title: A comprehensive evaluation of three microfluidic chemiluminescence methods for the determination of the total phenolic contents in fruit juices.
abstract: Three recently reported microfluidic chemiluminescence (MF-CL) methods (based on reactions with acidic permanganate enhanced by formaldehyde (KMnO4-COH), acidic cerium (IV) and rhodamine B (Ce-RB), and acidic cerium (IV) and rhodamine 6G (Ce-R6G) enhanced by SDS) for the determination of the total phenolic content (TPC) in juices were critically evaluated in terms of their selectivity. The evaluation was carried out using 86 analytes, including 22 phenolic compounds (phenolic acids and polyphenols), 6 known non-phenolic antioxidants, 9 amino acids and a number of proteins, carbohydrates, nucleotide bases, inorganic salts and other compounds. Each method was sensitive toward phenolic compounds (PCs). However, the KMnO4-COH CL system showed a higher sensitivity toward phenolic acids and also responded to non-phenolic antioxidants. The other two systems showed higher sensitivity toward polyphenolic compounds than to phenolic acids and did not responded to all other compounds including non-phenolic antioxidants.
authors: Department of Chemistry, College of Science, Sultan Qaboos University, Box 36, Al-Khod 123, Oman.
authors: Buthaina
authors: B
authors: Al Haddabi
authors: Department of Chemistry, College of Science, Sultan Qaboos University, Box 36, Al-Khod 123, Oman. Electronic address: haiderl@squ.edu.om.
authors: Haider A J
authors: HAJ
authors: Al Lawati
authors: Department of Chemistry, College of Science, Sultan Qaboos University, Box 36, Al-Khod 123, Oman.
authors: FakhrEldin O
authors: FO
authors: Suliman
mesh_terms: D000975:Antioxidants / Q000032:analysis; D002957:Citrus / Q000737:chemistry; D000067030:Fruit and Vegetable Juices / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D056656:Lab-On-A-Chip Devices; D049449:Luminescence; D008163:Luminescent Measurements; D029561:Lythraceae / Q000737:chemistry; D044085:Microfluidics; D010636:Phenols / Q000032:analysis*; D059808:Polyphenols / Q000032:analysis; D012235:Rhodamines / Q000032:analysis*
chemical_list: D000975:Antioxidants; D010636:Phenols; D059808:Polyphenols; D012235:Rhodamines; C026188:rhodamine 6G; C029773:rhodamine B
keywords: Chemiluminescence; Interferences; Microfluidics; Phenolic antioxidants; Potassium permanganate; Rhodamine