barbosa
authors: Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of ceará, Fortaleza, 60455-760, CE, Brazil.
authors: Ana Lúcia P
authors: ALP
authors: Freitas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000431:Ethanol / Q000633:toxicity; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000461:Rhodophyta / Q000737:chemistry*; D013272:Stomach Diseases / Q000139:chemically induced / Q000517:prevention & control*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D000431:Ethanol
keywords: Antioxidant; Ethanol; Polysaccharides; Solieria filiformis