title: Rotavirus Infections in Children Vaccinated Against Rotavirus in Pune, Western India.
abstract: OBJECTIVE
  To characterize rotavirus infections detected in rotavirus vaccinated children hospitalized for acute gastroenteritis.
  
  
  METHODS
  Observational, hospital-based study.
  
  
  METHODS
  Three hospitals in Pune, Western India.
  
  
  METHODS
  Children aged <5 years hospitalized for acute gastroenteritis during 2013-14.
  
  
  METHODS
  Rotavirus capture ELISA was performed on all stool samples that were collected from patients following informed consent from parents. VP7 and VP4 genes of rotavirus strains were genotyped by multiplex RT-PCR. Stool samples from vaccinated children were tested for other enteric viruses.
  
  
  RESULTS
  Among the 529 children, 53 were vaccinated with at least one dose of the rotavirus vaccine. There was no difference in the mean (SD) (months) age of vaccinated [14.8 (10.6)] and unvaccinated [14.4 (10.5)] children. Rotavirus positivity was significantly higher (47%) in unvaccinated than in vaccinated (28.3%) children (P=0.01). Mean Vesikari score and severe cases were significantly more in rotavirus positive than in negative children within unvaccinated group (P<0.001), while these did not differ within the vaccinated group. Rotavirus strain G1P[8] was identified as the most prevalent strain in both, vaccinated (60%) and unvaccinated (72.8%) groups. No association was found between mean Vesikari score and viral coinfections.
  
  
  CONCLUSIONS
  This study suggests decline in rotavirus positivity in rotavirus-vaccinated children hospitalized for acute gastroenteritis and high prevalence of G1P[8] and non-rotaviral co-infections in Pune, Western India.
authors: Enteric Viruses Group, National Institute of Virology, Pune; *Bharati Vidyapeeth Medical College and Hospital, Pune; Sant Dnyaneshwar Medical Foundation and Research Centres Shaishav Clinic, Pune; King Edward Memorial Hospital, Pune; and National Institute of Epidemiology, Chennai; India. Correspondence to: Dr Shobha D Chitambar, Enteric Viruses Group, National Institute of Virology, 20A, Ambedkar Road, PO Box No 11, Pune 411 001, India. drshobha.niv@gmail.com.
authors: Preeti
authors: P
authors: Jain
authors: Gopalkrishna
authors: G
authors: Varanasi
authors: Rohan
authors: R
authors: Ghuge
authors: Vijay
authors: V
authors: Kalrao
authors: Ram
authors: R
authors: Dhongade
authors: Ashish
authors: A
authors: Bavdekar
authors: Sanjay
authors: S
authors: Mehendale
authors: Shobha
authors: S
authors: Chitambar
mesh_terms: D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D012401:Rotavirus / Q000235:genetics; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000517:prevention & control / Q000821:virology; D022243:Rotavirus Vaccines / Q000627:therapeutic use*
chemical_list: D022243:Rotavirus Vaccines