title: Flavonoids stimulate cholecystokinin peptide secretion from the enteroendocrine STC-1 cells.
abstract: Animal experiments showed that flavonoids might have the potential for an anti-obesity effect by reducing weight and food intake. However, the exact mechanisms that could be involved in these proposed effects are still under investigation. The complex process of food intake is partially regulated by gastrointestinal hormones. Cholecystokinin (CCK) is the best known gastrointestinal hormone to induce satiety signal that plays a key role in food intake regulation. It is released from the endocrine cells (I cell) in response to the ingestion of nutrients into the small intestine. In this study, we investigated the possible effects of flavonoids (quercetin, kaempferol, apigenin, rutin and baicalein) on stimulation of CCK release in vitro using enteroendocrine STC-1 cells. In comparison with the control, quercetin, kaempferol and apigenin resulted in a significant increase in CCK secretion with quercetin showing the highest activity. On the other hand, no significant effect was seen by rutin and baicalein. To our knowledge, this is the first report to study the stimulation of CCK peptide hormone secretion from STC-1 cells by quercetin and kaempferol, rutin, apigenin and baicalein. Based on the cell-based results in this work, it can be suggested that the reported activity of flavonoids against food intake and weight could be mediated by stimulation of CCK signal which in turn is responsible for food intake reduction, but future animal and human studies are needed to confirm this conclusion at organism level.
authors: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium; Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium; Department of Industrial Biological Sciences, Faculty of Bioscience Engineering, Ghent University, Campus Kortrijk, Kortrijk, Belgium.
authors: Nadin
authors: N
authors: Al Shukor
authors: Charles Viollette Institute EA7394/USC1281, Université Lille 1, Boulevard Paul Langevin, 59653 Villeneuve d'Ascq Cedex, France.
authors: Rozenn
authors: R
authors: Ravallec
authors: Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
authors: John
authors: J
authors: Van Camp
authors: Department of Industrial Biological Sciences, Faculty of Bioscience Engineering, Ghent University, Campus Kortrijk, Kortrijk, Belgium.
authors: Katleen
authors: K
authors: Raes
authors: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium. Electronic address: guy.smagghe@ugent.be.
authors: Guy
authors: G
authors: Smagghe
mesh_terms: D002460:Cell Line; D002766:Cholecystokinin / Q000378:metabolism*; D019858:Enteroendocrine Cells / Q000187:drug effects* / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans
chemical_list: D005419:Flavonoids; D002766:Cholecystokinin
keywords: Cholecystokinin; Flavonoids; STC-1 cells; food intake