title: Glucose oxidase probe as a surface-enhanced Raman scattering sensor for glucose.
abstract: Glucose oxidase (GOx) possessing a Raman-active chromophore (flavin adenine dinucleotide) is used as a signal reporter for constructing a highly specific "turn off" surface-enhanced Raman scattering (SERS) sensor for glucose. This sensing chip is made by the electrostatic assembly of GOx over silver nanoparticle (Ag NP)-functionalized SERS substrate through a positively charged polyelectrolyte linker under the pH of 6.86. To trace glucose in blood serum, owing to the reduced pH value caused by the production of gluconic acid in the GOx-catalyzed oxidation reaction, the bonding force between GOx and polyelectrolyte weakens, making GOx drop off from the sensing chip. As a result, the SERS intensity of GOx on the chip decreases along with the concentration of glucose. This glucose SERS sensor exhibits excellent selectivity based on the specific GOx/glucose catalysis reaction and high sensitivity to 1.0 μM. The linear sensing range is 2.0-14.0 mM, which also meets the requirement on the working range of the human blood glucose detection. Using GOx as a probe shows superiority over other organic probes because GOx almost has no toxicity to the biological system. This sensing mechanism can be applied for intracellular in vivo SERS monitoring of glucose in the future. Graphical abstract Glucose oxidase is used as a Raman signal reporter for constructing a highly specific glucose surface-enhanced Raman scattering (SERS) sensor.
authors: State Key laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, 2699 Qianjin Street, Changchun, Jilin, 130012, China.
authors: Guohua
authors: G
authors: Qi
authors: State Key laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, 2699 Qianjin Street, Changchun, Jilin, 130012, China.
authors: Yi
authors: Y
authors: Wang
authors: College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun, Jilin, 130012, China.
authors: Biying
authors: B
authors: Zhang
authors: State Key laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, 2699 Qianjin Street, Changchun, Jilin, 130012, China.
authors: Dan
authors: D
authors: Sun
authors: State Key laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, 2699 Qianjin Street, Changchun, Jilin, 130012, China.
authors: Cuicui
authors: C
authors: Fu
authors: State Key laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, 2699 Qianjin Street, Changchun, Jilin, 130012, China.
authors: Weiqing
authors: W
authors: Xu
authors: State Key laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, 2699 Qianjin Street, Changchun, Jilin, 130012, China. xusp@jlu.edu.cn.
authors: Shuping
authors: S
authors: Xu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005942:Gluconates / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D005942:Gluconates; D012834:Silver; D005949:Glucose Oxidase; C030691:gluconic acid
keywords: Biosensor; Glucose; Glucose oxidase; SERS; Static force