title: Vitamin C blocks TNF-α-induced NF-kB activation and ICAM-1 expression in human neuroblastoma cells.
abstract: Interactions of the cell adhesion molecules are known to play important roles in mediating inflammation. The proinflammatory cytokine, tumor necrosis factor-α (TNF-α), activates the NF-kB signaling pathway, which induces the expression of various genes, such as intercellular adhesion molecule-1 (ICAM-1). In this study, the effect of vitamin C on the ICAM-1 expression induced by TNF-α in a human neuroblastoma cell line, SK-N-SH was investigated. Treatment with vitamin C resulted in the downregulation of the TNF-α-induced surface expression and ICAM-1 mRNA levels in a concentration-dependent manner. Moreover, a gel shift analysis indicated that vitamin C dose-dependently inhibited the NF-kB activation and lkBα degradation induced by TNF-α. Taken together, these results suggest that vitamin C downregulates TNF-α-induced ICAM-1 expression via the inhibition of NF-kB activation.
authors: Dept. of Pharmacognosy Material Development, Samcheok National University, 245-711, Samcheok, Kangwon-do, Korea.
authors: Eun-Wha
authors: EW
authors: Son
authors: Dept. of Pharmacognosy Material Development, Samcheok National University, 245-711, Samcheok, Kangwon-do, Korea.|Division of Immunopharmacology, College of Pharmacy Sungkyunkwan University, 440-746, Suwon, Kyunggi-do, Korea.
authors: Sung-Ji
authors: SJ
authors: Mo
authors: Dept. of Pharmacognosy Material Development, Samcheok National University, 245-711, Samcheok, Kangwon-do, Korea.|Division of Immunopharmacology, College of Pharmacy Sungkyunkwan University, 440-746, Suwon, Kyunggi-do, Korea.
authors: Dong-Kwon
authors: DK
authors: Rhee
authors: Dept. of Pharmacognosy Material Development, Samcheok National University, 245-711, Samcheok, Kangwon-do, Korea. snpyo@skku.ac.kr.|Division of Immunopharmacology, College of Pharmacy Sungkyunkwan University, 440-746, Suwon, Kyunggi-do, Korea. snpyo@skku.ac.kr.
authors: Suhkneung
authors: S
authors: Pyo
keywords: ICAM-1; NF-kB; TNF-α; Vitamin C