title: Phylogenetic analysis of the ATP-binding cassette transporter family in three mosquito species.
abstract: The ATP-binding cassette (ABC) transporter family functions in the ATP-dependent transportation of various substrates across biological membranes. ABC proteins participate in various biological processes and insecticide resistance in insects, and are divided into eight subfamilies (A-H). Mosquitoes are important vectors of human diseases, but the mechanism by which the ABC transporter family evolves in mosquitoes is unknown. In this study, we classified and compared the ABC transporter families of three mosquitoes, namely, Anopheles gambiae, Aedes aegypti, and Culex pipiens quinquefasciatus. The three mosquitoes have 55, 69, and 70 ABC genes, respectively. The C. p. quinquefasciatus had approximately 40% and 65% expansion in the ABCG subfamily, mainly in ABCG1/G4, compared with the two other mosquito species. The ABCB, ABCD, ABCE, and ABCF subfamilies were conserved in the three mosquito species. The C. p. quinquefasciatus transcriptomes during development showed that the ABCG and ABCC genes were mainly highly expressed at the egg and pupal stages. The pigment-transport relative brown, white, and scarlet, as well as the ABCF subfamily, were highly expressed at the egg stage. The highly expressed genes in larvae included three ABCA3 genes. The majority of the highly expressed genes in adults were ABCG1/4 genes. These results provided insights into the evolution of the ABC transporter family in mosquitoes.
authors: College of Plant Protection, Shandong Agricultural University, Tai'an, 271018, Shandong, China; State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Hong
authors: H
authors: Lu
authors: College of Plant Protection, Shandong Agricultural University, Tai'an, 271018, Shandong, China.
authors: Yongyu
authors: Y
authors: Xu
authors: State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: cuif@ioz.ac.cn.
authors: Feng
authors: F
authors: Cui
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000330:Aedes / Q000235:genetics*; D000818:Animals; D000852:Anopheles / Q000235:genetics*; D003465:Culex / Q000235:genetics*; D005786:Gene Expression Regulation; D017344:Genes, Insect; D010063:Ovum / Q000378:metabolism; D010802:Phylogeny; D011679:Pupa / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters
keywords: ATP-binding cassette transporter; Aedes aegypti; Anopheles gambiae; Culex pipiens quinquefasciatus; Phylogenetics; Transcriptome