title: Easy and rapid multi-pass detection of antigen and antibody with micro-lens sensors.
abstract: We introduce a micro-lens imaging method that can perform easy and rapid multi-pass detection of antigen/antibody (Ag/Ab) without the requirement of any labeling, expensive enzymes, pre-immobilization/modification, and post-washing. Our method detects Ag or Ab presenting in solutions in a quantitative or qualitative manner by using micro-lens as the sensor to monitor the refractive index variation of the solutions during the primary stage of Ag-Ab reaction. The detection can be taken rapidly and finished in two minutes, while requires very low sample volume (several micron liters) and its detection limit can be as low as ∼pg/mL. The method is also able to provide kinetic and thermodynamic parameters of Ag-Ab reactions. The detections of ten Ag-Ab systems and two kinds of clinical samples demonstrated that our method is of high sensitivity, accuracy, reliability and permitting on-site analysis.
authors: Department of Biomedical Engineering, Ji Nan University, Guang Zhou, China. Electronic address: tyxhuang@jnu.edu.cn.
authors: Yao-Xiong
authors: YX
authors: Huang
authors: Department of Biomedical Engineering, Ji Nan University, Guang Zhou, China.
authors: Jiang
authors: J
authors: He
authors: Department of Biomedical Engineering, Ji Nan University, Guang Zhou, China.
authors: Chan-Yuan
authors: CY
authors: Tan
authors: Department of Biomedical Engineering, Ji Nan University, Guang Zhou, China.
authors: Man
authors: M
authors: Luo
mesh_terms: D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000276:immunology; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D045424:Complex Mixtures; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D007909:Lenses; D008904:Miniaturization; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014159:Transducers
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D000941:Antigens; D045424:Complex Mixtures
keywords: Detection of antigen/antibody; Micro-lens; Refractive index