title: Bacillus anthracis S-layer protein BslA binds to extracellular matrix by interacting with laminin.
abstract: The Bacillus anthracis S-layer protein, BslA, plays a crucial role in mammalian infection. BslA is required to mediate adherence between host cells and vegetative forms of bacteria and this interaction promotes target organs adherence and blood-brain barrier (BBB) penetration in vivo. This study attempts to identify the potential eukaryotic ligand(s) for B. anthracis BslA protein.
  
  
  
  Biochemical approaches have indicated that the putative host cell ligand(s) for BslA is a surface protein, which is independent of the sugar components for binding to Bs1A. A ligand screening using blot overlays, far Western blots and mass spectrometry analyses revealed that BslA binds to mammalian laminin. ELISA based solid-phase binding assays and surface plasmon resonance assays demonstrated that there were high affinity interactions between BslA(260-652) and laminin. The SPR results also revealed the dissociation constants values of 3.172 × 10(-9)M for the binding of BslA(260-652) to laminin.
  
  
  
  These data demonstrated that laminin is a ligand for BslA.
authors: State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, Beijng, 100071, China. springwyc@163.com.
authors: Yanchun
authors: Y
authors: Wang
authors: State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, Beijng, 100071, China.|School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors: Ying
authors: Y
authors: Wei
authors: State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, Beijng, 100071, China.
authors: Shengling
authors: S
authors: Yuan
authors: State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, Beijng, 100071, China.
authors: Haoxia
authors: H
authors: Tao
authors: State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, Beijng, 100071, China.
authors: Jie
authors: J
authors: Dong
authors: State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, Beijng, 100071, China.
authors: Zhaoshan
authors: Z
authors: Zhang
authors: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors: Wei
authors: W
authors: Tian
authors: State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, Beijng, 100071, China. liucj@nic.bmi.ac.cn.
authors: Chunjie
authors: C
authors: Liu
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001408:Bacillus anthracis / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D019556:COS Cells; D002522:Chlorocebus aethiops; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000382:microbiology*; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D007797:Laminin / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D007797:Laminin; D008024:Ligands; D008562:Membrane Glycoproteins; C055764:S-layer proteins
keywords: Bacillus anthracis; BslA protein; Extracellular matrix; Laminin; Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMM)