title: Studies on organic and in-organic biostimulants in bioremediation of diesel-contaminated arable soil.
abstract: In this study, use of inorganic fertilizer (N.P.K) was compared with organic manure (compost) in the bioremediation of diesel-polluted agricultural soil over a two-month period. Renewal by enhanced natural attenuation was used as control. The results revealed that total petroleum hydrocarbon removal from polluted soil was 71.40 ± 5.60% and 93.31 ± 3.60% for N.P.K and compost amended options, respectively. The control (natural attenuation) had 57.90 ± 3.98% of total petroleum hydrocarbon removed. Experimental data fitted second order kinetic model adequately for compost amended option. The fertilizer amended option was found to be 1.04 times slower (k2 = 4.00 ± 1.40 × 10(-7)gmg(-1)d(-1), half-life = 28.15 d) than compost amended option (k2 = 1.39 ± 0.54 × 10(-5) gmg(-1)d(-1), half-life = 8.10 d) but 1.21 times (20.6%) faster than the control (k2 = 2.57 ± 0.16 × 10(-7) gmg(-1)d(-1), half-life = 43.81 d). The hydrocarbon utilizers isolated from the diesel contaminated soil were: Bacillus nealsoni, Micrococcus luteus, Aspergillus awamori, and Fusarium proliferatum. The phytotoxicity test showed that germination indices for natural attenuation (control), fertilizer (NPK) and compost amended options were 34%, 56%, and 89%, respectively.
authors: Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, PMB 5025, Awka, Anambra State, Nigeria. Electronic address: smetchie@yahoo.com.
authors: Amechi S
authors: AS
authors: Nwankwegu
authors: Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, PMB 5025, Awka, Anambra State, Nigeria.
authors: Michael U
authors: MU
authors: Orji
authors: Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
authors: Chukwudi O
authors: CO
authors: Onwosi
mesh_terms: D000383:Agriculture; D001673:Biodegradation, Environmental; D054750:Ecotoxicology; D005308:Fertilizers / Q000382:microbiology* / Q000633:toxicity; D018525:Germination / Q000187:drug effects; D006207:Half-Life; D007700:Kinetics; D008372:Manure / Q000382:microbiology*; D009549:Nigeria; D010578:Petroleum / Q000032:analysis*; D012987:Soil / Q000737:chemistry / Q000592:standards; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*; D003313:Zea mays / Q000187:drug effects / Q000254:growth & development
chemical_list: D005308:Fertilizers; D008372:Manure; D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants
keywords: Compost; Ecotoxicity; Fertilizer; Natural attenuation; Total petroleum hydrocarbon (TPH)