title: Production of human blood group B antigen epitope conjugated protein in Escherichia coli and utilization of the adsorption blood group B antibody.
abstract: BACKGROUND
  In the process of ABO-incompatible (ABOi) organ transplantation, removal of anti-A and/or B antibodies from blood plasma is a promising method to overcome hyperacute rejection and allograft loss caused by the immune response between anti-A and/or B antibodies and the A and/or B antigens in the recipient. Although there are commercial columns to do this work, the application is still limited because of the high production cost.
  
  
  RESULTS
  In this study, the PglB glycosylation pathway from Campylobacter jejuni was exploited to produce glycoprotein conjugated with Escherichia coli O86:B7 O-antigen, which bears the blood group B antigen epitope to absorb blood group B antibody in blood. The titers of blood group B antibody were reduced to a safe level without changing the clotting function of plasma after glycoprotein absorption of B antibodies in the plasma.
  
  
  CONCLUSIONS
  We developed a feasible strategy for the specific adsorption/removal of blood group antibodies. This method will be useful in ABOi organ transplantation and universal blood transfusion.
authors: The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, School of Life Sciences and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, Shandong, 250100, People's Republic of China.|The Institute of Medical Molecular Genetics, Department of Biochemistry and Molecular Biology, Bin Zhou Medical University, No. 346, Guan Hai Road, Lai Shan District, Yan Tai City, Shan Dong Province, 264003, People's Republic of China.
authors: Wenjing
authors: W
authors: Shang
authors: The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, School of Life Sciences and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, Shandong, 250100, People's Republic of China.
authors: Yafei
authors: Y
authors: Zhai
authors: The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, School of Life Sciences and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, Shandong, 250100, People's Republic of China.
authors: Zhongrui
authors: Z
authors: Ma
authors: The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, School of Life Sciences and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, Shandong, 250100, People's Republic of China.
authors: Gongjin
authors: G
authors: Yang
authors: The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, School of Life Sciences and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, Shandong, 250100, People's Republic of China.
authors: Yan
authors: Y
authors: Ding
authors: The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, School of Life Sciences and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, Shandong, 250100, People's Republic of China.
authors: Donglei
authors: D
authors: Han
authors: The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, School of Life Sciences and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, Shandong, 250100, People's Republic of China.
authors: Jiang
authors: J
authors: Li
authors: The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, School of Life Sciences and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, Shandong, 250100, People's Republic of China.
authors: Houcheng
authors: H
authors: Zhang
authors: The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, School of Life Sciences and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, Shandong, 250100, People's Republic of China.
authors: Jun
authors: J
authors: Liu
authors: The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, School of Life Sciences and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, Shandong, 250100, People's Republic of China.
authors: Peng George
authors: PG
authors: Wang
authors: The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, School of Life Sciences and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, Shandong, 250100, People's Republic of China. xianweiliu@sdu.edu.cn.
authors: Xian-Wei
authors: XW
authors: Liu
authors: The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, School of Life Sciences and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, Shandong, 250100, People's Republic of China. chenmin@sdu.edu.cn.
authors: Min
authors: M
authors: Chen
mesh_terms: D000017:ABO Blood-Group System; D000327:Adsorption; D000906:Antibodies / Q000097:blood; D001777:Blood Coagulation; D001789:Blood Group Antigens / Q000737:chemistry* / Q000276:immunology*; D001803:Blood Transfusion; D016123:Campylobacter jejuni / Q000737:chemistry / Q000235:genetics; D000939:Epitopes; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D019081:O Antigens / Q000737:chemistry*; D016377:Organ Transplantation; D014184:Transplantation, Homologous
chemical_list: D000017:ABO Blood-Group System; D000906:Antibodies; D001789:Blood Group Antigens; D000939:Epitopes; D006023:Glycoproteins; D019081:O Antigens
keywords: Blood group B antigen; Conjugated glycoprotein; E. coli O-antigen; Immunoadsorption; PglB