title: Development of an In Vitro Assay for Detection of Drug-Induced Resuscitation-Promoting-Factor-Dependent Mycobacteria.
abstract: Tuberculosis is a major infectious disease that requires prolonged chemotherapy with a combination of four drugs. Here we present data suggesting that treatment of Mycobacterium tuberculosis, the causative agent of tuberculosis, and Mycobacterium smegmatis, a model organism widely used for the screening of antituberculosis agents, with first-line drugs resulted in the generation of substantial populations that could be recovered only by the addition of a culture supernatant from growing mycobacteria. These bacilli failed to grow in standard media, resulting in significant underestimation of the numbers of viable mycobacteria in treated samples. We generated M. smegmatis strains overexpressing M. tuberculosis resuscitation-promoting factors (Rpfs) and demonstrated their application for the detection of Rpf-dependent mycobacteria generated after drug exposure. Our data offer novel opportunities for validation of the sterilizing activity of antituberculosis agents.
authors: Department of Infection, Immunity, and Inflammation, University of Leicester, Leicester, United Kingdom.
authors: Jessica
authors: J
authors: Loraine
authors: Department of Infection, Immunity, and Inflammation, University of Leicester, Leicester, United Kingdom.
authors: Feifei
authors: F
authors: Pu
authors: Department of Infection, Immunity, and Inflammation, University of Leicester, Leicester, United Kingdom.
authors: Obolbek
authors: O
authors: Turapov
authors: Department of Infection, Immunity, and Inflammation, University of Leicester, Leicester, United Kingdom gvm4@le.ac.uk.
authors: Galina V
authors: GV
authors: Mukamolova
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008826:Microbial Sensitivity Tests / Q000379:methods; D020102:Mycobacterium smegmatis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D015203:Reproducibility of Results
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003470:Culture Media; D016207:Cytokines; C113704:resuscitation-promoting factor, bacteria