title: Blood-borne miRNA profile-based diagnostic classifier for lung adenocarcinoma.
abstract: Accumulated evidence indicates that various types of miRNA are aberrantly expressed in lung cancer and secreted into the bloodstream. For this study, we constructed a serum diagnostic classifier based on detailed bioinformatics analysis of miRNA profiles from a training cohort of 143 lung adenocarcinoma patients and 49 healthy subjects, resulting in a 20 miRNA-based classifier. Validation performed with an independent cohort of samples from lung adenocarcinoma patients (n = 110), healthy subjects (n = 52), and benign pulmonary disease patients (n = 47) showed a sensitivity of 89.1% and specificity of 94.9%, with an area under the curve value of 0.958. Notably, 90.8% of Stage I lung adenocarcinoma cases were correctly diagnosed. Interestingly, this classifier also detected squamous and large cell lung carcinoma cases at relatively high rates (70.4% and 70.0%, respectively), which appears to be consistent with organ site-dependent miRNA expression in cancer tissues. In contrast, we observed significantly lower rates (0-35%) using samples from 96 cases of cancer in other major organs, with breast cancer the lowest. These findings warrant a future study to realize its clinical application as a part of diagnostic procedures for lung cancers, for which early detection and surgical removal is presently the only hope for eventual cure.
authors: Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Mei Chee
authors: MC
authors: Tai
authors: Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Kiyoshi
authors: K
authors: Yanagisawa
authors: Center for Advanced Medicine and Clinical Research, Nagoya University Hospital, Nagoya, Japan.
authors: Masahiro
authors: M
authors: Nakatochi
authors: Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Naoe
authors: N
authors: Hotta
authors: Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Yasuyuki
authors: Y
authors: Hosono
authors: Department of Thoracic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Koji
authors: K
authors: Kawaguchi
authors: Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Mariko
authors: M
authors: Naito
authors: Department of Respiratory Medicine and Allergy, Tosei General Hospital, Seto, Japan.
authors: Hiroyuki
authors: H
authors: Taniguchi
authors: Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Kenji
authors: K
authors: Wakai
authors: Department of Thoracic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Kohei
authors: K
authors: Yokoi
authors: Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Takashi
authors: T
authors: Takahashi
mesh_terms: D000230:Adenocarcinoma / Q000145:classification* / Q000175:diagnosis* / Q000235:genetics; D000077192:Adenocarcinoma of Lung; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000145:classification* / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs