title: Antibody to CMRF35-Like Molecule 2, CD300e A Novel Biomarker Detected in Patients with Fulminant Type 1 Diabetes.
abstract: OBJECTIVE
  Fulminant type 1 diabetes (FT1D) is a distinct subtype of type 1 diabetes and is fatal without immediate diagnosis and treatment. At present, there are no biomarkers for early and predictive detection of FT1D.
  
  
  METHODS
  First, we analyzed a total of 6 serum samples from 3 patients with FT1D (1 sample in the acute and 1 in the sub-acute phases from each patient) by seromic analysis. Second, titres of the antibody were measured by ELISA in sera from 30 patients with FT1D (both in the acute and sub-acute phases), 13 patients with FT1D in the chronic phase, 32 patients with autoimmune type 1 (type 1A) diabetes (T1AD), 30 patients with type 2 diabetes (T2D), 23 patients with autoimmune thyroid disease (AITD) and 31 healthy control subjects (HC).
  
  
  RESULTS
  Seromic analysis revealed 9 antibodies which showed high signals from all 3 patients with FT1D in the acute phase. Among them, the titre of anti-CD300e antibody was significantly higher in FT1D patients in the acute phase than that in T1AD, T2D, AITD patients and HC, as determined by ELISA (P<0.01, respectively). The titre of anti-CD300e antibody was also higher in FT1D in the acute phase than that in the sub-acute phase (P = 0.0018, Wilcoxon signed-rank test). The titre of anti-LGALS3 antibody in FT1D patients in the acute phase did not differ from that in patients with FT1D in the sub-acute phase, T1AD, T2D, AITD and HC.
  
  
  CONCLUSIONS
  The titre of a novel antibody, anti-CD300e, was high in sera from patients with FT1D. This antibody might be a diagnostic marker and provide new insight into the pathogenesis of FT1D.
authors: Department of Internal Medicine (I), Osaka Medical College, Takatsuki, Japan.
authors: Fumitaka
authors: F
authors: Haseda
authors: Department of Internal Medicine (I), Osaka Medical College, Takatsuki, Japan.|Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Japan.
authors: Akihisa
authors: A
authors: Imagawa
authors: Experimental Immunology, Immunology Frontier Research Center, Osaka University, Suita, Japan.|Division of Cancer Immunology, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Japan.
authors: Hiroyoshi
authors: H
authors: Nishikawa
authors: Department of Internal Medicine (I), Osaka Medical College, Takatsuki, Japan.
authors: Shinobu
authors: S
authors: Mitsui
authors: Department of Internal Medicine (I), Osaka Medical College, Takatsuki, Japan.
authors: Chiharu
authors: C
authors: Tsutsumi
authors: Department of Internal Medicine (I), Osaka Medical College, Takatsuki, Japan.
authors: Reiko
authors: R
authors: Fujisawa
authors: Department of Internal Medicine (I), Osaka Medical College, Takatsuki, Japan.
authors: Hiroyuki
authors: H
authors: Sano
authors: Department of Internal Medicine (I), Osaka Medical College, Takatsuki, Japan.
authors: Yuko
authors: Y
authors: Murase-Mishiba
authors: Department of Internal Medicine (I), Osaka Medical College, Takatsuki, Japan.
authors: Jungo
authors: J
authors: Terasaki
authors: Experimental Immunology, Immunology Frontier Research Center, Osaka University, Suita, Japan.
authors: Shimon
authors: S
authors: Sakaguchi
authors: Department of Internal Medicine (I), Osaka Medical College, Takatsuki, Japan.
authors: Toshiaki
authors: T
authors: Hanafusa
mesh_terms: D000328:Adult; D000954:Antigens, Surface / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D008875:Middle Aged; D012372:ROC Curve
chemical_list: D000954:Antigens, Surface; D001323:Autoantibodies; D015415:Biomarkers; C444139:CD300C protein, human; D008562:Membrane Glycoproteins