title: Genotoxicity reduction in bagasse waste of sugar industry by earthworm technology.
abstract: The aim of the present study was to assess the genotoxicity reduction in post vermicompost feed mixtures of bagasse (B) waste using earthworm Eisenia fetida. The genotoxicity of bagasse waste was determined by using Allium cepa root chromosomal aberration assay. Bagasse was amended with cattle dung in different proportions [0:100 (B0) 25:75 (B25), 50:50 (B50), 75:25 (B75) and 100:0 (B100)] on dry weight basis. Genotoxic effects of initial and post vermicompost bagasse extracts were analysed on the root tips cells of Allium cepa. Root length and mitotic index (MI) was found to be increased in post vermicompost extracts when compared to initial bagasse waste. The maximum percent increase of root length was observed in the B50 bagasse extract (96.60 %) and the maximum MI was observed in B100 mixture (14.20 ± 0.60) 6 h treatment which was similar to the control. Genotoxicity analysis of post vermicompost extracts of bagasse revealed a 21-44 % decline in the aberration frequencies and the maximum reduction was found in B75 extract (44.50 %). The increase in root length and mitotic index, as well as decrease in chromosomal aberrations indicates that E. fetida has the ability to reduce the genotoxicity of the bagasse waste.
authors: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.
authors: Sartaj Ahmad
authors: SA
authors: Bhat
authors: PG Department of Zoology, Khalsa College, Amritsar, Punjab India.
authors: Jaswinder
authors: J
authors: Singh
authors: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.
authors: Adarsh Pal
authors: 0000-0002-6118-5956
authors: AP
authors: Vig
keywords: Allium cepa aberration assay; Eisenia fetida; Industrial waste; Toxicity; Vermicomposting