title: Novel super-resolution capable mitochondrial probe, MitoRed AIE, enables assessment of real-time molecular mitochondrial dynamics.
abstract: Mitochondria and mitochondrial dynamics play vital roles in health and disease. With the intricate nanometer-scale structure and rapid dynamics of mitochondria, super-resolution microscopy techniques possess great un-tapped potential to significantly contribute to understanding mitochondrial biology and kinetics. Here we present a novel mitochondrial probe (MitoRed AIE) suitable for live mitochondrial dynamics imaging and single particle tracking (SPT), together with a multi-dimensional data analysis approach to assess local mitochondrial (membrane) fluidity. The MitoRed AIE probe localizes primarily to mitochondrial membranes, with 95 ms fluorophore on-time delivering 106 photons/ms, characteristics which we exploit to demonstrate live cell 100 fps 3D time-lapse tracking of mitochondria. Combining our experimental and analytical approaches, we uncover mitochondrial dynamics at unprecedented time scales. This approach opens up a new regime into high spatio-temporal resolution dynamics in many areas of mitochondrial biology.
authors: Monash Micro Imaging, Monash University, Melbourne, Australia.|Hudson Institute of Medical Research, Clayton, Victoria, Australia.
authors: Camden Yeung-Wah
authors: CY
authors: Lo
authors: School of Chemistry, University of Melbourne, Melbourne, Australia.|Division of Biomedical Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
authors: Sijie
authors: S
authors: Chen
authors: Monash Micro Imaging, Monash University, Melbourne, Australia.|Hudson Institute of Medical Research, Clayton, Victoria, Australia.
authors: Sarah Jayne
authors: SJ
authors: Creed
authors: Division of Biomedical Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
authors: Miaomiao
authors: M
authors: Kang
authors: School of Chemistry &Chemical Engineering, Shaanxi Normal University, P.R. China.
authors: Na
authors: N
authors: Zhao
authors: Division of Biomedical Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
authors: Ben Zhong
authors: BZ
authors: Tang
authors: Monash Micro Imaging, Monash University, Melbourne, Australia.|Hudson Institute of Medical Research, Clayton, Victoria, Australia.
authors: Kirstin Diana
authors: KD
authors: Elgass
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D008560:Membrane Fluidity / Q000502:physiology; D008856:Microscopy, Fluorescence / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism*; D063154:Mitochondrial Dynamics / Q000502:physiology*; D051336:Mitochondrial Membranes / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes