title: SCAMP 3 is a novel regulator of endosomal morphology and composition.
abstract: Secretory Carrier Membrane Proteins (SCAMPs) are transmembrane proteins that function in the plasma membrane, endosomes, and trans-Golgi network. Here we show that SCAMP 3 is a novel regulator of endosomal morphology and composition. Under certain nutrient-starved conditions, SCAMP 3 concentrates in enlarged early endosomes. The enlarged contain ubiquitylated and non-ubiquitylated SCAMP 3 as well as other SCAMPs, EEA1, and the ESCRT-0 protein Hrs. We demonstrate that SCAMP 3 is sufficient to recruit Hrs to the enlarged endosomes. Taken together, our results suggest a novel role for SCAMP 3 in modifying endosome structure through interactions that involve its ubiquitylation and ESCRT proteins.
authors: Department of Biology, Gannon University, Erie, PA 16514, United States.
authors: Priscilla
authors: P
authors: Thomas
authors: Department of Biology, Gannon University, Erie, PA 16514, United States.
authors: Dacey
authors: D
authors: Wohlford
authors: Department of Biology, Gannon University, Erie, PA 16514, United States. Electronic address: aoh001@gannon.edu.
authors: Quyen L
authors: QL
authors: Aoh
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D016895:Culture Media, Serum-Free; D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D002352:Carrier Proteins; D016895:Culture Media, Serum-Free; D056827:Endosomal Sorting Complexes Required for Transport; D008565:Membrane Proteins; D010750:Phosphoproteins; C109303:SCAMP3 protein, human; C096164:hepatocyte growth factor-regulated tyrosine kinase substrate; D012710:Serum Albumin, Bovine
keywords: ESCRT; Endosome; SCAMP; Ubiquitin