title: Detection of Serum microRNAs From Department of Defense Serum Repository: Correlation With Cotinine, Cytokine, and Polycyclic Aromatic Hydrocarbon Levels.
abstract: The aim of this study was to investigate whether serum samples from the Department of Defense Serum Repository (DoDSR) are of sufficient quality to detect microRNAs (miRNAs), cytokines, immunoglobulin E (IgE), and polycyclic aromatic hydrocarbons (PAHs).
  
  
  
  MiRNAs were isolated and quantified by polymerase chain reaction (PCR) array. Cytokines and chemokines related to inflammation were measured using multiplex immunoassays. Cotinine and IgE were detected by enzyme-linked immunoassay (ELISA) and PAHs were detected by Liquid Chromatography/Mass Spectroscopy.
  
  
  
  We detected miRNAs, cytokines, IgE, and PAHs with high sensitivity. Eleven of 30 samples tested positive for cotinine suggesting tobacco exposure. Significant associations between serum cotinine, cytokine, IgE, PAHs, and miRNA were discovered.
  
  
  
  We successfully quantified over 200 potential biomarkers of occupational exposure from DoDSR samples. The stored serum samples were not affected by hemolysis and represent a powerful tool for biomarker discovery and analysis in retrospective studies.
authors: Department of Environmental Medicine (Dr Woeller, Mr Pollock, Ms Croasdell, Ms Kim, Dr Utell, Dr Phipps), Department of Medicine (Drs Thatcher, Utell, and Phipps), Microbiology and Immunology, University of Rochester Medical Center (Mr Van Twisk, Drs Thakar and Phipps); Center for Air Resources Engineering and Science, Clarkson University, Potsdam, New York (Drs Hopke and Xia); and Professor, Department of Preventive Medicine and Biostatistics, Uniformed Services University, Bethesda, MD 20814 (Dr Mallon).
authors: Collynn F
authors: CF
authors: Woeller
authors: Thomas H
authors: TH
authors: Thatcher
authors: Daniel
authors: D
authors: Van Twisk
authors: Stephen J
authors: SJ
authors: Pollock
authors: Amanda
authors: A
authors: Croasdell
authors: Nina
authors: N
authors: Kim
authors: Philip K
authors: PK
authors: Hopke
authors: Xiaoyan
authors: X
authors: Xia
authors: Juilee
authors: J
authors: Thakar
authors: Col Timothy M
authors: CT
authors: Mallon
authors: Mark J
authors: MJ
authors: Utell
authors: Richard P
authors: RP
authors: Phipps
mesh_terms: D015415:Biomarkers / Q000097:blood; D001771:Blood Banks; D003367:Cotinine / Q000097:blood*; D016207:Cytokines / Q000097:blood*; D004781:Environmental Exposure / Q000032:analysis; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D035683:MicroRNAs / Q000097:blood*; D008889:Military Personnel; D016273:Occupational Exposure / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000097:blood*; D012189:Retrospective Studies
chemical_list: D015415:Biomarkers; D016207:Cytokines; D035683:MicroRNAs; D011084:Polycyclic Aromatic Hydrocarbons; D007073:Immunoglobulin E; D003367:Cotinine