title: [The reliability of electrochemical enzyme-linked immunosorbent assay for detection of dentin sialophosphoprotein in gingival crevicular fluid].
abstract: OBJECTIVE
  To investigate the reliability of electrochemical enzyme-linked immunosorbent assay(ELISA)for the detection of dentin sialophosphoprotein(DSPP)in gingival crevicular fluid(GCF)of orthodontic patients.
  
  
  METHODS
  GCF were collected from the orthodontic patients, and then the amount of DSPP in GCF were detected by electrochemical ELISA and spectroscopic ELISA respectively. The difference between the results of two methods were analyzed.
  
  
  RESULTS
  In electrochemical ELISA, the second order derivative linear sweep voltammetric peak current was linear with DSPP concentration in the range from 0.25-800.00 ng/L. It was found that the detection limit of the electrochemical method(0.25 ng/L)was much lower than that of the spectroscopic method. The paired-samples rank sum test showed no significant difference between the DSPP concentrations in human GCF samples measured by spectroscopic ELISA procedure and those measured by electrochemical ELISA procedure(P=0.063).
  
  
  CONCLUSIONS
  Electrochemical ELISA is a viable way of detecting DSPP in GCF with higher sensitivity than spectroscopic ELISA.
authors: Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China.
authors: H L
authors: HL
authors: Sha
authors: Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China.
authors: H M
authors: HM
authors: Wang
authors: Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China.
authors: Y X
authors: YX
authors: Bai
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D010750:Phosphoproteins / Q000032:analysis*; D015203:Reproducibility of Results; D012795:Sialoglycoproteins / Q000032:analysis*; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein