title: The use of epifluorescence microscopy and fluorescent dyes for visualization of Oxyuris equi eggs.
abstract: This study presents a new method for visualization of Oxyuris equi eggs collected by means of a clear adhesive tape applied to the perianal region of horses. The obtained results indicate that this protocol permits a quick, easy, clear and selective visualization of Oxyuris equi eggs even in the presence of feces and other material. Another advantage of this method is that it can be used with fluorescent dyes solubilized in water, which will stain biologic material without dissolving or altering the adhesive tape and is also environmentally safe. Other dyes currently used for staining biologic materials use organic solvents, which may be combined with acids or bases in their formulation, preventing their use with the tape method.
authors: Institute of Chemistry, Laboratory of New Organic Materials and Forensic Chemistry (LNMO-QF), Universidade Federal do Rio Grande do Sul (UFRGS). Av. Bento Gonçalves, 9.500, C.P. 15003, 91.501-970 Porto Alegre (RS), Brazil. Electronic address: h.barros@campus.fct.unl.pt.
authors: Hélio L
authors: HL
authors: Barros
authors: Laboratório de Helmintologia, Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária, UFRGS. Av. Bento Gonçalves, 9090, 91.501-970 Porto Alegre (RS), Brazil. Electronic address: smtmuni@hotmail.com.
authors: Sandra M T
authors: SM
authors: Marques
authors: Institute of Chemistry, Laboratory of New Organic Materials and Forensic Chemistry (LNMO-QF), Universidade Federal do Rio Grande do Sul (UFRGS). Av. Bento Gonçalves, 9.500, C.P. 15003, 91.501-970 Porto Alegre (RS), Brazil. Electronic address: vstefani@iq.ufrgs.br.
authors: Valter
authors: V
authors: Stefani
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000627:therapeutic use; D004355:Drug Stability; D005243:Feces / Q000469:parasitology; D005260:Female; D005273:Fenbendazole / Q000627:therapeutic use; D005456:Fluorescent Dyes / Q000737:chemistry; D006734:Horse Diseases / Q000188:drug therapy / Q000469:parasitology*; D006736:Horses; D008856:Microscopy, Fluorescence; D010063:Ovum / Q000648:ultrastructure; D010123:Oxyuriasis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D010124:Oxyuroidea / Q000254:growth & development*; D012995:Solubility
chemical_list: D000969:Antinematodal Agents; D005456:Fluorescent Dyes; D005273:Fenbendazole
keywords: Benzazole dyes; Biomarkers; Fluorescent probes; Oxyuris equi; Pinworm infection