title: 3D structure of eukaryotic flagella/cilia by cryo-electron tomography.
abstract: Flagella/cilia are motile organelles with more than 400 proteins. To understand the mechanism of such complex systems, we need methods to describe molecular arrange-ments and conformations three-dimensionally in vivo. Cryo-electron tomography enabled us such a 3D structural analysis. Our group has been working on 3D structure of flagella/cilia using this method and revealed highly ordered and beautifully organized molecular arrangement. 3D structure gave us insights into the mechanism to gener-ate bending motion with well defined waveforms. In this review, I summarize our recent structural studies on fla-gella/cilia by cryo-electron tomography, mainly focusing on dynein microtubule-based ATPase motor proteins and the radial spoke, a regulatory protein complex.
authors: Laboratory of Biomolecular Research, Paul Scherrer Institute, Villigen PSI, CH5232, Switzerland.
authors: Takashi
authors: T
authors: Ishikawa
keywords: axoneme; cryo-EM; dynein; microtubule; motor protein