title: The near-ideal catalytic property of Candida antarctica lipase A to highly concentrate n-3 polyunsaturated fatty acids in monoacylglycerols via one-step ethanolysis of triacylglycerols.
abstract: Declining quantity/quality of available n-3 polyunsaturated fatty acids (n-3 PUFAs) resources demand innovative technology to concentrate n-3 PUFAs from low quality oils into value-added products/health-beneficial ingredients rich in n-3 PUFAs. This work proposed the catalytic property and specificity of an ideal enzyme required to tackle this task and identified Candida antarctica lipase A (CAL-A) is such a near-ideal enzyme in practice, which concentrates n-3 PUFAs from 25% to 27% in oils to a theoretically closer value 90% in monoacylglycerols (MAGs) via one-step enzymatic ethanolysis. Non-regiospecificity and high non-n-3 PUFAs preference of CAL-A are the catalytic feature to selectively cleave non-n-3 PUFAs in all 3 positions of triacylglycerols (TAGs); while high ethanol/TAGs ratio, low operation temperature and high tolerance to polar ethanol are essential conditions beyond biocatalyst itself. C-13 Nuclear magnetic resonance ((13)C NMR) analysis and competitive factor estimation verified the hypothesis and confirmed the plausible suggestion of catalytic mechanism of CAL-A.
authors: College of Life Science, Fujian Normal University, Fuzhou 350117, China; Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark.
authors: Yongjin
authors: Y
authors: He
authors: Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark.
authors: Jingbo
authors: J
authors: Li
authors: Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark.
authors: Sitharam
authors: S
authors: Kodali
authors: College of Life Science, Fujian Normal University, Fuzhou 350117, China; Engineering Research Center of Industrial Microbiology of Ministry of Education, Fujian Normal University, Fuzhou 350117, China. Electronic address: chenbil@fjnu.edu.cn.
authors: Bilian
authors: B
authors: Chen
authors: Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark.
authors: Zheng
authors: Z
authors: Guo
mesh_terms: D002175:Candida / Q000201:enzymology; D002384:Catalysis; D000431:Ethanol / Q000737:chemistry / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D008049:Lipase / Q000378:metabolism*; D050178:Monoglycerides / Q000737:chemistry / Q000378:metabolism; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism
chemical_list: D015525:Fatty Acids, Omega-3; D005656:Fungal Proteins; D050178:Monoglycerides; D014280:Triglycerides; D000431:Ethanol; D008049:Lipase
keywords: Candida antarctica lipase A (CAL-A); Ethanolysis; Fish oil; Microalgae oil; Monoacylglycerols (MAGs); n-3 polyunsaturated fatty acids (n-3 PUFAs)