title: miR-346 promotes migration and invasion of nasopharyngeal carcinoma cells via targeting BRMS1.
abstract: The aim of this study is to determine the expression and roles of miR-346 in nasopharyngeal carcinoma (NPC). We showed that miR-346 was upregulated in NPC tissues compared with adjacent non-tumorous nasopharyngeal tissues. Inhibition of miR-346 significantly attenuated the migration and invasion of NPC cells. Luciferase reporter assay showed that miR-346 targeted the 3'-untranslated region (3'-UTR) of breast cancer metastasis suppressor 1 (BRMS1). Overexpression of miR-346 suppressed the endogenous expression of BRMS1 in NPC cells. There was a significant negative correlation between miR-346 and BRMS1 protein expression in NPC tissues (r = -0.372, P = 0.008). Rescue experiments demonstrated that overexpression of BRMS1 lacking the 3'-UTR impaired the invasiveness of NPC cells transfected with miR-346 mimic. Taken together, miR-346 shows the ability to promote the migration and invasion of nasopharyngeal cancer cells via targeting BRMS1 and represents a potential therapeutic target for NPC.
authors: Department of Otorhinolaryngology, Tangshan Maternity and Child Health Care Hospital, Tangshan, People's Republic of China.
authors: Hong-Ling
authors: HL
authors: Yan
authors: Department of Radiotherapy, Shanghai Armed Police Hospital, Shanghai, People's Republic of China.
authors: Li
authors: L
authors: Li
authors: Department of Otorhinolaryngology, Tangshan Maternity and Child Health Care Hospital, Tangshan, People's Republic of China.
authors: Shu-Juan
authors: SJ
authors: Li
authors: Department of Otorhinolaryngology, Tangshan Maternity and Child Health Care Hospital, Tangshan, People's Republic of China.
authors: Hai-Sheng
authors: HS
authors: Zhang
authors: Department of Neurosurgery, Huashan Hospital, Fudan University of Medicine, Shanghai, People's Republic of China.
authors: Wei
authors: W
authors: Xu
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D001665:Binding Sites; D002277:Carcinoma; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009361:Neoplasm Invasiveness; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C410024:BRMS1 protein, human; C560923:MIRN346 microRNA, human; D035683:MicroRNAs; D009841:Oligonucleotides; D012097:Repressor Proteins; D008156:Luciferases
keywords: invasion; microRNA; migration; nasopharyngeal carcinoma; target gene