title: 3D origami electrochemical device for sensitive Pb2+testing based on DNA functionalized iron-porphyrinic metal-organic framework.
abstract: A highly sensitive electrochemical (EC) biosensor combined with a 3D origami device for detection of Pb2+was developed based on novel Au nanoparticles modified paper working electrode (Au-PWE) as sensor platform and DNA functionalized iron-porphyrinic metal-organic framework ((Fe-P)n-MOF-Au-GR) hybrids as signal probes. In the presence of Pb2+, GR could be specifically cleaved at the ribonucleotide (rA) site, which produced the short (Fe-P)n-MOF-linked oligonucleotide fragment to hybridize with hairpin DNA immobilized on the surface of Au-PWE. Because of the mimic peroxidase property of (Fe-P)n-MOF, enzymatically amplified electrochemical signal was obtained to offer the sensitive detection of Pb2+. In addition, benefiting from the Pb2+dependent GR, the proposed assay could selectively detect Pb2+in the presence of other metal ions. This method showed a good linear relationship between the current response and the Pb2+concentration ranging from 0.03 to 1000nmolL-1with a detection limit of 0.02nmolL-1. The Au-PWE based electrochemical sensor along with the (Fe-P)n-MOF-Au-GR probe exhibited the advantages of low-cost, simple fabrication, high sensitivity and selectivity, providing potential application of real-time Pb2+detection both in environmental and biological samples.
authors: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors: Xiu
authors: X
authors: Wang
authors: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors: Chunlei
authors: C
authors: Yang
authors: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors: Shaojun
authors: S
authors: Zhu
authors: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: chm_yanm@126.com.
authors: Mei
authors: M
authors: Yan
authors: Shandong Provincial Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, PR China.
authors: Shenguang
authors: S
authors: Ge
authors: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors: Jinghua
authors: J
authors: Yu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004566:Electrodes; D004784:Environmental Monitoring / Q000295:instrumentation; D006046:Gold / Q000737:chemistry; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000737:chemistry*; D007501:Iron / Q000737:chemistry; D007854:Lead / Q000032:analysis* / Q000097:blood*; D057230:Limit of Detection; D010209:Paper; D011166:Porphyrins / Q000737:chemistry; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000097:blood*
chemical_list: D061925:Immobilized Nucleic Acids; D011166:Porphyrins; D014874:Water Pollutants, Chemical; D014867:Water; D007854:Lead; D006046:Gold; D007501:Iron
keywords: (Fe-P)(n)-MOF-Au-GR; 3D origami electrochemical device; Pb(2+) detection