title: Control of the heat stress-induced alternative splicing of a subset of genes by hnRNP K.
abstract: Pre-mRNA splicing is widely repressed upon heat shock in eukaryotic cells. However, it has been shown that HSP105 pre-mRNA is alternatively spliced in response to heat stress. Using RNAi screening in HeLa cells, we found that RNA-binding proteins hnRNP K and PSF/SFPQ are necessary for the exon 12 exclusion of HSP105 during heat stress. Moreover, exon array analyses showed that a group of genes is alternatively spliced during heat stress in an hnRNP K-dependent manner, whereas hnRNP K is not necessary for the stress-induced alternative splicing of the remaining genes. Among the latter group, we found that SRp38/SRSF10 and SC35/SRSF2 are necessary for the inclusion of exon 13 of TNRC6A during heat stress. Thus, our study clearly showed that several RNA-binding proteins are involved in the splicing regulation in response to heat stress in mammalian cells.
authors: Bio Process Research and Development Laboratories, Kyowa Hakko Kirin Co. Ltd, 100-1 Hagiwara-machi, Takasaki, Gunma, 370-0013, Japan.|Department of Biology, Graduate School of Science, Kobe University, Kobe, 657-8501, Japan.
authors: Koichi
authors: K
authors: Yamamoto
authors: Department of Biology, Graduate School of Science, Kobe University, Kobe, 657-8501, Japan.
authors: Mari T
authors: MT
authors: Furukawa
authors: Department of Biology, Graduate School of Science, Kobe University, Kobe, 657-8501, Japan.|Institute for Comprehensive Medical Science (ICMS), Fujita Health University, Toyoake, Aichi, 470-1192, Japan.
authors: Kazuhiro
authors: K
authors: Fukumura
authors: Department of Biology, Graduate School of Science, Kobe University, Kobe, 657-8501, Japan.
authors: Arisa
authors: A
authors: Kawamura
authors: Department of Biology, Graduate School of Science, Kobe University, Kobe, 657-8501, Japan.
authors: Tomoko
authors: T
authors: Yamada
authors: Japan Advanced Institute of Science and Technology, Nomi, Ishikawa, 923-1292, Japan.
authors: Hitoshi
authors: H
authors: Suzuki
authors: Institute for Genetic Medicine, Hokkaido University, Sapporo, 060-0815, Japan.
authors: Tetsuro
authors: T
authors: Hirose
authors: Department of Biology, Graduate School of Science, Kobe University, Kobe, 657-8501, Japan.
authors: Hiroshi
authors: H
authors: Sakamoto
authors: Department of Biology, Graduate School of Science, Kobe University, Kobe, 657-8501, Japan. kunio@kobe-u.ac.jp.
authors: Kunio
authors: K
authors: Inoue
mesh_terms: D017398:Alternative Splicing; D005091:Exons; D050957:HSP110 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells; D018869:Heat-Shock Response / Q000235:genetics*; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D000072670:PTB-Associated Splicing Factor / Q000235:genetics / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism
chemical_list: D050957:HSP110 Heat-Shock Proteins; C097205:HSPH1 protein, human; D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D009687:Nuclear Proteins; D000072670:PTB-Associated Splicing Factor; D012322:RNA Precursors; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins