title: CAND3: A ubiquitously expressed gene immediately adjacent and in opposite transcriptional orientation to the ATM gene at 1lq23.1.
abstract: Using a magnetic beads-mediated cDNA selection procedure and a fetal brain expression library, we identified a transcriptional unit within a cosmid positive for the marker D11S384. Pursuit of its full-length cDNA led to the cloning of the third candidate gene (CAND3) we studied in our quest for the ataxiatelangiectasia (A-T) gene, ATM. CAND3 spans ~140 kb of genomic DNA and is located immediately centrimeric to ATM, with 544 bp of DNA separating the two genes. CAND3 encodes two ubiquitously expressed transcripts of ~5.8 kb and ~4.6 kb that are divergently transcribed from a promoter region common to ATM. Nucleotide sequence was determined for one of its alternately spliced transcripts. The predicted protein has 1175 amino acids and is novel in sequence, with only weak homologies to transcriptional factors, nucleoporin protein, and protein kinases, including members of the phosphatidylinositol 3-kinase (PI-3 kinase) family. Although neither homology to ATM nor any mutation of CAND3 in A-T patients has been found, the head-to-head arrangement of CAND3 and ATM, with expression of both housekeeping genes from a common stretch of 544 bp intergenic DNA, suggests a bi-directional promoter possibly for co-regulation of biologically related functions. YACs, BACs, cosmids, and STSs are defined to aid in the further study of this gene.
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: X
authors: X
authors: Chen
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: L
authors: L
authors: Yang
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: N
authors: N
authors: Udar
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: T
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authors: Liang
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: N
authors: N
authors: Uhrhammer
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: S
authors: S
authors: Xu
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: J O
authors: JO
authors: Bay
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: Z
authors: Z
authors: Wang
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: S
authors: S
authors: Dandakar
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: S
authors: S
authors: Chiplunkar
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: I
authors: I
authors: Klisak
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: M
authors: M
authors: Telatar
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: H
authors: H
authors: Yang
authors: Virginia Mason Research Center and the Department of Immunology, University of Washington School of Medicine, 98101, Seattle, Washington, USA.
authors: P
authors: P
authors: Concannon
authors: Department of Pathology, UCLA School of Medicine, 90095 1732, Los Angeles, California, USA.
authors: R A
authors: RA
authors: Gatti