title: Tissue-engineered human psoriatic skin supplemented with cytokines as an in vitro model to study plaque psoriasis.
abstract: Psoriasis is a chronic inflammatory skin disease. To study its complex etiology, a psoriatic skin substitute model supplemented with a cytokine cocktail has been used.
  
  
  
  Reconstructed psoriatic skin substitutes were supplemented with a cocktail of four cytokines: TNF-α, IL-1α, IL-6 and IL-17A, to monitor their impact on gene expression by DNA microarray.
  
  
  
  Gene profiling analyses identified several deregulated genes reported as being also deregulated in psoriasis skin in vivo (S100A12, IL-8, DEFB4A and KYNU). The expression of those genes was dramatically increased compared with basal levels of controls (p < 0.005 to < 0.05).
  
  
  
  Psoriatic substitutes supplemented with a cocktail of TNF-α, IL-1α, IL-6 and IL-17A showed similar transcriptome alterations to those found in psoriasis.
authors: Centre LOEX de l'Université Laval, Génie Tissulaire et Régénération, Centre de Recherche FRQS du CHU de Québec, Axe Médecine Régénératrice, Québec, QC, Canada.|Faculté de Pharmacie, Université Laval, Québec, QC, Canada.
authors: Claudia
authors: C
authors: Pouliot-Bérubé
authors: Centre Universitaire d'Ophtalmologie-Recherche, Centre de Recherche FRQS du CHU de Québec, Axe Médecine Régénératrice, Québec, QC, Canada.
authors: Karine
authors: K
authors: Zaniolo
authors: Centre Universitaire d'Ophtalmologie-Recherche, Centre de Recherche FRQS du CHU de Québec, Axe Médecine Régénératrice, Québec, QC, Canada.|Département d'Ophtalmologie, Université Laval, Québec, QC, Canada.
authors: Sylvain L
authors: SL
authors: Guérin
authors: Centre LOEX de l'Université Laval, Génie Tissulaire et Régénération, Centre de Recherche FRQS du CHU de Québec, Axe Médecine Régénératrice, Québec, QC, Canada.|Faculté de Pharmacie, Université Laval, Québec, QC, Canada.
authors: Roxane
authors: R
authors: Pouliot
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D012867:Skin / Q000166:cytology* / Q000187:drug effects / Q000294:innervation; D016038:Skin Transplantation; D017768:Skin, Artificial / Q000706:statistics & numerical data*; D023822:Tissue Engineering
chemical_list: D015415:Biomarkers; D016207:Cytokines
keywords: cytokines gene ontology analysis; immunology biotechnology; psoriasis transcriptome; skin gene expression profiling; tissue engineering immune response