title: Electrical field assisted matrix solid phase dispersion as a powerful tool to improve the extraction efficiency and clean-up of fluoroquinolones in bovine milk.
abstract: This work presents a new method by electrical matrix solid phase dispersion for the extraction and clean-up of marbofloxacin, ofloxacin, norfloxacin, ciprofloxacin, enrofloxacin, difloxacin and sarafloxacin in bovine milk. Composition and pH of the eluent, applied electrical potential and polarity were optimized by experimental designs. The combination of the chromatographic and electrophoretic mechanisms allowed the extraction and clean-up in one step with low organic solvent consumption, high extraction throughput and elution automation. Linearity, precision, trueness and limit of quantification were evaluated and provided values in accordance with other methods recently developed for the analysis of fluoroquinolones in milk. This technique proved to be promising for the extraction and clean-up of ionizable analytes in different milk matrices.
authors: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Brazil.
authors: Mariana Cristina
authors: MC
authors: da Silva
authors: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Brazil.
authors: Ricardo Mathias
authors: RM
authors: Orlando
authors: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Brazil. Electronic address: adriana@qui.ufmg.br.
authors: Adriana Ferreira
authors: AF
authors: Faria
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D055590:Electromagnetic Phenomena; D024841:Fluoroquinolones / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones
keywords: Bovine milk; Clean-up; Electrical field assisted matrix solid phase dispersion; Extraction; Fluoroquinolones