title: Identification of Giardia lamblia Assemblage E in Humans Points to a New Anthropozoonotic Cycle.
abstract: Giardia lamblia is a pathogen transmitted by water and food that causes infection worldwide. Giardia genotypes are classified into 8 assemblages (A-H). Assemblages A and B are detected in humans, but they are potentially zoonotic because they infect other mammalian hosts. Giardia in samples from 44 children was genotyped. Conserved fragments of the genes encoding β-giardin and glutamate dehydrogenase were sequenced and their alignment were carried out with sequences deposited in GenBank. As expected for Rio de Janeiro, the majority of samples were related to assemblage A. Surprisingly, assemblage E was detected in 15 samples. Detection of assemblage E in humans suggests a new zoonotic route of Giardia transmission.
authors: Laboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz.
authors: Maria
authors: M
authors: Fantinatti
authors: Disciplina de Parasitologia, DMIP, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil.
authors: Alexandre R
authors: AR
authors: Bello
authors: Laboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz.
authors: Octavio
authors: O
authors: Fernandes
authors: Laboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz Disciplina de Parasitologia, DMIP, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil.
authors: Alda M
authors: AM
authors: Da-Cruz
mesh_terms: D000818:Animals; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000235:genetics; D005838:Genotype; D016829:Giardia lamblia / Q000235:genetics*; D005873:Giardiasis / Q000469:parasitology; D005969:Glutamate Dehydrogenase / Q000235:genetics; D006801:Humans; D007223:Infant; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D005969:Glutamate Dehydrogenase
keywords: Giardia lamblia; assemblage E; molecular characterization; β-giardin gene