title: HC-Pro viral suppressor from tobacco vein banding mosaic virus interferes with DNA methylation and activates the salicylic acid pathway.
abstract: Salicylic acid (SA) is an important signalling molecule that is synthesized by plants and induces the expression of resistance genes. The SA pathway is typically activated by DNA viruses as well as RNA viruses. Here, we demonstrated that heper-component protease (HC-Pro) encoded by tobacco vein banding mosaic virus (TVBMV) decreases in DNA methylation at the promoters of the regulators ACD6 and NPR1 in the SA pathway. We found that the overexpression of HC-Pro increases the expression of components in the SA pathway in plants. The results revealed that HC-Pro interferes in DNA methylation and activates the SA pathway in the HC-Pro transgenic plants and TVBMV-infected plants. We further found that the accumulation of siRNAs derived from the promoter repeats of ACD6 and NPR1 is greatly reduced in the HC-Pro plants. Our results suggested that HC-Pro-mediated interference with DNA methylation is likely caused by a reduction in accumulation of siRNAs.
authors: School of Life Sciences, Jilin Normal University, Siping 136000, China. Electronic address: yangliping781124@163.com.
authors: Liping
authors: L
authors: Yang
authors: Key Laboratory for Plant Resources Science and Green Production, Jilin Normal University, Siping, Jilin 136000, China.
authors: Yanan
authors: Y
authors: Xu
authors: School of Life Sciences, Jilin Normal University, Siping 136000, China.
authors: Yuqing
authors: Y
authors: Liu
authors: School of Life Sciences, Jilin Normal University, Siping 136000, China.
authors: Dawei
authors: D
authors: Meng
authors: School of Life Sciences, Jilin Normal University, Siping 136000, China. Electronic address: yangliping781124@163.com.
authors: Taicheng
authors: T
authors: Jin
authors: Key Laboratory for Plant Resources Science and Green Production, Jilin Normal University, Siping, Jilin 136000, China. Electronic address: yangliping781124@163.com.
authors: Xiaofu
authors: X
authors: Zhou
mesh_terms: D003546:Cysteine Endopeptidases / Q000378:metabolism*; D019175:DNA Methylation; D060467:Disease Resistance / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology*; D030821:Plants, Genetically Modified; D017800:Potyvirus / Q000502:physiology*; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D020156:Salicylic Acid / Q000378:metabolism*; D015398:Signal Transduction; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus; D020156:Salicylic Acid
keywords: HC-Pro; RNA silencing suppressor; SiRNAs; Tobacco vein banding mosaic virus