title: A new Western blot assay for the detection of porcine cytomegalovirus (PCMV).
abstract: Porcine cytomegalovirus (PCMV) may be harmful for human recipients if xenotransplantation using pig cell, tissue or organ will be performed transmitting the virus from donor pigs to human recipients. PCMV is widespread in pigs and closely related to human pathogenic herpesviruses, however there are no data concerning infection of humans. In contrast, recently it had been shown that transplantation of organs from pigs infected with PCMV into non-human primate recipients resulted in a significant reduction of the survival time compared with the transplantation of organs from uninfected pigs. To prevent transmission of PCMV in future pig to human xenotransplantations, sensitive and specific detection methods should be used. Here a new Western blot assay using recombinant proteins corresponding to two domains of the glycoprotein gB of PCMV is described. With this assay, the presence of PCMV-specific antibodies in different pig breeds was analysed. Antibodies were detected in a high percentage of animals, in one breed up to 85%.
authors: HIV and Other Retroviruses, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany. Electronic address: PlotzkiE@rki.de.
authors: Elena
authors: E
authors: Plotzki
authors: HIV and Other Retroviruses, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany. Electronic address: KellerM@rki.de.
authors: Martina
authors: M
authors: Keller
authors: HIV and Other Retroviruses, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany. Electronic address: IvanusicD@rki.de.
authors: Daniel
authors: D
authors: Ivanusic
authors: HIV and Other Retroviruses, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany. Electronic address: DennerJ@rki.de.
authors: Joachim
authors: J
authors: Denner
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000517:prevention & control; D006801:Humans; D016377:Organ Transplantation; D011994:Recombinant Proteins / Q000235:genetics; D013552:Swine; D014183:Transplantation, Heterologous; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus
keywords: Herpesviruses; Pig; Porcine cytomegalovirus; Western blot; Xenotransplantation