title: Eccentric Contraction-Induced Muscle Injury: Reproducible, Quantitative, Physiological Models to Impair Skeletal Muscle's Capacity to Generate Force.
abstract: In order to investigate the molecular and cellular mechanisms of muscle regeneration an experimental injury model is required. Advantages of eccentric contraction-induced injury are that it is a controllable, reproducible, and physiologically relevant model to cause muscle injury, with injury being defined as a loss of force generating capacity. While eccentric contractions can be incorporated into conscious animal study designs such as downhill treadmill running, electrophysiological approaches to elicit eccentric contractions and examine muscle contractility, for example before and after the injurious eccentric contractions, allows researchers to circumvent common issues in determining muscle function in a conscious animal (e.g., unwillingness to participate). Herein, we describe in vitro and in vivo methods that are reliable, repeatable, and truly maximal because the muscle contractions are evoked in a controlled, quantifiable manner independent of subject motivation. Both methods can be used to initiate eccentric contraction-induced injury and are suitable for monitoring functional muscle regeneration hours to days to weeks post-injury.
authors: Department of Kinesiology, University of Georgia, Athens, GA, 30602, USA.|Regenerative Bioscience Center, University of Georgia, Athens, GA, 30602, USA.
authors: Jarrod A
authors: JA
authors: Call
authors: Programs in Physical Therapy and Rehabilitation Science, Department of Physical Medicine and Rehabilitation Medical School, University of Minnesota, Minneapolis, MN, 55455, USA. lowex017@umn.edu.
authors: Dawn A
authors: DA
authors: Lowe
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D051379:Mice; D023421:Models, Animal; D008954:Models, Biological; D009119:Muscle Contraction; D053580:Muscle Strength; D018482:Muscle, Skeletal / Q000293:injuries* / Q000503:physiopathology
keywords: Force drop; Lengthening contraction; Muscle damage