title: Olive Oil-Based Lipid Emulsions Do Not Influence Platelet Receptor Expression in Comparison to Medium and Long Chain Triglycerides In vitro.
abstract: Lipid emulsions influence platelet aggregation and receptor expression. However, the effect on platelet function is not fully explained. Therefore, the aim of this study was to examine the influence of the lipids Lipofundin®, Lipidem®and ClinOleic®on surface expressions of P-selectin, GPIb and GPIIb/IIIa on platelets in vitro. Whole blood was incubated in two different concentrations (0.06 and 0.6 mg/ml) of LCT/MCT, n-3/LCT/MCT and LCT-MUFA for 30 min, followed by activation with TRAP-6 or ADP for flow-cytometric assay. Rates of P-selectin, GPIb and GPIIb/IIIa expression were analyzed. There was a significant increase in GPIIb/IIIa- and P-selectin-expression after incubation with LCT/MCT and n-3/LCT/MCT at the concentration of 0.6 mg/ml, without and after stimulation with TRAP-6 and ADP. GPIb was significantly decreased. Accordingly, LCT-MUFA had no effect on receptor expression of platelets in vitro. We demonstrated that LCT-MUFA did not activate receptor expression of platelets whereas LCT/MCT significantly increased platelet aggregation in vitro. This finding should be noted for parenteral nutrition of intensive care patients and, in the future, might provide further insight into the pathogenic pathways of acute thromboembolic events. However, prospectively designed clinical studies are needed to support our results.
authors: Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg Strasse 1, 30625, Hannover, Germany. stoetzer.carsten@mh-hannover.de.
authors: Carsten
authors: C
authors: Stoetzer
authors: Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg Strasse 1, 30625, Hannover, Germany.
authors: Katja
authors: K
authors: Nickel
authors: Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg Strasse 1, 30625, Hannover, Germany.
authors: Annette
authors: A
authors: Weißig
authors: Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg Strasse 1, 30625, Hannover, Germany.
authors: Marieke
authors: M
authors: Großheim
authors: Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg Strasse 1, 30625, Hannover, Germany.
authors: Dirk
authors: D
authors: Scheinichen
authors: Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg Strasse 1, 30625, Hannover, Germany.
authors: Thorben
authors: T
authors: Doll
authors: Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg Strasse 1, 30625, Hannover, Germany.
authors: Björn
authors: B
authors: Jüttner
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004338:Drug Combinations; D005217:Fat Emulsions, Intravenous / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D000069463:Olive Oil / Q000737:chemistry / Q000494:pharmacology; D019007:P-Selectin / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism*; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*; D013012:Sorbitol / Q000737:chemistry / Q000494:pharmacology*; D013024:Soybean Oil / Q000737:chemistry / Q000494:pharmacology*; D014280:Triglycerides / Q000737:chemistry / Q000494:pharmacology
chemical_list: C568346:ClinOleic; D004338:Drug Combinations; D005217:Fat Emulsions, Intravenous; D000069463:Olive Oil; D019007:P-Selectin; D010743:Phospholipids; D010938:Plant Oils; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019038:Platelet Glycoprotein GPIb-IX Complex; C546937:SELP protein, human; D014280:Triglycerides; C008288:Lipofundin; D013012:Sorbitol; D013024:Soybean Oil
keywords: Flow cytometry; Lipid emulsions; Parenteral nutrition; Platelets