title: Anti-obesity effects of boiled tuna extract in mice with obesity induced by a high-fat diet.
abstract: The aim of this study was to examine the anti-obesity effects of boiled tuna extract in C57BL/6N mice with obesity induced by a high-fat diet (HFD). We determined the anti-obesity effects of boiled tuna extract (100, 200, or 400 mg/kg) on the progression of HFD-induced obesity for 10 weeks. The mice were divided into 5 groups as follows: the normal diet (ND) group (n=10); the HFD group (n=10); the mice fed HFD and 100 mg/kg boiled tuna extract group (n=10); those fed a HFD and 200 mg/kg boiled tuna extract group (n=10); and those fed a HFD and 400 mg/kg boiled tuna extract group (n=10). Changes in body weight, fat content, serum lipid levels and lipogenic enzyme levels were measured. The consumption of boiled tuna extract lowered epididymal tissue weight and exerted anti-obesity effects, as reflected by the serum glucose, triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL‑C), low-density lipoprotein cholesterol (LDL-C), insulin and leptin levels. In addition, we demonstrated changes in liver adipogenic- and lipogenic-related protein expression by western blot analysis. Boiled tuna extract downregulated the levels of the CCAAT/enhancer-binding protein α, β and δ (C/EBPα, β, δ), and peroxisome proliferator-activated receptor-γ (PPAR-γ) adipocyte marker genes. Boiled tuna extract also attenuated adipogenic and lipogenic gene expression, namely the levels of fatty acid synthase (FAS), lipoprotein lipase (LPL), acetyl-CoA carboxylase (ACC), glucose transporter type 4 (Glut4) and phosphorylated adenosine monophosphate-activated protein kinase α and β (AMPKα, β) in a dose-dependent manner. Moreover, the consumption of boiled tuna extract restored the levels of superoxide dismutase (SOD), catalase (CAT), glutamic oxaloacetic transaminase (GOT), glutamic-pyruvate transaminase (GPT), aspartate transaminase (AST) and alanine transaminase (ALT) to those of the control group. These results suggest that boiled tuna extract attenuates the progression of obesity by stimulating fatty acid oxidation through the upregulation of AMPK genes, as well as by inhibiting the synthesis of adipogenic and lipogenic enzymes. These characteristics of boiled tuna extract highlight its potential anti-obesity effects.
authors: Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Youngmin
authors: Y
authors: Kim
authors: Institute of Fisheries Science, Pukyong National University, Busan 619‑911, Republic of Korea.
authors: Mi-Jin
authors: MJ
authors: Kwon
authors: Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Jeong-Wook
authors: JW
authors: Choi
authors: Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Min-Kyeong
authors: MK
authors: Lee
authors: Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Chorong
authors: C
authors: Kim
authors: Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Jaehun
authors: J
authors: Jung
authors: Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Heny
authors: H
authors: Aprianita
authors: Dongwon F&B, Seoul 06775, Republic of Korea.
authors: Heesop
authors: H
authors: Nam
authors: Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Taek-Jeong
authors: TJ
authors: Nam
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000273:Adipose Tissue / Q000187:drug effects / Q000473:pathology; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D045424:Complex Mixtures / Q000494:pharmacology / Q000627:therapeutic use*; D059305:Diet, High-Fat; D005786:Gene Expression Regulation; D006358:Hot Temperature; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D050155:Lipogenesis / Q000187:drug effects / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics; D009929:Organ Size / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D014413:Tuna / Q000378:metabolism*
chemical_list: D019440:Anti-Obesity Agents; D001786:Blood Glucose; D045424:Complex Mixtures; D007328:Insulin; D020738:Leptin; D014280:Triglycerides; D002374:Catalase; D013482:Superoxide Dismutase; D000637:Transaminases; C036402:glutamate aminotransferase