title: PCR detection of Burkholderia multivorans in water and soil samples.
abstract: Although semi-selective growth media have been developed for the isolation of Burkholderia cepacia complex bacteria from the environment, thus far Burkholderia multivorans has rarely been isolated from such samples. Because environmental B. multivorans isolates mainly originate from water samples, we hypothesized that water rather than soil is its most likely environmental niche. The aim of the present study was to assess the occurrence of B. multivorans in water samples from Flanders (Belgium) using a fast, culture-independent PCR assay.
  
  
  
  A nested PCR approach was used to achieve high sensitivity, and specificity was confirmed by sequencing the resulting amplicons. B. multivorans was detected in 11 % of the water samples (n = 112) and 92 % of the soil samples (n = 25) tested. The percentage of false positives was higher for water samples compared to soil samples, showing that the presently available B. multivorans recA primers lack specificity when applied to the analysis of water samples.
  
  
  
  The results of the present study demonstrate that B. multivorans DNA is commonly present in soil samples and to a lesser extent in water samples in Flanders (Belgium).
authors: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium.
authors: Charlotte
authors: C
authors: Peeters
authors: Department of Sociology, Ghent University, Ghent, Belgium.|Department of Public Administration and Sociology, Erasmus University Rotterdam, Rotterdam, The Netherlands.
authors: Stijn
authors: S
authors: Daenekindt
authors: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium. Peter.Vandamme@UGent.be.
authors: Peter
authors: 0000-0002-5581-7937
authors: P
authors: Vandamme
mesh_terms: D001530:Belgium; D019117:Burkholderia / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004783:Environmental Microbiology; D006863:Hydrogen-Ion Concentration; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014867:Water / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil; D014867:Water; D011938:Rec A Recombinases
keywords: Burkholderia multivorans; Environmental samples; PCR assay; recA