title: Correlations of Promoter Methylation in WIF-1, RASSF1A, and CDH13 Genes with the Risk and Prognosis of Esophageal Cancer.
abstract: BACKGROUND This study was designed to explore the correlations of promoter methylation in Wnt inhibitory factor-1 (WIF-1), ras-association domain family member 1A (RASSF1A), and Cadherin 13 (CDH13) genes with the risk and prognosis of esophageal cancer (EC). MATERIAL AND METHODS A total of 71 EC tissues from resection and 35 adjacent normal tissues were collected. Methylation status in the promoter region was detected by methylation- and non-methylation-specific primers. Corresponding mRNA levels were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Correlations between the methylations of these 3 genes and clinicopathologic characteristics were analyzed. Kaplan-Meier method and Cox regression model were used to investigate the relationships between WIF-1, RASSF1A, and CDH13 promoter methylations and the prognosis of EC. RESULTS Compared with adjacent normal tissues, the methylation frequencies of WIF-1, RASSF1A, and CDH13 genes were significantly higher but the mRNA levels of these 3 genes were significantly lower in EC tissues (all P<0.05). WIF-1 and CDH13 promoter methylations were associated with the degree of tumor differentiation and WIF-1 and RASSF1A promoter methylations were associated with age (all P<0.05). The survival rates of patients with WIF-1, RASSF1A, and CDH13 methylations were significantly lower than those of patients without methylation (all P<0.05). WIF-1, RASSF1A, and CDH13 promoter methylations were independent risk factors affecting the prognosis of EC (all P<0.05). CONCLUSIONS WIF-1, RASSF1A, and CDH13 promoter methylations are associated with EC. The methylation levels are negatively related with the prognosis in EC.
authors: Department of Thoracic Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei, China (mainland).
authors: Qiang
authors: Q
authors: Guo
authors: Department of Thoracic Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei, China (mainland).
authors: Hai-Bo
authors: HB
authors: Wang
authors: Department of Thoracic Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei, China (mainland).
authors: Yong-Hui
authors: YH
authors: Li
authors: Department of Thoracic Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei, China (mainland).
authors: He-Fei
authors: HF
authors: Li
authors: Department of Thoracic Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei, China (mainland).
authors: Ting-Ting
authors: TT
authors: Li
authors: Department of Thoracic Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei, China (mainland).
authors: Wen-Xue
authors: WX
authors: Zhang
authors: Department of Thoracic Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei, China (mainland).
authors: Sha-Sha
authors: SS
authors: Xiang
authors: Department of Thoracic Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei, China (mainland).
authors: Zhen-Qing
authors: ZQ
authors: Sun
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D000368:Aged; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D012307:Risk Factors; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015820:Cadherins; C099972:H-cadherin; C411762:RASSF1 protein, human; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins; C485569:WIF1 protein, human