title: MiR-338-3p targets pyruvate kinase M2 and affects cell proliferation and metabolism of ovarian cancer.
abstract: MiR-338-3p is down-regulated in cancer, which inhibits cancer cell proliferation, metastasis, and increases chemosensitivity, but its functions in ovarian cancer remains unknown. The present study aims to identify the miR-338-3p targeted genes and to investigate the associated regulatory mechanisms in ovarian cancer cell proliferation and metabolism. Our results demonstrated miR-338-3p expression was down-regulated in most of ovarian cancer tissues and cell lines. Restoration of miR-338-3p expression in ovarian cancer cells could inhibit cell proliferation, lactate production and lactate production of ovarian cancer cells. PKM2 was verified as a target gene of miR-338-3p by luciferase assay. Further study indicated miR-338-3p controlled ovarian cancer cell metabolism by inhibiting PKM2 expression. It is summarized that the regulatory role of miR-338-3p on PKM2 expression in ovarian cancer may play important roles in cell metabolism.
authors: Department of Gynaecology and Obstetrics, University-Town Hospital of Chongqing Medical University Chongqing, China.
authors: Yuting
authors: Y
authors: Zhang
authors: Department of Digestive Diseases, University-Town Hospital of Chongqing Medical University Chongqing, China.
authors: Bing
authors: B
authors: Shi
authors: Department of Hepatobiliary Surgery, Guiyang Hospital of Guizhou Aviation Industry Group Guiyang, China.
authors: Jiang
authors: J
authors: Chen
authors: Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Chongqing Medical University Chongqing, China.
authors: Lina
authors: L
authors: Hu
authors: Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Chongqing Medical University Chongqing, China.
authors: Chunquan
authors: C
authors: Zhao
keywords: MiR-338-3p; PKM2; metabolism; ovarian cancer; proliferation