title: Chitosan Hydrogel in combination with Nerolidol for healing wounds.
abstract: Chitosan is a natural polymer with antibacterial property, that is biodegradable, extremely abundant and non-toxic. This study aimed to develop and characterize chitosan hydrogels in combination with nerolidol, in order to optimize the antimicrobial and healing properties. The hydrogels were prepared using a reaction of the chitosan with acetic acid solution, followed by the addition of 2 or 4% of the nerolidol. Using thermogravimetry, differential scanning calorimetry and infrared spectroscopy, the incorporation of nerolidol in the hydrogel was confirmed. Direct contact tests using hydrogels and Staphylococcus aureus showed a synergistic effect in the materials, enabling total inhibition of bacterial growth. The hydrogel containing 2% nerolidol showed excellent healing effects. The beginning of re-epithelialization and reorganization of collagen was already observed on the 7th day of treatment. The material created proofed to be promising as a healing and antibacterial agent.
authors: Graduate Program in Materials Science, Federal University of Piauí. Campus Universitário Ministro Petrônio Portella, Teresina, PI CEP 64049-550, Brazil.
authors: Maria Onaira Gonçalves
authors: MOG
authors: Ferreira
authors: Graduate Program in Materials Science, Federal University of Piauí. Campus Universitário Ministro Petrônio Portella, Teresina, PI CEP 64049-550, Brazil.
authors: Layara Lorrana Ribeiro
authors: LLR
authors: Leite
authors: Graduate Program in Materials Science, Federal University of Piauí. Campus Universitário Ministro Petrônio Portella, Teresina, PI CEP 64049-550, Brazil.
authors: Idglan Sá
authors: IS
authors: de Lima
authors: Microbiology Research Laboratory, Federal University of Piauí. Campus Universitário Ministro Petrônio Portella, Teresina, PI CEP 64049-550, Brazil.
authors: Humberto Medeiros
authors: HM
authors: Barreto
authors: Graduate Program in Materials Science, Federal University of Piauí. Campus Universitário Ministro Petrônio Portella, Teresina, PI CEP 64049-550, Brazil.
authors: Lívio César Cunha
authors: LCC
authors: Nunes
authors: Program of Scientific and Regional Development-CNPq, Federal University of Piauí. Campus Universitário Ministro Petrônio Portella, Teresina, PI CEP 64049-550, Brazil. Electronic address: alessandra.bragaribeiro@gmail.com.
authors: Alessandra Braga
authors: AB
authors: Ribeiro
authors: Graduate Program in Materials Science, Federal University of Piauí. Campus Universitário Ministro Petrônio Portella, Teresina, PI CEP 64049-550, Brazil.
authors: Josy Anteveli
authors: JA
authors: Osajima
authors: Graduate Program in Materials Science, Federal University of Piauí. Campus Universitário Ministro Petrônio Portella, Teresina, PI CEP 64049-550, Brazil.
authors: Edson Cavalcanti
authors: EC
authors: da Silva Filho
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D051379:Mice; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000254:growth & development*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D012717:Sesquiterpenes; C037055:nerolidol
keywords: Bactericide; Cicatrization; Dressing; Drug; Gel; Healing; Injury