title: Molecular detection of Mycobacterium tuberculosis in cattle and buffaloes: a cause for public health concern.
abstract: Tuberculosis is a re-emerging disease causing a growing public health burden. The current study was conducted to investigate the occurrence of Mycobacterium tuberculosis among cattle and buffaloes with tuberculous lesions. Typical tuberculous lesions were collected from 34 cattle and 34 buffaloes (Bubalus bubalis) through postmortem examination of slaughtered animals in abattoirs. DNAs were extracted from samples, and M. tuberculosis was identified by PCR. Positive samples were examined for resistance against rifampicin and isoniazid using GenoType MTBDRplus. Moreover, sera from 90 slaughterhouse workers, butchers, or meat inspectors were examined for the presence of M. tuberculosis antibodies using ELISA. Five cattle (14.7 %) and three buffaloes (8.8 %) tested positive. M. tuberculosis from one cattle was resistant to rifampicin and another was resistant to isoniazid. In addition, the seroprevalence of M. tuberculosis IgG among examined humans was 5.6 %. The occurrence of M. tuberculosis in cattle and buffaloes is a public health concern.
authors: Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, 12211, Egypt. khal_105@cu.edu.eg.
authors: Khaled A
authors: KA
authors: Abdel-Moein
authors: Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, 12211, Egypt.
authors: Osman
authors: O
authors: Hamed
authors: Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, 12211, Egypt.
authors: Heba
authors: H
authors: Fouad
mesh_terms: D000003:Abattoirs; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000097:blood; D002020:Buffaloes / Q000382:microbiology; D002417:Cattle / Q000382:microbiology; D004534:Egypt / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011634:Public Health; D016036:Seroepidemiologic Studies; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology* / Q000517:prevention & control / Q000635:transmission; D015047:Zoonoses / Q000453:epidemiology* / Q000517:prevention & control / Q000635:transmission
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial
keywords: Buffaloes; Cattle; M. tuberculosis; Public health