title: Target-catalyzed autonomous assembly of dendrimer-like DNA nanostructures for enzyme-free and signal amplified colorimetric nucleic acids detection.
abstract: Self-assembly of DNA nanostructures is of great importance in nanomedicine, nanotechnology and biosensing. Herein, a novel target-catalyzed autonomous assembly pathway for the formation of dendrimer-like DNA nanostructures that only employing target DNA and three hairpin DNA probes was proposed. We use the sticky-ended Y shape DNA (Y-DNA) as the assembly monomer and it was synthesized by the catalyzed hairpin assembly (CHA) instead of the DNA strand annealing method. The formed Y-DNA was equipped with three ssDNA sticky ends and two of them were predesigned to be complementary to the third one, then the dendrimer-like DNA nanostructures can be obtained via an autonomous assembly among these sticky-ended Y-DNAs. The resulting nanostructure has been successfully applied to develop an enzyme-free and signal amplified gold nanoparticle (AuNP)-based colorimetric nucleic acids assay.
authors: College of Chemistry, Sichuan University, Chengdu, 610064 China.
authors: Hongfei
authors: H
authors: He
authors: College of Chemistry, Sichuan University, Chengdu, 610064 China. Electronic address: daijy@scu.edu.cn.
authors: Jianyuan
authors: J
authors: Dai
authors: College of Chemistry, Sichuan University, Chengdu, 610064 China.
authors: Zhijuan
authors: Z
authors: Duan
authors: College of Chemical Engineering, Sichuan University, Chengdu, 610065 China.
authors: Yan
authors: Y
authors: Meng
authors: College of Chemistry, Sichuan University, Chengdu, 610064 China.
authors: Cuisong
authors: C
authors: Zhou
authors: College of Chemistry, Sichuan University, Chengdu, 610064 China.
authors: Yuyin
authors: Y
authors: Long
authors: College of Chemistry, Sichuan University, Chengdu, 610064 China.
authors: Baozhan
authors: B
authors: Zheng
authors: College of Chemistry, Sichuan University, Chengdu, 610064 China.
authors: Juan
authors: J
authors: Du
authors: College of Chemistry, Sichuan University, Chengdu, 610064 China. Electronic address: guoy@scu.edu.cn.
authors: Yong
authors: Y
authors: Guo
authors: College of Chemistry, Sichuan University, Chengdu, 610064 China; College of Chemical Engineering, Sichuan University, Chengdu, 610065 China. Electronic address: xiaodan@scu.edu.cn.
authors: Dan
authors: D
authors: Xiao
mesh_terms: D002384:Catalysis; D003124:Colorimetry / Q000295:instrumentation*; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D050091:Dendrimers / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes; D050091:Dendrimers; D009696:Nucleic Acids
keywords: Autonomous assembly; Colorimetric detection; Dendrimer-like DNA nanostructure; Enzyme-free; Nucleic acid; Signal amplification