title: Increased EZH2 and decreased osteoblastogenesis during local irradiation-induced bone loss in rats.
abstract: Radiation therapy is commonly used to treat cancer patients but exhibits adverse effects, including insufficiency fractures and bone loss. Epigenetic regulation plays an important role in osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Here, we reported local bone changes after single-dose exposure to (137)CS irradiation in rats. Femur bone mineral density (BMD) and trabecular bone volume in the tibia were significantly decreased at 12 weeks after irradiation. Micro-CT results showed that tBMD, Tb.h and Tb.N were also significantly reduced at 12 weeks after irradiation exposure. ALP-positive OB.S/BS was decreased by 42.3% at 2 weeks after irradiation and was decreased by 50.8% at 12 weeks after exposure. In contrast to the decreased expression of Runx2 and BMP2, we found EZH2 expression was significantly increased at 2 weeks after single-dose (137)CS irradiation in BMSCs. Together, our results demonstrated that single-dose (137)CS irradiation induces BMD loss and the deterioration of bone microarchitecture in the rat skeleton. Furthermore, EZH2 expression increased and osteoblastogenesis decreased after irradiation. The underlying mechanisms warrant further investigation.
authors: Shanghai Key Laboratory for the Prevention and Treatment of Bone and Joint Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and Orthopedics, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine. Address: No. 197, Rui Jin Er Road, Shanghai 200025 China.
authors: Changjun
authors: C
authors: Guo
authors: Shanghai Key Laboratory for the Prevention and Treatment of Bone and Joint Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and Orthopedics, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine. Address: No. 197, Rui Jin Er Road, Shanghai 200025 China.
authors: Changwei
authors: C
authors: Li
authors: Shanghai Key Laboratory for the Prevention and Treatment of Bone and Joint Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and Orthopedics, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine. Address: No. 197, Rui Jin Er Road, Shanghai 200025 China.
authors: Kai
authors: K
authors: Yang
authors: Shanghai Key Laboratory for the Prevention and Treatment of Bone and Joint Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and Orthopedics, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine. Address: No. 197, Rui Jin Er Road, Shanghai 200025 China.
authors: Hui
authors: H
authors: Kang
authors: Department of Bone Metabolism, Institute of Radiation Medicine, Fudan University, Shanghai 200032, China. Address: No. 2094, Xietu Road, Shanghai 200032 China.
authors: Xiaoya
authors: X
authors: Xu
authors: Department of Orthopedics, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine. Address: No. 197, Rui Jin Er Road, Shanghai 200025 China.
authors: Xiangyang
authors: X
authors: Xu
authors: Shanghai Key Laboratory for the Prevention and Treatment of Bone and Joint Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and Orthopedics, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine. Address: No. 197, Rui Jin Er Road, Shanghai 200025 China.
authors: Lianfu
authors: L
authors: Deng
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D018488:Bone Demineralization, Pathologic / Q000209:etiology / Q000378:metabolism*; D015519:Bone Density; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D000071221:Enhancer of Zeste Homolog 2 Protein / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D044127:Epigenesis, Genetic; D005269:Femur / Q000000981:diagnostic imaging / Q000528:radiation effects; D008297:Male; D009369:Neoplasms / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism*; D010012:Osteogenesis; D051381:Rats; D013977:Tibia / Q000000981:diagnostic imaging / Q000528:radiation effects; D055114:X-Ray Microtomography
chemical_list: C578253:EZH2 protein, rat; D000071221:Enhancer of Zeste Homolog 2 Protein