title: Variation in ruminal in situ degradation of crude protein and starch from maize grains compared to in vitro gas production kinetics and physical and chemical characteristics.
abstract: The objectives of this study were (1) to evaluate in situ ruminal dry matter (DM), crude protein (CP) and starch degradation characteristics and in vitro gas production (GP) kinetics using a set of 20 different maize grain genotypes and (2) to predict the effective degradation (ED) of CP and starch from chemical and physical characteristics alone or in combination with in vitro GP measurements. Maize grains were characterised by different chemical and physical characteristics. Ruminal in situ degradation was measured in three lactating Jersey cows. Ground grains (sieve size: 2 mm) were incubated in bags for 1, 2, 4, 8, 16, 24, 48 and 72 h. Bag residues were analysed for CP and starch content. Degradation kinetics was determined and the ED of DM, CP and starch calculated using a ruminal passage rate of 5%/h and 8%/h. The GP of the grains (sieve size: 1 mm) was recorded after 2, 4, 6, 8, 12, 24, 48 and 72 h incubation in buffered rumen fluid and fitted to an exponential equation to determine GP kinetics. Correlations and stepwise multiple linear regressions were evaluated for the prediction of ED calculated for a passage rate of 5%/h (ED5) for CP (EDCP5) and starch (EDST5). The in situ parameters and ED5 varied widely between genotypes with average values (±SD) of 64% ± 4.2, 62% ± 4.1 and 65% ± 5.2 for ED5 of DM, EDCP5 and EDST5 and were on average 10 percentage points lower for a passage rate of 8%/h. Degradation rates varied between 4.8%/h and 7.4%/h, 4.1%/h and 6.5%/h and 5.3%/h and 8.9%/h for DM, CP and starch, respectively. These rates were in the same range as GP rates (6.0-8.3%/h). The EDCP5 and EDST5 were related to CP concentration and could be evaluated in detail using CP fractions and specific amino acids. In vitro GP measurements and GP rates correlated well with EDCP5 and EDST5 and predicted EDCP5 and EDST5 in combination with the chemical characteristics of the samples. Equations can be used to obtain quick and cost effective information on ruminal degradation of CP and starch from maize grains.
authors: a Institut für Nutztierwissenschaften , Universität Hohenheim , Stuttgart , Germany.
authors: Natascha
authors: N
authors: Seifried
authors: a Institut für Nutztierwissenschaften , Universität Hohenheim , Stuttgart , Germany.
authors: Herbert
authors: H
authors: Steingaß
authors: b Institut für Pflanzenzüchtung, Saatgutforschung und Populationsgenetik , Universität Hohenheim.
authors: Wolfgang
authors: W
authors: Schipprack
authors: a Institut für Nutztierwissenschaften , Universität Hohenheim , Stuttgart , Germany.
authors: Markus
authors: M
authors: Rodehutscord
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004032:Diet / Q000662:veterinary; D004040:Dietary Carbohydrates / Q000378:metabolism*; D004044:Dietary Proteins / Q000378:metabolism*; D002523:Edible Grain / Q000737:chemistry; D005740:Gases / Q000378:metabolism*; D005766:Gastrointestinal Contents / Q000737:chemistry*; D012417:Rumen / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry* / Q000235:genetics
chemical_list: D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D005740:Gases
keywords: Gas production; genotypic variation; in vitro production; maize; prediction; rumen fermentation