title: Decreased fucosylated PSA as a urinary marker for high Gleason score prostate cancer.
abstract: Fucosylation is an important oligosaccharide modification associated with cancer and inflammation. We investigated whether urinary fucosylated PSA (Fuc-PSA) levels could be used for the detection of high Gleason score prostate cancer. Urine samples were collected from men with abnormal digital rectal examination findings or elevated serum PSA levels, before prostate biopsy. Lectin-antibody ELISA was used to quantify the Lewis-type or core-type fucosylated PSA (PSA-AAL) and core-type fucosylated PSA (PSA-PhoSL) in the urine samples. Both types of urinary Fuc-PSA were significantly decreased in the men with prostate cancer compared with the men whose biopsies were negative for cancer (P = 0.026 and P < 0.001, respectively). Both were also significantly associated with the Gleason scores of the biopsy specimens (P = 0.001 and P < 0.001, respectively). Multivariate analysis showed that PSA density, urinary PSA-AAL, and urinary PSA-PhoSL were independent predictors of high Gleason score prostate cancer. The area under the receiver-operator characteristic curve (AUC) value for the prediction of cancers of Gleason score ≥ 7 was 0.69 for urinary PSA-AAL and 0.72 for urinary PSA-PhoSL. In contrast, the AUC value was 0.59 for serum PSA, 0.63 for PSA density, and 0.58 for urinary PSA. In conclusion, a decreased urinary Fuc-PSA level is a potential marker for the detection of high Gleason score prostate cancer.
authors: Department of Urology, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Kazutoshi
authors: K
authors: Fujita
authors: Department of Urology, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Takuji
authors: T
authors: Hayashi
authors: Department of Urology, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Kyosuke
authors: K
authors: Matsuzaki
authors: Department of Urology, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Wataru
authors: W
authors: Nakata
authors: Department of Molecular Biochemistry and Clinical Investigation, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Mika
authors: M
authors: Masuda
authors: Department of Urology, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Atsunari
authors: A
authors: Kawashima
authors: Department of Urology, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Takeshi
authors: T
authors: Ujike
authors: Department of Urology, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Akira
authors: A
authors: Nagahara
authors: Department of Urology, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Mutsumi
authors: M
authors: Tsuchiya
authors: J-Oil Mills, Inc., Yokohama, Japan.
authors: Yuka
authors: Y
authors: Kobayashi
authors: Department of Pathology, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Satoshi
authors: S
authors: Nojima
authors: Department of Urology, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Motohide
authors: M
authors: Uemura
authors: Department of Pathology, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Eiichi
authors: E
authors: Morii
authors: Department of Molecular Biochemistry and Clinical Investigation, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Eiji
authors: E
authors: Miyoshi
authors: Department of Urology, Osaka University Graduate School of Medicine, Suita, Japan.
authors: Norio
authors: N
authors: Nonomura
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000652:urine*; D001706:Biopsy; D004797:Enzyme-Linked Immunosorbent Assay; D005643:Fucose / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000737:chemistry; D007983:Lewis Blood Group Antigens; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D017430:Prostate-Specific Antigen / Q000737:chemistry / Q000652:urine*; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000652:urine*; D012372:ROC Curve; D012044:Regression Analysis
chemical_list: D014408:Biomarkers, Tumor; D037102:Lectins; D007983:Lewis Blood Group Antigens; D005643:Fucose; D017430:Prostate-Specific Antigen
keywords: Gleason score; PSA; fucosylation; prostate cancer; urine