title: Silk-Fibroin and Graphene Oxide Composites Promote Human Periodontal Ligament Stem Cell Spontaneous Differentiation into Osteo/Cementoblast-Like Cells.
abstract: Graphene represents one of the most interesting additions to the tissue engineering toolbox. Novel graphene-based composites are required to improve the beneficial graphene properties in terms of tridimensional polymeric structure, conferring a higher mechanical strength and favoring the differentiation of human mesenchymal stem cells. Here, we have demonstrated in a wide range of composite combinations, the successful use of graphene and silk-fibroin constructs for future bioengineering applications in the field of clinical regenerative dentistry using human periodontal ligament stem cells. Our results provide exciting new data for the development of suitable scaffolds that allow good cell engrafting, preservation of cell viability and proliferation, promotion of spontaneous osteoblastic differentiation, and importantly, stimulation of a higher cementum physiological synthesis than using other different available biomaterials.
authors: 1 Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB, University of Murcia , Murcia, Spain .
authors: Mar
authors: M
authors: Vera-Sánchez
authors: 2 Biotechnology Department, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA) , Murcia, Spain .
authors: Salvador
authors: S
authors: Aznar-Cervantes
authors: 3 Cardiology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB, University of Murcia , Murcia, Spain .
authors: Eva
authors: E
authors: Jover
authors: 1 Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB, University of Murcia , Murcia, Spain .
authors: David
authors: D
authors: García-Bernal
authors: 4 School of Dentistry, Faculty of Medicine, University of Murcia , Murcia, Spain .
authors: Ricardo E
authors: RE
authors: Oñate-Sánchez
authors: 3 Cardiology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB, University of Murcia , Murcia, Spain .
authors: Diana
authors: D
authors: Hernández-Romero
authors: 1 Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB, University of Murcia , Murcia, Spain .
authors: Jose M
authors: JM
authors: Moraleda
authors: 4 School of Dentistry, Faculty of Medicine, University of Murcia , Murcia, Spain .
authors: Mar
authors: M
authors: Collado-González
authors: 1 Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB, University of Murcia , Murcia, Spain .|4 School of Dentistry, Faculty of Medicine, University of Murcia , Murcia, Spain .
authors: Francisco Javier
authors: FJ
authors: Rodríguez-Lozano
authors: 2 Biotechnology Department, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA) , Murcia, Spain .
authors: Jose Luis
authors: JL
authors: Cenis
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology*; D002113:Calcification, Physiologic / Q000187:drug effects / Q000235:genetics; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics; D016923:Cell Death / Q000187:drug effects / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D048430:Cell Shape / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D003739:Dental Cementum / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005349:Fibroins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006108:Graphite / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology*; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D001672:Biocompatible Materials; D006108:Graphite; D005349:Fibroins
keywords: cementoblastic differentiation; graphene; human periodontal ligament stem cells; silk fibroin