title: Kdm5/Lid Regulates Chromosome Architecture in Meiotic Prophase I Independently of Its Histone Demethylase Activity.
abstract: During prophase of the first meiotic division (prophase I), chromatin dynamically reorganises to recombine and prepare for chromosome segregation. Histone modifying enzymes are major regulators of chromatin structure, but our knowledge of their roles in prophase I is still limited. Here we report on crucial roles of Kdm5/Lid, one of two histone demethylases in Drosophila that remove one of the trimethyl groups at Lys4 of Histone 3 (H3K4me3). In the absence of Kdm5/Lid, the synaptonemal complex was only partially formed and failed to be maintained along chromosome arms, while localisation of its components at centromeres was unaffected. Kdm5/Lid was also required for karyosome formation and homologous centromere pairing in prophase I. Although loss of Kdm5/Lid dramatically increased the level of H3K4me3 in oocytes, catalytically inactive Kdm5/Lid can rescue the above cytological defects. Therefore Kdm5/Lid controls chromatin architecture in meiotic prophase I oocytes independently of its demethylase activity.
authors: Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom.
authors: Liudmila
authors: L
authors: Zhaunova
authors: Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom.
authors: Hiroyuki
authors: H
authors: Ohkura
authors: Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom.
authors: Manuel
authors: M
authors: Breuer
mesh_terms: D000818:Animals; D002503:Centromere / Q000235:genetics; D020090:Chromosome Segregation / Q000235:genetics; D002875:Chromosomes / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics*; D056466:Histone Demethylases / Q000096:biosynthesis* / Q000235:genetics; D008540:Meiosis / Q000235:genetics*; D049469:Meiotic Prophase I / Q000235:genetics; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism; D013573:Synaptonemal Complex / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; D056466:Histone Demethylases; C518655:Lid protein, Drosophila