title: Lactobacillus plantarum BL011 cultivation in industrial isolated soybean protein acid residue.
abstract: In this study, physiological aspects of Lactobacillus plantarum BL011 growing in a new, all-animal free medium in bioreactors were evaluated aiming at the production of this important lactic acid bacterium. Cultivations were performed in submerged batch bioreactors using the Plackett-Burman methodology to evaluate the influence of temperature, aeration rate and stirring speed as well as the concentrations of liquid acid protein residue of soybean, soy peptone, corn steep liquor, and raw yeast extract. The results showed that all variables, except for corn steep liquor, significantly influenced biomass production. The best condition was applied to bioreactor cultures, which produced a maximal biomass of 17.87gL-1, whereas lactic acid, the most important lactic acid bacteria metabolite, peaked at 37.59gL-1, corresponding to a productivity of 1.46gL-1h-1. This is the first report on the use of liquid acid protein residue of soybean medium for L. plantarum growth. These results support the industrial use of this system as an alternative to produce probiotics without animal-derived ingredients to obtain high biomass concentrations in batch bioreactors.
authors: Federal University of Rio Grande do Sul, Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Porto Alegre, RS, Brazil.
authors: Chaline Caren
authors: CC
authors: Coghetto
authors: Federal University of Rio Grande do Sul, Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Porto Alegre, RS, Brazil.
authors: Carolina Bettker
authors: CB
authors: Vasconcelos
authors: Federal University of Health Sciences of Porto Alegre, Nutrition Department, Porto Alegre, RS, Brazil.
authors: Graziela Brusch
authors: GB
authors: Brinques
authors: Federal University of Rio Grande do Sul, Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Porto Alegre, RS, Brazil. Electronic address: mazayub@ufrgs.br.
authors: Marco Antônio Záchia
authors: MA
authors: Ayub
mesh_terms: D018533:Biomass; D019149:Bioreactors; D050260:Carbohydrate Metabolism; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006868:Hydrolysis; D019344:Lactic Acid / Q000096:biosynthesis; D048191:Lactiplantibacillus plantarum / Q000254:growth & development* / Q000378:metabolism*; D030262:Soybean Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D003470:Culture Media; D030262:Soybean Proteins; D019344:Lactic Acid
keywords: Industrial production of biomass; Lactic acid; Lactobacillus plantarum BL011; Liquid acid protein residue of soybean; Plackett–Burman design