title: Plasma miR-324-3p and miR-1285 as diagnostic and prognostic biomarkers for early stage lung squamous cell carcinoma.
abstract: BACKGROUND
  Specific biomarkers for early detection and outcome prediction of lung squamous cell carcinoma (LSCC) are still lacking. This study assessed the differentially expressed miRNAs as potential biomarkers for early stage LSCC.
  
  
  RESULTS
  Base on the results of multi-phase study, we found that miR-324-3p was significantly up-regulated, whereas mir-1285 was significantly down-regulated in plasma of stage I LSCC patients compared to healthy controls. ROC analysis showed that AUC of miR-324-3p and miR-1285 were 0.79 and 0.85, respectively. The combination of these two miRNAs could further improve the diagnostic accuracy (AUC = 0.89). The multivariate analysis revealed that plasma miR-324-3p level was an independent prognostic predictor for early stage LSCC.
  
  
  METHODS
  395 patients and 195 healthy controls were enrolled in this study. We screened the differentially expressed plasma miRNAs using TaqMan Low Density Arrays (TLDA) followed by three-phase qRT-PCR validation. We also evaluated the association of candidate miRNAs with overall survival of early stage LSCC patients. Finally, the target genes of the candidate miRNAs were analyzed using public available databases and bioinformatics methods.
  
  
  CONCLUSIONS
  The current study suggests that plasma miR-324-3p and miR-1285 levels could serve as LSCC early detection markers while miR-324-3p may serve as a prognostic marker for LSCC patients.
authors: Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.|National Clinical Research Center of Cancer, Tianjin, China.|Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, China.
authors: Xujie
authors: X
authors: Gao
authors: Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.|National Clinical Research Center of Cancer, Tianjin, China.|Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, China.
authors: Yang
authors: Y
authors: Wang
authors: Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.|National Clinical Research Center of Cancer, Tianjin, China.|Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, China.
authors: Hua
authors: H
authors: Zhao
authors: Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.|National Clinical Research Center of Cancer, Tianjin, China.|Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, China.
authors: Feng
authors: F
authors: Wei
authors: Department of Biotherapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.|National Clinical Research Center of Cancer, Tianjin, China.|Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, China.
authors: Xinwei
authors: X
authors: Zhang
authors: National Clinical Research Center of Cancer, Tianjin, China.|Department of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
authors: Yanjun
authors: Y
authors: Su
authors: National Clinical Research Center of Cancer, Tianjin, China.|Department of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
authors: Changli
authors: C
authors: Wang
authors: Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.|National Clinical Research Center of Cancer, Tianjin, China.|Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, China.
authors: Hui
authors: H
authors: Li
authors: Department of Biotherapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.|National Clinical Research Center of Cancer, Tianjin, China.|Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, China.
authors: Xiubao
authors: X
authors: Ren
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics*; D042241:Early Diagnosis; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D012372:ROC Curve
chemical_list: D014408:Biomarkers, Tumor; C572554:MIRN1285 microRNA, human; C000599310:MIRN324 microRNA, human; D035683:MicroRNAs
keywords: diagnostic marker; lung squamous cell carcinoma; miRNA; plasma; prognostic marker