title: Microstructural, mechanical, and histological evaluation of modified alginate-based scaffolds.
abstract: Scaffolds are three-dimensional structures used for tissue regeneration being the base in tissue engineering. These scaffolds are obtained from natural and/or synthetic polymers and they should satisfy some specific requirements such as biocompatibility, suitable mechanical, and microstructural properties to favor cellular adhesion and neovascularization. This work shows a preclinic study about the production of low and medium molecular weight alginate through the use of calcium salts (calcium glutamate). The results showed prove that better structures, distribution, and pore sizes as well as better mechanical properties correspond to medium molecular weight alginate and higher calcium salts concentration. This type of scaffold, after muscular cells cultivation, has been proved as an excellent material for muscle growth. The histopathological analysis shows a low inflammatory response, without a foreign body reaction, suitable neovascularization and good fibroblasts incorporation. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 3107-3114, 2016.
authors: Department of General and Digestive Surgery, Unit Colorrectal Surgery, "Virgen del Rocío" University Hospital/IBiS/CSIC/University of Seville, Seville, Spain.|Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD o Ciberehd), Instituto de Salud Carlos III, Spain.
authors: F
authors: F
authors: de la Portilla
authors: Institute of Biomedicine of Seville (IBiS), "Virgen del Rocío" University Hospital/IBiS/CSIC/University of Seville, Seville, Spain.
authors: S
authors: S
authors: Pereira
authors: Department of Physical Chemistry, Faculty of Chemistry, University of Seville, Sevilla, Spain.
authors: M
authors: M
authors: Molero
authors: Institute of Biomedicine of Seville (IBiS), "Virgen del Rocío" University Hospital/IBiS/CSIC/University of Seville, Seville, Spain.
authors: F
authors: F
authors: De Marco
authors: Department of Chemical Engineering, Faculty of Chemistry, University of Seville, Sevilla, Spain.
authors: V
authors: V
authors: Perez-Puyana
authors: Department of Chemical Engineering, Faculty of Chemistry, University of Seville, Sevilla, Spain.
authors: A
authors: A
authors: Guerrero
authors: Department of Chemical Engineering, Faculty of Chemistry, University of Seville, Sevilla, Spain. alromero@us.es.
authors: A
authors: A
authors: Romero
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D002460:Cell Line; D019245:Compressive Strength; D005260:Female; D020723:Glucuronic Acid / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D032342:Muscle Cells / Q000166:cytology* / Q000637:transplantation; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D018698:Glutamic Acid; D020723:Glucuronic Acid; D002118:Calcium
keywords: alginate; histology; mechanical properties; microstructure; scaffolds