title: Transgenic over-expression of slit2 enhances disruption of blood-brain barrier and increases cell death after traumatic brain injury in mice.
abstract: Traumatic brain injury (TBI) is the leading cause of mortality and disability among male adolescents and young adults; and mild traumatic brain injury is the most common type of traumatic brain injury. The disruption of blood-brain barrier (BBB) plays an important role in brain trauma. Previously, we have found that slit2, a member of slit protein family, increases permeability of BBB. In the present study, we examined the role of slit2 in the pathogenesis of mild TBI in a mouse model of micro TBI. Rhodamine BandPI (PropidiumIodide) staining were used to detect the permeability of BBB and cell death, respectively. The leakage of Rhodamine B and cell death were significantly increased in Slit2-Tg mice than in C57 control mice after micro TBI. The present results suggest that over expression of slit2 plays a detrimental role in the pathophysiology of mild TBI.
authors: Guangdong Laboratory Animals Monitoring Institute, Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, China; Vascular Biology Research Institute, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou,China.
authors: Shuai
authors: S
authors: Li
authors: Guangdong Laboratory Animals Monitoring Institute, Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, China.
authors: Hang
authors: H
authors: Li
authors: Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors: Xiao-Fei
authors: XF
authors: He
authors: Guangdong Laboratory Animals Monitoring Institute, Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, China.
authors: Ge
authors: G
authors: Li
authors: Department of Rehabilitation Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors: Qun
authors: Q
authors: Zhang
authors: Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors: Feng-Ying
authors: FY
authors: Liang
authors: Guangdong Laboratory Animals Monitoring Institute, Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, China.
authors: Huan-Huan
authors: HH
authors: Jia
authors: Vascular Biology Research Institute, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou,China.
authors: Jiang-Chao
authors: JC
authors: Li
authors: Guangdong Laboratory Animals Monitoring Institute, Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, China.
authors: Ren
authors: R
authors: Huang
authors: Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors: Zhong
authors: Z
authors: Pei
authors: Vascular Biology Research Institute, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou,China. Electronic address: wanglijing62@163.com.
authors: Li-Jing
authors: LJ
authors: Wang
authors: Guangdong Laboratory Animals Monitoring Institute, Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, China. Electronic address: zhangyugzh@hotmail.com.
authors: Yu
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism*; D000070642:Brain Injuries, Traumatic / Q000378:metabolism*; D016923:Cell Death; D004195:Disease Models, Animal; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D000098552:Slit Homolog 2 Protein
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D000098552:Slit Homolog 2 Protein
keywords: Blood brain barrier (BBB); Cell death; Micro traumatic brain injury (TBI); Silt2; Transgenic mice